iPSC-derived mesenchymal stromal cells are less supportive than primary MSCs for co-culture of hematopoietic progenitor cells.
In vitro culture of hematopoietic stem and progenitor cells (HPCs) is supported by a suitable cellular microenvironment, such as mesenchymal stromal cells (MSCs)-but MSCs are heterogeneous and poorly defined. In this study, we analyzed whether MSCs derived from induced pluripotent stem cells (iPS-MSCs) provide a suitable cellular feeder layer too. iPS-MSCs clearly supported proliferation of HPCs, maintenance of a primitive immunophenotype (CD34(+), CD133(+), CD38(-)), and colony-forming unit (CFU) potential of CD34(+) HPCs. However, particularly long-term culture-initiating cell (LTC-IC) frequency was lower with iPS-MSCs as compared to primary MSCs. Relevant genes for cell-cell interaction were overall expressed at similar level in MSCs and iPS-MSCs, whereas VCAM1 was less expressed in the latter. In conclusion, our iPS-MSCs support in vitro culture of HPCs; however, under the current differentiation and culture conditions, they are less suitable than primary MSCs from bone marrow.
Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Pauwelsstrasse 20, 52074, Aachen, Germany.|Institute for Biomedical Engineering-Cell Biology, University Hospital of RWTH Aachen, Aachen, Germany.
Theresa
T
Vasko
Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Pauwelsstrasse 20, 52074, Aachen, Germany.|Institute for Biomedical Engineering-Cell Biology, University Hospital of RWTH Aachen, Aachen, Germany.
Joana
J
Frobel
Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Pauwelsstrasse 20, 52074, Aachen, Germany.|Institute for Biomedical Engineering-Cell Biology, University Hospital of RWTH Aachen, Aachen, Germany.
Richard
R
Lubberich
Department of Obstetrics and Gynecology, RWTH Aachen University Hospital, Aachen, Germany.
Tamme W
TW
Goecke
Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Pauwelsstrasse 20, 52074, Aachen, Germany. wwagner@ukaachen.de.|Institute for Biomedical Engineering-Cell Biology, University Hospital of RWTH Aachen, Aachen, Germany. wwagner@ukaachen.de.
Wolfgang
W
Wagner
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; C050361:CDH2 protein, human; D015820:Cadherins; D006023:Glycoproteins; C478221:LAMB1 protein, human; D007797:Laminin; C000606023:PROM1 protein, human; D010455:Peptides; D019010:Vascular Cell Adhesion Molecule-1