Gramella sediminilitoris sp. nov., isolated from a tidal flat.
A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and coccoid, ovoid or rod-shaped bacterial strain, designated GHTF-27T, was isolated from a tidal flat at Goheung, South Korea, and subjected to a taxonomic study using a polyphasic approach. The novel strain grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-5.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GHTF-27Tbelongs to the genus Gramella. It exhibited 16S rRNA gene sequence similarity values of 97.6-98.3 % to the type strains of Gramella aestuariivivens, Gramella echinicola, Gramella gaetbulicola, Gramella aquimixticola, Gramella aestuarii and Gramella portivictoriae, respectively, and of 93.8-96.7 % to the type strains of the other species of the genus Gramellawith validly published names. Strain GHTF-27Tcontained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids of strain GHTF-27Twere phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain GHTF-27Twas 39.7 mol% and DNA-DNA relatedness values with the type strains of the six phylogenetically closely related species of the genus Gramellawere 12-28 %. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHTF-27Tis separated from other species of the genus Gramella. On the basis of the data presented, strain GHTF-27Tis considered to represent a novel species of the genus Gramella, for which the name Gramella sediminilitorissp. nov. is proposed. The type strain is GHTF-27T(=KCTC 52208T=NBRC 111992T).
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu Suwon, South Korea.
Sooyeon
S
Park
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu Suwon, South Korea.
Sun Young
SY
Yoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
Yong-Taek
YT
Jung
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu Suwon, South Korea.
Sung-Min
SM
Won
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu Suwon, South Korea.
Jung-Hoon
JH
Yoon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6
Gramella sediminilitoris sp. nov.; novel species; polyphasic taxonomy; tidal flat