Nitric Oxide Measurement from Purified Enzymes and Estimation of Scavenging Activity by Gas Phase Chemiluminescence Method.
In plants, nitrate reductase (NR) is a key enzyme that produces nitric oxide (NO) using nitrite as a substrate. Lower plants such as algae are shown to have nitric oxide synthase enzyme and higher plants contain NOS activity but enzyme responsible for NO production in higher plants is subjected to debate. In plant nitric oxide research, it is very important to measure NO very precisely in order to determine its functional role. A significant amount of NO is being scavenged by various cell components. The net NO production depends in production minus scavenging. Here, we describe methods to measure NO from purified NR and inducible nitric oxide synthase from mouse (iNOS), we also describe a method of measure NO scavenging by tobacco cell suspensions and mitochondria from roots.
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, 110067, New Delhi, India.
Aprajita
A
Kumari
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, 110067, New Delhi, India.
Alok Kumar
AK
Gupta
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, 110067, New Delhi, India.
Sonal
S
Mishra
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, 110067, New Delhi, India.
Aakanksha
A
Wany
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, 110067, New Delhi, India. jgk@nipgr.ac.in.
Kapuganti Jagadis
KJ
Gupta
D004798:Enzymes / Q000378:metabolism*; D005740:Gases; D049449:Luminescence; D009569:Nitric Oxide / Q000378:metabolism*
D004798:Enzymes; D005740:Gases; D009569:Nitric Oxide
Chemiluminescence; Mitochondria; Nitrate reductase; Nitric oxide synthase; Scavenging