A mobile genetic element profoundly increases heat resistance of bacterial spores.
Bacterial endospores are among the most resilient forms of life on earth and are intrinsically resistant to extreme environments and antimicrobial treatments. Their resilience is explained by unique cellular structures formed by a complex developmental process often initiated in response to nutrient deprivation. Although the macromolecular structures of spores from different bacterial species are similar, their resistance to environmental insults differs widely. It is not known which of the factors attributed to spore resistance confer very high-level heat resistance. Here, we provide conclusive evidence that in Bacillus subtilis, this is due to the presence of a mobile genetic element (Tn1546-like) carrying five predicted operons, one of which contains genes that encode homologs of SpoVAC, SpoVAD and SpoVAEb and four other genes encoding proteins with unknown functions. This operon, named spoVA2mob, confers high-level heat resistance to spores. Deletion of spoVA2mobin a B. subtilis strain carrying Tn1546 renders heat-sensitive spores while transfer of spoVA2mobinto B. subtilis 168 yields highly heat-resistant spores. On the basis of the genetic conservation of different spoVA operons among spore-forming species of Bacillaceae, we propose an evolutionary scenario for the emergence of extremely heat-resistant spores in B. subtilis, B. licheniformis and B. amyloliquefaciens. This discovery opens up avenues for improved detection and control of spore-forming bacteria able to produce highly heat-resistant spores.
Top Institute Food and Nutrition (TIFN), Wageningen, The Netherlands.|University of Groningen, Laboratory of Molecular Genetics, Groningen, The Netherlands.|NIZO Food Research B.V., Ede, The Netherlands.
Erwin M
EM
Berendsen
Top Institute Food and Nutrition (TIFN), Wageningen, The Netherlands.|NIZO Food Research B.V., Ede, The Netherlands.
Jos
J
Boekhorst
Top Institute Food and Nutrition (TIFN), Wageningen, The Netherlands.|University of Groningen, Laboratory of Molecular Genetics, Groningen, The Netherlands.
Oscar P
OP
Kuipers
Top Institute Food and Nutrition (TIFN), Wageningen, The Netherlands.|NIZO Food Research B.V., Ede, The Netherlands.
Marjon H J
MH
Wells-Bennik
D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D006358:Hot Temperature; D009876:Operon; D013171:Spores, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements