High-throughput transcriptome sequencing reveals the combined effects of key e-waste contaminants, decabromodiphenyl ether (BDE-209) and lead, in zebrafish larvae.
PBDEs and heavy metals are two major contaminants at e-waste disposal sites, but their combined effects remain largely unexplored. In the present study, the transcriptomic profiles of zebrafish larvae were examined after acute exposure of embryos to 200 μg/L BDE-209, 20 μg/L lead (Pb) or their mixture (Mix). Stimulation of steroidogenic pathway and vitellogenesis in the BDE-209 and Mix treatments indicated the estrogenic activities of BDE-209, while Pb antagonized those estrogenic effects in the Mix treatment. Increased heart rates were observed in zebrafish exposed to the Pb and Mix treatments. The cardiac dysfunction probably resulted from the promotion of angiogenesis, increased adrenergic drive and induction of the formation of blood clot. Furthermore, the Pb and Mix treatments activated neuroendocrine regulation of the pituitary in a positive feedback loop, via the thyrotropin-releasing hormone receptor, thus increasing thyroid hormone production self-adaptively. Overall, the interaction between BDE-209 and Pb led to synergistic and antagonistic effects on gene transcriptions, with concerted contribution from their individual toxicological properties.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, China.
Lianguo
L
Chen
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Biran
B
Zhu
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Yongyong
Y
Guo
Puai Hospital, Huazhong University of Science and Technology, Wuhan, 430033, China.
Tao
T
Xu
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, 16419, South Korea.
Jae-Seong
JS
Lee
Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, China.
Pei-Yuan
PY
Qian
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. Electronic address: bszhou@ihb.ac.cn.
Bingsheng
B
Zhou
D000818:Animals; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013961:Thyroid Gland / Q000378:metabolism; D059467:Transcriptome; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000494:pharmacology*; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
D055768:Halogenated Diphenyl Ethers; D062065:Wastewater; D014874:Water Pollutants, Chemical; C010902:decabromobiphenyl ether
BDE-209; Developmental co-exposure; Pb; Transcriptome; Zebrafish embryos