Driven Translocation of Polynucleotides Through an Aerolysin Nanopore.
Aerolysin has been used as a biological nanopore for studying peptides, proteins, and oligosaccharides in the past two decades. Here, we report that wild-type aerolysin could be utilized for polynucleotide analysis. Driven a short polynucleotide of four nucleotides length through aerolysin occludes nearly 50% amplitude of the open pore current. Furthermore, the result of total internal reflection fluorescence measurement provides direct evidence for the driven translocation of single polynucleotide through aerolysin.
Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology , Shanghai 200237, P. R. China.
Chan
C
Cao
Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology , Shanghai 200237, P. R. China.
Jie
J
Yu
Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology , Shanghai 200237, P. R. China.
Ya-Qian
YQ
Wang
Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology , Shanghai 200237, P. R. China.
Yi-Lun
YL
Ying
Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology , Shanghai 200237, P. R. China.
Yi-Tao
YT
Long
D001427:Bacterial Toxins / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques; D005452:Fluoresceins / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D011119:Polynucleotides / Q000032:analysis* / Q000737:chemistry; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry* / Q000648:ultrastructure
D001427:Bacterial Toxins; D004277:DNA, Single-Stranded; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011119:Polynucleotides; D052899:Pore Forming Cytotoxic Proteins; C032676:aerolysin