title: Research on a novel chitosan microsphere/scaffold system by double crosslinkers.
abstract: RhBMP-2 has shown great promise for the reconstruction of teeth segmental bone defects due to its osteoinductive properties. But the application of rhBMP-2 is limited by its weak drug controled release. It is usually loaded in a Chitosan Microspheres (CMs) delivery system with excess single cross-linker and then removed before practice. In this study, cross-linkers were replaced with RhBMP-2 which contains vanillin and vitriolic acid, and thus CMs were developed. The materials were studied by SEM, FTIR and drug release experiments. It showed an ideal releasing profile and excellent osteoconductive and osteoinductive performance in the delivery system. Therefore, designing biomaterials with a controllable delivery system composite and releasing profile of rhBMP-2 are critical for applications of bone regeneration and tissue engineering.
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Tai
authors.forename: Fengyu
authors.initials: F
authors.lastname: Han
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xi
authors.forename: Meili
authors.initials: M
authors.lastname: Liu
authors.forename: Qianfan
authors.initials: Q
authors.lastname: Wu
authors.forename: Yubo
authors.initials: Y
authors.lastname: Fan
mesh_terms: D055396:Bone Morphogenetic Protein 2; D001861:Bone Regeneration; D048271:Chitosan; D008863:Microspheres; D011994:Recombinant Proteins; D054457:Tissue Scaffolds
chemical_list: D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D048271:Chitosan