Comparative genomic hybridization in detection of DNA changes in canine lymphomas.
In this study, chromosomal imbalances in tumor tissues (lymphomas) and nucleotide changes in tumor suppressor TP53 were studied in a Bernese Mountain dog bitch and a cross breed bitch. Using comparative genomic hybridization, numerous chromosomal rearrangements were detected, which indicated the heterogeneity in tumor growth: in the cross breed bitch, a deletion on the chromosome 9, and duplications on chromosomes 5, 8 and 17 have been found. In the Bernese Mountain Dog bitch, losses on chromosomes 1, 5, 8, 12, 18, 22, 27, 29 and gains on chromosomes 1, 2, 9, 11, 15, 16, 18, 20, 23, 24, 25, 28, 29, 30, 34, 36, 37 and 38 were identified. With the sequencing of the TP53 gene, one silent mutation, transition A/G at position 138 in exon 5 was detected, without changing the amino acid.
Institute of Genetics, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
Monika
M
Drážovská
Institute of Genetics, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
Katarína
K
Šiviková
Institute of Genetics, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
Ján
J
Dianovský
Department of Genetics and Reproduction, CEITEC - Veterinary Research Institute, Brno, Czech Republic.
Miroslav
M
Horňák
D000818:Animals; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000235:genetics*; D055028:Comparative Genomic Hybridization / Q000379:methods*; D004247:DNA / Q000235:genetics*; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005091:Exons / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology / Q000662:veterinary*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
D004247:DNA
PCR technique; TP53 gene; canine lymphomas; chromosomal imbalances; comparative genomic hybridization (CGH)