Fourth generation assays for HIV testing.
Laboratory diagnosis of HIV infection is essential for the prevention of infection and the identification of infected individuals who could benefit from highly active antiretroviral therapy. Since the release of the first assays for the detection of anti-HIV antibodies, the technology of immunoassays has improved.



Fourth generation assays - simultaneously detecting HIV p24 antigen and antibodies - have been developed and have been a major improvement in the detection of HIV infection, with a reduction of the diagnostic window. Studies have provided definite evidence for their clinical utility. Combination assays with separate results for anti-HIV antibodies and p24 antigen have been developed. Expert Commentary: In conclusion, fourth generation assays are an effective tool for the laboratory diagnosis of HIV infection. The ADVIA Centaur HIV Ag/Ab Combo assay is in line with most recent fourth generation assays and its clinical utility has been assessed.
a Dipartimento di Scienze Mediche Traslazionali , Università di Napoli Federico II , Napoli , Italy.
Luca
L
Vallefuoco
a Dipartimento di Scienze Mediche Traslazionali , Università di Napoli Federico II , Napoli , Italy.
Claudia
C
Mazzarella
a Dipartimento di Scienze Mediche Traslazionali , Università di Napoli Federico II , Napoli , Italy.
Giuseppe
G
Portella
D015492:AIDS Serodiagnosis / Q000379:methods*; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D015658:HIV Infections / Q000097:blood* / Q000276:immunology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
HIV-1; HIV-2; anti-HIV antibodies; antigen p24; combination immunoassays; diagnostic window; infection diagnosis