title: Met-myoglobin formation, accumulation, degradation, and myoglobin oxygenation monitoring based on multiwavelength attenuance measurement in porcine meat.
abstract: We propose a simple, rapid, and nondestructive method to investigate formation, accumulation, and degradation of met-myoglobin (met-Mb) and myoglobin oxygenation from the interior of porcine meat. For the experiment, color photos and attenuance spectra of porcine meat (well-bled muscle, fat, and mixed) were collected daily to perform colorimetric analysis and to obtain the differences of attenuance between 578 and 567 nm (A578-A567) and between 615 and 630 nm (A630-A615), respectively. Oxy-, deoxy-, and met-myoglobin concentration changes over storage time were also calculated using Beer–Lamberts’ law with reflectance intensities at 557, 582, and 630 nm. The change of A578-A567 was well matched with the change of myoglobin oxygenation, and the change of A630-A615 corresponded well with the formation and degradation of met-Mb. In addition, attenuation differences, A578-A567 and A630-A615, were able to show the formation of met-Mb earlier than colorimetric analysis. Therefore, the attenuance differences between wavelengths can be indicators for estimating myoglobin oxygenation and met-Mb formation, accumulation, and degradation, which enable us to design a simple device to monitor myoglobin activities in porcine meat.
authors.affiliation: Gwangju Institute of Science and Technology, Department of Medical System Engineering, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of Korea.
authors.forename: Thien
authors.initials: T
authors.lastname: Nguyen
authors.affiliation: Hanoi University of Science and Technology, Department of Electronics Technology and Biomedical Engineering, 1 Dai Co Viet Road, Hanoi 10000, Vietnam.
authors.forename: Kien Nguyen
authors.initials: KN
authors.lastname: Phan
authors.affiliation: Chonnam National University Medical School, Department of Dermatology, 42 Jebong-ro, Dong-gu, Gwangju 61469, Republic of Korea.
authors.forename: Jee-Bum
authors.initials: JB
authors.lastname: Lee
authors.affiliation: Gwangju Institute of Science and Technology, Department of Medical System Engineering, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of Korea.
authors.forename: Jae Gwan
authors.initials: JG
authors.lastname: Kim
mesh_terms: D000818:Animals; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D008460:Meat; D009132:Muscles / Q000737:chemistry; D009211:Myoglobin / Q000032:analysis* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D013552:Swine
chemical_list: D009211:Myoglobin; D010100:Oxygen