Synthetic protein interactions reveal a functional map of the cell.
To understand the function of eukaryotic cells, it is critical to understand the role of protein-protein interactions and protein localization. Currently, we do not know the importance of global protein localization nor do we understand to what extent the cell is permissive for new protein associations - a key requirement for the evolution of new protein functions. To answer this question, we fused every protein in the yeast Saccharomyces cerevisiae with a partner from each of the major cellular compartments and quantitatively assessed the effects upon growth. This analysis reveals that cells have a remarkable and unanticipated tolerance for forced protein associations, even if these associations lead to a proportion of the protein moving compartments within the cell. Furthermore, the interactions that do perturb growth provide a functional map of spatial protein regulation, identifying key regulatory complexes for the normal homeostasis of eukaryotic cells.
Mitotic Control Laboratory, The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.
Lisa K
LK
Berry
Mitotic Control Laboratory, The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.
Guðjón
G
Ólafsson
Mitotic Control Laboratory, The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.
Elena
E
Ledesma-Fernández
Mitotic Control Laboratory, The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.
Peter H
http://orcid.org/0000-0003-1649-6816
PH
Thorpe
D011485:Protein Binding; D060066:Protein Interaction Maps; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D029701:Saccharomyces cerevisiae Proteins
GBP; GFP; S. cerevisiae; cell biology; localization; nanobody