title: (-)-Hydroxycitric Acid Nourishes Protein Synthesis via Altering Metabolic Directions of Amino Acids in Male Rats.
abstract: (-)-Hydroxycitric acid (HCA), a major active ingredient of Garcinia Cambogia extracts, had shown to suppress body weight gain and fat accumulation in animals and humans. While, the underlying mechanism of (-)-HCA has not fully understood. Thus, this study was aimed to investigate the effects of long-term supplement with (-)-HCA on body weight gain and variances of amino acid content in rats. Results showed that (-)-HCA treatment reduced body weight gain and increased feed conversion ratio in rats. The content of hepatic glycogen, muscle glycogen, and serum T4 , T3 , insulin, and Leptin were increased in (-)-HCA treatment groups. Protein content in liver and muscle were significantly increased in (-)-HCA treatment groups. Amino acid profile analysis indicated that most of amino acid contents in serum and liver, especially aromatic amino acid and branched amino acid, were higher in (-)-HCA treatment groups. However, most of the amino acid contents in muscle, especially aromatic amino acid and branched amino acid, were reduced in (-)-HCA treatment groups. These results indicated that (-)-HCA treatment could reduce body weight gain through promoting energy expenditure via regulation of thyroid hormone levels. In addition, (-)-HCA treatment could promote protein synthesis by altering the metabolic directions of amino acids. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Han
authors.affiliation: Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Longlong
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Mengling
authors.initials: M
authors.lastname: Peng
authors.affiliation: Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Haitian
authors.initials: H
authors.lastname: Ma
mesh_terms: D000596:Amino Acids; D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D002951:Citrates / Q000737:chemistry*; D029761:Garcinia cambogia / Q000737:chemistry*; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats
chemical_list: D000596:Amino Acids; D002951:Citrates; D010936:Plant Extracts; C007999:hydroxycitric acid
keywords: (-)-hydroxycitric acid; amino acid profile; protein synthesis; rats