title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination.
abstract: Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR.
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark.
authors.forename: Sonia
authors.initials: S
authors.lastname: Silva
authors.affiliation: Department of Biology, Masaryk University, Kamenice 5/A7, 62500 Brno, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Altmannova
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark.
authors.forename: Nadine
authors.initials: N
authors.lastname: Eckert-Boulet
authors.affiliation: Department of Biology, Masaryk University, Kamenice 5/A7, 62500 Brno, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Kolesar
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark.
authors.forename: Irene
authors.initials: I
authors.lastname: Gallina
authors.affiliation: Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Hang
authors.affiliation: Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Inn
authors.initials: I
authors.lastname: Chung
authors.affiliation: Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Milica
authors.initials: M
authors.lastname: Arneric
authors.affiliation: Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Building 223, 2800 Kgs. Lyngby, Denmark.
authors.forename: Line Due
authors.initials: LD
authors.lastname: Buron
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Building 223, 2800 Kgs. Lyngby, Denmark.
authors.forename: Uffe H
authors.initials: UH
authors.lastname: Mortensen
authors.affiliation: Department of Biology, Masaryk University, Kamenice 5/A7, 62500 Brno, Czech Republic; National Centre for Biomolecular Research, Masaryk University, Kamenice 5/A4, Brno 625 00, Czech Republic; International Clinical Research Center, Center for Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic.
authors.forename: Lumir
authors.initials: L
authors.lastname: Krejci
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark. Electronic address: mlisby@bio.ku.dk.
authors.forename: Michael
authors.initials: M
authors.lastname: Lisby
mesh_terms: D015825:Chromosomes, Fungal / Q000235:genetics; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D059765:Homologous Recombination; D008239:Lysine / Q000378:metabolism; D008938:Mitosis / Q000235:genetics*; D000072417:Protein Domains; D051721:Rad52 DNA Repair and Recombination Protein / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D058207:Sumoylation
chemical_list: D004268:DNA-Binding Proteins; C497197:RAD52 protein, S cerevisiae; C400213:RAD59 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins; D008239:Lysine
keywords: Homologous recombination; Rad51; Rad52; Rad59; SUMOylation; Srs2