title: Cycloartane triterpenes from Beesia calthaefolia and their anticomplement structure-activity relationship study.
abstract: Fifteen cycloartane triterpenes were isolated from Beesia calthaefolia and among them one was new cycloartane triterpenoid. The structure of new compound was determined by the application of spectroscopic analyses and chemical methods. The fifteen compounds were evaluated for their anticomplement activity by classic pathway. The structure-activity relationship analysis indicated that the configurations of 12-OH is preferable to be α than β, and 18-OH can decrease while 15-OH can increase the anticomplement activity, but saponin with both 15-OH and 18-OH lost most of its activity. The glycosyl moiety of most isolated cycloartane triterpenes is xylosyl. When xylosyl was substituted by glucosyl or galactosyl, their anticomplement activities were decreased or increased, respectively. Further structure-activity relationship (SAR) studies must be carried out to achieve general conclusions regarding the effect of further functionalizations on the anticomplement saponins.
authors.affiliation: a Department of Clinical Pharmacology , General Hospital of PLA , Beijing 100853 , China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Mu
authors.affiliation: a Department of Clinical Pharmacology , General Hospital of PLA , Beijing 100853 , China.
authors.forename: Jin-Yuan
authors.initials: JY
authors.lastname: Zhao
authors.affiliation: b Shanxi University of Traditional Chinese Medicine , Taiyuan 030024 , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: a Department of Clinical Pharmacology , General Hospital of PLA , Beijing 100853 , China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D003169:Complement Inactivator Proteins / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005960:Glucosides; D015394:Molecular Structure; D029626:Ranunculaceae / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: C000613493:(20S*, 24R*)-epoxy-9,19-cyclolanostane-3beta,15alpha,16beta,18,25-pentaol-3-O-beta-D-glucopyranoside; D003169:Complement Inactivator Proteins; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D012503:Saponins; D014315:Triterpenes; C012749:anticomplement; C085352:cycloartane
keywords: Beesia calthaefolia; anticomplement activity; cycloartane glycoside; structure–activity relationship