Antioxidative/oxidative effects and retarding osteoconductivity of ciprofloxacin-loaded porous polyvinyl alcohol/bioactive glass hybrid.
This study investigated the effect of bioglass (melting)-polyvinyl alcohol (BG (M)-PVA) and bioglass (melting)-polyvinyl alcohol-20 %ciprofloxacin (BG(M)-PVA-20Cip) in improving antioxidant activity and regenerating bone capacity. These composites were implanted in femoral condyles of ovariectomized Wistar rats and compared to that of controls groups. After the different period of implantation (15, 30, 60 and 90 days), the treatment of ovariectomized rats with BG(M)-PVA-20Cip showed a significantly higher malondialdehyde concentration when compared to that of BG(M)-PVA group. The superoxide dismutase, glutathione peroxidase and catalase in BG(M)-PVA-20Cip group showed significantly lower activities when compared to those in BG(M)-PVA group. So, BG(M)-PVA is more tolerated by organism than BG(M)-PVA-20Cip. Moreover, the alkaline phosphatase and acid phosphatase activities showed an excellent osteoinductive property of BG (M)-PVA. This property decreased with the presence of ciprofloxacin which is confirmed by histopathological analysis. Several physicochemical techniques showed a rapid reduction in Si and Na in one hand and an accelerator rise in Ca and P ions concentrations in other hand in BG(M)-PVA than in the BG(M)-PVA-20Cip. Therefore, the incorporation of ciprofloxacin in BG(M)-PVA is characterized by a prooxidant effect in oxidant-antioxidant balance at the beginning of treatment and a retard effect of formation of apatitic phase.
UMR CNRS 6226, University of Rennes 1, Campus de Beaulieu, 35042, Rennes, France.|Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, 3052, Sfax, Tunisia.|Laboratory of Sciences Material and Environment, Faculty of Sciences of Sfax, B.P. 1171, 3000, Sfax, Tunisia.
Salha
S
Boulila
UMR CNRS 6226, University of Rennes 1, Campus de Beaulieu, 35042, Rennes, France.
Hassane
H
Oudadesse
Laboratory of Histo-embryology and Cytogenetics, Medicine Faculty of Sfax University, Sfax, Tunisia.
Riadh
R
Badraoui
UMR CNRS 6226, University of Rennes 1, Campus de Beaulieu, 35042, Rennes, France.
Bertrand
B
Lefeuvre
NRC, Dokki, Cairo, Egypt.
Mostafa
M
Mabrouk
Laboratory of Biochemical, University Hospital Habib Bourguiba, Avenue El Fardous, 3029, Sfax, Tunisia.
Khansa
K
Chaabouni
NRC, Dokki, Cairo, Egypt.
Amany
A
Mostafa
Laboratory of Biochemical, University Hospital Habib Bourguiba, Avenue El Fardous, 3029, Sfax, Tunisia.
Fatma
F
Makni-Ayedi
Marrakech and CNRST, University of Cadi Ayyad, Rabat, Morocco.
Allal
A
Barroug
Laboratory of Histo-embryology and Cytogenetics, Medicine Faculty of Sfax University, Sfax, Tunisia.
Tarek
T
Rebai
Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, 3052, Sfax, Tunisia.
Abdelfattah
A
Elfeki
Laboratory of Sciences Material and Environment, Faculty of Sciences of Sfax, B.P. 1171, 3000, Sfax, Tunisia. Hafedelfeki@yahoo.com.
Hafed
H
Elfeki
D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology*; D005260:Female; D005898:Glass / Q000737:chemistry*; D005979:Glutathione Peroxidase / Q000378:metabolism; D020007:Implants, Experimental; D008855:Microscopy, Electron, Scanning; D018384:Oxidative Stress / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D017208:Rats, Wistar; D013054:Spectrophotometry, Atomic; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D002939:Ciprofloxacin; D011142:Polyvinyl Alcohol; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
Bioglass; Ciprofloxacin; Melting; Prooxidant; Retard