IL-17A promotes microglial activation and neuroinflammation in mouse models of intracerebral haemorrhage.
Microglial activation is an important contributor to neuroinflammation in intracerebral haemorrhage (ICH). IL-17A has been demonstrated to be involved in neuroinflammatory diseases such as multiple sclerosis. However, the exact mechanism of IL-17A mediated microglial activation in ICH has not been well identified. The purpose of this experiment is to investigate the role of IL-17A in ICH induced microglial activation and neuroinflammation. ICH mice were made by injection of autologous blood model. IL-17A expression and inflammatory factors in perihematomal region, and neurological function of mice were examined after ICH. In addition, IL-17A-neutralizing antibody was utilized to potentially prevent microglial activation and neuroinflammation in ICH mice. The expression of IL-17A, inflammatory factors and microglial activation in perihematomal region were significantly increased, and neurological function of mice was impaired after ICH. In addition, IL-17A Ab prevented ICH-induced cytokine expression, including TNF-α, IL-1β and IL-6, and downstream signaling molecules, including MyD88, TRIF, IκBα, and NF-κBp65 expression, and attenuated microglial activation. IL-17A Ab significantly reduced brain water content and improved neurological function of ICH mice. In conclusion, our results demonstrated that IL-17A was involved in ICH-induced microglial activation and neuroinflammation. IL-17A Ab might also provide a promising therapeutic strategy in ICH.
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Anyong
A
Yu
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Haizhen
H
Duan
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Tianxi
T
Zhang
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Yong
Y
Pan
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Zhi
Z
Kou
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Xiaojun
X
Zhang
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Yuanlan
Y
Lu
Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Song
S
Wang
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China. Electronic address: yangzhao5140@sohu.com.
Zhao
Z
Yang
D000818:Animals; D015153:Blotting, Western; D002543:Cerebral Hemorrhage / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D020381:Interleukin-17 / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000276:immunology*
D020381:Interleukin-17
ICH; IL-17A; Microglia; Neuroinflammation