title: Overexpression of micro ribonucleic acid-591 inhibits cell proliferation and invasion of malignant pleural mesothelioma cells.
abstract: Malignant pleural mesothelioma (MPM) is an aggressive cancer refractory to current therapies. Reduced expression of micro ribonucleic acid (miR)-591 in a range of cancer types has suggested it is a potent tumor suppressor, and overexpression has been shown to inhibit tumor cell growth. The role of miR-591 in MPM is largely unknown.
  
  
  
  miR-591 was over-expressed in vitro using micro RNA mimics in three MPM cell lines (H513, H2052, H2373), and effects on tumor cell growth, proliferation, invasion, and target gene expression were assessed.
  
  
  
  miR-591 mimic was introduced into MPM cell lines to overexpress this microRNA. The cellular growth, proliferation, and invasive capability was significantly inhibited after overexpression of miR-591. Growth inhibition caused by miR-591 correlated with upregulation of p21 and Bax. Reduced invasive capability correlated with downregulation of matrix metalloproteinase-2 and transforming growth factor-β1.
  
  
  
  miR-591 is a potent tumor suppressor in MPM. Overexpression of miR-591 may represent a novel therapeutic approach for MPM.
authors.affiliation: Tianjin Medical University Tianjin China; Department of Thoracic Surgery Tianjin Chest Hospital Tianjin China.
authors.forename: Shizhao
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Division of Thoracic Surgery Department of Cardiothoracic Surgery Stanford University School of Medicine Stanford California USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Thoracic Surgery Tianjin Chest Hospital Tianjin China.
authors.forename: Zhenliang
authors.initials: Z
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Oncology in Southern China Department of Thoracic Surgery Sun Yat-Sen University Cancer Center Guangzhou China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Division of Thoracic Surgery Department of Cardiothoracic Surgery Stanford University School of Medicine Stanford California USA.
authors.forename: Chuong D
authors.initials: CD
authors.lastname: Hoang
authors.affiliation: Department of Thoracic Surgery Tianjin Chest Hospital Tianjin China.
authors.forename: Xun
authors.initials: X
authors.lastname: Zhang
keywords: Malignant pleural mesothelioma; miR‐591; microRNA