title: Effect of Syzygium cumini and Bauhinia forficata aqueous-leaf extracts on oxidative and mitochondrial parameters in vitro.
abstract: Aqueous-leaf extract of Syzygium cumini and Bauhinia forficata are traditionally used in the treatment of diabetes and cancer, especially in South America, Africa, and Asia. In this study, we analyzed the effects of these extracts on oxidative and mitochondrial parameters in vitro, as well as their protective activities against toxic agents. Phytochemical screenings of the extracts were carried out by HPLC analysis. The in vitro antioxidant capacities were compared by DPPH radical scavenging and Fe(2+) chelating activities. Mitochondrial parameters observed were swelling, lipid peroxidation and dehydrogenase activity. The major chemical constituent of S. cumini was rutin. In B. forficata were predominant quercetin and gallic acid. S. cumini reduced DPPH radical more than B. forficata, and showed iron chelating activity at all tested concentrations, while B. forficata had not similar property. In mitochondria, high concentrations of B. forficata alone induced a decrease in mitochondrial dehydrogenase activity, but low concentrations of this extract prevented the effect induced by Fe(2+)+H2O2. This was also observed with high concentrations of S. cumini. Both extracts partially prevented the lipid peroxidation induced by Fe(2+)/citrate. S. cumini was effective against mitochondrial swelling induced by Ca(2+), while B. forficata alone induced swelling more than Ca(2+). This study suggests that leaf extract of S. cumini might represent a useful therapeutic for the treatment of diseases related with mitochondrial dysfunctions. On the other hand, the consumption of B. forficata should be avoided because mitochondrial damages were observed, and this possibly may pose risk to human health.
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Assis
authors.initials: A
authors.lastname: Ecker
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Francielli
authors.initials: F
authors.lastname: Araujo Vieira
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Alessandro
authors.initials: A
authors.lastname: de Souza Prestes
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Matheus
authors.initials: M
authors.lastname: Mulling Dos Santos
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Angelica
authors.initials: A
authors.lastname: Ramos
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Dias Ferreira
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Teixeira de Macedo
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Vargas Klimaczewski
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Lopes Seeger
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: João Batista
authors.initials: JB
authors.lastname: Teixeira da Rocha
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Nilda B
authors.initials: NB
authors.lastname: de Vargas Barbosa
keywords: Bauhinia forficate; Syzygium cumini; antioxidant properties; mitochondria