MicroRNA-210 promotes sensory axon regeneration of adult mice in vivo and in vitro.
Axon regeneration as a critical step in nerve repairing and remodeling after peripheral nerve injury relies on regulation of gene expression. MicroRNAs are emerging to be important epigenetic regulators of gene expression to control axon regeneration. Here we used a novel in vivo electroporation approach to transfect microRNA-210 (miR-210) or siRNAs to adult mice dorsal root ganglion (DRG) neurons, measured the axon length 3days after sciatic nerve crush or dissociated DRG cultures in vitro to detect the effect of miR-210 in sensory axon regeneration. Importantly, we found that miR-210 overexpression could promote sensory axon regeneration and inhibit apoptsosis by ephrin-A3 (EFNA3). In addition, inhibition of endogenous miR-210 in DRG neurons impaired axon regeneration in vitro and in vivo, the regulatory effect of miR-210 was mediated by increased expression of EFNA3 because downregulation of EFNA3 fully rescued axon regeneration. We thus demonstrate that miR-210 is a new physiological regulator of sensory axon regeneration, and EFNA3 may be the functional target of miR-210. We conclude that miR-210 may play an important role in sensory axon regeneration.
Department of Orthopaedic Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110000, China; Guilin Medical University, Guilin, Guangxi, 541001, China.
Yi-Wen
YW
Hu
Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, China.
Jing-Jing
JJ
Jiang
Guilin Medical University, Guilin, Guangxi, 541001, China.
Yan-Gao
Guilin Medical University, Guilin, Guangxi, 541001, China.
Rui-Ying
RY
Wang
Department of Orthopaedic Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, 110000, China. Electronic address: tuguanjun188@yeah.net.
Guan-Jun
GJ
Tu
D000818:Animals; D017209:Apoptosis; D001369:Axons / Q000502:physiology*; D002478:Cells, Cultured; D018274:Electroporation; D036389:Ephrin-B3 / Q000235:genetics / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009416:Nerve Regeneration; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics; D012584:Sciatic Nerve / Q000293:injuries; D011984:Sensory Receptor Cells / Q000166:cytology / Q000502:physiology*
D036389:Ephrin-B3; C549325:MIRN210 microRNA, mouse; D035683:MicroRNAs; D034741:RNA, Small Interfering
Axon regeneration; DRG; Electroporation; MicroRNA-210; Peripheral nerve injury