Fas/Fas Ligand pathways gene polymorphisms in pediatric renal allograft rejection.
An essential milestone in pediatric transplantation is to find noninvasive biomarkers to monitor acute rejection (AR). In this retrospective (Case-control) study, we examined the role of Fas -670A/G and Fas Ligand (FasL) -843C/T gene polymorphisms in allograft nephropathy in pediatric renal transplant recipients.



In 47 pediatric kidney transplant recipients and 20 healthy controls, Fas -670A/G and FasL -843C/T gene polymorphisms as well as serum soluble Fas Ligand level (sFasL) were measured.



Serum sFasL levels were significantly higher in transplant recipients children than that in controls (548.25±298.64pg/ml vs 143.17±44.55pg/ml, p=0.0001). There was no significant difference between patients with AR and those without AR in regards to serum sFasL levels (567.70±279.87pg/ml vs 507.85±342.80pg/ml, p=0.56). Fas -670A/G genotypes or alleles were not significantly different between controls and transplant recipients and among transplant recipients with and without AR. (P>0.05 for all). FasL -843C/T genotypes were not different between transplant recipients and controls and among transplant recipients with and without AR (P>0.05 for all). However, Frequency of C allele in transplant patients was significantly higher than that in the control group (44.68% vs 25%, P=0.03). FasL -843C/T alleles were significantly different between patients with and without AR (P=0.03). The percentages of C allele were higher in children with AR (58.82% vs 36.67%). We found that serum FasL and serum creatinine were variables that were independently associated with AR.



This study suggests that FasL gene polymorphisms in peripheral blood might be accurate in detecting cellular AR.
Pediatric Department, Faculty of Medicine, Cairo University, Cairo, Egypt. Electronic address: fatina_fadel100@yahoo.com.
Fatina I
FI
Fadel
Pediatric Department, National Research Centre, Cairo, Egypt. Electronic address: manal_elshmaa@hotmail.com.
Manal F
MF
Elshamaa
Pediatric Department, Faculty of Medicine, Cairo University, Cairo, Egypt. Electronic address: ahmedsalah78@live.com.
Ahmed
A
Salah
Pediatric Department, Faculty of Medicine, Cairo University, Cairo, Egypt. Electronic address: marwa.nabhan@hotmail.com.
Marwa
M
Nabhan
Clinical & Chemical Pathology Department, National Research Centre, Cairo, Egypt. Electronic address: maha.rasheed@yahoo.com.
Maha
M
Rasheed
Clinical & Chemical Pathology Department, National Research Centre, Cairo, Egypt. Electronic address: solaf.kamel@yahoo.com.
Solaf
S
Kamel
Clinical & Chemical Pathology Department, National Research Centre, Cairo, Egypt. Electronic address: dina.kandil@hotmail.com.
Dina
D
Kandil
Clinical & Chemical Pathology Department, National Research Centre, Cairo, Egypt.
Eman H
EH
Thabet
D000208:Acute Disease; D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics / Q000628:therapy*; D016030:Kidney Transplantation; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D019014:fas Receptor / Q000235:genetics*
C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor
Cellular rejection; Fas Ligand; Genetic polymorphism; Pediatric transplantation