title: Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing.
abstract: Tibetan Chickens should have unique gastrointestinal microbiota because of their particular habitats. Thus, the aim of this study was to investigate the cecal microbiota of Tibetan Chickens from five typical high-altitude regions of China. Lohmann egg-laying hens (LMs) and Daheng broiler chickens (DHs) were chosen as controls. The cecal bacterial populations of Tibetan Chickens were surveyed by high-throughput sequencing (HTS) of the bacterial 16S rRNA hypervariable region V3-V4 (16S rRNAV3-V4) combined with community-fingerprinting analysis of the 16S rRNA gene based on polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results revealed that the majority of cecal microbiota differed between the Tibetan Chicken and LM/DH. The microbial communities in the cecum were composed of 16 phyla, 28 classes, 36 orders, 57 families, 101 genera, and 189 species. Represented phyla were Bacteroidetes (>47%), Firmicutes (>18.8%), Spirochaetae (>0.3%), and Proteobacteria (>0.4%). Bacteroides and the RC9 gut group were the two most abundant genera. There were relatively more Christensenellaceae, Subdoligranulum, Spirochaeta, and Treponema in Tibetan Chickens, whereas there were more Phascolarctobacterium, Faecalibacterium, Megamonas, and Desulfovibrio in LMs and DHs. The cecal microbiota of Tibetan Chicken have slightly diverged due to exposure to different geographic environments. Differences in the intestinal bacterial communities of Tibetan Chicken and LM/DH were noted.
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Poultry Science, Academy of Sichuan Animal Husbandry Research, Chengdu, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Institute of Poultry Science, Academy of Sichuan Animal Husbandry Research, Chengdu, China.
authors.forename: Chaowu
authors.initials: C
authors.lastname: Yang
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Bingcun
authors.initials: B
authors.lastname: Ma
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Lei
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Xu
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Anyun
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Men
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, College of Life Sciences, Sichuan University, Chengdu, China. whongning@163.com.
authors.forename: Hongning
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002432:Cecum / Q000382:microbiology*; D002645:Chickens; D002681:China; D058645:Denaturing Gradient Gel Electrophoresis; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D005843:Geography; D059014:High-Throughput Nucleotide Sequencing; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017423:Sequence Analysis, RNA; D018609:Tibet
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Cecal microbiota; DGGE; Daheng broiler chickens; Lohmann egg-laying hens; Tibetan Chicken; high-throughput sequencing