Characterization of a polysaccharide from Rosa davurica and inhibitory activity against neutrophil migration.
The rapid recruitment of neutrophils from peripheral blood into infected sites is critical step for inflammatory responses; however, the excessive and improper recruitment can lead to serious tissue damages. Thus, it is a promising strategy to inhibit their excessive recruitment for treating inflammation-related disease. Here, we isolated a polysaccharide (RDPA1) from Rosa davurica, to evaluate its physicochemical property and inhibitory effects on neutrophil migration. RDPA1 was obtained by hot-water extraction, ethanol precipitation, and fractionated by DEAE-cellulose and Sepharose CL-6B columns. RDPA1 significantly inhibited in vitro migration of human neutrophils evaluated by transwell chamber and impacted the migratory behavior observed by time-lapsed microscopy, we found the migrated distance and average velocity of RDPA1-treated cells were greatly reduced. In addition, RDPA1 treatment impaired in vivo neutrophil infiltration in the peritonitis mice. RDPA1 exhibited significant blocking capacity of the interaction between β2 integrins and ICAM-1 evaluated by flow cytometry and in vitro protein binding assay. Together, these results suggest RDPA1 could be considered as a potential candidate for developing a novel anti-inflammatory agent.
Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China; Wood Material Science and Engineering Key Laboratory of Jilin Province, Beihua University, Jilin 132013, China.
Haibin
H
Tong
Wood Material Science and Engineering Key Laboratory of Jilin Province, Beihua University, Jilin 132013, China.
Guiquan
G
Jiang
Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China.
Xingang
X
Guan
Laboratory of Cell Imaging, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China.
Hong
H
Wu
Department of Cardiology, The General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
Kangxing
K
Song
Affiliated Hospital, Northeast Normal University, Changchun 130024, China.
Kuan
K
Cheng
Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China. Electronic address: sunxinbh@126.com.
Xin
X
Sun
D000818:Animals; D018821:CD18 Antigens / Q000378:metabolism; D002850:Chromatography, Gel; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008024:Ligands; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011485:Protein Binding; D027826:Rosa / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D018821:CD18 Antigens; D008024:Ligands; D011134:Polysaccharides; D018799:Intercellular Adhesion Molecule-1; D009240:N-Formylmethionine Leucyl-Phenylalanine
Neutrophil migration; Polysaccharide; Rosa davurica