Micropropagation, Acclimatization, and Greenhouse Culture of Veratrum californicum.
Micropropagation and production of Veratrum californicum is most successful when using a premixed Murishage and Skoog basal medium with vitamins and a 5-week subculture cycle at 16 °C for multiplication. These culture conditions provide the best percent survival after acclimatization in the greenhouse. However, clone response to temperature and light quality within culture conditions varies. Micropropagated plants have mass and morphology similar to 2- or 3-year-old seedlings. Acclimatized plantlets can then be grown in the greenhouse using sub-irrigation (ebb and flood) to maintain substrate volumetric water content > 44 %. Growth cycle in the greenhouse must be about 100 days, followed by dormancy for 5 months at 5 °C.
Department of Agricultural and Environmental Sciences, Clemson University, E-143 Poole Agricultural Center, Clemson, SC, 29634, USA. swhite4@clemson.edu.
Sarah A
SA
White
Department of Agricultural and Environmental Sciences, Clemson University, E-143 Poole Agricultural Center, Clemson, SC, 29634, USA.
Jeffrey
J
Adelberg
Department of Agricultural and Environmental Sciences, Clemson University, E-143 Poole Agricultural Center, Clemson, SC, 29634, USA.
Jacqueline
J
Naylor-Adelberg
Infinity Pharmaceutical, 780 Memorial Drive, Cambridge, MA, 02139, USA.
David A
DA
Mann
Department of Agricultural and Environmental Sciences, Clemson University, E-143 Poole Agricultural Center, Clemson, SC, 29634, USA.
Ju Yeon
JY
Song
Department of Agricultural and Environmental Sciences, Clemson University, E-143 Poole Agricultural Center, Clemson, SC, 29634, USA.
Youping
Y
Sun
D000064:Acclimatization; D000383:Agriculture / Q000379:methods*; D003080:Cold Temperature; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D057445:Plant Dormancy; D014703:Veratrum / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology
D003470:Culture Media
Chilling; Cyclopamine; Dormancy; Ebb-and-flood irrigation; Low-temperature propagation; Veratrum californicum