[Establishment of multiplex PCR method for rapid detection of nontuberculous mycobacteriums infection in the hand].
OBJECTIVE
To establish a multiplex polymerase chain reaction (mPCR) method with high sensitivity and specificity for rapid detection of common nontuberculous mycobacterium(NTM) infection in the hand.


METHODS
Application of primer design software to the mycobacterium marinum, mycobacterium avium, mycobacterium kansasii and mycobacterium fortuitum, the specific gene sequences were used to design construction of multiplex PCR and detection of DNA from the non tuberculous mycobacterial standard strains of each bacterium of single PCR and multiplex DNA accuracy and sequence contrast evaluation to verify the specificity of multiple PCR primers.26 clinical specimens were identified by this method.


RESULTS
Detection of 26 cases of clinical samples, positive detection of more than 7/8, the identification time is shorter than the traditional method.


CONCLUSIONS
The research method can be rapid, specific, sensitive and effective to detect the common hand of mycobacterium tuberculosis infection, can be used for clinical identification of mycobacterium tuberculosis infection.
The Fifth Affiliated Hospital ZunYi Medical College, Zhuhai 519100, China.
L L
LL
Liu
S J
SJ
Chen
H
H
Long
C
C
Wang
T Y
TY
Cao
Z X
ZX
Hu
D
D
Wu
D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009161:Mycobacterium / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D017931:DNA Primers