[In vitro dissolution rate of Liuwei Wuling tablet based on biological potency and integrated dissolution].
To explore the feasibility of chemical and biological method in evaluation of the in vitro dissolution rate of Liuwei Wuling tablet (LWT), this experiment investigated the inhibitory effect of LWT dissolving solutions on LX-2 hepatic stellate cells in 0.1% SDS dissolution medium in different dissolving periods. From these results, the cumulative dissolution rate of LWT was obtained based on the cell inhibitory rate. The dissolution rates of deoxyschizandrin, phillyrin, and Specnuezhenide were determined by HPLC method. A novel approach of self-defined weighting coefficient had been created to establish the integrated dissolution rate model. Then f2 similar factor method was used to evaluate the relevance of these two methods. The results showed that f2 values for deoxyschizandrin, phillyrin, Specnuezhenide, and the integrated dissolution were 61, 43, 61 and 75 respectively, indicating that the dissolution of multi-component integration could fully reflect the biological potency of the whole recipe. The dissolution evaluation method for multicomponent integration based on biological activity is expected to be one of the effective means for in vitro dissolution test of LWT.
Juan
J
Zheng
Ling
L
Cheng
Cheng-ying
CY
Shen
Juan-juan
JJ
Li
Ling
L
Qiu
Gang
G
Shen
Jin
J
Han
Hai-long
HL
Yuan
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007700:Kinetics; D011786:Quality Control; D012995:Solubility; D013607:Tablets / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D013607:Tablets; C533472:wuling