The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica.
Mannheimia haemolytica is the most important bacterial pathogen isolated from cases of Bovine Respiratory Disease (BRD). Routine identification of these bacteria is usually performed using phenotypic methods. Our study showed that MALDI-TOF MS is a reliable alternative to these methods. All of the strains analyzed were identified as M. haemolytica. The identification results were compared to those obtained using conventional methods commonly used in microbiological diagnostics, based on detection and analysis of biochemical properties of microorganisms. The degree of agreement between the two methods for identifying M. haemolytica was 100%.
Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.
Andrzej
A
Puchalski
Renata
R
Urban-Chmiel
Marta
M
Dec
Diana
D
Stęgierska
Andrzej
A
Wernicki
D000818:Animals; D002417:Cattle; D016978:Mannheimia haemolytica; D048089:Pneumonia of Calves, Enzootic / Q000175:diagnosis* / Q000382:microbiology; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000662:veterinary*