title: [Activity of liver mitochondrial NAD+-dependent dehydrogenases of the krebs cycle in rats with acetaminophen-induced hepatitis developed under conditions of alimentary protein deficiency].
abstract: Activity of isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, malate dehydrogenase, and the NAD(+)/NADН ratio were studied in the liver mitochondrial fraction of rats with toxic hepatitis induced by acetaminophen under conditions of alimentary protein deprivation. Acetaminophen-induced hepatitis was characterized by a decrease of isocitrate dehydrogenase, α-ketoglutarate dehydrogenase and malate dehydrogenase activities, while the mitochondrial NAD(+)/NADН ratio remained at the control level. Modeling of acetaminophen-induced hepatitis in rats with alimentary protein caused a more pronounced decrease in the activity of NAD(+)-dependent dehydrogenases studied and a 2.2-fold increase of the mitochondrial NAD(+)/NADН ratio. This suggests that alimentary protein deprivation potentiated drug-induced liver damage.
authors.affiliation: Fedkovych Chernovtsi National University, Chernovtsi, Ukraine.
authors.forename: O N
authors.initials: ON
authors.lastname: Voloshchuk
authors.affiliation: Fedkovych Chernovtsi National University, Chernovtsi, Ukraine.
authors.forename: G P
authors.initials: GP
authors.lastname: Kopylchuk
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000187:drug effects; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D011488:Protein Deficiency / Q000201:enzymology* / Q000209:etiology; D051381:Rats
chemical_list: D009243:NAD; D000082:Acetaminophen; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex
keywords: a-ketoglutarate dehydrogenase; acetaminophen-induced hepatitis; alimentary protein deprivation; isocitrate dehydrogenase; malate dehydrogenase