Effects of resveratrol on the proliferation, apoptosis and telomerase ability of human A431 epidermoid carcinoma cells.
The aim of the present study was to investigate the effect of resveratrol on cell apoptosis, ability of telomerase and the human telomerase reverse transcriptase (hTERT) protein expression in human A431 epidermoid carcinoma cells. A431 cells were treated with different concentrations of resveratrol, and the cell appearance was then observed under a microscope. In addition, the cell proliferation was examined using an MTT assay, and the ability of telomerase was detected using telomeric repeat amplification protocol-polymerase chain reaction-ELISA. Resveratrol significantly inhibited the ability of telomerase and decreased the expression of hTERT protein in a concentration-dependent manner. In conclusion, resveratrol is capable of downregulating the expression of hTERT protein and inhibits the ability of telomerase of A431, which is an important mechanism of action of resveratrol with regard to inhibition of A431 cell proliferation.
Department of Dermatology, Traditional Chinese Medicine Hospital of Xuzhou City, Xuzhou, Jiangsu 221003, P.R. China.
Xiao-Xiang
XX
Zhai
Department of Plastic Surgery, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China.
Ji-Cun
JC
Ding
Department of Dermatology, Traditional Chinese Medicine Hospital of Xuzhou City, Xuzhou, Jiangsu 221003, P.R. China.
Zhi-Ming
ZM
Tang
Department of Dermatology, Traditional Chinese Medicine Hospital of Xuzhou City, Xuzhou, Jiangsu 221003, P.R. China.
Jing-Guo
JG
Li
Department of Dermatology, Traditional Chinese Medicine Hospital of Xuzhou City, Xuzhou, Jiangsu 221003, P.R. China.
Yong-Cong
YC
Li
Department of Dermatology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
Yue-Hua
YH
Yan
Cell Biology Laboratory, School of Basic Medicine, Shandong Medical University, Jinan, Shandong 250355, P.R. China.
Jing-Chang
JC
Sun
Department of Dermatology, Traditional Chinese Medicine Hospital of Xuzhou City, Xuzhou, Jiangsu 221003, P.R. China.
Cui-Xia
CX
Zhang
A431 cell; cell apoptosis; proliferation; resveratrol; telomerase