Predictive Blood Chemistry Parameters for Pansteatitis-Affected Mozambique Tilapia (Oreochromis mossambicus).
One of the largest river systems in South Africa, the Olifants River, has experienced significant changes in water quality due to anthropogenic activities. Since 2005, there have been various "outbreaks" of the inflammatory disease pansteatitis in several vertebrate species. Large-scale pansteatitis-related mortality events have decimated the crocodile population at Lake Loskop and decreased the population at Kruger National Park. Most pansteatitis-related diagnoses within the region are conducted post-mortem by either gross pathology or histology. The application of a non-lethal approach to assess the prevalence and pervasiveness of pansteatitis in the Olifants River region would be of great importance for the development of a management plan for this disease. In this study, several plasma-based biomarkers accurately classified pansteatitis in Mozambique tilapia (Oreochromis mossambicus) collected from Lake Loskop using a commercially available benchtop blood chemistry analyzer combined with data interpretation via artificial neural network analysis. According to the model, four blood chemistry parameters (calcium, sodium, total protein and albumin), in combination with total length, diagnose pansteatitis to a predictive accuracy of 92 percent. In addition, several morphometric traits (total length, age, weight) were also associated with pansteatitis. On-going research will focus on further evaluating the use of blood chemistry to classify pansteatitis across different species, trophic levels, and within different sites along the Olifants River.
National Institute of Standards and Technology (NIST), Material Measurement Laboratory, Chemical Sciences Division, Environmental Chemical Sciences Group, Hollings Marine Laboratory, Charleston, South Carolina, United States of America.
John A
JA
Bowden
Departments of Obstetrics and Gynecology, Medical University of South Carolina (MUSC), Charleston, South Carolina, United States of America.
Theresa M
TM
Cantu
Marine Resources Research Institute, South Carolina Department of Natural Resources, Hollings Marine Laboratory, Charleston, South Carolina, United States of America.
Robert W
RW
Chapman
Departments of Obstetrics and Gynecology, Medical University of South Carolina (MUSC), Charleston, South Carolina, United States of America.
Stephen E
SE
Somerville
Departments of Obstetrics and Gynecology, Medical University of South Carolina (MUSC), Charleston, South Carolina, United States of America.
Matthew P
MP
Guillette
Scientific Services, Mpumalanga Tourism and Parks Agency, Nelspruit, South Africa.|Department of Biodiversity, University of Limpopo, Sovenga, South Africa.
Hannes
H
Botha
Scientific Services, Mpumalanga Tourism and Parks Agency, Nelspruit, South Africa.
Andre
A
Hoffman
Department of Biodiversity, University of Limpopo, Sovenga, South Africa.
Wilmien J
WJ
Luus-Powell
Department of Biodiversity, University of Limpopo, Sovenga, South Africa.
Willem J
WJ
Smit
Department of Biodiversity, University of Limpopo, Sovenga, South Africa.
Jeffrey
J
Lebepe
Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Jan
J
Myburgh
Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.|Scientific Services, South African National Parks, Skukuza, South Africa.
Danny
D
Govender
Marine Resources Research Institute, South Carolina Department of Natural Resources, Hollings Marine Laboratory, Charleston, South Carolina, United States of America.
Jonathan
J
Tucker
National Institute of Standards and Technology (NIST), Material Measurement Laboratory, Chemical Sciences Division, Environmental Chemical Sciences Group, Hollings Marine Laboratory, Charleston, South Carolina, United States of America.
Ashley S P
AS
Boggs
Departments of Obstetrics and Gynecology, Medical University of South Carolina (MUSC), Charleston, South Carolina, United States of America.|Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Louis J
LJ
Guillette
D000818:Animals; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D001798:Blood Proteins / Q000032:analysis; D002118:Calcium / Q000097:blood; D005260:Female; D005393:Fish Diseases / Q000097:blood* / Q000175:diagnosis / Q000209:etiology; D029941:Fish Proteins / Q000097:blood; D008297:Male; D015434:Panniculitis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D045483:Rivers; D012709:Serum Albumin / Q000032:analysis; D012964:Sodium / Q000097:blood; D013019:South Africa; D013231:Steatitis / Q000097:blood* / Q000175:diagnosis / Q000209:etiology; D017210:Tilapia / Q000097:blood*; D014877:Water Pollution, Chemical / Q000009:adverse effects
D015415:Biomarkers; D001798:Blood Proteins; D029941:Fish Proteins; D012709:Serum Albumin; D012964:Sodium; D002118:Calcium