Association of Interleukin-1B and Interleukin-4 Gene Variants with Autoimmune Thyroid Diseases in Tunisian Population.
Autoimmune thyroid diseases (AITD) including Graves' disease (GD) and Hashimoto's thyroiditis (HT) are complex genetic diseases. Cytokines IL-1B and IL-4 play a role in the pathogenesis of AITD. This study was conducted on Tunisian patients with GD or HT to investigate the association of IL-1B and IL-4 gene polymorphisms with the risk and the prognosis of AITD. A total of 358 healthy controls and 341 patients with AITDs (249 HT and 92 GD) were genotyped for IL-1B+3953C/T and IL-4 intron 3 VNTR polymorphisms. A significant association was found between IL-1B+3953C/T polymorphism and GD or HT, both in the dominant and additive models. The IL-1B+3953T allele was associated with GD (p = 0.0003, OR = 1.93, CI = 1.34-2.78) and HT (p = 0.009, OR = 1.43, CI = 1.09-1.88). The IL-4 VNTR polymorphism was associated only with HT risk both in additive (p = 0.03, OR = 0.31, CI = 0.11-0.86) and recessive (p = 0.03, OR = 3.04, CI = 1.13-8.17) models. No significant association was found between IL-1B+3953C/T polymorphism and change in the serum concentrations of TSH and FT4 in GD and HT patients. In HT patients, the IL-1B+3953T allele (p = 0.009, OR = 0.42, CI = 0.22-0.83) and the IL-1B+3953T/T genotype (p = 0.03, OR = 0.21, CI = 0.04-1.07) were more frequent in the absence than in the presence of an anti-TPO antibody. The proportion of HT patients with the P1P2 genotype of the IL-4 gene was significantly higher in the absence than in the presence of the anti-TPO antibody (p = 0.04, OR = 0.39, CI = 0.17-0.89). These preliminary results suggest that IL-1B and IL-4 gene polymorphisms may be associated with GD and HT susceptibility and may represent prognostic factors for predicting the severity of HT.
a Laboratory of Genetics, Biodiversity and Bioresource Valorization , Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
Ines
I
Zaaber
a Laboratory of Genetics, Biodiversity and Bioresource Valorization , Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
Souhir
S
Mestiri
a Laboratory of Genetics, Biodiversity and Bioresource Valorization , Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
Hounayda
H
Hammedi
b Department of Internal Medicine-Endocrinology , Hospital Fattouma Bourguiba in Monastir , Monastir , Tunisia.
Hela
H
Marmouch
b Department of Internal Medicine-Endocrinology , Hospital Fattouma Bourguiba in Monastir , Monastir , Tunisia.
Silvia
S
Mahjoub
a Laboratory of Genetics, Biodiversity and Bioresource Valorization , Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
Besma Bel Hadj Jrad
BB
Tensaout
a Laboratory of Genetics, Biodiversity and Bioresource Valorization , Superior Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia.
Khaled
K
Said
D000328:Adult; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D011110:Polymorphism, Genetic; D012306:Risk; D013967:Thyroiditis, Autoimmune / Q000235:genetics*; D013972:Thyrotropin / Q000097:blood; D014416:Tunisia
C508598:IL4 protein, human; D053583:Interleukin-1beta; D015847:Interleukin-4; D013972:Thyrotropin
AITD, anti-thyroglobulin antibody; IL-1B; IL-4; anti-thyroid peroxidase antibody