Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells.
This study was designed to determine the effects of receptor activator of nuclear factor κB ligand (RANKL) on the mRNA expression of nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) and c‑Src in rat osteoclast‑like cells. The marrow cells were exposed to macrophage colony-stimulating factor (M‑CSF; 25 ng/ml) and different concentrations of RANKL (0, 50, 75 and 100 ng/ml) for 9 days. The mRNA expression of NFATc1 and c‑Src was determined by polymerase chain reaction. Compared with the M‑CSF (25 ng/ml)+RANKL (0 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression were significantly increased in the M‑CSF (25 ng/ml)+RANKL (75 and 100 ng/ml) groups (P<0.01, P<0.01, P<0.01 and P<0.01, respectively). Compared with the M‑CSF (25 ng/ml)+RANKL (50 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression was significantly increased in the M‑CSF (25 ng/ml)+RANKL (75 and 100 ng/ml) groups (P<0.05, P<0.01, P<0.01 and P<0.01, respectively). Compared with M‑CSF (25 ng/ml)+RANKL (75 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression was significantly increased in the M‑CSF (25 ng/ml)+RANKL (100 ng/ml) group, (P<0.01 and P<0.01, respectively). These data suggest that RANKL could regulate the expression of NFATc1 and c‑Src mRNA in the marrow culture system.
Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, Fujian 361004, P.R. China.
Jifan
J
Fu
Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, Fujian 361004, P.R. China.
You Di
YD
Tao
Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, Fujian 361004, P.R. China.
Jian
J
Chen
Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, Fujian 361004, P.R. China.
Yongsheng
Y
Zhang
Department of Rehabilitation, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, Fujian 361004, P.R. China.
Jianquan
J
He
D000818:Animals; D000081247:CSK Tyrosine-Protein Kinase; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D053245:RANK Ligand / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000378:metabolism*; D019061:src-Family Kinases / Q000096:biosynthesis*
C584440:NFATC1 protein, rat; D053245:RANK Ligand; D014157:Transcription Factors; D000081247:CSK Tyrosine-Protein Kinase; D019061:src-Family Kinases