title: The migration and differentiation of hUC-MSCs(CXCR4/GFP) encapsulated in BDNF/chitosan scaffolds for brain tissue engineering.
abstract: We previously developed a biomaterial scaffold that could effectively provide seed cells to a lesion cavity resulting from traumatic brain injury. However, we subsequently found that few transplanted human umbilical cord mesenchymal stem cells (hUC-MSCs) are able to migrate from the scaffold to the lesion boundary. Stromal derived-cell factor-1α and its receptor chemokine (C-X-C motif) receptor (CXCR)4 are chemotactic factors that control cell migration and stem cell recruitment to target areas. Given the low expression level of CXCR4 on the hUC-MSC membrane, lentiviral vectors were used to generate hUC-MSCs stably expressing CXCR4 fused to green fluorescent protein (GFP) (hUC-MSCs(CXCR4/GFP)). We constructed a scaffold in which recombinant human brain-derived neurotrophic factor (BDNF) was linked to chitosan scaffolds with the crosslinking agent genipin (CGB scaffold). The scaffold containing hUC-MSCs(CXCR4/GFP) was transplanted into the lesion cavity of a rat brain, providing exogenous hUC-MSCs to both lesion boundary and cavity. These results demonstrate a novel strategy for inducing tissue regeneration after traumatic brain injury.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, People's Republic of China.
authors.forename: Chuanjun
authors.initials: C
authors.lastname: Huang
authors.forename: Longxiang
authors.initials: L
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.forename: Dekang
authors.initials: D
authors.lastname: Nie
authors.forename: Yinan
authors.initials: Y
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Zuo
authors.forename: Wei
authors.initials: W
authors.lastname: Huan
authors.forename: Jinlong
authors.initials: J
authors.lastname: Shi
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D000070642:Brain Injuries, Traumatic / Q000628:therapy; D019208:Brain-Derived Neurotrophic Factor / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D039823:Iridoids / Q000737:chemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C527276:CXCR4 protein, human; C486264:Cxcr4 protein, rat; D039823:Iridoids; D019718:Receptors, CXCR4; D049452:Green Fluorescent Proteins; D048271:Chitosan; C007834:genipin