Comparative Cold Shock Expression and Characterization of Fungal Dye-Decolorizing Peroxidases.
Dye-decolorizing peroxidases (DyPs) from Auricularia auricula-judae, Bjerkandera adusta, Pleurotus ostreatus and Marasmius scorodonius (Basidiomycota) were expressed in Escherichia coli using the cold shock-inducible expression system pCOLD I DNA. Functional expression was achieved without the addition of hemin or the co-expression of any chaperones. The presence or absence of the native signal sequence had a strong impact on the success of the expression, but the effect was not consistent for the different DyPs. While BaDyP and AajDyP were stable at 50 °C, the more thermolabile MsP2 and PoDyp, upon catalytic intervention, lend themselves to more rapid thermal inactivation. The bleaching of norbixin (E 160b) using MsP2 was most efficient at pH 4.0, while BaDyP and AajDypP worked best in the weakly acidic to neutral range, indicating a choice of DyPs for a broad field of applications in different food matrices.
Gottfried Wilhelm Leibniz Universität Hannover, Institut für Lebensmittelchemie, Callinstraße 5, 30167, Hannover, Germany. christoph.behrens@lci.uni-hannover.de.
Christoph J
CJ
Behrens
Gottfried Wilhelm Leibniz Universität Hannover, Institut für Lebensmittelchemie, Callinstraße 5, 30167, Hannover, Germany.
Kateryna
K
Zelena
Gottfried Wilhelm Leibniz Universität Hannover, Institut für Lebensmittelchemie, Callinstraße 5, 30167, Hannover, Germany.
Ralf G
RG
Berger
D000595:Amino Acid Sequence; D052160:Benzothiazoles / Q000378:metabolism; D031212:Bixaceae / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D003080:Cold Temperature; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D005800:Genes, Fungal; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism; D020076:Pleurotus / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000378:metabolism
D052160:Benzothiazoles; D004396:Coloring Agents; D005656:Fungal Proteins; D010936:Plant Extracts; D011994:Recombinant Proteins; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002338:Carotenoids; C054041:annatto; D010544:Peroxidases
Basidiomycota; Dye-decolorizing peroxidase (DyP); Escherichia coli; Heterologous expression