Fabrication of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays for enhancing bactericidal effect and osteoinductivity.
Implant-associated infection is a common postoperative complication and remains a serious problem in orthopedic surgery. This work describes the synthesis of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays (AgNP-BHAC). The resulting nanostructure was investigated using scanning electron microscopy, energy-dispersive spectrometry, transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy. AgNP-BHAC exhibited excellent antimicrobial activity toward gram-negative Escherichia coli and gram-positive Staphylococcus aureus owing to the antibacterial effects of the silver nanoparticles. Human bone marrow stromal cells (hBMSC) culture revealed that the AgNP-BHAC exhibited better biocompatibility, and permitted improved cell proliferation, attachment, and osteoinductivity than uncoated Ti-6Al-4V titanium alloy, the favored material for biomedical applications. In summary, this study presents a convenient and effective method for the incorporation of silver into HA coatings with block morphology. This method can be utilized to modify a variety of metallic implant surfaces to improve their antimicrobial effects and reduce potential long-term cytotoxicity.
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People׳s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Bo
B
Tian
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
Wei
W
Chen
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People׳s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Degang
D
Yu
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
Yong
Y
Lei
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
Qinfei
Q
Ke
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China. Electronic address: ypguo@shnu.edu.cn.
Yaping
Y
Guo
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People׳s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. Electronic address: zhuzhenan2006@126.com.
Zhenan
Z
Zhu
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus; D017154:Stromal Cells / Q000166:cytology; D014025:Titanium; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D012834:Silver; D017886:Durapatite; D014025:Titanium
Antibacterial property; Hydroxyapatite coating; Osteogenic; Silver nanoparticle