title: Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion.
abstract: Mammalian actin-binding protein-1 (mAbp1) is an adaptor protein that binds actin and modulates scission during endocytosis. Recent studies suggest that mAbp1 impairs cell invasion; however, the mechanism for the inhibitory effects of mAbp1 remain unclear. We performed a yeast two-hybrid screen and identified the adaptor protein, FHL2, as a novel binding partner that interacts with the N-terminal actin depolymerizing factor homology domain (ADFH) domain of mAbp1. Here we report that depletion of mAbp1 or ectopic expression of the ADFH domain of mAbp1 increased Rho GTPase signaling and breast cancer cell invasion. Moreover, cell invasion induced by the ADFH domain of mAbp1 required the expression of FHL2. Taken together, our findings show that mAbp1 and FHL2 are novel binding partners that differentially regulate Rho GTPase signaling and MTLn3 breast cancer cell invasion.
authors.affiliation: Program in Cellular and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Lindsy R
authors.initials: LR
authors.lastname: Boateng
authors.affiliation: Departments of Medical Microbiology and Immunology and Pediatrics, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: David
authors.initials: D
authors.lastname: Bennin
authors.affiliation: Departments of Medical Microbiology and Immunology and Pediatrics, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Sofia
authors.initials: S
authors.lastname: De Oliveira
authors.affiliation: Departments of Medical Microbiology and Immunology and Pediatrics, University of Wisconsin, Madison, Wisconsin 53706. Electronic address: huttenlocher@wisc.edu.
authors.forename: Anna
authors.initials: A
authors.lastname: Huttenlocher
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D060850:LIM-Homeodomain Proteins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains
chemical_list: C117063:DBNL protein, human; C105987:FHL2 protein, human; D060850:LIM-Homeodomain Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D014157:Transcription Factors
keywords: FHL2; Rho (Rho GTPase); adaptor protein; cell invasion; cell migration; focal adhesion; mAbp1