title: Hepatocyte Growth Factor Reduces Free Cholesterol-Mediated Lipotoxicity in Primary Hepatocytes by Countering Oxidative Stress.
abstract: Cholesterol overload in the liver has shown toxic effects by inducing the aggravation of nonalcoholic fatty liver disease to steatohepatitis and sensitizing to damage. Although the mechanism of damage is complex, it has been demonstrated that oxidative stress plays a prominent role in the process. In addition, we have proved that hepatocyte growth factor induces an antioxidant response in hepatic cells; in the present work we aimed to figure out the protective effect of this growth factor in hepatocytes overloaded with free cholesterol. Hepatocytes from mice fed with a high-cholesterol diet were treated or not with HGF, reactive oxygen species present in cholesterol overloaded hepatocytes significantly decreased, and this effect was particularly associated with the increase in glutathione and related enzymes, such as γ-gamma glutamyl cysteine synthetase, GSH peroxidase, and GSH-S-transferase. Our data clearly indicate that HGF displays an antioxidant response by inducing the glutathione-related protection system.
authors.affiliation: Posgrado en Biología Experimental, DCBS, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico.
authors.forename: Mayra
authors.initials: M
authors.lastname: Domínguez-Pérez
authors.affiliation: Posgrado en Biología Experimental, DCBS, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Unidad de Medicina Traslacional, Clínica y Fundación Médica Sur, 14050 México, DF, Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Nuño-Lámbarri
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Laboratorio de Sinovioanálisis, Instituto Nacional de Rehabilitación, 14389 México, DF, Mexico.
authors.forename: Denise
authors.initials: D
authors.lastname: Clavijo-Cornejo
authors.affiliation: Instituto Nacional de Geriatría, Secretaria de Salud, 10200 México, DF, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Luna-López
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PRODEP-SEP, 09340 México, DF, Mexico.
authors.forename: Verónica
authors.initials: V
authors.lastname: Souza
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PRODEP-SEP, 09340 México, DF, Mexico.
authors.forename: Leticia
authors.initials: L
authors.lastname: Bucio
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PRODEP-SEP, 09340 México, DF, Mexico.
authors.forename: Roxana U
authors.initials: RU
authors.lastname: Miranda
authors.affiliation: Red Fisiopatología de las Enfermedades Hepáticas, PRODEP-SEP, 09340 México, DF, Mexico; Unidad de Hígado "Dr. José E González", Hospital Universitario, UANL, 66450 Monterrey, NL, Mexico.
authors.forename: Linda
authors.initials: L
authors.lastname: Muñoz
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PRODEP-SEP, 09340 México, DF, Mexico.
authors.forename: Luis Enrique
authors.initials: LE
authors.lastname: Gomez-Quiroz
authors.affiliation: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 México, DF, Mexico.
authors.forename: Salvador
authors.initials: S
authors.lastname: Uribe-Carvajal
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PRODEP-SEP, 09340 México, DF, Mexico.
authors.forename: María Concepción
authors.initials: MC
authors.lastname: Gutiérrez-Ruiz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D002784:Cholesterol / Q000633:toxicity*; D004032:Diet; D004797:Enzyme-Linked Immunosorbent Assay; D005978:Glutathione / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000097:blood*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D019859:Proto-Oncogene Proteins c-met / Q000097:blood; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D017228:Hepatocyte Growth Factor; D002784:Cholesterol; D019859:Proto-Oncogene Proteins c-met; D005978:Glutathione