Brucella abortus-infected B cells induce osteoclastogenesis.
Brucella abortus is an intracellular bacterium that establishes lifelong infections in livestock and humans although the mechanisms of its chronicity are poorly understood. Activated B cells have long lifespan and B. abortus infection activates B cells. Our results indicate that the direct infection of B cells with B. abortus induced matrix metalloproteinase-9 (MMP-9), receptor activator for NF κB ligand (RANKL), tumor necrosis factor (TNF)-α and interleukin (IL)-6 secretion. In addition, supernatants from B. abortus-infected B cells induced bone marrow-derived monocytes to undergo osteoclastogenesis. Using osteoprotegerin, RANKL's decoy receptor, we determined that RANKL is involved in osteoclastogenesis induced by supernatants from B. abortus-infected B cells. The results presented here shed light on how the interactions of B. abortus with B cells may have a role in the pathogenesis of brucellar osteoarticular disease.
Instituto de Inmunología, Genética y Metabolismo (INIGEM), Hospital de Clínicas "José de San Martín", Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
Ayelén Ivana
AI
Pesce Viglietti
Instituto de Inmunología, Genética y Metabolismo (INIGEM), Hospital de Clínicas "José de San Martín", Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
Paula Constanza
PC
Arriola Benitez
Instituto de Inmunología, Genética y Metabolismo (INIGEM), Hospital de Clínicas "José de San Martín", Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
Guillermo Hernán
GH
Giambartolomei
Instituto de Inmunología, Genética y Metabolismo (INIGEM), Hospital de Clínicas "José de San Martín", Facultad de Medicina, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina. Electronic address: mdelpino@ffyb.uba.ar.
María Victoria
MV
Delpino
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology*; D002003:Brucella abortus / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D008213:Lymphocyte Activation; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010012:Osteogenesis; D053245:RANK Ligand / Q000378:metabolism
D016207:Cytokines; D053245:RANK Ligand; C505688:TNFSF11 protein, human
B cells; Brucella abortus; Osteoclastogenesis