Chromosome and genetic testing using ChIP assay.
Chromatin immunoprecipitation (ChIP) assay can be used to easily visualize information about proteins, DNA, and RNA on chromosomes and is widely used for analysis of genomes, epigenomes, mRNAs, and non-coding RNAs. The ChIP assay can detect, not only DNA-binding proteins of various organisms, but also the temporal and spatial regulating mechanisms of RNA-binding proteins. Because of these features, demand for ChIP assay is expected to grow. Here, by using yeast and Drosophila as examples, we describe the superiority of the improved ChIP assay that we have developed.
Faculty of Allied Health Science, Yamato University, Katayama-cho 2-52-3, Suita, Osaka 564-0082, Japan and Department of Cell Biology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto, Japan 606-8507, charaznable.k@gmail.com.
Hidetsugu
H
Kohzaki
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA and Department of Molecular Cellular and Biochemistry, The Ohio State Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
Maki
M
Asano
D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002875:Chromosomes / Q000737:chemistry* / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D012313:RNA / Q000032:analysis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D012313:RNA; D004247:DNA