Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers.
Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall materials from the cv. Kardal (wild-type, background) and two UGE transgenic lines (UGE 45-1 and UGE 51-16) were isolated and fractionated. The galactose (Gal) content (mg/100g tuber) from UGE 45-1 transgenic line was 38% higher than that of wild-type, and resulted in longer pectin side chains. The Gal content present in UGE 51-16 was 17% lower than that of wild-type, although most pectin populations maintained the same level of Gal. Both UGE transgenic lines showed unexpectedly a decrease in acetylation and an increase in methyl-esterification of pectin. Both UGE transgenic lines showed similar proportions of homogalacturonan and rhamnogalacturonan I within pectin backbone as the wild-type, except for the calcium-bound pectin fraction exhibiting relatively less rhamnogalacturonan I. Next to pectin modification, xyloglucan populations from both transgenic lines were altered resulting in different XSGG and XXGG proportion in comparison to wild-type.
Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Electronic address: jiehonghuang0216@gmail.com.
Jie-Hong
JH
Huang
Wageningen UR Plant Breeding, Wageningen University and Research Centre, Wageningen, The Netherlands. Electronic address: anne.kortstee@outlook.com.
Anne
A
Kortstee
Wageningen UR Plant Breeding, Wageningen University and Research Centre, Wageningen, The Netherlands. Electronic address: dianka.dees@wur.nl.
Dianka C T
DC
Dees
Wageningen UR Plant Breeding, Wageningen University and Research Centre, Wageningen, The Netherlands. Electronic address: luisa.trindade@wur.nl.
Luisa M
LM
Trindade
Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Electronic address: henk.schols@wur.nl.
Henk A
HA
Schols
Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands. Electronic address: harry.gruppen@wur.nl.
Harry
H
Gruppen
D000818:Animals; D002473:Cell Wall / Q000737:chemistry* / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D035281:Plant Tubers / Q000737:chemistry* / Q000166:cytology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics; D011134:Polysaccharides / Q000737:chemistry* / Q000235:genetics*; D011198:Solanum tuberosum / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D014534:UDPglucose 4-Epimerase / Q000235:genetics / Q000378:metabolism
D011134:Polysaccharides; D014534:UDPglucose 4-Epimerase
Cell wall polysaccharides; Nucleotide sugar interconversion; Plant biosynthesis; Solanum tuberosum; Transgenic potato; UDP-sugars