In Vitro Behavior of Osteoblasts on Zirconia After Different Intensities of Erbium, Chromium-Doped: Yttrium, Scandium, Gallium, and Garnet-Laser Irradiation.
OBJECTIVE
This study investigated the osteoblastic behavior to yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP) surface after different intensities of erbium, chromium-doped:yttrium, scandium, gallium, and garnet (Er,Cr:YSGG) laser irradiation treatment.


METHODS
Y-TZP blocks were randomized divided into 4 groups to Er,Cr:YSGG laser irradiation: group G1 = control (no laser irradiation); group G2 = 1.5 W; group G3 = 3.0 W; group G4 = 5.0 W, with air-water irrigation. The irradiated surface roughness (Sa and Ra) topography was evaluated. MC3T3-E1 osteoblast cells were cultured and seeded onto the treated surfaces at a plate density of 1 × 10 cells/mL. At the third and seventh days cell proliferation was measured by the colorimetric assay methods. The specimens were prepared to scanning electron microscope to observe adhesion and morphology of cells.


RESULTS
MTT assay showed adhesion and proliferation of MC3T3-E1 cells in all groups. Compared with group G1 (control), the group G2 had a proliferation 1.4% higher (P > 0.05), the group G3 had a proliferation 3.1% higher (P < 0.05) and group G4 had a proliferation 4.5% higher (P < 0.01). After 7 days there was no difference among the groups (ANOVA, Newman-Keuls).


CONCLUSIONS
Er,Cr:YSGG laser irradiation may have contributed to the greater and earlier cell spreading that was observed with the Y-TZP without alterations on surface topography.
*Department of Periodontology and Oral Implantology†Department of Restorative Dentistry, Dental Research Division, University of Guarulhos, Guarulhos‡Master of Science Program in Clinical Dentistry, Positivo University, Curitiba§Federal University of Santa Catarina, Florianopolis||Biotecnos-Technology and Science, Santa Maria¶Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos, Guarulhos#Private Practice**Department of Dental Materials, University of São Paulo, São Paulo††Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos, Guarulhos, Brazil.
Renan D
RD
Soares
José A
JA
Rodrigues
Alessandra
A
Cassoni
Ariadne
A
Cruz
Claudia O
CO
Simões
Jose D
JD
Pasqua-Neto
Sergio Alexandre
SA
Gehrke
Alberto
A
Blay
Paulo F
PF
Cesar
Jamil Awad
JA
Shibli
D002857:Chromium; D004305:Dose-Response Relationship, Drug; D004871:Erbium; D005708:Gallium; D006801:Humans; D066298:In Vitro Techniques; D053844:Lasers, Solid-State / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000528:radiation effects*; D012538:Scandium; D013499:Surface Properties; D015019:Yttrium; D015040:Zirconium
D002857:Chromium; D015019:Yttrium; D004871:Erbium; D015040:Zirconium; D005708:Gallium; C028541:zirconium oxide; D012538:Scandium