DNA interaction, antitumor and antimicrobial activities of three-dimensional chitosan ring produced from the body segments of a diplopod.
Commercially available chitins and the chitin isolated from mushrooms, insect cuticles, shells of shrimp, crab and crayfish reported in the literature are in forms of powder, flake or granule. Three-dimensional chitins have been only known from the sponges but still three-dimensional chitosan has not been reported yet. In this study, we produced three-dimensional chitin and chitosan rings from the body segments of a diplopod species (Julus terrestris). Obtained chitin and chitosan rings were characterized (by FT-IR, SEM, TGA, XRD, dilute solution viscometry and EA) and compared with commercial chitin and chitosan. The interactions with plasmid DNA was studied at varying concentrations of chitosan (0.04, 0.4 and 4mg/mL). Antitumor activity tests were conducted (L929 and HeLa), low cytotoxicity and high antiproliferative activity was observed. Antimicrobial activities of J. terrestris chitosan were investigated on twelve microorganisms and maximum inhibition (15.6±1.154mm) was recorded for common human pathogen Staphylococcus aureus.
Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey. Electronic address: muratkaya3806@yahoo.com.
Murat
M
Kaya
Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
Bahar
B
Akyuz
Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
Esra
E
Bulut
Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
Idris
I
Sargin
Department of Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
Gamze
G
Tan
Department of Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
Demet
D
Erdonmez
Faculty of Natural Sciences, Vytautas Magnus University, LT-3000 Kaunas, Lithuania.
Mansi
M
Maheta
Faculty of Natural Sciences, Vytautas Magnus University, LT-3000 Kaunas, Lithuania.
Saulius
S
Satkauskas
Faculty of Natural Sciences, Vytautas Magnus University, LT-3000 Kaunas, Lithuania.
Saulius
S
Mickevičius
D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001181:Arthropods / Q000737:chemistry* / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D013211:Staphylococcus aureus / Q000187:drug effects
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D004247:DNA; D048271:Chitosan
Antibacterial; Biopolymer; Chitin; Cytotoxicity; HeLa cell line; Millipede