title: Bacteriocin from epidemic Listeria strains alters the host intestinal microbiota to favor infection.
abstract: Listeria monocytogenes is responsible for gastroenteritis in healthy individuals and for a severe invasive disease in immunocompromised patients. Among the three identified L. monocytogenes evolutionary lineages, lineage I strains are overrepresented in epidemic listeriosis outbreaks, but the mechanisms underlying the higher virulence potential of strains of this lineage remain elusive. Here, we demonstrate that Listeriolysin S (LLS), a virulence factor only present in a subset of lineage I strains, is a bacteriocin highly expressed in the intestine of orally infected mice that alters the host intestinal microbiota and promotes intestinal colonization by L. monocytogenes, as well as deeper organ infection. To our knowledge, these results therefore identify LLS as the first bacteriocin described in L. monocytogenes and associate modulation of host microbiota by L. monocytogenes epidemic strains to increased virulence.
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France;
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Quereda
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France; Cellule Pasteur, Université Paris Diderot, Sorbonne Paris Cité, Paris F-75015, France;
authors.forename: Olivier
authors.initials: O
authors.lastname: Dussurget
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France;
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Nahori
authors.affiliation: Bioinformatics and Biostatistics Hub - Centre de Bioinformatique, Biostatistique et Biologie Intégrative, Unité mixte de Service et Recherche 3756 Institut Pasteur- Centre National de la Recherche Scientifique, Paris F-75015, France;
authors.forename: Amine
authors.initials: A
authors.lastname: Ghozlane
authors.affiliation: Bioinformatics and Biostatistics Hub - Centre de Bioinformatique, Biostatistique et Biologie Intégrative, Unité mixte de Service et Recherche 3756 Institut Pasteur- Centre National de la Recherche Scientifique, Paris F-75015, France;
authors.forename: Stevenn
authors.initials: S
authors.lastname: Volant
authors.affiliation: Bioinformatics and Biostatistics Hub - Centre de Bioinformatique, Biostatistique et Biologie Intégrative, Unité mixte de Service et Recherche 3756 Institut Pasteur- Centre National de la Recherche Scientifique, Paris F-75015, France;
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Dillies
authors.affiliation: Biomics Pole, Centre d'Innovation et Recherche Technologique, Institut Pasteur, Paris F-75015, France;
authors.forename: Béatrice
authors.initials: B
authors.lastname: Regnault
authors.affiliation: Biomics Pole, Centre d'Innovation et Recherche Technologique, Institut Pasteur, Paris F-75015, France;
authors.forename: Sean
authors.initials: S
authors.lastname: Kennedy
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherché 1319 Micalis, Jouy-en-Josas F-78350, France; AgroParisTech, Unité Mixte de Recherché 1319 Micalis, Jouy-en-Josas F-78350, France.
authors.forename: Stanislas
authors.initials: S
authors.lastname: Mondot
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France;
authors.forename: Barbara
authors.initials: B
authors.lastname: Villoing
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France;
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
authors.affiliation: Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France; javier.pizarro-cerda@pasteur.fr.
authors.forename: Javier
authors.initials: J
authors.lastname: Pizarro-Cerda
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000378:metabolism*; D058872:Epidemics; D005260:Female; D000069196:Gastrointestinal Microbiome; D054884:Host-Pathogen Interactions; D006801:Humans; D008089:Listeria monocytogenes / Q000472:pathogenicity / Q000502:physiology*; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050296:Microbial Viability; D014774:Virulence
chemical_list: D001430:Bacteriocins
keywords: Listeria; bacteriocin; epidemic; infection; intestinal microbiota