DNA-binding, cytotoxicity, cellular uptake, apoptosis and photocleavage studies of Ru(II) complexes.
Two Ru(II) complexes [Ru(phen)2bppp](ClO4)2 (1) and [Ru(phen)27-Br-dppz](ClO4)2 (2) [phen=1,10 phenanthroline, 7-Br-dppz=7-fluorodipyrido[3,2-a:2',3'-c]phenazine, bppp=11-bromo-pyrido[2',3':5,6]pyrazino[2,3-f] [1,10]phenanthroline] have been synthesized and characterized by elemental analysis, ES-MS, (1)H-NMR, (13)C-NMR and IR. The in vitro cytotoxicity of the complexes examined against a panel of cancer cell lines (HeLa, Du145 and A549) by MTT method, both complexes show prominent anticancer activity against various cancer cells. Live cell imaging study and flow cytometric analysis demonstrate that both the complexes 1 and 2 could cross the cell membrane accumulating in the nucleus. Further, flow cytometry experiments showed that the cytotoxic Ru(II) complexes 1 and 2 induced apoptosis of HeLa tumor cell lines. Photo induced DNA cleavage studies have been performed and results indicate that both the complexes efficiently photo cleave pBR322 DNA. The binding properties of two complexes toward CT-DNA were investigated by various optical methods and viscosity measurements. The experimental results suggested that both Ru(II) complexes can intercalate into DNA base pairs. The complexes were docked into DNA-base pairs using the GOLD docking program.
Department of Chemistry, Osmania University, Hyderabad, Telangana 500007, India.
N Deepika
Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan, ROC.
C Shobha Devi
Department of Chemistry, Osmania University, Hyderabad, Telangana 500007, India.
Y Praveen Kumar
Department of Chemistry, Osmania University, Hyderabad, Telangana 500007, India.
K Laxma Reddy
Department of Chemistry, Osmania University, Hyderabad, Telangana 500007, India.
P Venkat Reddy
Department of Biochemistry, Osmania University, Hyderabad, Telangana 500007, India.
D Anil Kumar
Department of Biochemistry, Osmania University, Hyderabad, Telangana 500007, India.
Surya S Singh
Department of Chemistry, Osmania University, Hyderabad, Telangana 500007, India.
S Satyanarayana
D000890:Anti-Infective Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D055668:Photochemical Processes; D017975:Ruthenium Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
D000890:Anti-Infective Agents; D017975:Ruthenium Compounds; D004247:DNA
Apoptosis; Cytotoxicity; DNA binding; Docking; Photocleavage; Ruthenium(II) polypyridyl complexes