Morphology of Nyctotheroides hubeiensis Li et al. 1998 from Frog Hosts with Molecular Phylogenetic Study of Clevelandellid Ciliates (Armophorea, Clevelandellida).
The morphology of Nyctotheroides hubeiensis (Acta Hydrobiol. Sin. 1998, 22(suppl.):187), collected from the rectum of Phelophylax nigromaculatus, is presented in this paper based on detailed morphological information and molecular data. Our phylogenetic analysis showed that N. hubeiensis fell into the Nyctotheroides clade, which was strongly supported as monophyletic and clustered as basal to the genera Nyctotherus and Clevelandella. Also, the monophyly of the Order Clevelandellida and the affinity of parasitic nyctotherids and free-living metopids were indicated in our work. The origin of clevelandellid ciliates as well as their possible evolutionary history was also discussed here; however, the analysis of more species from other vertebrate hosts (fish, reptiles) should be made before a well-supported conclusion can be drawn.
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Ming
M
Li
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.
Zong-Yi
ZY
Sun
Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, 86011, USA.
J Norman
JN
Grim
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, Madrid, 28040, Spain. pponce@ucm.es.
Francisco
F
Ponce-Gordo
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Gui-Tang
GT
Wang
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Hong
H
Zou
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Wen-Xiang
WX
Li
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Shan-Gong
SG
Wu
D000818:Animals; D001001:Anura / Q000469:parasitology*; D016798:Ciliophora / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D010802:Phylogeny
D016054:DNA, Protozoan; D004275:DNA, Ribosomal

Nyctotheroides
; Light microscopy; SSU-rDNA; phylogenetic analysis