The mitochondrial genome sequence of a deep-sea, hydrothermal vent limpet, Lepetodrilus nux, presents a novel vetigastropod gene arrangement.
While mitochondrial (mt) genomes are used extensively for comparative and evolutionary genomics, few mt genomes of deep-sea species, including hydrothermal vent species, have been determined. The Genus Lepetodrilus is a major deep-sea gastropod taxon that occurs in various deep-sea ecosystems. Using next-generation sequencing, we determined nearly the complete mitochondrial genome sequence of Lepetodrilus nux, which inhabits hydrothermal vents in the Okinawa Trough. The total length of the mitochondrial genome is 16,353bp, excluding the repeat region. It contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region, typical of most metazoan genomes. Compared with other vetigastropod mt genome sequences, L. nux employs a novel mt gene arrangement. Other novel arrangements have been identified in the vetigastropod, Fissurella volcano, and in Chrysomallon squamiferum, a neomphaline gastropod; however, all three gene arrangements are different, and Bayesian inference suggests that each lineage diverged independently. Our findings suggest that vetigastropod mt gene arrangements are more diverse than previously realized.
Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan. Electronic address: yuichi.nakajima@oist.jp.
Yuichi
Y
Nakajima
Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
Chuya
C
Shinzato
Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
Mariia
M
Khalturina
School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610, Japan.
Masako
M
Nakamura
Japan Agency for Marine-Earth Science and Technology, Yokosuka, Kanagawa 237-0061, Japan.
Hiromi
H
Watanabe
Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
Noriyuki
N
Satoh
Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.
Satoshi
S
Mitarai
D000818:Animals; D049851:Gastropoda / Q000235:genetics*; D023061:Gene Order; D054629:Genome, Mitochondrial; D060086:Hydrothermal Vents; D010140:Pacific Ocean; D010802:Phylogeny; D017422:Sequence Analysis, DNA
Mitogenome; Next-generation sequencing; Okinawa Trough; Vetigastropoda