[Effect of adaptation to hypoxia on expression of NO synthase isoforms in rat myocardium].
Previously we have shown that adaptation to hypoxia (AH) is cardio- and vasoprotective in myocardial ischemic and reperfusion injury and this protection is associated with restriction of nitrosative stress. The present study was focused on further elucidation of NO-dependent mechanisms of AH by identifying specific NO synthases (NOS) that could play the major role in AH protection. AH was performed in a normobaric hypoxic chamber by breathing hypoxic gas mixture (9.5-10% O2) for 5-10 min with intervening 4 min normoxia (5-8 cycles daily for 21 days). Expression of neuronal (nNOS), inducible (iNOS), and endothelial (eNOS) protein was measured in the left ventricular myocardium using Western blot analysis with respective antibodies. AH educed iNOS protein expression by 71% (p < 0.05) whereas eNOS protein expression tended to be reduced by 41% compared to control (p < 0.05). nNOS protein expression remained unchanged after AH. Selective iNOS inhibition can mimic the AH-induced protection. Therefore protective effects of AH could be at least partially due to restriction of iNOS and, probably, eNOS expression.
A V
AV
Goryacheva
O L
OL
Terekhina
D V
DV
Abramochkin
O P
OP
Budanova
L M
LM
Belkina
B V
BV
Smirin
H F
HF
Downey
I Yu
IY
Malyshev
E B
EB
Manukhina
D000222:Adaptation, Physiological; D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D000860:Hypoxia / Q000201:enzymology*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D052248:Nitric Oxide Synthase Type I / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis*; D051381:Rats
D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496322:Nos1 protein, rat; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat