title: Effect of caffeic acid phenethyl ester on oxidant and anti-oxidant status of liver and serum in a rat model with acute methanol intoxication.
abstract: BACKGROUND
  Methanol toxicity is one of the major public health problems because it can cause severe morbidity and mortality. Methanol intoxication causes changes in the balance between the production of free radicals and antioxidant capacity.
  
  
  OBJECTIVE
  We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on the total oxidant status, total antioxidant status (TAS), and oxidative stress index (OSI) parameters of the liver and the serum in a rat model of acute methanol intoxication.
  
  
  METHODS
  Rats were treated with intraperitoneal (i.p.) Methotrexate (MTX) for 7 days. On the 8th day, i.p. Methanol was administered in the methanol, ethanol and CAPE groups. Four hours after methanol treatment, ethanol was injected i.p. in the ethanol group; CAPE (i.p.) in the CAPE group; serum physiologic i.p. in other groups. After 8 hours, rats were killed and the serum and the liver samples were obtained for biochemical analyses.
  
  
  RESULTS
  The OSI value was significantly higher in the methanol group compared to the ethanol and CAPE groups. Serum TAS levels of the methanol group were significantly different compared to the control group, but not compared to the MTX group. The amelioration of oxidative stress was greater in the CAPE group compared to the ethanol group but was not statistically significant.
  
  
  CONCLUSIONS
  This study demonstrates that CAPE treatment ameliorates oxidative stress in the serum and liver in a rat model of acute methanol intoxication.
authors.affiliation: Department of Physiology, Faculty of Medicine, Zirve University, Gaziantep, Turkey.
authors.forename: Ü C
authors.initials: ÜC
authors.lastname: Yazgan
authors.affiliation: Departments of Immunology, Faculty of Medicine, Dicle University, Diyarbakır, 21280, Turkey. drbilalelbey@gmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Elbey
authors.affiliation: Biochemistry, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Kuş
authors.affiliation: Medical Biology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Baykal
authors.affiliation: Anatomy, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Keskin
authors.affiliation: Family Medicine, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Yılmaz
authors.affiliation: Ophthalmology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Şahin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002109:Caffeic Acids / Q000494:pharmacology*; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D000432:Methanol / Q000506:poisoning*; D008727:Methotrexate / Q000494:pharmacology; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D016877:Oxidants; D000431:Ethanol; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol; D000432:Methanol; D008727:Methotrexate
keywords: CAPE; Methanol intoxication; Oxidant stress index; Total antioxidant status; Total oxidant status