Self-Assembled Enzyme Nanoparticles for Carbon Dioxide Capture.
Enzyme-based processes have shown promise as a sustainable alternative to amine-based processes for carbon dioxide capture. In this work, we have engineered carbonic anhydrase nanoparticles that retain 98% of hydratase activity in comparison to their free counterparts. Carbonic anhydrase was fused with a self-assembling peptide that facilitates the noncovalent assembly of the particle and together were recombinantly expressed from a single gene construct in Escherichia coli. The purified enzymes, when subjected to a reduced pH, form 50-200 nm nanoparticles. The CO2 capture capability of enzyme nanoparticles was demonstrated at ambient (22 ± 2 °C) and higher (50 °C) temperatures, under which the nanoparticles maintain their assembled state. The carrier-free enzymatic nanoparticles demonstrated here offer a new approach to stabilize and reuse enzymes in a simple and cost-effective manner.
Department of Chemical Engineering, Monash University , Wellington Road, Clayton, Victoria 3800, Australia.
Bhuvana Kamath
BK
Shanbhag
Department of Mechanical and Aerospace Engineering, Monash University , Wellington Road, Clayton, Victoria 3800, Australia.
Boyin
B
Liu
Department of Mechanical and Aerospace Engineering, Monash University , Wellington Road, Clayton, Victoria 3800, Australia.
Jing
J
Fu
Department of Chemical Engineering, Monash University , Wellington Road, Clayton, Victoria 3800, Australia.
Victoria S
VS
Haritos
Department of Chemical Engineering, Monash University , Wellington Road, Clayton, Victoria 3800, Australia.
Lizhong
L
He
D000327:Adsorption; D002245:Carbon Dioxide / Q000737:chemistry*; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011485:Protein Binding; D013696:Temperature
D002245:Carbon Dioxide; D002256:Carbonic Anhydrases
carbon dioxide capture; carrier-free immobilization; enzyme-peptide fusion; protein nanoparticles; recombinant enzyme; self-assembly