Single nucleotide polymorphism analysis reveals heterogeneity within a seedling tree population of a polyembryonic mango cultivar.
Within a polyembryonic mango seedling tree population, the genetic background of individuals should be identical because vigorous plants for cultivation are expected to develop from nucellar embryos representing maternal clones. Due to the fact that the mango cultivar 'Hôi' is assigned to the polyembryonic ecotype, an intra-cultivar variability of ethylene receptor genes was unexpected. Ethylene receptors in plants are conserved, but the number of receptors or receptor isoforms is variable regarding different plant species. However, it is shown here that the ethylene receptor MiETR1 is present in various isoforms within the mango cultivar 'Hôi'. The investigation of single nucleotide polymorphisms revealed that different MiETR1 isoforms can not be discriminated simply by individual single nucleotide exchanges but by the specific arrangement of single nucleotide polymorphisms at certain positions in the exons of MiETR1. Furthermore, an MiETR1 isoform devoid of introns in the genomic sequence was identified. The investigation demonstrates some limitations of high resolution melting and ScreenClust analysis and points out the necessity of sequencing to identify individual isoforms and to determine the variability within the tree population.
University of Hohenheim, Institute of Crop Science, Crop Physiology of Specialty Crops, Stuttgart, Germany.|University of Hohenheim, Institute of Crop Science, Crop Physiology of Specialty Crops, Stuttgart, Germany.
Patrick
P
Winterhagen
University of Hohenheim, Institute of Crop Science, Crop Physiology of Specialty Crops, Stuttgart, Germany.|University of Hohenheim, Institute of Crop Science, Crop Physiology of Specialty Crops, Stuttgart, Germany.
Jens-Norbert
JN
Wünsche
D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D018740:Genetic Heterogeneity; D031022:Mangifera / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms; D011956:Receptors, Cell Surface / Q000235:genetics; D036226:Seedlings / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014197:Trees / Q000235:genetics*
D010940:Plant Proteins; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant
Mangifera indica; ScreenClust; dénaturation à haute résolution; ethylene receptor ETR1; high resolution melting; intra-cultivar sequence variability; récepteur de l’éthylène ETR1; variation nucléotidique intra-variétale