Aluminium and Acrylamide Disrupt Cerebellum Redox States, Cholinergic Function and Membrane-Bound ATPase in Adult Rats and Their Offspring.
Accumulation of aluminium and acrylamide in food is a major source of human exposure. Their adverse effects are well documented, but there is no information about the health problems arising from their combined exposure. The aim of the present study was to examine the possible neurotoxic effects after co-exposure of pregnant and lactating rats to aluminium and acrylamide in order to evaluate redox state, cholinergic function and membrane-bound ATPases in the cerebellum of adult rats and their progeny. Pregnant female rats have received aluminium (50 mg/kg body weight) via drinking water and acrylamide (20 mg/kg body weight) by gavage, either individually or in combination from the 14th day of pregnancy until day 14 after delivery. Exposure to these toxicants provoked an increase in malondialdehyde (MDA) and advanced oxidation protein product (AOPP) levels and a decrease in SOD, CAT, GPx, Na+K+-ATPase, Mg2+-ATPase and AChE activities in the cerebellum of mothers and their suckling pups. A reduction in GSH, NPSH and vitamin C levels was also observed. These changes were confirmed by histological results. Interestingly, co-exposure to these toxicants exhibited synergism based on physical and biochemical variables in the cerebellum of mothers and their progeny.
Animal Physiology Laboratory, LR 11/ ES-53 Sciences Faculty of Sfax, University of Sfax, BP 1171, 3000, Sfax, Tunisia.
Imen
I
Ghorbel
Animal Physiology Laboratory, LR 11/ ES-53 Sciences Faculty of Sfax, University of Sfax, BP 1171, 3000, Sfax, Tunisia.
Ibtissem Ben
IB
Amara
Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax-Ecole Nationale d'Ingénieurs de Sfax, 3038, Sfax, Tunisia.
Naourez
N
Ktari
Animal Physiology Laboratory, LR 11/ ES-53 Sciences Faculty of Sfax, University of Sfax, BP 1171, 3000, Sfax, Tunisia.
Awatef
A
Elwej
Anatomopathology Laboratory, CHU Habib Bourguiba, University of Sfax, 3029, Sfax, Tunisia.
Ons
O
Boudawara
Anatomopathology Laboratory, CHU Habib Bourguiba, University of Sfax, 3029, Sfax, Tunisia.
Tahia
T
Boudawara
Animal Physiology Laboratory, LR 11/ ES-53 Sciences Faculty of Sfax, University of Sfax, BP 1171, 3000, Sfax, Tunisia. naj_zgh@yahoo.fr.
Najiba
N
Zeghal
D000109:Acetylcholine / Q000378:metabolism*; D020106:Acrylamide / Q000633:toxicity*; D062107:Advanced Oxidation Protein Products / Q000378:metabolism; D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D002531:Cerebellum / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018811:Maternal Exposure / Q000009:adverse effects*; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats
D062107:Advanced Oxidation Protein Products; D020106:Acrylamide; D008315:Malondialdehyde; D000535:Aluminum; D010088:Oxidoreductases; D000109:Acetylcholine
Acrylamide; Adult and suckling rats; Aluminium chloride; Cerebellum; Oxidative stress