title: Inhibitory effect of STAT3 gene combined with CDDP on growth of human Wilms tumour SK-NEP-1 cells.
abstract: To investigate the effects of signal transducer and activator of transcription 3 (STAT3) combined with cisplatin (CDDP) on the growth of human Wilms tumour (WT) SK-NEP-1 cell subcutaneous xenografts in nude mice and the possible mechanisms. Human WT SK-NEP-1 cells were subcutaneously transplanted to establish the BALB/c nude mice xenograft model. Mice were randomly divided into five groups: blank control group, adenovirus control group (NC group), STAT3 group, CDDP group and STAT3 plus CDDP group (combination group). Tumour volume and tumour weight were observed during the therapeutic process. The expression levels of STAT3, glucose regulatory protein 78 (GRP78) and BCL2-associated X protein (BAX) were evaluated by immunohistochemical analysis. Compared with the STAT3 group or CDDP group, the tumour weight and volume was significantly reduced in the combination group (P<0.05). No statistical significance was found in NC group compared with the blank control group (P > 0.05). Immunohistochemical analysis showed that STAT3, GRP78 and BAX protein levels in the combination group were significantly higher than those in STAT3 group and CDDP group (P<0.05). Exogenous STAT3 and CDDP may synergistically inhibit the xenograft tumour growth through up-regulation of BAX protein via GRP78.
authors.affiliation: Department of Laboratory Medicine, Wenling Maternal and Child Health Care Hospital, Wenling 317500, Zhejiang Province, China.
authors.forename: Junrong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Wenling Maternal and Child Health Care Hospital, Wenling 317500, Zhejiang Province, China.
authors.forename: Nina
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The Second People's Hospital of Wenling City (Cancer Hospital in Taizhou, Shanghai Tumor Hospital in Taizhou Branch), Wenling 317502, Zhejiang Province, China.
authors.forename: Haijiang
authors.initials: H
authors.lastname: Qu
authors.affiliation: Department of Oncology, The Second People's Hospital of Wenling City (Cancer Hospital in Taizhou, Shanghai Tumor Hospital in Taizhou Branch), Wenling 317502, Zhejiang Province, China.
authors.forename: Guangxian
authors.initials: G
authors.lastname: You
authors.affiliation: Department of Pediatrics, Wenling Maternal and Child Health Care Hospital, Wenling 317500, Zhejiang Province, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Pediatrics, Wenling Maternal and Child Health Care Hospital, Wenling 317500, Zhejiang Province, China.
authors.forename: Caie
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China zilang1023@163.com drfengpan@139.com.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Wenling Maternal and Child Health Care Hospital, Wenling 317500, Zhejiang Province, China zilang1023@163.com drfengpan@139.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage; D000091342:Endoplasmic Reticulum Chaperone BiP; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D050796:STAT3 Transcription Factor / Q000235:genetics*; D009396:Wilms Tumor / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000235:genetics*
chemical_list: C494826:BAX protein, human; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D051028:bcl-2-Associated X Protein; D002945:Cisplatin
keywords: BAX; STAT3; Wilms tumour (WT); cisplatin (CDDP); glucose regulatory protein 78 (GRP78)