Skin optical clearing potential of disaccharides.
Skin optical clearing can significantly enhance the ability of biomedical optical imaging. Some alcohols and sugars have been selected to be optical clearing agents (OCAs). In this work, we paid attention to the optical clearing potential of disaccharides. Sucrose and maltose were chosen as typical disaccharides to compare with fructose, an excellent monosaccharide-OCA, by using molecular dynamics simulation and an ex vivo experiment. The experimental results indicated that the optical clearing efficacy of skin increases linearly with the concentration for each OCA. Both the theoretical predication and experimental results revealed that the two disaccharides exerted a better optical clearing potential than fructose at the same concentration, and sucrose is optimal. Since maltose has an extremely low saturation concentration, the other two OCAs with saturation concentrations were treated topically on rat skin in vivo, and optical coherence tomography imaging was applied to monitor the optical clearing process. The results demonstrated that sucrose could cause a more significant increase in imaging depth and signal intensity than fructose.
Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, Ministry of Education.
Wei
W
Feng
Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, Ministry of Education.
Rui
R
Shi
Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, Ministry of Education.
Ning
N
Ma
Saratov State University, Research-Educational Institute of Optics and Biophotonics, 83 Astrakhanskaya Street, Saratov 410012, Russia.
Daria K
DK
Tuchina
Saratov State University, Research-Educational Institute of Optics and Biophotonics, 83 Astrakhanskaya Street, Saratov 410012, RussiaeInstitute of Precise Mechanics and Control RAS, 24 Rabochaya Street, Saratov 410028, RussiafTomsk State University, Labor.
Valery V
VV
Tuchin
Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, 1037 Luoyu Road, Wuhan, Hubei 430074, ChinabHuazhong University of Science and Technology, Ministry of Education.
Dan
D
Zhu
D000818:Animals; D004187:Disaccharides / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D056004:Molecular Dynamics Simulation; D051381:Rats; D012542:Scattering, Radiation; D012867:Skin / Q000000981:diagnostic imaging*; D041623:Tomography, Optical Coherence / Q000379:methods*
D004187:Disaccharides