Identification of a Bitter-Taste Receptor Gene Repertoire in Different Lagomorphs Species.
The repertoires of bitter-taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, L. europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi, and Sylvilagus floridanus, using O. cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of O. cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in R. diazi and S. floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification.
Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de ÉvoraÉvora, Portugal; Laboratório de Biotecnologia de Células Vegetais, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de LisboaOeiras, Portugal.
Ana M
AM
Ferreira
Dipartimento di Scienze Veterinarie e Sanità Pubblica, Università degli Studi di Milano Milan, Italy.
Andreia T
AT
Marques
Department of Agricultural and Food Sciences, Division of Animal Sciences, University of Bologna Bologna, Italy.
Luca
L
Fontanesi
Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences Umeå, Sweden.
Carl-Gustaf
CG
Thulin
Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de ÉvoraÉvora, Portugal; Departamento de Zootecnia, Universidade de ÉvoraÉvora, Portugal.
Elvira
E
Sales-Baptista
Laboratório de Biotecnologia de Células Vegetais, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de LisboaOeiras, Portugal; Plant Biotechnology Laboratory, Department of Biology and Biotechnology "L. Spallanzani", Università degli Studi di PaviaPavia, Italy.
Susana S
SS
Araújo
Ross University School of Veterinary Medicine, Basseterre Saint Kitts and Nevis.
André M
AM
Almeida
Lagomorph; Lepus; Oryctolagus; Romerolagus; Sylvilagus; TAS2R; bitter-taste receptors