Integrated sequence and immunology filovirus database at Los Alamos.
The Ebola outbreak of 2013-15 infected more than 28 000 people and claimed more lives than all previous filovirus outbreaks combined. Governmental agencies, clinical teams, and the world scientific community pulled together in a multifaceted response ranging from prevention and disease control, to evaluating vaccines and therapeutics in human trials. As this epidemic is finally coming to a close, refocusing on long-term prevention strategies becomes paramount. Given the very real threat of future filovirus outbreaks, and the inherent uncertainty of the next outbreak virus and geographic location, it is prudent to consider the extent and implications of known natural diversity in advancing vaccines and therapeutic approaches. To facilitate such consideration, we have updated and enhanced the content of the filovirus portion of Los Alamos Hemorrhagic Fever Viruses Database. We have integrated and performed baseline analysis of all family ITALIC! Filoviridaesequences deposited into GenBank, with associated immune response data, and metadata, and we have added new computational tools with web-interfaces to assist users with analysis. Here, we (i) describe the main features of updated database, (ii) provide integrated views and some basic analyses summarizing evolutionary patterns as they relate to geo-temporal data captured in the database and (iii) highlight the most conserved regions in the proteome that may be useful for a T cell vaccine strategy.Database URL:www.hfv.lanl.gov.
Los Alamos National Laboratory, Los Alamos, NM, USA kyusim@lanl.gov.
Karina
K
Yusim
Los Alamos National Laboratory, Los Alamos, NM, USA.
Hyejin
H
Yoon
Los Alamos National Laboratory, Los Alamos, NM, USA.
Brian
B
Foley
Los Alamos National Laboratory, Los Alamos, NM, USA.
Shihai
S
Feng
Los Alamos National Laboratory, Los Alamos, NM, USA.
Jennifer
J
Macke
Los Alamos National Laboratory, Los Alamos, NM, USA.
Mira
M
Dimitrijevic
Los Alamos National Laboratory, Los Alamos, NM, USA.
Werner
W
Abfalterer
Los Alamos National Laboratory, Los Alamos, NM, USA.
James
J
Szinger
Los Alamos National Laboratory, Los Alamos, NM, USA.
Will
W
Fischer
Los Alamos National Laboratory, Los Alamos, NM, USA.
Carla
C
Kuiken
Los Alamos National Laboratory, Los Alamos, NM, USA.
Bette
B
Korber
D030541:Databases, Genetic; D016563:Filoviridae / Q000235:genetics* / Q000276:immunology*; D018702:Filoviridae Infections / Q000276:immunology / Q000821:virology*; D006801:Humans; D020407:Internet; D009516:New Mexico; D014584:User-Computer Interface