Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia.
Salmonellosis is one of the major food-borne diseases in many countries. This study was carried out to determine the occurrence of Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in raw chicken meat from wet markets and hypermarkets in Selangor, as well as to determine the antibiotic susceptibility profile of S. Enteritidis and S. Typhimurium. The most probable number (MPN) in combination with multiplex polymerase chain reaction (mPCR) method was used to quantify the Salmonella spp., S. Enteritidis, and S. Typhimurium in the samples. The occurrence of Salmonella spp., S. Enteritidis, and S. Typhimurium in 120 chicken meat samples were 20.80%, 6.70%, and 2.50%, respectively with estimated quantity varying from <3 to 15 MPN/g. The antibiogram testing revealed differential multi-drug resistance among S. Enteritidis and S. Typhimurium isolates. All the isolates were resistance to erythromycin, penicillin, and vancomycin whereas sensitivity was recorded for Amoxicillin/Clavulanic acid, Gentamicin, Tetracycline, and Trimethoprim. Our findings demonstrated that the retail chicken meat could be a source of multiple antimicrobial-resistance Salmonella and may constitute a public health concern in Malaysia.
Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia upmtty@yahoo.com.
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Thung
Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
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Mahyudin
Novel Antibiotic Laboratory, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, 50300 UKM Kuala Lumpur, Selangor Darul Ehsan, Malaysia.
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Basri
Department of Science and Technology Studies, Faculty of Science Building, Universiti of Malaya, 50603 UM Kuala Lumpur, Selangor Darul Ehsan, Malaysia.
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Wan Mohamed Radzi
Center for Southeast Asian Studies, Kyoto University, Kyoto 606-8501, Japan.
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Nakaguchi
Center for Southeast Asian Studies, Kyoto University, Kyoto 606-8501, Japan.
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Nishibuchi
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Radu
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D008296:Malaysia / Q000453:epidemiology; D008460:Meat / Q000382:microbiology*; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D015995:Prevalence; D012481:Salmonella Infections, Animal / Q000453:epidemiology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000187:drug effects*; D012486:Salmonella typhimurium / Q000187:drug effects*
Salmonella; antimicrobial susceptibility; chicken meat; most probable number PCR; prevalence