Fipronil promotes adipogenesis via AMPKα-mediated pathway in 3T3-L1 adipocytes.
Emerging evidence suggests that organochlorine, organophosphorus and neonicotinoid insecticide exposure may be linked to the development of obesity and type 2 diabetes. However, there is no knowledge of the potential influence of fipronil, which belongs to the phenylpyrazole chemical family, on obesity. Thus, the goal of this study was to determine the role of fipronil in adipogenesis using 3T3-L1 adipocytes. Fipronil treatment, at 10 μM, increased fat accumulation in 3T3-L1 adipocytes as well as promoted key regulators of adipocyte differentiation (CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor gamma-γ), and key regulators of lipogenesis (acetyl-CoA carboxylase and fatty acid synthase). The activation of AMPKα with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) abolished effects of fipronil on increased adipogenesis. These results suggest that fipronil alters adipogenesis and results in increased lipid accumulation through a AMPKα-mediated pathway.
Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States.
Quancai
Q
Sun
Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States.
Weipeng
W
Qi
Amherst Regional High School, Amherst, MA 01003, United States.
Jeremy J
JJ
Yang
Department of Biological Sciences and Environmental Sciences Program, Southern Illinois University, Edwardsville, IL 62026, United States.
Kyong Sup
KS
Yoon
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, United States.
John M
JM
Clark
Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States. Electronic address: ypark@foodsci.umass.edu.
Yeonhwa
Y
Park
D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects / Q000502:physiology*; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D015151:Immunoblotting; D007306:Insecticides / Q000494:pharmacology; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012265:Ribonucleotides / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014280:Triglycerides / Q000378:metabolism*
D022763:CCAAT-Enhancer-Binding Protein-alpha; D007306:Insecticides; D047495:PPAR gamma; D011720:Pyrazoles; D012333:RNA, Messenger; D012265:Ribonucleotides; D014280:Triglycerides; D000620:Aminoimidazole Carboxamide; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase; C031143:AICA ribonucleotide; C082360:fipronil
AMPKα; Adipogenesis; Fipronil; Lipid metabolism; Phenylpyrazole