Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle.
Blastocyst complementation (BC) systems have enabled in vivo generation of organs from allogeneic pluripotent cells, compensating for an empty germ cell niche in gene knockout (KO) animals. Here, we succeeded in producing chimeric beef cattle (Wagyu) by transferring allogenic germ cells into ovaries using somatic cell nuclear transfer and BC technology. The KO of NANOS3 (NANOS3(-/-)) in Wagyu bovine ovaries produced a complete loss of germ cells. Holstein blastomeres (NANOS3(+/+)) were injected into NANOS3(-/-) Wagyu embryos. Subsequently, exogenous germ cells (NANOS3(+/+)) were identified in the NANOS3(-/-) ovary. These results clearly indicate that allogeneic germ cells can be generated in recipient germ cell-free gonads using cloning and BC technologies.
Research and Development Group, Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido 080-1407, Japan.
Atsushi
A
Ideta
Biological Sciences Section, Central Research Institute for Feed and Livestock of Zen-noh, Tsukuba, Ibaraki 300-4204, Japan.
Shiro
S
Yamashita
Research and Development Group, Zen-noh Institute of Animal Health, Sakura, Chiba 285-0043, Japan.
Marie
M
Seki-Soma
Research and Development Group, Zen-noh Institute of Animal Health, Sakura, Chiba 285-0043, Japan.
Ryosaku
R
Yamaguchi
Research and Development Group, Zen-noh Institute of Animal Health, Sakura, Chiba 285-0043, Japan.
Shiori
S
Chiba
Research and Development Group, Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido 080-1407, Japan.
Haruna
H
Komaki
Pig Breeding Laboratory, Central Research Institute for Feed and Livestock of Zen-noh, Kamishihoro, Hokkaido 080-1406, Japan.
Tetsuya
T
Ito
Research and Development Group, Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido 080-1407, Japan.
Masato
M
Konishi
Research and Development Group, Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido 080-1407, Japan.
Yoshito
Y
Aoyagi
Biological Sciences Section, Central Research Institute for Feed and Livestock of Zen-noh, Tsukuba, Ibaraki 300-4204, Japan.
Yutaka
Y
Sendai
D000818:Animals; D001757:Blastomeres / Q000502:physiology; D002417:Cattle; D002454:Cell Differentiation; D005260:Female; D055786:Gene Knockout Techniques; D005854:Germ Cells / Q000502:physiology*; D010053:Ovary / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000235:genetics*
D016601:RNA-Binding Proteins