Arabidopsis mitochondrial protein slow embryo development1 is essential for embryo development.
The plant seeds formation are crucial parts in reproductive process in seed plants as well as food source for humans. Proper embryo development ensure viable seed formation. Here, we showed an Arabidopsis T-DNA insertion mutant slow embryo development1 (sed1) which exhibited retarded embryogenesis, led to aborted seeds. Embryo without SED1 developed slower compared to normal one and could be recognized at early globular stage by its white appearance. In later development stage, storage accumulated poorly with less protein and lipid body production. In vitro culture did not rescue albino embryo. SED1 encoded a protein targeted to mitochondria. Transmission electron microscopic analysis revealed that mitochondria developed abnormally, and more strikingly plastid failed to construct grana in time in sed1/sed1 embryo. These data indicated that SED1 is indispensable for embryogenesis in Arabidopsis, and the mitochondria may be involved in the regulation of many aspects of seed development.
Key Laboratory of Cell Proliferation and Differentiation (Ministry of Education), College of Life Sciences, Peking University, Beijing 100871, China.
Yan
Y
Ju
Key Laboratory of Cell Proliferation and Differentiation (Ministry of Education), College of Life Sciences, Peking University, Beijing 100871, China.
Chunying
C
Liu
Key Laboratory of Cell Proliferation and Differentiation (Ministry of Education), College of Life Sciences, Peking University, Beijing 100871, China.
Wenwen
W
Lu
Key Laboratory of Cell Proliferation and Differentiation (Ministry of Education), College of Life Sciences, Peking University, Beijing 100871, China.
Quan
Q
Zhang
Key Laboratory of Cell Proliferation and Differentiation (Ministry of Education), College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: sodmergn@pku.edu.cn.
Sodmergen
D017360:Arabidopsis / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D024101:Mitochondrial Proteins / Q000378:metabolism*; D012639:Seeds / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism*
D029681:Arabidopsis Proteins; D024101:Mitochondrial Proteins; C000618474:Sed1 protein, Arabidopsis
Arabidopsis thaliana; Embryo development; Mitochondrial protein; SED1