title: Five 2-(2-Phenylethyl)chromones from Sodium Chloride-Elicited Aquilaria sinensis Cell Suspension Cultures.
abstract: Five 2-(2-phenylethyl)chromones including a new one, (5S,6R,7S,8R)-5,8-dichloro-6,7-dihydroxy-2-phenylethyl-5,6,7,8-tetrahydro-4H-chromen-4-one (1), and four known ones (2-5), were isolated from 150 mM NaCl-elicited Aquilaria sinensis cell suspension cultures. In addition, three feruloyl amides (6-8), six nucleosides (9-14), (+)-syringaresinol (15), indole-3-carboxaldehyde (16), and two glycosides (17-18) were also obtained. The structures were unambiguously identified by analysis of their UV, IR, NMR, and HRESIMS data. The absolute configuration of the new 2-(2-phenylethyl)chromone (1) was established by a dimolybdenum tetraacetate-induced circular dichroism experiment. Compared to un-elicited cell lines, the appearance of 2-(2-phenylethyl)chromones in NaCl-treated cells occurred on the 3rd and 5th days of their treatment. 2-(2-Phenylethyl)chromones, feruloyl amides, nucleosides, and lignins have been reported to be closely related to plant defense; therefore, the identification of these compounds from NaCl-elicited A. sinensis cell suspension cultures would be useful for further exploring the mechanism of agarwood formation.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. zhangzx07@163.com.|School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. zhangzx07@163.com.
authors.forename: Zhongxiu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. wangxhui2014@163.com.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. yangwanqing511@163.com.|School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. yangwanqing511@163.com.
authors.forename: Wanqing
authors.initials: W
authors.lastname: Yang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. wjwangjuan2012@163.com.|School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. wjwangjuan2012@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. suerzong@163.com.|School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. suerzong@163.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Su
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. fcliuxiao@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. drlj666@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. yunfang.zhao@163.com.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. shishepo@163.com.
authors.forename: Shepo
authors.initials: S
authors.lastname: Shi
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. pengfeitu@163.com.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis; D012965:Sodium Chloride / Q000494:pharmacology; D029645:Thymelaeaceae / Q000737:chemistry / Q000166:cytology*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010936:Plant Extracts; D012965:Sodium Chloride
keywords: 2-(2-phenylethyl)chromone; Aquilaria sinensis; agarwood; cell suspension cultures; induced circular dichroism; sodium chloride elicitation