Fabrication and characterization of Mg-doped chitosan-gelatin nanocompound coatings for titanium surface functionalization.
Titanium and its alloys have been widely used in clinic and achieved great success. Due to the bio-inertness of titanium surface, challenges still exit in some compromised conditions. The present study aimed to functionalize titanium surface with magnesium (Mg)-doped chitosan/gelatin (CS/G) nanocompound coatings via electrophoretic deposition (EPD). CS/G coatings loaded with different amount of magnesium were successfully prepared on titanium substrate via EPD. Physicochemical characterization of the coatings confirmed that magnesium ions were loaded into the coatings in a dose-dependent manner. XRD results demonstrated that co-deposition of magnesium influenced the crystallinity of the coatings, and a new crystalline substance presented, namely hydrated basic magnesium carbonate. Mechanical tests showed improved tensile and shear bond strength of the magnesium-doped coatings, while the excessively high magnesium concentration could eventually decrease the bonding strength. Sustained release of magnesium ion was detected by ICP-OES within 28 days. TEM images also displayed that nanoparticles could be released from the coatings. In vitro cellular response assays demonstrated that the Mg-doped nanocompound coatings could enhance the proliferation and osteogenic differentiation of MC3T3-E1 cells compared to CS/G coatings. Therefore, it could be concluded that Mg-doped CS/G nanocompound coatings were successfully fabricated on titanium substrates via EPD. It would be a promising candidate to functionalize titanium surface with such organic-inorganic nanocompound coatings.
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.
Xinjie
X
Cai
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.
Jing
J
Cai
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.|b Department of Prosthodontics, Hospital of Stomatology , Wuhan University , Wuhan , China.
Kena
K
Ma
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.
Pin
P
Huang
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.
Lingling
L
Gong
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.
Dan
D
Huang
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.|b Department of Prosthodontics, Hospital of Stomatology , Wuhan University , Wuhan , China.
Tao
T
Jiang
a The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University , Wuhan , China.|b Department of Prosthodontics, Hospital of Stomatology , Wuhan University , Wuhan , China.
Yining
Y
Wang
D016475:3T3 Cells; D000818:Animals; D002118:Calcium / Q000737:chemistry; D002454:Cell Differentiation; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D019736:Prostheses and Implants; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; C005479:magnesium carbonate; D005780:Gelatin; D048271:Chitosan; D014025:Titanium; D008274:Magnesium; D002118:Calcium
Magnesium; chitosan; coating; electrophoretic deposition; functionalization; gelatin