Growthcurver: an R package for obtaining interpretable metrics from microbial growth curves.
BACKGROUND
Plate readers can measure the growth curves of many microbial strains in a high-throughput fashion. The hundreds of absorbance readings collected simultaneously for hundreds of samples create technical hurdles for data analysis.


RESULTS
Growthcurver summarizes the growth characteristics of microbial growth curve experiments conducted in a plate reader. The data are fitted to a standard form of the logistic equation, and the parameters have clear interpretations on population-level characteristics, like doubling time, carrying capacity, and growth rate.


CONCLUSIONS
Growthcurver is an easy-to-use R package available for installation from the Comprehensive R Archive Network (CRAN). The source code is available under the GNU General Public License and can be obtained from Github (Sprouffske K, Growthcurver sourcecode, 2016).
Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, Zurich, 8057, Switzerland.|Swiss Institute of Bioinformatics, Quartier Sorge - Batiment Genopode, Lausanne, 1015, Switzerland.
Kathleen
K
Sprouffske
Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, Zurich, 8057, Switzerland. andreas.wagner@ieu.uzh.ch.|Swiss Institute of Bioinformatics, Quartier Sorge - Batiment Genopode, Lausanne, 1015, Switzerland. andreas.wagner@ieu.uzh.ch.|The Santa Fe Institute, 1399 Hyde Park Road, Santa Fe, New Mexico, 24105, USA. andreas.wagner@ieu.uzh.ch.
Andreas
A
Wagner
D001419:Bacteria / Q000254:growth & development*; D019295:Computational Biology; D016208:Databases, Factual; D016015:Logistic Models; D011381:Programming Languages; D012984:Software
Experimental evolution; Growth curve; Logistic