Comparison of two silica based nonviral gene therapy vectors for breast carcinoma: evaluation of the p53 delivery system in Balb/c mice.
Silica nanoparticles as a nonviral vector for in vivo gene therapy neither surface functionalized SiNp1 is neither "a cationic ion" nor a surface (encapsulation) nor SiNp2 (adsorption). p53 gene expression in the breast upon (i.v) administration. SiNp1 showed a 50- and 100-fold transfection activity, tumor growth inhibition, animal survival (80%), and high levels of p53 and Bax were detected in the sera of treated animals compared to SiNp2 or naked pCMV/p53, respectively. These results demonstrate for improvements in the both systems. This study suggests that nonviral vector systems will have important roles in achieving the impermanent gene transfer in vivo.
a Department of Zoology, Proteomics and Molecular Cell Physiology Lab , School of Life Sciences, Bharathiar University , Coimbatore , Tamil Nadu , India.|b School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai , China.
Chandrababu
C
Rejeeth
a Department of Zoology, Proteomics and Molecular Cell Physiology Lab , School of Life Sciences, Bharathiar University , Coimbatore , Tamil Nadu , India.|b School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai , China.
Raju
R
Vivek
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015552:Injections, Intralesional; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D009368:Neoplasm Transplantation; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011437:Propylamines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism; D016019:Survival Analysis; D047368:Tumor Burden; D016159:Tumor Suppressor Protein p53 / Q000819:agonists / Q000097:blood / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000819:agonists / Q000097:blood / Q000235:genetics
C494826:BAX protein, human; D008823:Micelles; D011437:Propylamines; D012821:Silanes; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D012822:Silicon Dioxide; C052671:vinyltriethoxysilane; C477625:amino-propyl-triethoxysilane
Breast cancer; gene delivery; in vivo transfection; pCMV/p53; silica nanoparticles