Extracting Subcellular Fibrillar Alignment with Error Estimation: Application to Microtubules.
The order and orientation of cortical microtubule (CMT) arrays and their dynamics play an essential role in plant morphogenesis. To extract detailed CMT alignment structures in an objective, local, and accurate way, we propose an error-based extraction method that applies to general fluorescence intensity data on three-dimensional cell surfaces. Building on previous techniques to quantify alignments, our method can determine the statistical error for specific local regions, or the minimal scales of local regions for a desired accuracy goal. After validating our method with synthetic images with known alignments, we demonstrate the ability of our method to quantify subcellular CMT alignments on images with microtubules marked with green fluorescent protein in various cell types. Our method could also be applied to detect alignment structures in other fibrillar elements, such as actin filaments, cellulose, and collagen.
Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0020 Japan.
Satoru
S
Tsugawa
Plant Reproduction and Development Lab., INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, Lyon, France.
Nathan
N
Hervieux
Plant Reproduction and Development Lab., INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, Lyon, France.
Oliver
O
Hamant
Plant Reproduction and Development Lab., INRA, CNRS, ENS Lyon, UCB Lyon 1, Université de Lyon, Lyon, France.
Arezki
A
Boudaoud
Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Richard S
RS
Smith
Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0020 Japan. Electronic address: cbli@es.hokudai.ac.jp.
Chun-Biu
CB
Li
Research Institute for Electronic Science, Hokkaido University, Sapporo 001-0020 Japan. Electronic address: tamiki@es.hokudai.ac.jp.
Tamiki
T
Komatsuzaki
D017360:Arabidopsis / Q000166:cytology; D008870:Microtubules / Q000378:metabolism*; D057054:Molecular Imaging