Alteration in Inflammation-related miR-146a Expression in NF-KB Signaling Pathway in Diabetic Rat Hippocampus.
OBJECTIVE
The purpose of the present study is to evaluate the expression of miR-146a gene, its adaptor genes (TRAF6, NF-KB, and IRAK1), and possible changes in the cellular signaling pathway in diabetic hippocampus tissue.


METHODS
Male Sprague-Dawley rats are randomly selected and divided into control and diabetic (n=6) groups. Diabetes induced by the single-dose injection of nicotinamide [110 mg/kg, (i.p.)], 15 min before streptozotocin (50 mg/kg; i.p.) in 12-h fasted rats. The rats are kept at the laboratory for two months. After anaesthetization, hippocampus of the rats was removed in order to measure the expression of miR-146a, NFK-B, IRAK1, and TRAF6 genes using real-time PCR and activity of NF-KB as well as amount of apoptosis rate using ELISA.


RESULTS
The results indicated a reduction in expression of miR-146a and an increase in expression of IRAK1, NF-KB, and TRAF6 genes in the hippocampus of diabetic rats compared to control. Also it reveals an increase in the activity of NF-KB and apoptosis rate in the hippocampus of diabetic rats.


CONCLUSIONS
Our results report the probability that reduction of miR-146a expression in the negative feedback loop between miR-146a and NF-KB increases NF-kB expression and thus intensifies inflammation and apoptosis in hippocampus.
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Fatemeh
F
Habibi
Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Farhad
F
Ghadiri Soufi
Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran.
Rafighe
R
Ghiasi
School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Amir Mahdi
AM
Khamaneh
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Mohammad Reza
MR
Alipour
Diabetes; Hippocampus; IRAK1; NF-KB; TRAF6; miR-146a