Slowed relaxation of diaphragm in septic rats is associated with reduced expression of sarco-endoplasmic reticulum CA2+-ATPase genes SERCA1 and SERCA2.
The aim of this study was to study the effects of sepsis on diaphragm relaxation properties and the associated expression of sarco-endoplasmic reticulum Ca2+-ATPase genes SERCA1 and SERCA2.



Rats were randomized to undergo either sham surgery or cecal ligation and puncture (CLP). Diaphragm isometric relaxation parameters were measured after 24 h. The mRNA expression and protein content of SERCA1 and SERCA2 in diaphragm muscles were determined.



Both diaphragm maximal twitch and tetanus relaxation rates were reduced. Twitch half-relaxation time was prolonged after normalization to half of peak twitch tension. The mRNA expression and protein content of SERCA1 and SERCA2 were decreased.



Slowed relaxation of the diaphragm in septic rats was associated with reduced expression of SERCA1 and SERCA2. Muscle Nerve 54: 1108-1113, 2016.
Department of Anesthesiology, First People's Hospital, School of Medicine, Shanghai Jiaotong University, 100 Hai Ning Road, Shanghai, 20080, China.
Jin
J
Wu
Department of Anesthesiology, First People's Hospital, School of Medicine, Shanghai Jiaotong University, 100 Hai Ning Road, Shanghai, 20080, China.
Jian You
JY
Zhang
Department of Anesthesiology, First People's Hospital, School of Medicine, Shanghai Jiaotong University, 100 Hai Ning Road, Shanghai, 20080, China.
Yuan
Y
Gong
Department of Anesthesiology, First People's Hospital, School of Medicine, Shanghai Jiaotong University, 100 Hai Ning Road, Shanghai, 20080, China.
Shi Tong
ST
Li
D000818:Animals; D002431:Cecostomy / Q000009:adverse effects; D002432:Cecum / Q000601:surgery; D003964:Diaphragm / Q000378:metabolism* / Q000503:physiopathology*; D004195:Disease Models, Animal; D004558:Electric Stimulation; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D066298:In Vitro Techniques; D008026:Ligation / Q000009:adverse effects; D009119:Muscle Contraction / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D018805:Sepsis / Q000209:etiology / Q000473:pathology*
D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
SERCA; diaphragm; muscle relaxation; muscle weakness; ryanodine receptor; sepsis