In vivo protein allylation to capture protein methylation candidates.
An approach combining in vivo protein allylation, chemical tagging and affinity enrichment was devised to capture protein methylation candidates in yeast S. cerevisiae. The study identified 167 hits, covering many proteins with known methylation events on different types of amino acid residues.
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. zhaozb@dicp.ac.cn.
Yixin
Y
Zhang
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. hanfazou@dicp.ac.cn.
Yanbo
Y
Pan
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. zhaozb@dicp.ac.cn.
Wujun
W
Liu
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. zhaozb@dicp.ac.cn.
Yongjin J
YJ
Zhou
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. hanfazou@dicp.ac.cn.
Keyun
K
Wang
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. zhaozb@dicp.ac.cn.
Lei
L
Wang
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. zhaozb@dicp.ac.cn.
Muhammad
M
Sohail
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. hanfazou@dicp.ac.cn.
Mingliang
M
Ye
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. hanfazou@dicp.ac.cn.
Hanfa
H
Zou
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China. zhaozb@dicp.ac.cn and State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China.
Zongbao K
ZK
Zhao
D008745:Methylation; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
D029701:Saccharomyces cerevisiae Proteins