Supramolecular biosensors based on electropolymerised pyrrole-cyclodextrin modified surfaces for antibody detection.
The self-assembly of an adamantane-appended polymer bearing an antigen fragment on a polypyrrole-cyclodextrin modified surface provides a highly sensitive immunosensor with low limits of detection for celiac disease related targets. The pyrrole-carboxylic acid films were formed on the surface of gold electrodes by electropolymerisation and followed by covalent attachment of cyclodextrin units. Surface plasmon resonance measurements confirmed the role of the host/guest interactions between adamantane moieties and β-cyclodextrin hosts in the formation of the supramolecular sensor interface. Furthermore, this novel electrochemical supramolecular platform was effective in the amperometric detection of anti-gliadin antibodies in spiked serum samples with very good signal recovery.
Nanobiotechnology & Bioanalysis Group, Department d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007 Tarragona, Spain. alex.fragoso@urv.cat.
Ewelina
E
Wajs
Núria
N
Fernández
Alex
A
Fragoso
D000906:Antibodies / Q000302:isolation & purification*; D015374:Biosensing Techniques; D002446:Celiac Disease; D003505:Cyclodextrins / Q000737:chemistry*; D006046:Gold; D006801:Humans; D011758:Pyrroles / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D000906:Antibodies; D003505:Cyclodextrins; D011758:Pyrroles; D006046:Gold