title: Graphene/graphene oxide and their derivatives in the separation/isolation and preconcentration of protein species: A review.
abstract: The distinctive/unique electrical, chemical and optical properties make graphene/graphene oxide-based materials popular in the field of analytical chemistry. Its large surface offers excellent capacity to anchor target analyte, making it an powerful sorbent in the adsorption and preconcentration of trace level analyte of interest in the field of sample preparation. The large delocalized π-electron system of graphene framework provides strong affinity to species containing aromatic rings, such as proteins, and the abundant active sites on its surface offers the chance to modulate adsorption tendency towards specific protein via functional modification/decoration. This review provides an overview of the current research on graphene/graphene oxide-based materials as attractive and powerful adsorption media in the separation/isolation and preconcentration of protein species from biological sample matrixes. These practices are aiming at providing protein sample of high purity for further investigations and applications, or to achieve certain extent of enrichment prior to quantitative assay. In addition, the challenges and future perspectives in the related research fields have been discussed.
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hai
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: jianhuajrz@mail.neu.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D053801:Capillary Electrochromatography / Q000379:methods; D011232:Chemical Precipitation; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010087:Oxides / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D010087:Oxides; D011506:Proteins; D006108:Graphite
keywords: Adsorption; Graphene; Graphene oxide; Isolation; Preconcentration; Protein; Separation