Synergistic Antibacterial Activity of Plant Peptide MBP-1 and Silver Nanoparticles Combination on Healing of Infected Wound Due to Staphylococcus aureus.
BACKGROUND
Wound infection is a common problem in hospitals and is typically caused by the antibiotic-resistant Staphylococcus aureus, which is a major pathogen for skin and soft tissue infections worldwide.


OBJECTIVE
The aim of this study was to investigate the synergistic antibacterial effect of plant peptide MBP-1 and silver nanoparticles on infected wounds caused by S. aureus.


METHODS
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of MBP-1 and silver nanoparticles both on their own and in combination form were determined against S. aureus via macrodilution and microdilution methods. The synergistic antibacterial effect of silver nanoparticles and MBP-1 was investigated on infected wounds caused by S. aureus in a mouse model.


RESULTS
The MIC and MBC of MBP-1 were found to be 0.6 and 0.7 mg/mL, respectively. MIC and MBC of silver nanoparticles were determined to be 6.25 and 12.5 mg/L, respectively. MIC and MBC of the silver nanoparticles and MBP-1 combination were found to be 3.125 mg/mL, 0.5 mg/L; and 6.25 mg/mL, 0.6 mg/L, respectively. The infected wound healed properly after the combined use of MBP-1 and silver nanoparticles.


CONCLUSIONS
The synergistic effect was found on the healing of infected wounds caused by S. aureus by using an MBP-1 and silver nanoparticles combination in a mouse model.
Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran.
Mojtaba
M
Salouti
Department of Microbiology, Faculty of Sciences, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran.
Fatemeh
F
Mirzaei
Department of Microbiology, Faculty of Sciences, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran.
Reza
R
Shapouri
Department of Microbiology, Faculty of Sciences, Zanjan Branch, Islamic Azad University, Zanjan, IR Iran.
Azam
A
Ahangari
Antibacterial Activity; Plant Peptide MBP-1; Silver Nanoparticles; Staphylococcus aureus; Wound Infection