Expression and Localization of Human Defensins in Palatine Tonsils.
Defensins are small antimicrobial peptides and effector components of innate immune responses. Recent studies have shed light on their beneficial functions for the prevention of infection and potential for development of new drugs. Here, we showed the expression profiles of human defensins in palatine tonsils with 3 different diseases: tonsillar hypertrophy, recurrent tonsillitis and focal infection of the tonsil. RT-PCR analysis and immunofluorescence revealed that the expression of human α-defensin 4 and β-defensin 3 (β3) in palatine tonsils with tonsillar hypertrophy was lower than that in recurrent tonsillitis and focal infection of the tonsil, suggesting that chronic inflammation induces defensin expression. Interestingly, β2 and β3 mRNAs were specifically expressed by palatine tonsil tissues but not in human peripheral blood mononuclear cells and mucosa of the small intestine. Additionally, we observed that exposure to a Toll-like receptor 4 ligand, lipopolysaccharide, which is used as a bacterial infection model, increases the production of β2 in culture supernatants from tonsillar epithelial cells in a dose-dependent manner. Taken together, these results indicate that β2 produced by tonsillar epithelial cells plays an important role in the innate immune response for bacterial infections.
Ryuta
R
Kamekura
Ryokichi
R
Imai
Kenichi
K
Takano
Keiji
K
Yamashita
Sumito
S
Jitsukawa
Tomonori
T
Nagaya
Fumie
F
Ito
Motoyasu
M
Hirao
Hiroshi
H
Tsubota
Tetsuo
T
Himi
D000328:Adult; D002478:Cells, Cultured; D023082:Defensins / Q000096:biosynthesis / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D008297:Male; D008856:Microscopy, Fluorescence; D014066:Palatine Tonsil / Q000000981:diagnostic imaging / Q000276:immunology / Q000378:metabolism*; D012313:RNA / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D014069:Tonsillitis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
D023082:Defensins; D012313:RNA