The potential immune modulatory effect of chronic bisphenol A exposure on gene regulation in male medaka (Oryzias latipes) liver.
Bisphenol A (BPA) is a well-known estrogenic endocrine disrupting chemical (EDC) ubiquitously present in various environmental media. The present study aims to identify the responsive genes in male fish chronically exposed to low concentrations of BPA at the transcription level. We screened genes from a suppression subtractive hybridization library constructed from male medaka (Oryzias latipes) livers after 60-d exposure to 10μg/L BPA under the condition at which changes of hepatic antioxidant parameters have been previously reported. The identified genes were predicted to be involved in multiple biological processes including antioxidant physiology, endocrine system, detoxification, notably associated with the immune response processes. With real time PCR analysis, the immune-associated genes including hepcidin-like precursor, complement component and factors, MHC class I, alpha-2-macroglobulin and novel immune-type receptor 6 isoform were significantly up-regulated in a nonmonotonic dose response pattern in livers upon exposure to different concentrations of BPA (0.1, 1, 10, 100, 1000μg/L). Our results demonstrated a negative impact on gene regulation in fish chronically exposed to relatively low and environmentally relevant concentrations of BPA, and suggested the potential immune modulatory effect of chronic EDC exposure on fish. The immunotoxicity of BPA and other EDCs should be much concerned for the health of human beings and other vertebrates exposed to it.
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Wenhui
W
Qiu
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Yang
Y
Shen
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Chenyuan
C
Pan
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Shuai
S
Liu
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.
Minghong
M
Wu
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China. Electronic address: mingyang@shu.edu.cn.
Ming
M
Yang
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian 361005, China. Electronic address: wkjian@xmu.edu.cn.
Ke-Jian
KJ
Wang
D000704:Analysis of Variance; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D018076:DNA, Complementary / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D004703:Endocrine System / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D007109:Immunity / Q000187:drug effects*; D007155:Immunologic Factors; D008099:Liver / Q000187:drug effects*; D008297:Male; D009990:Oryzias / Q000235:genetics / Q000378:metabolism*; D010636:Phenols / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D018076:DNA, Complementary; D052244:Endocrine Disruptors; D007155:Immunologic Factors; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Bisphenol A; Environmental estrogen; Gene expression; Immunotoxicity; Oryzias latipes