Nanoparticle-mediated interplay of chitosan and melatonin for improved wound epithelialisation.
Herein, we propose an innovative approach to improving wound healing. Our strategy is to deliver melatonin locally at the wound site by means of lecithin/chitosan nanoparticles. We used four types of chitosan that differed in terms of molecular weight and/or deacetylation degree. Melatonin encapsulation efficiency, nanoparticle size, zeta potential, biocompatibility and in vitro drug release were studied as a function of the type of chitosan used in preparation. The nanoparticles were evaluated in terms of their potential to promote wound epithelialisation via an in vitro scratch assay using a human keratinocyte (HaCaT) monolayer. The model wounds were treated with nanoparticle suspensions at a chitosan concentration of 5μgml(-1), which was based on preceding cell biocompatibility studies. Nanoparticles prepared with different types of chitosan showed similar effect on the keratinocyte proliferation/migration. Nanoparticle-mediated interplay of chitosan and melatonin was shown to be crucial for improved wound epithelialisation.
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutics, Zagreb, Croatia.
Filip
F
Blažević
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutics, Zagreb, Croatia.
Tamara
T
Milekić
R&D, PLIVA Croatia Ltd., TEVA Group Member, Zagreb, Croatia.
Marieta Duvnjak
MD
Romić
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutics, Zagreb, Croatia.
Marina
M
Juretić
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutics, Zagreb, Croatia.
Ivan
I
Pepić
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutics, Zagreb, Croatia.
Jelena
J
Filipović-Grčić
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutics, Zagreb, Croatia.
Jasmina
J
Lovrić
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutics, Zagreb, Croatia. Electronic address: ahafner@pharma.hr.
Anita
A
Hafner
D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D007700:Kinetics; D054709:Lecithins / Q000737:chemistry; D008550:Melatonin / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D053758:Nanoparticles / Q000737:chemistry*; D062326:Re-Epithelialization / Q000187:drug effects*
D054709:Lecithins; D048271:Chitosan; D008550:Melatonin
Chitosan; Keratinocytes; Melatonin; Nanoparticles; Scratch test; Wound healing