Comparison of NaCl-induced programmed cell death in the obligate halophyte Cakile maritima and the glycophyte Arabidopsis thaliana.
Salinity represents one of the most important constraints that adversely affect plants growth and productivity. In this study, we aimed at determining possible differences between salt tolerant and salt sensitive species in early salt stress response. To this purpose, we subjected suspension-cultured cells from the halophyte Cakile maritima and the glycophyte Arabidopsis thaliana, two Brassicaceae, to salt stress and compared their behavior. In both species we could observe a time and dose dependent programmed cell death requiring an active metabolism, a dysfunction of mitochondria and caspase-like activation although C. maritima cells appeared less sensitive than A. thaliana cells. This capacity to mitigate salt stress could be due to a higher ascorbate pool that could allow C. maritima reducing the oxidative stress generated in response to NaCl. It further appeared that a higher number of C. maritima cultured cells when compared to A. thaliana could efficiently manage the Na(+) accumulation into the cytoplasm through non selective cation channels allowing also reducing the ROS generation and the subsequent cell death.
Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France; Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, University of Carthage-Tunis, BP 901, 2050 Hammam Lif, Tunisia.
Ibtissem
I
Ben Hamed-Laouti
Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France.
Delphine
D
Arbelet-Bonnin
Institute of Plant Sciences-Paris-Saclay (UMR 9213) Bât. 630, 91405 Orsay, France.
Linda
L
De Bont
Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France.
Bernadette
B
Biligui
Institute of Plant Sciences-Paris-Saclay (UMR 9213) Bât. 630, 91405 Orsay, France.
Bertrand
B
Gakière
Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, University of Carthage-Tunis, BP 901, 2050 Hammam Lif, Tunisia.
Chedly
C
Abdelly
Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cedria, University of Carthage-Tunis, BP 901, 2050 Hammam Lif, Tunisia.
Karim
K
Ben Hamed
Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France. Electronic address: francois.bouteau@univ-paris-diderot.fr.
François
F
Bouteau
D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D001205:Ascorbic Acid / Q000378:metabolism*; D019607:Brassicaceae / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D054712:Salinity; D055051:Salt-Tolerant Plants; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological
D000975:Antioxidants; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D012964:Sodium; D001205:Ascorbic Acid
Antioxidant; Arabidopsis thaliana; Cakile maritima; Mitochondria; Non-selective cation channels; Programmed cell death; Reactive oxygen species; Salt stress