Self-Assembly of Multi-nanozymes to Mimic an Intracellular Antioxidant Defense System.
In this work, for the first time, we constructed a novel multi-nanozymes cooperative platform to mimic intracellular antioxidant enzyme-based defense system. V2 O5 nanowire served as a glutathione peroxidase (GPx) mimic while MnO2 nanoparticle was used to mimic superoxide dismutase (SOD) and catalase (CAT). Dopamine was used as a linker to achieve the assembling of the nanomaterials. The obtained V2 O5 @pDA@MnO2 nanocomposite could serve as one multi-nanozyme model to mimic intracellular antioxidant enzyme-based defense procedure in which, for example SOD, CAT, and GPx co-participate. In addition, through assembling with dopamine, the hybrid nanocomposites provided synergistic antioxidative effect. Importantly, both in vitro and in vivo experiments demonstrated that our biocompatible system exhibited excellent intracellular reactive oxygen species (ROS) removal ability to protect cell components against oxidative stress, showing its potential application in inflammation therapy.
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
Yanyan
Y
Huang
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.
Zhen
Z
Liu
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
Chaoqun
C
Liu
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
Enguo
E
Ju
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
Yan
Y
Zhang
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China. jren@ciac.ac.cn.
Jinsong
J
Ren
Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China. xqu@ciac.ac.cn.
Xiaogang
X
Qu
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D053770:Nanowires / Q000737:chemistry; D010031:Otitis / Q000139:chemically induced / Q000473:pathology; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017968:Vanadium Compounds / Q000737:chemistry
D000975:Antioxidants; D001672:Biocompatible Materials; D017382:Reactive Oxygen Species; D017968:Vanadium Compounds; C066075:vanadium pentoxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
antioxidant defense system; nanozymes; oxidative stress; reactive oxygen species; self-assembly