Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers.
Burkholderia is an incredibly diverse and versatile Gram-negative genus, within which over 80 species have been formally named and multiple other genotypic groups likely represent new species. Phylogenetic analysis based on the 16S rRNA gene sequence and core genome ribosomal multilocus sequence typing analysis indicates the presence of at least three major clades within the genus. Biotechnologically, Burkholderia are well-known for their bioremediation and biopesticidal properties. Within this review, we explore the ability of Burkholderia to synthesise a wide range of antimicrobial compounds ranging from historically characterised antifungals to recently described antibacterial antibiotics with activity against multiresistant clinical pathogens. The production of multiple Burkholderia antibiotics is controlled by quorum sensing and examples of quorum sensing pathways found across the genus are discussed. The capacity for antibiotic biosynthesis and secondary metabolism encoded within Burkholderia genomes is also evaluated. Overall, Burkholderia demonstrate significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites.
Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000, Ghent, Belgium.|Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium.
Eliza
E
Depoorter
Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Main Building, Museum Avenue, Cardiff, UK.
Matt J
MJ
Bull
Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000, Ghent, Belgium.
Charlotte
C
Peeters
Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, B-9000, Ghent, Belgium.
Tom
T
Coenye
Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000, Ghent, Belgium. Peter.Vandamme@UGent.be.
Peter
P
Vandamme
Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Main Building, Museum Avenue, Cardiff, UK. MahenthiralingamE@cardiff.ac.uk.
Eshwar
E
Mahenthiralingam
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000935:Antifungal Agents / Q000378:metabolism*; D015373:Bacterial Typing Techniques / Q000379:methods; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D020459:Genes, rRNA; D005838:Genotype; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D053038:Quorum Sensing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D064210:Secondary Metabolism; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
Antibiotic biosynthesis; Burkholderia; Genomics; Phylogeny; Secondary metabolism; Taxonomy