[Effect of MiR-200b on retinal endothelial cell function in high-glucose condition and the mechanism].
OBJECTIVE
To investigate the effect of MiR-200b on human retinal endothelial cells (hRECs) cultured in high glucose and explore the mechanism.


METHODS
hRECs cultured in high glucose or in normal media were examined for MiR-200b mRNA expression using real-time PCR. The effect of MiR-200b transfection on hREC proliferation in high-glucose culture was evaluated with MTT assay, and real-time PCR and Western blotting were performed to determine vascular endothelial growth factor (VEGF) and transforming growth factor β1 (TGFβ1) expression in the transfected cells.


RESULTS
The cells in high-glucose culture showed significantly decreased MiR-200b expression and active proliferation. Compared with those in normal control cells, VEGF and TGFβ1 mRNA and protein expressions increased markedly in cells cultured in high glucose (P<0.05). MiR-200b transfection of the cells caused significantly increased cellular expression of MiR-200b but decreased expression levels of VEGF and TGFβ1 mRNA and protein, and suppressed hREC proliferation in high glucose culture (P<0.05).


CONCLUSIONS
MiR-200b can regulate REC growth and proliferation by changing VEGF and TGFβ1 expressions and thus play a role in the pathogenesis and progression of diabetic retinopathy.
Department of Ophthalmology, Second Xiangya Hospital of Central South University, Changsha 410011, China. E-mail: 1073427667@qq.com.
Qun
Q
Jiang
Xiao-Hua
XH
Zhu
Xin-Min
XM
Liu
Jian-Ming
JM
Liu
D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D003930:Diabetic Retinopathy; D042783:Endothelial Cells / Q000166:cytology*; D005947:Glucose / Q000737:chemistry; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D012160:Retina / Q000166:cytology*; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D003470:Culture Media; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D005947:Glucose