Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45.
The objective of this research was to characterize the high level of resistance to stripe that has been observed in the released wheat cultivar, Chuanmai45. A combination of classic genetic analysis, molecular and cytogenetic methods were used to characterize resistance in an F₂ population derived from Chuanmai45 and the susceptible Chuanmai42. Inheritance of resistance was shown to be conferred by two genes in Chuanmai45. Fluorescence in situ hybridization (FISH) was used along with segregation studies to show that one gene was located on a 1RS.1BL translocation. Molecular markers were employed to show that the other locus was located on chromosome 4B. The defeated gene, Yr24/26, on chromosome 1BL was present in the susceptible parent and lines that recombined this gene with the 1RS.1BL translocation were identified. The germplasm, loci, and associated markers identified in this study will be useful for application in breeding programs utilizing marker-assisted selection.
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. yangennian@126.com.
Ennian
E
Yang
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. ligr28@uestc.edu.cn.
Guangrong
G
Li
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. anzerna@126.com.
Liping
L
Li
Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. zhzhyu1982@163.com.
Zhenyu
Z
Zhang
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. yangwuyun@126.com.
Wuyun
W
Yang
Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. pengyunliang@yahoo.com.
Yunliang
Y
Peng
Institute of Agro Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. zhuyongqing68@sina.com.
Yongqing
Y
Zhu
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn.
Zujun
Z
Yang
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. garry.rosewarne@ecodev.vic.gov.au.|International Maize and Wheat Improvement Centre (CIMMYT), Apdo. Postal 6-6-41, Mexico 06600, D.F., Mexico. garry.rosewarne@ecodev.vic.gov.au.|Department of Environment and Primary Industries, 110 Natimuk Rd, Horsham, Victoria 3401, Australia. garry.rosewarne@ecodev.vic.gov.au.
Garry M
GM
Rosewarne
D015415:Biomarkers / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D017403:In Situ Hybridization; D059785:Karyotype; D010935:Plant Diseases / Q000382:microbiology*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
D015415:Biomarkers
Chuanmai45; stripe rust resistance; wheat-rye 1RS.1BL translocations