Evaluation of the inflammatory potential of implant materials in a mouse model by bioluminescent imaging of intravenously injected bone marrow cells.
To evaluate the inflammatory potential of implants a bioluminescent imaging assay was developed using luciferase-expressing bone marrow cells that were injected into the blood circulation of wild-type mice. After subcutaneous implantation of titanium discs as an example for a clinically established biocompatible material, the luminosity was modest. Similarly, low luminosity signals were generated by pure magnesium implants that were used to represent metallic alloys that are presently under investigation as novel degradable implant materials. Increased luminosity was observed in response to degradable polymeric PLGA implants. Surgical wounds induced a basic luminescent response even in the absence of an implant. However, the material-independent response to injury could be minimized using injectable microparticle suspensions. In parallel with the resorption of biodegradable microparticles, the signal induced by PLGA declined faster when compared to non-degradable polystyrene suspensions. By using an interferon type I inducible Mx2 promoter construct to drive luciferase gene expression, the highest luminosity was observed in response to bacteria, indicating that the system could also be employed to monitor implant infections. Overall, labeled bone marrow cells yielded specific, well-defined localized signals that correlated with the inflammatory responses to implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2149-2158, 2016.
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 38124, Germany.
Bushra
B
Rais
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 38124, Germany.
Mario
M
Köster
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 38124, Germany.
Muhammad Imran
MI
Rahim
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 38124, Germany.
Marina
M
Pils
Institute of Material Sciences, Leibniz University Hannover, An der Universität 2, Garbsen, 30823, Germany.|Department of Materials Science and Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan, 49931.
Jan-Marten
JM
Seitz
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 38124, Germany.
Hansjörg
H
Hauser
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 38124, Germany.
Dagmar
D
Wirth
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 38124, Germany.
Peter P
PP
Mueller
D020341:Absorbable Implants; D064591:Allografts; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D016026:Bone Marrow Transplantation; D058948:Cell Tracking / Q000379:methods*; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008954:Models, Biological; D011137:Polystyrenes / Q000009:adverse effects / Q000494:pharmacology
D011137:Polystyrenes
bioluminescent imaging; bone marrow cells; implant material; inflammation; mouse model