Super-resolved insights into human immunodeficiency virus biology.
The recent development of fluorescence microscopy approaches overcoming the diffraction limit of light microscopy opened possibilities for studying small-scale cellular processes. The spatial resolution achieved by these novel techniques, together with the possibility to perform live-cell and multicolor imaging, make them ideally suited for visualization of native viruses and subviral structures within the complex environment of a host cell or organ, thus providing fundamentally new possibilities for investigating virus-cell interactions. Here, we review the use of super-resolution microscopy approaches to study virus-cell interactions, and discuss recent insights into human immunodeficiency virus biology obtained by exploiting these novel techniques.
Department of Infectious Diseases, Virology, University Hospital Heidelberg, Germany.|Optical Nanoscopy Division, German Cancer Research Center, Heidelberg, Germany.
Janina
J
Hanne
Department of Infectious Diseases, Virology, University Hospital Heidelberg, Germany.
Vojtech
V
Zila
Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Germany.
Mike
M
Heilemann
Department of Infectious Diseases, Virology, University Hospital Heidelberg, Germany.
Barbara
B
Müller
Department of Infectious Diseases, Virology, University Hospital Heidelberg, Germany.
Hans-Georg
HG
Kräusslich
D006678:HIV / Q000502:physiology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence / Q000379:methods*; D014779:Virus Replication
correlative microscopy; fluorescence; human immunodeficiency virus; super-resolution microscopy