Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX.
Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows arterial sampling on repeated occasions in the same animal.



Eleven male Wistar rats were two times catheterized via a superficial branch of a femoral artery and scanned with [(11)C]MPDX and blood sampling. PET images were co-registered to a magnetic resonance imaging (MRI) template. Regional tracer distribution volumes (V T) in the brain were calculated by the Logan analysis. The procedure was repeated after 1 week.



Surgery was successful in 90 % of the cases, and discomfort was minor. The V T data showed small differences between test and retest, low between subject variability, and a strong agreement between and within subjects.



Repeated quantitative imaging with a high reproducibility is possible with this approach.
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
Jürgen W A
JW
Sijbesma
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
Xiaoyun
X
Zhou
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
David
D
Vállez García
Experimental Anesthesiology and Clinical Pharmacology Research Group, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
Martin C
MC
Houwertjes
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
Janine
J
Doorduin
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
Chantal
C
Kwizera
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
Bram
B
Maas
Groningen Institute for Evolutionary Life Sciences, University of Groningen, Nijenborgh 7, 9747AG, Groningen, The Netherlands.
Peter
P
Meerlo
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
Rudi A
RA
Dierckx
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.|Department of Biomedical Photonic Imaging, University of Twente, P.O. Box 217, 7500AE, Enschede, The Netherlands.
Riemer H J A
RH
Slart
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands.
Philip H
PH
Elsinga
University Medical Center Groningen, Department of Nuclear Medicine and Molecular Imaging, University of Groningen, Hanzeplein 1, 9713 GZ, Groningen, The Netherlands. a.van.waarde@umcg.nl.
Aren
A
van Waarde
D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods*; D001835:Body Weight; D008024:Ligands; D008297:Male; D049268:Positron-Emission Tomography / Q000379:methods*; D017208:Rats, Wistar; D043682:Receptor, Adenosine A1 / Q000378:metabolism; D015203:Reproducibility of Results; D014970:Xanthines / Q000737:chemistry*
C451639:1-methyl-8-dicyclopropylmethyl-1-methyl-3-propylxanthine; D008024:Ligands; D043682:Receptor, Adenosine A1; D014970:Xanthines
Adenosine A1 receptor; Arterial blood sampling; Brain; Positron emission tomography; Test-retest reproducibility