Identification of miRNomes reveals ssc-miR-30d-R_1 as a potential therapeutic target for PRRS viral infection.
Porcine reproductive and respiratory syndrome virus (PRRSV) is known to cause reproductive disorders, such as abortion, in pregnant sows as well as immunosuppressive respiratory complications, leading to severe respiratory tract infections in young pigs. In this study, an in-depth analysis of the miRNomes in mock- and virus-infected pig lungs was carried out. We found that highly expressed ssc-miR-30d-R_1 was decreased in infected lungs, and reduced levels were significantly correlated with infection by PRRSV. Moreover, ssc-miR-30d-R_1 was shown to target Toll-like receptor 4 (TLR4) and to suppress the production of immune cytokines through inhibition of the TLR4/MyD88/NF-κB pathway. ssc-miR-30d-R_1 significantly reduced viral infections and pathological changes in pig lungs in vivo. Our current study reveals the miRNomes of PRRSV-infected pig lungs and indicates that ssc-miR-30d-R_1 is potential therapeutic agent for controlling PRRSV infection.
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Chengmin
C
Wang
Beijing Institute of Transfusion Medicine, Academy of Military Medicine Sciences, Beijing, 100850, P.R China.
Yanyu
Y
Zhang
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Jing
J
Luo
Department of Infectious Disease, Hangzhou Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, 310021, P.R China.
Hua
H
Ding
Department of Infectious Diseases, Zhejiang Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, 310051, P.R China.
Shelan
S
Liu
Department of Zoology, Faculty of Science, Kafr El sheikh University, Kafr El sheikh 33516, Egypt.
Said
S
Amer
Department of Infectious Disease, Hangzhou Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, 310021, P.R China.
Li
L
Xie
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Wenting
W
Lyv
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Wen
W
Su
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Meng
M
Li
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Qinmiao
Q
Sun
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Jiayin
J
Dai
Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R China.
Hongxuan
H
He
D000818:Animals; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008168:Lung / Q000473:pathology* / Q000821:virology*; D035683:MicroRNAs / Q000032:analysis*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000473:pathology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000254:growth & development*; D013552:Swine
D035683:MicroRNAs