title: The synergy effect of daidzein and genistein isolated from Butea superba Roxb. on the reproductive system of male mice.
abstract: Butea superba Roxb. (BS) has been used in Thai men as an aphrodisiac, and prevent erectile dysfunction. Nevertheless, the active ingredients, dosages, have not been cleared. Hence, this study was to investigate the effect of compounds from the BS on the reproductive parameters of male mice. The results revealed that BS was extracted to afford biochanin A and genistein, which were first reported on BS, and daidzein. The mice were treated by daidzein and genistein alone and in combination. The results showed that the sperm number and motility, cholesterol and testosterone level of all isoflavones-treated groups were significantly higher than controls (p < 0.01). Obviously, daidzein plus genistein exhibited a synergistic effect, which is also the first report, and resulted in significantly displayed higher levels of these parameters compared to others. So, the synergistic activity of these isoflavones may be useful in improving libido, erectile capacity and assist infertility of poor spermatozoa in men.
authors.affiliation: a School of Pharmacology, Institute of Science , Suranaree University of Technology , Nakhon Ratchasima , Thailand.
authors.forename: Griangsak
authors.initials: G
authors.lastname: Eumkeb
authors.affiliation: b School of Chemistry, Institute of Science , Suranaree University of Technology , Nakhon Ratchasima , Thailand.
authors.forename: Sudarat
authors.initials: S
authors.lastname: Tanphonkrang
authors.affiliation: a School of Pharmacology, Institute of Science , Suranaree University of Technology , Nakhon Ratchasima , Thailand.
authors.forename: Kittipot
authors.initials: K
authors.lastname: Sirichaiwetchakoon
authors.affiliation: a School of Pharmacology, Institute of Science , Suranaree University of Technology , Nakhon Ratchasima , Thailand.
authors.forename: Tanaporn
authors.initials: T
authors.lastname: Hengpratom
authors.affiliation: a School of Pharmacology, Institute of Science , Suranaree University of Technology , Nakhon Ratchasima , Thailand.
authors.forename: Wanatkamon
authors.initials: W
authors.lastname: Naknarong
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D031290:Butea / Q000737:chemistry*; D002784:Cholesterol / Q000378:metabolism; D004357:Drug Synergism; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D005837:Genitalia, Male / Q000187:drug effects*; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D013739:Testosterone / Q000378:metabolism
chemical_list: D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D013739:Testosterone; C004742:daidzein; D002784:Cholesterol; D019833:Genistein
keywords: Butea superba Roxb; biochanin A; daidzein; genistein; sperm number; synergistic effect; testosterone