title: Quantitative Proteomics Reveals the Flooding-Tolerance Mechanism in Mutant and Abscisic Acid-Treated Soybean.
abstract: Flooding negatively affects the growth of soybean, and several flooding-specific stress responses have been identified; however, the mechanisms underlying flooding tolerance in soybean remain unclear. To explore the initial flooding tolerance mechanisms in soybean, flooding-tolerant mutant and abscisic acid (ABA)-treated plants were analyzed. In the mutant and ABA-treated soybeans, 146 proteins were commonly changed at the initial flooding stress. Among the identified proteins, protein synthesis-related proteins, including nascent polypeptide-associated complex and chaperonin 20, and RNA regulation-related proteins were increased in abundance both at protein and mRNA expression. However, these proteins identified at the initial flooding stress were not significantly changed during survival stages under continuous flooding. Cluster analysis indicated that glycolysis- and cell wall-related proteins, such as enolase and polygalacturonase inhibiting protein, were increased in abundance during survival stages. Furthermore, lignification of root tissue was improved even under flooding stress. Taken together, these results suggest that protein synthesis- and RNA regulation-related proteins play a key role in triggering tolerance to the initial flooding stress in soybean. Furthermore, the integrity of cell wall and balance of glycolysis might be important factors for promoting tolerance of soybean root to flooding stress during survival stages.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan.|National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Yin
authors.affiliation: Graduate School of Life and Food Sciences, Niigata University , Niigata 950-2181, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Nishimura
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.
authors.forename: Makita
authors.initials: M
authors.lastname: Hajika
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan.|National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000222:Adaptation, Physiological / Q000187:drug effects*; D055868:Floods; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D010940:Plant Proteins / Q000032:analysis; D018517:Plant Roots; D014176:Protein Biosynthesis; D040901:Proteomics / Q000379:methods*; D013025:Glycine max / Q000737:chemistry* / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid
keywords: abscisic acid; flooding stress; mutant; root; soybean; stress tolerance