A upconversion luminescene biosensor based on dual-signal amplification for the detection of short DNA species of c-erbB-2 oncogene.
High-sensitivity detection of trace amounts of c-erbB-2 oncogene was reported to be equal to or surpass the ability of CA 15-3 for early diagnosis and/or follow-up recurrent screening of breast cancer. Therefore, in the current study, by using upconversion nanoparticles (UCNPs), rare earth-doped NaYF4:Yb(3+)/Er(3+) as the luminescent labels, a upconversion luminescent (UCL) biosensor based on dual-signal amplification of exonuclease III (ExoIII)-assisted target cycles and long-range self-assembly DNA concatamers was developed for the detection of c-erbB-2 oncogene. The proposed biosensor exhibited ultrasensitive detection with limit as low as 40 aM, which may express the potential of being used in trace analysis of c-erbB-2 oncogene and early diagnosis of breast cancer.
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Jianming
J
Lan
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Yingxin
Y
Liu
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Li
L
Li
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Fadi
F
Wen
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Fang
F
Wu
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Zhizhong
Z
Han
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Weiming
W
Sun
Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Chunyan
C
Li
Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, P. R. China.
Jinghua
J
Chen
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D018734:Genes, erbB-2; D006801:Humans; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004247:DNA