Supramolecular Hydrogel from Nanoparticles and Cyclodextrins for Local and Sustained Nanoparticle Delivery.
Injectable and biodegradable supramolecular hydrogel mPECT NP/α-CD(gel) composed of high-concentration nanoparticle dispersion (≤20% W/V) and α-cyclodextrins (α-CD) are prepared by a two-level physical cross-linking using amphiphilic block polymer methoxy poly(ethylene glycol)-b-poly(ε-caprolactone-co-1,4,8-trioxa[4.6]spiro-9-undecanone) (mPECT) and α-CD. The gelation behavior depends on the concentration of nanoparticles and α-CD. The viscoelasticity and shear thinning of mPECT NP/α-CD(gel) are confirmed. In vitro hydrogel erosion is demonstrated to be mainly a concentration-dependent dissociation process with general release of discrete mPECT nanoparticles about 50 nm that can be easily taken up by cells. The in vitro release behavior can be modulated by changing the concentration of nanoparticles or α-CD. In vitro and in vivo cytotoxicity study demonstrates its biocompatibility and biosafety. Gel formation after subcutaneous injection is also confirmed and mPECT NP/α-CD(gel) shows about 2 weeks retention time. This work validates the potential application for this supramolecular hydrogel in local and sustained delivery of nanoparticles.
Department of Polymer Science and Technology and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
Shuxin
S
Xu
Department of Polymer Science and Technology and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
Li
L
Yin
Department of Polymer Science and Technology and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
Yuzhang
Y
Xiang
Department of Polymer Science and Technology and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
Hongzhang
H
Deng
Department of Polymer Science and Technology and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
Liandong
L
Deng
Tianjin Life Science Research Center and School of basic medical sciences, Tianjin Medical University, Tianjin, 300072, China.
Hongxia
H
Fan
Tianjin Life Science Research Center and School of basic medical sciences, Tianjin Medical University, Tianjin, 300072, China.
Hua
H
Tang
Department of Polymer Science and Technology and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
Jianhua
J
Zhang
Department of Polymer Science and Technology and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
Anjie
A
Dong
D000818:Animals; D002152:Calorimetry, Differential Scanning; D016923:Cell Death; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations / Q000494:pharmacology*; D004705:Endocytosis; D005026:Ethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry; D014961:X-Ray Diffraction; D047391:alpha-Cyclodextrins / Q000737:chemistry*
D003692:Delayed-Action Preparations; D005026:Ethylene Glycols; D011091:Polyesters; D047391:alpha-Cyclodextrins; C476300:poly(epsilon-caprolactone)-b-poly(ethylene glycol); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
hydrogel; local delivery; nanoparticles; α-cyclodextrins