Preparation of Biocatalytic Microparticles by Interfacial Self-Assembly of Enzyme-Nanoparticle Conjugates Around a Cross-Linkable Core.
Rational design of hierarchical interfacial assembly of reusable biocatalytic microparticles is described in this chapter. Specifically, purified enzymes and functionalized nanoparticles are electrostatically assembled at the interface of cross-linked microparticles which are formed through ring opening metathesis polymerization. The diameters of microparticle assemblies average 10μm, and they show enhanced kinetic efficiency as well as improved stability against heat, pH, and solvent denaturation when compared to stabilities of the corresponding native enzymes.
Department of Food Science, University of Massachusetts-Amherst, Amherst, Massachusetts, USA.
S M
SM
Andler
Department of Chemistry, University of Massachusetts-Amherst, Amherst, Massachusetts, USA.
L-S
LS
Wang
Department of Food Science, University of Massachusetts-Amherst, Amherst, Massachusetts, USA.
J M
JM
Goddard
Department of Chemistry, University of Massachusetts-Amherst, Amherst, Massachusetts, USA. Electronic address: rotello@chem.umass.edu.
V M
VM
Rotello
D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry*; D058105:Polymerization; D011489:Protein Denaturation; D012997:Solvents / Q000737:chemistry
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D012997:Solvents; D008049:Lipase; C431349:lipase B, Candida antarctica
Enzyme; Hierarchical assembly; Immobilization; Nano; Reusable