Albuminated Glycoenzymes: Enzyme Stabilization through Orthogonal Attachment of a Single-Layered Protein Shell around a Central Glycoenzyme Core.
Here we demonstrate an approach to stabilize enzymes through the orthogonal covalent attachment of albumin on the single-enzyme level. Albuminated glycoenzymes (AGs) based upon glucose oxidase and catalase from Aspergillus niger were prepared in this manner. Gel filtration chromatography and dynamic light scattering support modification, with an increase in hydrodynamic radius of ca. 60% upon albumination. Both AGs demonstrate a marked resistance to aggregation during heating to 90 °C, but this effect is more profound in albuminated catalase. The functional characteristics of albuminated glucose oxidase vary considerably with exposure type. The AG's thermal inactivation is reduced more than 25 times compared to native glucose oxidase, and moderate stabilization is observed with one month storage at 37 °C. However, albumination has no effect on operational stability of glucose oxidase.
Department of Biomedical Engineering, Texas A&M University , College Station, Texas 77843-3120, United States.
Dustin W
DW
Ritter
Department of Biomedical Engineering, Texas A&M University , College Station, Texas 77843-3120, United States.
Jared M
JM
Newton
Department of Biomedical Engineering, Texas A&M University , College Station, Texas 77843-3120, United States.
Jason R
JR
Roberts
Department of Biomedical Engineering, Texas A&M University , College Station, Texas 77843-3120, United States.|Department of Materials Science & Engineering, Texas A&M University , College Station, Texas 77843-3003, United States.
Michael J
MJ
McShane
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D002374:Catalase / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D004795:Enzyme Stability; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013696:Temperature
D012710:Serum Albumin, Bovine; D005949:Glucose Oxidase; D002374:Catalase