Selenoprotein M gene expression, peroxidases activity and hydrogen peroxide concentration are differentially regulated in gill and hepatopancreas of the white shrimp Litopenaeus vannamei during hypoxia and reoxygenation.
In many organisms, episodes of low O2 concentration (hypoxia) and the subsequent rise of O2 concentration (reoxygenation) result in the accumulation of reactive oxygen species and oxidative stress. Selenoprotein M (SelM), is a selenocysteine containing protein with redox activity involved in the antioxidant response. It was previously shown that in the white shrimp Litopenaeus vannamei, the silencing of SelM by RNAi decreased peroxidase activity in gill. In this work, we report the structure of the SelM gene (LvSelM) and its relative expression in hepatopancreas and gill after 24h of hypoxia followed by 1h of reoxygenation. The gene is composed by four exons interrupted by tree introns. In gills and hepatopancreas, SelM expression increased after 24h of hypoxia followed by 1h of reoxygenation, while peroxidases activity diminished in hepatopancreas but increased in gills. Hydrogen peroxide (H2O2) concentration was higher in hepatopancreas in response to hypoxia for 6h and did not change after 24 of hypoxia followed by reoxygenation; conversely, no change was detected in gill. SelM appears to be a key enzyme in gill oxidative stress regulation, since the higher expression is associated with an increase in peroxidases activity while maintaining H2O2 concentration. In contrast, in hepatopancreas there is a higher expression after hypoxia and reoxygenation for 24h, but peroxidases activity was lower and the change in H2O2 occurred after 6h of hypoxia and this level was maintained during reoxygenation.
Universidad Autónoma de Chihuahua, Facultad de Ciencias Química, Laboratorio de Biología Molecular, Chihuahua, Mexico.
Antonio
A
García-Triana
Centro de Investigación en Alimentación y Desarrollo (CIAD), Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico.
Alma Beatriz
AB
Peregrino-Uriarte
Centro de Investigación en Alimentación y Desarrollo (CIAD), Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico. Electronic address: gyepiz@ciad.mx.
Gloria
G
Yepiz-Plascencia
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015870:Gene Expression; D005880:Gills / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D000860:Hypoxia; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D017382:Reactive Oxygen Species; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051140:Selenoproteins / Q000235:genetics*
D060829:Arthropod Proteins; D017382:Reactive Oxygen Species; D051140:Selenoproteins; D006861:Hydrogen Peroxide; D010544:Peroxidases; D010100:Oxygen
Hydrogen peroxide; Hypoxia; Peroxidases; Reoxygenation; Selenoprotein M (SelM); Shrimp