Paludibacterium purpuratum sp. nov., isolated from wetland soil.
A novel bacterium, designated KJ031T, was isolated from a wetland soil sample taken from Jeju island, Republic of Korea. Cells were Gram-stain-negative, curved rod shaped, oxidase- and catalase- positive, motile and facultatively anaerobic. Growth was observed at pH 6.0-8.0 and at 20-37 °C on R2A agar. Comparative analysis of 16S rRNA gene sequences revealed that strain KJ031Tis a member of the genus Paludibacterium, sharing highest sequence similarities with Paludibacterium paludis KBP-21T(96.2 %) and Paludibacterium. yongneupense 5YN8-15T(96.0 %). The major fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The predominant respiratory quinone was Q-8. The major polar lipids of strain KJ031Twere diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, two unidentified phospholipids and one unidentified polar lipid. The DNA G+C content was 59.2 mol%. On the basis of the evidence presented in this study, strain KJ031Trepresents a novel species of the genus Paludibacterium, for which the name Paludibacterium purpuratum sp. nov. is proposed. The type strain is KJ031T(=KCTC 42852T=CECT 8976T).
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, South Korea.
Heeyoung
H
Kang
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, South Korea.
Haneul
H
Kim
Department of Biological Sciences, Inha University, Incheon 22212, South Korea.
Yochan
Y
Joung
Department of Biology, Gangneung-Wonju National University, Gangwon 25457, South Korea.
Kyu-Joong
KJ
Kim
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, South Korea.
Kiseong
K
Joh
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry; D053833:Wetlands
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8
16S rRNA gene; Paludibacterium purpuratum sp. nov.; wetland