title: Uncertainty quantification in flux balance analysis of spatially lumped and distributed models of neuron-astrocyte metabolism.
abstract: Identifying feasible steady state solutions of a brain energy metabolism model is an inverse problem that allows infinitely many solutions. The characterization of the non-uniqueness, or the uncertainty quantification of the flux balance analysis, is tantamount to identifying the degrees of freedom of the solution. The degrees of freedom of multi-compartment mathematical models for energy metabolism of a neuron-astrocyte complex may offer a key to understand the different ways in which the energetic needs of the brain are met. In this paper we study the uncertainty in the solution, using techniques of linear algebra to identify the degrees of freedom in a lumped model, and Markov chain Monte Carlo methods in its extension to a spatially distributed case. The interpretation of the degrees of freedom in metabolic terms, more specifically, glucose and oxygen partitioning, is then leveraged to derive constraints on the free parameters to guarantee that the model is energetically feasible. We demonstrate how the model can be used to estimate the stoichiometric energy needs of the cells as well as the household energy based on the measured oxidative cerebral metabolic rate of glucose and glutamate cycling. Moreover, our analysis shows that in the lumped model the net direction of lactate dehydrogenase (LDH) in the cells can be deduced from the glucose partitioning between the compartments. The extension of the lumped model to a spatially distributed multi-compartment setting that includes diffusion fluxes from capillary to tissue increases the number of degrees of freedom, requiring the use of statistical sampling techniques. The analysis of the distributed model reveals that some of the conclusions valid for the spatially lumped model, e.g., concerning the LDH activity and glucose partitioning, may no longer hold.
authors.affiliation: Department of Mathematics, Applied Mathematics and Statistics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA. dxc57@case.edu.
authors.forename: Daniela
authors.initials: D
authors.lastname: Calvetti
authors.affiliation: School of Mathematics, University of Minnesota, 206 Church St SE, Minneapolis, MN, 55455, USA.
authors.forename: Yougan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Mathematics, Applied Mathematics and Statistics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
authors.forename: Erkki
authors.initials: E
authors.lastname: Somersalo
mesh_terms: D001253:Astrocytes / Q000378:metabolism*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D008959:Models, Neurological; D009474:Neurons / Q000378:metabolism*; D035501:Uncertainty
chemical_list: D018698:Glutamic Acid; D005947:Glucose
keywords: Bayesian flux balance analysis; Brain energy metabolism; Distributed model; Glucose partitioning; Lactate shuttle