title: Radix Ilicis Pubescentis total flavonoids ameliorates neuronal damage and reduces lesion extent in a mouse model of transient ischemic attack.
abstract: Flavonoids are a major component in the traditional Chinese medicine Radix Ilicis Pubescentis. Previous studies have shown that the administration of Radix Ilicis Pubescentis total flavonoids is protective in cerebral ischemia. However, to our knowledge, no studies have examined whether the total flavonoids extracted from Radix Ilicis Pubescentis prevent or ameliorate neuronal damage following transient ischemic attacks. Therefore, Radix Ilicis Pubescentis total flavonoids question and the potential underlying mechanisms. Thus, beginning 3 days before the induction of a mouse model of transient ischemic attack using tert-butyl hydroperoxide injections, mice were intragastrically administered 0.3, 0.15, or 0.075 g/kg of Radix Ilicis Pubescentis total flavonoids daily for 10 days. The results of spectrophotometric analyses demonstrated that Radix Ilicis Pubescentis total flavonoids enhanced oxygen free radical scavenging and reduced pathological alterations in the brain. Hematoxylin-eosin staining results showed that Radix Ilicis Pubescentis total flavonoids reduced hippocampal neuronal damage and cerebral vascular injury in this mouse model of transient ischemic attack. These results suggest that the antioxidant effects of Radix Ilicis Pubescentis total flavonoids alleviate the damage to brain tissue caused by transient ischemic attack.
authors.affiliation: College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Ming-San
authors.identifier: 0000-0003-2681-4291
authors.initials: MS
authors.lastname: Miao
authors.affiliation: College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Rui-Qi
authors.initials: RQ
authors.lastname: Li
authors.affiliation: College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Zhang
keywords: Radix Ilicis Pubescentis total flavonoids; malondialdehyde; nerve regeneration; neural regeneration; nitric oxide; nitric oxide synthetase; superoxide dismutase; tert-butyl hydroperoxide; transient ischemic attack