Expression of genes involved in lipid droplet formation (BSCL2, SNAP23 and COPA) during porcine in vitro adipogenesis.
Adipogenesis is a complex process of fat cells development driven by the expression of numerous genes. Differentiation of progenitor cells into mature adipocytes is accompanied by changes in cell shape, as a result of lipid accumulation. In the present study, expression of three genes involved in lipid droplet formation (SNAP23, BSCL2 and COPA) was evaluated during porcine adipogenesis. It was found that mRNA levels of BSCL2 and SNAP23, but not COPA, increased during differentiation. Redistribution of SNAP23 protein to different cellular compartments was observed when comparing undifferentiated mesenchymal stem cells and differentiated adipocytes. The BSCL2 protein was found to be highly specific to cells with accumulated lipids, while COPA protein coated the lipid droplets. Obtained results indicated that the studied genes may be considered as candidates for fatness traits in pigs. Moreover, this study has shown that the porcine in vitro adipogenesis system provides a useful tool for the characterisation of novel genes involved in adipose tissue accumulation.
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60-637, Poznan, Poland.
Beata
B
Kociucka
Chair of Livestock Biotechnology, Technische Universität München, München, Germany.
Tatiana
T
Flisikowska
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60-637, Poznan, Poland.
Dariusz
D
Mróz
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60-637, Poznan, Poland. izabel@jay.au.poznan.pl.
Izabela
I
Szczerbal
D017667:Adipocytes / Q000502:physiology*; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D020756:Coatomer Protein / Q000235:genetics*; D044388:GTP-Binding Protein gamma Subunits / Q000235:genetics*; D066292:Lipid Droplets / Q000502:physiology*; D050766:Qb-SNARE Proteins / Q000235:genetics*; D050767:Qc-SNARE Proteins / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D020756:Coatomer Protein; D044388:GTP-Binding Protein gamma Subunits; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins
Adipocyte; Fatness; Mesenchymal stem cells; Model organism; Obesity; Pig