An In Vitro Evaluation of Biochemical Processes Involved in Lead-Induced Changes on Ram Spermatozoa.
Lead (Pb(2+) ) is a toxic heavy metal which interferes with several physiological processes regulated by Ca(2+) , including those characterized by changes of the membrane stability and the motility of spermatozoa necessary for the fertilization of the oocyte. In this study, ejaculated sperm from six rams (Ovis aries) have been incubated in vitro with or without 50 ng Pb(2+) /ml during 30 min and in the presence or absence of three different potential modulators of the effects of Pb(2+) on changes in the sperm membrane before fertilization: charybdotoxin, quinacrine and staurosporine. Sperm samples incubated with Pb(2+) have shown significant reductions in acrosome integrity and sperm viability and an increase in progressive movement. None of the studied potential modulators had a protective effect against Pb(2+) action. On the contrary, Pb(2+) -incubated sperm in the presence of staurosporine had lower acrosome integrity, and lower sperm viability was observed when spermatozoa were incubated with Pb(2+) + charybdotoxin. Quinacrine was the only tested substance capable of increasing the concentration of Pb(2+) in spermatozoa; thus, the enhancement of Pb(2+) effects produced by staurosporine and charybdotoxin was not produced by an increased uptake of Pb(2+) by spermatozoa. However, the increase of intracellular Pb(2+) in those spermatozoa incubated with quinacrine did not result in an adverse effect on sperm motility or viability although the acrosome integrity was negatively affected.
Instituto de Investigación en Recursos Cinegéticos (IREC) UCLM-CSIC-JCCM, Wildlife Toxicology Group, Ciudad Real, Spain.
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Castellanos
SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC) UCLM-CSIC-JCCM, Albacete, Spain.
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Maroto-Morales
SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC) UCLM-CSIC-JCCM, Albacete, Spain.
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García-Álvarez
SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC) UCLM-CSIC-JCCM, Albacete, Spain.
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Garde
Instituto de Investigación en Recursos Cinegéticos (IREC) UCLM-CSIC-JCCM, Wildlife Toxicology Group, Ciudad Real, Spain.
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Mateo
D000177:Acrosome / Q000187:drug effects; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D018999:Charybdotoxin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007854:Lead / Q000032:analysis / Q000494:pharmacology*; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011796:Quinacrine / Q000494:pharmacology; D034561:Sheep, Domestic; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D019311:Staurosporine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D008996:Monoamine Oxidase Inhibitors; D026902:Potassium Channel Blockers; D018999:Charybdotoxin; D007854:Lead; D011493:Protein Kinase C; D011796:Quinacrine; D019311:Staurosporine