Refolding effects of partially immiscible ammonium-based ionic liquids on the urea-induced unfolded lysozyme structure.
The activity of lysozyme over a Micrococcus lysodeikticus cell suspension increased to 13% of the initial value in the presence of 1% v/v ammonium-based ionic liquids after deactivation with 4.0 M urea. This increase in activity reflects the refolding ability of the ionic liquids against the denaturation effects of urea on lysozyme.
Department of Chemistry, University of Delhi, Delhi, 110007, India. venkatesup@hotmail.com pvenkatesu@chemistry.du.ac.in.
Meena
M
Bisht
Awanish
A
Kumar
Pannuru
P
Venkatesu
D064751:Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D052578:Ionic Liquids / Q000737:chemistry / Q000494:pharmacology*; D009113:Muramidase / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects*; D058849:Protein Refolding / Q000187:drug effects*; D014508:Urea / Q000494:pharmacology*
D064751:Ammonium Compounds; D052578:Ionic Liquids; D014508:Urea; D009113:Muramidase