Primary pulmonary clear cell sarcoma-the first two reported cases.
We report two cases (male patients 50 and 55 years old) of clear cell sarcoma ("melanoma of soft parts") arising in the lung, of which one case showed regional lymph node metastases. Histologically, both tumors displayed varying clear epithelioid and spindle neoplastic cells arranged in storiform and nested growth patterns, separated by thin fibrovascular septa. Immunohistochemical studies demonstrated positive expression of S-100 protein, HMB-45 and Melan-A in one case and S-100 protein only in the other. Fluorescence in situ hybridization showed positive EWSR1 gene rearrangement, and a presence of EWS-ATF1 fusion transcript was confirmed by RT-PCR and sequencing in one case.
Department of Pathology, National University Health System, 5 Lower Kent Ridge Road, 119074, Singapore.
Giap Hean
GH
Goh
Department of Pathology, National University Health System, 5 Lower Kent Ridge Road, 119074, Singapore.
Ming
M
Teh
Department of Molecular Genetics, Bioptical Laboratory Ltd, Pilsen, Czech Republic.
Tomas
T
Vanecek
Department of Pathology, University of Texas, MD Anderson Cancer Centre, Houston, Texas, USA.
Cesar
C
Moran
Department of Pathology, National University Health System, 5 Lower Kent Ridge Road, 119074, Singapore. fredrikpetersson@live.se.
Fredrik
F
Petersson
D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015321:Gene Rearrangement / Q000502:physiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008875:Middle Aged; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D018227:Sarcoma, Clear Cell / Q000175:diagnosis / Q000235:genetics*; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics*
D002148:Calmodulin-Binding Proteins; C500986:EWSR1 protein, human; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins; D009418:S100 Proteins
ATF1; Clear cell sarcoma; EWS; Lung; Melanoma of soft parts