In Vitro Propagation and Conservation of Withania somnifera (Dunal) L.
Plant tissue culture offers several techniques for rapid clonal propagation, germplasm conservation, regeneration of genetically manipulated superior clones, production of phyto-constituents, and ex vitro conservation of valuable phytodiversity. An improved and efficient micropropagation protocol for Withania somnifera (L.), a drug-producing medicinal plant, using juvenile explants (nodal explants) has been developed. Highest multiplication and subsequent elongation of shoots is observed on MS medium containing BA and NAA. The regenerated microshoots roots best on ½ MS medium containing NAA, established in earthen pots containing garden soil and are maintained in the greenhouse with 95 % survival rate. Genetic uniformity of micropropagated plants is confirmed by PCR-based DNA fingerprinting techniques, viz., RAPD and ISSR. No variation is observed in DNA fingerprinting patterns among the micropropagated plants, which are similar to that of the donor plant illustrating their genetic uniformity.
Department of Agriculural Microbiology, Aligarh Muslim University, Aligarh (UP), India.
Nigar
N
Fatima
Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, UP, India.|Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Naseem
N
Ahmad
Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, UP, India. anismohd37@gmail.com.|Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia. anismohd37@gmail.com.
Mohammad
M
Anis
D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media / Q000378:metabolism; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D000069600:Plant Breeding / Q000379:methods; D010937:Plant Growth Regulators / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D032341:Withania / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*
D003470:Culture Media; D018744:DNA, Plant; D010937:Plant Growth Regulators
Aseptic seedlings; Clonal fidelity; Clones; Conservation; DNA fingerprinting; Genetic diversity; Genetic stability; Germplasm conservation; Juvenile explants; Micropropagation; PCR amplifications; Phytodiversity; Phytohormones; Soilrite; Stratification