Capillary Electrophoresis-Mass Spectrometry for Clinical Metabolomics.
In clinical metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) has become a very useful technique for the analysis of highly polar and charged metabolites in complex biologic samples. A comprehensive overview of recent developments in CE-MS for metabolic profiling studies is presented. This review covers theory, CE separation modes, capillary coatings, and practical aspects of CE-MS coupling. Attention is also given to sample pretreatment and data analysis strategies used for metabolomics. The applicability of CE-MS for clinical metabolomics is illustrated using samples ranging from plasma and urine to cells and tissues. CE-MS application to large-scale and quantitative clinical metabolomics is addressed. Conclusions and perspectives on this unique analytic strategy are presented.
Leiden University, Leiden, The Netherlands. Electronic address: r.ramautar@lacdr.leidenuniv.nl.
Rawi
R
Ramautar
D002624:Chemistry, Clinical; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*
Blood; Capillary electrophoresis; Cerebrospinal fluid; Data analysis; Mass spectrometry; Metabolomics; Sample pretreatment; Urine