N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation.
Appropriate metabolic interventions after hemorrhagic shock/resuscitation injury have not yet been identified. We aimed to examine the effects of fish oil on lipid metabolic intervention after hemorrhagic shock/resuscitation. Firstly, 48 C57BL/6 mice were assigned to six groups (n = 8 per group). The sham group did not undergo surgery, while mice in the remaining groups were sacrificed 1-5 days after hemorrhagic shock/resuscitation. In the second part, mice were treated with saline or fish oil (n = 8 per group) five days after injury. We determined serum triglyceride levels and liver tissues were collected and prepared for qRT-PCR or Western blot analysis. We found that triglyceride levels were increased five days after hemorrhagic shock/resuscitation, but decreased after addition of fish oil. After injury, the protein and gene expression of carnitine palmitoyltransferase 1A, fatty acid transport protein 1, and peroxisome proliferator-activated receptor-α decreased significantly in liver tissue. In contrast, after treatment with fish oil, the expression levels of these targets increased compared with those in the saline group. The present results suggest n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. This function is possibly accomplished through activating the peroxisome proliferator-activated receptor-α pathway.
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing 210002, China. zlshe1107@163.com.
Li
L
Zhang
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing 210002, China. tianfeng_nju@163.com.
Feng
F
Tian
Department of General Surgery, Jinling Hospital, Clinical College of Southern Medical University, Zhongshan East Road 305, Nanjing 210002, China. xuejingao870214@163.com.
Xuejin
X
Gao
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing 210002, China. njwangxy1978@163.com.
Xinying
X
Wang
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing 210002, China. wuchao0008@126.com.
Chao
C
Wu
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing 210002, China. lingssh@126.com.
Ning
N
Li
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Zhongshan East Road 305, Nanjing 210002, China. lijieshou1924@163.com.
Jieshou
J
Li
D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism*; D050612:Fatty Acid Transport Proteins / Q000235:genetics / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D012151:Resuscitation / Q000009:adverse effects; D012771:Shock, Hemorrhagic / Q000150:complications*; D013047:Specific Pathogen-Free Organisms; D014280:Triglycerides / Q000097:blood
D050612:Fatty Acid Transport Proteins; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D047493:PPAR alpha; D012333:RNA, Messenger; C493569:Slc27a1 protein, mouse; D014280:Triglycerides; D002334:Carnitine O-Palmitoyltransferase
fatty acid oxidation; fish oil; hemorrhagic shock; peroxisome proliferator-activated receptor