Rapid detection of measles virus using reverse transcription loop-mediated isothermal amplification coupled with a disposable lateral flow device.
The measles virus (MeV) causes a highly contagious disease and efforts to reduce its spread are critical. A reverse transcription loop-mediated isothermal amplification assay coupled with a disposable lateral flow device (RT-LAMP-LFD) was developed for the rapid detection of MeV. The assay was performed in 40 min at an optimal temperature of 58 °C, with endpoint results visualized directly. A probe that was complementary to the RT-LAMP amplicon was designed to enhance assay specificity. Detection limit of the assay was 8.8 copies/μL synthetic RNA, which equals the sensitivity of real-time RT-PCR. Clinical specimens were used to validate the RT-LAMP-LFD in provincial Center for Disease Control and Prevention (CDC) (n = 245) and six municipal CDCs (n = 249). The results obtained using RT-LAMP-LFD and real-time RT-PCR were highly concordant. The RT-LAMP-LFD is rapid, stable, and does not require expensive equipment, which can be used for routine MeV monitoring in CDC laboratories.
Key Lab. of emergency detection for Public Health of Zhejiang province, Zhejiang provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, People's Republic of China.
Changping
C
Xu
Key Lab. of emergency detection for Public Health of Zhejiang province, Zhejiang provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, People's Republic of China.
Yan
Y
Feng
Key Lab. of emergency detection for Public Health of Zhejiang province, Zhejiang provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, People's Republic of China.
Yin
Y
Chen
Key Lab. of emergency detection for Public Health of Zhejiang province, Zhejiang provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, People's Republic of China.
Jian
J
Gao
Key Lab. of emergency detection for Public Health of Zhejiang province, Zhejiang provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, People's Republic of China. Electronic address: luyiyuzjh@163.com.
Yiyu
Y
Lu
D006801:Humans; D008457:Measles / Q000175:diagnosis*; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation* / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
D015345:Oligonucleotide Probes
Lateral flow device; Measles virus; Rapid detection; Reverse transcription loop-mediated isothermal amplification