The algicidal activity of Aeromonas sp. strain GLY-2107 against bloom-forming Microcystis aeruginosa is regulated by N-acyl homoserine lactone-mediated quorum sensing.
Cyanobacterial blooms have disrupted the efficient utilization of freshwater worldwide. A new freshwater bacterial strain with strong algicidal activity, GLY-2107, was isolated from Lake Taihu and identified as Aeromonas sp. It produced two algicidal compounds: 2107-A (3-benzyl-piperazine-2,5-dione) and 2107-B (3-methylindole). Both compounds exhibited potent algicidal activities against Microcystis aeruginosa, the dominant bloom-forming cyanobacterium in Lake Taihu. The EC50values (concentration for 50% maximal effect) of 3-benzyl-piperazine-2,5-dione and 3-methylindole were 4.72 and 1.10 μg ml-1respectively. Based on a thin-layer chromatography biosensor assay and ultra-performance liquid chromatography-coupled high resolution-tandem mass spectrometry (UPLC-HRMS/MS), the N-acyl homoserine lactone (AHL) profile of strain GLY-2107 was identified as two short side-chain AHLs: N-butyryl-homoserine lactone (C4-HSL) and N-hexanoyl-homoserine lactone (C6-HSL). The production of the two algicidal compounds was controlled by AHL-mediated quorum sensing (QS), and C4-HSL was the key QS signal for the algicidal activity of the strain GLY-2107. Moreover, 3-methylindole was found to be positively regulated by C4-HSL-mediated QS, whereas 3-benzyl-piperazine-2,5-dione might be negatively controlled by C4-HSL-mediated QS. This study suggests that a QS-regulated algicidal system may have potential use for the development of a novel control strategy for harmful cyanobacterial blooms.
State Key Laboratory of Microbial metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China.
Xingliang
X
Guo
State Key Laboratory of Microbial metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China.
Xianglong
X
Liu
State Key Laboratory of Microbial metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China.
Lishuang
L
Wu
State Key Laboratory of Microbial metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China.
Jianliang
J
Pan
State Key Laboratory of Microbial metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P.R. China.
Hong
H
Yang
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D000333:Aeromonas / Q000235:genetics / Q000502:physiology*; D000898:Antibiosis; D002855:Chromatography, Thin Layer; D005068:Eutrophication; D060106:Lakes / Q000382:microbiology; D046931:Microcystis / Q000254:growth & development*; D053038:Quorum Sensing
C088386:homoserine lactone; D015107:4-Butyrolactone