Proteomic and phosphoproteomic analysis reveals the response and defense mechanism in leaves of diploid wheat T. monococcum under salt stress and recovery.
Salinity is a major abiotic stress factor affecting crops production and productivity. Triticum monococcum is closely related to Triticum urartu (A(U)A(U)), which is used as a model plant of wheat A genome study. Here, salt stress induced dynamic proteome and phosphoproteome profiling was focused. The T. monococcum seedlings were initially treated with different concentrations of NaCl ranging from 80 to 320mM for 48h followed by a recovery process for 48h prior to proteomic and phosphoproteomic analysis. As a result, a total of 81 spots corresponding to salt stress and recovery were identified by MALDI-TOF/TOF-MS from 2-DE gels. These proteins were mainly involved in regulatory, stress defense, protein folding/assembly/degradation, photosynthesis, carbohydrate metabolism, energy production and transportation, protein metabolism, and cell structure. Pro-Q Diamond staining was used to detect the phosphoproteins. Finally, 20 spots with different phosphorylation levels during salt treatment or recovery compared with controls were identified. A set of potential salt stress response and defense biomarkers was identified, such as cp31BHv, betaine-aldehyde dehydrogenase, leucine aminopeptidase 2, Cu/Zn superoxide dismutase, and 2-Cys peroxiredoxin BAS1, which could lead to a better understanding of the molecular basis of salt response and defense in food crops.



Soil salinity reduces the yield of the major crops, which is one of the severest problems in irrigated agriculture worldwide. However, how crops response and defense during different levels of salt treatment and recovery processes is still unclear, especially at the post-translational modification level. T. monococcum is a useful model for common wheat. Thus, proteomic and phosphoproteomic analyses of T. monococcum leaves were performed in our study, which provided novel insights into the underlying salt response and defense mechanisms in wheat and other crops.
College of Life Science, Capital Normal University, 100048 Beijing, China; VCU Philips Institute for Oral Health Research, Virginia Commonwealth University, School of Dentistry, Department of Oral and Craniofacial Molecular Biology, 23298 Richmond, VA, USA.
Dong-Wen
DW
Lv
College of Life Science, Capital Normal University, 100048 Beijing, China.
Geng-Rui
GR
Zhu
College of Life Science, Capital Normal University, 100048 Beijing, China.
Dong
D
Zhu
College of Life Science, Capital Normal University, 100048 Beijing, China.
Yan-Wei
YW
Bian
College of Life Science, Capital Normal University, 100048 Beijing, China.
Xiao-Na
XN
Liang
College of Life Science, Capital Normal University, 100048 Beijing, China.
Zhi-Wei
ZW
Cheng
College of Life Science, Capital Normal University, 100048 Beijing, China.
Xiong
X
Deng
College of Life Science, Capital Normal University, 100048 Beijing, China. Electronic address: yanym@cnu.edu.cn.
Yue-Ming
YM
Yan
D010750:Phosphoproteins / Q000032:analysis*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification / Q000502:physiology; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D054712:Salinity; D055049:Salt Tolerance; D013312:Stress, Physiological; D014908:Triticum / Q000737:chemistry*
D010750:Phosphoproteins; D010940:Plant Proteins
Phosphoproteome; Proteome; Recovery; Salt stress; T. monococcum