Diversity of indigenous endophytic bacteria associated with the roots of Chinese cabbage (Brassica campestris L.) cultivars and their antagonism towards pathogens.
The study aimed to reveal the diversity of endophytic bacteria in the roots of Chinese cabbage (CC) cultivated in two areas in Korea, namely, Seosang-gun (SS) and Haenam-gun (HN), and also in a transgenic plant (TP) from the laboratory. A total of 653 colonies were isolated from the interior of CC roots, comprising 118, 302, and 233 isolates from SS, HN, and TP samples, respectively. Based on 16S rRNA gene sequence analysis, the isolates belonged to four major phylogenetic groups: high-G+C Gram-positive bacteria (HGC-GPB), low-G+C Gram-positive bacteria (LGC-GPB), Proteobacteria, and Bacteriodetes. The most dominant groups in the roots of the SS, HN, and TP cultivars were LGC-GPB (48.3%), Proteobacteria (50.2%), and HGC-GPB (38.2%), respectively. Importantly, most of the isolates that produced cell-walldegrading enzymes belonged to the genus Bacillus. Bacillus sp. (HNR03, TPR06), Bacillus pumilus (SSR07, HNR11, TPR07), and Bacillus subtilis (TPR03) showed high antagonism against the tested food-borne pathogenic bacteria. In addition, Bacillus sp. (HNR03, TPR06), Bacillus pumilus (SSR07, HNR11, HNR17, TPR11), Microbacterium oxidans (SSR09, TPR04), Bacillus cereus HNR10, Pseudomonas sp. HNR13, and Bacillus subtilis (TPR02, TPR03) showed strong antagonistic activity against the fungi Phythium ultimum, Phytophthora capsici, Fusarium oxysporum, and Rhizoctonia solani. The endophytes isolated from the TP cultivar showed the strongest antagonistic reactions against pathogens. This study is the first report on endophytic bacteria from Chinese cabbage roots.
Department of Food Science, Gyeongnam National University of Science and Technology, Jinju, 660-758, Republic of Korea.|Department of Biochemistry & Molecular Biology, Hajee Mohammad Danesh Science & Technology University, Dinajpur, 5200, Bangladesh.
Md Azizul
MA
Haque
Research Institute of Life Sciences, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Han Dae
HD
Yun
Department of Food Science, Gyeongnam National University of Science and Technology, Jinju, 660-758, Republic of Korea. kmcho@gntech.ac.kr.
Kye Man
KM
Cho
D000898:Antibiosis; D000935:Antifungal Agents / Q000494:pharmacology; D001419:Bacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D001937:Brassica / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000382:microbiology; D056910:Republic of Korea; D012988:Soil Microbiology
D000935:Antifungal Agents; D004269:DNA, Bacterial
Chinese cabbage root; antimicrobial activity; endophytic bacteria; phylogene