Preparing Samples for Crystallization of Bcl-2 Family Complexes.
High-resolution protein structures determined by X-ray crystallography or NMR have proven invaluable for deciphering the molecular mechanisms underlying the function of a vast range of proteins. Here, we describe methods to generate complexes of proteins belonging to the Bcl-2 family of proteins with either biological ligands or small molecule antagonists.
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Physical Sciences 4, Room 205, Kingsbury Drive, Bundoora, Melbourne, VIC, 3086, Australia.|La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.
Marc
M
Kvansakul
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia. czabotar@wehi.edu.au.|Department of Medical Biology, The University of Melbourne, Melbourne, VIC, 3052, Australia. czabotar@wehi.edu.au.
Peter E
PE
Czabotar
D001713:Biphenyl Compounds / Q000378:metabolism; D018360:Crystallography, X-Ray / Q000379:methods*; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D009596:Nitrophenols / Q000378:metabolism; D010879:Piperazines / Q000378:metabolism; D011487:Protein Conformation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry* / Q000378:metabolism; D013048:Specimen Handling / Q000379:methods*; D013449:Sulfonamides / Q000378:metabolism
C501332:ABT-737; D001713:Biphenyl Compounds; D008024:Ligands; D009596:Nitrophenols; D010879:Piperazines; D019253:Proto-Oncogene Proteins c-bcl-2; D013449:Sulfonamides
Bcl-2 family proteins; Drug discovery; Structural biology; X-ray crystallography