Characterization of biosurfactants produced by Lactobacillus spp. and their activity against oral streptococci biofilm.
Lactic acid bacteria (LAB) can interfere with pathogens through different mechanisms; one is the production of biosurfactants, a group of surface-active molecules, which inhibit the growth of potential pathogens. In the present study, biosurfactants produced by Lactobacillus reuteri DSM 17938, Lactobacillus acidophilus DDS-1, Lactobacillus rhamnosus ATCC 53103, and Lactobacillus paracasei B21060 were dialyzed (1 and 6 kDa) and characterized in term of reduction of surface tension and emulsifying activity. Then, aliquots of the different dialyzed biosurfactants were added to Streptococcus mutans ATCC 25175 and Streptococcus oralis ATCC 9811 in the culture medium during the formation of biofilm on titanium surface and the efficacy was determined by agar plate count, biomass analyses, and flow cytometry. Dialyzed biosurfactants showed abilities to reduce surface tension and to emulsifying paraffin oil. Moreover, they significantly inhibited the adhesion and biofilm formation on titanium surface of S. mutans and S. oralis in a dose-dependent way, as demonstrated by the remarkable decrease of cfu/ml values and biomass production. The antimicrobial properties observed for dialyzed biosurfactants produced by the tested lactobacilli opens future prospects for their use against microorganisms responsible of oral diseases.
Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy.
Eleonora
E
Ciandrini
Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy.
Raffaella
R
Campana
Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy.
Luca
L
Casettari
School of Pharmacy, University of Camerino, via S. Agostino 1, 62032, Camerino, MC, Italy.
Diego R
DR
Perinelli
Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy.
Laura
L
Fagioli
Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy.
Anita
A
Manti
School of Pharmacy, University of Camerino, via S. Agostino 1, 62032, Camerino, MC, Italy.
Giovanni Filippo
GF
Palmieri
Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy.
Stefano
S
Papa
Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy. wally.baffone@uniurb.it.
Wally
W
Baffone
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000254:growth & development*; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D000069980:Lacticaseibacillus paracasei / Q000378:metabolism*; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development*; D019533:Streptococcus oralis / Q000187:drug effects / Q000254:growth & development*; D013500:Surface Tension / Q000187:drug effects; D013501:Surface-Active Agents / Q000378:metabolism* / Q000494:pharmacology; D014025:Titanium
D000900:Anti-Bacterial Agents; D013501:Surface-Active Agents; D014025:Titanium
Biofilm formation percentages; Biosurfactants; Chemical characterization; Lactobacillus; Oral streptococci