Biochemical and histological evaluations of anti-inflammatory and antioxidant p-chloro-selenosteroid actions in acute murine models of inflammation.
This study investigated the potential p-chloro-selenosteroid (PCS) anti-inflammatory effect in different animal models of acute inflammation. In order to determine a time- and a dose-curve response of action, female adult Swiss mice (25-35g) were divided in different groups and pretreated by the intragastric route (i.g.) with PCS (5-10mg/kg) and after the specific times (5, 30 and 60min) the ear inflammation was induced with croton oil (2.5%, 20μl). The ear edema, myeloperoxidase (MPO) activity and histological analyses were performed. In a second experiment, the pleurisy model was used to determine the PCS protective effect (10mg/kg, i.g., 30min before induction) in the inflammatory and oxidative alterations induced by an intrapleural injection of a 1% carrageenan solution (0.1ml) in exudate and lung samples. Dexamethasone (1mg/kg, i.g.) was used as positive control for both models. Statistical analysis was performed through a One-Way ANOVA test followed by the Newman-Keuls' test. Pretreatment of 30min with PCS, only at a dose of 10mg/kg, decreased ear edema and the MPO activity as well as the histological alterations induced by croton oil. In the pleurisy model, PCS (10mg/kg, i.g.; 30min) reduced the leukocyte counts, histological alterations, MPO and adenosine deaminase activities, oxidative damage and the non-enzymatic antioxidant defense imbalance. PCS had a similar anti-inflammatory profile to dexamethasone; however, it showed a better antioxidant effect. PCS had anti-inflammatory and antioxidant actions in two well established inflammation models in mice.
Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.
Marcel Henrique
MH
Marcondes Sari
Departamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Ana Cristina Guerra
AC
Souza
Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.
Suzan Gonçalves
SG
Rosa
Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.
Pietro Maria
PM
Chagas
Departamento de Patologia, Universidade Federal de Santa Maria, Zip Code 97105-900 RS, Brazil.
Sônia Cristina Almeida
SC
da Luz
Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil.
Oscar Endrigo Dorneles
OE
Rodrigues
Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria Zip Code 97105-900, RS, Brazil. Electronic address: criswn@ufsm.br.
Cristina Wayne
CW
Nogueira
D000208:Acute Disease; D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D007958:Leukocyte Count; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D013256:Steroids / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D009566:Nitrates; D009573:Nitrites; D013256:Steroids; C000614833:p-chloro-selenosteroid; D008315:Malondialdehyde; D002351:Carrageenan; D009195:Peroxidase; D000243:Adenosine Deaminase
Carrageenan; Croton oil; Inflammation; Mice; Organoselenium; Selenium