Global proteome profiling of dental cementum under experimentally-induced apposition.
Dental cementum (DC) covers the tooth root and has important functions in tooth attachment and position. DC can be lost to disease, and regeneration is currently unpredictable due to limited understanding of DC formation. This study used a model of experimentally-induced apposition (EIA) in mice to identify proteins associated with new DC formation. Mandibular first molars were induced to super-erupt for 6 and 21days after extracting opposing maxillary molars. Decalcified and formalin-fixed paraffin-embedded mandible sections were prepared for laser capture microdissection. Microdissected protein extracts were analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and the data submitted to repeated measure ANOVA test (RM-ANOVA, alpha=5%). A total of 519 proteins were identified, with 97 (18.6%) proteins found exclusively in EIA sites and 50 (9.6%) proteins exclusively expressed in control sites. Fifty six (10.7%) proteins were differentially regulated by RM-ANOVA (p<0.05), with 24 regulated by the exclusive effect of EIA (12 proteins) or the interaction between EIA and time (12 proteins), including serpin 1a, procollagen C-endopeptidase enhancer, tenascin X (TNX), and asporin (ASPN). In conclusion, proteomic analysis demonstrated significantly altered protein profile in DC under EIA, providing new insights on DC biology and potential candidates for tissue engineering applications.



Dental cementum (DC) is a mineralized tissue that covers the tooth root surface and has important functions in tooth attachment and position. DC and other periodontal tissues can be lost to disease, and regeneration is currently unpredictable due to lack of understanding of DC formation. This study used a model of experimentally-induced apposition (EIA) in mice to promote new cementum formation, followed by laser capture microdissection (LCM) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) proteomic analysis. This approach identified proteins associated with new cementum formation that may be targets for promoting cementum regeneration.
Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas, São Paulo, Brazil.
Cristiane R
CR
Salmon
Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas, São Paulo, Brazil.
Ana Paula O
AP
Giorgetti
National Biosciences Laboratory, Brazilian Synchrotron Light Laboratory, Campinas, SP, Brazil.
Adriana Franco
AF
Paes Leme
National Biosciences Laboratory, Brazilian Synchrotron Light Laboratory, Campinas, SP, Brazil.
Romênia R
RR
Domingues
Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas, São Paulo, Brazil.
Enilson Antonio
EA
Sallum
Technical Section of Informatics, ESALQ-University of São Paulo, Piracicaba, SP, Brazil.
Marcelo C
MC
Alves
Biosciences Division, College of Dentistry, Ohio State University, Columbus, OH, United States.
Tamara N
TN
Kolli
Biosciences Division, College of Dentistry, Ohio State University, Columbus, OH, United States.
Brian L
BL
Foster
Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, State University of Campinas, São Paulo, Brazil. Electronic address: nociti@unicamp.br.
Francisco H
FH
Nociti
D000818:Animals; D002853:Chromatography, Liquid; D003739:Dental Cementum / Q000502:physiology*; D020869:Gene Expression Profiling; D051379:Mice; D023421:Models, Animal; D010513:Periodontal Ligament; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D012038:Regeneration; D053719:Tandem Mass Spectrometry; D014092:Tooth Root
D011506:Proteins; D020543:Proteome
Dental cementum; Periodontal ligament; Proteomic analysis; Tissue apposition