Comparison of the properties of collagen-chitosan scaffolds after γ-ray irradiation and carbodiimide cross-linking.
The property of collagen-chitosan porous scaffold varies according to cross-linking density and scaffold composition. This study was designed to compare the properties of collagen-chitosan porous scaffolds cross-linked with γ-irradiation and carbodiimide (CAR) for the first time. Eleven sets of collagen-chitosan scaffolds containing different concentrations of chitosan at a 5% increasing gradient were fabricated. Fourier transform infrared spectroscopy was performed to confirm the success of cross-linking in the scaffolds. The scaffold morphology was evaluated under scanning electron microscope (SEM). SEM revealed that chitosan was an indispensable material for the fabrication of γ-ray irradiation scaffold. The microstructure of γ-ray irradiation scaffold was less stable than those of alternative scaffolds. Based upon swelling ratio, porosity factor, and collagenase degradation, γ-ray irradiation scaffold was less stable than CAR and 25% proportion of chitosan scaffolds. Mechanical property determines the orientation in γ-irradiation and CAR scaffold. In vitro degradation test indicated that γ-irradiation and CAR cross-linking can elevate the scaffold biocompatibility. Compared with γ-ray irradiation, CAR cross-linked scaffold containing 25% chitosan can more significantly enhance the bio-stability and biocompatibility of collagen-chitosan scaffolds. CAR cross-linked scaffold may be the best choice for future tissue engineering.
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Zihao
Z
Chen
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Tianming
T
Du
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Xiangyu
X
Tang
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Changjun
C
Liu
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Ruixin
R
Li
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Cheng
C
Xu
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Feng
F
Tian
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Zhenjie
Z
Du
a Department of Biomedical Materials , Institute of Medical Equipment, Academy of Military Medical Sciences , Tianjin , China.
Jimin
J
Wu
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D002417:Cattle; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D017364:Collagenases / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D005720:Gamma Rays; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D013710:Tendons / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D003094:Collagen; D048271:Chitosan; D017364:Collagenases
Biomaterial; carbodiimide; cross-linking; mechanical property; porous materials; γ-ray irradiation