Regular plateletpheresis increased basal concentrations of soluble P-selectin in healthy donors: Possible involvement of endothelial cell activation?
BACKGROUND
We explored the effects of repeated plateletpheresis on the platelet P-selectin expression and soluble P-selectin (sP-selectin) concentrations in platelet donors.


METHODS
Totally 289 platelet donors and 97 first-time whole blood (WB) donors were enrolled from the blood donor registry at the Fujian provincial blood center, China. The accumulative numbers of plateletpheresis in the last 2 y for participants were recorded, and the basal concentrations of platelet count, sP-selectin and total platelet P-selectin (pP-selectin) were determined.


RESULTS
Platelet donors had significantly higher basal concentrations of sP-selectin compared to WB donors (24.12±7.33ng/mL vs. 20.74±5.44ng/mL, P<0.0001), with no difference in platelet count and pP-selectin concentrations. Increased numbers of platelet donation were correlated with a steady increase of sP-selectin (r=0.18, P=0.002). Multivariate regression analysis identified that the frequency of plateletpheresis is an independent factor for the rise of the sP-selectin concentration (t=2.64, P=0.009) while no association was found for pP-selectin and platelet count.


CONCLUSIONS
Repeated plateletpheresis could result in an increased basal concentration of sP-selectin in blood donors whereas not an alteration in the concentrations of total platelet P-selectin. It remains to be determined whether this might be a consequence of endothelial activation or platelet activation or some other phenomenon.
Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China; Fujian Provincial Blood Center, Fuzhou 350004, China. Electronic address: fjbccy@hotmail.com.
Ying
Y
Chen
Quanzhou Blood Center, Quanzhou 362018, China.
Yisheng
Y
Lin
Fujian Provincial Blood Center, Fuzhou 350004, China.
Haijuan
H
Lin
Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China.
Jingrong
J
Xiao
Fujian Provincial Blood Center, Fuzhou 350004, China.
Li
L
Jiang
Fujian Provincial Blood Center, Fuzhou 350004, China.
Cen
C
Chen
Fujian Provincial Blood Center, Fuzhou 350004, China.
Xiaofu
X
Zhuo
Fujian Provincial Blood Center, Fuzhou 350004, China.
Wenhua
W
Huang
Fujian Provincial Blood Center, Fuzhou 350004, China.
Jia
J
Zeng
Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna 1090, Austria.
Markus
M
Dettke
D000328:Adult; D001782:Blood Donors; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D019007:P-Selectin / Q000097:blood*; D010983:Plateletpheresis; D012995:Solubility
D019007:P-Selectin
Endothelial cells activation; Healthy donors; Plateletpheresis; Platelets; Soluble P-selectin