Cleaning and Disinfection of Bacillus cereus Biofilm.
Methodology has been evolving for the testing of disinfectants against bacterial single-species biofilms, as the difficulty of biofilm remediation continues to gain much-needed attention. Bacterial single-species biofilm contamination presents a real risk to good manufacturing practice-regulated industries. However, mixed-species biofilms and biofilms containing bacterial spores remain an even greater challenge for cleaning and disinfection. Among spore-forming microorganisms frequently encountered in pharmaceutical manufacturing areas, the spores of Bacillus cereus are often determined to be the hardest to disinfect and eradicate. One of the reasons for the low degree of susceptibility to disinfection is the ability of these spores to be encapsulated within an exopolysachharide biofilm matrix. In this series of experiments, we evaluated the disinfectant susceptibility of B. cereus biofilms relative to disassociated B. cereus spores and biofilm from a non-spore-forming species. Further, we assessed the impact that pre-cleaning has on increasing that susceptibility.
Methodology has been evolving for the testing of disinfectants against bacterial single-species biofilms, as the difficulty of biofilm remediation continues to gain much-needed attention. Bacterial single-species biofilm contamination presents a real risk to good manufacturing practice-regulated industries. However, mixed-species biofilms and biofilms containing bacterial spores remain an even greater challenge for cleaning and disinfection. Among spore-forming microorganisms frequently encountered in pharmaceutical manufacturing areas, the spores of Bacillus cereus are often determined to be the hardest to disinfect and eradicate. One of the reasons for the low degree of susceptibility to disinfection is the ability of these spores to be encapsulated within an exopolysachharide biofilm matrix. In this series of experiments, we evaluated the disinfectant susceptibility of B. cereus biofilms relative to disassociated B. cereus spores and biofilm from a non-spore-forming species. Further, we assessed the impact that pre-cleaning has on increasing that susceptibility.
STERIS Corporation, 7405 Page Avenue, St. Louis, MO 63133.
Amanda
A
Deal
STERIS Corporation, 7405 Page Avenue, St. Louis, MO 63133.
Dan
D
Klein
STERIS Corporation, 7405 Page Avenue, St. Louis, MO 63133 paul_lopolito@steris.com.
Paul
P
Lopolito
STERIS Corporation, 7405 Page Avenue, St. Louis, MO 63133.
John Spencer
JS
Schwarz
D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004202:Disinfectants / Q000008:administration & dosage; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D013171:Spores, Bacterial / Q000187:drug effects* / Q000254:growth & development
D004202:Disinfectants
ASTM; ATP; Alkaline cleaner; Bacillus cereus; Bacterial spores; Biofilm; Biofilm reactor; Cleaning; Disinfection; Pseudomonas aeruginosa; Single-tube method; Sporicide