Novel Strategy for Preparing Dual-Modality Optical/PET Imaging Probes via Photo-Click Chemistry.
Preparation of small molecule based dual-modality probes remains a challenging task due to the complicated synthetic procedure. In this study, a novel concise and generic strategy for preparing dual-modality optical/PET imaging probes via photo-click chemistry was developed, in which the diazole photo-click linker functioned not only as a bridge between the targeting-ligand and the PET imaging moiety, but also as the fluorophore for optical imaging. A dual-modality AE105 peptidic probe was successfully generated via this strategy and subsequently applied in the fluorescent staining of U87MG cells and the (68)Ga based PET imaging of mice bearing U87MG xenograft. In addition, dual-modality monoclonal antibody cetuximab has also been generated via this strategy and labeled with (64)Cu for PET imaging studies, broadening the application of this strategy to include the preparation of macromolecule based imaging probes.
Department of Radiology, University of Pittsburgh , Pittsburgh, Pennsylvania 15219, United States.
Lingyi
L
Sun
Department of Radiology, University of Pittsburgh , Pittsburgh, Pennsylvania 15219, United States.|Department of Radiology, Third Affiliated Hospital of Suzhou University , Changzhou City, Jiangsu 213003, China.
Jiule
J
Ding
Department of Radiology, Third Affiliated Hospital of Suzhou University , Changzhou City, Jiangsu 213003, China.
Wei
W
Xing
Department of Radiology, University of Pittsburgh , Pittsburgh, Pennsylvania 15219, United States.
Yongkang
Y
Gai
Department of Radiology, Third Affiliated Hospital of Suzhou University , Changzhou City, Jiangsu 213003, China.|Department of Radiology, Changhai Hospital of Shanghai , Shanghai, 200433, China.
Jing
J
Sheng
Department of Radiology, University of Pittsburgh , Pittsburgh, Pennsylvania 15219, United States.
Dexing
D
Zeng
D000818:Animals; D001393:Azoles / Q000737:chemistry; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D000068818:Cetuximab / Q000737:chemistry; D057930:Click Chemistry; D006571:Heterocyclic Compounds / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring; D006801:Humans; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D055668:Photochemical Processes; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000737:chemistry*
D001393:Azoles; D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; D019275:Radiopharmaceuticals; C048993:1,4,7-triazacyclononane-N,N',N''-triacetic acid; D000068818:Cetuximab