[Determination of N acetyl-S-(2-carbamoylethyl)-cysteine in urine by high-performance liquid chromatography].
OBJECTIVE
To establish the method of high-performance liquid chromatography HPLC for the determination of N-acetyl-S-(2-carbamoylethyl)-cysteine (AAMA) in urine.


METHODS
After acid hydrolysis, AAMA in urine was converted into S-2-carboxyethyl cysteine (CEC). CEC reacted with the derivative reagent ophthalaldehyde and formed the derivative with strong fluorescence absorption. The HPLC-fluorescence detector was applied, with an excitation wavelength of 340 nm and an emission wavelength of 450 nm.


RESULTS
Urinary AAMA demonstrated an excellent linearity in the range of 5.3~123.5 μmol/L, with a correlation coefficient of 0.9994. The minimum detectable concentration was 0.1 μmol/L (the volume of urine sample was 1.0 ml), the recovery of standard addition was 97.4%~104.2%, and the between-run precision was 2.3%~4.3%. The sample could be stored in the refrigerator for at least 7 days at a temperature of 4℃.


CONCLUSIONS
The method is simple, with a low cost, a high sensitivity, and good precision and accuracy, and the instrument and equipment commonly seen in laboratories are applied. Therefore, this method is worthy of wide application.
School of Public Health, Sun Yat-sen University, Guangzhou 510620, China.
Shihua
S
Wu
Jiaming
J
Guo
Jiu
J
Chen
Wenfeng
W
Zeng
Yimin
Y
Liu
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans
C569465:N-acetyl-S-(2-carbamoylethyl)cysteine; D000111:Acetylcysteine