Description of genomic islands associated to the multidrug-resistant Pseudomonas aeruginosa clone ST277.
Multidrug-resistant Pseudomonas aeruginosa clone ST277 is disseminated in Brazil where it is mainly associated with the presence of metallo-β-lactamase SPM-1. Furthermore, it carries the class I integron In163 and a 16S rRNA methylase rmtD that confers aminoglycoside resistance. To analyze the genetic characteristics that might be responsible for the success of this endemic clone, genomes of four P. aeruginosa strains that were isolated in distinct years and in different Brazilian states were sequenced. The strains differed regarding the presence of the genes blaSPM-1 and rmtD. Genomic comparisons that included genomes of other clones that have spread worldwide from this species were also performed. These analyses revealed a 763,863bp region in the P. aeruginosa chromosome that concentrates acquired genetic structures comprising two new genomic islands (PAGI-13 and PAGI-14), a mobile element that could be used for ST277 fingerprinting and a recently reported Integrative and Conjugative Element (ICE) associated to blaSPM-1. The genetic elements rmtD and In163 are inserted in PAGI-13 while PAGI-14 has genes encoding proteins related to type III restriction system and phages. The data reported in this study provide a basis for a clearer understanding of the genetic content of clone ST277 and illustrate the mechanisms that are responsible for the success of these endemic clones.
Laboratório de Pesquisa em Infecção Hospitalar, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, 21040-360 Rio de Janeiro, Rio de Janeiro, Brazil; Laboratório de Biologia Computacional e Sistemas, Oswaldo Cruz Instituto, Fiocruz, Avenida Brasil 4365, Manguinhos, 21040-360 Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: melisechaves@gmail.com.
Melise Chaves
MC
Silveira
Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, Boulevard Vinte e Oito de Setembro, 87, fundos, 4 andar, Vila Isabel, 20551-030 Rio de Janeiro, Rio de Janeiro, Brazil.
Rodolpho Mattos
RM
Albano
Laboratório de Pesquisa em Infecção Hospitalar, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, 21040-360 Rio de Janeiro, Rio de Janeiro, Brazil.
Marise Dutra
MD
Asensi
Laboratório de Pesquisa em Infecção Hospitalar, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, 21040-360 Rio de Janeiro, Rio de Janeiro, Brazil.
Ana Paula D'Alincourt
AP
Carvalho-Assef
D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000737:chemistry; D002999:Clone Cells; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D044404:Genomic Islands; D006801:Humans; D032023:Integrons; D008780:Methyltransferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D008780:Methyltransferases; C476155:beta-lactamase SPM-1, Pseudomonas aeruginosa; D001618:beta-Lactamases
Genomic comparisons; Multidrug resistant clone; Pseudomonas aeruginosa