The utility of azan trichrome staining in Ameloblastoma.
It is occasionally difficult to distinguish the stellate reticulum-like region of ameloblastoma from the fibrous connective tissue stroma. This difficulty is further pronounced in the plexiform variant of ameloblastoma that has very sparse fibrous connective tissue.



To test the utility of Azan trichrome stain in marking tumour regions and the peri-tumour environment of ameloblastoma.



Sections were prepared for 18 formalin fixed paraffin-embedded blocks of ameloblastoma cases and stained with Azan trichrome stain according to the manufacturer's specification.



The tumour areas were stained mostly brown, with the ameloblasts mainly marked as deep brown while the stellate reticulum-like region was light brown. The structures in the peri-tumour region were marked with different shades of blue. Azan trichrome staining was able to distinguish between the fibrous connective tissue and the stellate reticulum-like areas in 100% of the cases.
Akinyele O
AO
Adisa
Samuel E
SE
Udeabor
Alica
A
Kubesch
Mike
M
Barbeck
Department of Oral, Cranio Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main; REPAIR Lab, Institute of Pathology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
Shahram
S
Ghanaati
D000564:Ameloblastoma / Q000175:diagnosis*; D000818:Animals; D006801:Humans; D009549:Nigeria; D013194:Staining and Labeling