Comparison of Cellular Alterations in Fat Cells Harvested With Laser-Assisted Liposuction and Suction-Assisted Liposuction.
OBJECTIVE
The aim of the present study was to evaluate the viability and proliferative capacity of adipose-derived stem cells obtained by laser-assisted liposuction (LAL).


METHODS
Fat tissue was obtained from 7 male patients treated surgically for gynecomastia. On one side, harvesting was made before LAL, while it was implemented after LAL on the contralateral side. Viability, cell surface antigens, pluripotency, and apoptosis were assessed and compared in these samples.


RESULTS
Cells harvested before and after LAL did not exhibit any significant difference in terms of surface cell markers. Number of viable stem cells was lower initially after exposure to laser, while this difference was reversed at the end of 72 hours. Genetic indicators of cellular differentiation were similar in both groups. Apoptosis indicators were increased remarkably after laser exposure in the first 24 hours, but this increase was absent 72 hours after LAL procedure.


CONCLUSIONS
The authors' results have promising clinical relevance since mesenchymal stem cells harvested during LAL have maintained appropriate cellular features to be used for autologous fat transfer and fat grafting.
*Department of Plastic Reconstructive and Aesthetic Surgery, Bezmialem Vakif University†Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey.
Kemalettin
K
Yildiz
Pakize Neslihan
PN
Taşli
Fikrettin
F
Şahin
Ethem
E
Güneren
D017667:Adipocytes / Q000166:cytology*; D000328:Adult; D006801:Humans; D053685:Laser Therapy / Q000379:methods*; D015187:Lipectomy / Q000379:methods*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D013396:Suction / Q000379:methods*; D020858:Tissue and Organ Harvesting / Q000379:methods*; D055815:Young Adult