title: Clostridium Butyricum CGMCC0313.1 Modulates Lipid Profile, Insulin Resistance and Colon Homeostasis in Obese Mice.
abstract: Obesity is associated with a cluster of metabolic disorders and systemic low-grade inflammation involving multiple organs. Recent findings have suggested that intestine is a key organ altered in response to high fat diet (HFD) feeding. Probiotics mainly lactobacillus strains have earlier been implicated in alleviating metabolic disorders. Here we aimed to examine the effects of a naturally occurring butyrate-producing probiotic clostridium butyricum CGMCC0313.1 (CB0313.1) in limiting the development of HFD-induced obesity. Mice treated with CB0313.1 exhibited reduced lipid accumulation in liver and serum, lower circulating insulin levels and improved glucose tolerance and insulin sensitivity. Furthermore, CB0313.1 administration reversed the HFD-induced colonic inflammation as evidenced by reduced tumor necrosis factor (TNF)-α level and increases the interleukin (IL)-10 and IL-22 levels in colon tissue. Additionally to colonic inflammation, CB0313.1 also reduced the colon permeability by upregulating the tight junction (TJ) proteins (claudin-1 and occludin) and contributed to a decreased circulating endotoxin level. In colon content, CB0313.1 administration restored the reduced production of butyrate and other short chain fatty acids (SCFAs) caused by HFD feeding. In adipose tissue, lower transcriptional levels of pro-inflammatory TNF-α, IL-6, IL-1β and monocyte chemotactic protein (MCP)-1 in adipose tissue were observed in CB0313.1-treated mice. Collectively, our data demonstrated that CB0313.1, targeting colon inflammation and permeability, ameliorated HFD-induced obesity, insulin resistance as well as adipose inflammation.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology and Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, P. R. China.
authors.forename: Haixiao
authors.initials: H
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology and Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, P. R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology and Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, P. R. China.
authors.forename: Yong Q
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology; D062445:Claudin-1 / Q000235:genetics / Q000276:immunology; D046972:Clostridium butyricum / Q000276:immunology*; D003106:Colon / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D004731:Endotoxins / Q000097:blood; D005232:Fatty Acids, Volatile / Q000097:blood*; D005786:Gene Expression Regulation; D005951:Glucose Tolerance Test; D006706:Homeostasis / Q000187:drug effects / Q000276:immunology; D007113:Immunity, Innate; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000276:immunology*; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000178:diet therapy* / Q000209:etiology / Q000276:immunology / Q000473:pathology; D062793:Occludin / Q000235:genetics / Q000276:immunology; D010539:Permeability; D019936:Probiotics / Q000494:pharmacology*; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D000097043:Interleukin-22
chemical_list: D018932:Chemokine CCL2; D062445:Claudin-1; D004041:Dietary Fats; D004731:Endotoxins; D005232:Fatty Acids, Volatile; D007328:Insulin; D016753:Interleukin-10; D007378:Interleukins; D062793:Occludin; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22; C491768:Ccl2 protein, mouse; C556062:IL10 protein, mouse; C568986:Ocln protein, mouse