Labeling of target mRNAs using a photo-reactive microRNA probe.
To identify target mRNAs of an miRNA, we synthesized photo-reactive miRNA probes, which contained a photo-reactive nucleoside analog, 1-O-[4-(3-trifluoromethyl-3H-diazirine-3-yl)]benzyl-β-d-ribofuranose, in the middle of the strand. The photo-reactive miRNA-145 probe was found to specifically label the target mRNAs, FSCN1 and KLF4, by UV-A irradiation in human colon cancer DLD-1 cells.
United Graduate School of Agricultural Science, Gifu University, Japan. uenoy@gifu-u.ac.jp.
Kosuke
K
Nakamoto
United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan.
Koichiro
K
Minami
United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan.
Yukihiro
Y
Akao
United Graduate School of Agricultural Science, Gifu University, Japan. uenoy@gifu-u.ac.jp and Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Yoshihito
Y
Ueno
D002352:Carrier Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D045744:Cell Line, Tumor; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000037:antagonists & inhibitors* / Q000235:genetics; D035683:MicroRNAs / Q000737:chemistry / Q000494:pharmacology*; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D015335:Molecular Probes / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000235:genetics; D014466:Ultraviolet Rays
D002352:Carrier Proteins; C502658:FSCN1 protein, human; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; D008840:Microfilament Proteins; D015335:Molecular Probes; D012333:RNA, Messenger