A biological process effective for the conversion of CO-containing industrial waste gas to acetate.
Acetogens have often been observed to be inhibited by CO above an inhibition threshold concentration. In this study, a two-stage culture consisting of carboxydotrophic archaea and homoacetogenic bacteria is found to be effective in converting industrial waste gas derived from a steel mill process. In the first stage, Thermococcus onnurineus could grow on the Linz-Donawitz converter gas (LDG) containing ca. 56% CO as a sole energy source, converting the CO into H2 and CO2. Then, in the second stage, Thermoanaerobacter kivui could grow on the off-gas from the first stage culture, consuming the H2 and CO in the off-gas completely and producing acetate as a main product. T. kivui alone could not grow on the LDG gas. This work represents the first demonstration of acetate production using steel mill waste gas by a two-stage culture of carboxydotrophic hydrogenogenic microbes and homoacetogenic bacteria.
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, Republic of Korea.
Tae Wan
TW
Kim
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, Republic of Korea.
Seung Seob
SS
Bae
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Microbiology, Chungbuk National University, Republic of Korea.
Jin Woo
JW
Lee
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea.
Sung-Mok
SM
Lee
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, Republic of Korea.
Jung-Hyun
JH
Lee
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, Republic of Korea. Electronic address: leeh522@kiost.ac.
Hyun Sook
HS
Lee
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, Republic of Korea. Electronic address: sgkang@kiost.ac.
Sung Gyun
SG
Kang
D000085:Acetates / Q000737:chemistry*; D002248:Carbon Monoxide / Q000737:chemistry*; D007220:Industrial Waste; D046968:Thermoanaerobacter / Q000378:metabolism; D019712:Thermococcus / Q000378:metabolism; D018505:Waste Management / Q000379:methods*
D000085:Acetates; D007220:Industrial Waste; D002248:Carbon Monoxide
Gas fermentation; Steel mill waste gas; Thermoanaerobacter kivui; Thermococcus onnurineus; Two-stage culture