Genome-wide analysis of gene expression during adipogenesis in human adipose-derived stromal cells reveals novel patterns of gene expression during adipocyte differentiation.
We have undertaken an in-depth transcriptome analysis of adipogenesis in human adipose-derived stromal cells (ASCs) induced to differentiate into adipocytes in vitro. Gene expression was assessed on days 1, 7, 14 and 21 post-induction and genes differentially expressed numbered 128, 218, 253 and 240 respectively. Up-regulated genes were associated with blood vessel development, leukocyte migration, as well as tumor growth, invasion and metastasis. They also shared common pathways with certain obesity-related pathophysiological conditions. Down-regulated genes were enriched for immune response processes. KLF15, LMO3, FOXO1 and ZBTB16 transcription factors were up-regulated throughout the differentiation process. CEBPA, PPARG, ZNF117, MLXIPL, MMP3 and RORB were up-regulated only on days 14 and 21, which coincide with the maturation of adipocytes and could possibly serve as candidates for controlling fat accumulation and the size of mature adipocytes. In summary, we have identified genes that were up-regulated only on days 1 and 7 or days 14 and 21 that could serve as potential early and late-stage differentiation markers.
Department of Immunology, Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, SAMRC Extramural Unit for Stem Cell Research and Therapy, University of Pretoria, South Africa. Electronic address: ambelema@gmail.com.
Melvin Anyasi
MA
Ambele
Department of Immunology, Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, SAMRC Extramural Unit for Stem Cell Research and Therapy, University of Pretoria, South Africa. Electronic address: carla.d.lu@gmail.com.
Carla
C
Dessels
Department of Immunology, Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, SAMRC Extramural Unit for Stem Cell Research and Therapy, University of Pretoria, South Africa. Electronic address: Chrisna.Durandt@up.ac.za.
Chrisna
C
Durandt
Department of Immunology, Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, SAMRC Extramural Unit for Stem Cell Research and Therapy, University of Pretoria, South Africa. Electronic address: Michael.Pepper@up.ac.za.
Michael Sean
MS
Pepper
D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D002478:Cells, Cultured; D015894:Genome, Human; D006801:Humans; D008856:Microscopy, Fluorescence; D025341:Principal Component Analysis; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
D014157:Transcription Factors; D012313:RNA
Adipocyte differentiation; Adipogenesis; Gene expression; Human adipose-derived stromal cells; Microarray; Obesity