Flow cytometry crossmatch reactivity with pronase-treated T cells induced by non-HLA autoantibodies in human immunodeficiency virus-infected patients.
Pronase treatment is used in the flow cytometry crossmatch (FCXM) to prevent nonspecific antibody binding on B cells. However, we have observed unexpected positive results with pronase-treated T cells in human immunodeficiency virus (HIV)-infected patients. In this study, 25 HIV-infected patients without HLA antibodies were tested with pronase-treated and nontreated cells. HIV-positive sera were pretreated with reducing agents and preabsorbed with pronase-treated and nontreated T or B cells before crossmatching. All patients displayed FCXM reactivity with pronase-treated T cells but not with nontreated T cells. None of the patients exhibited FCXM reactivity with pronase-treated and nontreated B cells. These patients displayed FCXM reactivity with pronase-treated CD4+ and CD8+ T cells but not with their nontreated counterparts. Preabsorption with pronase-treated T cells reduced the T cell FCXM reactivity. Preabsorption with pronase-treated B cells or nontreated T and B cells did not have any effect on the T cell FCXM reactivity. Pretreatment with reducing agents did not affect the T cell FCXM reactivity. 15 of 21 HIV-infected kidney allograft recipients with pronase-treated T cell FCXM reactivity display long-term graft survival (1193±631days). These data indicate that HIV-infected patients have nondeleterious autoantibodies recognizing cryptic epitopes exposed by pronase on T cells.
Gift of Hope Organ & Tissue Donor Network, Itasca, IL 60143, USA; Department of Laboratory Medicine and Pathology, Mayo Clinic Hospital, Phoenix, AZ 85054, USA.
Katarzyna
K
Szewczyk
Gift of Hope Organ & Tissue Donor Network, Itasca, IL 60143, USA.
Kelly
K
Barrios
Gift of Hope Organ & Tissue Donor Network, Itasca, IL 60143, USA.
Daniel
D
Magas
Gift of Hope Organ & Tissue Donor Network, Itasca, IL 60143, USA.
Kristin
K
Sieg
Gift of Hope Organ & Tissue Donor Network, Itasca, IL 60143, USA.
Bozena
B
Labuda
Gift of Hope Organ & Tissue Donor Network, Itasca, IL 60143, USA.
Martin D
MD
Jendrisak
Gift of Hope Organ & Tissue Donor Network, Itasca, IL 60143, USA; Department of Laboratory Medicine and Pathology, Mayo Clinic Hospital, Phoenix, AZ 85054, USA. Electronic address: jaramillo.andres@mayo.edu.
Andrés
A
Jaramillo
D001323:Autoantibodies / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis / Q000517:prevention & control*; D006678:HIV / Q000502:physiology*; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D011402:Pronase / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology
D001323:Autoantibodies; D000939:Epitopes; D006680:HLA Antigens; D011402:Pronase
Autoantibodies; Flow Cytometry Crossmatch; Human Immunodeficiency Virus; Pronase