[Analysis on the laboratory examination characteristics in 22 patients with acute megakaryoblastic leukemia].
OBJECTIVE
To analyze the ultra microstructures and the expression of platelet peroxidase (PPO) of megakaryocytes from bone marrow, their clinical manifestations and laboratory characteristics in patients with acute megakaryoblastic leukemia (AMKL).


METHODS
Karyocytes from bone marrow of 22 AMKL patients were divided into two parts by lymphocyte separation liquid, one part was used to prepare the ordinary transmission electron microscope specimens to observe the morphological structures of megakaryocytes, the other was used to prepare the histochemical specimens of platelet peroxidase to analyze the positive reaction of PPO in AMKL, which were coupled with the patients' data of with bone marrow morphology, cell chemistry, and chromosome karyotype examination.


RESULTS
Megakaryocytes from 17 of 22 patients were in the first stage, less than 20 µm in diameter, the nucleis were round, the cytoplasm contained microtubules, membranous vesicles and minute dense granules, no demarcation membrane system and surface-connected canalicular system, less dense granules and α-granules; Megakaryocytes in 5 cases were mainly in the first stage, while containing second and third stage megakaryocytes; the positive rate of PPO in megakaryocytes of 22 patients was 0-80%. The primitive and naive megakaryocytes were found in bone marrow smears of 22 cases, CD41 staining of the megakaryocytes was detected in the primitive and naive megakaryocytes, and more complex chromosome karyotype anomalies were observed.


CONCLUSIONS
The majority of megakaryocytes in AMKL patients were the first stage ones, the rest were second and third stage ones, and the positive PPO reaction was significantly different. CD41 staining of the megakaryocytes was specific with complex chromosome karyotypeswere.
Institute of Hematology and Blood Diseases Hospital, CAMS & PUMG, Tianjin 300020, China.
Shuxu
S
Dong
Shixuan
S
Zhao
Ying
Y
Wang
Wen
W
Cui
Chengwen
C
Li
Yumei
Y
Chen
Xiaofan
X
Zhu
Yingchang
Y
Mi
Yongxin
Y
Ru
Jianxiang
J
Wang
D001792:Blood Platelets / Q000201:enzymology; D001853:Bone Marrow / Q000473:pathology; D002452:Cell Count; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D006801:Humans; D007621:Karyotyping; D007947:Leukemia, Megakaryoblastic, Acute / Q000175:diagnosis* / Q000473:pathology; D008533:Megakaryocytes / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D013194:Staining and Labeling
D009195:Peroxidase