Angiomodulatory properties of some antibiotics and Tołpa Peat Preparation.
Deterioration of the immune system due to antibiotic therapy can be restored by immunomodulator application. In this paper we estimate the effect of ampicillin, amikacin, doxycycline, rifampicin, rifamycine and immunomodulator Tołpa Peat Preparation (TPP) on neovascular reaction induced in murine skin by human mononuclear cells (MNC) injection. MNC originating from 15 healthy volunteers were injected intradermally to Balb/c mice. Antibiotics (3, 15, or 75 mg/kg of body weight) alone or with TPP (10 mg/kg of body weight) were administrated subcutaneously to mice on three consecutive days. The number of newly formed blood vessels was measured in dissection microscope 72 hours after cell injection.


RESULTS
TPP stimulated angiogenic activity of MNC at the dose 5 and 10 mg/kg. Rifamycine exerted strong stimulatory action, ampicillin slightly stimulated immune response, while doxycycline and rifampicin downregulated it. Amikacin did not influence the results of angiogenesis tests. Studied antibiotics (15 mg/kg), except rifamycine, inhibit the angiostimulatory effect of the tested immunomodulator. TPP should be applied after antibiotic therapy to maintain its stimulatory effect and restore proper host immune function.
Department of Histology and Embryology, Biostructure Centre, Medical University of Warsaw, Warsaw, Poland.
Dorota M
DM
Radomska-Leśniewska
Department of Pathomorphology, Biostructure Centre, Medical University of Warsaw, Warsaw, Poland; Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Ewa
E
Skopińska-Różewska
Department of Histology and Embryology, Biostructure Centre, Medical University of Warsaw, Warsaw, Poland.
Jarosław
J
Jóźwiak
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland.
Urszula
U
Demkow
Department of Immunology, Biochemistry, and Nutrition, Medical University of Warsaw, Warsaw, Poland.
Barbara
B
Joanna Bałan
Tołpa Peat Preparation (TPP); amikacin; ampicillin; angiogenesis; antibiotics; doxycycline; immune system; rifampicin; rifamycine