Characterisation of potential novel allergens in the fish parasiteAnisakis simplex.
The parasitic nematodeAnisakis simplexoccurs in fish stocks in temperate seas.A. simplexcontamination of fish products is unsavoury and a health concern considering human infection with live larvae (anisakiasis) and allergic reactions to anisakid proteins in seafood. Protein extracts ofA. simplexproduce complex band patterns in gel electrophoresis and IgE-immunostaining. In the present study potential allergens have been characterised using sera fromA. simplex-sensitised patients and proteome data obtained by mass spectrometry.A. simplexproteins were homologous to allergens in other nematodes, insects, and shellfish indicating cross-reactivity. Characteristic marker peptides for relevantA. simplexproteins were described.
Norwegian Veterinary Institute, Oslo, Norway.
Christiane Kruse
CK
Fæste
University of Colorado Denver, Aurora, CO, USA.
Karen R
KR
Jonscher
Norwegian Veterinary Institute, Oslo, Norway.
Maaike M W B
MM
Dooper
University of Oslo, Oslo, Norway.
Wolfgang
W
Egge-Jacobsen
University of Oslo, Oslo, Norway.
Anders
A
Moen
Instituto de Investigación Sanitaria-Hospital Universitario de La Princesa, Madrid, Spain.
Alvaro
A
Daschner
Norwegian Veterinary Institute, Oslo, Norway.
Eliann
E
Egaas
University of Colorado Denver, Aurora, CO, USA.
Uwe
U
Christians
Allergenicity; Anisakis simplex; Cross-reactivity; Immunoblotting; Mass spectrometry; Proteomic analysis