Effects of chlordecone on 20-hydroxyecdysone concentration and chitobiase activity in a decapod crustacean, Macrobrachium rosenbergii.
Chlordecone (CLD) is an organochlorine insecticide abundant in aquatic environment of the French West Indies. However, few studies have investigated its impact on freshwater invertebrates. Whereas CLD is suspected of inducing endocrine disruption, this work aimed to study the effects of environmentally relevant concentrations of CLD on the 20-hydroxyecdysone (20-HE) hormone concentration and on the chitobiase activity, both having key roles in the molting process of crustaceans. In addition, the bioaccumulation of CLD was measured in the muscle tissue of Macrobrachium rosenbergii to underline potential dose-response relationship. The results have shown that CLD was bioaccumulated in exposed organisms according to a trend to a dose-response relationship. Moreover, it was observed that CLD decreased the 20-HE concentration in exposed prawns when compared to control, whatever the duration of exposure, as well as it inhibited the chitobiase activity after 30days of exposure. The present study indicates that CLD could interfere with molting process of M. rosenbergii by disturbing the 20-HE concentration and the activity of chitobiase, suggesting consequences at the long term on the shrimp development. This study also confirmed that CLD could be an endocrine disruptor in decapod crustaceans, as it was already observed in vertebrates.
University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre of Analytical Research and Technology (CART), 15 Allée du Six Aout, B-4000 Liège, Belgium. Electronic address: Anne.Lafontaine@ulg.ac.be.
Anne
A
Lafontaine
University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre of Analytical Research and Technology (CART), 15 Allée du Six Aout, B-4000 Liège, Belgium.
Eric
E
Gismondi
Normandie University, ULH, UMR I-02, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-FR CNRS 3730 SCALE, 25 rue Philippe Lebon, F-76600 Le Havre, France.
Céline
C
Boulangé-Lecomte
Akvaplan-Niva (Norwegian Institute of Water Research) AS, Fram Centre, 9296 Tromsoe, Norway.
Perrine
P
Geraudie
University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre of Analytical Research and Technology (CART), 15 Allée du Six Aout, B-4000 Liège, Belgium.
Nathalie
N
Dodet
DYNECAR-UMR BOREA (MNHN/CNRS 7208/IRD207/UPMC), University of the French West Indies and Guiana, Campus de Fouillole, Pointe-à-Pitre, Guadeloupe F-97110, France; INRA, UMR0985 Ecology and Ecosystem Health Research Unit, Ecotoxicology and Quality of Aquatic Environments Research Group, 65 rue de Saint Brieuc, F-35042 Rennes, France.
Fanny
F
Caupos
DYNECAR-UMR BOREA (MNHN/CNRS 7208/IRD207/UPMC), University of the French West Indies and Guiana, Campus de Fouillole, Pointe-à-Pitre, Guadeloupe F-97110, France.
Soazig
S
Lemoine
INRA, UMR0985 Ecology and Ecosystem Health Research Unit, Ecotoxicology and Quality of Aquatic Environments Research Group, 65 rue de Saint Brieuc, F-35042 Rennes, France.
Laurent
L
Lagadic
University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre of Analytical Research and Technology (CART), 15 Allée du Six Aout, B-4000 Liège, Belgium.
Jean-Pierre
JP
Thomé
Normandie University, ULH, UMR I-02, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-FR CNRS 3730 SCALE, 25 rue Philippe Lebon, F-76600 Le Havre, France.
Joëlle
J
Forget-Leray
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000818:Animals; D007631:Chlordecone / Q000633:toxicity*; D004441:Ecdysterone / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity; D004781:Environmental Exposure; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D033541:Palaemonidae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
D052244:Endocrine Disruptors; D007306:Insecticides; D014874:Water Pollutants, Chemical; D004441:Ecdysterone; D000118:Acetylglucosaminidase; D007631:Chlordecone
20-Hydroxyecdysone; Chitobiase; Chlordecone; Endocrine disruptors; Macrobrachium rosenbergii