A novel mast cell co-culture microfluidic chip for the electrochemical evaluation of food allergen.
In this study a novel cell-to-cell electrochemical microfluidic chip was developed for qualitative and quantitative analysis of food allergen. Microfluidic cell culture, food allergen-induced cell morphological changes, and cell metabolism measurements were performed simultaneously using the aforementioned device. RBL-2H3 mast cells and ANA-1 macrophages have been used within a cell co-culture model to observe their allergic response when they are introduced to the antigen stimulus. Two cell cultivation microfluidic channels are located in the microfluidic chip, which is fabricated with four groups of gold electrodes, with an additional "capillary". In order to detect the allergic response, the cells were stimulated with dinitrophenylated bovine serum albumin (DNP-BSA) without anti-DNP IgE incubation. When exocytosis occurs, the cell-secreted inflammatory cytokines were measured by enzyme-linked immuno sorbent assay (ELISA) and cell impedance changes were detected using cell-based electrochemical assay. Results indicate that the real-time cell allergic response are accurately monitored by this electrochemical microfluidic chip, which provides a general example of rapidly prototyped low-cost biosensor technology for applications in both food allergen detection and investigation.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Hui
H
Jiang
School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, PR China.
Donglei
D
Jiang
State Key Laboratory of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, PR China.
Pei
P
Zhu
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Fuwei
F
Pi
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Jian
J
Ji
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Chao
C
Sun
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Jiadi
J
Sun
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: sxlzzz@jiangnan.edu.cn.
Xiulan
X
Sun
D000485:Allergens / Q000276:immunology*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D002450:Cell Communication; D002460:Cell Line; D018920:Coculture Techniques / Q000295:instrumentation*; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000295:instrumentation; D005512:Food Hypersensitivity / Q000276:immunology*; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D051381:Rats
D000485:Allergens
Electrochemical microfluidic chip; Impedance; Macrophages; Mast cells