Reversible Gel-Sol Transition of a Photo-Responsive DNA Gel.
Stimuli-responsive DNA gels that can undergo a sol-gel transition in response to photo-irradiation provide a way to engineer functional gel material with fully designed DNA base sequences. We propose an X-shaped DNA motif that turns into a gel by hybridization of self-complementary sticky ends. By embedding a photo-crosslinking artificial base in the sticky-end sequence, repetitive gel-sol transitions are achieved through UV irradiation at different wavelengths. The concentration of the DNA motif necessary for gelation is as low as 40 μm after modification of the geometrical properties of the motif. The physical properties, such as swelling degree and diffusion coefficient, were assessed experimentally.
Department of Robotics, Tohoku University, Aoba, Sendai, Miyagi, 980-8579, Japan.
Daisuke
D
Kandatsu
Department of Robotics, Tohoku University, Aoba, Sendai, Miyagi, 980-8579, Japan.
Keitel
K
Cervantes-Salguero
Department of Robotics, Tohoku University, Aoba, Sendai, Miyagi, 980-8579, Japan.
Ibuki
I
Kawamata
Kavli Institute at Cornell for Nanoscale Science, Cornell University, Ithaca, NY, 14853, USA.
Shogo
S
Hamada
Department of Robotics, Tohoku University, Aoba, Sendai, Miyagi, 980-8579, Japan.
Shin-Ichiro M
SM
Nomura
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, 923-1292, Japan.
Kenzo
K
Fujimoto
Department of Robotics, Tohoku University, Aoba, Sendai, Miyagi, 980-8579, Japan. murata@molbot.mech.tohoku.ac.jp.
Satoshi
S
Murata
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D005782:Gels / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000528:radiation effects; D044367:Phase Transition; D014466:Ultraviolet Rays; D014508:Urea / Q000737:chemistry
D004277:DNA, Single-Stranded; D005782:Gels; D014508:Urea; D004247:DNA
DNA; DNA structures; gels; nanotechnology; photo-responsive DNA