Sulfurovum riftiae sp. nov., a mesophilic, thiosulfate-oxidizing, nitrate-reducing chemolithoautotrophic epsilonproteobacterium isolated from the tube of the deep-sea hydrothermal vent polychaete Riftia pachyptila.
An anaerobic, nitrate-reducing, sulfur- and thiosulfate-oxidizing bacterium, designated strain 1812ET, was isolated from the vent polychaete Riftia pachyptila, which was collected from a deep-sea hydrothermal vent on the East Pacific Rise. Cells were Gram-stain-negative rods, measuring approximately 1.05±0.11 µm by 0.40±0.05 µm. Strain 1812ETgrew at 25 - -45 °C (optimum 35 °C), with 1.5-4.0 % (w/v) NaCl (optimum 3.0 %) and at pH 5.0-8.0 (optimum pH 6.0). The generation time under optimal conditions was 3 h. Strain 1812ETwas an anaerobic chemolithotroph that grew with either sulfur or thiosulfate as the energy source and carbon dioxide as the sole carbon source. Nitrate was used as a sole terminal electron acceptor. The predominant fatty acids were C16 : 1ω7c, C18 : 1ω7c and C16 : 0. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was menaquinone MK-6 and the G+C content of the genomic DNA was 47.4 mol%. Phylogenetic analysis of the 16S rRNA gene of strain 1812ETshowed that the isolate belonged to the Epsilonproteobacteria, and its closest relatives were Sulfurovum lithotrophicum 42BKTTand Sulfurovum aggregans Monchim 33T(98.3 and 95.7 % sequence similarity, respectively). DNA-DNA relatedness between strain 1812ETand the type strain of S. lithotrophicum was 29.7 %, demonstrating that the two strains are not members of the same species. Based on the phylogenetic, molecular, chemotaxonomic and physiological evidence, strain 1812ETrepresents a novel species within the genus Sulfurovum, for which the name Sulfurovum riftiae sp. nov. is proposed. The type strain is 1812ET(=DSM 101780T=JCM 30810T).
Institute of Earth Ocean and Atmospheric Science, Rutgers University, New Brunswick, NJ, USA.|Institute of Marine and Coastal Science, ISMAR, National Research Council of Italy, CNR Ancona, Italy.
Donato
D
Giovannelli
Institute of Earth Ocean and Atmospheric Science, Rutgers University, New Brunswick, NJ, USA.|Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ, USA.
Matthew
M
Chung
Institute of Earth Ocean and Atmospheric Science, Rutgers University, New Brunswick, NJ, USA.|Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ, USA.
Justin
J
Staley
Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA.
Valentin
V
Starovoytov
Sorbonne Universités, UPMC Univ. Paris 6, CNRS, Laboratoire d'Ecogéochimie des Environnements Benthiques, Observatoire Océanologique, Banyuls-sur-Mer, France.
Nadine
N
Le Bris
Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ, USA.|Institute of Earth Ocean and Atmospheric Science, Rutgers University, New Brunswick, NJ, USA.
Costantino
C
Vetriani
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020565:Epsilonproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D060086:Hydrothermal Vents / Q000382:microbiology*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011077:Polychaeta / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013885:Thiosulfates / Q000378:metabolism; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D009566:Nitrates; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D013885:Thiosulfates; D024482:Vitamin K 2; C042455:menaquinone 6
East Pacific Rise; Sulfurovum; Epsilonproteobacteria; deep-sea hydrothermal vents; nitrate reduction; sulfur oxidation