In-Vitro Effect of Casein Phosphopeptide Amorphous Calcium Phosphate on Enamel Susceptibility to Staining by Tea during Bleaching Treatment.
OBJECTIVE
Bleached enamel is more susceptible to staining, and application of remineralizing agents may decrease enamel susceptibility to staining. This study sought to assess the effect of casein phosphopeptide amorphous calcium phosphate (CPP-ACP) on enamel susceptibility to staining during bleaching treatment.


METHODS
Forty central and lateral incisors and first premolar teeth were evaluated in four groups of 10. Group one specimens were subjected to in-office bleaching. Group two underwent in-office bleaching followed by surface treatment with CPP-ACP. Group three specimens received home bleaching and group four underwent home bleaching followed by CPP-ACP surface treatment. After each course of daily bleaching, specimens were immersed in tea solution. Home bleaching (15% carbamide peroxide) was performed for 14 days and in-office bleaching (40% hydrogen peroxide) was carried out in two sessions with an eight-day interval. The color of specimens was analyzed at baseline and post-intervention using Easy Shade Shade-Selection Device. Two-way ANOVA was used to evaluate the effects of bleaching type and surface treatment on color change. Then, the means were compared by Tukey's HSD test (P=0.05).


RESULTS
The interaction effect of surface treatment and type of bleaching was not significant on any color parameter (P>0.05). Surface treatment had significant effects on ΔL (P=0.004). Type of bleaching had a significant effect on "b" parameter (P=0.00). The effect of bleaching type on ΔE was significant (P=0.00) but the effect of surface treatment was not (P=0.34).


CONCLUSIONS
CPP-ACP had no significant effect on preventing enamel staining by tea during bleaching treatment.
Associate Professor, Babol Dental Research Center, School of Dentistry, Babol University of Medical Sciences, Babol, Iran.
Homayoom
H
Alaghemand
Assisstant Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Operative Dentistry, Faculty of Dentistry, Tehran University of Medical Sciences, International Campus, Tehran, Iran.
Sedighe Sadat
SS
Hashemi Kamangar
Assisstant Professor, Department of Operative Dentistry, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
Nafiseh
N
Zarenegad
Private Practice of Operative Dentistry, Sari, Iran.
Negin
N
Tabari
Assistant Professor, Department of Operative Dentistry, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
Hoorieh
H
Abedi
Assistant Professor, Department of Social Medicine and Health, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Soraya
S
Khafri
Casein Phosphopeptide-Amorphous Calcium Phosphate Nanocomplex; Staining; Tea; Tooth Bleaching