CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects.
The increasing burden of the world population on agriculture requires the development of more robust crops. Dissecting the basic biology that underlies plant development and stress responses will inform the design of better crops. One powerful tool for studying plants at the molecular level is the RNA-programmed genome editing system composed of a clustered regularly interspaced short palindromic repeats (CRISPR)-encoded guide RNA and the nuclease Cas9. Here, some of the recent advances in CRISPR/Cas9 technology that have profound implications for improving the study of plant biology are described. These tools are also paving the way towards new horizons for biotechnologies and crop development.
Department of Biology, Thomas Harriot College of Arts and Sciences, East Carolina University, Greenville, NC, 27858, USA.
Joseph W
JW
Paul
Department of Biology, Thomas Harriot College of Arts and Sciences, East Carolina University, Greenville, NC, 27858, USA. qiy@ecu.edu.
Yiping
Y
Qi
D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D018556:Crops, Agricultural / Q000235:genetics; D000072669:Gene Editing / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D008957:Models, Genetic; D010944:Plants / Q000235:genetics*; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics; D015203:Reproducibility of Results
D000094704:RNA, Guide, CRISPR-Cas Systems
CRISPR; Cas9; Gene targeting; Genome editing; Plant biotechnology; Synthetic biology; Transcriptional regulation