Yeast and mould dynamics in Caciofiore della Sibilla cheese coagulated with an aqueous extract of Carlina acanthifolia All.
Caciofiore della Sibilla is a speciality ewes' milk cheese traditionally manufactured in a foothill area of the Marche region (Central Italy) with a crude extract of fresh young leaves of Carlina acanthifolia All. subsp. acanthifolia as a coagulating agent. The fungal dynamics and diversity of this speciality cheese were investigated throughout the manufacturing and 20-day ripening process, using a combined PCR-DGGE approach. The fungal biota of a control ewes' milk cheese, manufactured with the same batch of milk coagulated with a commercial animal rennet, was also monitored by PCR-DGGE, in order to investigate the contribution of the peculiar vegetable coagulant to the fungal diversity and dynamics of the cheese. Based on the overall results collected, the raw milk and the dairy environment represented the main sources of fungal contamination, with a marginal or null contribution of thistle rennet to the fungal diversity and dynamics of Caciofiore della Sibilla cheese. Copyright © 2016 John Wiley & Sons, Ltd.
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Federica
F
Cardinali
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Manuela
0000-0003-0468-1045
M
Taccari
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Vesna
V
Milanović
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Andrea
A
Osimani
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Serena
S
Polverigiani
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Cristiana
C
Garofalo
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Roberta
R
Foligni
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Massimo
M
Mozzon
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Silvia
S
Zitti
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Nadia
N
Raffaelli
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Francesca
F
Clementi
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
Lucia
L
Aquilanti
D000818:Animals; D019659:Asteraceae / Q000201:enzymology* / Q000382:microbiology; D002470:Cell Survival; D002611:Cheese / Q000382:microbiology*; D012085:Chymosin / Q000737:chemistry*; D004271:DNA, Fungal / Q000235:genetics; D005516:Food Microbiology; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007558:Italy; D064307:Microbiota; D008892:Milk / Q000382:microbiology*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000201:enzymology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012756:Sheep; D013997:Time Factors
D004271:DNA, Fungal; D010936:Plant Extracts; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; C025350:rennet; D012085:Chymosin
Caciofiore della Sibilla; PCR-DGGE; mould; raw ewes' milk cheese; thistle rennet; yeast