Activity of 2,4-Di-tert-butylphenol produced by a strain of Streptomyces mutabilis isolated from a Saharan soil against Candida albicans and other pathogenic fungi.
In a search for new antifungal antibiotics active against Candida albicans and others pathogenic fungi, a strain of actinobacteria, designated G61, was isolated from a Saharan soil and tested for its activity against these microorganisms. The analysis of its 16S rDNA sequence showed a similarity level of 100% with Streptomyces mutabilis NBRC 12800(T). The highest anticandidal activities produced by the strain G61 were obtained on Bennett medium in the fourth day of incubation. The active product, extracted by n-butanol, contained one bioactive spot detected on thin layer chromatography plates. It was purified by HPLC and its chemical structure was determined by spectroscopic analyses as 2,4-Di-tert-butylphenol. The minimum inhibitory concentrations (MIC) of this product against several strains of pathogenic microorganisms are interesting.
Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria.
S
S
Belghit
Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria.
E H
EH
Driche
Laboratoire de chimie de coordination (LCC), CNRS, université de Toulouse, UPS, INPT, LCC, 205, 12, route de Narbonne, 31077 Toulouse, France.
C
C
Bijani
Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria.
A
A
Zitouni
Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria.
N
N
Sabaou
Laboratoire de Biologie des Systèmes Microbiens (LBSM), École Normale Supérieure de Kouba, Alger, Algeria. Electronic address: badji_boubekeur@yahoo.fr.
B
B
Badji
Laboratoire de Génie Chimique, LGC, Université de Toulouse, UMR 5503 (CNRS/INPT/UPS), Toulouse, France.
F
F
Mathieu
D000352:Africa, Northern; D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D000935:Antifungal Agents; D010636:Phenols; D012336:RNA, Ribosomal, 16S; C056559:2,4-di-tert-butylphenol
2,4-Di-tert-butylphenol; 2,4-Di-tert-butylphénol; Activité antifongique; Antifungal activity; Candida albicans; Streptomyces; Taxonomie; Taxonomy