Electrical detection of dengue virus (DENV) DNA oligomer using silicon nanowire biosensor with novel molecular gate control.
In this paper, a silicon nanowire biosensor with novel molecular gate control has been demonstrated for Deoxyribonucleic acid (DNA) detection related to dengue virus (DENV). The silicon nanowire was fabricated using the top-down nanolithography approach, through nanostructuring of silicon-on-insulator (SOI) layers achieved by combination of the electron-beam lithography (EBL), plasma dry etching and size reduction processes. The surface of the fabricated silicon nanowire was functionalized by means of a three-step procedure involving surface modification, DNA immobilization and hybridization. This procedure acts as a molecular gate control to establish the electrical detection for 27-mers base targets DENV DNA oligomer. The electrical detection is based on the changes in current, resistance and conductance of the sensor due to accumulation of negative charges added by the immobilized probe DNA and hybridized target DNA. The sensitivity of the silicon nanowire biosensors attained was 45.0µAM(-1), which shows a wide-range detection capability of the sensor with respect to DNA. The limit of detection (LOD) achieved was approximately 2.0fM. The demonstrated results show that the silicon nanowire has excellent properties for detection of DENV with outstanding repeatability and reproducibility performances.
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia. Electronic address: m.nuzaihan@unimap.edu.my.
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Nuzaihan M N
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis (UniMAP), 02600 Pauh, Perlis, Malaysia.
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Hashim
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis (UniMAP), 02600 Pauh, Perlis, Malaysia.
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Md Arshad
School of Microelectronic Engineering, Universiti Malaysia Perlis (UniMAP), 02600 Pauh, Perlis, Malaysia.
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Kasjoo
Chemistry Department, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
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Rahman
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia.
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Ruslinda
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia.
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Fathil
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia.
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Adzhri
School of Microelectronic Engineering, Universiti Malaysia Perlis (UniMAP), 02600 Pauh, Perlis, Malaysia.
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Shahimin
D015374:Biosensing Techniques / Q000295:instrumentation*; D004279:DNA, Viral / Q000032:analysis*; D003715:Dengue / Q000175:diagnosis / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification*; D004867:Equipment Design; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012825:Silicon / Q000737:chemistry*; D014159:Transducers
D004279:DNA, Viral; D061925:Immobilized Nucleic Acids; D012825:Silicon
Dengue fever (DF); Deoxyribonucleic acid (DNA); Electrical detection; Molecular gate control; Nanolithography; Silicon nanowire biosensor