A TiS2 nanosheet enhanced fluorescence polarization biosensor for ultra-sensitive detection of biomolecules.
Development of new strategies for the sensitive and selective detection of ultra-low concentrations of specific cancer markers is of great importance for assessing cancer therapeutics due to its crucial role in early clinical diagnoses and biomedical applications. In this work, we have developed two types of fluorescence polarization (FP) amplification assay strategies for the detection of biomolecules by using TiS2 as a FP enhancer and Zn(2+)-dependent self-hydrolyzing deoxyribozymes as catalysts to realize enzyme-catalyzed target-recycling signal amplification. One approach is based on the terminal protection of small-molecule-linked DNA, in which biomolecular binding to small molecules in DNA-small-molecule chimeras can protect the conjugated DNA from degradation by exonuclease I (Exo I); the other approach is based on the terminal protection of biomolecular bound aptamer DNA, in which biomolecules directly bound to the single strand aptamer DNA can protect the ssDNA from degradation by Exo I. We select folate receptor (FR) and thrombin (Tb) as model analytes to verify the current concept. It is shown that under optimized conditions, our strategies exhibit high sensitivity and selectivity for the quantification of FR and Tb with low detection limits (0.003 ng mL(-1) and 0.01 pM, respectively). Additionally, this strategy is a simple "mix and detect" approach, and does not require any separation steps. This biosensor is also utilized in the analysis of real biological samples, the results agree well with those obtained by the enzyme-linked immunosorbent assay (ELISA).
School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, P. R. China. fanjing@htu.cn.
Xiang
X
Li
Department of Chemistry, Sanquan Medical College, Xinxiang Medical University, Xinxiang, Henan 453003, P. R. China.
Xuelian
X
Ding
Life Science College, Henan Normal University, Xinxiang, Henan, 453007, P. R. China.
Yongfang
Y
Li
Life Science College, Henan Normal University, Xinxiang, Henan, 453007, P. R. China.
Linsong
L
Wang
School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, P. R. China. fanjing@htu.cn.
Jing
J
Fan
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005454:Fluorescence Polarization; D058975:Folate Receptor 1 / Q000032:analysis; D057230:Limit of Detection; D013917:Thrombin / Q000032:analysis
D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D058975:Folate Receptor 1; D013917:Thrombin