Acute Administration of Diazepam Provokes Redox Homeostasis Imbalance in the Rat Brain: Prevention by Simvastatin.
We investigated the effects of acute diazepam (DZP) administration on thiobarbituric acid-reactive substance (TBARS) levels, protein carbonyl content, and on the activities of the antioxidant enzymes catalase, glutathione peroxidase, and superoxide dismutase in the brain of rats. Additionally, we investigated the antioxidant role of chronic pretreatment with simvastatin on the effects provoked by DZP. Simvastatin was administered (1 or 10 mg/kg by oral gavage) for 30 days. On the 30th day of treatment, groups were randomized and DZP was administered (0.5 or 1.0 mg/kg by intraperitoneal injection). Control groups received saline. Results showed that DZP enhanced TBARS levels and protein carbonyl content and altered enzymatic activity in the brain of rats. Simvastatin prevented most of the alterations caused by DZP on the oxidative stress parameters. Data indicate that DZP administration causes an oxidative imbalance in the brain areas studied; however, in the presence of simvastatin, some of these alterations in oxidative stress were prevented.
Department of Medicine, Regional University of Blumenau, Rua Antônio da Veiga, 140, Blumenau, SC, Brazil.
Guilherme André
GA
Eger
Department of Medicine, Regional University of Blumenau, Rua Antônio da Veiga, 140, Blumenau, SC, Brazil.
Vinícius Vialle
VV
Ferreira
Department of Medicine, Regional University of Blumenau, Rua Antônio da Veiga, 140, Blumenau, SC, Brazil.
Camila Ribeiro
CR
Batista
Department of Medicine, Regional University of Blumenau, Rua Antônio da Veiga, 140, Blumenau, SC, Brazil.
Henrique LuisPetrek
HL
Bonde
Department of Medicine, University of Joinville Region, Campus Universitário, Bairro Bom Retiro, Joinville, SC, Brazil.
Daniela Delwing
DD
de Lima
Department of Natural Sciences, Regional University of Blumenau, Rua Antônio da Veiga, Blumenau, SC, Brazil.
André Felipe
AF
Rodrigues
Department of Natural Sciences, Regional University of Blumenau, Rua Antônio da Veiga, Blumenau, SC, Brazil.
José Geraldo Pereira
JG
da Cruz
Department of Natural Sciences, Regional University of Blumenau, Rua Antônio da Veiga, Blumenau, SC, Brazil. deboradelwing@furb.br.
Débora Delwing Dal
DD
Magro
D000284:Administration, Oral; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003975:Diazepam / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D004334:Drug Administration Schedule; D005979:Glutathione Peroxidase / Q000378:metabolism; D006993:Hypnotics and Sedatives / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000924:Anticholesteremic Agents; D006993:Hypnotics and Sedatives; D017392:Thiobarbituric Acid Reactive Substances; D019821:Simvastatin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003975:Diazepam
Antioxidant Enzymes; Brain; Diazepam; Lipid Peroxidation; Simvastatin