Actinomadura adrarensis sp. nov., an actinobacterium isolated from Saharan soil.
A novel actinobacterial strain, designated ACD12T, was isolated from a Saharan soil sample collected from Adrar province, southern Algeria. A polyphasic study was carried out to establish the taxonomic position of this strain. Strain ACD12Twas observed to form extensively branched substrate mycelia. Aerial mycelium was absent or was weakly produced on all media tested, while spore chains were short with a hooked and irregular spiral form (2-3 turns). The dominant diaminopimelic acid isomer in the cell wall was meso-diaminopimelic acid. Glucose, ribose, galactose, mannose and madurose occured in whole-cell hydrolysates. The major phospholipid was diphosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H6). The fatty acid profile was characterized by the presence of C16 : 0, C17 : 0, C15 : 0, C18 : 0, C18 : 1 cis9 and iso-C16 : 0. Results of 16S rRNA gene sequence comparisons revealed that strain ACD12Tshared the highest degree of 16S rRNA gene sequence similarity with Actinomadura sputi DSM 45233T(98.3 %) and Actinomadura hallensis DSM 45043T(97.8 %). All tree-making algorithms used also supported strain ACD12Tforming a distinct clade with its most closely related species. In addition, DNA-DNA hybridization indicated only 39.8 % relatedness with A. sputi DSM 45233Tand 18.7 % relatedness with A. hallensis DSM 45043T. The combined phenotypic and genotypic data show that the novel isolate represents a novel species of the genus Actinomadura, for which the name Actinomadura adrarensis sp. nov., is proposed, with the type strain ACD12T(=DSM 46745T=CECT 8842T).
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger, Algeria.
Abdelhadi
A
Lahoum
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger, Algeria.|Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa 47000, Algeria.
Noureddine
N
Bouras
Université de Toulouse, Laboratoire de Génie Chimique UMR 5503 (CNRS/INPT/UPS), INP de Toulouse/ENSAT, 1, avenue de l'Agrobiopôle, Castanet-Tolosan cedex, France.
Carol
C
Verheecke
Université de Toulouse, Laboratoire de Génie Chimique UMR 5503 (CNRS/INPT/UPS), INP de Toulouse/ENSAT, 1, avenue de l'Agrobiopôle, Castanet-Tolosan cedex, France.
Florence
F
Mathieu
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Peter
P
Schumann
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Cathrin
C
Spröer
School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
Hans-Peter
HP
Klenk
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger, Algeria.
Nasserdine
N
Sabaou
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000352:Africa, Northern; D000462:Algeria; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid
Actinomadura; Actinomadura adrarensis sp. nov.; Saharan soil; actinobacteria; bcterial diversity; polyphasic taxonomy