A Stimuli-Responsive Biosensor of Glucose on Layer-by-Layer Films Assembled through Specific Lectin-Glycoenzyme Recognition.
The research on intelligent bioelectrocatalysis based on stimuli-responsive materials or interfaces is of great significance for biosensors and other bioelectronic devices. In the present work, lectin protein concanavalin A (Con A) and glycoenzyme glucose oxidase (GOD) were assembled into {Con A/GOD}n layer-by-layer (LbL) films by taking advantage of the biospecific lectin-glycoenzyme affinity between them. These film electrodes possess stimuli-responsive properties toward electroactive probes such as ferrocenedicarboxylic acid (Fc(COOH)₂) by modulating the surrounding pH. The CV peak currents of Fc(COOH)₂ were quite large at pH 4.0 but significantly suppressed at pH 8.0, demonstrating reversible stimuli-responsive on-off behavior. The mechanism of stimuli-responsive property of the films was explored by comparative experiments and attributed to the different electrostatic interaction between the films and the probes at different pH. This stimuli-responsive films could be used to realize active/inactive electrocatalytic oxidation of glucose by GOD in the films and mediated by Fc(COOH)₂ in solution, which may establish a foundation for fabricating novel stimuli-responsive electrochemical biosensors based on bioelectrocatalysis with immobilized enzymes.
Department of Pharmacy, Ningxia Medical University, Yinchuan 750004, China. huiqin_yao@163.com.
Huiqin
H
Yao
Department of Pharmacy, Ningxia Medical University, Yinchuan 750004, China. sarah_ganq@163.com.
Qianqian
Q
Gan
State Key Laboratory Cultivation Base of Natural Gas Conversion, Ningxia University, Yinchuan 750021, China. pengjuan@nxu.edu.cn.|School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China. pengjuan@nxu.edu.cn.
Juan
J
Peng
Department of Pharmacy, Ningxia Medical University, Yinchuan 750004, China. jay70281@163.com.
Shan
S
Huang
Department of Pharmacy, Ningxia Medical University, Yinchuan 750004, China. zhanggb@nxmu.edu.cn.
Meilin
M
Zhu
State Key Laboratory Cultivation Base of Natural Gas Conversion, Ningxia University, Yinchuan 750021, China. shikeren@nxu.edu.cn.
Keren
K
Shi
D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification*; D005949:Glucose Oxidase / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000075163:Metallocenes; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry
D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D011108:Polymers; D003208:Concanavalin A; C032949:ferrocenecarboxylic acid; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose
concanavalin A; enzyme immobilization; ferrocenedicarboxylic acid; layer-by-layer assembly; lectin-sugar specific interaction; stimuli-responsive biosensor