Vaccine adjuvant ginsenoside Rg1 enhances immune responses against hepatitis B surface antigen in mice.
The adjuvant effect of ginsenoside Rg1 on immune responses against hepatitis B surface antigen (HBsAg) in mice was investigated. Female BALB/c mice were subcutaneously injected with saline or HBsAg antigen with or without Rg1 on days 7 and 21. Samples were collected 2 weeks after the boosting for the detection of anti-HBsAg immunoglobulin G (IgG) isotypes in sera and gamma interferon (IFN-γ) and interleukin-4 (IL-4) produced in splenocytes. The innate and adaptive immune responses were measured in mice immunized as described above. The results showed that ginsenoside Rg1 had adjuvant properties in stimulating IgG, splenocyte proliferation, and mRNA expression of cytokines IFN-γ and IL-4, as well as the expression of cell surface marker TLR4 in the HBsAg-immunized mice. These results indicate that Rg1 enhances both Th1 (IgG2b and IFN-γ) and Th2 (IgG1 and IL-4) responses. In addition, the TLR4 signaling pathway is involved in the adjuvant activities of ginsenoside Rg1.
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.|b Renhe Hospital, The Second College of Clinical Medical Science, Three Gorges University, Yichang, Hubei 443001, China.
Ding
D
Yuan
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Qin
Q
Yuan
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Qianqian
Q
Cui
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Chaoqi
C
Liu
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Zhiyong
Z
Zhou
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Haixia
H
Zhao
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Yaoyan
Y
Dun
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Ting
T
Wang
a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
Changcheng
C
Zhang
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D005260:Female; D036145:Ginsenosides / Q000008:administration & dosage*; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage*; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000276:Adjuvants, Immunologic; D036145:Ginsenosides; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; C035054:ginsenoside Rg1
adjuvant immunitaire; antigène de surface du virus de l’hépatite B (HBsAg); cell immunity; ginsenoside Rg1; ginsénoside Rg1; hepatitis B surface antigen (HBsAg); humoral immunity; immune adjuvant; immunité cellulaire; immunité humorale