title: Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects.
abstract: In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl β-d-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000μgg(-1) caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.
authors.affiliation: Erciyes University, Department of Crop and Animal Production, Kayseri, Turkey. Electronic address: azizogluugur@hotmail.com.
authors.forename: Ugur
authors.initials: U
authors.lastname: Azizoglu
authors.affiliation: Erciyes University, Department of Biology, Kayseri, Turkey.
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Ayvaz
authors.affiliation: Erciyes University, Department of Agricultural Biotechnology, Kayseri, Turkey.
authors.forename: Semih
authors.initials: S
authors.lastname: Yılmaz
authors.affiliation: Düzce University, Department of Field Crops, Düzce, Turkey.
authors.forename: Salih
authors.initials: S
authors.lastname: Karabörklü
authors.affiliation: Erciyes University, Department of Biology, Kayseri, Turkey.
authors.forename: Rıdvan
authors.initials: R
authors.lastname: Temizgul
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000502:physiology* / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000633:toxicity; D003001:Cloning, Molecular; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism / Q000633:toxicity; D015870:Gene Expression; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism / Q000633:toxicity; D007300:Insect Control / Q000379:methods; D007306:Insecticides; D007814:Larva; D009036:Moths / Q000187:drug effects
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bt SY49-1; Gene cloning; Insecticidal activity; Recombinant Cry1Ab