Single-molecule detection at high concentrations with optical aperture nanoantennas.
Single-molecule detection has become an indispensable technology in life science, and medical research. In order to get meaningful information on many biological processes, single-molecule analysis is required in micro-molar concentrations. At such high concentrations, it is very challenging to isolate a single molecule with conventional diffraction-limited optics. Recently, optical aperture nanoantennas (OANs) have emerged as a powerful tool to enhance the single-molecule detection under a physiological environment. The OANs, which consist of nano-scale apertures on a metallic film, have the following unique properties: (1) nanoscale light confinement; (2) enhanced fluorescence emission; (3) tunable radiation pattern; (4) reduced background noise; and (5) massive parallel detection. This review presents the fundamentals, recent developments and future perspectives in this emerging field.
Electro-Optics Program, University of Dayton, 300 College Park, Dayton, Ohio 45469-2314, USA. czhao1@udayton.edu.
Md Shah
MS
Alam
Farzia
F
Karim
Chenglong
C
Zhao