Portable Enzyme-Paper Biosensors Based on Redox-Active CeO2 Nanoparticles.
Portable, nanoparticle (NP)-enhanced enzyme sensors have emerged as powerful devices for qualitative and quantitative analysis of a variety of analytes for biomedicine, environmental applications, and pharmaceutical fields. This chapter describes a method for the fabrication of a portable, paper-based, inexpensive, robust enzyme biosensor for the detection of substrates of oxidase enzymes. The method utilizes redox-active NPs of cerium oxide (CeO2) as a sensing platform which produces color in response to H2O2 generated by the action of oxidase enzymes on their corresponding substrates. This avoids the use of peroxidases which are routinely used in conjunction with glucose oxidase. The CeO2 particles serve dual roles, as high surface area supports to anchor high loadings of the enzyme as well as a color generation reagent, and the particles are recycled multiple times for the reuse of the biosensor. These sensors are small, light, disposable, inexpensive, and they can be mass produced by standard, low-cost printing methods. All reagents needed for the analysis are embedded within the paper matrix, and sensors stored over extended periods of time without performance loss. This novel sensor is a general platform for the in-field detection of analytes that are substrates for oxidase enzymes in clinical, food, and environmental samples.
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, New York, USA.
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Karimi
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, New York, USA.
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Othman
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, New York, USA. Electronic address: eandrees@clarkson.edu.
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Andreescu
D015374:Biosensing Techniques; D002563:Cerium / Q000737:chemistry; D057075:Enzyme Assays; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction
D004800:Enzymes, Immobilized; D002563:Cerium; C030583:ceric oxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
Ceria nanoparticles; Color-based measurements; Enzyme functionalized paper; Enzyme immobilization; Portable enzyme sensor; Redox nanoparticles