Nanonets Collect Cancer Secretome from Pericellular Space.
Identifying novel cancer biomarkers is important for early cancer detection as it can reduce mortality rates. The cancer secretome, the collection of all macromolecules secreted by a tumor cell, alters its composition compared to normal tissue, and this change plays an important role in the observation of cancer progression. The collection and accurate analysis of cancer secretomes could lead to the discovery of novel biomarkers, thus improving outcomes of cancer treatment. We unexpectedly discovered that enzyme-instructed self-assembly (EISA) of a D-peptide hydrogelator results in nanonets/hydrogel around cancer cells that overexpress ectophosphatases. Here we show that these nanonets are able to rapidly collect proteins in the pericellular space (i.e., near the surface) of cancer cells. Because the secretory substances are at their highest concentration near the cell surface, the use of pericellular nanonets to collect the cancer secretome maximizes the yield and quality of samples, reduces pre-analytical variations, and allows the dynamic profiling of secretome samples. Thus, this new approach has great potential in identifying the heterotypic signaling in tumor microenvironments thereby improving the understanding of tumor microenvironments and accelerating the discovery of potential biomarkers in cancer biology. Data are available via ProteomeXchange with identifier PXD003924.
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Rong
0000-0002-3173-2322
R
Zhou
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Yi
Y
Kuang
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Jie
J
Zhou
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Xuewen
X
Du
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Jie
J
Li
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Junfeng
J
Shi
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Richard
R
Haburcak
Department of Chemistry, Brandeis University, Waltham, Massachusetts, United States of America.
Bing
B
Xu
D014408:Biomarkers, Tumor / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D013058:Mass Spectrometry; D049329:Nanostructures / Q000737:chemistry; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D055571:Secretory Pathway
D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D020543:Proteome; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate