Preparation and optimization of a drug delivery system based on berberine chloride-immobilized MgAl hydrotalcite.
Hydrotalcite (HT), also known as a layered double hydroxide (LDH) compound, has been widely used in past years in the formulation of drugs due to its specific properties including good biocompatibility, null toxicity, high chemical stability and pH-dependent solubility which aid in drug controlled release. In this work, berberine chloride (BBC) class antibacterial agent was immobilized into magnesium-aluminum LDH in order to improve the drug efficiency as well as to achieve the controlled release property. BBC molecules were immobilized into MgAl LDH through a conventional ion exchange reaction and co-precipitation method. The ion-exchange experiments of BBC on MgAl LDH were investigated with particular attention paid to the influence of the layer charge, the nature of the intercalated anion and the morphology. The immobilization efficiency was dependent upon the LDH properties and the immobilization process. Characterization by powder x-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy and contact angle measurements revealed that the interaction of BBC with MgAl LDH occurs by adsorption rather than intercalation of BBC within LDH layers. In vitro anti-bacterial tests were carried out using disc diffusion assay to prove the effectiveness of these novel biohybrid beads as a controlled drug delivery method. Consequently, the BBC-LDH co-precipitated formulation revealed an enhanced anti-bacterial activity compared to the ion-exchanged formulation not only due to an improvement of chemical stability and retained amount of BBC molecules but also due to the release property.
Laboratoire de Physique des Matériaux Lamellaires et Nano-Matériaux Hybrides, Faculté des Sciences de Bizerte, Université de Carthage, 7021 Bizerte, Tunisia; Institut des Sciences Analytiques UMR CNRS 5280, Université Claude Bernard-Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France. Electronic address: mohamed.djebbi@etu.univ-lyon1.fr.
Mohamed Amine
MA
Djebbi
Laboratoire de Physique des Matériaux Lamellaires et Nano-Matériaux Hybrides, Faculté des Sciences de Bizerte, Université de Carthage, 7021 Bizerte, Tunisia.
Zaineb
Z
Bouaziz
Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques de Fès, Université Sidi Mohamed Ben Abdellah, 1213 Fès-Saïs, Morocco.
Alae
A
Elabed
Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques de Fès, Université Sidi Mohamed Ben Abdellah, 1213 Fès-Saïs, Morocco.
Moulay
M
Sadiki
Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques de Fès, Université Sidi Mohamed Ben Abdellah, 1213 Fès-Saïs, Morocco.
Soumya
S
Elabed
Institut des Sciences Analytiques UMR CNRS 5280, Université Claude Bernard-Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France; Irstea, 5 Rue de la Doua, 69100, Villeurbanne, France.
Philippe
P
Namour
Institut des Sciences Analytiques UMR CNRS 5280, Université Claude Bernard-Lyon 1, 5 Rue de la Doua, 69100 Villeurbanne, France.
Nicole
N
Jaffrezic-Renault
Laboratoire de Physique des Matériaux Lamellaires et Nano-Matériaux Hybrides, Faculté des Sciences de Bizerte, Université de Carthage, 7021 Bizerte, Tunisia.
Abdesslem Ben Haj
AB
Amara
D000536:Aluminum Hydroxide / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D001599:Berberine / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D004338:Drug Combinations; D016503:Drug Delivery Systems; D007474:Ion Exchange; D008276:Magnesium Hydroxide / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D001599:Berberine; C010467:hydrotalcite; C013591:aluminum hydroxide, magnesium hydroxide, drug combination; D000536:Aluminum Hydroxide; D008276:Magnesium Hydroxide
Aluminum chloride hexahydrate (PubChem CID: 24564); Aluminum nitrate nanohydrate (PubChem CID: 24567); Anti-bacterial activity; Berberine Chloride (PubChem CID: 12456); Berberine chloride (BBC); Drug controlled release; Immobilization; Layered double hydroxide (LDH); Magnesium chloride hexahydrate (PubChem CID: 24644); Magnesium nitrate hexahydrate (PubChem CID: 202877); Phosphate-buffered saline (PubChem CID: 24978514); Sodium hydroxide (PubChem CID: 14798); Urea (PubChem CID: 1176)