Columbianadin Inhibits Cell Proliferation by Inducing Apoptosis and Necroptosis in HCT116 Colon Cancer Cells.
Columbianadin (CBN), a natural coumarin from Angelica decursiva (Umbelliferae), is known to have various biological activities including anti-inflammatory and anti-cancer effects. In this study, the anti-proliferative mechanism of actions mediated by CBN was investigated in HCT-116 human colon cancer cells. CBN effectively suppressed the growth of colon cancer cells. Low concentration (up to 25 µM) of CBN induced apoptosis, and high concentration (50 µM) of CBN induced necroptosis. The induction of apoptosis by CBN was correlated with the modulation of caspase-9, caspase-3, Bax, Bcl-2, Bim and Bid, and the induction of necroptosis was related with RIP-3, and caspase-8. In addition, CBN induced the accumulation of ROS and imbalance in the intracellular antioxidant enzymes such as SOD-1, SOD-2, catalase and GPx-1. These findings demonstrate that CBN has the potential to be a candidate in the development of anti-cancer agent derived from natural products.
College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
Ji In
JI
Kang
College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
Ji-Young
JY
Hong
Department of Food Science and Nutrition, Pukyong National University, Busan 46241, Republic of Korea.
Jae Sue
JS
Choi
College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
Sang Kook
SK
Lee
Apoptosis; Colon cancer; Columbianadin; Necroptosis; Oxidative stress