Propagation and Separation of Charged Colloids by Cylindrical Passivated Gel Electrophoresis.
We explore the electrophoretic propagation of charged colloidal objects, monodisperse anionically stabilized polystyrene spheres, in large-pore agarose gels that have been passivated using polyethylene glycol (PEG) when a radial electric field is applied in a cylindrical geometry. By contrast to standard Cartesian gel-electrophoresis geometries, in a cylindrical geometry, charged particles that start at a ring well near the central axis propagate outward more rapidly initially and then slow down as they move further away from the axis. By building a full-ring cylindrical gel electrophoresis chamber and taking movies of scattered light from propagating nanospheres undergoing electrophoresis, we experimentally demonstrate that the ring-like front of monodisperse nanospheres propagates stably in PEG-passivated agarose gels and that the measured ring radius as a function of time agrees with a simple model that incorporates the electric field of a cylindrical geometry. Moreover, we show that this cylindrical geometry offers a potential advantage when performing electrophoretic separations of objects that have widely different sizes: smaller objects can still be retained in a cylindrical gel that has a limited size over long electrophoretic run times required for separating larger objects.
Department of Chemistry and Biochemistry, University of California-Los Angeles , Los Angeles, California 90095, United States.
Dimitri
D
Bikos
Department of Chemistry and Biochemistry, University of California-Los Angeles , Los Angeles, California 90095, United States.|Department of Physics and Astronomy, University of California-Los Angeles , Los Angeles, California 90095, United States.
Thomas G
TG
Mason