A computational method for prediction of rSNPs in human genome.
Regulatory single nucleotide polymorphisms (rSNPs) in human genomes are thought to be responsible for phenotypic differences, including susceptibility to diseases and treatment outcomes, even they do not change any gene product. However, a genome-wide search for rSNPs has not been properly addressed so far. In this work, a computational method for rSNP identification is proposed. As background SNPs far outnumber rSNPs, an ensemble method is applied to handle imbalanced data, which firstly converts an unbalanced dataset into several balanced ones and then models for every balanced dataset. Two major types of features are extracted, that are sequence based features and allele-specific based features. Then random forest is applied to build the recognition model for each balanced dataset. Finally, ensemble strategies are adopted to combine the result of each model together. We have tested our method on a set of experimentally verified rSNPs, and leave-one-out cross-validation results showed that our method can achieve accuracy with sensitivity of 73.8%, specificity of 71.8% and the area under ROC curve (AUC) is 0.756. In addition, our method is threshold free and doesn't rely on data of regulatory elements, thus it will have better adaptability when facing different data scenarios. The original data and the source matlab codes involved are available at https://sourceforge.net/projects/rsnpdect/.
Ministry of Education Key Lab for Intelligent Networks and Network Security, Xi'an Jiaotong University, Xi'an 710049, PR China.
Rong
R
Li
Ministry of Education Key Lab for Intelligent Networks and Network Security, Xi'an Jiaotong University, Xi'an 710049, PR China. Electronic address: jqhan@mail.xjtu.edu.cn.
Jiuqiang
J
Han
Ministry of Education Key Lab for Intelligent Networks and Network Security, Xi'an Jiaotong University, Xi'an 710049, PR China; School of Electrical Engineering, Xi'an Jiaotong University, Xi'an 710049, PR China.
Jun
J
Liu
Ministry of Education Key Lab for Intelligent Networks and Network Security, Xi'an Jiaotong University, Xi'an 710049, PR China.
Jiguang
J
Zheng
Ministry of Education Key Lab for Intelligent Networks and Network Security, Xi'an Jiaotong University, Xi'an 710049, PR China.
Ruiling
R
Liu
D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D019992:Databases as Topic; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012372:ROC Curve
Hydroxyl radical cleavage patterns; Imbalanced data; Random forest; Regulatory SNPs