The interactome of Streptococcus pneumoniae and its bacteriophages show highly specific patterns of interactions among bacteria and their phages.
Although an abundance of bacteriophages exists, little is known about interactions between their proteins and those of their bacterial hosts. Here, we experimentally determined the phage-host interactomes of the phages Dp-1 and Cp-1 and their underlying protein interaction network in the host Streptococcus pneumoniae. We compared our results to the interaction patterns of E. coli phages lambda and T7. Dp-1 and Cp-1 target highly connected host proteins, occupy central network positions, and reach many protein clusters through the interactions of their targets. In turn, lambda and T7 targets cluster to conserved and essential proteins in E. coli, while such patterns were largely absent in S. pneumoniae. Furthermore, targets in E. coli were mutually strongly intertwined, while targets of Dp-1 and Cp-1 were strongly connected through essential and orthologous proteins in their immediate network vicinity. In both phage-host systems, the impact of phages on their protein targets appears to extend from their network neighbors, since proteins that interact with phage targets were located in central network positions, have a strong topologically disruptive effect and touch complexes with high functional heterogeneity. Such observations suggest that the phages, biological impact is accomplished through a surprisingly limited topological reach of their targets.
Dept. of Computer Science, University of Miami, Coral Gables, FL 33146, USA.
Rachelle
R
Mariano
Dept. of Computer Science, University of Miami, Coral Gables, FL 33146, USA.|Center for Computational Science, University of Miami, Coral Gables, FL 33146, USA.
Stefan
S
Wuchty
Max von Pettenkofer-Institute, Department of Virology, Ludwig-Maximilians-University, Munich, Germany.|Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Fisiología, Facultad de Medicina, UNT. San Miguel de Tucumán, Argentina.
Maria G
MG
Vizoso-Pinto
Genomics and Proteomics Core Facilities, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
Roman
R
Häuser
Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, VA 23284, USA.
Peter
P
Uetz
D001426:Bacterial Proteins / Q000378:metabolism; D017123:Bacteriophage T7 / Q000502:physiology; D010582:Bacteriophage lambda / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology / Q000821:virology; D006790:Host-Parasite Interactions; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D017108:Streptococcus Phages / Q000502:physiology*; D013296:Streptococcus pneumoniae / Q000502:physiology* / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D014764:Viral Proteins