Analysis of basic leucine zipper genes and their expression during bud dormancy in peach (Prunus persica).
Dormancy is a biological characteristic developed to resist the cold conditions in winter. The bZIP transcription factors are present exclusively in eukaryotes and have been identified and classified in many species. bZIP proteins are known to regulate numerous biological processes, however, the role of bZIP in bud dodormancy has not been studied extensively. In total, 50 PpbZIP transcription factor-encoding genes were identified and categorized them into 10 groups (A-I and S). Similar intron/exon structures, additional conserved motifs, and DNA-binding site specificity supported our classification scheme. Additionally, chromosomal distribution and collinearity analyses suggested that expansion of the PpbZIP transcription factor family was due to segment/chromosomal duplications. We also predicted the dimerization properties based on characteristic features of the leucine zipper and classified PpbZIP proteins into 23 subfamilies. Furthermore, qRT-PCR results indicated that PpbZIPs genes may be involved in regulating dormancy. The same gene of different species might participate in different regulating networks through interactions with specific partners. Our expression profiling results complemented the microarray data, suggesting that co-expression patterns of bZIP transcription factors during dormancy differed among deciduous fruit trees. Our findings further clarify the molecular characteristics of the PpbZIP transcription factor family, including potential gene functions during dormancy. This information may facilitate further research on the evolutionary history and biological functions of bZIP proteins in peach and other rosaceae plants.
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Ming-Yue
MY
Sun
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Xi-Ling
XL
Fu
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Qiu-Ping
QP
Tan
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Li
L
Liu
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Min
M
Chen
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Cui-Ying
CY
Zhu
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Ling
L
Li
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China.
Xiu-De
XD
Chen
College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China; Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Taian 271018, China. Electronic address: dsgao@sdau.edu.cn.
Dong-Sheng
DS
Gao
D000595:Amino Acid Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D032461:Chromosomes, Plant / Q000235:genetics; D017124:Conserved Sequence; D018744:DNA, Plant / Q000378:metabolism; D035264:Flowers / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007438:Introns / Q000235:genetics; D016350:Leucine Zippers / Q000235:genetics*; D005810:Multigene Family; D059372:Nucleotide Motifs / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055503:Protein Multimerization; D000068239:Prunus persica / Q000235:genetics*; D022821:RNA Splice Sites / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment
D050976:Basic-Leucine Zipper Transcription Factors; D018744:DNA, Plant; D010940:Plant Proteins; D022821:RNA Splice Sites
Microarray analysis; Phylogenetic analysis; Relative gene expression; bZIP transcription factor