[Determination of selenium in whole blood by microwave digestionatomic fluorescence spectrometry].
OBJECTIVE
To establish a rapid, simple, and accurate method for measuring selenium in whole blood by atomic fluorescence spectrometry.


METHODS
The whole blood samples were treated by microwave digestion and the acids were dispelled. After that the samples were pretreated with 50% hydrochloric acid as a reducing agent. Then the content of selenium in the pretreated samples was measured by hydride generation atomic fluorescence spectrometry.


RESULTS
The calibration curve for selenium is linear in the range of 0~60 μg/L (r=0.9999). The detection limit, relative standard deviation, and recovery rate were 0.133 μg/L, 1.22%~2.08%, and 96.5%~101.8%, respectively.


CONCLUSIONS
This method is simple, with less consumption of chemical reagents, less pollution, and enough accuracy and sensitivity for determination of selenium in whole blood samples.
Department of Occupational Diseases and Toxication, Beijing Chao-Yang Hospital Affiliated to Capital University of Medical Science, Beijing 100020, China.
Jing
J
Ma
Huiling
H
Li
D002138:Calibration; D006801:Humans; D006851:Hydrochloric Acid; D007202:Indicators and Reagents; D057230:Limit of Detection; D008872:Microwaves; D012643:Selenium / Q000097:blood*; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D012643:Selenium; D006851:Hydrochloric Acid