Biomarker-specific conjugated nanopolyplexes for the active coloring of stem-like cancer cells.
Stem-like cancer cells possess intrinsic features and their CD44 regulate redox balance in cancer cells to survive under stress conditions. Thus, we have fabricated biomarker-specific conjugated polyplexes using CD44-targetable hyaluronic acid and redox-sensible polyaniline based on a nanoemulsion method. For the most sensitive recognition of the cellular redox at a single nanoparticle scale, a nano-scattering spectrum imaging analyzer system was introduced. The conjugated polyplexes showed a specific targeting ability toward CD44-expressing cancer cells as well as a dramatic change in its color, which depended on the redox potential in the light-scattered images. Therefore, these polyaniline-based conjugated polyplexes as well as analytical processes that include light-scattering imaging and measurements of scattering spectra, clearly establish a systematic method for the detection and monitoring of cancer microenvironments.
Department of Radiology, College of Medicine, Yonsei University, Seoul 03722, Korea.
Yoochan
Y
Hong
Eugene
E
Lee
Jihye
J
Choi
Seungjoo
S
Haam
Jin-Suck
JS
Suh
Jaemoon
J
Yang
D014408:Biomarkers, Tumor / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid; D053758:Nanoparticles; D014411:Neoplastic Stem Cells; D010084:Oxidation-Reduction
D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid