CDC-25.2, a C. elegans ortholog of cdc25, is essential for the progression of intestinal divisions.
Intestinal divisions in Caenorhabditis elegans take place in 3 stages: (1) cell divisions during embryogenesis, (2) binucleations at the L1 stage, and (3) endoreduplications at the end of each larval stage. Here, we report that CDC-25.2, a C. elegans ortholog of Cdc25, is required for these specialized division cycles between the 16E cell stage and the onset of endoreduplication. Results of our genetic analyses suggest that CDC-25.2 regulates intestinal cell divisions and binucleations by counteracting WEE-1.3 and by activating the CDK-1/CYB-1 complex. CDC-25.2 activity is then repressed by LIN-23 E3 ubiquitin ligase before the onset of intestinal endoreduplication, and this repression is maintained by LIN-35, the C. elegans ortholog of Retinoblastoma (Rb). These findings indicate that timely regulation of CDC-25.2 activity is essential for the progression of specialized division cycles and development of the C. elegans intestine.
a Department of Bioscience and Biotechnology , Konkuk University , Seoul , South Korea.
Yong-Uk
YU
Lee
a Department of Bioscience and Biotechnology , Konkuk University , Seoul , South Korea.
Miseol
M
Son
a Department of Bioscience and Biotechnology , Konkuk University , Seoul , South Korea.|b Current address: Laboratory of Genetics, BRC, National Institutes of Health, National Institute on Aging , Baltimore , MD , USA.
Jiyoung
J
Kim
a Department of Bioscience and Biotechnology , Konkuk University , Seoul , South Korea.
Yhong-Hee
YH
Shim
a Department of Bioscience and Biotechnology , Konkuk University , Seoul , South Korea.|c Institute of KU Biotechnology, Konkuk University , Seoul , South Korea.
Ichiro
I
Kawasaki
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D002455:Cell Division; D002467:Cell Nucleus / Q000502:physiology; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D007422:Intestines / Q000166:cytology / Q000196:embryology*; D008297:Male; D010749:Phosphoprotein Phosphatases / Q000502:physiology*
D029742:Caenorhabditis elegans Proteins; D010749:Phosphoprotein Phosphatases; C550003:cdc-25.2 protein, C elegans
Binucleation; C. elegans; cdc-25.1; cdc-25.2; cell-cycle regulation; endoreduplication; intestinal division; lin-23; lin-35; nuclear division