Generation of siRNA Nanosheets for Efficient RNA Interference.
After the discovery of small interference RNA (siRNA), nanostructured siRNA delivery systems have been introduced to achieve an efficient regulation of the target gene expression. Here we report a new siRNA-generating two dimensional nanostructure in a formation of nanosized sheet. Inspired by tunable mechanical and functional properties of the previously reported RNA membrane, siRNA nanosized sheets (siRNA-NS) with multiple Dicer cleavage sites were prepared. The siRNA-NS has two dimensional structure, providing a large surface area for Dicer to cleave the siRNA-NS for the generation of functional siRNAs. Furthermore, downregulation of the cellular target gene expression was achieved by delivery of siRNA-NS without chemical modification of RNA strands or conjugation to other substances.
Department of Chemical Engineering, University of Seoul, Seoul 02504, Korea.
Hyejin
H
Kim
Department of Chemical Engineering, University of Seoul, Seoul 02504, Korea.
Jae Sung
JS
Lee
Department of Chemical Engineering, University of Seoul, Seoul 02504, Korea.
Jong Bum
JB
Lee
D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D058990:Molecular Targeted Therapy / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D000067492:RNAi Therapeutics / Q000379:methods*
D034741:RNA, Small Interfering