title: Inhibition of the partial nitritation by roxithromycin and Cu(II).
abstract: To facilitate the application of partial nitritation (PN) - anaerobic ammonium oxidation process in nitrogen removal from livestock wastewater, the inhibition of roxithromycin (ROX) and Cu(II) on the PN sludge was examined using a respirometric method. The results showed that the IC50 of ROX and Cu(II) on PN sludge were 346 and 74.3mgL(-1), respectively. The relative specific respiration rate (SRR) of ammonia-oxidizing bacteria (AOB) decreased from 87.4% to 17.7% with the ROX concentration increased from 0 to 500mgL(-1). When the concentration of Cu(II) increased from 0 to 160mgL(-1), the SRRs of AOB and nitrite-oxidizing bacteria decreased by 85.5% and 11.2%, respectively. According to the isobole plots analysis, combined suppression by ROX and Cu(II) was synergistic. Fourier transform infrared spectroscopy analyses showed that ROX exposure altered the positions of CO bonds, and the intensity of the absorption peak at 2100cm(-1) changed under Cu(II) exposure.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Guo
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Shi
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Xu
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Yang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Huang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Man-Ling
authors.initials: ML
authors.lastname: Shi
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: jrczju@aliyun.com.
authors.forename: Ren-Cun
authors.initials: RC
authors.lastname: Jin
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D003300:Copper / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D020128:Inhibitory Concentration 50; D058751:Livestock; D008372:Manure; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism; D015575:Roxithromycin / Q000008:administration & dosage / Q000494:pharmacology*; D012722:Sewage / Q000382:microbiology*; D017550:Spectroscopy, Fourier Transform Infrared; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D062065:Wastewater
chemical_list: D064751:Ammonium Compounds; D000900:Anti-Bacterial Agents; D003300:Copper; D008372:Manure; D009584:Nitrogen; D015575:Roxithromycin; D012722:Sewage; D062065:Wastewater
keywords: Anammox; Cu(II); Inhibition; Partial nitritation; Roxithromycin