Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.
Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.|The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
Fuqiang
http://orcid.org/0000-0003-1447-9916
F
Huang
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, China.
Zhisheng
Z
Dang
Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan.
Yutaka
Y
Suzuki
Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan.
Terumi
T
Horiuchi
Department of Infectious Disease, Hokkaido Institute of Public Health, Sapporo, Hokkaido, Japan.
Kinpei
K
Yagi
Department of Infectious Disease, Hokkaido Institute of Public Health, Sapporo, Hokkaido, Japan.
Hirokazu
H
Kouguchi
Department of Infectious Disease, Hokkaido Institute of Public Health, Sapporo, Hokkaido, Japan.
Takao
T
Irie
Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
Kyeongsoon
K
Kim
Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
Yuzaburo
Y
Oku
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis; D048210:Echinococcus multilocularis / Q000235:genetics* / Q000254:growth & development*; D059014:High-Throughput Nucleotide Sequencing; D059467:Transcriptome
D000947:Antigens, Helminth