Community effects in regulation of translation.
Certain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational regulatory defects of the Drosophila oskar (osk) mRNA can be rescued in trans. We proposed that inter-transcript interactions, promoted by assembly of the mRNAs in particles, allow regulatory elements to act in trans. Here we confirm predictions of that model and show that disruption of PTB-dependent particle assembly inhibits rescue in trans. Communication between transcripts is not limited to different osk mRNAs, as regulation imposed by cis-acting elements embedded in the osk mRNA spreads to gurken mRNA. We conclude that community effects exist in translational regulation.
Department of Molecular Biosciences, The University of Texas at Austin, Austin, United States.|Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United States.
Paul M
http://orcid.org/0000-0001-5993-5343
PM
Macdonald
Department of Molecular Biosciences, The University of Texas at Austin, Austin, United States.|Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United States.
Matt
M
Kanke
Department of Molecular Biosciences, The University of Texas at Austin, Austin, United States.|Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United States.
Andrew
A
Kenny
D000818:Animals; D029721:Drosophila Proteins / Q000096:biosynthesis*; D004331:Drosophila melanogaster; D005786:Gene Expression Regulation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*
D029721:Drosophila Proteins; D012333:RNA, Messenger
D. melanogaster; RNP; biochemistry; cell biology; regulation in trans; translation; translational regulation