title: The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis.
abstract: Flagella-mediated motility and chemotaxis towards nutrients are important characteristics of Vibrio fischeri that play a crucial role in the development of its symbiotic relationship with its Hawaiian squid host Euprymna scolopes. The V. fischeri chemoreceptor A (VfcA) mediates chemotaxis toward amino acids. The periplasmic sensory domain of VfcA has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. The crystals belonged to space group P1, with unit-cell parameters a = 39.9, b = 57.0, c = 117.0 Å, α = 88.9, β = 80.5, γ = 89.7°. A complete X-ray diffraction data set has been collected to 1.8 Å resolution using cryocooling conditions and synchrotron radiation.
authors.affiliation: Infection and Immunity Program, Monash Biomedical Discovery Institute and Department of Microbiology, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Abu Iftiaf Md
authors.initials: AI
authors.lastname: Salah Ud-Din
authors.affiliation: Infection and Immunity Program, Monash Biomedical Discovery Institute and Department of Microbiology, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Anna
authors.initials: A
authors.lastname: Roujeinikova
mesh_terms: D048908:Aliivibrio fischeri / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D019897:Periplasm / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins
keywords: bacterial chemotaxis; methyl-accepting protein; receptor; sensing domain