title: Olig1 expression pattern in neural cells during rat spinal cord development.
abstract: OBJECTIVE
  Our purpose was to systematically investigate the expression pattern and role of Olig1 in neural cells during rat spinal cord development.
  
  
  METHODS
  Spinal cord tissues were dissected from Sprague-Dawley rats at embryonic day 14.5 (E14.5) and E18.5, postnatal day 0 (P0), P3, P7, postnatal 2 weeks (P2W), P4W, and adults (more than 2 months after birth), respectively. The expression of Olig1 was determined by Western blot and immunostaining. To observe expression of Olig1 in different neural cell types, a double immunohistochemical staining was performed using antibodies against Olig1 with O4, β-tubulin, glial fibrillary acidic protein (GFAP), and myelin basic protein, respectively.
  
  
  RESULTS
  The expression of Olig1 protein shows a significant level change in rat spinal cord at different developmental time points. Starting with E14.5, the expression gradually increased and peaked at E18.5. Olig1 decreased gradually from P3 and reached its lowest level on P7. However, interestingly, the Olig1 expression increased again from P2W, until adulthood. Olig1 was coexpressed with O4-positive oligodendrocyte progenitor cells (OPCs) and β-tubulin-positive neurons at all time points during development. Olig1 was also coexpressed transiently with GFAP-positive astrocytes at only E14.5. Olig1 was localized in the cytoplasm of O4- and β-tubulin-positive cells during the period from E14.5 to adult.
  
  
  CONCLUSIONS
  The expression of Olig1 in OPCs and neurons at all time points during development and in astrocytes at E14.5 suggests that Olig1 may play an important role in the generation and maturation of specific neural cells during development of spinal cord. Our results contribute to understanding the mechanism underlying developmental regulation of neural cells by Olig1.
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China; Department of Histology and Embryology, Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China; Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
authors.forename: Hezuo
authors.initials: H
authors.lastname: Lü
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China; Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Hu
keywords: Olig1; development; neural cells; spinal cord