title: Remineralizing potential of CPP-ACP applied for 1 min in vitro.
abstract: OBJECTIVE
  To evaluate (1) the remineralizing potential of Tooth Mousse(®) (TM) on artificial carious lesions, when applied for 1 min, and (2) the benefit of a fluoridated dentifrice prior to TM application.
  
  
  METHODS
  Demineralisation caries-like lesions 120-200 μm deep were produced by placing molars in demineralizing solution for 96 h, sections 100-150 μm thick were then randomly assigned to four groups. Specimens were treated thrice daily with a non-fluoridated (Group A), or fluoridated dentifrice (1000 ppm) (Group B), or TM (Group C), or a fluoridated dentifrice followed by TM (Group D) then subjected to a 10-day pH cycling model. Lesion evaluation involved polarizing light microscopy and microradiography.
  
  
  RESULTS
  Application of TM in isolation (Group C) for 1 min resulted in significant increase in lesion depth (LD), however, an increase in mineral content deposition (V max) was not significant. Post treatment LD increases were greatest in Group A, then Group C and Group B respectively. A decrease in LD (by 19.9 %) was noted only after fluoridated dentifrice and TM (Group D) were applied consecutively for 1 min each (p < 0.05). Mineral content deposition was also greatest in this group (Group D) and both V max and LD changes were statistically significant when compared to other groups.
  
  
  CONCLUSIONS
  TM alone failed to remineralize carious lesions when applied for 1 min. However, remineralization occurred after a prior application of a fluoridated dentifrice.
authors.affiliation: Paediatric Dentistry, School of Dentistry, University of Western Australia, 17 Monash Avenue, Nedlands, WA, 6009, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Gopalakrishnan
authors.affiliation: Paediatric Dentistry, School of Dentistry, University of Western Australia, 17 Monash Avenue, Nedlands, WA, 6009, Australia. robert.anthonappa@uwa.edu.au.
authors.forename: R P
authors.initials: RP
authors.lastname: Anthonappa
authors.affiliation: Paediatric Dentistry, School of Dentistry, University of Western Australia, 17 Monash Avenue, Nedlands, WA, 6009, Australia.
authors.forename: N M
authors.initials: NM
authors.lastname: King
authors.affiliation: Paediatric Dentistry, School of Dentistry and Oral Health, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Itthagarun
mesh_terms: D002327:Cariostatic Agents / Q000008:administration & dosage*; D002364:Caseins / Q000008:administration & dosage*; D003731:Dental Caries / Q000473:pathology / Q000628:therapy*; D003743:Dental Enamel / Q000737:chemistry / Q000473:pathology*; D005459:Fluorides / Q000008:administration & dosage; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D011897:Random Allocation; D014089:Tooth Remineralization / Q000379:methods*
chemical_list: D002327:Cariostatic Agents; D002364:Caseins; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D005459:Fluorides
keywords: CPP-ACP; Demineralization; Dental caries; Dentifrice; Fluoride; Remineralization