HIF-1α Silencing Inhibits the Growth of Osteosarcoma Cells by Inducing Apoptosis.
BACKGROUND
Osteosarcoma (OS) is a malignant tumor of mesenchymal origin, which is generally locally aggressive and tends to produce early systemic metastases. Therefore, an identification of a novel therapeutic target is required.


METHODS
We investigated the effect of small interfering RNA (siRNA) targeting hypoxia-inducible factor 1α (HIF-1α) on the growth of Osteosarcoma MG-63 and U2-OS cells. Two cell-lines were transfected with various concentrations of HIF-1α or control siRNA, and the effect on HIF-1α expression was analyzed by using quantitative polymerase chain reaction and western blot analysis. The effects of HIF-1α siRNA on growth inhibition and apoptosis were then evaluated by standard methods.


RESULTS
HIF-1α siRNA treatment reduced HIF-1α mRNA and protein expression in MG-63 and U2-OS cells significantly. The downregulation of HIF-1α expression upregulated caspase-3 expression subsequently inhibited the growth of MG-63 and U2-OS cells and induced apoptosis of the two cells. Our in vitro data indicate that the downregulation of HIF-1α is capable of suppressing OS cells growth, through the induction of apoptosis. HIF-1α inhibition may be a new strategy for the treatment of Osteosarcoma.
Department of Orthopedics, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei.
Fei
F
Lv
Postgraduate School, Hebei Medical University, Shijiazhuang Hebei, China.
Rui
R
Du
Department of Orthopedics, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei.
Wenqiang
W
Shang
Department of Orthopedics, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei.
Sai
S
Suo
Department of Orthopedics, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei.
Dangen
D
Yu
Department of Orthopedics, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei gukezhangjianhe@163.com.
Jianhe
J
Zhang
D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D053148:Caspase 3
HIF-1α; Osteosarcoma; RNA interference; target therapy