title: Increasing solubility of red bell pepper carotenoids by complexation with 2-hydroxypropyl-β-cyclodextrin.
abstract: Red bell pepper carotenoids were complexed with 2-hydroxypropyl-β-cyclodextrin (2-HPβCD) in different mass ratios (1:4, 1:6, 1:8 and 1:10) through ultrasonic homogenization in order to increase carotenoid solubility and their use as natural pigment in food. Inclusion complexes, red bell pepper extract and physical mixtures were analyzed by DSC, FT-IR, (1)H NMR and DLS. Solubility assay was performed to identify the effect of complexation on the solubility of carotenoids. From characterization assays, results showed that inclusion process occurred for all tested ratios. Results for water solubility assays demonstrated clear differences between solubility index of inclusion complexes (8.06±2.59-16.55±4.40mg/mL) and physical mixtures (3.53±1.44-7.3±1.88mg/mL), while carotenoid extract was no water soluble, as expected. These results indicated that molecular inclusion of carotenoids in 2-HPβCD was efficient to enhance their solubility in water, enabling application of red bell pepper carotenoid as natural pigment and/or bioactive substances in food.
authors.affiliation: Programa de Pós-Graduação em Ciências Aplicadas a Produtos para Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Doutor Mário Viana 523, Santa Rosa, Niterói, 24241-000, Brazil.
authors.forename: Nicolly
authors.initials: N
authors.lastname: de Lima Petito
authors.affiliation: Faculdade de Nutrição, Universidade Federal Fluminense, Rua Mário Santos Braga 30, Campus do Valonguinho, Centro, Niterói, 24020-140, Brazil.
authors.forename: Daiana
authors.initials: D
authors.lastname: da Silva Dias
authors.affiliation: Instituto Nacional de Tecnologia, Av. Venezuela 82, Saúde, Rio de Janeiro 20081-312, Brazil.
authors.forename: Valéria Gonçalves
authors.initials: VG
authors.lastname: Costa
authors.affiliation: Programa de Pós-Graduação em Ciências Aplicadas a Produtos para Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Doutor Mário Viana 523, Santa Rosa, Niterói, 24241-000, Brazil.
authors.forename: Deborah Quintanilha
authors.initials: DQ
authors.lastname: Falcão
authors.affiliation: Programa de Pós-Graduação em Ciências Aplicadas a Produtos para Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Doutor Mário Viana 523, Santa Rosa, Niterói, 24241-000, Brazil. Electronic address: katia_lima@id.uff.br.
authors.forename: Kátia Gome
authors.initials: KG
authors.lastname: de Lima Araujo
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002212:Capsicum / Q000737:chemistry*; D002338:Carotenoids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins
chemical_list: D010936:Plant Extracts; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002338:Carotenoids
keywords: 2-Hydroxypropyl-β-cyclodextrin; Carotenoids; Molecular inclusion; Red bell pepper