New artificial fluoro-cofactor of hydride transfer with novel fluorescence assay for redox biocatalysis.
A new artificial fluoro-cofactor was developed for the replacement of natural cofactors NAD(P), exhibiting a high hydride transfer ability. More importantly, we established a new and fast screening method for the evaluation of the properties of artificial cofactors based on the fluorescence assay and visible color change.
Shanghai Key Laboratory of Chemical Biology, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. yfxu@ecust.edu.cn.
Lei
L
Zhang
Jun
J
Yuan
Yufang
Y
Xu
Y-H Percival
YH
Zhang
Xuhong
X
Qian
D055162:Biocatalysis; D005453:Fluorescence; D015394:Molecular Structure; D009249:NADP / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction
D009249:NADP