title: The role of shrimp miR-965 in virus infection.
abstract: RNAi, mediated by microRNAs (miRNAs), has attracted increasing attention for its important role in cross-talk between host and virus. However, the role of host miRNA in the virus infection in vivo has not been intensively investigated. In this study, the effects of a shrimp miRNA (miR-965) on the white spot syndrome virus (WSSV) infection were characterized. The results indicated that the expression of miR-965 was significantly upregulated in shrimp in response to the WSSV challenge, suggesting its involvement in the virus infection. The miR-965 silencing led to significant increases of WSSV copies and virus-infected shrimp mortality, while the miR-965 overexpression resulted in the decreased WSSV copies and virus-infected shrimp mortality, indicating that miR-965 played a negative role in the WSSV infection. The further data revealed that miR-965 inhibited the virus infection by targeting the viral wsv240 gene, an important gene required for the WSSV infection in shrimp. The results demonstrated that miR-965 could promote the shrimp phagocytosis against virus infection by targeting the shrimp ATG5 (autophagy related 5) gene. Therefore, our findings presented novel evidence to better understand the anfractuous host-virus interactions in vivo.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Le
authors.initials: L
authors.lastname: Shu
authors.affiliation: Institute of Health Sciences, Anhui University, People's Republic of China. Electronic address: lichangrun@ahu.edu.cn.
authors.forename: Changrun
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: zxb0812@zju.edu.cn.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000276:immunology* / Q000821:virology*; D034622:RNA Interference; D011897:Random Allocation; D015854:Up-Regulation; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D035683:MicroRNAs
keywords: Phagocytosis; Shrimp; WSSV; miR-965