title: Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry.
abstract: Signal transduction across cell membranes often requires assembly of heteromeric receptors with defined stoichiometry. In this issue of Structure, Morales-Perez et al. (2016) present elegant methods for the expression of heteromeric nicotinic acetylcholine receptors with a defined α4β2 stoichiometry involving controlled baculovirus-mediated transduction and subunit counting by measurement of two fluorescent signals.
authors.affiliation: Cold Spring Harbor Laboratory, W. M. Keck Structural Biology Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA.
authors.forename: Tomas
authors.initials: T
authors.lastname: Malinauskas
authors.affiliation: Cold Spring Harbor Laboratory, W. M. Keck Structural Biology Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA. Electronic address: furukawa@cshl.edu.
authors.forename: Hiro
authors.initials: H
authors.lastname: Furukawa
mesh_terms: D011978:Receptors, Nicotinic / Q000737:chemistry*
chemical_list: D011978:Receptors, Nicotinic