CpG Oligodeoxynucleotides Downregulate Placental Adiponectin and Increase Embryo Loss in Non-Obese Diabetic Mice.
CpG oligodeoxynucleotides (ODNs) can induce immunological changes in non-obese diabetic (NOD) mice and increase embryo loss, but little is known about the mechanism. This study aimed to determine the role of adiponectin in CpG ODN-induced pregnancy failure.



Oligodeoxynucleotide 1826 was intraperitoneally injected to NOD mice, and ODN 2216, ODN 2006, and ODN 2395 were used to stimulate human trophoblast cell lines to investigate adiponectin expression patterns and its possible effects on trophoblast function.



CpG ODNs downregulated adiponectin via the cJun N-terminal kinase signaling pathway and led to increased embryo loss (from 6.9 to 33.3%). ODN 2006 impaired human trophoblast cell migration, which was successfully rescued by adiponectin treatment.



CpG ODNs decreased placental adiponectin expression in NOD mice and impaired human trophoblast function and was associated with increased embryo loss. Adiponectin may therefore play an important protective role in the prevention of bacteria-induced pregnancy failure.
Institute of Embryo-Fetal Original Adult Disease, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Chuan-Mei
CM
Qin
Institute of Embryo-Fetal Original Adult Disease, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Fu-Ju
FJ
Tian
Institute of Embryo-Fetal Original Adult Disease, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xiao-Rui
XR
Liu
Institute of Embryo-Fetal Original Adult Disease, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Fan
F
Wu
Institute of Embryo-Fetal Original Adult Disease, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xiao-Ling
XL
Ma
Institute of Embryo-Fetal Original Adult Disease, The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yi
Y
Lin
D052242:Adiponectin / Q000276:immunology*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects* / Q000276:immunology; D020964:Embryo Loss / Q000139:chemically induced / Q000276:immunology; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D009838:Oligodeoxyribonucleotides / Q000009:adverse effects* / Q000494:pharmacology; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy
C499918:ADIPOQ protein, human; D052242:Adiponectin; C499919:Adipoq protein, mouse; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides
Adipokine; immunodeficiency; infection; trophoblast