The Effects of Topical Application of Polycal (a 2:98 (g/g) Mixture of Polycan and Calcium Gluconate) on Experimental Periodontitis and Alveolar Bone Loss in Rats.
The aim of this study was to observe whether Polycal has inhibitory activity on ligation-induced experimental periodontitis and related alveolar bone loss in rats following topical application to the gingival regions. One day after the ligation placements, Polycal (50, 25, and 12.5 mg/mL solutions at 200 μL/rat) was topically applied to the ligated gingival regions daily for 10 days. Changes in bodyweight, alveolar bone loss index, and total number of buccal gingival aerobic bacterial cells were monitored, and the anti-inflammatory effects were investigated via myeloperoxidase activity and levels of the pro-inflammatory cytokines IL-1β and TNF-α. The activities of inducible nitric oxide synthase (iNOS) and lipid peroxidation (MDA) were also evaluated. Bacterial proliferation, periodontitis, and alveolar bone loss induced by ligature placements were significantly inhibited after 10 days of continuous topical application of Polycal. These results indicate that topical application of Polycal has a significant inhibitory effect on periodontitis and related alveolar bone loss in rats mediated by antibacterial, anti-inflammatory, and anti-oxidative activities.
The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Gyeongsan 38610, Korea. sangin0118@naver.com.|Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. sangin0118@naver.com.
Sang-In
SI
Park
The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Gyeongsan 38610, Korea. vegonia1@hanmail.net.|Department of Preventive Medicine, College of Korean Medicine, Deagu Haany University, Gyeongsan 38610, Korea. vegonia1@hanmail.net.
Su-Jin
SJ
Kang
Clinical Research Division, Korean Institute of Oriental Medicine, Daejeon 34054, Korea. chhan@kiom.re.kr.
Chang-Hyun
CH
Han
ARIBIO, Jecheon 27159, Korea. kimjw@aribio.com.
Joo-Wan
JW
Kim
The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Gyeongsan 38610, Korea. dvmsong@hotmail.com.|Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. dvmsong@hotmail.com.
Chang-Hyun
CH
Song
Department of Qigong, College of Korean Medicine, Deagu Haany University, Gyeongsan 38610, Korea. lsn1974@dhu.ac.kr.
Sang-Nam
SN
Lee
The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Gyeongsan 38610, Korea. gucci200@hanmail.net.|Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea. gucci200@hanmail.net.
Sae-Kwang
SK
Ku
The Medical Research Center for Globalization of Herbal Medicine, Daegu Haany University, Gyeongsan 38610, Korea. gksxntk@dhu.ac.kr.|Department of Preventive Medicine, College of Korean Medicine, Deagu Haany University, Gyeongsan 38610, Korea. gksxntk@dhu.ac.kr.
Young-Joon
YJ
Lee
D000287:Administration, Topical; D016301:Alveolar Bone Loss / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002125:Calcium Gluconate / Q000008:administration & dosage* / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004338:Drug Combinations; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010518:Periodontitis / Q000188:drug therapy* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D047071:beta-Glucans / Q000008:administration & dosage* / Q000494:pharmacology
D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004338:Drug Combinations; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; D002125:Calcium Gluconate
alveolar bone loss; anti-bacterial effects; anti-inflammatory; antioxidant; calcium gluconate; β-glucan