Effects of Organic Selenium Supplementation on Growth, Accumulation, Haematology and Histopathology of Juvenile Barramundi (Lates calcarifer) Fed High Soybean Meal Diets.
Soybean meal (SBM) has been commonly utilised as a substitute for fishmeal (FM) in the diets of several fish species. However, little is known regarding their effects on trace element availability and thus their importance to fish. The present study employed two feeding trials to evaluate the implications of dietary selenium (Se) on the growth, accumulation, antioxidant, and histopathological responses of juvenile barramundi (Lates calcarifer). In the first trial, each of three basal diets containing 0, 15 and 43 % SBM as replacements for 0, 25 and 75 % of FM protein on an isoproteic and isocalorific basis were either supplemented or not supplemented with 2 mg kg-1organic Se (OS). In the second trial, the potential effect of OS supplementation in a high SBM diet was investigated in a feeding trial with five experimental diets: 75 % SBM protein as replacement of FM was supplemented with 2, 3, 4, 5 or 7 mg OS kg-1. Growth was independently influenced by the SBM level and the OS supplementation level but not by their interaction. Glutathione peroxidase (GPx) activity, haematocrit, Se accumulation and muscle tissue integrity were significantly enhanced in fish fed on OS-supplemented diets. Furthermore, when high SBM was included in diets, elevated Se tended to lower the barramundi's performance. These findings suggest that dietary supplementation of OS at 2-3 g kg-1diet is necessary when high plant protein ingredients are incorporated in the diet, in order to maintain better growth and to afford protection against oxidative stress.
Department of Environment and Agriculture, Curtin University, 1 Turner Ave Technology Park, Bentley, WA, 6102, Australia. ilham@postgrad.curtin.edu.au.|Department of Aquatic Resources Management & Technology, Jakarta Fisheries University, Jl. AUP Pasar Minggu, Jakarta Selatan, 12520, Indonesia. ilham@postgrad.curtin.edu.au.
Ilham
I
Ilham
Department of Environment and Agriculture, Curtin University, 1 Turner Ave Technology Park, Bentley, WA, 6102, Australia.
Muhammad Abu Bakar
MA
Siddik
Department of Environment and Agriculture, Curtin University, 1 Turner Ave Technology Park, Bentley, WA, 6102, Australia.
Ravi
R
Fotedar
D000821:Animal Feed; D000818:Animals; D019587:Dietary Supplements; D005399:Fishes / Q000097:blood* / Q000254:growth & development*; D018384:Oxidative Stress / Q000187:drug effects; D012643:Selenium / Q000494:pharmacology*; D013025:Glycine max
D012643:Selenium
Barramundi; Glutathione peroxidase; Growth; Necrosis; Organic selenium; Soybean meal