Quantitative determination of 15 bioactive triterpenoid saponins in different parts of Acanthopanax henryi by HPLC with charged aerosol detection and confirmation by LC-ESI-TOF-MS.
Triterpenoid saponins are difficult to analyze using high-performance liquid chromatography coupled to UV/vis spectrophotometry due to their lack of chromophores. This study describes the first analytical method for the determination of 15 triterpenoid saponins from the leaves, stems, root bark, and fruits of Acanthopanax henryi, using a high-performance liquid chromatography with charged aerosol detection coupled with electrospray ionization mass spectrometry method. The separation was carried out on a Kinetex XB-C18 column with an acetonitrile/water gradient as the mobile phase, followed by charged aerosol detection. The operating conditions of charged aerosol detection were set at 24 kPa for nitrogen pressure and 100 pA for the detection range. Liquid chromatography with electrospray ionization mass spectrometry is described for the identification of compounds in plant samples. The electrospray ionization mass spectrometry method involved the use of the [M + Na](+) and [M + NH4 ](+) ions for compounds 1-15 in the positive ion mode with an extracted ion chromatogram. The developed method was fully validated in terms of linearity, sensitivity, precision, repeatability, and recovery, then subsequently applied to evaluate the quality of A. henryi.
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
Xiao-Dan
XD
Zhang
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
Zhi
Z
Li
Guangdong Drug Research Institute of Dongyangguang Group, Dongguan, China.
Guo-Zhu
GZ
Liu
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
Xiang
X
Wang
Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Korea.
Ok-Kyoung
OK
Kwon
Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Korea.
Hyeong-Kyu
HK
Lee
Pharmaceutical Resources Botany Laboratory, Chung-Ang University, Seoul, Korea.
Wan-Kyunn
WK
Whang
School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
Xiang-Qian
XQ
Liu
D000336:Aerosols / Q000032:analysis*; D002853:Chromatography, Liquid; D028001:Eleutherococcus / Q000737:chemistry*; D012503:Saponins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000032:analysis*
D000336:Aerosols; D012503:Saponins; D014315:Triterpenes
Acanthopanax henryi; Charged aerosol detection; Quality control; Triterpenoid saponins