Conjugated linoleic acid mitigates testosterone-related changes in body composition in male guinea pigs.
We hypothesize that conjugated linoleic acid (CLA) may be effective in preventing the changes in total and regional body composition and increases in interleukin (IL) 6 that occur as a result of hypogonadism. Male guinea pigs (n = 40, 70- to 72-week retired breeders) were block randomized by weight into 4 groups: (1) sham surgery (SHAM)/control (CTRL) diet, (2) SHAM/conjugated linoleic acid (CLA) diet (1%), (3) orchidectomy (ORX)/CTRL diet, and (4) ORX/CLA diet. Dual-energy x-ray absorptiometry scans were performed at baseline and week 16 to assess body composition. Serum IL-6 was analyzed using an enzyme-linked immune sorbent assay. Fatty acids (FAs) from visceral and subcutaneous adipose tissue were analyzed using gas chromatography. In ORX/CTRL guinea pigs, percent total body fat increased by 6.1%, and percent lean mass decreased by 6.7% over the 16-week treatment period, whereas no changes were observed for either parameter in ORX/CLA guinea pigs. Guinea pigs fed the CLA diet gained less percent total, upper, and lower body fat than those fed the CTRL diet regardless of surgical treatment. Regional adipose tissue FA composition was reflective of dietary FAs. Serum IL-6 concentrations were not different among groups. In this study, we observed that, in male guinea pigs, hypogonadism resulted in increased fat mass and decreased lean mass. In addition, CLA was effective in reducing gains in body fat and maintaining lean mass in both hypogonadal and intact guinea pigs.
School of Dietetics and Human Nutrition, McGill University, Montreal, QC H9X 3V9, Canada.
Susan Q
SQ
Yang
School of Dietetics and Human Nutrition, McGill University, Montreal, QC H9X 3V9, Canada.
Jason R
JR
DeGuire
School of Dietetics and Human Nutrition, McGill University, Montreal, QC H9X 3V9, Canada.
Paula
P
Lavery
School of Dietetics and Human Nutrition, McGill University, Montreal, QC H9X 3V9, Canada.
Ivy L
IL
Mak
School of Dietetics and Human Nutrition, McGill University, Montreal, QC H9X 3V9, Canada.
Hope A
HA
Weiler
Department of Exercise Science, Concordia University, Montreal, QC H4B 1R6, Canada; Nutrition, Obesity and Metabolism Lab, PERFORM Centre, Concordia University, Montreal, QC H4B 1R6, Canada. Electronic address: s.santosa@concordia.ca.
Sylvia
S
Santosa
D015502:Absorptiometry, Photon; D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D001823:Body Composition / Q000187:drug effects* / Q000502:physiology*; D005227:Fatty Acids / Q000032:analysis; D006168:Guinea Pigs; D007006:Hypogonadism; D015850:Interleukin-6 / Q000097:blood; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D008297:Male; D009919:Orchiectomy; D013739:Testosterone / Q000097:blood / Q000172:deficiency / Q000502:physiology*
D005227:Fatty Acids; D015850:Interleukin-6; D044243:Linoleic Acids, Conjugated; D013739:Testosterone
Body fat; Conjugated linoleic acid; Guinea pig; Hypogonadal; Interleukin-6; Testosterone