Identification of MBF2 family genes in Bombyx mori and their expression in different tissues and stages and in response to Bacillus bombysepticus infection and starvation.
The Multiprotein bridge factor 2 (MBF2) gene was first identified as a co-activator involved in BmFTZ-F1-mediated activation of the Fushi tarazu gene. Herein, nine homologous genes of MBF2 gene are identified. Evolutionary analysis showed that this gene family is insect-specific and that the family members are closely related to response to pathogens (REPAT) genes. Tissue distribution analysis revealed that these genes could be expressed in a tissue-specific manner. Developmental profiles analysis showed that the MBF2 gene family members were highly expressed in the different stages. Analysis of the expression patterns of nine MBF2 family genes showed that Bacillus bombysepticus treatment induced the up-regulation of several MBF2 family genes, including MBF2-4, -7, -9, -8. Furthermore, we found the MBF2 family genes were modulated by starvation and the expression of these genes recovered upon re-feeding, except for MBF2-5, -9. These findings suggested roles for these proteins in insect defense against pathogens and nutrient metabolism, which has an important guiding significance for designing pest control strategies.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Chun-Yan
CY
Zhou
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Xing-Fu
XF
Zha
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Chun
C
Liu
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Min-Jin
MJ
Han
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Li-Ying
LY
Zhang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Pan-Pan
PP
Shi
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
He
H
Wang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Ren-Wen
RW
Zheng
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Qing-You
QY
Xia
D000818:Animals; D001407:Bacillus / Q000502:physiology*; D012831:Bombyx / Q000235:genetics* / Q000382:microbiology* / Q000502:physiology; D005508:Food Deprivation; D051717:Fushi Tarazu Transcription Factors / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D010802:Phylogeny
D051717:Fushi Tarazu Transcription Factors; D019476:Insect Proteins
immunity; nutrient metabolism; physiology; whole-genome scanning