Molecular evolution of epizootic hemorrhagic disease viruses in North America based on historical isolates using motif fingerprints.
Epizootic hemorrhagic disease virus (EHDV) is an orbivirus of the Reoviridae family that has significant impact on wild and captive white-tailed deer. Although closely related to bluetongue virus that can cause disease in sheep and cattle, North American EHDV historically has not been associated with disease in cattle or sheep. Severe disease in cattle has been reported with other EHDV strains from East Asia and the Middle East. To understand the potential role of viral genetics in the epidemiology of epizootic hemorrhagic disease, a molecular characterization of North American EHDV strains from 1955 to 2012 was conducted via conventional phylogenetic analysis and a new classification approach using motif fingerprint patterns. Overall, this study indicates that the genetic make-up of EHDV populations in North America have slowly evolved over time. The data also suggested limited reassortment events between serotypes 1 and 2 and introduces a new analysis tool for more detailed sequence pattern analysis.
Arthropod-Borne Animal Diseases Research Unit, Center for Grain and Animal Health Research, United States Department of Agriculture, Agricultural Research Service, 1515 College Ave., Manhattan, KS, 66502, USA. william.wilson@ars.usda.gov.
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Wilson
Arthropod-Borne Animal Diseases Research Unit, Center for Grain and Animal Health Research, United States Department of Agriculture, Agricultural Research Service, 1515 College Ave., Manhattan, KS, 66502, USA.|Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
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Ruder
Arthropod-Borne Animal Diseases Research Unit, Center for Grain and Animal Health Research, United States Department of Agriculture, Agricultural Research Service, 1515 College Ave., Manhattan, KS, 66502, USA.
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Jasperson
U.S. Meat Animal Research Center, United States Department of Agriculture, Agricultural Research Service, Clay Center, NE, USA.
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Smith
Assay Development and Virology, Lawrence Livermore National Laboratories, Livermore, CA, USA.|InSilixa Inc., Sunnyvale, CA, USA.
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Naraghi-Arani
Assay Development and Virology, Lawrence Livermore National Laboratories, Livermore, CA, USA.|Cerus Corporation, Concord, CA, USA.
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Lenhoff
Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
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Stallknecht
Orion Integrated Biosciences Inc., New Rochelle, NY, USA.
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Valdivia-Granda
Orion Integrated Biosciences Inc., New Rochelle, NY, USA.
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Sheoran
D000818:Animals; D002417:Cattle; D019143:Evolution, Molecular; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics*; D007313:Insecta / Q000821:virology; D009656:North America; D010802:Phylogeny; D012088:Reoviridae Infections / Q000662:veterinary / Q000821:virology
Epizootic hemorrhagic disease; Molecular evolution; Motif fingerprint