Purification and Immunoprotection Evaluation of AaHIV from Complex Venom Metalloproteinases of Deinagkistrodon acutus.
The aim of this study was to investigate the immunoprotective effects of AaHIV in mice. After purification, a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed. Bicinchoninic acid was used to determine the molecular weight and concentration of AaHIV. AaHIV, venom complex (VC), and phosphate buffered saline (PBS) were subsequently used to immunize the mice three times, and the blood was sampled 1 week after the third immunization to determine the serum immunoglobulin G (IgG) antibody titer. A skin-bleeding inhibition assay and toxin-eliminating assay were performed on the immunized mice. The purity and concentration of AaHIV were 86.6% and 1.20 mg/mL, respectively. The AaHIV group exhibited higher antibody titers than the VC group. The survival rate of the AaHIV group (7/10) was significantly higher than that of the PBS group (0/10) (P = 0.0031). The high titer of antibodies induced by AaHIV partially neutralized the bleeding activity of the Deinagkistrodon acutus venom complex.
Department of Emergent Rescue, Affiliated Southwest Hospital of the Third Military Medical University, Chongqing, 400038, People's Republic of China.
Lei
L
Zhang
Department of Emergent Rescue, Affiliated Southwest Hospital of the Third Military Medical University, Chongqing, 400038, People's Republic of China.
Cong
C
Chen
Department of Emergent Rescue, Affiliated Southwest Hospital of the Third Military Medical University, Chongqing, 400038, People's Republic of China.
Yuliang
Y
Cao
Department of Emergent Rescue, Affiliated Southwest Hospital of the Third Military Medical University, Chongqing, 400038, People's Republic of China.
Baifa
B
Xie
Department of Emergent Rescue, Affiliated Southwest Hospital of the Third Military Medical University, Chongqing, 400038, People's Republic of China.
Xiangyu
X
Chen
Department of Emergent Rescue, Affiliated Southwest Hospital of the Third Military Medical University, Chongqing, 400038, People's Republic of China.
Fanjie
F
Zeng
Department of Emergent Rescue, Affiliated Southwest Hospital of the Third Military Medical University, Chongqing, 400038, People's Republic of China. minghualiudoc@126.com.
Minghua
M
Liu
D000818:Animals; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000997:Antivenins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D001681:Biological Assay; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006470:Hemorrhage / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D045726:Metalloproteases / Q000037:antagonists & inhibitors* / Q000276:immunology; D051379:Mice; D012911:Snakes / Q000502:physiology; D016019:Survival Analysis
D000936:Antigen-Antibody Complex; D000997:Antivenins; D003435:Crotalid Venoms; D007074:Immunoglobulin G; D045726:Metalloproteases
AaHIV; Antibody; Deinagkistrodon acutus; Immunoprotection; Purification Method; SVMPs; Snake Venom Metalloproteinases