CFD investigation of Schizochytrium sp. impeller configurations on cell growth and docosahexaenoic acid synthesis.
Effects of impeller configurations on docosahexaenoic acid production and flow characteristics were investigated by Schizochytrium sp. in a 15 L bioreactor. 6-straight blade disc turbine (6-SBDT), 6-arrowy-blade disc turbine (6-ABDT) and down-pumping propeller (DPP) were combined to form different impeller configurations. Simulated results showed that configuration SSA consisting of upper two 6-SBDT and one bottom 6-ABDT possessed the worst oxygen supply capacity. But it obtained the highest DHA percentage of 48.17 % and DHA yield of 21.42 g/L, indicating that it was beneficial for DHA synthesis and converting glucose to biomass and lipids. Configuration SAS consisting of one middle 6-ABDT and two 6-SBDT provided better mixing capacity, which resulted in the maximum glucose consumption rate of 2.86 g/L h and the highest biomass of 108.09 g/L. This study would improve insight into understanding the relationship between flow field and the physiology of Schizochytrium sp. for the scale-up of industrial DHA production.
Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
Xiaoyan
X
Zhao
Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China. renlujing@njtech.edu.cn.
Lujing
L
Ren
Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
Dongsheng
D
Guo
Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
Wenjia
W
Wu
Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
Xiaojun
X
Ji
Jiangsu National Synergetic Innovation Center for Advanced Materials, College of Biotechnology and Pharmaceutical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China. biotech@njtech.edu.cn.
He
H
Huang
D019149:Bioreactors; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005285:Fermentation; D058009:Stramenopiles / Q000254:growth & development* / Q000378:metabolism
D004281:Docosahexaenoic Acids
CFD simulation; Docosahexaenoic acid; Fluid mechanics; Impeller; Schizochytrium sp.