title: Indirect and competitive enzyme-linked immunosorbent assays for monitoring the humoral response against human VEGF.
abstract: CIGB-247, a VEGF-based vaccine, was studied in a clinical trial. This advance demands the refinement of the methodologies for assessment of vaccine immune responses. This study aimed to improve the performance of ELISAs for detecting IgG antibodies against human VEGF and the blocking activity of the serum to inhibit the VEGF/VEGFR2 interaction. The best experimental conditions were established through the evaluation of several blocking buffers, immobilization surfaces, and plate suppliers using human sera as test samples. As a result, two controlled ELISAs were used in testing of elicited immune response against VEGF in patients immunized with CIGB-247.
authors.affiliation: a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Javier
authors.initials: J
authors.lastname: Sánchez Ramírez
authors.affiliation: a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Yanelys
authors.initials: Y
authors.lastname: Morera Díaz
authors.affiliation: b Department of Bioinformatics , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Alexis
authors.initials: A
authors.lastname: Musacchio Lasa
authors.affiliation: a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Mónica
authors.initials: M
authors.lastname: Bequet-Romero
authors.affiliation: a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Yasmiana
authors.initials: Y
authors.lastname: Muñoz Pozo
authors.affiliation: a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Lincidio
authors.initials: L
authors.lastname: Pérez Sánchez
authors.affiliation: c Department of Clinical Research , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Francisco
authors.initials: F
authors.lastname: Hernández-Bernal
authors.affiliation: d Animal Facility , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Osmany
authors.initials: O
authors.lastname: Mendoza Fuentes
authors.affiliation: e Center of Medical and Surgical Research (CIMEQ) , Playa Siboney, Havana , Cuba.
authors.forename: Katty-Hind
authors.initials: KH
authors.lastname: Selman-Housein
authors.affiliation: a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Jorge Víctor
authors.initials: JV
authors.lastname: Gavilondo Cowley
authors.affiliation: a Department of Pharmaceuticals , Center for Genetic Engineering and Biotechnology (CIGB) , Playa Cubanacán, Havana , Cuba.
authors.forename: Marta
authors.initials: M
authors.lastname: Ayala Avila
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D019496:Cancer Vaccines / Q000276:immunology*; D003412:Cricetulus; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006041:Goats; D006801:Humans; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D011485:Protein Binding; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*; D042442:Vascular Endothelial Growth Factors / Q000097:blood / Q000276:immunology* / Q000378:metabolism*
chemical_list: D019496:Cancer Vaccines; D007074:Immunoglobulin G; D042442:Vascular Endothelial Growth Factors; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: CIGB-247; VEGF; VEGFR2; competitive ELISA; humoral response; indirect ELISA