Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection.
Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples.
Department of Internal Medicine, Radboud University Medical Center, Geert Grooteplein-Zuid 10, 6525 GA, Nijmegen, The Netherlands; Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ, Nijmegen, The Netherlands; Hasselt University, Martelarenlaan 42, BE3500, Hasselt, Belgium.
Mark H T
MH
Stappers
Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ, Nijmegen, The Netherlands.
Ferry
F
Hagen
Bayer Healthcare Pharmaceuticals, Friedrich-Ebert-Straße 475, 42117, Wuppertal, Germany.
Peter
P
Reimnitz
Department of Medical Microbiology, Radboud University Medical Center, Geert Grooteplein-Zuid 10, 6525 GA, Nijmegen, The Netherlands; Department of Medical Microbiology and Infectious Diseases, Erasmus MC, 's-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.
Johan W
JW
Mouton
Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ, Nijmegen, The Netherlands; Department of Medical Microbiology, Radboud University Medical Center, Geert Grooteplein-Zuid 10, 6525 GA, Nijmegen, The Netherlands; Department of Medical Microbiology and Infectious Diseases, Erasmus MC, 's-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.
Jacques F
JF
Meis
Department of Internal Medicine, Radboud University Medical Center, Geert Grooteplein-Zuid 10, 6525 GA, Nijmegen, The Netherlands; Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ, Nijmegen, The Netherlands; Hasselt University, Martelarenlaan 42, BE3500, Hasselt, Belgium. Electronic address: inge.gyssens@radboudumc.nl.
Inge C
IC
Gyssens
D000038:Abscess / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods*; D001442:Bacteroides Infections / Q000175:diagnosis*; D001441:Bacteroides fragilis / Q000302:isolation & purification*; D001706:Biopsy; D017719:Diabetic Foot / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
Acute bacterial skin and skin structure infections; Bacteroides fragilis; Culture; Diagnosis; Microbiology; Real-time polymerase chain reaction