Rutin ameliorates cisplatin-induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats.
Cisplatin (CP) treatment causes damage in the male reproductive system. Rutin (RUT) is a naturally occurring flavonoid glycoside that has antioxidant and anti-inflammatory properties. This study aimed to investigate effects of RUT against cisplatin-induced reproductive toxicity in male rats. Twenty-one adult male Sprague Dawley rats were used. The control group received physiological saline with oral gavage during 14 days, and physiological saline was injected intraperitoneally (IP) in 10th days of study. CP Group received physiological saline during 14 days, and 10 mg kg-1CP was injected IP in 10th day. RUT + CP group received RUT (150 mg kg-1) during 14 days, and 10 mg kg-1CP was injected IP in 10th day. Spermatological parameters (including motility, cauda epididymal sperm density, dead sperm percentage and morphological sperm abnormalities), biochemical (MDA, GSH, GSH-px, SOD and CAT), histological (H&E dye) and immunochemistry evaluations of testicles were evaluated. CP treatment caused damage on some spermatological parameters, increased the oxidative stress and induced testicular degeneration and apoptosis when compared to the control group. However, RUT treatment mitigates these side effects when compared to the CP alone group. IT is concluded that RUT treatment may reduce CP-induced reproductive toxicity as a potential antioxidant compound.
Department of Reproduction and Artificial Insemination, Veterinary Medicine Faculty, Atatürk University, Erzurum, Turkey.
E H
EH
Aksu
Department of Biochemistry, Veterinary Medicine Faculty, Atatürk University, Erzurum, Turkey.
F M
FM
Kandemir
Department of Pathology, Veterinary Medicine Faculty, Atatürk University, Erzurum, Turkey.
M
M
Özkaraca
Department of Reproduction and Artificial Insemination, Veterinary Medicine Faculty, Atatürk University, Erzurum, Turkey.
A D
AD
Ömür
Department of Biochemistry, Veterinary Medicine Faculty, Atatürk University, Erzurum, Turkey.
S
S
Küçükler
Department of Pathology, Veterinary Medicine Faculty, Atatürk University, Erzurum, Turkey.
S
S
Çomaklı
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012431:Rutin / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
D012431:Rutin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002945:Cisplatin
Apoptosis; cisplatin; male fertility; oxidative stress; rutin