Scaffold protein enigma homolog 1 overcomes the repression of myogenesis activation by inhibitor of DNA binding 2.
Enigma Homolog 1 (ENH1) is a scaffold protein for signaling proteins and transcription factors. Previously, we reported that ENH1 overexpression promotes the differentiation of C2C12 myoblasts. However, the molecular mechanism underlying the role of ENH1 in the C2C12 cells differentiation remains elusive. ENH1 was shown to inhibit the proliferation of neuroblastoma cells by sequestering Inhibitor of DNA binding protein 2 (Id2) in the cytosol. Id2 is a repressor of basic Helix-Loop-Helix transcription factors activity and prevents myogenesis. Here, we found that ENH1 overcome the Id2 repression of C2C12 cells myogenic differentiation and that ENH1 overexpression promotes mice satellite cells activation, the first step toward myogenic differentiation. In addition, we show that ENH1 interacted with Id2 in C2C12 cells and mice satellite cells. Collectively, our results suggest that ENH1 plays an important role in the activation of myogenesis through the repression of Id2 activity.
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Nagoya, 464-8106, Japan.
Miyuki
M
Nakatani
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Nagoya, 464-8106, Japan; Japan Society for the Promotion of Science, Tokyo, 102-0083, Japan.
Jumpei
J
Ito
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Nagoya, 464-8106, Japan.
Riko
R
Koyama
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka, 567-0047, Japan.
Masumi
M
Iijima
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka, 567-0047, Japan.
Nobuo
N
Yoshimoto
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Nagoya, 464-8106, Japan.
Tomoaki
T
Niimi
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Nagoya, 464-8106, Japan; The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka, 567-0047, Japan.
Shun'ichi
S
Kuroda
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Nagoya, 464-8106, Japan. Electronic address: maturana@agr.nagoya-u.ac.jp.
Andrés D
AD
Maturana
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D051798:Inhibitor of Differentiation Protein 1 / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D024510:Muscle Development / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism*; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; C510085:ENH protein, mouse; C497455:Idb1 protein, mouse; D051798:Inhibitor of Differentiation Protein 1; D008840:Microfilament Proteins; D034521:Nuclear Matrix-Associated Proteins
C2C12 cells; Myogenesis; Scaffold protein