[Phenotypic modulation of bladder smooth muscle in diabetic rats].
OBJECTIVE
To investigate whether phenotypic modulation of bladder smooth muscle occurs in diabetic rats.


METHODS
Thirty-two male SD rats were randomly assigned into diabetic group and control group. Diabetic rat models were established by a single intraperitoneal injection of streptozotocin (60 mg/kg). Nine weeks later, the bladder tissues of the rats were examined for structural changes using HE and Masson's trichrome staining , and the expressions of myocardin, α-SMA, and SMMHC in bladder smooth muscles were detected with RT-PCR and Western blotting.


RESULTS
Compared with the control group, the diabetic rats showed obvious polydipsia and polyuria with significantly increased collagenous fibers and lowered expressions of myocardin, α-SMA, and SMMHC in the bladder tissue (P<0.05).


CONCLUSIONS
s In rats at 9 weeks after diabetic model establishment, phenotypic transition of the bladder smooth muscles occurs to cause bladder contractile dysfunction, which may play an important role in the pathology of diabetic bladder dysfunction.
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail: liangyanbing2013@163.com.
Yan-Bing
YB
Liang
An-Yang
AY
Wei
Tao
T
Wang
Shu-Hua
SH
He
Hai-Bo
HB
Zhang
Ze-Rong
ZR
Chen
Feng-Zhi
FZ
Chen
Zhi-Qiang
ZQ
Wang
D000199:Actins / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D008297:Male; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000503:physiopathology*; D018995:Myosin Heavy Chains / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D015534:Trans-Activators / Q000378:metabolism; D001743:Urinary Bladder / Q000503:physiopathology*; D000099251:Myocardin
D000199:Actins; D018995:Myosin Heavy Chains; D009687:Nuclear Proteins; D013311:Streptozocin; D015534:Trans-Activators; D000099251:Myocardin; C000595077:Myh11 protein, rat; C498862:Acta2 protein, rat