title: Epithelial expression of cytokeratins 15 and 19 in vitiligo.
abstract: BACKGROUND
  Cytokeratins (CK) belong to the family of intermediate filament proteins, and among them specific epithelial keratins are considered markers for stem cells activation.
  
  
  OBJECTIVE
  This study aims to investigate the expression of CK15 and CK19 as possible stem cell markers in vitiligo during phototherapy.
  
  
  METHODS
  The study was conducted on vitiligo patients receiving narrow-band ultraviolet therapy. Immunohistochemical staining for CK15 and CK19 was carried out, and clinical follow-up continued for 4 weeks.
  
  
  RESULTS
  Of 28 patients, CK15 expression was demonstrated in 17 cases (61%) while CK19 expression was demonstrated in 11 cases (39%). Cells expressing positive staining were demonstrated in follicular and interfollicular epithelium. Expression was clearly demonstrated in patients younger than 20 years old, with shorter disease duration, with disease stability, and with normally pigmented hairs. Expression of cytokeratins was significantly correlated to improvement of vitiligo lesions.
  
  
  CONCLUSIONS
  CK15 and CK19 are expressed in vitiligo during UV repigmentation in the follicular and interfollicular epithelium. This expression of cytokeratins was significantly correlated to improvement and can be considered valuable tool to monitor stem cells stimulation for the sake of the repigmentation process in vitiligo.
authors.affiliation: Department of Dermatology, STDs and Andrology, Faculty of Medicine, Minia University, Minia, Egypt.
authors.forename: Fatma Y
authors.initials: FY
authors.lastname: Saleh
authors.affiliation: Department of Dermatology, STDs and Andrology, Faculty of Medicine, Minia University, Minia, Egypt.
authors.forename: Sherif S
authors.initials: SS
authors.lastname: Awad
authors.affiliation: Department of Dermatology, STDs and Andrology, Faculty of Medicine, Minia University, Minia, Egypt.
authors.forename: Ghada A
authors.initials: GA
authors.lastname: Nasif
authors.affiliation: Department of Dermatology, STDs and Andrology, Faculty of Medicine, Minia University, Minia, Egypt.
authors.forename: Christein
authors.initials: C
authors.lastname: Halim
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D002648:Child; D004848:Epithelium / Q000737:chemistry*; D005260:Female; D006801:Humans; D053569:Keratin-15 / Q000032:analysis*; D053539:Keratin-19 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000737:chemistry; D013997:Time Factors; D014467:Ultraviolet Therapy; D014820:Vitiligo / Q000378:metabolism* / Q000532:radiotherapy*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D053569:Keratin-15; D053539:Keratin-19
keywords: cytokeratins; phototherapy; vitiligo