Tau mutant A152T, a risk factor for FTD/PSP, induces neuronal dysfunction and reduced lifespan independently of aggregation in a C. elegans Tauopathy model.
BACKGROUND
A certain number of mutations in the Microtubule-Associated Protein Tau (MAPT) gene have been identified in individuals with high risk to develop neurodegenerative diseases, collectively called tauopathies. The mutation A152TMAPT was recently identified in patients diagnosed with frontotemporal spectrum disorders, including Progressive Supranuclear Palsy (PSP), Frontotemporal Dementia (FTD), Corticobasal Degeneration (CBD), and Alzheimer disease (AD). The A152TMAPT mutation is unusual since it lies within the N-terminal region of Tau protein, far outside the repeat domain that is responsible for physiological Tau-microtubule interactions and pathological Tau aggregation. How A152TMAPT causes neurodegeneration remains elusive.


RESULTS
To understand the pathological consequences of this mutation, here we present a new Caenorhabditis elegans model expressing the mutant A152TMAPT in neurons. While expression of full-length wild-type human tau (Tau(wt), 2N4R) in C. elegans neurons induces a progressive mild uncoordinated locomotion in a dose-dependent manner, mutant tau (Tau(A152T), 2N4R) induces a severe paralysis accompanied by acute neuronal dysfunction. Mutant Tau(A152T) worms display morphological changes in neurons reminiscent of neuronal aging and a shortened life-span. Moreover, mutant A152T overexpressing neurons show mislocalization of pre-synaptic proteins as well as distorted mitochondrial distribution and trafficking. Strikingly, mutant tau-transgenic worms do not accumulate insoluble tau aggregates, although soluble oligomeric tau was detected. In addition, the full-length A152T-tau remains in a pathological conformation that accounts for its toxicity. Moreover, the N-terminal region of tau is not toxic per se, despite the fact that it harbours the A152T mutation, but requires the C-terminal region including the repeat domain to move into the neuronal processes in order to execute the pathology.


CONCLUSIONS
In summary, we show that the mutant Tau(A152T) induces neuronal dysfunction, morphological alterations in neurons akin to aging phenotype and reduced life-span independently of aggregation. This comprehensive description of the pathology due to Tau(A152T) opens up multiple possibilities to identify cellular targets involved in the Tau-dependent pathology for a potential therapeutic intervention.
German Center for Neurodegenerative Diseases (DZNE), Ludwig-Erhard-Allee 2, 53175, Bonn, Germany. Jeelani.Pir@dzne.de.|Max-Planck-Institute for Metabolism Research (Cologne), Hamburg Outstation, c/o DESY, Notkestrasse 85, 22607, Hamburg, Germany. Jeelani.Pir@dzne.de.
Ghulam Jeelani
GJ
Pir
German Center for Neurodegenerative Diseases (DZNE), Ludwig-Erhard-Allee 2, 53175, Bonn, Germany.|Max-Planck-Institute for Metabolism Research (Cologne), Hamburg Outstation, c/o DESY, Notkestrasse 85, 22607, Hamburg, Germany.
Bikash
B
Choudhary
German Center for Neurodegenerative Diseases (DZNE), Ludwig-Erhard-Allee 2, 53175, Bonn, Germany.|Caesar Research Center, Ludwig-Erhard-Allee 2, 53175, Bonn, Germany.|Max-Planck-Institute for Metabolism Research (Cologne), Hamburg Outstation, c/o DESY, Notkestrasse 85, 22607, Hamburg, Germany.
Eckhard
E
Mandelkow
German Center for Neurodegenerative Diseases (DZNE), Ludwig-Erhard-Allee 2, 53175, Bonn, Germany. eva.mandelkow@dzne.de.|Caesar Research Center, Ludwig-Erhard-Allee 2, 53175, Bonn, Germany. eva.mandelkow@dzne.de.|Max-Planck-Institute for Metabolism Research (Cologne), Hamburg Outstation, c/o DESY, Notkestrasse 85, 22607, Hamburg, Germany. eva.mandelkow@dzne.de.
Eva-Maria
EM
Mandelkow
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans; D006801:Humans; D009154:Mutation / Q000235:genetics*; D009474:Neurons / Q000378:metabolism*; D010641:Phenotype; D012307:Risk Factors; D016875:tau Proteins / Q000235:genetics*
C054369:MAPT protein, human; D016875:tau Proteins
AD, Alzheimer disease; CBD, Corticobasal degeneration; FTD, Frontotemporal dementia; PSP, Progressive supranuclear palsy