Simultaneous determination of paeoniflorin from total glucosides of paeony in Sprague-Dawley rats and spontaneously hypertensive rats by high-performance liquid chromatography-tandem mass spectrometry: in vivo and in vitro studies.
Paeoniflorin is a well-known monoterpene glucoside in the herbal drug that exhibits a number of biological activities. The pharmacokinetic characteristics of paeoniflorin from total glucosides of paeony in spontaneously hypertensive rats (SHR) are still unclear. It is essential to investigate the in vivo and in vitro pharmacokinetic differences of paeoniflorin from total glucosides of paeony in Sprague-Dawley (SD) and SHR. The in vivo pharmacokinetic data were analyzed using DAS 2.0 software and the in vitro metabolic characteristics were measured using rat hepatic microsomes. The concentration of paeoniflorin in biological samples was determined using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method, which showed good precision and stability. The plasma concentration-time profiles of paeoniflorin following oral administration of total glucosides of paeony showed a single peak and there were significant differences in the mean values of AUC(0-t), AUC(0-∞), CLz/F and Tmaxbetween SD and SHR (p < 0.05). The metabolic rate of paeoniflorin from total glucosides of paeony was slower in SHR than in SD rats (p < 0.05). The results might be useful in further applications of paeoniflorin and total glucosides of paeony. Copyright © 2016 John Wiley & Sons, Ltd.
Institute of Materia Medica, Shandong Academy of Medical Sciences, Ji'nan, China. shanxiong83@sohu.com.
Shan
S
Xiong
School of Pharmacy, Yantai University, Yantai, China.
Yuyun
Y
Wang
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D005960:Glucosides / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D039821:Monoterpenes / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D029596:Paeonia / Q000737:chemistry; D011918:Rats, Inbred SHR; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods; D006027:Glycosides
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides
LC-MS/MS; Paeoniflorin; pharmacokinetics; spontaneously hypertensive rats; total glucosides of paeony