Gallium plasmonic nanoparticles for label-free DNA and single nucleotide polymorphism sensing.
A label-free DNA and single nucleotide polymorphism (SNP) sensing method is described. It is based on the use of the pseudodielectric function of gallium plasmonic nanoparticles (GaNPs) deposited on Si (100) substrates under reversal of the polarization handedness condition. Under this condition, the pseudodielectric function is extremely sensitive to changes in the surrounding medium of the nanoparticle surface providing an excellent sensing platform competitive to conventional surface plasmon resonance. DNA sensing has been carried out by immobilizing a thiolated capture probe sequence from Helicobacter pylori onto GaNP/Si substrates; complementary target sequences of Helicobacter pylori can be quantified over the range of 10 pM to 3.0 nM with a detection limit of 6.0 pM and a linear correlation coefficient of R(2) = 0.990. The selectivity of the device allows the detection of a single nucleotide polymorphism (SNP) in a specific sequence of Helicobacter pylori, without the need for a hybridization suppressor in solution such as formamide. Furthermore, it also allows the detection of this sequence in the presence of other pathogens, such as Escherichia coli in the sample. The broad applicability of the system was demonstrated by the detection of a specific gene mutation directly associated with cystic fibrosis in large genomic DNA isolated from blood cells.
Grupo de Electrónica y Semiconductores, Departamento de Física Aplicada, Universidad Autónoma de Madrid, Spain.
Antonio García
AG
Marín
Departamento Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, Spain. encarnacion.lorenzo@uam.es and Instituto Madrileño de Estudios Avanzados (IMDEA) Nanociencia, Spain.
Tania
T
García-Mendiola
Instituto Madrileño de Estudios Avanzados (IMDEA) Nanociencia, Spain.
Cristina Navio
CN
Bernabeu
Grupo de Electrónica y Semiconductores, Departamento de Física Aplicada, Universidad Autónoma de Madrid, Spain.
María Jesús
MJ
Hernández
Grupo de Electrónica y Semiconductores, Departamento de Física Aplicada, Universidad Autónoma de Madrid, Spain.
Juan
J
Piqueras
Grupo de Electrónica y Semiconductores, Departamento de Física Aplicada, Universidad Autónoma de Madrid, Spain.
Jose Luis
JL
Pau
Departamento Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, Spain. encarnacion.lorenzo@uam.es and Instituto Madrileño de Estudios Avanzados (IMDEA) Nanociencia, Spain.
Félix
F
Pariente
Departamento Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, Spain. encarnacion.lorenzo@uam.es and Instituto Madrileño de Estudios Avanzados (IMDEA) Nanociencia, Spain.
Encarnación
E
Lorenzo
D015374:Biosensing Techniques; D003550:Cystic Fibrosis / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D004252:DNA Mutational Analysis; D004912:Erythrocytes; D004926:Escherichia coli; D005708:Gallium; D016480:Helicobacter pylori; D006801:Humans; D053758:Nanoparticles; D020641:Polymorphism, Single Nucleotide
D004247:DNA; D005708:Gallium