Single cell oil production from a newly isolated Candida viswanathii Y-E4 and agro-industrial by-products valorization.
Microbial lipids have drawn increasing attention in recent years as promising raw materials for biodiesel and added-value compounds production. To this end, new oleaginous yeast, Candida viswanathii Y-E4 was isolated, characterized and used for single cell oil (SCO) production. Physiologic and nutritional parameters optimization was carried out for improved biomass and lipid production. Y-E4 strain was able to use a wide range of substrates, especially C5 and C6 sugars as well as glycerol and hydrophobic substrates. The fatty acid profile analysis showed that oleic acid was the main component produced using different substrates. Batch and fed-bath fermentation were conducted using glucose as carbon source. Lipid production rate is twice higher in fed-batch culture providing a lipid content of 50 % (w/w). To minimize the SCO production cost, C. viswanathii Y-E4 was evaluated for its capacity to use different agro-industrial by-products for microbial oil production and changes in the fatty acid profile were monitored.
Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3038, Sfax, Tunisia.
Ines
I
Ayadi
Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3038, Sfax, Tunisia.
Omama
O
Kamoun
Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3038, Sfax, Tunisia.
Hèla
H
Trigui-Lahiani
Laboratory of the National Office Oils, Sfax, Tunisia.
Anouar
A
Hdiji
Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3038, Sfax, Tunisia.
Ali
A
Gargouri
Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3038, Sfax, Tunisia.
Hafedh
H
Belghith
Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3038, Sfax, Tunisia. mguerfali@gmail.com.
Mohamed
M
Guerfali
D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002175:Candida / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D007218:Industrial Microbiology; D008055:Lipids / Q000096:biosynthesis*; D019301:Oleic Acid / Q000032:analysis; D010802:Phylogeny; D059010:Single-Cell Analysis
D003470:Culture Media; D004271:DNA, Fungal; D005227:Fatty Acids; D008055:Lipids; D019301:Oleic Acid
Agro-industrial by-products; Candida viswanathii; Fatty acid; Fed-batch; Single cell oil