Immunoprotection of recombinant Eg.P29 against Echinococcus granulosus in sheep.
OBJECTIVE
This study aims to investigate the immunoprotection of recombinant Eg.P29 (rEg.P29) vaccine and analyze the underlying mechanism in sheep.


METHODS
Three groups of male sheep were immunized subcutaneously with rEg.P29 and PBS, Freund's complete adjuvant as controls, respectively. After prime-boost vaccination, the sheep were challenged with encapsulated Echinococcus granulosus eggs. The percentage of protection in sheep was determined 36 weeks after the infection. Humoral immune response was analyzed for specific IgG, IgG1, IgG2, IgM and IgE levels. Moreover, cytokines including interferon (IFN)-γ, interleukin (IL)-2, IL-4,and IL-10 were also evaluated.


RESULTS
Immunization with rEg.P29 induced protective immune responses up to 94.5 %, compared with immunoadjuvant group. The levels of specific IgG, IgG1, IgG2, and IgE as well as IFN-γ, IL-2, and IL-4 significantly increased after two immunizations (P < 0.05); however, the levels of IgM and IL-10 did not show difference.


CONCLUSIONS
rEg.P29 showed Immunoprotection and induced Th1 and Th2 immune responses; hence, rEg.P29 is a potential vaccine for E. granulosus infection.
Department of Pathogenic Biology and Medical Immunology, Ningxia Medical University, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.|Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Hao
H
Wang
Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Zihua
Z
Li
Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.|Institute of Clinical Laboratory, Ningxia Medical University, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Fu
F
Gao
Department of Pathogenic Biology and Medical Immunology, Ningxia Medical University, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.|Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Jiaqing
J
Zhao
Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.|Centre of Scientific Technology of Ningxia Medical University, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Mingxing
M
Zhu
Department of Pathogenic Biology and Medical Immunology, Ningxia Medical University, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Xin
X
He
Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.|Institute of Clinical Laboratory, Ningxia Medical University, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Nan
N
Niu
Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China. zw-6915@163.com.
Wei
W
Zhao
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000097:blood; D004443:Echinococcosis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000276:immunology; D015047:Zoonoses / Q000517:prevention & control
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D014614:Vaccines, Synthetic
Echinococcus granulosus; Immunoprotection; Vaccine; rEg.P29