Comparing the sugar profiles and primary structures of alkali-extracted water-soluble polysaccharides in cell wall between the yeast and mycelial phases from Tremella fuciformis.
To gain insights into dimorphism, cell wall polysaccharides from Tremella fuciformis strains were obtained from alkali-extracted water-soluble fractions PTF-M38 (from the mycelial form), PTF-Y3 and PTF-Y8 (from the yeast form) of T. fuciformis strains were used to gain some insights into dimorphism study. Their chemical properties and structural features were investigated using gel permeation chromatography, gas chromatography, UV and IR spectrophotometry and Congo red binding reactions. The results indicated that the backbones of PTF-M38, PTF-Y3 and PTF-Y8 were configured with α-linkages with average molecular weights of 1.24, 1.08, and 1.19 kDa, respectively. PTF-M38 was mainly composed of xylose, mannose, glucose, and galactose in a ratio of 1:1.47:0.48:0.34, while PTF-Y3 and PTF-Y8 were mainly composed of xylose, mannose and glucose in a ratio of 1:1.65:4.06 and 1:1.21:0.44, respectively. The sugar profiles of PTF-M38, PTF-Y3 and PTF-Y8 were also established for further comparison. These profiles showed that all three polysaccharides contained the same sugars but in different ratios, and the carbon sources (xylose, mannose, glucose, and galactose) affected the sugar ratios within the polysaccharides.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
Hanyu
H
Zhu
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
Yuan
Y
Yuan
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
Juan
J
Liu
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
Liesheng
L
Zheng
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China.
Liguo
L
Chen
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China. aiminma@mail.hzau.edu.cn.|Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, P. R. China. aiminma@mail.hzau.edu.cn.
Aimin
A
Ma
D001487:Basidiomycota / Q000737:chemistry* / Q000378:metabolism; D002236:Carbohydrate Conformation; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism; D014574:Uronic Acids / Q000032:analysis; D014867:Water / Q000737:chemistry
D002241:Carbohydrates; D062610:Fungal Polysaccharides; D014574:Uronic Acids; D014867:Water
Tremella fuciformis; cell wall polysaccharide; fungal dimorphism; structure analysis; sugar profile