Determination of Six Pyrazole Fungicides in Grape Wine by Solid-Phase Extraction and Gas Chromatography-Tandem Mass Spectrometry.
A gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed for the first simultaneous identification and quantification of six pyrazole fungicides (furametpyr, rabenzazole, fluxapyroxad, penflufen, bixafen, and isopyrazam) in grape wine samples. The grape wine samples were first diluted with water, then purified by solid-phase extraction, and finally examined by GC-MS/MS in multiple reaction monitoring (MRM) mode. Matrix-matched calibration curves were used to correct the matrix effects. The limits of quantification (LOQs), calculated as 10 times the standard deviation, were 0.2-0.8 μg kg(-1) for the six pyrazole fungicides. The average recoveries were in the range of 74.3-94.5%, with relative standard deviations (RSDs) below 5.8%, measured at three concentration levels. The proposed method is suitable for the simultaneous determination of six pyrazole fungicides in grape wine samples.
College of Chemistry and Materials Engineering, Wenzhou University , Wenzhou, Zhejiang 325035, People's Republic of China.
Yan
Y
Shen
Wenzhou Entry-Exit Inspection and Quarantine Bureau of People's Republic of China , Wenzhou, Zhejiang 325027, People's Republic of China.
Zhou
Z
Li
Chinese Academy of Inspection and Quarantine , Beijing 100176, People's Republic of China.
Qiang
Q
Ma
Shanghai Entry-Exit Inspection and Quarantine Bureau of People's Republic of China , Shanghai 200135, People's Republic of China.
Chuanxian
C
Wang
Wenzhou Entry-Exit Inspection and Quarantine Bureau of People's Republic of China , Wenzhou, Zhejiang 325027, People's Republic of China.
Xiangzhun
X
Chen
College of Chemistry and Materials Engineering, Wenzhou University , Wenzhou, Zhejiang 325035, People's Republic of China.
Qian
Q
Miao
Wenzhou Entry-Exit Inspection and Quarantine Bureau of People's Republic of China , Wenzhou, Zhejiang 325027, People's Republic of China.
Chao
C
Han
D005659:Fungicides, Industrial / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D011720:Pyrazoles / Q000032:analysis*; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
D005659:Fungicides, Industrial; D011720:Pyrazoles
GC−MS/MS; SPE; grape wines; pyrazole fungicides