Calmodulin Promotes N-BAR Domain-Mediated Membrane Constriction and Endocytosis.
Membrane remodeling by BAR (Bin, Amphiphysin, RVS) domain-containing proteins, such as endophilins and amphiphysins, is integral to the process of endocytosis. However, little is known about the regulation of endocytic BAR domain activity. We have identified an interaction between the yeast Rvs167 N-BAR domain and calmodulin. Calmodulin-binding mutants of Rvs167 exhibited defects in endocytic vesicle release. In vitro, calmodulin enhanced membrane tubulation and constriction by wild-type Rvs167 but not calmodulin-binding-defective mutants. A subset of mammalian N-BAR domains bound calmodulin, and co-expression of calmodulin with endophilin A2 potentiated tubulation in vivo. These studies reveal a conserved role for calmodulin in regulating the intrinsic membrane-sculpting activity of endocytic N-BAR domains.
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Margaret D
MD
Myers
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Sergey
S
Ryazantsev
Molecular Genetics and Microbiology, College of Natural Sciences, University of Texas at Austin, Austin, TX 78712, USA.
Linda
L
Hicke
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: gpayne@mednet.ucla.edu.
Gregory S
GS
Payne
D000818:Animals; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D003250:Constriction; D004705:Endocytosis / Q000235:genetics / Q000502:physiology*; D008081:Liposomes / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D002147:Calmodulin; D008081:Liposomes; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C082282:RVS167 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C075637:amphiphysin