In Vitro Regeneration of Endangered Medicinal Plant Heliotropium kotschyi (Ramram).
Heliotropium kotschyi (Ramram) is an important endangered medicinal plant distributed in the Kingdom of Bahrain. Plant tissue culture technique is applied for ex situ conservation study. Nodal stem segments are cultured in modified MS media supplemented with various combination and concentration of plant growth regulators (PGRs). Plants are regenerated via shoot organogenesis from the nodal meristems. Plants are regenerated in three different steps: initial shoot development, shoot multiplication, and rooting. After 4 weeks of culture, 100 % explants respond to shoot initiation on the medium containing 8.88 μM BAP and 5.71 μM IAA. The highest frequency of shoot regeneration is observed in the same media after second subculture of shoots. The highest rooting frequency is observed in the presence of 2.85 μM IAA. After root development, the plantlets are transferred to pots filled with soil and 60 % of plants survived after 45 days. This plant regeneration protocol is of great value for rapid desert plant propagation program.
Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain.
Manal Ahmed
MA
Sadeq
Department of Life Sciences, Agricultural Biotechnology Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain. malabikar@yahoo.com.
Malabika Roy
MR
Pathak
Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain.
Ahmed Ali
AA
Salih
Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain.
Mohammed
M
Abido
Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain.
Asma
A
Abahussain
D001593:Benzyl Compounds / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D056727:Endangered Species; D028162:Heliotropium / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000502:physiology; D018520:Plant Shoots / Q000502:physiology; D010946:Plants, Medicinal / Q000502:physiology*; D011687:Purines / Q000378:metabolism; D012038:Regeneration; D046509:Tissue Culture Techniques / Q000379:methods
D001593:Benzyl Compounds; D003470:Culture Media; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; C030737:indoleacetic acid; C480551:benzylaminopurine
Endangered; Ex situ conservation; Heliotropium kotschyi; Organogenesis; Plant regeneration