Antibiofilm efficacy of photoactivated curcumin, triple and double antibiotic paste, 2% chlorhexidine and calcium hydroxide against Enterococcus fecalis in vitro.
Root canal disinfection is one of the most important factors governing success of root canal treatment, especially when regenerative strategies are used. This study evaluated the efficacy of 5 intracanal medicaments against mature biofilms of Enterococcus fecalis in vitro: Light activated curcumin, triple antibiotic paste (TAP), double antibiotic paste (DAP), chlorhexidine, calcium hydroxide. Untreated teeth with biofilms served as controls. Confocal microscopy was used to analyse the biofilm mass and percentage of live/dead bacteria within the root canal as well as dentinal tubules. Dentinal shavings obtained from the root canal walls (at 200 and 400 microns depth) were used to quantify the colony forming units/mL. The results showed that light activated curcumin and triple antibiotic paste brought about complete disruption of the biofilm structure (P < 0.05) while chlorhexidine and calcium hydroxide were not significantly different from the control (P > 0.05). Light activated curcumin brought about the highest percentage of dead cells at both depths, but this was not significantly different from triple antibiotic paste (P > 0.05). Curcumin, TAP and DAP brought about a significant reduction of CFU/mL at both depths compared to the control and other groups (P < 0.05). Light activated curcumin brought about a 7 log reduction of bacteria at both depths.
Biofilm Research Cluster, Saveetha Dental College and Hospitals, Saveetha University, Chennai, India.
Sharmila
S
Devaraj
Department of Oral and Maxillofacial Pathology. Saveetha Dental College and Hospitals, Saveetha University, Chennai, India.
Nithya
N
Jagannathan
Biofilm Research Cluster, Saveetha Dental College and Hospitals, Saveetha University, Chennai, India.|Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha University, Chennai, India.
Prasanna
P
Neelakantan
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D009824:Ointments
D000900:Anti-Bacterial Agents; D009824:Ointments; D003474:Curcumin; D002126:Calcium Hydroxide; D002710:Chlorhexidine