Comparison of TGF-β1 and NO production by mesenchymal stem cells isolated from murine lung and adipose tissues.
BACKGROUND
Mesenchymal stem cells (MSCs) are cell sources for tissues regeneration. By secretion of soluble factors including transforming growth factor-β (TGF-β1) and nitric oxide (NO), MSCs are also able to regulate the immune system. MSCs have been disclosed in lung and adipose tissues with insufficient comparison between the tissues.


OBJECTIVE
In this study, specific differentiation and the expression of surface antigens as well as TGF-β1 and NO productive levels were compared in murine lung-derived MSCs (LMSCs) and adipose tissue-derived MSCs (ADMSCs).


METHODS
MSCs were isolated from murine lung and adipose tissues and cultured. Both cell populations were characterized using multilineage potential and the expression of surface antigenic proteins, CD73, CD105, CD34, CD45, and CD11b. Finally, levels of TGF-β1 and NO were evaluated and compared in ADMSCs and LMSCs.


RESULTS
Expression of CD73 and CD105; lack of the expression of CD34, CD45, and CD11b markers; as well as adipocyte and osteocyte differentiations were detected in both adult stem cells. No significant difference was found in TGF-β1 and NO production between two stem cell populations.


CONCLUSIONS
Our data showed that LMSCs and ADMSCs have comparable phenotype and TGF-β1 and NO production.
a Immunoregulation Research Center, Shahed University , Tehran , Iran ;
Zahra
Z
Hosseinpur
b Department of Immunology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran ;
Seyed Mahmoud
SM
Hashemi
c Department of Immunology, Faculty of Medical Sciences , Tehran University of Medical Sciences , Tehran , Iran.
Eisa
E
Salehi
a Immunoregulation Research Center, Shahed University , Tehran , Iran ;
Tooba
T
Ghazanfari
D000273:Adipose Tissue / Q000276:immunology*; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000276:immunology*; D008168:Lung / Q000276:immunology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000276:immunology*
D015703:Antigens, CD; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1; D009569:Nitric Oxide
Adipose tissue; TGF-β1; lung; mesenchymal stem cells; nitric oxide