An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods?
The free radical nitric oxide (NO) is a universal signaling molecule among living organisms. To investigate versatile functions of NO in plants it is essential to analyze biologically produced NO with an appropriate method. Owing to the uniqueness of NO, plant researchers may encounter difficulties in applying methods that have been developed for mammalian study. Based on our experience, we present here a practical guide to NO measurement fitted to plant biology.
Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan. yamasaki@sci.u-ryukyu.ac.jp.
Hideo
H
Yamasaki
Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan.
Naoko S
NS
Watanabe
Research Faculty of Agriculture, Hokkaido University, Kita Ku, Kita 9, Nishi 9, Sapporo, Hokkaido, 060-8589, Japan.
Yasuko
Y
Sakihama
Department of Biology, Sonoma State University, Rohnert Park, CA, 94928, USA.|Biological Systems Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna-son, Kunigami-gun, Okinawa, 904-0495, Japan.
Michael F
MF
Cohen
D049449:Luminescence; D009569:Nitric Oxide / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*
D009569:Nitric Oxide
Chemiluminescence detection; DAF; Electrochemical detection; Nitric oxide; RNS; ROS; RSS; cPTIO