A New Approach to Produce Resveratrol by Enzymatic Bioconversion.
An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 µg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.
College of Food Science and Engineering, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Jinxin
J
Che
Key laboratory for space biosciences and space biotechnology, School of life sciences, Northwestern polytechnical University, Xi'an, Shaanxi 710072, P.R. China.
Junling
J
Shi
College of Food Science and Engineering, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Zhenhong
Z
Gao
College of Food Science and Engineering, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Yan
Y
Zhang
D000464:Alginates; D000528:Alternaria / Q000201:enzymology*; D001711:Biotransformation; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000382:microbiology
D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D013267:Stilbenes; D020723:Glucuronic Acid; D005947:Glucose; D000077185:Resveratrol
Alternaria sp.; enzymatic synthesis; immobilized enzyme; resveratrol