First evidence for truffle production from plants inoculated with mycelial pure cultures.
Truffle (Tuber spp.) cultivation is based on raising mycorrhizal trees in greenhouses that have been inoculated with suspensions of ascospores. The problem with this is that pests, pathogens, and other mycorrhizal fungi can contaminate the trees. Furthermore, because ascospores are produced sexually, each plant potentially has a different genetic mycorrhizal makeup from each other so tailoring the mycorrhizal component of plants to suit a particular set of soil and climatic conditions is out of the question. Here, we report on the production of Tuber borchii-mycorrhized plants using pure cultures, establishing a truffière with these and subsequent production of its fruiting bodies. This study opens up the possibility of producing commercial numbers of Tuber-mycorrhized trees for truffle cultivation using mycelial inoculation techniques. It also poses questions about the mechanism of fertilization between the different strains which were located in different parts of the experimental truffière.
Department of Life, Health and Environmental Sciences, University of L'Aquila, Via Vetoio, Coppito 1, L'Aquila, 67100, Italy.
Mirco
M
Iotti
Department of Agricultural Sciences, Bologna University, Viale Fanin 46, 40127, Bologna, Italy.
Federica
F
Piattoni
Department of Agricultural Sciences, Bologna University, Viale Fanin 46, 40127, Bologna, Italy.
Pamela
P
Leonardi
Truffles and Mushrooms (Consulting) Ltd, P.O. Box 268, Dunedin, 9054, New Zealand.
Ian R
IR
Hall
Department of Agricultural Sciences, Bologna University, Viale Fanin 46, 40127, Bologna, Italy. alessandr.zambonelli@unibo.it.
Alessandra
A
Zambonelli
D001203:Ascomycota / Q000145:classification / Q000502:physiology*; D031211:Corylus / Q000382:microbiology*; D025282:Mycelium / Q000502:physiology*; D038821:Mycorrhizae / Q000502:physiology*; D028223:Pinus / Q000382:microbiology*; D029963:Quercus / Q000382:microbiology*; D036226:Seedlings
Ascoma production; Mycelial inoculation; Strains; Tuber borchii