A humanized microbiota mouse model of ovalbumin-induced lung inflammation.
There is increasing evidence for a role of early life gut microbiota in later development of asthma in children. In our recent study, children with reduced abundance of the bacterial genera Lachnospira, Veillonella, Faecalibacterium, and Rothia had an increased risk of development of asthma and addition of these bacteria in a humanized mouse model reduced airway inflammation. In this Addendum, we provide additional data on the use of a humanized gut microbiota mouse model to study the development of asthma in children, highlighting the differences in immune development between germ-free mice colonized with human microbes compared to those colonized with mouse gut microbiota. We also demonstrate that there is no association between the composition of the gut microbiota in older children and the diagnosis of asthma, further suggesting the importance of the gut microbiota-immune system axis in the first 3 months of life.
a Michael Smith Laboratories, University of British Columbia , Vancouver , British Columbia , Canada.|b Department of Microbiology & Immunology , University of British Columbia , Vancouver , British Columbia , Canada.
Marie-Claire
MC
Arrieta
c Division of Infectious Diseases, Department of Pediatrics , University of British Columbia , Vancouver , British Columbia , Canada.|d Department of Pediatrics , University of Oxford , Oxford , UK.|e Oxford University Hospitals NHS Foundation Trust , Oxford , UK.
Manish
M
Sadarangani
a Michael Smith Laboratories, University of British Columbia , Vancouver , British Columbia , Canada.|b Department of Microbiology & Immunology , University of British Columbia , Vancouver , British Columbia , Canada.
Eric M
EM
Brown
a Michael Smith Laboratories, University of British Columbia , Vancouver , British Columbia , Canada.|b Department of Microbiology & Immunology , University of British Columbia , Vancouver , British Columbia , Canada.
Shannon L
SL
Russell
f Department of Pathology and Laboratory Medicine , University of British Columbia , Vancouver , British Columbia , Canada.
Michael
M
Nimmo
g Division of Allergy and Immunology, Department of Pediatrics , University of British Columbia , Vancouver , British Columbia , Canada.
John
J
Dean
g Division of Allergy and Immunology, Department of Pediatrics , University of British Columbia , Vancouver , British Columbia , Canada.|h Child & Family Research Institute and BC Children's Hospital , Vancouver , British Columbia , Canada.
Stuart E
SE
Turvey
g Division of Allergy and Immunology, Department of Pediatrics , University of British Columbia , Vancouver , British Columbia , Canada.|h Child & Family Research Institute and BC Children's Hospital , Vancouver , British Columbia , Canada.
Edmond S
ES
Chan
a Michael Smith Laboratories, University of British Columbia , Vancouver , British Columbia , Canada.|b Department of Microbiology & Immunology , University of British Columbia , Vancouver , British Columbia , Canada.|i Department of Biochemistry and Molecular Biology , University of British Columbia , Vancouver , British Columbia , Canada.
B Brett
BB
Finlay
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004195:Disease Models, Animal; D005260:Female; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000276:immunology / Q000382:microbiology*; D005856:Germ-Free Life; D006801:Humans; D008297:Male; D051379:Mice; D010047:Ovalbumin / Q000009:adverse effects; D011014:Pneumonia / Q000209:etiology / Q000235:genetics / Q000276:immunology / Q000382:microbiology*
D010047:Ovalbumin
asthma; germ-free mice; gut microbiota; infants; lung