Single-cell epigenomics: powerful new methods for understanding gene regulation and cell identity.
Emerging single-cell epigenomic methods are being developed with the exciting potential to transform our knowledge of gene regulation. Here we review available techniques and future possibilities, arguing that the full potential of single-cell epigenetic studies will be realized through parallel profiling of genomic, transcriptional, and epigenetic information.
Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK.
Stephen J
SJ
Clark
Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK. heather.lee@babraham.ac.uk.|Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. heather.lee@babraham.ac.uk.
Heather J
HJ
Lee
Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK.|Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058, Basel, Switzerland.
Sébastien A
SA
Smallwood
Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK.|Centre for Trophoblast Research, University of Cambridge, Cambridge, CB2 3EG, UK.
Gavin
G
Kelsey
Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK.|Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.|Centre for Trophoblast Research, University of Cambridge, Cambridge, CB2 3EG, UK.|Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, CB2 3EG, UK.
Wolf
W
Reik
D000818:Animals; D019175:DNA Methylation; D057890:Epigenomics / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D059010:Single-Cell Analysis / Q000379:methods*