Small-Protein-Stabilized Semiconductor Nanoprobe for Targeted Imaging of Cancer Cells.
Recently, semiconductor nanoparticles such as quantum dots (QDs) have attracted significant attention for bioimaging. Complex chemical functionalization, surface modification, and bioconjugation chemistry are generally required to tag biomolecules to QDs for imaging of different biomarkers. In this study, we report a simple method for production of QDs stabilized by the small protein, Affibody (AF-QDs) for fluorescent imaging of the human epidermal growth factor receptor type 2 (HER2) in human A549 lung cancer cells. This one-pot synthesis of AF-QDs avoids complex chemical conjugation procedures and demonstrates a promising approach for the preparation of fluorescent nanoprobes for imaging of cancer targets.
Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, 195 Chuangxin Road, Shenyang, 110000, China.
Ning
N
Zhao
Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, 195 Chuangxin Road, Shenyang, 110000, China.
Siyu
S
Liu
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.
Qike
Q
Jiang
Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, 195 Chuangxin Road, Shenyang, 110000, China.
Tian
T
Lan
Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 1201 Welch Road, Stanford, CA, 94040, USA. zcheng@stanford.edu.
Zhen
Z
Cheng
Institute of Molecular Medicine, College of Life and Health Sciences, Northeastern University, 195 Chuangxin Road, Shenyang, 110000, China. simonliu@mail.neu.edu.cn.
Hongguang
H
Liu
D000072283:A549 Cells; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D009369:Neoplasms / Q000000981:diagnostic imaging*; D010316:Particle Size; D045663:Quantum Dots / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*
D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C000622617:Cys-ZHER2-342 affibody; C508053:ERBB2 protein, human
Affibody; HER2; fluorescent imaging; one-pot synthesis; quantum dots