title: Development of a commercial scale process for production of 1,4-butanediol from sugar.
abstract: A sustainable bioprocess for the production of 1,4-butanediol (BDO) from carbohydrate feedstocks was developed. BDO is a chemical intermediate that goes into a variety of products including automotive parts, electronics, and apparel, and is currently manufactured commercially through energy-intensive petrochemical processes using fossil raw materials. This review highlights the development of an Escherichia coli strain and an overall process that successfully performed at commercial scale for direct production of bio-BDO from dextrose. Achieving such high level performance required an integrated technology platform enabling detailed engineering of enzyme, pathway, metabolic network, and organism, as well as development of effective fermentation and downstream recovery processes.
authors.affiliation: Genomatica, Inc., 4757 Nexus Center Drive, San Diego, CA 92121, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Burgard
authors.affiliation: Genomatica, Inc., 4757 Nexus Center Drive, San Diego, CA 92121, USA. Electronic address: mburk@genomatica.com.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Burk
authors.affiliation: Genomatica, Inc., 4757 Nexus Center Drive, San Diego, CA 92121, USA.
authors.forename: Robin
authors.initials: R
authors.lastname: Osterhout
authors.affiliation: Genomatica, Inc., 4757 Nexus Center Drive, San Diego, CA 92121, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Van Dien
authors.affiliation: Genomatica, Inc., 4757 Nexus Center Drive, San Diego, CA 92121, USA.
authors.forename: Harry
authors.initials: H
authors.lastname: Yim
mesh_terms: D000818:Animals; D002072:Butylene Glycols / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000502:physiology*; D003132:Commerce; D004345:Drug Industry / Q000191:economics / Q000379:methods / Q000639:trends; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways; D013395:Sucrose / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D013395:Sucrose; C039681:1,4-butanediol; D005947:Glucose