RIG-I ligand enhances the immunogenicity of recombinant H7HA protein.
Avian H7N9 influenza virus infection with fatal outcomes continues to pose a pandemic threat and highly immunogenic vaccines are urgently needed. In this report we show that baculovirus-derived recombinant H7 hemagglutinin protein, when delivered with RIG-I ligand, induced enhanced antibody and T cell responses and conferred protection against lethal challenge with a homologous H7N9 virus. These findings indicate the potential utility of RIG-I ligands as vaccine adjuvants to increase the immunogenicity of recombinant H7 hemagglutinin.
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA. Electronic address: wcao@cdc.gov.
Weiping
W
Cao
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
Justine S
JS
Liepkalns
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA; Battelle Memorial Institute, Atlanta, GA, USA.
Ram P
RP
Kamal
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
Adrian J
AJ
Reber
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
Jin Hyang
JH
Kim
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Amelia R
AR
Hofstetter
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA; Battelle Memorial Institute, Atlanta, GA, USA.
Samuel
S
Amoah
Virus, Surveillance and Diagnosis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
James
J
Stevens
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
Priya
P
Ranjan
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
Shivaprakash
S
Gangappa
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
Ian A
IA
York
Immunology and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, USA.
Suryaprakash
S
Sambhara
D000276:Adjuvants, Immunologic; D000818:Animals; D002478:Cells, Cultured; D000071457:DEAD Box Protein 58 / Q000276:immunology*; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D006801:Humans; D056724:Immunity, Humoral; D064766:Influenza A Virus, H7N9 Subtype / Q000276:immunology* / Q000378:metabolism; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D011971:Receptors, Immunologic; D051192:Receptors, Pattern Recognition / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D014614:Vaccines, Synthetic
D000276:Adjuvants, Immunologic; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011971:Receptors, Immunologic; D051192:Receptors, Pattern Recognition; D014614:Vaccines, Synthetic; C109963:hemagglutinin, avian influenza A virus; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58
Antibody responses; Cell-mediated immunity; H7HA; Influenza; Mouse model; Protective immunity; RIG-I ligand