Biological and molecular characterization of a highly divergent johnsongrass mosaic virus isolate from Pennisetum purpureum.
The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence identity (86 % amino acid (aa) sequence identity) to a johnsongrass mosaic virus isolate from Australia. The coat protein (CP) identity values, however, were lower than those for the whole genome (78 % and 80 % for nt and aa, respectively) and were close to the species demarcation values (77 % nt and 80 % aa). Unexpectedly, the amino-terminal portion of CP of JGMV-CNPGL showed only 38 % sequence identity to other JGMV isolates. The biological implications of this sequence divergence remain to be elucidated.
Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil.
Karina N
KN
Silva
Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil.
Fernando L
FL
Melo
Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil.
Anelise F
AF
Orílio
Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil.
Tatsuya
T
Nagata
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil.
Marilia S
MS
Silva
Embrapa Beef Cattle, Campo Grande, Mato Grosso do Sul, Brazil.
Celso D
CD
Fernandes
Embrapa Cerrado, Planaltina, Distrito Federal, Brazil.
Rodrigo R
RR
Fragoso
Embrapa Cerrado, Planaltina, Distrito Federal, Brazil.
Suelen N
SN
Dessaune
Department of Cell Biology, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, 70910-900, Brazil. rresende@unb.br.
Renato O
RO
Resende
D000595:Amino Acid Sequence; D001483:Base Sequence; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D031782:Pennisetum / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins