Chitin nanofibrils suppress skin inflammation in atopic dermatitis-like skin lesions in NC/Nga mice.
We evaluated the effect of chitin nanofibril (CNF) application via skin swabs on an experimental atopic dermatitis (AD) model. AD scores were lower, and hypertrophy and hyperkeratosis of the epidermis were suppressed after CNF treatment. Furthermore, inflammatory cell infiltration in both the epidermis and dermis was inhibited. CNFs also attenuated histological scores. The suppressive effects of CNFs were equal to those of corticosteroid application; however, chitin did not show these effects. CNF application might have anti-infllammatory effects via suppression of the activation of nuclear factor-kappa B, cyclooxygenase-2, and inducible nitric oxide synthase. In an early-stage model of experimental AD, CNFs suppressed AD progression to the same extent as corticosteroids. They also suppressed skin inflammation and IgE serum levels. Our findings indicate that CNF application could aid in the prevention or treatment of AD skin lesions.
Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: jastam0ment@gmail.com.
Ryotaro
R
Izumi
Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: kazu-azuma@muses.tottori-u.ac.jp.
Kazuo
K
Azuma
Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: h-izawa@chem.tottori-u.ac.jp.
Hironori
H
Izawa
Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University, Tottori 680-8550, Japan. Electronic address: morimoro@chem.tottori-u.ac.jp.
Minoru
M
Morimoto
Regional Industrial Academic Cooperation, Tottori University, Tottori 680-0833, Japan. Electronic address: nagashima@cjrd.tottori-u.ac.jp.
Masaaki
M
Nagashima
Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: tosaki@muses.tottori-u.ac.jp.
Tomohiro
T
Osaki
Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: tsuka@muses.tottori-u.ac.jp.
Takeshi
T
Tsuka
Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: imagawat@muses.tottori-u.ac.jp.
Tomohiro
T
Imagawa
Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: taro@muses.tottori-u.ac.jp.
Norihiko
N
Ito
Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: yokamoto@muses.tottori-u.ac.jp.
Yoshiharu
Y
Okamoto
Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: saimoto@chem.tottori-u.ac.jp.
Hiroyuki
H
Saimoto
Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: sifuku@chem.tottori-u.ac.jp.
Shinsuke
S
Ifuku
D000818:Animals; D002686:Chitin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D003876:Dermatitis, Atopic / Q000628:therapy*; D007249:Inflammation / Q000628:therapy; D051379:Mice; D057139:Nanofibers; D012867:Skin / Q000187:drug effects
D002686:Chitin
Atopic dermatitis; Chitin nanofibril; Corticosteroids; Cyclooxygenase-2; Inducible nitric oxide synthase; Nuclear factor-kappa B