Purification, characterization and antioxidant activity of polysaccharides from Flammulina velutipes residue.
In this study, we isolated polysaccharides from Flammulina velutipes residue (FVRP) using microwave-assisted extraction and then purified the polysaccharides by column chromatography to yield FVRP-1, FVRP-2 and FVRP-3. The structural characteristics of FVRP-1, FVRP-2 and FVRP-3 were investigated, and their antioxidant activities against ABTS(+), DPPH and hydroxyl radicals were also analyzed in vitro. FVRP-1 was found to be neutral and rich in galactose. However, FVRP-2 and FVRP-3 were acidic polysaccharides and were rich in glucose. The average molecular weight of FVRP-1, FVRP-2 and FVRP-3 were 29,930, 62,290, and 36,310Da, respectively. The glycosyl residue of FVRP-1 was an α-type glycosidic linkage, whereas FVRP-2 and FVRP-3 were β-type glycosidic linkages. We found FVRP-1, FVRP-2 and FVRP-3 had strong potential antioxidant activities in the order of FVRP-1<FVRP-3<FVRP-2. Our results thus suggested that FVRPs might be suitable for use as functional foods and as potential therapeutic agents.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Ying
Y
Liu
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Bin
B
Zhang
Department of Family and Consumer Sciences, North Carolina A&T State University, 171 Carver Hall, Greensboro, NC 27411, United States.
S A
SA
Ibrahim
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Shuang-Shuang
SS
Gao
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Hong
H
Yang
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: huangwen@mail.hzau.edu.cn.
Wen
W
Huang
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D055393:Flammulina; D019649:Food Industry; D017665:Hydroxyl Radical / Q000737:chemistry; D007220:Industrial Waste; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013451:Sulfonic Acids / Q000737:chemistry
D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D007220:Industrial Waste; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
Antioxidant activity; Characterization; Flammulina velutipes residue; Polysaccharide; Purification