Allelic loss at PTEN locus leads to progression of colorectal carcinoma among North Indian patients.
We evaluated the loss of heterozygosity (LOH) at 10q23.3locus of microsatellite markers; D10S198, D10S192, and D10S541 of PTEN gene in 223 North Indian colorectal cancer (CRC) specimens. DNA was isolated and microsatellite-specific markers polymerase chain reaction was performed. Out of total 223 cases 102 showed LOH for at least one of the locus. In addition, thereto a significant association was found with the clinicopathologic features like grade of differentiation, clinical stage, invasion, lymph node invasion, and the clinical outcome (p < 0.05). These data argue that the given markers to check the possible LOH of PTEN gene at locus 10q23.3could be considered as one of the diagnostic markers in CRC.
a Department of Biochemistry , AIIMS , Patna , India.
Asgar
A
Ali
b Department of Biosciences, Genome Biology Lab , Jamia Millia Islamia , New Delhi , India.
Syed Jafar
SJ
Mehdi
c School of Life Sciences , Devi Ahilya Vishwavidyalaya , Indore , India.
Krishnan
K
Hajela
d Department of Gastrointestinal Surgery , G. B. Pant Hospital , New Delhi , India.
Sundeep Singh
SS
Saluja
d Department of Gastrointestinal Surgery , G. B. Pant Hospital , New Delhi , India.
Pramod Kumar
PK
Mishra
e Basic Medical Sciences, College of Medicine-Jeddah, King Saud bin Abdulaziz University for Health Sciences , Riyadh , Saudi Arabia.
Aga Syed
AS
Sameer
b Department of Biosciences, Genome Biology Lab , Jamia Millia Islamia , New Delhi , India.
M Moshahid Alam
MMA
Rizvi
Colorectal cancer; PCR; PTEN; loss of heterozygosity; microsatellite marker