Coenzyme Q10 analytical determination in biological matrices and pharmaceuticals.
In recent years, the analytical determination of coenzyme Q10 (CoQ10) has gained importance in clinical diagnosis and in pharmaceutical quality control. CoQ10 is an important cofactor in the mitochondrial respiratory chain and a potent endogenous antioxidant. CoQ10 deficiency is often associated with numerous diseases and patients with these conditions may benefit from administration of supplements of CoQ10. In this regard, it has been observed that the best benefits are obtained when CoQ10 deficiency is diagnosed and treated early. Therefore, it is of great value to develop analytical methods for the detection and quantification of CoQ10 in this type of disease. The methods above mentioned should be simple enough to be used in routine clinical laboratories as well as in quality control of pharmaceutical formulations containing CoQ10. Here, we discuss the advantages and disadvantages of different methods of CoQ10 analysis.
Consejo Nacional de Investigaciones Científicas y Tecnologicas, CONICET, Argentina and Departamento de Tecnología Farmaceutica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Silvia
S
Lucangioli
Departamento de Tecnología Farmaceutica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Manuela
M
Martinefski
Consejo Nacional de Investigaciones Científicas y Tecnologicas, CONICET, Argentina and Departamento de Tecnología Farmaceutica, Universidad de Buenos Aires, Buenos Aires, Argentina, vtripodi@ffyb.uba.ar.
Valeria
V
Tripodi
D001259:Ataxia / Q000175:diagnosis / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D006801:Humans; D028361:Mitochondrial Diseases / Q000175:diagnosis / Q000201:enzymology; D018908:Muscle Weakness / Q000175:diagnosis / Q000201:enzymology; D004364:Pharmaceutical Preparations / Q000737:chemistry; D013053:Spectrophotometry; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000172:deficiency / Q000302:isolation & purification
D004364:Pharmaceutical Preparations; D014451:Ubiquinone; C024989:coenzyme Q10