title: Plasmodesmata targeting and intercellular trafficking of Tomato spotted wilt tospovirus movement protein NSm is independent of its function in HR induction.
abstract: The movement protein NSm of Tomato spotted wilt tospovirus (TSWV) plays pivotal roles in viral intercellular trafficking. Recently, the TSWV NSm was also identified as an avirulence (Avr) determinant during the Sw-5b-mediated hypersensitive response (HR). However, whether the cell-to-cell movement of NSm is coupled to its function in HR induction remains obscure. Here, we showed that the NSm mutants defective in targeting plasmodesmata and cell-to-cell movement were still capable of inducing Sw-5b-mediated HR. In addition, introduction of a single amino-acid substitution, C118Y or T120N, identified previously from TSWV resistance-breaking isolates, into the movement-defective NSm mutants resulted in the failure of HR induction. Collectively, our results showed that the intercellular trafficking of NSm is uncoupled from its function in HR induction. These findings shed light on the evolutionary mechanism of R-Avr recognition and may be used to explain why this uncoupled phenomenon can be observed in many different viruses.
authors.affiliation: Key Laboratory for the Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Wenyang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Key Laboratory for the Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory for the Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhike
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Key Laboratory for the Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory for the Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory for the Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Xue
authors.affiliation: Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming, 650021, PR China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Huang
authors.affiliation: Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming, 650021, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Plant Protection, Yunnan Agricultural University, Kunming 650201, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, PR China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory for the Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Tao
mesh_terms: D017353:Gene Deletion; D054884:Host-Pathogen Interactions; D018551:Solanum lycopersicum / Q000276:immunology / Q000821:virology*; D020125:Mutation, Missense; D053830:Plant Viral Movement Proteins / Q000187:drug effects / Q000378:metabolism*; D031425:Plasmodesmata / Q000821:virology*; D021381:Protein Transport; D017865:Tospovirus / Q000276:immunology / Q000502:physiology*
chemical_list: D053830:Plant Viral Movement Proteins