High resolution ultrasound and photoacoustic imaging of single cells.
High resolution ultrasound and photoacoustic images of stained neutrophils, lymphocytes and monocytes from a blood smear were acquired using a combined acoustic/photoacoustic microscope. Photoacoustic images were created using a pulsed 532 nm laser that was coupled to a single mode fiber to produce output wavelengths from 532 nm to 620 nm via stimulated Raman scattering. The excitation wavelength was selected using optical filters and focused onto the sample using a 20× objective. A 1000 MHz transducer was co-aligned with the laser spot and used for ultrasound and photoacoustic images, enabling micrometer resolution with both modalities. The different cell types could be easily identified due to variations in contrast within the acoustic and photoacoustic images. This technique provides a new way of probing leukocyte structure with potential applications towards detecting cellular abnormalities and diseased cells at the single cell level.
Department of Physics, Ryerson University, Toronto, Ontario M5B2K3, Canada; Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Ryerson University and St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada; Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada.
Eric M
EM
Strohm
Department of Physics, Ryerson University, Toronto, Ontario M5B2K3, Canada; Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Ryerson University and St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada; Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada.
Michael J
MJ
Moore
Department of Physics, Ryerson University, Toronto, Ontario M5B2K3, Canada; Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Ryerson University and St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada; Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, Ontario, M5B1T8, Canada.
Michael C
MC
Kolios
Acoustic microscopy; Blood smear; Leukocytes; Photoacoustic microscopy