Suppressing the cytotoxicity of CuO nanoparticles by uptake of curcumin/BSA particles.
The adverse effects of metal-based nanoparticles on human beings and the environment have received extensive attention recently. It is urgently required to develop a simple and effective method to suppress the toxicity of metal-based nanomaterials. In this study, a hydrophobic antioxidant and a chelation agent curcumin (CUR) were encapsulated into bovine serum albumin (BSA) particles by a simple co-precipitation method, and followed by glutaraldehyde cross-linking. The CUR/BSA particles had an average size of 300 nm in diameter with a negatively charged surface and sustained curcumin release properties. The cellular uptake and cytotoxicity of CUR/BSA particles were followed on A549 cells, HepG2 cells and RAW264.7 cells. The CUR/BSA particles had higher intracellular accumulation and lower cytotoxicity compared with the free curcumin at the same drug concentration. The CUR/BSA particles could suppress the cytotoxicity generated by CuO nanoparticles as a result of decrease of both the intracellular reactive oxygen species (ROS) level and Cu(2+) concentration, while the free curcumin did not show any obvious detoxicating effect. The detoxicating effects of CUR/BSA particles were further studied in an intratracheal instillation model in vivo, demonstrating significant reduction of toxicity and inflammatory response in rat lungs induced by CuO nanoparticles. The concept-proving study demonstrates the potential of the CUR/BSA particles in suppressing cytotoxicity of metal-based nanomaterials, which is a paramount requirement for the safe application of nanotechnology.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. zwmao@zju.edu.cn cygao@mail.hz.zj.cn.
Wenjing
W
Zhang
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. zwmao@zju.edu.cn cygao@mail.hz.zj.cn.
Pengfei
P
Jiang
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. zwmao@zju.edu.cn cygao@mail.hz.zj.cn.
Ying
Y
Chen
Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China.
Peihua
P
Luo
Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China.
Guanqun
G
Li
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. zwmao@zju.edu.cn cygao@mail.hz.zj.cn.
Botuo
B
Zheng
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. zwmao@zju.edu.cn cygao@mail.hz.zj.cn.
Wei
W
Chen
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. zwmao@zju.edu.cn cygao@mail.hz.zj.cn.
Zhengwei
Z
Mao
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. zwmao@zju.edu.cn cygao@mail.hz.zj.cn.
Changyou
C
Gao
D000072283:A549 Cells; D000818:Animals; D000975:Antioxidants; D003300:Copper / Q000633:toxicity*; D003474:Curcumin / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D000067996:RAW 264.7 Cells; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism
D000975:Antioxidants; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D003300:Copper; D003474:Curcumin