Investigation on Immunomodulatory Activity of Calf Spleen Extractive Injection in Cyclophosphamide-induced Immunosuppressed Mice and Underlying Mechanisms.
Calf spleen extractive injection (CSEI), extracted from the spleen of healthy cows (within 24 h of birth), is a small peptides enriched extraction while the ratio between peptide and ribose is 76 ± 15.2 mg/μg. CSEI is usually used as an ancillary agent to assist cancer patients with immune dysfunction. The present study aims to evaluate the immunomodulatory activity of CSEI in cyclophosphamide (CTX)-induced mice model of immunosuppression and its underlying mechanisms. During the experiment, thymosin ɑ1 (0.16 mg/kg) was served as the positive control drug. In CTX-induced immunosuppressed mice, CSEI significantly increased bodyweights and spleen indexes, and upgraded the natural killer activity together with lymphocytes proliferation. CSEI regulated the production of IgG and IgA, and the levels of IL-2, 6, 10, 12 and IFN-ɑ, γ and TNF-ɑ in serum of CTX-induced immunosuppressed mice. Furthermore, CSEI markedly downregulated nuclear factor kappa-B (NF-κB) expression which was controlled by IKKβ. Taken together, CSEI effectively improved immune function in CTX-induced immunosuppression related to NF-κB signalling pathway via regulating the production of immunoglobulins, interleukins and some inflammatory factors.
School of Life Sciences, Jilin University, Changchun, China.
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Jia
School of Life Sciences, Jilin University, Changchun, China.
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Lu
School of Life Sciences, Jilin University, Changchun, China.
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Wang
JiLin AoDong Medicine Industry Group Co. Ltd., Taonan, China.
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Sun
School of Life Sciences, Jilin University, Changchun, China.
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Cai
School of Life Sciences, Jilin University, Changchun, China.
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Li
JiLin AoDong Medicine Industry Group Co. Ltd., Taonan, China.
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Wang
School of Life Sciences, Jilin University, Changchun, China.
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Liu
School of Life Sciences, Jilin University, Changchun, China.
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Zhang
School of Life Sciences, Jilin University, Changchun, China.
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Wang
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002457:Cell Extracts / Q000627:therapeutic use*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003520:Cyclophosphamide / Q000008:administration & dosage; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D007165:Immunosuppression Therapy; D007167:Immunotherapy / Q000379:methods*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D013154:Spleen / Q000378:metabolism
D002457:Cell Extracts; D016207:Cytokines; D016328:NF-kappa B; D003520:Cyclophosphamide