DNA-free genome editing methods for targeted crop improvement.
Evolution of the next-generation clustered, regularly interspaced, short palindromic repeat/Cas9 (CRISPR/Cas9) genome editing tools, ribonucleoprotein (RNA)-guided endonuclease (RGEN) RNPs, is paving the way for developing DNA-free genetically edited crop plants. In this review, I discuss the various methods of RGEN RNPs tool delivery into plant cells and their limitations to adopt this technology to numerous crop plants. Furthermore, focus is given on the importance of developing DNA-free genome edited crop plants, including perennial crop plants. The possible regulation on the DNA-free, next-generation genome-edited crop plants is also highlighted.
Research and Innovation Centre, Genomics and Biology of Fruit Crop Department, Fondazione Edmund Mach (FEM), Via Mach 1, 38010, San Michele all'Adige, TN, Italy. chidananda.nagamangala@fmach.it.
Chidananda Nagamangala
CN
Kanchiswamy
D064113:CRISPR-Cas Systems; D019295:Computational Biology / Q000379:methods; D018556:Crops, Agricultural / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D018745:Genome, Plant / Q000235:genetics*; D020407:Internet; D008957:Models, Genetic; D030821:Plants, Genetically Modified
CRISPR/Cas9; Genome editing; RGEN; RGEN RNPs