Sporulation properties and antimicrobial susceptibility in endemic and rare Clostridium difficile PCR ribotypes.
Increased sporulation and antibiotic resistance have been proposed to be associated with certain Clostridium difficile epidemic strains such as PCR ribotype 027. In this study we examined these properties in another widespread PCR ribotype, 014/020, in comparison to prevalent PCR ribotype 002 and a group of rarely represented PCR ribotypes. Highest sporulation was observed in 014/020 strains at 24 h, while after 72 h PCR ribotype 002 and rare PCR ribotypes formed higher total number of spores. PCR ribotype 014/020 strains exhibited slightly higher resistance to tested antimicrobials, followed by group of rare PCR ribotypes and less common PCR ribotype 002. Neither sporulation properties nor antibiotic resistance clearly differed in endemic and rare strains.
Department for Microbiology Research, Centre for Medical Microbiology, National Laboratory for Health, Environment and Food (NLZOH), Maribor, Slovenia.
Valerija
V
Zidaric
Department for Microbiology Research, Centre for Medical Microbiology, National Laboratory for Health, Environment and Food (NLZOH), Maribor, Slovenia; Faculty of Medicine, University of Maribor, Maribor, Slovenia; Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CIPKeBiP), Ljubljana, Slovenia. Electronic address: maja.rupnik@nlzoh.si.
Maja
M
Rupnik
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002645:Chickens; D016360:Clostridioides difficile / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D019353:Endemic Diseases; D004761:Enterocolitis, Pseudomembranous / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D008826:Microbial Sensitivity Tests; D021521:Ribotyping; D045483:Rivers / Q000382:microbiology; D017524:Slovenia / Q000453:epidemiology; D013171:Spores, Bacterial / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity
D000900:Anti-Bacterial Agents
Antimicrobial susceptibility; Clostridium difficile; PCR ribotype 002; PCR ribotype 014/020; Sporulation