Expression Analysis of Ni- and V-Associated Resistance Genes in a Bacillus megaterium Strain Isolated from a Mining Site.
Bacillus megaterium strain MNSH1-9K-1 was isolated from a mining site in Guanajuato, Mexico. This B. megaterium strain presented the ability to remove Ni and V from a spent catalyst. Also, its associated metal resistance genes nccA, hant, VAN2, and smtAB were previously identified by a PCR approach. The present study reports for the first time, in B. megaterium, the changes in the expression of the genes nccA (Ni-Co-Cd resistance); hant (high-affinity nickel transporter); smtAB, a metal-binding protein gene; and VAN2 (V resistance) after exposure to 200 ppm of Ni and 200 ppm of V during the stationary phase of the microorganism in PHGII liquid media. The data presented here may contribute to the knowledge of the genes involved in the Ni and V resistances of B. megaterium, and the possible pathways implicated in the Ni-V removal processes, which may be potentiated for the biological treatment of high metal content residues.
Centro de Investigación de Ciencia Aplicada y Tecnología Avanzada, Instituto Politecnico Nacional, Querétaro, Mexico.
Grisel
G
Fierros Romero
Centro de Investigación de Ciencia Aplicada y Tecnología Avanzada, Instituto Politecnico Nacional, Querétaro, Mexico.
Andrea
A
Rivas Castillo
Centro de Investigación de Ciencia Aplicada y Tecnología Avanzada, Instituto Politecnico Nacional, Querétaro, Mexico.
Marlenne
M
Gómez Ramírez
Centro de Investigación de Ciencia Aplicada y Tecnología Avanzada, Instituto Politecnico Nacional, Querétaro, Mexico.
Reynaldo
R
Pless
Centro de Investigación de Ciencia Aplicada y Tecnología Avanzada, Instituto Politecnico Nacional, Querétaro, Mexico. nrojasa@ipn.mx.
Norma
N
Rojas Avelizapa
D001410:Bacillus megaterium / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D008800:Mexico; D008906:Mining; D009532:Nickel / Q000378:metabolism*; D012988:Soil Microbiology; D014639:Vanadium / Q000378:metabolism*
D001426:Bacterial Proteins; D014639:Vanadium; D009532:Nickel