Nanoband array electrode as a platform for high sensitivity enzyme-based glucose biosensing.
We describe a novel glucose biosensor based on a nanoband array electrode design, manufactured using standard semiconductor processing techniques, and bio-modified with glucose oxidase immobilized at the nanoband electrode surface. The nanoband array architecture allows for efficient diffusion of glucose and oxygen to the electrode, resulting in a thousand-fold improvement in sensitivity and wide linear range compared to a conventional electrode. The electrode constitutes a robust and manufacturable sensing platform.
NanoFlex Limited, iTac, Daresbury Laboratory, Sci-Tech Daresbury, Keckwick Lane, Daresbury WA4 4AD, United Kingdom. Electronic address: magnus.falk@nanoflex.com.
Magnus
M
Falk
NanoFlex Limited, iTac, Daresbury Laboratory, Sci-Tech Daresbury, Keckwick Lane, Daresbury WA4 4AD, United Kingdom.
Reshma
R
Sultana
NanoFlex Limited, iTac, Daresbury Laboratory, Sci-Tech Daresbury, Keckwick Lane, Daresbury WA4 4AD, United Kingdom.
Marcus J
MJ
Swann
EaStCHEM, School of Chemistry, The University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, Scotland EH9 3JJ, United Kingdom.
Andrew R
AR
Mount
NanoFlex Limited, iTac, Daresbury Laboratory, Sci-Tech Daresbury, Keckwick Lane, Daresbury WA4 4AD, United Kingdom.
Neville J
NJ
Freeman
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D057230:Limit of Detection; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010984:Platinum / Q000737:chemistry; D013499:Surface Properties
D004800:Enzymes, Immobilized; D010984:Platinum; D005949:Glucose Oxidase; D005947:Glucose
Biosensor; Glucose; Glucose oxidase; Nanoband array electrode