Blue light reduces organ injury from ischemia and reperfusion.
Evidence suggests that light and circadian rhythms profoundly influence the physiologic capacity with which an organism responds to stress. However, the ramifications of light spectrum on the course of critical illness remain to be determined. Here, we show that acute exposure to bright blue spectrum light reduces organ injury by comparison with bright red spectrum or ambient white fluorescent light in two murine models of sterile insult: warm liver ischemia/reperfusion (I/R) and unilateral renal I/R. Exposure to bright blue light before I/R reduced hepatocellular injury and necrosis and reduced acute kidney injury and necrosis. In both models, blue light reduced neutrophil influx, as evidenced by reduced myeloperoxidase (MPO) within each organ, and reduced the release of high-mobility group box 1 (HMGB1), a neutrophil chemotactant and key mediator in the pathogenesis of I/R injury. The protective mechanism appeared to involve an optic pathway and was mediated, in part, by a sympathetic (β3 adrenergic) pathway that functioned independent of significant alterations in melatonin or corticosterone concentrations to regulate neutrophil recruitment. These data suggest that modifying the spectrum of light may offer therapeutic utility in sterile forms of cellular injury.
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213; The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China;
Du
D
Yuan
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213;
Richard D
RD
Collage
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213;
Hai
H
Huang
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213;
Xianghong
X
Zhang
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213;
Benjamin C
BC
Kautza
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213;
Anthony J
AJ
Lewis
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213;
Brian S
BS
Zuckerbraun
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213;
Allan
A
Tsung
Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 15261.
Derek C
DC
Angus
Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213; Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 15261 rosengartmr@upmc.edu.
Matthew R
MR
Rosengart
D000818:Animals; D003116:Color; D016500:Color Therapy / Q000379:methods*; D003345:Corticosterone / Q000097:blood*; D004307:Dose-Response Relationship, Radiation; D024243:HMGB1 Protein / Q000097:blood; D007677:Kidney Function Tests; D008111:Liver Function Tests; D008297:Male; D008550:Melatonin / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D009195:Peroxidase / Q000097:blood; D011829:Radiation Dosage; D015427:Reperfusion Injury / Q000175:diagnosis / Q000503:physiopathology* / Q000517:prevention & control*; D012720:Severity of Illness Index; D016896:Treatment Outcome
D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D009195:Peroxidase; D008550:Melatonin; D003345:Corticosterone
blue light; circadian rhythms; ischemia; organ injury; reperfusion