title: INSECTICIDAL ACTIVITY OF PROTEIN EXTRACTS OBTAINED FROM BULBS OF CHILEAN AMARYLLIDACEAE AGAINST TRIALEURODES VAPORARIORUM WESTWOOD AND PSEUDOCOCCUS VIBURNI SIGNORET.
abstract: Entomotoxic proteins are produced by plants in defence against insect herbivory. Some carbohydrate-binding proteins exhibit strong insecticidal activity affecting the survival, growth, development and feeding behavior of phytophagous insects. The occurrence of entomotoxic lectins is well documented in the Amaryllidaceae, a plant family spread world-wide. In Chile, this family is represented by numerous species, many of which are also of high ornamental value. Protein extracts were obtained from bulbs of five different species of Chilean Amaryllidaceae. A dose-response assay was carried out with two important pests: the greenhouse whitefly Trialeurodes vaporariorum Westwood and the mealybug Pseudococcus viburni Signoret. The extracts were offered to insects in a liquid artificial diet for three days and the mortality was scored. The Phycella australis Ravenna extract caused the highest insecticidal activity (T. vaporariorum LC₅₀: 7200 µg/mL; P. viburni LC₅₀: 9500 µg/mL). Applied at 1000 µg/mL in the diet the P. australis extract did not repel feeding of these pests. A mannose-binding lectin isolated from the bulbs of P. australis proved to be moderately toxic for these pests (T. vaporariorum LC₅₀: 1127 µg/mL; P. viburni LC₅₀: 2320 µg/mL).
authors.forename: N
authors.initials: N
authors.lastname: Zapata
authors.forename: M
authors.initials: M
authors.lastname: Vargas
authors.forename: A
authors.initials: A
authors.lastname: Coronado
authors.forename: E J M
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: G
authors.initials: G
authors.lastname: Smagghe
mesh_terms: D000818:Animals; D002677:Chile; D004305:Dose-Response Relationship, Drug; D006430:Hemiptera; D007300:Insect Control; D007306:Insecticides; D019668:Liliaceae / Q000737:chemistry*; D010936:Plant Extracts; D018517:Plant Roots / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D007306:Insecticides; D010936:Plant Extracts