title: Evaluation of anti-inflammatory effect of derivative (E)-N-(4-bromophenyl)-2-(thiophen-2-ylmethylene)-thiosemicarbazone.
abstract: The present study aimed to further investigate the anti-inflammatory activity of (E)-N-(4-bromophenyl)-2-(thiophen-2-ylmethylene)-thiosemicarbazone (BTTSC) as well as its antinociceptive effects. The anti-inflammatory activity was assessed using the model of ear edema induced by croton oil-induced and also evaluated in models of paw edema carrageenan-induced and by compound 48/80. Evaluation of the antinociceptive effect was performed through formalin test. In the nociception test induced by formalin the BTTSC showed activity in both phases of the pain, highlighting inflammatory pain, where it was able to reduce the time to paw lick 62.3, 84.30 and 100% at doses of 30, 100 and 300mgkg(-1). The anti-inflammatory activity was performed ear edema induced by croton oil, where none of the doses tested was capable of significantly regress edema. The paw edema carrageenan-induced showed activity compound, where the edema was reduced by 81.9 and 83.2% in the first two times of the experiment at the highest dose used. The paw edema assay induced by compound 48/80, showed that BTTSC after 15min of the inoculum phlogistic agent showed significant reduction of edema with values of 56.53% at a dose of 30mgkg(-1). Our results suggesting this compound exerts its antinociception effects connected with peripheral mechanisms. Furthermore, the compound was able to act in two phases of inflammation carrageenan-induced, highlighting the initial phase. This suggests an action on the early mediators of inflammation. The paw edema assay induced by compound 48/80 confirmed our hypothesis indicating action of the compound via histamine.
authors.affiliation: Universidade Federal de Pernambuco (UFPE), Departamento de Antibióticos, 50670-901 Recife, PE, Brazil.
authors.forename: Jamerson Ferreira
authors.initials: JF
authors.lastname: de Oliveira
authors.affiliation: Universidade Estadual de Campinas (Unicamp), Divisão de Farmacologia e Toxicologia-Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), 13083-970 Campinas, SP, Brazil.
authors.forename: Fabiana Regina
authors.initials: FR
authors.lastname: Nonato
authors.affiliation: Universidade Estadual de Campinas (Unicamp), Divisão de Farmacologia e Toxicologia-Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), 13083-970 Campinas, SP, Brazil.
authors.forename: Rafael Rosolen Teixeira
authors.initials: RRT
authors.lastname: Zafred
authors.affiliation: Universidade Federal de Pernambuco (UFPE), Departamento de Antibióticos, 50670-901 Recife, PE, Brazil.
authors.forename: Nayara Maria Siqueira
authors.initials: NMS
authors.lastname: Leite
authors.affiliation: Universidade Estadual de Campinas (Unicamp), Divisão de Farmacologia e Toxicologia-Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), 13083-970 Campinas, SP, Brazil.
authors.forename: Ana Lúcia Tasca Gois
authors.initials: ALTG
authors.lastname: Ruiz
authors.affiliation: Universidade Estadual de Campinas (Unicamp), Divisão de Farmacologia e Toxicologia-Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), 13083-970 Campinas, SP, Brazil.
authors.forename: João Ernesto
authors.initials: JE
authors.lastname: de Carvalho
authors.affiliation: Universidade Federal do Vale do São Francisco (UNIVASF), Departamento de Medicina, 48607-190 Paulo Afonso, BA, Brazil.
authors.forename: Anekécia Lauro
authors.initials: AL
authors.lastname: da Silva
authors.affiliation: Universidade Estadual da Paraíba (UEPB), Departamento de Farmácia, 58429-500 Paraíba, PB, Brazil.
authors.forename: Ricardo Olímpio
authors.initials: RO
authors.lastname: de Moura
authors.affiliation: Universidade Federal de Pernambuco (UFPE), Departamento de Antibióticos, 50670-901 Recife, PE, Brazil. Electronic address: nenalima.mariadocarmo@gmail.com.
authors.forename: Maria do Carmo
authors.initials: MDC
authors.lastname: Alves de Lima
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002351:Carrageenan; D004487:Edema / Q000473:pathology; D008807:Mice, Inbred BALB C; D059225:Nociception / Q000187:drug effects; D013876:Thiophenes / Q000737:chemistry / Q000494:pharmacology*; D013882:Thiosemicarbazones / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; C000613811:N-(4-bromophenyl)-2-(thiophen-2-ylmethylene)thiosemicarbazone; D013876:Thiophenes; D013882:Thiosemicarbazones; D002351:Carrageenan
keywords: Anti-inflammatory; Antinociceptive; Histamine; Thiosemicarbazone