Association of Genetic Polymorphisms in TNF and MIF Gene with the Risk of Primary Dysmenorrhea.
Primary dysmenorrhea, which affects 90 % of adolescent girls and more than 50 % of menstruating women worldwide, is characterized by recurrent pain during menses in the absence of a detectable organic disease. The aim of this study is to assess the association between MIF -173 and TNF -308 genetic polymorphisms and the clinical features of primary dysmenorrhea. The study population comprised 154 unrelated female patients with clinical diagnosis of dysmenorrhea, and a total of 144 control subjects were recruited consecutively. The MIF -173G > C promoter polymorphism (rs755622) and TNF gene -308G > A (rs1800629) polymorphism were analyzed by polymerase chain reaction-based restriction fragment length polymorphism assay. Two fragments (268 and 97 bp) were seen when the G allele was present at position -173, and three fragments (206, 97, and 62 bp) were observed when the C allele was present. Two fragments (87 and 20 bp) were seen when G allele was present at position -308. There were statistically significant associations between age at menarche and history of back pain among dysmenorrhea patients and MIF gene -173G > C polymorphism (p = 0.003 and p = 0.042, respectively). The genotype and allele frequencies of -308G > A polymorphism showed statistically significant differences between dysmenorrhea patients and controls (p = 0.023 and p = 0.009, respectively). A high association was also observed when the patients were compared with the controls according to the GG genotype versus GA+AA genotypes (p = 0.009). The present study showed that the TNF-α -308 GG genotype may be a useful tool to predict the susceptibility of dysmenorrhea.
Department of Gynecology and Obstetrics, Medical Faculty, Gaziosmanpasa University, 60100, Tokat, Turkey. hatice_yilmaz47@hotmail.com.
Hatice Yilmaz
HY
Dogru
Department of Gynecology and Obstetrics, Medical Faculty, Gaziosmanpasa University, 60100, Tokat, Turkey.
Asker Zeki
AZ
Ozsoy
Department of Medical Biology, Medical Faculty, Gaziosmanpasa University, Tokat, Turkey.
Nevin
N
Karakus
Department of Gynecology and Obstetrics, Medical Faculty, Gaziosmanpasa University, 60100, Tokat, Turkey.
Ilhan Bahri
IB
Delibas
Department of Gynecology and Obstetrics, Medical Faculty, Gaziosmanpasa University, 60100, Tokat, Turkey.
Cigdem Kunt
CK
Isguder
Department of Medical Biology, Medical Faculty, Gaziosmanpasa University, Tokat, Turkey.
Serbulent
S
Yigit
D000328:Adult; D004412:Dysmenorrhea / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014421:Turkey; D055815:Young Adult
D008263:Macrophage Migration-Inhibitory Factors; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human
Dysmenorrhea; Macrophage migration inhibitory factors; Pain; Single nucleotide polymorphism; Tumor necrosis factor-alpha