Role of Arabidopsis NHL family in ABA and stress response.
Based on their sequence homology to Arabidopsis NDR1 and tobacco (Nicotiana tabacum) HIN1, 45 NHL (NDR1/HIN1-like) family genes are found in Arabidopsis genome. Recently, we reported that overexpression of NHL6, a member of NHL family, modulated seed germination under abiotic stresses through affecting ABA biosynthesis and signaling. We also carried out qPCR and investigated the expression of the other 8 member genes (NHL7a, 16, 17, 21, 25, 26, 41, 43) whose transcriptional data are publicly unavailable, and found that expression of NHL17 was induced more than 2 folds in ABA treated seedlings. Furthermore, in addition to the plasma membrane localization, YFP-NHL6 fusion protein was also observed in the cytosol (as dots) or on the membrane of small vacuoles or vesicles. As a member of the pathogen infection related genes, expression of NHL6 was significantly induced by salicylic acid and NHL6s are evolutionarily conserved among different plant species. A working model of NHL6 in ABA response was proposed.
a National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , China.|b Plant Sciences Institute and the Department of Genetics, Development and Cell Biology, Iowa State University , Ames , IA , USA.
Yan
Y
Bao
a National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , China.
Wei-Meng
WM
Song
a National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences , Shanghai , China.|c College of Agriculture, Ludong University , Yantai , China.
Hong-Xia
HX
Zhang
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D008954:Models, Biological; D005810:Multigene Family; D021381:Protein Transport / Q000187:drug effects; D020156:Salicylic Acid / Q000494:pharmacology; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D029681:Arabidopsis Proteins; D000040:Abscisic Acid; D020156:Salicylic Acid
ABA; NHL family; NHL6; abiotic stress; gene expression