Serum of Blood Separation by Means of Dynamic Electrochromatography in a Centrifugal and Electromagnetic Gradient.
The paper presents the dynamic association of several gradients of separation with electrophoresis and the laboratory implementation of a prototype device for dynamic electrochromatography in a centrifugal and electromagnetic gradient (DECGOE), based on patent PL171643. The object of the study was to compare the separation capacity and repeatability of the location of edge parameters (xx(1), yy(1), d) of the contours of a proteinogram fraction for conventional electrophoresis (EF), radial SR-DECGOE separation and three variants of radial-transverse SR-SPM, SR-SPE, SRSPRRM distribution as well as the reproducibility of the following prototype parameters: PV(1), PV(2), PV(3)[V] potentials, PV(3)[F] frequency, ω(1) and ω(2) rotational speed, MC mixer chamber buffer [pH] and volume[ml], and B [T] magnetic induction. Human blood serum was used in the study, 50 samples for each separation type. The highest rate of fraction formation was registered for the SR-SPRRM (8,07/s) variant. The percentage values of standard deviations for the edge parameters of proteinograms were the lowest for conventional electrophoresis (EF), slightly higher for radial distribution SR, while among the radial-transverse separations, the highest accuracy was obtained for the SR-SPRRM variant.
LABIOT Laboratory of Biotechnology Cieszyn, Poland.
Andrzej
A
Jan Dyszkiewicz
D001769:Blood; D002498:Centrifugation / Q000379:methods*; D055590:Electromagnetic Phenomena; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D010330:Patents as Topic; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry*