Protein-SIP in environmental studies.
Metaproteomics coupled to stable isotope probing (SIP) was established to detect metabolically active key players in microbial communities. Here, we discuss the current state of protein-based stable isotope probing (protein-SIP) and the perspectives of using different stable isotope atoms (i.e.13C,15N,18O,34/36S), multiple isotope labelling, the utilisation of substrates of major abundance and micro-pollutants [pesticides, herbicides and pharmaceuticals present in the environment at very low concentrations (ngμg/L)], and applications in complex model systems and in situ studies in the environment.
Helmholtz-Centre for Environmental Research - UFZ, Department of Molecular Systems Biology, Permoserstr. 15, 04318 Leipzig, Germany.
Nico
N
Jehmlich
Helmholtz-Centre for Environmental Research - UFZ, Department of Isotope Biogeochemistry, Permoserstr. 15, 04318 Leipzig, Germany.
Carsten
C
Vogt
Helmholtz-Centre for Environmental Research - UFZ, Department of Molecular Systems Biology, Permoserstr. 15, 04318 Leipzig, Germany; Helmholtz-Centre for Environmental Research - UFZ, Department of Environmental Biotechnology, Permoserstr. 15, 04318 Leipzig, Germany.
Vanessa
V
Lünsmann
Helmholtz-Centre for Environmental Research - UFZ, Department of Isotope Biogeochemistry, Permoserstr. 15, 04318 Leipzig, Germany.
Hans Hermann
HH
Richnow
Helmholtz-Centre for Environmental Research - UFZ, Department of Molecular Systems Biology, Permoserstr. 15, 04318 Leipzig, Germany; University of Leipzig, Faculty of Biosciences, Pharmacy and Psychology, Institute of Biochemistry, Brüderstr. 34, 04103 Leipzig, Germany; Aalborg University, Department of Chemistry and Bioscience, 9220 Aalborg, Denmark. Electronic address: martin.vonbergen@ufz.de.
Martin
M
von Bergen
D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D004785:Environmental Pollutants / Q000032:analysis*; D007553:Isotope Labeling / Q000379:methods*; D007554:Isotopes / Q000032:analysis*
D001426:Bacterial Proteins; D004785:Environmental Pollutants; D007554:Isotopes