Diphenylalanine peptide nanotubes self-assembled on functionalized metal surfaces for potential application in drug-eluting stent.
This study focuses on the potential of diphenylalanine self-assembled peptide nanotubes (FF Nts) for delivery of flufenamic acid (FA) from metal implants. Self-assembly of FF Nts was studied in solution and on surfaces of glass, silicone and gold substrates. FA was loaded inside the shell of FF Nts and subsequently FF/FA Nts were attached to gold surfaces. The substrate were characterized by Field Emission Scanning Electron Microscopy (FESEM), fluorescence microscopy, confocal microscopy, and UV-vis spectroscopy. Release of FA from FF Nts were investigated by immersing coated metal substrates in phosphate-buffered saline for 12 days. Self-assembly of FF in water and solvent resulted in formation of nanotubes, which efficiently loaded 98% of FA with concentration of 20 µg/mL. FESEM images confirmed successful attachment of FF/FA Nts to functionalized gold substrates. In vitro release studies indicated using FF Nts has prolonged the release rate of FA for several days. Biocompatibility studied confirmed more than 50% of the cells were alive in concentration of 250-1000 µg/mL of FF Nts thus suggesting the potential of peptide based self-assemble nanostructures as an alternate system for polymer coating in drugs eluting stents. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2280-2290, 2016.
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Iran.
Tayebeh
T
Zohrabi
Nanotechnology and Advanced Material Institute, Isfahan University of Technology, Iran.
Neda
N
Habibi
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Iran.
Ali
A
Zarrabi
Department of Biology, Faculty of Science, University of Isfahan, Iran.
Maryam
M
Fanaei
School of Chemical Engineering and Advanced Materials, Newcastle University, United Kingdom.
Lai Yeng
LY
Lee
D004151:Dipeptides; D054855:Drug-Eluting Stents; D005439:Flufenamic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D008422:Materials Testing; D045333:Nanotubes, Peptide / Q000737:chemistry*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry
D004151:Dipeptides; D045333:Nanotubes, Peptide; C026650:phenylalanylphenylalanine; D010649:Phenylalanine; D005439:Flufenamic Acid
di-phenylalanine; drug-eluting stents; flufenamic acid; self-assemble peptides