title: Effects of Fatty Acids on the Interfacial and Solution Behavior of Mixed Lipidic Aggregates Called Solid Lipid Nanoparticles.
abstract: Mutual miscibility of soylecithin, tristearin, fatty acids (FAs), and curcumin was assessed by means of surface pressure-area isotherms at the air-solution interface in order to formulate modified solid lipid nanoparticles (SLN). Appearance of minima in the excess area (Aex) and changes in free energy of mixing (∆G(0)ex) were recorded for systems with 20 mole% FAs. Modified SLNs, promising as topical drug delivery systems, were formulated using the lipids in combination with curcumin, stabilized by an aqueous Tween 60 solution. Optimal formulations were assessed by judiciously varying the FA chain length and composition. Physicochemical properties of SLNs were studied such as the size, zeta potential (by dynamic light scattering), morphology (by freeze fracture transmission electron microscopy), and thermal behavior (by differential scanning calorimetry). The size and zeta potential of the formulations were in the range 300-500 nm and -10 to -20 mV, respectively. Absorption and emission spectroscopic analyses supported the dynamic light scattering and differential scanning calorimetry data and confirmed localization of curcumin to the palisade layer of SLNs. These nanoparticles showed a sustained release of incorporated curcumin. Curcumin-loaded SLNs were effective against a gram-positive bacterial species, Bacillus amyloliquefaciens. Our results on the physicochemical properties of curcumin-loaded SLNs, the sustained release, and on antibacterial activity suggest that SLNs are promising delivery agents for topical drugs.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University.
authors.forename: Gourab
authors.initials: G
authors.lastname: Karmakar
authors.forename: Prasant
authors.initials: P
authors.lastname: Nahak
authors.forename: Pritam
authors.initials: P
authors.lastname: Guha
authors.forename: Biplab
authors.initials: B
authors.lastname: Roy
authors.forename: Priyam
authors.initials: P
authors.lastname: Chettri
authors.forename: Manish
authors.initials: M
authors.lastname: Sapkota
authors.forename: Suraj
authors.initials: S
authors.lastname: Koirala
authors.forename: Takeshi
authors.initials: T
authors.lastname: Misono
authors.forename: Kanjiro
authors.initials: K
authors.lastname: Torigoe
authors.forename: Shilpi
authors.initials: S
authors.lastname: Ghosh
authors.forename: Amiya Kumar
authors.initials: AK
authors.lastname: Panda
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000069977:Bacillus amyloliquefaciens / Q000187:drug effects*; D002152:Calorimetry, Differential Scanning; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D012996:Solutions; D013816:Thermodynamics
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D005227:Fatty Acids; D008055:Lipids; D011136:Polysorbates; D012996:Solutions; D003474:Curcumin