title: Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics".
abstract: Li et al (Research Articles, 28 August 2015, aab3500) purport to present solutions to long-standing challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods' reliability to visualize specimen features at sub-100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany. ssahl@mpibpc.mpg.de fbalzar@mpibpc.mpg.de sjakobs@mpibpc.mpg.de.
authors.forename: Steffen J
authors.initials: SJ
authors.lastname: Sahl
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany. ssahl@mpibpc.mpg.de fbalzar@mpibpc.mpg.de sjakobs@mpibpc.mpg.de.
authors.forename: Francisco
authors.initials: F
authors.lastname: Balzarotti
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Keller-Findeisen
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Leutenegger
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Westphal
authors.affiliation: Laser Laboratory Göttingen, Hans-Adolf-Krebs-Weg 1, 37077 Göttingen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Egner
authors.affiliation: Institut Universitaire en Santé Mentale de Québec, Cellular and Molecular Neuroscience Research Axis, 2601 Chemin de la Canardière, Québec, G1J 2G3, Canada.
authors.forename: Flavie
authors.initials: F
authors.lastname: Lavoie-Cardinal
authors.affiliation: Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany. Technische Universität München, Chair for Biological Imaging, Ismaningerstrasse 22, 81675 München, Germany.
authors.forename: Andriy
authors.initials: A
authors.lastname: Chmyrov
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Grotjohann
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany. University of Göttingen Medical Faculty, Department of Neurology, Robert-Koch-Str. 40, 37075 Göttingen, Germany. ssahl@mpibpc.mpg.de fbalzar@mpibpc.mpg.de sjakobs@mpibpc.mpg.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure*; D004705:Endocytosis; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D015388:Organelles / Q000648:ultrastructure*