Genome-wide identification and expression profiling of the C2H2-type zinc finger protein transcription factor family in tobacco.
C2H2 zinc finger protein transcription factor family members have important biological functions in eukaryotes. They not only bind DNA and RNA, but also interact with proteins. In this study, 118 members of the tobacco C2H2 zinc finger protein transcription factor family were identified from the N. tabacum genome database by using Pfam, SMART and Blastp. The analyses of phylogenetic tree, physical and chemical properties, chromosomal mapping, gene structures, protein three-dimensional structures and tissue expression patterns were performed. The results suggested that the peptide length of different subfamily members is significantly different. Phylogenetic and motif analysis revealed that the C2H2 zinc finger protein transcription factor family members can be divided into 5 subfamilies and each member has at least one C2H2 motif. Genes of the family members are distributed across the 22 chromosomes. C2H2 zinc finger protein transcription factor family members are expressed in different tissues although some have higher expression levels in leaves and roots. This study will be helpful for further analysis of the C2H2 zinc finger family proteins in other plants.
Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China; Graduate School of Chinese Academy of Agricultural Science, Beijing 100081, Chinbr/a;
Ming-lei
ML
Yang
Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China.
Jiang-tao
JT
Chao
Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China.
Da-wei
DW
Wang
Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China;Graduate School of Chinese Academy of Agricultural Science, Beijing 100081, Chinbr/a;
Jun-hua
JH
Hu
Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China; Graduate School of Chinese Academy of Agricultural Science, Beijing 100081, Chinbr/a;
Hua
H
Wu
Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China.
Daping
D
Gong
Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China.
Guan-shan
GS
Liu
D000595:Amino Acid Sequence; D020869:Gene Expression Profiling; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D010940:Plant Proteins; D014157:Transcription Factors