Polysaccharide peptides from Coriolus versicolor exert differential immunomodulatory effects on blood lymphocytes and breast cancer cell line MCF-7 in vitro.
The protein-bound polysaccharides (PBP), isolated from Coriolus versicolor (CV) fungus, are considered as natural compounds with potential therapeutic applications. The immunopotentiating and antitumor activity of polysaccharopeptides has been previously examined, however similar findings could not be achieved. The source of PBP, variations in extraction process as well as environmental factors seems to affect the biological properties of these active CV components. Since further analysis are needed to draw more definite conclusion, the present study aimed to investigate the immunomodulatory properties of the PBP extract, isolated from commercially available capsules of C. versicolor. Our results revealed that the effect mediated by PBP extract depends on the target cells. We reported that the polysaccharopeptides induced a significant decrease in breast cancer MCF-7 cells growth, which was TNF-α-dependent phenomenon. Interestingly, the level of two others cytokines, IL-1β and IL-6 was not affected. On the other hand, in this study we noticed that protein-bound polysaccharides extracted from CV significantly augmented the proliferative response of blood lymphocytes in a time-dependent manner, which was associated with IL-6 and IL-1β mRNA upregulation. Moreover we found that the cells response to PBP stimuli might be inversely related to its concentration.
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland. Electronic address: m_kowalczewska@umk.pl.
Małgorzata
M
Kowalczewska
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wilenska 4 Street, 87-100 Torun, Poland. Electronic address: piotrowski_jak@umk.pl.
Jakub
J
Piotrowski
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland. Electronic address: tomaszj@umk.pl.
Tomasz
T
Jędrzejewski
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Lwowska 1 Street, 87-100 Torun, Poland. Electronic address: wkozak@umk.pl.
Wiesław
W
Kozak
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D061986:MCF-7 Cells / Q000187:drug effects*; D011509:Proteoglycans / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
D000970:Antineoplastic Agents; D016207:Cytokines; D007155:Immunologic Factors; D011509:Proteoglycans; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C093216:polysaccharide peptide
Coriolus versicolor; Cytokine; Lymphocytes; MCF-7 cells; MTT test; Protein-bound polysaccharides; qRT-PCR