The effects of NONRATT021972 lncRNA siRNA on PC12 neuronal injury mediated by P2X7 receptor after exposure to oxygen-glucose deprivation.
Adenosine triphosphate (ATP) participates in signal transmission by acting on P2X receptors, and the P2X7 receptor is involved in the pathophysiological changes of ischemic injury. The PC12 cell line is a popular model system to study sympathetic neuronal function. Long noncoding RNAs (lncRNAs) are highly expressed in the nervous system and serve as regulatory RNAs. In this study, the effects of NONRATT021972 lncRNA siRNA on P2X7-mediated PC12 neuronal injury after exposure to oxygen-glucose deprivation (OGD) were investigated. Our results showed that the viability of PC12 cells cultured with OGD or the P2X7 agonist BzATP was significantly decreased. Treatment with NONRATT021972 siRNA reversed the decreased viability of PC12 cells under OGD conditions. The upregulated P2X7 mRNA and protein levels in PC12 cells under OGD conditions or BzATP treatment were significantly decreased when pretreated with NONRATT021972 siRNA. Moreover, NONRATT021972 siRNA treatment effectively suppressed the increase in [Ca(2+)]i induced by OGD or P2X7 agonists (ATP or BzATP) in PC12 cells. Therefore, treatment with NONRATT021972 siRNA may decrease sympathetic neuronal injury induced by ischemia.
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Guilin
G
Li
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Lifang
L
Zou
Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Wei
W
Xie
Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Shiyao
S
Wen
Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Qiuyu
Q
Xie
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Yun
Y
Gao
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Changshui
C
Xu
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Hong
H
Xu
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Shuangmei
S
Liu
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Shouyu
S
Wang
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Yun
Y
Xue
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Bing
B
Wu
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Qiulan
Q
Lv
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Mofeng
M
Ying
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China.
Xi
X
Zhang
Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People's Republic of China. liangsd@hotmail.com.
Shangdong
S
Liang
D000818:Animals; D015153:Blotting, Western; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000172:deficiency; D007511:Ischemia / Q000378:metabolism*; D010100:Oxygen; D016716:PC12 Cells; D016133:Polymerase Chain Reaction; D062085:RNA, Long Noncoding / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*
D062085:RNA, Long Noncoding; D058486:Receptors, Purinergic P2X7; C000611529:long non-coding RNA NONRATT021972, rat; D005947:Glucose; D010100:Oxygen
Adenosine triphosphate; Intracellular Ca2+; Long noncoding RNA; Oxygen-glucose deprivation; P2X7 receptor; PC12 cell