Degradation of dentin-bonded interfaces treated with collagen cross-linking agents in a cariogenic oral environment: An in situ study.
To evaluate the effect of treatment using collagen cross-linking agents as primer on resin-dentin bond interfaces subjected to cariogenic oral environment (COE).



Each of forty human teeth had two cavities (4×4×1.5mm) prepared within enamel margins. These cavities were acid-etched and treated by the primers containing one of the following treatment agents (6.5% proanthocyanidins, 0.1% riboflavin-UVA activated light, 5% glutaraldehyde or distilled water as a control group). After that the cavities were bonded and restored with resin composite. One restoration for each tooth was tested immediately (IM) and another was included in an intra-oral palatal device that was placed in each mouth of ten adult volunteers for 14 days in COE. After 14 days, the teeth were removed and each restoration was sectioned to obtain a slice for Knoop microhardness (KHN) and resin-dentin bonded sticks for microtensile bond strength (μTBS) and nanoleakage (NL) evaluation. Data were evaluated by two-way ANOVA and Tukey's tests (α=0.05).



After 14days in a COE, the KHN was reduced for all groups, except for the glutaraldehyde group; however, the proanthocyanidins group retained the highest KHN in IM and after COE (p<0.05). The μTBS was not reduced after COE for the proanthocyanidins and glutaraldehyde groups, however only the proanthocyanidins treatment did not increase the NL after COE (p>0.05).



The in situ study model seems to be a suitable short-term methodology to investigate the degradation of the bonding interfaces under a more realistic condition. Under COE, the proanthocyanidins and glutaraldehyde treatments produced stable interfaces that are worth further clinical investigation.
Department of Postgraduate Program in Dentistry, CEUMA University, São Luis, MA, Brazil.
Viviane
V
Hass
Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, PR, Brazil.
Alexandra Mara
AM
de Paula
Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, PR, Brazil.
Sibelli
S
Parreiras
Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, PR, Brazil; Institute for Research in Dental Sciences, Faculty of Dentistry, University of Chile, Chile.
Mário Felipe
MF
Gutiérrez
Dentistry Academic Unit, Faculty of Medicine, Pontificia Universidad Católica de Chile, Chile.
Issis
I
Luque-Martinez
Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, PR, Brazil.
Thalita
T
de Paris Matos
Department of Postgraduate Program in Dentistry, CEUMA University, São Luis, MA, Brazil.
Matheus Coelho
MC
Bandeca
Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, PR, Brazil. Electronic address: aloguercio@hotmail.com.
Alessandro D
AD
Loguercio
Department of Oral & Craniofacial Sciences, University of Missouri-Kansas City, Kansas City, MO, USA.
Xiaomei
X
Yao
Department of Oral & Craniofacial Sciences, University of Missouri-Kansas City, Kansas City, MO, USA.
Yong
Y
Wang
Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, PR, Brazil.
Alessandra
A
Reis
D000134:Acid Etching, Dental; D003094:Collagen; D003188:Composite Resins; D001840:Dental Bonding; D003763:Dental Leakage; D003804:Dentin; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D019279:Resin Cements; D013718:Tensile Strength
D003188:Composite Resins; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D003094:Collagen
Dental bonding; Dentin collagen; Glutaraldehyde; In situ model; Proanthocyanidins; Riboflavin