Bioengineering Lungs for Transplantation.
Whole lung extracellular matrix scaffolds can be created by perfusion of cadaveric organs with decellularizing detergents, providing a platform for organ regeneration. Lung epithelial engineering must address both the proximal airway cells that function to metabolize toxins and aid mucociliary clearance and the distal pneumocytes that facilitate gas exchange. Engineered pulmonary vasculature must support in vivo blood perfusion with low resistance and intact barrier function and be antithrombotic. Repopulating the native lung matrix with sufficient cell numbers in appropriate anatomic locations is required to enable organ function.
Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA.
Sarah E
SE
Gilpin
Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA.
Jonathan M
JM
Charest
Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA.
Xi
X
Ren
Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA. Electronic address: hott@mgh.harvard.edu.
Harald C
HC
Ott
D057005:Bioengineering / Q000379:methods*; D006801:Humans; D016040:Lung Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
Bioartificial lung; Endothelium; Epithelium; Lung regeneration; Organ culture; Tissue engineering