Investigation of bias in meta-analyses due to selective inclusion of trial effect estimates: empirical study.
OBJECTIVE
To explore whether systematic reviewers selectively include trial effect estimates in meta-analyses when multiple are available, and what impact this may have on meta-analytic effects.


METHODS
Cross-sectional study.


METHODS
We randomly selected systematic reviews of interventions from 2 clinical specialties published between January 2010 and 2012. The first presented meta-analysis of a continuous outcome in each review was selected (index meta-analysis), and all trial effect estimates that were eligible for inclusion in the meta-analysis (eg, from multiple scales or time points) were extracted from trial reports.


METHODS
We calculated a statistic (the Potential Bias Index (PBI)) to quantify and test for evidence of selective inclusion. The PBI ranges from 0 to 1; values above or below 0.5 are suggestive of selective inclusion of effect estimates more or less favourable to the intervention, respectively. The impact of any potential selective inclusion was investigated by comparing the index meta-analytic standardised mean difference (SMD) to the median of a randomly constructed distribution of meta-analytic SMDs (representing the meta-analytic SMD expected when there is no selective inclusion).


RESULTS
31 reviews (250 trials) were included. The estimated PBI was 0.57 (95% CI 0.50 to 0.63), suggesting that trial effect estimates that were more favourable to the intervention were included in meta-analyses slightly more often than expected under a process consistent with random selection; however, the 95% CI included the null hypothesis of no selective inclusion. Any potential selective inclusion did not have an important impact on the meta-analytic effects.


CONCLUSIONS
There was no clear evidence that selective inclusion of trial effect estimates occurred in this sample of meta-analyses. Further research on selective inclusion in other clinical specialties is needed. To enable readers to assess the risk of selective inclusion bias, we recommend that systematic reviewers report the methods used to select effect estimates to include in meta-analyses.
Australasian Cochrane Centre, School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia School of Social and Community Medicine, University of Bristol, Bristol, UK.
Matthew J
MJ
Page
Department of Epidemiology and Preventive Medicine, School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia.
Andrew
A
Forbes
National Trauma Research Institute, Central Clinical School, Monash University, Melbourne, Victoria, Australia.
Marisa
M
Chau
Australasian Cochrane Centre, School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia.
Sally E
SE
Green
Australasian Cochrane Centre, School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia.
Joanne E
JE
McKenzie
D003430:Cross-Sectional Studies; D006801:Humans; D015201:Meta-Analysis as Topic; D012107:Research Design; D015983:Selection Bias
Bias; Randomised trials; STATISTICS & RESEARCH METHODS; Systematic reviews