A de novo self-assembling peptide hydrogel biosensor with covalently immobilised DNA-recognising motifs.
We report here the first experimental evidence of a self-assembling three-dimensional (3D) peptide hydrogel, with recognition motifs immobilized on the surface of fibres capable of sequence-specific oligonucleotide detection. These systems have the potential to be further developed into diagnostic and prognostic tools in human pathophysiology.
Manchester School of Pharmacy, the University of Manchester, Stopford Building, Oxford Road, Manchester, M13 9PT, UK. Elena.V.Bichenkova@manchester.ac.uk.
Patrick J S
PJ
King
School of Chemical Engineering and Analytical Science, Manchester Institute of Biotechnology, 131 Princess Street, Manchester, M1 7DN, UK. Aline.Miller@manchester.ac.uk.
Alberto
A
Saiani
Manchester School of Pharmacy, the University of Manchester, Stopford Building, Oxford Road, Manchester, M13 9PT, UK. Elena.V.Bichenkova@manchester.ac.uk.
Elena V
EV
Bichenkova
School of Chemical Engineering and Analytical Science, Manchester Institute of Biotechnology, 131 Princess Street, Manchester, M1 7DN, UK. Aline.Miller@manchester.ac.uk.
Aline F
AF
Miller
D015374:Biosensing Techniques; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis* / Q000737:chemistry; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013499:Surface Properties
D061925:Immobilized Nucleic Acids; D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate