Lipin1-Mediated Repression of Adipogenesis by Rutin.
Rutin, also called rutoside or quercetin-3-O-rutinoside and sophorin, is a glycoside between the flavonol quercetin and the disaccharide rutinose. Although many effects of rutin have been reported in vitro and in vivo, the anti-adipogenic effects of rutin have not been fully reported. The aim of this study was to confirm how rutin regulates adipocyte related factors. In this study, rutin decreased the expressions of adipogenesis-related genes, including peroxisome proliferators, activated receptor [Formula: see text] (PPAR[Formula: see text], CCAAT/enhancer-binding protein [Formula: see text] (C/EBP[Formula: see text], fatty acid synthase, adipocyte fatty acid-binding protein, and lipoprotein lipase in 3T3-L1 cells. Rutin also repressed the expression of lipin1, which is an upstream regulator that controls PPAR[Formula: see text] and C/EBP[Formula: see text]. In addition, when 3T3-L1 was transfected with lipin1 siRNA to block lipin1 function, rutin did not affect the expressions of PPAR[Formula: see text] and C/EBP[Formula: see text]. These results suggest that rutin has an anti-adipogenic effect that acts through the suppression of lipin1, as well as PPAR[Formula: see text] and C/EBP[Formula: see text].
* Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Republic of Korea.
Yo-Han
YH
Han
* Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Republic of Korea.
Ji-Ye
JY
Kee
‡ Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Jinbong
J
Park
* Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Republic of Korea.
Dae-Seung
DS
Kim
† Department of Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea.
Soyoung
S
Shin
‡ Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Dong-Hyun
DH
Youn
‡ Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 02447, Republic of Korea.
JongWook
J
Kang
‡ Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Yunu
Y
Jung
* Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Republic of Korea.
Young-Mi
YM
Lee
§ Division of Biotechnology and Convergence, Republic of Korea.
Jin-Han
JH
Park
¶ Department of Cosmeceutical Science, Daegu Hanny University, Yugok-dong, Kyungsan 38578, Republic of Korea.
Su-Jin
SJ
Kim
‡ Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Jae-Young
JY
Um
* Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Republic of Korea.
Seung-Heon
SH
Hong
D016475:3T3 Cells; D055372:AMP-Activated Protein Kinases / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics*; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D051379:Mice; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010711:Phosphatidate Phosphatase / Q000037:antagonists & inhibitors / Q000502:physiology*; D012431:Rutin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D009687:Nuclear Proteins; D047495:PPAR gamma; D012431:Rutin; D055372:AMP-Activated Protein Kinases; C418978:Lpin1 protein, mouse; D010711:Phosphatidate Phosphatase
3T3-L1; Lipin1; Rutin; siRNA