Morphological and molecular description of immature stages of Ornithodoros savignyi (Acari: Argasidae).
This study was designed to provide more details about larva, first nymph, and second nymph of Ornithodoros savignyi using a combination of light microscopy (LM), scanning electron microscopy (SEM), and partial sequence of mitochondrial 16S ribosomal ribonucleic acid (rRNA). The main characteristics of larva are wrinkled integument with many grooves, gnathosoma without camerostome cheeks, hypostome with a pair of large teeth apically, and tarsus without humps. The comparisons between the first and the second nymphs are different shape and distribution of dorsal grooves; a few spots without mammilla on the dorsal surface of the second nymph; 27 and 63-65 pairs of setae on the dorsal surface of the first and second nymphs, respectively; small holes on mammillae that are more dense in the second nymph; basis capitulum with two pairs of small setae in the second nymph; and one pair of sate in the first nymph, hypostome with dental formula 2/2 in the first nymph, and 3/3 in the second nymph. The partial 16S rRNA sequence of the second nymph that was determined as O. savignyi (450 bp) was deposited in GenBank under the accession number KU163242.
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt. aasobhy@yahoo.com.
Sobhy
S
Abdel-Shafy
Department of Agricultural Zoology, Faculty of Agriculture, Cairo University, Giza, Egypt.
Hanan S M
HS
Gabr
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt.
Hend H A M
HH
Abdullah
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt.
Mona S
MS
Mahmoud
D000818:Animals; D005260:Female; D007814:Larva / Q000033:anatomy & histology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000235:genetics; D009758:Nymph / Q000033:anatomy & histology*; D026861:Ornithodoros / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics; D012313:RNA / Q000235:genetics; D000077278:RNA, Mitochondrial; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D000077278:RNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D012313:RNA
Description; Larva; Molecular analysis; Morphology; Nymph; Taxonomy