[Expressions of Slug, ZEB1 and KISS-1 in gastric adenocarcinoma and their clinical significance].
OBJECTIVE
To identify potential markers for predicting invasion, metastasis, and prognosis of gastric adenocarcinoma (GAC).


METHODS
The expressions of Slug, ZEB1 and KISS-1 were detected immunohistochemically in 261 GAC tissues and 80 normal gastric tissues.


RESULTS
The positivity rates of Slug, ZEB1, and KISS-1 in gastric tissues were 2.5%, 1.3%, and 87.5%, respectively, significantly different from the rates of 62.1%, 28.4%, and 41.1% in GAC tissues (P<0.05). The expression level of Slug was significantly correlated with the depth of invasion, lymph node metastasis, and pTNM stages; the positivity rates of both ZEB1 and KISS-1 were significantly correlated with the tumor grade, depth of invasion, lymph node metastasis and pTNM stages. Slug expression was positively correlated with ZEB1 expression, and KISS-1 expression was inversely correlated with Slug and ZEB1 expressions. Kaplan-Meier analysis showed that the overall survival time of patients with positive expressions of Slug and ZEB1 was significantly shorter than that of the negative patients, and the survival time of patients positive for KISS-1 was significantly longer than the negative patients. COX multivariate analysis showed that positive Slug, ZEB1 and KISS-1 protein expressions and pTNM stages were independent prognostic factors of GAC (P<0.05).


CONCLUSIONS
The abnormal expressions of Slug, ZEB1 and KISS-1 may contribute to the tumorigenesis of GAC and are related with lymph node metastasis, pTNM stages, and prognosis of GAC. The combined detection of Slug, ZEB1, and KISS-1 expression has an important value in predicting the progression and prognosis of GAC.
Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233003, China. E-mail: zhou_lei03@163.com.
Lei
L
Zhou
Yong-Lian
YL
Hu
Shi-Wu
SW
Wu
Lan
L
Yu
Ze-Nong
ZN
Cheng
Bo
B
Zhu
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D059648:Kisspeptins / Q000378:metabolism*; D008207:Lymphatic Metastasis; D060787:Neoplasm Grading; D011379:Prognosis; D016016:Proportional Hazards Models; D000071250:Snail Family Transcription Factors; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism*; D000071799:Zinc Finger E-box-Binding Homeobox 1
D018398:Homeodomain Proteins; C106628:KISS1 protein, human; D059648:Kisspeptins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1