Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds.
Establishment of an efficient explants surface disinfection protocol is essential for in vitro cell and tissue culture as well as germplasm conservation, such as the case of Grapevine (Vitis spp.) culture. In this research, different procedures for disinfection and regeneration of field-grown grapevine cv. 'Flame seedless' axillary buds were evaluated. The buds were disinfected using either NaOCl or allyl, benzyl, phenyl and 2-phenylethyl isothiocyanates. Two different media for shooting and four media for rooting were tested. Shoot and root development per buds were registered. The best disinfection procedure with 90 % of tissue survival involved shaking for 60 min in a solution containing 20 % Clorox with 50 drops/L Triton(®) X-100. These tissues showed the potential to regenerate a complete plant. Plant regeneration was conducted using full strength Murashigue and Skoog (MS) medium supplemented with 8 µM benzyl aminopurine for shoot induction and multiplication, whereas rooting was obtained on half strength MS supplemented with 2 mg L(-1) of indole-3-butyric acid and 200 mg L(-1) of activated charcoal. In this work, it was designed the protocols for obtaining sterile field-grown grapevine buds and in vitro plant development. This methodology showed potential to produce vigorous and healthy plants in 5 weeks for clonal grapevine propagation. Regenerated plants were successfully established in soil.
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera a La Victoria Km 0.6, 83304 Hermosillo, Sonora México.
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Lazo-Javalera
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera La Victoria Km 0.6, 83304 Hermosillo, Sonora México.
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Troncoso-Rojas
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera La Victoria Km 0.6, 83304 Hermosillo, Sonora México.
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Tiznado-Hernández
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera La Victoria Km 0.6, 83304 Hermosillo, Sonora México.
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Martínez-Tellez
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera a La Victoria Km 0.6, 83304 Hermosillo, Sonora México.
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Vargas-Arispuro
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera La Victoria Km 0.6, 83304 Hermosillo, Sonora México.
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Islas-Osuna
Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera a La Victoria Km 0.6, 83304 Hermosillo, Sonora México.
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Rivera-Domínguez
Clonal propagation; Flame seedless; Grape; Isothiocyanate; Micropropagation; Organogenesis