Computational Design of Protein Linkers.
Naturally occurring proteins often consist of multiple distinct domains joined by linker regions. Similarly, the ability to combine globular protein domains through engineered linkers would allow the creation of a wide variety of complex and useful multifunctional proteins. Recent advances in computational design of protein structures have enabled highly accurate design of novel protein structures. In this chapter we outline a computational protocol for the de novo design of protein linkers, and apply this protocol to the design of a helical linker between two rigid protein domains.
Department of Biochemistry and Biophysics, University of North Carolina, 120 Mason Farm Rd, Campus Box 7260, Chapel Hill, NC, 27599, USA. bkuhlman@email.unc.edu.
Brian
B
Kuhlman
University of North Carolina, Chapel Hill, NC, USA.
Tim
T
Jacobs
University of Washington, Seattle, WA, USA.
Tom
T
Linskey
D001665:Binding Sites; D019295:Computational Biology; D062105:Molecular Docking Simulation; D011506:Proteins / Q000737:chemistry*
D011506:Proteins
Molecular docking; Protein linkers; RosettaDesign