Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol.
2-(4-Hydroxyphenyl)ehyl-β-D-glucopyranoside (salidroside) and 4-(2-hydroxyethyl)phenol (p-tyrosol) are famous food and medicine additives originally derived from alpine Rhodiola plants. Salidroside or p-tyrosol production by the endophytic fungus Rac56 (Phialocephala fortinii) was confirmed by UPLC/Q-TOF-MS and ¹H-NMR. The fermentation conditions were optimized by orthogonal design using data processing system software. The broth fermentation results showed that salidroside and p-tyrosol extraction yields from Rac56 were stable and reached 1.729 ± 0.06 mg and 1.990 ± 0.05 mg per mL of culture medium, respectively. The optimal conditions for salidroside and p-tyrosol production in fermentation culture of Rac56 were determined to be 25 °C, pH values of 7 and 5, Czapek-Dox culture medium volumes of 150 mL and 50 mL in 250 mL flasks, rotation speeds of 100× g and 200× g, and fermentation durations of 7 and 15 days, respectively. Under these optimal conditions, stable yields of 2.339 ± 0.1093 mg and 2.002 ± 0.0009 mg per mL of culture medium of salidroside and p-tyrosol, respectively, were obtained, indicating that the P. fortinii Rac56 strain is a promising source of these compounds.
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. CJL717@sxu.edu.cn.
Jinlong
J
Cui
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. guott880118@163.com.
Tingting
T
Guo
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. chao@sxu.edu.cn.
Jianbin
J
Chao
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. mlwang@sxu.edu.cn.
Mengliang
M
Wang
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China. wangjunh@sxu.edu.cn.
Junhong
J
Wang
D060026:Endophytes / Q000737:chemistry / Q000254:growth & development; D005285:Fermentation; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D010802:Phylogeny; D029771:Rhodiola / Q000382:microbiology*; D004718:Saccharomycetales / Q000737:chemistry / Q000254:growth & development*; D013696:Temperature
D005960:Glucosides; D010636:Phenols; C011867:4-hydroxyphenylethanol; C009172:rhodioloside; D010626:Phenylethyl Alcohol
Phialocephala fortinii; Rhodiola angusta; accumulation of active component; fungal bioactivity