[Effect of di-(2-ethylhexyl) phthalate exposure on placental development in pregnant mice].
OBJECTIVE
To investigate the effect of di-(2-ethylhexyl) phthalate (DEHP) exposure on the growth and development of placenta, uterine natural killer (uNK) cell number and angiogenesis at the maternal-fetal interface in pregnant mice.


METHODS
From day 1 of pregnancy, pregnant mice were exposed daily to DEHP by oral gavage at 125, 250, or 500 mg/kg for 13 consecutive days. The uterine and placental tissues were then harvested for HE staining and immunohistochemistry to examine the effect of DEHP exposure on the growth and development of the placenta and angiogenesis and uNK cell number at the maternal-fetal interface.


RESULTS
Compared with the control group, the mice exposed to 500 mg/kg DEHP, but not those exposed to 125 and 250 mg/kg, showed significantly reduced number of embryo implantation (P<0.05). DEHP exposure significantly increased the rate of abortion. DEHP exposure at 125, 250, and 500 mg/kg significantly and dose-dependently lowered the placental weight compared with that in the control group (0.0637±0.0133, 0.0587±0.0176, 0.0524±0.0183 g vs 0.0786±0.0143 g, respectively; P<0.01), and significantly reduced the total area of the placenta and area of spongiotrophoblasts. DEHP exposure resulted in a significant reduction in the number of fetal vascular branches, and collapse and atresia of blood vessels. The mice exposed to DEHP at 125, 250, and 500 mg/kg had significantly lowered numbers of uNK cells (83.2±10.3, 60.7±12.4, and 50.4±14.5/HP, respectively) as compared with the control group (105.1±14.2/HP) at the maternal-fetal interface (P<0.01).


CONCLUSIONS
DEHP exposure significantly affects the growth and development of the placenta in mice possibly by suppressing angiogenesis and reducing uNK cell number at the maternal-fetal interface during pregnancy.
Department of Physiology, School of Medicine, Nanchang University, Nanchang 330006, China. E-mail: 13393733537@163.com.
Lu
L
Zhang
Teng-Ling
TL
Zhang
Teng
T
Zong
Yi-Lu
YL
Chen
Min
M
Ren
Xiao-Chun
XC
Yu
Hai-Bin
HB
Kuang
D000818:Animals; D004051:Diethylhexyl Phthalate / Q000009:adverse effects*; D010064:Embryo Implantation; D005260:Female; D005312:Fetal Blood; D007694:Killer Cells, Natural / Q000166:cytology; D018811:Maternal Exposure / Q000009:adverse effects*; D051379:Mice; D018919:Neovascularization, Physiologic; D010920:Placenta / Q000187:drug effects*; D010929:Placentation / Q000187:drug effects*; D011247:Pregnancy; D014599:Uterus / Q000187:drug effects*
D004051:Diethylhexyl Phthalate