title: Construction and myogenic differentiation of 3D myoblast tissues fabricated by fibronectin-gelatin nanofilm coating.
abstract: In this study, we used a recently developed approach of coating the cells with fibronectin-gelatin nanofilms to build 3D skeletal muscle tissue models. We constructed the microtissues from C2C12 myoblasts and subsequently differentiated them to form muscle-like tissue. The thickness of the constructs could be successfully controlled by altering the number of seeded cells. We were able to build up to ∼76 μm thick 3D constructs that formed multinucleated myotubes. We also found that Rho-kinase inhibitor Y27632 improved myotube formation in thick constructs. Our approach makes it possible to rapidly form 3D muscle tissues and is promising for the in vitro construction of physiologically relevant human skeletal muscle tissue models.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Varvara
authors.initials: V
authors.lastname: Gribova
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nishiguchi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Michiya
authors.initials: M
authors.lastname: Matsusaki
authors.affiliation: Grenoble Institute of Technology, CNRS UMR 5628, Laboratory of Materials and Physical Engineering, 3 Parvis L. Néel, 38016 Grenoble, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Boudou
authors.affiliation: Grenoble Institute of Technology, CNRS UMR 5628, Laboratory of Materials and Physical Engineering, 3 Parvis L. Néel, 38016 Grenoble, France. Electronic address: catherine.picart@grenoble-inp.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Picart
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: akashi@chem.eng.osaka-u.ac.jp.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Akashi
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D024510:Muscle Development / Q000502:physiology*; D032448:Myoblasts, Skeletal / Q000166:cytology* / Q000502:physiology*; D049329:Nanostructures / Q000737:chemistry; D009924:Organ Culture Techniques / Q000379:methods; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D005780:Gelatin
keywords: Extracellular matrix; Layer-by-layer; Myogenesis; Tissue engineering