Galla chinensis Compounds Remineralize Enamel Caries Lesions in a Rat Model.
To investigate the effect of Galla chinensis chemical compounds on enamel caries remineralization in rats.



A total of 40 rats were inoculated with Streptococcus sobrinus 6715 and fed a cariogenic diet (Keyes 2000). The rats were randomly divided into 4 groups and treated topically twice a day with each of the following extracts (or control) for 5 weeks: distilled and deionized water (DDW, negative control); 1,000 ppm NaF (positive control); 4,000 ppm G. chinensis crude aqueous extract (GCE), or 4,000 ppm gallic acid (GA). After the experimental period, Keyes' caries diagnosis and scoring technique was applied as a preliminary evaluation on the molar teeth. For more accurate remineralization data, the residual enamel volume of the first molar and the mineral density (MD) of the enamel were further analyzed by micro-CT.



The severity of molar enamel caries decreased in the following order of treatment groups: DDW > GA > GCE > NaF (p < 0.05). In addition, the residual first molar enamel volume and MD values increased in the order of DDW, GA, GCE and NaF treatment groups (p < 0.05). In most molars, remineralization layers were observed on the surface of lesions.



G. chinensis compounds remineralize enamel caries lesions in a rat model.
College of Stomatology, Zhengzhou University, Zhengzhou, China.
Tie-Ting
TT
Zhang
Hui-Jing
HJ
Guo
Xue-Jun
XJ
Liu
Jin-Pu
JP
Chu
Xue-Dong
XD
Zhou
D000818:Animals; D003731:Dental Caries / Q000473:pathology / Q000628:therapy*; D003743:Dental Enamel / Q000187:drug effects* / Q000473:pathology; D004037:Diet, Cariogenic; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005707:Gallic Acid / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D021621:Imaging, Three-Dimensional; D008297:Male; D008903:Minerals; D008963:Molar; D051381:Rats; D017207:Rats, Sprague-Dawley; D029045:Rhus / Q000737:chemistry*; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology; D016986:Streptococcus sobrinus / Q000187:drug effects; D014089:Tooth Remineralization / Q000379:methods*; D055114:X-Ray Microtomography
D004365:Drugs, Chinese Herbal; D008903:Minerals; D005707:Gallic Acid; D012969:Sodium Fluoride