title: Human cytomegalovirus infection of human adipose-derived stromal/stem cells restricts differentiation along the adipogenic lineage.
abstract: Human adipose-derived stromal/stem cells (ASCs) display potential to be used in regenerative stem cell therapies and as treatments for inflammatory and autoimmune disorders. Despite promising use of ASCs as therapeutics, little is known about their susceptibility to infectious agents. In this study, we demonstrate that ASCs are highly susceptible to human cytomegalovirus (HCMV) infection and permissive for replication leading to release of infectious virions. Additionally, many basic ASC functions are inhibited during HCMV infection, such as differentiation and immunomodulatory potential. To our knowledge this is the first study examining potential adverse effects of HCMV infection on ASC biology. Our results suggest, that an active HCMV infection during ASC therapy may result in a poor clinical outcome due to interference by the virus.
authors.affiliation: Department of Microbiology and Immunology, Tulane University School of Medicine , New Orleans, LA, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Zwezdaryk
authors.affiliation: Department of Microbiology and Immunology, Tulane University School of Medicine , New Orleans, LA, USA.
authors.forename: M B
authors.initials: MB
authors.lastname: Ferris
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine , New Orleans, LA, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Strong
authors.affiliation: Department of Microbiology and Immunology, Tulane University School of Medicine , New Orleans, LA, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Morris
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA; Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Bunnell
authors.affiliation: Department of Nutritional Sciences, Texas Tech University , Lubbock, TX, USA.
authors.forename: N V
authors.initials: NV
authors.lastname: Dhurandhar
authors.affiliation: Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA; Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA; Department of Surgery, Tulane University School of Medicine, New Orleans, LA, USA; LaCell LLC, New Orleans, LA, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Gimble
authors.affiliation: Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA; Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Sullivan
keywords: Adipogenesis; adipose stem cell; cytomegalovirus; herpesvirus