Antimicrobial effect of three disinfecting agents on Resilon cones and their effect on surface topography: An in vitro study.
OBJECTIVE
The objective of this study was to evaluate the effectiveness of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and 2% peracetic acid (PAA) in disinfecting Resilon cones and to evaluate topographical changes microscopically under scanning electron microscope (SEM) after rapid chemical disinfection.


METHODS
Resilon cones were disinfected in an ultraviolet (UV) light chamber for 20 min and contaminated by immersing in a microbial suspension of Enterococcus faecalis for 30 min. The contaminated cones were then immersed in the 5.25% NaOCl, 2% CHX, and 2% PAA for 1 min, 5 min, and 10 min, separately. The cones were then incubated at 37°C in thioglycollate broth for 7 days and examined for turbidity. The samples showing turbidity were subcultured on blood agar and incubated at 37°C for 48 h. Gram staining was done to confirm that the cultured bacteria were E. faecalis. Surface changes of disinfected Resilon cones were evaluated under SEM.


METHODS
The data were analyzed statistically using Kruskal-Wallis, analysis of variance (ANOVA), and Mann-Whitney U-test.


RESULTS
In eliminating E. faecalis, 5.25% NaOCl was most effective followed by 2% PAA and 2% CHX. Topographic examination of tested Resilon cones revealed some surface deposits after disinfection with 5.25% NaOCl and 2% CHX, whereas 2% PAA caused surface erosion.


CONCLUSIONS
In disinfecting Resilon cones, 5.25% NaOCl is most effective followed by 2% PAA and 2% CHX.
Department of Conservative Dentistry and Endodontics, AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, Karnataka, India.
Mahesh Martur
MM
Chandrappa
Department of Conservative Dentistry and Endodontics, AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, Karnataka, India.
Prasanna Mahadevasa
PM
Meharwade
Department of Conservative Dentistry and Endodontics, AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, Karnataka, India.
Raghu
R
Srinivasan
Department of Conservative Dentistry and Endodontics, AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, Karnataka, India.
Shreetha
S
Bhandary
Department of Conservative Dentistry and Endodontics, AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, Karnataka, India.
Farhat
F
Nasreen
Disinfection; Resilon; peracetic acid (PAA)