A Cold-Inducible DEAD-Box RNA Helicase from Arabidopsis thaliana Regulates Plant Growth and Development under Low Temperature.
DEAD-box RNA helicases comprise a large family and are involved in a range of RNA processing events. Here, we identified one of the Arabidopsis thaliana DEAD-box RNA helicases, AtRH7, as an interactor of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3), which is an RNA chaperone involved in cold adaptation. Promoter:GUS transgenic plants revealed that AtRH7 is expressed ubiquitously and that its levels of the expression are higher in rapidly growing tissues. Knockout mutant lines displayed several morphological alterations such as disturbed vein pattern, pointed first true leaves, and short roots, which resemble ribosome-related mutants of Arabidopsis. In addition, aberrant floral development was also observed in rh7 mutants. When the mutants were germinated at low temperature (12°C), both radicle and first leaf emergence were severely delayed; after exposure of seedlings to a long period of cold, the mutants developed aberrant, fewer, and smaller leaves. RNA blots and circular RT-PCR revealed that 35S and 18S rRNA precursors accumulated to higher levels in the mutants than in WT under both normal and cold conditions, suggesting the mutants are partially impaired in pre-rRNA processing. Taken together, the results suggest that AtRH7 affects rRNA biogenesis and plays an important role in plant growth under cold.
Hokkaido Agriculture Research Center, National Agriculture and Food Research Organization, Sapporo, Japan.|Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Yuelin
Y
Liu
Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Daisuke
D
Tabata
Hokkaido Agriculture Research Center, National Agriculture and Food Research Organization, Sapporo, Japan.|Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Ryozo
R
Imai
D000064:Acclimatization; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003080:Cold Temperature; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D018525:Germination; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D060066:Protein Interaction Maps; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; C544303:CSP3 protein, Arabidopsis; D006360:Heat-Shock Proteins; D012322:RNA Precursors; D018749:RNA, Plant; D053487:DEAD-box RNA Helicases; C000615652:RH7 protein, Arabidopsis