Microtubule nucleation and organization in dendrites.
Dendrite branching is an essential process for building complex nervous systems. It determines the number, distribution and integration of inputs into a neuron, and is regulated to create the diverse dendrite arbor branching patterns characteristic of different neuron types. The microtubule cytoskeleton is critical to provide structure and exert force during dendrite branching. It also supports the functional requirements of dendrites, reflected by differential microtubule architectural organization between neuron types, illustrated here for sensory neurons. Both anterograde and retrograde microtubule polymerization occur within growing dendrites, and recent studies indicate that branching is enhanced by anterograde microtubule polymerization events in nascent branches. The polarities of microtubule polymerization events are regulated by the position and orientation of microtubule nucleation events in the dendrite arbor. Golgi outposts are a primary microtubule nucleation center in dendrites and share common nucleation machinery with the centrosome. In addition, pre-existing dendrite microtubules may act as nucleation sites. We discuss how balancing the activities of distinct nucleation machineries within the growing dendrite can alter microtubule polymerization polarity and dendrite branching, and how regulating this balance can generate neuron type-specific morphologies.
a Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute , Wako , Saitama , Japan.
Caroline
C
Delandre
a Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute , Wako , Saitama , Japan.
Reiko
R
Amikura
a Laboratory for Genetic Control of Neuronal Architecture, RIKEN Brain Science Institute , Wako , Saitama , Japan.
Adrian W
AW
Moore
D000818:Animals; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D004331:Drosophila melanogaster / Q000378:metabolism* / Q000648:ultrastructure; D006056:Golgi Apparatus; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D009024:Morphogenesis
Augmin; Golgi outpost; dendrite; microtubule nucleation; microtubule polarity; neuron morphology; pericentriolar material