title: The Enzyme-instructed assembly of the core of yeast prion Sup35 to form supramolecular hydrogels.
abstract: Based on the self-assembly capability of the core segment (GNNQQNY) of yeast prion Sup35, we design and synthesis a series of structurally related precursors for enzymatic formation of hydrogels. We found that, with the catalysis of alkaline phosphatase, the precursor becomes a hydrogelator that self-assembles in water to form nanofibers with an average width less than ten nanometers. Interestingly, the introduction of amyloid segment into a cytotoxic precursor (N'ffyp: D-1P) is able to abrogate the cytotoxicity of the precursor, making the resulting peptide to be cell compatible. This work contributes a new insight to the use of enzyme to form cell compatible hydrogels of peptides cross-β spine.
authors.affiliation: 415 South Street, MS 015, Waltham, MA 02453, USA. ; Tel: 781-736-5201.
authors.forename: Dan
authors.initials: D
authors.lastname: Yuan
authors.affiliation: 415 South Street, MS 015, Waltham, MA 02453, USA. ; Tel: 781-736-5201.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Shi
authors.affiliation: 415 South Street, MS 015, Waltham, MA 02453, USA. ; Tel: 781-736-5201.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Du
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 2599 Qianjin St., Changchun 130012, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: 415 South Street, MS 015, Waltham, MA 02453, USA. ; Tel: 781-736-5201.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: 415 South Street, MS 015, Waltham, MA 02453, USA. ; Tel: 781-736-5201.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu