Hepcidin, Cathelicidin-1 and IL-8 as immunological markers of responsiveness in early developmental stages of rainbow trout.
During the early developmental stage of salmonids, high mortality occurs largely as a result of pathogens. These cause low immune competence in fry, producing disease, decreasing production and finally leading to economic losses. Therefore, the aim of this study was to characterise the developmental stages in which rainbow trout acquires immune response capability when challenged with LPS from Pseudomona aeruginosa for 8 h, studying the hepcidin, cathelicidin-1 and IL-8. Total RNA was extracted from fry at 34, 42, 56 and 66 days post hatching (dph). Hepcidin and cathelicidin-1 transcripts were detected only at days 34 and 42, whereas the IL-8 transcript was detected from day 34 to day 66. To analyse the protein expression in the fry, polyclonal anti-peptide antibodies were generated in rabbit. These three immune sera demonstrated the ability to recognise the whole molecule in biological samples. Immunofluorescence showed that skin, gills and intestine mainly responded to the LPS challenge, indicating that these portals of pathogen entry are capturing LPS. This study constitutes a valuable approach, since it has the potential to identify molecules with biological activity that can be used to evaluate the status of fry in culture.
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; Programa de Doctorado en Biotecnología Pontificia Universidad Católica de Valparaíso/Universidad Técnica Federico Santa María, Valparaíso, Chile; Núcleo Biotecnológico de Curauma (NBC), Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile. Electronic address: paula.santana@ucv.cl.
Paula A
PA
Santana
Núcleo Biotecnológico de Curauma (NBC), Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile. Electronic address: fanny.guzman@ucv.cl.
Fanny
F
Guzmán
Programa de Doctorado en Biotecnología Pontificia Universidad Católica de Valparaíso/Universidad Técnica Federico Santa María, Valparaíso, Chile. Electronic address: jcarlosforero@gmail.com.
Juan C
JC
Forero
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; Núcleo Biotecnológico de Curauma (NBC), Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile. Electronic address: omar.luna.g@gmail.com.
Omar F
OF
Luna
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile. Electronic address: luis.mercado@pucv.cl.
Luis
L
Mercado
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D005393:Fish Diseases / Q000276:immunology; D029941:Fish Proteins / Q000276:immunology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D064451:Hepcidins / Q000276:immunology / Q000378:metabolism*; D007113:Immunity, Innate; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D017686:Oncorhynchus mykiss / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D054804:Cathelicidins
D000906:Antibodies; D023181:Antimicrobial Cationic Peptides; D015415:Biomarkers; D029941:Fish Proteins; D064451:Hepcidins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D054804:Cathelicidins
Antimicrobial peptides; Fish innate immunity; LPS-challenge; Trout fry