Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.).
A water-soluble polysaccharide JS-MP-1 was isolated from Korean mulberry fruits Oddi (Morus alba L.). Sugar linkage analysis and NMR data confirmed that it is a rhamnogalacturonan type I (RG I) polymer carrying arabinan and arabinogalactan (AG II) side chains. JS-MP-1 reduced dose-dependently the viability of 3T3-L1 pre-adipocyte cells, significantly stimulated the cleavage of caspases 9 and 3 and poly (ADP-ribose) polymerase (PARP) and decreased the ratio of Bcl-2 to Bax expression level that led to mitochondrial dysfunction and apoptosis in pre-adipocyte cells. The apoptotic death was mediated by stimulation of MAPKs (ERK and p38) signalling pathway. These results suggest that JS-MP-1 is able to reduce the number of fat cells and the mass of adipose tissue via inhibition of pre-adipocyte proliferation and thus JS-MP-1 itself or a crude aqueous Oddi extract containing this polysaccharide can be used as functional ingredient of health-beneficial food supplements for the treatment or prevention of obesity disorders.
Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea, Republic of Korea.
Ji Won
JW
Choi
Department of Carbohydrates and Cereals, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Andriy
A
Synytsya
Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
Peter
P
Capek
Department of Carbohydrates and Cereals, University of Chemical Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Roman
R
Bleha
Institute of Organic Chemistry and Biochemistry AS CR, Flemingovo sq. 2, 166 28 Prague 6, Czech Republic.
Radek
R
Pohl
Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Republic of Korea, Republic of Korea. Electronic address: yongil382@catholic.ac.kr.
Yong Il
YI
Park
D016475:3T3 Cells; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000818:Animals; D019440:Anti-Obesity Agents / Q000737:chemistry / Q000302:isolation & purification; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D031625:Morus / Q000737:chemistry*; D009765:Obesity / Q000188:drug therapy; D010368:Pectins / Q000302:isolation & purification*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*
D019440:Anti-Obesity Agents; D011134:Polysaccharides; D010368:Pectins; D020928:Mitogen-Activated Protein Kinases
Apoptosis; Mulberry fruit; Pectic polysaccharide; Pre-adipocytes; Structure