Tooth color change caused by photosensitizers after photodynamic therapy: An in vitro study.
This study aimed to perform an in vitro evaluation of the effect of photosensitizers used in photodynamic therapy (PDT) on tooth color change when used in combination with conventional endodontic treatment. Forty extracted human mandibular premolars were accessed and underwent root canal therapy and PDT. Photosensitizers were used in accordance with the experimental groups: MB (n=10) - PDT with Methylene Blue at 0.01%; TB (n=10) - PDT with Toluidine Blue at 0.01%; MG (n=10) - PDT with Malachite Green at 0.01%, at the concentration of 0.1mg/mL; and PC (n=10) - positive control, PDT with Endo-PTC cream stained with Methylene Blue at 25%. The samples were irradiated with 660-nm diode laser by means of a 330-μm-diameter optical fiber cable at a power density of 40mW for 120s. After light curing, the photosensitizers were removed from the specimens with 10mL sodium hypochlorite at 1%. A reflectance spectrometer was used for evaluation of color prior to and 60days after the experimental procedure based on the CIE L*a*b* system. According to ANOVA test, there were statistically significant differences between the experimental groups (p=0.003). Tukey's test showed a significant difference between PC and TB (p=0.008), as well as between MG and TB (p=0.009). However, there was no statistically significant difference between PC, MG (p=0.957) and MB (p=0.103). It was concluded that the use of PDT as an adjuvant to root canal therapy, using different photosensitizers, led to color change in tooth structure.
Department of Dentistry, Federal University of Sergipe, Aracaju, SE, Brazil. Electronic address: larissa_odonto@yahoo.com.br.
Larissa Menezes
LM
Costa
State University of São Paulo, College of Dentistry, São José dos Campos, SP, Brazil. Electronic address: felipe_smatos@hotmail.com.
Felipe de Souza
Fde S
Matos
State University of São Paulo, College of Dentistry, São José dos Campos, SP, Brazil. Electronic address: aylamacyelle@hotmail.com.
Ayla Macyelle de Oliveira
AM
Correia
Department of Dentistry, Federal University of Sergipe, Aracaju, SE, Brazil. Electronic address: nayanecc@gmail.com.
Nayane Chagas
NC
Carvalho
Department of Dentistry, Federal University of Sergipe, Aracaju, SE, Brazil. Electronic address: fariaesilva.andre@gmail.com.
André Luís
AL
Faria-E-Silva
Department of Dentistry, Federal University of Sergipe, Aracaju, SE, Brazil. Electronic address: paranhos@ortodontista.com.br.
Luiz Renato
LR
Paranhos
Department of Dentistry, Federal University of Sergipe, Aracaju, SE, Brazil. Electronic address: endoribeiro@yahoo.com.br.
Maria Amália Gonzaga
MA
Ribeiro
D003116:Color; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007834:Lasers; D008751:Methylene Blue / Q000737:chemistry; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000737:chemistry; D013053:Spectrophotometry; D013997:Time Factors; D014048:Tolonium Chloride / Q000737:chemistry; D014070:Tooth / Q000737:chemistry*
D017319:Photosensitizing Agents; D014048:Tolonium Chloride; D012973:Sodium Hypochlorite; D008751:Methylene Blue
Coloring agents; Endodontics; Photochemotherapy; Photosensitizing agents; Spectrophotometry