title: miR-195 inhibits tumor growth and angiogenesis through modulating IRS1 in breast cancer.
abstract: Angiogenesis has been found as an attractive target for drug therapy as it is necessary for tumor growth. Accumulating evidences show that microRNAs (miRNAs), which are a group of highly conserved, single-stranded, short non-coding RNAs, play important roles through directly targeting angiogenic factors and protein kinases. The purpose of this study is to investigate the role of miR-195 in breast cancer development and angiogenesis through targeting IRS1. We show that miR-195 is inversely related with Insulin receptor substrate 1 (IRS1) in both breast cancer cells and breast cancer tissues. Induction of miR-195 could suppress IRS1 protein expression through binding to its 3'UTR regions either by transfection with miR-195 oligo or by infection with lentivirus encoding miR-195 gene. Moreover, re-expression of IRS1 reverses miR-195-mediated repression of tumor cell growth and miR-195 inhibits tumor angiogenesis through suppressing IRS1-VEGF axis. These data suggest that miR-195 mimics are potential therapeutic agents for breast cancer diagnose.
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zou
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China; Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China; Brain Tumor Center, Neurosurgery Division, Faculty of Medicine, PWH, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Marie C
authors.initials: MC
authors.lastname: Lin
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China; CAS-MPG Partner Institute and Key Lab for Computational Biology, SIBS, CAS, Shanghai, China.
authors.forename: Andreas
authors.initials: A
authors.lastname: Dress
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China; Randall Division of Cell and Molecular Biophysics, New Hunt's House, King's College of London, Guy's Campus, London SE1 1UL, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Wardle
authors.affiliation: State Key Lab of Reproductive Medicine, and Department of Pathology, Cancer Center, Nanjing Medical University, Nanjing, China; Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA.
authors.forename: Bing-Hua
authors.initials: BH
authors.lastname: Jiang
authors.affiliation: Department of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China. Electronic address: lhlai@bio.ecnu.edu.cn.
authors.forename: Lihui
authors.initials: L
authors.lastname: Lai
mesh_terms: D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000098:blood supply* / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002645:Chickens; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: D055504:Insulin Receptor Substrate Proteins; C552722:MIRN195 microRNA, human; D035683:MicroRNAs
keywords: Angiogenesis; Breast cancer; Insulin receptor substrate 1; MiR-195