Recurrent genetic defects in classical Hodgkin lymphoma cell lines.
Genetic analysis of classical Hodgkin lymphoma (cHL) has been hampered by the paucity of Hodgkin cells in biopsies and their poor growth in vitro. However, a wealth of information has been obtained from cHL cell lines. Here we report results of whole-exome sequencing and karyotypic analysis of five cHL cell lines. Four genes with potentially pathogenic single nucleotide variants (SNV) were detected in three cell lines. SNV were also detected in seventeen HL-related genes and three mitosis-related genes. Copy number variants were detected in four HL-related genes in all five cell lines. Given the high degree of aneuploidy in HL, mitosis-related genes were screened for defects. One mitotic gene (NCAPD2) was amplified in all five HL cell lines, and two genes (FAM190A, PLK4) were amplified in four cell lines. These results suggest that genomic instability of HL may be due to defects in genes involved in chromosome duplication and segregation.
a Department of Pathology, Yale School of Medicine , New Haven , CT , USA.
S David
SD
Hudnall
a Department of Pathology, Yale School of Medicine , New Haven , CT , USA.
Hailong
H
Meng
a Department of Pathology, Yale School of Medicine , New Haven , CT , USA.
Larissa
L
Lozovatsky
b Department of Genetics, Yale School of Medicine , New Haven , CT , USA.
Peining
P
Li
c Yale Cancer Center, Yale School of Medicine , New Haven , CT , USA.|d Department of Medicine (Hematology), Yale School of Medicine , New Haven , CT , USA.
Matthew
M
Strout
a Department of Pathology, Yale School of Medicine , New Haven , CT , USA.|e Interdepartmental Program in Computational Biology and Bioinformatics , Yale University , New Haven , CT , USA.|f Department of Immunobiology, Yale School of Medicine , New Haven , CT , USA.
Steven H
SH
Kleinstein
D000483:Alleles; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D019295:Computational Biology; D056915:DNA Copy Number Variations; D020869:Gene Expression Profiling; D063990:Gene Ontology; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006689:Hodgkin Disease / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D008938:Mitosis / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D000073359:Exome Sequencing
Cytogenetics; Hodgkin lymphoma; genetics; karyotype; whole-exome sequencing