Laser-Based Methods for Detection of Nitric Oxide in Plants.
Nitric oxide (NO) plays an important role in plant signaling and in response to various stress conditions. Therefore, real-time measurements of NO production provide better insights into understanding plant processes and can help developing strategies to improve food production and postharvest quality. Using laser-based spectroscopic methods, sensitive, online, in planta measurements of plant-pathogen interactions are possible. This chapter introduces the basic principle of the optical detectors using different laser sources for accurate monitoring of fast dynamic changes of NO production. Several applications are also presented to demonstrate the suitability of these detectors for detection of NO in plants.
Department of Molecular and Laser Physics, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
Julien
J
Mandon
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais Campus, Edward Llywd Building, Aberystwyth, Wales, SY23 3DA, UK.
Luis A J
LA
Mur
Department of Molecular and Laser Physics, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
Frans J M
FJ
Harren
Department of Molecular and Laser Physics, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands. s.cristescu@science.ru.nl.
Simona M
SM
Cristescu
D054884:Host-Pathogen Interactions; D009569:Nitric Oxide / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D011789:Quantum Theory
D009569:Nitric Oxide
Laser; Nitric oxide; Optical detection; Plant-pathogen interaction