[Recent advances in preparative electromigration techniques for proteins].
Preparative electromigration techniques are a category of separation and preparation techniques based on differential electromigration principle. These techniques are widely applied in biomacromolecule and proteome researches. Related techniques mainly include preparative electrophoresis, preparative electrochromatography, preparative isoelectric focusing and free flow electrophoresis and so on. We review each kind of the preparative electromigration techniques on its apparatus designs, applications, advantages and disadvantages in detail. Microscale preparative electrophoresis is a hot spot in recent years. Its high resolution, high recovery and high efficiency make it a more prominent role in the biological sample analysis. In this review, we focus on some advances in microscale preparative electrophoresis and also give an outlook on preparative electromigration techniques in the future.
Feiran
F
Hao
Bin
B
Fu
Yangjun
Y
Zhang
Xiaohong
X
Qian
D004586:Electrophoresis; D007525:Isoelectric Focusing; D011506:Proteins / Q000737:chemistry*; D020543:Proteome
D011506:Proteins; D020543:Proteome