Lutibacter profundi sp. nov., isolated from a deep-sea hydrothermal system on the Arctic Mid-Ocean Ridge and emended description of the genus Lutibacter.
A bacterial strain designated LP1Twas isolated from a microbial mat growing on the surface of a black smoker chimney at the Loki's Castle hydrothermal system, which is located on the Arctic Mid-Ocean Ridge. Phylogenetic analyses based on 16S rRNA gene sequences positioned strain LP1Twithin the family Flavobacteriaceae with Lutibacterholmesii as the closest relative (97.5 % 16S rRNA gene sequence similarity). Strain LP1Twas rod-shaped, Gram-reaction-negative and non-motile. It grew in a modified artificial seawater medium supplemented with tryptone and vitamins at pH 5.5-7.5 (optimum pH 6.0-6.5), within a temperature range of 13-34 °C (optimum 23 °C), and under microaerobic conditions. The most abundant fatty acids (>10 %) were iso-C15 : 0 (25.2 %) and iso-C15 : 0 3-OH (14.5 %). The genome of strain LP1Thas a DNA G+C content of 29.8 mol%. Based on the results of the polyphasic characterization presented here, strain LP1Tis considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter profundi sp. nov. is proposed. The type strain is LP1T(=DSM 100437T=JCM 30585T). An emended description of the genus Lutibacter is also provided to fit the description of strain LP1T.
Department of Biology, Center for Geobiology, University of Bergen, P.O. Box 7800, 5020 Bergen, Norway.
Sven
S
Le Moine Bauer
Department of Biology, Center for Geobiology, University of Bergen, P.O. Box 7800, 5020 Bergen, Norway.
Irene
I
Roalkvam
Department of Biology, Center for Geobiology, University of Bergen, P.O. Box 7800, 5020 Bergen, Norway.
Ida Helene
IH
Steen
Department of Biology, Center for Geobiology, University of Bergen, P.O. Box 7800, 5020 Bergen, Norway.
Håkon
H
Dahle
D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000235:genetics; D060086:Hydrothermal Vents / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
Bacteroidetes; Northern Atlantic; hydrothermal vent field; new taxa