In Vitro Callus Induction and Plant Regeneration from Stem Explants of Ceropegia noorjahaniae, a Critically Endangered Medicinal Herb.
An efficient protocol has been developed for in vitro regeneration of a large number of plantlets of Ceropegia noorjahaniae Ansari via indirect organogenesis from stem explants excised from in vitro-germinated seedlings. The callus was efficiently induced from the stem explants using Murashige and Skoog (MS) medium supplemented with auxins and their combinations. The highest number of shoots (16.0 ± 0.2) and shoot length (5.5 ± 0.1 cm) was achieved when the callus was subcultured to MS medium supplemented with 6-benzylaminopurine, BAP (2.0 mg/l) and indole-3-acetic acid, IAA (0.2 mg/l). The in vitro-developed shoots were rooted well in half-strength MS medium supplemented with 1.0 mg/l of indole-3-butyric acid (IBA) and 0.3 mg/l of α-naphthalene acetic acid (NAA). The plantlets were successfully hardened with 82 % survival rate. This is the first report on the regeneration of plants through indirect shoot organogenesis from stem derived calli of C. noorjahaniae.
Department of Botany, Laboratory of Plant Biotechnology and Biochemistry, Yashavantrao Chavan Institute of Science, Satara, 415001, India. jaychavansu@gmail.com.
Jaykumar J
JJ
Chavan
Department of Botany, Laboratory of Plant Biotechnology and Biochemistry, Yashavantrao Chavan Institute of Science, Satara, 415001, India.
Mahendra L
ML
Ahire
D000064:Acclimatization; D029062:Apocynaceae / Q000254:growth & development*; D001593:Benzyl Compounds / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods; D056727:Endangered Species; D007211:Indoles / Q000378:metabolism; D063246:Organogenesis, Plant; D010937:Plant Growth Regulators / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development*; D010946:Plants, Medicinal / Q000254:growth & development*; D011687:Purines / Q000378:metabolism
D001593:Benzyl Compounds; D003470:Culture Media; D007211:Indoles; D010937:Plant Growth Regulators; D011687:Purines; C014612:indolebutyric acid; C480551:benzylaminopurine
Acclimatization; C. noorjahaniae; Callus culture; Critically endangered; Medicinal herb; Organogenesis