title: Ewing's Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue.
abstract: The molecular mechanism responsible for Ewing's Sarcoma (ES) remains largely unknown. MicroRNAs (miRNAs), a class of small non-coding RNAs able to regulate gene expression, are deregulated in tumors and may serve as a tool for diagnosis and prediction. However, the status of miRNAs in ES has not yet been thoroughly investigated. This study compared global miRNAs expression in paraffin-embedded tumor tissue samples from 20 ES patients, affected by primary untreated tumors, with miRNAs expressed in normal human mesenchymal stromal cells (MSCs) by microarray analysis. A miRTarBase database was used to identify the predicted target genes for differentially expressed miRNAs. The miRNAs microarray analysis revealed distinct patterns of miRNAs expression between ES samples and normal MSCs. 58 of the 954 analyzed miRNAs were significantly differentially expressed in ES samples compared to MSCs. Moreover, the qRT-PCR analysis carried out on three selected miRNAs showed that miR-181b, miR-1915 and miR-1275 were significantly aberrantly regulated, confirming the microarray results. Bio-database analysis identified BCL-2 as a bona fide target gene of the miR-21, miR-181a, miR-181b, miR-29a, miR-29b, miR-497, miR-195, miR-let-7a, miR-34a and miR-1915. Using paraffin-embedded tissues from ES patients, this study has identified several potential target miRNAs and one gene that might be considered a novel critical biomarker for ES pathogenesis.
authors.affiliation: Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. antonina.parafioriti@gpini.it.
authors.forename: Antonina
authors.initials: A
authors.lastname: Parafioriti
authors.affiliation: Dipartimento di Medicina, Sezione di Medicina Interna B, Università di Verona, Verona 37134, Italy. caterina.bason@univr.it.
authors.forename: Caterina
authors.initials: C
authors.lastname: Bason
authors.affiliation: Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. elisabetta.armiraglio@asst-pini-cto.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Armiraglio
authors.affiliation: Dipartimento di Sanità Pubblica e Medicina di Comunità, Sezione di Epidemiologia e Statistica Medica, Università di Verona, Verona 37134, Italy. lucia.calciano@univr.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Calciano
authors.affiliation: Unità Operativa Complessa (U.O.C.) Chirurgia Ortopedica Oncologica, Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. PrimoAndrea.Daolio@asst-pini-cto.it.
authors.forename: Primo Andrea
authors.initials: PA
authors.lastname: Daolio
authors.affiliation: Unità Operativa Complessa (U.O.C.) Immunoematologia-Medicina Trasfusionale e Laboratorio di Ematologia, Laboratorio di Ricerca "Cellule Staminali" Azienda Unità Sanitaria Locale (AUSL)-Ospedale Santo Spirito, Pescara 65125, Italy. martina.berardocco@gmail.com.
authors.forename: Martina
authors.initials: M
authors.lastname: Berardocco
authors.affiliation: Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. andrea.dibernardo@asst-pini-cto.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Di Bernardo
authors.affiliation: Facoltà di Medicina Veterinaria, Università di Teramo, Teramo 64100, Italy. acolosimo@unite.it.
authors.forename: Alessia
authors.initials: A
authors.lastname: Colosimo
authors.affiliation: Dipartimento di Oncologia Pediatrica, Fondazione-Istituto di Ricovero e Cura a Carattere Scientifico-(IRCCS) Istituto Nazionale dei Tumori, Milano 20133, Italy. Roberto.Luksch@istitutotumori.mi.it.
authors.forename: Roberto
authors.initials: R
authors.lastname: Luksch
authors.affiliation: Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. annacberardi@yahoo.it.|Unità Operativa Complessa (U.O.C.) Immunoematologia-Medicina Trasfusionale e Laboratorio di Ematologia, Laboratorio di Ricerca "Cellule Staminali" Azienda Unità Sanitaria Locale (AUSL)-Ospedale Santo Spirito, Pescara 65125, Italy. annacberardi@yahoo.it.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Berardi
mesh_terms: D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016612:Paraffin Embedding; D012512:Sarcoma, Ewing / Q000235:genetics / Q000473:pathology*; D059467:Transcriptome; D055815:Young Adult
chemical_list: D035683:MicroRNAs
keywords: Ewing’s Sarcoma; human mesenchymal stem cells; miRTarBase database; microRNAs