Phage sensitivity and prophage carriage in Staphylococcus aureus isolated from foods in Spain and New Zealand.
Bacteriophages (phages) are a promising tool for the biocontrol of pathogenic bacteria, including those contaminating food products and causing infectious diseases. However, the success of phage preparations is limited by the host ranges of their constituent phages. The phage resistance/sensitivity profile of eighty seven Staphylococcus aureus strains isolated in Spain and New Zealand from dairy, meat and seafood sources was determined for six phages (Φ11, K, ΦH5, ΦA72, CAPSa1 and CAPSa3). Most of the S. aureus strains were sensitive to phage K (Myoviridae) and CAPSa1 (Siphoviridae) regardless of their origin. There was a higher sensitivity of New Zealand S. aureus strains to phages isolated from both Spain (ΦH5 and ΦA72) and New Zealand (CAPSa1 and CAPSa3). Spanish phages had a higher infectivity on S. aureus strains of Spanish dairy origin, while Spanish strains isolated from other environments were more sensitive to New Zealand phages. Lysogeny was more prevalent in Spanish S. aureus compared to New Zealand strains. A multiplex PCR reaction, which detected ΦH5 and ΦA72 sequences, indicated a high prevalence of these prophages in Spanish S. aureus strains, but were infrequently detected in New Zealand strains. Overall, the correlation between phage resistance and lysogeny in S. aureus strains was found to be weak.
Instituto de Productos Lácteos de Asturias (IPLA-CSIC). Paseo Río Linares s/n, 33300 Villaviciosa, Asturias, Spain.
Diana
D
Gutiérrez
Instituto de Productos Lácteos de Asturias (IPLA-CSIC). Paseo Río Linares s/n, 33300 Villaviciosa, Asturias, Spain.
Lorena
L
Rodríguez-Rubio
Instituto de Productos Lácteos de Asturias (IPLA-CSIC). Paseo Río Linares s/n, 33300 Villaviciosa, Asturias, Spain.
Pilar
P
García
Food, Water and Environmental Microbiology Group, Institute of Environmental Science and Research, Christchurch Science Centre, Christchurch 8041, New Zealand.
Craig
C
Billington
Food, Water and Environmental Microbiology Group, Institute of Environmental Science and Research, Christchurch Science Centre, Christchurch 8041, New Zealand.
Aruni
A
Premarante
Instituto de Productos Lácteos de Asturias (IPLA-CSIC). Paseo Río Linares s/n, 33300 Villaviciosa, Asturias, Spain.
Ana
A
Rodríguez
Instituto de Productos Lácteos de Asturias (IPLA-CSIC). Paseo Río Linares s/n, 33300 Villaviciosa, Asturias, Spain. Electronic address: bmf1@ipla.csic.es.
Beatriz
B
Martínez
D061046:Biological Control Agents; D058507:Host Specificity; D008242:Lysogeny; D017900:Myoviridae / Q000254:growth & development*; D009520:New Zealand; D039002:Prophages / Q000235:genetics / Q000254:growth & development*; D017906:Siphoviridae / Q000254:growth & development*; D013030:Spain; D013203:Staphylococcal Infections / Q000382:microbiology / Q000628:therapy; D013204:Staphylococcus Phages / Q000235:genetics / Q000254:growth & development*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000821:virology*
D061046:Biological Control Agents
Bacteriophage; Biocontrol; Lysogeny; Phage resistance; Staphylococcus aureus