Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.
Differentiation of preadipocyte, also called adipogenesis, leads to the phenotype of mature adipocyte. However, excessive adipogenesis is closely linked to the development of obesity. Artesunate, one of artemisinin-type sesquiterpene lactones from Artemisia annua L., is known for anti-malarial and anti-cancerous activities. In this study, we investigated the effect of artesunate on adipogenesis in 3T3-L1 preadipocytes. Artesunate strongly inhibited lipid accumulation and triglyceride (TG) synthesis during the differentiation of 3T3-L1 preadipocytes into adipocytes at 5 μM concentration. Artesunate at 5 μM also reduced not only the expressions of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3 (STAT-3) during adipocyte differentiation. Moreover, artesunate at 5 μM reduced leptin, but not adiponectin, mRNA expression during adipocyte differentiation. Taken together, these findings demonstrate that artesunate inhibits adipogenesis in 3T3-L1 preadipoytes through the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.
Department of Molecular Medicine, College of Medicine, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, South Korea. Electronic address: jangbc123@gw.kmu.ac.kr.
Byeong-Churl
BC
Jang
D016475:3T3 Cells; D050156:Adipogenesis / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D037621:Artemisinins / Q000008:administration & dosage*; D000077332:Artesunate; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D000070778:Perilipin-1 / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
D037621:Artemisinins; D022763:CCAAT-Enhancer-Binding Protein-alpha; C491899:Fas protein, mouse; D047495:PPAR gamma; D000070778:Perilipin-1; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D019014:fas Receptor; D000077332:Artesunate
Adipogenesis; Artesunate; C/EBP-α; FAS; PPAR-γ; STAT-3