Plasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth.
RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG) was successfully synthetized and then used to deliver plasmid-based Stat3 siRNA. The carrier can readily bind plasmid with high transfection efficiency. Moreover, molecular biology studies reveal that Stat3-related gene and protein expressions were significantly inhibited, suggesting that the formation of GO-PEI-PEG complexes could be utilized as a promising gene delivery in cancer therapy.
Prostate Diseases Prevention and Treatment Research Centre and Department of Pathophysiology, Norman Bethune Medical School, Jilin University, Changchun, China.
Di
D
Yin
Yang
Y
Li
Baofeng
B
Guo
Zhewen
Z
Liu
Yang
Y
Xu
Xiaoqin
X
Wang
Yanwei
Y
Du
Libo
L
Xu
Yan
Y
Meng
Xuejian
X
Zhao
Ling
L
Zhang
D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy / Q000379:methods*; D006108:Graphite / Q000008:administration & dosage / Q000737:chemistry; D008546:Melanoma, Experimental / Q000235:genetics* / Q000628:therapy* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry; D010087:Oxides / Q000008:administration & dosage / Q000737:chemistry; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D011897:Random Allocation; D050796:STAT3 Transcription Factor / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D010087:Oxides; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D011092:Polyethylene Glycols; D006108:Graphite