MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection.
Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is an important swine disease worldwide. PRRSV has a limited tropism for certain cells, which may at least in part be attributed to the expression of the necessary cellular molecules serving as the virus receptors or factors on host cells for virus binding or entry. However, these molecules conferring PRRSV infection have not been fully characterized. Here we show the identification of non-muscle myosin heavy chain 9 (MYH9) as an essential factor for PRRSV infection using the anti-idiotypic antibody specific to the PRRSV glycoprotein GP5. MYH9 physically interacts with the PRRSV GP5 protein via its C-terminal domain and confers susceptibility of cells to PRRSV infection. These findings indicate that MYH9 is an essential factor for PRRSV infection and provide new insights into PRRSV-host interactions and viral entry, potentially facilitating development of control strategies for this important swine disease.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Jiming
J
Gao
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Shuqi
S
Xiao
Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, China.
Yihong
Y
Xiao
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Xiangpeng
X
Wang
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Chong
C
Zhang
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Qin
Q
Zhao
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Yuchen
Y
Nan
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Baicheng
B
Huang
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Hongliang
H
Liu
Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, China.
Ningning
N
Liu
Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, China.
Junhua
J
Lv
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Taofeng
T
Du
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Yani
Y
Sun
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Yang
Y
Mu
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agriculture Science, Harbin 150001, China.
Gang
G
Wang
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
Shahid Faraz
SF
Syed
Department of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China.
Gaiping
G
Zhang
Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, United Kingdom.
Julian A
JA
Hiscox
Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom.
Ian
I
Goodfellow
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
En-Min
EM
Zhou
D000818:Animals; D002460:Cell Line; D054884:Host-Pathogen Interactions; D024507:Nonmuscle Myosin Type IIA / Q000378:metabolism*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D013552:Swine; D014759:Viral Envelope Proteins / Q000378:metabolism*
D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV; D024507:Nonmuscle Myosin Type IIA