Mutual interactions of Pleurotus ostreatus with bacteria of activated sludge in solid-bed bioreactors.
White rot fungi are well known for their ability to degrade xenobiotics in pure cultures but few studies focus on their performance under bacterial stress in real wastewaters. This study investigated mutual interactions in co-cultures of Pleurotus ostreatus and activated sludge microbes in batch reactors and different culture media. Under the bacterial stress an increase in the dye decolorization efficiency (95 vs. 77.1 %) and a 2-fold elevated laccase activity (156.7 vs. 78.4 Ul(-1)) were observed in fungal-bacterial cultures compared to pure P. ostreatus despite a limited growth of bacteria in mixed cultures. According to 16S-rDNA analyses, P. ostreatus was able to alter the structure of bacterial communities. In malt extract-glucose medium the fungus inhibited growth of planktonic bacteria and prevented shifts in bacterial utilization of potential C-sources. A model bacterium, Rhodococcus erythropolis responded to fungal metabolites by down regulation of uridylate kinase and acetyl-CoA synthetase.
Laboratory of Environmental Biotechnology, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220, Prague, Czech Republic. ksvobod@biomed.cas.cz.
Kateřina
K
Svobodová
Laboratory of Cell Signalization, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220, Prague, Czech Republic.
Denisa
D
Petráčková
Laboratory of Cell Signalization, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220, Prague, Czech Republic.
Barbora
B
Kozická
Laboratory of Molecular Structure Characterization, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220, Prague, Czech Republic.
Petr
P
Halada
Laboratory of Environmental Biotechnology, Institute of Microbiology of the CAS, v.v.i., Vídeňská 1083, 14220, Prague, Czech Republic.
Čeněk
Č
Novotný
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D015536:Down-Regulation; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000032:analysis; D005838:Genotype; D042845:Laccase / Q000378:metabolism; D056265:Microbial Interactions; D050296:Microbial Viability; D010641:Phenotype; D020076:Pleurotus / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology
D002244:Carbon; D003066:Coenzyme A Ligases; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D042845:Laccase; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater; C101958:acetate-CoA ligase (ADP-forming)
Activated sludge; Batch reactor; Biodecolorization; Community structure; Pleurotus ostreatus; Uridylate kinase