Static magnetic field controls cell cycle in cultured human glioblastoma cells.
Magnetic field has been widely used in clinical diagnostics or for clinical treatment and is an important biomedical technology. Glioblastoma multiforme U87 and U251 are models of a fast growing malignant cancer. We focused on cellular level drafting of these cell lines as a time-dependent effect indicator of static magnetic fields (2000 ± 600 Gauss) by using their fast-growing properties. Cell viability showed a significant decrease (p < 0.01). The results coincided with the occurrence of apoptotic signals or protein expression of cyclin B1 and cyclin dependent kinase 1 in a non-apoptotic manner. Cdk1 was decreased in proportion to ankyrin G and cyclin B1 (Chi-square test, p = 0.0366). Our findings suggest that static magnetic stimulation creates a specific cyto-proliferative pattern, rather than producing randomized growth impairment.
Medical School, College of Medicine, Yonsei University, Yonsei-ro 50, Shinchon-dong, Seodaemoon-gu, Seoul, South Korea. synedit@gmail.com.
Seung Chan
SC
Kim
Seoul National University Hospital, Seoul, South Korea.
Wooseok
W
Im
Seoul National University Hospital, Seoul, South Korea.
Jay Yong
JY
Shim
Seoul National University Hospital, Seoul, South Korea.
Seung-Ki
SK
Kim
Department of Mathematics, Yonsei University, Seoul, South Korea.
Beom Jin
BJ
Kim
Cell cycle; Glioblastoma; Specified pattern; Static magnetic field; Viability