Dodecylresorufin (C12R) Outperforms Resorufin in Microdroplet Bacterial Assays.
This paper proves that dodecylresorufin (C12R) outperforms resorufin (the conventional form of this dye) in droplet microfluidic bacterial assays. Resorufin is a marker dye that is widely used in different fields of microbiology and has increasingly been applied in droplet microfluidic assays and experiments. The main concern associated with resorufin in droplet-based systems is dye leakage into the oil phase and neighboring droplets. The leakage decreases the performance of assays because it causes averaging of the signal between the positive (bacteria-containing) and negative (empty) droplets. Here we show that C12R is a promising alternative to conventional resorufin because it maintains higher sensitivity, specificity, and signal-to-noise ratio over time. These characteristics make C12R a suitable reagent for droplet digital assays and for monitoring of microbial growth in droplets.
Institute of Physical Chemistry, Polish Academy of Sciences , Kasprzaka 44/52, 01-224 Warsaw, Poland.|Institute of Molecular and Cell Biology, University of Tartu , Riia 23, 51010 Tartu, Estonia.
Ott
O
Scheler
Institute of Physical Chemistry, Polish Academy of Sciences , Kasprzaka 44/52, 01-224 Warsaw, Poland.
Tomasz S
TS
Kaminski
Institute of Physical Chemistry, Polish Academy of Sciences , Kasprzaka 44/52, 01-224 Warsaw, Poland.
Artur
A
Ruszczak
Institute of Physical Chemistry, Polish Academy of Sciences , Kasprzaka 44/52, 01-224 Warsaw, Poland.
Piotr
P
Garstecki
D001681:Biological Assay; D044085:Microfluidics; D010078:Oxazines; D059629:Signal-To-Noise Ratio
D010078:Oxazines; C014180:resorufin
bacteria viability assay; dodecylresorufin; droplet emulsion; droplet microfluidics; resorufin