title: Influence of Lipid Composition, pH, and Temperature on Physicochemical Properties of Liposomes with Curcumin as Model Drug.
abstract: The physicochemical properties of large unilamellar vesicles (LUVs) were assessed with respect to lipid composition, pH, time, and temperature by monitoring their size, zeta potential, drug payload, and thermal behavior. A conventional thin film hydration technique was employed to prepare liposomes from soy phosphatidylcholine (SPC), dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG), and a 7:3 (M/M) mixture of DPPC+DPPG along with 30 mole% cholesterol in each combination. While the size of liposomes depended on lipid composition, pH and temperature, the zeta potential was found to be independent of the pH of the medium, although it varied with liposome type. Spherical morphology and bilayer were observed by electron microscopy. The phase transition temperature increased with decreasing pH. Membrane micro-viscosity showed the highest value for SPC, and membrane rigidity increased with increasing pH. The entrapment efficiency of liposomes with reference to curcumin was as follows: DPPC>DPPC+DPPG>DPPG>SPC. Sustained release of curcumin was observed for all liposomes. Curcumin-loaded liposomes exhibited substantial antibacterial activity against the gram-positive bacteria Bacillus amyloliquefaciens. Additional studies are needed to improve the understanding of the effect of formulation variables on the physicochemical stability of liposomes.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University.
authors.forename: Biplab
authors.initials: B
authors.lastname: Roy
authors.forename: Pritam
authors.initials: P
authors.lastname: Guha
authors.forename: Ravi
authors.initials: R
authors.lastname: Bhattarai
authors.forename: Prasant
authors.initials: P
authors.lastname: Nahak
authors.forename: Gourab
authors.initials: G
authors.lastname: Karmakar
authors.forename: Priyam
authors.initials: P
authors.lastname: Chettri
authors.forename: Amiya Kumar
authors.initials: AK
authors.lastname: Panda
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000069977:Bacillus amyloliquefaciens / Q000187:drug effects*; D002627:Chemistry, Physical; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D013499:Surface Properties; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008055:Lipids; D008081:Liposomes; D003474:Curcumin