title: Pias1 is essential for erythroid and vascular development in the mouse embryo.
abstract: The protein inhibitor of activated STAT-1 (PIAS1) is one of the few known SUMO E3 ligases. PIAS1 has been implicated in several biological processes including repression of innate immunity and DNA repair. However, PIAS1 function during development and tissue differentiation has not been studied. Here, we report that Pias1 is required for proper embryonic development. Approximately 90% of Pias1 null embryos die in utero between E10.5 and E12.5. We found significant apoptosis within the yolk sac (YS) blood vessels and concomitant loss of red blood cells (RBCs) resulting in profound anemia. In addition, Pias1 loss impairs YS angiogenesis and results in defective capillary plexus formation and blood vessel occlusions. Moreover, heart development is impaired as a result of loss of myocardium muscle mass. Accordingly, we found that Pias1 expression in primary myoblasts enhances the induction of cardiac muscle genes MyoD, Myogenin and Myomaker. PIAS1 protein regulation of cardiac gene transcription is dependent on transcription factors Myocardin and Gata-4. Finally, endothelial cell specific inactivation of Pias1 in vivo impairs YS erythrogenesis, angiogenesis and recapitulates loss of myocardium muscle mass. However, these defects are not sufficient to recapitulate the lethal phenotype of Pias1 null embryos. These findings highlight Pias1 as an essential gene for YS erythropoiesis and vasculogenesis in vivo.
authors.affiliation: Department of Internal Medicine and Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Jerfiz D
authors.initials: JD
authors.lastname: Constanzo
authors.affiliation: Departments of Physiology, and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Mi
authors.initials: M
authors.lastname: Deng
authors.affiliation: Department of Internal Medicine and Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Smita
authors.initials: S
authors.lastname: Rindhe
authors.affiliation: Department of Internal Medicine and Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Pulmonary Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Tang
authors.affiliation: Departments of Physiology, and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine and Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: Pier.Scaglioni@UTSouthwestern.edu.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Scaglioni
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology; D004920:Erythropoiesis / Q000235:genetics / Q000502:physiology*; D005317:Fetal Growth Retardation / Q000235:genetics / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D005804:Genes, Lethal; D005855:Germ Layers / Q000166:cytology; D006321:Heart / Q000196:embryology; D008264:Macrophages / Q000166:cytology; D051379:Mice; D038042:Myelopoiesis / Q000235:genetics / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000235:genetics / Q000502:physiology*; D019683:Penetrance; D050828:Protein Inhibitors of Activated STAT / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D058207:Sumoylation; D014157:Transcription Factors / Q000502:physiology; D015017:Yolk Sac / Q000098:blood supply / Q000254:growth & development
chemical_list: C494211:Pias1 protein, mouse; D050828:Protein Inhibitors of Activated STAT; D014157:Transcription Factors
keywords: Angiogenesis; Embryonic development; Fetal erythropoiesis; Pias1; Yolk sac capillary plexus