Screening of potential probiotic lactic acid bacteria based on gastrointestinal properties and perfluorooctanoate toxicity.
The consumption of lactic acid bacteria capable of binding or degrading food-borne carcinogens may reduce human exposure to these deleterious compounds. In this study, 25 Lactobacillus strains isolated from human, plant, or dairy environments were investigated for their potential probiotic capacity against perfluorooctanoate (PFOA) toxicity. The PFOA binding, tolerance ability, and acid and bile salt tolerance were investigated and assessed by principal component analysis. Additionally, the effect of different pH levels and binding times was assessed. These strains exhibited different degrees of PFOA binding; the strain with the highest PFOA binding capability was Lactobacillus plantarum CCFM738, which bound to 49.40 ± 1.5 % of available PFOA. This strain also exhibited relatively good cellular antioxidative properties, acid and bile salt tolerance, and adhesion to Caco-2 cells. This study suggests that L. plantarum CCFM738 could be used as a potential probiotic in food applications against PFOA toxicity.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.|Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, 214122, People's Republic of China.
Jiali
J
Xing
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, 210046, People's Republic of China.
Fan
F
Wang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Qi
Q
Xu
Kangyuan Dairy Co., Ltd., Yangzhou University, Yangzhou, 225004, People's Republic of China.
Boxing
B
Yin
Kangyuan Dairy Co., Ltd., Yangzhou University, Yangzhou, 225004, People's Republic of China.
Dongsheng
D
Fang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Jianxin
J
Zhao
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Hao
H
Zhang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Yong Q
YQ
Chen
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. wanggang@jiangnan.edu.cn.|Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, 214122, People's Republic of China. wanggang@jiangnan.edu.cn.
Gang
G
Wang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.|Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, 214122, People's Republic of China.|Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 100048, People's Republic of China.
Wei
W
Chen
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D002210:Caprylates / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D045744:Cell Line, Tumor; D005466:Fluorocarbons / Q000378:metabolism*; D005516:Food Microbiology; D005744:Gastric Acid / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D018384:Oxidative Stress; D025341:Principal Component Analysis; D019936:Probiotics / Q000378:metabolism*; D011485:Protein Binding
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D002210:Caprylates; D002273:Carcinogens; D005466:Fluorocarbons; C023036:perfluorooctanoic acid
Antioxidative activity; Binding capability; Lactic acid bacteria; Perfluorooctanoate; Toxicity