The chemopreventive potential of lycopene against atrazine-induced cardiotoxicity: modulation of ionic homeostasis.
People who drink water contaminated with atrazine (ATR) over many years can experience problems with their cardiovascular system. Lycopene (LYC) has been shown to exhibit cardiovascular disease preventive effects. However, chemopreventive potential of LYC against ATR-induced cardiotoxicity remains unclear. To determine the effects of ATR and/or LYC on heart, mice were treated with ATR (50 mg/kg or 200 mg/kg) and/or LYC (5 mg/kg) by intragastric administration for 21 days. Histopathological and biochemical analyses, including analysis of ion concentrations (Na(+), K(+), Ca(2+) and Mg(2+)), ATPases (Na(+)-K(+)-ATPase, Ca(2+)-ATPase, Mg(2+)-ATPase and Ca(2+)-Mg(2+)-ATPase) activities and the transcription of their subunits, were performed on heart. The results revealed that ATR led to decreased Creative Kinase (CK) activity and increased histological alterations. Furthermore, a significant change in Na(+), K(+) and Ca(2+) content and the down-regulation of Na(+)-K(+)-ATPase and Ca(2+)-ATPase activities and the mRNA expression of their subunits were observed in ATR-exposed mice. Notably, supplementary LYC significantly protected the heart against ATR-induced damage. In conclusion, ATR induced cardiotoxicity by modulating cardiac ATPase activity and the transcription of its subunits, thereby triggering ionic disturbances. However, supplementary LYC significantly combated ATR-induced cardiotoxicity via the regulation of ATPase activity and subunit transcription. Thus, LYC exhibited a significant chemopreventive potential against ATR-induced cardiotoxicity.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Jia
J
Lin
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Hui-Xin
HX
Li
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Jun
J
Xia
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Xue-Nan
XN
Li
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Xiu-Qing
XQ
Jiang
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Shi-Yong
SY
Zhu
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Jing
J
Ge
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Jin-Long
JL
Li
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001280:Atrazine / Q000633:toxicity*; D001671:Biochemistry; D066126:Cardiotoxicity / Q000517:prevention & control*; D002338:Carotenoids / Q000008:administration & dosage*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006540:Herbicides / Q000633:toxicity*; D006651:Histocytochemistry; D006706:Homeostasis; D007477:Ions / Q000378:metabolism*; D000077276:Lycopene; D051379:Mice; D016896:Treatment Outcome
D000975:Antioxidants; D006540:Herbicides; D007477:Ions; D002338:Carotenoids; D001280:Atrazine; D000077276:Lycopene