Effect of bone marrow derived mesenchymal stem cells on lung pathology and inflammation in ovalbumin-induced asthma in mouse.
OBJECTIVE
Bone marrow-derived mesenchymal stem cells (BMSCs) have attracted significant interest to treat asthma and its complication. In this study, the effects of BMSCs on lung pathology and inflammation in an ovalbumin-induced asthma model in mouse were examined.


METHODS
BALB/c mice were divided into three groups: control group (animals were not sensitized), asthma group (animals were sensitized by ovalbumin), asthma+BMSC group (animals were sensitized by ovalbumin and treated with BMSCs). BMSCs were isolated and characterized and then labeled with Bromodeoxyuridine (BrdU). After that the cells transferred into asthmatic mice. Histopathological changes of the airways, BMSCs migration and total and differential white blood cell (WBC) count in bronchoalveolar lavage (BAL) fluid were evaluated.


RESULTS
A large number of BrdU-BMSCs were found in the lungs of mice treated with BMSCs. The histopathological changes, BAL total WBC counts and the percentage of neutrophils and eosinophils were increased in asthma group compared to the control group. Treatment with BMSCs significantly decreased airway pathological indices, inflammatory cell infiltration, and also goblet cell hyperplasia.


CONCLUSIONS
The results of this study revealed that BMSCs therapy significantly suppressed the lung pathology and inflammation in the ovalbumin induced asthma model in mouse.
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Maryam
M
Mohammadian
Neurogenic Inflammation Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad Hosein
MH
Boskabady
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iraj Ragerdi
IR
Kashani
Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Gila Pirzad
GP
Jahromi
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Amene
A
Omidi
Department of Emergency Medical Services, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Amir Kavian
AK
Nejad
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Safoura
S
Khamse
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Hamid Reza
HR
Sadeghipour
Inflammation; Lung pathology; Ovalbumin- induced asthma; Stem cells