Assessment of developmental cardiotoxic effects of some commonly used phytochemicals in mouse embryonic D3 stem cell differentiation and chick embryonic cardiomyocyte micromass culture models.
Pregnant women often use herbal medicines to alleviate symptoms of pregnancy. The active phytochemicals eugenol (from holy basil) and α-bisabolol (from chamomile) are recommended to promote calmness and reduce stress. There is evidence that both eugenol and α-bisabolol possess pro-apoptotic and anti-proliferative effects and induce reactive oxygen species. The potential effect was examined by monitoring cardiomyocyte contractile activity (differentiation), cell activity, protein content and ROS production for mouse D3 embryonic stem cell and ‎chick embryonic micromass culture. The results showed that eugenol (0.01-80μM) demonstrated effects on cell activity (both systems) and ROS production (stem cell system only), as well as decreasing the contractile activity and protein content at high concentrations in both systems. Additionally, α-bisabolol (0.01-80μM) at high concentrations decreased the contractile activity and cell activity and in the stem cell system induced ROS production and decreased protein content. The results suggest only low concentrations should be ingested in pregnancy.‎.
School of Life Sciences, Faculty of Medicine and Health Sciences, Medical School, QMC, University of Nottingham, Nottingham NG7 2UH, United Kingdom.
Omar J
OJ
Mohammed
School of Life Sciences, Faculty of Medicine and Health Sciences, Medical School, QMC, University of Nottingham, Nottingham NG7 2UH, United Kingdom.
Roseanna
R
McAlpine
School of Life Sciences, Faculty of Medicine and Health Sciences, Medical School, QMC, University of Nottingham, Nottingham NG7 2UH, United Kingdom.
Phasawee
P
Chiewhatpong
School of Life Sciences, Faculty of Medicine and Health Sciences, Medical School, QMC, University of Nottingham, Nottingham NG7 2UH, United Kingdom.
Muhammad Liaque
ML
Latif
School of Life Sciences, Faculty of Medicine and Health Sciences, Medical School, QMC, University of Nottingham, Nottingham NG7 2UH, United Kingdom. Electronic address: margaret.pratten@nottingham.ac.uk.
Margaret K
MK
Pratten
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D005054:Eugenol / Q000633:toxicity*; D051379:Mice; D000081222:Monocyclic Sesquiterpenes; D000066450:Mouse Embryonic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D028321:Plant Preparations / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012717:Sesquiterpenes / Q000633:toxicity*
D000081222:Monocyclic Sesquiterpenes; D028321:Plant Preparations; D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; C004497:bisabolol; D005054:Eugenol
Chick embryonic cardiomyocytes; Embryotoxicity; Eugenol; Mouse D3 embryonic stem cells; α-Bisabolol