title: Genotoxic effects of cadmium in human head and neck cell line SQ20B.
abstract: As cadmium may be involved in the etiology of head and neck cancers, we investigated in the present work, the cytotoxic and genotoxic effects of Cd on human larynx cells. SQ20B cells were exposed to 25 and 50 μM Cd for 48 and 72 h. Results showed a dose-dependent decrease in cell viability, especially after 48 h, associated with mitochondria alterations as showed by transmission electronic microscopy. Surprisingly, the flow cytometry shows that the cells treated with Cd have a normal proliferative cycle like the untreated cell especially in G1 or G2 phase of cell cycle. DNA damages were investigated by comet assay and immunofluorescence for gamma layer of the H2AX (g-H2AX) foci formation. Results show a strong induction of DNA double-strand breaks after Cd exposure. Overall, our results demonstrate the cytotoxicity and genotoxicity of Cd in human larynx cells and support the view that Cd could be an etiologic factor of head and neck cancers.
authors.affiliation: Unit of Marine and Environmental Toxicology, UR 09-03, IPEIS, Sfax University, BP 1172, 3018, Sfax, Tunisia. fattoumtrabelsi@yahoo.fr.
authors.forename: Fatma
authors.initials: F
authors.lastname: Trabelsi
authors.affiliation: Unit of Marine and Environmental Toxicology, UR 09-03, IPEIS, Sfax University, BP 1172, 3018, Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Khlifi
authors.affiliation: Normandie Université, Caen, France.|UNICAEN, CMAbio, SFR ICORE, 14032, Caen, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Goux
authors.affiliation: Normandie Université, Caen, France.|UMR-S 1075 Inserm/Unicaen - COMETE, 14032, Caen, France.
authors.forename: Marilyne
authors.initials: M
authors.lastname: Guillamin
authors.affiliation: Unit of Marine and Environmental Toxicology, UR 09-03, IPEIS, Sfax University, BP 1172, 3018, Sfax, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Hamza-Chaffai
authors.affiliation: Normandie Université, Caen, France.|UNICAEN, ABTE E4651, 14032, Caen, France.|Centre François Baclesse, avenue Général Harris, BP5026, 14076, Caen CEDEX-05, France.
authors.forename: François
authors.initials: F
authors.lastname: Sichel
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D006258:Head and Neck Neoplasms / Q000139:chemically induced; D006801:Humans; D009153:Mutagens / Q000633:toxicity*
chemical_list: D009153:Mutagens; D002104:Cadmium
keywords: Cadmium chloride; Comet assay; DNA double-strand breaks; Genotoxicity; Head and neck cancers; Histone H2AX