title: Development of a novel monoclonal antibody to human inducible co-stimulator ligand (ICOSL): Biological characteristics and application for enzyme-linked immunosorbent assay.
abstract: ICOSL (B7-H2, CD275), a co-stimulatory molecule of the B7 superfamily, functions as a positive signal in immune response. To investigate whether ICOSL could be released into sera and the possible biological function of soluble ICOS (sICOSL), we generated and characterized a functional anti-human ICOSL monoclonal antibody (mAb), 20B10, and developed a novel enzyme-linked immunosorbent assay (ELISA) based on two anti-human ICOSL antibodies with different epitope specificities. Using the ELISA system, we found that sICOSL in the serum of healthy donors increases in an age-dependent manner and that the matrix metalloproteinase inhibitor (MMPI) could suppress sICOSL production. Together, these data demonstrate that the existence of circulating sICOSL in human serum might play an important role in immunoregulation.
authors.affiliation: Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China; Department of Immunology, Medical College of Soochow University, Suzhou 215006, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Hu
authors.affiliation: Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Immunology, Medical College of Soochow University, Suzhou 215006, China.
authors.forename: Jingnan
authors.initials: J
authors.lastname: An
authors.affiliation: Department of Immunology, Medical College of Soochow University, Suzhou 215006, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: liucuiping1980@126.com.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Liu
authors.affiliation: Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China; Department of Immunology, Medical College of Soochow University, Suzhou 215006, China. Electronic address: xueguangzh@126.com.
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D006825:Hybridomas; D060945:Inducible T-Cell Co-Stimulator Ligand / Q000276:immunology / Q000378:metabolism*; D007223:Infant; D007231:Infant, Newborn; D008213:Lymphocyte Activation / Q000187:drug effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; C498514:ICOSLG protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: Antibody; ELISA; ICOSL