Three dimensional electron microscopy and in silico tools for macromolecular structure determination.
Recently, structural biology witnessed a major tool - electron microscopy - in solving the structures of macromolecules in addition to the conventional techniques, X-ray crystallography and nuclear magnetic resonance (NMR). Three dimensional transmission electron microscopy (3DTEM) is one of the most sophisticated techniques for structure determination of molecular machines. Known to give the 3-dimensional structures in its native form with literally no upper limit on size of the macromolecule, this tool does not need the crystallization of the protein. Combining the 3DTEM data with in silico tools, one can have better refined structure of a desired complex. In this review we are discussing about the recent advancements in three dimensional electron microscopy and tools associated with it.
Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India.
Subhomoi
S
Borkotoky
Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India.
Chetan Kumar
CK
Meena
Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India.
Mohammad Wahab
MW
Khan
Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry-605014, India.
Ayaluru
A
Murali
CTF correction; cryo-EM; segmentation; single particle analysis