Corticibacteriumpopuli gen. nov., sp. nov., a member of the family Phyllobacteriaceae, isolated from bark of Populus×euramericana.
Two Gram-staining-negative, aerobic, motile, slimy, glossy bacterial strains were isolated from bark tissue of Populus×euramericana. The bacteria grew at 10-37 °C, pH 5-10, with optimal growth at 28-30 °C, pH 6.0-8.0. Both strains grew with 0-3 % (w/v) NaCl. In the maximum-likelihood phylogenetic tree, the two isolates formed a distinct branch within the family Phyllobacteriaceae, and they were not closely related to any of the genera within the family Phyllobacteriaceae. The two novel isolates werepositive for oxidase andcatalase activity. The polar lipids profile revealed diphosphatidylglycerol, phosphatidylcholine, phospholipid, phosphatidylethanolamine, phosphatidylglycerol and five unknown lipids. The major fatty acids were C18 : 1ω7c and C16 : 0. The DNA G+C content was 56.4 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, the two strains represent a novel species belonging to a novel genus of the family Phyllobacteriaceae, for which the name Corticibacterium populi gen. nov., sp. nov. is proposed. The type strain of the type species is 16B10-2-7T(=CFCC 12884T=KCTC 42249T).
Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Yong
Y
Li
Puyang Academy of Forestry, Puyang 457000, PR China.
Li-Min
LM
Guo
Puyang Academy of Forestry, Puyang 457000, PR China.
Ju-Pu
JP
Chang
Puyang Academy of Forestry, Puyang 457000, PR China.
Shou-Jiang
SJ
Xie
Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Chun-Gen
CG
Piao
Patent Examination Cooperation Center of the Patent Office, SIPO, Beijing, Beijing 100086, PR China.
Xia
X
Li
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
Corticibacterium populi; Phyllobacteriaceae; Proteobacteria