Lignans and triterpenoids from Vitex negundo var. heterophylla and their biological evaluation.
Three new phenylnaphthalene-type lignans, vitexnegheteroins E-G (1-3), and a new polyoxygenated ursane-type triterpene, vitexnegheteroin H (9), were isolated from the seeds of Vitex negundo var. heterophylla, together with ten known compounds. Their structures were established on the basis of extensive 1D and 2D NMR experiments, as well as their mass spectroscopic data. The absolute configurations of compounds 1 and 2 were determined by comparing their experimental ECD spectra with that calculated by the time-dependent density functional theory (TDDFT) method. The isolates were evaluated for their cytotoxicities against three human cancer cell lines, inhibitory activities on lipopolysaccharide-induced NO production in murine microglial BV-2 cells, and antioxidant activities for ABTS radical scavenging.
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Ping
P
Hu
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Da-Hong
DH
Li
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Xu
X
Hu
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Sheng-Ge
SG
Li
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Chun-Mei
CM
Sai
College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, People's Republic of China.
Xiao-Cong
XC
Sun
College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Tong
T
Su
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Jiao
J
Bai
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
Zi-Hou
ZH
Wang
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address: lzl1030@hotmail.com.
Zhan-Lin
ZL
Li
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address: huimhua@163.com.
Hui-Ming
HM
Hua
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification; D051379:Mice; D017628:Microglia / Q000187:drug effects; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification; D020950:Vitex / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D017705:Lignans; D014315:Triterpenes; D009569:Nitric Oxide
Anti-inflammation; Antioxidation; Cytotoxicity; Lignan; Triterpene; Vitex negundo var. heterophylla