Rapid Identification and Assignation of the Active Ingredients in Fufang Banbianlian Injection Using HPLC-DAD-ESI-IT-TOF-MS.
Fufang Banbianlian Injection (FBI) is a well-known traditional Chinese medicine formula composed of three herbal medicines. However, the systematic investigation on its chemical components has not been reported yet. In this study, a high-performance liquid chromatography combined with diode-array detector, and coupled to an electrospray ionization with ion-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method, was established for the identification of chemical profile in FBI. Sixty-six major constituents (14 phenolic acids, 14 iridoids, 20 flavonoids, 2 benzylideneacetone compounds, 3 phenylethanoid glycosides, 1 coumarin, 1 lignan, 3 nucleosides, 1 amino acids, 1 monosaccharides, 2 oligosaccharides, 3 alduronic acids and citric acid) were identified or tentatively characterized by comparing their retention times and MS spectra with those of standards or literature data. Finally, all constituents were further assigned in the individual herbs (InHs), although some of them were from multiple InHs. As a result, 11 compounds were from Lobelia chinensis Lour, 33 compounds were from Scutellaria barbata D. Don and 38 compounds were from Hedyotis diffusa Willd. In conclusion, the developed HPLC-DAD-ESI-IT-TOF-MS method is a rapid and efficient technique for analysis of FBI sample, and could be a valuable method for the further study on the quality control of the FBI.
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, PR China.
Sensen
S
Li
Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Zongtao
Z
Lin
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, PR China.
Haixiu
H
Jiang
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, PR China.
Lingkun
L
Tong
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, PR China.
Hong
H
Wang
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, PR China chenshizhong66@163.com.
Shizhong
S
Chen
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000145:classification / Q000302:isolation & purification*; D006027:Glycosides / Q000145:classification / Q000302:isolation & purification; D032064:Hedyotis / Q000737:chemistry*; D006801:Humans; D062385:Hydroxybenzoates / Q000302:isolation & purification; D039823:Iridoids / Q000145:classification / Q000302:isolation & purification; D029746:Lobelia / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D009005:Monosaccharides / Q000145:classification / Q000302:isolation & purification; D009844:Oligosaccharides / Q000145:classification / Q000302:isolation & purification; D031363:Scutellaria / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods / Q000592:standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000592:standards
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D062385:Hydroxybenzoates; D039823:Iridoids; D009005:Monosaccharides; D009844:Oligosaccharides; C017616:phenolic acid