Start-up phase of an anaerobic full-scale farm reactor - Appearance of mesophilic anaerobic conditions and establishment of the methanogenic microbial community.
The goal of this study was to investigate how the microbial community structure establishes during the start-up phase of a full-scale farm anaerobic reactor inoculated with stale and cold cattle slurry. The 16S/18S high-throughput amplicon sequencing results showed an increase of the bacterial, archaeal and eukaryotic diversity, evenness and richness during the settlement of the mesophilic anaerobic conditions. When a steady performing digestion process was reached, the microbial diversity, evenness and richness decreased, indicating the establishment of a few dominant microbial populations, best adapted to biogas production. Interestingly, among the environmental parameters, the temperature, alkalinity, free-NH3, total solids and O2 content were found to be the main drivers of microbial dynamics. Interactions between eukaryotes, characterized by a high number of unknown organisms, and the bacterial and archaeal communities were also evidenced, suggesting that eukaryotes might play important roles in the anaerobic digestion process.
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41 rue du Brill, L-4422 Belvaux, Luxembourg; Laboratoire Sols et Environnement, Université de Lorraine, UMR 1120, 2 avenue de la Forêt de Haye, TSA 40602, F-54518 Vandœuvre-lès-Nancy, France; INRA, Laboratoire Sols et Environnement, UMR 1120, 2 avenue de la Forêt de Haye, TSA 40602, F-54518 Vandœuvre-lès-Nancy, France.
Xavier
X
Goux
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41 rue du Brill, L-4422 Belvaux, Luxembourg.
Magdalena
M
Calusinska
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41 rue du Brill, L-4422 Belvaux, Luxembourg.
Marie
M
Fossépré
Laboratoire Sols et Environnement, Université de Lorraine, UMR 1120, 2 avenue de la Forêt de Haye, TSA 40602, F-54518 Vandœuvre-lès-Nancy, France; INRA, Laboratoire Sols et Environnement, UMR 1120, 2 avenue de la Forêt de Haye, TSA 40602, F-54518 Vandœuvre-lès-Nancy, France.
Emile
E
Benizri
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41 rue du Brill, L-4422 Belvaux, Luxembourg. Electronic address: philippe.delfosse@list.lu.
Philippe
P
Delfosse
D000693:Anaerobiosis; D000818:Animals; D001105:Archaea / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D002417:Cattle; D019605:Euryarchaeota / Q000145:classification / Q000235:genetics / Q000502:physiology; D000072480:Farms; D008372:Manure / Q000382:microbiology; D008697:Methane / Q000378:metabolism; D018505:Waste Management / Q000379:methods
D056804:Biofuels; D008372:Manure; D008697:Methane
16S/18S high-throughput amplicon sequencing; Anaerobic digestion; Archaea; Bacteria; Eukaryotes; Microbial ecology