Effect of Chemical Profiling Change of Processed Magnolia officinalis on the Pharmacokinetic Profiling of Honokiol and Magnolol in Rats.
The stem of Magnoliae officinalis (MO) cortex is always preliminarily processed before being applied in traditional Chinese medicine. The definite bioavailability of honokiol (HO) and magnolol (MA) in processed MO (PMO) and the effect of chemical profiling change on the pharmacokinetics of HO and MA are always a greater challenge compared with those of MO. Compared with that of MO, the pharmacokinetic profiling of HO and MA in the PMO was significantly changed and the mean Tmax of HO and MA was increased by 31 and 50% (P < 0.05), respectively; the mean AUC0-t and Cmax of HO were increased by 36 and 24% (P < 0.05), respectively. Subsequently, the chemical profiling of MO and PMO was investigated by a simple and rapid LC-Q/TOF-MS coupled with multivariate analysis method. Principal component analysis and hierarchical cluster analysis of the chromatographic data demonstrated that the chemical profiling of PMO was significantly different from that of MO. Eight marker components including six alkaloids (magnocurarine, magnoflorine, roemerine and three unidentified peaks) and two lignans (obovatol and MA) were screened out by partial least-squares discriminant analysis. The results indicated that the changes of eight marker components of PMO may have an effect on the pharmacokinetic profiles of HO and MA.
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, PR China.
Huiling
H
Hu
Metabonomics Synergy Innovation Laboratory, School of Medicine and Nursing, Chengdu University, No.1, Shiling Street, Chengluo Road, Chengdu 610106, PR China wangzhanguo@scu.edu.cn.
Zhanguo
Z
Wang
National and Local Joint Engineering Laboratory for Energy Plant Bio-Oil Production and Application, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu, PR China.
Wan
W
Hua
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, PR China.
Yu
Y
You
Metabonomics Synergy Innovation Laboratory, School of Medicine and Nursing, Chengdu University, No.1, Shiling Street, Chengluo Road, Chengdu 610106, PR China.
Liang
L
Zou
D000470:Alkaloids / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D001713:Biphenyl Compounds / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006801:Humans; D017705:Lignans / Q000097:blood* / Q000493:pharmacokinetics; D031566:Magnolia / Q000737:chemistry*; D008297:Male; D015999:Multivariate Analysis; D018547:Plant Stems / Q000737:chemistry; D025341:Principal Component Analysis; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D000498:Allyl Compounds; D010636:Phenols
D000470:Alkaloids; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols