Blastomere removal from cleavage-stage mouse embryos alters placental function, which is associated with placental oxidative stress and inflammation.
Blastomere biopsy is an essential technique in preimplantation genetic diagnosis (PGD), a screening test that can detect genetic abnormalities of embryos before their transfer into uterus. Our results showed that the weights of fetuses derived from biopsied embryos were lower than that of non-biopsied counterparts at E12.5, E15.5, and E18.5. The ratio of fetal/placental (F/P) weights in the biopsied group was significantly lower than that in the non-biopsied group at E18.5. At E18.5, the mRNAs for selected glucose transporters, system A amino acid transporters, system L amino acid transporters, and imprinted genes were downregulated in the placentae of biopsied group, and the GLUT1 and CAT3 protein levels were decreased too. More apoptotic cells were detected by TUNEL in the placentae of biopsied group. Placentae from biopsied embryos exhibited lower levels of SOD and GSH. Furthermore, the concentration of MDA increased in the placentae from biopsied group. The levels of IL1B, IL6, and TNFA also significantly increased in the placentae of biopsied group. This study suggested that placental function may be sensitive to blastomere biopsy procedures, and placental oxidative stress and inflammation associated with blastomere biopsy may be critical factors of abnormal placental function and further influence the fetal development.
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Qi
Q
Yao
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Li
L
Chen
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Yuanjiao
Y
Liang
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Liucai
L
Sui
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Li
L
Guo
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Jingwei
J
Zhou
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Kai
K
Fan
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Jun
J
Jing
Anhui Provincial Laboratory for Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, PR China.
Yunhai
Y
Zhang
Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, PR China.
Bing
B
Yao
D000818:Animals; D017209:Apoptosis; D001706:Biopsy / Q000009:adverse effects*; D001757:Blastomeres; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D047109:Fetal Development; D020567:Fetal Weight; D007249:Inflammation / Q000209:etiology; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D010920:Placenta / Q000201:enzymology / Q000378:metabolism*; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000009:adverse effects; D015398:Signal Transduction