Solanum pennellii backcross inbred lines (BILs) link small genomic bins with tomato traits.
We present a resource for fine mapping of traits derived from the wild tomato species Solanum pennellii (LA0716). The population of backcross inbred lines (BILs) is composed of 446 lines derived after a few generations of backcrosses of the wild species with cultivated tomato (cultivar M82; LA3475), followed by more than seven generations of self-pollination. The BILs were genotyped using the 10K SOL-CAP single nucleotide polymorphism (SNP) -Chip, and 3700 polymorphic markers were used to map recombination break points relative to the physical map of Solanum lycopersicum. The BILs carry, on average, 2.7 introgressions per line, with a mean introgression length of 11.7 Mbp. Whereas the classic 76 introgression lines (ILs) partitioned the genome into 106 mapping bins, the BILs generated 633 bins, thereby enhancing the mapping resolution of traits derived from the wild species. We demonstrate the power of the BILs for rapid fine mapping of simple and complex traits derived from the wild tomato species.
Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, 7610001, Israel.
Itai
I
Ofner
Department of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot, 7610001, Israel.|Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38010, TN, Italy.
Justin
J
Lashbrooke
Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, 7610001, Israel.
Tzili
T
Pleban
Department of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot, 7610001, Israel.
Asaph
A
Aharoni
Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, 7610001, Israel.
Dani
D
Zamir
D005638:Fruit / Q000033:anatomy & histology / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D060005:Genotyping Techniques; D018551:Solanum lycopersicum / Q000033:anatomy & histology / Q000235:genetics*; D000069600:Plant Breeding; D019655:Quantitative Trait, Heritable; D032322:Solanum / Q000235:genetics*
D005819:Genetic Markers
Solanum lycopersicum; Solanum pennellii; backcross inbred lines; cuticular waxes; high-resolution mapping; tomato