Expression of Genes with Copy Number Alterations and Survival of Patients with Pancreatic Adenocarcinoma.
OBJECTIVE
Individual molecular information might improve management of pancreatic adenocarcinoma. To identify actionable genes, at the transcriptional level, we investigated candidate genes that we had previously identified using array-comparative genomic hybridization (aCGH).


METHODS
We collected 10 public gene-expression datasets, gathering a total of 524 pancreatic samples (105 normal and 419 malignant tissues). Based on our previous aCGH analysis, we searched for genes differentially expressed between normal and malignant samples and genes associated with survival.


RESULTS
Among genes amplified/gained by aCGH, 48% were overexpressed in malignant tissues. The majority of these genes were related to apoptosis, cell-cycle regulation and differentiation. Among genes located in areas of loss, 41% were underexpressed in malignant tissues; most of them were involved in ion transport, homeostasis maintenance and fatty acid metabolism. Survival analysis identified genes significantly related to shorter (n=17) or longer (n=29) survival.


CONCLUSIONS
Some of these genes can be further investigated as potential prognostic markers.
Department of Molecular Oncology UMR1068, Paoli-Calmettes Instituet, Marseille, France Department of Digestive Surgery, North Hospital, Marseille, France Aix-Marseille University, Marseille, France david.birnbaum10@gmail.com.
David J
DJ
Birnbaum
Department of Molecular Oncology UMR1068, Paoli-Calmettes Instituet, Marseille, France Aix-Marseille University, Marseille, France Department of Medical Oncology, Paoli-Calmettes Instituet, Marseille, France.
François
F
Bertucci
Department of Molecular Oncology UMR1068, Paoli-Calmettes Instituet, Marseille, France.
Pascal
P
Finetti
Department of Molecular Oncology UMR1068, Paoli-Calmettes Instituet, Marseille, France.
José
J
Adélaïde
Department of Gastroenterology, Paoli-Calmettes Instituet, Marseille, France.
Marc
M
Giovannini
Aix-Marseille University, Marseille, France Department of Digestive and Oncologic Surgery, Marseille Research Center of Cancerology UMR1068, Paoli-Calmettes Instituet, Marseille, France.
Olivier
O
Turrini
Aix-Marseille University, Marseille, France Department of Digestive and Oncologic Surgery, Marseille Research Center of Cancerology UMR1068, Paoli-Calmettes Instituet, Marseille, France.
Jean Robert
JR
Delpero
Aix-Marseille University, Marseille, France Department of Medical Oncology, Paoli-Calmettes Instituet, Marseille, France.
Jean Luc
JL
Raoul
Department of Molecular Oncology UMR1068, Paoli-Calmettes Instituet, Marseille, France.
Max
M
Chaffanet
Department of Digestive Surgery, North Hospital, Marseille, France Aix-Marseille University, Marseille, France.
Vincent
V
Moutardier
Department of Molecular Oncology UMR1068, Paoli-Calmettes Instituet, Marseille, France.
Daniel
D
Birnbaum
Department of Molecular Oncology UMR1068, Paoli-Calmettes Instituet, Marseille, France Aix-Marseille University, Marseille, France.
Emilie
E
Mamessier
D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality*; D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D055028:Comparative Genomic Hybridization; D019295:Computational Biology / Q000379:methods; D056915:DNA Copy Number Variations; D030541:Databases, Genetic; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D011379:Prognosis; D055815:Young Adult
aCGH; apoptosis; cell-cycle regulation; copy number alterations; pancreatic adenocarcinoma