Immobilization of Rubia tinctorum L. Suspension Cultures and Biomass Production.
Plants are natural sources of valuable secondary metabolites used as pharmaceuticals, agrochemicals, flavors, fragrances, colors, biopesticides, and food additives. There is an increasing demand to obtain these metabolites through more productive plant tissue applications and cell culture methods due to the importance of secondary metabolites.Immobilization of plant cells is a method used in plant cell cultures to induce secondary metabolite production. In this method, plant cells are fixed in or on a supporting material or matrix such as agar, agarose, calcium alginate, glass, or polyurethane foam. In the present study, three natural lignocellulosic materials, loofah sponge, and the long fibers of sisal and jute, were used to immobilize suspended R. tinctorum cells.
Department of Biomedical Engineering, Çorlu Faculty of Engineering, Namık Kemal University, 59860, Çorlu, Tekirdağ, Turkey. pinarnartop@gmail.com.
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Nartop
D018533:Biomass; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000166:cytology* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008031:Lignin / Q000737:chemistry; D059828:Plant Cells / Q000378:metabolism; D045470:Rubia / Q000166:cytology* / Q000378:metabolism; D064210:Secondary Metabolism
C036909:lignocellulose; D008031:Lignin
Biomass; Immobilization; Jute; Lignocellulosic matrices; Loofah; Plant cells; Secondary metabolite production; Sisal; Suspension culture