Detection of DNA viruses in prostate cancer.
We tested prostatic secretions from men with and without prostate cancer (13 cases and 13 matched controls) or prostatitis (18 cases and 18 matched controls) with metagenomic sequencing. A large number (>200) of viral reads was only detected among four prostate cancer cases (1 patient each positive for Merkel cell polyomavirus, JC polyomavirus and Human Papillomavirus types 89 or 40, respectively). Lower numbers of reads from a large variety of viruses were detected in all patient groups. Our knowledge of the biology of the prostate may be furthered by the fact that DNA viruses are commonly shed from the prostate and can be readily detected by metagenomic sequencing of expressed prostate secretions.
International Agency for Research on Cancer, World Health Organization, Screening Group, Lyon, 69372, France.|Karolinska Institutet, Department of Laboratory Medicine, Stockholm, 141 86, Sweden.
Vitaly
V
Smelov
International Agency for Research on Cancer, World Health Organization, Screening Group, Lyon, 69372, France.
Davit
D
Bzhalava
International Agency for Research on Cancer, World Health Organization, Screening Group, Lyon, 69372, France.
Laila Sara
LS
Arroyo Mühr
International Agency for Research on Cancer, World Health Organization, Screening Group, Lyon, 69372, France.
Carina
C
Eklund
North-Western State Medical University named after I.I. Mechnikov, Department of Urology and Andrology, St. Petersburg, 191015, Russia.
Boris
B
Komyakov
St. Petersburg State University, Faculty of Medicine, St. Petersburg, 199034, Russia.
Andrey
A
Gorelov
International Agency for Research on Cancer, World Health Organization, Screening Group, Lyon, 69372, France.
Joakim
J
Dillner
International Agency for Research on Cancer, World Health Organization, Screening Group, Lyon, 69372, France.
Emilie
E
Hultin
D012634:Bodily Secretions / Q000821:virology*; D016022:Case-Control Studies; D019295:Computational Biology; D004267:DNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D056186:Metagenomics; D011471:Prostatic Neoplasms / Q000821:virology*; D017422:Sequence Analysis, DNA