HPLC enantioseparation of racemic bupropion, baclofen and etodolac: modification of conventional ligand exchange approach by pre-column formation of chiral ligand exchange complexes.
Separation of racemic mixture of (RS)-bupropion, (RS)-baclofen and (RS)-etodolac, commonly marketed racemic drugs, has been achieved by modifying the conventional ligand exchange approach. The Cu(II) complexes were first prepared with a few l-amino acids, namely, l-proline, l-histidine, l-phenylalanine and l-tryptophan, and to these was introduced a mixture of the enantiomer pair of (RS)-bupropion, or (RS)-baclofen or (RS)-etodolac. As a result, formation of a pair of diastereomeric complexes occurred by 'chiral ligand exchange' via the competition between the chelating l-amino acid and each of the two enantiomers from a given pair. The diastereomeric mixture formed in the pre-column process was loaded onto HPLC column. Thus, both the phases during chromatographic separation process were achiral (i.e. neither the stationary phase had any chiral structural feature of its own nor did the mobile phase have any chiral additive). Separation of diastereomers was successful using a C18column and a binary mixture of MeCN and TEAP buffer of pH 4.0 (60:40, v/v) as mobile phase at a flow rate of 1 mL/min and UV detection at 230 nm for (RS)-Bup, 220 nm for (RS)-Bac and 223 nm for (RS)-Etd. Baseline separation of the two enantiomers was obtained with a resolution of 6.63 in <15 min. Copyright © 2016 John Wiley & Sons, Ltd.
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India.
Manisha
M
Singh
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India. rbushfcy@iitr.ernet.in.
Ravi
R
Bhushan
D000596:Amino Acids / Q000737:chemistry; D018687:Antidepressive Agents, Second-Generation / Q000737:chemistry / Q000302:isolation & purification*; D001418:Baclofen / Q000737:chemistry / Q000302:isolation & purification*; D016642:Bupropion / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056831:Coordination Complexes / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D052246:Cyclooxygenase 2 Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D017308:Etodolac / Q000737:chemistry / Q000302:isolation & purification*; D008024:Ligands; D009125:Muscle Relaxants, Central / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
D000596:Amino Acids; D018687:Antidepressive Agents, Second-Generation; D056831:Coordination Complexes; D052246:Cyclooxygenase 2 Inhibitors; D008024:Ligands; D009125:Muscle Relaxants, Central; D016642:Bupropion; D017308:Etodolac; D003300:Copper; D001418:Baclofen
(RS)-baclofen; (RS)-bupropion; (RS)-etodolac; enantioresolution; l-amino acids; modified ligand exchange HPLC