Conserved features of complexes of TATA-box binding proteins with DNA.
A comparative analysis of all available structures of complexes of TATA-box binding proteins (TBPs) with DNA is performed. Conserved features of DNA-protein interaction are described, including nine amino acid residues that form conserved hydrogen bonds, 13 residues participating in formation of two conserved hydrophobic clusters at DNA-protein interface, and four conserved water-mediated contacts. Partial symmetry of conserved contacts reflects quasi-symmetry of TBP structure.
* Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1 Leninskiye Gory, bld.40, Moscow 119991, Russia.
Olga
O
Zanegina
* Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1 Leninskiye Gory, bld.40, Moscow 119991, Russia.
Evgeniy
E
Aksianov
* Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1 Leninskiye Gory, bld.40, Moscow 119991, Russia.|† Institute of System Studies, 36 Nakhimovski Prospect Corp. 1, Moscow 117218, Russia.
Andrei V
AV
Alexeevski
* Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1 Leninskiye Gory, bld.40, Moscow 119991, Russia.|‡ Gamaleya Center of Epidemiology and Microbiology, 18 Gamaleya st., Moscow 123098, Russia.|§ Institute of Agricultural Biotechnology, 42 Timiryazevskaya st., Moscow 127422, Russia.
Anna
A
Karyagina
* Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1 Leninskiye Gory, bld.40, Moscow 119991, Russia.|† Institute of System Studies, 36 Nakhimovski Prospect Corp. 1, Moscow 117218, Russia.
Sergei
S
Spirin
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D011487:Protein Conformation; D035181:TATA-Box Binding Protein / Q000737:chemistry* / Q000378:metabolism*; D014867:Water
D035181:TATA-Box Binding Protein; D014867:Water; D004247:DNA
DNA–protein interaction; Structural bioinformatics; TATA-box binding protein