Assessment of Coriolopsis gallica-treated olive mill wastewater phytotoxicity on tomato plants.
The aim of the present study was to evaluate the phytotoxicity of olive mill wastewater (OMW) after being treated by the white-rot fungus Coriolopsis gallica. For this, the effect of irrigation with treated OMW (TOMW) and untreated OMW (UOMW) on tomato plants (Lycopersicon esculentum) for 3 weeks was studied. The control plants were irrigated with distilled water. Agronomic tests were performed in pot experiments in a greenhouse using the randomized complete block (RCB) experimental design. The relative leaf height (RLH), as a morphological parameter, and the content of total phenols in the roots and total chlorophyll [Cha + Chb] and reducing sugars in the leaves, as physiological parameters, were selected as responses of the experimental design. The results obtained showed that [Cha + Chb] in the leaves of tomato growth under TOMW was enhanced by 36.3 and 19.4 % compared to the plant growth under UOMW and to the controls, respectively. Also, reducing sugar concentrations were closed to those of the control plants, ranging from 0.424 to 0.678 g/L for the different dilutions tested. However, the plants irrigated with UOMW showed lower reducing sugar concentrations ranging from 0.042 to 0.297g/L. The optimum RLH (0.537) was observed in the plants irrigated with TOMW diluted at (1:4), this value being higher than that observed in the controls (0.438). Our study proved that the irrigation with TOMW significantly improved tomato growth and photosynthesis activity over those irrigated with UOMW. Optimization of TOMW as a fertilizer was obtained for a dilution of 1:4. From the obtained results, it can be concluded that OMW treated by C. gallica holds potential to be used as a fertilizer for tomato plants. Graphical Abstract ᅟ Please provide a caption for the graphical abstract.The graphical abstract is improved and sent as attachment Please replace it.
Enzyme Engineering and Microbiology Laboratory, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia. daleldaassi@yahoo.com.|Department of Biology, Faculty of Sciences and Arts, Khulais, University Of Jeddah, Jeddah, Saudi Arabia. daleldaassi@yahoo.com.|Ecole Nationale d'Ingénieurs de Sfax, Route de Soukra Km 4.5 BP 11733038, Sfax, Tunisia. daleldaassi@yahoo.com.
Dalel
D
Daâssi
Enzyme Engineering and Microbiology Laboratory, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.
Sahar
S
Sellami
Department of Biology, Faculty of sciences of Sfax, University of Sfax,, Sfax, Tunisia.
Fakher
F
Frikha
CEIT-IK4, Unit of Environmental Engineering, Paseo Manuel de Lardizábal 15, 20018, San Sebastian, Spain.|IKERBASQUE, Basque Foundation for Science, María Diaz de Haro 3, 48013, Bilbao, Spain.
Susana
S
Rodriguez-Couto
Enzyme Engineering and Microbiology Laboratory, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.
Moncef
M
Nasri
Enzyme Engineering and Microbiology Laboratory, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.
Tahar
T
Mechichi
D001673:Biodegradation, Environmental; D055453:Coriolaceae / Q000502:physiology*; D007220:Industrial Waste / Q000032:analysis; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000254:growth & development; D031658:Olea / Q000737:chemistry; D010788:Photosynthesis; D062065:Wastewater / Q000032:analysis / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D018508:Water Purification
D007220:Industrial Waste; D062065:Wastewater; D014874:Water Pollutants, Chemical
Fertilizer; Fungal treatment; Olive mill wastewater; Phytotoxicity