Leojaponic acids A and B, two new homologous terpenoids, isolated from Leonurus japonicus.
The present study aimed at isolation and purification of the bioactive terpenoids from the herb of Leonurus japonicus by chromatographic separations such as silica gel, sephadex LH-20 and C18 reversed phase silica gel, as well as preparative HPLC. As a result, leojaponic acids A (1, C17H24O4) and B (2, C18H26O4), two homologous terpenoids, together with (-)-loliolide (3), 1-(3-ethylphenyl) ethane-1, 2-diol (4) and dibutyl phthalate (5), were isolated from the EtOH extract of L. japonicus. All the chemical structures of the isolates were elucidated on the basis of 1D and 2D NMR analyses. Compounds 1 and 2 were new terpenoids, and Compounds 3 and 4 were isolated and identified for the first time from this plant. In addition, the α-glucosidase and tyrosinase inhibitory activity of the new compounds were evaluated.
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China. Electronic address: hkwu@aynu.edu.cn.
Han-Kui
HK
Wu
Institute of Analytical Science, Northwest University, Xi'an 710069, China.
Yan-Jun
YJ
Mao
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Shan-Shan
SS
Sun
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Zhi-Yong
ZY
Xu
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Ya
Y
Ma
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Jin-Xia
JX
Cao
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
He
H
Qi
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Zhi-Fu
ZF
Wu
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Gang
G
Li
Institute of Cotton Research, Chinese Academy of Agriculture Sciences, Anyang 455000, China. Electronic address: cottontest@126.com.
Wei-Hua
WH
Yang
D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D005959:Glucosidases / Q000032:analysis / Q000037:antagonists & inhibitors; D031332:Leonurus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013729:Terpenes; D005959:Glucosidases
Leojaponic acid; Leonurus japonicus; Terpenoid