Fabrication of polyhydroxybutyrate (PHB)/γ-Fe2O3 nanocomposite film and its properties study.
Magnetic separation has numerous advantages in isolating cancerous and normal cells used in the diagnosis and treatment sectors. Here, we produced magnetic nanocomposite films made of polyhydroxybutyrate (PHB)/magnetite nanoparticles (γ-Fe2O3), and the properties of the films by SEM, TEM, FTIR, DMTA, contact angle, and cellular analyses were investigated. The microscopic images showed uniform distribution of γ-Fe2O3 magnetic nanoparticles in polymeric matrix. The chemical bounds between magnetic nanoparticles and polymeric matrix demonstrated using the FTIR spectrophotometer. The DMTA and contact angle results indicated an increase in the glass transition temperature and hydrophilic properties of nanocomposites is achieved by increasing the magnetic nanoparticles amount in polymer matrix. The cellular results were showed that adhesion of cancer cells compared to normal cells was significantly enhanced by the induction of a magnetic field. These nanocomposite films can be used as a substrate for cellular adhesion and separation processes.
a Biomaterials Department , Science and Research Branch, Islamic Azad University , Tehran , Iran.
Shokooh
S
Moghaddam
b Biomaterial Department , Iran Polymer and Petrochemical Institute , Tehran , Iran.
Mohammad
M
Taghi Khorasani
c Faculty of Biomedical Engineering, Department of Tissue Engineering , AmirKabir University of Technology , Tehran , Iran.
Hesam
H
Hosseinkazemi
d Faculty of Biomedical Engineering , Tonekabon Branch, Islamic Azad University , Tonekabon , Iran.
Esmaeil
E
Biazar
e Department of Materials Science and Engineering , Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil.
Mahyar
M
Fazeli
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology; D011091:Polyesters / Q000737:chemistry*; D000091402:Prohibitins
D001672:Biocompatible Materials; D005290:Ferric Compounds; C000717328:PHB protein, human; D011091:Polyesters; D000091402:Prohibitins; C107955:poly(4-hydroxybutanoate); C000499:ferric oxide
Magnetic nanocomposite; cell separation; physical and mechanical analyses; polyhydroxybuthyrate (PHB); γ-Fe2O3 nanoparticles