title: Antioxidant responses in soybean and alfalfa plants grown in DDTs contaminated soils: Useful variables for selecting plants for soil phytoremediation?
abstract: Phytoremediation is a low-cost alternative technology based on the use of plants to remove pollutants from the environment. Persistent organic pollutants such as DDTs with a long half-life in soils are attractive candidates for remediation. This study aimed to determine the potential of antioxidant response use in the evaluation of plants' tolerance for selecting species in phytoremediation purposes. Alfalfa and soybean plants were grown in DDT contaminated soils. After 60days, growth, protein content, antioxidant capacity, GST activity, concentration of proteic and non-proteic thiol groups, chlorophyll content and carotenoid content were measured in plant tissues. Results showed no effect on alfalfa or soybean photosynthetic pigments but different responses in the protein content, antioxidant capacity, GST activity and thiol groups on roots, stems and leaves, indicating that DDTs affected both species. Soybean showed higher susceptibility than alfalfa plants due to the lower antioxidant capacity and GST activity in leaves, in spite of having the lowest DDT accumulation. This study provides new insights into the role of oxidative stress as an important component of the plant's response to DDT exposure.
authors.affiliation: Laboratorio de Ecotoxicología y Contaminación Ambiental, Instituto de Investigaciones, Marinas y Costeras (IIMyC), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP) - Consejo Nacional de Investigaciones, Científicas y Técnicas (CONICET), Dean Funes 3350, Mar del Plata 7600, Argentina. Electronic address: fmmitton@mdp.edu.ar.
authors.forename: Francesca M
authors.initials: FM
authors.lastname: Mitton
authors.affiliation: Universidade Federal de Rio Grande - FURG, Instituto de Ciências Biológicas (ICB), Rio Grande do Sul, Brazil.
authors.forename: Josencler L
authors.initials: JL
authors.lastname: Ribas Ferreira
authors.affiliation: Laboratorio de Ecotoxicología y Contaminación Ambiental, Instituto de Investigaciones, Marinas y Costeras (IIMyC), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP) - Consejo Nacional de Investigaciones, Científicas y Técnicas (CONICET), Dean Funes 3350, Mar del Plata 7600, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Gonzalez
authors.affiliation: Laboratorio de Ecotoxicología y Contaminación Ambiental, Instituto de Investigaciones, Marinas y Costeras (IIMyC), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP) - Consejo Nacional de Investigaciones, Científicas y Técnicas (CONICET), Dean Funes 3350, Mar del Plata 7600, Argentina. Electronic address: kmiglior@mdp.edu.ar.
authors.forename: Karina S B
authors.initials: KSB
authors.lastname: Miglioranza
authors.affiliation: Universidade Federal de Rio Grande - FURG, Instituto de Ciências Biológicas (ICB), Rio Grande do Sul, Brazil.
authors.forename: José M
authors.initials: JM
authors.lastname: Monserrat
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D003634:DDT / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D000455:Medicago sativa / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D012989:Soil Pollutants; D002734:Chlorophyll; D002338:Carotenoids; D003634:DDT; D005982:Glutathione Transferase
keywords: Alfalfa; DDTs; Oxidative stress; Phytoremediation; Soybean