Ultraviolet spectroscopy combined with ultra-fast liquid chromatography and multivariate statistical analysis for quality assessment of wild Wolfiporia extensa from different geographical origins.
A quality assessment system comprised of a tandem technique of ultraviolet (UV) spectroscopy and ultra-fast liquid chromatography (UFLC) aided by multivariate analysis was presented for the determination of geographic origin of Wolfiporia extensa collected from five regions in Yunnan Province of China. Characteristic UV spectroscopic fingerprints of samples were determined based on its methanol extract. UFLC was applied for the determination of pachymic acid (a biomarker) presented in individual test samples. The spectrum data matrix and the content of pachymic acid were integrated and analyzed by partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). The results showed that chemical properties of samples were clearly dominated by the epidermis and inner part as well as geographical origins. The relationships among samples obtained from these five regions have been also presented. Moreover, an interesting finding implied that geographical origins had much greater influence on the chemical properties of epidermis compared with that of the inner part. This study demonstrated that a rapid tool for accurate discrimination of W. extensa by UV spectroscopy and UFLC could be available for quality control of complicated medicinal mushrooms.
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, PR China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, PR China; College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, PR China.
Yan
Y
Li
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, PR China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, PR China.
Ji
J
Zhang
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, PR China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, PR China.
Hang
H
Jin
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China.
Honggao
H
Liu
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, PR China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, PR China. Electronic address: boletus@126.com.
Yuanzhong
Y
Wang
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014315:Triterpenes / Q000032:analysis; D000070660:Wolfiporia / Q000737:chemistry*
D014315:Triterpenes; C102487:pachymic acid
Geographical origins; Multivariate analysis; Quality assessment; UFLC; UV spectroscopy; Wolfiporia extensa