A CRISPR/Cas9 system adapted for gene editing in marine algae.
Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Marianne
M
Nymark
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Amit Kumar
AK
Sharma
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Torfinn
T
Sparstad
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Atle M
AM
Bones
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Per
P
Winge
D059001:Aquatic Organisms / Q000235:genetics*; D064113:CRISPR-Cas Systems; D017377:Diatoms / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*