Exogenous 3,3'-diindolylmethane increases Brassica napus L. seedling shoot growth through modulation of superoxide and hydrogen peroxide content.
Brassica napus L. (cv. AV Garnet) seeds were pre-treated with 15μM 3,3'-diindolylmethane (DIM) to investigate whether DIM could enhance seed germination. Further treatment of seedlings with 15μM DIM for 14days explored the effects on seedling shoot growth. Exogenous DIM led to improved germination percentage, increased seedling shoot lengths, and increased fresh and dry weights. Furthermore, DIM triggered induction of superoxide radical (O2(-)) and hydrogen peroxide (H2O2) content however, no change in malondialdehyde (MDA) content and cell death (assessed with Evans Blue assay) was detected for both the control and DIM treated seedling shoots. We also observed increases in superoxide dismutase (SOD) activity and ascorbate peroxidase (APX) activity in response to exogenous DIM, two fundamental enzymes in the control of reactive oxygen species (ROS) in plants. These results indicate that exogenous DIM treatment enhances seed germination and improves seedling shoot growth through possible activation of a reactive oxygen species signalling pathway involving O2(-) and H2O2 in B. napus.
Environmental Biotechnology Laboratory, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
Arun
A
Gokul
Environmental Biotechnology Laboratory, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
Enrico
E
Roode
Proteomics Research Unit, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
Ashwil
A
Klein
Environmental Biotechnology Laboratory, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa. Electronic address: mkeyster@uwc.ac.za.
Marshall
M
Keyster
D000975:Antioxidants / Q000378:metabolism*; D029688:Brassica napus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D018525:Germination / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D007211:Indoles; D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide; C016392:3,3'-diindolylmethane
3,3′-Diindolylmethane; Brassica napus; Cell death; Hydrogen peroxide; Reactive oxygen species; Superoxide radical