Effects of cranberry components on IL-1β-stimulated production of IL-6, IL-8 and VEGF by human TMJ synovial fibroblasts.
OBJECTIVE
Osteoarthritis (OA) in the TMJ is characterized by deterioration of articular cartilage and secondary inflammatory changes. Interleukin-1β (IL-1β) stimulates IL-6, IL-8, and vascular endothelial growth factor (VEGF) in synovial fluid of TMJ with internal derangement and bony changes. The cranberry (Vaccinium macrocarpon) contains polyphenolic compounds that inhibit production of pro-inflammatory molecules by gingival cells in response to several stimulators. This study examined effects of cranberry components on IL-1β-stimulated IL-6, IL-8, and VEGF production by human TMJ synovial fibroblast-like cells.


METHODS
Cranberry high molecular weight non-dialyzable material (NDM) was derived from cranberry juice. Human TMJ synovial fibroblast-like cells from joints with degenerative OA and an ankylosed TMJ without degeneration were incubated with IL-1β (0.001-1nM)±NDM (25-250μg/ml) (2h preincubation). Viability was assessed via activity of a mitochondrial enzyme. IL-6, IL-8, and VEGF in culture supernatants were measured by ELISA; NF-κB and AP-1 transcription factors were measured in nuclear extracts via binding to specific oligonucleotides.


METHODS
ANOVA and Scheffe's F procedure for post hoc comparisons.


RESULTS
NDM did not affect cell viability but inhibited IL-1β stimulated IL-6, IL-8, and VEGF production in all cell lines (p<0.05). NDM partially reduced nuclear levels of NF-κB and AP-1 (p<0.04), depending upon cell line and time of exposure to IL-1β+NDM.


CONCLUSIONS
Cranberry NDM inhibition of IL-1β-stimulated IL- 6, IL-8, and VEGF production by TMJ synovial fibroblast-like cells suggests that cranberry components may be useful as a host modulatory therapeutic agent to prevent or treat inflammatory arthropathies of the TMJ.
College of Dentistry, The University of Tennessee Health Science Center, 62 South Dunlap Street, Memphis, TN 38163, USA; Department of Bioscience Research, The University of Tennessee Health Science Center, 62 South Dunlap Street, Memphis, TN 38163, USA. Electronic address: dtipton@uthsc.edu.
David A
DA
Tipton
College of Dentistry, The University of Tennessee Health Science Center, 62 South Dunlap Street, Memphis, TN 38163, USA; Department of Oral and Maxillofacial Surgery, The University of Tennessee Health Science Center, 62 South Dunlap Street, Memphis, TN 38163, USA. Electronic address: jchris19@uthsc.edu.
James
J
Christian
College of Dentistry, The University of Tennessee Health Science Center, 62 South Dunlap Street, Memphis, TN 38163, USA; Department of Oral and Maxillofacial Surgery, The University of Tennessee Health Science Center, 62 South Dunlap Street, Memphis, TN 38163, USA. Electronic address: info@omscarolina.com.
Adam
A
Blumer
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D013583:Synovial Membrane / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013704:Temporomandibular Joint / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029799:Vaccinium macrocarpon / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000093242:Interleukin-6 Inhibitors
D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D010936:Plant Extracts; D059808:Polyphenols; D042461:Vascular Endothelial Growth Factor A; D000093242:Interleukin-6 Inhibitors; C508606:CXCL8 protein, human; C508600:IL6 protein, human; C467484:VEGFA protein, human
Cranberry; Cytokine; IL-1β; Synovial fibroblast; TMJ