title: Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection.
abstract: Rice blast is a recurrent fungal disease, and resistance to fungal infection is a complex trait. Therefore, a comprehensive examination of rice transcriptome and its variation during fungal infection is necessary to understand the complex gene regulatory networks. In this study, adopting Next-Generation Sequencing we profiled the transcriptomes and microRNAomes of rice varieties, one susceptible and the other resistant to M. oryzae, at multiple time points during the fungal infection. Our results revealed a substantial variation in the plant transcriptome and microRNAome as well as change to rice innate immunity during fungal infection. A number of putative R gene candidates were identified from a perturbed rice transcriptome analysis. The expression of genes and non-coding RNA molecules changed in both fungal resistant and susceptible plants during M. oryzae invasion discovered distinct pathways triggered in the susceptible and resistant plants. In addition, a number of fungus genes in the susceptible and resistant plants were constantly expressed at different time points, suggesting that they were likely to be the potential AVR genes. Our results revealed large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection, which would help to develop more robust blast-resistant rice plants.
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, P. R. China.
authors.forename: Ze-Yuan
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Institute for Systems Biology, Jianghan University, Wuhan, Hubei 430056, China.|Department of Computer Science and Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Xia
authors.affiliation: Institute for Systems Biology, Jianghan University, Wuhan, Hubei 430056, China.|Department of Computer Science and Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, P. R. China.
authors.forename: Quan-Feng
authors.initials: QF
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, P. R. China.
authors.forename: Yu-Chan
authors.initials: YC
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, P. R. China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Zhang
authors.affiliation: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Cong-Ying
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Zhu
authors.affiliation: Institute for Systems Biology, Jianghan University, Wuhan, Hubei 430056, China.|Department of Computer Science and Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.|Department of Genetics, Washington University in St. Louis, St. Louis, MO, 63130, USA.
authors.forename: Weixiong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, P. R. China.
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D007113:Immunity, Innate; D035683:MicroRNAs / Q000032:analysis*; D012275:Oryza / Q000276:immunology*; D010935:Plant Diseases / Q000276:immunology*
chemical_list: D035683:MicroRNAs