Identification of the differentially accumulated proteins associated with low phosphorus tolerance in a Tibetan wild barley accession.
Low phosphorus (LP) in soil is a widely-occurred limiting factor for crop production in the world. In a previous study we identified a highly LP-tolerant Tibetan wild barley accession (XZ99). Here, a comparatively proteomic analysis was conducted using three barley genotypes differing in LP tolerance to reveal the mechanisms underlying the LP tolerance of XZ99. Totally, 31 differentially accumulated proteins were identified in the roots and leaves of the three genotypes using 2-dimensional gel electrophoresis coupled with mass spectrometry. They were involved in the various biological processes, including carbon and energy metabolism, signal transduction, cell growth and division, secondary metabolism, and stress defense. In comparison with XZ100 (LP sensitive) and ZD9 (LP moderately-tolerant), XZ99 had a more developed root system, which is mainly attributed to enhanced carbohydrate metabolizing proteins under LP conditions. The current results showed that Tibetan wild barley XZ99 and cultivated barley cultivar ZD9 differ in the mechanism of LP tolerance. The changes of the proteins associated with carbohydrate metabolism could account for the difference between the LP-tolerant and LP-sensitive genotypes. In addition, the mRNA expression levels of 9 LP responsive proteins were verified by qRT-PCR. The current results may open a new avenue of understanding the LP tolerance in plants on the proteomic basis.
Institute of Crop Science, Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
Umme Aktari
UA
Nadira
Institute of Crop Science, Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
Imrul Mosaddek
IM
Ahmed
Institute of Crop Science, Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
Jianbin
J
Zeng
Institute of Crop Science, Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
Feibo
F
Wu
Institute of Crop Science, Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China. Electronic address: zhanggp@zju.edu.cn.
Guoping
G
Zhang
D000222:Adaptation, Physiological / Q000187:drug effects*; D002241:Carbohydrates / Q000032:analysis; D060146:Ecotype; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005838:Genotype; D001467:Hordeum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010641:Phenotype; D010758:Phosphorus / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics
D002241:Carbohydrates; D010940:Plant Proteins; D012333:RNA, Messenger; D010758:Phosphorus
2-DE (2-dimensional gel electrophoresis); Barley (Hordeum vulgare L.); Low phosphorus; Proteomics analysis; Tolerance