Imported dengue from 2013 Angola outbreak: Not just serotype 1 was detected.
All the reports from Angola's 2013 dengue outbreak revealed serotype 1. However, previously dengue serotypes 1-4 have been reported in Africa and in 2014 serotype 4 was reported in Angola.



To report dengue serotypes in patients returning from Angola during 2013 outbreak.



Retrospective, cross-sectional study. We serotyped the dengue by an in house Polymerase Chain Reaction technique in randomly selected cases.



From the 2013 Angola's dengue outbreak we treated 47 adult patients. None had history of past dengue. A combo kit test for dengue revealed positive NS1 antigen in 39 and IgM antibodies in 8. From 17 randomly patients tested by RNA Real Time-PCR, 11 were positive: 7 for DENV-1, 2 for DENV-2, 1 for DENV-3 (co-infected with DENV-1) and 1 for DENV-4. None had a complicated or fatal evolution.



Unlike previous reports the 4 serotypes were detected, and this resulted in a different epidemiological situation, raising the risk of future outbreaks of severe dengue.
Department of Infectious Diseases, Centro Hospitalar S. João, Porto, Portugal; Instituto de Inovação e Investigação em Saúde (I3S), Grupo de I&D em Nefrologia e Doenças Infecciosas, Instituto Nacional de Engenharia Biomédica (INEB), Portugal. Electronic address: candida.abreu@gmail.com.
Cândida
C
Abreu
Department of Infectious Diseases, Centro Hospitalar S. João, Porto, Portugal.
André
A
Silva-Pinto
Clinical Pathology Service, Centro Hospitalar S. João, Portugal.
Daniela
D
Lazzara
Clinical Pathology Service, Centro Hospitalar S. João, Portugal.
Joana
J
Sobrinho-Simões
Clinical Pathology Service, Centro Hospitalar S. João, Portugal; EPIUnit, Institute of Public Health, University of Porto, Portugal.
João Tiago
JT
Guimarães
Department of Infectious Diseases, Centro Hospitalar S. João, Porto, Portugal; Instituto de Inovação e Investigação em Saúde (I3S), Grupo de I&D em Nefrologia e Doenças Infecciosas, Instituto Nacional de Engenharia Biomédica (INEB), Portugal.
António
A
Sarmento
D000328:Adult; D000810:Angola / Q000453:epidemiology; D003430:Cross-Sectional Studies; D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005260:Female; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011174:Portugal; D012189:Retrospective Studies; D065288:Serogroup; D014195:Travel
Angola; Angola’s dengue outbreak; Dengue; Dengue serotype