Increased numbers of CD23(+) CD21(hi) Bin-like B cells in human reactive and rheumatoid arthritis lymph nodes.
A unique population of CD23(+) CD21(high) B cells in inflamed nodes (Bin) has been shown to accumulate in lymph nodes (LNs) draining inflamed joints of TNF-transgenic (TNF-tg) mice. Bin cells contribute to arthritis flare in mice by distorting node architecture and hampering lymphatic flow, but their existence in human inflamed LNs has not yet been described. Here, we report the characterization of resident B-cell populations in fresh popliteal lymph nodes (PLNs) from patients with severe lower limb diseases (non-RA) and rheumatoid arthritis (RA) patients, and from banked, cryopreserved reactive and normal human LN single cell suspension samples. Bin-like B cells were shown to be significantly increased in reactive LNs, and strikingly elevated (>30% of total) in RA samples. Histopathology and immunofluorescence analyses were consistent with B follicular hyperplasia and histological alterations in RA vs. non-RA PLNs. This is the first description of Bin-like B cells in human inflamed LNs. Consistent with published mouse data, this population appears to be associated with inflammatory arthritis and distortion of LN architecture. Further analyses are necessary to assess the role of CD23(+) CD21(hi) Bin-like B cells in RA pathogenesis and arthritic flare.
Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA.
Igor I
II
Kuzin
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.|Department of Orthopaedics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Stephen L
SL
Kates
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.|Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Yawen
Y
Ju
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.|Department of Orthopaedics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Longze
L
Zhang
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.|Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Homaira
H
Rahimi
Center for Pediatric Biomed Research and Flow Cytometry Shared Resource Laboratory, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Wojciech
W
Wojciechowski
J.P. Wilmot Cancer Center, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Steven H
SH
Bernstein
Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.|J.P. Wilmot Cancer Center, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Richard
R
Burack
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.|Department of Orthopaedics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.
Edward M
EM
Schwarz
Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA.
Andrea
A
Bottaro
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D015415:Biomarkers; D006801:Humans; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D018655:Lymphocyte Count; D051379:Mice; D008822:Mice, Transgenic; D017464:Receptors, Complement 3d / Q000378:metabolism*; D017455:Receptors, IgE / Q000378:metabolism*
D015415:Biomarkers; D017464:Receptors, Complement 3d; D017455:Receptors, IgE
B cells; Inflammation; Lymph nodes; Rheumatoid arthritis; TNF