title: CD47 expression in cryopreserved equine cutaneous masses and normal skin.
abstract: We investigated CD47 expression in cryopreserved sections of equine cutaneous masses and normal skin. CD47 is a cell surface protein expressed on many cell types and overexpressed in some tumors. Interaction of CD47 and signal regulatory protein-alpha (SIRPα) inhibits phagocytosis by macrophages. Formalin-fixed tissues from horses prospectively enrolled in the study were used to establish a histologic diagnosis. Immunohistochemical assays were performed on cryopreserved tissues using anti-CD47 antibodies or IgG control antibodies. CD47 was not expressed on equine normal skin but positivity to CD47 was present in 13 of 24 (54%) masses. Immunotherapy with anti-CD47 antibodies for equine cutaneous tumors that express CD47 warrants further investigation.
authors.affiliation: Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA scaston@iastate.edu.
authors.forename: Stephanie S
authors.initials: SS
authors.lastname: Caston
authors.affiliation: Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Cooper
authors.affiliation: Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
authors.forename: Prashanth
authors.initials: P
authors.lastname: Chandramani-Shivalingappa
authors.affiliation: Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
authors.forename: Brett A
authors.initials: BA
authors.lastname: Sponseller
authors.affiliation: Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
authors.forename: Jesse M
authors.initials: JM
authors.lastname: Hostetter
authors.affiliation: Departments of Veterinary Clinical Sciences (Caston, Cooper), Iowa State University, Ames, IAVeterinary Microbiology and Preventive Medicine (Chandramani-Shivalingappa, Sponseller), Iowa State University, Ames, IAVeterinary Pathology (Hostetter), Iowa State University, Ames, IACollege of Veterinary Medicine, and Department of Statistics (Sun), Iowa State University, Ames, IA.
authors.forename: Yaxuan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000818:Animals; D051928:CD47 Antigen / Q000032:analysis*; D015925:Cryopreservation / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis* / Q000473:pathology; D006736:Horses; D011446:Prospective Studies; D012878:Skin Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*
chemical_list: D051928:CD47 Antigen
keywords: CD47; equine; immunohistochemistry; macrophage; neoplasia; skin; tumor