Two distinct pathways lead Bacillus thuringiensis to commit to sporulation in biofilm.
The spore-forming bacterium Bacillus thuringiensis is an efficient biofilm producer, responsible for persistent contamination of industrial food processing systems. B. thuringiensis biofilms are highly heterogeneous bacterial structures in which three distinct cell types controlled by quorum sensing regulators were identified: PlcR-controlled virulent cells, NprR-dependent necrotrophic cells and cells committed to sporulation, a differentiation process controlled by Rap phosphatases and Spo0A-P. Interestingly, a cell lineage study revealed that, in LB medium or in insect larvae, only necrotrophic cells became spores. Here we analyzed cellular differentiation undertaken by cells growing in biofilm in a medium optimized for sporulation. No virulent cells were identified; surprisingly, two distinct routes could lead to differentiation as a spore in this growth condition: the NprR-dependent route, followed by the majority of cells, and the newly identified NprR-independent route, which is followed by 20% of sporulating cells.
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Electronic address: verplaetse@gmail.com.
Emilie
E
Verplaetse
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Electronic address: leyla.slamti@jouy.inra.fr.
Leyla
L
Slamti
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Electronic address: michel.gohar@jouy.inra.fr.
Michel
M
Gohar
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Electronic address: Didier.Lereclus@jouy.inra.fr.
Didier
D
Lereclus
D001413:Bacillus thuringiensis / Q000254:growth & development* / Q000378:metabolism* / Q000472:pathogenicity; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D053038:Quorum Sensing / Q000502:physiology; D013171:Spores, Bacterial / Q000254:growth & development*; D014157:Transcription Factors / Q000378:metabolism
D014157:Transcription Factors
Bacillus cereus; Cell lineage; Necrotrophism; Quorum sensing; Transcriptional regulator; Virulence