Direct and indirect effects of RNA interference against pyridoxal kinase and pyridoxine 5'-phosphate oxidase genes in Bombyx mori.
Vitamin B6 comprises six interconvertible pyridine compounds (vitamers), among which pyridoxal 5'-phosphate is a coenzyme involved in a high diversity of biochemical reactions. Humans and animals obtain B6 vitamers from diet, and synthesize pyridoxal 5'-phosphate by pyridoxal kinase and pyridoxine 5'-phosphate oxidase. Currently, little is known on how pyridoxal 5'-phosphate biosynthesis is regulated, and pyridoxal 5'-phosphate is supplied to meet their requirement in terms of cofactor. Bombyx mori is a large silk-secreting insect, in which protein metabolism is most active, and the vitamin B6 demand is high. In this study, we successfully down-regulated the gene expression of pyridoxal kinase and pyridoxine 5'-phosphate oxidase by body cavity injection of synthesized double-stranded small interfering RNA to 5th instar larvae of Bombyx mori, and analyzed the gene transcription levels of pyridoxal 5'-phosphate dependent enzymes, phosphoserine aminotransferase and glutamic-oxaloacetic transaminase. Results show that the gene expression of pyridoxal kinase and pyridoxine 5'-phosphate oxidase has a greater impact on the gene transcription of enzymes using pyridoxal 5'-phosphate as a cofactor in Bombyx mori. Our study suggests that pyridoxal 5'-phosphate biosynthesis and dynamic balance may be regulated by genetic networks.
School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, People's Republic of China.
ShuoHao
S
Huang
School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, People's Republic of China.
LiLi
L
Yao
School of Foreign Languages, Anhui Agricultural University, Hefei 230036, People's Republic of China.
JianYun
J
Zhang
School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, People's Republic of China. Electronic address: lqhuang218@aliyun.com.
LongQuan
L
Huang
D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics; D053898:Biosynthetic Pathways; D012831:Bombyx / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D053263:Gene Regulatory Networks; D007814:Larva / Q000235:genetics / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011734:Pyridoxaminephosphate Oxidase / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D000637:Transaminases / Q000235:genetics; D014158:Transcription, Genetic; D059467:Transcriptome; D014815:Vitamins / Q000096:biosynthesis*
D034741:RNA, Small Interfering; D014815:Vitamins; D011732:Pyridoxal Phosphate; D011734:Pyridoxaminephosphate Oxidase; D000637:Transaminases; D001219:Aspartate Aminotransferases; C022393:phosphoserine aminotransferase; C024593:pyridoxine 5-phosphate
Bombyx mori; Glutamic-oxaloacetic transaminase; Phosphoserine aminotransferase; Pyridoxal kinase; Pyridoxine 5′-phosphate oxidase; RNA interference