title: Enhancement of denitrifying phosphorus removal and microbial community of long-term operation in an anaerobic anoxic oxic-biological contact oxidation system.
abstract: A two-sludge system consisting of anaerobic anoxic oxic-biological contact oxidation (A(2)/O-BCO) was developed to treat domestic wastewater with a low carbon/nitrogen (COD/TN) ratio (around 3.21) by shortening sludge retention time (SRT) for phosphorus accumulating organisms (PAOs) in the A(2)/O reactor and prolonging SRT for nitrifiers in the BCO reactor. Specifically, the BCO reactor was composed of three stages in series (N1, N2 and N3), so that simultaneous nitrogen and phosphorus removals by denitrifying PAOs (DNPAOs) was achieved in the A(2)/O reactor with [Formula: see text] as the electron acceptor from the BCO reactor. Long term operational tests (600 days) were conducted with various operational parameters [e.g., hydraulic retention time (HRTs), nitrate recycling ratio (Rs), volume ratio (Vs)] to examine the denitrifying phosphorus removal performance. The system exhibited the highest removal of TN and [Formula: see text] at the HRTs of 8 h, Rs of 300% and Vs of 2:4:1. The optimal TN and [Formula: see text] removals were 80.30% and 96.61% at low COD/TN of 3.21. The species diversity and microbial community examined by the Illumina MiSeq method demonstrated the fact of two-sludge system, and the improved community structure by long-term optimization was prominent comparing with the seed sludge. Additionally, Accumulibacter and Dechloromonas were the dominant functional PAOs with 25.74% in the A(2)/O reactor, while nitrifiers (including Nitrosomonas and Nitrospira) were gradually enriched with 13.10%, 21.33%, and 31.10% in the three stages of the BCO reactor.
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address: pyz@bjut.edu.cn.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000032:analysis; D058440:Denitrification; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000032:analysis; D010084:Oxidation-Reduction; D010758:Phosphorus / Q000302:isolation & purification* / Q000378:metabolism*; D059027:Recycling; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D002244:Carbon; D009566:Nitrates; D009584:Nitrogen; D010758:Phosphorus; D012722:Sewage; D062065:Wastewater
keywords: Anaerobic anoxic oxic–biological contact oxidation; Denitrifying phosphorus removal; Illumina MiSeq; Microbial diversity; Optimization