title: Purification, gene cloning, and characterization of γ-butyrobetainyl CoA synthetase from Agrobacterium sp. 525a.
abstract: The report is the first of purification, overproduction, and characterization of a unique γ-butyrobetainyl CoA synthetase from soil-isolated Agrobacterium sp. 525a. The primary structure of the enzyme shares 70-95% identity with those of ATP-dependent microbial acyl-CoA synthetases of the Rhizobiaceae family. As distinctive characteristics of the enzyme of this study, ADP was released in the catalytic reaction process, whereas many acyl CoA synthetases are annotated as an AMP-forming enzyme. The apparent Km values for γ-butyrobetaine, CoA, and ATP were, respectively, 0.69, 0.02, and 0.24 mM.
authors.affiliation: a United Graduate School of Agricultural Sciences , Tottori University , Tottori , Japan.|b Food Development Research Laboratory , Tottori Institute of Industrial Technology , Sakaiminato , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujimitsu
authors.affiliation: c Faculty of Agriculture, Department of Agricultural, Biological, and Environmental Sciences , Tottori University , Tottori , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumoto
authors.affiliation: c Faculty of Agriculture, Department of Agricultural, Biological, and Environmental Sciences , Tottori University , Tottori , Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Takubo
authors.affiliation: c Faculty of Agriculture, Department of Agricultural, Biological, and Environmental Sciences , Tottori University , Tottori , Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Fukui
authors.affiliation: c Faculty of Agriculture, Department of Agricultural, Biological, and Environmental Sciences , Tottori University , Tottori , Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Okada
authors.affiliation: d Department of Food Science and Nutrition, College of Food and Agricultural Sciences , King Saud University , Riyadh , KSA.
authors.forename: Isam A
authors.initials: IA
authors.lastname: Mohamed Ahmed
authors.affiliation: c Faculty of Agriculture, Department of Agricultural, Biological, and Environmental Sciences , Tottori University , Tottori , Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Arima
authors.affiliation: c Faculty of Agriculture, Department of Agricultural, Biological, and Environmental Sciences , Tottori University , Tottori , Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Mori
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000737:chemistry / Q000378:metabolism*; D060054:Agrobacterium / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002331:Carnitine / Q000737:chemistry / Q000378:metabolism*; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; C413606:gamma-butyrobetainyl-CoA; D001622:Betaine; C002889:gamma-butyrobetaine; D000244:Adenosine Diphosphate; D003066:Coenzyme A Ligases; D002331:Carnitine
keywords: 4-trimethylaminobutyrate-CoAligase; ADP-forming acyl CoA synthetase; l-carnitine; γ-butyrobetaine; γ-butyrobetaine-CoA ligase