title: Crystal structures of Staphylococcal SaeR reveal possible DNA-binding modes.
abstract: Two-component system SaeRS of Staphylococcus regulates virulence factor expression through phosphorylation of the DNA-binding regulator SaeR by the sensor histidine kinase SaeS. Here crystal structures of the DNA-binding domain (DBD) of SaeR from two Staphylococcal species Staphylococcus epidermidis and Staphylococcus aureus were determined and showed similar folds. Analyzing the DNA binding activity of three mutants of SeSaeR, we observed that Thr217 is important in binding to the phosphate group of DNA and Trp219 may interact with the base pairs. Additionally, the tandem arrangement of DBD may represent a possible way for SaeR oligomerization on DNA.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: Tzu-Ping
authors.initials: TP
authors.lastname: Ko
authors.affiliation: School of Biomedical Sciences, Chung Shan Medical University, No.110, Sec.1, Chien-Kuo N. Rd., Taichung, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, No.110, Sec.1, Chien-Kuo N. Rd., Taichung, Taiwan.
authors.forename: Cheng-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: Tung-Ju
authors.initials: TJ
authors.lastname: Hsieh
authors.affiliation: Department of Biotechnology, Hungkuang University, No.1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan.
authors.forename: Sheng-Chia
authors.initials: SC
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, Hungkuang University, No.1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan.
authors.forename: Yu-Ren
authors.initials: YR
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, Hungkuang University, No.1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan.
authors.forename: Chia-Shin
authors.initials: CS
authors.lastname: Yang
authors.affiliation: Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Hao-Cheng
authors.initials: HC
authors.lastname: Kuo
authors.affiliation: Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Wen-Lung
authors.initials: WL
authors.lastname: Wang
authors.affiliation: Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Tzu-Hung
authors.initials: TH
authors.lastname: Hsiao
authors.affiliation: Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Ching-Heng
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Biotechnology, Hungkuang University, No.1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan. Electronic address: chyeah6599@sunrise.hk.edu.tw.
authors.forename: Yeh
authors.initials: Y
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D001665:Binding Sites; D003198:Computer Simulation; D003461:Crystallography / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry* / Q000648:ultrastructure*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000648:ultrastructure; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C433805:SaeR protein, Staphylococcus aureus; D014157:Transcription Factors
keywords: Crystal structure; SaeR; Staphylococcus; Two-component system