You-Gui pills promote nerve regeneration by regulating netrin1, DCC and Rho family GTPases RhoA, Racl, Cdc42 in C57BL/6 mice with experimental autoimmune encephalomyelitis.
BACKGROUND
You-Gui pills (YGPs) are an effective traditional Chinese formula being used clinically for the treatment of multiple sclerosis (MS). Previous studies demonstrated that YGPs exerted the potent neuroprotective effects in murine models of experimental autoimmune encephalomyelitis (EAE), which is an equivalent animal model for multiple sclerosis (MS). However, the mechanism of YGPs functions remained unclear.


OBJECTIVE
The aim of this study was to evaluate the therapeutic effect of YGPs in MOG35-55-induced EAE mice and to further elucidate the underlying molecular mechanism.


METHODS
Female C57BL/6 mice were divided into six groups, including the non-treated EAE model, prednisone acetate- and 1.2, 2.4 or 4.8g/kg YGPs-treated EAE groups, and a normal control group. The EAE model was established by injecting the mice subcutaneously with MOG35-55 antigen. The body weights were measured and the neurological functions were scored in each group. The pathology and morphology of the brain and spinal cord was examined. The expression of MAP-2 was detected by immunofluorescent staining. The levels of netrin1, DCC, RhoA, Rac1, and Cdc42 were assayed by immunohistochemistry, qRT-PCR and Western blot on day 40 post-immunization (PI).


RESULTS
YGPs treatments significantly reduced neurological function scores in EAE mice, where the inflammatory infiltration was reduced and the axon and myelin damage in both brain and spinal cord was alleviated. In the brain and spinal cord tissues, YGPs increased the expression of neuronal factors MAP-2, netrin1 and DCC. The expression of Rac1 and Cdc42 were increased, while RhoA was reduced following YGPs treatments.


CONCLUSIONS
Our results demonstrated that YGPs exhibited a neuroprotective effect on promoting nerve regeneration at the brain and spinal cord in EAE mice induced by MOG35-55. Netrin1, DCC and the Rho family GTPases of RhoA, Racl, Cdc42 were involved in mediating the effects of YGPs on nerve regeneration.
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Xiaomin
X
Ji
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Haolong
H
Liu
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Chen
C
An
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Yongqiang
Y
Wang
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Hui
H
Zhao
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Qiuxia
Q
Zhang
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Ming
M
Li
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Fang
F
Qi
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China.
Zhenzhen
Z
Chen
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China. Electronic address: wxj0517@sina.com.
Xiujuan
X
Wang
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, PR China. Electronic address: tmwangl@ccmu.edu.cn.
Lei
L
Wang
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D000075389:DCC Receptor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005260:Female; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D063308:Myelin-Oligodendrocyte Glycoprotein; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects; D000075388:Netrin-1; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D010446:Peptide Fragments; D008517:Phytotherapy; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013607:Tablets; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
D000075389:DCC Receptor; C501910:Dcc protein, mouse; D004365:Drugs, Chinese Herbal; D063308:Myelin-Oligodendrocyte Glycoprotein; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D013607:Tablets; D025521:Tumor Suppressor Proteins; C445081:myelin oligodendrocyte glycoprotein (35-55); C552041:yougui; D000075388:Netrin-1; D020741:rho GTP-Binding Proteins
Betaine (PubChem CID: 247); Chlorogenic acid (PubChem CID 1794427); Cinnamaldehyde (PubChem CID: 637511); Experimental autoimmune encephalomyelitis; Fuziline (PubChem CID 14163819); Hyperin (PubChem CID: 5281643); Loganin (PubChem CID: 87691); Neoline (PubChem CID: 120682); Nerve regeneration; Netrin1/DCC; Quercitrin (PubChem CID: 5280459); Rho family GTPases RhoA, Rac1 and Cdc42; Songorine (PubChem CID 71456946); Sweroside (PubChem CID: 161036); You-Gui Pills