title: Wound dressing based on chitosan/hyaluronan/nonwoven fabrics: Preparation, characterization and medical applications.
abstract: Thin layers of chitosan (positively charged)/sodium hyaluronate (negatively charged)/nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different techniques such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.
authors.affiliation: Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic. Electronic address: rmmar2008@yahoo.com.
authors.forename: Rasha M
authors.initials: RM
authors.lastname: Abdel-Rahman
authors.affiliation: CEITEC-Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; National Research Centre, Textile Research Division, Pre-Treatment and Finishing of Cellulosic Fibers, Dokki, Cairo, Egypt; SCITEG,a.s., Brno, Czech Republic. Electronic address: abdel-mohsen@ceitec.vutbr.cz.
authors.forename: A M
authors.initials: AM
authors.lastname: Abdel-Mohsen
authors.affiliation: Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Hrdina
authors.affiliation: Institute of Chemistry and Technology of Macromolecular Compounds, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Burgert
authors.affiliation: CEITEC-Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Fohlerova
authors.affiliation: Department of Physical Electronics, Masaryk University, Brno, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Pavliňák
authors.affiliation: Chemistry Department, Faculty of Science, Fayoum University, Fayoum, Egypt.
authors.forename: O N
authors.initials: ON
authors.lastname: Sayed
authors.affiliation: CEITEC-Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; National Research Centre, Textile Research Division, Pre-Treatment and Finishing of Cellulosic Fibers, Dokki, Cairo, Egypt; Faculty of Chemistry, Institute of Materials Chemistry, Brno University of Technology, Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Jancar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000627:therapeutic use; D001458:Bandages / Q000382:microbiology*; D048271:Chitosan / Q000737:chemistry / Q000627:therapeutic use; D004926:Escherichia coli / Q000187:drug effects / Q000472:pathogenicity; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use; D051379:Mice; D041681:NIH 3T3 Cells; D013211:Staphylococcus aureus / Q000187:drug effects / Q000472:pathogenicity; D013784:Textiles / Q000382:microbiology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D006820:Hyaluronic Acid; D048271:Chitosan
keywords: Chitosan; Cytotoxicity; Healing properties; Hyaluronan; Nonwoven fabrics