Well-Defined Peapod-like Magnetic Nanoparticles and Their Controlled Modification for Effective Imaging Guided Gene Therapy.
Due to their unique properties, one-dimensional (1D) magnetic nanostructures are of great significance for biorelated applications. A facile and straightforward strategy to fabricate 1D magnetic structure with special shapes is highly desirable. In this work, well-defined peapod-like 1D magnetic nanoparticles (Fe3O4@SiO2, p-FS) are readily synthesized by a facile method without assistance of any templates, magnetic string or magnetic field. There are few reports on 1D gene carriers based on Fe3O4 nanoparticles. BUCT-PGEA (ethanolamine-functionalized poly(glycidyl methacrylate) is subsequently grafted from the surface of p-FS nanoparticles by atom transfer radical polymerization to construct highly efficient gene vectors (p-FS-PGEA) for effective biomedical applications. Peapod-like p-FS nanoparticles were proven to largely improve gene transfection performance compared with ordinary spherical Fe3O4@SiO2 nanoparticles (s-FS). External magnetic field was also utilized to further enhance the transfection efficiency. Moreover, the as-prepared p-FS-PGEA gene carriers could combine the magnetic characteristics of p-FS to well achieve noninvasive magnetic resonance imaging (MRI). We show here novel and multifunctional magnetic nanostructures fabricated for biomedical applications that realized efficient gene delivery and real-time imaging at the same time.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.|Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology , Ministry of Education, Beijing 100029, China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029, China.
Ranran
R
Wang
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.|Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology , Ministry of Education, Beijing 100029, China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029, China.
Yang
Y
Hu
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.|Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology , Ministry of Education, Beijing 100029, China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029, China.
Nana
N
Zhao
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.|Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology , Ministry of Education, Beijing 100029, China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029, China.
Fu-Jian
FJ
Xu
D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D058105:Polymerization; D012822:Silicon Dioxide; D014162:Transfection
D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide
MRI imaging; gene transfection; magnetic nanoparticles; peapod-like; polycation