Relationship between XspI Site Polymorphisms of LDL-R Gene and Serum IL-2 and IL-10 in Patients with Hypercholesterolemia.
BACKGROUND
Relationship has been identified in sporadic reports between polymorphisms and hypercholesterolemia. However, the relationship between inflammatory cytokines and polymorphism of low-density lipoprotein receptor (LDL-R) gene in hypercholesterolemia is unclear. This study aimed to explore the relationship and significance between polymorphisms of LDL-R gene and serum Interleukin-2 (IL-2), IL-10 in patients with hypercholesterolemia.


METHODS
PCR-RFLP and direct DNA sequencing assay were employed to determine polymorphism of LDL-R gene in 900 patients with hypercholesterolemia and 400 healthy cases. ELISA was applied to assay serum concentration of IL-2 and IL-10. Blood lipid indexes were tested in all cases.


RESULTS
Compared with the healthy controls, level of IL-2 increased significantly, while IL-10 decreased significantly (P < 0.05). Correlation analysis showed that IL-2 was positively correlated with total cholesterol (TC), LDL-c, and genotype (r = 0.542, 0.410, 0.598, P < 0.05) and negatively correlated with HDL-c (r = -0.352, P < 0.05). Negative relationship also was found between TC, LDL-c, genotype, and IL-10 (r = -0.452, -0.390, -0.613, P < 0.05), and positive correlation between HDL-c and IL-10 (r = 0.398, P < 0.05). Multiple linear regression showed that genotypes and TC were independent factors affecting the levels of IL-2 and IL-10 (P < 0.05).


CONCLUSIONS
IL-2 and IL-10 were related to gene polymorphisms of LDL-R, which might be involved in the development and progress of hypercholesterolemia.
Clinical laboratory, Hebei General Hospital, Shijiazhuang, China. zhangmm197612@126.com.
Mingming
M
Zhang
Department of nuclear medicine, Hebei General Hospital, Shijiazhuang, China.
Yamin
Y
Lu
Function Department, Hebei General Hospital, Shijiazhuang, China.
Xin
X
Liu
Clinical laboratory, Second Hospital of Hebei Medical University, Shijiazhuang, China.
Xiaobin
X
Zhang
Physical examination center, Hebei General Hospital, Shijiazhuang, China.
Cuigai
C
Zhang
Clinical laboratory, Hebei General Hospital, Shijiazhuang, China.
Wei
W
Gao
Clinical laboratory, Hebei General Hospital, Shijiazhuang, China.
Yanqing
Y
Tie
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D004252:DNA Mutational Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood* / Q000235:genetics*; D016753:Interleukin-10 / Q000097:blood*; D007376:Interleukin-2 / Q000097:blood*; D016014:Linear Models; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011973:Receptors, LDL / Q000235:genetics*
D007376:Interleukin-2; D008055:Lipids; D011973:Receptors, LDL; D016753:Interleukin-10
gene polymorphisms; hypercholesterolemia; interleukin-10; interleukin-2; low-density lipoprotein receptor