MicroRNA-93 inhibits ischemia-reperfusion induced cardiomyocyte apoptosis by targeting PTEN.
MicroRNAs have been implicated in some biological and pathological processes, including the myocardial ischemia/reperfusion (I/R) injury. Recent findings demonstrated that miR-93 might provide a potential cardioprotective effect on ischemic heart disease. This study was to investigate the role of miR-93 in I/R-induced cardiomyocyte injury and the potential mechanism. In this study, we found that hypoxia/reoxygenation (H/R) dramatically increased LDH release, MDA contents, ROS generation, and endoplasmic reticulum stress (ERS)-mediated cardiomyocyte apoptosis, which were attenuated by co-transfection with miR-93 mimic. Phosphatase and tensin homolog (PTEN) was identified as the target gene of miR-93. Furthermore, miR-93 mimic significantly increased p-Akt levels under H/R, which was partially released by LY294002. In addtion, Ad-miR-93 also attenuated myocardial I/R injury in vivo, manifested by reduced LDH and CK levels, infarct area and cell apoptosis. Taken together, our findings indicates that miR-93 could protect against I/R-induced cardiomyocyte apoptosis by inhibiting PI3K/AKT/PTEN signaling.
Department of Cardiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.
Zun-Ping
ZP
Ke
Department of Cardiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.
Peng
P
Xu
Department of Emergency, The Affiliated Huai'an Hospital of Xuzhou Medical College and The Second People's Hospital of Huai'an, Huai'an, China.
Yan
Y
Shi
Department of Pharmacy, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.
Ai-Mei
AM
Gao
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
C543350:MIRN93 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase
AKT; PTEN; Pathology Section; apoptosis; ischemia/reperfusion; microRNA-93