Two Arginine Residues of Streptococcus gordonii Sialic Acid-Binding Adhesin Hsa Are Essential for Interaction to Host Cell Receptors.
Hsa is a large, serine-rich protein of Streptococcus gordonii DL1 that mediates binding to α2-3-linked sialic acid termini of glycoproteins, including platelet glycoprotein Ibα, and erythrocyte membrane protein glycophorin A, and band 3. The binding of Hsa to platelet glycoprotein Ibα contributes to the pathogenesis of infective endocarditis. This interaction appears to be mediated by a second non-repetitive region (NR2) of Hsa. However, the molecular details of the interaction between the Hsa NR2 region and these glycoproteins are not well understood. In the present study, we identified the amino acid residues of the Hsa NR2 region that are involved in sialic acid recognition. To identify the sialic acid-binding site of Hsa NR2 region, we prepared various mutants of Hsa NR2 fused with glutathione transferase. Fusion proteins harboring Arg340 to Asn (R340N) or Arg365 to Asn (R365N) substitutions in the NR2 domain exhibited significantly reduced binding to human erythrocytes and platelets. A sugar-binding assay showed that these mutant proteins abolished binding to α2-3-linked sialic acid. Furthermore, we established S. gordonii DL1 derivatives that encoded the corresponding Hsa mutant protein. In whole-cell assays, these mutant strains showed significant reductions in hemagglutination, in platelet aggregation, and in adhesion to human leukocytes. These results indicate that the Arg340 and Arg365 residues of Hsa play an important role in the binding of Hsa to α2-3-linked sialic acid-containing glycoproteins.
Department of Microbiology, Nippon Dental University School of Life Dentistry at Tokyo, Fujimi, Chiyoda-ku, Tokyo, Japan.
Yumiko
Y
Urano-Tashiro
Department of Microbiology, Nippon Dental University School of Life Dentistry at Tokyo, Fujimi, Chiyoda-ku, Tokyo, Japan.
Yukihiro
Y
Takahashi
Department of Pediatric Dentistry2, Nippon Dental University School of Life Dentistry at Tokyo, Fujimi, Chiyoda-ku, Tokyo, Japan.
Riyo
R
Oguchi
Department of Microbiology, Nippon Dental University School of Life Dentistry at Tokyo, Fujimi, Chiyoda-ku, Tokyo, Japan.
Kiyoshi
K
Konishi
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion; D004912:Erythrocytes / Q000378:metabolism; D006389:Hemagglutinins, Viral; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D054773:Streptococcus gordonii / Q000378:metabolism*
D018829:Adhesins, Bacterial; D002352:Carrier Proteins; D006389:Hemagglutinins, Viral; D011956:Receptors, Cell Surface; C450840:hsa protein, Streptococcus gordonii; D001120:Arginine; D019158:N-Acetylneuraminic Acid