Protective effect of bioactive compounds from Lonicera japonica Thunb. against H2O2-induced cytotoxicity using neonatal rat cardiomyocytes.
OBJECTIVE
Pharmacological studies showed that the extracts of Jin Yin Hua and its active constituents have lipid lowering, antipyretic, hepatoprotective, cytoprotective, antimicrobial, antibiotic, antioxidative, antiviral, and anti-inflammatory effects. The purpose of the present study was to investigate the protective effects of caffeoylquinic acids (CQAs) from Jin Yin Hua against hydrogen peroxide (H2O2)-induced and hypoxia-induced cytotoxicity using neonatal rat cardiomyocytes.


METHODS
Seven CQAs (C1 to C7) isolated and identified from Jin Yin Hua were used to examine the effects of H2O2-induced and hypoxia-induced cytotoxicity. We studied C4 and C6 as preventative bioactive compounds of the reactive oxygen species (ROS) production, apoptotic pathway, and apoptosis-related gene expression.


RESULTS
C4 and C6 were screened as bioactive compounds to exert a cytoprotective effect against oxidative injury. Pretreatment with C4 and C6, dose-dependently attenuated hypoxia-induced ROS production and reduced the ratio of GSSG/GStotal. Western blot data revealed that the inhibitory effect of C4 on H2O2-induced up and down-regulation of Bcl-2, Bax, caspase-3, and cleaved caspase-3. Apoptosis was evaluated by detection of DNA fragmentation using TUNEL assay, and quantified with Annexin V/PI staining.


CONCLUSIONS
In vitro experiments revealed that both C4 and C6 protect cardiomyocytes from necrosis and apoptosis during H2O2-induced injury, via inhibiting the generation of ROS and activation of caspase-3 apoptotic pathway. These results demonstrated that CQAs might be a class of compounds which possess potent myocardial protective activity against the ischemic heart diseases related to oxidative stress.
Department of Medical market, Cangzhou Central Hospital, Hebei Province, 061001, China.
Chen
C
Wang
Department of Cardiology, Cangzhou Cardiovascular Research Institute, Cangzhou Central Hospital, Hebei Province, 061001, China.
Gang
G
Wang
Department of Pharmacy, General Hospital of Jixi Mining Industry Group, Heilongjiang Province, 158100, China.
Hong
H
Liu
Department of Pharmacy, Harbin Medical University, Heilongjiang Province, 150086, China.
Yun-Long
YL
Hou
Anti-apoptosis; Caffeoylquinic acids; Cardiomyocytes; Lonicera japonica Thunb; Oxidative stress