A triphenylcyclopropenylium mass tag: synthesis and application to ultrasensitive LC/MS analysis of amines.
Thiol adducts of triphenylcyclopropenylium undergo efficient heterolytic dissociation under conditions of both electrospray (ESI) and laser desorption ionization (LDI) mass spectrometry giving rise to a prominent signal of an aromatic C3Ph3(+) cation. A functionalized mass tagging reagent, an activated ester carrying an S-linked C3Ph3 unit, has been developed and used for the derivatization of amines and their subsequent HPLC/ESI-MS detection in low attomolar amounts.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. korshun@ibch.ru.
Artyom P
AP
Topolyan
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. korshun@ibch.ru.
Dina A
DA
Strizhevskaya
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. korshun@ibch.ru.
Maria A
MA
Belyaeva
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. korshun@ibch.ru.
Vladimir A
VA
Brylev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. korshun@ibch.ru and Lumiprobe Corp., 1835 E. Hallandale Beach Blvd. #221, Hallandale Beach, FL 33009, USA. alex.ustinov@lumiprobe.com.
Alexey V
AV
Ustinov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. korshun@ibch.ru.
Andrey A
AA
Formanovsky
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia. korshun@ibch.ru and Gause Institute of New Antibiotics, Bolshaya Pirogovskaya 11, 119021 Moscow, Russia.
Vladimir A
VA
Korshun