title: Design and automated production of 11C-alpha-methyl-l-tryptophan (11C-AMT).
abstract: (11)C-alpha-methyl-l-tryptophan ([(11)C]AMT), a tryptophan metabolism PET tracer, has successfully been employed for brain serotonin pathway and indoleamine 2,3-dioxygenase (IDO) pathway related tumor imaging. We here report a reliable, automated procedure for routine synthesis of [(11)C]AMT based on an Eckert and Ziegler Modular-Lab system. The semi-preparative HPLC was incorporated into the system to improve chemical purity and specific activity. The 6-step radiosynthesis followed by HPLC-purification provided [(11)C]AMT in 5.3±1.2% (n=6, non-decay-corrected) overall radiochemical yield with radiochemical purity >99% and specific activity of 35-116GBq/μmol. Usually, 2.95±0.65GBq (n=6, EOS) patient ready dose was produced from about 55.5GBq [(11)C]CO2 in 50min.
authors.affiliation: Dept of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Dept of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA; Dept of Pathology, Guangzhou University of Chinese Medicine, Guang Zhou, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Dept of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Gillies
authors.affiliation: Dept of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA. Electronic address: haibin.tian@moffitt.org.
authors.forename: Haibin
authors.initials: H
authors.lastname: Tian
mesh_terms: D001331:Automation; D015195:Drug Design; D049268:Positron-Emission Tomography; D011786:Quality Control; D011854:Radiochemistry / Q000295:instrumentation / Q000379:methods*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: C020774:alpha-methyltryptophan; D014364:Tryptophan