Putative anticancer potential of novel 4-thiazolidinone derivatives: cytotoxicity toward rat C6 glioma in vitro and correlation of general toxicity with the balance of free radical oxidation in rats.
OBJECTIVE
To evaluate the cytotoxic action of 4-thiazolidinone derivatives (ID 3288, ID 3882, and ID 3833) toward rat glioma C6 cells and to compare the effects of these compounds and doxorubicin on the balance of free radical oxidation (FRO) and antioxidant activity (AOA) in the serum of rats.


METHODS
Glioma cells were treated with ID 3882, ID 3288, ID 3833, and doxorubicin, and their cytotoxicity was studied using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Trypan blue exclusion test, light and fluorescent microscopy, and flow cytometric study of cell cycling and apoptosis, including measuring of Annexin V-positive cells. The contents of superoxide radical, hydrogen peroxide, hydroxyl radical, malonic dialdehyde, and hydrogen sulfide were measured in the serum of rats. Enzymatic activity of superoxide dismutase (SOD), catalase (Cat), and glutathione peroxydase (GPO) was determined.


RESULTS
Among novel 4-thiazolidinone derivatives, ID 3288 was most toxic toward rat glioma C6 cells, even compared with doxorubicin. All applied derivatives were less active than doxorubicin in inducing reactive oxygen species-related indicators in the serum of rats. A similar effect was observed when enzymatic indicators of AOA processes were measured. While doxorubicin inhibited the activity of SOD, GPO, and Cat, the effects of 4-thiazolidinone derivatives were less prominent.


CONCLUSIONS
Novel 4-thiazolidinone derivatives differ in their antineoplastic action toward rat glioma C6 cells, and ID 3288 possesses the highest activity compared to doxorubicin. Measurement of indicators of FRO and AOA in the serum of rats treated with these compounds showed their lower general toxicity compared with doxorubicin's toxicity.
Lesya I
LI
Коbylinska
Nataliya M
NM
Boiko
Rostyslav R
RR
Panchuk
Iryna I
II
Grytsyna
Olga Yu
OY
Klyuchivska
Liliya P
LP
Biletska
Roman B
RB
Lesyk
Borys S
BS
Zіmenkovsky
Rostyslav S. Stoika, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14/16, 79005, Lviv, Ukraine, stoika@cellbiol.lviv.ua.
Rostyslav S
RS
Stoika
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005910:Glioma / Q000188:drug therapy; D020128:Inhibitory Concentration 50; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D053778:Thiazolidines / Q000494:pharmacology*
C000614368:4-thiazolidinone; D000970:Antineoplastic Agents; D005609:Free Radicals; D017382:Reactive Oxygen Species; D053778:Thiazolidines; D004317:Doxorubicin; D013482:Superoxide Dismutase