Proteomic analysis associated with coronary artery dilatation caused by Kawasaki disease using serum exosomes.
BACKGROUND
The aim of this study was to investigate the serum exosome proteome profile of coronary artery dilatation (CAD) caused by Kawasaki disease (KD).


METHODS
Two-dimensional electrophoresis was implemented on proteins of serum exosomes obtained from children with CAD caused by KD and from healthy controls. Differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry analysis.


RESULTS
We identified 38 differentially expressed proteins (13 up-regulated and 25 down-regulated) from serum exosomes of patients with CAD caused by KD compared with healthy controls. Expression levels of three differentially expressed proteins (leucine-rich alpha-2-glycoprotein, sex hormone-binding globulin, and serotransferrin) were validated using western blot analysis. Classification and protein-protein network analysis showed that they are associated with multiple functional groups involved in the acute inflammatory response, defense response, complement activation, humoral immune response, and response to wounding. The majority of the proteins are involved in the inflammation and coagulation cascades.


CONCLUSIONS
These findings establish a comprehensive proteome profile of CAD caused by KD and increase our knowledge of scientific insight into its mechanisms.
Guangzhou Women and Children's Medical Center, Guangzhou 510120, Guangdong, China.
Li
L
Zhang
Department of Obstetrics and Gynecology, the First Affiliated Hospital, Sun Yat-sen University,Guangzhou, Guangdong 510700, China.
Wei
W
Wang
Department of Pediatrics, Foshan Maternal and Children Hospital, Foshan, 528000, Guangdong, China.
Jun
J
Bai
Guangzhou Women and Children's Medical Center, Guangzhou 510120, Guangdong, China.
Yu-Fen
YF
Xu
Guangzhou Youdi Biotechnology Company, Guangzhou 510663, Guangdong, China.
Lai-Qing
LQ
Li
Guangzhou Women and Children's Medical Center, Guangzhou 510120, Guangdong, China.
Liang
L
Hua
Guangzhou Women and Children's Medical Center, Guangzhou 510120, Guangdong, China.
Li
L
Deng
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China.
Hong-Ling
HL
Jia
D003324:Coronary Artery Disease / Q000209:etiology* / Q000235:genetics; D003331:Coronary Vessels; D004106:Dilatation; D015180:Electrophoresis, Gel, Two-Dimensional; D055354:Exosomes; D006801:Humans; D009080:Mucocutaneous Lymph Node Syndrome / Q000150:complications / Q000235:genetics*; D020543:Proteome; D040901:Proteomics
D020543:Proteome
Coronary artery dilatation; Dilatação da artéria coronária; Doença de Kawasaki; Exosome; Exossoma; Kawasaki disease; Proteomics; Proteómica