Investigation the Possibility of Using Peptides with a Helical Repeating Pattern of Hydro-Phobic and Hydrophilic Residues to Inhibit IL-10.
Blockade of IL-10 signalling clears chronic viral and bacterial infections. Immunization together with blockade of IL-10 signalling or relatively low level of IL-10 further enhances viral and bacterial clearance. IL-10 functions through binding to interleukin 10 receptor (IL-10R). Here we showed that peptides P1 and P2 with the hydrophobic and hydrophilic pattern of the IL10R-binding helix in IL-10 could bind with either IL-10R1 or IL-10, and inhibit inflammatory signals with long duration and negligible cytotoxicity in vitro. Furthermore, P2 can enhance antigen specific CD8+ T cell responses in mice induced by the vaccine based on a long peptide of protein E7 in a human papillomavirus type 16.
Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Australia.|School of Medical Science, Griffith Health Institute, Griffith University, Gold Coast, Australia.
Guoying
G
Ni
Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong, China.
Shu
S
Chen
The Institute for Glycomics and School of Information and Communication Technology, Griffith University, Gold Coast, Australia.
Yuedong
Y
Yang
Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Australia.
Scott F
SF
Cummins
The Institute for Glycomics and School of Information and Communication Technology, Griffith University, Gold Coast, Australia.
Jian
J
Zhan
Diamantina Institute, University of Queensland, Woolloongabba, Australia.
Zhixiu
Z
Li
Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong, China.
Bin
B
Zhu
Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of Sunshine Coast, Maroochydore DC, Australia.
Kate
K
Mounsey
Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of Sunshine Coast, Maroochydore DC, Australia.
Shelley
S
Walton
School of Medical Science, Griffith Health Institute, Griffith University, Gold Coast, Australia.
Ming Q
MQ
Wei
Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong, China.
Yuejian
Y
Wang
The Institute for Glycomics and School of Information and Communication Technology, Griffith University, Gold Coast, Australia.
Yaoqi
Y
Zhou
Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Australia.
Tianfang
T
Wang
Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Australia.|Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong, China.
Xiaosong
X
Liu
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology; D006801:Humans; D007114:Immunization; D016753:Interleukin-10 / Q000037:antagonists & inhibitors* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000276:immunology*; D030361:Papillomavirus Infections / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control* / Q000821:virology; D053918:Papillomavirus Vaccines / Q000276:immunology*; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020349:Surface Plasmon Resonance; D022223:Vaccines, Subunit / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology*
C508609:IL10 protein, human; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D022223:Vaccines, Subunit; C059731:oncogene protein E7, Human papillomavirus type 16; D016753:Interleukin-10