Kruppel-like factor 4 (KLF-4) inhibits the epithelial-to-mesenchymal transition and proliferation of human endometrial carcinoma cells.
Kruppel-like factors (KLFs) are a group of transcriptional regulators, being tumor-suppressive in various types of cancers, but not clear in human endometrial carcinoma (EC). We investigated the KLF-4 expression in both mRNA and protein levels in 29 EC specimens with RT-qPCR and Western blotting methods, and then to determine its promotion to Epithelial-to-mesenchymal transition (EMT) and proliferation of EC Ishikawa cells, via analyzing EMT-associated markers and via CCK-8 and colony forming assay. We found the downregulation of KLF-4 in the 29 EC specimens, correlating with the EC malignance. Moreover, we confirmed reduced levels of EMT and cell proliferation of Ishikawa cells post-KLF-4 overexpression. In conclusion, the significantly reduced KLF-4 correlated with the EC malignance. And the overexpressed KLF-4 promoted the EMT and proliferation of EC cells in vitro. The present study recognized the tumor suppressive role of KLF-4 in EC.
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Xiaoli
X
Wang
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Xiaoyan
X
Li
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Cuiping
C
Huang
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Lei
L
Li
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Hongmei
H
Qu
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Xiaoyan
X
Yu
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Huijie
H
Ni
a Department of Obstetrics and Gynecology , Yantai Yuhuangding Hospital of Qingdao University , Yantai , Shandong , China.
Qing
Q
Cui
D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*
D014408:Biomarkers, Tumor; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors
Endometrial carcinoma; Kruppel-like factor 4 (KLF-4); epithelial-to-mesenchymal transition (EMT); proliferation