Oxygen-Free Condition Inhibited Biofilm Formation in Extraintestinal Pathogenic Escherichia coli Strain PPECC42 Through Preventing Curli Production.
Extraintestinal pathogenic Escherichia coli (ExPEC) is an important zoonotic and foodborne pathogen. Biofilms are specially structured communities for bacteria to survive in different hostile environments and can protect the bacteria from eradication by the host and external factors. In this study, we found that oxygen is definitely required for biofilm formation in ExPEC strain PPECC42. Aerobically growing ExPEC showed a bdar (brown, dry, and rough) morphotype, whereas anaerobically growing ExPEC showed a saw (smooth and white) morphotype. Under anaerobic condition, curli fimbriae did not accumulate and the expression levels of curli biosynthesis-related genes including csgB, csgD, and rpoS decreased significantly; in contrast, the expression level of h-ns, of which the encoding protein is a repressor for csgD transcription, increased significantly. Taken together, the results suggested that oxygen-free condition limited ExPEC strain PPECC42 biofilm formation mainly through preventing curli accumulation by affecting the transcriptional levels of curli biosynthesis-related genes.
State Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street No 1, Wuhan, 430070, People's Republic of China.
Xianrong
X
Meng
State Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street No 1, Wuhan, 430070, People's Republic of China.
Liyuan
L
Zhang
State Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street No 1, Wuhan, 430070, People's Republic of China.
Bo
B
Hou
State Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street No 1, Wuhan, 430070, People's Republic of China.
Xueling
X
Liu
State Key Lab of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street No 1, Wuhan, 430070, People's Republic of China. lishaowen@mail.hzau.edu.cn.
Shaowen
S
Li
D000818:Animals; D018441:Biofilms; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D000072282:Extraintestinal Pathogenic Escherichia coli / Q000235:genetics / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008168:Lung / Q000382:microbiology; D010100:Oxygen / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D029968:Escherichia coli Proteins; D010100:Oxygen