title: Emergence of Norovirus GII.17 Variants among Children with Acute Gastroenteritis in South Korea.
abstract: Of 1,050 fecal specimens collected from January 2013 to August 2015 from children with acute gastroenteritis, 149 (14.2%) were found to be positive for norovirus. Norovirus GII was the most predominant genogroup (98.65%; 147 of 149). The genotypes detected in this study were GI (2; 1.3%), GII.Pe-GII.4 (109; 73.1%), GII.P17-GII.17 (16; 10.7%), GII.P12-GII.3 (8; 5.4%), GII.P12-GII.12 (8; 5.4%), GII.P4-GII.4 (5; 3.4%), and the recombinant GII.Pe-GII.17 (1; 0.7%). Of these, the novel GII.17 strain was the second most predominant, and the number of affected children appeared to continuously increase over time (2013 [2; 4.4%], 2014 [4; 9.3%], and 2015 [10; 16.4%]). Phylogenetic analysis of the full genome and ORF1, ORF2, and ORF3 nucleotide sequences showed that GII.17 was grouped in cluster III with other strains isolated from 2013 to 2015 and had a different evolutionary history from strains collected in 1978 to 2002 and 2005 to 2009 formed clusters I and II. However, the phylogenetic trees also showed that cluster III was divided into subclusters IIIa (CAU-55 and CAU-85) and IIIb (Kawasaki 2014) (CAU-193, CAU-265, CAU-267, CAU-283, and CAU-289). Comparative analysis of the VP1 capsid protein using 15 complete amino acid sequences from noroviruses isolated from 1978 to 2015 showed 99 amino acid changes. These results could be helpful for epidemiological studies to understand circulating norovirus genotypes in population.
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, South Korea.
authors.forename: Hien
authors.initials: H
authors.lastname: Dang Thanh
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, South Korea.
authors.forename: Van Thai
authors.initials: VT
authors.lastname: Than
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, South Korea.
authors.forename: Tinh Huu
authors.initials: TH
authors.lastname: Nguyen
authors.affiliation: Department of Pediatrics, Chung-Ang University College of Medicine, Seoul 06974, South Korea.
authors.forename: Inseok
authors.initials: I
authors.lastname: Lim
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, South Korea.
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D000208:Acute Disease; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D005784:Gene Amplification; D016679:Genome, Viral / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D029322:Norovirus / Q000235:genetics*; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA