title: A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere.
abstract: Transcriptional inactivation of the budding yeast centromere has been a widely used tool in studies of chromosome segregation and aneuploidy. In haploid cells when an essential chromosome contains a single conditionally inactivated centromere (GAL-CEN), cell growth rate is slowed and segregation fidelity is reduced; but colony formation is nearly 100%. Pedigree analysis revealed that only 30% of the time both mother and daughter cell inherit the GAL-CEN chromosome. The reduced segregation capacity of the GAL-CEN chromosome is further compromised upon reduction of pericentric cohesin (mcm21∆), as reflected in a further diminishment of the Mif2 kinetochore protein at GAL-CEN. By redistributing cohesin from the nucleolus to the pericentromere (by deleting SIR2), there is increased presence of the kinetochore protein Mif2 at GAL-CEN and restoration of cell viability. These studies identify the ability of cohesin to promote chromosome segregation via kinetochore assembly, in a situation where the centromere has been severely compromised.
authors.affiliation: Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Tsabar
authors.affiliation: Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: Julian
authors.initials: J
authors.lastname: Haase
authors.affiliation: Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Harrison
authors.affiliation: Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: Chloe E
authors.initials: CE
authors.lastname: Snider
authors.affiliation: Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: Brittany
authors.identifier: http://orcid.org/0000-0003-2000-8301
authors.initials: B
authors.lastname: Eldridge
authors.affiliation: Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts, United States of America.
authors.forename: Lila
authors.initials: L
authors.lastname: Kaminsky
authors.affiliation: Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts, United States of America.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Hine
authors.affiliation: Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts, United States of America.
authors.forename: James E
authors.initials: JE
authors.lastname: Haber
authors.affiliation: Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
authors.forename: Kerry
authors.initials: K
authors.lastname: Bloom
mesh_terms: D000705:Anaphase / Q000235:genetics / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D002503:Centromere / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006238:Haploidy; D018386:Kinetochores / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics*; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C118344:MCM21 protein, S cerevisiae; C083768:MIF2 protein, S cerevisiae