Effects of dietary conjugated linoleic acids on cellular immune response of piglets after cyclosporin A injection.
The present study investigated the effects of dietary conjugated linoleic acid (CLA) on the cellular immune response of piglets after cyclosporin A (CsA) treatment. The experimental study had a 2×2 factorial design, and the main factors consisted of diets (0% or 2% CLA) and immunosuppression treatments (CsA or saline injection). CsA injection significantly increased feed : gain (F : G) of piglets (P<0.05); however, dietary CLA significantly decreased F : G of piglets (P<0.05). Dietary CLA partly ameliorated the deterioration of the feed conversion rate caused by CsA treatment (P<0.01). CsA treatment significantly decreased the percentages of CD4+ and CD8+ T lymphocytes in the thymus (P<0.01). Dietary CLA increased the percentages of CD4+ CD8+ double-positive and CD8+ single-positive T lymphocytes in the thymus (P<0.05), and had the trend to inhibit the decrease of CD4+ T lymphocytes in the thymus after CsA injection (P=0.07). CsA treatment significantly depleted the peripheral blood CD3+, CD4+ and CD8+ T lymphocytes (P<0.01). Dietary CLA significantly increased the number of peripheral blood CD8+ T lymphocytes and interleukin-2 (IL-2) production (P<0.05), and inhibited the decreases of peripheral blood CD3+, CD4+ and CD8+ T lymphocytes counts (P<0.01) as well as IL-2 production (P<0.05) after CsA treatment. Dietary CLA partly rescued the decrease of lymphocyte proliferation after CsA injection (P<0.05). In summary, dietary CLA effectively ameliorated CsA-induced cellular immunosuppression in piglets.
1College of Animal Husbandry Engineering,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,Henan Province,P. R. China.
Y X
YX
Liu
1College of Animal Husbandry Engineering,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,Henan Province,P. R. China.
K Y
KY
Zhu
2Henan Engineering Technology Research Center of Animal Nutrition and Feed,Zhengzhou 450046,Henan Province,P. R. China.
Y L
YL
Liu
2Henan Engineering Technology Research Center of Animal Nutrition and Feed,Zhengzhou 450046,Henan Province,P. R. China.
D F
DF
Jiang
D000818:Animals; D016572:Cyclosporine / Q000008:administration & dosage*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D007111:Immunity, Cellular / Q000187:drug effects*; D007273:Injections, Intramuscular / Q000662:veterinary; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*
D044243:Linoleic Acids, Conjugated; D016572:Cyclosporine
cellular immunity; conjugated linoleic acids; cyclosporin A; piglet