Potential involvement of IQGAP1 in proliferation and metastasis of human pancreatic cancer.
IQGAP1, as a scaffold protein, integrates diverse cellular functions, including rearrangement of the actin cytoskeleton, cell adhesion, gene transcription and cell cycle regulation. IQGAP1 is elevated in a number of human cancer cells. However little is known about the expression of IQGAP in human pancreatic cancer and its association with cancer proliferation or metastasis. In the present study, we examined the expression of IQGAP1 in different pancreatic cancer cell lines and we found that IQGAP1 level is highly correlated with the degree of malignancy of pancreatic cancer cell metastasis. The proliferation, metastasis, motility and tumorigenesis in SW1990 human pancreatic cells were greatly impaired by down-regulating IQGAP1 expression with RNA interference. Mechanistic analysis indicated that Cdc42/Rac1 pathway might contribute to IQGAP1-mediated-pancreatic cell proliferation and tumorigenesis.
Pathology Center, Shanghai First People's Hospital/Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Number 100, Hai-Ning Road, 200080, Shanghai, China.
Yubiao
Y
Jin
Pathology Center, Shanghai First People's Hospital/Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Number 100, Hai-Ning Road, 200080, Shanghai, China.
Xiuhong
X
Lv
Department of Emergency, Yangpu Hospital, Tongji University School of Medicine, Shanghai, 200090, China.
Jilan
J
Zhou
Pathology Center, Shanghai First People's Hospital/Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Number 100, Hai-Ning Road, 200080, Shanghai, China, jiaweichen2000@yeah.net.
Jiawei
J
Chen
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D009362:Neoplasm Metastasis / Q000503:physiopathology; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D034741:RNA, Small Interfering / Q000235:genetics; D020830:rac1 GTP-Binding Protein / Q000378:metabolism; D020703:ras GTPase-Activating Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
C101203:IQ motif containing GTPase activating protein 1; C107953:RAC1 protein, human; D034741:RNA, Small Interfering; D020703:ras GTPase-Activating Proteins; D020830:rac1 GTP-Binding Protein