Identification, expression analysis, and functional characterization of peptide YY in chickens (Gallus gallus domesticus).
Peptide YY (PYY) functions as a postprandial satiety signal in mammals. However, the genomic information and physiological roles of chicken PYY have not yet been clarified, although PYY peptide was isolated from chicken intestines in 1992. In this study, we identified a full-length complementary DNA (cDNA) sequence encoding the chicken PYY precursor. The deduced amino acid sequence of chicken PYY was completely consistent with the previously identified peptide sequence. PYY mRNA was abundantly expressed in the small intestine compared with the large intestine. PYY mRNA levels in the jejunum were significantly higher during ad libitum feeding compared with fasting, suggesting that intestinal PYY expression is altered in response to nutritional status in chicks. Intravenous administration of PYY significantly suppressed food intake in chicks. Furthermore, neuropeptide Y receptor Y2, a possible target of PYY, was expressed in various brain regions including the appetite-regulating centers in chicks. This is the first evidence that the intestinal hormone PYY may function as an anorexigenic hormone in chicks.
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Koji
K
Aoki
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Makoto
M
Kondo
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Mika
M
Okuda
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Takaoki
T
Saneyasu
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan. Electronic address: honda@tiger.kobe-u.ac.jp.
Kazuhisa
K
Honda
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Hiroshi
H
Kamisoyama
D061605:Administration, Intravenous; D000595:Amino Acid Sequence; D000818:Animals; D001066:Appetite / Q000502:physiology; D001921:Brain / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D018076:DNA, Complementary; D004435:Eating / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000502:physiology; D007583:Jejunum / Q000378:metabolism; D019894:Peptide YY / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012528:Satiety Response
D018076:DNA, Complementary; D012333:RNA, Messenger; D019894:Peptide YY
Appetite; Chicken; Food intake; Peptide YY