Detection for cross-reactive proteins in filarial worm Setaria equina, MCF-7 human breast cancer, and Huh-7 hepatoma cells.
This study aimed to detect the cross-reactive proteins in filarial parasite adult worm Setaria equina and two different tumor cell lines (MCF-7 human breast cancer and Huh-7 hepatoma cells). This was performed using rabbit anti-S. equina extract (SeqE) or DEC (Diethylcarbamazine citrate) polyclonal IgG antibodies by indirect ELISA and western blotting. The results indicated cross-reactive bands at 70 and 75 kDa in all extracts by anti-DEC and SeqE antibodies, respectively. In addition, the expression of 70 kDa protein was only reduced in filarial worms and Huh-7 after in vitro DEC treatment compared to the control.
a Immunology Division, Zoology Department , Beni-Suef University , Beni-Suef , Egypt.
Mahmoud
M
Abdel-Latif
b Parasitology Division, Zoology Department , Beni-Suef University , Beni-Suef , Egypt.
Thabet
T
Sakran
D000818:Animals; D000941:Antigens / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D061986:MCF-7 Cells; D008297:Male; D011817:Rabbits; D017182:Setaria Nematode / Q000737:chemistry* / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000941:Antigens; D000951:Antigens, Neoplasm; D007074:Immunoglobulin G
cross-reaction; diethylcarbamazine citrate; filarial parasite; tumor cell line; western blotting