title: Cytotoxic and Antibiotic Cyclic Pentapeptide from an Endophytic Aspergillus tamarii of Ficus carica.
abstract: A new cyclic pentapeptide, disulfide cyclo-(Leu-Val-Ile-Cys-Cys) (1), named malformin E, together with 13 known cyclic dipeptides, was isolated from the culture broth of endophytic fungus FR02 from the roots of Ficus carica. The strain FR02 was identified as Aspergillus tamarii on the basis of morphological characteristics and molecular analyses of internal transcribed spacer (ITS). Their structures were determined by the combination of 1D and 2D NMR spectroscopy, HRMS (ESI), UV, and Marfey's analysis. Compound 1 exhibited strong cytotoxic activities against human cancer cell strains MCF-7 and A549 with IC50 values of 0.65 and 2.42 μM, respectively. It also displayed remarkable antimicrobial activities against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Penicillium chrysogenum, Candida albicans, and Fusarium solani with MIC values of 0.91, 0.45, 1.82, 0.91, 3.62, 7.24, and 7.24 μM, respectively.
authors.affiliation: Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education. Shaanxi University of Science & Technology , Xi'an 710021, Shaanxi, China.
authors.forename: Yang-Min
authors.initials: YM
authors.lastname: Ma
authors.affiliation: Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education. Shaanxi University of Science & Technology , Xi'an 710021, Shaanxi, China.
authors.forename: Xi-Ai
authors.initials: XA
authors.lastname: Liang
authors.affiliation: Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education. Shaanxi University of Science & Technology , Xi'an 710021, Shaanxi, China.|College of Agronomy and Life Science, Shanxi Datong University , Datong 037009, Shanxi, China.
authors.forename: Hong-Chi
authors.initials: HC
authors.lastname: Zhang
authors.affiliation: College of Agronomy and Life Science, Shanxi Datong University , Datong 037009, Shanxi, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000378:metabolism*; D030681:Ficus / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013057:Spectrum Analysis
chemical_list: D000900:Anti-Bacterial Agents; D010456:Peptides, Cyclic
keywords: Aspergillus tamarii; antimicrobial activity; cyclopeptide; cytotoxicity; malformin E