Study of strains of Candida spp. Isolated from catheters in UHC of Oran (Algeria): Identification and antifungal susceptibility.
OBJECTIVE
The increasing incidence of Candida spp., and the vital prognosis often compromise for patients with Candida species make urgent the exact knowledge of their distribution worldwide and exhaust action antifungals currently used in clinical. That why we carry out an epidemiological study of Candida species and testing their susceptibility against two antifungals: amphotericin B and caspofungin.


METHODS
Samplings of peripheral venous catheters (PVC) were carried out from during 8months on the services of Internal medicine, Surgery A and Neonatology of Oran's University Hospital Center (UHC). The study of the susceptibility of Candida species to antifungal agents was performed according to the Clinical Laboratory Standards Institute (CLSI 2008).


RESULTS
From 300 samples, 25 yeasts were isolated. The rate of colonization PVC was 8.33% by Candida spp. The most isolated strains were Candida parapsilosis with 64% of cases, followed by Candida albicans (12%) then 8% for Candida glabrata and Candida krusei. However, only 4% of isolates were Candida famata or Candida lusitaniae. Furthermore all isolated strains were susceptible to amphotericin B with Minimum Inhibitory Concentrations (MIC) ranging from 0.25 to 1μg/mL. MIC obtained with caspofungin vary from 0.0625 to 2μg/mL for all strains. Moreover, one strain of C. krusei is resistant to caspofungin with a MIC superior to 8μg/mL.


CONCLUSIONS
All though caspofungin is at least as effective as amphotericin B, it is better tolerated for the treatment of invasive fungal infections.
Laboratory Antifungals Antibiotics Physico-Chemical, Synthesis and Biological Activity (LapSab), Tlemcen University, BP 119, 13000 Tlemcen, Algeria. Electronic address: doc.meri@hotmail.fr.
M
M
Bendjelloul
Laboratory Antifungals Antibiotics Physico-Chemical, Synthesis and Biological Activity (LapSab), Tlemcen University, BP 119, 13000 Tlemcen, Algeria. Electronic address: z_boucherit@yahoo.fr.
Z
Z
Boucherit-Otmani
Laboratory Antifungals Antibiotics Physico-Chemical, Synthesis and Biological Activity (LapSab), Tlemcen University, BP 119, 13000 Tlemcen, Algeria; University Center of Ain Temouchent, Ain Temouchent, Algeria. Electronic address: boucheritkebir@yahoo.fr.
K
K
Boucherit
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000462:Algeria / Q000453:epidemiology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D055499:Catheter-Related Infections / Q000453:epidemiology / Q000382:microbiology*; D057785:Catheters / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D025141:Drug Resistance, Fungal / Q000187:drug effects*; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D055815:Young Adult
D000935:Antifungal Agents
Amphotericin B; Amphotéricine B; Candida spp.; Caspofungin; Caspofungine; Catheters; Cathéters; Infections nosocomiales; Nosocomial infections