Effect of various concentrations of butylated hydroxyanisole and butylated hydroxytoluene on freezing capacity of Turkman stallion sperm.
The present study aimed to determine the effect of different concentrations of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on post-thaw stallion sperm quality. The ejaculates collected from four healthy mature Turkmen stallions were pooled and divided into eight aliquots. The samples were diluted with extenders containing different concentrations (0.5, 1 or 2mM/mL) of BHA or BHT. The positive control (PC) samples were diluted with extender containing 0.5% ethanol (v/v) whereas; the negative control (NC) samples were diluted with basic extender only. Semen samples were frozen according to a standard protocol. After thawing of samples, sperm motility, viability, membrane integrity, total abnormality and lipid peroxidation were assessed. The greatest (P<0.05) values for total sperm motility, viability and plasma membrane functionality and least values for malonedialdehyde (MDA) concentration were observed in samples supplemented either with 1mM BHT or 2mM BHA. However, the progressive motility was greater (P<0.05) only in samples treated with 2mM BHA. In conclusion, the use of 1mM BHT or 2mM BHA in extender improves the freezing capacity of stallion sperm by reducing oxidative stress during freeze-thaw process.
Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Afshin
A
Seifi-Jamadi
Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran. Electronic address: Hamid_Kohram@yahoo.com.
Hamid
H
Kohram
Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Ahmad
A
Zareh-Shahne
Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Parvaneh
P
Dehghanizadeh
Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Ejaz
E
Ahmad
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002462:Cell Membrane / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D006736:Horses / Q000502:physiology*; D008297:Male; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
D000975:Antioxidants; D003451:Cryoprotective Agents; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole
BHA; BHT; Freezing; Horse semen; Lipid peroxidation; Sperm motility