Recyclable Escherichia coli-Specific-Killing AuNP-Polymer (ESKAP) Nanocomposites.
Escherichia coli plays a crucial role in various inflammatory diseases and infections that pose significant threats to both human health and the global environment. Specifically inhibiting the growth of pathogenic E. coli is of great and urgent concern. By modifying gold nanoparticles (AuNPs) with both poly[2-(methacrylamido)glucopyranose] (pMAG) and poly[2-(methacryloyloxy)ethyl trimethylammonium iodide] (pMETAI), a novel recyclable E. coli-specific-killing AuNP-polymer (ESKAP) nanocomposite is proposed in this study, which based on both the high affinity of glycopolymers toward E. coli pili and the merits of antibacterial quaternized polymers attached to gold nanoparticles. The properties of nanocomposites with different ratios of pMAG to pMETAI grafted onto AuNPs are studied. With a pMAG:pMETAI feed ratio of 1:3, the nanocomposite appeared to specifically adhere to E. coli and highly inhibit the bacterial cells. After addition of mannose, which possesses higher affinity for the lectin on bacterial pili and has a competitive advantage over pMAG for adhesion to pili, the nanocomposite was able to escape from dead E. coli cells, becoming available for repeat use. The recycled nanocomposite retained good antibacterial activity for at least three cycles. Thus, this novel ESKAP nanocomposite is a promising, highly effective, and readily recyclable antibacterial agent that specifically kills E. coli. This nanocomposite has potential applications in biological sensing, biomedical diagnostics, biomedical imaging, drug delivery, and therapeutics.
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Yuqi
Y
Yuan
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Feng
F
Liu
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Lulu
L
Xue
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Hongwei
H
Wang
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Jingjing
J
Pan
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Yuecheng
Y
Cui
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Hong
H
Chen
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering, and Materials Science, Soochow University , Suzhou 215123, People's Republic of China.
Lin
L
Yuan
D000900:Anti-Bacterial Agents; D004926:Escherichia coli; D010861:Fimbriae, Bacterial; D006801:Humans; D053768:Metal Nanoparticles; D053761:Nanocomposites; D011108:Polymers
D000900:Anti-Bacterial Agents; D011108:Polymers
Escherichia coli; antibacterial polymer; glycopolymer; gold nanoparticle; specific-killing