A new two-dimensional chromatographic method for separation of saponins from steamed Panax notoginseng.
The root and rhizome of Panax notoginseng (PNG) are used as folk medicine. Recent studies have reported PNG to possess immunomodulatory, cardioprotective, hepatoprotective, anti-diabetic and anticancer activities among a host of other pharmacological effects. The main active constituents responsible for these pharmacological effects are saponins. It has also been proven that the chemical constituents of steamed PNG differs from the raw form. Traditional methods of separating individual components in crude extracts are usually tedious, almost irreproducible and time-consuming. In this study, an automated multi-step preparative separation system, known as Sepbox afforded a quick, reproducible and fast separation of saponins from PNG. With Sepbox, a total of 11 saponins of high purity were obtained in a short period of time. The separated compounds were identified as notoginsenosides R1, T5, ginsenosides Rb1, Rg1, Rg2, Rh1, Rh4, Rd, 20 (S) -Rg3 and a mixture of ginsenosides Rk1 and Rg5.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjia Lane, Nanjing 210009, China.
Jimmy K
JK
Lelu
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjia Lane, Nanjing 210009, China.
Qi
Q
Liu
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjia Lane, Nanjing 210009, China.
Raphael N
RN
Alolga
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjia Lane, Nanjing 210009, China.
Yong
Y
Fan
Kunming Institute of Botany, CAS, China. Electronic address: xlw@mail.kib.ac.cn.
Wei-Lie
WL
Xiao
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjia Lane, Nanjing 210009, China. Electronic address: Qilw@cpu.edu.cn.
Lian-Wen
LW
Qi
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjia Lane, Nanjing 210009, China. Electronic address: liping2004@126.com.
Ping
P
Li
D002853:Chromatography, Liquid / Q000379:methods; D053723:Panax notoginseng / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification*
D012503:Saponins
Panax notoginseng; Rare ginsenosides; Saponins; Sepbox