Immunoassay for the Detection of Animal Central Nervous Tissue in Processed Meat and Feed Products.
An indirect competitive enzyme-linked immunosorbent assay (icELISA) based on the detection of the thermal-stable central nervous tissue (CNT) marker protein, myelin basic protein (MBP), was developed to detect animal CNT in processed meat and feedstuffs. Two meat samples (cooked at 100 °C for 30 min and autoclaved at 133 °C for 20 min) of bovine brain in beef and two feed samples (bovine brain meal in beef meal and in soybean meal) were prepared at levels of 0.0008, 0.0031, 0.0063, 0.0125, 0.025, 0.05, 0.1, 0.2, 0.4, 0.8, and 1.6%. An anti-MBP monoclonal antibody (mAb3E3) was produced using the hybridoma technique and characterized using Western blot. The optimized icELISA was CNT-specific without cross-reactivity with either meat (beef and pork) or soybean meal samples and had low intra-assay (%CV ≤ 3.5) and interassay variability (%CV ≤ 3.3), with low detection limits for bovine MBP (6.4 ppb) and bovine CNT spiked in both meat (0.05%) and feed (0.0125%) samples. This assay is therefore suitable for the quantitative detection of trace amounts of contaminated animal CNT in processed food and feed products.
Department of Nutrition, Food and Exercise Sciences, Florida State University , Tallahassee, Florida 32306-1493, United States.
Qinchun
Q
Rao
Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University , Manhattan, Kansas 66506, United States.
Juergen A
JA
Richt
Department of Nutrition, Food and Exercise Sciences, Florida State University , Tallahassee, Florida 32306-1493, United States.
Yun-Hwa Peggy
YH
Hsieh
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002490:Central Nervous System / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D004676:Myelin Basic Protein / Q000032:analysis*; D009417:Nerve Tissue / Q000737:chemistry; D013552:Swine
D004676:Myelin Basic Protein
ELISA; bovine spongiform encephalopathy; central nervous tissue; immunoassay; myelin basic protein; specified risk material