In Silico Study to Develop a Lectin-Like Protein from Mushroom Agaricus bisporus for Pharmaceutical Application.
A lectin-like protein of unknown function designated as LSMT was recently discovered in the edible mushroom Agaricus bisporus. The protein shares high structural similarity to HA-33 from Clostridium botulinum (HA33) and Ricin-B-like lectin from the mushroom Clitocybe nebularis (CNL), which have been developed as drug carrier and anti-cancer, respectively. These homologous proteins display the ability to penetrate the intestinal epithelial cell monolayer, and are beneficial for oral administration. As the characteristics of LSMT are unknown, a structural study in silico was performed to assess its potential pharmaceutical application. The study suggested potential binding to target ligands such as HA-33 and CNL although the nature, specificity, capacity, mode, and strength may differ. Further molecular docking experiments suggest that interactions between the LSMT and tested ligands may take place. This finding indicates the possible use of the LSMT protein, initiating new research on its use for pharmaceutical purposes.
Dexa Laboratories of Biomolecular Sciences, Industri Selatan V, Blok PP No. 7, Kawasan Industri, Jababeka II, Cikarang 17550, Indonesia.
Wangsa Tirta
WT
Ismaya
Research group of Pharmaceutics, School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesa 10, Bandung 40132, Indonesia.
Yunita
Research group of Pharmacochemistry, School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesa 10, Bandung 40132, Indonesia.
Sophi
S
Damayanti
Dexa Laboratories of Biomolecular Sciences, Industri Selatan V, Blok PP No. 7, Kawasan Industri, Jababeka II, Cikarang 17550, Indonesia.
Caroline
C
Wijaya
Dexa Laboratories of Biomolecular Sciences, Industri Selatan V, Blok PP No. 7, Kawasan Industri, Jababeka II, Cikarang 17550, Indonesia.
Raymond R
RR
Tjandrawinata
Research group of Biotechnology, School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesa 10, Bandung 40132, Indonesia.
Debbie Sofie
DS
Retnoningrum
Research group of Pharmaceutics, School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesa 10, Bandung 40132, Indonesia.
Heni
H
Rachmawati
In silico protein study; Lectin-like protein; Light subunit mushroom tyrosinase; Molecular docking; Novel pharmaceutical protein; Oral delivery