Genotype and enterotoxigenicity of Staphylococcus epidermidis isolate from ready to eat meat products.
We have previously shown that potentially pathogenic isolates of Staphylococcus epidermidis occur at high incidence in ready-to-eat food. Now, within 164 samples of ready-to-eat meat products we identified 32 S. epidermidis isolates. In 8 isolates we detected the genes encoding for staphylococcal enterotoxins, but in 7 S. epidermidis isolates these genes were not stable over passages. One isolate designated 4S was shown to stably harbour sec and sel genes. In the genome sequence of S. epidermidis 4S we identified 21,426-bp region flanked by direct-repeats, encompassing sec and sel genes, corresponding to the previously described composite staphylococcal pathogenicity island (SePI) in S. epidermidis FRI909. Alignment of S. epidermidis 4S and S. epidermidis FRI909 SePIs revealed 6 nucleotide mismatches located in 5 of the total of 29 ORFs. Genomic location of S. epidermidis 4S SePI was the same as in FRI909. S. epidermidis 4S is a single locus variant of ST561, being genetically different from FRI909. SECepi was secreted by S. epidermidis 4S to BHI broth ranging from 14 to almost 36μg/mL, to milk ranging from 6 to 9ng/mL, to beef meat juice from 2 to 3μg/mL and to pork meat juice from 1 to 2μg/mL after 24 and 48h of cultivation, respectively. We provide the first evidence that S. epidermidis occurring in food bears an element encoding an orthologue to Staphylococcus aureus SEC, and that SECepi can be produced in microbial broth, milk and meat juices. Regarding that only enterotoxins produced by S. aureus are officially tracked in food in EU, the ability to produce enterotoxin by S. epidermidis pose real risk for food safety.
Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: magdalena.podkowik@up.wroc.pl.
Magdalena
M
Podkowik
Department of Basic Sciences, Mississippi State University, Mississippi, USA.
Keun Seok
KS
Seo
Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland.
Justyna
J
Schubert
Department of Microbiology and Immunology, University of Mississippi Medical Center, USA.
Isaiah
I
Tolo
Department of Microbiology and Immunology, University of Mississippi Medical Center, USA.
D Ashley
DA
Robinson
Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland.
Jacek
J
Bania
Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland.
Jarosław
J
Bystroń
D000818:Animals; D004768:Enterotoxins / Q000235:genetics*; D005838:Genotype; D008461:Meat Products / Q000382:microbiology*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*
D004768:Enterotoxins
Food safety; SEC; Staphylococcal enterotoxin; Staphylococcal pathogenicity island; Staphylococcus epidermidis