Comparison of Antioxidant Capability after Isopropanol Salting-Out Pretreatment and n-Butanol Partition Extraction, and Identification and Evaluation of Antioxidants of Sedum formosanum N.E.Br.
Crude extracts of Sedum formosanum N.E.Br. obtained from n-butanol partition (BP) and isopropanol salting-out pretreatment (ISP) were analyzed using antioxidation assays. The results indicated that the extract from ISP contained more potent antioxidants and thus exhibited more antioxidant activity in all the assays. The superoxide radical-scavenging activity and inhibition of nitric oxide radicals achieved after ISP were 3.65 and 2.18 times higher than those achieved through BP, respectively. Eight bioactive natural products were isolated and identified according to an analysis of antioxidation activity in different fractions of the ISP crude extract, namely three cyanophoric glycosides 1-3, three flavonoids 4-6 and two phenolic compounds (7 and a new compound 8). Among them, compounds 5 and 6 exhibit the highest antioxidation capability, and the ISP is suitable for obtaining compounds 5 and 6 using HPLC chromatograms. Therefore, ISP is an excellent extraction technology that can be used to extract antioxidant compounds in the nutraceutical and pharmaceutical industries.
Department of Mechanical Engineering, Nan-Kai University of Technology, 568 ZhongZheng Road, Caotun, Nantou County 542, Taiwan. t115@nkut.edu.tw.
Jung-Hui
JH
Chen
Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan. David751008@gmail.com.
Wen-Hui
WH
Lai
Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan. gloom2007@hotmail.com.
Shang-Dung
SD
Lin
Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan. mark921117@gmail.com.
Cheng-Fong
CF
Lan
Department of Medical Research, Taichung Veterans General Hospital, 1650 Taiwan Road Sec, Taichung 402, Taiwan. h2326@vghtc.gov.tw.
Shih-Lan
SL
Hsu
Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan. mliao@dragon.nchu.edu.tw.
Ming-Yuan
MY
Liao
D020001:1-Butanol / Q000737:chemistry*; D019840:2-Propanol / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004345:Drug Industry / Q000379:methods; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D031963:Sedum / Q000737:chemistry*
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D020001:1-Butanol; D019840:2-Propanol
S. formosanum N.E.Br.; antioxidant activity; cyanophoric glycoside; flavonoids; salting-out