title: Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues.
abstract: The efficient processing of both mouse and human tissues is a valuable technique for characterizing tissue-associated immune cells. Here, we describe the techniques used and optimised within our laboratory for the enrichment and identification of antigen-presenting cells across a number of mouse and human tissues.
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore.
authors.forename: Naomi
authors.initials: N
authors.lastname: McGovern
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlitzer
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Janela
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore. Florent_ginhoux@immunol.a-star.edu.sg.
authors.forename: Florent
authors.initials: F
authors.lastname: Ginhoux
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000166:cytology* / Q000276:immunology; D018949:Antigens, CD1 / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D020405:Dermis / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology; D051379:Mice
chemical_list: D018949:Antigens, CD1; D000954:Antigens, Surface
keywords: Antigen-presenting cells; Dendritic cells; Flow cytometry; Human tissue processing