title: [Protein Identification of Blood Nucleoprotein Complexes].
abstract: Circulating nucleoprotein complexes were isolated-from blood plasma by affinity chromatography using immobilized polyclonal anti-histone antibodies. It was found, that the main part of DNA from histone-contained nucleoprotein complexes have size 170-180 b.p., in blood of breast cancer patients DNA with size 170-180 b.p. and DNA more then 6 k.b.p. are presented in equal quantity. Proteins from circulating nucleoprotein complexes were identified using MALDI-TOF mass-spectrometry. It was shown that nucleoprotein complexes from blood of breast cancer patients contain tumor-specific proteins, such as LDOC1L, ADP/ATP translocase 3 and Lamellipodin. These data indicate, that a part of circulating extracellular DNA have tumor origin.
authors.forename: S N
authors.initials: SN
authors.lastname: Tamkovich
authors.forename: D S
authors.initials: DS
authors.lastname: Serdukov
authors.forename: O S
authors.initials: OS
authors.lastname: Tutanov
authors.forename: T G
authors.initials: TG
authors.lastname: Duzhak
authors.forename: P P
authors.initials: PP
authors.lastname: Laktionov
mesh_terms: D001943:Breast Neoplasms / Q000097:blood*; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000097:blood*; D009698:Nucleoproteins / Q000097:blood*
chemical_list: D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009698:Nucleoproteins