Optimizing Colorimetric Assay Based on V₂O₅ Nanozymes for Sensitive Detection of H₂O₂ and Glucose.
Nanozyme-based chemical sensing is a rapidly emerging field of research. Herein, a simple colorimetric assay for the detection of hydrogen peroxide and glucose based on the peroxidase-like activity of V₂O₅ nanozymes has been established. In this assay, the effects of pH, substrate, nanozyme concentrations and buffer solution have been investigated. It was found that compared with 3,3',5,5'-tetramethylbenzidine (TMB), the enzyme substrate o-phenylenediamine (OPD) seriously interfered with the H₂O₂ detection. Under the optimal reaction conditions, the resulting sensor displayed a good response to H₂O₂ with a linear range of 1 to 500 μM, and a detection limit of 1 μM at a signal-to-noise ratio of 3. A linear correlation was established between absorbance intensity and concentration of glucose from 10 to 2000 μM, with a detection limit of 10 μM. The current work presents a simple, cheap, more convenient, sensitive, and easy handling colorimetric assay.
School of Public Health, Jilin University, Changchun 130021, China. sunjh15@mails.jlu.edu.cn.
Jiaheng
J
Sun
School of Public Health, Jilin University, Changchun 130021, China. licy14@mails.jlu.edu.cn.
Chunyan
C
Li
School of Public Health, Jilin University, Changchun 130021, China. qiyanfei@jlu.edu.cn.
Yanfei
Y
Qi
School of Public Health, Jilin University, Changchun 130021, China. guosl15@mails.jlu.edu.cn.
Shuanli
S
Guo
School of Public Health, Jilin University, Changchun 130021, China. liangxue2714@mails.jlu.edu.cn.
Xue
X
Liang
D003124:Colorimetry; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000032:analysis*; D009195:Peroxidase; D017968:Vanadium Compounds
D017968:Vanadium Compounds; D006861:Hydrogen Peroxide; C066075:vanadium pentoxide; D009195:Peroxidase; D005947:Glucose
H2O2; V2O5 nanozymes; glucose; substrates; variation