irCLIP platform for efficient characterization of protein-RNA interactions.
The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
Brian J
BJ
Zarnegar
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.|Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
Ryan A
0000-0001-5013-0442
RA
Flynn
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
Ying
Y
Shen
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.|Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
Brian T
BT
Do
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.|Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
Howard Y
HY
Chang
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.|Veterans Affairs, Palo Alto Healthcare System, Palo Alto, California, USA.
Paul A
PA
Khavari
D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D006367:HeLa Cells; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D055668:Photochemical Processes; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D012680:Sensitivity and Specificity; D059467:Transcriptome; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D018076:DNA, Complementary; D016601:RNA-Binding Proteins