Single antigen flow beads for identification of human leukocyte antigen antibody specificities in hypersensitized patients with chronic renal failure.
OBJECTIVE
Aims of this study were to identify class I and class II antibodies in highly sensitized patients by flow cytometry single antigen bead (FC-SAB) assay and to evaluate according to donor HLA type in order to increase their kidney transplantation chance.


METHODS
We analyzed 60 hypersensitive patients of 351 individuals, who applied to our laboratory for PRA test in November 2013-December 2014. Flow cytometric PRA screening and single antigen bead commercial kits were used for these analyses.


RESULTS
In our study group, 19 (31.7%) of these patients were male while 41 (68.3%) patients were female. The most common acceptable antigens were A*02 (10.11%), HLA-A*23 (10.11%), HLA-B*38 (8.79%) and HLA-DRB1*03 (7.83%) in hypersensitive patients. The highest antibody reactivity on SAB was observed against HLA-A*25, HLA-B*45, HLA-DRB1*04 and HLA-DRB1*08 antigens.


CONCLUSIONS
The determination of these acceptable and unacceptable antigens may increase their transplantation chance. Pre-transplant HLA antibody identifications provide prognostic information with respect to the determination of patients who are at increased risk of graft loss.
Department of Medical Biology and Genetics, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey; Tissue Typing Laboratory, Izmir Tepecik Education and Research Hospital, İzmir, Turkey.
Mustafa
M
Soyöz
Department of Medical Biology and Genetics, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey; Tissue Typing Laboratory, Izmir Tepecik Education and Research Hospital, İzmir, Turkey.
Tülay
T
Kılıçaslan-Ayna
Department of Medical Biology and Genetics, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey; Tissue Typing Laboratory, Izmir Tepecik Education and Research Hospital, İzmir, Turkey.
Aslı
A
Özkızılcık-Koçyiğit
Tissue Typing Laboratory, Izmir Tepecik Education and Research Hospital, İzmir, Turkey.
Derya
D
Güleç
Department of Medical Biology and Genetics, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey; Tissue Typing Laboratory, Izmir Tepecik Education and Research Hospital, İzmir, Turkey.
İbrahim
İ
Pirim
DSA; HLA; flow cytometry; single antigen bead