Binding kinetics and multi-bond: Finding correlations by synthesizing interactions between ligand-coated bionanoparticles and receptor surfaces.
The number of bonds formed between one single bionanoparticle and many surface receptors is an important subject to be studied but is seldom quantitatively investigated. A new evaluation of the correlation between binding kinetics and number of bonds is presented by varying ligand density and receptor density. An experimental system was developed using measurements with surface plasmon resonance spectroscopy. A corresponding multi-site adsorption model elucidated the correlation. The results show that with the increase of the receptor density, the adsorption rate first decreased when the number of bonds was below a maximum value and then increased when the number of bonds stayed at this maximum value. The investigation on ligand density variation suggests that the coating density on top of the bionanoparticle surface may have a particular value below which more ligand will accelerate the adsorption rate. The ratio of ligand amount bound by the receptors to the total ligand amount associated with a single bionanoparticle will remain constant even if one attaches more ligands to a bionanoparticle. We envision that the bionanoparticle desorption will not depend on density changes from either ligand or receptor when the number of bonds reaches a specific efficient value.
Max Planck Institute for Dynamics of Complex Technical Systems, D-39106 Magdeburg, Germany. Electronic address: wangw@mpi-magdeburg.mpg.de.
Wenjing
W
Wang
Chair for Process Systems Engineering, Otto-von-Guericke University Magdeburg, D-39106 Magdeburg, Germany.
Andreas
A
Voigt
Max Planck Institute for Dynamics of Complex Technical Systems, D-39106 Magdeburg, Germany; Chair for Bioprocess Engineering, Otto-von-Guericke University Magdeburg, D-39106 Magdeburg, Germany.
Michael W
MW
Wolff
Max Planck Institute for Dynamics of Complex Technical Systems, D-39106 Magdeburg, Germany; Chair for Bioprocess Engineering, Otto-von-Guericke University Magdeburg, D-39106 Magdeburg, Germany.
Udo
U
Reichl
Max Planck Institute for Dynamics of Complex Technical Systems, D-39106 Magdeburg, Germany; Chair for Process Systems Engineering, Otto-von-Guericke University Magdeburg, D-39106 Magdeburg, Germany.
Kai
K
Sundmacher
D000327:Adsorption; D001665:Binding Sites; D006801:Humans; D007700:Kinetics; D008024:Ligands; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D008024:Ligands; D011108:Polymers; D011956:Receptors, Cell Surface
Affinity; Antibody; Avidity; Binding kinetics; Conjugation