Long noncoding RNA NEAT1 is an unfavorable prognostic factor and regulates migration and invasion in gastric cancer.
BACKGROUND
Long noncoding RNAs (LncRNAs) have been demonstrated as playing important roles in diverse biological processes including tumorigenesis. However, the clinical significance and biological function of LncRNA nuclear-enriched abundant transcript 1 (NEAT1) in gastric cancer are still unknown. The aim of this study was to identify the role of LncRNA NEAT1 in gastric cancer.


METHODS
The expression of LncRNA NEAT1 was detected in gastric cancer samples and cell lines by real-time PCR. The clinical and prognostic significance of LncRNA NEAT1 in gastric cancer patients was analyzed. Furthermore, the biological function of LncRNA NEAT1 on tumor cell growth and mobility were explored through MTT, colony formation, transwell migration, and invasion assays in vitro. The potential mechanism of LncRNA NEAT1 was identified by Western blot.


RESULTS
LncRNA NEAT1 was overexpressed in gastric cancer tissues and cell lines and corrected with clinical stage, histological type, lymph node metastasis, and distant metastasis. Furthermore, patients with high levels of LncRNA NEAT1 had poorer survival than those with lower levels of LncRNA NEAT1. Univariate and multivariate Cox regression analyses showed that LncRNA NEAT1 overexpression was a poor independent prognostic factor for gastric cancer patients. Moreover, knocking down LncRNA NEAT1 expression significantly suppressed the gastric cancer cell migration and invasion in vitro and regulated EMT-associated proteins expression.


CONCLUSIONS
LncRNA NEAT1 plays an important role on gastric cancer tumorigenesis and progression and may act as a potential biomarker for therapeutic strategy and prognostic prediction.
Department of Gastrointestinal Surgery, Jining No. 1 People's Hospital, No. 6, Jiankang Road, Jining City, 272011, Shandong Province, People's Republic of China.
Jing-Wei
JW
Fu
Department of Gastrointestinal Surgery, Jining No. 1 People's Hospital, No. 6, Jiankang Road, Jining City, 272011, Shandong Province, People's Republic of China.
Ying
Y
Kong
Department of Gastrointestinal Surgery, Shandong Cancer Hospital and Institute, No. 440, Jiyan Road, Jinan City, 250117, Shandong Province, People's Republic of China. sunxuzmz@126.com.
Xu
X
Sun
D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
EMT; Gastric cancer; LncRNA; MMP; Metastasis; NEAT1