CD38+CD8+ and CD38+CD4+ T Cells and IFN Gamma (+874) Polymorphism Are Associated with a Poor Virological Outcome.
The main objective of the work was to evaluate the use of CD38 on T lymphocytes, IFNγ (+874 A/T), and IL-10 (-1082 A/G) polymorphisms in HIV-infected patients under antiretroviral (ARV) therapy. Sixty-one patients were selected at the outpatient clinic for HIV infection at the Hospital São José de Doenças Infecciosas, Fortaleza, Ceará, Brazil. The patients were classified into two groups, according to viral load after one year of ARV therapy. In the aviremic group (group I), a reduction of 35.5% of CD38+CD4+ T cells was observed (p = 0.02) and 49.3% of CD38+CD8+ T cells (p = 0.001). In the viremic group (group II), a reduction of 37.2% of CD38+CD4+ T cells (p = 0.067), and 21.4% of CD38+CD8+ T cells (p = 0.60) occurred. No association was found between IL-10 (-1082) polymorphism and the type of response to ARV therapy. Regarding the gene polymorphism on IFNγ (+874 T/A), 73.34% of group I and 33.3% of group II presented the AA genotype. The relative risk of the individuals carrying AA genotype or the A allele and not being able to suppress the viral load level after one year of ARV therapy was 3.44 (1.25-9.45; p = 0.014) or 2.35 (1.05-5.26; p = 0.027), respectively. Our data suggested that an augmented frequency of activated CD38+CD8+ T cells as well as the presence of the A allele of IFNγ polymorphism could contribute to a reduced virological suppression in patients under antiretroviral therapy.
a Centro de Ciências da Saúde , Universidade de Fortaleza , Ceará , Brazil.|b Departamento de Análises Clínicas e Toxicológicas , Faculdade de Farmácia, Universidade Federal do Ceará , Fortaleza , Ceará , Brazil.
Paulo Germano
PG
de Carvalho
b Departamento de Análises Clínicas e Toxicológicas , Faculdade de Farmácia, Universidade Federal do Ceará , Fortaleza , Ceará , Brazil.
Raphael
R
de Oliveira Rodrigues
a Centro de Ciências da Saúde , Universidade de Fortaleza , Ceará , Brazil.|c Laboratório de Histocompatibilidade e Imunologia de Transplantes do Centro de Pesquisas em Doenças Hepato Renais, Hemocentro , Fortaleza , Ceará , Brazil.
Silvia Fernandes
SF
Ribeiro da Silva
c Laboratório de Histocompatibilidade e Imunologia de Transplantes do Centro de Pesquisas em Doenças Hepato Renais, Hemocentro , Fortaleza , Ceará , Brazil.
Ilana Farias
IF
Ribeiro
d Centro de Hematologia e de Hemoterapia do Ceará , Fortaleza , Ceará , Brazil.
Herene Barros
HB
de Miranda Lucena
a Centro de Ciências da Saúde , Universidade de Fortaleza , Ceará , Brazil.
Lilian Roberta Costa
LR
Martins
e Departamento de Patologia e de Medicina Legal , Universidade Federal do Ceará , Fortaleza , Ceará , Brazil.
Silvia Helena
SH
Rabenhorst
f Hospital de Doenças Infecciosas São José , Fortaleza , Ceará , Brazil.
Érico Antônio Gomes
ÉA
de Arruda
b Departamento de Análises Clínicas e Toxicológicas , Faculdade de Farmácia, Universidade Federal do Ceará , Fortaleza , Ceará , Brazil.
Aparecida Tiemi
AT
Nagao-Dias
D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000328:Adult; D000368:Aged; D023241:Antiretroviral Therapy, Highly Active; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006678:HIV / Q000502:physiology*; D015658:HIV Infections / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008213:Lymphocyte Activation; D008875:Middle Aged; D011110:Polymorphism, Genetic; D019562:Viral Load
D007371:Interferon-gamma; D051997:ADP-ribosyl Cyclase 1
Activation markers; HIV; cytokine gene polymorphism