Interlaboratory ring trial to evaluate CFT proficiency of European laboratories for diagnosis of glanders in equids.
To evaluate the routine complement fixation test (CFT) used to detect Burkholderia mallei antibodies in equine sera, an interlaboratory proficiency test was held with 24 European laboratories, including 22 National Reference Laboratories for glanders. The panels sent to participants were composed of sera with or without B mallei antibodies. This study confirmed the reliability of CFT and highlighted its intralaboratory reproducibility. However, the sensitivity of glanders serodiagnosis and laboratory proficiency may be improved by standardising critical reagents, including antigens, and by developing a standard B mallei serum.
European Union Reference Laboratory for Equine Diseases, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
K
K
Laroucau
European Union Reference Laboratory for Equine Diseases, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
C
C
Colaneri
Bacterial Zoonosis Unit, Paris-Est University, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
M
M
Jaÿ
Bacterial Zoonosis Unit, Paris-Est University, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
Y
Y
Corde
Bacterial Zoonosis Unit, Paris-Est University, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
A
A
Drapeau
Epidemiology Unit, Paris-Est University, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
B
B
Durand
European Union Reference Laboratory for Equine Diseases, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
S
S
Zientara
European Union Reference Laboratory for Equine Diseases, ANSES, Laboratory for Animal Health, Maisons-Alfort, France.
C
C
Beck
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D042726:Burkholderia mallei / Q000276:immunology / Q000302:isolation & purification*; D003168:Complement Fixation Tests / Q000662:veterinary*; D005060:Europe; D005260:Female; D005896:Glanders / Q000175:diagnosis*; D006736:Horses; D007753:Laboratories / Q000706:statistics & numerical data*; D015203:Reproducibility of Results
D000907:Antibodies, Bacterial
Burkholderia mallei; Complement fixation test; Glanders; Ring trial; Serology