title: Distinct expression of interferon-induced protein with tetratricopeptide repeats (IFIT) 1/2/3 and other antiviral genes between subsets of dendritic cells induced by dengue virus 2 infection.
abstract: Dengue virus (DENV) infection is an emerging public health hazard threatening inhabitants of the tropics and sub-tropics. Dendritic cells (DCs) are one of the major targets of DENV and the initiators of the innate immune response against the virus. However, current in vitro research on the DENV-DC interaction is hampered by the low availability of ex vivo DCs and donor variation. In the current study, we attempted to develop a novel in vitro DC model using immature DCs derived from the myeloid leukaemia cell line MUTZ-3 (IMDCs) to investigate the DENV-DC interaction. The IMDCs morphologically and phenotypically resembled human immature monocyte-derived dendritic cells (IMMoDCs). However, the permissiveness of IMDCs to DENV2 was lower than that of IMMoDCs. RT-PCR arrays showed that a group of type I interferon (IFN) -inducible genes, especially IFIT1, IFITM1, and IFI27, were significantly up-regulated in IMMoDCs but not in IMDCs after DENV2 infection. Further investigation revealed that IFIT genes were spontaneously expressed at both transcriptional and protein levels in the naive IMDCs but not in the naive IMMoDCs. It is possible that the poor permissiveness of IMDCs to DENV2 was a result of the high basal levels of IFIT proteins. We conclude that the IMDC model, although less permissive to DENV2, is a useful platform for studying the suppression mechanism of DENV2 and we expand the knowledge of cellular factors that modulate DENV2 infection in the human body.
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China.|HKU-Pasteur Research Pole, School of Public Health, The University of Hong Kong, Hong Kong, China.
authors.forename: Jingshu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China.|School of Health and Biomedical Sciences, Royal Melbourne Institute of Technology (RMIT) University, Melbourne, VIC, Australia.
authors.forename: Daniel Man-Yuen
authors.initials: DM
authors.lastname: Sze
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: Benjamin Yat-Ming
authors.initials: BY
authors.lastname: Yung
authors.affiliation: Molecular Regulation and Bioinformatics Laboratory, Department of Public Health and Parasitology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.|Graduate Institute of Biomedical Sciences, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan.
authors.forename: Petrus
authors.initials: P
authors.lastname: Tang
authors.affiliation: Graduate Institute of Biomedical Sciences, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan.|Department of Public Health and Parasitology, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan.
authors.forename: Wei-June
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong.
authors.forename: Kwok-Hung
authors.initials: KH
authors.lastname: Chan
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: Polly Hang-Mei
authors.initials: PH
authors.lastname: Leung
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D003713:Dendritic Cells / Q000502:physiology / Q000821:virology*; D003715:Dengue / Q000235:genetics / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007370:Interferon Type I / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000502:physiology / Q000821:virology*; D016601:RNA-Binding Proteins; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000943:Antigens, Differentiation; D002352:Carrier Proteins; C406887:IFI27 protein, human; C484290:IFIT1 protein, human; D007370:Interferon Type I; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; C062010:leu-13 antigen
keywords: MUTZ-3; dendritic cells; dengue virus