title: Optimization study on the hydrogen peroxide pretreatment and production of bioethanol from seaweed Ulva prolifera biomass.
abstract: The seaweed Ulva prolifera, distributed in inter-tidal zones worldwide, contains a large percentage of cellulosic materials. The technical feasibility of using U. prolifera residue (UPR) obtained after extraction of polysaccharides as a renewable energy resource was investigated. An environment-friendly and economical pretreatment process was conducted using hydrogen peroxide. The hydrogen peroxide pretreatment improved the efficiency of enzymatic hydrolysis. The resulting yield of reducing sugar reached a maximum of 0.42g/g UPR under the optimal pretreatment condition (hydrogen peroxide 0.2%, 50°C, pH 4.0, 12h). The rate of conversion of reducing sugar in the concentrated hydrolysates to bioethanol reached 31.4% by Saccharomyces cerevisiae fermentation, which corresponds to 61.7% of the theoretical maximum yield. Compared with other reported traditional processes on Ulva biomass, the reducing sugar and bioethanol yield are substantially higher. Thus, hydrogen peroxide pretreatment is an effective enhancement of the process of bioethanol production from the seaweed U. prolifera.
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Jiefen
authors.initials: J
authors.lastname: Cui
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Gaoli
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Zhengkun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Huashi
authors.initials: H
authors.lastname: Guan
authors.affiliation: Biology Department, Jackson State University, Jackson, MS 39217, USA.
authors.forename: Hueymin
authors.initials: H
authors.lastname: Hwang
authors.affiliation: Biology Department, Jackson State University, Jackson, MS 39217, USA.
authors.forename: Winfred G
authors.initials: WG
authors.lastname: Aker
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China. Electronic address: pengwang@ouc.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D011134:Polysaccharides; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry* / Q000378:metabolism; D044445:Ulva / Q000737:chemistry* / Q000378:metabolism
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D011134:Polysaccharides; D000431:Ethanol; D006861:Hydrogen Peroxide; D002480:Cellulase
keywords: Bioethanol; Enzymatic hydrolysis; Hydrogen peroxide; Pretreatment; Ulva prolifera residue