Resonance Rayleigh Scattering Spectra of an Ion-Association Complex of Naphthol Green B-Chitosan System and Its Application in the Highly Sensitive Determination of Chitosan.
This work describes a highly-sensitive and accurate approach for the determination of chitosan (CTS) using Naphthol Green B (NGB) as a probe in the Resonance Rayleigh scattering (RRS) method. The interaction between CTS and NGB leads to notable enhancement of RRS, and the enhancement is proportional to the concentration of CTS over a certain range. Under optimum conditions, the calibration curve of ΔI against CTS concentration was ΔI = 1860.5c + 86.125 (c, µg/mL), R² = 0.9999, and the linear range and detection limit (DL) were 0.01-5.5 µg/mL and 8.87 ng/mL. Moreover, the effect of the molecular weight of CTS on the accurate quantification of CTS was studied. The experimental data were analyzed through linear regression analysis using SPSS20.0, and the molecular weight was found to have no statistical significance. This method has been applied to assay two CTS samples and obtained good recovery and reproducibility.
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China. zhangweiai0629@126.com.
Weiai
W
Zhang
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China. mcjane2013@163.com.
Caijuan
C
Ma
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China. suzhq@scnu.edu.cn.|Key Research Center of Liver Regulation for Hyperlipidemia SATCM/Class III Laboratory of Metabolism SATCM, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China. suzhq@scnu.edu.cn.
Zhengquan
Z
Su
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China. angell_bai@163.com.
Yan
Y
Bai
D002138:Calibration; D048271:Chitosan / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D008970:Molecular Weight; D009282:Naphthalenesulfonates / Q000737:chemistry*; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*
D005290:Ferric Compounds; D009282:Naphthalenesulfonates; D048271:Chitosan; C574940:naphthol green B
Naphthol Green B; Resonance Rayleigh scattering spectra; chitosan