High glucose-induced Matrilin-2 expression in mouse mesangial cells was mediated by transforming growth factor beta 1 (TGF-β1).
This study aimed at evaluating the effect of high glucose on the expression of extracellular matrix (ECM) protein Matrilin-2 and the mechanism underlying this effect by using a mouse mesangial cell line. Mouse mesangial cells (MMCs) were cultured in media containing normal (5 mM d-glucose) or high concentrations of glucose (30 mM d-glucose). The expression of Matrilin-2 was assessed by either RT-PCR or western blot. Additionally, transforming growth factor beta 1 (TGF-β1) inhibitors and TGF-β1 were used to determine whether glucose-regulated Matrilin-2 expression was mediated by the TGF-β1/Smad3 signaling pathway. Our data demonstrated that Matrilin-2 expression was markedly induced by high glucose and TGF-β1. High glucose-induced Matrilin-2 expression was inhibited by TGF-β1/Smad3 inhibitors, indicating that Matrilin-2 was markedly induced by high glucose and this induction was mediated by the TGF-β1/Smad3 pathway. Taken together, our results showed that high-glucose-induced Matrilin-2 expression that was mediated by the TGF-β1/Smad3 signaling pathway might play a role in Diabetic nephropathy (DN) pathogenesis and our finding provided a potential diagnostic and/or therapeutic target for DN.
Department of Pathology, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, Qinghai 810007, China.
Shukun
S
Zhang
Department of Pathology, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, Qinghai 810007, China.
Menglan
M
Zhang
Medical College of Qinghai University, 16 Kunlun Road, Xining 810000, China.
Hong
H
Huang
Department of Pathology, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, Qinghai 810007, China.
Shiying
S
Zhou
Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Yanshneg
Y
Du
Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address: xinyi@iupui.edu.
Xin
X
Yi
Department of Pathology, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, Qinghai 810007, China. Electronic address: jluo099@163.com.
Junming
J
Luo
D000818:Animals; D002460:Cell Line; D005947:Glucose / Q000008:administration & dosage* / Q000378:metabolism*; D064235:Matrilin Proteins / Q000378:metabolism*; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D051900:Smad3 Protein / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D064235:Matrilin Proteins; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D053773:Transforming Growth Factor beta1; D005947:Glucose
Diabetic nephropathy; Extracellular matrix; Matrilin-2; Transforming growth factor beta 1