Sealing ability of MTA, CPM, and MBPc as root-end filling materials: a bacterial leakage study.
Objectives To evaluate the sealing ability of three root-end filling materials (white MTA, CPM, and MBPc) using an Enterococcus faecalis leakage model. Material and Methods Seventy single-root extracted human teeth were instrumented and root-ends were resected to prepare 3 mm depth cavities. Root-end preparations were filled with white MTA, CPM, and MBPc cements. Enterococcus faecalis was coronally introduced and the apical portion was immersed in BHI culture medium with phenol red indicator. The bacterial leakage was monitored every 24 h for 4 weeks. The statistical analysis was performed using the Wilcoxon-Gehan test (p<0.05). Results All cements showed bacterial leakage after 24 hours, except for the negative control group. The MBPc showed significantly less bacterial leakage compared with the MTA group (p<0.05). No significant differences were found between the CPM and the other groups. Conclusions The epoxy resin-based cement MBPc had lower bacterial leakage compared with the calcium silicate-based cements MTA and CPM.
Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
Paulo Leal
PL
Medeiros
Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
Norberti
N
Bernardineli
Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
Bruno Cavalini
BC
Cavenago
Departamento de Microbiologia e Imunologia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
Sérgio Aparecido
SA
Torres
Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
Marco Antonio Hungaro
MA
Duarte
Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
Clovis Monteiro
CM
Bramante
Departamento de Dentística, Materiais Odontológicos e Endodontia, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil.
Marina Angélica
MA
Marciano
D017607:Aluminum Compounds / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D003738:Dental Cements / Q000737:chemistry*; D003763:Dental Leakage / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis; D006801:Humans; D008422:Materials Testing; D010087:Oxides / Q000737:chemistry*; D015203:Reproducibility of Results; D012184:Retrograde Obturation / Q000379:methods; D012387:Root Canal Filling Materials / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D018709:Statistics, Nonparametric; D013997:Time Factors; D014092:Tooth Root / Q000187:drug effects / Q000382:microbiology
D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate