Near-Infrared Illumination of Native Tissues for Image-Guided Surgery.
Our initial efforts to prepare tissue-specific near-infrared (NIR) fluorescent compounds generated successful correlation between physicochemical properties and global uptake in major organs after systemic circulation and biodistribution. Herein, we focus on the effects on biodistribution based on modulating electronic influencing moieties from donating to withdrawing moieties at both the heterocyclic site and through meso-substitution of pentamethine cyanine fluorophores. These selected modifications harnessed innate biodistribution pathways through the structure-inherent targeting, resulting in effective imaging of the adrenal glands, pituitary gland, lymph nodes, pancreas, and thyroid and salivary glands. These native-tissue contrast agents will arm surgeons with a powerful and versatile arsenal for intraoperative NIR imaging in real time.
Center for Diagnostics and Therapeutics, Center for Biotechnology and Drug Design, Department of Chemistry, Georgia State University , Petit Science Center, 100 Piedmont Ave SE, Atlanta, Georgia 30303, United States.
Eric A
EA
Owens
Gordon Center for Medical Imaging, Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts 02114, United States.
Hoon
H
Hyun
Center for Diagnostics and Therapeutics, Center for Biotechnology and Drug Design, Department of Chemistry, Georgia State University , Petit Science Center, 100 Piedmont Ave SE, Atlanta, Georgia 30303, United States.
Tyler L
TL
Dost
Gordon Center for Medical Imaging, Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts 02114, United States.
Jeong Heon
JH
Lee
Gordon Center for Medical Imaging, Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts 02114, United States.
GwangLi
G
Park
Dang Huan
DH
Pham
Min Ho
MH
Park
Gordon Center for Medical Imaging, Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts 02114, United States.
Hak Soo
HS
Choi
Center for Diagnostics and Therapeutics, Center for Biotechnology and Drug Design, Department of Chemistry, Georgia State University , Petit Science Center, 100 Piedmont Ave SE, Atlanta, Georgia 30303, United States.
Maged
M
Henary
D000311:Adrenal Glands / Q000000981:diagnostic imaging / Q000601:surgery; D000818:Animals; D002232:Carbocyanines / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D008198:Lymph Nodes / Q000000981:diagnostic imaging / Q000601:surgery; D008297:Male; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D010179:Pancreas / Q000000981:diagnostic imaging / Q000601:surgery; D010902:Pituitary Gland / Q000000981:diagnostic imaging / Q000601:surgery; D012469:Salivary Glands / Q000000981:diagnostic imaging / Q000601:surgery; D025321:Surgery, Computer-Assisted; D013961:Thyroid Gland / Q000000981:diagnostic imaging / Q000601:surgery; D014018:Tissue Distribution
D002232:Carbocyanines; D005456:Fluorescent Dyes