Uptake, tissue distribution and depuration of triclosan in the guppy Poecilia vivipara acclimated to freshwater.
The agent triclosan has been extensively used in different personal care products as a broad-spectrum antimicrobial and preservative agent. Due to its continuous release into the environment, including discharge via wastewater treatment plants, triclosan has been widely detected in aquatic environments. There is growing interest in improving the knowledge about the environmental fate of triclosan due to its possible bioaccumulation and the toxicity it may pose to organisms, such as fish and other non-target species. To investigate the distribution and bioconcentration of triclosan in fish, Poecilia vivipara was exposed to 0.2mgL(-1). Contents of triclosan in whole fish, brain, gonads, liver, muscle and gills were quantified by LC-MS/MS. When lipid normalised concentration was used, the liver exhibited the highest concentration followed by the gills, gonads, brain and muscle tissues. Bioconcentration was increased with time reaching a steady-state around 7-14days for most all tissues. After 24h depuration, triclosan concentrations declined >80% in all tissues except liver, in which triclosan takes longer to be depurated. These results not only clearly indicate that triclosan accumulated in P. vivipara, with tissue-specific bioconcentration factors (BCF) that ranged from 40.2 to 1025.4, but also show that the elimination of triclosan after transferring the fish to triclosan-free freshwater is rapid in all tissues.
Instituto de Ciências Biológicas, Programa de Pós-graduação em Fisiologia Animal Comparada, Universidade Federal do Rio Grande, Av Itália, km8, s/n, Rio Grande, Rio Grande do Sul State 96203-900, Brazil.
Ana Laura Venquiaruti
AL
Escarrone
Laboratório de Análises de Compostos Orgânicos e Metais, Escola de Química e Alimentos, Programa de Pós-graduação em Química Tecnológica e Ambiental, Universidade Federal do Rio Grande, Av Itália, km8, s/n, Rio Grande, Rio Grande do Sul State 96203-900, Brazil.
Sergiane Souza
SS
Caldas
Laboratório de Análises de Compostos Orgânicos e Metais, Escola de Química e Alimentos, Programa de Pós-graduação em Química Tecnológica e Ambiental, Universidade Federal do Rio Grande, Av Itália, km8, s/n, Rio Grande, Rio Grande do Sul State 96203-900, Brazil.
Ednei Gilberto
EG
Primel
Instituto de Ciências Biológicas, Programa de Pós-graduação em Fisiologia Animal Comparada, Universidade Federal do Rio Grande, Av Itália, km8, s/n, Rio Grande, Rio Grande do Sul State 96203-900, Brazil.
Samantha Eslava
SE
Martins
Instituto de Ciências Biológicas, Programa de Pós-graduação em Fisiologia Animal Comparada, Universidade Federal do Rio Grande, Av Itália, km8, s/n, Rio Grande, Rio Grande do Sul State 96203-900, Brazil. Electronic address: famnery@terra.com.
Luiz Eduardo Maia
LE
Nery
D000818:Animals; D006066:Gonads; D008099:Liver; D011036:Poecilia / Q000378:metabolism*; D014018:Tissue Distribution; D014260:Triclosan / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D014874:Water Pollutants, Chemical; D014260:Triclosan
Bioaccumulation; Bioconcentration factor; Fish; PPCP; Toxicokinetics