title: [Dexamethasone increases the expression of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in lung tissues of bronchial asthmatic mice].
abstract: OBJECTIVE
  To investigate the expression of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in the lung tissues of bronchial asthmatic mice and the effect of dexamethasone treatment on its expression.
  
  
  METHODS
  Thirty BALB/c mice were randomly divided into three equal groups: a control group, an asthmatic group and a dexamethasone-treated group. The asthmatic mouse models were established by intraperitoneal injection and inhalation with ovalbumin (OVA). The number of eosinophils (EOS) and lymphocytes (Lym) in bronchoalveolar lavage fluid (BALF) were counted. HE staining was used to observe airway inflammation and remodeling. The mRNA and protein expression of RECK were determined by real-time PCR and immunohistochemistry, respectively.
  
  
  RESULTS
  Compared with the control group and the dexamethasone-treated group, the total cell number and EOS number in the BALF of the asthma group significantly increased. The expression of RECK mRNA in the asthmatic group was significantly lower than that in the control group and the dexamethasone-treated group. Immunohistochemistry showed that RECK was mainly expressed in the airway epithelial cells and inflammatory cells. RECK protein expression was highest in the control group and lowest in the asthmatic group.
  
  
  CONCLUSIONS
  Dexamethasone can increase the expression of RECK in the lung tissues of asthmatic mice.
authors.affiliation: Department of Respiratory Diseases, Third Hospital of Guangzhou Medical University, Guangzhou 510150, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Respiratory Diseases, Third Hospital of Guangzhou Medical University, Guangzhou 510150, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Respiratory Diseases, Third Hospital of Guangzhou Medical University, Guangzhou 510150, China. *Corresponding author, E-mail: weilp8@hotmail.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Prevention and Health Care, Third Hospital of Guangzhou Medical University, Guangzhou 510150, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Pathology, Third Hospital of Guangzhou Medical University, Guangzhou 510150, China.
authors.forename: Hanzhen
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Severe Tuberculosis, Guangzhou Chest Hospital, Guangzhou 510000, China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Li
authors.affiliation: Second Department of Internal Medicine, Shenzhen Third People' s Hospital, Shenzhen 518112, China.
authors.forename: Peifen
authors.initials: P
authors.lastname: Chen
authors.affiliation: Laboratory of Cardiovascular Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Lai
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D058851:GPI-Linked Proteins / Q000032:analysis / Q000235:genetics*; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D058851:GPI-Linked Proteins; D012333:RNA, Messenger; C492025:Reck protein, mouse; D003907:Dexamethasone