Identification and Quantification Analysis on the Chemical Constituents from Traditional Mongolian Medicine Flos Scabiosae Using UHPLC-DAD-Q-TOF-MS Combined with UHPLC-QqQ-MS.
In this study, a systematic method was established for the qualitative and quantitative analysis of the major constituents in Flos Scabiosae (FS). First, Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was developed for the identification of the multi-constituents in FS. A total of 48 compounds (9 phenolic acids, 24 flavonoids, 8 iridoids and 7 others) were unambiguously or tentatively identified, including 25 compounds (flavonoids, phenolic acids) identified in FS for the first time. Second, ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometry (UHPLC-MS-MS) was developed for the quantitative analysis of 10 phenolic compounds. Ten compounds, either with high contents or strong bioactivities, were chosen as markers. This analytical method was validated through intra- and inter-day precision, repeatability and stability, with respective relative standard deviations <4.43, <8.64, <4.60 and <3.65%, respectively. The limits of detection and quantification were <1.09 and <16.96 ng/mL, respectively. The overall recoveries ranged from 96.47 to 103.94% .Then the validated method was applied to determine 10 batches of FS. The results indicated that the new method can be applied for the qualitative and quantitative analysis of FS.
Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, No. 56 Yangming Road, Nanchang 330006, PR China.
Hui
H
Ouyang
Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China.
Tianer
T
Li
Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China.
Mingzhen
M
He
Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China.
Zhifeng
Z
Li
National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, No. 56 Yangming Road, Nanchang 330006, PR China.
Ting
T
Tan
Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China.
Wugang
W
Zhang
Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China.
Yan
Y
Li
National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, No. 56 Yangming Road, Nanchang 330006, PR China fengyulin2003@126.com.
Yulin
Y
Feng
Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, No. 56 Yangming Road, Nanchang 330006, PR China.
Shilin
S
Yang
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D049917:Dipsacaceae / Q000737:chemistry*; D056785:Medicine, Mongolian Traditional; D053719:Tandem Mass Spectrometry