Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris.
We conducted a survey of fungal endophytes in 582 germinated seeds belonging to 11 Colombian cultivars of the common bean (Phaseolus vulgaris). The survey yielded 394 endophytic isolates belonging to 42 taxa, as identified by sequence analysis of the ribosomal DNA internal transcribed spacer (ITS) region. Aureobasidium pullulans was the dominant endophyte, isolated from 46.7 % of the samples. Also common were Fusarium oxysporum, Xylaria sp., and Cladosporium cladosporioides, but found in only 13.4 %, 11.7 %, and 7.6 % of seedlings, respectively. Endophytic colonization differed significantly among common bean cultivars and seedling parts, with the highest colonization occurring in the first true leaves of the seedlings.
Life Sciences Innovation Center, University of California, Davis - Chile, Andrés Bello 2299 No. 1102, Providencia, Santiago, Chile; Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia.
Soroush
S
Parsa
Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia.
Adriana M
AM
García-Lemos
Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia.
Katherine
K
Castillo
Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia.
Viviana
V
Ortiz
Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia.
Luis Augusto Becerra
LA
López-Lavalle
Statistical Consultant, 3034 Boulder Place, Davis, CA 95618, USA.
Jerome
J
Braun
Sustainable Perennial Crops Laboratory, United States Department of Agriculture, Agricultural Research Service, Building 001, BARC-W, Beltsville, MD 20705, USA. Electronic address: Fernando.Vega@ars.usda.gov.
Fernando E
FE
Vega
D044822:Biodiversity; D003105:Colombia; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D027805:Phaseolus / Q000382:microbiology*; D012639:Seeds / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
Aureobasidium pullulans; Biological control; Endophytic; Fungal biology; Seed-borne fungi