TLR2/NFκB signalling regulates endogenous IL-6 release from marrow-derived mesenchymal stromal cells to suppress the apoptosis of PC12 cells injured by oxygen and glucose deprivation.
Two previous studies published by our group identified that mesenchymal stromal cells (MSCs) conferred neuroprotection in a rat model of hypoxic-ischaemic brain damage (HIBD), and that MSCs secreted abundant interleukin-6 (IL‑6) when co‑cultured with oxygen and glucose deprivation (OGD)‑injured PC12 cells. The present study has further investigated the role of IL‑6, and explored potential signalling pathways in vitro. In vitro models were established by co‑culturing OGD‑injured PC12 cells with MSCs. Subsequently, the expression levels of the signalling molecules, Toll‑like receptor 2 (TLR2)/nuclear factor κB (NFκB), and IL‑6 were altered separately in this in vitro model by treatment with an agonist, antagonist, siRNA or overexpression adenovirus. The expression levels of B cell lymphoma‑associated X (Bax), TLR2, NFκB and IL‑6 were detected by western blot analysis, real‑time polymerase chain reaction or ELISA. The resting membrane potential (RMP) of the PC12 cells was analysed by whole‑cell patch‑clamp recordings. Compared with controls or the PC12 co‑culture group, the MSC co‑cultured group induced less expression of Bax, but more IL‑6 secretion. Up- or down-regulation of the TLR2/NFκB signalling pathway resulted in a corresponding increase or decrease in the IL‑6 expression level in the MSCs. Co‑culture with siIL‑6‑MSCs increased the expression levels of Bax and increased the RMP in the OGD PC12 cells. In conclusion, the release of IL‑6 from MSCs was regulated via the TLR2/NFκB signalling pathway. Endogenous IL‑6 reduced apoptosis and protected OGD‑injured PC12 cells when they were co‑cultured with MSCs. The present study has reported a novel immunomodulatory effect of the microenvironment of neural damage during MSC cytotherapy.
Children's Nutrition Research Centre, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Xia
X
Shi
Children's Nutrition Research Centre, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Jingjing
J
Liu
Children's Nutrition Research Centre, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Ting
T
Yang
Children's Nutrition Research Centre, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Yun
Y
Zhang
Children's Nutrition Research Centre, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Tingyu
T
Li
Children's Nutrition Research Centre, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China.
Jie
J
Chen
D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D015687:Cell Hypoxia; D018920:Coculture Techniques; D005260:Female; D005947:Glucose / Q000172:deficiency*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
D015850:Interleukin-6; D016328:NF-kappa B; C495343:Tlr2 protein, rat; D051195:Toll-Like Receptor 2; D005947:Glucose