Lysozyme-loaded lipid-polymer hybrid nanoparticles: preparation, characterization and colloidal stability evaluation.
BACKGROUND
Lipid-polymer hybrid nanoparticles (LPNPs) are polymeric nanoparticles enveloped by lipid layers, which have emerged as a potent therapeutic nanocarrier alternative to liposomes and polymeric nanoparticles.


OBJECTIVE
The aim of this work was to develop, characterize and evaluate LPNPs to deliver a model protein, lysozyme.


METHODS
Lysozyme-loaded LPNPs were prepared by using the modified w/o/w double-emulsion-solvent-evaporation method. Poly-ɛ-caprolactone (PCL) was used as polymeric core material and tripalmitin:lechitin mixture was used to form a lipid shell around the LPNPs. LPNPs were evaluated for particle size distribution, zeta potential, morphology, encapsulation efficiency, in vitro drug release, stability and cytotoxicity.


RESULTS
The DLS measurement results showed that the particle size of LPNPs ranged from 58.04 ± 1.95 nm to 2009.00 ± 0.52 nm. The AFM and TEM images of LPNPs demonstrate that LPNPs are spherical in shape. The protein-loading capacity of LPNPs ranged from 5.81% to 60.32%, depending on the formulation parameters. LPNPs displayed a biphasic drug release pattern with a burst release within 1 h, followed by sustained release afterward. Colloidal stability results of LPNPs in different media showed that particle size and zeta potential values of particles did not change significantly in all media except of FBS 100% for 120 h. Finally, the results of a cellular uptake study showed that LPNPs were significantly taken up by 83.3% in L929 cells.


CONCLUSIONS
We concluded that the LPNPs prepared with PCL as polymeric core material and tripalmitin:lechitin mixture as lipid shell should be a promising choice for protein delivery.
a Department of Pharmaceutical Technology, Faculty of Pharmacy , Ankara University , Ankara , Turkey ;
Burcu
B
Devrim
b Department of Biology, Faculty of Art and Science , Hitit University , Çorum, Turkey ;
Aslı
A
Kara
c Department of Pharmaceutical Technology, Faculty of Pharmacy , Hacettepe University , Ankara , Turkey.
İmran
İ
Vural
a Department of Pharmaceutical Technology, Faculty of Pharmacy , Ankara University , Ankara , Turkey ;
Asuman
A
Bozkır
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000493:pharmacokinetics; D002208:Caproates / Q000737:chemistry*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D004655:Emulsions; D006801:Humans; D007783:Lactones / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
D000970:Antineoplastic Agents; D002208:Caproates; D004655:Emulsions; D007783:Lactones; D008055:Lipids; D008081:Liposomes; D011108:Polymers; D012997:Solvents; D011092:Polyethylene Glycols; C121056:caprolactone; D009113:Muramidase
Colloidal stability; lipid-polymer hybrid nanoparticle; poly-ɛ-caprolactone; protein delivery; tripalmitin