Anti-osteoarthritic activity of Bungarus fasciatus venom fraction BF-F47 involving molecular markers in the rats.
A heat stable protein BF-F47 was purified from the crude venom of Bungarus fasciatus by CM cellulose ion exchange chromatography and HPLC. Osteoarthritis (OA) was developed in male albino Wistar rats by collagenase injection. BF-F47 treatment significantly restored urinary hydroxyproline and glucosamine in OA rats. Serum acid phosphatase, alkaline phosphatase, creatinine and serum molecular markers TNF-α, IL-1β, IL-17, cytokine induced neutrophil chemoattractant-1, matrix metalloproteinase-1, cathepsin-K, osteocalcin and PGE2 were also significantly altered. BF-F47 showed partial restoration of osteoarthritis joints. Thus, BF-F47 induced anti-osteoarthritic activity in Wistar rats acted through molecular markers of arthritis and inflammation.
Laboratory of Toxinology & Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, 92, A.P.C Road, Kolkata, 700009, India. Electronic address: agomescu@gmail.com.
Antony
A
Gomes
Laboratory of Toxinology & Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, 92, A.P.C Road, Kolkata, 700009, India.
Susmita
S
Ghosh
Laboratory of Toxinology & Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, 92, A.P.C Road, Kolkata, 700009, India.
Sourav
S
Ghosh
Laboratory of Toxinology & Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, 92, A.P.C Road, Kolkata, 700009, India.
Kalyani
K
Saha
Department of Zoology, J K College, Purulia, West Bengal, India.
Partha Pratim
PP
Saha
Post Graduate Department of Zoology, Maulana Azad College, Kolkata, West Bengal, India.
Subir Chandra
SC
Dasgupta
Former Chief Scientist, CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, India.
Aparna
A
Gomes
D000818:Animals; D001688:Biological Products / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism / Q000652:urine; D050071:Bone Density Conservation Agents / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D017838:Bungarus; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D015232:Dinoprostone / Q000097:blood; D004195:Disease Models, Animal; D004546:Elapid Venoms / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D005944:Glucosamine / Q000652:urine; D006909:Hydroxyproline / Q000652:urine; D007194:India; D018836:Inflammation Mediators / Q000097:blood; D007274:Injections, Intraperitoneal; D007596:Joints / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D010003:Osteoarthritis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D015675:Osteocalcin / Q000097:blood; D055550:Protein Stability; D017208:Rats, Wistar; D030162:Reptilian Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*
D001688:Biological Products; D015415:Biomarkers; D050071:Bone Density Conservation Agents; D004546:Elapid Venoms; D018836:Inflammation Mediators; D030162:Reptilian Proteins; D015675:Osteocalcin; D015232:Dinoprostone; D005944:Glucosamine; D006909:Hydroxyproline
Arthritis; Banded krait; Bungarus fasciatus; Inflammation; Osteoarthritis; Snake venom