Regulation of arcuate genes by developmental exposures to endocrine-disrupting compounds in female rats.
Developmental exposure to endocrine-disrupting compounds (EDCs) alters reproduction and energy homeostasis, both of which are regulated by the arcuate nucleus (ARC). Little is known about the effects of EDC on ARC gene expression. In Experiment #1, pregnant dams were treated with either two doses of bisphenol A (BPA) or oil from embryonic day (E)18-21. Neonates were injected from postnatal day (PND)0-7. Vaginal opening, body weights, and ARC gene expression were measured. Chrm3 (muscarinic receptor 3) and Adipor1 (adiponectin receptor 1) were decreased by BPA. Bdnf (brain-derived neurotropic factor), Igf1 (insulin-like growth factor 1), Htr2c (5-hydroxytryptamine receptor), and Cck2r (cholescystokinin 2 receptor) were impacted. In Experiment #2, females were exposed to BPA, diethylstilbestrol (DES), di(2-ethylhexyl)phthalate, or methoxychlor (MXC) during E11-PND7. MXC and DES advanced the age of vaginal opening and ARC gene expression was impacted. These data indicate that EDCs alter ARC genes involved in reproduction and energy homeostasis in females.
Department of Animal Sciences, School of Environmental & Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA. Electronic address: ta.roepke@rutgers.edu.
Troy A
TA
Roepke
Department of Animal Sciences, School of Environmental & Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
Jennifer A
JA
Yang
Department of Animal Sciences, School of Environmental & Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
Ali
A
Yasrebi
Department of Animal Sciences, School of Environmental & Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
Kyle J
KJ
Mamounis
Department of Animal Sciences, School of Environmental & Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
Elif
E
Oruc
Department of Animal Sciences, School of Environmental & Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
Aparna Mahakali
AM
Zama
Department of Animal Sciences, School of Environmental & Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
Mehmet
M
Uzumcu
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000633:toxicity; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D004051:Diethylhexyl Phthalate / Q000633:toxicity; D004054:Diethylstilbestrol / Q000633:toxicity; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006706:Homeostasis / Q000187:drug effects; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008731:Methoxychlor / Q000633:toxicity; D010636:Phenols / Q000633:toxicity; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011916:Rats, Inbred F344; D043887:Receptor, Cholecystokinin B / Q000235:genetics; D043587:Receptor, Muscarinic M3 / Q000235:genetics; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics; D054419:Receptors, Adiponectin / Q000235:genetics; D012741:Sexual Maturation / Q000187:drug effects; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D019208:Brain-Derived Neurotrophic Factor; D004051:Diethylhexyl Phthalate; D004054:Diethylstilbestrol; D052244:Endocrine Disruptors; D007334:Insulin-Like Growth Factor I; D008731:Methoxychlor; D010636:Phenols; D043887:Receptor, Cholecystokinin B; D043587:Receptor, Muscarinic M3; D044405:Receptor, Serotonin, 5-HT2C; D054419:Receptors, Adiponectin; C504339:adiponectin receptor 1, rat; C548904:insulin-like growth factor-1, rat; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Arcuate nucleus; Bisphenol a; Diethylstilbestrol; Endocrine-disrupting compounds; Energy homeostasis; Methoxychlor; Phthalates; Puberty