Receptor for advanced glycation end products (RAGE) knockout reduces fetal dysmorphogenesis in murine diabetic pregnancy.
The receptor for Advanced Glycation End products (RAGE) is implicated in the pathogenesis of diabetic complications, but its importance in diabetic embryopathy is unclear. We therefore investigated the role of RAGE in diabetic embryopathy using streptozotocin induced diabetes in female wild type (WT) C57Bl/6N and RAGE knockout C57Bl/6N (RAGE(-/-)) mice, mated with control males of the same genotype. Maternal diabetes induced more fetal resorption and malformation (facial skeleton, neural tube) in the WT than in the RAGE(-/-) fetuses. Maternal plasma glucose and methylgyoxal concentrations, as well as embryonic N(ε)-carboxymethyl-lysine (CML) levels were increased to the same extent in diabetic WT and RAGE(-/-) pregnancy. However, maternal diabetes induced increased fetal hepatic isoprostane 8-iso-PGF2α levels (oxidative stress marker) and embryonic activation of NFκB in WT only (not in RAGE(-/-) embryos). The association between RAGE knockout and diminished embryonic dysmorphogenesis in diabetic pregnancy suggests that embryonic RAGE activation is involved in diabetic embryopathy.
Department of Medical Cell Biology, Medical Faculty of Uppsala University, Biomedical Centre, P.O. Box 751, SE-75123 Uppsala, Sweden. Electronic address: andreas.ejdesjo@mcb.uu.se.
Andreas
A
Ejdesjö
Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, INF 410, Heidelberg and German Center for Diabetes Research (DZD), Heidelberg, Germany.
Sebastian
S
Brings
Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, INF 410, Heidelberg and German Center for Diabetes Research (DZD), Heidelberg, Germany.
Thomas
T
Fleming
Department of Medical Cell Biology, Medical Faculty of Uppsala University, Biomedical Centre, P.O. Box 751, SE-75123 Uppsala, Sweden.
Rikard G
RG
Fred
Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, INF 410, Heidelberg and German Center for Diabetes Research (DZD), Heidelberg, Germany.
Peter P
PP
Nawroth
Department of Medical Cell Biology, Medical Faculty of Uppsala University, Biomedical Centre, P.O. Box 751, SE-75123 Uppsala, Sweden.
Ulf J
UJ
Eriksson
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000235:genetics; D015237:Dinoprost / Q000031:analogs & derivatives / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D047109:Fetal Development; D005327:Fetal Resorption; D015870:Gene Expression; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000235:genetics; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000097:blood / Q000235:genetics; D011765:Pyruvaldehyde / Q000097:blood; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics*
D001786:Blood Glucose; D016328:NF-kappa B; D000067759:Receptor for Advanced Glycation End Products; C075750:8-epi-prostaglandin F2alpha; C048496:N(6)-carboxymethyllysine; D011765:Pyruvaldehyde; D015237:Dinoprost; D008239:Lysine
AGE; Diabetes; Diabetic embryopathy; Experimental teratology; Neural tube defects; Oxidative stress; RAGE