title: Donor/acceptor nanoparticle pair-based singlet oxygen channeling homogenous chemiluminescence immunoassay for quantitative determination of bisphenol A.
abstract: Bisphenol A (BPA) is widely used in consumer products such as plastic bottles and food containers. It has become a ubiquitous environmental contaminant and poses a serious risk to human health. A rapid, sensitive, and high-throughput method for detecting BPA is therefore desirable. Herein, a donor/acceptor nanoparticle pair-based singlet oxygen channeling chemiluminescence homogenous immunoassay is developed for the determination of BPA. The donor nanoparticles were modified with phthalocyanine as a photosensitizer and were then coated with streptavidin. The acceptor nanoparticles were doped with thioxene derivatives and Eu(III) as a chemiluminescence emitter and then coated with anti-BPA antibody. Under light irradiation, oxygen near the donor surface transforms to singlet oxygen (1O2), which migrates to the acceptor and reacts with it, generating luminescence. Because1O2has a very short lifetime, luminescence is generated only when the donor and acceptor are in close proximity. This occurs when they are brought together by the antigen/antibody and streptavidin/biotin reaction. Based on this singlet oxygen channeling mechanism, a competitive homogenous chemiluminescence immunoassay for BPA was developed on 384 microplates. The assay exhibited linear detection over the range 10-1000 ng/mL and a limit of detection of 2.9 ng/mL. The intra- and inter-assay precisions were both below 5.1 %. The average recoveries of three spiked samples in tap and river water samples were in the range 95.5-121.0 %, in agreement with values obtained using high-performance liquid chromatography. The homogeneous assay is rapid, low cost, sensitive, and allows high-throughput, so is well suited for screening large numbers of environmental samples. Graphical abstract Principle of the singlet oxygen channeling homogenous chemiluminescence competitive immunoassay based on nanoparticle pairs for determination of BPA.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China. zlx@rcees.ac.cn.
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China.
authors.forename: Fanglan
authors.initials: F
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China. lhguo@rcees.ac.cn.|Institute of Environment and Health, Jianghan University, Wuhan, Hubei, 430056, China. lhguo@rcees.ac.cn.
authors.forename: Liang-Hong
authors.initials: LH
authors.lastname: Guo
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001559:Benzhydryl Compounds / Q000032:analysis*; D001710:Biotin / Q000737:chemistry; D005063:Europium / Q000737:chemistry; D005618:Fresh Water / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D007211:Indoles / Q000737:chemistry; D054833:Isoindoles; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D017319:Photosensitizing Agents / Q000737:chemistry; D026082:Singlet Oxygen / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D000906:Antibodies; D001559:Benzhydryl Compounds; D001710:Biotin; D005063:Europium; D007211:Indoles; D054833:Isoindoles; D010100:Oxygen; D010636:Phenols; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D019809:Streptavidin; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C013647:phthalocyanine
keywords: Bisphenol A; Chemiluminescence; Homogenous immunoassay; Oxygen channeling