[Effect of amn gene deletion on Corynebacterium glutamicum S9114 metabolism].
OBJECTIVE
To study the effect of adenosine monophosphate nucleosidase gene deletion on Corynebacterium glutamicum S9114 metabolism.


METHODS
We constructed the amn deficient strain A amn and studied its glutamate fermentation and acid tolerance.


RESULTS
Compared with the wild type strain, biomass of A amn strain increased by 16.2% whereas glutamate production reduced by 58.8%. After 10, 25 and 40 h, intracellular ATP level of A amn strain increased 3.0, 3.7 and 2.2 times. The isocitrate lyase activity increased by 17.1% , 4.9% and 44.5%, the isocitrate dehydrogenase activity decreased by 76.9%, 74.6% and 5.0%, and the glutamate dehydrogenase activity decreased by 42.4%, 50.8% and 42.4%. At pH4.0, the survival of Aamn strain dropped 64.9%. However, the intracellular ROS and protein carbonylation level showed an increase of 31.5% and 22.5% respectively.


CONCLUSIONS
Knocking out of amn gene enhanced intracellular ATP level and biomass formation, whereas had negative effect on glutamate fermentation and acid tolerance.
Jie
J
Mei
Liming
L
Liu
Jianrong
J
Wu
D000659:AMP Deaminase / Q000235:genetics* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005285:Fermentation; D017353:Gene Deletion; D018698:Glutamic Acid / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D000659:AMP Deaminase