Enrichment and immobilization of sulfide removal microbiota applied for environmental biological remediation of aquaculture area.
To remove sulfide in the deteriorating aquaculture sediment and water, sulfide-oxidizing microbiota was enriched from Jiaozhou Bay, China, by using sulfide-rich medium. Composition and structure of microbial communities in the enrichments were investigated by 16S rDNA molecular biotechniques. Results showed that microbial community structure continuously shifted and the abundance of sulfate reducing bacteria, i.e., Desulfobacterium, Desulfococcus and Desulfobacca apparently declined. Several halophile genera, Vibrio, Marinobacter, Pseudomonas, Prochlorococcus, Pediococcus and Thiobacillus predominated finally in the microbiota. The enriched microbiota was capable of removing a maximum of 1000 mg/L sulfide within 12 h with 10% inoculum at pH 7.0, 20-30 °C. After immobilized, the microbiota presented excellent resistance to impact and could completely remove 600 mg/L sulfide in 12 h. Moreover, the immobilized microbiota recovered well even recycled for five times. In conclusion, the immobilized sulfide-removing microbiota showed a quite promising application for biological restoring of sulfide-rich aquaculture environment.
Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering (MEGE), Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: ygzhao@ouc.edu.cn.
Yang-Guo
YG
Zhao
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Yu
Y
Zheng
Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Weijun
W
Tian
Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Jie
J
Bai
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Gong
G
Feng
Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering (MEGE), Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Liang
L
Guo
Key Laboratory of Marine Environment and Ecology (Ocean University of China), Ministry of Education, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering (MEGE), Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Mengchun
M
Gao
D017756:Aquaculture; D002681:China; D064307:Microbiota; D010084:Oxidation-Reduction; D013440:Sulfides / Q000378:metabolism*; D014871:Water Microbiology
D013440:Sulfides
Environmental bioremediation; Immobilization; Microbial community; Sulfide removal