Recycling cellulase towards industrial application of enzyme treatment on hardwood kraft-based dissolving pulp.
Cost-effectiveness is vital for enzymatic treatment of dissolving pulp towards industrial application. The strategy of cellulase recycling with fresh cellulase addition was demonstrated in this work to activate the dissolving pulp, i.e. decreasing viscosity and increasing Fock reactivity. Results showed that 48.8-35.1% of cellulase activity can be recovered from the filtered liquor in five recycle rounds, which can be reused for enzymatic treatment of dissolving pulp. As a result, the recycling cellulase with addition fresh cellulase of 1mg/g led to the pulp of viscosity 470mL/g and Fock reactivity 80%, which is comparable with cellulase charge of 2mg/g. Other pulp properties such as alpha-cellulose, alkaline solubility and molecular weight distribution were also determined. Additionally, a zero-release of recycling cellulase treatment was proposed to integrate into the dissolving pulp production process.
Key Lab of Paper Science and Technology of Ministry of Education, Qilu University of Technology, Jinan, Shandong Province 250353, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, Guangdong Province 510640, China; Limerick Pulp and Paper Centre, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada. Electronic address: wangqiang8303@163.com.
Qiang
Q
Wang
Key Lab of Paper Science and Technology of Ministry of Education, Qilu University of Technology, Jinan, Shandong Province 250353, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, Guangdong Province 510640, China; Limerick Pulp and Paper Centre, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.
Shanshan
S
Liu
Key Lab of Paper Science and Technology of Ministry of Education, Qilu University of Technology, Jinan, Shandong Province 250353, China.
Guihua
G
Yang
Key Lab of Paper Science and Technology of Ministry of Education, Qilu University of Technology, Jinan, Shandong Province 250353, China.
Jiachuan
J
Chen
Key Lab of Paper Science and Technology of Ministry of Education, Qilu University of Technology, Jinan, Shandong Province 250353, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, Guangdong Province 510640, China.
Xingxiang
X
Ji
Limerick Pulp and Paper Centre, University of New Brunswick, Fredericton, New Brunswick E3B 5A3, Canada.
Yonghao
Y
Ni
D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D008970:Molecular Weight; D059027:Recycling / Q000379:methods*; D012995:Solubility; D014783:Viscosity; D014934:Wood / Q000737:chemistry*
D002482:Cellulose; D002480:Cellulase
Cellulase treatment; Cost effectiveness; Dissolving pulp; Recycling