The nonylphenol biodegradation study by estuary sediment-derived fungus Penicillium simplicissimum.
Nonylphenols (NPs) are persistent organic pollutants (POPs) with estrogenic properties that can perform endocrine-disrupting activities. By using high-concentration NP as environmental selection pressure, one NP biodegradation strain named NPF-4 was isolated and purified from estuary sediment of the Moshui River. It was identified as Penicillium simplicissimum (PS1) by appearance and 18S rDNA analysis. In different culture situations, the strain mass growth and biodegradation ability were evaluated. Within 4-n-nonylphenol (4-n-NP) initial concentration of 20 mg L(-1), it could be degraded 53.76, 90.08, and 100.00 % at 3, 7, and 14 days, respectively. In feeding experiments, it showed that NPF-4 could use 4-n-NP as a sole carbon source. Based on seven products/intermediates detected with GC and LC-MS, a novel biopathway for 4-n-NP biodegradation was proposed, in which sequential hydroxylation, oxidation, and decarboxylation at terminal β-C atom may occur for 4-n-NP detoxification, even complete mineralization in the end.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao, 266100, Shandong, People's Republic of China.|College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, Shandong, People's Republic of China.
Yan
Y
Zhang
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, Shandong, People's Republic of China.|Qingdao Fishery Technology Service Station, Qingdao, 266071, Shandong, People's Republic of China.
Ying
Y
Liu
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, Shandong, People's Republic of China.
Han
H
Dong
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao, 266100, Shandong, People's Republic of China.|College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, Shandong, People's Republic of China.
Xianguo
X
Li
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao, 266100, Shandong, People's Republic of China. dahaizhang@ouc.edu.cn.|College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, Shandong, People's Republic of China. dahaizhang@ouc.edu.cn.
Dahai
D
Zhang
D001673:Biodegradation, Environmental; D002244:Carbon; D002853:Chromatography, Liquid; D046508:Culture Techniques; D063366:Estuaries; D019015:Geologic Sediments / Q000382:microbiology; D013058:Mass Spectrometry; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D045483:Rivers / Q000382:microbiology; D054712:Salinity
D010636:Phenols; D002244:Carbon; C025256:nonylphenol; C041594:4-nonylphenol
Biodegradation; Biopathway; Fungus; Metabolite; Nonylphenol; Penicillium simplicissimum