title: Enhancing pesticide degradation using indigenous microorganisms isolated under high pesticide load in bioremediation systems with vermicomposts.
abstract: In biobed bioremediation systems (BBSs) with vermicomposts exposed to a high load of pesticides, 6 bacteria and 4 fungus strains were isolated, identified, and investigated to enhance the removal of pesticides. Three different mixtures of BBSs composed of vermicomposts made from greenhouse (GM), olive-mill (OM) and winery (WM) wastes were contaminated, inoculated, and incubated for one month (GMI, OMI and WMI). The inoculums maintenance was evaluated by DGGE and Q-PCR. Pesticides were monitored by HPLC-DAD. The highest bacterial and fungal abundance was observed in WMI and OMI respectively. In WMI, the consortia improved the removal of tebuconazole, metalaxyl, and oxyfluorfen by 1.6-, 3.8-, and 7.7-fold, respectively. The dissipation of oxyfluorfen was also accelerated in OMI, with less than 30% remaining after 30d. One metabolite for metalaxyl and 4 for oxyfluorfen were identified by GC-MS. The isolates could be suitable to improve the efficiency of bioremediation systems.
authors.affiliation: Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Department of Environmental Protection, C/Profesor Albareda, 1, 18008 Granada, Spain.
authors.forename: Jean Manuel
authors.initials: JM
authors.lastname: Castillo Diaz
authors.affiliation: Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Department of Environmental Protection, C/Profesor Albareda, 1, 18008 Granada, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Delgado-Moreno
authors.affiliation: Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Scientific Instrumentation Service, C/Profesor Albareda, 1, 18008 Granada, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Núñez
authors.affiliation: Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Department of Environmental Protection, C/Profesor Albareda, 1, 18008 Granada, Spain.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Nogales
authors.affiliation: Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (EEZ-CSIC), Department of Environmental Protection, C/Profesor Albareda, 1, 18008 Granada, Spain. Electronic address: esperanza.romero@eez.csic.es.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Romero
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D005658:Fungi / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D010575:Pesticides / Q000378:metabolism*
chemical_list: D010575:Pesticides
keywords: Agro-industrial vermicomposts; Bacteria–fungi consortium; Enhanced pesticide bioremediation; Pesticide metabolites; Q-PCR analysis