Basal neutrophil function in human aging: Implications in endothelial cell adhesion.
Much attention has been drawn to the pro-inflammatory condition that accompanies aging. This study compared parameters from non-stimulated neutrophils, obtained from young (18-30 years old [y.o.]) and elderly (65-80 y.o.) human volunteers. Measured as an inflammatory marker, plasmatic concentration of hs-CRP was found higher in elderly individuals. Non-stimulated neutrophil production of ROS and NO was, respectively, 38 and 29% higher for the aged group. From the adhesion molecules evaluated, only CD11b expression was elevated in neutrophils from the aged group, whereas no differences were found for CD11a, CD18, or CD62. A 69% higher non-stimulated in vitro neutrophil/endothelial cell adhesion was observed for neutrophils isolated from elderly donors. Our results suggest that with aging, neutrophils may be constitutively producing more reactive species in closer proximity to endothelial cells of vessel walls, which may both contribute to vascular damage and reflect a neutrophil intracellular disrupted redox balance, altering neutrophil function in aging.
Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil.
Joes
J
Nogueira-Neto
Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil.
André S C
AS
Cardoso
Department of Biochemistry/Molecular Biology, Federal University of São Paulo, São Paulo, SP, Brazil.
Hugo P
HP
Monteiro
Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil.|Department of Hematology and Oncology, ABC School of Medicine, Santo André, SP, Brazil.
Fernando L A
FL
Fonseca
Department of Preventive Medicine, Federal University of São Paulo, São Paulo, SP, Brazil.
Luiz Roberto
LR
Ramos
Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil.
Virginia B C
VB
Junqueira
Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil.|Laboratório de Análises Clínicas e Toxicológicas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua São Nicolau, 210-1° Andar, Diadema, 09913-030, SP, Brazil.
Karin A
KA
Simon
D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging / Q000502:physiology; D015703:Antigens, CD / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D015703:Antigens, CD; D039481:CD11b Antigen; D015815:Cell Adhesion Molecules; C509295:ITGAM protein, human; D017382:Reactive Oxygen Species; D002097:C-Reactive Protein
aging; cell function; endothelial implications