A critical role for topoisomerase IIb and DNA double strand breaks in transcription.
Recent studies have indicated a novel role for topoisomerase IIb in transcription. Transcription of heat shock genes, serum-induced immediate early genes and nuclear receptor-activated genes, each required DNA double strands generated by topoisomerase IIb. Such strand breaks seemed both necessary and sufficient for transcriptional activation. In addition, such transcription was associated with initiation of the DNA damage response pathways, including the activation of the enzymes: ataxia-telangiectasia mutated (ATM), DNA-dependent protein kinase and poly (ADP ribose) polymerase 1. DNA damage response signaling was involved both in transcription and in repair of DNA breaks generated by topoisomerase IIb.
a Department of Radiation Oncology , Harvard Medical School at Beth Israel Deaconess Medical Center , Boston , MA , USA.
Stuart K
SK
Calderwood
D004247:DNA / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D045643:DNA Repair Enzymes / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D004247:DNA; D004250:DNA Topoisomerases, Type II; D045643:DNA Repair Enzymes
ATM; DNA-PK; PARP1; PIKK kinase; strand break; topoisomerase II; transcripton