Detection and Distribution of V1016Ikdr Mutation in the Voltage-Gated Sodium Channel Gene in Aedes aegypti (Diptera: Culicidae) Populations From Sergipe State, Northeast Brazil.
Aedes aegypti (L.) resistance to pyrethroids was recorded in Brazil few years after its introduction as the adulticide in the National Dengue Control Program campaigns. Altered susceptibility to pyrethroids had been reported in the state of Sergipe, northeast Brazil, through biological assays, even before its use against Ae. aegypti in the state. Metabolic and target-site resistance mechanisms were also revealed in samples from Aracaju, the capital of Sergipe. Herein, we investigated the presence and distribution of the kdr mutation V1016Ikdrin Ae. aegypti populations from different municipalities of the state. Aedes aegypti eggs were collected from seven municipalities located in areas showing different climatic types and infestation levels. Approximately 20 Ae. aegypti females from each municipality (total of 135 subjects) were individually submitted to allele-specific polymerase chain reaction (AS-PCR) for the 1016 site of the voltage-gated sodium channel (NaV). The V1016Ikdrmutation was found in subjects from all the municipalities under study with a high frequency of heterozygotes in several locations. Homozygous recessive subjects (resistant kdr genotype) were found only in one municipality. The results suggest a wide distribution of the V1016Ikdrmutation in the northeast Brazil, which indicates urgent need for monitoring the effectiveness of the pyrethroids currently used for vector control.
Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil (dolabella@ufs.br; rlacorte@ufs.br; massia_consuelo@hotmail.com; liza.ms@ig.com.br; sonajain24@yahoo.com), dolabella@ufs.br.
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Dolabella
Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil (dolabella@ufs.br; rlacorte@ufs.br; massia_consuelo@hotmail.com; liza.ms@ig.com.br; sonajain24@yahoo.com).|Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil (dolabella@ufs.br; rlacorte@ufs.br; massia_consuelo@hotmail.com; liza.ms@ig.com.br; sonajain24@yahoo.com).
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Santos
Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil (dolabella@ufs.br; rlacorte@ufs.br; massia_consuelo@hotmail.com; liza.ms@ig.com.br; sonajain24@yahoo.com).
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Silva
Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil (dolabella@ufs.br; rlacorte@ufs.br; massia_consuelo@hotmail.com; liza.ms@ig.com.br; sonajain24@yahoo.com).
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Steffler
Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil (pribolla@ibb.unesp.br).
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Ribolla
Laboratório de Química Medicinal, Departamento de Farmácia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil (socrates@ufs.br), and.
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Cavalcanti
Laboratório de Entomologia e Parasitologia Tropical, Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil (dolabella@ufs.br; rlacorte@ufs.br; massia_consuelo@hotmail.com; liza.ms@ig.com.br; sonajain24@yahoo.com).
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Jain
Laboratório de Fisiologia e Controle de Artrópodes Vetores, IOC - Fiocruz, Rio de Janeiro, Brazil (ademir.fiocruz@gmail.com).
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Martins
D000330:Aedes / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D001938:Brazil; D005260:Female; D005838:Genotype; D019476:Insect Proteins / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology*; D009154:Mutation; D016133:Polymerase Chain Reaction; D011722:Pyrethrins / Q000494:pharmacology*; D061566:Voltage-Gated Sodium Channels / Q000235:genetics*
D019476:Insect Proteins; D007306:Insecticides; D011722:Pyrethrins; D061566:Voltage-Gated Sodium Channels
Aedes aegypti; kdr mutation; pyrethroid resistance; sodium channel