Cross-fostering reduces obesity induced by early exposure to monosodium glutamate in male rats.
Maternal obesity programmes a range of metabolic disturbances for the offspring later in life. Moreover, environmental changes during the suckling period can influence offspring development. Because both periods significantly affect long-term metabolism, we aimed to study whether cross-fostering during the lactation period was sufficient to rescue a programmed obese phenotype in offspring induced by maternal obesity following monosodium L-glutamate (MSG) treatment. Obesity was induced in female Wistar rats by administering subcutaneous MSG (4 mg/g body weight) for the first 5 days of postnatal life. Control and obese female rats were mated in adulthood. The resultant pups were divided into control second generation (F2) (CTLF2), MSG-treated second generation (F2) (MSGF2), which suckled from their CTL and MSG biological dams, respectively, or CTLF2-CR, control offspring suckled by MSG dams and MSGF2-CR, MSG offspring suckled by CTL dams. At 120 days of age, fat tissue accumulation, lipid profile, hypothalamic leptin signalling, glucose tolerance, glucose-induced, and adrenergic inhibition of insulin secretion in isolated pancreatic islets were analysed. Maternal MSG-induced obesity led to an obese phenotype in male offspring, characterized by hyperinsulinaemia, hyperglycaemia, hyperleptinaemia, dyslipidaemia, and impaired leptin signalling, suggesting central leptin resistance, glucose intolerance, impaired glucose-stimulated, and adrenergic inhibition of insulin secretion. Cross-fostering normalized body weight, food intake, leptin signalling, lipid profiles, and insulinaemia, but not glucose homeostasis or insulin secretion from isolated pancreatic islets. Our findings suggest that alterations during the lactation period can mitigate the development of obesity and prevent the programming of adult diseases.
Department of Biotechnology, Cell Biology and Genetics, State University of Maringá/UEM, Block H67, room 19, Colombo Avenue 5790, Maringá, PR, Brazil. roapmiranda@yahoo.com.br.|Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. roapmiranda@yahoo.com.br.
Rosiane Aparecida
RA
Miranda
Department of Biotechnology, Cell Biology and Genetics, State University of Maringá/UEM, Block H67, room 19, Colombo Avenue 5790, Maringá, PR, Brazil.
Claudinéia Conationi
CC
da Silva Franco
Health Sciences Institute, Federal University of Mato Grosso, Sinop, MT, Brazil.
Júlio Cezar
JC
de Oliveira
Molecular Signaling Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Luiz Felipe
LF
Barella
Department of Biotechnology, Cell Biology and Genetics, State University of Maringá/UEM, Block H67, room 19, Colombo Avenue 5790, Maringá, PR, Brazil.
Laize Peron
LP
Tófolo
Department of Biotechnology, Cell Biology and Genetics, State University of Maringá/UEM, Block H67, room 19, Colombo Avenue 5790, Maringá, PR, Brazil.
Tatiane Aparecida
TA
Ribeiro
Department of Biotechnology, Cell Biology and Genetics, State University of Maringá/UEM, Block H67, room 19, Colombo Avenue 5790, Maringá, PR, Brazil.
Audrei
A
Pavanello
Department of Physiological Sciences Roberto Alcântara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Ellen Paula Santos
EP
da Conceição
Department of Physiological Sciences, State University of Maringá, Maringá, PR, Brazil.
Rosana
R
Torrezan
School of Medicine (Optometr), Deakin University, Waurn Ponds, Geelong, VIC, 3216, Australia.
James
J
Armitage
Department of Physiological Sciences Roberto Alcântara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Patrícia Cristina
PC
Lisboa
Department of Physiological Sciences Roberto Alcântara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Egberto Gaspar
EG
de Moura
Department of Biotechnology, Cell Biology and Genetics, State University of Maringá/UEM, Block H67, room 19, Colombo Avenue 5790, Maringá, PR, Brazil.
Paulo Cezar
PC
de Freitas Mathias
Department of Biotechnology, Cell Biology and Genetics, State University of Maringá/UEM, Block H67, room 19, Colombo Avenue 5790, Maringá, PR, Brazil.
Elaine
E
Vieira
D050154:Adiposity; D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D005260:Female; D047109:Fetal Development; D005421:Flavoring Agents / Q000008:administration & dosage / Q000009:adverse effects; D005503:Food Additives / Q000008:administration & dosage / Q000009:adverse effects; D007279:Injections, Subcutaneous; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D007774:Lactation; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D017208:Rats, Wistar; D012970:Sodium Glutamate / Q000008:administration & dosage / Q000009:adverse effects; D046509:Tissue Culture Techniques; D015430:Weight Gain
D005421:Flavoring Agents; D005503:Food Additives; D007328:Insulin; D012970:Sodium Glutamate
Cross-fostering; Monosodium glutamate; Obesity