Gene expression profiling of normal thyroid tissue from patients with thyroid carcinoma.
Gene expression profiling (GEP) of normal thyroid tissue from 43 patients with thyroid carcinoma, 6 with thyroid adenoma, 42 with multinodular goiter, and 6 with Graves-Basedow disease was carried out with the aim of achieving a better understanding of the genetic mechanisms underlying the role of normal cells surrounding the tumor in the thyroid cancer progression. Unsupervised and supervised analyses were performed to compare samples from neoplastic and non-neoplastic diseases. GEP and subsequent RT-PCR analysis identified 28 differentially expressed genes. Functional assessment revealed that they are involved in tumorigenesis and cancer progression. The distinct GEP is likely to reflect the onset and/or progression of thyroid cancer, its molecular classification, and the identification of new potential prognostic factors, thus allowing to pinpoint selective gene targets with the aim of realizing more precise preoperative diagnostic procedures and novel therapeutic approaches.This study is focused on the gene expression profiling analysis followed by RT-PCR of normal thyroid tissues from patients with neoplastic and non-neoplastic thyroid diseases. Twenty-eight genes were found to be differentially expressed in normal cells surrounding the tumor in the thyroid cancer. The genes dysregulated in normal tissue samples from patients with thyroid tumors may represent new molecular markers, useful for their diagnostic, prognostic and possibly therapeutic implications.
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine "G. Baccelli", Bari, BA, Italy.
Roberto
R
Ria
Laboratory of Pre-Clinical and Traslational Research, IRCSS CROB, Rionero in Vulture, PZ, Italy.
Vittorio
V
Simeon
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine "G. Baccelli", Bari, BA, Italy.
Assunta
A
Melaccio
Department of Biomedical Sciences and Human Oncology, Unit of Endocrine, Digestive and Emergency Surgery, Bari, BA, Italy.
Giovanna
G
Di Meo
Laboratory of Pre-Clinical and Traslational Research, IRCSS CROB, Rionero in Vulture, PZ, Italy.
Stefania
S
Trino
Laboratory of Pre-Clinical and Traslational Research, IRCSS CROB, Rionero in Vulture, PZ, Italy.
Carmela
C
Mazzoccoli
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine "G. Baccelli", Bari, BA, Italy.
Ilaria
I
Saltarella
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine "G. Baccelli", Bari, BA, Italy.
Aurelia
A
Lamanuzzi
Laboratory of Pre-Clinical and Traslational Research, IRCSS CROB, Rionero in Vulture, PZ, Italy.
Annalisa
A
Morano
Department of Biomedical Sciences and Human Oncology, Unit of Endocrine, Digestive and Emergency Surgery, Bari, BA, Italy.
Angela
A
Gurrado
Department of Biomedical Sciences and Human Oncology, Unit of Endocrine, Digestive and Emergency Surgery, Bari, BA, Italy.
Alessandro
A
Pasculli
Department of Pathology, University of Bari "Aldo Moro" Medical School, Bari, BA, Italy.
Gaetano
G
Lastilla
Laboratory of Pre-Clinical and Traslational Research, IRCSS CROB, Rionero in Vulture, PZ, Italy.
Pellegrino
P
Musto
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine "G. Baccelli", Bari, BA, Italy.
Antonia
A
Reale
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine "G. Baccelli", Bari, BA, Italy.
Franco
F
Dammacco
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine "G. Baccelli", Bari, BA, Italy.
Angelo
A
Vacca
Department of Biomedical Sciences and Human Oncology, Unit of Endocrine, Digestive and Emergency Surgery, Bari, BA, Italy.
Mario
M
Testini
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D013961:Thyroid Gland / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000235:genetics*; D059467:Transcriptome
D014408:Biomarkers, Tumor
gene expression profile; hypoxia; microenvironment; oncogenes; thyroid cancer