Niabellapedocola sp. nov., isolated from soil.
An aerobic, Gram-stain-negative, oxidase-positive and catalase-negative, non-motile, non-spore-forming, rod-shaped, orange-pigmented bacterium designated strain R384Twas isolated from soil. Flexirubin-type pigments were present. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain R384Tformed a lineage within the family Chitinophagaceae of the phylum Bacteroidetes that was distinct from various species of the genus Niabella, including 'Niabella thaonhiensis' NHI-24 (98.47 % sequence similarity), Niabellaaurantiaca DSM 17617T(96.89 %), Niabelladrilacis E90T(96.63 %), Niabellahirudinis E96T(96.61 %), Niabellatibetensis 15-4T(95.53 %), Niabellasoli DSM 19437T(94.81 %), Niabellaginsengisoli GR10-1T(94.19 %) and Niabellayanshanensis CCBAU 05354T(93.67 %). The major isoprenoid quinone was menaquinone-7 (MK-7) and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH. The DNA G+C content of strain R384Twas 44.7 mol %. DNA-DNA hybridization values between strain R384Tand other members of the genus Niabella ranged from 26 to 55 %. On the basis of phenotypic, genotypic and phylogenetic analysis, strain R384Trepresents a novel species of the genus Niabella, for which the name Niabellapedocola sp. nov. is proposed. The type strain is R384T(=KEMB 9005-329T=KACC 18454T=JCM 31011T).
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 443-760, South Korea.
Ram Hari
RH
Dahal
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 443-760, South Korea.
Jaisoo
J
Kim
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7
Bacteroidetes; Chitinophagaceae; Niabellapedocola sp. nov.,