title: Non-Uniform-Sampling Ultrahigh Resolution TOCSY NMR: Analysis of Complex Mixtures at Microgram Levels.
abstract: Non-uniform sampling in combination with homonuclear broadband decoupling along an indirect dimension, and indirect covariance processing are used to record ultrahigh resolution two-dimensional TOCSY spectra in less than half an hour, for typical sample concentrations in the mm range. TOCSY correlations belonging to protons separated by as little as ≈2 Hz can be distinctly discerned. The utility of the technique for low concentrations has been demonstrated.
authors.affiliation: UM-DAE Centre for Excellence in Basic Sciences, Mumbai University Campus, Kalina, Santa Cruz, Mumbai, 400 098, India.
authors.forename: Veera M R
authors.initials: VM
authors.lastname: Kakita
authors.affiliation: UM-DAE Centre for Excellence in Basic Sciences, Mumbai University Campus, Kalina, Santa Cruz, Mumbai, 400 098, India. hosur@tifr.res.in.|Department of Chemical Sciences, Tata Institute of Fundamental Research (TIFR), 1-Homi Bhabha Road, Colaba, Mumbai, 400 005, India. hosur@tifr.res.in.
authors.forename: Ramakrishna V
authors.initials: RV
authors.lastname: Hosur
mesh_terms: D045424:Complex Mixtures / Q000737:chemistry*; D004958:Estradiol / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D013739:Testosterone / Q000032:analysis
chemical_list: D045424:Complex Mixtures; D011522:Protons; D013739:Testosterone; D004958:Estradiol
keywords: fast screening; homodecoupling; indirect covariance processing; micromolar concentrations; non-uniform sampling