Study on DNA damaging effects of 4-nonylphenol using erythrocytes from peripheral circulation, gill and kidney of fish Channapunctatus.
The present study aimed to evaluate the genotoxic effect of 4-nonylphenol (NP) on blood cells of fish Channapunctatus. Fish were exposed to three sublethal concentrations (0.15 mg l⁻¹; 0.31 mg l⁻¹ and 0.63 mg l⁻¹) of 4-NP for 24, 48, 72 and 96 hrs. Blood cells from kidney, gills and peripheral circulation were analyzed for the presence of micronuclei and other changes in the erythrocytes. Significant changes were observed in all the experimental groups tested when compared with control. Highest genotoxicity was observed in blood cells obtained from gills (MN-2.92%, aberrant cell- 70.64%), followed by kidney (MN-1.34%, aberrant cells-64.94%), were least effect was observed in blood cells obtained from peripheral circulation (MN-0.88%, aberrant cells-46.27%).Therefore, micronucleus test performed on blood cells obtained from different sources showed that gills were more sensitive as compared to peripheral blood and kidney revealing genotoxic effect of 4-NP on fish C. punctatus.
Madhu
M
Sharma
Pooja
P
Chadha
D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects*; D005399:Fishes / Q000097:blood*; D005880:Gills / Q000098:blood supply*; D007668:Kidney / Q000098:blood supply*; D015162:Micronucleus Tests; D010636:Phenols / Q000633:toxicity*
D010636:Phenols; C041594:4-nonylphenol