Loss of expression of BAP1 is very rare in non-small cell lung carcinoma.
Germline mutations of the BAP1 gene have been implicated in a cancer predisposition syndrome which includes mesothelioma, uveal melanoma, cutaneous melanocytic lesions, renal cell carcinoma, and possibly other malignancies. Double hit inactivation of BAP1 with subsequent loss of expression of the BAP1 protein also occurs in approximately 50% of mesotheliomas. The link between BAP1 mutation and lung cancer is yet to be fully explored. We sought to assess BAP1 expression in a large cohort of lung cancers undergoing surgery with curative intent. We searched the Anatomical Pathology database of our institution for lung cancer patients undergoing surgery with curative intent between 2000 and 2010. Immunohistochemistry for BAP1 was then performed in tissue microarray format. Our cohort included 257 lung cancer patients, of which 155 (60%) were adenocarcinomas and 72 (28%) were squamous cell carcinomas, with no other subtype comprising more than 3%. BAP1 loss of expression was found in only one lung cancer. We conclude that BAP1 mutation occurs very infrequently (0.4%) in non-small cell lung cancer. Given that the pathological differential diagnosis between lung carcinoma and mesothelioma may sometimes be difficult, this finding increases the specificity of loss of expression for BAP1 for the diagnosis of mesothelioma.
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia; Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia.
Juliana
J
Andrici
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia.
Thomas R
TR
Parkhill
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia.
Jason
J
Jung
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia.
Kathryn L
KL
Wardell
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia.
Brandon
B
Verdonk
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia.
Arjun
A
Singh
Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia.
Loretta
L
Sioson
Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia; Department of Anatomical Pathology, Royal North Shore Hospital, St Leonards, NSW, Australia.
Adele
A
Clarkson
Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia.
Nicole
N
Watson
Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia.
Amy
A
Sheen
Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia.
Mahtab
M
Farzin
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia; Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia; Histopath Pathology, North Ryde, St Leonards, NSW, Australia.
Christopher W
CW
Toon
Sydney Medical School, University of Sydney, St Leonards, NSW, Australia; Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, St Leonards, NSW, Australia; Department of Anatomical Pathology, Royal North Shore Hospital, St Leonards, NSW, Australia; Sydney Vital Translational Research Centre, Royal North Shore Hospital, St Leonards, NSW, Australia. Electronic address: affgill@med.usyd.edu.au.
Anthony J
AJ
Gill
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016208:Databases, Factual; D005260:Female; D020022:Genetic Predisposition to Disease; D018095:Germ-Line Mutation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000235:genetics* / Q000378:metabolism
C400134:BAP1 protein, human; D025521:Tumor Suppressor Proteins; D043222:Ubiquitin Thiolesterase
BAP1; BRCA1 associated protein 1; Lung cancer