Bioreactor Expansion of Skin-Derived Precursor Schwann Cells.
Scaling up the production of cells in a culture process is a critical step when trying to develop cell-based regenerative therapies. Static cultures often cannot be easily scaled up to clinically relevant cell numbers. Alternatively, bioreactors offer a highly valuable means to develop a clinical-ready process. To culture adherent cells in suspension, such as skin-derived precursor Schwann cells (SKP-SCs), microcarriers need to be used. Microcarriers are small spherical beads suspended within the vessel that allow for higher growth surface area to volume ratio. Here we describe the procedure of combining microcarriers with the controllability of bioreactors to generate higher cell densities in smaller reactor volumes leading to a more efficient and cost-effective cell production for applications in regenerative medicine.
Pharmaceutical Production Research Facility (PPRF), University of Calgary, 2500 University Drive NW, Calgary, AB, Canada, T2N 1N4.|Biomedical Engineering Graduate Program, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada, T2N 1N4.
Tylor
T
Walsh
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, Alberta Children's Hospital Research Institute, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, Canada, T2N 4N1.|Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, 2500 University Drive NW, Calgary, AB, Canada, T2N 1N4.
Jeff
J
Biernaskie
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, Alberta Children's Hospital Research Institute, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, Canada, T2N 4N1.
Rajiv
R
Midha
Pharmaceutical Production Research Facility (PPRF), University of Calgary, 2500 University Drive NW, Calgary, AB, Canada, T2N 1N4. mskallos@ucalgary.ca.|Biomedical Engineering Graduate Program, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada, T2N 1N4. mskallos@ucalgary.ca.|Chemical and Petroleum Engineering, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada, T2N 1N4. mskallos@ucalgary.ca.
Michael S
MS
Kallos
D019149:Bioreactors / Q000191:economics; D002452:Cell Count; D018929:Cell Culture Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D044968:Regenerative Medicine; D012583:Schwann Cells / Q000166:cytology*; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D013242:Sterilization / Q000379:methods
Bioreactors; Microcarriers; Regenerative medicine; SKP-SCs; Scale-up; Schwann cells