microRNA signatures in peripheral blood fail to detect acute cellular rejection after liver transplantation.
OBJECTIVE
We investigated whether microRNA signatures in whole blood samples are associated with acute cellular rejection (ACR) after liver transplantation.


METHODS
Blood samples were collected using Paxgene technology and analyzed by microarrays and quantitative real-time polymerase chain reaction (qRT-PCR).


RESULTS
microRNA signatures failed to distinguish between 19 patients with ACR and 16 controls. Let-7b-5p and let-7c were upregulated in a subgroup of patients with ACR during the 6th and 7th postoperative days but failed in an independent validation of 20 patients.


CONCLUSIONS
microRNA signatures in whole blood processed by Paxgene technology are not suited for the detection of ACR after liver transplantation.
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Nathanael
N
Raschzok
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Anja
A
Reutzel-Selke
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Rosa B
RB
Schmuck
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Laura M
LM
Tannus
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.|b Department of Visceral, Transplantation, Thoracic, and Vascular Surgery , University of Leipzig , Leipzig , Germany.
Mehmet H
MH
Morgul
b Department of Visceral, Transplantation, Thoracic, and Vascular Surgery , University of Leipzig , Leipzig , Germany.
Corinna
C
Dietel
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Annekatrin
A
Leder
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Benjamin
B
Struecker
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Steffen
S
Lippert
c Department of Neonatology , Charité - Universitätsmedizin Berlin , Germany.
Hannes
H
Sallmon
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.|d Department of Translational Centre for Regenerative Medicine , University of Leipzig , Leipzig , Germany.
Moritz
M
Schmelzle
b Department of Visceral, Transplantation, Thoracic, and Vascular Surgery , University of Leipzig , Leipzig , Germany.
Michael
M
Bartels
e Centre Hépato-Biliaire , Hôpital Paul Brousse, Université Paris Sud , Villejuif , France.
Sven
S
Jonas
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Johann
J
Pratschke
a Department of General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine , Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum , Berlin , Germany.
Igor M
IM
Sauer
Allograft; Paxgene; biomarker