Effects of vitamin D3 and vitamin E on prednisolone-induced alterations of phagocyte function.
OBJECTIVE
To evaluate the effectiveness of vitamin D3 and its combined action with vitamin E in the correction of the impairments of phagocyte function caused by chronic glucocorticoid administration.


METHODS
Phagocytic activity was assessed by the ability of peripheral blood neutrophils and monocytes to capture FITC-labeled Escherichia coli using flow cytometry. Metabolic activity of neutrophils was measured cytochemically as nitro blue tetrazolium (NBT) reduction test. Intracellular reactive oxygen species (ROS) were detected by 2',7'-dichlorofluorescein fluorescence.


RESULTS
Prednisolone administration (5 mg/kg b.w., 30 days) was accompanied by vitamin D3 deficiency and decompensation of phagocyte function associated with antimicrobial activity (decrease in NBT reduction rate, ROS formation and phagocytic activity). Vitamin D3 co-administration with prednisolone and, to a greater extent, its combination with α-tocopherol (100 IU and 0.5 mg/rat, 30 days respectively) partially restored phagocyte function.


CONCLUSIONS
These data suggest that vitamin D3 and α-tocopherol can prevent immunosuppressive effects of prednisolone through elevating the efficacy of oxygen-dependent mechanisms of phagocytosis and increasing the functional activity of phagocytic cells.
O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine. ishymansk@inbox.ru.
I O
IO
Shymanskyy
O O
OO
Lisakovska
A O
AO
Mazanova
V M
VM
Riasniy
M M
MM
Veliky
D000818:Animals; D002762:Cholecalciferol / Q000097:blood / Q000494:pharmacology*; D004926:Escherichia coli / Q000737:chemistry / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D011239:Prednisolone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D017382:Reactive Oxygen Species; D014810:Vitamin E; D002762:Cholecalciferol; D011239:Prednisolone; D016650:Fluorescein-5-isothiocyanate