Karyotype analysis of the acute fibrosarcoma from chickens infected with subgroup J avian leukosis virus associated with v-src oncogene.
To understand the cytogenetic characteristics of acute fibrosarcoma in chickens infected with the subgroup J avian leukosis virus associated with the v-src oncogene, we performed a karyotype analysis of fibrosarcoma cell cultures. Twenty-nine of 50 qualified cell culture spreads demonstrated polyploidy of some macrochromosomes, 21 of which were trisomic for chromosome 7, and others were trisomic for chromosomes 3, 4, 5 (sex chromosome w), and 10. In addition, one of them was trisomic for both chromosome 7 and the sex chromosome 5 (w). In contrast, no aneuploidy was found for 10 macrochromosomes of 12 spreads of normal chicken embryo fibroblast cells, although aneuploidy for some microchromosomes was demonstrated in five of the 12 spreads. The cytogenetic mosaicism or polymorphism of the aneuploidy in the acute fibrosarcoma described in this study suggests that the analysed cells are polyclonal.
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Xuan
X
Dong
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Sidi
S
Ju
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Junxia
J
Chen
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Fanfeng
F
Meng
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Peng
P
Sun
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Yang
Y
Li
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Xin
X
Wang
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Yixin
Y
Wang
b Qingdao Entry-Exit Inspection and Quarantine Bureau , Qingdao , People's Republic of China.
Juan
J
Liu
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Shuang
S
Chang
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Peng
P
Zhao
a College of Veterinary Medicine, Shandong Agricultural University , Taian , People's Republic of China.
Zhizhong
Z
Cui
D000208:Acute Disease; D000818:Animals; D001353:Avian Leukosis / Q000235:genetics / Q000821:virology*; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000302:isolation & purification; D002642:Chick Embryo; D002645:Chickens / Q000821:virology*; D002869:Chromosome Aberrations; D005260:Female; D005354:Fibrosarcoma / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D016391:Genes, src / Q000235:genetics*; D059785:Karyotype; D007621:Karyotyping / Q000662:veterinary; D011110:Polymorphism, Genetic
Karyotype analysis; acute fibrosarcoma; subgroup J avian leukosis virus; v-src