The effects of anthocyanin-rich wheat diet on the oxidative status and behavior of rats.
OBJECTIVE
To evaluate the effect of food containing anthocyanin-rich wheat on oxidative status and behavior of healthy rats.


METHODS
Twenty male rats were divided into the control and anthocyanin group. Oral glucose tolerance test was performed, and proteinuria and creatinine clearance were measured. Behavioral analysis was performed in Phenotyper cages. Serum and tissues were collected to measure the markers of oxidative stress and antioxidant status.


RESULTS
Anthocyanins significantly increased total antioxidant capacity in serum (P=0.039), decreased advanced oxidation protein products in the kidney (P=0.002), but increased thiobarbituric acid reactive substances in the kidney compared to the control group. No significant difference between the groups was found in the markers of oxidative stress in the liver and colon, as well as in renal functions and glucose metabolism. The anthocyanin group spent significantly less time in the spotlight zone of the Phenotyper cages (P=0.040), indicating higher anxiety-like behavior.


CONCLUSIONS
Food containing anthocyanin-rich wheat had positive effects on serum antioxidant status and kidney protein oxidation, but increased lipid peroxidation in the kidney and modified animal behavior related to anxiety. The molecular mechanisms leading to observed effects should be further elucidated.
Katarína Janšakova, Institute of Molecular Biomedicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia, jansakova.katarina@gmail.com.
Katarína
K
Janšakova
Janka
J
Babíčkova
Michaela
M
Havrlentova
Julius
J
Hodosy
Jan
J
Kraic
Peter
P
Celec
Lubomíra
L
Tothova
D000821:Animal Feed; D000818:Animals; D000872:Anthocyanins / Q000032:analysis*; D000975:Antioxidants / Q000378:metabolism; D001522:Behavior, Animal; D015415:Biomarkers / Q000097:blood; D004032:Diet; D005951:Glucose Tolerance Test; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014908:Triticum
D000872:Anthocyanins; D000975:Antioxidants; D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances