Tuning the inflammatory response to silver nanoparticles via quercetin in Caco-2 (co-)cultures as model of the human intestinal mucosa.
Interaction of nanoparticles with food matrix components may cause unpredictable health complications. Using an improved Caco-2 cell-based in vitro (co-)culture model the potential of quercetin as one of the major food flavonoids to alter the effect of silver nanoparticles (Ag-NPs) <20 nm in the human intestinal mucosa at real life concentrations was investigated. Ag-NPs (15-90 μg/ml) decreased cell viability and reduced thiol groups, induced oxidative/nitrosative stress and lipid peroxidation and led to activity changes of various antioxidant enzymes after 3h exposure. The contribution of Ag(+) ions within the concentrations released from nanoparticles was shown to be less important, compared to Ag-NPs. While leading to inflammatory response in the intestines, Ag-NPs, paradoxically, also showed a potential anti-infammatory effect manifested in down-regulated IL-8 levels. Quercetin, co-administered with Ag-NPs, led to a reduction of cytotoxicity, oxidative stress, and recovered metabolic activity of Caco-2 cells, suggesting the protective effects of this flavonoid against the harmful effect of Ag-NPs. Quercetin not only alleviated the effect of Ag-NPs on the gastrointestinal cells, but also demonstrated a potential to serve as a tool for reversible modulation of intestinal permeability.
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences & Université catholique de Louvain (UCL), B1348 Louvain-la-Neuve, Belgium; Interdisciplinary Nanoscience Center (iNano), Aarhus University, 8000 Aarhus C, Denmark.
Alina
A
Martirosyan
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences & Université catholique de Louvain (UCL), B1348 Louvain-la-Neuve, Belgium; School of Biosciences, University of Birmingham, United Kingdom.
Konstantinos
K
Grintzalis
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences & Université catholique de Louvain (UCL), B1348 Louvain-la-Neuve, Belgium.
Madeleine
M
Polet
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences & Université catholique de Louvain (UCL), B1348 Louvain-la-Neuve, Belgium.
Laurie
L
Laloux
Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences & Université catholique de Louvain (UCL), B1348 Louvain-la-Neuve, Belgium. Electronic address: yjs@uclouvain.be.
Yves-Jacques
YJ
Schneider
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D053768:Metal Nanoparticles; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D018570:Risk Assessment; D018030:Silver Compounds / Q000633:toxicity*
D000893:Anti-Inflammatory Agents; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D018030:Silver Compounds; D011794:Quercetin
Gastrointestinal tract; Inflammatory response; Oxidative and nitrosative stress; Quercetin; Silver nanoparticles