The effects of different levels of superoxide dismutase in Modena on boar semen quality during liquid preservation at 17°C.
This study was conducted to investigate the influence of superoxide dismutase (SOD) on the quality of boar semen during liquid preservation at 17°C. Semen samples from 10 Duroc boars were collected and pooled, divided into five equal parts and diluted with Modena containing different concentrations (0, 100, 200, 300 and 400 U/mL) of SOD. During the process of liquid preservation at 17°C, sperm motility, acrosome integrity, membrane integrity, total antioxidant capacity (T-AOC) activity, malondialdehyde (MDA) content and hydrogen peroxide (H2O2) content were measured and analyzed every 24 h. Meanwhile, effective survival time of boar semen during preservation was evaluated and analyzed. The results indicated that different concentrations of SOD in Modena showed different protective effects on boar sperm quality. Modena supplemented with SOD decreased the effects on reactive oxygen species on boar sperm quality during liquid preservation compared with that of the control group. The added 200 U/mL SOD group showed higher sperm motility, membrane integrity, acrosome integrity, effective survival time and T-AOC activity. Meanwhile, the added 200 U/mL SOD group showed lower MDA content and H2O2content. In conclusion, addition of SOD to Modena improved the boar sperm quality by reducing oxidative stress during liquid preservation at 17°C and the optimum concentration was 200 U/mL.
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, China.
Xiao-Gang
XG
Zhang
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, China.
Hao
H
Li
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, China.
Le
L
Wang
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, China.
Yang-Yi
YY
Hao
Dali County ZhongKang Livestock Breeding Co., Ltd, Dali Shaanxi, China.
Guo-Dong
GD
Liang
Dali County ZhongKang Livestock Breeding Co., Ltd, Dali Shaanxi, China.
Yun-Hui
YH
Ma
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, China.
Gong-She
GS
Yang
College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, China.
Jian-Hong
JH
Hu
D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology*; D013482:Superoxide Dismutase / Q000494:pharmacology*; D013552:Swine; D013696:Temperature
D000975:Antioxidants; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase
antioxidant capacity; boar semen; liquid preservation; reactive oxygen species; superoxide dismutase