Dynamics of histone H2A, H4 and HS1ph during spermatogenesis with a focus on chromatin condensation and maturity of spermatozoa.
Histones and histone phosphorylation play vital roles during animal spermatogenesis and spermatozoa maturation. The dynamic distribution of histones H2A and H4 and phosphorylated H2A and H4 at serine 1 (HS1ph) was explored in mammalian and Decapoda germ cells, with a special focus on the distribution of H2A, H4 and HS1ph between mouse condensed spermatozoa chromatin and crab non-condensed spermatozoa chromatin. The distribution of histone marks was also analysed in mature spermatozoa with different chromatin structures. Histone H2A and H4 marks were closely associated with the relatively loose chromatin structure in crab spermatozoa. The significant decrease in the HS1ph signal during spermatogenesis suggests that eliminating most of these epigenetic marks in the nucleusis closely associated with spermatozoa maturity.
College of Life Sciences, Hebei University, Baoding 071000, China.|Department of Reproductive Medicine, Maternal and Child Care Service Centre, Baoding 071000, China.
Zhao-Hui
ZH
Zhang
College of Life Sciences, Hebei University, Baoding 071000, China.
Shu-Mei
SM
Mu
College of Life Sciences, Hebei University, Baoding 071000, China.
Ming-Shen
MS
Guo
College of Life Sciences, Hebei University, Baoding 071000, China.
Jiang-Li
JL
Wu
College of Life Sciences, Hebei University, Baoding 071000, China.
Yan-Qin
YQ
Li
College of Life Sciences, Hebei University, Baoding 071000, China.
Han
H
Zhang
College of Life Sciences, Hebei University, Baoding 071000, China.
Ying
Y
Wang
College of Life Sciences, Hebei University, Baoding 071000, China.
Xian-Jiang
XJ
Kang
D000818:Animals; D002454:Cell Differentiation; D042002:Chromatin Assembly and Disassembly; D003445:Crustacea; D006657:Histones / Q000378:metabolism*; D008297:Male; D051379:Mice; D008853:Microscopy; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*
D006657:Histones