Assessment of Fatty Acid Allocation in Plasma and Tissues in Piglets, Using Feed Supplemented with Byproducts from Processed Olive Mill Wastewater.
OBJECTIVE
A previous study revealed the improvement of redox status in blood and tissues of young piglets (ablactation period), that consumed feed containing polyphenolic additives from byproducts of processed olive mill wastewater (OMWW). The polyphenolic additives strengthened the antioxidant defense of the piglets. Herein we analyzed the fatty acid (FA) composition of these animals in various tissues.


METHODS
The steps followed during the analysis were: Preparation and isolation of byproducts containing polyphenolic compounds from OMWW processing, silage and piglet feed preparation, blood and tissue collection, fatty acid methyl esters synthesis and GC/MS analysis.


RESULTS
The young piglets, that consumed feed containing polyphenolic additives from byproducts of processed OMWW, were found to have a decreased ω6/ω3 ratio, compared to samples of the control group. For example, in the quadriceps tissue the control group has a ω6/ω3 ratio of 10.1, while in the polyphenolic group this ratio was decreased to 2.93. Regarding the ratio of UFA/SFA, no significant differences were observed. Finally, the polyphenolic group exhibited almost in all tissues lower values of the ratio of PUFA/MUFA than the control group.
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece Department of Biosystem Engineering, Technical Education Institute of Thessaly, Larissa, Greece.
Konstantinos
K
Gerasopoulos
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Dimitrios
D
Stagos
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Alexandros
A
Krouezas
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Christina
C
Karaveli
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Christina
C
Barda
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Helen
H
Gkika
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Dimitrios
D
Mitsiou
Department of Biosystem Engineering, Technical Education Institute of Thessaly, Larissa, Greece.
Konstantinos
K
Petrotos
Department of Biosystem Engineering, Technical Education Institute of Thessaly, Larissa, Greece.
Panagiotis
P
Goulas
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece dkouret@uth.gr.
Demetrios
D
Kouretas
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis; D019587:Dietary Supplements; D005227:Fatty Acids / Q000032:analysis* / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000032:analysis / Q000097:blood; D043371:Fatty Acids, Omega-6 / Q000032:analysis / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D007220:Industrial Waste; D031658:Olea; D010084:Oxidation-Reduction / Q000187:drug effects; D059808:Polyphenols / Q000008:administration & dosage* / Q000302:isolation & purification; D013552:Swine; D062065:Wastewater / Q000737:chemistry*
D000975:Antioxidants; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D007220:Industrial Waste; D059808:Polyphenols; D062065:Wastewater
Olive mill wastewater; antioxidant feed; fatty acid; piglet; ω6/ω3 ratio