Colorimetric and ratiometric fluorescent detection of bisulfite by a new HBT-hemicyanine hybrid.
A novel HBT-hemicyanine hybrid was prepared. This hybrid not only displays a large red-shifted (Δλ = 125 nm) emission compared to the well known ESIPT dye HBT, but also can be used as a new probe for rapid, colorimetric and ratiometric fluorescent detection of bisulfite with high selectivity and sensitivity in aqueous solution. The detection limit of this probe for HSO3(-) was calculated to be about 56 nM with a linear range of 0-25 μM. The potential application of this probe was exampled by detection of bisulfite in real food samples and living cells. Overall, this work not only provided a new ratiometric sensing platform, but also provided a new promising colorimetric and ratiometric fluorescent probe for bisulfite.
School of Chemical and Environmental Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan 430023, People's Republic of China.
Haiyan
H
Zhang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, People's Republic of China.
Zijun
Z
Huang
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, People's Republic of China. Electronic address: gf256@mail.ccnu.edu.cn.
Guoqiang
G
Feng
D052160:Benzothiazoles / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D019422:Dietary Sucrose / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D010636:Phenols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013447:Sulfites / Q000032:analysis*; D014920:Wine / Q000032:analysis
D052160:Benzothiazoles; D002232:Carbocyanines; D004396:Coloring Agents; D019422:Dietary Sucrose; D005456:Fluorescent Dyes; D010636:Phenols; D013447:Sulfites; C000601156:tetramethylene hemicyanine; C070846:2-(2-hydroxyphenyl)benzothiazole; C042345:hydrogen sulfite
Bisulfite; Colorimetric; Fluorescent; HBT-hemicyanine hybrid; Ratiometric