title: Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway.
abstract: Phytic acid (PA) biosynthesis pathway genes were reported from multiple crop species. PA accumulation was enhanced during grain filling and at that time, hormones like Abscisic acid (ABA) and Gibberellic acid (GA₃) interplay to control the process of seed development. Regulation of wheat PA pathway genes has not yet been reported in seeds. In an attempt to find the clues for the regulation by hormones, the promoter region of wheat PA pathway genes was analyzed for the presence of cis-elements. Multiple cis-elements of those known to be involved for ABA, GA₃, salicylic acid (SA), and cAMP sensing were identified in the promoters of PA pathway genes. Eight genes (TaIMP, TaITPK1-4, TaPLC1, TaIPK2 and TaIPK1) involved in the wheat PA biosynthesis pathway were selected for the expression studies. The temporal expression response was studied in seeds treated with ABA and GA₃ using quantitative real time PCR. Our results suggested that exogenous application of ABA induces few PA pathway genes in wheat grains. Comparison of expression profiles for PA pathway for GA₃ and ABA suggested the antagonistic regulation of certain genes. Additionally, to reveal stress responses of wheat PA pathway genes, expression was also studied in the presence of SA and cAMP. Results suggested SA specific differential expression of few genes, whereas, overall repression of genes was observed in cAMP treated samples. This study is an effort to understand the regulation of PA biosynthesis genes in wheat.
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. sipla@nabi.res.in.
authors.forename: Sipla
authors.initials: S
authors.lastname: Aggarwal
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. shukla@nabi.res.in.
authors.forename: Vishnu
authors.initials: V
authors.lastname: Shukla
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. kaushal@nabi.res.in.
authors.forename: Kaushal Kumar
authors.initials: KK
authors.lastname: Bhati
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. bedi.mandeep5@gmail.com.
authors.forename: Mandeep
authors.initials: M
authors.lastname: Kaur
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. shivanisharma.mtech2012@gmail.com.
authors.forename: Shivani
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. anuradha@nabi.res.in.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. shrikant@nabi.res.in.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Mantri
authors.affiliation: Department of Biotechnology, National Agri-Food Biotechnology Institute, C-127 Industrial Area, S.A.S-Nagar, Phase-8, Mohali, Punjab 160071, India. pandeyak@nabi.res.in.
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Pandey
keywords: ABA; GA3; gene regulation; phytic acid; seed development; wheat