The biofilm matrix of Campylobacter jejuni determined by fluorescence lectin-binding analysis.
Campylobacter jejuni is responsible for the most common bacterial foodborne gastroenteritis. Despite its fastidious growth, it can survive harsh conditions through biofilm formation. In this work, fluorescence lectin-binding analysis was used to determine the glycoconjugates present in the biofilm matrix of two well-described strains. Screening of 72 lectins revealed strain-specific patterns with six lectins interacting with the biofilm matrix of both strains. The most common sugar moiety contained galactose and N-acetylgalactosamine. Several lectins interacted with N-acetylglucosamine and sialic acid, probably originated from the capsular polysaccharides, lipooligosaccharides and N-glycans of C. jejuni. In addition, glycoconjugates containing mannose and fucose were detected within the biofilm, which have not previously been found in the C. jejuni envelope. Detection of thioflavin T and curcumin highlighted the presence of amyloids in the cell envelope without association with specific cell appendages. The lectins ECA, GS-I, HMA and LEA constitute a reliable cocktail to detect the biofilm matrix of C. jejuni.
a Department of Microbiology & Food Chain , SECALIM UMR1014, INRA , Nantes , France.|b LUNAM Université, Oniris, Université de Nantes , Nantes , France.|c Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology , University of Chemistry and Technology , Prague , Czech Republic.
Hana
H
Turonova
d Department of River Ecology , Helmholtz Centre for Environmental Research - UFZ , Magdeburg , Germany.
Thomas R
TR
Neu
c Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology , University of Chemistry and Technology , Prague , Czech Republic.
Pavel
P
Ulbrich
c Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology , University of Chemistry and Technology , Prague , Czech Republic.
Jarmila
J
Pazlarova
a Department of Microbiology & Food Chain , SECALIM UMR1014, INRA , Nantes , France.|b LUNAM Université, Oniris, Université de Nantes , Nantes , France.
Odile
O
Tresse
D018441:Biofilms; D016123:Campylobacter jejuni / Q000502:physiology*; D005453:Fluorescence; D006001:Glycoconjugates / Q000032:analysis; D037102:Lectins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis
D006001:Glycoconjugates; D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C023023:lipid-linked oligosaccharides
CLSM; Campylobacter jejuni; FLBA; amyloids; biofilm matrix