TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma.
Osteosarcoma is a prevalent type of cancer and has a high metastatic ability, particularly for metastasis to the lungs. Effective treatment strategies have improved, however, the detailed molecular mechanism underlying the onset of this malignancy remains to be fully elucidated. The current study investigated the role of the tripartite motif (TRIM) family protein TRIM59 in osteosarcoma growth and metastasis. It was identified that TRIM59 was overexpressed in clinical osteosarcoma tissues and cultured osteosarcoma cell lines. In addition, the MTT assay demonstrated that in U2OS and MG63 cells, knockdown of TRIM59 by specific siRNA inhibited proliferation, whereas overexpression of TRIM59 promoted cell proliferation. Furthermore, overexpression of TRIM59 significantly increased the U2OS cell migrative and invasive abilities in a Transwell chamber assay. In addition, TRIM59 was able to negatively regulate the protein levels of P53 without significantly affecting the mRNA levels in U2OS and MG63 cells. These data suggest the oncogenic abilities of TRIM59 in osteosarcoma, which promote osteosarcoma cell proliferation, migration and invasion.
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Beijing 100730, P.R. China.
Jinqian
J
Liang
Department of Orthopaedic Surgery, Tianjin Hospital, Tianjin 300211, P.R. China.
Dan
D
Xing
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Beijing 100730, P.R. China.
Zheng
Z
Li
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Beijing 100730, P.R. China.
Jianxiong
J
Shen
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Beijing 100730, P.R. China.
Hong
H
Zhao
Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Beijing 100730, P.R. China.
Shugang
S
Li
D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008667:Metalloproteins / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000070584:Tripartite Motif Proteins; D015854:Up-Regulation
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008667:Metalloproteins; D009363:Neoplasm Proteins; C530864:TRIM59 protein, human; D000070584:Tripartite Motif Proteins