Identification and absolute quantification of enzymes in laundry detergents by liquid chromatography tandem mass spectrometry.
In a stricter legislative context, greener detergent formulations are developed. In this way, synthetic surfactants are frequently replaced by bio-sourced surfactants and/or used at lower concentrations in combination with enzymes. In this paper, a LC-MS/MS method was developed for the identification and quantification of enzymes in laundry detergents. Prior to the LC-MS/MS analyses, a specific sample preparation protocol was developed due to matrix complexity (high surfactant percentages). Then for each enzyme family mainly used in detergent formulations (protease, amylase, cellulase, and lipase), specific peptides were identified on a high resolution platform. A LC-MS/MS method was then developed in selected reaction monitoring (SRM) MS mode for the light and corresponding heavy peptides. The method was linear on the peptide concentration ranges 25-1000 ng/mL for protease, lipase, and cellulase; 50-1000 ng/mL for amylase; and 5-1000 ng/mL for cellulase in both water and laundry detergent matrices. The application of the developed analytical strategy to real commercial laundry detergents enabled enzyme identification and absolute quantification. For the first time, identification and absolute quantification of enzymes in laundry detergent was realized by LC-MS/MS in a single run. Graphical Abstract Identification and quantification of enzymes by LC-MS/MS.
Université de Lyon, Institut des Sciences Analytiques UMR 5280 CNRS, Université Claude Bernard Lyon 1, ENS Lyon, 5 rue de la Doua, 69100, Villeurbanne, France. alexandragaubert33@gmail.com.
Alexandra
A
Gaubert
Université de Lyon, Institut des Sciences Analytiques UMR 5280 CNRS, Université Claude Bernard Lyon 1, ENS Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.
Jérémy
J
Jeudy
Université de Lyon, Institut des Sciences Analytiques UMR 5280 CNRS, Université Claude Bernard Lyon 1, ENS Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.
Blandine
B
Rougemont
Université de Lyon, Institut des Sciences Analytiques UMR 5280 CNRS, Université Claude Bernard Lyon 1, ENS Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.
Claire
C
Bordes
Université de Lyon, Institut des Sciences Analytiques UMR 5280 CNRS, Université Claude Bernard Lyon 1, ENS Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.
Jérôme
J
Lemoine
Université de Lyon, Institut des Sciences Analytiques UMR 5280 CNRS, Université Claude Bernard Lyon 1, ENS Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.
Hervé
H
Casabianca
Université de Lyon, Institut des Sciences Analytiques UMR 5280 CNRS, Université Claude Bernard Lyon 1, ENS Lyon, 5 rue de la Doua, 69100, Villeurbanne, France. arnaud.salvador@univ-lyon1.fr.
Arnaud
A
Salvador
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D003902:Detergents / Q000737:chemistry*; D004798:Enzymes / Q000032:analysis*; D007846:Laundering; D057230:Limit of Detection; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
D003902:Detergents; D004798:Enzymes
Enzyme; Laundry detergent; Mass spectrometry; Protein; Sample preparation