Simultaneous Production of Formylated and Nonformylated Enterocins L50A and L50B as well as 61A, a New Glycosylated Durancin, by Enterococcus durans 61A, a Strain Isolated from Artisanal Fermented Milk in Tunisia.
Enterococcus durans 61A, a broad-spectrum strain, was isolated from artisanal fermented dairy products. The strain is a multibacteriocin producer, free from virulence genes, and could be considered a good candidate for application in food preservation. In the present study, E. durans 61A was shown to produce simultaneously formylated and nonformylated forms of leaderless enterocins L50A and L50B as well as 61A, a new glycosylated durancin. Bacteriocins were characterized using mass spectrometry. Formylation was found to increase enterocin antimicrobial activity of enterocin L50A (8×) and, to a lesser extent, the activity of L50B (2×). Durancin 61A was found glycosylated by two hexoses (glucose and arabinose) and exhibited broad-spectrum inhibition against Gram-positive and Gram-negative bacteria and fungal spores. Durancin 61A was highly bactericidal at 15.6 μg/mL (10× the MIC) on Listeria innocua HPB13 and seems to target bacterial membrane as shown by ion efflux and transmission electron microscopy.
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval , Québec, QC, Canada G1K 7P4.|Unité de Protéomie Fonctionnelle & Biopréservation Alimentaire, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El Manar , Tunis, Tunisia.
Hasna
H
Hanchi
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval , Québec, QC, Canada G1K 7P4.
Riadh
R
Hammami
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval , Québec, QC, Canada G1K 7P4.
Benoit
B
Fernandez
Unité de Protéomie Fonctionnelle & Biopréservation Alimentaire, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El Manar , Tunis, Tunisia.
Rim
R
Kourda
Unité de Protéomie Fonctionnelle & Biopréservation Alimentaire, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université Tunis El Manar , Tunis, Tunisia.
Jeannette
J
Ben Hamida
STELA Dairy Research Centre, Institute of Nutrition and Functional Foods, Université Laval , Québec, QC, Canada G1K 7P4.
Ismail
I
Fliss
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D043302:Cultured Milk Products / Q000382:microbiology*; D016983:Enterococcus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D006031:Glycosylation; D015394:Molecular Structure; D014416:Tunisia
D000890:Anti-Infective Agents; D001952:Bridged-Ring Compounds; C012306:enterocin
Enterococcus durans 61A; durancin 61A; enterocin L50A; enterocin L50B; glycosylated bacteriocin