Terpenoids from Flueggea virosa and their anti-hepatitis C virus activity.
Phytochemical study of the methanolic root extract of Flueggea virosa allowed for the characterization of 18 non-alkaloid terpenoids. Their structures have skeletons composed of six rearranged ent-podocarpanes, 11 ent-podocarpanes, and a 3,4-seco-30-nor-friedelane. These were characterized based on 2D NMR, IR, UV, and MS spectroscopic analysis and their absolute configurations were determined by single-crystal X-ray studies, as well as by (1)H NMR spectroscopic analysis for the corresponding chiral derivatives. The isolates were evaluated for therapeutic potential against hepatitis C virus (HCV) infection to human hepatoma Huh7.5 cells.
School of Pharmacy, China Medical University, Taichung 40402, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan. Electronic address: chaochihhua@hotmail.com.
Chih-Hua
CH
Chao
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan.
Ju-Chien
JC
Cheng
Department of Pharmacy, National Cheng Kung University, Tainan 70101, Taiwan.
De-Yang
DY
Shen
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.
Hui-Chi
HC
Huang
School of Pharmacy, China Medical University, Taichung 40402, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan; Center for Molecular Medicine, China Medical University Hospital, Taichung 40447, Taiwan; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Yang-Chang
YC
Wu
Department of Pharmacy, National Cheng Kung University, Tainan 70101, Taiwan; Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 90741, Taiwan. Electronic address: tswu@mail.ncku.edu.tw.
Tian-Shung
TS
Wu
D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D019665:Euphorbiaceae / Q000737:chemistry*; D016174:Hepacivirus / Q000187:drug effects*; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*
D000998:Antiviral Agents; D014315:Triterpenes
3,4-seco-30-nor-Friedelane; Anti-hepatitis C virus; Euphorbiaceae; Flueggea virosa; ent-Podocarpane