Accelerated cloning of a potato late blight-resistance gene using RenSeq and SMRT sequencing.
Global yields of potato and tomato crops have fallen owing to potato late blight disease, which is caused by Phytophthora infestans. Although most commercial potato varieties are susceptible to blight, many wild potato relatives show variation for resistance and are therefore a potential source of Resistance to P. infestans (Rpi) genes. Resistance breeding has exploited Rpi genes from closely related tuber-bearing potato relatives, but is laborious and slow. Here we report that the wild, diploid non-tuber-bearing Solanum americanum harbors multiple Rpi genes. We combine resistance (R) gene sequence capture (RenSeq) with single-molecule real-time (SMRT) sequencing (SMRT RenSeq) to clone Rpi-amr3i. This technology should enable de novo assembly of complete nucleotide-binding, leucine-rich repeat receptor (NLR) genes, their regulatory elements and complex multi-NLR loci from uncharacterized germplasm. SMRT RenSeq can be applied to rapidly clone multiple R genes for engineering pathogen-resistant crops.
The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
Kamil
K
Witek
The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
Florian
F
Jupe
The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
Agnieszka I
AI
Witek
The Genome Analysis Centre (TGAC), Norwich Research Park, Norwich, UK.
David
D
Baker
The Genome Analysis Centre (TGAC), Norwich Research Park, Norwich, UK.
Matthew D
MD
Clark
The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
Jonathan D G
JD
Jones
D003001:Cloning, Molecular / Q000379:methods; D003199:Computer Systems; D005656:Fungal Proteins / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D010838:Phytophthora / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology* / Q000517:prevention & control; D011198:Solanum tuberosum / Q000235:genetics* / Q000382:microbiology*
D005656:Fungal Proteins