High-efficiency antibody discovery achieved with multiplexed microscopy.
The analysis of secreted antibody from large and diverse populations of B cells in parallel at the clonal level can reveal desirable antibodies for diagnostic or therapeutic applications. By immobilizing B cells in microdroplets with particulate reporters, decoding and isolating them in a microscopy environment, we have recovered panels of antibodies with rare attributes to therapeutically relevant targets. The ability to screen up to 100 million cells in a single experiment can be fully leveraged by accessing primary B-cell populations from evolutionarily divergent species such as chickens.
Crystal Bioscience, Emeryville, CA, USA.
Shelley
S
Mettler Izquierdo
Crystal Bioscience, Emeryville, CA, USA.
Stephanie
S
Varela
Crystal Bioscience, Emeryville, CA, USA.
Minha
M
Park
Crystal Bioscience, Emeryville, CA, USA.
Ellen J
EJ
Collarini
Crystal Bioscience, Emeryville, CA, USA.
Daniel
D
Lu
Crystal Bioscience, Emeryville, CA, USA.
Shreya
S
Pramanick
Integral Molecular, Philadelphia, PA, USA.
Joseph
J
Rucker
Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
Lucia
L
Lopalco
Crystal Bioscience, Emeryville, CA, USA.
Rob
R
Etches
Crystal Bioscience, Emeryville, CA, USA bharriman@crystalbioscience.com.
William
W
Harriman
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D016466:CHO Cells; D045744:Cell Line, Tumor; D002645:Chickens; D003412:Cricetulus; D055808:Drug Discovery / Q000379:methods; D006801:Humans; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D019169:Jurkat Cells; D019713:Receptors, CCR5 / Q000276:immunology*; D058477:Receptors, Purinergic P2X3 / Q000276:immunology*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D019713:Receptors, CCR5; D058477:Receptors, Purinergic P2X3; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand
DR4 DR5; GPCR; antibody discovery; chicken monoclonal antibody; ion channel; single B cell cloning