Facile Assembly of Functional Upconversion Nanoparticles for Targeted Cancer Imaging and Photodynamic Therapy.
The treatment depth of existing photodynamic therapy (PDT) is limited because of the absorption of visible excitation light in biological tissue. It can be augmented by means of upconversion nanoparticles (UCNPs) transforming deep-penetrating near-infrared (NIR) light to visible light, exciting PDT drugs. We report here a facile strategy to assemble such PDT nanocomposites functionalized for cancer targeting, based on coating of the UCNPs with a silica layer encapsulating the Rose Bengal photosensitizer and bioconjugation to antibodies through a bifunctional fusion protein consisting of a solid-binding peptide linker genetically fused to Streptococcus Protein G'. The fusion protein (Linker-Protein G) mediates the functionalization of silica-coated UCNPs with cancer cell antibodies, allowing for specific target recognition and delivery. The resulting nanocomposites were shown to target cancer cells specifically, generate intracellular reactive oxygen species under 980 nm excitation, and induce NIR-triggered phototoxicity to suppress cancer cell growth in vitro.
Liuen
L
Liang
Andrew
A
Care
Run
R
Zhang
Yiqing
Y
Lu
Nicolle H
NH
Packer
Anwar
A
Sunna
Yi
Y
Qian
Laboratory of Optical Theranostics, N. I. Lobachevsky State University of Nizhny Novgorod , Nizhny Novgorod 603950, Russia.
Andrei V
AV
Zvyagin
D000912:Antibodies, Neoplasm / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry / Q000627:therapeutic use; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods*; D012395:Rose Bengal / Q000737:chemistry / Q000494:pharmacology
D000912:Antibodies, Neoplasm; D012395:Rose Bengal
luminescence resonance energy transfer; photodynamic therapy; solid-binding peptides; targeted imaging; upconversion nanoparticles