Local analysis of strains and rotations for macromolecular electron microscopy maps.
Macromolecular complexes perform their physiological functions by local rearrangements of their constituents and biochemically interacting with their reaction partners. These rearrangements may involve local rotations and the induction of local strains causing different mechanical efforts and stretches at the different areas of the protein. The analysis of these local deformations may reveal important insight into the way proteins perform their tasks. In this paper we introduce a method to perform this kind of local analysis using Electron Microscopy volumes in a fully objective and automatic manner. For doing so, we exploit the continuous nature of the result of an elastic image registration using B-splines as its basis functions. We show that the results obtained by the new automatic method are consistent with previous observations on these macromolecules.
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain; Univ. San Pablo - CEU, Campus Urb. Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain. Electronic address: coss@cnb.csic.es.
C O S
CO
Sorzano
Univ. San Pablo - CEU, Campus Urb. Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain.
A
A
Martín-Ramos
Univ. San Pablo - CEU, Campus Urb. Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain.
F
F
Prieto
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
R
R
Melero
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J
J
Martín-Benito
IMPMC, Sorbonne Universités - CNRS UMR 7590, UPMC University Paris 6, MNHN, IRDUMR206, 75005 Paris, France.
S
S
Jonic
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J
J
Navas-Calvente
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J
J
Vargas
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J
J
Otón
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
V
V
Abrishami
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J M
JM
de la Rosa-Trevín
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J
J
Gómez-Blanco
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J L
JL
Vilas
Univ. Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain.
R
R
Marabini
Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain.
J M
JM
Carazo
D000255:Adenosine Triphosphate / Q000737:chemistry; D000465:Algorithms; D001331:Automation; D001426:Bacterial Proteins / Q000737:chemistry; D001696:Biomechanical Phenomena; D018834:Chaperonin 60 / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D008854:Microscopy, Electron / Q000379:methods*; D000069396:Mitochondrial Ribosomes / Q000737:chemistry; D008962:Models, Theoretical; D018832:Molecular Chaperones / Q000737:chemistry; D011485:Protein Binding; D012399:Rotation
D001426:Bacterial Proteins; D018834:Chaperonin 60; C068113:GrpE protein, Bacteria; D006360:Heat-Shock Proteins; D046911:Macromolecular Substances; D018832:Molecular Chaperones; D000255:Adenosine Triphosphate
Elastic registration; Local deformation analysis; Local rotations; Local strains; Single particle analysis