The development of cryo-EM into a mainstream  structural biology technique.
Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades as a technique capable of studying challenging systems that otherwise defy structural characterization. Recent technical advances have resulted in a 'quantum leap' in applicability, throughput and achievable resolution that has gained this technique worldwide attention. Here I discuss some of the major historical landmarks in the development of the cryo-EM field, ultimately leading to its present success.
Eva
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Nogales
D020285:Cryoelectron Microscopy / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry*
D046911:Macromolecular Substances