Diversity in cell motility reveals the dynamic nature of the formation of zebrafish taste sensory organs.
Taste buds are sensory organs in jawed vertebrates, composed of distinct cell types that detect and transduce specific taste qualities. Taste bud cells differentiate from oropharyngeal epithelial progenitors, which are localized mainly in proximity to the forming organs. Despite recent progress in elucidating the molecular interactions required for taste bud cell development and function, the cell behavior underlying the organ assembly is poorly defined. Here, we used time-lapse imaging to observe the formation of taste buds in live zebrafish larvae. We found that tg(fgf8a.dr17)-expressing cells form taste buds and get rearranged within the forming organs. In addition, differentiating cells move from the epithelium to the forming organs and can be displaced between developing organs. During organ formation, tg(fgf8a.dr17) and type II taste bud cells are displaced in random, directed or confined mode relative to the taste bud they join or by which they are maintained. Finally, ascl1a activity in the 5-HT/type III cell is required to direct and maintain tg(fgf8a.dr17)-expressing cells into the taste bud. We propose that diversity in displacement modes of differentiating cells acts as a key mechanism for the highly dynamic process of taste bud assembly.
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France.
Marina
M
Soulika
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France.
Anna-Lila
AL
Kaushik
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France.
Benjamin
B
Mathieu
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France.
Raquel
R
Lourenço
Developmental Neurobiology and Genomics, Brain and Mind Research Institute, University of Sydney, Camperdown, NSW 2050, Australia.
Anna Z
AZ
Komisarczuk
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France.
Sebastian Alejo
SA
Romano
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France.
Adrien
A
Jouary
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France.
Alicia
A
Lardennois
Institut Jacques Monod, CNRS UMR7592, University Paris Diderot, 75013 Paris, France.
Nicolas
N
Tissot
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Shinji
S
Okada
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Keiko
K
Abe
Developmental Neurobiology and Genomics, Brain and Mind Research Institute, University of Sydney, Camperdown, NSW 2050, Australia.
Thomas S
TS
Becker
IBENS, Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, 75230 Paris, France marika.kapsimali@ens.fr.
Marika
0000-0001-9327-5166
M
Kapsimali
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D004742:Enhancer Elements, Genetic / Q000235:genetics; D007814:Larva / Q000166:cytology / Q000378:metabolism; D038081:Organogenesis; D012701:Serotonin / Q000378:metabolism; D013650:Taste Buds / Q000166:cytology* / Q000254:growth & development*; D014157:Transcription Factors; D015027:Zebrafish / Q000502:physiology*; D029961:Zebrafish Proteins / Q000378:metabolism
D051792:Basic Helix-Loop-Helix Proteins; D012701:Serotonin; D014157:Transcription Factors; D029961:Zebrafish Proteins; C468127:Ascl1a protein, zebrafish
Ascl1a; MSD; Taste bud; Taste receptor cell; Time-lapse; fgf8a