title: [Antiapoptotic Effect of the Leukemia Associated Gene MLAA-34 in HeLa Cells].
abstract: OBJECTIVE
  To study the antiapoptotic effect of leukemia-associated gene MLAA-34 in HeLa cells.
  
  
  METHODS
  The MLAA-34 recombinant lentiviral expression vector was constructed, and the stably transfected HeLa cell line with high expression of MLAA-34 was set up; As(2)O(3) was used to induce apoptosis; the MTT assay, colony formation test and flow cytometry were used to detect the ability of cell proliferation, colong formation, apoptosis and cell cycle changes respectively.
  
  
  RESULTS
  After treatment with As(2)O(3), the survival rate of HeLa cells with MLAA-34 overexpression was significantly higher than that of the control cells, and the colony formation ability of MLAA-34 significantly increased, and the high expression of MLAA-34 gene significantly decreased the apoptosis rate of HeLa cells, and decreased the proportion of G(2)/M phase cells.
  
  
  CONCLUSIONS
  The leukemia-associated gene MLAA-34 has been comfirmed to show antiapoptotic effect in HeLa cells which are induced by As(2)O(3).
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China. E-mail: zhangpengyu2004@126.com.
authors.forename: Peng-Yu
authors.initials: PY
authors.lastname: Zhang
authors.affiliation: Department of Histology & Embryology, Xi'an Medical College, Xi'an 710021, Shaanxi Province, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Shaanxi Blood Center, Xi'an 710061, Shaanxi Province, China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Wang-Gang
authors.initials: WG
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Yin-Xia
authors.initials: YX
authors.lastname: Chen
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D000077237:Arsenic Trioxide; D001152:Arsenicals; D002453:Cell Cycle; D049109:Cell Proliferation; D006367:HeLa Cells; D006801:Humans; D016086:Lentivirus; D010087:Oxides; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D051017:Apoptosis Regulatory Proteins; D001152:Arsenicals; C534238:CAB39L protein, human; D010087:Oxides; D000077237:Arsenic Trioxide