Immunoexpression of Cyclin D1 and PTEN in Various Endometrial Pathologies.
OBJECTIVE
To determine the expression of cyclin D1 and PTEN (phosphatase and tensin homolog) in endometrial hyperplasias and neoplasias.


METHODS
Analytical study.


METHODS
The study was conducted at BMSI, JPMC, Karachi, from January 2008 to December 2012.


METHODS
Analysis of endometrial samples, comprising of hysterectomies and curettage, was carried out. Immunohistochemical staining was done for PTEN and cyclin D1 expression.


RESULTS
Fifty-three endometrial samples including 23 endometrial carcinomas, 6 complex hyperplasias with atypia, 14 complex hyperplasias without atypia, 6 simple hyperplasias without atypia and 4 normal proliferative endometrium were analyzed. Fifty-two percent (12 out of 23) and 48% (11 out of 23) cases of endometrial carcinomas showed complete loss of PTEN expression and cyclin D1 over expression, respectively. Five (5 out of 6) cases of complex hyperplasias with atypia and 64.28% (9 out of 14) cases of complex hyperplasia without atypia showed complete loss of or diminished expression of PTEN whereas 66.66% (4 out of 6) cases of endometrial hyperplasia with atypia and 50% (7 out of 14) cases of endometrial hyperplasia without atypia showed cyclin D1 overexpression (p &amp;lt; 0.001).


CONCLUSIONS
Loss of PTEN, expression and cyclin D1 overexpression was seen in a significant number of well differentiated endometrial adenocarcinomas and complex hyperplasias with atypia, suggesting both as an early event in endometrial carcinogenesis.
Department of Pathology, Bahria University Medical and Dental College, Karachi.
Summayya
S
Shawana
Department of Pathology, Basic Medical Sciences Institute, JPMC, Karachi.
Shahnaz Imdad
SI
Kehar
Department of Pathology, Jinnah Medical and Dental College,Karachi.
Saleha
S
Masood
Department of Physiology, Jinnah Medical and Dental College,Karachi.
Iram
I
Aamir
D014408:Biomarkers, Tumor / Q000378:metabolism; D019938:Cyclin D1 / Q000032:analysis / Q000378:metabolism*; D004714:Endometrial Hyperplasia / Q000378:metabolism* / Q000473:pathology; D016889:Endometrial Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051059:PTEN Phosphohydrolase / Q000032:analysis / Q000378:metabolism*; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology
D014408:Biomarkers, Tumor; D019938:Cyclin D1; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human