Differential abundances of four forms of Binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability.
The Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P < 0.05). The protein spot weighing 15.52 ± 0.53 kDa with a pI value of 5.78 ± 0.12 is highlighted by its high abundance in bulls with low semen freezability and its absence in bulls presenting high semen freezability. This is the first report showing that more than two forms of BSP1 are found in the seminal plasma of Nelore adult bulls and not all animals have a similar abundance of each BSP1 form. Different BSP1 forms may be involved in different events of fertilization and the cryopreservation process.
Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Marcos Jorge
MJ
Magalhães
Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Leonardo Franco
LF
Martins
Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Renato Lima
RL
Senra
Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Thaís Ferreira Dos
TF
Santos
Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Denise Silva
DS
Okano
Department of Plant Science, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Paulo Roberto Gomes
PR
Pereira
Department of Computer Science-ICEX, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Alessandra
A
Faria-Campos
Department of Computer Science-ICEX, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Sérgio Vale Aguiar
SV
Campos
Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
José Domingos
JD
Guimarães
Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: baracat@ufv.br.
Maria Cristina
MC
Baracat-Pereira
D000818:Animals; D002417:Cattle / Q000235:genetics / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D005615:Freezing; D008297:Male; D020033:Protein Isoforms; D012661:Semen / Q000737:chemistry* / Q000502:physiology; D012662:Semen Preservation / Q000662:veterinary*; D029607:Seminal Vesicle Secretory Proteins / Q000145:classification / Q000378:metabolism*
D020033:Protein Isoforms; D029607:Seminal Vesicle Secretory Proteins
BSP1; Computational tools; Freezability; Nelore bulls; Proteomics; Semen cryopreservation