Molecular dissection of Oryza sativa salt-induced RING Finger Protein 1 (OsSIRP1): possible involvement in the sensitivity response to salinity stress.
Ubiquitination-mediated protein degradation via Really Interesting New Gene (RING) E3 ligase plays an important role in plant responses to abiotic stress conditions. Many plant studies have found that RING proteins regulate the perception of various abiotic stresses and signal transduction. In this study, Oryza sativa salt-induced RING Finger Protein 1 (OsSIRP1) gene was selected randomly from 44 Oryza sativa RING Finger Proteins (OsRFPs) genes highly expressed in rice roots exposed to salinity stress. Transcript levels of OsSIRP1 in rice leaves after various stress treatments, including salt, heat, drought and hormone abscisic acid (ABA), were observed. Poly-ubiquitinated products of OsSIRP1 were investigated via an in vitro ubiquitination assay.35S:OsSIRP1-EYFP was distributed in the cytosol of untreated and salt-treated rice protoplasts. Heterogeneous overexpression of OsSIRP1 in Arabidopsis reduced tolerance for salinity stress during seed germination and root growth. Our findings indicate that OsSIRP1 acts as a negative regulator of salinity stress tolerance mediated by the ubiquitin 26S proteasome system.
Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Korea.|Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon, 200-713, Korea.
Sun-Goo
SG
Hwang
Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Korea.
Jung Ju
JJ
Kim
Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Korea.
Sung Don
SD
Lim
Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Korea.
Yong Chan
YC
Park
Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Korea.|Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon, 200-713, Korea.
Jun-Cheol
JC
Moon
Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Korea. csjang@kangwon.ac.kr.
Cheol Seong
CS
Jang
D000040:Abscisic Acid / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D012275:Oryza / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000502:physiology; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D054712:Salinity; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D054875:Ubiquitination
D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D012965:Sodium Chloride; D000040:Abscisic Acid; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease