Methods to Study Plant Programmed Cell Death.
Programmed cell death (PCD) is a critical component of plant development, defense against invading pathogens, and response to environmental stresses. In this chapter, we provide detailed technical methods for studying PCD associated with plant development or induced by abiotic stress. A root hair assay or electrolyte leakage assay are excellent techniques for the quantitative determination of PCD and/or cellular injury induced in response to abiotic stress, whereas the lace plant provides a unique model that facilitates the study of genetically regulated PCD during leaf development.
School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.
Joanna
J
Kacprzyk
Department of Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R.
Adrian N
AN
Dauphinee
Faculty of Life Sciences, University of Manchester, D3515 Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK.
Patrick
P
Gallois
Department of Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R.
Arunika Hlan
AH
Gunawardena
School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland. paul.mccabe@ucd.ie.
Paul F
PF
McCabe
D034901:Alismataceae / Q000166:cytology* / Q000502:physiology; D016923:Cell Death; D057054:Molecular Imaging / Q000379:methods*; D018515:Plant Leaves / Q000166:cytology* / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013312:Stress, Physiological
D010940:Plant Proteins
Abiotic stress; Developmental programmed cell death; Electrolyte leakage; Leaf morphogenesis; Programmed cell death; Root hair assay