Regenerative and reparative effects of human chorion-derived stem cell conditioned medium on photo-aged epidermal cells.
Epidermal cells are an important regenerative source for skin wound healing. Aged epidermal cells have a low ability to renew themselves and repair skin injury. Ultraviolet (UV) radiation, particularly UVB, can cause photo-aging of the skin by suppressing the viability of human epidermal cells. A chorion-derived stem cell conditioned medium (CDSC-CNM) is thought to have regenerative properties. This study aimed to determine the regenerative effects of CDSC-CNM on UVB-induced photo-aged epidermal cells. Epidermal cells were passaged four times and irradiated with quantitative UVB, and non-irradiated cells served as a control group. Cells were then treated with different concentrations of CDSC-CNM. Compared to the non-irradiated group, the proliferation rates and migration rates of UVB-induced photo-aged epidermal cells significantly decreased (p < 0.05) with increasing intracellular radical oxygen species (ROS) generation and DNA damage. After treatment with CDSC-CNM, photo-aged epidermal cells significantly improved their viability, and their ROS generation and DNA damage decreased. The secretory factors in CDSC-CNM, including epidermal growth factor (EGF), transforming growth factor-β (TGF-β), interleukin (IL)-6, and IL-8 and the related signaling pathway protein levels, increased compared to the control medium (CM). The potential regenerative and reparative effects of CDSC-CNM indicate that it may be a candidate material for the treatment of prematurely aged skin. The functions of the secretory factors and the mechanisms of CDSC-CNM therapy deserve further attention.
a Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital , Beijing , China.|b Key Laboratory of Wound Repair and Regeneration of PLA, the First Affiliated Hospital, General Hospital of PLA , Beijing , China.
Qiankun
Q
Li
c Department of Pharmacy , General Hospital of Beijing Military Region , DongCheng District, Beijing , China.
Yan
Y
Chen
b Key Laboratory of Wound Repair and Regeneration of PLA, the First Affiliated Hospital, General Hospital of PLA , Beijing , China.
Kui
K
Ma
b Key Laboratory of Wound Repair and Regeneration of PLA, the First Affiliated Hospital, General Hospital of PLA , Beijing , China.
Along
A
Zhao
b Key Laboratory of Wound Repair and Regeneration of PLA, the First Affiliated Hospital, General Hospital of PLA , Beijing , China.
Cuiping
C
Zhang
a Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital , Beijing , China.|b Key Laboratory of Wound Repair and Regeneration of PLA, the First Affiliated Hospital, General Hospital of PLA , Beijing , China.
Xiaobing
X
Fu
D000975:Antioxidants / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002465:Cell Movement / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology*; D020552:Comet Assay; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004249:DNA Damage; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects / Q000528:radiation effects*; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects*; D013234:Stem Cells / Q000166:cytology*; D014466:Ultraviolet Rays
D000975:Antioxidants; D017077:Culture Media, Conditioned; D016207:Cytokines; D005456:Fluorescent Dyes; C579095:MCL1 protein, human; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D017382:Reactive Oxygen Species; D048049:Extracellular Signal-Regulated MAP Kinases
DNA damage; ROS; cell cycle; epidermal cell; photo-aging