Disruption of OsSULTR3;3 reduces phytate and phosphorus concentrations and alters the metabolite profile in rice grains.
Two low phytic acid (lpa) mutants have been developed previously with the aim to improve the nutritional value of rice (Oryza sativa) grains. In the present study, the impacts of lpa mutations on grain composition and underlying molecular mechanisms were investigated. Comparative compositional analyses and metabolite profiling demonstrated that concentrations of both phytic acid (PA) and total phosphorus (P) were significantly reduced in lpa brown rice, accompanied by changes in other metabolites and increased concentrations of nutritionally relevant compounds. The lpa mutations modified the expression of a number of genes involved in PA metabolism, as well as in sulfate and phosphate homeostasis and metabolism. Map-based cloning and complementation identified the underlying lpa gene to be OsSULTR3;3. The promoter of OsSULTR3;3 is highly active in the vascular bundles of leaves, stems and seeds, and its protein is localized in the endoplasmic reticulum. No activity of OsSULTR3;3 was revealed for the transport of phosphate, sulfate, inositol or inositol 1,4,5 triphosphate by heterologous expression in either yeast or Xenopus oocytes. The findings reveal that OsSULTR3;3 plays an important role in grain metabolism, pointing to a new route to generate value-added grains in rice and other cereal crops.
State Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou, 310058, China.|Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, 434025, China.
Haijun
H
Zhao
Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Maximum-von-Imhof-Forum 2, Freising-Weihenstephan, D-85354, Germany.
Thomas
T
Frank
State Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou, 310058, China.
Yuanyuan
Y
Tan
Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Maximum-von-Imhof-Forum 2, Freising-Weihenstephan, D-85354, Germany.
Chenguang
C
Zhou
Department of Plant Molecular Biology, Biophore, University of Lausanne, Lausanne, CH-1015, Switzerland.
Mehdi
M
Jabnoune
Department of Plant Molecular Biology, Biophore, University of Lausanne, Lausanne, CH-1015, Switzerland.
A Bulak
AB
Arpat
Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
Hairui
H
Cui
Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
Jianzhong
J
Huang
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Zuhua
Z
He
Department of Plant Molecular Biology, Biophore, University of Lausanne, Lausanne, CH-1015, Switzerland.
Yves
Y
Poirier
Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Maximum-von-Imhof-Forum 2, Freising-Weihenstephan, D-85354, Germany.
Karl-Heinz
KH
Engel
State Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou, 310058, China.|Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, 434025, China.
Qingyao
Q
Shu
D027321:Anion Transport Proteins / Q000378:metabolism*; D001692:Biological Transport; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005966:Glucuronidase / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D010833:Phytic Acid / Q000096:biosynthesis / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D058526:Plant Vascular Bundle / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism
D027321:Anion Transport Proteins; D010940:Plant Proteins; D013431:Sulfates; D010758:Phosphorus; D013455:Sulfur; D010833:Phytic Acid; D005966:Glucuronidase
anti-nutrient; metabolite profiling; phosphate transporter; phosphorus; phytic acid; rice (Oryza sativa); sulfate transporter; γ-aminobutyric acid