Implementation and Evaluation of a Fully Automated Multiplex Real-Time PCR Assay on the BD Max Platform to Detect and Differentiate Herpesviridae from Cerebrospinal Fluids.
A fully automated multiplex real-time PCR assay--including a sample process control and a plasmid based positive control--for the detection and differentiation of herpes simplex virus 1 (HSV1), herpes simplex virus 2 (HSV2) and varicella-zoster virus (VZV) from cerebrospinal fluids (CSF) was developed on the BD Max platform. Performance was compared to an established accredited multiplex real time PCR protocol utilizing the easyMAG and the LightCycler 480/II, both very common devices in viral molecular diagnostics. For clinical validation, 123 CSF specimens and 40 reference samples from national interlaboratory comparisons were examined with both methods, resulting in 97.6% and 100% concordance for CSF and reference samples, respectively. Utilizing the BD Max platform revealed sensitivities of 173 (CI 95%, 88-258) copies/ml for HSV1, 171 (CI 95%, 148-194) copies/ml for HSV2 and 84 (CI 95%, 5-163) copies/ml for VZV. Cross reactivity could be excluded by checking 25 common viral, bacterial and fungal human pathogens. Workflow analyses displayed shorter test duration as well as remarkable fewer and easier preparation steps with the potential to reduce error rates occurring when manually assessing patient samples. This protocol allows for a fully automated PCR assay on the BD Max platform for the simultaneously detection of herpesviridae from CSF specimens. Singular or multiple infections due to HSV1, HSV2 and VZV can reliably be differentiated with good sensitivities. Control parameters are included within the assay, thereby rendering its suitability for current quality management requirements.
Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.
Thomas
T
Köller
Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.
Daniel
D
Kurze
Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.
Mirjam
M
Lange
Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.
Martin
M
Scherdin
Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.
Andreas
A
Podbielski
Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.
Philipp
P
Warnke
D001681:Biological Assay / Q000379:methods; D002555:Cerebrospinal Fluid / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D006564:Herpesviridae / Q000235:genetics*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004279:DNA, Viral