CdSe magic-sized quantum dots incorporated in biomembrane models at the air-water interface composed of components of tumorigenic and non-tumorigenic cells.
Cadmium selenide (CdSe) magic-sized quantum dots (MSQDs) are semiconductor nanocrystals with stable luminescence that are feasible for biomedical applications, especially for in vivo and in vitro imaging of tumor cells. In this work, we investigated the specific interaction of CdSe MSQDs with tumorigenic and non-tumorigenic cells using Langmuir monolayers and Langmuir-Blodgett (LB) films of lipids as membrane models for diagnosis of cancerous cells. Surface pressure-area isotherms and polarization modulation reflection-absorption spectroscopy (PM-IRRAS) showed an intrinsic interaction between the quantum dots, inserted in the aqueous subphase, and Langmuir monolayers constituted either of selected lipids or of tumorigenic and non-tumorigenic cell extracts. The films were transferred to solid supports to obtain microscopic images, providing information on their morphology. Similarity between films with different compositions representing cell membranes, with or without the quantum dots, was evaluated by atomic force microscopy (AFM) and confocal microscopy. This study demonstrates that the affinity of quantum dots for models representing cancer cells permits the use of these systems as devices for cancer diagnosis.
Institute of Environmental, Chemical and Pharmaceutical Sciences (ICAQF), Federal University of São Paulo (UNIFESP), 09913-030 Diadema, SP, Brazil.
Thiago E
TE
Goto
Institute of Environmental, Chemical and Pharmaceutical Sciences (ICAQF), Federal University of São Paulo (UNIFESP), 09913-030 Diadema, SP, Brazil; Department of Biochemistry, Federal University of São Paulo (UNIFESP), 04039-032 São Paulo, SP, Brazil.
Carla C
CC
Lopes
Department of Biochemistry, Federal University of São Paulo (UNIFESP), 04039-032 São Paulo, SP, Brazil.
Helena B
HB
Nader
Laboratory of New Insulator and Semiconductor Materials (LNMIS), Institute of Physics, Federal University of Uberlândia (UFU), 38400-902 Uberlândia, MG, Brazil.
Anielle C A
AC
Silva
Laboratory of New Insulator and Semiconductor Materials (LNMIS), Institute of Physics, Federal University of Uberlândia (UFU), 38400-902 Uberlândia, MG, Brazil.
Noelio O
NO
Dantas
Institute of Exact Sciences, Natural and Education, Federal University of TriânguloMineiro (UFTM), 38064-200 Uberaba, MG, Brazil. Electronic address: jr.siqueira@fisica.uftm.edu.br.
José R
JR
Siqueira
Institute of Environmental, Chemical and Pharmaceutical Sciences (ICAQF), Federal University of São Paulo (UNIFESP), 09913-030 Diadema, SP, Brazil. Electronic address: lcaseli@unifesp.br.
Luciano
L
Caseli
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D000388:Air / Q000032:analysis; D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry*; D002457:Cell Extracts / Q000737:chemistry*; D002460:Cell Line; D002461:Cell Line, Transformed; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D018625:Microscopy, Atomic Force; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D010718:Phosphatidylserines / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D011817:Rabbits; D018036:Selenium Compounds / Q000737:chemistry*; D013499:Surface Properties; D019076:Transgenes; D053835:Unilamellar Liposomes / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D019187:Cadmium Compounds; D002457:Cell Extracts; D015513:Oncogene Proteins; D010718:Phosphatidylserines; D018036:Selenium Compounds; D053835:Unilamellar Liposomes; C077183:human oncogene protein EJ-ras; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; C038694:dipalmitoylphosphatidylserine; C081581:colfosceril palmitate; C058667:cadmium selenide
Cancer diagnosis; CdSe magic-sized quantum dots; Langmuir–Blodgett ultrathin films; Tumorigenic and non-tumorigenic cells