Normal phase HPLC-based activity profiling of non-polar crude plant extracts - acetylcholinesterase inhibiting guttiferones from Montrouziera cauliflora as a case study.
The study describes bioactive compounds as inhibitors of acetylcholinesterase (AChE), from the stem bark extract of Montrouziera cauliflora, selected among 19 dichloromethane extracts from Clusiaceae species. Our work focused on the development of an original normal phase HPLC microfractionation strategy to rapidly assess highly active zones from this crude active non-polar plant extract. Two different microfraction collection methods were evaluated for the assessment of the AChE inhibition. Two guttiferones and a tocotrienol were directly isolated among five compounds identified off-line by NMR after upscaling the fractionation and their AChE inhibition was evaluated. The strengths and weaknesses of the two microfractionation collection methods for HPLC-AChE activity-based profiling are discussed.
a Faculty of Pharmacy , Université d'Angers , Angers , France.|b Voluntary Researcher for Institut de Chimie de Nice , Université de Nice-Sophia Antipolis/CNRS , Nice , France.
Anne
A
Landreau
c School of Pharmaceutical Sciences, EPGL , University of Geneva , University of Lausanne , Geneva , Switzerland.|d Groupe Mer, Molécules, Santé, UFR des Sciences Pharmaceutiques et Biologiques , Université de Nantes , Nantes , France.|e Institut de chimique et Biology (P2-H57) An Ninh industrielle Zone (Lai Yen) , Hanoi , Vietnam.
Samuel
S
Bertrand
c School of Pharmaceutical Sciences, EPGL , University of Geneva , University of Lausanne , Geneva , Switzerland.|d Groupe Mer, Molécules, Santé, UFR des Sciences Pharmaceutiques et Biologiques , Université de Nantes , Nantes , France.
Claudia
C
Simoes-Pires
c School of Pharmaceutical Sciences, EPGL , University of Geneva , University of Lausanne , Geneva , Switzerland.|d Groupe Mer, Molécules, Santé, UFR des Sciences Pharmaceutiques et Biologiques , Université de Nantes , Nantes , France.
Laurence
L
Marcourt
a Faculty of Pharmacy , Université d'Angers , Angers , France.|f Institut de Chimie des Substances Naturelles CNRS UPR 2301 , University Paris-Saclay , Gif-sur-Yvette , France.
Dang Bach
DB
Taï
f Institut de Chimie des Substances Naturelles CNRS UPR 2301 , University Paris-Saclay , Gif-sur-Yvette , France.
Marc
M
Litaudon
a Faculty of Pharmacy , Université d'Angers , Angers , France.
David
D
Guilet
a Faculty of Pharmacy , Université d'Angers , Angers , France.
Pascal
P
Richomme
c School of Pharmaceutical Sciences, EPGL , University of Geneva , University of Lausanne , Geneva , Switzerland.|d Groupe Mer, Molécules, Santé, UFR des Sciences Pharmaceutiques et Biologiques , Université de Nantes , Nantes , France.
Pierre-Alain
PA
Carrupt
c School of Pharmaceutical Sciences, EPGL , University of Geneva , University of Lausanne , Geneva , Switzerland.|d Groupe Mer, Molécules, Santé, UFR des Sciences Pharmaceutiques et Biologiques , Université de Nantes , Nantes , France.
Jean-Luc
JL
Wolfender
Clusiaceae; Guttiferone; HPLC microfractionation; NP HPLC; acetylcholinesterase