Inhibition of postsurgical adhesions by methylene blue-loaded nanofibers versus cast film matrices.
In the quest for barrier membranes for the prevention of post-surgical tissue adhesions, polymer matrices may provide a platform of biomaterials with versatile properties. However, the relationship between the anti-adhesion effects of different polymer matrices and their physicochemical and structural properties is not yet adequately understood. In a preclinical study using a rat cecum model, we directly compared the anti-adhesion potential of polyhydroxybutyrate (PHB) electrospun nanofibrous versus cast film matrices loaded with methylene blue (MB) as antioxidant adhesion inhibitor. PHB retained MB presumably forming MB-bioactivated matrices. In the preclinical study, quantitative morphologic assessment in addition to histopathologic and SEM examinations 14 days post-surgery indicated that plain PHB NFs and MB-PHB NFs, moderately enhanced cecal wall healing and inhibited adhesion formation. In contrast, reshaping PHB as cast films, significantly enhanced healing, reduced adhesion bands and prevented inter-visceral adhesions. Cast films also inhibited tissue attachment to the matrix recovered 14 days post-surgery. Both PHB matrix types reduced tissue inflammation. Despite tissue anti-adhesion potential of individual matrix components, modulation of the micro-architectural properties generated polymer barriers with varying tissue anti-adhesion and healing potentials, the MB-loaded cast film achieving the best outcome.
a Faculty of Pharmacy, Department of Pharmaceutics , Alexandria University , Alexandria , Egypt.
Nesma
N
El-Sayed
a Faculty of Pharmacy, Department of Pharmaceutics , Alexandria University , Alexandria , Egypt.
Sally
S
Galal
b Faculty of Pharmacy, Department of Pharmacology and Toxicology , Alexandria University , Alexandria , Egypt.
Hanan
H
El-Gowelli
a Faculty of Pharmacy, Department of Pharmaceutics , Alexandria University , Alexandria , Egypt.
Labiba
L
El-Khordagui
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002432:Cecum / Q000473:pathology / Q000601:surgery; D005260:Female; D008751:Methylene Blue / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000494:pharmacology; D000091402:Prohibitins; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D000267:Tissue Adhesions / Q000517:prevention & control*; D014945:Wound Healing
D001672:Biocompatible Materials; C000717332:Phb protein, rat; D011108:Polymers; D000091402:Prohibitins; D008751:Methylene Blue
Polyhydroxybutyrate; cast film; methylene blue; nanofibers; rat cecum; tissue adhesion