[Comparison Study of Oropharyngeal Microbiota in Case of Bronchial Asthma and Chronic Obstructive Pulmonary Disease in Different Severity Levels].
BACKGROUND
The result of comparative study of oropharyngeal microbiota taxonomic composition in patients with different severity level of bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD) is presented in this paper.


OBJECTIVE
To compare oropharyngeal microbiota composition in case of bronchial asthma and chronic obstructive pulmonary disease in different severity levels.


METHODS
138 patients, 50 with BA and 88 with COPD were studied. For each patient was collected anamnesis vitae, swab from the back of the throat and performed physical examination. High-throughput 16S ribosomal RNA gene sequencing and bioinformatic analysis was employed to characterize the microbial communities.


RESULTS
As a result of the study wasfound a number of differences on various taxonomic levels in microbiota's composition within group of patients with different severity level of BA and group of patients with different severity level of COPD and between those groups. COPD patients with GOLD 1-2 in comparison with GOLD 3-4 patiens are marked by prevalence of species Brevibacterium aureum, genus Scardovia, Coprococcus, Haemophilus, Moryella, Dialister, Paludibacter and decrease of Prevotella melaninogenica species. BA patients with severe uncontrolled asthma in comparison with patients which have mild persistent asthma are marked by decrease of Prevotella and increase of species Bifidobacterium longum, Prevotella nanceiensis, Neisseria cinerea, Aggregatibacter segnis and genus Odoribacter, Alloiococcus, Lactobacillus, Megasphaera, Parvimonas, Sneathia. Patient's microbiota in BA group in comparison with COPD group is characterized by the prevalence of Prevotella melaninogenica and genus Selenomonas, Granulicatella u Gemella, and decrease of Prevotella nigrescens, Haemophilus influenza and genus Aggregatibacter, Alloiococcus, Catonella, Mycoplasma, Peptoniphilus u Sediminibacterium. There are no differences between microbiota composition in case of severe uncontrolled BA and very severe COPD.


CONCLUSIONS
Lack of differences in oropharyngeal microbiota taxonomic composition between patients with severe uncontrolled BA and very severe COPD allow us to suggest a similarity of bronchopulmonary system condition in case of diseases' severe stages.
L M
LM
Ogorodova
S V
SV
Fedosenko
A S
AS
Popenko
V A
VA
Petrov
A V
AV
Tyakht
I V
IV
Saltykova
I A
IA
Deev
E S
ES
Kulikov
N A
NA
Kirillova
V M
VM
Govorun
E S
ES
Kostryukova
D000328:Adult; D000368:Aged; D001249:Asthma / Q000175:diagnosis / Q000382:microbiology* / Q000503:physiopathology; D001431:Bacteriological Techniques / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D064307:Microbiota / Q000502:physiology*; D008875:Middle Aged; D009960:Oropharynx / Q000382:microbiology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis / Q000382:microbiology* / Q000503:physiopathology; D012720:Severity of Illness Index; D013223:Statistics as Topic