Toxicity of combined chromium(VI) and phenanthrene pollution on the seed germination, stem lengths, and fresh weights of higher plants.
Studies of the interaction and toxicity of pollutant combinations such as heavy metals and PAHs are of practical importance in the remediation and monitoring of the industrial soil environment. This study investigated the single and combined toxicity of chromium(VI) and phenanthrene on three important higher plants: mung beans (Phaseolus aureus), pakchoi cabbage (Brassica chinensis), and rice (Oryza sativa). In experiments using artificial soil matrix, the EC10 and EC20 of the two pollutants, alone and in combination, were analyzed with respect to seed germination, stem length, and above-ground fresh weight of these higher plants. The additive index method was used to evaluate the combined biological toxicity of chromium(VI) and phenanthrene. The results showed that the EC20 of chromium(VI) on the stem lengths of mung beans, pakchoi cabbage, and rice was 289, 248, and 550 mg kg(-1), respectively. The corresponding EC20 values for the fresh weights of the three plants were 334, 307, and 551 mg kg(-1). The EC20 of phenanthrene on the stem lengths of mung beans, pakchoi cabbage, and rice was 528, 426, and 628 mg kg(-1), respectively. The corresponding EC20 values for the fresh weights of the three plants were 696, 585, and 768 mg kg(-1). The EC20 of a combination of chromium(VI) and phenanthrene on the stem lengths of mung beans, pakchoi cabbage, and rice was 192, 173, and 279 mg kg(-1), respectively, and 200, 205, and 271 mg kg(-1) for the fresh weights of the three plants. The single and combined exposure of soil to chromium(VI) and phenanthrene had deleterious effects on plants in the early stage of growth. Overall, pakchoi cabbage was more sensitive than mung beans and rice. The two pollutants exerted synergistic effects on the stem lengths and above-ground fresh weights of both mung beans and rice but antagonistic effects on pakchoi cabbage. The results of this study also suggested pakchoi cabbage as a sensitive indicator of soil pollution.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.|Shanghai Academy of Environmental Sciences, Shanghai, 200233, China.
Shuangqing
S
Hu
Shanghai Academy of Environmental Sciences, Shanghai, 200233, China.
Hairong
H
Gu
College of Environmental Science and Engineering, Donghua University, Shanghai, 201620, China.
Chunyan
C
Cui
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China. ji@nju.edu.cn.
Rong
R
Ji
D001937:Brassica / Q000187:drug effects* / Q000254:growth & development; D002857:Chromium / Q000378:metabolism*; D018525:Germination / Q000187:drug effects; D012275:Oryza / Q000187:drug effects* / Q000254:growth & development; D027805:Phaseolus / Q000187:drug effects* / Q000254:growth & development; D010616:Phenanthrenes / Q000378:metabolism*; D018547:Plant Stems / Q000187:drug effects; D012989:Soil Pollutants / Q000378:metabolism*
D010616:Phenanthrenes; D012989:Soil Pollutants; D002857:Chromium; C074702:chromium hexavalent ion; C031181:phenanthrene
Chromium(VI); Combined effect; Fresh weight; Phenanthrene; Stem length