Carbonic Anhydrase VI Gene Polymorphism rs2274327 Relationship Between Salivary Parameters and Dental-Oral Health Status in Children.
The aim of this study was to research carbonic anhydrase (CA) VI one single-nucleotide polymorphism (SNP) and its potential association with dental-oral health status (dental caries, Plaque Index (PI) and Gingival Index (GI)) and salivary parameters (salivary buffering capacity, salivary flow rate (SFR)) in children. A total of 178 children were divided into two groups: non-carious (n = 70, 34 boys and 36 girls) and carious (n = 108, 47 boys and 61 girls). The clinical evaluations were performed according to the decayed, missing, and filled teeth (dmft/DMFT) index by a specialist. Clinical parameters including PI, GI, and simplified oral hygiene index (OHI-S) were recorded. Salivary pH (SpH) was measured using pH paper. Blood samples and unstimulated whole saliva were collected, and SFR was calculated. The CA VI rs2274327 polymorphism was determined by a LightSNiP assay on the realtime PCR system. The frequencies of rs2274327 were not significant between groups (p > 0.05). There was a positive correlation between OHI-S and SpH in the carious and non-carious groups (p < 0.05). There was no correlation among the SNPs' frequencies and OHI-S, PI, GI, SFR, and SpH (p > 0.05). CA VI SNP (rs2274327) had no statistically significant association with OHI-S, PI, GI, SFR, and SpH in the children.
Department of Pediatric Dentistry, Faculty of Dentistry, Ataturk University, Erzurum, Turkey.
Fatih
F
Sengul
Department of Pediatric Dentistry, Faculty of Dentistry, Ataturk University, Erzurum, Turkey. mnvvr_25@hotmail.com.
Munevver
M
Kilic
Department of Pediatric Dentistry, Faculty of Dentistry, Ataturk University, Erzurum, Turkey.
Taskin
T
Gurbuz
Department of Medical Genetics, Medical Faculty, Ataturk University, Erzurum, Turkey.
Sener
S
Tasdemir
D000293:Adolescent; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism; D002648:Child; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009909:Oral Health / Q000208:ethnology; D009910:Oral Hygiene; D020641:Polymorphism, Single Nucleotide; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D014421:Turkey / Q000208:ethnology
D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI
Caries; Dental health surveys; Gustin; Human; Salivary flow