TMEM45B promotes proliferation, invasion and migration and inhibits apoptosis in pancreatic cancer cells.
In the present study, we focused on the expression and biological functions of TMEM45B in pancreatic cancer tissues and cell lines. Real-time PCR and Western blotting were used to examine the expression levels of TMEM45B in pancreatic cancer tissues and cell lines. The functions of TMEM45B were evaluated using CCK-8, flow cytometry and transwell analysis. Our results showed that TMEM45B exhibited high expression in pancreatic cancer tissues and cell lines compared with the normal pancreatic tissues and cells. Using gene set enrichment analysis (GSEA), we found that TMEM45B may regulate multiple genes involved in the cell cycle and metastasis pathways. Downregulation of TMEM45B by RNA interference significantly reduced proliferation, invasion and migration of SW1990 and PANC-1 cells, accompanied by the induction of cell cycle arrest and apoptosis, whereas overexpression of TMEM45B promoted proliferation, invasion and migration of CFPAC-1 cells as well as apoptosis inhibition. Taken together, our study provides evidence that TMEM45B is an oncogene involved in the tumorigenesis of pancreatic cancer and may represent a new molecular target for pancreatic cancer treatment.
Department of General Surgery, Ruijin Hospital North Affiliated to Shanghai Jiaotong University School of Medicine, 999 Xiwang Road, Jiading District, Shanghai 201801, China.
Liang-Chao
LC
Zhao
Department of General Surgery, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 197 Ruijin 2 Road, Huangpu District, Shanghai 200025, China. zhuzhenggangsh@163.com chhpeng@188.com.
Bai-Yong
BY
Shen
Department of General Surgery, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 197 Ruijin 2 Road, Huangpu District, Shanghai 200025, China. zhuzhenggangsh@163.com chhpeng@188.com.
Xia-Xing
XX
Deng
Department of General Surgery, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 197 Ruijin 2 Road, Huangpu District, Shanghai 200025, China. zhuzhenggangsh@163.com chhpeng@188.com.
Hao
H
Chen
Department of General Surgery, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 197 Ruijin 2 Road, Huangpu District, Shanghai 200025, China. zhuzhenggangsh@163.com chhpeng@188.com.
Zheng-Gang
ZG
Zhu
Department of General Surgery, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 197 Ruijin 2 Road, Huangpu District, Shanghai 200025, China. zhuzhenggangsh@163.com chhpeng@188.com.
Cheng-Hong
CH
Peng
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D047368:Tumor Burden
D008565:Membrane Proteins; C000614275:TMEM45B protein, human