Enhanced early-life nutrition of Holstein bulls increases sperm production potential without decreasing postpubertal semen quality.
Enhanced early-life nutrition (∼130% of required energy and protein) increased testes size and weight (∼20-25%) and reduced age at puberty (∼1 month) in beef and dairy bulls, compared with those fed 70% of dietary requirements. The objective was to determine effects of early-life (2-31 weeks) nutritional modulation on feed costs, predicted number of harvestable sperm and doses of semen, and semen quality. Calves (∼1 week old) were randomly allocated into three groups that were fed 4, 6, or 8 L/day of milk (low [n = 8], medium [n = 9], and high groups [n = 9], respectively) from ages 2 to 8 weeks. Thereafter, they were weaned, transitioned onto barley silage-based diets, to receive ∼70, 100, or 130% of recommended amounts of energy and protein (feed costs were ∼CDN$280 more per bull to feed high versus low diets from 2 to 31 weeks). After 31 weeks, all bulls were fed a medium diet. Semen was collected, by electroejaculation, from 51 to 73 weeks, extended, chilled, and cryopreserved. Bulls fed high nutrition were numerically younger (P = 0.45) at sexual maturity (sperm with ≥30% progressive motility, ≥70% morphologically normal, and ≤20% abnormal heads), first acceptable post-chill sperm motility (>50%; P = 0.66) and first acceptable post-thaw motility (>25% progressive; P = 0.25) than bulls in the low-nutrition group. Semen from three bulls per group was used for in vitro fertilization (total of 1249 bovine oocytes); there were no significant differences among groups in fertilization percentage (mean ± SEM of 68.0 ± 8.7, 77.1 ± 3.5, and 68.7 ± 4.5% for low, medium, and high, respectively) or blastocyst yield (31.5 ± 5.6, 41.4 ± 4.9, and 33.7 ± 4.6%). On the basis of analysis of 2D gels of sperm proteins, 380 spots were identified on the fused master gel, but no spots were differentially expressed across groups. Overall, there were no significant differences in semen quality or sperm function among bulls fed three levels of nutrition from ages 2 to 31 weeks. However, bulls fed high-nutrition early in life had potential to produce more sperm that could be harvested and sold, which would increase profitability, thereby supporting enhanced early-life nutrition as a management tool to improve reproductive potential of dairy bulls.
Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
Alysha
A
Dance
Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada.
Jacob
J
Thundathil
L'Alliance Boviteq Inc., Saint-Hyacinthe, Quebec, Canada.
Patrick
P
Blondin
Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada. Electronic address: jpkastel@ucalgary.ca.
John
J
Kastelic
D000375:Aging; D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle / Q000502:physiology*; D002470:Cell Survival; D015925:Cryopreservation / Q000662:veterinary; D004032:Diet / Q000662:veterinary*; D005615:Freezing; D008297:Male; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation / Q000662:veterinary; D012741:Sexual Maturation / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
Diet; Fertility; IVF; Male reproductive performance; Sperm quality