The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells.
In this work, we investigated the toxic effects of tritiated water (HTO) on the cardiovascular system. We examined the role of microRNA-34a (miR-34a) in DNA damage and repair in human umbilical vein endothelial cells (HUVECs) exposed to HTO. Cell proliferation capacity was evaluated by cell counting, and miR-34a expression was detected using quantitative PCR (QT-PCR). The Comet assay and γ-H2AX immunostaining were used to measure DNA double-strand breaks (DSBs). Reverse transcription polymerase chain reaction was used to measure the expression level of c-myc messenger RNA (mRNA). The cells exposed to HTO showed significantly lower proliferation than the control cells over 3 days. The DNA damage in the HTO group was more severe than that in the control group, at each time point examined. The expression of miR-34a mimics caused increased DNA DSBs whereas that of the miR-34a inhibitor caused decreased DNA DSBs. The proliferation viability was the opposite for the miR-34a mimics and inhibitor groups. The expression levels of c-myc mRNA in cells transfected with miR-34a mimics were lower than that in cells transfected with the miR-34a-5p inhibitor, at 0.5 hours and 2 hours after transfection. In summary, miR-34a mediates HTO toxicity in HUVECs by downregulating the expression of c-myc.
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
Feng Mei
FM
Cui
Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, China.
Liang
L
Liu
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
Lu Lin
LL
Zheng
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
Guang Liang
GL
Bao
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
Yu
Y
Tu
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
Liang
L
Sun
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
Wei
W
Zhu
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
Jian Ping
JP
Cao
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
Ping Kun
PK
Zhou
Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
Qiu
Q
Chen
Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, China.
Yong Ming
YM
He
DNA damage; MiR-34a; c-myc; tritiated water