The Effect of Oxidative Stress on Thawed Bulk-Sorted Red Deer Sperm.
The aims of this study were to assess the effects of the sex-sorting process on post-thaw sperm quality as well as on induced oxidative stress damage (H2 O2 0 mm = H000; H2 O2 50 mm = H050; H2 O2 100 mm = H100) and the protective action of reduced glutathione (GSH) and Trolox, when comparing sorted (BSS) and non-sorted (NS) red deer spermatozoa incubated at 37°C. Sperm samples from three stags were collected by electroejaculation and frozen. Immediately after thawing, sperm motility was higher (p < 0.05) for NS (59% ± 3.3) than BSS (36.9% ± 5.8) sperm. Furthermore, the percentage of apoptotic sperm was higher (p < 0.05) for BSS (21.6% ± 5.0) than NS sperm (14.6% ± 1.2). The presence of H2 O2 increased DNA damage in NS (H000 = 4.1% ± 0.9; H050 = 9.3% ± 0.7; and H100 = 10.9% ± 2.3), but not in BSS sperm. However, in the presence of oxidant, GSH addition improved (p < 0.05) sperm motility in both groups of sperm samples as compared to their controls (NS: 44.5 ± 4.8 vs 21.1 ± 3.9 and BSS: 33.3 ± 8.1 vs 8.9 ± 1.8). These results demonstrate that the sperm-sorting process induces sublethal effects, albeit selecting a sperm population with a chromatin more resistant to oxidative stress than that in non-sorted sperm. Moreover, addition of GSH at 1 mm may be a good choice for maintaining the quality of stressed sperm samples, unlike Trolox, which inhibited sperm motility.
SaBio IREC (CSIC-UCLM-JCCM), Albacete, Spain.
L
L
Anel-López
SaBio IREC (CSIC-UCLM-JCCM), Albacete, Spain.
O
O
García-Álvarez
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
I
I
Parrilla
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
D
D
Del Olmo
SaBio IREC (CSIC-UCLM-JCCM), Albacete, Spain.|Faculty of Pharmacy, UCLM, Albacete, Spain.
M R
MR
Fernández-Santos
SaBio IREC (CSIC-UCLM-JCCM), Albacete, Spain.
A J
AJ
Soler
SaBio IREC (CSIC-UCLM-JCCM), Albacete, Spain.
A
A
Maroto-Morales
Medianilla S.L. Finca Las Lomas, Vejer de la Frontera, Cádiz, Spain.
J A
JA
Ortiz
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
D V
DV
Alkmin
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
T
T
Tarantini
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
J
J
Roca
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
E A
EA
Martínez
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
J M
JM
Vazquez
SaBio IREC (CSIC-UCLM-JCCM), Albacete, Spain.
J J
JJ
Garde
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002839:Chromans / Q000008:administration & dosage; D015925:Cryopreservation / Q000662:veterinary; D004249:DNA Damage; D003670:Deer / Q000502:physiology*; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D005978:Glutathione / Q000008:administration & dosage; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D012743:Sex Preselection / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
D000975:Antioxidants; D002839:Chromans; D005978:Glutathione; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid