Biochemical Basis of CO2-Related Internal Browning Disorders in Pears (Pyrus communis L. cv. Rocha) during Long-Term Storage.
This study aimed at understanding the biochemical basis of internal browning disorders (IBDs) in 'Rocha' pear. For this purpose, the effects of storage under normal controlled atmosphere (CA) (3 kPa of O2 + 0.5 kPa of CO2) and IBD-inducing CA (1 kPa of O2 + 10 kPa of CO2) on the antioxidant and fermentative metabolisms and polyphenol oxidase (PPO) activity and phenolics concentration were studied. The higher IBD incidence in high CO2-stored fruits was positively correlated with fermentative metabolites and negatively with ascorbate and H2O2 concentrations, and it was linked to PPO activation. These results indicate that both the antioxidant and fermentative metabolisms are involved in the occurrence of IBD in 'Rocha' pear. From the integration of the biochemical and enzymatic data, a schematic model illustrating the effects of high CO2 and low O2 in 'Rocha' pears during long-term storage was constructed.
CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia Universidade Católica Portuguesa , Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Teresa
T
Deuchande
IRTA , Postharvest Department, Parc Cientific i Tecnològic Agroalimentari, Parc de Gardeny, Edifici Fruitcentre, 25003 Lleida, Spain.
Christian
C
Larrigaudière
IRTA , Postharvest Department, Parc Cientific i Tecnològic Agroalimentari, Parc de Gardeny, Edifici Fruitcentre, 25003 Lleida, Spain.
Jordi
J
Giné-Bordonaba
CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia Universidade Católica Portuguesa , Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.|Departamento de Geociências Ambiente e Ordenamento do Território, Faculdade de Ciências, Universidade do Porto , Rua do Campo Alegre 697, 4169-007 Porto, Portugal.|GreenUP/CITAB-UP & DGAOT, Faculty of Sciences, University of Porto , Campus Agrário de Vairão, Rua Padre Armando Quintas 7, 4485-661 Vairão, Portugal.|Department of Plant Sciences, Horticulture and Product Physiology Group, Wageningen University , Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
Susana M P
SM
Carvalho
CBQF - Centro de Biotecnologia e Quı́mica Fina - Laboratório Associado, Escola Superior de Biotecnologia Universidade Católica Portuguesa , Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Marta W
MW
Vasconcelos
D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001272:Atmosphere / Q000032:analysis; D002245:Carbon Dioxide / Q000032:analysis*; D004156:Catechol Oxidase / Q000032:analysis / Q000378:metabolism; D003116:Color; D061353:Food Storage / Q000295:instrumentation / Q000379:methods*; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D031989:Pyrus / Q000737:chemistry* / Q000378:metabolism
D000975:Antioxidants; D010636:Phenols; D010940:Plant Proteins; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D004156:Catechol Oxidase; D010100:Oxygen
Pyrus communis.; antioxidant system; controlled-atmosphere storage; fermentative metabolism; hydrogen peroxide; polyphenol oxidase