[LRRC3B is Downregulated in Non-small Cell Lung Cancer and Connected with Cell Proliferation and Invasion].
BACKGROUND
LRRC3B expression is downregulated in many kinds of malignant tumors and it is regarded as tumor inhibition protein. However, the expression pattern and biological effect are still unclear in non-small cell lung cancer (NSCLC). Study of human cancer microarray showed LRRC3B was downregulated in breast cancer and colorectal cancer, which declares that LRRC3B takes part in tumor formation. This study is to investigate the expression of LRRC3B in lung cancer cell lines and the influence of LRRC3B during cell proliferation, invasion and cell cycle.


METHODS
The expression of LRRC3B was detected through Western blot and Realtime RT-PCR. MTT assay, colony formation assay and matrigel invasion assay were performed to explore the effect of LRRC3B on cell proliferation, invasion and cell cycle. LRRC3B siRAN was transfected in lung cancer cell line H3255, and then the effect of LRRC3B on cell proliferation, invasion and cell cycle was analyzed.


RESULTS
During 4 of 9 lung cancer cell lines, the expression of LRRC3B is downregulated compared with normal epithelial cells. Cell proliferation and invasion were inhibited in A549 and H460 after LRRC3B transfected. LRRC3B suppressed the progress of cell cycle and downregulated the expression of cyclin D1 and MMP9. The activity of proliferation and invasion was promoted after LRRC3B was knocked out in H3255.


CONCLUSIONS
LRRC3B expression downreguated in lung cancer cell lines and LRRC3B could inhibit lung cancer cell proliferation, invasion and cell cycle progress. LRRC3B could be a new target for lung cancer therapy. .
Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Liang
L
Kan
Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Meng
M
Zhang
Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Ping
P
He
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism
C530104:CCND1 protein, human; C477813:LRRC3B protein, human; D009363:Neoplasm Proteins; D019938:Cyclin D1; D020780:Matrix Metalloproteinase 9