Autologous plasma and its supporting role in fat graft survival: A relevant vector to counteract resorption in lipofilling.
Fat grafting has become a widespread technique for different reconstructive and esthetic purposes. However, the disadvantage of fat grafting is the unpredictable resorption rate that often necessitates repetitive procedures, which in turn may have an impact on the morbidity. During the immediate, post-graft, ischemic period, cells survive due to the process of plasmatic imbibition. This biological phenomenon precedes the ingrowth of neo-capillaries that eventually nourish the graft and help establish a long-term homeostatic equilibrium. Both partners, the graft and the recipient bed, contribute to the revascularization process. Hypothetically, enrichment of the recipient site with autologous plasma could have a beneficial role to enhance fat graft survival. We investigated whether plasma supported the viability of the lipoaspirate (LA) material. Plasma was isolated from blood samples collected from eight patients during the elective lipofilling procedures. An in vitro study assessed the viability of LA cells using plasma as a culture medium compared to the traditional culture media. In vitro analysis confirmed sustained viability of LA cells compared to the standard media and control media during 7 consecutive days. The behavior of the fat grafts in plasma showed similarities with those incubated in the traditional culture media. In future, these findings could be translated to a clinical setting. Plasma is the only autologous substrate available in large quantities in the human body. The addition of the supporting agents, such as plasma, could contribute to a better graft survival with more stable clinical outcomes in the long term. The rationale behind the technique is based on the phenomenon of plasmatic imbibition and the reasoning that the extracellular matrix plays a pivotal role in cellular survival.
Department of Plastic and Reconstructive Surgery, Ghent University Hospital, Ghent, Belgium. Electronic address: filip.stillaert@ugent.be.
Filip
F
Stillaert
Department of Plastic and Reconstructive Surgery, Ghent University Hospital, Ghent, Belgium.
Bernard
B
Depypere
Department of Plastic and Reconstructive Surgery, Ghent University Hospital, Ghent, Belgium.
Maarten
M
Doornaert
Department of Pathology, Ghent University Hospital, Ghent, Belgium.
David
D
Creytens
Department of Basic Medical Science, Ghent University Hospital, Ghent, Belgium.
Heidi
H
De Clercq
Department of Basic Medical Science, Ghent University Hospital, Ghent, Belgium.
Ria
R
Cornelissen
Department of Plastic and Reconstructive Surgery, Ghent University Hospital, Ghent, Belgium.
Stan
S
Monstrey
Department of Plastic and Reconstructive Surgery, Ghent University Hospital, Ghent, Belgium.
Phillip
P
Blondeel
D000273:Adipose Tissue / Q000503:physiopathology / Q000637:transplantation; D001804:Blood Transfusion, Autologous; D018929:Cell Culture Techniques; D002470:Cell Survival; D006801:Humans; D066298:In Vitro Techniques; D015187:Lipectomy / Q000009:adverse effects* / Q000379:methods; D010949:Plasma / Q000502:physiology; D055031:Primary Graft Dysfunction / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control; D014182:Transplantation, Autologous; D019737:Transplants / Q000098:blood supply / Q000503:physiopathology
Fat graft; Lipofilling; Plasma; Plasmatic imbibition; Resorption