Biochemical sensing by nanofluidic crystal in a confined space.
Electrokinetics at nanoscale has attracted broad attention as a promising conductivity based biochemical sensing principle with a good selectivity. The nanoparticle crystal, formed by self-assembling nanoparticles inside a microstructure, has been utilized to fulfill a nanoscale electrokinetics based biochemical sensing platform, named nanofluidic crystal in our previous works. This paper introduces a novel nanofluidic crystal scheme by packing nanoparticles inside a well-designed confined space to improve the device-to-device readout consistency. A pair of electrodes was patterned at the bottom of this tunnel-shaped confined space for ionic current recording. The readout from different chips (n = 16) varied within 8.4% under the same conditions, which guaranteed a self-calibration-free biochemical sensing. Biotin and Pb(2+) were successfully detected by using nanofluidic crystal devices packed with streptavidin and DNAzyme modified nanoparticles, respectively. The limits of detection (LODs) were both 1 nM. This electrically readable nanofluidic crystal sensing approach may find applications in low cost and fast disease screening in limited resource environments.
Institute of Microelectronics, Peking University, Beijing, 100871, China. w.wang@pku.edu.cn.
Wenda
W
Zhao
Institute of Microelectronics, Peking University, Beijing, 100871, China. w.wang@pku.edu.cn.
Baojun
B
Wang
Institute of Microelectronics, Peking University, Beijing, 100871, China. w.wang@pku.edu.cn and National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Beijing, 100871, China and Innovation Center for Micro-Nano-electronics and Integrated System, Beijing, 100871, China.
Wei
W
Wang
D001710:Biotin / Q000032:analysis; D004867:Equipment Design; D005898:Glass / Q000737:chemistry; D007854:Lead / Q000032:analysis; D057230:Limit of Detection; D008962:Models, Theoretical; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry; D014992:Xylenes / Q000737:chemistry
D011108:Polymers; D014992:Xylenes; C011055:parylene; D007854:Lead; D001710:Biotin