Chemical characterization and immunomodulatory properties of polysaccharides isolated from probiotic Lactobacillus casei LOCK 0919.
The Lactobacillus casei strain, LOCK 0919, is intended for the dietary management of food allergies and atopic dermatitis (LATOPIC®BIOMED). The use of a probiotic to modulate immune responses is an interesting strategy for solving imbalance problems of gut microflora that may lead to various disorders. However, the exact bacterial signaling mechanisms underlying such modulations are still far from being understood. Here, we investigated variations in the chemical compositions and immunomodulatory properties of the polysaccharides (PS), L919/A and L919/B, which are produced by L. casei LOCK 0919. By virtue of their chemical features, such PS can modulate the immune responses to third-party antigens. Our results revealed that L919/A and L919/B could both modulate the immune response to Lactobacillus planatarum WCFS1, but only L919/B could alter the response of THP-1 cells (in terms of tumor necrosis factor alpha production) to L. planatarum WCFS1 and Escherichia coli Nissle 1917. The comprehensive immunochemical characterization is crucial for the understanding of the biological function as well as of the bacteria-host and bacteria-bacteria cross-talk.
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland, sabina.gorska@iitd.pan.wroc.pl.
Sabina
S
Górska
Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v. v. i., 549 22 Novy Hradek, Czech Republic.
Petra
P
Hermanova
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
Jarosław
J
Ciekot
Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v. v. i., 549 22 Novy Hradek, Czech Republic.
Martin
M
Schwarzer
Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v. v. i., 549 22 Novy Hradek, Czech Republic.
Dagmar
D
Srutkova
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
Ewa
E
Brzozowska
Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v. v. i., 549 22 Novy Hradek, Czech Republic.
Hana
H
Kozakova
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
Andrzej
A
Gamian
D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007155:Immunologic Factors / Q000737:chemistry* / Q000276:immunology / Q000627:therapeutic use; D007780:Lacticaseibacillus casei / Q000737:chemistry* / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology; D019936:Probiotics / Q000627:therapeutic use
D007155:Immunologic Factors; D011134:Polysaccharides
Lactobacillus; NMR spectroscopy; immunomodulation; polysaccharide; probiotic