Circular RNA expression in basal cell carcinoma.
Circular RNAs (circRNAs), are nonprotein coding RNAs consisting of a circular loop with multiple miRNA, binding sites called miRNA response elements (MREs), functioning as miRNA sponges. This study was performed to identify differentially expressed circRNAs and their MREs in basal cell carcinoma (BCC).



Microarray circRNA expression profiles were acquired from BCC and control followed by qRT-PCR validation. Bioinformatical target prediction revealed multiple MREs. Sequence analysis was performed concerning MRE interaction potential with the BCC miRNome.



We identified 23 upregulated and 48 downregulated circRNAs with 354 miRNA response elements capable of sequestering miRNA target sequences of the BCC miRNome.



The present study describes a variety of circRNAs that are potentially involved in the molecular pathogenesis of BCC.
Dermatologic Surgery Unit, Department of Dermatology, Venereology & Allergology, Ruhr-University Bochum, 44791 Bochum, Germany.|Department of Plastic Surgery, St. Josef Hospital, Catholic Clinics of the Ruhr Peninsula, 45257 Essen, Germany.
Michael
M
Sand
Dermatologic Surgery Unit, Department of Dermatology, Venereology & Allergology, Ruhr-University Bochum, 44791 Bochum, Germany.
Falk G
FG
Bechara
University of Michigan Kellogg Eye Center, Ann Arbor, MI 48105, USA.
Daniel
D
Sand
Dermatologic Surgery Unit, Department of Dermatology, Venereology & Allergology, Ruhr-University Bochum, 44791 Bochum, Germany.
Thilo
T
Gambichler
Department of Internal Medicine, Knappschaftskrankenhaus University of Bochum, Zentrum für Klinische Forschung, Labor für Molekulare Gastroenterologische Onkologie, 44780 Bochum, Germany.
Stephan A
SA
Hahn
Department of Plastic Surgery, St. Josef Hospital, Catholic Clinics of the Ruhr Peninsula, 45257 Essen, Germany.
Michael
M
Bromba
Dermatologic Surgery Unit, Department of Dermatology, Venereology & Allergology, Ruhr-University Bochum, 44791 Bochum, Germany.
Eggert
E
Stockfleth
Dermatologic Surgery Unit, Department of Dermatology, Venereology & Allergology, Ruhr-University Bochum, 44791 Bochum, Germany.
Schapoor
S
Hessam
D002280:Basal Cell Carcinoma / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D006801:Humans; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D000079962:RNA, Circular; D020218:Response Elements; D017423:Sequence Analysis, RNA; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
D000079962:RNA, Circular; D012313:RNA
basal cell carcinoma; circRNAs; circular RNA; noncoding RNAs