[Pacilitaxel induces human nasopharyngeal carcinoma cell line CNE2 apoptosis and growth inhibition by suppressing PI3K/AKT/p53 signaling pathway].
OBJECTIVE
To investigate the effect and mechanisms of the PTX on the human nasopharyngeal carcinoma cell line CNE2.


METHODS
Cells from CNE2 were cultured in vitro and the cells at logarithmic growth phase were processed with different concentration of PTX (0, 5, 10, 20) mol/L for 72h. MTT was used to evaluate the proliferation and flow cytometric analysis was utilized to detect membrane potential and apoptosis of CNE2 cells. The expression of PI3K, p-AKT, AKT, p53, p21, Caspase3, Cleavage-Caspase3, PARP, Cleavage-PARP, AIF, CytC, Bcl-2 and Bax in CNE2 cells were examined by Western Blot.


RESULTS
The results showed that PTX could increase the apoptosis and the expression of Caspase3, PARP, CytC, AIF and Bax and reduce the proliferation, membrane potential and the expression of PI3K, p-AKT, p53, p21, Cleavage-PARP, Cleavage-Caspase3 and Bcl-2 in CNE2 cell in a concentration-dependent manner. However, PTX had no effect on the expression of AKT.


CONCLUSIONS
PTX can promote apoptosis and growth inhibition of human nasopharyngeal cancer cell line CNE2 and the mechanism involves suppressing PI3K/AKT/p53 signaling pathway.
Tao
T
Li
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002277:Carcinoma; D045744:Cell Line, Tumor / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D008564:Membrane Potentials; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D017239:Paclitaxel