Metabolites from invasive pests inhibit mitochondrial complex II: A potential strategy for the treatment of human ovarian carcinoma?
The red pigment caulerpin, a secondary metabolite from the marine invasive green algae Caulerpa cylindracea can be accumulated and transferred along the trophic chain, with detrimental consequences on biodiversity and ecosystem functioning. Despite increasing research efforts to understand how caulerpin modifies fish physiology, little is known on the effects of algal metabolites on mammalian cells. Here we report for the first time the mitochondrial targeting activity of both caulerpin, and its closely related derivative caulerpinic acid, by using as experimental model rat liver mitochondria, a system in which bioenergetics mechanisms are not altered. Mitochondrial function was tested by polarographic and spectrophotometric methods. Both compounds were found to selectively inhibit respiratory complex II activity, while complexes I, III, and IV remained functional. These results led us to hypothesize that both algal metabolites could be used as antitumor agents in cell lines with defects in mitochondrial complex I. Ovarian cancer cisplatin-resistant cells are a good example of cell lines with a defective complex I function on which these molecules seem to have a toxic effect on proliferation. This provided novel insight toward the potential use of metabolites from invasive Caulerpa species for the treatment of human ovarian carcinoma cisplatin-resistant cells.
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy. Electronic address: alessandra.ferramosca@unisalento.it.
Alessandra
A
Ferramosca
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy.
Annalea
A
Conte
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy.
Flora
F
Guerra
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy.
Serena
S
Felline
Dipartimento di Farmacia, Università di Napoli Federico II, Napoli, Italy.
Maria Grazia
MG
Rimoli
Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Pozzuoli, Italy.
Ernesto
E
Mollo
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy.
Vincenzo
V
Zara
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy; Stazione Zoologica Anton Dohrn, Napoli, Italy.
Antonio
A
Terlizzi
D019069:Cell Respiration / Q000187:drug effects / Q000502:physiology; D000460:Chlorophyta / Q000378:metabolism*; D042963:Electron Transport Complex II / Q000037:antagonists & inhibitors*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007211:Indoles / Q000008:administration & dosage* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000628:therapy
D007211:Indoles; D024101:Mitochondrial Proteins; C000392:caulerpin; D042963:Electron Transport Complex II
Algal metabolites; Caulerpa; Marine biological invasions; Mitochondria; Ovarian cancer cells; Respiration