Micropropagation and Biomass Production of True-to-Type Stevia rebaudiana Bertoni.
Here we describe an efficient micropropagation protocol for Stevia rebaudiana Bertoni. We present experiments carried out to optimize the suitable media for in vitro shoot multiplication and root induction and to study the effect of culture vessel on shoot multiplication. Among all different media tested for in vitro shoot multiplication, hormone-free liquid medium is most suitable. The highest number of nodes per shoot (5.4) and length of shoot (4.76 cm) at 4 weeks after subculturing are observed when single node explants are placed on modified MS medium supplemented with 1 % sucrose and 0.7 % agar. The highest response of multiplication rate (9.56) is observed on half strength of macroelement of MS with full strength of microelement of MS and 170 mg/l KH2PO4, and 185 mg/l MgSO4 in plastic growth container. Further, RAPD marker analysis of in vitro-raised plants maintained their clonal fidelity and true-to-type without showing any somaclonal variation.
Department of Agricultural Biotechnology, Plant Tissue Culture Lab, Anand Agricultural University, Anand, 388110, Gujarat, India.
Arpan R
AR
Modi
Department of Biotechnology, Arni University, Kathgarh, Indora, HP, India.
Vikas
V
Sharma
Department of Agricultural Biotechnology, Plant Tissue Culture Lab, Anand Agricultural University, Anand, 388110, Gujarat, India.
Ghanshyam
G
Patil
Department of Agricultural Biotechnology, Plant Tissue Culture Lab, Anand Agricultural University, Anand, 388110, Gujarat, India.
Amritpal S
AS
Singh
Department of Agricultural Biotechnology, Plant Tissue Culture Lab, Anand Agricultural University, Anand, 388110, Gujarat, India.
N
N
Subhash
Centre of Biological Sciences (Biotechnology), School of Earth, Biological and Environmental Science, Central University of Bihar, Patna, 800014, India. nitish@cub.ac.in.
Nitish
N
Kumar
D000362:Agar / Q000378:metabolism; D018533:Biomass; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D000069600:Plant Breeding / Q000379:methods; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D036625:Stevia / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D018744:DNA, Plant; D013395:Sucrose; D000362:Agar
Culture vessel; Genetic fidelity; Liquid culture; Micropropagation; RAPD; Stevia rebaudiana