Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone.
Naringin maintains bone mass in various osteoporosis models, while its effect on bone in disuse osteoporosis has not been reported. The present study explores whether naringin can prevent disuse osteoporosis induced by unilateral sciatic neurectomy (USN) and whether the Semaphorin 3A-induced Wnt/β-catenin signalling pathway is involved in the osteoprotection of naringin. Naringin dose-dependently prevented the deterioration of bone mineral density (BMD), trabecular structure and biomechanical strength in femur due to USN. Naringin increased bone formation but inhibited resorption, as indicated by bone-turnover markers in blood and urine and the histological staining of Osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP) in femur. Semaphorin 3A (Sema3A) and active β-catenin protein decreased after USN and could be restored by naringin to the levels of the sham-operated rats. In addition, naringin in vitro promoted the differentiation of osteoblasts and inhibited osteoclastic differentiation. Our studies suggest that the down-regulation of Sema3A and the subsequent inactivation of Wnt/β-catenin signalling may be some of the mechanisms involved in USN-induced osteoporosis. Naringin could increase the expression of Sema3A and the activation of Wnt/β-catenin signalling to prevent disuse osteoporosis induced by denervation. Thus, naringin functions in bone maintenance and could be a promising therapeutic alternative in preventing disuse osteoporosis.
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.|Tianjin Institute of Orthopaedics in Traditional Chinese and Western Medicine, No. 122, Munan Road, Tianjin TJ 300050, China.|Graduate School of Tianjin Medical University, No. 22, Qixiangtai Street, Heping District, Tianjin TJ 300070, China.
Xinlong
X
Ma
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.|Tianjin Institute of Orthopaedics in Traditional Chinese and Western Medicine, No. 122, Munan Road, Tianjin TJ 300050, China.|Graduate School of Tianjin Medical University, No. 22, Qixiangtai Street, Heping District, Tianjin TJ 300070, China.
Jianwei
J
Lv
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.|Tianjin Institute of Orthopaedics in Traditional Chinese and Western Medicine, No. 122, Munan Road, Tianjin TJ 300050, China.
Xiaolei
X
Sun
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.|Tianjin Institute of Orthopaedics in Traditional Chinese and Western Medicine, No. 122, Munan Road, Tianjin TJ 300050, China.
Jianxiong
J
Ma
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.
Guosheng
G
Xing
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.
Ying
Y
Wang
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.
Lei
L
Sun
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.
Jianbao
J
Wang
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.
Fengbo
F
Li
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.
Yanjun
Y
Li
Institute of Orthopaedics, Tianjin Hospital, No. 122, Munan Road, Heping District, Tianjin TJ 300050, China.|Tianjin Institute of Orthopaedics in Traditional Chinese and Western Medicine, No. 122, Munan Road, Tianjin TJ 300050, China.
Zhihu
Z
Zhao
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003714:Denervation; D005269:Femur / Q000378:metabolism / Q000473:pathology*; D044950:Flavanones / Q000627:therapeutic use*; D008297:Male; D015675:Osteocalcin / Q000378:metabolism; D010024:Osteoporosis / Q000209:etiology / Q000517:prevention & control*; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000473:pathology* / Q000601:surgery; D040121:Semaphorin-3A / Q000235:genetics / Q000378:metabolism*; D000071681:Tartrate-Resistant Acid Phosphatase / Q000378:metabolism; D015854:Up-Regulation; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism
D044950:Flavanones; D040121:Semaphorin-3A; D051176:beta Catenin; D015675:Osteocalcin; D000071681:Tartrate-Resistant Acid Phosphatase; C005274:naringin