Evaluation of Mucor indicus and Saccharomyces cerevisiae capability to ferment hydrolysates of rape straw and Miscanthus giganteus as affected by the pretreatment method.
Rape straw and Miscanthus giganteus was pretreated chemically with oxalic acid or sodium hydroxide. The pretreated substrates were hydrolyzed with enzymatic preparations of cellulase, xylanase and cellobiase. The highest concentration of reducing sugars was achieved after hydrolysis of M. giganteus pretreated with NaOH (51.53gdm(-3)). In turn, the highest yield of enzymatic hydrolysis determined based on polysaccharides content in the pretreated substrates was obtained in the experiments with M. giganteus and oxalic acid (99.3%). Rape straw and M. giganteus hydrolysates were fermented using yeast Saccharomyces cerevisiae 7, NRRL 978 or filamentous fungus Mucor rouxii (Mucor indicus) DSM 1191. The highest ethanol concentration was determined after fermentation of M. giganteus hydrolysate pretreated with NaOH using S. cerevisiae (1.92% v/v). Considering cellulose content in the pretreated solid, the highest degree of its conversion to ethanol (86.2%) was achieved after fermentation of the hydrolysate of acid-treated M. giganteus using S. cerevisiae.
Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland.
Małgorzata
M
Lewandowska
Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland. Electronic address: karolina.swiatek@uwm.edu.pl.
Karolina
K
Szymańska
Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland.
Natalia
N
Kordala
Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Oczapowskiego 7, 10-719 Olsztyn, Poland.
Aneta
A
Dąbrowska
Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland.
Włodzimierz
W
Bednarski
Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Heweliusza 1, 10-718 Olsztyn, Poland.
Andrzej
A
Juszczuk
D001709:Biotechnology / Q000379:methods; D029741:Brassica rapa / Q000737:chemistry / Q000378:metabolism*; D002241:Carbohydrates / Q000737:chemistry; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D009089:Mucor / Q000378:metabolism*; D019815:Oxalic Acid / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D006109:Poaceae / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000737:chemistry; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism
D002241:Carbohydrates; D011134:Polysaccharides; D000431:Ethanol; D012972:Sodium Hydroxide; C007916:hemicellulose; D002482:Cellulose; D019815:Oxalic Acid; D001617:beta-Glucosidase; D002480:Cellulase
Bioethanol; Enzymatic hydrolysis; Fermentation; Lignocellulose; Pretreatment