Histologic and Histomorphometric Comparison of Bone Regeneration Between Bone Morphogenetic Protein-2 and Platelet-Derived Growth Factor-BB in Experimental Groups.
Efficacy of recombinant human bone morphogenetic protein-2 (rhBMP-2) and recombinant human platelet-derived growth factor-BB (rhPDGF-BB) delivered via absorbable collagen sponge (ACS) on bone formation was evaluated in guinea pig tibias. Three-millimeter-circular bone tibia defects were created in 24 guinea pigs assigned randomly to 4 groups according to the following defect filling materials: ACS only, rhBMP-2+ACS, rhPDGF-BB+ACS, or empty. New bone formation was evaluated histologically and histomorphometrically at 15 (early healing) and 45 days (late healing). Mean new bone per total defect area ratio was 0.73, 0.57, 0.43, and 0.42 in rhBMP-2+ACS, rhPDGF-BB+ACS, ACS only, and empty groups at early healing, respectively. During early healing, significantly more new bone formation was observed in rhBMP-2+ACS and rhPDGF-BB+ACS groups than in the control groups. New bone formation was significantly higher with rhBMP-2+ACS than with rhPDGF-BB+ACS. Mean new bone per total defect area ratio was 0.81, 0.86, 0.74, and 0.75 in the rhBMP-2+ACS, rhPDGF-BB+ACS, ACS only, and empty groups at late healing, respectively. During late healing, new bone formation was significantly higher in the rhPDGF-BB+ACS group relative to both control groups, but the results did not differ significantly from those in the rhBMP-2+ACS group. New bone formation in the rhBMP-2+ACS group did not change significantly between the healing periods. In the rhPDGF-BB+ACS group, however, new bone formation was significantly higher in the late healing period. Both growth factors accelerated new bone formation in the early healing period. Although rhBMP-2 was more effective in the early healing period, the effects of rhPDGF-BB were longer lasting.
*Department of Oral Implantology, Istanbul University Faculty of Dentistry†Department of Oral and Maxillofacial Surgery, Medipol University Faculty of Dentistry‡Department of Histology and Embryology, Istanbul University Cerrahpasa Faculty of Medicine, Istanbul§Department of Histology and Embryology, Kocaeli University Faculty of Medicine, Kocaeli||Department of Oral Implantology, Istanbul University Faculty of Dentistry, Istanbul, Turkey.
Gokhan
G
Guven
B Alper
BA
Gultekin
Gamze Senol
GS
Guven
Elif
E
Guzel
Selenay
S
Furat
Selim
S
Ersanli
D000287:Administration, Topical; D000818:Animals; D000077214:Becaplermin; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D016025:Bone Transplantation; D003094:Collagen; D006168:Guinea Pigs; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013977:Tibia / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014945:Wound Healing
D055396:Bone Morphogenetic Protein 2; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D000077214:Becaplermin; D003094:Collagen