title: Deconstructing brain-derived neurotrophic factor actions in adult brain circuits to bridge an existing informational gap in neuro-cell biology.
abstract: Brain-derived neurotrophic factor (BDNF) plays an important role in neurodevelopment, synaptic plasticity, learning and memory, and in preventing neurodegeneration. Despite decades of investigations into downstream signaling cascades and changes in cellular processes, the mechanisms of how BDNF reshapes circuits in vivo remain unclear. This informational gap partly arises from the fact that the bulk of studies into the molecular actions of BDNF have been performed in dissociated neuronal cultures, while the majority of studies on synaptic plasticity, learning and memory were performed in acute brain slices or in vivo. A recent study by Bowling-Bhattacharya et al., measured the proteomic changes in acute adult hippocampal slices following treatment and reported changes in proteins of neuronal and non-neuronal origin that may in concert modulate synaptic release and secretion in the slice. In this paper, we place these findings into the context of existing literature and discuss how they impact our understanding of how BDNF can reshape the brain.
authors.affiliation: Center for Neural Science, New York University, New York, NY, USA.
authors.forename: Heather
authors.identifier: 0000-0002-2137-4066
authors.initials: H
authors.lastname: Bowling
authors.affiliation: Center for Brain Development and Repair, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, India.
authors.forename: Aditi
authors.identifier: 0000-0002-6260-2897
authors.initials: A
authors.lastname: Bhattacharya
authors.affiliation: Center for Neural Science, New York University, New York, NY, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Klann
authors.affiliation: Departments of Cell Biology, Physiology, and Neuroscience and Psychiatry, Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY, USA.
authors.forename: Moses V
authors.initials: MV
authors.lastname: Chao
keywords: BDNF; BONCAT; BONLAC; SILAC; adult slice proteomics; hippocampus; neuroproteomics; protein synthesis