Expression and regulatory effects of microRNA-182 in osteosarcoma cells: A pilot study.
The aim of the present study was to evaluate the expression level of microRNA-182 (miRNA-182) in human osteosarcoma (OS) MG-63 cells and OS tissues, and to elucidate the effect of miRNA-182 on the biological activity of tumors. In the present study, the expression of miRNA-182 in human OS MG-63 cells, OS tissues and normal osteoblast hFOB1.19 cells was determined using quantitative polymerase chain reaction. Subsequently, a miRNA-182 mimic and inhibitor were utilized to regulate the expression level of this miRNA in MG-63 cells. Cell viability and proliferation were examined using cell counting kit-8 assays, and cell apoptosis was detected by flow cytometry. Cell invasion and migration assays were performed using Transwell chambers to analyze the biological functions of miRNA-182in vitro. The present study demonstrated that the expression level of miRNA-182 in MG-63 cells and OS tissues was significantly increased compared with the hFOB1.19 cell line (P<0.05). The present study successfully performed cell transfections of miRNA-182 inhibitor and miRNA-182 mimic into MG-63 cells and achieved the desired transfection efficiency. The present study confirmed that upregulation of miRNA-182 promotes cell apoptosis and inhibits cell viability, proliferation, invasion and migration. The present findings additionally demonstrated that miRNA-182 is a tumor suppressor gene in OS. Therefore, regulating the expression of miRNA-182 may affect the biological behavior of OS cells, which suggests a potential role for miRNA-182 in molecular therapy for malignant tumors.
Department of Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Dong-Lin
DL
Bian
Department of Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Xue-Mei
XM
Wang
Department of Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Kun
K
Huang
Department of Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Qi-Xi
QX
Zhai
Key Laboratory of Diagnosis and Interventional Therapy of Liaoning Province, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Gui-Bo
GB
Yu
Department of Radiology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Cheng-Hua
CH
Wu
biological behaviors of neoplasms; gene expression; microRNA-182; osteosarcoma