High-frequency electron paramagnetic resonance of metal-containing porphyrin compounds using a microcantilever.
In this article, we report a novel technique of high-frequency electron paramagnetic resonance (HFEPR) using a microcantilever. In this method, a sample is mounted on a cantilever, and the field-gradient force associated with EPR absorption is detected as a cantilever bending. By using a micrometer-sized cantilever, this technique can be applied to a very tiny sample on the order of μg. In addition, the use of a piezoresistive cantilever makes the experimental setup easy and compact. In this study, we applied this technique to multi-frequency HFEPR measurements of metal-containing porphyrin compounds, which are an important composing element of metal-containing proteins and coenzymes such as hemoglobin and cyanocobalamin.
Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
Eiji
E
Ohmichi
Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
Tsubasa
T
Okamoto
Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
Masaaki
M
Mitani
Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
Hideyuki
H
Takahashi
Molecular Photoscience Research Center, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan. Electronic address: hohta@kobe-u.ac.jp.
Hitoshi
H
Ohta
D003300:Copper / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods; D006427:Hemin / Q000737:chemistry*; D060526:Magnetic Fields; D008665:Metalloporphyrins / Q000737:chemistry*
D008665:Metalloporphyrins; C010560:copper tetraphenylporphyrin; D006427:Hemin; D003300:Copper
Cantilever; Electron paramagnetic resonance (EPR); Metal-containing porphyrin