Characterization of embryonic stem cell model of polycystic ovary syndrome.
The aims of this study are to establish an embryonic stem (ES) cell model of polycystic ovary syndrome and to characterize this ES cell line. ES cells were isolated and cultured from 322 wasted fertilized embryos from polycystic ovary syndrome (PCOS) patients in vitro. They were also characterized by development and differential markers. ES cells from PCOS subject present normal development profile with ES-specific markers such as OCT-4 and SSEA-4. These ES cells can also differentiate into three germ layer derivatives and form teratomas in vivo. ES cells from PCOS patients pose development and differentiation potentials as you would expect of cells from non-PCOS patients; therefore, they can be used as a cellular model to study the pathology of PCOS.
Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin, 300052, China.|Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, 300052, China.
Yue
Y
Zhang
Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, 300052, China.
Yun-Shan
YS
Zhang
Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin, 300052, China. tianjinxfx@126.com.
Feng-Xia
FX
Xue
D000328:Adult; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D059785:Karyotype; D011085:Polycystic Ovary Syndrome / Q000473:pathology*
Differentiation; Phenotype; Polycystic ovary syndrome; Stem cell