[Difference of rhizosphere microbe quantity and functional diversity among three flue-cured tobacco cultivars with different resistance].
Field experiments were conducted in Shilin and Xundian respectively to study the diffe- rence of rhizosphere microbe quantity and functional diversity with plate culture method and Biolog technique among Hongda (high susceptibility, S), Yun87 (middle resistance, MR) and K326 (high resistance, R) , three flue-cured tobacco cultivars with different resistance to bacterial wilt and black shank. The results indicated that the amounts of bacteria, actinomycetes and the total number of microbes in tobacco plants' rhizosphere were positively correlated with the cultivar' s re- sistance, while it was opposite for the fungi. The consistent tendency was obtained not only at 35 d, 55 d and 75 d after transplanting, but also at two experimental sites. Cultivar and experimental con- ditions greatly affected the utilization of six types of carbon source by rhizospheric microbes, as well as the AWCD value. In Xundian site, rhizospheric microbes' utilization of carbohydrates, amino acids, carboxylic acids, polymers,. amines and the AWCD value were all higher at 55 d and 75 d after transplanting for the resistant cultivar than the susceptible one, but it was opposite at 75 d after transplanting for the phenolic acids. In Shilin, significant differences existed among the three culti- vars at 35 d, 55 d and 75 d after transplanting for the indices mentioned above, although they were not consistent with cultivars' resistance. Principal component (PC) analysis even showed that utili- zation of carbon sources by rhizosphere microorganisms differed significantly among the three culti- vars at the two sites, and it was better reflected by PC1 and PC2 at 55 d and 75 d after transplan- ting, respectively. In conclusion, rhizosphere microbial community structure and functional diversity were greatly affected not only by the cultivars' difference in resistance, but also by experimental conditions.
Qiu-hua
QH
Cai
Jin-xiang
JX
Zuo
Zhong-huan
ZH
Li
Ya-ping
YP
Zhang
Yong-gang
YG
Zhao
Qiao
Q
Deng
Jin
J
Ouyang
Jun-jie
JJ
Huang
Lu
L
Yu
Jian
J
Zou
Zheng-xiong
ZX
Zhao
D039903:Actinobacteria / Q000145:classification; D000596:Amino Acids / Q000378:metabolism; D044822:Biodiversity; D002244:Carbon / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D060467:Disease Resistance; D062385:Hydroxybenzoates / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014026:Nicotiana / Q000382:microbiology*
D000596:Amino Acids; D002264:Carboxylic Acids; D062385:Hydroxybenzoates; D012987:Soil; D002244:Carbon; C017616:phenolic acid