Evaluation of polypropylene mesh coated with biological hydrogels for temporary closure of open abdomen.
Polypropylene mesh, as a temporary abdominal closure device, may cause mechanical intestine injury and inflammatory response. Chitosan/gelatin hydrogel has excellent biocompatibility, soft and elastic properties. This work is to assess the effects of the chitosan/gelatin hydrogel coated polypropylene mesh on open abdomen wounds. Histological analysis and detection of healing-related factors were conducted to evaluate the inflammation and wound healing process. After 1-day implantation in a murine model of open abdomen, the coated polypropylene mesh, compared with simple polypropylene mesh, demonstrated well protection of the intestine serosa. After 14-day implantation, it reduced the inflammation response by down-regulating the cytokines interleukin-6 and tumor necrosis factor-α, and up-regulating the anti-inflammatory factor interleukin-10. Meanwhile, the composite stimulated granulation tissue growth, and promoted matrix deposition and angiogenesis after 7 and 14 days. In conclusion, the modified temporary abdominal closure composite could significantly protect the intestines from mechanical damage and accelerate wound healing.
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Youming
Y
Deng
Department of General Surgery, Medical School of Nanjing University, People's Republic of China JiananR@gmail.com.
Jianan
J
Ren
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Guopu
G
Chen
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Guanwei
G
Li
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Kun
K
Guo
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Qiongyuan
Q
Hu
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Xiuwen
X
Wu
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Gefei
G
Wang
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Guosheng
G
Gu
Department of General Surgery, Medical School of Nanjing University, People's Republic of China.
Jieshou
J
Li
D058107:Abdominal Wound Closure Techniques; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D048271:Chitosan / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000627:therapeutic use*; D042783:Endothelial Cells / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry; D006097:Granulation Tissue / Q000187:drug effects; D020100:Hydrogels / Q000627:therapeutic use*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D011126:Polypropylenes / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013526:Surgical Mesh; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing
D000893:Anti-Inflammatory Agents; D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D015850:Interleukin-6; D011126:Polypropylenes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D005780:Gelatin; D048271:Chitosan
Polypropylene mesh; chitosan/gelatin hydrogel; granulation tissue; open abdomen; wound healing