Simple detection of residual enrofloxacin in meat products using microparticles and biochips.
A simple and sensitive method for detecting enrofloxacin, a major veterinary fluoroquinolone, was developed. Monoclonal antibody specific for enrofloxacin was immobilised on a chip and fluorescent dye-labelled microparticles were covalently bound to the enrofloxacin molecules. Enrofloxacin in solution competes with the microparticle-immobilised enrofloxacin (enroMPs) to bind to the antibody on the chip. The presence of enrofloxacin was verified by detecting the fluorescence of enrofloxacin-bound microparticles. Under optimum conditions, a high dynamic range was achieved at enrofloxacin concentrations ranging from 1 to 1000 μg kg(-1). The limits of detection and quantification for standard solutions were 5 and 20 μg kg(-1) respectively, which are markedly lower than the maximum residue limit. Using simple extraction methods, recoveries from fortified beef, pork and chicken samples were 43.4-62.3%. This novel method also enabled approximate quantification of enrofloxacin concentration: the enroMP signal intensity decreased with increasing enrofloxacin concentration. Because of its sensitivity, specificity, simplicity and rapidity, the method described herein will facilitate the detection and approximate quantification of enrofloxacin residues in foods in a high-throughput manner.
a Department of Bioscience and Biotechnology , Konkuk University , Seoul , Korea.
Mi-Sun
MS
Ha
b School of Food Science & Technology , Chung-Ang University , Anseong , Korea.
Myung-Sub
MS
Chung
a Department of Bioscience and Biotechnology , Konkuk University , Seoul , Korea.
Dong-Ho
DH
Bae
D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000970:Antineoplastic Agents / Q000032:analysis*; D001667:Binding, Competitive; D002417:Cattle; D002645:Chickens; D004350:Drug Residues / Q000032:analysis*; D000077422:Enrofloxacin; D005456:Fluorescent Dyes / Q000737:chemistry; D024841:Fluoroquinolones / Q000032:analysis*; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D057166:High-Throughput Screening Assays; D057230:Limit of Detection; D008460:Meat / Q000032:analysis* / Q000191:economics; D046208:Microchip Analytical Procedures; D008863:Microspheres; D015203:Reproducibility of Results; D056910:Republic of Korea; D034421:Sus scrofa
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D024841:Fluoroquinolones; D000077422:Enrofloxacin
Enrofloxacin; antibody; biochips; microparticles