Influence of Butyrate Loaded Clinoptilolite Dietary Supplementation on Growth Performance, Development of Intestine and Antioxidant Capacity in Broiler Chickens.
The study was conducted to evaluate the effects of dietary butyrate loaded clinoptilolite (CLI-B) on growth performance, pancreatic digestive enzymes, intestinal development and histomorphology, as well as antioxidant capacity of serum and intestinal mucosal in chickens. Two hundred forty 1-day-old commercial Arbor Acres broilers were randomly assigned to 4 groups: CON group (fed basal diets), SB group (fed basal diet with 0.05% sodium butyrate), CLI group (fed basal diet with 1% clinoptilolite), and CLI-B group (fed basal diet with 1% CLI-B). The results showed that supplementation of CLI-B significantly decreased (P < 0.05) feed conservation ratio at both 21 and 42 days of age, improved the pancreatic digestive enzymes activities (P < 0.05), increased the villus length and villus/crypt ratio (P < 0.05), and decreased the crypt depth of intestine (P < 0.05) as compared to the other experimental groups. Furthermore, the CLI-B environment improved the antioxidant capacity by increasing the antioxidant enzyme activities (P < 0.05) in intestine mucosal, and decreasing the NO content and iNOS activity (P < 0.05) in serum. In addition, CLI-B supplementation had improved the development of intestine and antioxidant capacity of broilers than supplementation with either clinoptilolite or butyrate sodium alone. In conclusion, 1% CLI-B supplementation improved the health status, intestine development and antioxidant capacity in broiler chickens, thus appearing as an important feed additive for the poultry industry.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Yanan
Y
Wu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Yanmin
Y
Zhou
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Changhui
C
Lu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Hussain
H
Ahmad
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Hao
H
Zhang
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Jintian
J
He
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Lili
L
Zhang
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Tian
T
Wang
D000681:Amylases / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D020148:Butyric Acid / Q000008:administration & dosage*; D002374:Catalase / Q000378:metabolism; D002645:Chickens / Q000254:growth & development* / Q000378:metabolism; D019587:Dietary Supplements; D004063:Digestion / Q000187:drug effects; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D008460:Meat; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017641:Zeolites / Q000008:administration & dosage*
D000975:Antioxidants; D020148:Butyric Acid; C083175:clinoptilolite; D017641:Zeolites; D009569:Nitric Oxide; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases