Enhancing resolution of free-flow zone electrophoresis via a simple sheath-flow sample injection.
In this work, a simple and novel sheath-flow sample injection method (SFSIM) is introduced to reduce the band broadening of free-flow zone electrophoresis separation in newly developed self-balance free-flow electrophoresis instrument. A needle injector was placed in the center of the separation inlet, into which the BGE and sample solution were pumped simultaneously. BGE formed sheath flow outside the sample stream, resulting in less band broadening related to hydrodynamics and electrodynamics. Hemoglobin and C-phycocyanin were successfully separated by the proposed method in contrast to the poor separation of free-flow electrophoresis with the traditional injection method without sheath flow. About 3.75 times resolution enhancement could be achieved by sheath-flow sample injection method.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China.
Ying
Y
Yang
Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Fan-Zhi
FZ
Kong
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China.
Ji
J
Liu
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China.
Jun-Min
JM
Li
Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Xiao-Ping
XP
Liu
Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Guo-Qing
GQ
Li
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China.
Ju-Fang
JF
Wang
Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Hua
H
Xiao
Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Liu-Yin
LY
Fan
Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Cheng-Xi
CX
Cao
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China.
Shan
S
Li
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D009339:Needles; D011506:Proteins / Q000302:isolation & purification
D011506:Proteins
Band broadening; Free-flow zone electrophoresis; Sample injection; Sheath flow