Differential expression of extracellular matrix genes in glenohumeral capsule of shoulder instability patients.
Anterior shoulder instability is a common orthopedic problem. After a traumatic shoulder dislocation, patients present a plastic deformation of the capsule. The shoulder instability biology remains poorly understood. We evaluated the expression of genes that encode the cartilage oligomeric matrix protein (COMP), fibronectin 1 (FN1), tenascin C (TNC) and tenascin XB (TNXB) in the glenohumeral capsule of anterior shoulder instability patients and controls. Moreover, we investigated the associations between gene expression and clinical parameters. The gene expression was evaluated by quantitative reverse transcription-polymerase chain reaction in the antero-inferior (macroscopically injured region), antero-superior and posterior regions of the capsule of 29 patients with shoulder instability and 8 controls. COMP expression was reduced and FN1 and TNC expression was increased in the antero-inferior capsule region of cases compared to controls (p < 0.05). TNC expression was increased in the posterior capsule portion of shoulder instability patients (p = 0.022). COMP expression was reduced in the antero-inferior region compared to the posterior region of shoulder instability patients (p = 0.007). In the antero-inferior region, FN1 expression was increased in the capsule of patients with more than one year of symptoms (p = 0.003) and with recurrent dislocations (p = 0.004) compared with controls. FN1 and TNXB expression was correlated with the duration of symptoms in the posterior region (p < 0.05). Thus, COMP, FN1, TNC and TNXB expression was altered across the capsule of shoulder instability patients. Dislocation episodes modify FN1, TNC and TNXB expression in the injured tissue. COMP altered expression may be associated with capsule integrity after shoulder dislocation, particularly in the macroscopically injured portion.
a Departamento de Ortopedia e Traumatologia , Universidade Federal de São Paulo , São Paulo , Brazil.
Paulo Santoro
PS
Belangero
a Departamento de Ortopedia e Traumatologia , Universidade Federal de São Paulo , São Paulo , Brazil.|b Departamento de Morfologia e Genética , Universidade Federal de São Paulo , São Paulo , Brazil.
Mariana Ferreira
MF
Leal
b Departamento de Morfologia e Genética , Universidade Federal de São Paulo , São Paulo , Brazil.
Eduardo Antônio
EA
Figueiredo
a Departamento de Ortopedia e Traumatologia , Universidade Federal de São Paulo , São Paulo , Brazil.
Carina
C
Cohen
a Departamento de Ortopedia e Traumatologia , Universidade Federal de São Paulo , São Paulo , Brazil.
Carlos Vicente
CV
Andreoli
b Departamento de Morfologia e Genética , Universidade Federal de São Paulo , São Paulo , Brazil.
Marília Cardoso
MC
Smith
a Departamento de Ortopedia e Traumatologia , Universidade Federal de São Paulo , São Paulo , Brazil.
Alberto de Castro
Ade C
Pochini
a Departamento de Ortopedia e Traumatologia , Universidade Federal de São Paulo , São Paulo , Brazil.
Benno
B
Ejnisman
a Departamento de Ortopedia e Traumatologia , Universidade Federal de São Paulo , São Paulo , Brazil.
Moises
M
Cohen
D000328:Adult; D016022:Case-Control Studies; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D017746:Joint Capsule / Q000473:pathology*; D008297:Male; D011300:Preoperative Care; D012783:Shoulder Dislocation / Q000235:genetics* / Q000601:surgery; D012785:Shoulder Joint / Q000473:pathology*; D019063:Tenascin / Q000235:genetics / Q000378:metabolism
D016326:Extracellular Matrix Proteins; C067625:FN1 protein, human; D005353:Fibronectins; D019063:Tenascin; C081865:tenascin X; D003094:Collagen
Extracellular matrix; gene expression; glenohumeral capsule; glycoproteins; shoulder instability