title: Electrochemical DNA biosensor based on poly(2,6-pyridinedicarboxylic acid) modified glassy carbon electrode for the determination of anticancer drug gemcitabine.
abstract: In this study, a simple methodology was used to develop a new electrochemical DNA biosensor based on poly(2,6-pyridinedicarboxylic acid) (P(PDCA)) modified glassy carbon electrode (GCE). This modified electrode was used to monitor for the electrochemical interaction between the dsDNA and gemcitabine (GEM) for the first time. A decrease in oxidation signals of guanine after the interaction of the dsDNA with the GEM was used as an indicator for the selective determination of the GEM via differential pulse voltammetry (DPV). The guanine oxidation peak currents were linearly proportional to the concentrations of the GEM in the range of 1-30mgL(‒1). Limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.276mgL(‒1) and 0.922mgL(‒1), respectively. The reproducibility, repeatability, and applicability of the analysis to pharmaceutical dosage forms and human serum samples were also examined. In addition to DPV method, UV-vis and viscosity measurements were utilized to propose the interaction mechanism between the GEM and the dsDNA. The novel DNA biosensor could serve for sensitive, accurate and rapid determination of the GEM.
authors.affiliation: Ankara University, Faculty of Science, Department of Chemistry, Ankara 06100, Turkey. Electronic address: gaydogdu@science.ankara.edu.tr.
authors.forename: Gözde Aydoğdu
authors.initials: GA
authors.lastname: Tığ
authors.affiliation: Dumlupınar University, Faculty of Science and Arts, Department of Chemistry, Kütahya 43100, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Zeybek
authors.affiliation: Ankara University, Faculty of Science, Department of Chemistry, Ankara 06100, Turkey.
authors.forename: Şule
authors.initials: Ş
authors.lastname: Pekyardımcı
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000032:analysis*; D015374:Biosensing Techniques; D002244:Carbon; D004247:DNA; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000032:analysis; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D010848:Picolinic Acids; D015203:Reproducibility of Results; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D010848:Picolinic Acids; D003841:Deoxycytidine; D002244:Carbon; D004247:DNA; C004860:dipicolinic acid; D000093542:Gemcitabine
keywords: Differential pulse voltammetry; Gemcitabine; Glassy carbon electrode; Polymer; dsDNA