β-Asarone Inhibits IRE1/XBP1 Endoplasmic Reticulum Stress Pathway in 6-OHDA-Induced Parkinsonian Rats.
Parkinson's disease (PD) is a neurodegenerative disease, with genetics and environment contributing to the disease onset. The limited pathological cognize of the disease restrained the approaches to improve the clinical treatment. Recently, studies showed that endoplasmic reticulum (ER) stress played an important role in the pathogenesis of PD. There was a neuroprotective effect partly mediated by modulating ER stress. β-Asarone is the essential constituent of Acorus tatarinowii Schott volatile oil. Our team observed that β-asarone could improve the behavior of parkinsonian rats; increase the HVA, Dopacl, and 5-HIAA levels; and reduce α-synuclein levels. Here we assumed that the protective role of β-asarone on parkinsonian rats was mediated via ER stress pathway. To prove the hypothesis we investigated the mRNA levels of glucose regulated protein 78 (GRP78) and C/EBP homologous binding protein (CHOP) in 6-hydroxy dopamine (6-OHDA) induced parkinsonian rats after β-asarone treatment. Furthermore, the inositol-requiring enzyme 1/X-Box Binding Protein 1 (IRE1/XBP1) ER stress pathway was also studied. The results showed that β-asarone inhibited the mRNA levels of GRP78 and CHOP, accompanied with the delined expressions of phosphorylated IER1 (p-IRE1) and XBP1. We deduced that β-asarone might have a protective effect on the 6-OHDA induced parkinsonian rats via IRE1/XBP1 Pathway. Collectively, all data indicated that β-asarone might be a potential candidate of medicine for clinical therapy of PD.
The Guangzhou University of Chinese Medicine, Guangzhou, China.
Baile
B
Ning
The Guangzhou University of Chinese Medicine, Guangzhou, China.
Minzhen
M
Deng
The Guangzhou University of Chinese Medicine, Guangzhou, China.
Qinxin
Q
Zhang
The Guangzhou University of Chinese Medicine, Guangzhou, China.
Nanbu
N
Wang
The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 16, Jichang Road, Guangzhou, 510405, China. fangyq2@163.com.
Yongqi
Y
Fang
D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000494:pharmacology* / Q000627:therapeutic use; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000502:physiology*; D005260:Female; D008297:Male; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016627:Oxidopamine / Q000633:toxicity*; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D000071717:X-Box Binding Protein 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D008565:Membrane Proteins; D000071717:X-Box Binding Protein 1; C000605545:Xbp1 protein, rat; C012195:asarone; D016627:Oxidopamine; C486557:Ern2 protein, rat; D017346:Protein Serine-Threonine Kinases
Endoplasmic reticulum stress; IRE1; Parkinson’s disease; XBP1; β-Asarone