Heat treatment of curdlan enhances the enzymatic production of biologically active β-(1,3)-glucan oligosaccharides.
Biologically active β-(1,3)-glucan oligosaccharides were prepared from curdlan using GH64 enzyme (KfGH64). KfGH64 showed low activity toward native curdlan; thereby pretreatment conditions of curdlan were evaluated. KfGH64 showed the highest activity toward curdlan with heat treatment. The most efficient pretreatment (90°C for 0.5h) converted approximately 60% of curdlan into soluble saccharides under the optimized enzyme reaction conditions (pH 5.5, 37°C, 100rpm mixing speed, 24h, and 10μg of KfGH64/1g of curdlan). The resulting products were predominantly laminaripentaose and a small amount of β-(1,3)-glucans with an average degree of polymerization (DP) of 13 and 130. The products did not contain small oligosaccharides (DP<5), indicating that the hydrolysis of heat-treated curdlan by KfGH64 is a suitable method for the production of biologically active β-(1,3)-glucan oligosaccharides.
Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan; Research Fellow of the Japan Society for the Promotion of Science (JSPS), 8 Ichibancho, Chiyoda-ku, Tokyo 102-8472, Japan.
Yuya
Y
Kumagai
Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
Masayuki
M
Okuyama
Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan. Electronic address: kimura@abs.agr.hokudai.ac.jp.
Atsuo
A
Kimura
D039903:Actinobacteria / Q000201:enzymology; D001426:Bacterial Proteins / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006358:Hot Temperature; D006868:Hydrolysis; D009844:Oligosaccharides / Q000378:metabolism*; D012995:Solubility; D047071:beta-Glucans / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D009844:Oligosaccharides; D047071:beta-Glucans; C038459:curdlan; D006026:Glycoside Hydrolases
Curdlan; GH64; β-(1,3)-glucan oligosaccharides