The genetic diversity of SMLS (Sitobion miscanthi L type symbiont) and its effect on the fitness, mitochondrial DNA diversity and Buchnera aphidicola dynamic of wheat aphid, Sitobion miscanthi (Hemiptera: Aphididae).
SMLS (Sitobion miscanthi L type symbiont) is a recently discovered aphid secondary symbiont. Using evidence extracted from 16S rRNA sequences, previous studies indicate that SMLS is the most widely distributed and most recently transferred secondary symbiont in Chinese Sitobion miscanthi populations. Here, we further investigated genetic diversity among SMLS geographic strains with multiloci data. Furthermore, the influence of SMLS on S. miscanthi was uncovered with ecological and evolutionary evidence. The results indicated that there was limited influence of infection with SMLS on variation and evolutionary patterns of S. miscanthi mitochondrial DNA. By hemolymph injection, the SMLS-infected and SMLS-uninfected S. miscanthi clones with the identical genetic background were built in this study. Although similar Buchnera aphidicola dynamics were observed between SMLS-infected and SMLS-uninfected S. miscanthi population, B. aphidicola density of SMLS-infected S. miscanthi population was always significantly higher than SMLS-uninfected ones. The results of fitness measurements indicated that under laboratory rearing conditions, transfection of SMLS could confer modest advantages to some fitness components of S. miscanthi, that is, total number of offspring, longevity, age of first reproduction and weight of adult. However, as SMLS is not strictly associated with S. miscanthi, further investigations are needed to uncover the mechanisms responsible for this inconceivable association.
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Tong
T
Li
Institute of Quality Standard and Testing Technology for Agro-products/Henan Key Laboratory of Grain Quality and Safety and Testing/Laboratory of Quality Safety Risk Assessment for Agro-products, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Xu-Jin
XJ
Wu
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Yue-Li
YL
Jiang
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Li
L
Zhang
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Yun
Y
Duan
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Jin
J
Miao
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Zhong-Jun
ZJ
Gong
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou, 450002, China.
Yu-Qing
YQ
Wu
D000818:Animals; D001042:Aphids / Q000235:genetics* / Q000382:microbiology*; D005075:Biological Evolution; D020635:Buchnera / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D005798:Genes, Bacterial; D056084:Genetic Fitness; D014644:Genetic Variation; D005828:Genetics, Population; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S
Buchnera aphidicola; aphid; mitochondrial DNA; secondary symbiont