Surface heat shock protein 90 serves as a potential receptor for calcium oxalate crystal on apical membrane of renal tubular epithelial cells.
Adhesion of calcium oxalate monohydrate (COM) crystals on renal tubular epithelial cells is a crucial step in kidney stone formation. Finding potential crystal receptors on the apical membrane of the cells may lead to a novel approach to prevent kidney stone disease. Our previous study identified a large number of crystal-binding proteins on the apical membrane of MDCK cells. However, their functional role as potential crystal receptors had not been validated. The present study aimed to address the potential role of heat shock protein 90 (HSP90) as a COM crystal receptor. The apical membrane was isolated from polarized MDCK cells by the peeling method and recovered proteins were incubated with COM crystals. Western blot analysis confirmed the presence of HSP90 in the apical membrane and the crystal-bound fraction. Immunofluorescence staining without permeabilization and laser-scanning confocal microscopy confirmed the surface HSP90 expression on the apical membrane of the intact cells. Crystal adhesion assay showed that blocking surface HSP90 by specific anti-HSP90 antibody and knockdown of HSP90 by small interfering RNA (siRNA) dramatically reduced crystal binding on the apical surface of MDCK cells (by approximately 1/2 and 2/3, respectively). Additionally, crystal internalization assay revealed the presence of HSP90 on the membrane of endocytic vesicle containing the internalized COM crystal. Moreover, pretreatment of MDCK cells with anti-HSP90 antibody significantly reduced crystal internalization (by approximately 1/3). Taken together, our data indicate that HSP90 serves as a potential receptor for COM crystals on the apical membrane of renal tubular epithelial cells and is involved in endocytosis/internalization of the crystals into the cells.
Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Center for Research in Complex Systems Science, Mahidol University, 6th Floor-SiMR Building, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand.
Kedsarin
K
Fong-Ngern
Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Center for Research in Complex Systems Science, Mahidol University, 6th Floor-SiMR Building, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand.
Kanyarat
K
Sueksakit
Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Center for Research in Complex Systems Science, Mahidol University, 6th Floor-SiMR Building, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand. thongboonkerd@dr.com.
Visith
V
Thongboonkerd
D000818:Animals; D002129:Calcium Oxalate / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology*; D061985:Madin Darby Canine Kidney Cells; D013499:Surface Properties
D018841:HSP90 Heat-Shock Proteins; D002129:Calcium Oxalate
Apical membrane; Calcium oxalate; Crystal adhesion; Crystal binding; Crystal receptor