Facile fabrication of FeN nanoparticles/nitrogen-doped graphene core-shell hybrid and its use as a platform for NADH detection in human blood serum.
Herein, we present a novel strategy for the synthesis of an iron nitride nanoparticles-encapsulated nitrogen-doped graphene (FeN NPs/NG) core-shell hierarchical nanostructure to boost the electrochemical performance in a highly sensitive, selective, reproducible, and stable sensing platform for nicotinamide adenine dinucleotide (NADH). This core-shell hierarchical nanostructure provides an excellent conductive network for effective charge transfer and avoids the agglomeration and restacking of NG sheets, which provides better access to the electrode material for NADH oxidation. The FeN NPs/NG core-shell hierarchical nanostructure demonstrates direct and mediatorless responses to NADH oxidation at a low potential. This material displays a high sensitivity of 0.028μA/μMcm(2), a wide linear range from 0.4 to 718μM, and a detection limit of 25nM with a fast response time of less than 3s. The interferences from common interferents, such as glucose, uric acid, dopamine, and ascorbic acid, are negligible. The fabricated sensor was further tested for the determination of NADH in human blood serum. The resulting high sensitivity, excellent selectivity, outstanding stability, and good reproducibility make the proposed FeN NPs/NG core-shell hierarchical nanostructure as a promising candidate for biomedical applications.
Advanced Materials Institute of BIN Convergence Technology (BK21 Plus Global) & Department of BIN Convergence Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
Jayaraman
J
Balamurugan
Advanced Materials Institute of BIN Convergence Technology (BK21 Plus Global) & Department of BIN Convergence Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
Tran Duy
TD
Thanh
Advanced Materials Institute of BIN Convergence Technology (BK21 Plus Global) & Department of BIN Convergence Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
Nam Hoon
NH
Kim
Advanced Materials Institute of BIN Convergence Technology (BK21 Plus Global) & Department of BIN Convergence Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea; Carbon Composite Research Centre, Department of Polymer - Nano Science and Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea. Electronic address: jhl@chonbuk.ac.kr.
Joong Hee
JH
Lee
D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007501:Iron / Q000737:chemistry*; D057230:Limit of Detection; D009243:NAD / Q000097:blood*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009584:Nitrogen / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results
D009243:NAD; D006108:Graphite; D007501:Iron; D009584:Nitrogen
Biosensors; Electrocatalysis; FeN nanoparticles; NADH; Nitrogen-doped graphene