Vertical transmission of Indian Ocean Lineage of chikungunya virus in Aedes aegypti and Aedes albopictus mosquitoes.
BACKGROUND
The re-emergence of chikungunya (CHIK) fever in Thailand has been caused by a novel lineage of chikungunya virus (CHIKV) termed the Indian Ocean Lineage (IOL). The Aedes albopictus mosquito is thought to be a primary vector of CHIK fever in Thailand, whereas Ae. aegypti acts as a secondary vector of the virus. The vertical transmission is believed to be a primary means to maintain CHIKV in nature and may be associated with an increased risk of outbreak. Therefore, the goal of this study was to analyze the potential of these two Thai mosquito species to transmit the virus vertically and to determine the number of successive mosquito generations for the virus transmission.


METHODS
Two-hundred-and-fifty female Ae. aegypti and Ae. albopictus mosquitoes were artificially fed a mixture of human blood and CHIKV IOL. Mosquito larvae and adults were sampled and screened for CHIKV by one-step qRT-PCR. LLC-MK2 cell line was used to isolate CHIKV in the mosquitoes each generation. The virus isolate was identified by immunocytochemical staining and was confirmed by sequencing. Both mosquito species fed on human blood without CHIKV and uninfected LLC-MK2 cells were used as controls.


RESULTS
Aedes aegypti and Ae. albopictus mosquitoes were able to transmit CHIKV vertically to F5 and F6 progenies, respectively. The virus isolated from the two mosquito species caused cytopathic effect in LLC-MK2 cells by 2 days post-infection and immunocytochemical staining showed the reaction between CHIKV IOL antigen and specific monoclonal antibody in the infected cells. DNA sequence confirmed the virus transmitted vertically as CHIKV IOL with E1-A226V mutation. No CHIKV infection was observed in both mosquito species and LLC-MK2 cells from control groups.


CONCLUSIONS
The study demonstrated that Ae. aegypti and Ae. albopictus mosquitoes from Thailand are capable of transmitting CHIKV IOL vertically in the laboratory. Our results showed that Ae. albopictus is more susceptible and has a greater ability to transmit the virus vertically than Ae. aegypti. This knowledge would be useful for risk assessments of the maintenance of CHIKV in nature, which is crucial for disease surveillance, vector control and the prevention of potential CHIKV epidemics.
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Jakkrawarn
J
Chompoosri
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Usavadee
U
Thavara
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Apiwat
A
Tawatsin
Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Rungfar
R
Boonserm
Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Atchara
A
Phumee
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Somchai
S
Sangkitporn
Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. padet.s@chula.ac.th.|Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand. padet.s@chula.ac.th.
Padet
P
Siriyasatien
D000330:Aedes / Q000821:virology*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002646:Chikungunya virus / Q000502:physiology*; D018445:Infectious Disease Transmission, Vertical; D007303:Insect Vectors / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins
Aedes aegypti; Aedes albopictus; Chikungunya virus; Indian Ocean Lineage; Vertical transmission