Development of marker genes for jasmonic acid signaling in shoots and roots of wheat.
The jasmonic acid (JA) signaling pathway plays key roles in a diverse array of plant development, reproduction, and responses to biotic and abiotic stresses. Most of our understanding of the JA signaling pathway derives from the dicot model plant Arabidopsis thaliana, while corresponding knowledge in wheat is somewhat limited. In this study, the expression of 41 genes implicated in the JA signaling pathway has been assessed on 10 day-old bread wheat seedlings, 24 h, 48 h, and 72 h after methyl-jasmonate (MeJA) treatment using quantitative real-time PCR. The examined genes have been previously reported to be involved in JA biosynthesis and catabolism, JA perception and signaling, and pathogen defense in wheat shoots and roots. This study provides evidence to suggest that the effect of MeJA treatment is more prominent in shoots than roots of wheat seedlings, and substantial regulation of the JA pathway-dependent defense genes occurs at 72 h after MeJA treatment. Results show that the expression of 22 genes was significantly affected by MeJA treatment in wheat shoots. However, only PR1.1 and PR3 were significantly differentially expressed in wheat roots, both at 24 h post-MeJA treatment, with other genes showing large variation in their gene expression in roots. While providing marker genes on JA signaling in wheat, future work may focus on elucidating the regulatory function of JA-modulated transcription factors, some of which have well-studied potential orthologs in Arabidopsis.
a Plant-Microbe Interactions Laboratory, School of Agriculture and Food Sciences, The University of Queensland , Brisbane , Queensland , Australia.
Hongwei
H
Liu
a Plant-Microbe Interactions Laboratory, School of Agriculture and Food Sciences, The University of Queensland , Brisbane , Queensland , Australia.
Lilia Costa
LC
Carvalhais
b Commonwealth Scientific and Industrial Research Organization (CSIRO) Plant Industry, Queensland Bioscience Precinct , Brisbane , Queensland , Australia.
Kemal
K
Kazan
a Plant-Microbe Interactions Laboratory, School of Agriculture and Food Sciences, The University of Queensland , Brisbane , Queensland , Australia.
Peer M
PM
Schenk
D000085:Acetates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005819:Genetic Markers; D054883:Oxylipins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000276:immunology
D000085:Acetates; D003517:Cyclopentanes; D005819:Genetic Markers; D054883:Oxylipins; C011006:jasmonic acid; C072239:methyl jasmonate
Jasmonate; PR genes; marker genes; transcription factor; wheat