Metastasis-associated lung adenocarcinoma transcript 1 promotes the proliferation of chondrosarcoma cell via activating Notch-1 signaling pathway.
BACKGROUND
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) is identified to be overexpressed in several cancers. However, the role of MALAT-1 in chondrosarcoma is poorly understood.


METHODS
The expression of MALAT-1 and Notch-1 signaling pathway was detected in chondrosarcoma tissues and chondrosarcoma cells by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was performed to examine the cell viability of chondrosarcoma cells transfected with si-MALAT-1 or pcDNA-MALAT-1. Then the expression of Notch-1 signaling pathway was detected when MALAT-1 was upregulated or downregulated in chondrosarcoma cells. A subcutaneous chondrosarcoma cells xenograft model was used to confirm the effect of MALAT-1 on tumor growth in vivo.


RESULTS
We found the increased expression of MALAT-1 and Notch-1 signaling pathway in chondrosarcoma tissue and cells. MALAT-1 promoted the proliferation of chondrosarcoma cells. In addition, MALAT-1 activated the Notch-1 signaling pathway at posttranscriptional level in chondrosarcoma cells. Meanwhile, overexpression of Notch-1 reversed the effect of si-MALAT-1 on the proliferation of chondrosarcoma cells. Finally, we found that MALAT-1 promoted the tumor growth in a subcutaneous chondrosarcoma cells xenograft model, which confirmed the promoted effect of MALAT-1 on the tumor growth in vivo.


CONCLUSIONS
Taken together, our study demonstrated that MALAT-1 promoted the proliferation of chondrosarcoma cell via activating Notch-1 signaling pathway.
Department of Orthopaedics, Hongquan Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Fengqin
F
Xu
Department of Orthopaedics, Subei People's Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Zhi-Qiang
ZQ
Zhang
Department of Orthopaedics, Subei People's Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Yong-Chao
YC
Fang
Department of Orthopaedics, Subei People's Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Xiao-Lei
XL
Li
Department of Orthopaedics, Subei People's Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Yu
Y
Sun
Department of Orthopaedics, Subei People's Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Chuan-Zhi
CZ
Xiong
Department of Orthopaedics, Subei People's Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Lian-Qi
LQ
Yan
Department of Orthopaedics, Subei People's Hospital, Yangzhou, Jiangsu Province, People's Republic of China.
Qiang
Q
Wang
MALAT-1; Notch-1; cell proliferation; chondrosarcoma; molecular pathophysiology