Quantitative Microplate Assay for Real-Time Nuclease Kinetics.
Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.
Department of Neurochemistry, Stockholm University, Stockholm, Sweden.
Jonas
0000-0001-8813-1096
J
Eriksson
Department of Neurochemistry, Stockholm University, Stockholm, Sweden.
Ülo
Ü
Langel
D002384:Catalysis; D004720:Endonucleases / Q000737:chemistry*; D007700:Kinetics
D004720:Endonucleases