Direct reconstruction in CT-analogous pharmacokinetic diffuse fluorescence tomography: two-dimensional simulative and experimental validations.
We present a generalized strategy for direct reconstruction in pharmacokinetic diffuse fluorescence tomography (DFT) with CT-analogous scanning mode, which can accomplish one-step reconstruction of the indocyanine-green pharmacokinetic-rate images within in vivo small animals by incorporating the compartmental kinetic model into an adaptive extended Kalman filtering scheme and using an instantaneous sampling dataset. This scheme, compared with the established indirect and direct methods, eliminates the interim error of the DFT inversion and relaxes the expensive requirement of the instrument for obtaining highly time-resolved date-sets of complete 360 deg projections. The scheme is validated by two-dimensional simulations for the two-compartment model and pilot phantom experiments for the one-compartment model, suggesting that the proposed method can estimate the compartmental concentrations and the pharmacokinetic-rates simultaneously with a fair quantitative and localization accuracy, and is well suitable for cost-effective and dense-sampling instrumentation based on the highly-sensitive photon counting technique.
Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin 300072, China.
Xin
X
Wang
Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin 300072, China.
Yanqi
Y
Zhang
Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin 300072, ChinabTianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin 300072, China.
Limin
L
Zhang
Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin 300072, ChinabTianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin 300072, China.
Jiao
J
Li
Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin 300072, ChinabTianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin 300072, China.
Zhongxing
Z
Zhou
Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin 300072, ChinabTianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin 300072, China.
Huijuan
H
Zhao
Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin 300072, ChinabTianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin 300072, China.
Feng
F
Gao
D000465:Algorithms; D003198:Computer Simulation; D007208:Indocyanine Green / Q000737:chemistry; D008956:Models, Chemical; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000295:instrumentation / Q000379:methods*
D007208:Indocyanine Green