title: Tissue in Cube: In Vitro 3D Culturing Platform with Hybrid Gel Cubes for Multidirectional Observations.
abstract: An in vitro 3D culturing platform enabling multidirectional observations of 3D biosamples is presented. The 3D structure of biosamples can be recognized without fluorescence. The cubic platform employs two types of hydrogels that are compatible with conventional culture dishes or well plates, facilitating growth in culture, ease of handling, and viewing at multiple angles.
authors.affiliation: Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku Sakai-shi, Osaka, 599-8570, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Hagiwara
authors.affiliation: Department of Biological Functions Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, 808-0196, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kawahara
authors.affiliation: Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku Sakai-shi, Osaka, 599-8570, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Nobata
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D018920:Coculture Techniques; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*
chemical_list: D020100:Hydrogels
keywords: 3D culture; 3D imaging; development; multidirectional observation; organoid