The in vitro cytotoxicity of metal-complexes of porphyrin sensitizer intended for photodynamic therapy.
The sulphonated derivatives of porphyrins (e.g. TPPS4) are hydrophilic photosensitizers and have certain advantages like fully known structures and the possibility of synthetic production. The aim of this work was to study in vitro cytotoxicity and to compare the new photosensitizer MgTPPS4 with TPPS4 and its other metal-complexes (ZnTPPS4, PdTPPS4) on human skin melanom and mouse fibroblast cell lines. A photodynamic treatment was induced by light emitting diodes with three different total doses (1, 5 and 10J/cm(2)). For proper analysis and understanding of cell behavior after the administration of sensitizers, a complex battery of in vitro tests including the production of reactive oxygen species, the MTT viability test, a comet assay, a cell cycle and a type of cell death determination were used. We discovered that the most suitable photosensitizer is ZnTPPS4 because it had the biggest lethal influence on melanoma cells and the lowest lethal influence on fibroblast cells. The second most effective photosensitizer seemed to be MgTPPS4. On the basis of our results we can also assume that there is a higher accumulation of photosensitizer in a tumorous cell line. The higher concentration of photosensitizer and light dose resulted in more reactive oxygen species production and found more cells undergoing necrosis.
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 5, 775 15 Olomouc, Czech Republic. Electronic address: LuckasMalina@seznam.cz.
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Malina
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 5, 775 15 Olomouc, Czech Republic. Electronic address: Katerina.Barton@upol.cz.
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Tomankova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 5, 775 15 Olomouc, Czech Republic.
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Malohlava
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 5, 775 15 Olomouc, Czech Republic.
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Jiravova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 5, 775 15 Olomouc, Czech Republic.
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Manisova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
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Zapletalova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
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Kolarova
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D020552:Comet Assay; D056831:Coordination Complexes / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008027:Light; D008665:Metalloporphyrins / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D010165:Palladium / Q000494:pharmacology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D056831:Coordination Complexes; D008665:Metalloporphyrins; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; C568228:magnesium 5,10,15,20-tetrakis(4-sulphophenyl)-porphine dodecahydrate; C474512:zinc-tetrakis(p-sulfonatophenyl)porphyrin; D010165:Palladium
Atomic force microscopy; Cell cycle; Genotoxicity; Photodynamic therapy; Porphyrin; Reactive oxygen species