Effects of light-emitting diodes (LEDs) on the accumulation of lipid content using a two-phase culture process with three microalgae.
Conditions of light-emitting diode (LED) wavelength, light intensity, nitrate concentration, and time of exposure to green LED light stress in a two-phase culture were optimized for lipid production with three species of microalgae. Among the three microalgae, Nannochloropsis oculata showed the highest specific growth rate (μmax) of 0.037h(-1) and the lowest saturation constant (Ks) of 1.32mg/L. The highest lipid contents of the three microalgae in the second phase under green LED light stress were 52.0% (w/w) for Nannochloropsis salina at 2days, 53.0% (w/w) for Nannochloropsis oceanica at 2days, and 56.0% for N. oculata at 2days. Fatty acid analysis of the microalgae showed that 85-87% (w/w) of total fatty acids from N. salina, N. oceanica, and N. oculata consisted of palmitic acid (C16:0) and oleic acid (C18:1).
Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
Chae-Hun
CH
Ra
Busan Techno-park Marine Bio-industry Development Center (MBDC), Busan 46048, Republic of Korea.
Chang-Han
CH
Kang
Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
Jang-Hyun
JH
Jung
Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
Gwi-Taek
GT
Jeong
Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: skkim@pknu.ac.kr.
Sung-Koo
SK
Kim
D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D008027:Light; D008029:Lighting / Q000295:instrumentation*; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D013045:Species Specificity; D058009:Stramenopiles / Q000254:growth & development / Q000378:metabolism*
D005227:Fatty Acids; D008055:Lipids; D009566:Nitrates
Fatty acid; Light-emitting diodes (LEDs); Lipid content; Microalgae; Two-phase culture