Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants.
Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.
Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Belo Horizonte, Minas Gerais, Brazil.
Juliana P S
JP
Mol
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Belo Horizonte, Minas Gerais, Brazil.
Simone F
SF
Pires
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Toxinologia, Rio de Janeiro, Rio de Janeiro, Brazil.
Alexander D
AD
Chapeaurouge
Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Toxinologia, Rio de Janeiro, Rio de Janeiro, Brazil.
Jonas
J
Perales
Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Clínica e Cirurgia Veterinárias, Minas Gerais, Brasil.
Renato L
RL
Santos
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, Belo Horizonte, Minas Gerais, Brazil.
Hélida M
HM
Andrade
Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Belo Horizonte, Minas Gerais, Brazil.
Andrey P
AP
Lage
D000818:Animals; D002003:Brucella abortus / Q000502:physiology; D002007:Brucellosis, Bovine / Q000378:metabolism* / Q000382:microbiology; D002417:Cattle; D049033:Chorioallantoic Membrane / Q000378:metabolism* / Q000382:microbiology; D046508:Culture Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism / Q000382:microbiology
D020543:Proteome