Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells.
A highly sensitive microfluidic system to capture circulating tumor cells from whole blood of cancer patients is presented. The device incorporates graphene oxide into a thermoresponsive polymer film to serve as the first step of an antibody functionalization chemistry. By decreasing the temperature, captured cells may be released for subsequent analysis.
Department of Chemical Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.|Department of Electrical Engineering and Computer Science, South Dakota State University, Brookings, SD 57007, USA.|Biointerfaces Institute, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.|Translational Oncology Program, University of Michigan Health System, 1600 Huron Pkwy, Ann Arbor, MI 48109, USA.
Hyeun Joong
HJ
Yoon
Biointerfaces Institute, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.|Macromolecular Science and Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.
Apoorv
A
Shanker
Department of Chemical Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.|Biointerfaces Institute, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.|Translational Oncology Program, University of Michigan Health System, 1600 Huron Pkwy, Ann Arbor, MI 48109, USA.
Yang
Y
Wang
Department of Chemical Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.|Biointerfaces Institute, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.|Translational Oncology Program, University of Michigan Health System, 1600 Huron Pkwy, Ann Arbor, MI 48109, USA.
Molly
M
Kozminsky
Department of Chemical Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.
Qu
Q
Jin
Henry Ford Health System, 1 Ford Place, Detroit, MI 48202, USA.
Nallasivam
N
Palanisamy
Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.
Monika L
ML
Burness
Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.
Ebrahim
E
Azizi
Translational Oncology Program, University of Michigan Health System, 1600 Huron Pkwy, Ann Arbor, MI 48109, USA.|Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.
Diane M
DM
Simeone
Biointerfaces Institute, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.|Translational Oncology Program, University of Michigan Health System, 1600 Huron Pkwy, Ann Arbor, MI 48109, USA.|Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA.
Max S
MS
Wicha
Department of Chemical Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.|Biointerfaces Institute, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.|Macromolecular Science and Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.|Department of Material Science and Engineering, Department of Biomedical Engineering, Department of Chemistry, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.
Jinsang
J
Kim
Department of Chemical Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.|Biointerfaces Institute, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.|Translational Oncology Program, University of Michigan Health System, 1600 Huron Pkwy, Ann Arbor, MI 48109, USA.
Sunitha
S
Nagrath
D000906:Antibodies / Q000737:chemistry; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D044085:Microfluidics / Q000379:methods; D009369:Neoplasms / Q000097:blood / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010087:Oxides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
D000906:Antibodies; D010087:Oxides; D011108:Polymers; D006108:Graphite
breast cancer; circulating tumor cell; graphene oxide; pancreatic cancer; thermoresponsive polymer