Photo-thermal therapy of bladder cancer with Anti-EGFR antibody conjugated gold nanoparticles.
The aim of this study was to enhance the effectiveness of photo thermal therapy (PTT) in the targeting of superficial bladder cancers using a green light laser in conjunction with gold nanoparticles (GNPs) conjugated to antibody fragments (anti-EGFR). GNPs conjugated with anti-EGFR-antibody fragments were used as probes in the targeting of tumor cells and then exposed to a green laser (532nm), resulting in the production of sufficient thermal energy to kill urothelial carcinomas both in vitro and in vivo. Nanoparticles conjugated with antibody fragments are capable of damaging cancer cells even at relatively very low energy levels, while non-conjugated nanoparticles would require an energy level of 3 times under the same conditions. The lower energy required by the nanoparticles allows this method to destroy cancerous cells while preserving normal cells when applied in vivo. Nanoparticles conjugated with antibody fragments (anti-EGFR) require less than half the energy of non-conjugated nanoparticles to kill cancer cells. In an orthotopic bladder cancer model, the group treated using PTT presented significant differences in tumor development.
Institute of BioMedical Engineering, National Cheng Kung University, Tainan, Taiwan and Department of Urology, China Medical University Beigang Hospital, Yunlin, Taiwan, jerrychen119@gmail.com.
Chieh Hsiao
CH
Chen
Institute of BioMedical Engineering, National Cheng Kung University, Tainan, Taiwan.
Yi-Jhen
YJ
Wu
Institute of BioMedical Engineering, National Cheng Kung University, Tainan, Taiwan.
Jia-Jin
JJ
Chen
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000276:immunology*; D006046:Gold; D006801:Humans; D007128:Immunoglobulin Fragments / Q000008:administration & dosage; D028022:Low-Level Light Therapy / Q000379:methods*; D053768:Metal Nanoparticles / Q000627:therapeutic use / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D058726:Nanoconjugates / Q000627:therapeutic use* / Q000648:ultrastructure; D036103:Nanotechnology; D001749:Urinary Bladder Neoplasms / Q000628:therapy* / Q000648:ultrastructure
D000906:Antibodies; D007128:Immunoglobulin Fragments; D058726:Nanoconjugates; D006046:Gold; D066246:ErbB Receptors