Boosting TAG Accumulation with Improved Biodiesel Production from Novel Oleaginous Microalgae Scenedesmus sp. IITRIND2 Utilizing Waste Sugarcane Bagasse Aqueous Extract (SBAE).
This investigation utilized sugarcane bagasse aqueous extract (SBAE), a nontoxic, cost-effective medium to boost triacylglycerol (TAG) accumulation in novel fresh water microalgal isolate Scenedesmus sp. IITRIND2. Maximum lipid productivity of 112 ± 5.2 mg/L/day was recorded in microalgae grown in SBAE compared to modified BBM (26 ± 3 %). Carotenoid to chlorophyll ratio was 12.5 ± 2 % higher than in photoautotrophic control, indicating an increase in photosystem II activity, thereby increasing growth rate. Fatty acid methyl ester (FAME) profile revealed presence of C14:0 (2.29 %), C16:0 (15.99 %), C16:2 (4.05 %), C18:0 (3.41 %), C18:1 (41.55 %), C18:2 (12.41), and C20:0 (1.21 %) as the major fatty acids. Cetane number (64.03), cold filter plugging property (-1.05 °C), and oxidative stability (12.03 h) indicated quality biodiesel abiding by ASTM D6751 and EN 14214 fuel standards. Results consolidate the candidature of novel freshwater microalgal isolate Scenedesmus sp. IITRIND2 cultivated in SBAE, aqueous extract made from copious, agricultural waste sugarcane bagasse to increase the lipid productivity, and could further be utilized for cost-effective biodiesel production.
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India.
Neha
N
Arora
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India.
Alok
A
Patel
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India.
Parul A
PA
Pruthi
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India. vikasfbs@gmail.com.
Vikas
V
Pruthi
D052818:Autotrophic Processes / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry*; D004952:Esters / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D058086:Microalgae / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010936:Plant Extracts / Q000494:pharmacology; D012015:Reference Standards; D031786:Saccharum / Q000737:chemistry*; D044444:Scenedesmus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D014280:Triglycerides / Q000378:metabolism*; D014866:Waste Products
D056804:Biofuels; D004952:Esters; D008055:Lipids; D010936:Plant Extracts; D014280:Triglycerides; D014866:Waste Products; D002244:Carbon; D002482:Cellulose; C027433:bagasse
Biodiesel; Lipid productivity; Scenedesmus sp. IITRIND2; Sugarcane bagasse; Triacylglycerol