Purification and characterization of biosurfactant produced by Bacillus licheniformis Y-1 and its application in remediation of petroleum contaminated soil.
In our previous research, a petroleum degrading bacteria strain Bacillus licheniformis Y-1 was obtained in Dagang Oilfield which had the capability of producing biosurfactant. This biosurfactant was isolated and purified in this work. The biosurfactant produced by strain Y-1 had the capability to decrease the surface tension of water from 74.66 to 27.26mN/m, with the critical micelle concentration (CMC) of 40mg/L. The biosurfactant performed not only excellent stabilities against pH, temperature and salinity, but also great emulsifying activities to different kinds of oil, especially the crude oil. According to the results of FT-IR spectrum and (1)H NMR spectrum detection, the surfactant was determined to be a cyclic lipopeptide. Furthermore, through the addition of surfactant, the effect of petroleum contaminated soil remediation by fungi got a significant improvement.
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China.
Boqun
B
Liu
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China. Electronic address: shevqunko@163.com.
Jinpeng
J
Liu
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China.
Meiting
M
Ju
Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, PR China.
Xiaojing
X
Li
College of Life Sciences, Nankai University, Tianjin 300071, PR China.
Qilin
Q
Yu
D000069976:Bacillus licheniformis / Q000737:chemistry*; D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry*
D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D013501:Surface-Active Agents
Bacillus licheniformis; Biosurfactant; Petroleum; Purification; Soil