Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
A comprehensive characterization of polysaccharides from the chanterelle was performed. Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC. A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography. The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4. Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, N-0316 Oslo, Norway. Electronic address: a.a.t.nyman@farmasi.uio.no.
Anna Armika T
AA
Nyman
Department of Biotechnology, NOBIPOL, Norwegian University of Science and Technology, N-7491 Trondheim, Norway. Electronic address: finn.l.aachmann@ntnu.no.
Finn L
FL
Aachmann
Department of Chemistry, University of Oslo, P.O. Box 1033 Blindern, N-0315 Oslo, Norway. Electronic address: frode.rise@kjemi.uio.no.
Frode
F
Rise
Nofima, Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210, NO-1431 Aas, Norway. Electronic address: Simon.Ballance@Nofima.no.
Simon
S
Ballance
School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, N-0316 Oslo, Norway. Electronic address: a.b.c.samuelsen@farmasi.uio.no.
Anne Berit C
AB
Samuelsen
D000363:Agaricales / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry*; D015394:Molecular Structure; D047071:beta-Glucans / Q000737:chemistry*
D008351:Mannans; D047071:beta-Glucans
Basidiomycota; Chanterelle; Glucan; Mannan; NMR; Saccharomyces cerevisiae