Exposure to environmentally persistent free radicals during gestation lowers energy expenditure and impairs skeletal muscle mitochondrial function in adult mice.
We have investigated the effects of in utero exposure to environmentally persistent free radicals (EPFRs) on growth, metabolism, energy utilization, and skeletal muscle mitochondria in a mouse model of diet-induced obesity. Pregnant mice were treated with laboratory-generated, combustion-derived particular matter (MCP230). The adult offspring were placed on a high-fat diet for 12 wk, after which we observed a 9.8% increase in their body weight. The increase in body size observed in the MCP230-exposed mice was not associated with increases in food intake but was associated with a reduction in physical activity and lower energy expenditure. The reduced energy expenditure in mice indirectly exposed to MCP230 was associated with reductions in skeletal muscle mitochondrial DNA copy number, lower mRNA levels of electron transport genes, and reduced citrate synthase activity. Upregulation of key genes involved in ameliorating oxidative stress was also observed in the muscle of MCP230-exposed mice. These findings suggest that gestational exposure to MCP230 leads to a reduction in energy expenditure at least in part through alterations to mitochondrial metabolism in the skeletal muscle.
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Erin J
EJ
Stephenson
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Alyse
A
Ragauskas
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Sridhar
S
Jaligama
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
JeAnna R
JR
Redd
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Jyothi
J
Parvathareddy
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Matthew J
MJ
Peloquin
Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Jordy
J
Saravia
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Joan C
JC
Han
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee.
Stephania A
SA
Cormier
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee; and Children's Foundation Research Institute, Le Bonheur Children's Hospital, Memphis, Tennessee davebrid@umich.edu.
Dave
0000-0002-5334-972X
D
Bridges
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D004781:Environmental Exposure / Q000009:adverse effects; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D005609:Free Radicals / Q000633:toxicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D028361:Mitochondrial Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D052638:Particulate Matter / Q000633:toxicity*; D011247:Pregnancy / Q000187:drug effects; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology
D004785:Environmental Pollutants; D005609:Free Radicals; D052638:Particulate Matter
environmentally persistent free radicals; in utero exposure; mitochondria; oxidative stress; skeletal muscle