title: The cyclin D1 carboxyl regulatory domain controls the division and differentiation of hematopoietic cells.
abstract: The family of D cyclins has a fundamental role in cell cycle progression, but its members (D1, D2, D3) are believed to have redundant functions. However, there is some evidence that contradicts the notion of mutual redundancy and therefore this concept is still a matter of debate.
  
  
  
  Our data show that the cyclin D1 is indispensable for normal hematopoiesis. Indeed, in the absence of D1, either in genetic deficient mice, or after acute ablation by RNA interference, cyclins D2 and D3 are also not expressed preventing hematopoietic cell division and differentiation at its earliest stage. This role does not depend on the cyclin box, but on the carboxyl regulatory domain of D1 coded by exons 4-5, since hematopoietic differentiation is also blocked by the conditional ablation of this region.
  
  
  
  These results demonstrate that not all functions of individual D cyclins are redundant and highlight a master role of cyclin D1 in hematopoiesis.
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France.|Present addresses: Instituto de Medicina Molecular, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal.
authors.forename: Miguel
authors.initials: M
authors.lastname: Chaves-Ferreira
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Gerald
authors.initials: G
authors.lastname: Krenn
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France.|Institut Pasteur, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Vasseur
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France.|Institut Pasteur, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Barinov
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Pedro
authors.initials: P
authors.lastname: Gonçalves
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Orly
authors.initials: O
authors.lastname: Azogui
authors.affiliation: Institut Pasteur, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Ana
authors.initials: A
authors.lastname: Cumano
authors.affiliation: Institut Pasteur, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institut Pasteur, 25, Rue du Dr Roux, Cedex 15, Paris, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Pellegrini
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France. benedita.rocha@inserm.fr.|Institut Pasteur, 25, Rue du Dr Roux, Cedex 15, Paris, France. benedita.rocha@inserm.fr.
authors.forename: Benedita
authors.initials: B
authors.lastname: Rocha
authors.affiliation: INSERM, U 1020, U1151 - CNRS, UMR 8253, Institut Necker Enfants Malades, Faculté de Médecine Paris Descartes, 25, Rue du Dr Roux, Cedex 15, Paris, France. diegoladerach@qb.fcen.uba.ar.|IQUIBICEN-CONICET, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes 2160, C1428EGA, Buenos Aires, Argentina. diegoladerach@qb.fcen.uba.ar.
authors.forename: Diego
authors.initials: D
authors.lastname: Laderach
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D056742:Cyclin D2 / Q000235:genetics* / Q000378:metabolism; D056743:Cyclin D3 / Q000235:genetics* / Q000378:metabolism; D006410:Hematopoiesis; D051379:Mice; D008822:Mice, Transgenic
chemical_list: C530105:Ccnd1 protein, mouse; C486196:Ccnd2 protein, mouse; C534697:Ccnd3 protein, mouse; D056742:Cyclin D2; D056743:Cyclin D3; D019938:Cyclin D1
keywords: Cell cycle; D cyclins; Hematopoiesis