Description of Comamonas sediminis sp. nov., isolated from lagoon sediments.
Strain S3Twas isolated from lagoon sediments, and appeared as transparent colonies on agar plates, with cells staining Gram-negative. Catalase and oxidase were positive. S3Thydrolyzed starch, casein and tween-20, while urea, chitin, gelatin and tween-80 were not hydrolysed. C18 : 1ω6c/C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c,C17 : 0 cyclo and C16 : 0 were the predominant fatty acids with minor amounts of C10 : 0 3-OH, C12 : 0, C14 : 0 and C16 : 0 2-OH. S3Tcontained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) as major polar lipids with minor amounts of unidentified phospholipid (PL) and unidentified lipids (L1-2). Genomic DNA G+C content was 68.3 mol%. 16S rRNA gene sequence comparisons indicated that S3Trepresents a member of the genus Comamonas in family Comamonadaceae of the class Betaproteobacteria. S3Thas a sequence similarity of 98.96 % with Comamonas koreensis YH12T, 97.93 % with Comamonas guangdongensis CY01Tand <96.97 % with other members of the genus Comamonas. DNA-DNA hybridization values between S3Tand the type strains of the most closely related species were clearly below the 70 % threshold. On the basis of phenotypic, genotypic and phylogenetic analysis, it is proposed that S3Trepresents a novel species of the genus Comamonas, for which the name Comamonas sediminis sp. nov. is proposed. The type strain is S3T(=KEMB 563-466T=JCM 31169T).
Department of Life Science, College of Natural Science, Kyonggi University, Iuidong Yeongtong-gu, Suwon, South Korea.
Y
Y
Subhash
Department of Environmental, Earth and Geospatial Science, North Carolina Central University, USA.
John J
JJ
Bang
Department of Biological Sciences, Campbell University, USA.
Taek H
TH
You
Department of Life Science, College of Natural Science, Kyonggi University, Iuidong Yeongtong-gu, Suwon, South Korea.
Sang-Seob
SS
Lee
D015373:Bacterial Typing Techniques; D001482:Base Composition; D020585:Comamonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D009657:North Carolina; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
Comamonadaceae; Comamonas sediminis; novel species