Optogenetic control of human neurons in organotypic brain cultures.
Optogenetics is one of the most powerful tools in neuroscience, allowing for selective control of specific neuronal populations in the brain of experimental animals, including mammals. We report, for the first time, the application of optogenetic tools to human brain tissue providing a proof-of-concept for the use of optogenetics in neuromodulation of human cortical and hippocampal neurons as a possible tool to explore network mechanisms and develop future therapeutic strategies.
Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, Lund, Sweden.
My
M
Andersson
Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, Lund, Sweden.
Natalia
N
Avaliani
Lund Stem Cell Center, BMC B10 and Department of Neurosurgery, Lund University Hospital, Lund, Sweden.
Andreas
A
Svensson
Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, Lund, Sweden.
Jenny
J
Wickham
Epilepsy Clinic &Neurobiology Research Unit, Rigshospitalet, Copenhagen University Hospital, University of Copenhagen, Denmark.
Lars H
LH
Pinborg
Department of Neurosurgery, Rigshospitalet, Copenhagen University Hospital, Denmark.
Bo
B
Jespersen
Laboratory of Neural Plasticity, Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark.
Søren H
SH
Christiansen
Lund Stem Cell Center, BMC B10 and Department of Neurosurgery, Lund University Hospital, Lund, Sweden.
Johan
J
Bengzon
Laboratory of Neural Plasticity, Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark.
David P D
DP
Woldbye
Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, Lund, Sweden.
Merab
M
Kokaia
D001921:Brain / Q000166:cytology* / Q000502:physiology*; D000075402:Channelrhodopsins; D005071:Evoked Potentials / Q000528:radiation effects; D018756:GABA Antagonists / Q000494:pharmacology; D015870:Gene Expression; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D008027:Light; D009474:Neurons / Q000502:physiology*; D062308:Optogenetics; D018079:Receptors, GABA / Q000378:metabolism; D046509:Tissue Culture Techniques
D000075402:Channelrhodopsins; D018756:GABA Antagonists; D018079:Receptors, GABA; D018698:Glutamic Acid