Identification of the microRNA networks contributing to macrophage differentiation and function.
Limited evidence is available about the specific miRNA networks that regulate differentiation of specific immune cells. In this study, we characterized miRNA expression and associated alterations in expression with putative mRNA targets that are critical during differentiation of macrophages. In an effort to map the dynamic changes in the bone marrow (BM), we profiled whole BM cultures during differentiation into macrophages. We identified 112 miRNAs with expression patterns that were differentially regulated 5-fold or more during BMDM development. With TargetScan and MeSH databases, we identified 1267 transcripts involved in 30 canonical pathways linked to macrophage biology as potentially regulated by these specific 112 miRNAs. Furthermore, by employing miRanda and Ingenuity Pathways Analysis (IPA) analysis systems, we identified 18 miRNAs that are temporally linked to the expression of CSF1R, CD36, MSR1 and SCARB1; 7 miRNAs linked to the regulation of the transcription factors RUNX1 and PU.1, and 14 miRNAs target the nuclear receptor PPARα and PPARγ. This novel information provides an important reference resource for further study of the functional links between miRNAs and their target mRNAs for the regulation of differentiation and function of macrophages.
Department of Respiratory Medicine, The Second Hospital, Jilin University, ChangChun, Jilin, People's Republic of China.|Priority Research Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, The University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW, Australia.
Hong
H
Zhou
Department of Respiratory Medicine, The Second Hospital, Jilin University, ChangChun, Jilin, People's Republic of China.
Jie
J
Zhang
Priority Research Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, The University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW, Australia.
Fiona
F
Eyers
Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China.
Yang
Y
Xiang
Inflammation and Infection Research Centre, School of Medical Sciences, Sydney, UNSW, Australia.
Cristan
C
Herbert
Priority Research Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, The University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW, Australia.
Hock L
HL
Tay
Priority Research Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, The University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW, Australia.
Paul S
PS
Foster
Priority Research Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, The University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW, Australia.
Ming
M
Yang
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D053263:Gene Regulatory Networks / Q000235:genetics*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*
D035683:MicroRNAs
Immune response; Immunity; Immunology and Microbiology Section; differentiation; macrophage; microRNA; transcriptional regulation