title: System biology analysis of long-term effect and mechanism of Bufei Yishen on COPD revealed by system pharmacology and 3-omics profiling.
abstract: System pharmacology identified 195 potential targets of Bufei Yishen formula (BYF), and BYF was proven to have a short-term therapeutic effect on chronic obstructive pulmonary disease (COPD) rats previously. However, the long-term effect and mechanism of BYF on COPD is still unclear. Herein, we explored its long-term effect and underlying mechanism at system level. We administered BYF to COPD rats from week 9 to 20, and found that BYF could prevent COPD by inhibiting the inflammatory cytokines expression, protease-antiprotease imbalance and collagen deposition on week 32. Then, using transcriptomics, proteomics and metabolomics analysis, we identified significant regulated genes, proteins and metabolites in lung tissues of COPD and BYF-treated rats, which could be mainly attributed to oxidoreductase-antioxidant activity, focal adhesion, tight junction or lipid metabolism. Finally, based on the comprehensive analysis of system pharmacology target, transcript, protein and metabolite data sets, we found a number of genes, proteins, metabolites regulated in BYF-treated rats and the target proteins of BYF were involved in lipid metabolism, inflammatory response, oxidative stress and focal adhension. In conclusion, BYF exerts long-term therapeutic action on COPD probably through modulating the lipid metabolism, oxidative stress, cell junction and inflammatory response pathways at system level.
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Yang
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Yange
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.|Department of Respiratory Diseases, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China.
authors.forename: Suyun
authors.initials: S
authors.lastname: Li
mesh_terms: D000628:Aminophylline / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001993:Bronchodilator Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D022001:Focal Adhesions / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D055432:Metabolomics; D018384:Oxidative Stress / Q000187:drug effects; D010366:Peak Expiratory Flow Rate / Q000187:drug effects; D008517:Phytotherapy / Q000379:methods*; D040901:Proteomics; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D049490:Systems Biology; D013990:Tidal Volume / Q000187:drug effects; D019108:Tight Junctions / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D001993:Bronchodilator Agents; C574914:Bu-Fei-Yi-Shen; D004365:Drugs, Chinese Herbal; D000628:Aminophylline