title: Proteomic analysis of two divergently responding potato genotypes (Solanum tuberosum L.) following osmotic stress treatment in vitro.
abstract: Starch potatoes (Solanum tuberosum L.) are of interest for production of starch, ethanol, and biopolymers. Due to the predicted increase in drought periods, the breeding of starch potatoes for drought tolerance is essential. This study aims to elucidate the physiological mechanisms that give rise to drought tolerance. Two genotypes contrasting in drought tolerance were compared. We applied osmotic stress which is a known component of drought stress under in vitro conditions. Shoot tips were harvested after 11days of culture on control medium and medium supplied with 0.2M sorbitol. Their proteomes were analyzed using two-dimensional isoelectric focussing sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D-IEF/SDS-PAGE). Of a total of 679 distinct protein spots, 118 and 20 spots with differential abundance were found in the sensitive and the tolerant genotype, respectively, after the application of stress. Using mass spectrometry, the proteins in 100 differentially abundant spots were identified; a majority of these proteins were from the chloroplast. For the sensitive genotype, an increase in the abundance of proteinase inhibitors and their precursors, changes in stress responsive proteins and an altered RNA/DNA-binding response were observed. The differentially abundant spots of the tolerant genotype comprised one chaperone and one hydrogen peroxide detoxifying protein.
  
  
  
  Our findings reveal that the two genotypes have different responses to osmotic stress in terms of protein degradation and reactive oxygen species (ROS) scavenging and production. Our data suggest that the tolerant genotype might adjust to the applied stress more quickly. A comparative temporal analysis might provide further insights into these rapid changes and assist in the development of biomarkers.
authors.affiliation: Leibniz Universität Hannover, Institute of Horticultural Production Systems, Herrenhaeuser Strasse 2, 30419 Hannover, Germany.
authors.forename: Christin
authors.initials: C
authors.lastname: Bündig
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, Corrensstraße 3, 06466 Stadt Seeland, Germany.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Jozefowicz
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, Corrensstraße 3, 06466 Stadt Seeland, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
authors.affiliation: Leibniz Universität Hannover, Institute of Horticultural Production Systems, Herrenhaeuser Strasse 2, 30419 Hannover, Germany. Electronic address: traud.winkelmann@zier.uni-hannover.de.
authors.forename: Traud
authors.initials: T
authors.lastname: Winkelmann
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D013058:Mass Spectrometry; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D011198:Solanum tuberosum / Q000235:genetics*; D013312:Stress, Physiological; D013997:Time Factors
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: In vitro; Mass spectrometry; Osmotic stress; Proteomics; Solanum tuberosum; Stress response