title: Magnetization Transfer MR Imaging to Monitor Muscle Tissue Formation during Myogenic in Vivo Differentiation of Muscle Precursor Cells.
abstract: Purpose To determine whether magnetization transfer (MT) magnetic resonance (MR) imaging may serve as a quantitative measure of the degree of fiber formation during differentiation of muscle precursor cells into engineered muscle tissue as a potential noninvasive monitoring tool in mice. Materials and Methods The study was approved by the local ethics committee (no. StV 01/2008) and the local Veterinary Office (license no. 99/2013). Human muscle progenitor cells (hMPCs) derived from rectus abdominis muscles were subcutaneously injected into CD-1 nude mice (CD-1 nude mice, Crl:CD1-Foxn1nu; Charles River Laboratories, Wilmington, Mass) for development of muscle tissue. The mice underwent MR imaging examinations at 4.7 T at days 1, 3, 7, 14, 21, and 28 after cell transplantation by using a gradient-echo sequence with an MT prepulse and systematic variation of the off-resonance frequency (50-37 500 Hz) at an amplitude of 800°. Direct saturation was estimated from a Bloch equation simulation. The MT ratio (MTR) was correlated to immunohistochemistry findings, Western blot results, and results of myography. Data were analyzed by using one-way or two-way analysis of variance with the Sidak or Tukey multiple comparisons test. Results In the reference skeletal muscle, highest MT was found for 2500 Hz off-resonance frequency with an MTR ± standard deviation of 57.5% ± 3.5. The developing muscle tissue exhibited increasing MT values during the 28 days of myogenic in vivo differentiation and did not reach the values of native skeletal muscle. Mean values of MTR (2500 Hz) for hMPCs were 27.6% ± 6.3 (day 1), 24.7% ± 8.7 (day 3), 28.2% ± 5.7 (day 7), 35.9% ± 5.0 (day 14), 37.0% ± 7.9 (day 21), and 39.9% ± 8.1 (day 28). The results from MT MR imaging correlated qualitatively well with muscle tissue expression of specific skeletal markers, as well as muscle contractility. Conclusion MT MR imaging may be used to noninvasively monitor the process of myogenic in vivo differentiation of hMPCs as a biomarker of the quantity and quality of muscle fiber formation.©RSNA, 2016 Online supplemental material is available for this article.
authors.affiliation: From the Institute of Diagnostic and Interventional Radiology (M.R., C.E., M.C.W., A.B.) and Department of Urology (M.R., D.H., S.S., D.E.), University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland; and Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland (M.R.).
authors.forename: Markus
authors.initials: M
authors.lastname: Rottmar
authors.affiliation: From the Institute of Diagnostic and Interventional Radiology (M.R., C.E., M.C.W., A.B.) and Department of Urology (M.R., D.H., S.S., D.E.), University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland; and Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland (M.R.).
authors.forename: Deana
authors.initials: D
authors.lastname: Haralampieva
authors.affiliation: From the Institute of Diagnostic and Interventional Radiology (M.R., C.E., M.C.W., A.B.) and Department of Urology (M.R., D.H., S.S., D.E.), University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland; and Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland (M.R.).
authors.forename: Souzan
authors.initials: S
authors.lastname: Salemi
authors.affiliation: From the Institute of Diagnostic and Interventional Radiology (M.R., C.E., M.C.W., A.B.) and Department of Urology (M.R., D.H., S.S., D.E.), University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland; and Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland (M.R.).
authors.forename: Christian
authors.initials: C
authors.lastname: Eberhardt
authors.affiliation: From the Institute of Diagnostic and Interventional Radiology (M.R., C.E., M.C.W., A.B.) and Department of Urology (M.R., D.H., S.S., D.E.), University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland; and Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland (M.R.).
authors.forename: Moritz C
authors.initials: MC
authors.lastname: Wurnig
authors.affiliation: From the Institute of Diagnostic and Interventional Radiology (M.R., C.E., M.C.W., A.B.) and Department of Urology (M.R., D.H., S.S., D.E.), University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland; and Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland (M.R.).
authors.forename: Andreas
authors.initials: A
authors.lastname: Boss
authors.affiliation: From the Institute of Diagnostic and Interventional Radiology (M.R., C.E., M.C.W., A.B.) and Department of Urology (M.R., D.H., S.S., D.E.), University Hospital Zurich, Rämistrasse 100, CH-8091 Zurich, Switzerland; and Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St Gallen, Switzerland (M.R.).
authors.forename: Daniel
authors.initials: D
authors.lastname: Eberli
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000166:cytology*
chemical_list: D015415:Biomarkers