title: Cloning and localization of immediate early response 2 (ier2) gene in the brain of medaka.
abstract: Immediate early response (IER) 2 gene, a member of the IER family, is a gene of unknown function which is affected by external stimuli in the brain. In the present study, the full length sequence and localization of medaka (Oryzias latipes) ier2 was investigated in the brain to understand the functions of Ier2 in the future studies. The full length sequence of medaka ier2 was identified using a 3'-, 5'- rapid amplification of cDNA ends method, and distribution in the brain was identified using in situ hybridization. The identified full length ier2 mRNA consisted of 939 nucleotides spanning along 1 exon. The deduced amino acid sequence consisted of 171 amino acid residues which contains a highly conserved sequence, nuclear localization signal. ier2 mRNA was distributed in the telencephalon, midbrain and the hypothalamus. This highly conserved primary response gene Ier2 can be used to visualize and map functionally activated neuronal circuitry in the brain of medaka.
authors.affiliation: Brain Research Institutes, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor 47500, Malaysia. Electronic address: moriya.shogo@monash.edu.
authors.forename: Shogo
authors.initials: S
authors.lastname: Moriya
authors.affiliation: Brain Research Institutes, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor 47500, Malaysia.
authors.forename: Dipti
authors.initials: D
authors.lastname: Chourasia
authors.affiliation: Brain Research Institutes, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor 47500, Malaysia.
authors.forename: Kai We
authors.initials: KW
authors.lastname: Ng
authors.affiliation: Brain Research Institutes, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor 47500, Malaysia.
authors.forename: Nazmina Bahadur
authors.initials: NB
authors.lastname: Khel
authors.affiliation: Brain Research Institutes, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor 47500, Malaysia.
authors.forename: Ishwar S
authors.initials: IS
authors.lastname: Parhar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D029941:Fish Proteins / Q000096:biosynthesis / Q000235:genetics*; D007031:Hypothalamus / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008636:Mesencephalon / Q000378:metabolism; D009990:Oryzias / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013687:Telencephalon / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D017874:Immediate-Early Proteins; D012333:RNA, Messenger
keywords: Full length sequence; Ier2; In situ hybridization; Medaka