Inhibition of dengue virus replication by diisopropyl chrysin-7-yl phosphate.
Dengue fever is a tropical disease and caused by dengue virus (DENV), which is transmitted by mosquitoes and infects about 400 million people annually. With the development of international trade and travel, China is facing a growing threat. Over 40 thousands of people were infected during the 2014 DENV outbreak in Guangdong. Neither licensed vaccine nor therapeutic drug has been available. In this report, we isolated two clinical DENV strains. The full-length genome was sequenced and characterized. We also applied a flavonoid, CPI, into an anti-DENV assay. Replication of viral RNA and expression of viral protein was all strongly inhibited. These results indicated that CPI may serve as potential protective agents in the treatment of patients with chronic DENV infection.
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China.
Jiang
J
Du
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China.
Zhe
Z
Chen
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China.
Ting
T
Zhang
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. jianminwang@ipbcams.ac.cn.
Jianmin
J
Wang
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. zdsys@vip.sina.com.
Qi
Q
Jin
D000818:Animals; D002522:Chlorocebus aethiops; D002911:Chrysenes / Q000494:pharmacology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000187:drug effects*; D006801:Humans; D010755:Organophosphates / Q000494:pharmacology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
D002911:Chrysenes; D018076:DNA, Complementary; D010755:Organophosphates; D012367:RNA, Viral; C000594151:diisopropyl chrysin-7-yl phosphate
antiviral activity; dengue virus; flavonoid; phylogenetic analysis; virus isolation