miR-17, miR-21, and miR-143 Enhance Adipogenic Differentiation from Porcine Bone Marrow-Derived Mesenchymal Stem Cells.
Bone marrow-derived mesenchymal stem cells (BMSCs) have multilineage differentiation abilities toward adipocytes and osteoblasts. Recently, numerous studies have focused on the roles of microRNAs (miRNAs) in the process of adipogenic differentiation of human and mouse cells. However, the role of miRNAs in adipogenic differentiation process of porcine BMSCs (pBMSCs) remains unclear. In this study, pBMSCs were induced to differentiate into adipocytes using a chemical approach, and the roles of miR-17, miR-21, and miR-143 in this process were investigated. Our results showed that pBMSCs could be chemically induced to differentiate into adipocytes and that the expression of miR-17, miR-21, and miR-143 increased during differentiation. Then, overexpression of mimics of miR-17, miR-21, and miR-143 increased the number of oil red O-positive cells of adipocyte differentiation. The expression levels of CCAAT/enhancer-binding protein alpha (C/EBPα) mRNA showed increases of 1.8-, 1.5-, and 1.2-fold in the groups expressing mimics of miR-21, miR-17, and miR-143, respectively, at day 20. These results demonstrate that miR-17, miR-21, and miR-143 are involved in and promote the adipogenic differentiation of pBMSCs. This study provides an experimental basis for establishing a stable and efficient adipogenic differentiation model for applications in cell therapy and tissue engineering.
1 College of Animal Science, Jilin University , Changchun, China .|2 State & Local Joint Engineering Laboratory for Animal Models of Human Diseases, Academy of Translational Medicine, First Hospital, Jilin University , Changchun, China .
Xinglan
X
An
1 College of Animal Science, Jilin University , Changchun, China .
Kuiying
K
Ma
1 College of Animal Science, Jilin University , Changchun, China .
Zhiren
Z
Zhang
3 College of Veterinary Medicine, Jilin University , Changchun, China .
Tianchuang
T
Zhao
3 College of Veterinary Medicine, Jilin University , Changchun, China .
Xueming
X
Zhang
3 College of Veterinary Medicine, Jilin University , Changchun, China .
Bo
B
Tang
1 College of Animal Science, Jilin University , Changchun, China .|2 State & Local Joint Engineering Laboratory for Animal Models of Human Diseases, Academy of Translational Medicine, First Hospital, Jilin University , Changchun, China .
Ziyi
Z
Li
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018716:Molecular Mimicry; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D013552:Swine
D022762:CCAAT-Enhancer-Binding Proteins; D007328:Insulin; D035683:MicroRNAs; D009843:Oligoribonucleotides; D003907:Dexamethasone; D007213:Indomethacin