UBIQUITIN-SPECIFIC PROTEASE14 Interacts with ULTRAVIOLET-B INSENSITIVE4 to Regulate Endoreduplication and Cell and Organ Growth in Arabidopsis.
Organ growth is determined by a coordinated combination of cell proliferation and cell growth and differentiation. Endoreduplication is often coupled with cell growth and differentiation, but the genetic and molecular mechanisms that link endoreduplication with cell and organ growth are largely unknown. Here, we describe UBIQUITIN-SPECIFIC PROTEASE14 (UBP14), encoded by the DA3 gene, which functions as a negative regulator of endoreduplication. The Arabidopsis thaliana da3-1 mutant shows large cotyledons, leaves, and flowers with higher ploidy levels. UBP14 acts along with UV-B-INSENSITIVE4 (UVI4), an inhibitor of the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase, to repress endoreduplication. Also, UBP14 functions antagonistically with CELL CYCLE SWITCH52 A1 (CCS52A1), an activator of APC/C, to regulate endoreduplication. UBP14 physically associates with UVI4 both in vitro and in vivo but does not directly interact with CCS52A1. Further results reveal that UBP14 influences the stability of cyclin A2;3 (CYCA2;3) and cyclin-dependent kinase B1;1 (CDKB1;1), two downstream components of the APC/C Thus, our findings show how endoreduplication is linked with cell and organ growth by revealing important genetic and molecular functions for the ubiquitin-specific protease UBP14 and for the key cell cycle regulators UVI4, CCS52A1, CYCA2;3, and CDKB1;1.
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yingxiu
Y
Xu
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Weihuan
W
Jin
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Na
N
Li
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Wenjuan
W
Zhang
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Cuimin
0000-0003-4982-4804
C
Liu
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China yhli@genetics.ac.cn cyli@genetics.ac.cn.
Chuanyou
0000-0003-0202-3890
C
Li
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China yhli@genetics.ac.cn cyli@genetics.ac.cn.
Yunhai
0000-0003-0025-4444
Y
Li
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D011485:Protein Binding; D064570:Ubiquitin-Specific Proteases / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C540491:FZR2 protein, Arabidopsis; C491560:UBP14 protein, Arabidopsis; C434472:CDKA1 protein, Arabidopsis; D018844:Cyclin-Dependent Kinases; D010450:Endopeptidases; D064570:Ubiquitin-Specific Proteases