Searching for Common Mammalian Retroviruses in Pediatric Idiopathic Diseases.
Mammalian retroviruses cause a variety of diseases in their hosts, including hematological and immunodeficiency disorders. Both human T-cell leukemia (HTLV) and human immunodeficiency (HIV) viruses originated from several independent zoonotic transmissions, indicating that cross-species transmissions from animal to humans may still occur. Thus, as the risk for retroviral transmissions from animals to humans increase, we investigated whether mammalian retroviruses are involved in selected pediatric idiopathic diseases whose symptoms evoke retroviral infections. Blood samples, sera, and synovial fluids, or bone marrow cells were collected from pediatric patients under 18 years of age with different autoimmune idiopathic diseases. Overall, we screened clinical samples from 110 children using sensitive nested and semi-nested PCR strategies targeting env genes, and a C-type retrovirus reverse transcriptase (RT) activity kit. All clinical samples were free of retroviral signatures, indicating the unlikelihood of an etiological role of the retroviruses we assessed in the pediatric diseases we tested.
Institut de Génétique Moléculaire de Montpellier (IGMM), UMR5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, Université de Montpellier, Place Eugène Bataillon, 34095 Montpellier cedex 5, France. e-jeziorski@chu-montpellier.fr.|Hôpital Arnaud de Villeneuve, Service de Pédiatrie générale, infectiologie et immunologie clinique, Centre Hospitalier Régional Universitaire de Montpellier, 371, avenue du Doyen Gaston Giraud, 34295 Montpellier cedex 5, France. e-jeziorski@chu-montpellier.fr.
Eric
E
Jeziorski
Laboratoire de virologie, Centre Hospitalier Régional Universitaire de Montpellier, 80 avenue A. Fliche, 34295 Montpellier cedex 5, France. v-foulongne@chu-montpellier.fr.
Vincent
V
Foulongne
Hôpital Arnaud de Villeneuve, Service de Pédiatrie générale, infectiologie et immunologie clinique, Centre Hospitalier Régional Universitaire de Montpellier, 371, avenue du Doyen Gaston Giraud, 34295 Montpellier cedex 5, France. c-ludwig@chu-montpellier.fr.
Catherine
C
Ludwig
Service de Chirurgie Orthopédique, Centre Hospitalier Régional Universitaire de Montpellier, 371 avenue du Doyen Gaston Giraud, 34295 Montpellier cedex 5, France. d-louahemm_sabah@chu-montpellier.fr.
Djamel
D
Louhaem
Hôpital Arnaud de Villeneuve, Service de Pédiatrie générale, infectiologie et immunologie clinique, Centre Hospitalier Régional Universitaire de Montpellier, 371, avenue du Doyen Gaston Giraud, 34295 Montpellier cedex 5, France. m-rodiere@chu-montpellier.fr.
Michel
M
Rodiere
Institut de Génétique Moléculaire de Montpellier (IGMM), UMR5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, Université de Montpellier, Place Eugène Bataillon, 34095 Montpellier cedex 5, France. marc.sitbon@igmm.cnrs.fr.
Marc
M
Sitbon
Institut de Génétique Moléculaire de Montpellier (IGMM), UMR5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, Université de Montpellier, Place Eugène Bataillon, 34095 Montpellier cedex 5, France. valerie.courgnaud@igmm.cnrs.fr.
Valérie
V
Courgnaud
D000293:Adolescent; D001327:Autoimmune Diseases / Q000209:etiology* / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D015686:Gene Products, env / Q000235:genetics; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012190:Retroviridae / Q000235:genetics / Q000302:isolation & purification*; D012192:Retroviridae Infections / Q000473:pathology* / Q000821:virology*
D015686:Gene Products, env; D012194:RNA-Directed DNA Polymerase
autoimmune idiopathic disease; emergent viruses; env gene; pediatric patients; polymerase chain reaction; retroviruses