Assessment of In Vitro Biological Activities of Anthocyanins-Rich Plant Species Based on Plinia cauliflora Study Model.
Plinia cauliflora (jaboticaba) is a native fruit tree from Brazilian rainforest widely used in popular medicine to prevent diarrhea, asthma, and infections. Studies have shown that the major therapeutic potential of jaboticaba fruits is on its peel, a rich source of anthocyanins. These secondary metabolites have well-known antioxidant and anti-inflammatory activities and have been claimed to be effective to treat diabetes, cancer, cardiovascular diseases, and stroke. This chapter describes a series of methodologies to evaluate important in vitro biological activities like cytotoxicity, proliferation, and migration of a hydroalcoholic extract of jaboticaba peel on mouse fibroblast L929 line. Assays to assess total phenolic, flavonoid, and anthocyanin contents and antioxidant activities are described as well.
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.
Heloisa S
HS
Pitz
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.
Adriana C D
AC
Trevisan
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.
Fausto R
FR
Cardoso
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.
Aline
A
Pereira
Nanoscoping Solutions in Nanotechnology, 250, Vitor Lima Street, 88040-400, Florianópolis, Santa Catarina State, Brazil.
Eduardo L G
EL
Moreira
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.|Nanoscoping Solutions in Nanotechnology, 250, Vitor Lima Street, 88040-400, Florianópolis, Santa Catarina State, Brazil.
Manuel A
MA
de Prá
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.|Nanoscoping Solutions in Nanotechnology, 250, Vitor Lima Street, 88040-400, Florianópolis, Santa Catarina State, Brazil.
Letícia
L
Mazzarino
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.|Nanoscoping Solutions in Nanotechnology, 250, Vitor Lima Street, 88040-400, Florianópolis, Santa Catarina State, Brazil.
Maria B
MB
Veleirinho
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.
Rosendo A
RA
Yunes
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil.
Rosa M R
RM
do Valle
Plant Morphogenesis and Biochemistry Laboratory, Natural Products Core, Federal University of Santa Catarina, 1346, 401 Road., PO Box 476, 88040-900, Florianópolis, Santa Catarina State, Brazil. m.maraschin@ufsc.br.
Marcelo
M
Maraschin
D000818:Animals; D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D027822:Myrtaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
Anthocyanins; Biological activities; DPPH assay; EdU assay; MTT assay; Phenolic compounds; Phytochemistry; Plinia cauliflora; Scratch assay; Total flavonoids