title: Asenapine modulates nitric oxide release and calcium movements in cardiomyoblasts.
abstract: OBJECTIVE
  To examine the effects of asenapine on nitric oxide (NO) release and Ca(2+) transients in H9C2 cell line, which were either subjected to peroxidation or not.
  
  
  METHODS
  H9C2 were treated with asenapine alone or in presence of intracellular kinase blockers, serotoninergic and dopaminergic antagonists, and voltage Ca(2+) channels inhibitors. Experiments were also performed in H9C2 treated with hydrogen peroxide. NO release and intracellular Ca(2+) were measured through specific probes.
  
  
  RESULTS
  In H9C2, asenapine differently modulated NO release and Ca(2+) movements depending on peroxidative condition. The Ca(2+) pool mobilized by asenapine mainly originated from the extracellular space and was slightly affected by thapsigargin. Moreover, the effects of asenapine were reduced or prevented by kinases blockers, dopaminergic and serotoninergic receptors inhibitors, and voltage Ca(2+) channels blockers.
  
  
  CONCLUSIONS
  On the basis of our findings, we can conclude that asenapine by interacting with its specific receptors, exerts dual effects on NO release and Ca(2+) homeostasis in H9C2; this would be of particular clinical relevance when considering their role in cardiac function modulation.
authors.affiliation: Department of Translational Medicine, Laboratory of Physiology and Experimental Surgery, University of Eastern Piedmont "A. Avogadro", Via Solaroli 17, I-28100, Novara, Italy; Azienda Ospedaliera Universitaria Maggiore Della Carità, Corso Mazzini 36, Novara, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Grossini
authors.affiliation: Azienda Ospedaliera Universitaria Maggiore Della Carità, Corso Mazzini 36, Novara, Italy; Department of Translational Medicine, Psichiatric Unit, University of Eastern Piedmont "A. Avogadro", Via Solaroli 17, I-28100, Novara, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Gramaglia
authors.affiliation: Department of Translational Medicine, Laboratory of Physiology and Experimental Surgery, University of Eastern Piedmont "A. Avogadro", Via Solaroli 17, I-28100, Novara, Italy; Azienda Ospedaliera Universitaria Maggiore Della Carità, Corso Mazzini 36, Novara, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Farruggio
authors.affiliation: Azienda Ospedaliera Universitaria Maggiore Della Carità, Corso Mazzini 36, Novara, Italy; Department of Translational Medicine, Psichiatric Unit, University of Eastern Piedmont "A. Avogadro", Via Solaroli 17, I-28100, Novara, Italy.
authors.forename: Lara
authors.initials: L
authors.lastname: Camillo
authors.affiliation: Department of Translational Medicine, Laboratory of Physiology and Experimental Surgery, University of Eastern Piedmont "A. Avogadro", Via Solaroli 17, I-28100, Novara, Italy; Azienda Ospedaliera Universitaria Maggiore Della Carità, Corso Mazzini 36, Novara, Italy.
authors.forename: David
authors.initials: D
authors.lastname: Mary
authors.affiliation: Department of Translational Medicine, Laboratory of Physiology and Experimental Surgery, University of Eastern Piedmont "A. Avogadro", Via Solaroli 17, I-28100, Novara, Italy; Azienda Ospedaliera Universitaria Maggiore Della Carità, Corso Mazzini 36, Novara, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vacca
authors.affiliation: Azienda Ospedaliera Universitaria Maggiore Della Carità, Corso Mazzini 36, Novara, Italy; Department of Translational Medicine, Psichiatric Unit, University of Eastern Piedmont "A. Avogadro", Via Solaroli 17, I-28100, Novara, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Zeppegno
keywords: Antipsychotics; calcium transients; dopaminergic receptors; serotoninergic receptors; voltage channels