Expression of Migration-Related Genes in Human Colorectal Cancer and Activity of a Disintegrin and Metalloproteinase 17.
BACKGROUND
The ability to form metastases which depends on the mechanisms of cell migration is an important element of the progression of cancer. In the present study we analyzed the genes involved in the regulation of migration in colon cancer cells.


METHODS
A total of 20 pairs of surgically removed tumoral and healthy (marginal) tissues samples from colorectal cancer patients at clinical stages I-II and III-IV were analyzed. The isolation of RNA from CRC and normal tissues and its subsequent molecular analysis were performed according to manufacturer's instructions. Microarray data analysis was performed using the GeneSpring 11.5 platform and Significance Analysis of Microarrays (SAM). In SAM analysis to identify significantly differentially expressed genes score and q-value parameters were used.


RESULTS
The largest increase in expression of genes was shown by MMP9, ADAM17, EphA2, and TIMP.


CONCLUSIONS
Presented genes, especially ADAM17, MMP9, EphA2, TIMP1, ICAM 11, and CD4, may be used as prognostic markers of advanced stages of colorectal cancer, contributing to the development of new lines of therapy focused on reducing metastasis of the primary tumor.
Department of Internal Medicine, Faculty of Public Health in Bytom, Medical University of Silesia, 40-055 Katowice, Poland.
Katarzyna
K
Walkiewicz
Department of Internal Medicine, Faculty of Public Health in Bytom, Medical University of Silesia, 40-055 Katowice, Poland.
Paweł
P
Kozieł
Department of Internal Medicine, Faculty of Public Health in Bytom, Medical University of Silesia, 40-055 Katowice, Poland.
Martyna
M
Bednarczyk
Department of Internal Medicine, Faculty of Public Health in Bytom, Medical University of Silesia, 40-055 Katowice, Poland.
Adam
A
Błażelonis
Department of Molecular Biology, Faculty of Pharmacy in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland.
Urszula
U
Mazurek
Department of Internal Medicine, Faculty of Public Health in Bytom, Medical University of Silesia, 40-055 Katowice, Poland.
Małgorzata
M
Muc-Wierzgoń
D000072198:ADAM17 Protein / Q000096:biosynthesis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002465:Cell Movement / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D046228:Microarray Analysis; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D036104:Receptor, EphA2 / Q000096:biosynthesis / Q000235:genetics; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis / Q000235:genetics
D014408:Biomarkers, Tumor; D019714:Tissue Inhibitor of Metalloproteinases; D036104:Receptor, EphA2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human