Polyphyllin D induces apoptosis and differentiation in K562 human leukemia cells.
Polyphyllin D, a compound derived from Paris polyphylla rhizoma, demonstrated strong anticancer activities in a previous study. Our results demonstrated that polyphyllin D exerts a growth inhibitory effect by inducing apoptosis and differentiation in the human erythroleukemia cell line K562. Polyphyllin D induced apoptosis via the mitochondrial apoptotic pathway, as evidenced by the decreased Bcl-2 and Bcr/Abl expression levels, the disruption of MMP and increased Bax, cytochrome c and cleaved-caspase-3 levels. At a low dose, polyphyllin D increased CD14 expression on the surface of K562 cells and induced cells to differentiate into monocytes or mature macrophages. These data suggest that polyphyllin D has the potential to be a potent therapeutic agent for treating human chronic myelogenous leukemia.
Clinical Laboratory, The Second Hospital of Dalian Medical University, Dalian 116023, China.
Chunhui
C
Yang
Clinical Laboratory, The Second Hospital of Dalian Medical University, Dalian 116023, China.
Hong
H
Cai
Department of Laboratory Hematology, College of Laboratory Medicine, Dalian Medical University, Dalian 116023, China. Electronic address: xiuxiang_meng@sina.com.
Xiuxiang
X
Meng
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000494:pharmacology; D016313:Genes, abl / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy*; D019668:Liliaceae / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D008516:Medicine, Chinese Traditional; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D051562:Proto-Oncogene Proteins c-bcr / Q000235:genetics; D012503:Saponins / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D019253:Proto-Oncogene Proteins c-bcl-2; D012503:Saponins; C121203:polyphyllin D; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr; D053148:Caspase 3; D004144:Diosgenin
Apoptosis; Differentiation; K562; Polyphyllin D