Elucidating Structural Characteristics of Biomass using Solution-State 2 D NMR with a Mixture of Deuterated Dimethylsulfoxide and Hexamethylphosphoramide.
Recent developments of NMR methods for characterization of lignocellulosic biomass allow improved understanding of plant cell-wall structures with minimal deconstruction and modification of biomass. This study introduces a new NMR solvent system composed of dimethylsulfoxide (DMSO-d6 ) and hexamethylphosphoramide (HMPA-d18 ). HMPA as a co-solvent enhanced swelling and mobility of the biomass samples; thereby it allowed enhancing signals of NMR spectra. The structural information of biomass was successfully analyzed by the proposed NMR solvent system (DMSO-d6 /HMPA-d18 ; 4:1, v/v) with different biomass. The proposed bi-solvent system does not require derivatization or isolation of biomass, facilitating a facile sample preparation and involving with no signals overlapping with biomass peaks. It also allows analyzing biomass with a room-temperature NMR probe instead of cryo-probes, which are traditionally used for enhancing signal intensities.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.|BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.|UT-ORNL Joint Institute for Biological Science, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.
Chang Geun
CG
Yoo
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.|BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.|UT-ORNL Joint Institute for Biological Science, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.
Yunqiao
Y
Pu
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.|BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.|UT-ORNL Joint Institute for Biological Science, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.
Mi
M
Li
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA. aragauskas@utk.edu.|BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA. aragauskas@utk.edu.|UT-ORNL Joint Institute for Biological Science, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA. aragauskas@utk.edu.|Department of Chemical and Biomolecular Engineering & Department of Forestry, Wildlife, and Fisheries, University of Tennessee, Knoxville, TN, 37996, USA. aragauskas@utk.edu.
Arthur J
0000-0003-1420-6678
AJ
Ragauskas
D018533:Biomass; D003903:Deuterium / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D006492:Hempa / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D012997:Solvents / Q000737:chemistry
D012997:Solvents; D003903:Deuterium; D006492:Hempa; D004121:Dimethyl Sulfoxide
NMR spectroscopy; biomass; characterization; hexamethylphosphoramide; whole cell wall