Fruit Extract from Pyropolyporus fomentarius (L. ex Fr.) Teng Induces Mitochondria-Dependent Apoptosis in Leukemia Cells but Enhances Immunomodulatory Activities of Splenic Lymphocytes.
Pyropolyporus fomentarius (L. ex Fr.) Teng is a unique woody mushroom due to its medicinal value with numerous pharmacological activities. This study presented the potential antitumor and immunomodulatory properties of ethanol extract of P. fomentarius. The results showed that P. fomentarius extract inhibited the viability of murine leukemia L1210 cells in a dose-dependent manner with IC50 value of 69.35 μg/ml. Flow cytometry analysis demonstrated that the extract induced apoptosis in L1210 cells. Additionally, the decline of mitochondrial membrane potential was observed as well as the changes of caspase-3, caspase-9, Bcl-2, and Bax, suggesting that proapoptosis effect of the extract involved mitochondria-related pathway. Simultaneously, the P. fomentarius extract significantly enhanced the proliferation and activation of splenic lymphocytes in a dose-dependent manner. This P. fomentarius extract has potential applications as a natural antitumor agent with immunomodulatory activity.
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Yixiang
Y
Li
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Yuetao
Y
Zhou
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Xiaobing
X
Wang
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Pan
P
Wang
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Yaping
Y
Xiao
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Xiaoxia
X
Cheng
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Yanhua
Y
Zhang
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Yilin
Y
Liu
a Key Laboratory of the Ministry of Education for Medicinal Mushroom Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University , Xi'an , China.
Quanhong
Q
Liu
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D007939:Leukemia L1210; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects; D011124:Polyporaceae / Q000737:chemistry*; D013154:Spleen / Q000166:cytology
D000970:Antineoplastic Agents; D007155:Immunologic Factors