Curcumin-Loaded PLA Nanoparticles: Formulation and Physical Evaluation.
Curcumin is a polyphenolic compound derived from Curcuma domestica (Zingiberaceae) that possesses diverse pharmacological effects including anti-inflammatory, antioxidant, antimicrobial, and anticarcinogenic activities. Although phase I clinical trials have shown curcumin as a safe drug even at high doses (12 g/day) in humans, poor bioavaibility largely limits its pharmacological activity. Nanoencapsulation in biodegradable polymers is a promising alternative to improve curcumin bioavaibility. In this study, curcumin was encapsulated in biodegradable polymer poly-(lactic acid) (PLA) nanoparticles via the emulsification-solvent evaporation method. Optimization of selected parameters of this method including the type of solvent, surfactant concentration, drug loading, sonication time, and centrifugation speed, were performed to obtain polymeric nano-carriers with optimum characteristics. Dichloromethane was used as the solvent and vitamin E polyethylene glycol succinate (TPGS) was used as the surfactant. Four minutes of sonication time and centrifugation at 10500 rpm were able to produce spherical nanoparticles with average size below 300 nm. The highest encapsulation efficiency was found on PLA nanoparticles containing 5% of curcumin at 89.42 ± 1.04%. The particle size, polydispersity index, zeta potential of 5% curcumin-PLA nanoparticles were 387.50 ± 58.60 nm, 0.289 ± 0.047, and -1.12 mV, respectively. Differential Scanning Calorimetry (DSC) and X-Ray Diffraction (XRD) studies showed partial interaction between the drug and polymer.
School of Pharmacy, Bandung Institute of Technology, Ganesha 10, 40322, Bandung, Indonesia.
Heni
H
Rachmawati
School of Pharmacy, Bandung Institute of Technology, Ganesha 10, 40322, Bandung, Indonesia.
Yulia L
YL
Yanda
School of Pharmacy, Bandung Institute of Technology, Ganesha 10, 40322, Bandung, Indonesia.
Annisa
A
Rahma
Department of Applied Chemistry and Biochemical Engineering, Graduate school of Engineering, and Green Energy Research Division, Research Institute of Green Science and Technology, Shizuoka University 3-5-1 Johoku, Hamamatsu, Shizuoka 432-8561, Japan.
Nobuyuki
N
Mase
Curcumin; Nanoparticle formulation; PLA; TPGS; poly(lactic acid)