title: In Vivo Analysis of Intestinal Mononuclear Phagocytes.
abstract: The study of the intestinal dendritic cell (DC) compartment, its homeostasis, regulation, and response to challenges calls for the investigation within the physiological tissue context comprising the unique anatomic constellation of the epithelial single cell layer and the luminal microbiota, as well as neighboring immune and nonimmune cells. Here we provide protocols we developed that use a combination of conditional cell ablation, conditional compartment mutagenesis, and adoptive precursor transfers to study DC and other intestinal mononuclear phagocytes in in vivo context. We will highlight pitfalls and strengths of these approaches.
authors.affiliation: Department of Immunology, The Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: Caterina
authors.initials: C
authors.lastname: Curato
authors.affiliation: Department of Immunology, The Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: Biana
authors.initials: B
authors.lastname: Bernshtein
authors.affiliation: Department of Immunology, The Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: Tegest
authors.initials: T
authors.lastname: Aychek
authors.affiliation: Department of Immunology, The Weizmann Institute of Science, Rehovot, 76100, Israel. s.jung@weizmann.ac.il.
authors.forename: Steffen
authors.initials: S
authors.lastname: Jung
mesh_terms: D000818:Animals; D000073883:CX3C Chemokine Receptor 1 / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D004167:Diphtheria Toxin / Q000494:pharmacology; D066257:Heparin-binding EGF-like Growth Factor / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D016296:Mutagenesis
chemical_list: D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D004167:Diphtheria Toxin; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor
keywords: Cell ablation; Conditional mutagenesis; Dendritic cells; Intestine; Mononuclear phagocytes