Redox proteomic analysis of serum from aortic anerurysm patients: insights on oxidation of specific protein target.
oxidative stress is undoubtedly one of the main players in abdominal aortic aneurysm (AAA) pathophysiology. Recent studies in AAA patients reported an increase in the indices of oxidative damage at the tissue level and in biological fluids coupled with the loss of counter-regulatory mechanisms of protection from oxidative stress. We recently reported, in a proteomic analysis of AAA patient sera, changes in the expression of several proteins exerting important modulatory activities on cellular proliferation, differentiation and response to damage. This study aimed to explore the involvement of protein oxidation, at peripheral levels, in AAA.



a redox proteomic approach was used to investigate total and specific protein carbonylation and protein-bound 4-hydroxy-2-nonenal (HNE) in the serum of AAA patients compared with age-matched controls.



our results show increased oxidative damage to protein as indexed by the total carbonyl levels and total protein-bound HNE. By redox proteomics we identified specific carbonylation of three serum proteins: serum retinol-binding protein, vitamin D-binding protein and fibrinogen α-chain HNE. We also identified increased protein-bound HNE levels for hemopexin, IgK chain C region and IgK chain V-III region SIE. In addition we found a high correlation between specific protein carbonylation and protein-bound HNE and the aortic diameter. Moreover the analysis of serum proteins with antioxidant activity demonstrates the oxidation of albumin together with the overexpression of transferrin, haptoglobin and HSPs 90, 70, 60 and 32.



this study support the involvement of oxidative stress in the pathogenesis of AAA and might provide a further degree of knowledge in the cause-effect role of oxidative stress shedding new light on the molecular candidates involved in the disease.
Department of Cardiothoracic Surgery, West of Scotland Heart and Lung Centre, Golden Jubilee National Hospital, Agamemnon Street Clydebank, Glasgow G81 4DY, UK. Cristiano.spadaccio@gjnh.scot.nhs.uk and Department of Cardiovascular Sciences, University Campus Bio Medico of Rome, Italy.
Cristiano
C
Spadaccio
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
Raffaella
R
Coccia
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
Marzia
M
Perluigi
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
Gilda
G
Pupo
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
Maria Eugenia
ME
Schininà
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
Alessandra
A
Giorgi
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
Carla
C
Blarzino
Cardiac Surgery Centre Cardiologique du Nord de Saint-Denis, Paris, France.
Francesco
F
Nappi
Department of Cardiothoracic Surgery, West of Scotland Heart and Lung Centre, Golden Jubilee National Hospital, Agamemnon Street Clydebank, Glasgow G81 4DY, UK. Cristiano.spadaccio@gjnh.scot.nhs.uk.
Fraser W
FW
Sutherland
Department of Cardiovascular Sciences, University Campus Bio Medico of Rome, Italy.
Massimo
M
Chello
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
Fabio
F
Di Domenico
D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D001014:Aortic Aneurysm / Q000097:blood*; D015415:Biomarkers; D001798:Blood Proteins; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D013312:Stress, Physiological
D000975:Antioxidants; D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome