Frizzled-4 C-terminus Distal to KTXXXW Motif is Essential for Normal Dishevelled Recruitment and Norrin-stimulated Activation of Lef/Tcf-dependent Transcriptional Activation.
The carboxy (C)-termini of G protein coupled receptors (GPCR) dictate essential functions. The KTXXXW motif C-terminus of Frizzleds (FZD) has been implicated in recruitment of Dishevelled (DVL). Through study of FZD4 and its associated ligand Norrin, we report that a minimum of three residues distal to the KTXXXW motif in the C-terminal tail of Frizzled-4 are essential for DVL recruitment and robust Lef/Tcf-dependent transcriptional activation in response to Norrin.
Department of Physiology & Biophysics, Health Sciences Center, School of Medicine, Stony Brook University, Stony Brook, NY 11794-8661, USA.
Alexander C
AC
Bertalovitz
Department of Physiology & Biophysics, Health Sciences Center, School of Medicine, Stony Brook University, Stony Brook, NY 11794-8661, USA.
Milly S
MS
Pau
Department of Pharmacology, Health Sciences Center, School of Medicine, Stony Brook University, Stony Brook, NY 11794-8651, USA.
Shujuan
S
Gao
Department of Pharmacology, Health Sciences Center, School of Medicine, Stony Brook University, Stony Brook, NY 11794-8651, USA.
Craig C
CC
Malbon
Department of Physiology & Biophysics, Health Sciences Center, School of Medicine, Stony Brook University, Stony Brook, NY 11794-8661, USA.
Hsien-Yu
HY
Wang
Dishevelled; Frizzled; Frizzled-4; Norrin; carboxy-terminus; helix VIII