Potentials of single-cell biology in identification and validation of disease biomarkers.
Single-cell biology is considered a new approach to identify and validate disease-specific biomarkers. However, the concern raised by clinicians is how to apply single-cell measurements for clinical practice, translate the message of single-cell systems biology into clinical phenotype or explain alterations of single-cell gene sequencing and function in patient response to therapies. This study is to address the importance and necessity of single-cell gene sequencing in the identification and development of disease-specific biomarkers, the definition and significance of single-cell biology and single-cell systems biology in the understanding of single-cell full picture, the development and establishment of whole-cell models in the validation of targeted biological function and the figure and meaning of single-molecule imaging in single cell to trace intra-single-cell molecule expression, signal, interaction and location. We headline the important role of single-cell biology in the discovery and development of disease-specific biomarkers with a special emphasis on understanding single-cell biological functions, e.g. mechanical phenotypes, single-cell biology, heterogeneity and organization of genome function. We have reason to believe that such multi-dimensional, multi-layer, multi-crossing and stereoscopic single-cell biology definitely benefits the discovery and development of disease-specific biomarkers.
Huzhou Central Hospital, Huzhou, Zhejiang Province, China.
Furong
F
Niu
Department of Pulmonary Medicine, The First affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
Diane C
DC
Wang
Department of Pulmonary Medicine, The First affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
Jiapei
J
Lu
Huzhou Central Hospital, Huzhou, Zhejiang Province, China.
Wei
W
Wu
Huzhou Central Hospital, Huzhou, Zhejiang Province, China.
Xiangdong
X
Wang
D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D004194:Disease; D006801:Humans; D015203:Reproducibility of Results; D017421:Sequence Analysis; D059010:Single-Cell Analysis; D049490:Systems Biology
D015415:Biomarkers
gene sequencing; genome function; heterogeneity; mechanical phenotypes; single-cell biology