Down-regulation and gene hypermethylation of the 14-3-3 gamma in uterine leiomyoma.
We demonstrated that the expression of 14-3-3 gamma was lower in uterine leiomyomas compared to the adjacent normal myometrium. Here, we show that 14-3-3 gamma promoter is methylated more in leiomyomas than myometrium. The level of 14-3-3 gamma protein in leiomyomas did not change in respect to age, size, location, or the type of leiomyoma. ER-alpha, ER-beta, and PR proteins were also higher in leiomyomas and the level of these proteins negatively correlated with the level of 14-3-3 gamma protein. These results suggest that the hypermethylation of the 14-3-3 gene promoter accounts for the decreased 14-3-3 gamma in leiomyomas and that such a low level of expression may be involved in the pathogenesis of leiomyomas.
Wenxiao
W
Jiang
Hui
H
Luo
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Zhaojun
Z
Shen
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Wenwen
W
Zhang
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Aixue
A
Chen
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China, zjwzzxq@yeah.net.
Xueqiong
X
Zhu
D048948:14-3-3 Proteins / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D019175:DNA Methylation; D015536:Down-Regulation; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008597:Menstrual Cycle / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011980:Receptors, Progesterone / Q000378:metabolism; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
D048948:14-3-3 Proteins; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D011980:Receptors, Progesterone; C000610447:YWHAG protein, human