Development of a web-based tool for automated processing and cataloging of a unique combinatorial drug screen.
Facing totally resistant bacteria, traditional drug discovery efforts have proven to be of limited use in replenishing our depleted arsenal of therapeutic antibiotics. Recently, the natural anti-bacterial properties of metal ions in synergy with metal-coordinating ligands have shown potential for generating new molecule candidates with potential therapeutic downstream applications. We recently developed a novel combinatorial screening approach to identify compounds with copper-dependent anti-bacterial properties. Through a parallel screening technique, the assay distinguishes between copper-dependent and independent activities against Mycobacterium tuberculosis with hits being defined as compounds with copper-dependent activities. These activities must then be linked to a compound master list to process and analyze the data and to identify the hit molecules, a labor intensive and mistake-prone analysis. Here, we describe a software program built to automate this analysis in order to streamline our workflow significantly. We conducted a small, 1440 compound screen against M. tuberculosis and used it as an example framework to build and optimize the software. Though specifically adapted to our own needs, it can be readily expanded for any small- to medium-throughput screening effort, parallel or conventional. Further, by virtue of the underlying Linux server, it can be easily adapted for chemoinformatic analysis of screens through packages such as OpenBabel. Overall, this setup represents an easy-to-use solution for streamlining processing and analysis of biological screening data, as well as offering a scaffold for ready functionality expansion.
Department of Medicine, Division of Infectious Diseases, University of Alabama at Birmingham, 845 19th St S, Birmingham, AL 35294, United States. Electronic address: dalecki@uab.edu.
Alex G
AG
Dalecki
Department of Medicine, Division of Infectious Diseases, University of Alabama at Birmingham, 845 19th St S, Birmingham, AL 35294, United States. Electronic address: fwolsche@uab.edu.
Frank
F
Wolschendorf
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D057205:Automation, Laboratory; D002380:Catalogs, Drug as Topic; D003300:Copper / Q000494:pharmacology; D055808:Drug Discovery / Q000379:methods; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D020407:Internet; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D012984:Software
D000900:Anti-Bacterial Agents; D003300:Copper
Chemoinformatics; Combinatorial screen; Copper; High throughput screen; Tuberculosis