Reductive dehalogenation activity of indigenous microorganism in sediments of the Hackensack River, New Jersey.
Organohalogen pollutants are of concern in many river and estuarine environments, such as the New York-New Jersey Harbor estuary and its tributaries. The Hackensack River is contaminated with various metals, hydrocarbons and halogenated organics, including polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins. In order to examine the potential for microbial reductive dechlorination by indigenous microorganisms, sediment samples were collected from five different estuarine locations along the Hackensack River. Hexachlorobenzene (HCB), hexabromobenzene (HBB), and pentachloroaniline (PCA) were selected as model organohalogen pollutants to assess anaerobic dehalogenating potential. Dechlorinating activity of HCB and PCA was observed in sediment microcosms for all sampling sites. HCB was dechlorinated via pentachlorobenzene (PeCB) and trichlorobenzene (TriCB) to dichlorobenzene (DCB). PCA was dechlorinated via tetrachloroaniline (TeCA), trichloroanilines (TriCA), and dichloroanilines (DCA) to monochloroaniline (MCA). No HBB debromination was observed over 12 months of incubation. However, with HCB as a co-substrate slow HBB debromination was observed with production of tetrabromobenzene (TeBB) and tribromobenzene (TriBB). Chloroflexi specific 16S rRNA gene PCR-DGGE followed by sequence analysis detected Dehalococcoides species in sediments of the freshwater location, but not in the estuarine site. Analysis targeting 12 putative reductive dehalogenase (rdh) genes showed that these were enriched concomitant with HCB or PCA dechlorination in freshwater sediment microcosms.
Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, 76 Lipman Drive, New Brunswick, NJ 08901, USA. Electronic address: seoyean@scarletmail.rutgers.edu.
Seo Yean
SY
Sohn
Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, 76 Lipman Drive, New Brunswick, NJ 08901, USA. Electronic address: haggblom@AESOP.Rutgers.edu.
Max M
MM
Häggblom
D000814:Aniline Compounds / Q000032:analysis; D001969:Bromobenzenes / Q000032:analysis; D041862:Chloroflexi / Q000235:genetics / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D054879:Halogenation; D006581:Hexachlorobenzene / Q000032:analysis; D009515:New Jersey; D045483:Rivers / Q000737:chemistry*
D000814:Aniline Compounds; D001969:Bromobenzenes; D006581:Hexachlorobenzene; C017363:hexabromobenzene; C027673:2,3,4,5,6-pentachloroaniline
Dehalococcoides; Halogenated aromatics; Microbial reductive dehalogenation; Reductive dehalogenase; River sediments