[Research on effect of Sargentodoxae caulis on activity of osteoclasts and proliferation differentiation of osteoblasts].
Through morphological observation, HE staining, TRAP staining and toluidine blue staining of bone resorption pits to identify osteoclasts which obtained by 1α, 25-(OH)2 VitD3 inducing rabbit bone marrow cells. Three indicators-TRAP staining, TRAP enzyme activity detecting and the number and area of bone resorption pits were adapted to detect the effect of Sargentodoxae caulis on the activity of osteoclasts. Culturing MC3T3-E1 Subclong 14 cells and detecting the effect of S. caulis on differentiation and proliferation of them by MTT and detecting the alkaline phosphatase in cells. The results show that all of the low, middle and high doses of water and alcohol extracts of S. caulis have significant inhibition on osteoclast differentiation and bone resorption ability in a dose-dependent manner. The low and middle doses of water and alcohol extracts of S. caulis can stimulate differentiation and proliferation of MC3T3-ElSubclone 14 cells, which indicates S. caulis can prevent osteoporosis and the function could be achieved by inhibiting osteoclast activity and promoting the proliferation and differentiation of osteoblasts.
Li-zhen
LZ
Chen
Ying
Y
Zhou
Jun-fei
JF
Huang
Xue
X
Zhang
Ting-ting
TT
Feng
D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy / Q000503:physiopathology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects*; D011817:Rabbits
D004365:Drugs, Chinese Herbal