The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implantation time.
OBJECTIVE
Progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. It is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time.


METHODS
Adult female NMRI mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimulation]. Uterine horn samples removed at pre-implantation time in each group. Motic image Plus 2 software was used to assess the quantitative vascular parameters of endometrium. Gene expression was determined for vascular endothelial growth factor (VEGF), FMS-like tyrosine kinase (FLT) and Kinase insert domain protein receptor (FLK) genes using the real time PCR method. Data analysis was done with LinReg PCR and Rest-RG software.


RESULTS
Comparison between progestrone treated mice after ovarian stimulation with control group showed that increase in rate of VEGF gene expression [0.775] and decrease in rate of FLK [6.072] and FLT [1.711] gene expression. Analysis of the data on quantitative vascular parameters were indicated remarkable increase in quantitative vascular parameters of progestrone treated mice compare to control group.


CONCLUSIONS
Biological effect of progestrone on the vascular changes after ovarian stimulation resulted in an increase in VEGF receptors experession, it seems that induced angiogenesis by progesterone could result in better condition for implantation.
Deptartment of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.
Mandana Beigi
MB
Boroujeni
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Nasim Beigi
NB
Boroujeni
Deptartment of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.
Mohammadreza
M
Gholami
Angiogenesis; Endometrium; Gene expression; Pre-implantation time; Progesterone; Vascular parameter