title: Cellufine sulfate column chromatography as a simple, rapid, and effective method to purify dengue virus.
abstract: Conventional method to purify/concentrate dengue virus (DENV) is time-consuming with low virus recovery yield. Herein, we applied cellufine sulfate column chromatography to purify/concentrate DENV based on the mimicry between heparan sulfate and DENV envelope protein. Comparative analysis demonstrated that this new method offered higher purity (as determined by less contamination of bovine serum albumin) and recovery yield (as determined by greater infectivity). Moreover, overall duration used for cellufine sulfate column chromatography to purify/concentrate DENV was approximately 1/20 of that of conventional method. Therefore, cellufine sulfate column chromatography serves as a simple, rapid, and effective alternative method for DENV purification/concentration.
authors.affiliation: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, and Center for Research in Complex Systems Science, Mahidol University, Bangkok 10700, Thailand.
authors.forename: Rattiyaporn
authors.initials: R
authors.lastname: Kanlaya
authors.affiliation: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, and Center for Research in Complex Systems Science, Mahidol University, Bangkok 10700, Thailand. Electronic address: thongboonkerd@dr.com.
authors.forename: Visith
authors.initials: V
authors.lastname: Thongboonkerd
mesh_terms: D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D003715:Dengue / Q000175:diagnosis / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification*; D011108:Polymers; D014759:Viral Envelope Proteins / Q000302:isolation & purification
chemical_list: D011108:Polymers; D014759:Viral Envelope Proteins; C080965:sulfate cellufine
keywords: Cellufine sulfate; Dengue; Envelope; Infectivity; Purification; Sucrose gradient