title: Comparison of in vitro-cultivation of human mesenchymal stroma/stem cells derived from bone marrow and umbilical cord.
abstract: Cell-mediated therapy is currently considered as a novel approach for many human diseases. Potential uses range from topic applications with the regeneration of confined tissue areas to systemic applications. Stem cells including mesenchymal stroma/stem cells (MSCs) represent a highly attractive option. Their potential to cure or alleviate human diseases is investigated in a number of clinical trials. A wide variety of methods has been established in the past years for isolation, cultivation and characterization of human MSCs as expansion is presently deemed a prerequisite for clinical application with high numbers of cells carrying reproducible properties. MSCs have been retrieved from various tissues and used in a multitude of settings whereby numerous experimental protocols are available for expansion of MSCs in vitro. Accordingly, different isolation, culture and upscaling techniques contribute to the heterogeneity of MSC characteristics and the, sometimes, controversial results. Therefore, this review discusses and summarizes certain experimental conditions for MSC in vitro culture focusing on adult bone marrow-derived and neonatal umbilical cord-derived MSCs in order to enhance our understanding for MSC tissue sources and to stratify different procedures. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Orthopaedic Surgery, OE 8893, Hannover Medical School, Hannover, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Hoffmann
authors.affiliation: Department of Orthopaedic Surgery (Annastift), OE 6270, Hannover Medical School, Hannover, Germany.
authors.forename: Thilo
authors.initials: T
authors.lastname: Floerkemeier
authors.affiliation: Biochemistry and Tumour Biology Laboratory, Department of Obstetrics and Gynecology, OE 6411, Hannover Medical School, Hannover, Germany.
authors.forename: Catharina
authors.initials: C
authors.lastname: Melzer
authors.affiliation: Biochemistry and Tumour Biology Laboratory, Department of Obstetrics and Gynecology, OE 6411, Hannover Medical School, Hannover, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Hass
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D009928:Organ Specificity; D014470:Umbilical Cord / Q000166:cytology / Q000378:metabolism
keywords: bulk isolation; characterization; expansion; heterogeneity; in vitro; minimal consensus criteria; prospective isolation