Prevalence of Dientamoeba fragilis, Giardia duodenalis, Entamoeba histolytica/dispar, and Cryptosporidium spp in Da Nang, Vietnam, detected by a multiplex real-time PCR.
We surveyed the prevalence of Dientamoeba fragilis, Giardia duodenalis, Entamoeba histolytica, Entamoeba dispar, and Cryptosporidium spp in individuals with and without gastrointestinal (GI) symptoms residing in and around Da Nang city, Vietnam. Fecal samples were collected from children (n = 100) and adults (n = 80) with GI symptoms and from healthy individuals (n = 88) reporting no GI symptoms. Parasite detection was performed by multiplex real-time PCR. Overall, except for G. duodenalis, we found a low prevalence (<5%) of D. fragilis and E. dispar and no detection of E. histolytica and C. spp in all participants with GI symptoms. Specifically for D. fragilis this contrasts with findings in European populations of children with GI symptoms showing prevalence up to 73%. Moreover, our results indicate that the prevalence of G. duodenalis is higher in patients with GI symptoms compared to asymptomatic individuals and this difference is most obvious in young patients.
Division of Medical Diagnostics, Clinical Microbiology Laboratory, County Hospital Ryhov, Region Jönköping County, Sweden.
Jessica
J
Ögren
Department of Medical Laboratory, Da Nang University of Medical Technology and Pharmacy, Da Nang, Vietnam.
Song
S
Van Nguyen
Department of Medical Laboratory, Da Nang University of Medical Technology and Pharmacy, Da Nang, Vietnam.
Minh Khac
MK
Nguyen
Department of Natural Science and Biomedicine, School of Health and Welfare, Jönköping University, Jönköping, Sweden.
Jan
J
Dimberg
Division of Medical Diagnostics, Clinical Microbiology Laboratory, County Hospital Ryhov, Region Jönköping County, Sweden.
Andreas
A
Matussek
D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification*; D004029:Dientamoeba / Q000235:genetics / Q000302:isolation & purification*; D004747:Entamoeba / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D005260:Female; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007411:Intestinal Diseases, Parasitic / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D014744:Vietnam / Q000453:epidemiology; D055815:Young Adult
Dientamoeba fragilis; Entamoeba histolytica/dispar and Cryptosporidium spp; Giardia duodenalis; PCR; prevalence