title: Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay.
abstract: The identification of rapid, reliable, and highly reproducible biological assays that can be standardized and routinely used in preclinical tests constitutes a promising approach to reducing drug discovery costs and time. This unit details a tandem, rapid, and reliable cell viability method for preliminary screening of chemical compounds. This assay measures metabolic activity and cell mass in the same cell sample using a dual resazurin/sulforhodamine B assay, eliminating the variation associated with cell seeding and excessive manipulations in assays that test different cell samples across plates. The procedure also reduces the amount of cells, test compound, and reagents required, as well as the time expended in conventional tests, thus resulting in a more confident prediction of toxic thresholds for the tested compounds. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, Cantanhede, Portugal.|These authors should be considered co-first authors.
authors.forename: Filomena S G
authors.initials: FSG
authors.lastname: Silva
authors.affiliation: Kazan Federal University, Institute of Fundamental Medicine and Biology, Kazan, Russia.|These authors should be considered co-first authors.
authors.forename: Irina G
authors.initials: IG
authors.lastname: Starostina
authors.affiliation: Kazan Federal University, Institute of Fundamental Medicine and Biology, Kazan, Russia.
authors.forename: Vilena V
authors.initials: VV
authors.lastname: Ivanova
authors.affiliation: Kazan Federal University, Institute of Fundamental Medicine and Biology, Kazan, Russia.
authors.forename: Albert A
authors.initials: AA
authors.lastname: Rizvanov
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, Cantanhede, Portugal.
authors.forename: Paulo J
authors.initials: PJ
authors.lastname: Oliveira
authors.affiliation: Department of Life Sciences, School of Sciences and Technology, University of Coimbra, Coimbra, Portugal.
authors.forename: Susana P
authors.initials: SP
authors.lastname: Pereira
mesh_terms: D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D003124:Colorimetry; D004396:Coloring Agents / Q000737:chemistry*; D003584:Cytological Techniques; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D010078:Oxazines / Q000737:chemistry*; D015203:Reproducibility of Results; D012235:Rhodamines / Q000737:chemistry*; D014116:Toxicology / Q000379:methods*; D014966:Xanthenes / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D007202:Indicators and Reagents; D010078:Oxazines; D012235:Rhodamines; D014966:Xanthenes; C005843:resazurin; C022027:lissamine rhodamine B
keywords: high throughput; human skin fibroblasts; preclinical tests; toxicity evaluation