Autophagy activity is associated with membranous sodium iodide symporter expression and clinical response to radioiodine therapy in non-medullary thyroid cancer.
Although non-medullary thyroid cancer (NMTC) generally has a good prognosis, 30-40% of patients with distant metastases develop resistance to radioactive iodine (RAI) therapy due to tumor dedifferentiation. For these patients, treatment options are limited and prognosis is poor. In the present study, expression and activity of autophagy was assessed in large sets of normal, benign and malignant tissues and was correlated with pathology, SLC5A5/hNIS (solute carrier family 5 member 5) protein expression, and with clinical response to RAI ablation therapy in NMTC patients. Fluorescent immunostaining for the autophagy marker LC3 was performed on 100 benign and 80 malignant thyroid tissues. Semiquantitative scoring was generated for both diffuse LC3-I intensity and number of LC3-II-positive puncta and was correlated with SLC5A5 protein expression and clinical parameters. Degree of diffuse LC3-I intensity and number of LC3-II-positive puncta scoring were not discriminative for benign vs. malignant thyroid lesions. Interestingly, however, in NMTC patients significant associations were observed between diffuse LC3-I intensity and LC3-II-positive puncta scoring on the one hand and clinical response to RAI therapy on the other hand (odds ratio [OR] = 3.13, 95% confidence interval [CI] =1.91-5.12, P = 0.01; OR = 5.68, 95%CI = 3.02-10.05, P = 0.002, respectively). Mechanistically, the number of LC3-II-positive puncta correlated with membranous SLC5A5 expression (OR = 7.71, 95%CI = 4.15-11.75, P<0.001), number of RAI treatments required to reach remission (P = 0.014), cumulative RAI dose (P = 0.026) and with overall remission and recurrence rates (P = 0.031). In conclusion, autophagy activity strongly correlates with clinical response of NMTC patients to RAI therapy, potentially by its capacity to maintain tumor cell differentiation and to preserve functional iodide uptake.
a Department of Internal Medicine , Radboud University Medical Center , Nijmegen , The Netherlands.|b Division of Endocrinology , Radboud University Medical Center , Nijmegen , The Netherlands.|c Department of Pathology , Radboud University Medical Center , Nijmegen , The Netherlands.
Theo S
TS
Plantinga
a Department of Internal Medicine , Radboud University Medical Center , Nijmegen , The Netherlands.|b Division of Endocrinology , Radboud University Medical Center , Nijmegen , The Netherlands.|c Department of Pathology , Radboud University Medical Center , Nijmegen , The Netherlands.
Marika H
MH
Tesselaar
d Department of Pathology , Leiden University Medical Center , Leiden , The Netherlands.
Hans
H
Morreau
e Department of Endocrinology and Metabolic Diseases , Leiden University Medical Center , Leiden , The Netherlands.
Eleonora P M
EP
Corssmit
c Department of Pathology , Radboud University Medical Center , Nijmegen , The Netherlands.
Brigith K
BK
Willemsen
c Department of Pathology , Radboud University Medical Center , Nijmegen , The Netherlands.
Benno
B
Kusters
c Department of Pathology , Radboud University Medical Center , Nijmegen , The Netherlands.
A C H
AC
van Engen-van Grunsven
a Department of Internal Medicine , Radboud University Medical Center , Nijmegen , The Netherlands.|b Division of Endocrinology , Radboud University Medical Center , Nijmegen , The Netherlands.
Johannes W A
JW
Smit
a Department of Internal Medicine , Radboud University Medical Center , Nijmegen , The Netherlands.|b Division of Endocrinology , Radboud University Medical Center , Nijmegen , The Netherlands.
Romana T
RT
Netea-Maier
D001343:Autophagy / Q000187:drug effects*; D002291:Carcinoma, Papillary / Q000175:diagnosis* / Q000188:drug therapy*; D006801:Humans; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D011379:Prognosis; D027981:Symporters / Q000378:metabolism*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000188:drug therapy*; D000099209:Sodium-Iodide Symporters
D007457:Iodine Radioisotopes; D027981:Symporters; D000099209:Sodium-Iodide Symporters
LC3; autophagy; puncta; radioactive iodine therapy; thyroid carcinoma