Extensively drug-resistant bacteria are an independent predictive factor of mortality in 130 patients with spontaneous bacterial peritonitis or spontaneous bacteremia.
OBJECTIVE
To evaluate the epidemiology and outcomes of culture-positive spontaneous bacterial peritonitis (SBP) and spontaneous bacteremia (SB) in decompensated cirrhosis.


METHODS
We prospectively collected clinical, laboratory characteristics, type of administered antibiotic, susceptibility and resistance of bacteria to antibiotics in one hundred thirty cases (68.5% males) with positive ascitic fluid and/or blood cultures during the period from January 1, 2012 to May 30, 2014. All patients with SBP had polymorphonuclear cell count in ascitic fluid > 250/mm(3). In patients with SB a thorough study did not reveal any other cause of bacteremia. The patients were followed-up for a 30-d period following diagnosis of the infection. The final outcome of the patients was recorded in the end of follow-up and comparison among 3 groups of patients according to the pattern of drug resistance was performed.


RESULTS
Gram-positive-cocci (GPC) were found in half of the cases. The most prevalent organisms in a descending order were Escherichia coli (33), Enterococcus spp (30), Streptococcus spp (25), Klebsiella pneumonia (16), S. aureus (8), Pseudomanas aeruginosa (5), other Gram-negative-bacteria (GNB) (11) and anaerobes (2). Overall, 20.8% of isolates were multidrug-resistant (MDR) and 10% extensively drug-resistant (XDR). Health-care-associated (HCA) and/or nosocomial infections were present in 100% of MDR/XDR and in 65.5% of non-DR cases. Meropenem was the empirically prescribed antibiotic in HCA/nosocomial infections showing a drug-resistance rate of 30.7% while third generation cephalosporins of 43.8%. Meropenem was ineffective on both XDR bacteria and Enterococcus faecium (E. faecium). All but one XDR were susceptible to colistin while all GPC (including E. faecium) and the 86% of GNB to tigecycline. Overall 30-d mortality was 37.7% (69.2% for XDR and 34.2% for the rest of the patients) (log rank, P = 0.015). In multivariate analysis, factors adversely affecting outcome included XDR infection (HR = 2.263, 95%CI: 1.005-5.095, P = 0.049), creatinine (HR = 1.125, 95%CI: 1.024-1.236, P = 0.015) and INR (HR =1.553, 95%CI: 1.106-2.180, P = 0.011).


CONCLUSIONS
XDR bacteria are an independent life-threatening factor in SBP/SB. Strategies aiming at restricting antibiotic overuse and rapid identification of the responsible bacteria could help improve survival.
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Alexandra
A
Alexopoulou
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Larisa
L
Vasilieva
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Danai
D
Agiasotelli
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Kyriaki
K
Siranidi
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Sophia
S
Pouriki
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Athanasia
A
Tsiriga
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Marina
M
Toutouza
Alexandra Alexopoulou, Larisa Vasilieva, Danai Agiasotelli, Kyriaki Siranidi, Sophia Pouriki, Spyridon P Dourakis, 2 Department of Internal Μedicine, Hippokration Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Spyridon P
SP
Dourakis
D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D016470:Bacteremia / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology* / Q000401:mortality*; D003428:Cross Infection / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology* / Q000401:mortality*; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008103:Liver Cirrhosis / Q000150:complications* / Q000175:diagnosis / Q000401:mortality; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010538:Peritonitis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology* / Q000401:mortality*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents
Extensively drug-resistant bacteria; Multidrug-resistant bacteria; Spontaneous bacteremia; Spontaneous bacterial peritonitis; Susceptibility to antibiotics