Lack of genotoxic potential of pesticides, spinosad, imidacloprid and neem oil in mice (Mus musculus).
Pesticides, spinosad, imidacloprid and neem oil are widely used both in residential and agricultural environments because of its broad spectrum insecticidal activity and effectiveness. The present study was undertaken to estimate genotoxicity of formulations of some pesticides in mice. Three pesticides of diverse group studied were spinosad (45% w/v), imidacloprid (17.8%, w/v) and neem oil. Animals were exposed 37, 4.5 and 50 mg kg⁻¹ b.wt. for spinosad, imidacloprid and neem oil, respectively, through oral gavage for 5 consecutive days. A vehicle control group and one positive control (cyclophosphamide; 20 mg kg⁻¹ b. wt.) were also selected. The results showed that cyclophosphamide produced 1.12% micronuclei in mice, as against 0.18 in vehicle control, 0.30 in spinosad, 0.28 in imidacloprid and 0.22% in neem oil, respectively. The gross percentage of chromosomal aberration in mice were 28.5% in cyclophosphamide against 6.5% in vehicle control, 8.0% in spinosad, 9.5% in imidacloprid and 7.0% in neem oil, respectively. The overall findings of the present study revealed that all the three pesticide formulations, imidacloprid, spinosad and neem oil at tested dose did not show any genotoxic effect in mice.
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D000818:Animals; D002869:Chromosome Aberrations / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005989:Glycerides / Q000633:toxicity*; D007093:Imidazoles / Q000633:toxicity*; D018942:Macrolides / Q000633:toxicity*; D051379:Mice; D015162:Micronucleus Tests; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D013729:Terpenes / Q000633:toxicity*
D004338:Drug Combinations; D005989:Glycerides; D007093:Imidazoles; D018942:Macrolides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010575:Pesticides; D013729:Terpenes; C082359:imidacloprid; C002443:neem oil; C415329:spinosad