Proteomic Analysis of Stage-II Breast Cancer from Formalin-Fixed Paraffin-Embedded Tissues.
Breast cancer is the most frequently occurring disease among women worldwide. The early stage of breast cancer identification is the key challenge in cancer control and prevention procedures. Although gene expression profiling helps to understand the molecular mechanism of diseases or disorder in the living system, gene expression pattern alone is not sufficient to predict the exact mechanisms. Current proteomics tools hold great application for analysis of cancerous conditions. Hence, the generation of differential protein expression profiles has been optimized for breast cancer and normal tissue samples in our organization. Normal and tumor tissues were collected from 20 people from a local hospital. Proteins from the diseased and normal tissues have been investigated by 2D gel electrophoresis and MALDI-TOF-MS. The peptide mass fingerprint data were fed into various public domains like Mascot, MS-Fit, and Pept-ident against Swiss-Prot protein database and the proteins of interest were identified. Some of the differentially expressed proteins identified were human annexin, glutathione S-transferase, vimentin, enolase-1, dihydrolipoamide dehydrogenase, glutamate dehydrogenase, Cyclin A1, hormone sensitive lipase, beta catenin, and so forth. Many types of proteins were identified as fundamental steps for developing molecular markers for diagnosis of human breast cancer as well as making a new proteomic database for future research.
Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Naif
N
Abdullah Al-Dhabi
Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-eup, Cheonan-si, Chungnam 330801, Republic of Korea.
Srisesharam
S
Srigopalram
Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-eup, Cheonan-si, Chungnam 330801, Republic of Korea.
Soundharrajan
S
Ilavenil
Department of Medicinal Crop Research, Rural Development Administration, Eumseong, Chungbuk 369-873, Republic of Korea.
Young Ock
YO
Kim
Research Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai, Tamil Nadu 600034, India.
Paul
P
Agastian
Proteomics Division, Discovery Research, Dr. Reddy's Laboratories Ltd., Miyapur, Hyderabad 500049, India.
Rajasekhar
R
Baaru
Sharmila Institute of Medicinal Products Research Academy, Thanjavur 613007, India.
Kannan
K
Balamurugan
Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-eup, Cheonan-si, Chungnam 330801, Republic of Korea.
Ki Choon
KC
Choi
Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Mariadhas
M
Valan Arasu
D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005557:Formaldehyde; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010232:Paraffin; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D005557:Formaldehyde; D010232:Paraffin