Development of a biocompatible magnetic nanofluid by incorporating SPIONs in Amazonian oils.
Higher quality magnetic nanoparticles are needed for use as magnetic nanoprobe in medical imaging techniques and cancer therapy. Moreover, the phytochemistry benefits of some Amazonian essential oils have sparked great interest for medical treatments. In this work, a magnetic nanoprobe was developed, allying the biocompatibility and superparamagnetism of iron oxide nanoparticles (SPIONs) with benefits associated with Amazonian oils from Copaiba and Andiroba trees. SPIONs were obtained by two thermal decomposition procedures and different amounts of precursors (iron acetylacetonates). Their characterization was accomplished by Fourier transform infrared spectroscopy, thermogravimetric analysis, transmission electron microscopy (TEM), X-ray diffraction (XRD), Mössbauer spectroscopy and magnetization. The obtained nanoparticles composition and magnetic properties were not affected by the relative proportion of iron(II) and iron(III) in the precursor system. However, when changing the reducing and stabilizing agents the coating layer shows different compositions/relative weight - the more promising SPIONs have a coating mainly composed by oleylamine and an iron oxide:coating wt% ratio of 55:45. Nanoparticles size distributions were very narrow and centred in the average size of 6-7nm. Cellular assays confirmed the biocompatibility of SPIONs and their effective internalization in human colon cancer cells. Mössbauer/XRD results indicated maghemite as their main iron oxide phase, but traces of magnetite proved to be present. Magnetization saturations of 57emu/g at 5K and 42emu/g at 300K were achieved. With incorporation of SPIONs into Copaiba and Andiroba essential oils, these values show a 4-fold decrease, but the supermagnetic behaviour is preserved providing the effective formation of a nanofluid.
CFisUC, Physics Department, University of Coimbra, 3004-516 Coimbra, Portugal; CIEPQPF, Department of Chemical Engineering, University of Coimbra, 3030-790 Coimbra, Portugal.
André S
AS
Gaspar
Physics Department, Technical University of Munich, 85747 Garching, Germany.
Friedrich E
FE
Wagner
Physics Department and CICECO, University of Aveiro, 3810-193 Aveiro, Portugal.
Vítor S
VS
Amaral
UCIBIO-REQUIMTE, Department of Chemistry, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
Sofia A
SA
Costa Lima
CFisUC, Physics Department, University of Coimbra, 3004-516 Coimbra, Portugal.
Vladimir A
VA
Khomchenko
Federal University of Rondônia-UNIR, Faculty of Medicine, Laboratory of Nanomaterials and Nanobiomagnetism, CEP 76900-000, Amazonia, Brazil.
Judes G
JG
Santos
CFisUC, Physics Department, University of Coimbra, 3004-516 Coimbra, Portugal.
Benilde F O
BF
Costa
CIEPQPF, Department of Chemical Engineering, University of Coimbra, 3030-790 Coimbra, Portugal. Electronic address: luisa@eq.uc.pt.
Luísa
L
Durães
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001938:Brazil; D045744:Cell Line, Tumor; D003460:Crystallization; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D007501:Iron / Q000737:chemistry; D008280:Magnetics; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure*; D010316:Particle Size; D012987:Soil / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D015204:Spectroscopy, Mossbauer; D013818:Thermogravimetry; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D012987:Soil; D007501:Iron; D052203:Ferrosoferric Oxide
Amazonian oil; Biocompatible magnetic nanoprobe; Mössbauer spectroscopy; SPION; VSM; X-ray diffraction