MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities.
Mastoparans from the venom of social wasps have attracted considerable attention as effective antibiotic candidates. In this study, mastoparan V1 (MP-V1) from Vespula vulgaris was first disclosed to have a peptide amino acid sequence distinct from typical mastoparans and its biochemical properties and antimicrobial effects were compared with those of typical mastoparans MP-L, -X(V) and -B. Circular dichroism (CD) spectroscopy revealed that MP-V1 and -X(V) form more stable α-helical conformations in lipid membrane-like environments than MP-L and -B. In parallel, these two also showed more effective antimicrobial activities against the pathogens than did MP-L and -B. Although MP-V1 had a less stable α-helical conformation than MP-X(V), it showed stronger antimicrobial effects against Streptococcus mutans and Salmonella enterica than MP-X(V). In the meantime, analysis of hemolytic activity revealed a range of doses (~50 μM) that exhibited little potent cytotoxicity on human erythrocytes. Finally, the atypical MP-V1 peptide amino acid sequence provided important clues to understanding its antimicrobial mechanism from a structural perspective. Therefore, it has been concluded that MP-V1 is a de novo type of mastoparan with superior antimicrobial activities against both pathogenic bacteria and fungi, which may be useful in developing multipurpose antimicrobial drugs against infectious diseases.
National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, Korea. nimitzdr93@gmail.com.
Yangseon
Y
Kim
Division of Applied Life Science (BK21 Plus) &amp; Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University (GNU), 501 Jinju-daero, Jinju 660-701, Korea. minky@gnu.ac.kr.
Minky
M
Son
National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, Korea. rey0426@korea.kr.
Eun-Young
EY
Noh
National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, Korea. sokim90@korea.kr.
Soonok
S
Kim
National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, Korea. snubull@korea.kr.
Changmu
C
Kim
National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, Korea. y1208@korea.kr.
Joo-Hong
JH
Yeo
Division of Applied Life Science (BK21 Plus) &amp; Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University (GNU), 501 Jinju-daero, Jinju 660-701, Korea. chaninpark0806@gmail.com.
Chanin
C
Park
Division of Applied Life Science (BK21 Plus) &amp; Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University (GNU), 501 Jinju-daero, Jinju 660-701, Korea. kwlee@gnu.ac.kr.
Keun Woo
KW
Lee
National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, Korea. wybang@korea.kr.
Woo Young
WY
Bang
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D002942:Circular Dichroism; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017433:Protein Structure, Secondary; D019779:Salmonella enterica / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014862:Wasp Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D014863:Wasps / Q000737:chemistry / Q000378:metabolism*
D000890:Anti-Infective Agents; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014862:Wasp Venoms; C037500:mastoparan
MP-V1; antimicrobial activity; mastoparan; peptide; venom; wasp