Bothrops erythromelas () venom induces apoptosis on renal tubular epithelial cells.
Bothrops erythromelas is responsible for a large number of snakebite incidents in Northeastern Brazil. Previously, we showed the effects of whole B. erythromelas venom in an isolated kidney model. To continue the study with B. erythromelas venom, the present work aims to study the effects of this venom on MDCK tubular epithelial cells and assess gene expression involved in kidney injury, aiming at elucidating the mechanisms responsible for renal toxicity. Cytotoxicity in MDCK cells showed an IC50 of 93 μg/mL and predominant apoptotic involvement demonstrated by flow cytometry assays and expression of caspase-3 and caspase-8. In conclusion, we suggest that Bothropoides erythromelas venom causes apoptosis with involvement of the caspases, probably through the extrinsic pathway.
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Fabíola Carine Monteiro
FC
de Sousa
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Antônio Rafael Coelho
AR
Jorge
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Ramon Róseo Paula Pessoa Bezerra
RR
de Menezes
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Alba Fabíola Costa
AF
Torres
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Clarissa Perdigão
CP
Mello
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Danya Bandeira
DB
Lima
Regional Nucleus of Ophiology (NUROF), Federal University of Ceara, Fortaleza, Ceara, Brazil.
Diva Maria
DM
Borges Nojosa
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Alexandre
A
Havt
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Renata Sousa
RS
Alves
Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. Electronic address: martinsalice@gmail.com.
Alice Maria Costa
AM
Martins
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Helena Serra Azul
HS
Monteiro
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D017837:Bothrops; D001938:Brazil; D053148:Caspase 3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003435:Crotalid Venoms / Q000201:enzymology / Q000633:toxicity*; D004285:Dogs; D005786:Gene Expression Regulation / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D007684:Kidney Tubules / Q000187:drug effects* / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D045726:Metalloproteases / Q000633:toxicity; D012333:RNA, Messenger / Q000378:metabolism; D030162:Reptilian Proteins / Q000819:agonists* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity
D003435:Crotalid Venoms; D012333:RNA, Messenger; D030162:Reptilian Proteins; D045726:Metalloproteases; D053148:Caspase 3; D053181:Caspase 8
Apoptosis; Bothropoides erythromelas venom; MDCK cells