Antimicrobial activity of biosilver nanoparticles produced by a novel Streptacidiphilus durhamensis strain.
OBJECTIVE
In this study, an acidophilic actinobacteria strain was used as a novel reducing agent for a single-step synthesis of nanostructure silver particles. We used a Streptacidiphilus durhamensis HGG16n isolate for efficient synthesis of bioactive silver nanoparticles [bio(AgNPs)] in an inexpensive, eco-friendly, and nontoxic manner. The obtained bio(AgNPs) exhibited unique physicochemical and biochemical properties.


METHODS
Structural, morphological, and optical properties of the synthesized biocolloids were characterized by spectroscopy, dynamic light scattering, and electron microscopy approaches. The antimicrobial activity was evaluated using the well- and disc-diffusion methods.


RESULTS
The obtained crystalline structure and stable biosynthesized silver nanoparticles ranged in size from 8 nm to 48 nm and were mostly spherical in shape. Antimicrobial assays of the silver nanoparticles against pathogenic bacteria showed the highest antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Proteus mirabilis, followed by Escherichia coli, Klebsiella pneumoniae, and Bacillus subtilis. Moreover, the synergistic effect of bio(AgNPs) with various commercially available antibiotics was also evaluated.


CONCLUSIONS
These results provide insight into the development of new antimicrobial agents along with synergistic enhancement of the antibacterial mechanism against clinical bacteria.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland. Electronic address: bbusz@chem.uni.torun.pl.
Bogusław
B
Buszewski
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
Viorica
V
Railean-Plugaru
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
Paweł
P
Pomastowski
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
Katarzyna
K
Rafińska
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
Malgorzata
M
Szultka-Mlynska
Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
Patrycja
P
Golinska
Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
Magdalena
M
Wypij
Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
Dariusz
D
Laskowski
Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
Hanna
H
Dahm
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D007711:Klebsiella pneumoniae / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D011513:Proteus mirabilis / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000378:metabolism* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013306:Streptomycetaceae / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D012834:Silver
acidophilic actinomycetes; antibacterial activity; biocolloids; green synthesis; silver nanoparticles