Electrochemical estrogen screen method based on the electrochemical behavior of MCF-7 cells.
It was an urgent task to develop quick, cheap and accurate estrogen screen method for evaluating the estrogen effect of the booming chemicals. In this study, the voltammetric behavior between the estrogen-free and normal fragmented MCF-7 cell suspensions were compared, and the electrochemical signal (about 0.68V attributed by xanthine and guanine) of the estrogen-free fragmented MCF-7 cell suspension was obviously lower than that of the normal one. The electrochemistry detection of ex-secretion purines showed that the ability of ex-secretion purines of cells sharp decreased due to the removing of endogenous estrogen. The results indicated that the electrochemical signal of MCF-7 cells was related to the level of intracellular estrogen. When the level of intracellular estrogen was down-regulated, the concentrations of the xanthine and hypoxanthine decreased, which led to the electrochemical signal of MCF-7 cells fall. Based on the electrochemical signal, the electrochemical estrogen screen method was established. The estrogen effect of estradiol, nonylphenol and bisphenol A was evaluated with the electrochemical method, and the result was accordant with that of MTT assay. The electrochemical estrogen screen method was simple, quickly, cheap, objective, and it exploits a new way for the evaluation of estrogenic effects of chemicals.
College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
Jinlian
J
Li
College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
Jia
J
Song
The Affiliated First Hospital, Jiamusi University, Jiamusi 154007, China.
Sheng
S
Bi
College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
Shi
S
Zhou
College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
Jiwen
J
Cui
College of stomatology, Jiamusi University, Jiamusi 154007, China. Electronic address: liujg5550@163.com.
Jiguang
J
Liu
College of Pharmacy, Jiamusi University, Jiamusi 154007, China. Electronic address: dmwu@jmsu.edu.cn.
Dongmei
D
Wu
D001559:Benzhydryl Compounds / Q000032:analysis*; D004563:Electrochemistry; D004785:Environmental Pollutants / Q000032:analysis; D004958:Estradiol / Q000032:analysis*; D004967:Estrogens / Q000032:analysis*; D006801:Humans; D061986:MCF-7 Cells; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D004958:Estradiol; D004967:Estrogens; D010636:Phenols; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Electrochemistry method; Estrogen effect; MCF-7 cell; Purine metabolism