Water stress induces changes in polyphenol profile and antioxidant capacity in poplar plants (Populus spp.).
This paper is aimed to characterize young poplar plants under the influence of water stress provoked by polyethileneglycol 6000 (PEG 6000). Three polar genotypes (M1, B229, and PE19/66) were grown in hydroponics and subjected to 100 and 200 mOsm PEG 6000 during six days. Polyphenol characterization, two enzymatic markers and antioxidant capacity in leaves and roots were investigated in stressed plants. Total phenol content, ferric reducing antioxidant capacity (FRAP) and DPPH antiradical power (DPPH ARP) were determined for estimating total antioxidant capacity. Polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were determined as enzymatic markers. Polyphenol characterization of poplar samples was performed by HPLC-PDA analysis. All results were subjected to correlation analysis and principal component analysis (PCA). Inspite of the decrease of total phenol content in investigated genotypes, as well as total antioxidant capacity, some of polyphenols were affected by stress like flavonoids chrysin, myricetine, kaempferol and isoferulic acid in roots of B229 genotype (Populus deltoides). Genotype B229 also showed the increase of antioxidant capacity and PAL activity in root and leaves under stress what could be the indicator of the adaptability of poplar plants to water stress. Significant positive correlations were obtained between PAL, antioxidant capacity as well as phenolic acids among themselves. Chemometric evaluation showed close interdependence between flavonoids, FRAP, DPPH antiradical power and both investigated enzymes of polyphenol metabolism, PAL and PPO.
Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia. Electronic address: popovicb@polj.uns.ac.rs.
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Popović
Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
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Štajner
Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
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Ždero-Pavlović
Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
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Tumbas-Šaponjac
Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
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Čanadanović-Brunet
Institute of Lowland Forestry and Environment, Antona Čehova 13, 21000 Novi Sad, Serbia.
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Orlović
D000975:Antioxidants / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000737:chemistry; D003681:Dehydration; D036681:Fluorescence Recovery After Photobleaching; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D059808:Polyphenols / Q000378:metabolism*; D032107:Populus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D025341:Principal Component Analysis
D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D011092:Polyethylene Glycols; C004931:1,1-diphenyl-2-picrylhydrazyl; D010650:Phenylalanine Ammonia-Lyase
Antioxidant; PCA; Polyphenol; Poplar; Water stress