Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt.
A novel halophilic archaeon designated strain CBA1114Twas isolated from solar salt in the Republic of Korea. Strain CBA1114T, cells of which were coccoid and Gram-stain-negative, grew in the presence of 15-30 % (w/v) NaCl (optimum, 20 %) and at 20-50 °C (optimum, 40 °C) and pH 7.0-9.0 (optimum, pH 8.0). Strain CBA1114Trequired Mg2+for growth. Strain CBA1114Thad three 16S rRNA genes, rrnA, rrnB and rrnC; levels of similarity between the sequences were 99.7-99.9 %. The 16S rRNA gene sequence of strain CBA1114Tshowed 91.7 % similarity to that of Haloterrigena thermotolerans PR5T. In multilocus sequence analysis (MLSA), five housekeeping genes, atpB, EF-2, radA, rpoB' and secY, were found to be closely related to those of the members of the genera Halorientalis(89.7 % similarity of the atpB gene sequence), Halomicroarcula(91.9 %, EF-2), Haloterrigena(85.4 %, radA), Natronoarchaeum(89.2 %, rpoB') and Natrinema(75.7 %, secY). A phylogenetic tree generated from the results of MLSA of the five housekeeping genes showed that strain CBA1114Twas closely related to species of the genus Halorientalis in the family Halobacteriaceae. The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and unidentified lipids. The G+C content of the genomic DNA of strain CBA1114Twas 68.1 mol%. According to the results of phylogenetic, phenotypic and chemotaxonomic analyses, we designate strain CBA1114T(=JCM 30111T=KCTC 4206T) as the type strain of Halostella salina gen. nov., sp. nov., a novel species of a new genus within the family Halobacteriaceae.
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, South Korea.|Department of Food Science and Engineering, Ewha Womans University, Seoul 03760, South Korea.
Hye Seon
HS
Song
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, South Korea.|Division of Bioengineering, Incheon National University, Incheon 22012, South Korea.
In-Tae
IT
Cha
Department of Food Science and Engineering, Ewha Womans University, Seoul 03760, South Korea.
Jin-Kyu
JK
Rhee
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, South Korea.
Kyung June
KJ
Yim
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, South Korea.
Ah Yoon
AY
Kim
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, South Korea.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, South Korea.
Jong-Soon
JS
Choi
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, South Korea.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 34134, South Korea.
Su Jeong
SJ
Baek
Division of Bioengineering, Incheon National University, Incheon 22012, South Korea.
Myung-Ji
MJ
Seo
Department of Biology, Jeju National University, Jeju 63243, South Korea.
Soo-Je
SJ
Park
Research Group of Gut Microbiome, Korea Food Research Institute, Sungnam 13539, South Korea.
Young-Do
YD
Nam
Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, South Korea.
Seong Woon
SW
Roh
D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D019766:Genes, Archaeal; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D058885:Multilocus Sequence Typing; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D019641:DNA, Archaeal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
Halobacteriales; Halostella salina; haloarchaea; polyphasic taxonomy; solar salt