Microbial population dynamics during sludge granulation in an A/O/A sequencing batch reactor.
The evolution of the bacterial population during formation of denitrifying phosphorus removal granular sludge was investigated using high-throughput pyrosequencing. As a result, mature granules with a compact structure were obtained in an anaerobic/aerobic/anoxic (A/O/A) sequencing batch reactor under an organic loading rate as low as 0.3kg COD/(m(3)·d). Rod-shaped microbes were observed to cover with the outer surface of granules. Besides, reliable COD and simultaneous nitrogen and phosphorus removal efficiencies were achieved over the whole operation period. MiSeq pyrosequencing analysis illustrated that both the microbial diversity and richness increased sharply during the granulation process, whereas they stayed stable after the presence of granules. Some microorganisms seemed to contribute to the formation of granules, and some were identified as functional bacterial groups responsible for constructing the biological reactor.
School of Civil Engineering, Wuhan University, Wuhan 430072, China.
Qiulai
Q
He
School of Civil Engineering, Wuhan University, Wuhan 430072, China.
Jun
J
Zhou
School of Civil Engineering, Wuhan University, Wuhan 430072, China. Electronic address: hywang96@126.com.
Hongyu
H
Wang
School of Civil Engineering, Wuhan University, Wuhan 430072, China.
Jing
J
Zhang
State Key Lab of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150090, China.
Li
L
Wei
D000332:Aerobiosis; D000693:Anaerobiosis; D041963:Bacteroidetes / Q000235:genetics; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D041862:Chloroflexi / Q000235:genetics; D000068536:Bacillota / Q000235:genetics; D059013:Microbial Consortia; D056265:Microbial Interactions; D058889:Molecular Typing; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D010802:Phylogeny; D020560:Proteobacteria / Q000235:genetics; D012722:Sewage / Q000032:analysis / Q000382:microbiology*
D012722:Sewage; D010758:Phosphorus; D009584:Nitrogen
A/O/A; Denitrifying phosphorus removal; Granular sludge; MiSeq pyrosequencing; Microbial population