Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses.
Bufavirus is a recently recognized member of the genus Protoparvovirus in the subfamily Parvovirinae. It has been reported that human bufavirus was detected predominantly in patients with diarrhoea in several countries. However, little is known about bufavirus or its close relatives in nonhuman mammals. In this study, we performed nested-PCR screening and identified bufavirus from 12 megabats of Pteropus spp. in Indonesia. Furthermore, we determined nearly the full genome sequence of a novel megabat-borne bufavirus, tentatively named megabat bufavirus 1. Phylogenetic analyses showed that megabat bufavirus 1 clustered with known protoparvoviruses, including human bufavirus but represented a distinct lineage of bufavirus. Our analyses also inferred phylogenetic relationships among animal-borne bufaviruses recently reported by other studies. Recombination analyses suggested that the most common recent ancestor of megabat bufavirus 1 might have arisen from multiple genetic recombination events. These results characterized megabat bufavirus 1 as the first protoparvovirus discovered from megabats and indicates the high genetic divergence of bufavirus.
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Michihito
M
Sasaki
Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Gabriel
G
Gonzalez
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Yuji
Y
Wada
Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
Agus
A
Setiyono
Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
Ekowati
E
Handharyani
Veterinary Investigation and Diagnostic Center, Bukittinggi, Indonesia.
Ibenu
I
Rahmadani
Faculty of Agriculture, Gorontalo State University, Gorontalo, Indonesia.
Siswatiana
S
Taha
Faculty of Animal Husbandry, Sam Ratulangi University, Manado, Indonesia.
Sri
S
Adiani
Office of Animal Husbandry and Fisheries, Soppeng, Indonesia.
Munira
M
Latief
Veterinary Medicine Study Program, Faculty of Medicine, Hasanudin University, Makassar, Indonesia.
Zainal Abidin
ZA
Kholilullah
Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
Mawar
M
Subangkit
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Shintaro
S
Kobayashi
Unit of International Cooperation, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, Japan.
Ichiro
I
Nakamura
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Takashi
T
Kimura
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Yasuko
Y
Orba
Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.|Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Hokkaido, Japan.
Kimihito
K
Ito
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.|Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Hokkaido, Japan.|Global Virus Network, Baltimore, MD, USA.
Hirofumi
H
Sawa
D000818:Animals; D036022:Capsid Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D002685:Chiroptera / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D010322:Parvoviridae Infections / Q000473:pathology / Q000662:veterinary / Q000821:virology; D017992:Parvovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; D004279:DNA, Viral