Effect of kisspeptin challenge on testosterone and inhibin secretion from in vitro testicular tissue of adult male rhesus monkey (Macaca mulatta).
Kisspeptin expression has been found in gonads but a direct role of kisspeptin in reproduction is not known. The objective of this study was to find a dose and time related effect of kisspeptin on testicular hormones secretion of adult male rhesus monkey (n = 5). Kisspeptin (1, 10, 100, 1000 pm) was incubated to a culture of testes (100 mg fragments) of male rhesus monkey and medium for hormone (testosterone and inhibin) measurement was collected after 30, 60 and 120 min. 10 IU hCG (180 min) and 50 ng FSH (60 and 120 min) were incubated to the culture for checking testicular cells ability to secrete hormones in vitro. Kisspeptin did not significantly (P < 0.05) increase the testosterone and inhibin levels at any dose. However, one way anova at pooled doses showed an increase in testosterone levels and paired t-test at pooled doses showed inhibin decrease after 120 min of incubation suggesting an independent effect of time. hCG and FSH significantly (P < 0.05) increased hormone concentration compared to the basal groups. We concluded that kisspeptin has no role in testicular regulation related to testosterone and inhibin release but kisspeptin may have other roles in testicular regulation.
Faculty of Biological Sciences, Department of Animal Sciences, Qauid-e-Azam University, Islamabad, Pakistan.
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AR
Tariq
Faculty of Biological Sciences, Department of Animal Sciences, Qauid-e-Azam University, Islamabad, Pakistan.
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Shabab
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007265:Inhibins / Q000378:metabolism*; D059648:Kisspeptins / Q000494:pharmacology*; D008253:Macaca mulatta; D008297:Male; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D059648:Kisspeptins; D013739:Testosterone; D007265:Inhibins; D005640:Follicle Stimulating Hormone
Inhibin; kisspeptin; primate; sertoli cells; testis