Effects of Non-Collagenous Proteins, TGF-β1, and PDGF-BB on Viability and Proliferation of Dental Pulp Stem Cells.
OBJECTIVE
The dentin matrix servers as a reservoir of growth factors, sequestered during dentinogenesis. The aim of this study was to assess the viability and proliferation of dental pulp stem cells in the presence of dentin matrix-derived non-collagenous proteins and two growth factors; platelet-derived growth factor BB and transforming growth factor beta 1.


METHODS
The dental pulp cells were isolated and cultured. The dentin proteins were extracted and purified. The MTT assay was performed for assessment of cell viability and proliferation in the presence of different concentrations of dentin proteins and growth factors during 24 - 72 h post-treatment.


RESULTS
The cells treated with 250 ng/mL dentin proteins had the best viability and proliferation ability in comparison with other concentrations (P < 0.05). The MTT assay demonstrated that cells cultured with 5 ng/mL platelet-derived growth factor BB had the highest viability at each time point as compared to other groups (P < 0.05). However, in presence of platelet-derived growth factor BB alone and in combination with transforming growth factor beta 1 and dentin proteins (10 ng/mL), significant higher viability was seen at all time points (P < 0.05). The least viability and proliferation at each growth factor concentration was seen in cells treated with combination of transforming growth factor beta 1 and dentin proteins at 72 h (P < 0.05).


CONCLUSIONS
The results indicated that the triple combination of growth factors and matrix-derived non-collagenous proteins (especially at 10 ng/mL concentration) has mitogenic effect on dental pulp stem cells.
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical sciencesIran.; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesIran.
Fahimeh Sadat
FS
Tabatabaei
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical sciencesIran.; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesIran.
Maryam
M
Torshabi
dental pulp; dentin protein (non-collagenous); platelet-derived growth factor bb; transforming growth factor beta