Glycoengineered Outer Membrane Vesicles: A Novel Platform for Bacterial Vaccines.
The World Health Organization has indicated that we are entering into a post-antibiotic era in which infections that were routinely and successfully treated with antibiotics can now be lethal due to the global dissemination of multidrug resistant strains. Conjugate vaccines are an effective way to create a long-lasting immune response against bacteria. However, these vaccines present many drawbacks such as slow development, high price, and batch-to-batch inconsistencies. Alternate approaches for vaccine development are urgently needed. Here we present a new vaccine consisting of glycoengineered outer membrane vesicles (geOMVs). This platform exploits the fact that the initial steps in the biosynthesis of most bacterial glycans are similar. Therefore, it is possible to easily engineer non-pathogenic Escherichia coli lab strains to produce geOMVs displaying the glycan of the pathogen of interest. In this work we demonstrate the versatility of this platform by showing the efficacy of geOMVs as vaccines against Streptococcus pneumoniae in mice, and against Campylobacter jejuni in chicken. This cost-effective platform could be employed to generate vaccines to prevent infections caused by a wide variety of microbial agents in human and animals.
Department of Biological Sciences, University of Alberta Edmonton, Alberta T6G 2E9 Canada.
Nancy L
NL
Price
Laboratory of Immunology, Faculty of Veterinary Medicine, University of Montreal Saint-Hyacinthe, Quebec, J2S 2M2 Canada.
Guillaume
G
Goyette-Desjardins
Department of Biological Sciences, University of Alberta Edmonton, Alberta T6G 2E9 Canada.
Harald
H
Nothaft
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA.
Ezequiel
E
Valguarnera
Department of Biological Sciences, University of Alberta Edmonton, Alberta T6G 2E9 Canada.
Christine M
CM
Szymanski
Laboratory of Immunology, Faculty of Veterinary Medicine, University of Montreal Saint-Hyacinthe, Quebec, J2S 2M2 Canada.
Mariela
M
Segura
Department of Biological Sciences, University of Alberta Edmonton, Alberta T6G 2E9 Canada.|Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA.
Mario F
MF
Feldman
D000818:Animals; D016123:Campylobacter jejuni / Q000276:immunology*; D002645:Chickens; D000067128:Extracellular Vesicles / Q000276:immunology*; D051379:Mice; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D011134:Polysaccharides / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification
D022242:Pneumococcal Vaccines; D011134:Polysaccharides; D018074:Vaccines, Conjugate