Rapid method for DNA isolation from a tough cell wall green alga Tetraspora sp. CU2551.
Genetic studies are important to understand the complex biological system of various organisms. Some eukaryotic green organisms have tough cell wall which precludes the efficient extraction of the genetic materials. Here, we developed the method for simple and rapid isolation of high quality DNA from a green alga Tetraspora sp. CU2551. The cell homogenization procedures were combined with physical force plus heat treatment to disrupt the cell envelope of Tetraspora sp. CU2551. Without protease treatment, vortexing with glass bead for 30-105 s at 70 °C led to the isolation of a high purity DNA which was suitable for downstream process. The improved method was successfully developed and could be applied for the rapid isolation of DNA from other unicellular and filamentous green microalgal strains.
Bioenergy Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.|Department of Chemistry, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Cherdsak
C
Maneeruttanarungroj
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. aran.i@chula.ac.th.
Aran
A
Incharoensakdi
D002473:Cell Wall / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry* / Q000166:cytology / Q000235:genetics*; D018744:DNA, Plant / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005898:Glass; D007202:Indicators and Reagents; D019800:Phenol / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
D018744:DNA, Plant; D007202:Indicators and Reagents; D019800:Phenol
Green algae; Rapid DNA isolation; Tetraspora