Pectinous cell wall thickenings formation - A common defense strategy of plants to cope with Pb.
Lead, one of the most abundant and hazardous trace metals affecting living organisms, has been commonly detected in plant cell walls including some tolerant plants, mining ecotypes and hyperaccumulators. We have previously shown that in tip growing Funaria sp. protonemata cell wall is remodeled in response to lead by formation of thickenings rich in low-methylesterified pectins (pectin epitope JIM5 - JIM5-P) able to bind metal ions, which accumulate large amounts of Pb. Hence, it leads to the increase of cell wall capacity for Pb compartmentalization. Here we show that diverse plant species belonging to different phyla (Arabidopsis, hybrid aspen, star duckweed), form similar cell wall thickenings in response to Pb. These thickenings are formed in tip growing cells such as the root hairs, and in diffuse growing cells such as meristematic and root cap columella cells of root apices in hybrid aspen and Arabidopsis and in mesophyll cells in star duckweed fronds. Notably, all analyzed cell wall thickenings were abundant in JIM5-P and accumulated high amounts of Pb. In addition, the co-localization of JIM5-P and Pb commonly occurred in these cells. Hence, cell wall thickenings formed the extra compartment for Pb accumulation. In this way plant cells increased cell wall capacity for compartmentalization of this toxic metal, protecting protoplast from its toxicity. As cell wall thickenings occurred in diverse plant species and cell types differing in the type of growth we may conclude that pectinous cell wall thickenings formation is a widespread defense strategy of plants to cope with Pb. Moreover, detection of natural defense strategy, increasing plant cell walls capacity for metal accumulation, reveals a promising direction for enhancing plant efficiency in phytoremediation.
Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland. Electronic address: magdak@amu.edu.pl.
Magdalena
M
Krzesłowska
Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Irena
I
Rabęda
Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Aneta
A
Basińska
Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Michał
M
Lewandowski
Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90183 Umea, Sweden.
Ewa J
EJ
Mellerowicz
Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Anna
A
Napieralska
Laboratory of Electron and Confocal Microscopy, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Sławomir
S
Samardakiewicz
Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Adam
A
Woźny
D017360:Arabidopsis / Q000378:metabolism; D029064:Araceae / Q000378:metabolism; D001673:Biodegradation, Environmental; D019068:Bryopsida / Q000166:cytology* / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D007854:Lead / Q000378:metabolism*; D018519:Meristem / Q000378:metabolism; D010368:Pectins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D032107:Populus / Q000378:metabolism
D007854:Lead; D010368:Pectins
Diffuse growth; Heavy metal; Root; Tip growth; Tolerance