A comparative transcriptome and proteomics analysis reveals the positive effect of supplementary Ca(2+) on soybean sprout yield and nutritional qualities.
Effects of Ca(2+) on yield and nutritional qualities of soybean sprout were investigated. Ca-treated sprouts had higher yield than water-treated ones. Metabolism of selected storage materials and bioactive substances in soybean sprouts was strengthened by Ca(2+). The phytic acid and saponin content of Ca-treated soybean sprouts were lower than those of control. Supplemental Ca(2+) increased content of gamma-aminobutyric acid, isoflavones, phenolics, and vitamins, respectively. These findings indicate that supplemental Ca(2+) can increase soybean sprout yield and improve its nutritional qualities. The comparative transcriptome and proteomics between water-treated and Ca-treated soybean sprouts were studied. As consequence 1912 genes and 460 proteins were up- or down-regulated after 4days of Ca(2+) treatment. The functional classification of these differentially expressed genes and proteins indicated their connection with primary/secondary metabolic pathways, ion transport, signal transduction, and transcriptional regulation. The results obtained here will enable to understand how changes in yield and nutritional quality are regulated by extra Ca(2+) in soybean sprouts.



In this study, a total of 1912 genes and 460 proteins involved in the growth, storage material decomposition, and bioactive substance synthesis in soybean sprouts after treated with Ca(2+) were identified. This is the first report of a comprehensive transcriptomic and proteomic analysis of soybean sprout in response to supplemental Ca(2+).
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Xinkun
X
Wang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Runqiang
R
Yang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Yulin
Y
Zhou
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China. Electronic address: zhenxingu@126.com.
Zhenxin
Z
Gu
D002118:Calcium / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000187:drug effects*; D009753:Nutritive Value; D010940:Plant Proteins / Q000032:analysis; D040901:Proteomics; D013025:Glycine max / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D059467:Transcriptome / Q000187:drug effects*
D010940:Plant Proteins; D002118:Calcium
Nutritional characteristics; Soybean sprout; Supplemental Ca(2+); Yield