Influences of limited resuscitation with plasma or plasma protein solutions on hemostasis and survival of rabbits with noncompressible hemorrhage.
Plasma infusion with or without red blood cells is the current military standard of care for prehospital resuscitation of combat casualties. We examined possible advantages of early and limited resuscitation with fresh plasma compared with a single plasma protein or crystalloid solutions in an uncontrolled hemorrhage model in rabbits.



Anesthetized spontaneously breathing rabbits (3.3 ± 0.1 kg) were instrumented and subjected to a splenic uncontrolled hemorrhage. Rabbits in shock were resuscitated at 15 minutes with Plasma-Lyte (PAL; 30 mL/kg), PAL + fibrinogen (PAL + F; 30 mL + 100 mg/kg), fresh rabbit plasma (15 mL/kg), or 25% albumin (ALB; 5 mL/kg) solution, all given in two bolus intravenous injections (15 minutes apart) to achieve a mean arterial pressure of 65 mm Hg, n = 8 to 9/group. Animals were monitored for 2 hours or until death, and blood loss was measured. Blood samples and tissues were collected and analyzed.



There were no differences among groups in baseline measures and their initial bleeding volume at 15 minutes. At 60 minutes after injury, mean arterial pressure was higher with ALB than with crystalloids (PAL or PAL + F), but shock indices were not different despite the large differences in resuscitation volumes. Fibrinogen addition to PAL only increased clot strength. Plasma resuscitation increased survival rate (75%) without significant improvement in coagulation measures. Albumin administration replenished total plasma protein and increased survival rate to 100% (p < .05 vs. crystalloids). No histological adverse events were identified in the vital organs.



Fibrinogen administration added to a compatible crystalloid did not improve hemostatic outcomes. Plasma resuscitation increased survival rate; however, its effects did not differ from those obtained with 25% ALB at one-third of the volume. The ALB advantage was consistent with our previous findings in which 5% ALB was used at a volume equal to plasma. The benefit of plasma for resuscitation may be mostly due to its ALB content rather than its coagulation proteins.
From the US Army Institute of Surgical Research, JBSA, Ft. Sam Houston, Texas.
Bijan Shams
BS
Kheirabadi
Nahir
N
Miranda
Irasema B
IB
Terrazas
Amber N
AN
Voelker
Rose C
RC
Grimm
Michael A
MA
Dubick
D000818:Animals; D000077324:Crystalloid Solutions; D004195:Disease Models, Animal; D005942:Gluconates / Q000494:pharmacology; D006470:Hemorrhage / Q000401:mortality / Q000628:therapy*; D006490:Hemostatics / Q000494:pharmacology*; D007552:Isotonic Solutions / Q000494:pharmacology; D015636:Magnesium Chloride / Q000494:pharmacology; D008297:Male; D010949:Plasma; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits; D012151:Resuscitation / Q000379:methods*; D019346:Sodium Acetate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D015996:Survival Rate
D000077324:Crystalloid Solutions; D005942:Gluconates; D006490:Hemostatics; D007552:Isotonic Solutions; C012499:Plasma-lyte 148; D015636:Magnesium Chloride; D012965:Sodium Chloride; D019346:Sodium Acetate; D011189:Potassium Chloride