Evaluation of the Performance of a Fecal Tumor M2-PK Rapid Kit Using Stool Specimens for Detection of Colorectal Tumors.
BACKGROUND
In gastrointestinal tumors, only subunits of pyruvate kinase isoenzyme type M2 are detectable, and mainly in the dimeric form termed tumor M2-PK. A rapid test for the detection of M2-PK in stool has been developed. We evaluated the performance of the M2-PK rapid kit for the detection of colorectal tumors compared with colonoscopy


METHODS
Stool specimens (n=268) were obtained from patients who had a fecal occult blood test and colonoscopy performed for clinical evaluation or routine health checkup. Collected stool specimens were kept frozen at -20°C until testing. The fecal tumor M2-PK test was performed using a ScheBo M2-PK Quick test (ScheBo(®)•Biotech AG, Giessen, Germany)


RESULTS
A total of 236 patients were analyzed, including 87 with tubular adenoma and 126 controls. In tumor M2-PK testing, 37 of 87 tubular adenoma specimens (42.5%) and 2 of 4 sessile serrated adenoma specimens (50%) tested positive. The specificity of the M2-PK rapid test was 87.3%


CONCLUSIONS
The tumor M2-PK test demonstrated moderate sensitivity for detection of colorectal adenoma. M2-PK test could be considered as a useful point-of-care testing device for detection of colorectal tumors.
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea.
Chi Hyun
CH
Cho
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea.
Jeeyong
J
Kim
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea.
Mi Ae
MA
Jang
Department of Gastroenterology, College of Medicine, Korea University, Seoul, Korea.
Beom Jae
BJ
Lee
Department of Gastroenterology, College of Medicine, Korea University, Seoul, Korea.
Jong-Jae
JJ
Park
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea malarim@korea.ac.kr.
Chae Seung
CS
Lim
D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000201:enzymology*; D005243:Feces / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009780:Occult Blood; D011770:Pyruvate Kinase / Q000378:metabolism*; D011933:Reagent Kits, Diagnostic; D055815:Young Adult
D011933:Reagent Kits, Diagnostic; D011770:Pyruvate Kinase
M2-PK; colonoscopy; colorectal tumor; rapid test; stool