Odontoblastic inductive potential of epithelial cells derived from human deciduous dental pulp.
For the dentin regeneration, dental epithelial cells are indispensible and must possess odontoblastic induction capability. Epithelial cell-like stem cells were recently identified in human deciduous dental pulp (DPESCs). However, their cellular characteristics remain poorly defined. The purpose of this study was to characterize DPESCs compared to HAT-7 ameloblastic cells. Expression levels of ameloblast-specific markers [odontogenic ameloblast-associated protein (Odam), matrix metalloproteinase (Mmp)-20, amelogenin, and ameloblastin] were detected in DPESCs. Co-culturing odontoblastic MDPC-23 cells with DPESCs increased expression of odontoblast differentiation markers (Dmp1 and Dspp) from days 4 to 10, while the expression of bone sialoprotein rapidly decreased. MDPC-23 cells cultured in DPESC-conditioned medium (CM) showed increased Dspp promoter activity compared with control MDPC-23 cultures. Mineralization was first observed in the CM groups from day 4 and proceeded rapidly until day 14, whereas mineralized nodules were found from day 7 in control media-cultured cells. In conclusion, DPESCs in human deciduous pulp possess ameloblast-like characteristics and differentiation properties, and substances derived from DPESCs promote odontoblastic differentiation. Thus, our results indicate that DPESCs can be a realistic epithelial source for use in odontoblastic induction and dentin formation of dental mesenchymal cells.
Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 110-749, Republic of Korea.
Hye-Kyung
HK
Lee
Department of Orthodontics, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 110-749, Republic of Korea.
Ji-Won
JW
Park
Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 110-749, Republic of Korea.
You-Mi
YM
Seo
Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 110-749, Republic of Korea.
Ha Hoon
HH
Kim
Laboratory of Molecular Genetics and Stem Cell Differentiation, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 110-749, Republic of Korea.
Gene
G
Lee
Department of Dental Hygiene, Namseoul University, Cheonan, 331-707, Republic of Korea.
Hyun-Sook
HS
Bae
Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehagro, Chongro-gu, Seoul, 110-749, Republic of Korea. jcapark@snu.ac.kr.
Joo-Cheol
JC
Park
D015415:Biomarkers; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation
D015415:Biomarkers
Deciduous pulp; Dental epithelial cell; Epithelial-mesenchymal interaction; Induction; Odontoblast