Associations of fractalkine receptor (CX3CR1) and CCR5 gene variants with hypertension, diabetes and atherosclerosis in chronic renal failure patients undergoing hemodialysis.
OBJECTIVE
We aimed to investigate the associations of fractalkine receptor (CX3CR1) V249I, T280M and CCR5-59029 A/G gene polymorphisms in chronic renal failure (CRF) subjects undergoing hemodialysis and to evaluate possible associations of these polymorphisms with hypertension (HT), diabetes mellitus (DM) and atherosclerosis (AS).


METHODS
A total of 225 CRF subjects undergoing hemodialysis and 201 healthy controls were enrolled in the study. CRF subjects were divided into three major subgroups according to comorbidities including HT (n = 127), DM (n = 65) and AS (n = 33). Genotyping was done using polymerase chain reaction-restriction fragment length polymorphism method.


RESULTS
The II genotype and I allele frequencies of CX3CR1 V249I polymorphism were found significantly more frequent in CRF subjects, CRF subjects with DM and CRF subjects with AS compared with controls (p < 0.05 for all comparisons). G allele frequency of CCR5 polymorphism was found significantly more prevalent in CRF subjects with DM than that of controls. Further, GG genotype and G allele frequencies of CCR5 polymorphism were significantly more prevalent in CRF subjects with AS compared with controls (p < 0.05). We also explored these polymorphisms among CRF subjects with and without following comorbidities: HT, DM, AS. We found significant association between CRF subjects with HT and without HT in terms of genotype and allele frequencies of V249I polymorphism (p < 0.05). CX3CR1 T280M polymorphism was not found significantly different in none of the comparisons.


CONCLUSIONS
These data demonstrate possible associations between CX3CR1 V249I and CCR5-59029 A/G polymorphisms and/or HT, DM and AS in CRF subjects.
Department of Nutrition and Dietetics, School of Health Sciences, Cumhuriyet University, 58140, Sivas, Turkey. binnur.koksal@hotmail.com.|Advanced Technology Research Center, Cumhuriyet University, Sivas, Turkey. binnur.koksal@hotmail.com.
Binnur
B
Bagci
Department of Medical Genetics, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Gokhan
G
Bagci
Division of Nephrology, Department of Internal Medicine, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Can
C
Huzmeli
Department of Medical Genetics, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Ilhan
I
Sezgin
Department of Medical Genetics, School of Medicine, Canakkale On Sekiz Mart University, Canakkale, Turkey.
Ozturk
O
Ozdemir
D000368:Aged; D050197:Atherosclerosis / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics; D000073883:CX3C Chemokine Receptor 1; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015897:Comorbidity; D003920:Diabetes Mellitus / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics; D007676:Kidney Failure, Chronic / Q000453:epidemiology / Q000235:genetics* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D019713:Receptors, CCR5 / Q000235:genetics*; D019707:Receptors, Chemokine / Q000235:genetics*; D012016:Reference Values; D006435:Renal Dialysis / Q000379:methods* / Q000401:mortality; D016019:Survival Analysis; D016896:Treatment Outcome
C584933:CCR5 protein, human; D000073883:CX3C Chemokine Receptor 1; C095235:CX3CR1 protein, human; D019713:Receptors, CCR5; D019707:Receptors, Chemokine
Atherosclerosis; CCR5; CX3CR1; Chronic renal failure; Diabetes mellitus; Hypertension; Polymorphism