Endothelialization of polyurethanes: Surface silanization and immobilization of REDV peptide.
The paper presents method for chemical immobilization of arginine-glutamic acid-aspartic acid-valine (REDV) peptide on polyurethane surface. The peptide has been covalently bonded using silanes as a spacer molecules. The aim of this work was to investigate the proposed modification process and assess its biological effectiveness, especially in contact with blood and endothelial cells. Physicochemical properties were examined in terms of wettability, atomic composition and density of introduced functional groups and peptide molecules. Experiments with blood showed that material coating reduced number of surface-adhered platelets and fibrinogen molecules. In contrast to polyurethane (PU), there were no blood components deposited on REDV-modified materials (PU-REDV); fibrinogen adsorption on PU-REDV surface has been strongly reduced compared to PU. Analysis of cell adhesion after 1, 2, 3, 4, and 5 days of culture showed a significant increase of the cell-coated area on PU-REDV compared to PU. However, an intense cell growth appeared also on the control surface modified without the addition of REDV. Thus, the positive effect of REDV peptide on the adhesion of HUVEC could not be unequivocally confirmed.
Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland. Electronic address: b.butruk@ichip.pw.edu.pl.
Beata A
BA
Butruk-Raszeja
Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland.
Magdalena S
MS
Dresler
Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland.
Aleksandra
A
Kuźmińska
Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland; CEZAMAT PW, Polna 50, 00-644 Warsaw, Poland.
Tomasz
T
Ciach
D000327:Adsorption; D000328:Adult; D000838:Anions; D002412:Cations; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D055767:Immobilized Proteins / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry / Q000494:pharmacology*; D012821:Silanes / Q000737:chemistry*; D013499:Surface Properties; D017155:Wettability
D000838:Anions; D002412:Cations; D055767:Immobilized Proteins; D009842:Oligopeptides; D011140:Polyurethanes; D012821:Silanes; C000610191:arginyl-glutamyl-aspartyl-valine; D005340:Fibrinogen
Blood compatibility; Endothelial cells; Platelet adhesion; Polyurethane; REDV; Surface endothelialization