Imbalanced Macrophage and Dendritic Cell Activations in Response to Candida albicans in a Murine Model of Diabetes Mellitus.
Bloodstream infections caused by Candida species are responsible for high morbidity and mortality, and diabetes mellitus (DM) is an important underlying disease in candidemia episodes. Although DM patients show an enhanced proinflammatory profile, they are highly susceptible to mycobacterial and mycotic infections. Attempting to understand this paradox, we investigated if imbalanced macrophage and dendritic cell (DC) activations could be associated to high incidence and/or severity of Candida albicans infection in the hypoinsulinemia-hyperglycemia (HH) milieu. HH alloxan-induced mice were infected with C. albicans and peritoneal aderent phagocytes were co-cultured with or without lipopolyssaccharide or heat-killed C. albicans, and the production of cytotoxic metabolites, cytokines, and chemokines was evaluated. We also evaluated the surface expression of MHC-II and CD86 in splenic DCs. Our findings showed that both uninfected and C. albicans-infected HH mice showed less production of CCL2 and reduced expression of CD86 by peritoneal phagocytes and splenic DCs, respectively.
a Faculdade de Ciências , UNESP - Universidade Estadual Paulista , Bauru , SP , Brazil.
James
J
Venturini
a Faculdade de Ciências , UNESP - Universidade Estadual Paulista , Bauru , SP , Brazil.|b Instituto de Biocências de Botucatu , Universidade Estadual Paulista , Botucatu , SP , Brazil.
Thais Fernanda Campos
TF
Fraga-Silva
a Faculdade de Ciências , UNESP - Universidade Estadual Paulista , Bauru , SP , Brazil.
Camila Martins
CM
Marchetti
a Faculdade de Ciências , UNESP - Universidade Estadual Paulista , Bauru , SP , Brazil.|b Instituto de Biocências de Botucatu , Universidade Estadual Paulista , Botucatu , SP , Brazil.
Luiza Ayumi Nishiyama
LA
Mimura
b Instituto de Biocências de Botucatu , Universidade Estadual Paulista , Botucatu , SP , Brazil.
Bruno José
BJ
Conti
c Faculdade de Medicina de Botucatu , UNESP - Universidade Estadual Paulista , Botucatu , SP , Brazil.
Márjorie de Assis
Mde A
Golim
c Faculdade de Medicina de Botucatu , UNESP - Universidade Estadual Paulista , Botucatu , SP , Brazil.
Rinaldo Poncio
RP
Mendes
a Faculdade de Ciências , UNESP - Universidade Estadual Paulista , Bauru , SP , Brazil.
Maria Sueli Parreira
MS
de Arruda
D000496:Alloxan / Q000633:toxicity; D000818:Animals; D051940:B7-2 Antigen / Q000378:metabolism; D001938:Brazil; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000382:microbiology*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000382:microbiology; D005802:Genes, MHC Class II / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice
D051940:B7-2 Antigen; C491768:Ccl2 protein, mouse; C497965:Cd86 protein, mouse; D018932:Chemokine CCL2; D000496:Alloxan
Alloxan; candidiasis; dendritic cells; experimental diabetes mellitus; macrophage activity