Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China.
Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.
Ren-feng
RF
Li
Xiang-qin
XQ
Tian
Yong
Y
Liu
Jing
J
Xu
Dong-yang
DY
Liu
D000595:Amino Acid Sequence; D000818:Animals; D002681:China / Q000453:epidemiology; D003001:Cloning, Molecular; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
D064370:Spike Glycoprotein, Coronavirus