[Isolation of microorganisms producing enzyme capable of degrading tobacco straw and nicotine].
OBJECTIVE
The aim of this study was to screen tobacco straw and nicotine degrading microorganism.


METHODS
The bacterium was isolated from tobacco field soil using medium containing tobacco straw as the sole carbon and nitrogen source. We identified the bacterium through morphological and physiological characterization combined with the result of 16S rRNA gene sequence and data analysis. We also studied the lignocelluloses degradation and enzyme activities related to the degradation of lignin and cellulose in liquid state fermentation of tobacco stalk.


RESULTS
The bacterium was identified as Bacillus megaterium and we had demonstrated that it has a good ability to degrade lignin in tobacco straw when fermented in liquid state. It showed the highest laccase production of 418. 52 U/L while the highest lignin peroxides and manganese peroxides activity was 19. 71 U/L and 64. 71 U/L. On the other hand, we also found that nicotine in tobacco stem was totally degraded 20 d after inoculation.


CONCLUSIONS
to the isolated Bacillus megaterium is capable of degrading tobacco straw partially and nicotine totally.
Yulong
Y
Su
Qian
Q
Wang
Chengsheng
C
Zhang
Jingang
J
Gu
Sujuan
S
Shi
S M Nuruzzaman
SM
Manik
Jingjing
J
Mao
Shigui
S
Li
Qiang
Q
Lei
Renjun
R
Wu
Ying
Y
Yin
Jiankang
J
Qu
Liang
L
Li
Haobao
H
Liu
D001410:Bacillus megaterium / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D009538:Nicotine / Q000378:metabolism*; D010802:Phylogeny; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology*; D012988:Soil Microbiology; D014026:Nicotiana / Q000378:metabolism / Q000382:microbiology*
D001426:Bacterial Proteins; D009538:Nicotine; D002482:Cellulose; D008031:Lignin