Microfluidics: The future of microdissection TESE?
Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.
a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA.|b Department of Mechanical Engineering , University of Utah , Salt Lake City , Utah , USA.
Raheel
R
Samuel
a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA.
Odgerel
O
Badamjav
c Huntsman Cancer Institute, University of Utah , Salt Lake City , Utah , USA.
Kristin E
KE
Murphy
d Division of Urology, Department of Surgery , University of Utah , Salt Lake City , Utah , USA.
Darshan P
DP
Patel
e Department of Electrical & Computer Engineering , University of Utah , Salt Lake City , Utah , USA.
Jiyoung
J
Son
b Department of Mechanical Engineering , University of Utah , Salt Lake City , Utah , USA.
Bruce K
BK
Gale
a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA.|d Division of Urology, Department of Surgery , University of Utah , Salt Lake City , Utah , USA.
Douglas T
DT
Carrell
a Andrology and IVF Laboratories, University of Utah , Salt Lake City , Utah , USA.|d Division of Urology, Department of Surgery , University of Utah , Salt Lake City , Utah , USA.
James M
JM
Hotaling
D002985:Clinical Protocols; D006801:Humans; D008297:Male; D042282:Microdissection; D044085:Microfluidics; D013059:Spectrum Analysis, Raman; D053625:Sperm Retrieval
IVF; microTESE; microfluidics; sperm separation