Structural characterization of low molecular weight polysaccharide from Astragalus membranaceus and its immunologic enhancement in recombinant protein vaccine against systemic candidiasis.
Structure and immunologic enhancement of low molecular weight polysaccharide (LMW-ASP) isolated from the root of Astragalus membranaceus (Fisch) Bge. Were detected in recombinant protein vaccine. Structure analysis of LMW-ASP revealed that LMW-ASP (Mw=5.6kDa) was an acid heteropolysaccharide, which consisted of Glc, Gal, Ara, Xyl and GalA in ratio of 10.0：1.3：1.7：1.0：0.9. Recombinant protein (rP-HSP90C) contained epitope C (LKVIRK) from heat shock protein 90 (HSP90) of Candida albicans was used as a vaccine. The results indicated that LMW-ASP significantly promoted specific antibody titers IgG, IgG1, IgG2b, and IL-2, IL-4, IL-10, IL-12 in sera of mice immunized with rP-HSP90C (p<0.05). It was also found LMW-ASP improved DTH response in HSP90C-injceted mice. More importantly, the mice immunized with rP-HSP90C/LMW-ASP had fewer CFU (colony forming unites) in the kidneys compared to the mice immunized with rP-HSP90C (p<0.05). Therefore, LMW-ASP could be exploited into the novel adjuvant to enhance the efficacy of recombinant protein vaccine.
School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
Fan
F
Yang
School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
Chunyu
C
Xiao
School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China.
Jing
J
Qu
School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, People's Republic of China. Electronic address: wanggy737@nenu.edu.cn.
Guiyun
G
Wang
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D027885:Astragalus propinquus; D002177:Candidiasis / Q000517:prevention & control; D016207:Cytokines / Q000097:blood; D000939:Epitopes / Q000494:pharmacology*; D005656:Fungal Proteins / Q000276:immunology*; D005657:Fungal Vaccines / Q000737:chemistry / Q000494:pharmacology*; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000517:prevention & control; D007074:Immunoglobulin G / Q000097:blood; D007668:Kidney / Q000187:drug effects; D008297:Male; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000908:Antibodies, Fungal; D016207:Cytokines; D000939:Epitopes; D005656:Fungal Proteins; D005657:Fungal Vaccines; D018841:HSP90 Heat-Shock Proteins; D007074:Immunoglobulin G; D011134:Polysaccharides; D011994:Recombinant Proteins
Adjuvant; Astragalus membranaceu; Candida albicans; Polysaccharide; Recombinant protein; Vaccine