Interactions of lauryl gallate with phospholipid components of biological membranes.
The effect of different amounts of lauryl gallate (LG) on properties of the model membranes of phosphatidylcholines (PC), differing in the presence of double bonds in the hydrocarbon chains, and phosphatidylglycerol (PG) was described in terms of phase behaviour of mixtures, interactions between both components, monolayers stability and their organization. The Langmuir monolayer technique was used to monitor the surface thermodynamics (i.e. the excess area and excess Gibbs energy of mixing) on the basis of surface pressure-area per molecule (π-A) isotherms. Simultaneously, morphology of the studied monolayers was visualized by the Brewster angle microscopy (BAM). This allowed evaluating the kind and magnitude of interactions which influence on the phase behaviour and structural properties of the monolayers. The obtained results can be helpful to reveal the mechanism of phospholipid antioxidant protection and important pharmacological (antimicrobial) role of lauryl gallate for production of effective therapeutic substances.
Department of Physical Chemistry-Interfacial Phenomena, Faculty of Chemistry, University of Maria Curie-Skłodowska, Maria Curie-Skłodowska Sq. 3, 20031 Lublin, Poland. Electronic address: malgorzata.jurak@poczta.umcs.lublin.pl.
Małgorzata
M
Jurak
Department of Physical Chemistry, Faculty of Pharmacy, University of Santiago de Compostela, Campus Sur, 15705 Santiago de Compostela, Spain.
José
J
Miñones
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D008563:Membrane Lipids / Q000737:chemistry*; D008859:Microscopy, Polarization; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology
D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D013501:Surface-Active Agents; D015060:1,2-Dipalmitoylphosphatidylcholine; C008259:lauryl gallate; D005707:Gallic Acid; C017251:1,2-oleoylphosphatidylcholine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine
Brewster angle microscopy; Langmuir monolayer; Lauryl gallate; Miscibility; Phospholipid