Androgen receptor regulated microRNA miR-182-5p promotes prostate cancer progression by targeting the ARRDC3/ITGB4 pathway.
MicroRNAs (miRNAs) are important endogenous gene regulators that play key roles in prostate cancer development and metastasis. However, specific miRNA expression patterns in prostate cancer tissues from Chinese patients remain largely unknown. In this study, we compared miRNA expression patterns in 65 pairs of prostate cancer and para-cancer tissues by RNA sequencing and found that miR-182-5p was the most up-regulated miRNA in prostate cancer tissues. The result was validated using realtime PCR in 18 pairs of prostate cancer and para-cancer tissues. In in vitro analysis, it was confirmed that miR-182-5p promotes prostate cancer cell proliferation, invasion and migration and inhibit apoptosis. In addition, the androgen receptor directly regulated the transcription of miR-182-5p, which could target to the 3'UTR of ARRDC3 mRNA and affect the expression of ARRDC3 and its downstream gene ITGB4. For the in vivo experiment, miR-182-5p overexpression also promoted the growth and progression of prostate cancer tumors. In this regard, we suggest that miR-182-5p may be a key androgen receptor-regulated factor that contributes to the development and metastasis of Chinese prostate cancers and may be a potential target for the early diagnosis and therapeutic studies of prostate cancer.
Department of Urology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, 200433, PR China.
Jingjing
J
Yao
Research Center of Developmental Biology, Second Military Medical University, 800th Xiangyin Road, Shanghai, 200433, PR China; Department of Orthopedics, Changzheng Hospital Affiliated to Second Military Medical University, 415th Feng Yang Road, Shanghai, 200003, PR China.
Chen
C
Xu
Department of Urology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, 200433, PR China.
Ziyu
Z
Fang
Department of Urology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, 200433, PR China.
Yaoming
Y
Li
Research Center of Developmental Biology, Second Military Medical University, 800th Xiangyin Road, Shanghai, 200433, PR China; Translational Medicine Center, Second Military Medical University, 800th Xiangyin Road, Shanghai, 200433, PR China.
Houqi
H
Liu
Research Center of Developmental Biology, Second Military Medical University, 800th Xiangyin Road, Shanghai, 200433, PR China; Translational Medicine Center, Second Military Medical University, 800th Xiangyin Road, Shanghai, 200433, PR China.
Yue
Y
Wang
Department of Urology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, 200433, PR China.
Chuanliang
C
Xu
Department of Urology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, 200433, PR China. Electronic address: sunyh@medmail.com.cn.
Yinghao
Y
Sun
D019390:Arrestins / Q000235:genetics*; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D039663:Integrin beta4 / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D011944:Receptors, Androgen / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
C508469:ARRDC3 protein, human; D019390:Arrestins; C059834:ITGB4 protein, human; D039663:Integrin beta4; D035683:MicroRNAs; C538984:Mirn182 microRNA, human; D011944:Receptors, Androgen
ARRDC3; Androgen receptor; ITGB4; miR-182-5p; microRNA