UPLC-MS/MS assay for simultaneous determination of four compounds in rat plasma: application to pharmacokinetic study after oral administration of Caulis Spatholobi extract.
An ultra-performance liquid chromatography tandem mass spectrometry method was developed for the simultaneous determination of protocatechuic acid, catechin, gallocatechin and formononetin in rat plasma, with genkwanin as the internal standard in this study. Plasma samples were prepared by liquid-liquid extraction with ethyl acetate. The four components were separated on an Agilent Zorbax Eclipse Plus C18column (2.1 × 50 mm, 1.8 μm) with the mobile phase consisting of water containing 0.05% formic acid and methanol (35:65, v/v), and detected by negative ion electrospray ionization in the selected reaction monitoring mode. The method was linear for all analytes over the investigated ranges, with all correlation coefficients >0.99. The validated lower limit of quantification was 0.5 ng/mL for protocatechuic acid, catechin, and gallocatechin and 0.8 ng/mL for formononetin. The intra- and inter-day precisions (RSD, %) were <13.1%, and accuracy (RE, %) ranged from -13.8 to 9.9%. The mean absolute extraction recoveries of the analytes and internal standard from rat plasma were all >80.7%. The validated method was successfully applied for the first time to investigate the pharmacokinetics of four chemical ingredients after oral administration of Caulis Spatholobi Extract in rats. Copyright © 2016 John Wiley & Sons, Ltd.
Department of Vascular Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China.|Department of Vascular Surgery, Linyi Peoples' Hospital Affiliated to Shandong University, Linyi, 276000, China.
Yubin
Y
Li
Department of Vascular Surgery, Linyi Peoples' Hospital Affiliated to Shandong University, Linyi, 276000, China.
Tao
T
Song
Department of Vascular Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China. jxdas324@126.com.
Xing
X
Jin
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D007887:Fabaceae / Q000737:chemistry; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004365:Drugs, Chinese Herbal
Caulis Spatholobi; UPLC-MS/MS; pharmacokinetic study; rat plasma