title: In Vitro and in Vivo Evaluation of Silicate-Coated Polyetheretherketone Fabricated by Electron Beam Evaporation.
abstract: Intrinsic bioinertness severely hampers the application of polyetheretherketone (PEEK), although in the field of dentistry it is considered to be an ideal titanium substitute implanting material. In this study, a bioactive silicate coating was successfully introduced onto PEEK surface by using electron beam evaporation (EBE) technology to improve its bioactivity and osseointegration of PEEK. Through controlling the duration of EBE, the incorporated amounts of silicon (Si) could be exquisitely adjusted to obtain proper biofunctionality, as assessed by cell adhesion, proliferation, osteogenic gene expression, and protein detection. In vivo, the samples were then tested in a femur implantation model to assay osseointegration effects in ovariectomized (OVX) rats. Remarkable enhancement of adhesion, spreading, osteogenesis, and differentiation of bone marrow stem cells (rBMSCs-OVX) were noted on silicate-coated samples. In particular, the group that was processed for 5 min with EBE (EBE-5 min) showed the most improvements in ALP activity and osteogenic-related gene expression compared to the remaining groups. Better osseointegration of the group that was processed for 8 min with EBE (EBE-8 min) was observed in vivo, as indicated by micro-CT test, fluorescent labeling, and histological and histomorphometric analyses. Collectively, the outcomes of the above experiments demonstrate that the present work is a meaningful attempt to promote osseointegration under osteoporotic conditions with only Si element incorporated to PEEK surface by the application of EBE technique. To the best of our knowledge, this work is the first demonstration of tuning the surface properties of PEEK via the adoption of an EBE-fabricated silicate coating to address an osteoporotic problem both in vitro and in vivo.
authors.affiliation: Department of Prosthodontics, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine , 639 Zhizaoju Road, Shanghai 200011, China.|Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wen
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Prosthodontics, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine , 639 Zhizaoju Road, Shanghai 200011, China.|Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Prosthodontics, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine , 639 Zhizaoju Road, Shanghai 200011, China.|Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Lianyi
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Prosthodontics, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine , 639 Zhizaoju Road, Shanghai 200011, China.|Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Qianju
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Hongya
authors.initials: H
authors.lastname: Pan
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, China.
authors.forename: Xuanyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Prosthodontics, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine , 639 Zhizaoju Road, Shanghai 200011, China.|Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology , 639 Zhizaoju Road, Shanghai 200011, China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001577:Benzophenones; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D005260:Female; D005269:Femur / Q000166:cytology / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007659:Ketones / Q000138:chemical synthesis* / Q000494:pharmacology*; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000494:pharmacology*; D011108:Polymers; D051381:Rats; D017640:Silicates / Q000737:chemistry*; D013234:Stem Cells / Q000187:drug effects
chemical_list: D001577:Benzophenones; D007659:Ketones; D011108:Polymers; D017640:Silicates; C063834:polyetheretherketone; D011092:Polyethylene Glycols
keywords: PEEK; electron beam evaporation; osseointegration; ovariectomy; silicate; stem cells