title: Smart CuS Nanoparticles as Peroxidase Mimetics for the Design of Novel Label-Free Chemiluminescent Immunoassay.
abstract: In the present work, a novel label-free chemiluminescent (CL) immunoassay method was designed by employing smart CuS nanoparticles (CuSNPs) as peroxidase mimetics. The CuSNPs were synthesized through a simple coprecipitation method, and showed high catalytic activity and stability. This efficient label-free CL immunoassay could be easily achieved through a simple strategy. First, CuSNPs dispersed in chitosan were modified on the epoxy-functionalized glass slide to form a solid CL signal interface. Streptavidin was then used to functionalize CuSNPs to capture the biotinylated antibody, further producing a sensing interface. After online incubation with antigen molecules, the formed antibody-antigen complex on the biosensing substrate could prevent the diffusion channel of CL substrate toward the signal interface, and restrained the mimic enzyme-catalyzed CL reaction, finally resulting in the decrease of CL signals of the assay system. Compared to the label-based CL immunoassay, the proposed label-free assay mode is more simple, cheap and fast. Using a model analyte alpha-fetoprotein, the label-free CL immunoassay method had a linear range of 0.1-60 ng/mL and a low detection limit of 0.07 ng/mL. Moreover, the peroxidase mimetic-based label-free CL immunoassay system showed good specificity, acceptable repeatability, and good accuracy. The study provided a promising strategy for the development of highly efficient label-free CL immunoassay system.
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University , Yangzhou 225002, P.R. China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University , Yangzhou 225002, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University , Yangzhou 225002, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University , Yangzhou 225002, P.R. China.
authors.forename: Mimi
authors.initials: M
authors.lastname: Lu
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University , Yangzhou 225002, P.R. China.
authors.forename: Zhikang
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University , Yangzhou 225002, P.R. China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D013440:Sulfides; D000509:alpha-Fetoproteins; D003300:Copper; D009195:Peroxidase
keywords: CuS nanoparticles; chemiluminescence; immunoassay; label-free; peroxidase mimetics; tumor markers