Enhanced isopropanol and n-butanol production by supplying exogenous acetic acid via co-culturing two clostridium strains from cassava bagasse hydrolysate.
The focus of this study was to produce isopropanol and butanol (IB) from dilute sulfuric acid treated cassava bagasse hydrolysate (SACBH), and improve IB production by co-culturing Clostridium beijerinckii (C. beijerinckii) with Clostridium tyrobutyricum (C. tyrobutyricum) in an immobilized-cell fermentation system. Concentrated SACBH could be converted to solvents efficiently by immobilized pure culture of C. beijerinckii. Considerable solvent concentrations of 6.19 g/L isopropanol and 12.32 g/L butanol were obtained from batch fermentation, and the total solvent yield and volumetric productivity were 0.42 g/g and 0.30 g/L/h, respectively. Furthermore, the concentrations of isopropanol and butanol increased to 7.63 and 13.26 g/L, respectively, under the immobilized co-culture conditions when concentrated SACBH was used as the carbon source. The concentrations of isopropanol and butanol from the immobilized co-culture fermentation were, respectively, 42.62 and 25.45 % higher than the production resulting from pure culture fermentation. The total solvent yield and volumetric productivity increased to 0.51 g/g and 0.44 g/L/h when co-culture conditions were utilized. Our results indicated that SACBH could be used as an economically favorable carbon source or substrate for IB production using immobilized fermentation. Additionally, IB production could be significantly improved by co-culture immobilization, which provides extracellular acetic acid to C. beijerinckii from C. tyrobutyricum. This study provided a technically feasible and cost-efficient way for IB production using cassava bagasse, which may be suitable for industrial solvent production.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
Shaozhi
S
Zhang
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
Chunyun
C
Qu
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
Xiaoyan
X
Huang
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
Yukai
Y
Suo
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
Zhengping
Z
Liao
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China. jufwang@scut.edu.cn.
Jufang
J
Wang
D020001:1-Butanol / Q000378:metabolism*; D019840:2-Propanol / Q000378:metabolism*; D019342:Acetic Acid / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D018914:Cells, Immobilized / Q000382:microbiology; D002482:Cellulose / Q000737:chemistry*; D003013:Clostridium / Q000145:classification / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000378:metabolism; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D002365:Manihot / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry
D003470:Culture Media; D013464:Sulfuric Acids; D002244:Carbon; D020001:1-Butanol; D002482:Cellulose; C027433:bagasse; D019840:2-Propanol; C033158:sulfuric acid; D019342:Acetic Acid
Cassava bagasse hydrolysate; Fibrous-bed bioreactor; Immobilized-cell co-culture; Isopropanol-butanol fermentation; Repeated batch fermentation