title: In vivo imaging of dendritic pruning in dentate granule cells.
abstract: We longitudinally imaged the developing dendrites of adult-born mouse dentate granule cells (DGCs) in vivo and found that they underwent over-branching and pruning. Exposure to an enriched environment and constraint of dendritic growth by disrupting Wnt signaling led to increased branch addition and accelerated growth, which were, however, counteracted by earlier and more extensive pruning. Our results indicate that pruning is regulated in a homeostatic fashion to oppose excessive branching and promote a similar dendrite structure in DGCs.
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: J Tiago
authors.initials: JT
authors.lastname: Gonçalves
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Cooper W
authors.initials: CW
authors.lastname: Bloyd
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Shtrahman
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Johnston
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Simon T
authors.initials: ST
authors.lastname: Schafer
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Parylak
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Thanh
authors.initials: T
authors.lastname: Tran
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Chang
authors.affiliation: Laboratory of Genetics, Salk Institute for Biological Sciences, La Jolla, California, USA.
authors.forename: Fred H
authors.initials: FH
authors.lastname: Gage
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism; D003712:Dendrites / Q000502:physiology*; D005260:Female; D006624:Hippocampus / Q000166:cytology*; D006706:Homeostasis / Q000502:physiology; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D059906:Neuroimaging / Q000379:methods; D009473:Neuronal Plasticity / Q000502:physiology*