Detection of Cell Death in Drosophila Tissues.
Drosophila has served as a particularly attractive model to study cell death due to the vast array of tools for genetic manipulation under defined spatial and temporal conditions in vivo as well as in cultured cells. These genetic methods have been well supplemented by enzymatic assays and a panel of antibodies recognizing cell death markers. This chapter discusses reporters, mutants, and assays used by various laboratories to study cell death in the context of development and in response to external insults.
Department of Cell Biology, New York University School of Medicine, 550 First Ave., New York, NY, 10016, USA.
Deepika
D
Vasudevan
Department of Cell Biology, New York University School of Medicine, 550 First Ave., New York, NY, 10016, USA. hyungdon.ryoo@nyumc.org.
Hyung Don
HD
Ryoo
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D004330:Drosophila / Q000166:cytology* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics; D005123:Eye / Q000378:metabolism; D005786:Gene Expression Regulation
D029721:Drosophila Proteins; D020169:Caspases
Acridine orange; DEVD cleavage assay; Drosophila cell death reporters; Immunostaining; TUNEL