title: [IFN-γ stimulates the release of cathepsin S in mouse mast cells].
abstract: OBJECTIVE
  To investigate the impact of interferon γ (IFN-γ) on cathepsin S (CTSS) expressed by P815 mouse mast cells and mouse bone marrow-derived mast cells (BMMCs).
  
  
  METHODS
  IFN-γ of 10, 25 and 50 ng/mL were respectively used to stimulate P815 cells and mouse BMMCs. Real-time PCR and ELISA were applied to detect mRNA and protein levels of CTSS in P815 cells and BMMCs. 25 ng/mL IFN-γ was used to treat P815 cells for 6, 12, 18, 24, 48 and 72 hours and mouse BMMCs for 12, 24 and 48 hours; the above mentioned detection steps were then repeated.
  
  
  RESULTS
  IFN-γ induced P815 cells and BMMCs to express CTSS on the mRNA and protein levels in the time- and dose-dependent manners.
  
  
  CONCLUSIONS
  IFN-γ could stimulate mast cells to release CTSS.
authors.affiliation: Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Ouyang
authors.affiliation: Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Danbing
authors.initials: D
authors.lastname: Fan
authors.affiliation: Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. *Corresponding author, E-mail: minyanggz@yahoo.com.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma; D002403:Cathepsins; C028217:cathepsin S