Structural biology applications of solid state MAS DNP NMR.
Dynamic Nuclear Polarization (DNP) has long been an aim for increasing sensitivity of nuclear magnetic resonance (NMR) spectroscopy, delivering spectra in shorter experiment times or of smaller sample amounts. In recent years, it has been applied in magic angle spinning (MAS) solid-state NMR to a large range of samples, including biological macromolecules and functional materials. New research directions in structural biology can be envisaged by DNP, facilitating investigations on very large complexes or very heterogeneous samples. Here we present a summary of state of the art DNP MAS NMR spectroscopy and its applications to structural biology, discussing the technical challenges and factors affecting DNP performance.
Aarhus Institute of Advanced Studies (AIAS), Aarhus University, Høegh-Guldbergs Gade 6B, 8000 Aarhus C, Denmark; Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark. Electronic address: akbey@inano.au.dk.
Ümit
Ü
Akbey
Leibniz Institute für Molekulare Pharmakologie (FMP), NMR Supported Structural Biology, Robert Roessle Str. 10, 13125 Berlin, Germany. Electronic address: oschkinat@fmp-berlin.de.
Hartmut
H
Oschkinat
D000465:Algorithms; D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
D001704:Biopolymers
Dynamic Nuclear Polarization (DNP); Hyperpolarization; Solid state MAS NMR; Structural biology