title: Chloroplast RNA-Binding Protein RBD1 Promotes Chilling Tolerance through 23S rRNA Processing in Arabidopsis.
abstract: Plants have varying abilities to tolerate chilling (low but not freezing temperatures), and it is largely unknown how plants such as Arabidopsis thaliana achieve chilling tolerance. Here, we describe a genome-wide screen for genes important for chilling tolerance by their putative knockout mutants in Arabidopsis thaliana. Out of 11,000 T-DNA insertion mutant lines representing half of the genome, 54 lines associated with disruption of 49 genes had a drastic chilling sensitive phenotype. Sixteen of these genes encode proteins with chloroplast localization, suggesting a critical role of chloroplast function in chilling tolerance. Study of one of these proteins RBD1 with an RNA binding domain further reveals the importance of chloroplast translation in chilling tolerance. RBD1 is expressed in the green tissues and is localized in the chloroplast nucleoid. It binds directly to 23S rRNA and the binding is stronger under chilling than at normal growth temperatures. The rbd1 mutants are defective in generating mature 23S rRNAs and deficient in chloroplast protein synthesis especially under chilling conditions. Together, our study identifies RBD1 as a regulator of 23S rRNA processing and reveals the importance of chloroplast function especially protein translation in chilling tolerance.
authors.affiliation: College of Plant Science, Jilin University, Changchun, P.R. China.|School of Integrated Plant Science, Plant Biology Section, Cornell University, Ithaca, New York, United States of America.
authors.forename: Shuai
authors.identifier: 0000-0001-5326-8682
authors.initials: S
authors.lastname: Wang
authors.affiliation: Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China.|Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P.R. China.
authors.forename: Ge
authors.identifier: 0000-0002-4365-7112
authors.initials: G
authors.lastname: Bai
authors.affiliation: College of Plant Science, Jilin University, Changchun, P.R. China.|School of Integrated Plant Science, Plant Biology Section, Cornell University, Ithaca, New York, United States of America.
authors.forename: Shu
authors.identifier: 0000-0001-8731-1749
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Integrated Plant Science, Plant Biology Section, Cornell University, Ithaca, New York, United States of America.
authors.forename: Leiyun
authors.initials: L
authors.lastname: Yang
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P.R. China.
authors.forename: Fen
authors.initials: F
authors.lastname: Yang
authors.affiliation: School of Integrated Plant Science, Plant Biology Section, Cornell University, Ithaca, New York, United States of America.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P.R. China.|Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, United States of America.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.affiliation: School of Integrated Plant Science, Plant Biology Section, Cornell University, Ithaca, New York, United States of America.|State key laboratory of Crops Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hua
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D003080:Cold Temperature / Q000009:adverse effects; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D009154:Mutation; D010641:Phenotype; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C000619112:RBD1 protein, Arabidopsis; D012338:RNA, Ribosomal, 23S; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins