Photoreceptor IRBP prevents light induced injury.
Interphotoreceptor retinoid-binding protein (IRBP) is a classic inducer of experimental autoimmune uveoretinitis (EAU). Although IRBP causes neuronal loss in susceptible animals, resistant animals such as Sprague-Dawley (SPD) rats can benefit from the evoked protective autoimmune responses. The aim of the present study was to analyze the neuroprotective effects of IRBP against light-induced photoreceptor degeneration. We immunized 75 male SPD rats with IRBP and the rats were then exposed to blue light for 24 hours (IRBP group). Seventy five rats were included in the control group. We found that the number of apoptotic cells in the outer nuclear layer (ONL) peaked on 1 day after light exposure, and the ONL thickness decreased significantly on day 3. OX42-positive cells appeared in the ONL immediately after light exposure, and their number peaked on day 3, and changed from resting ramified cells to activated amoeboid cells. Compared with the control group (n=75), the IRBP group showed less apoptotic cells, a thicker ONL, and reduced expression of tumor necrosis factor-alpha. These outcomes indicate the IRPB might protect retinal photoreceptors against light-induced injury.
Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.
Zhongcui
Z
Sun
Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.
Meng
M
Zhang
Wei
W
Liu
Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China.
Jie
J
Tian
Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China, drxugezhi@yeah.net.
Gezhi
G
Xu
D000818:Animals; D017209:Apoptosis / Q000276:immunology / Q000528:radiation effects; D001327:Autoimmune Diseases / Q000209:etiology / Q000517:prevention & control; D004195:Disease Models, Animal; D008027:Light / Q000009:adverse effects*; D008297:Male; D017628:Microglia / Q000276:immunology / Q000473:pathology / Q000528:radiation effects; D018696:Neuroprotective Agents / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*; D020419:Photoreceptor Cells, Vertebrate / Q000276:immunology* / Q000473:pathology / Q000528:radiation effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012173:Retinitis / Q000209:etiology / Q000517:prevention & control; D012177:Retinol-Binding Proteins / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014605:Uveitis / Q000209:etiology / Q000517:prevention & control; D014611:Vaccination
D018696:Neuroprotective Agents; D010446:Peptide Fragments; D012177:Retinol-Binding Proteins; D014409:Tumor Necrosis Factor-alpha; C084432:immunodominant R16 protein, rat