Bio-statistical enhancement of acyl transfer activity of amidase for biotransformation of N-substituted aromatic amides.
Acyl transfer activity (ATA) of amidase transfers an acyl group of different amides to hydroxylamine to form the corresponding hydroxamic acid. Bacterial isolate BR-1 was isolated from cyanogenic plant Cirsium vulgare rhizosphere and identified as Pseudomonas putida BR-1 by 16S rDNA sequencing. This organism exhibited high ATA for the biotransformation of N-substituted aromatic amide to the corresponding hydroxamic acid. Optimization of media, tryptone (0.6%), inducer, pH 8.5, and a growth temperature 25°C for 56 h, resulted in a 7-fold increase in ATA. Further, Response Surface Methodology (RSM) and multiple feeding approach (20 mM after 14 h) of inducer led to a 29% enhancement of ATA from this organism. The half life (t1/2) of this enzyme at 50°C and 60°C was 3 h and 1 h, respectively. The ATA of amidase of Pseudomonas putida BR-1 makes it a potential candidate for the production of a variety of N-substituted aromatic hydroxamic acid.
Department of Biotechnology, Himachal Pradesh University.
Ravi Kant
RK
Bhatia
Shashi Kant
SK
Bhatia
Praveen Kumar
PK
Mehta
Tek Chand
TC
Bhalla
D000577:Amides / Q000378:metabolism*; D000581:Amidohydrolases / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D031180:Cirsium / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000378:metabolism*; D019811:Hydroxylamine / Q000737:chemistry / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D058441:Rhizosphere; D013379:Substrate Specificity; D013696:Temperature
D000577:Amides; D003470:Culture Media; D006877:Hydroxamic Acids; D019811:Hydroxylamine; D000581:Amidohydrolases; C019665:amidase