Saikosaponin A, an active glycoside from Radix bupleuri, reverses P-glycoprotein-mediated multidrug resistance in MCF-7/ADR cells and HepG2/ADM cells.
1. The expression and function of P-glycoprotein (P-gp) is associated with the phenotype of multidrug resistance (MDR). Saikosaponin A (SSA) is a triterpenoid saponin isolated from Radix Bupleuri. This study was mainly designed to understand effects of SSA on MDR in MCF-7/ADR and HepG2/ADM cells. 2. MDR reversal was examined as the alteration of cytotoxic drugs IC50 in resistant cells in the presence of SSA by MTT assay, and was compared with the non-resistant cells. Apoptosis and uptake of P-gp substrates in the tumor cells were detected by flow cytometry. Western blot was performed to assay the expression of P-gp. 3. Our results demonstrate SSA could increase the chemosensitivity of P-gp overexpressing HepG2/ADM and MCF-7/ADR cells to doxorubicin (DOX), vincristine (VCR) and paclitaxel. SSA promoted apoptosis of MCF-7/ADR cells in the presence of DOX. Moreover, it could also increase the retention of P-gp substrates DOX and rhodamine 123 in MCF-7/ADR cells, and decrease digoxin efflux ratio in Caco-2 cell monolayer. Finally, a mechanistic study showed that SSA reduced P-gp expression without affecting hydrolytic activity of P-gp. 4. In conclusion, our findings suggest that SSA could be further developed for sensitizing resistant cancer cells and used as an adjuvant therapy together with anticancer drugs to improve their therapeutic efficacies.
a Department of Medical Oncology , The Second Affiliated Hospital of Anhui Medical University , Hefei , China.
Rui-Ping
RP
Ye
a Department of Medical Oncology , The Second Affiliated Hospital of Anhui Medical University , Hefei , China.
Zhen-Dong
ZD
Chen
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple / Q000235:genetics*; D056945:Hep G2 Cells; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*
D018435:ATP Binding Cassette Transporter, Subfamily B; D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D
HepG2/ADM; MCF-7/ADR; P-glycoprotein; Saikosaponin A; multidrug resistance