Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide.
To obtain lactic acid bacteria that scavenge environmental hydrogen peroxide, we developed a specialized enrichment medium and successfully isolated Pediococcus pentosaceus Be1 strain from a fermented food. This strain showed vigorous environmental hydrogen peroxide scavenging activity over a wide range of hydrogen peroxide concentrations. High Mn-catalase and NADH peroxidase activities were found in the cell-free extract of the P. pentosaceus Be1 strain, and these two hydrogen peroxide scavenging enzymes were purified from the cell-free extract of the strain. Mn-catalase has been purified from several microorganisms by several researchers, and the NADH peroxidase was first purified from the original strain in this report. After cloning the genes of the Mn-catalase and the NADH peroxidase, the deduced amino acid sequences were compared with those of known related enzymes.
Department of Bio-Science, Tokyo University of Agriculture.
Akio
A
Watanabe
Chiaki
C
Kaneko
Yasuhiro
Y
Hamada
Kouji
K
Takeda
Shinya
S
Kimata
Takashi
T
Matsumoto
Akira
A
Abe
Naoto
N
Tanaka
Sanae
S
Okada
Masataka
M
Uchino
Junichi
J
Satoh
Junichi
J
Nakagawa
Youichi
Y
Niimura
D000595:Amino Acid Sequence; D002374:Catalase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005516:Food Microbiology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology; D010084:Oxidation-Reduction; D000070006:Pediococcus pentosaceus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D031224:Raphanus / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology
D003470:Culture Media; D006861:Hydrogen Peroxide; D010544:Peroxidases; C021845:NAD+ peroxidase; D002374:Catalase