Microbial community structures and metabolic profiles response differently to physiochemical properties between three landfill cover soils.
Landfills are always the most important part of solid waste management and bear diverse metabolic activities involved in element biogeochemical cycling. There is an increasing interest in understanding the microbial community and activities in landfill cover soils. To improve our knowledge of landfill ecosystems, we determined the microbial physiological profiles and communities in three landfill cover soils (Ninghai: NH, Xiangshan: XS, and Fenghua: FH) of different ages using the MicroResp(TM), phospholipid fatty acid (PLFA), and high-throughput sequencing techniques. Both total PLFAs and glucose-induced respiration suggested more active microorganisms occurred in intermediate cover soils. Microorganisms in all landfill cover soils favored L-malic acid, ketoglutarate, and citric acid. Gram-negative bacterial PLFAs predominated in all samples with the representation of 16:1ω7, 18:1ω7, and cy19:0 in XS and NH sites. Proteobacteria dominated soil microbial phyla across different sites, soil layers, and season samples. Canonical correspondence analysis showed soil pH, dissolved organic C (DOC), As, and total nitrogen (TN) contents significantly influenced the microbial community but TN affected the microbial physiological activities in both summer and winter landfill cover soils.
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, No. 1799 Jimei Road, Xiamen, 361021, China.|Ningbo Key Lab of Urban Environment Process and Pollution Control, Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, No. 88 Zhong Ke Road, Ningbo, 315830, China.
Xi-En
XE
Long
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, No. 1799 Jimei Road, Xiamen, 361021, China.|Ningbo Key Lab of Urban Environment Process and Pollution Control, Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, No. 88 Zhong Ke Road, Ningbo, 315830, China.
Juan
J
Wang
Nanjing Institute of Agricultural Sciences in Jiangsu Hilly Area, No. 6 Xianyin South Road, Qixia District, Nanjing, Jiangsu Province, 210046, China.
Ying
Y
Huang
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, No. 1799 Jimei Road, Xiamen, 361021, China. hyyao@iue.ac.cn.|Ningbo Key Lab of Urban Environment Process and Pollution Control, Ningbo Urban Environment Observation and Research Station-NUEORS, Chinese Academy of Sciences, No. 88 Zhong Ke Road, Ningbo, 315830, China. hyyao@iue.ac.cn.
Huaiying
H
Yao
D002681:China; D017753:Ecosystem; D005227:Fatty Acids / Q000032:analysis; D055442:Metabolome; D059013:Microbial Consortia; D009584:Nitrogen / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D020560:Proteobacteria / Q000254:growth & development / Q000378:metabolism; D012621:Seasons; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D064091:Waste Disposal Facilities
D005227:Fatty Acids; D010743:Phospholipids; D012987:Soil; D009584:Nitrogen
High throughput sequencing; Landfill cover soil; MicroRespTM; Microbial community; PLFA; Physiological profile