Induction of antroquinonol production by addition of hydrogen peroxide in the fermentation of Antrodia camphorata S-29.
BACKGROUND
Antroquinonol have significantly anti-tumour effects on various cancer cells. There is still lack of reports on regulation of environmental factors on antroquinonol production by Antrodia camphorata.


RESULTS
An effective submerged fermentation method was employed to induce antroquinonol with adding H2O2. The production of antroquinonol was 57.81 mg L-1after fermentation for 10 days when adding 25 mmol L-1H2O2at day 4 of the fermentation process. Then, antroquinonol was further increased to 80.10 mg L-1with cell productivity of 14.94 mg g-1dry mycelium when the feeding rate of H2O2was adjusted to 0.2 mmol L-1h-1in the 7 L fermentation bioreactor. After inhibiting the generation of reactive oxygen species with the inhibitor diphenyleneiodoium, the synthesis of antroquinonol from A. camphorata was significantly reduced, and the yield was only 3.3 mg L-1.


CONCLUSIONS
The results demonstrated that addition of H2O2was a very effective strategy to induce and regulate the synthesis of antroquinonol in submerged fermentation. Reactive oxygen species generated by H2O2during fermentation caused oxidative stress, which induced the synthesis of antroquinonol and other chemical compounds. Moreover, it is very beneficial process to improve production and diversity of the active compounds during liquid fermentation of A. camphorata mycelium. © 2016 Society of Chemical Industry.
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Yongjun
Y
Xia
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Xuan
X
Zhou
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Guangqiang
G
Wang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Bobo
B
Zhang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Ganrong
G
Xu
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Lianzhong
L
Ai
D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D055491:Antrodia / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D019149:Bioreactors; D002681:China; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D009172:Mycology / Q000379:methods; D009861:Onium Compounds / Q000494:pharmacology; D009994:Osmolar Concentration; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D013237:Stereoisomerism; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D009861:Onium Compounds; D016877:Oxidants; D017382:Reactive Oxygen Species; C000620095:antroquinonol B; D014451:Ubiquinone; C007517:diphenyleneiodonium; C545357:antroquinonol; D006861:Hydrogen Peroxide
Antrodia camphorata; antroquinonol; hydrogen peroxide; induce; reactive oxygen species; submerged fermentation