Antimicrobial activity and substantivity of Uncaria tomentosa in infected root canal dentin.
The aim of this study was to analyze the antimicrobial activity and substantivity of Uncaria tomentosa Willd DC (cat's claw, CC) in root dentin contaminated with Enterococcus faecalis. Forty-eight human premolars were contaminated with E. faecalis (ATCC 29212) and randomly divided into four groups according to the irrigant used during chemomechanical preparation (CMP): CC group: 2% CC gel; CHX group: 2% chlorhexidine digluconate gel (CHX); NaOCl group: 5.25% sodium hypochlorite (NaOCl); and SS group: sterile saline (SS). Microbiological samples were collected before (S1) and after (S2) CMP and after 7 days (S3). Colony-forming units (CFU/mL) at the different sampling times and comparisons among the groups were statistically analyzed by Wilcoxon and Kruskal-Wallis tests (p < 0.05). Significant bacterial reduction was achieved in all groups after CMP (p < 0.05). Results show no significant difference between S3 and S2 (p > 0.05) in the CC and CHX groups. Bacterial load was higher in S3 than in S2 samples (p < 0.05) in the NaOCl and SS groups. Our results suggest antibacterial effect of 2% CC gel against E. faecalis in infected dentin, in addition to antibacterial substantivity of 2% CC and 2% CHX up to 7 days.
Endodontic Division, Department of Restorative Dentistry, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
Daniel Rodrigo
DR
Herrera
Endodontic Division, Department of Restorative Dentistry, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
Jorge Enrique
JE
Durand-Ramirez
Endodontic Division, Department of Restorative Dentistry, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
Amanda
A
Falcão
Department of Endodontics, Health and Science Center, Universidade do Grande Rio, Rio de Janeiro, RJ, Brazil.
Emmanuel João Leal Nogueira da
EJ
Silva
Department of Dentistry, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
Elizabete Brasil Dos
EB
Santos
Endodontic Division, Department of Restorative Dentistry, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
Brenda Paula Figueiredo de Almeida
BP
Gomes
D000890:Anti-Infective Agents / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D020948:Cat's Claw / Q000737:chemistry*; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000187:drug effects* / Q000382:microbiology*; D003804:Dentin / Q000187:drug effects* / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D011897:Random Allocation; D012016:Reference Values; D012388:Root Canal Irrigants / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric; D013997:Time Factors
D000890:Anti-Infective Agents; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D002710:Chlorhexidine