Microbiota-based Signature of Gingivitis Treatments: A Randomized Study.
Plaque-induced gingivitis can be alleviated by various treatment regimens. To probe the impacts of various anti-gingivitis treatments on plaque microflora, here a double blinded, randomized controlled trial of 91 adults with moderate gingivitis was designed with two anti-gingivitis regimens: the brush-alone treatment and the brush-plus-rinse treatment. In the later group, more reduction in both Plaque Index (TMQHI) and Gingival Index (mean MGI) at Day 3, Day 11 and Day 27 was evident, and more dramatic changes were found between baseline and other time points for both supragingival plaque microbiota structure and salivary metabonomic profiles. A comparison of plaque microbiota changes was also performed between these two treatments and a third dataset where 50 subjects received regimen of dental scaling. Only Actinobaculum, TM7 and Leptotrichia were consistently reduced by all the three treatments, whereas the different microbial signatures of the three treatments during gingivitis relieve indicate distinct mechanisms of action. Our study suggests that microbiota based signatures can serve as a valuable approach for understanding and potentially comparing the modes of action for clinical treatments and oral-care products in the future.
Single-Cell Center and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.|Sino-Danish Center for Education and Research, Beijing, 100190, China.|University of Chinese Academy of Sciences, Beijing, 100190, China.
Shi
S
Huang
Department of Stomatology, Peking Union Medical College Hospital, Beijing, 100730, China.
Zhen
Z
Li
Procter &Gamble Mason Business Center, Cincinnati, OH 45040, USA.
Tao
T
He
Single-Cell Center and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
Cunpei
C
Bo
Procter &Gamble Innovation Center, Beijing 101312, China.
Jinlan
J
Chang
Procter &Gamble Innovation Center, Beijing 101312, China.
Lin
L
Li
Procter &Gamble Innovation Center, Beijing 101312, China.
Yanyan
Y
He
Procter &Gamble International Operations SA SG Branch, 138547, Singapore.
Jiquan
J
Liu
Procter &Gamble Mason Business Center, Cincinnati, OH 45040, USA.
Duane
D
Charbonneau
Procter &Gamble International Operations SA SG Branch, 138547, Singapore.
Rui
R
Li
Single-Cell Center and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
Jian
J
Xu
D000293:Adolescent; D000328:Adult; D044822:Biodiversity; D016000:Cluster Analysis; D003773:Dental Plaque / Q000382:microbiology; D003774:Dental Plaque Index; D005260:Female; D005891:Gingivitis / Q000175:diagnosis / Q000382:microbiology* / Q000628:therapy*; D006801:Humans; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D064307:Microbiota / Q000187:drug effects; D008875:Middle Aged; D009067:Mouthwashes; D012463:Saliva / Q000378:metabolism; D014099:Toothbrushing; D055815:Young Adult
D009067:Mouthwashes