Role of MicroRNA 126 in screening, diagnosis, and prognosis of diabetic patients in Egypt.
MicroRNAs (miRNAs), family of non-coding small RNAs, play a vital role in the regulation of blood glucose level. We aimed to investigate the relation of serum miRNA-126 expression with impaired glucose tolerance as well as type 2 diabetes mellitus (T2DM) patients with and without complications. One hundred healthy controls, eighty-six patients with IGT, and one hundred patients with T2DM were recruited in this study. Serum miRNA-126 expression was assessed by quantitative real-time polymerase chain reaction. We found a significant decrease of serum miRNA-126 expression between IGT patients as well as diabetic patients when both compared with controls and between diabetic patients compared to IGT patients. A significant decrease of serum miRNA-126 expression was detected in diabetic patients with complications compared to those without evident complications especially those with diabetic macrovascular complications and diabetic retinopathy. Serum microRNA-126 expression could be a good marker for diagnosis of IGT and T2DM as well as for monitoring the outcomes of such disease. © 2016 IUBMB Life, 68(6):452-458, 2016.
Faculty of Medicine, Medical Biochemistry Department, Zagazig University, Egypt.
Noha A
NA
Rezk
Faculty of Medicine, Medical Biochemistry Department, Zagazig University, Egypt.
Norhan A
NA
Sabbah
Faculty of Medicine, Internal Medicine Department, Zagazig University, Egypt.
Mohamed S S
MS
Saad
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D048909:Diabetes Complications / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*; D004534:Egypt; D005260:Female; D018149:Glucose Intolerance / Q000097:blood / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011379:Prognosis
D015415:Biomarkers; C527111:MIRN126 microRNA, human; D035683:MicroRNAs
IGT; MicroRNA 126; complications; diabetes mellitus; qRT-PCR