Mitochondria-Targeted Reaction-Based Fluorescent Probe for Hydrogen Sulfide.
In this study, we developed a turn-on mitochondria-targeting hydrogen sulfide, "probe 1", based on the selective thiolysis of 7-nitro-1,2,3-benzoxadiazole amine moiety attached to the piperazine-based naphthalimide scaffold. Probe 1 exhibited excellent properties with 68-fold fluorescence enhancement, a low detection limit (2.46 μM), a low cytotoxicity, and a good selectivity toward hydrogen sulfide. The success of intracellular imaging indicated that probe 1 could be used in further applications for the investigation of biological functions and pathological roles of H2S in living systems.
Department of Chemistry and Nano Science, Ewha Womans University , Seoul 120-750, Korea.
Yen Leng
YL
Pak
Department of Chemistry and Nano Science, Ewha Womans University , Seoul 120-750, Korea.
Jun
J
Li
Department of Chemistry, Sungkyunkwan University , Suwon 16419, Korea.
Kyoung Chul
KC
Ko
Department of Chemistry and Nano Science, Ewha Womans University , Seoul 120-750, Korea.
Gyoungmi
G
Kim
Department of Chemistry, Sungkyunkwan University , Suwon 16419, Korea.
Jin Yong
JY
Lee
Department of Chemistry and Nano Science, Ewha Womans University , Seoul 120-750, Korea.
Juyoung
J
Yoon
D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D053644:Naphthalimides / Q000737:chemistry / Q000633:toxicity; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D053644:Naphthalimides; D006862:Hydrogen Sulfide