Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology.
Transformation-associated recombination (TAR) cloning represents a unique tool for isolation and manipulation of large DNA molecules. The technique exploits a high level of homologous recombination in the yeast Sacharomyces cerevisiae. So far, TAR cloning is the only method available to selectively recover chromosomal segments up to 300 kb in length from complex and simple genomes. In addition, TAR cloning allows the assembly and cloning of entire microbe genomes up to several Mb as well as engineering of large metabolic pathways. In this review, we summarize applications of TAR cloning for functional/structural genomics and synthetic biology.
Developmental Therapeutics Branch, National Cancer Institute, Bethesda, MD, 20892, USA. kouprinn@mail.nih.gov.
Natalay
N
Kouprina
Developmental Therapeutics Branch, National Cancer Institute, Bethesda, MD, 20892, USA.
Vladimir
V
Larionov
D000483:Alleles; D022222:Chromosomes, Artificial, Human / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D010963:Plasmodium falciparum / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
HAC; Human artificial chromosome; Synthetic biology; TAR cloning; Transformation-associated recombination