Determination of microcystin-LR in clams (Tapes decussatus) of two Sardinian coastal ponds (Italy).
The presence of microcystin-LR (MC-LR) was monitored in Tapes decussatus harvested in two Sardinian ponds (Cabras and Tortolì, Italy) in spring and summer. After solid phase extraction, samples were analyzed using a screening enzyme-linked immunosorbent assay (ELISA) followed by a liquid chromatographic coupled to tandem mass spectrometer (LC-MS/MS) analysis. Results obtained through the ELISA test showed the presence of microcystins with a maximum concentration in August for Cabras pond (0.55ng/g) and in September for Tortolì pond (0.85ng/g). The LC-MS/MS analysis did not confirm the presence of MC-LR suggesting that results obtained with the ELISA technique could be due to the presence of other microcystins. According to the tolerable daily intake suggested by the World Health Organization, these results hint that clams harvested in these ponds are safe for human health. These data can contribute to enrich the knowledge about the healthiness of Sardinian ponds and of their products.
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
Tiziana
T
Sedda
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy. Electronic address: ebaralla@uniss.it.
Elena
E
Baralla
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
Maria Vittoria
MV
Varoni
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
Valeria
V
Pasciu
Experimental Zooprophylattic Institute of Sardinia, via Duca degli Abruzzi, 07100 Sassari, Italy.
Giuseppa
G
Lorenzoni
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
Maria Piera
MP
Demontis
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D007558:Italy; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D061005:Ponds / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; C057862:cyanoginosin LR
Microcystin; Ponds; Sardinia; Tapes decussatus