Chronic Exposure to Perfluorooctane Sulfonate Reduces Lifespan of Caenorhabditis elegans Through Insulin/IGF-1 Signaling.
Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant. Although multiple adverse effects of PFOS have been demonstrated, whether PFOS can accelerate aging and affect animal longevity remains unknown. In Caenorhabditis elegans, we found that a 50 h exposure to 0.2-200 µM PFOS reduced lifespan in a concentration dependent manner. In transgenic nematodes, lifespans are affected by mutations of daf-16, daf-2 or age-1 genes, which are related to the Insulin/IGF-1 Signaling pathway (IIS). PFOS exposure caused an additional reduction in average lifespan in daf-2(e1370) and daf-16b(KO) nematodes. In contrast, daf-16(mu86) nematodes showed no additional reduction with PFOS exposure and age-1(hx546) mutants did not exhibit a reduction in lifespan with PFOS exposure, compared with wildtype nematodes. Overall, our findings demonstrate that PFOS exposure accelerates aging and shortens longevity of animals. The PFOS-induced effect may involve genes of the IIS pathway, particularly daf-16 and age-1.
Lab of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, 500# DongChuang RD, Shanghai, 200241, China.
Tiantian
T
Xu
Lab of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, 500# DongChuang RD, Shanghai, 200241, China.
Ping
P
Li
Lab of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, 500# DongChuang RD, Shanghai, 200241, China.
Siyu
S
Wu
Lab of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, 500# DongChuang RD, Shanghai, 200241, China.
Dan
D
Li
Lab of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, 500# DongChuang RD, Shanghai, 200241, China.
Jingxuan
J
Wu
Department of Biology, Vassar College, Poughkeepsie, NY, 12604, USA.
Kathleen M
KM
Raley-Susman
Lab of Toxicology, School of Ecological and Environmental Sciences, East China Normal University, 500# DongChuang RD, Shanghai, 200241, China. dfhe@des.ecnu.edu.cn.
Defu
D
He
D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D004785:Environmental Pollutants / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D017238:Genes, Helminth; D007328:Insulin / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology*; D008136:Longevity / Q000187:drug effects; D009154:Mutation; D015398:Signal Transduction
D017738:Alkanesulfonic Acids; D029742:Caenorhabditis elegans Proteins; D004785:Environmental Pollutants; D005466:Fluorocarbons; D007328:Insulin; D007334:Insulin-Like Growth Factor I; C076994:perfluorooctane sulfonic acid
Age-1; Caenorhabditis elegans; Daf-16; IIS signaling; Lifespan; Perfluorooctane sulfonate