Comparative analysis of rabbit hemorrhagic disease virus strains originating from outbreaks in the Russian Federation.
Since the first introduction of rabbit hemorrhagic disease (RHD) in 1986, disease outbreaks have been continuously reported in different regions of Russia. Despite extensive vaccination, sporadic RHD cases are still reported. Here, we examine eleven RHDV strains originating from disease outbreaks occurring between 2003 and 2012 and one widely used vaccine strain. Notable phenotypic and genetic heterogeneity among RHDV strains was observed. The RHDV strains Tambov-2010, Perm-2010, Manihino-09 showed different hemagglutinating activity (HA) at 4 °C and room temperature. While all RHDV field strains were identified as hemagglutinating virulent viruses of the RHDVa variant, the vaccine strain was assigned as a "classical" RHDV. These data indicate that since 2003, RHDVa has become the predominant variant circulating in Russia.
Molecular Virology Laboratory, National Research Institute of Veterinary Virology and Microbiology (VNIIVViM), Russian Academy of Agricultural Science, Pokrov, 601125, Russia.
Galina
G
Burmakina
Molecular Virology Laboratory, National Research Institute of Veterinary Virology and Microbiology (VNIIVViM), Russian Academy of Agricultural Science, Pokrov, 601125, Russia.
Nina
N
Malogolovkina
Molecular Virology Laboratory, National Research Institute of Veterinary Virology and Microbiology (VNIIVViM), Russian Academy of Agricultural Science, Pokrov, 601125, Russia.
Andrey
A
Lunitsin
Molecular Virology Laboratory, National Research Institute of Veterinary Virology and Microbiology (VNIIVViM), Russian Academy of Agricultural Science, Pokrov, 601125, Russia.
Ilya
I
Titov
Molecular Virology Laboratory, National Research Institute of Veterinary Virology and Microbiology (VNIIVViM), Russian Academy of Agricultural Science, Pokrov, 601125, Russia.
Sodnom
S
Tsybanov
Molecular Virology Laboratory, National Research Institute of Veterinary Virology and Microbiology (VNIIVViM), Russian Academy of Agricultural Science, Pokrov, 601125, Russia. Malogolovkin@inbox.ru.
Alexander
A
Malogolovkin
D000818:Animals; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks; D017929:Hemorrhagic Disease Virus, Rabbit / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011817:Rabbits / Q000821:virology*; D012426:Russia / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins
Hemagglutinating viruses; Phylogenetic analysis; RHDVa; Rabbit hemorrhagic disease virus; VP60