title: Hierarchical Proteinosomes for Programmed Release of Multiple Components.
abstract: A facile route to hierarchically organized multicompartmentalized proteinosomes based on a recursive Pickering emulsion procedure using amphiphilic protein-polymer nanoconjugate building blocks is described. The number of incarcerated guest proteinosomes within a single host proteinosome is controlled, and enzymes and genetic polymers encapsulated within targeted subcompartments to produce chemically organized multi-tiered structures. Three types of spatiotemporal response-retarded concomitant release, synchronous release or hierarchical release of dextran and DNA-are demonstrated based on the sequential response of the host and guest membranes to attack by protease, or through variations in the positioning of disulfide-containing cross-links in either the host or guest proteinosomes integrated into the nested architectures. Overall, our studies provide a step towards the construction of hierarchically structured synthetic protocells with chemically and spatially integrated proto-organelles.
authors.affiliation: MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Liu
authors.affiliation: MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
authors.affiliation: MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China. xinhuang@hit.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK. s.mann@bristol.ac.uk.
authors.forename: Stephen
authors.initials: S
authors.lastname: Mann
mesh_terms: D058045:Artificial Cells / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage; D003692:Delayed-Action Preparations / Q000737:chemistry*; D003911:Dextrans / Q000008:administration & dosage; D004655:Emulsions / Q000737:chemistry*; D058726:Nanoconjugates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D003911:Dextrans; D004655:Emulsions; D058726:Nanoconjugates; D011108:Polymers; D011506:Proteins; D013501:Surface-Active Agents; D004247:DNA
keywords: hierarchical structures; microcapsules; nanoconjugates; proteinosomes; release