Embryonic common snapping turtles (Chelydra serpentina) preferentially regulate intracellular tissue pH during acid-base challenges.
The nests of embryonic turtles naturally experience elevated CO2 (hypercarbia), which leads to increased blood PCO2  and a respiratory acidosis, resulting in reduced blood pH [extracellular pH (pHe)]. Some fishes preferentially regulate tissue pH [intracellular pH (pHi)] against changes in pHe; this has been proposed to be associated with exceptional CO2 tolerance and has never been identified in amniotes. As embryonic turtles may be CO2 tolerant based on nesting strategy, we hypothesized that they preferentially regulate pHi, conferring tolerance to severe acute acid-base challenges. This hypothesis was tested by investigating pH regulation in common snapping turtles (Chelydra serpentina) reared in normoxia then exposed to hypercarbia (13 kPa PCO2 ) for 1 h at three developmental ages: 70% and 90% of incubation, and yearlings. Hypercarbia reduced pHe but not pHi, at all developmental ages. At 70% of incubation, pHe was depressed by 0.324 pH units while pHi of brain, white muscle and lung increased; heart, liver and kidney pHi remained unchanged. At 90% of incubation, pHe was depressed by 0.352 pH units but heart pHi increased with no change in pHi of other tissues. Yearlings exhibited a pHe reduction of 0.235 pH units but had no changes in pHi of any tissues. The results indicate common snapping turtles preferentially regulate pHi during development, but the degree of response is reduced throughout development. This is the first time preferential pHi regulation has been identified in an amniote. These findings may provide insight into the evolution of acid-base homeostasis during development of amniotes, and vertebrates in general.
Department of Zoology, University of British Columbia, Vancouver, BC, Canada V6T 1Z4 shartau@zoology.ubc.ca.
Ryan B
0000-0002-0455-8496
RB
Shartau
Department of Biological Sciences, University of North Texas, Denton, TX 76203-5017, USA.
Dane A
DA
Crossley
Department of Biological Sciences, University of North Texas, Denton, TX 76203-5017, USA.
Zachary F
ZF
Kohl
Department of Zoology, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.
Colin J
CJ
Brauner
D000136:Acid-Base Equilibrium; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000502:physiology; D014018:Tissue Distribution; D014426:Turtles / Q000254:growth & development / Q000502:physiology*
D002245:Carbon Dioxide
Acid–base regulation; Amniotes; CO2; Development; Hypercarbia; Physiology; Preferential pHi regulation; Reptile