Rapid identification of microorganisms from positive blood cultures by testing early growth on solid media using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
We performed a retrospective analysis of a simple modification to MALDI-TOF MS for microorganism identification to accurately improve the turnaround time (TAT) for identification of Enterobacteriaceae recovered in blood cultures. Relative to standard MALDI-TOF MS procedures, we reduced TAT from 28.3 (n=90) to 21.2h (n=107).
Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Mark D
MD
Gonzalez
Department of Laboratories, Barnes-Jewish Hospital, St. Louis, MO, USA.
Carol J
CJ
Weber
Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: cburnham@path.wustl.edu.
Carey-Ann D
CA
Burnham
D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D000071997:Blood Culture; D003470:Culture Media; D004755:Enterobacteriaceae / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D012189:Retrospective Studies; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
D003470:Culture Media
Anaerobes; Blood cultures; Enterobacteriaceae; MALDI-TOF MS; Non-fermenters; Yeast