Effective molarity in a nucleic acid-controlled reaction.
Positioning of reactive functional groups within a DNA duplex can enable chemical reactions that otherwise would not occur to an appreciable extent. However, few studies have quantitatively defined the extent to which the enforced proximity of reaction partners in duplex DNA can favor chemical processes. Here, we measured substantial effective molarities (as high as 25M) afforded by duplex DNA to a reaction involving interstrand cross-link formation between 2'-deoxyadenosine and a 2-deoxyribose abasic (Ap) site.
University of Missouri, Department of Chemistry, 125 Chemistry Building, Columbia, MO 65211, United States.
Michael J
MJ
Catalano
University of Missouri, Department of Chemistry, 125 Chemistry Building, Columbia, MO 65211, United States.
Nathan E
NE
Price
University of Missouri, Department of Chemistry, 125 Chemistry Building, Columbia, MO 65211, United States; University of Missouri, Department of Biochemistry, 125 Chemistry Building, Columbia, MO 65211, United States. Electronic address: gatesk@missouri.edu.
Kent S
KS
Gates
D004247:DNA / Q000737:chemistry*; D003839:Deoxyadenosines / Q000737:chemistry*; D003855:Deoxyribose / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D013329:Structure-Activity Relationship
D003839:Deoxyadenosines; D003855:Deoxyribose; D004247:DNA; C058118:2'-deoxyadenosine
Abasic site; DNA alkylation; DNA cross-link; Effective molarity; Imine