[Cloning and expression analysis of 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase gene in Tripterygium wilfordii].
To clone the 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TwMCT) full length cDNA from Tripterygium wilfordii, the specific primers were designed according to the transcriptome data and the LCPCR were carried out. After a series of bioinformatics analysis on the TwMCT, the MeJA induced expression content were investigated by real-time fluorescence quantification polymerase chain reaction (RT-qPCR). The result showed that the full of TwMCTcDNA was 1 318 bp nucleotides encoding 311 amino acids. The molecular weight of the deduced TwMCT protein was about 34.14 kDa and the theoretical isoelectric point was 8.65. Result of the RT-qPCR analysis indicated that the content of TwMCT mRNA expression in T. wilfordii suspension cell was rising after treating with MeJA and reached the maximum in 24 h. Cloning and analyzing TwMCT gene from T. wilfordii provided gene element for studying the function and expression regulation of secondary metabolites.
Yu-ru
YR
Tong
Ping
P
Su
Meng
M
Zhang
Yu-jun
YJ
Zhao
Xiu-juan
XJ
Wang
Wei
W
Gao
Lu-qi
LQ
Huang
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013403:Sugar Phosphates / Q000378:metabolism; D029757:Tripterygium / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
C114232:2-C-methylerythritol 4-phosphate; D010940:Plant Proteins; D013403:Sugar Phosphates; D009713:Nucleotidyltransferases; D004896:Erythritol