Piperlongumine Suppresses Proliferation of Human Oral Squamous Cell Carcinoma through Cell Cycle Arrest, Apoptosis and Senescence.
Oral squamous cell carcinoma (OSCC), an aggressive cancer originating in the oral cavity, is one of the leading causes of cancer deaths in males worldwide. This study investigated the antitumor activity and mechanisms of piperlongumine (PL), a natural compound isolated from Piper longum L., in human OSCC cells. The effects of PL on cell proliferation, the cell cycle, apoptosis, senescence and reactive oxygen species (ROS) levels in human OSCC cells were investigated. PL effectively inhibited cell growth, caused cell cycle arrest and induced apoptosis and senescence in OSCC cells. Moreover, PL-mediated anti-human OSCC behavior was inhibited by an ROS scavenger N-acetyl-l-cysteine (NAC) treatment, suggesting that regulation of ROS was involved in the mechanism of the anticancer activity of PL. These findings suggest that PL suppresses tumor growth by regulating the cell cycle and inducing apoptosis and senescence and is a potential chemotherapy agent for human OSCC cells.
Department of Chinese Medicine, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi 600, Taiwan. cych02157@gmail.com.|Department of Life Science, National Chung Cheng University, Chiayi 621, Taiwan. cych02157@gmail.com.
San-Yuan
SY
Chen
Department of Life Science, National Chung Cheng University, Chiayi 621, Taiwan. ai90395@gmail.com.
Geng-Hung
GH
Liu
Department of Nursing, Min-Hwei Junior College of Health Care Management, Tainan City 736, Taiwan. april@mail.mhchcm.edu.tw.
Wen-Ying
WY
Chao
Graduate Institute of Clinical Medical Sciences, Chang Gung University, Chiayi 613, Taiwan. csshi@mail.cgu.edu.tw.
Chung-Sheng
CS
Shi
Department of Medical Research, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi 600, Taiwan. jouyuan22@gmail.com.
Ching-Yen
CY
Lin
Department of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. limyp@mail.cmu.edu.tw.
Yun-Ping
YP
Lim
Division of Endocrinology and Metabolism, Department of Internal Medicine, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi 600, Taiwan. 02602@cych.org.tw.
Chieh-Hsiang
CH
Lu
Department of Life Science, National Chung Cheng University, Chiayi 621, Taiwan. laiweichien@yahoo.com.tw.
Peng-Yeh
PY
Lai
Department of Life Science, National Chung Cheng University, Chiayi 621, Taiwan. biohrc@ccu.edu.tw.
Hau-Ren
HR
Chen
Department of Nursing, Min-Hwei Junior College of Health Care Management, Tainan City 736, Taiwan. yingray.lee@gmail.com.|Department of Medical Research, Ditmanson Medical Foundation Chiayi Christian Hospital, Chiayi 600, Taiwan. yingray.lee@gmail.com.
Ying-Ray
YR
Lee
D000111:Acetylcysteine / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004148:Dioxolanes / Q000494:pharmacology*; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D010881:Piperidones
D000111:Acetylcysteine; D000970:Antineoplastic Agents; D053148:Caspase 3; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004148:Dioxolanes; D017382:Reactive Oxygen Species; C498077:piperlongumine; D010881:Piperidones
apoptosis; cell cycle arrest; human oral squamous cell carcinoma; piperlongumine; senescence