title: A novel method for primary neuronal culture and characterization under different high temperature.
abstract: Heatstroke is a big threat to human health; however, the characteristic of pathological changes of neurons during heatstroke development remains unclear. Here, using an in vitro model of primary cultured neurons from newborn Wistar rats, we investigated the effects of the different combinations of high temperature (37, 39, 41, 43, 45, and 47°C) and exposure time (45 min and 1 h) on the neurons. We found that, under the treatment of 45 min-heat, the neurons could resist high temperature up to 45°C, and under the treatment of 1 h-heat, the mortality of neurons increased as the temperature rises. After heating for 1 h, only a small minority of the neurons died under 41 and 43°C, which primarily occurred in the form of apoptosis. Up to 45°C for 1 h, most neurons occurred to necrosis. Meaningfully, some necrotic neurons expressed specific fried egg-like morphology. Our findings suggest that different high temperatures and exposure times were two key factors influencing the death of neurons. Under the high temperature (below 43°C for 1 h) similar to heatstroke, it just led a small percentage of neurons to apoptosis, and anti-apoptosis controls for preventing and treating heatstroke are promising.
authors.affiliation: Department of Neurology, Graduate School of the Second Military Medical University, Shanghai, China.|Department of Neurology, General Hospital of Jinan Military Command of PLA, 25 Shifan Road, Jinan, 250031, China.|Department of Neurology, The 303th Hospital of People's Liberation Army, Nanning, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Neurology, General Hospital of Jinan Military Command of PLA, 25 Shifan Road, Jinan, 250031, China.
authors.forename: Huaiqiang
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Neurology, General Hospital of Jinan Military Command of PLA, 25 Shifan Road, Jinan, 250031, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tao
authors.affiliation: Department of Neurology, General Hospital of Jinan Military Command of PLA, 25 Shifan Road, Jinan, 250031, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Niu
authors.affiliation: Department of Neurology, Bethune International Peace Hospital of the Chinese PLA, Shijiazhuang, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Jiao
authors.affiliation: Department of Burn and Plastic Surgery, The 205th Hospital of People's Liberation Army, Jinzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The National Hospital of Guangxi Province, Nanning, China.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, General Hospital of Jinan Military Command of PLA, 25 Shifan Road, Jinan, 250031, China. m13217809477@163.com.
authors.forename: Bingzhen
authors.initials: B
authors.lastname: Cao
mesh_terms: D000818:Animals; D016923:Cell Death; D018883:Heat Stroke / Q000209:etiology / Q000473:pathology*; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D009474:Neurons / Q000473:pathology / Q000502:physiology*; D061251:Primary Cell Culture; D051381:Rats
keywords: Cortex; Heatstroke; Neurons; Primary culture