WDR26 is a new partner of Axin1 in the canonical Wnt signaling pathway.
The stability of β-catenin is very important for canonical Wnt signaling. A protein complex including Axin/APC/GSK3β phosphorylates β-catenin to be degraded by ubiquitination with β-TrCP. In the recent study, we isolated WDR26, a protein that binds to Axin. Here, we found that WDR26 is a negative regulator of the canonical Wnt signaling pathway, and that WDR26 affected β-catenin levels. In addition, WDR26/Axin binding is involved in the ubiquitination of β-catenin. These results suggest that WDR26 plays a negative role in β-catenin degradation in the Wnt signaling pathway.
Department of Molecular Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Japan.
Toshiyasu
T
Goto
Department of Molecular Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Japan.
Junhei
J
Matsuzawa
Molecular Profiling Research Center for Drug Discovery, National Institutes of Advanced Industrial Science and Technology, Tokyo, Japan.
Shun-Ichiro
S
Iemura
Molecular Profiling Research Center for Drug Discovery, National Institutes of Advanced Industrial Science and Technology, Tokyo, Japan.
Tohru
T
Natsume
Department of Molecular Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Japan.
Hiroshi
H
Shibuya
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D060466:Axin Protein / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D054875:Ubiquitination; D060449:Wnt Signaling Pathway; D014981:Xenopus; D051176:beta Catenin / Q000378:metabolism
C555451:AXIN1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D060466:Axin Protein; D011506:Proteins; C543172:WDR26 protein, human; D051176:beta Catenin
Axin1; WDR26; Wnt; ubiquitination; β-catenin