Quantum dots-hemin: Preparation and application in the absorption of heme iron.
The absorption mechanism of heme iron remains unclear due to the limit of labeling techniques. Quantum dots (QDs) are powerful fluorescent probes resistant to photobleaching, however, there is no data about the application of QDs in heme iron absorption. Herein, we prepared hemin-coated CdSe/ZnS (QDs-hemin), and studied their absorption in vitro and in vivo. Results showed that QDs-hemin had uniform particle sizes, physiological stability and high joint efficiency. Moreover, QDs-hemin could be successfully absorbed gradually into the duodenum with the time using synchrotron radiation micro X-ray fluorescence and confocal laser scanning microscopy. Furthermore, QDs-hemin were observed to degrade in lysosomes, and their absorption was blocked by Heme Carrier Protein 1 (HCP1) antibody and HCP1 siRNA. All the results demonstrate that QDs can be a good tracer for heme iron and that HCP1 pathway is critical and predominant over the endocytosis pathway in the absorption mechanism.
College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, China.
Lina
L
Geng
Key Laboratory of Animal Physiology, Biochemistry & Molecular Biology of Hebei Province, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Xianglin
X
Duan
Key Laboratory of Animal Physiology, Biochemistry & Molecular Biology of Hebei Province, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Yan
Y
Wang
Key Laboratory of Animal Physiology, Biochemistry & Molecular Biology of Hebei Province, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Yashuo
Y
Zhao
Key Laboratory of Animal Physiology, Biochemistry & Molecular Biology of Hebei Province, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China.
Guofen
G
Gao
College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, China.
Delong
D
Liu
Key Laboratory of Animal Physiology, Biochemistry & Molecular Biology of Hebei Province, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China. Electronic address: chang7676@163.com.
Yan-Zhong
YZ
Chang
Key Laboratory of Animal Physiology, Biochemistry & Molecular Biology of Hebei Province, Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang, China. Electronic address: yupeng0311@mail.hebtu.edu.cn.
Peng
P
Yu
D000818:Animals; D004386:Duodenum; D005456:Fluorescent Dyes; D006427:Hemin / Q000493:pharmacokinetics*; D007501:Iron; D051379:Mice; D010316:Particle Size; D011484:Protein-Arginine N-Methyltransferases; D045663:Quantum Dots
D005456:Fluorescent Dyes; D006427:Hemin; D007501:Iron; D011484:Protein-Arginine N-Methyltransferases
Heme; Heme carrier protein 1; Intestine; Micro X-ray fluorescence; Quantum dots