Halloysite-based dopamine-imprinted polymer for selective protein capture.
We describe a facile, general, and highly efficient approach to obtain polydopamine-coated molecularly imprinted polymer based on halloysite nanotubes for bovine serum albumin. The method combined surface molecular imprinting and one-step immobilized template technique. Hierarchically structured polymer was prepared in physiological conditions adopting dopamine as functional monomer. A thin layer of polydopamine can be coated on the surface of amino-modified halloysite nanotubes by self-polymerization, and the thickness of the imprinted shells can be controlled by the mass ratio of matrix and dopamine. The polymer was characterized by Fourier transform infrared spectrometry, transmission electron microscopy, and thermogravimetric analysis. The prepared material showed high binding capacity (45.4 mg/g) and specific recognition behavior toward the template protein. In addition, stability and regeneration analyses indicated that the imprinted polymer exhibited excellent reusability (relative standard deviation < 9% for batch-to-batch evaluation). Therefore, the developed polymer is effective for protein recognition and separation.
School of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Xiaohong
X
Zhu
School of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Hui
H
Li
School of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Hui
H
Liu
School of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Wei
W
Peng
School of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Shian
S
Zhong
School of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Yan
Y
Wang
D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D000077215:Clay; D004298:Dopamine / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification*
D000538:Aluminum Silicates; D011108:Polymers; D012710:Serum Albumin, Bovine; D000077215:Clay; D004298:Dopamine
Dopamine; Halloysite nanotubes; Molecularly imprinted polymers; Protein separation; Surface imprinting