Vcx1-D1 (M383I), the Vcx1 mutant with a calcineurin-independent vacuolar Ca(2+)/H(+) exchanger activity, confers calcineurin-independent Mn(2+) tolerance in Saccharomyces cerevisiae.
The Vcx1-M1 mutant is known to confer calcineurin-dependent Mn(2+) tolerance in budding yeast. Here, we demonstrate that another Vcx1 mutant, Vcx1-D1 with calcineurin-independent vacuolar Ca(2+)/H(+) exchanger activity, confers calcineurin-independent Mn(2+) tolerance. Unlike Vcx1-M1, the Mn(2+) tolerance conferred by Vcx1-D1 is dependent on the presence of Pmr1 or Pmc1. The Pmr1-dependent Mn(2+) tolerance of Vcx1-D1 requires the presence of calcineurin but not the functioning of the Ca(2+)/calcineurin signaling pathway. Similar to the wild-type Vcx1, C-terminally green fluorescent protein tagged Vcx1-D1 and Vcx1-M1 mutants localize to the endoplasmic reticulum instead of its normal vacuolar destination, but they remain functional in Ca(2+) sensitivity and Mn(2+) tolerance.
a The State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.|b The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
Yunying
Y
Zhao
b The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
Huihui
H
Xu
b The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
Yan
Y
Zhang
a The State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.|b The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
Linghuo
L
Jiang
D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008345:Manganese / Q000633:toxicity; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014617:Vacuoles / Q000378:metabolism
D017920:Antiporters; D029701:Saccharomyces cerevisiae Proteins; C099946:VCX1 protein, S cerevisiae; D008345:Manganese; D019703:Calcineurin; D000252:Calcium-Transporting ATPases; D002118:Calcium
VCX1; VCX1-D1; VCX1-M1; calcineurin; calcineurine; calcium; manganese resistance; résistance au manganese