Mechanistic Insights into Cofactor-Dependent Coupling of RNA Folding and mRNA Transcription/Translation by a Cobalamin Riboswitch.
Riboswitches are mRNA elements regulating gene expression in response to direct binding of a metabolite. While these RNAs are increasingly well understood with respect to interactions between receptor domains and their cognate effector molecules, little is known about the specific mechanistic relationship between metabolite binding and gene regulation by the downstream regulatory domain. Using a combination of cell-based, biochemical, and biophysical techniques, we reveal the specific RNA architectural features enabling a cobalamin-dependent hairpin loop docking interaction between receptor and regulatory domains. Furthermore, these data demonstrate that docking kinetics dictate a regulatory response involving the coupling of translation initiation to general mechanisms that control mRNA abundance. These results yield a comprehensive picture of how RNA structure in the riboswitch regulatory domain enables kinetically constrained ligand-dependent regulation of gene expression.
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Campus Box 596, Boulder, CO 80309-0596, USA.
Jacob T
JT
Polaski
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Campus Box 596, Boulder, CO 80309-0596, USA.
Erik D
ED
Holmstrom
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Campus Box 596, Boulder, CO 80309-0596, USA; JILA, University of Colorado and National Institute of Standards and Technology, Campus Box 440, Boulder, CO 80309-0440, USA.
David J
DJ
Nesbitt
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Campus Box 596, Boulder, CO 80309-0596, USA. Electronic address: robert.batey@colorado.edu.
Robert T
RT
Batey
D001483:Base Sequence; D003067:Coenzymes / Q000378:metabolism*; D005786:Gene Expression Regulation; D007700:Kinetics; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D059370:RNA Folding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058928:Riboswitch / Q000235:genetics*; D014158:Transcription, Genetic; D014805:Vitamin B 12 / Q000378:metabolism*
D003067:Coenzymes; D012333:RNA, Messenger; D058928:Riboswitch; D014805:Vitamin B 12