Modulatory Effect of Taurine on 7,12-Dimethylbenz(a)Anthracene-Induced Alterations in Detoxification Enzyme System, Membrane Bound Enzymes, Glycoprotein Profile and Proliferative Cell Nuclear Antigen in Rat Breast Tissue.
The modulatory effect of taurine on 7,12-dimethylbenz(a)anthracene (DMBA)-induced breast cancer in rats was studied. DMBA (25 mg/kg body weight) was administered to induce breast cancer in rats. Protein carbonyl levels, activities of membrane bound enzymes (Na(+) /K(+) ATPase, Ca(2+) ATPase, and Mg(2+) ATPase), phase I drug metabolizing enzymes (cytochrome P450, cytochrome b5, NADPH cytochrome c reductase), phase II drug metabolizing enzymes (glutathione-S-transferase and UDP-glucuronyl transferase), glycoprotein levels, and proliferative cell nuclear antigen (PCNA) were studied. DMBA-induced breast tumor bearing rats showed abnormal alterations in the levels of protein carbonyls, activities of membrane bound enzymes, drug metabolizing enzymes, glycoprotein levels, and PCNA protein expression levels. Taurine treatment (100 mg/kg body weight) appreciably counteracted all the above changes induced by DMBA. Histological examination of breast tissue further supported our biochemical findings. The results of the present study clearly demonstrated the chemotherapeutic effect of taurine in DMBA-induced breast cancer.
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India. vanithakalappan@gmail.com.
Manickam Kalappan
MK
Vanitha
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Kuppusamy
K
Baskaran
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Kuppusamy
K
Periyasamy
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Sundaramoorthy
S
Selvaraj
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Aruldoss
A
Ilakkia
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Dhiravidamani
D
Saravanan
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Ramachandran
R
Venkateswari
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Balasundaram
B
Revathi Mani
Department of Biomedical Sciences, College of Health Sciences, Arsi University, Asella, Ethiopia.
Pandi
P
Anandakumar
Department of Medical Biochemistry, Dr. ALMPGIBMS, University of Madras, Taramani Campus, Chennai, 600 113, India.
Dhanapal
D
Sakthisekaran
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity; D003580:Cytochromes / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D050216:Metabolic Detoxication, Phase I / Q000235:genetics; D050217:Metabolic Detoxication, Phase II / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics* / Q000378:metabolism; D050050:Protein Carbonylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013654:Taurine / Q000494:pharmacology*
D000970:Antineoplastic Agents; D002273:Carcinogens; D003580:Cytochromes; D006023:Glycoproteins; D026901:Membrane Transport Proteins; D018809:Proliferating Cell Nuclear Antigen; D013654:Taurine; D015127:9,10-Dimethyl-1,2-benzanthracene
Breast Cancer; DMBA; Drug Metabolizing Enzymes; Glycoproteins; Membrane Bound Enzymes; Proliferative Cell Nuclear Antigen; Taurine