Minimally invasive screening for colitis using attenuated total internal reflectance fourier transform infrared spectroscopy.
This article describes a rapid, simple and cost-effective technique that could lead to a screening method for colitis without the need for biopsies or in vivo measurements. This screening technique includes the testing of serum using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy for the colitis-induced increased presence of mannose. Chronic (Interleukin 10 knockout) and acute (Dextran Sodium Sulphate-induced) models for colitis are tested using the ATR-FTIR technique. Arthritis (Collagen Antibody Induced Arthritis) and metabolic syndrome (Toll like receptor 5 knockout) models are also tested as controls. The marker identified as mannose uniquely screens and distinguishes the colitic from the non-colitic samples and the controls. The reference or the baseline spectrum could be the pooled and averaged spectra of non-colitic samples or the subject's previous sample spectrum. This shows the potential of having individualized route maps of disease status, leading to personalized diagnosis and drug management.
Department of Physics and Astronomy, GSU, Atlanta, GA 30303, USA.
Jitto
J
Titus
Institute for Biomedical Sciences, GSU, Atlanta, GA 30302, USA.|Center for Diagnostics and Therapeutics, GSU, Atlanta, GA 30302, USA.
Emilie
E
Viennois
Institute for Biomedical Sciences, GSU, Atlanta, GA 30302, USA.|Center for Diagnostics and Therapeutics, GSU, Atlanta, GA 30302, USA.|Atlanta Veterans Affairs Medical Center, Decatur, GA 30033, USA.
Didier
D
Merlin
Department of Physics and Astronomy, GSU, Atlanta, GA 30303, USA.|Center for Diagnostics and Therapeutics, GSU, Atlanta, GA 30302, USA.
A G
AG
Unil Perera
D000818:Animals; D001169:Arthritis, Experimental / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D001774:Blood Chemical Analysis; D003092:Colitis / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000378:metabolism / Q000473:pathology; D005243:Feces / Q000737:chemistry; D005260:Female; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D000071068:Lipocalin-2 / Q000378:metabolism; D024821:Metabolic Syndrome / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D051198:Toll-Like Receptor 5 / Q000235:genetics / Q000378:metabolism
C556062:IL10 protein, mouse; D000071068:Lipocalin-2; D051198:Toll-Like Receptor 5; C071332:Lcn2 protein, mouse; D016753:Interleukin-10
ATR-FTIR; colitis; minimally invasive; screening; sera