Comparison between intermittent and continuous spectra optia leukapheresis systems for autologous peripheral blood stem cell collection.
Terumo BCT recently introduced a new system for mononuclear cell (MNC) collection that uses a Spectra Optia apheresis machine equipped with a redesigned disposable kit and software program (version 11.2). It allows for the continuous collection of MNCs, unlike the original Spectra Optia system (version 7.2), which included a chamber for two-step cell separation. The aim of this study was to compare the two apheresis systems in regard to specific performance parameters. A retrospective data analysis of 150 patients who had undergone peripheral blood stem cell collection between March of 2014 and May of 2015 at our institution was performed. For the matched comparison, patients were divided into two groups by diagnosis and by previous forms of therapy received: a homogeneous group of patients with multiple myeloma (MM) that had received first line therapy ("MM" group, n = 88) and a heterogeneous group that included all of the other patients ("other" group, n = 62). No significant differences in CD34+ collection yields between both collection regimens were found (pMM= 0.19, pother= 0.74) in either group. Moreover, similar performance ratios (collected/predicted CD34+ cell number in %) were observed (pMM= 0.89, pother= 0.1). No relevant variations in platelet or hemoglobin loss were found between the two systems. We conclude that the new continuous Spectra Optia MNC system is equally efficient in collecting CD34+ cells and can be used without sacrificing collection efficiency levels when treating a broad variety of autologous patients. J. Clin. Apheresis 32:27-34, 2017. © 2016 Wiley Periodicals, Inc.
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Katharina
K
Lisenko
Stem Cell Laboratory, IKTZ Heidelberg GmbH, Heidelberg, Germany.
Petra
P
Pavel
Institute of Medical Biometry and Informatics, Heidelberg University, Heidelberg, Germany.
Thomas
T
Bruckner
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Joe
J
Puthenparambil
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Michael
M
Hundemer
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Anita
A
Schmitt
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Mathias
M
Witzens-Harig
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Anthony D
AD
Ho
Department of Medicine V, Heidelberg University, Heidelberg, Germany.
Patrick
P
Wuchter
D018952:Antigens, CD34 / Q000032:analysis; D064592:Autografts; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods* / Q000592:standards; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods*; D009101:Multiple Myeloma / Q000628:therapy; D000072916:Peripheral Blood Stem Cells / Q000166:cytology*; D012189:Retrospective Studies
D018952:Antigens, CD34
PBSC; autologous transplantation; collection efficiency; leukapheresis; spectra optia apheresis system