Drug-loading of poly(ethylene glycol methyl ether methacrylate) (PEGMEMA)-based micelles and mechanisms of uptake in colon carcinoma cells.
In this study polymeric micelles formed from poly(poly(ethylene glycol) methyl ether methacrylate)-block-poly(methyl methacrylate) (P(PEGMEMA75)-b-PMMA80) block copolymer of approximately 25nm in diameter were used to encapsulate the model drug, Nile Red, with a loading efficiency of 0.08wt% and a chemotherapeutic drug, doxorubicin (DOX), with an efficiency of 2.75wt%. The release of DOX from the micelles was sufficient to be cytotoxic to human colon carcinoma cells, WiDr, while Nile Red and the unloaded micelles were found not to be cytotoxic when exposed to the cells at polymer concentrations up to 200μg/mL. Nile Red loaded micelles were used to analyze uptake of the micelles into the cells which were rapidly internalized within minutes of exposure. The three major endocytotic pathways were involved in the internalization of micelles; however other passive mechanisms were also at play as the addition of inhibitors to all three pathways did not completely inhibit the uptake of these nanoparticles. These data demonstrate the potential of the P(PEGMEMA)75-b-PMMA80 block copolymer micelles to be rapidly internalized by carcinoma cells and deliver low doses of drugs intracellularly for controlled drug release.
Graduate School of Biomedical Engineering. University of New South Wales, Sydney, NSW 2052, Australia.
Teddy
T
Chang
Graduate School of Biomedical Engineering. University of New South Wales, Sydney, NSW 2052, Australia.
Pallavi
P
Gosain
Centre for Advanced Macromolecular Design, School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.
Martina H
MH
Stenzel
Graduate School of Biomedical Engineering. University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: m.lord@unsw.edu.au.
Megan S
MS
Lord
D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism*; D004317:Doxorubicin / Q000494:pharmacology*; D065546:Drug Liberation; D004705:Endocytosis / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D008823:Micelles; D010078:Oxazines / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry*
D008689:Methacrylates; D008823:Micelles; D010078:Oxazines; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D004317:Doxorubicin; C044808:nile red
Cancer cells; Cell uptake pathways; Doxorubicin; Drug release; Polymeric micelles