Effect of heterogeneous Fenton-like pre-treatment on anaerobic granular sludge performance and microbial community for the treatment of traditional Chinese medicine wastewater.
The effect of a heterogeneous Fenton-like pre-treatment on the anaerobic processes, characteristics and microbial community of sludge was investigated for traditional Chinese medicine (TCM) wastewater containing rhein. When the concentrations of rhein were 50mg/L and 100mg/L, the toxic effect was physiological toxicity for anaerobic granular sludge. Using a single double circle (DC) reactor for the treatment of TCM wastewater containing rhein at concentrations of 15-20mg/L, the chemical oxygen demand (COD) removal rate was 69%, and coenzyme F420 was nearly undetectable in the 3D-excitation-emission matrix (EEM) spectra of soluble microbial products (SMP). The abundances of Methanoregula, Methanobacterium, Methanosphaerula were only 5.57%, 2.39% and 1.08% in the DC reactor, respectively. TCM wastewater containing rhein could be successfully treated by the combination of the heterogeneous Fenton-like pre-treatment and the DC reactor processes, and the COD removal rate reached 95%. Meanwhile, the abundances of Methanoregula, Methanobacterium, Methanosphaerula increased to 22.5%, 18.5%, and 13.87%, respectively. For the bacterial community, the abundance of Acidobacteria_Gp6 decreased from 6.99% to 1.07%, while the abundances of Acidobacteria_Gp1 and Acidobacteria_Gp2 increased from 1.61% to 6.55% and from 1.28% to 5.87%, respectively.
School of Environment and Resources, Guangxi Normal University, 15 Yucai Road, Guilin 541004, PR China; School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China. Electronic address: suchengyuan2008@126.com.
Chengyuan
C
Su
School of Municipal and Environmental Engineering, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China.
Weiguang
W
Li
School of Environment and Resources, Guangxi Normal University, 15 Yucai Road, Guilin 541004, PR China.
Yuxiang
Y
Lu
School of Environment and Resources, Guangxi Normal University, 15 Yucai Road, Guilin 541004, PR China.
Menglin
M
Chen
School of Environment and Resources, Guangxi Normal University, 15 Yucai Road, Guilin 541004, PR China.
Zhi
Z
Huang
D000693:Anaerobiosis; D000880:Anthraquinones; D019149:Bioreactors; D008516:Medicine, Chinese Traditional; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000382:microbiology*
D000880:Anthraquinones; D012722:Sewage; D062065:Wastewater; C020491:rhein
Anaerobic granular sludge; Heterogeneous fenton-like; Microbial community dynamics; Pre-treatment; Toxicity assessment