The Primary Structure of β(I)-Chain of Hemoglobin from Snake Sindhi Krait (Bungarus sindanus sindanus).
The amino acid sequence of β(I)-globin chain from Sindhi Krait (Bungarus sindanus sindanus) was determined to study the molecular evolution among snakes. The hemoglobin was isolated from the red blood cells and was analyzed by ion-exchange chromatography (IEX). The crude globin was subjected to reversed phased-high performance liquid chromatography (RP-HPLC) using C4 column. The N-terminal sequences of intact globin chains and tryptic peptides were determined by Edman degradation in a pulsed liquid gas phase sequencer using an online Phenylthiohydantoin analyzer. Sindhi Krait is expected to express three hemoglobin components that are composed of β(II), β(I), α(D) and α(A)-globin chains, as apparent by IEX, RP-HPLC and N-terminal sequence analyses. Sequence alignment and phylogenetic analyses of β(I) globin chain from Sindhi Krait showed closest relationship with β(I) globin chain from Rattlesnake, Water snake and Indigo snake. Interestingly, comparison of primary sequence of β(I) globin chain of Sindhi Krait with human β chain revealed 63 % similarity along with the retention of all heme contact points. Variations among the two sequences were prominent at αβ contact points and in regions directly not important for function.
H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
Humera
H
Waheed
Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, 02881, USA.
Hilary
H
Friedman
National Center for Proteomics, University of Karachi, Karachi, 75270, Pakistan.
Syed Faraz
SF
Moin
National Center for Proteomics, University of Karachi, Karachi, 75270, Pakistan.
Shamshad
S
Zarina
Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, 02881, USA. aahmed@chapman.edu.|Chapman University School of Pharmacy, 9401 Jeronimo Road, Irvine, CA, 92618, USA. aahmed@chapman.edu.
Aftab
A
Ahmed
D000595:Amino Acid Sequence; D000818:Animals; D017838:Bungarus / Q000235:genetics*; D006454:Hemoglobins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D010802:Phylogeny; D030162:Reptilian Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D055544:beta-Globins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D006454:Hemoglobins; D030162:Reptilian Proteins; D055544:beta-Globins
Bungarus sindanus sindanus; Hemoglobin; Primary structure; Reptiles; Sindhi Krait; Structure-function