In Vitro Mass Propagation of Cymbopogon citratus Stapf., a Medicinal Gramineae.
Cymbopogon citratus (D.C.) Stapf. is a medicinal plant source of lemon grass oils with multiple uses in the pharmaceutical and food industry. Conventional propagation in semisolid culture medium has become a fast tool for mass propagation of lemon grass, but the production cost must be lower. A solution could be the application of in vitro propagation methods based on liquid culture advantages and automation. This chapter provides two efficient protocols for in vitro propagation via organogenesis and somatic embryogenesis of this medicinal plant. Firstly, we report the production of shoots using a temporary immersion system (TIS). Secondly, a protocol for somatic embryogenesis using semisolid culture for callus formation and multiplication, and liquid culture in a rotatory shaker and conventional bioreactors for the maintenance of embryogenic culture, is described. Well-developed plants can be achieved from both protocols. Here we provide a fast and efficient technology for mass propagation of this medicinal plant taking the advantage of liquid culture and automation.
Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas, Carretera a Camajuaní km 5,5, CP 54830, Santa Clara, Villa Clara, Cuba. elisa_quiala@yahoo.com.
Elisa
E
Quiala
Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas, Carretera a Camajuaní km 5,5, CP 54830, Santa Clara, Villa Clara, Cuba.
Raúl
R
Barbón
Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas, Carretera a Camajuaní km 5,5, CP 54830, Santa Clara, Villa Clara, Cuba.
Alina
A
Capote
Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas, Carretera a Camajuaní km 5,5, CP 54830, Santa Clara, Villa Clara, Cuba.
Naivy
N
Pérez
Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas, Carretera a Camajuaní km 5,5, CP 54830, Santa Clara, Villa Clara, Cuba.|Tropical Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 18905 SW 280th Street, Homestead, FL, 33031, USA.
Elio
E
Jiménez
D000064:Acclimatization; D019149:Bioreactors; D031743:Cymbopogon / Q000196:embryology / Q000254:growth & development* / Q000502:physiology; D018525:Germination; D063246:Organogenesis, Plant; D018520:Plant Shoots / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D010946:Plants, Medicinal / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D013242:Sterilization / Q000379:methods
Lemon grass; Medicinal plant; Organogenesis; Somatic embryogenesis; Temporary immersion system