Chemiluminescence Detection of Nitric Oxide from Roots, Leaves, and Root Mitochondria.
NO is a free radical with short half-life and high reactivity; due to its physiochemical properties it is very difficult to detect the concentrations precisely. Chemiluminescence is one of the robust methods to quantify NO. Detection of NO by this method is based on reaction of nitric oxide with ozone which leads to emission of light and amount of light is proportional to NO. By this method NO can be measured in the range of pico moles to nano moles range. Using direct chemiluminescence method, NO emitted into the gas stream can be detected whereas using indirect chemiluminescence oxidized forms of NO can also be detected. We detected NO using purified nitrate reductase, mitochondria, cell suspensions, and roots; detail measurement method is described here.
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India.
Aakanksha
A
Wany
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India.
Alok Kumar
AK
Gupta
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India.
Aprajita
A
Kumari
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India.
Shika
S
Gupta
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India.
Sonal
S
Mishra
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India.
Ritika
R
Jaintu
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India.
Pradeep K
PK
Pathak
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India. jgk@nipgr.ac.in.
Kapuganti Jagadis
KJ
Gupta
D049449:Luminescence; D008928:Mitochondria / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism
D009569:Nitric Oxide
Cell suspensions; Chemiluminescence; Hydroponics; Mitochondria; Nitric oxide