[Characterization of β-1, 4-mannanase from Bacillus pumilus and heterologous expression in Lactobacillus casei].
OBJECTIVE
Lactobacillus casei is widely used in food production and feed industry. The aim of this study was to construct the recombinant expression mannanase Lb. casei.


METHODS
The mature peptide gene of β-1,4-mannanase from Bacillus pumilus was cloned into expression vectors pELX1 and pELSH, then electroporated into Lb. casei, establishing an intracellular and a secretion expression mannanase Lb. casei respectively.


RESULTS
After incubation, the specific activity of β-1,4-mannanase was 23 U/mg whole cell protein for intracellular expression and 8.8 U/mL for secretion expression in supernatant.


CONCLUSIONS
Mannanase gene expression in Lb. casei provides application prospect and deserves further study.
Yexia
Y
Zou
Jinzhong
J
Lin
Xuemei
X
Bu
Lulu
L
Jiang
Zhengjun
Z
Chen
Xiangyang
X
Ge
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D015870:Gene Expression; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000235:genetics* / Q000378:metabolism; D044902:beta-Mannosidase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D044902:beta-Mannosidase