Phenylobacterium panacis sp. nov., isolated from the rhizosphere of rusty mountain ginseng.
A novel, Gram-stain-negative, rod-shaped bacterial strain, designated as DCY109T, was isolated from the rhizosphere of rusty mountain ginseng root located on Hwacheon mountain of Gangwon province, South Korea. 16S rRNA gene sequence analysis revealed that strain DCY109Tbelonged to the genus Phenylobacterium and was related closely to Phenylobacterium muchangponense KACC 15042T(98.2 % similarity), Phenylobacterium immobile DSM 1986T(96.9 %) and Phenylobacterium koreense KCTC 12206T(96.7 %). The predominant isoprenoid quinone was ubiquinone (Q-10) and the DNA G+C content was 66.9 mol%. The major polar lipids were phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The major fatty acids (>10 %) were C16 : 0, summed feature 3 (which comprised C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (which comprised C18 : 1ω7c and/or C18 : 1ω6c). Mean DNA-DNA relatedness between strain DCY109Tand its closest relative, P. muchangponense KACC 15042T, was 15.1±3.9 %. Based on the physiological, biochemical, chemotaxonomic and genetic analyses, strain DCY109Tis considered to represent a novel species of the genus Phenylobacterium, for which the name Phenylobacterium panacis sp. nov. is proposed. The type strain is DCY109T(=KCTC 42749T=JCM 31045T).
GraduateSchool of Biotechnology and Ginseng Bank, College of Life Science, KyungHee University, Yongin, 446-701, Republic of Korea.
Mohamed El-Agamy
ME
Farh
GraduateSchool of Biotechnology and Ginseng Bank, College of Life Science, KyungHee University, Yongin, 446-701, Republic of Korea.
Yeon-Ju
YJ
Kim
Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea.
Priyanka
P
Singh
Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea.
Van-An
VA
Hoang
GraduateSchool of Biotechnology and Ginseng Bank, College of Life Science, KyungHee University, Yongin, 446-701, Republic of Korea.|Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea.
Deok-Chun
DC
Yang
D015373:Bacterial Typing Techniques; D042063:Caulobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D005894:Panax / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2
Phenylobacterium panacissp. nov; Proteobacteria; Taxonomy; rusty ginseng roots