Effects of Cytokinin and Nitrogen on Drought Tolerance of Creeping Bentgrass.
Cytokinin (CK) is a vital plant hormone that controls many aspects of growth and development in plants. Nitrogen (N) is the indispensable macronutrient needed in plants and also one of the most important limiting factors for plant growth. This study was designed to investigate the simultaneous effects of CK and N on the visual turf quality and antioxidant metabolism of drought-stressed creeping bentgrass (Agrostis stolonifera L.). 'PennA-4' creeping bentgrass treated with trans-zeatin riboside at three rates of CK concentrations of 0, 10 and 100 μM (designated by CK0, 10, and 100) and two nitrogen rates with 2.5 and 7.5 kg N·ha-1 every 15 days (designated by low and high N) in a complete factorial arrangement was grown under two soil moisture regimes: well-watered and drought stress. Exogenous CK improved turf quality and delayed leaf wilting under drought stress, especially under high N. The grasses treated with CK10 and CK100 had lower O2- production and H2O2 concentration than those without CK treatment. The CK100 treatment enhanced the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and guaiacol peroxidase (POD) by 25%, 22%, 17% and 24%, respectively, relative to CK0. Moreover, the activity changes of the antioxidant enzyme isoforms were more significant under high N condition relative to low N condition. Our results demonstrated the beneficial impacts of CK and N on physiological reactions, especially antioxidant metabolism, and foliar application of CK at 10 or 100 μM plus 7.5 kg ha-1 N biweekly may improve drought stress resistance of creeping bentgrass.
College of Forestry, Beijing Forestry University, Beijing, 100083, P.R. China.
Zhihui
Z
Chang
College of Forestry, Beijing Forestry University, Beijing, 100083, P.R. China.
Yang
Y
Liu
College of Forestry, Beijing Forestry University, Beijing, 100083, P.R. China.
Hui
H
Dong
College of Forestry, Beijing Forestry University, Beijing, 100083, P.R. China.
Ke
K
Teng
College of Forestry, Beijing Forestry University, Beijing, 100083, P.R. China.
Liebao
L
Han
Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061, United States of America.
Xunzhong
X
Zhang
D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D003583:Cytokinins / Q000502:physiology*; D055864:Droughts; D007527:Isoenzymes / Q000378:metabolism; D009584:Nitrogen / Q000502:physiology*; D018515:Plant Leaves / Q000737:chemistry; D006109:Poaceae / Q000201:enzymology / Q000502:physiology*; D014867:Water
D000975:Antioxidants; D003583:Cytokinins; D007527:Isoenzymes; D014867:Water; D009584:Nitrogen