Kordiaulvae sp. nov., a bacterium isolated from the surface of green marine algae Ulva sp.
A novel bacterial strain, SC2T, was isolated from Ulva sp., a green marine algae, which was collected from Dadeng island, Xiamen, China. Strain SC2Twas Gram-stain-negative, aerobic, rod-shaped and had no flagellum. Oxidase and catalase were positive. Strain SC2Twas able to degrade skimmed milk, agar, soluble starch, Tween 20 and Tween 80 but not colloidal chitin. Growth was observed at salinities of 1-6 % and temperatures of 15-37 °C, with optimal salinity and temperature of 2 % and 30 °C, respectively. Phylogenetic analysis based on the 16S rRNA gene indicated that strain SC2Twas affiliated to the genus Kordia, with highest sequence similarity to Kordia algicida OT-1T(97.23 %), Kordia antarctica IMCC 3317T(97.23 %) and Kordia jejudonensis SSK3-3T(97.02 %); the type strains of other species of the genus Kordia shared 93.98-95.78 % gene sequence similarity with strain SC2T. The average nucleotide identity value and estimated DNA-DNA hybridization value between strain SC2Tand the above three type strains (K. algicida OT-1T, K. antarctica IMCC 3317Tand K. jejudonensis SSK3-3T) were found to be 79.4-82.4 % and 24.2-27.0 %, respectively. The predominant fatty acids (>5.0 %) were C16 : 0, iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c), summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c) and summed feature 9 (comprising iso-C17 : 1ω9c/C16 : 0 10-methyl). The respiratory quinone was determined to be solely menaquinone-6 (MK-6). The polar lipid profile of strain SC2Tconsisted of four unknown lipids, three unidentified phospholipids, one unidentified aminolipid and phosphatidylethanolamine. The G+C content of the genomic DNA was 34.5 mol%. The combined genotypic and phenotypic data showed that strain SC2Trepresents a novel species within the genus Kordia, for which the name Kordia ulvae sp. nov. is proposed. The type strain is SC2T(= KCTC 42872T=MCCC 1A01772T=LMG 29123T).
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation, Xiamen, PR China.|School of Marine Sciences, Ningbo University, Ningbo, PR China.|Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
Feng
F
Qi
Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation, Xiamen, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|School of Marine Sciences, Ningbo University, Ningbo, PR China.
Zhaobin
Z
Huang
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation, Xiamen, PR China.|State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.|Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
Qiliang
Q
Lai
School of Marine Sciences, Ningbo University, Ningbo, PR China.
Dengfeng
D
Li
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.|Third Institute of Oceanography, SOA, Xiamen 361005, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation, Xiamen, PR China.
Zongze
Z
Shao
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D044445:Ulva / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6
Kordia ulvae; Ulva sp.