title: Molecular and cytogenetic assessment of Dipterygium glaucum genotoxicity.
abstract: The aim of the present study is to assess the genotoxicity of Dipterygium glaucum grows widely in Saudi Arabia desert to produce safety herbal products. This work is considered the first and pioneer report so far due to the lack and poor evaluated reports of the plant species for their mutagensity, genotoxicity and cytogenetics effects. Cytogenetic effects of D. glaucum on mitotic in roots of Vicia faba showed reduction in mitotic activity using three extracts; water, ethanol and ethyl acetate. Chromosomal abnormalities were recorded that included stickiness of chromosomes, chromatin bridge, fragments, lagging chromosome and micronuclei. Protein bands and RAPD analyses of V. faba treated with three D. glaucum extracts revealed some newly induced proteins and DNA fragments and other disappeared. Chemical constitution of the plant species should be identified with their biological activities against human and animal cells like HeLa cancer cell line. We are recommending using additional genotoxicity tests and other toxicity tests on animal culture with different concentrations and also utilizing several drought and heat tolerant genes of the plant species in gene cloning to develop and improve other economical crop plants instead of using the species as oral herbal remedy.
authors.affiliation: Department of Biology, Faculty of Scienc, King Abdulaziz University, P.O. Box 80200, Zip Code 21589, Saudi Arabia, King Abdulaziz University, Department of Biology, Faculty of Scienc, King Abdulaziz University, Saudi Arabia.
authors.forename: Nada H
authors.initials: NH
authors.lastname: Altwaty
authors.affiliation: Department of Biology, Faculty of Scienc, King Abdulaziz University, P.O. Box 80200, Zip Code 21589, Saudi Arabia, King Abdulaziz University, Department of Biology, Faculty of Scienc, King Abdulaziz University, Saudi Arabia.|Genetics and Cytology Dept, National Research Centre, El-Buhouth St., Dokki, Postal Code 12311, Zip Code 12622, Cairo, Egypt, Genetics and Cytology Dept, National Research Centre, Cairo , Egypt.
authors.forename: Osama E
authors.initials: OE
authors.lastname: El-Sayed
authors.affiliation: Department of Biology, Faculty of Scienc, King Abdulaziz University, P.O. Box 80200, Zip Code 21589, Saudi Arabia, King Abdulaziz University, Department of Biology, Faculty of Scienc, King Abdulaziz University, Saudi Arabia.|Genetics and Cytology Dept, National Research Centre, El-Buhouth St., Dokki, Postal Code 12311, Zip Code 12622, Cairo, Egypt, Genetics and Cytology Dept, National Research Centre, Cairo , Egypt.
authors.forename: Nariman A H
authors.initials: NA
authors.lastname: Aly
authors.affiliation: Department of Biology, Faculty of Scienc, King Abdulaziz University, P.O. Box 80200, Zip Code 21589, Saudi Arabia, King Abdulaziz University, Department of Biology, Faculty of Scienc, King Abdulaziz University, Saudi Arabia.
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Baeshen
authors.affiliation: Department of Biology, Faculty of Scienc, King Abdulaziz University, P.O. Box 80200, Zip Code 21589, Saudi Arabia, King Abdulaziz University, Department of Biology, Faculty of Scienc, King Abdulaziz University, Saudi Arabia.
authors.forename: Nabih A
authors.initials: NA
authors.lastname: Baeshen
mesh_terms: D000818:Animals; D031483:Capparaceae / Q000633:toxicity*; D004249:DNA Damage; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000633:toxicity*; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics; D010947:Plants, Toxic / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique; D031307:Vicia faba / Q000187:drug effects* / Q000235:genetics
chemical_list: D010936:Plant Extracts