title: Aggregation of AcMNPV LEF-10 and Its Impact on Viral Late Gene Expression.
abstract: The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) late expression factor gene lef-10 has been identified to be required for viral late gene expression by transient expression assay. Our previous work has shown that the gene product LEF-10 can form very stable high-molecular-weight complexes, but the structure and function of the protein remain unknown. In this study, we demonstrated that LEF-10 was essential for the replication of AcMNPV, and its truncated fragment containing amino acid residues 1 to 48 were sufficient to support the virus survival. Our data also suggested that the LEF-10 could spontaneously aggregate to form punctate spots in virus infected Sf9 cells at low frequency, and the aggregation of the protein could be induced by LEF-10 over-expression. When the protein aggregated to form punctate spots, soluble LEF-10 proteins were depleted and this could result in the down-regulation of viral late gene expression.
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Nan
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Ou
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, P. R. China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D061987:Sf9 Cells; D014779:Virus Replication / Q000235:genetics
chemical_list: D019476:Insect Proteins