Highly efficient energy transfer from quantum dot to allophycocyanin in hybrid structures.
Excitation energy transfer (EET) is observed in hybrid structures that composed of allophycocyanin and CdSe/ZnS core-shell quantum dot (QD). We demonstrate that the EET efficiency in such systems could be significantly increased under conditions inducing monomerization of allophycocyanin trimers. For these purposes, the EET efficiency was estimated under different experimental conditions (pH, high temperature or the presence of NaSCN) for self-assembled hybrid structures. Additionally, the hybrid structures were stabilized by covalent coupling which resulted in approximately 20-fold enhancement of allophycocyanin fluorescence upon excitation of QDs. The observed effect provides new opportunities for the practical implementation of hybrid systems as fluorescent markers.
Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia; Institute of Physical Chemistry, Hamburg University, 20146 Hamburg, Germany. Electronic address: anastasi-kar@yandex.ru.
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Karpulevich
Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
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Maksimov
A.N.Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
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Sluchanko
Department of Low-Temperature Physics and Superconductivity, Faculty of Physics, Lomonosov Moscow State University, 119991 Moscow, Russia; Theoretical Physics and Applied Mathematics Department, Ural Federal University, 620002 Ekaterinburg, Russia.
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Vasiliev
Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
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Paschenko
D004735:Energy Transfer; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010798:Phycocyanin / Q000737:chemistry*; D045663:Quantum Dots; D013696:Temperature
C002497:allophycocyanin; D010798:Phycocyanin
Allophycocyanin; Excitation energy transfer; Fluorescence; Hybrid structures; Quantum dots