title: Improvement in lipids extraction processes for biodiesel production from wet microalgal pellets grown on diammonium phosphate and sodium bicarbonate combinations.
abstract: Biomass productivity and growth kinetics for microalgae grown on sodium bicarbonate and diammonium phosphate were investigated. Different carbon and nitrogen ratios have shown different growth rates and biomass productivity and C:N ratio 50:10 as mgL(-1) has shown the best production than all. For effective lipids extraction from biomass thermolysis and sonolysis were carried out from wet biomass. Sonolysis at 2.3W intensity for 5min has released 8.58mg at neutral pH. More quantity of lipids was extracted when extraction was made at pH 4 and 10 which resulted 9mg and 9.28mg lipids respectively. Thermal treatment at 100°C for 10min has released 12.82mg lipid at neutral pH. In the same thermolysis at pH 4 and 10 more quantity of lipids was extracted which were 15.16mg and 14.81mg respectively. Finally transesterified lipids were analyzed through GC-MS for FAME composition analysis.
authors.affiliation: Department of Environmental Sciences, COMSATS Institute of Information Technology, University Road, Tobe Camp, Postal Code: 22060, Abbottabad, Pakistan. Electronic address: hasnainshah111@gmail.com.
authors.forename: Syed Hasnain
authors.initials: SH
authors.lastname: Shah
authors.affiliation: Department of Environmental Sciences, COMSATS Institute of Information Technology, University Road, Tobe Camp, Postal Code: 22060, Abbottabad, Pakistan. Electronic address: iaraja@ciit.net.pk.
authors.forename: Iftikhar Ahmed
authors.initials: IA
authors.lastname: Raja
authors.affiliation: Department of Environmental Sciences, COMSATS Institute of Information Technology, University Road, Tobe Camp, Postal Code: 22060, Abbottabad, Pakistan. Electronic address: drqaisar@ciit.net.pk.
authors.forename: Qaisar
authors.initials: Q
authors.lastname: Mahmood
authors.affiliation: Department of Environmental Sciences, COMSATS Institute of Information Technology, University Road, Tobe Camp, Postal Code: 22060, Abbottabad, Pakistan. Electronic address: pervez@ciit.net.pk.
authors.forename: Arshid
authors.initials: A
authors.lastname: Pervez
mesh_terms: D056804:Biofuels / Q000032:analysis; D018533:Biomass; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D058086:Microalgae / Q000254:growth & development*; D010710:Phosphates / Q000737:chemistry; D017693:Sodium Bicarbonate / Q000737:chemistry
chemical_list: D056804:Biofuels; D008055:Lipids; D010710:Phosphates; C024788:ammonium phosphate; D017693:Sodium Bicarbonate
keywords: Fatty acid methyl ester; Microalgae; Sonolysis; Thermolysis; Wet lipids extraction procedure