Calcium signaling and copper toxicity in Saccharomyces cerevisiae cells.
To respond to metal surpluses, cells have developed intricate ways of defense against the excessive metallic ions. To understand the ways in which cells sense the presence of toxic concentration in the environment, the role of Ca2+in mediating the cell response to high Cu2+was investigated in Saccharomyces cerevisiae cells. It was found that the cell exposure to high Cu2+was accompanied by elevations in cytosolic Ca2+with patterns that were influenced not only by Cu2+concentration but also by the oxidative state of the cell. When Ca2+channel deletion mutants were used, it was revealed that the main contributor to the cytosolic Ca2+pool under Cu2+stress was the vacuolar Ca2+channel, Yvc1, also activated by the Cch1-mediated Ca2+influx. Using yeast mutants defective in the Cu2+transport across the plasma membrane, it was found that the Cu2+-dependent Ca2+elevation could correlate not only with the accumulated metal, but also with the overall oxidative status. Moreover, it was revealed that Cu2+and H2O2acted in synergy to induce Ca2+-mediated responses to external stress.
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.
Lavinia L
LL
Ruta
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.
Claudia V
CV
Popa
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania.
Ioana
I
Nicolau
Faculty of Chemistry, University of Bucharest, Sos. Panduri 90-92, Bucharest, Romania. ileana.farcasanu@chimie.unibuc.ro.
Ileana C
IC
Farcasanu
D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D003300:Copper / Q000378:metabolism* / Q000633:toxicity; D003600:Cytosol / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; D006861:Hydrogen Peroxide; D002118:Calcium
Aequorin; Calcium signaling; Copper; H2O2; Saccharomyces cerevisiae