KvLEA, a New Isolated Late Embryogenesis Abundant Protein Gene from Kosteletzkya virginica Responding to Multiabiotic Stresses.
The LEA proteins are a kind of hydrophilic proteins, playing main functions in desiccation tolerance. However, their importance as a kind of stress proteins in abiotic stress is being clarified little by little. In this study we isolated, cloned, and identified the first KvLEA gene in Kosteletzkya virginica. Bioinformatic analysis showed that the protein encoded by this gene had common properties of LEA proteins and the multiple sequences alignment and phylogenetic analysis further showed that this protein had high homology with two Arabidopsis LEA proteins. Gene expression analysis revealed that this gene had a higher expression in root and it was induced obviously by salt stress. Moreover, the transcripts of KvLEA were also induced by other abiotic stresses including drought, high temperature, chilling, and ABA treatment. Among these abiotic stresses, ABA treatment brought about the biggest changes to this gene. Collectively, our research discovered a novel LEA gene and uncovered its involvement in multiabiotic stresses in K. virginica. This research not only enriched studies on LEA gene in plant but also would accelerate more studies on K. virginica in the future.
Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Xiaoli
X
Tang
Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China; Yantai Academy of China Agricultural University, Yantai 264670, China.
Hongyan
H
Wang
The Central Laboratory, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Liye
L
Chu
Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China; Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Institute of Agro-Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Hongbo
H
Shao
D017360:Arabidopsis / Q000235:genetics; D055864:Droughts; D047108:Embryonic Development / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D019660:Malvaceae / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*
D010940:Plant Proteins; D011971:Receptors, Immunologic