The Pathogenic A116V Mutation Enhances Ion-Selective Channel Formation by Prion Protein in Membranes.
Prion diseases are a group of fatal neurodegenerative disorders that afflict mammals. Misfolded and aggregated forms of the prion protein (PrP(Sc)) have been associated with many prion diseases. A transmembrane form of PrP favored by the pathogenic mutation A116V is associated with Gerstmann-Sträussler-Scheinker syndrome, but no accumulation of PrP(Sc) is detected. However, the role of the transmembrane form of PrP in pathological processes leading to neuronal death remains unclear. This study reports that the full-length mouse PrP (moPrP) significantly increases the permeability of living cells to K(+), and forms K(+)- and Ca(2+)-selective channels in lipid membranes. Importantly, the pathogenic mutation A116V greatly increases the channel-forming capability of moPrP. The channels thus formed are impermeable to sodium and chloride ions, and are blocked by blockers of voltage-gated ion channels. Hydrogen-deuterium exchange studies coupled with mass spectrometry (HDX-MS) show that upon interaction with lipid, the central hydrophobic region (109-132) of the protein is protected against exchange, making it a good candidate for inserting into the membrane and lining the channel. HDX-MS also shows a dramatic increase in the protein-lipid stoichiometry for A116V moPrP, providing a rationale for its increased channel-forming capability. The results suggest that ion channel formation may be a possible mechanism of PrP-mediated neurodegeneration by the transmembrane forms of PrP.
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bengaluru, India.
Ambadi Thody
AT
Sabareesan
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bengaluru, India.
Jogender
J
Singh
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bengaluru, India; Biocon Bristol Myers Squibb Research Center, Bengaluru, India; School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) University, Bhubaneswar, India.
Samrat
S
Roy
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bengaluru, India. Electronic address: jayant@ncbs.res.in.
Jayant B
JB
Udgaonkar
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bengaluru, India. Electronic address: mathew@ncbs.res.in.
M K
MK
Mathew
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D010539:Permeability; D011188:Potassium / Q000378:metabolism; D000072002:Prion Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000072417:Protein Domains
D007473:Ion Channels; D000072002:Prion Proteins; D011188:Potassium; D002118:Calcium