Enantiorecognition of planar "metallocenic" chirality by a nitrile hydratase/amidase bienzymatic system.
For the first time, a bacterial strain expressing a nitrile hydratase/amidase activity was able to recognize a planar element of chirality: Rhodococcus rhodochrous PA-34 whole cells catalysed with a high level of enantioselectivity the biotransformation of a novel nitrile ferrocene derivative into its corresponding amide and/or acid. An important parameter in the enzymatic recognition is the choice of the inducer selected for the bacterial growth phase.
CNR National Research Council of Italy - Institute of Biomolecular Chemistry, Via P-Gaifami 18, Catania, Italy. nicola.dantona@icb.cnr.it.
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D'Antona
CNR National Research Council of Italy - Institute of Biomolecular Chemistry, Via P-Gaifami 18, Catania, Italy. nicola.dantona@icb.cnr.it.
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Morrone
CNR National Research Council of Italy - Institute of Biomolecular Chemistry, Via P-Gaifami 18, Catania, Italy. nicola.dantona@icb.cnr.it.
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Gambera
CNR National Research Council of Italy - Institute of Biomolecular Chemistry, Via P-Gaifami 18, Catania, Italy. nicola.dantona@icb.cnr.it.
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Pedotti
D000581:Amidohydrolases / Q000378:metabolism*; D055162:Biocatalysis; D001711:Biotransformation; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism*; D000075163:Metallocenes / Q000737:chemistry / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology; D013237:Stereoisomerism; D013379:Substrate Specificity
D005296:Ferrous Compounds; D000075163:Metallocenes; D000581:Amidohydrolases; C019665:amidase; D006836:Hydro-Lyases; C048831:nitrile hydratase; C004998:ferrocene