Seinpin knockout exacerbates cerebral ischemia/reperfusion damage in mice.
Seipin, which regulates adipocyte differentiation and lipolysis, inducing severe lipodystrophy and metabolic syndromes, is also highly expressed in the nervous system and affects some neurological diseases. However, the impacts of seipin in stroke remain unclear.



In this study, we subjected seipin knockout mice to cerebral ischemia/reperfusion injury and found that seipin knockout mice exhibited exacerbated neurological disorder and enlarged infarct size, companied by blood-brain barrier (BBB) damages. Furthermore, we showed that seipin knockout aggravated endoplasmic reticulum (ER) stress and significantly increased glucose levels, decreased leptin and adiponectin levels in mouse plasma.



Our findings reveal that seipin knockout exacerbates cerebral I/R-induced damages by increasing BBB permeability, amplifying ER stress and increasing glucose levels, as well as decreasing leptin and adiponectin levels, indicating that seipin may be a potential therapeutic target for stroke.
Department of Neurology, People's Hospital of Deyang City, Taishian North Road 173, Deyang City, 618000, China.
Yong
Y
Chen
Institute of Cardiovascular Sciences, Peking University Health Science Center, 38 Xueyuan Rd, Hai Dian District, 100083, Beijing, China.
Lili
L
Wei
Department of Neurology, People's Hospital of Deyang City, Taishian North Road 173, Deyang City, 618000, China.
Jing
J
Tian
Institute of Cardiovascular Sciences, Peking University Health Science Center, 38 Xueyuan Rd, Hai Dian District, 100083, Beijing, China.
Yu-Hui
YH
Wang
Institute of Cardiovascular Sciences, Peking University Health Science Center, 38 Xueyuan Rd, Hai Dian District, 100083, Beijing, China. Electronic address: vangeorgeliu@gmail.com.
George
G
Liu
Department of Neurology, People's Hospital of Deyang City, Taishian North Road 173, Deyang City, 618000, China. Electronic address: ronaldnju@gmail.com.
Chun
C
Wang
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism*; D044388:GTP-Binding Protein gamma Subunits; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008060:Lipodystrophy / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology
C115783:Bscl2 protein, mouse; D044388:GTP-Binding Protein gamma Subunits; D020962:Heterotrimeric GTP-Binding Proteins
Ischemic stroke; Knockout mice; Lipodystrophy; Seipin; Transient middle cerebral artery occlusion