Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3.
A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China. chen-yx@mail.tsinghua.edu.cn.
Lei
L
Li
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China. chen-yx@mail.tsinghua.edu.cn.
Si-Yu
SY
Zhang
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China. chen-yx@mail.tsinghua.edu.cn.
Yan-Mei
YM
Li
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China. chen-yx@mail.tsinghua.edu.cn.
Yong-Xiang
YX
Chen
D015374:Biosensing Techniques; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D025841:Small Ubiquitin-Related Modifier Proteins / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling; D025801:Ubiquitin / Q000737:chemistry* / Q000378:metabolism; D043222:Ubiquitin Thiolesterase; D054875:Ubiquitination
D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin; C509806:UCHL3 protein, human; D043222:Ubiquitin Thiolesterase; D003546:Cysteine Endopeptidases