Treatment Paradigms for Retinal and Macular Diseases Using 3-D Retina Cultures Derived From Human Reporter Pluripotent Stem Cell Lines.
We discuss the use of pluripotent stem cell lines carrying fluorescent reporters driven by retinal promoters to derive three-dimensional (3-D) retina in culture and how this system can be exploited for elucidating human retinal biology, creating disease models in a dish, and designing targeted drug screens for retinal and macular degeneration. Furthermore, we realize that stem cell investigations are labor-intensive and require extensive resources. To expedite scientific discovery by sharing of resources and to avoid duplication of efforts, we propose the formation of a Retinal Stem Cell Consortium. In the field of vision, such collaborative approaches have been enormously successful in elucidating genetic susceptibility associated with age-related macular degeneration.
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States 2Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Rossukon
R
Kaewkhaw
National Therapeutics for Rare and Neglected Diseases, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, United States.
Manju
M
Swaroop
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
Kohei
K
Homma
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
Jutaro
J
Nakamura
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
Matthew
M
Brooks
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
Koray Dogan
KD
Kaya
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
Vijender
V
Chaitankar
National Therapeutics for Rare and Neglected Diseases, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, United States.
Sam
S
Michael
National Therapeutics for Rare and Neglected Diseases, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, United States.
Gregory
G
Tawa
iPSC Core, Center for Molecular Medicine, National Heart, Lung, and Blood Institute, Bethesda, Maryland, United States.
Jizhong
J
Zou
The New York Stem Cell Foundation Research Institute, New York, New York, United States.
Mahendra
M
Rao
National Therapeutics for Rare and Neglected Diseases, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, United States.
Wei
W
Zheng
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
Tiziana
T
Cogliati
Neurobiology-Neurodegeneration & Repair Laboratory National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
Anand
A
Swaroop
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D017930:Genes, Reporter / Q000502:physiology; D006801:Humans; D008268:Macular Degeneration / Q000378:metabolism / Q000628:therapy; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D012162:Retinal Degeneration / Q000378:metabolism / Q000628:therapy; D055351:Retinal Neurons / Q000166:cytology; D055213:Retinal Pigment Epithelium / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*