Fundamental monomeric biomaterial diagnostics by radio frequency signal analysis.
We present a new diagnostic technique of fundamental monomeric biomaterials that do not rely on any enzyme or chemical reaction. Instead, it only uses radio frequency (RF) signal analysis. The detection and classification of basic biomaterials, such as glucose and albumin, were demonstrated. The device was designed to generate a strong resonance response with glucose solution and fabricated by simple photolithography with PDMS (Polydimethylsiloxane) well. It even was used to detect the level of glucose in mixtures of glucose and albumin and in human serum, and it operated properly and identified the glucose concentration precisely. It has a detection limit about 100μM (1.8mg/dl), and a sensitivity about 58MHz per 1mM of glucose and exhibited a good linearity in human blood glucose level. In addition, the intrinsic electrical properties of biomaterials can be investigated by a de-embedding technique and an equivalent circuit analysis. The capacitance of glucose containing samples exhibited bell-shaped Gaussian dispersion spectra around 2.4GHz. The Albumin solution did not represent a clear dispersion spectra compared to glucose, and the magnitude of resistance and inductance of albumin was higher than that of other samples. Other parameters also represented distinguishable patterns to classify those biomaterials. It leads us to expect future usage of our technique as a pattern-recognizing biosensor.
School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea; Nano-Bioresearch Center, Korea Institute of Science and Technology, 39-1 Hawolgok Dong, Songbuk Gu, Seoul 136-791, Republic of Korea.
Jae-Hoon
JH
Ji
Nano-Bioresearch Center, Korea Institute of Science and Technology, 39-1 Hawolgok Dong, Songbuk Gu, Seoul 136-791, Republic of Korea.
Kyeong-Sik
KS
Shin
School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
Shinill
S
Kang
Nano-Bioresearch Center, Korea Institute of Science and Technology, 39-1 Hawolgok Dong, Songbuk Gu, Seoul 136-791, Republic of Korea.
Soo Hyun
SH
Lee
Nano-Bioresearch Center, Korea Institute of Science and Technology, 39-1 Hawolgok Dong, Songbuk Gu, Seoul 136-791, Republic of Korea.
Ji Yoon
JY
Kang
Department of Laboratory Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
Sinyoung
S
Kim
School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea. Electronic address: scj@yonsei.ac.kr.
Seong Chan
SC
Jun
D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D017097:Electric Impedance; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D011846:Radio Waves; D012709:Serum Albumin / Q000032:analysis*
D001786:Blood Glucose; D012709:Serum Albumin
Bio-detection; Biomaterial diagnostics; Biosensor; Dielectric relaxation; Label-free; Radio-frequency