Carbon dioxide capture using Escherichia coli expressing carbonic anhydrase in a foam bioreactor.
The present study reports CO2 capture and conversion to bicarbonate using Escherichia coli expressing carbonic anhydrase (CA) on its cell surface in a novel foam bioreactor. The very large gas-liquid interfacial area in the foam bioreactor promoted rapid CO2 absorption while the CO2 in the aqueous phase was subsequently converted to bicarbonate ions by the CA. CO2 gas removal in air was investigated at various conditions such as gas velocity, cell density and CO2 inlet concentration. Regimes for kinetic and mass transfer limitations were defined. Very high removal rates of CO2 were observed: 9570 g CO2 m(-3) bioreactor h(-1) and a CO2 removal efficiency of 93% at 4% inlet CO2 when the gas retention time was 24 s, and cell concentration was 4 gdw L(-1). These performances are superior to earlier reports of experimental bioreactors using CA for CO2 capture. Overall, this bioreactor system has significant potential as an alternative CO2 capture technology.
a Department of Molecular Bioscience and Bioengineering , University of Hawaii at Manoa , Honolulu , HI , USA.
Stuart K
SK
Watson
a Department of Molecular Bioscience and Bioengineering , University of Hawaii at Manoa , Honolulu , HI , USA.
Zhenlin
Z
Han
a Department of Molecular Bioscience and Bioengineering , University of Hawaii at Manoa , Honolulu , HI , USA.
Wei Wen
WW
Su
b Department of Civil and Environmental Engineering , Duke University , Durham , NC , USA.
Marc A
MA
Deshusses
a Department of Molecular Bioscience and Bioengineering , University of Hawaii at Manoa , Honolulu , HI , USA.
Eunsung
E
Kan
D001639:Bicarbonates / Q000378:metabolism; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism*; D057965:Carbon Sequestration; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*
D001639:Bicarbonates; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases
Carbon capture; carbonic anhydrase; foam bioreactor; surface-displaying bacteria; whole cell biocatalyst