Culture medium pH influence on Gluconacetobacter physiology: Cellulose production rate and yield enhancement in presence of multiple carbon sources.
Gluconacetobacter genera are valued for bacterial cellulose (BC) and acetic acid production. BC is produced at optimal yields in classical microbiological media that are expensive for a large scale of production. In addition, BC usage for industrial purposes is limited due to low conversion rate into cellulose and to long incubation duration. In this paper, Gluconacetobacter isolated from apple vinegar was kinetically studied to evaluate cellulose production in presence of different carbon sources. Acetic and citric acid effect on Gluconacetobacter metabolism is clarified. It was shown that Gluconacetobacter uses glucose as a primary carbon source for cells growth and products formation. Acetic acid employment as a co-carbon source in Hestrin Schramm medium showed an increase of 17% in BC yield with a moderate decrease in the crystallite size of the resulting polymer.
LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon; PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon; Lyon University, Lyon1 University, IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Villeurbanne, France; Laboratory of Microbiology, Department of Biology, Lebanese University, Faculty of Sciences I, PO Box 5, Hadat, Lebanon. Electronic address: fatima.yassine89@yahoo.com.
Fatima
F
Yassine
LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon.
Nathalie
N
Bassil
LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon.
Roula
R
Flouty
PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon; Laboratory of Microbiology, Department of Biology, Lebanese University, Faculty of Sciences I, PO Box 5, Hadat, Lebanon.
Ali
A
Chokr
PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon.
Antoine El
AE
Samrani
Lyon University, Lyon1 University, IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Villeurbanne, France.
Gisèle
G
Boiteux
LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon; PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon.
Mario El
ME
Tahchi
D002244:Carbon / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D041821:Gluconacetobacter / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology
D003470:Culture Media; D002244:Carbon; D002482:Cellulose
Acetic acid; Carbon source; Cellulosic biofilm; Conversion rate; Gluconacetobacter; pH