Contact and Repellent Activities of the Essential Oil from Juniperus formosana against Two Stored Product Insects.
The chemical composition of the essential oil from Juniperus formosana leaves and its contact and repellent activities against Tribolium castaneum and Liposcelis bostrychophila adults were investigated. The essential oil of J. formosana leaves was obtained by hydrodistillation and analyzed by GC-MS. A total of 28 components were identified and the main compounds in the essential oil were α-pinene (21.66%), 4-terpineol (11.25%), limonene (11.00%) and β-phellandrene (6.63%). The constituents α-pinene, 4-terpineol and d-limonene were isolated from the essential oil. It was found that the essential oil exhibited contact activity against T. castaneum and L. bostrychophila adults (LD50 = 29.14 μg/adult and 81.50 µg/cm², respectively). The compound 4-terpineol exhibited the strongest contact activity (LD50 = 7.65 μg/adult). In addition, data showed that at 78.63 nL/cm², the essential oil and the three isolated compounds strongly repelled T. castaneum adults. The compounds α-pinene and d-limonene reached the same level (Class V) of repellency as DEET (p = 0.396 and 0.664) against L. bostrychophila at 63.17 nL/cm² after 2 h treatment. The results indicate that the essential oil and the isolated compounds have potential to be developed into natural insecticides and repellents to control insects in stored products.
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China. guoshanshan@mail.bnu.edu.cn.
Shanshan
S
Guo
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China. zwj0729@mail.bnu.edu.cn.
Wenjuan
W
Zhang
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China. liangjunyu@nwnu.edu.cn.
Junyu
J
Liang
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China. youchunxue@mail.bnu.edu.cn.
Chunxue
C
You
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China. gengzhufeng@bnu.edu.cn.|Analytical and Testing Center, Beijing Normal University, Beijing 100875, China. gengzhufeng@bnu.edu.cn.
Zhufeng
Z
Geng
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China. wangchengfang@mail.bnu.edu.cn.|China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Xicheng District, Beijing 100088, China. wangchengfang@mail.bnu.edu.cn.
Chengfang
C
Wang
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China. dushushan@bnu.edu.cn.
Shushan
S
Du
D000818:Animals; D000081008:Bicyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D061353:Food Storage; D006801:Humans; D007302:Insect Repellents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification; D018653:Juniperus / Q000737:chemistry*; D000077222:Limonene; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014231:Tribolium / Q000187:drug effects
D000081008:Bicyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D007302:Insect Repellents; D007306:Insecticides; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; C058582:beta-phellandrene; D000077222:Limonene; C005451:alpha-pinene
Juniperus formosana; Liposcelis bostrychophila; Tribolium castaneum; contact toxicity; repellency