An untargeted approach for the analysis of the urine peptidome of women with preeclampsia.
Preeclampsia (PE) is a pregnancy complication characterized by high blood pressure and proteinuria. The disorder usually occurs after the 20th week of pregnancy and gets worse over time. PE increases the risk of poor outcomes for both the mother and the baby. In the study we applied LC-MS/MS method for the analysis of the urine peptidome of women with PE. Samples were prepared using size-exclusion chromatography method which gives more than twice peptides identities if compared with solid phase extraction. Thirty urine samples from women with mild and severe preeclampsia and the control group were analyzed. In total 1786 peptides were identified using complementary search engines (Mascot, MaxQuant and PEAKS). A high level of agreement in peptide identification was observed with previously published data. Label-free data comparison resulted in 35 peptides which reliably distinguished a particular PE group (severe or mild) from controls. Our results revealed unique identifications (correlate to alpha-1-antitrypsin, collagen alpha-1(I) chain, collagen alpha-1 (III) chain, and uromodulin, for instance) that can potentially serve as early indicators of PE.
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia.; Moscow Institute of Physics and Technology, Institutskiy per., 9, Dolgoprudny, Moscow Region 141700, Russia.
A S
AS
Kononikhin
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia.; Moscow Institute of Physics and Technology, Institutskiy per., 9, Dolgoprudny, Moscow Region 141700, Russia.
N L
NL
Starodubtseva
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
A E
AE
Bugrova
Moscow Institute of Physics and Technology, Institutskiy per., 9, Dolgoprudny, Moscow Region 141700, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia.
V A
VA
Shirokova
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
V V
VV
Chagovets
Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia.
M I
MI
Indeykina
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; Moscow Institute of Physics and Technology, Institutskiy per., 9, Dolgoprudny, Moscow Region 141700, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia.; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
I A
IA
Popov
Moscow Institute of Physics and Technology, Institutskiy per., 9, Dolgoprudny, Moscow Region 141700, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia.
Y I
YI
Kostyukevich
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
O V
OV
Vavina
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
K T
KT
Muminova
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
Z S
ZS
Khodzhaeva
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
N E
NE
Kan
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
V E
VE
Frankevich
Moscow Institute of Physics and Technology, Institutskiy per., 9, Dolgoprudny, Moscow Region 141700, Russia; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia.. Electronic address: ennikolaev@rambler.ru.
E N
EN
Nikolaev
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
G T
GT
Sukhikh
D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000652:urine; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D010455:Peptides / Q000652:urine; D011225:Pre-Eclampsia / Q000652:urine*; D011247:Pregnancy; D020543:Proteome / Q000032:analysis*; D052616:Solid Phase Extraction; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D010455:Peptides; D020543:Proteome
Endogenous peptides; LC-MSMS; Preeclamsia; Size-exclusion chromatography; Solid-phase extraction; Urine peptidome