Blood Cytokine Profiles Associated with Distinct Patterns of Bronchopulmonary Dysplasia among Extremely Low Birth Weight Infants.
To explore differences in blood cytokine profiles among distinct bronchopulmonary dysplasia (BPD) patterns.



We evaluated blood spots collected from 943 infants born at ≤1000 g and surviving to 28 days on postnatal days 1, 3, 7, 14, and 21 for 25 cytokines. Infants were assigned to the following lung disease patterns: (1) no lung disease (NLD); (2) respiratory distress syndrome without BPD; (3) classic BPD (persistent exposure to supplemental oxygen until 28 days of age); or (4) atypical BPD (period without supplemental oxygen before 28 days). Median cytokine levels for infants with BPD were compared with the IQR of results among infants with NLD.



The distribution of enrolled infants by group was as follows: 69 (NLD), 73 (respiratory distress syndrome), 381 (classic BPD), and 160 (atypical BPD). The remaining 260 infants could not be classified because of missing data (104) or not fitting a predefined pattern (156). Median levels of 3 cytokines (elevated interleukin [IL]-8, matrix metalloproteinase-9; decreased granulocyte macrophage colony-stimulating factor) fell outside the IQR for at least 2 time points in both infants with atypical and classic BPD. Profiles of 7 cytokines (IL-6, IL-10, IL-18, macrophage inflammatory protein-1α, C-reactive protein, brain-derived neurotrophic factor, regulated on activation, normal T cell expressed and secreted) differed between infants with classic and atypical BPD.



Blood cytokine profiles may differ between infants developing classic and atypical BPD. These dissimilarities suggest the possibility that differing mechanisms could explain the varied patterns of pathophysiology of lung disease in extremely premature infants.
Strong Children's Research Center, University of Rochester School of Medicine and Dentistry, Rochester, NY. Electronic address: carl_dangio@urmc.rochester.edu.
Carl T
CT
D'Angio
Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL.
Namasivayam
N
Ambalavanan
Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL.
Waldemar A
WA
Carlo
Statistics and Epidemiology Unit, RTI International, Research Triangle Park, NC.
Scott A
SA
McDonald
Danish Centre for Neonatal Screening, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark.
Kristin
K
Skogstrand
Danish Centre for Neonatal Screening, Department of Clinical Biochemistry, Immunology and Genetics, Statens Serum Institut, Copenhagen, Denmark.
David M
DM
Hougaard
Department of Pediatrics, Wayne State University, Detroit, MI.
Seetha
S
Shankaran
Department of Pediatrics, Duke University, Durham, NC.
Ronald N
RN
Goldberg
Department of Pediatrics, Yale University School of Medicine, New Haven, CT.
Richard A
RA
Ehrenkranz
Department of Pediatrics, University of Texas Medical School at Houston, Houston, TX.
Jon E
JE
Tyson
Department of Pediatrics, Emory University School of Medicine, Children's Healthcare of Atlanta, Atlanta, GA.
Barbara J
BJ
Stoll
Statistics and Epidemiology Unit, RTI International, Rockville, MD.
Abhik
A
Das
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD.
Rosemary D
RD
Higgins
D001997:Bronchopulmonary Dysplasia / Q000097:blood* / Q000209:etiology / Q000503:physiopathology; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D052577:Infant, Extremely Low Birth Weight; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D010102:Oxygen Inhalation Therapy; D012127:Respiratory Distress Syndrome, Newborn / Q000097:blood* / Q000209:etiology / Q000503:physiopathology
D016207:Cytokines
chronic lung disease; inflammatory markers; premature infant; respiratory distress syndrome