Deproteinization of water-soluble ß-glucan during acid extraction from fruiting bodies of Pleurotus ostreatus mushrooms.
Some ß-glucans can be easily extracted from Basidiomycete mushrooms but commonly used extraction procedures are not satisfactory. A simultaneous method for acid extraction and deproteinization in the case of Pleurotus ostreatus was developed using response surface methodology. The optimized extraction conditions proposed here (30°C, 3.8% HCl, 300min, stirring) allow for the simultaneous extraction and deproteinization of polysaccharides. Additionally, the acid extraction yield was 7 times greater than that of hot water extraction. The combined enzymatic digestion with lyticase, ß-glucanase, exo-1,3-ß-d-glucanase, and ß-glucosidase results elucidated that an extract containing ß-1,3-ß-1,6-ß-1,4-glucan. The gel permeation chromatography (GPC) results showed that the two glucan fractions obtained do not contain linked proteins. The weight average molecular weight of the first fraction (Mw=1137kDa) was 60 times higher than that of the second fraction (Mw=19kDa).
Department of Food Science and Nutrition, Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland. Electronic address: artursz@up.poznan.pl.
Artur
A
Szwengiel
Department of Food Science and Nutrition, Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland. Electronic address: bstach@up.poznan.pl.
Barbara
B
Stachowiak
D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005656:Fungal Proteins / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism
D005656:Fungal Proteins; D047071:beta-Glucans; D014867:Water
Acid extraction; Deproteinization; Pleurotus ostreatus; ß-glucan