Highly Sensitive Detection of Melamine Using a One-Step Sample Treatment Combined with a Portable Ag Nanostructure Array SERS Sensor.
There is an urgent need for rapid and reliable methods able to detect melamine in animal feed. In this study, a quick, simple, and sensitive method for the determination of melamine content in animal feed was developed using surface-enhanced Raman spectroscopy on fabricated Ag nanorod (AgNR) array substrates with a one-step sample extraction procedure. The AgNR array substrates washed by HNO3 solvent (10-7 M) and methanol and showed the good stability within 6 months. The Raman shift at △ν = 682 cm-1 was used as the characteristic melamine peak in the calculations. Sufficient linearity was obtained in the 2-200 μg·g-1 range (R2 = 0.926). The limits of detection and quantification were 0.9 and 2 μg·g-1, respectively. The recovery rates were 89.7-93.3%, with coefficients of variation below 2.02%. The method showed good accuracy compared with the tradition GC-MS analysis. This new protocol only need 2 min to fininsh the detection which could be developed for rapid onsite screening of melamine contamination in quality control and market surveillance applications.
Institute of Quality Standards and Testing Technologies for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, China.
Jie
J
Cheng
Institute of Quality Standards and Testing Technologies for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, China.
Xiao-Ou
XO
Su
School of Physics and Electronic Engineering, Jiangsu Normal University, Jiangsu, China.
Yue
Y
Yao
School of Physics and Electronic Engineering, Jiangsu Normal University, Jiangsu, China.
Caiqin
C
Han
Institute of Quality Standards and Testing Technologies for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, China.
Shi
S
Wang
Department of Physics and Astronomy, University of Georgia, Athens, United States of America.
Yiping
Y
Zhao
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002623:Chemistry Techniques, Analytical; D005506:Food Contamination / Q000032:analysis; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D000432:Methanol; D043942:Nanotubes / Q000737:chemistry; D012117:Resins, Synthetic / Q000032:analysis*; D012997:Solvents; D013059:Spectrum Analysis, Raman / Q000379:methods; D014227:Triazines / Q000032:analysis*
D012117:Resins, Synthetic; D012997:Solvents; D014227:Triazines; D006046:Gold; C011907:melamine; D000432:Methanol