Mur (MNS 10) screening with a novel loop-mediated isothermal amplification assay in Zhongshan, China.
BACKGROUND
The distribution of the Mur blood group antigen is 5-7% in the south of China, and a much higher prevalence is observed in some areas of the region. Anti-Mur can cause hemolytic disease of the newborn and severe transfusion reactions.


OBJECTIVE
Genetic testing is more ideal than conventional serological tests because antibodies for detection are usually not available.


METHODS
In this study, a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Mur blood group antigen was established.


RESULTS
Fifteen of 275 (5·5%) samples were confirmed by LAMP as Mur antigen positive. All the Mur antigen-positive samples were GP.Mur subtype which was confirmed with sequencing.


CONCLUSIONS
The LAMP method has identical results with conventional serology method but more suitable for large-scale screening.
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
S S
SS
Wei
Zhongshan Blood Center, Zhongshan, China.
A N
AN
Sun
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
S H
SH
Ding
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
Q L
QL
Meng
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
H M
HM
Wang
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
S B
SB
Duan
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
J J
JJ
Tian
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
Y Z
YZ
Chen
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
X M
XM
Liu
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
Y
Y
Li
Zhongshan Blood Center, Zhongshan, China.
Y T
YT
Liao
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005260:Female; D006801:Humans; D008951:MNSs Blood-Group System / Q000235:genetics*; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
D008951:MNSs Blood-Group System
GP. Mur; Miltenberger; loop-mediated isothermal amplification; molecular diagnosis