Does broodstock nutritional history affect the response of progeny to different first-feeding diets? A whole-body transcriptomic study of rainbow trout alevins.
The whole-body transcriptome of trout alevins was characterised to investigate the effects of long-term feeding of rainbow trout broodstock females a diet free of fishmeal and fish oil on the metabolic capacities of progeny. Effects were studied before first feeding and after 3 weeks of feeding diets containing different proportions of marine and plant ingredients. Feeding alevins plant-based diets resulted in lower fish body weight, irrespective of maternal nutritional history. No differences in whole-body lipids were found between treatments, and the tissue fatty acid profile strongly reflected that of the respective broodstock or first-feeding diets. We showed that the maternal diet history did not significantly affect expressions of any genes before the first feeding. Interestingly, we found an effect of maternal nutritional history on gene expression in alevins after 3 weeks of feeding. The major differences in the transcriptome of alevins from plant-based diet-fed females compared with those from commercial-fed females were as follows: (i) down-regulation of genes involved in muscle growth/contraction and (ii) up-regulation of genes involved in carbohydrate and energy metabolism related to the delay in growth/development observed with plant-based diets. Our findings also showed an effect of the first-feeding diets, irrespective of maternal nutritional history. Specifically, the introduction of plant ingredients resulted in the up-regulation of genes involved in amino acid/protein and cholesterol metabolism and in differences in the expressions of genes related to carbohydrate metabolism. Information gained through this study opens up avenues for further reduction of marine ingredients in trout diets, including the whole rearing cycle.
1INRA-UR 1067'Nutrition Métabolisme Aquaculture',Aquapôle,64310 Saint-Pée-sur-Nivelle,France.
Viviana
V
Lazzarotto
1INRA-UR 1067'Nutrition Métabolisme Aquaculture',Aquapôle,64310 Saint-Pée-sur-Nivelle,France.
Geneviève
G
Corraze
1INRA-UR 1067'Nutrition Métabolisme Aquaculture',Aquapôle,64310 Saint-Pée-sur-Nivelle,France.
Laurence
L
Larroquet
2IFREMER - LEMAR UMR 6539,CNRS-UBO-IRD-Ifremer,ZI de la Pointe du Diable,CS 10070,29280 Plouzané,France.
David
D
Mazurais
1INRA-UR 1067'Nutrition Métabolisme Aquaculture',Aquapôle,64310 Saint-Pée-sur-Nivelle,France.
Françoise
F
Médale
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn; D017756:Aquaculture; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D014676:Diet, Vegetarian; D004040:Dietary Carbohydrates / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D004734:Energy Metabolism / Q000235:genetics; D044127:Epigenesis, Genetic; D005260:Female; D039382:Maternal Nutritional Physiological Phenomena; D009132:Muscles / Q000502:physiology; D009752:Nutritional Status; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010944:Plants; D011247:Pregnancy; D059467:Transcriptome
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004044:Dietary Proteins
C diet commercial-like FM-FO and plant-based diet; COM commercial diet; Cy3 Cyanine 3-CTP; Early stages; FA fatty acid; FC fold change; FM fishmeal; FO fish oil; Fatty acids; Fish; GCK glucokinase; GO gene ontology; Gene expression; LC-PUFA long chain PUFA; M diet marine FM-FO-based diet; Microarrays; NL neutral lipid; Nutrition; PL polar lipid; Plant products; V diet experimentaly 100 % plant-based diet; VEG plant-based diet; cRNA complementary RNA