miR-136 suppresses tumor invasion and metastasis by targeting RASAL2 in triple-negative breast cancer.
MicroRNAs play an important role in the regulation of cancer migration, invasion and metastasis. Patients with triple-negative breast cancer (TNBC) have a high incidence of early relapse and metastasis; however, the molecular basis for metastasis and recurrence in these individuals remains largely unknown. Herein, we demonstrate that miR-136 is an anti-invasive microRNA in TNBC and suppresses mesenchymal invasion and metastasis. Our results demonstrated that miR-136 was downregulated in TNBC and negative correlated with the WHO grades. However, RASAL2 was identified as a functional target of miR-136, and was overexpressed in TNBC and correlates with pathological grades. Moreover, overexpression of RASAL2 in a breast cancer cell line rescued miR-136-mediated cell migration and invasion. In conclusion, these results indicate that the miR-136/RASAL2/MET axis act as a suppressor of TNBC metastasis.
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Meisi
M
Yan
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Xiaobo
X
Li
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Dandan
D
Tong
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Changsong
C
Han
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Ran
R
Zhao
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Yan
Y
He
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
Xiaoming
X
Jin
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D020690:GTPase-Activating Proteins; D016147:Genes, Tumor Suppressor; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000473:pathology; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D000071250:Snail Family Transcription Factors / Q000096:biosynthesis; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D014746:Vimentin / Q000096:biosynthesis
D015820:Cadherins; D002352:Carrier Proteins; D020690:GTPase-Activating Proteins; C577037:MIRN136 microRNA, human; D035683:MicroRNAs; C576391:RASAL2 protein, human; D000071250:Snail Family Transcription Factors; D014746:Vimentin