Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d'Ivoire versus Gabon.
Introduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d'Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp1 and msp2 genes. Results. The K1 allelic family was widespread in Côte d'Ivoire (64.6%) and in Gabon (56.6%). For msp2, the 3D7 alleles were more prevalent (>70% in both countries) compared to FC27 alleles. In Côte d'Ivoire, the frequencies of multiple infections with msp1 (45.1%) and msp2 (40.3%) were higher than those found for isolates from Gabon, that is, 30.2% with msp1 and 31.4% with msp2. The overall complexity of infection was 1.66 (SD = 0.79) in Côte d'Ivoire and 1.58 (SD = 0.83) in Gabon. It decreased with age in Côte d'Ivoire in contrast to Gabon. Conclusion. Differences observed in some allelic families and in complexity profile may suggest an impact of epidemiological facies as well as immunological response on genetic variability of P. falciparum.
Malaria Research and Control Centre, National Institute of Public Health, BPV 47, Abidjan, Côte d'Ivoire; Faculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d'Ivoire.
William
W
Yavo
Malaria Research and Control Centre, National Institute of Public Health, BPV 47, Abidjan, Côte d'Ivoire; Faculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d'Ivoire.
Abibatou
A
Konaté
Faculty of Medicine, Department of Parasitology and Mycology, University des Sciences de la Santé, BP 4009, Libreville, Gabon.
Denise Patricia
DP
Mawili-Mboumba
Faculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d'Ivoire; Parasitology and Mycology Laboratory of Diagnosis and Research Centre on AIDS and Other Infectious Diseases, 01 BPV 03, Abidjan, Côte d'Ivoire.
Fulgence Kondo
FK
Kassi
Faculty of Medicine, Department of Parasitology and Mycology, University des Sciences de la Santé, BP 4009, Libreville, Gabon.
Marie L
ML
Tshibola Mbuyi
Faculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d'Ivoire.
Etienne Kpongbo
EK
Angora
Faculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d'Ivoire; Parasitology and Mycology Laboratory of Diagnosis and Research Centre on AIDS and Other Infectious Diseases, 01 BPV 03, Abidjan, Côte d'Ivoire.
Eby I Hervé
EI
Menan
Faculty of Medicine, Department of Parasitology and Mycology, University des Sciences de la Santé, BP 4009, Libreville, Gabon.
Marielle K
MK
Bouyou-Akotet