Synthesis of a peptide-universal nucleotide antigen: towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes.
The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide , and four murine monoclonal antibodies raised against that exhibit excellent specificity for recognition of in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.
Department of Medicinal Chemistry, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA. daharki@umn.edu.
Angela L
AL
Perkins
Division of Oncology Research, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA.
Kevin L
KL
Peterson
Monoclonal Antibody Core, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA.
Thomas G
TG
Beito
Division of Oncology Research, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA.
Karen S
KS
Flatten
Division of Oncology Research, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA.
Scott H
SH
Kaufmann
Department of Medicinal Chemistry, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA. daharki@umn.edu.
Daniel A
DA
Harki
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D004264:DNA Topoisomerases, Type I / Q000737:chemistry* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D009711:Nucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D000941:Antigens; D009711:Nucleotides; D010455:Peptides; D004247:DNA; D004264:DNA Topoisomerases, Type I