In Vitro Shoot Cultures and Analysis of Steroidal Lactones in Withania coagulans (Stocks) Dunal.
Withania coagulans (Stocks) Dunal (Solanaceae), also known as 'Panir Bandh' is an important medicinal plant that is extensively used as a home remedy for several diseases in the Indian subcontinent. The plant possesses specific steroidal lactones known as withanolides which show high level of pharmaceutical activity against a broad spectrum of microorganisms. Natural propagation of the plant occurs through Seed but due to unisexual nature of the flowers; chances of Seed setting are very limited and the plant is on the verge of extinction because of overexploitation and reproductive failure. Plant tissue culture techniques offer opportunities for ex situ conservation and mass multiplication of endangered plant species through micropropagation and also enhancement of in vitro biosynthesis of bioactive compounds. In this chapter we present protocols for the mass multiplication of W. coagulans, assessment of clonal fidelity by RAPD, and estimation of bioactive compounds (withanolides) by thin layer chromatography (TLC) and reverse phase HPLC developed in our laboratory.
Department of Botany, University of Rajasthan, Jaipur, 302 004, India.|Department of Biosciences, Manipal University, Jaipur, 303 007, India.
Rohit
R
Jain
Department of Botany, University of Rajasthan, Jaipur, 302 004, India.
Sumita
S
Kachhwaha
Department of Botany, University of Rajasthan, Jaipur, 302 004, India. slkothari28@gmail.com.|Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, 303 002, India. slkothari28@gmail.com.
S L
SL
Kothari
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D007783:Lactones / Q000032:analysis* / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D046509:Tissue Culture Techniques / Q000379:methods*; D032341:Withania / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D054358:Withanolides / Q000032:analysis* / Q000378:metabolism
D007783:Lactones; D054358:Withanolides
Clonal fidelity; HPLC; Micropropagation; RAPD; Withania coagulans; Withanolides