[Establishment of migraine rheumatism stasis syndrome animal model].
OBJECTIVE
To establish the migraine rheumatism stasis syndrome animal model.


METHODS
The rat migraine rheumatism stasis syndrome animal model was established through rheumatism stimulation with manual climate box, 5-HT reduction caused by reserpine and local cerebral vasospasm. General vital signs (activity, weight, eye gum, hair, feeding, excrement), head scratch frequency and image collection were observed to analyze the changes in biological signs of stasis syndrome (tongue image RGB), thrombin and serotonin of model rats.


RESULTS
The reserpine group and the reserpine plus rheumatism model group showed significant reduction in blood coagulation time, pain threshold and 5-HT content in blood and brain (P < 0.01); the reserpine plus rheumatism model group showed an increase in eye gum and decreases in activity, feeding, with thin sloppy stool. According to the tough RGB values, the control group showed light red toughs, the reserpine group showed dark purple toughs, the reserpine plus rheumatism model group showed gray toughs, with notable differences in tough RGB values in all three group.


CONCLUSIONS
The rheumatism stimulation with manual climate box, 5-HT reduction caused by reserpine and local cerebral vasospasm can be used to induce the migraine rheumatism stasis syndrome animal model, but its modeling assessment method and process shall be further improved.
Hui
H
Chen
Gang
G
Luo
Xue
X
Bai
Si-jin
SJ
Yang
D000818:Animals; D001775:Blood Circulation; D003937:Diagnosis, Differential; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008881:Migraine Disorders / Q000175:diagnosis* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012216:Rheumatic Diseases / Q000175:diagnosis* / Q000503:physiopathology