Effect of root-extracts of Ficus benghalensis (Banyan) in pain in animal models.
BACKGROUND
According to the WHO, 70-80% population in developing countries still relies on nonconventional medicine mainly of herbal origin. Even in developed countries, use of herbal medicine is growing each year. Pain is an unpleasant feeling often caused by intense or damaging stimuli. Traditionally, different plant parts of Ficus benghalensis are claimed to have several analgesic properties. Few scientific evidences support these uses. Interestingly, still others contradict these uses. It was shocking to find very scarce scientific studies trying to solve the mystery.


METHODS
It was a quantitative experimental study in Swiss albino mice of either sex. Sample size was calculated using free sample size calculating software G*Power version 3.1.9.2. Hot-plate test and tail-flick test were central antinociceptive paradigms. Writhing test was peripheral model for pain. Test drugs were aqueous root extracts of F. benghalensis at 100 mg/kg and 200 mg/kg mouse weight prepared by Soxhlet method. Suitable negative and positive controls were used. The experimental results were represented as mean ± standard deviation statistical level of significance was set at P < 0.05. For calculation, parametric test - one-way analysis of variance (ANOVA) or nonparametric test - Mann-Whitney U-test was appropriately used.


RESULTS
Hot-plate reaction time at 100 mg/kg (13.64 ± 1.30 s) and 200 mg/kg (10.32 ± 2.23 s) were nonsignificant (P = 0.425 and P = 0.498, respectively) compared to negative control (11.87 ± 1.92 s). One-way ANOVA revealed nonsignificant (P = 0.178) between-group comparison in mean tail-flick reaction time. Test drug at 200 mg/kg produced statistically significant more writhing (36.00 ± 14.85 in 10 min) than negative control, normal saline (11.83 ± 12.43 in 10 min) or the positive control, Indomethacin (3.50 ± 5.21 in 10 min), P value being 0.031 and 0.003, respectively.


CONCLUSIONS
Aqueous root extracts of F. benghalensis at 200 mg/kg produces statistically significant writhing.
Department of Clinical Pharmacology and Therapeutics, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.
Dipesh Raj
DR
Panday
Department of Clinical Pharmacology and Therapeutics, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.
Gajendra Prasad
GP
Rauniar
Analgesic; Ficus; animal models; pain