title: Effective elimination of liver cancer stem-like cells by CD90 antibody targeted thermosensitive magnetoliposomes.
abstract: OBJECTIVE
  To investigate the use of thermosensitive magnetoliposomes (TMs) loaded with magnetic iron oxide (Fe3O4) and the anti-cancer stem cell marker CD90 (CD90@TMs) to target and kill CD90+ liver cancer stem cells (LCSCs).
  
  
  METHODS
  The hepatocellular carcinoma cell line Huh7 was used to separate CD90+ LCSCs by magnetic-activated cell sorting. CD90@TMs was characterized and their ability to target CD90+ LCSCs was determined. Experiments were used to investigate whether CD90@TMs combined with magnetic hyperthermia could effectively eliminate CD90+ LCSCs.
  
  
  RESULTS
  The present study demonstrated that CD90+ LCSCs with stem cells properties were successfully isolated. We also successfully prepared CD90@TMs that was almost spherical and uniform with an average diameter of 130±4.6 nm and determined that magnetic iron oxide could be incorporated and retained a superparamagnetic response. CD90@TMs showed good targeting and increased inhibition of CD90+ LCSCs in vitro and in vivo compared to TMs.
  
  
  CONCLUSIONS
  CD90@TMs can be used for controlled and targeted delivery of anticancer drugs, which may offer a promising alternative for HCC therapy.
authors.affiliation: School of Medicine, Southeast University, Nanjing, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Jiangsu Key Laboratory of Molecular and Fuctional Imaging, Department of Radiology, Zhongda Hospital, Nanjing, People's Republic of China.
authors.forename: Li Y
authors.initials: LY
authors.lastname: An
authors.affiliation: School of Medicine, Southeast University, Nanjing, People's Republic of China.
authors.forename: Qin F
authors.initials: QF
authors.lastname: Miao
authors.affiliation: School of Medicine, Southeast University, Nanjing, People's Republic of China.
authors.forename: Feng M
authors.initials: FM
authors.lastname: Li
authors.affiliation: School of Medicine, Southeast University, Nanjing, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Medicine, Southeast University, Nanjing, People's Republic of China.
authors.forename: Hui X
authors.initials: HX
authors.lastname: Wang
authors.affiliation: School of Medicine, Southeast University, Nanjing, People's Republic of China.
authors.forename: Dang P
authors.initials: DP
authors.lastname: Liu
authors.affiliation: Department of Oncology, Zhongda Hospital, Nanjing, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: School of Medicine, Southeast University, Nanjing, People's Republic of China.
authors.forename: Sha Q
authors.initials: SQ
authors.lastname: Tang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008113:Liver Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013696:Temperature; D018800:Thy-1 Antigens / Q000276:immunology* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D000906:Antibodies; D018800:Thy-1 Antigens
keywords: CD90; LCSCs; TMs; hyperthermia therapy; targeting therapy