Flavobacterium tyrosinilyticum sp. nov., isolated from the rhizosphere of wild strawberry.
A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterial strain, designated THG-DN8.8T, was isolated from the rhizosphere of a wild strawberry plant, located on Gyeryong Mountain, Daejeon, Republic of Korea. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Flavobacterium and was related most closely to Flavobacterium banpakuense 15F3T(97.7 % sequence similarity), Flavobacterium ginsenosidimutans THG-01T(97.3 %), Flavobacterium ginsengiterrae DCY55T(96.9 %) and Flavobacterium chungbukense CS100T(96.7 %). Catalase and oxidase tests were positive. Levels of DNA-DNA relatedness with its phylogenetically closest neighbours were below 40.0 %. The DNA G+C content was determined to be 39.1 mol%. Strain THG-DN8.8Talso was found to be able to grow at 4-33 °C, with 0-2 % (w/v) NaCl and at pH 5.5-9.5. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. Menaquinone-6 (MK-6) was the dominant respiratory quinone, and phosphatidylethanolamine and phosphatidyldiethanolamine were the main polar lipids. On the basis of the data presented, strain THG-DN8.8Trepresents a novel species, for which the name Flavobacterium tyrosinilyticum sp. nov. is proposed. The type strain is THG-DN8.8T(=KCTC 42726T=CCTCC AB 2015295T).
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, South Korea.
Juan
J
Du
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, South Korea.
Tae-Hoo
TH
Yi
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D031985:Fragaria / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6
16S rRNA; Flavobacterium tyrosinilyticum; rhizosphere