Improvement of Left Ventricular Remodelling by Inhibition of NF-κB in a Rat Model of Myocardial Infarction.
BACKGROUND
To investigate the effects of inhibition of NF-κB activation on left ventricular (LV) remodelling in a rat model of myocardial infarction (MI).


METHODS
The acute MI model was established by ligation of left anterior descending coronary artery. Pyrrolidine dithiocarbamate (PDTC) (20mg/kg, Qd) was administered intraperitoneally to inhibit NF-κB activation. Eight weeks later, the cardiac structure and LV ejection fraction were assessed with echocardiography. The rat body, heart, and LV weights were measured to calculate LV mass indices. Activation of NF-κB in non-infarcted myocardium was detected by a TransAM NF-κB p65 Transcription Factor Assay Kit. Cardiac collagen volume fraction was evaluated by Masson staining.


RESULTS
Eight weeks after the MI model was established, the LV posterior wall thickness in PDTC and MI group was 1.75±0.07mm and 1.85±0.07mm respectively (p<0.05). The LV mass index in the PDTC group (2.53±0.09) was lower than in the MI group (2.65±0.08, p<0.05). The LVEF in the PDTC group (63.89%±4.21%) was higher than in the MI group (42.73%±8.94%, p<0.05). The interstitial collagen deposition in the non-infarcted myocardium in the PDTC group was less than in the MI group (7.25%±1.88% vs. 10.09%±2.19%, p<0.05).


CONCLUSIONS
Inhibition of activation of NF-κB may result in improvement of myocardial remodelling after myocardial infarction, which is possibly attributable to reduced collagen deposition in non-infarcted areas.
Central Intensive Care Unit, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou 510220, Guangdong, China.
Jin-Lan
JL
Jin
Department of Cardiology, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou 510220, Guangdong, China.
Rong-Gui
RG
Lv
School of Pharmaceutical Science of Sun Yat-Sen University, Guangzhou 510080, Guangdong, China.
Jian
J
Guo
Department of Ultrasound, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou 510220, Guangdong, China.
Xi-Hong
XH
Liu
Central Intensive Care Unit, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou 510220, Guangdong, China.
Yan-Wen
YW
Liang
Department of Cardiology, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou 510220, Guangdong, China. Electronic address: jianruiwei2014@163.com.
Jian-Rui
JR
Wei
School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia. Electronic address: lwang@csu.edu.au.
Lexin
L
Wang
D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000503:physiopathology*; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D020257:Ventricular Remodeling
D016328:NF-kappa B
LV ejection fraction; LV mass index; Myocardial infarction; NF-κB; Ventricular remodelling