Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.
The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach. We selected putative targets using the criteria of reduced expression upon zygotic Wnt knockdown, β-catenin binding within 50kb of the gene, and expression in tissues that receive Wnt signaling. Using these criteria, we found 21 novel direct transcriptional targets of Wnt/β-catenin signaling during gastrulation and in addition have identified putative regulatory elements for further characterization in future studies.
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Rachel A S
RAS
Kjolby
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Electronic address: harland@berkeley.edu.
Richard M
RM
Harland
D000818:Animals; D001665:Binding Sites; D001757:Blastomeres / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005775:Gastrula / Q000378:metabolism*; D054262:Gastrulation / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000502:physiology; D008648:Mesoderm / Q000378:metabolism; D009432:Neural Crest / Q000378:metabolism; D054259:Neural Tube / Q000378:metabolism; D009842:Oligopeptides; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D014158:Transcription, Genetic; D060449:Wnt Signaling Pathway; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics; D051176:beta Catenin / Q000378:metabolism
C495266:CTNNB1 protein, Xenopus; D036341:Intercellular Signaling Peptides and Proteins; D009842:Oligopeptides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D029867:Xenopus Proteins; D051176:beta Catenin; C110182:dkk1 protein, Xenopus; C085706:FLAG peptide
Mesoderm patterning; Neural crest induction; Posterior neural tube development; Transcriptional targets; Wnt/β-catenin signaling; Xenopus gastrulation