Effect of methyl butyrate aroma on the survival and viability of human breast cancer cells in vitro.
BACKGROUND
Aroma can have far reaching effects on mind, body and soul. Pleasant aromas are known to have a soothing effect on the mind and are known to relieve stress and enhance concentration. Recently, it has been demonstrated that aroma may also have some curative effects as well as benefits and can be used both for prophylaxis and therapy of diseases. Our aim was to test our hypothesis whether aroma can cure or prevent cancer. Methyl butyrate (MB) is the methyl ester of butyric acid having a characteristic sweet and fruity odor like that of apples and pineapples. It occurs in many plant products in minute quantities and in pineapple oil.


METHODS
In the present study, the effect of aroma of MB has been evaluated on human breast cancer cell line MDA-MB-231 in vitro. The percentage viability of the cell line was determined by using Trypan blue dye exclusion assay.


RESULTS
It was found that MB at a concentration of 0.01M was effective in causing considerable cytotoxicity (40%) in breast cancer cells (without even coming in contact with cells) while at 0.02M, % cytotoxicity was found to be 50%. Mechanism of action of MB on cancer cells was investigated by acridine orange-ethidium bromide assay using fluorescence microscopy and DNA fragmentation assay. MB aroma appeared to induce necrosis in cancer cells exposed to it.


CONCLUSIONS
No study involving the effect of aroma/smell on cancer cells has ever been reported before and warrants further investigation on other cancer and normal cell lines.
Era's Lucknow Medical College & Hospital, Sarfarazganj, Hardoi Road, Lucknow-226003, India.
Mohsin Ali
MA
Khan
Dept. of Biochemistry, Era's Lucknow Medical College & Hospital, Sarfarazganj, Hardoi Road, Lucknow-226003, India. Electronic address: rumana_ahmad@yahoo.co.in.
Rumana
R
Ahmad
Dept. of Pathology, Era's Lucknow Medical College & Hospital, Sarfarazganj, Hardoi Road, Lucknow-226003, India.
A N
AN
Srivastava
D031230:Ananas / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects*; D019341:Aromatherapy; D001943:Breast Neoplasms / Q000628:therapy*; D002087:Butyrates / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D005260:Female; D006801:Humans; D027845:Malus / Q000737:chemistry
D002087:Butyrates; C043811:methyl butyrate
Breast cancer; Cytotoxicity; In-vitro; MDA-MB-231; Methyl butyrate