Nucleotide sequence analysis of the hypervariable region III of mitochondrial DNA in Thais.
This study analyzed the nucleotide sequences of the hypervariable region III (HVRIII) of mitochondrial DNA in Thai individuals. Buccal swab samples were randomly obtained from 100 healthy, unrelated, adult (18-60 years old), volunteer donors living in Thailand. Eighteen different haplotypes were found, of which 11 haplotypes were unique. The most frequent haplotypes observed were 522D-523D. Nucleotide transition from Thymine (T) to Cytosine (C) at position 489 (43%) was the most frequent substitution. Nucleotide transversions were also observed at position 433 (Adenine (A) to C, 1%) and position 499 (Guanine (G) to C, 1%). Fifty-three samples presented nucleotide insertion and deletion of C and A (CA) at position 514-523. Insertion of 1AC (3%) and 2AC (2%) were observed. Deletion of 1CA (53%) and 2CA (2%) at position 514-523 were revealed. The deletion of T at position 459 was observed. The haplotype diversity, random match probability, and discrimination power were calculated to be 0.7770, 0.2308, and 0.7692, respectively.
Division of Forensic Science, The Graduate School, Chiang Mai University, Chiang Mai, Thailand.
Punlop
P
Thongngam
Division of Biochemistry and Biochemical Technology, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
Worraanong
W
Leewattanapasuk
Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Tanin
T
Bhoopat
Division of Biochemistry and Biochemical Technology, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. Electronic address: padchanee.sangthong@cmu.ac.th.
Padchanee
P
Sangthong
D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D054643:INDEL Mutation; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013785:Thailand; D055815:Young Adult
D004272:DNA, Mitochondrial
Hypervariable region III (HVRIII); Mitochondrial DNA; Nucleotide sequence analysis; Polymerase chain reaction; Thai individuals