Antioxidant and Anti-quorum Sensing Potential of Acer monspessulanum subsp. monspessulanum Extracts.
In this study, anti-quorum sensing, and antioxidant activities, and chemical composition ofAcer monspessulanumsubsp.monspessulanumextracts were evaluated. Determination of the antioxidant activity was revealed by DPPH radical scavenging activity, the total phenolic content assay, and theβ-carotene/linoleic acid assay. The detection of phenolic compounds was determined using RP-HPLC. Anti-quorum sensing activity and violacein inhibition activity were determined usingChromobacterium violaceumCV026 andC. violaceumATCC 112 472, respectively. The determination of anti-swarming activity was carried out withPseudomonas aeruginosaPA01. In DPPH and total phenolic content assays, the water extract exhibited good antioxidant activity. In theβ-carotene-linoleic acid assay, ethyl acetate and ethanol extracts exhibited good lipid peroxidation inhibition activity, demonstrating 96.95 ± 0.03 % and 95.35 ± 0.00 % at 2.5 mg/mL concentrations, respectively. The predominant phenolic compounds of the extracts were determined as rutin, naringin, catechin hydrate, quercetin, and protocatechuic acid. Ethyl acetate and ethanol extracts were found to contain a high level of violacein inhibition and anti-quorum sensing activity. The ethanol extract also showed weak anti-swarming activity. In this first study that usedAcer monspessulanumsubsp.monspessulanumextracts, it was revealed that the water extract has antioxidant activity and the ethanol and ethyl acetate extracts have anti-quorum sensing activity depending on the phenolic compounds that it contained.
Ula Ali Kocman Vocational School, University of Mugla Sıtkı Koçman, Mugla, Turkey.
Ozgur
O
Ceylan
Faculty of Education, University of Usak, Usak, Turkey.
Mehtap Donmez
MD
Sahin
Faculty of Sciences, University of Mugla Sitki Kocman, Mugla, Turkey.
Gultekin
G
Akdamar
D031002:Acer / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002861:Chromobacterium / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007211:Indoles / Q000378:metabolism; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D053038:Quorum Sensing / Q000187:drug effects*; D012431:Rutin / Q000032:analysis
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D007211:Indoles; D010936:Plant Extracts; D012431:Rutin; C063155:violacein