Nanoscale bioactive glass activates osteoclastic differentiation of RAW 264.7 cells.
BACKGROUND
There is limited knowledge regarding differentiation of osteoclasts in the presence of nanoscale bioactive glass (nBG). This investigation examined increasing concentrations of 45S5 nBG and their influence on osteoclast differentiation.


METHODS
Different concentrations of 45S5 nBG were cultured up to 14 days with the murine RAW264.7 cell line and human primary monocytes cultured with M-CSF and RANKL.


RESULTS
Culturing cells for 14 days with 500 μg/ml nBG showed a viability of 100%; however DNA synthesis was reduced, supporting differentiation into osteoclast-like cells. Using RAW cells, activation of nine genes, including cell fusion genes, occurred in an nBG concentration dependent manner. Low concentrations of nBG increased expression of genes involved in commitment to cell fusion, whereas high concentrations increased gene expression supporting osteoclast-like differentiation.


CONCLUSIONS
nBG enhances both RAW264.7 and human osteoclast differentiation. nBG controlled gene expression in a concentration dependent manner could reflect normal regulation during bone growth.
Department of Materials Science & Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
Rainer
R
Detsch
Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
Matthias
M
Rübner
Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
Pamela L
PL
Strissel
Institute for Chemical & Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland.|Clinic of Preventive Dentistry, Periodontology & Cariology, University of Zurich, Center of Dental Medicine, Plattenstraße 11, 8032 Zurich, Switzerland.
Dirk
D
Mohn
Department of Transfusion Medicine & Haemostaseology, University-Clinic Erlangen, Krankenhausstraße 12, 91054 Erlangen, Germany.
Erwin
E
Strasser
Institute for Chemical & Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland.
Wendelin J
WJ
Stark
Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
Reiner
R
Strick
Department of Materials Science & Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
Aldo R
AR
Boccaccini
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005898:Glass; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects*; D000067996:RAW 264.7 Cells
D001672:Biocompatible Materials; C425330:bioactive glass 45S5
bioactive glass; biomaterials; bone tissue engineering; nanoparticles; osteoclasts