title: Intense Resistance Exercise Promotes the Acute and Transient Nuclear Translocation of Small Ubiquitin-Related Modifier (SUMO)-1 in Human Myofibres.
abstract: Protein sumoylation is a posttranslational modification triggered by cellular stress. Because general information concerning the role of small ubiquitin-related modifier (SUMO) proteins in adult skeletal muscle is sparse, we investigated whether SUMO-1 proteins will be subjected to time-dependent changes in their subcellular localization in sarcoplasmic and nuclear compartments of human type I and II skeletal muscle fibers in response to acute stimulation by resistance exercise (RE). Skeletal muscle biopsies were taken at baseline (PRE), 15, 30, 60, 240 min and 24 h post RE from 6 male subjects subjected to a single bout of one-legged knee extensions. SUMO-1 localization was determined via immunohistochemistry and confocal laser microscopy. At baseline SUMO-1 was localized in perinuclear regions of myonuclei. Within 15 and up to 60 min post exercise, nuclear SUMO-1 localization was significantly increased (p < 0.01), declining towards baseline levels within 240 min post exercise. Sarcoplasmic SUMO-1 localization was increased at 15 min post exercise in type I and up to 30 min post RE in type II myofibres. The changing localization of SUMO-1 proteins acutely after intense muscle contractions points to a role for SUMO proteins in the acute regulation of the skeletal muscle proteome after exercise.
authors.affiliation: Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, 50933 Cologne, Germany. Gehlert@dshs-koeln.de.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Gehlert
authors.affiliation: Institute for Dental Research and Oral Musculoskeletal Biology, University of Cologne, 50935 Cologne, Germany. aka33@uni-koeln.de.
authors.forename: Franz Josef
authors.initials: FJ
authors.lastname: Klinz
authors.affiliation: Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, 50933 Cologne, Germany. williwictim@googlemail.com.
authors.forename: Lena
authors.initials: L
authors.lastname: Willkomm
authors.affiliation: Outpatient Clinic for Sports Traumatology and Public health Consultation, German Sport University Cologne, 50933 Cologne, Germany. t.schiffer@dshs-koeln.de.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schiffer
authors.affiliation: Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, 50933 Cologne, Germany. suhr@dshs-koeln.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Suhr
authors.affiliation: Institute of Cardiovascular Research and Sport Medicine, Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, 50933 Cologne, Germany. w.bloch@dshs-koeln.de.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Bloch
mesh_terms: D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism; D015444:Exercise; D006801:Humans; D007150:Immunohistochemistry; D034904:Lamin Type A / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000473:pathology; D025842:SUMO-1 Protein / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D055815:Young Adult
chemical_list: D034904:Lamin Type A; D025842:SUMO-1 Protein
keywords: SUMO-1; acute response; nuclear translocation; resistance exercise; skeletal muscle adaptation; sumoylation