Identification of potential biomarkers for post-traumatic complications released after trauma-hemorrhage from murine Kupffer cells and its investigation in lung and liver.
BACKGROUND
Early diagnosis of complications after severe trauma by specific biomarkers remains difficult.


OBJECTIVE
Identify potential new biomarkers for early diagnosis of post-traumatic complications.


METHODS
Mice underwent pressure-controlled hemorrhage or sham procedure. Four hours later, genome-wide expression of isolated Kupffer cells was compared with controls using Affymetrix-Genechip-Expression-Analysis and real-time-PCR.


RESULTS
Expression analysis and real-time-PCR revealed a significant increase of gene expression of Cxcl10, Il4ra, Csf2rb2, Lcn2, and Gbp5.


CONCLUSIONS
Cxcl10, Il4ra, Csf2rb2, Lcn2, and Gbp5 might represent new biomarkers for early diagnosis of post-traumatic complications, if they are linked to the development of post-traumatic complications.
a Trauma Department , Hannover Medical School , Hannover , Germany ;
Cornelia
C
Schultze
b Department of Orthopaedics and Trauma Surgery , University Hospital Aachen , Aachen , Germany.
Frank
F
Hildebrand
a Trauma Department , Hannover Medical School , Hannover , Germany ;
Sandra
S
Noack
a Trauma Department , Hannover Medical School , Hannover , Germany ;
Christian
C
Krettek
a Trauma Department , Hannover Medical School , Hannover , Germany ;
Christian
C
Zeckey
a Trauma Department , Hannover Medical School , Hannover , Germany ;
Claudia
C
Neunaber
D000818:Animals; D015415:Biomarkers; D054357:Chemokine CXCL10 / Q000032:analysis; D019204:GTP-Binding Proteins / Q000032:analysis; D055106:Genome-Wide Association Study; D006470:Hemorrhage / Q000378:metabolism*; D007728:Kupffer Cells / Q000378:metabolism*; D000071068:Lipocalin-2 / Q000032:analysis; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis; D016185:Receptors, Interleukin-3 / Q000032:analysis; D015854:Up-Regulation; D014947:Wounds and Injuries / Q000150:complications*; D053662:Interleukin-4 Receptor alpha Subunit
D015415:Biomarkers; D054357:Chemokine CXCL10; D019204:GTP-Binding Proteins; D000071068:Lipocalin-2; D011956:Receptors, Cell Surface; D016185:Receptors, Interleukin-3; C485433:Cxcl10 protein, mouse; C504002:Il4ra protein, mouse; D053662:Interleukin-4 Receptor alpha Subunit; C071332:Lcn2 protein, mouse; C072185:Csf2rb2 protein, mouse; C472531:Gbp5 protein, mouse
Csf2rb2; Cxcl10; Gbp5; Il4ra; Lcn2; multiple trauma