High HIV-1 Genetic Diversity in Patients from Northern Brazil.
The HIV-1 epidemic in Brazil is driven by subtypes B, F1, and C and recombinants forms among those subtypes. The distribution of HIV-1 subtypes, however, may vary across different Brazilian regions and the molecular epidemiologic profile in Northern Brazil remains poorly explored. HIV-1 pol sequences were obtained from 305 patients failing antiretroviral therapy followed at outpatient clinics from five Northern Brazilian states. The most prevalent HIV-1 clade observed in the Northern Brazilian region was subtype B (81%), followed by BF1 recombinants (10%), subtype F1 (4%), subtype C (3%), BC recombinants (2%), and BU recombinants (1%). Although HIV-1 subtype B was the predominant HIV-1 clade in Northern Brazil, its prevalence greatly varies among different states, ranging from 63% in Rondônia to 92% in Acre. Among the 37 HIV-1 recombinant sequences detected in the Northern Brazilian region, nine (24%) displayed a unique recombinant form structure, five (14%) a CRF28/29_BF-like structure, and four (11%) a CRF31_BC-like structure. Two other BF1 recombinant patterns were identified in 16 (43%) and three (8%) samples that may correspond to two potentially new CRFs_BF characteristic of the Northern region. This study reveals that despite the low spatial connectivity with other Brazilian regions, the genetic complexity of the HIV-1 epidemic in Northern Brazil is very high and that the molecular epidemiologic pattern may vary across different northern states, reflecting a complex epidemic with multiple independent viral introductions into this Brazilian region.
1 Laboratório de Genotipagem de HIV , Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Secretaria de Estado da Saúde. Governo do Estado do Amazonas, Manaus, Brazil .|2 Universidade do Estado do Amazonas , Manaus, Brazil .
Carolina Marinho
CM
da Costa
1 Laboratório de Genotipagem de HIV , Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Secretaria de Estado da Saúde. Governo do Estado do Amazonas, Manaus, Brazil .
Cintia Mara
CM
Costa de Oliveira
1 Laboratório de Genotipagem de HIV , Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Secretaria de Estado da Saúde. Governo do Estado do Amazonas, Manaus, Brazil .
Yonne Francis
YF
Chehuan de Melo
3 Laboratório de AIDS & Imunologia Molecular, Instituto Oswaldo Cruz , Fiocruz, Rio de Janeiro, Brazil .
Edson
E
Delatorre
3 Laboratório de AIDS & Imunologia Molecular, Instituto Oswaldo Cruz , Fiocruz, Rio de Janeiro, Brazil .
Gonzalo
G
Bello
3 Laboratório de AIDS & Imunologia Molecular, Instituto Oswaldo Cruz , Fiocruz, Rio de Janeiro, Brazil .
Jose Carlos
JC
Couto-Fernandez
D001938:Brazil / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D015995:Prevalence; D017422:Sequence Analysis, DNA; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D054302:pol Gene Products, Human Immunodeficiency Virus