Gut commensal Bacteroides acidifaciens prevents obesity and improves insulin sensitivity in mice.
In humans, the composition of gut commensal bacteria is closely correlated with obesity. The bacteria modulate metabolites and influence host immunity. In this study, we attempted to determine whether there is a direct correlation between specific commensal bacteria and host metabolism. As mice aged, we found significantly reduced body weight and fat mass in Atg7ΔCD11cmice when compared with Atg7f/fmice. When mice shared commensal bacteria by co-housing or feces transfer experiments, body weight and fat mass were similar in both mouse groups. By pyrosequencing analysis, Bacteroides acidifaciens (BA) was significantly increased in feces of Atg7ΔCD11cmice compared with those of control Atg7f/fmice. Wild-type C57BL/6 (B6) mice fed with BA were significantly more likely to gain less weight and fat mass than mice fed with PBS. Of note, the expression level of peroxisome proliferator-activated receptor alpha (PPARα) was consistently increased in the adipose tissues of Atg7ΔCD11cmice, B6 mice transferred with fecal microbiota of Atg7ΔCD11cmice, and BA-fed B6 mice. Furthermore, B6 mice fed with BA showed elevated insulin levels in serum, accompanied by increased serum glucagon-like peptide-1 and decreased intestinal dipeptidyl peptidase-4. These finding suggest that BA may have potential for treatment of metabolic diseases such as diabetes and obesity.
Mucosal Immunology Laboratory, Department of Convergence Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Korea.|Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
J-Y
JY
Yang
Mucosal Immunology Laboratory, Department of Convergence Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Korea.
Y-S
YS
Lee
Mucosal Immunology Laboratory, Department of Convergence Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Korea.
Y
Y
Kim
Mucosal Immunology Laboratory, Department of Convergence Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Korea.
S-H
SH
Lee
Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
S
S
Ryu
Institute for Advanced Biosciences, Keio University, Yamagata, Japan.
S
S
Fukuda
Department of Biochemistry, Graduate School of Pharmacological Science, Keio University, Tokyo, Japan.
K
K
Hase
Department of Molecular and Life Science, College of Science and Technology, Hanyang University, Ansan, Korea.
C-S
CS
Yang
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Korea.
H S
HS
Lim
Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Korea.
M-S
MS
Kim
Department of Internal Medicine, Yonsei University, Wonju College of Medicine, Wonju, Korea.
H-M
HM
Kim
Laboratory of Pharmaceutics, College of Pharmacy, Kangwon National University, Chuncheon, Korea.
S-H
SH
Ahn
Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, Korea.
B-E
BE
Kwon
Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, Korea.
H-J
HJ
Ko
Mucosal Immunology Laboratory, Department of Convergence Medicine, University of Ulsan College of Medicine/Asan Medical Center, Seoul, Korea.
M-N
MN
Kweon
D000273:Adipose Tissue / Q000382:microbiology / Q000502:physiology*; D000818:Animals; D000071193:Autophagy-Related Protein 7 / Q000235:genetics; D001439:Bacteroides / Q000276:immunology*; D002478:Cells, Cultured; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000276:immunology*; D005786:Gene Expression Regulation; D052216:Glucagon-Like Peptide 1 / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000276:immunology*; D007422:Intestines / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009765:Obesity / Q000276:immunology / Q000382:microbiology*; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis
C502661:Atg7 protein, mouse; D007328:Insulin; D047493:PPAR alpha; D052216:Glucagon-Like Peptide 1; D018819:Dipeptidyl Peptidase 4; D000071193:Autophagy-Related Protein 7