Preparation and partial structural characterization of the exopolysaccharide from Bacillus mucilaginosus SM-01.
In this study, an acidic hetero-exopolysaccharide, designated as BMPS, was isolated from the culture supernatant of bacteria Bacillus mucilaginosus SM-01 by ion-exchange and size-exclusion chromatography. The molecular weight of BMPS was measured to be 2.67×10(6)Da. To analyze the structure of the polysaccharide, the low-molecular weight fragment (BMPS-H) of BMPS was obtained under mild hydrolysis. BMPS-H was determined to be a liner neutral fraction with an average molecular weight of 1621Da. Monosaccharide analysis indicated that BMPS-H contained glucose and mannose in a molar ratio of 1.5:1. Based on methylation analysis and NMR spectroscopy data, the following structure of BMPS-H was established: In addition, MALDI-TOF-MS analysis showed the hydroxyl group on C-2 of 1,4-linked-glucosyl residues was partially acetylated.
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China. Electronic address: yuli0055@163.com.
Li
L
Yu
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: shuqinxu_2015@163.com.
Shuqin
S
Xu
Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China. Electronic address: deng_c@sina.com.
Chao
C
Deng
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: lihui@jiangnan.edu.cn.
Hui
H
Li
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: qingsheng.yang@163.com.
Qingsheng
Q
Yang
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: zhenghxu@jiangnan.edu.cn.
Zhenghong
Z
Xu
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: jhchenwhut@126.com.
Jinghua
J
Chen
D001407:Bacillus / Q000737:chemistry*; D002850:Chromatography, Gel; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial
Bacillus mucilaginosus; Exopolysaccharide; Methylation analysis; Nuclear magnetic resonance (NMR) spectroscopy; Structure