Molecular comparison of Slc11a1 and Slc11a2 genes of swamp- and riverine-type water buffaloes.
Solute-linked carrier 11a and 11a2 (Slc) have been associated with disease resistance and/or susceptibility across animal species. These genes have an important mechanism in the regulation against intracellular infection. This study analysed the genetic characteristic of Slc 11a and 11a2 in swamp-type and riverine-type water buffaloes to understand their immunological distinction. Characterization of Slc11a1 and Slc11a2 genes from swamp- and riverine-type water buffaloes was carried out by molecular cloning, sequencing and phylogenetic analysis. The cloned cDNA of Slc11a1 and Slc11a2 contained an open reading frame of 1647 and 1723 nucleotides, encoding 549 and 574 amino acids, respectively. Nucleotide sequence homology of both Slc11a1 and Slc11a2 had 99% in swamp and riverine type, which gives almost identical polypeptide. However, Slc11a1 and Slc11a2 have substitutions of 5 and 1 amino acid residues, correspondingly. These substitutions suggest as a potential gene markers for resistance and/or susceptibility to intracellular infection. Furthermore, phylogenetic analysis confirmed the degree of relationship between the bubaline species and justifies the distinctness of each breed by the bootstrap value generated.
Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz, Nueva Ecija, Philippines.|Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz, Philippines.
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Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz, Nueva Ecija, Philippines.|Department of Animal Science, Central Luzon State University, Science City of Muñoz, Philippines.|Scientific Career System, National Academy of Science and Technology, Department of Science and Technology, Taguig City, Philippines.
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D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D027682:Cation Transport Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000235:genetics; D006801:Humans; D010802:Phylogeny; D016415:Sequence Alignment; D053833:Wetlands; D000099173:Natural Resistance-associated Macrophage Protein 1; D000099236:Solute Carrier Family 11, Member 2
D027682:Cation Transport Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1; D000099236:Solute Carrier Family 11, Member 2