title: Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination.
abstract: Nitrogen-doped carbon dots are introduced as a novel substrate suitable for enzyme immobilization in electrochemical detection metods. Nitrogen-doped carbon dots are easily synthesised from polyacrylamide in just one step. With the help of the amino group on chitosan, glucose oxidase is immobilized on nitrogen-doped carbon dots-modified carbon glassy electrodes by amino-carboxyl reactions. The nitrogen-induced charge delocalization at nitrogen-doped carbon dots can enhance the electrocatalytic activity toward the reduction of O₂. The specific amino-carboxyl reaction provides strong and stable immobilization of GOx on electrodes. The developed biosensor responds efficiently to the presence of glucose in serum samples over the concentration range from 1 to 12 mM with a detection limit of 0.25 mM. This novel biosensor has good reproducibility and stability, and is highly selective for glucose determination under physiological conditions. These results indicate that N-doped quantum dots represent a novel candidate material for the construction of electrochemical biosensors.
authors.affiliation: State Key Laboratory of Coordination Chemistry, Department of Polymer Science & Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210023, China. com_jihanxv@163.com.
authors.forename: Hanxu
authors.initials: H
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Coordination Chemistry, Department of Polymer Science & Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210023, China. zhoufeng819@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Coordination Chemistry, Department of Polymer Science & Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210023, China. gjjdgqf@gmail.com.
authors.forename: Jiangjiang
authors.initials: J
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Coordination Chemistry, Department of Polymer Science & Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210023, China. gandalf404@126.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Shu
authors.affiliation: Department of Polymer Science & Engineering, Nanjing University, Nanjing 210023, China. xikai@nju.edu.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Xi
authors.affiliation: State Key Laboratory of Coordination Chemistry, Department of Polymer Science & Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210023, China. jiaxd@nju.edu.cn.
authors.forename: Xudong
authors.initials: X
authors.lastname: Jia
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon; D004566:Electrodes; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D037742:Nanotubes, Carbon; D009584:Nitrogen; D015203:Reproducibility of Results
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose; D009584:Nitrogen
keywords: biosensor; electrochemical detection; enzyme immobilization; glucose oxidase; nitrogen-doped carbon dot