Hydrodynamic Injection as a Method of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection.
Gene delivery methods are important for both therapeutic intervention and as tools in research to address specific questions. Hydrodynamic injection (HDI) is a method that facilitates the delivery and expression of genetic material in target cells, namely hepatocytes, through an intravenous injection. HDI has great utility for research involving cell death and signaling pathways essential in the processes of cancer, inflammation, and transplant therapy, as well as representing a valuable technique to establish hepatitis B virus (HBV) expression in hepatocytes. This chapter describes in detail how to generate a model of chronic HBV infection in immunocompetent mice using HDI as a delivery method.
The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.|Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia.
Simon P
SP
Preston
The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.|Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia.
Marc
M
Pellegrini
The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia. ebert@wehi.edu.au.|Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia. ebert@wehi.edu.au.
Gregor
G
Ebert
D000818:Animals; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D016679:Genome, Viral; D006515:Hepatitis B virus / Q000472:pathogenicity*; D019694:Hepatitis B, Chronic / Q000821:virology*; D057446:Hydrodynamics; D007267:Injections / Q000379:methods*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010957:Plasmids
Gene delivery; HBV; Hydrodynamic injection; In vivo transfection