title: Peripheral trafficking of bone-marrow-derived stem cells in patients with different types of gastric neoplasms.
abstract: Recently, there has been a growing interest in the importance of stem cells (SCs) in the development/progression of gastric neoplasms. In this study, we performed a comprehensive analysis of different populations of bone-marrow-derived stem cells (BMSCs) in patients with various types of gastric malignancies, including gastric cancer, gastrointestinal stromal tumors (GISTs), neuroendocrine neoplasms (NENs), and lymphomas. We found significantly lower numbers of circulating Lin-/CD45 +/ CD133 + hematopoietic stem/progenitor cells (HSPCs), and intensified peripheral trafficking of both Lin-/CD45-/CXCR4+/CD34+/CD133+ very small embryonic/epiblast-like stem cells (VSELs) and CD105 + /STRO-1 +/ CD45- mesenchymal SCs (MSCs) in patients with gastric cancer, but not in those with other types of gastric neoplasms. No significant differences in the absolute numbers of circulating CD34 +/ KDR +/ CD31 +/ CD45- endothelial progenitor cells (EPCs) were observed between the groups. This abnormal balance in the peripheral trafficking of BMSCs in patients with gastric cancer was neither associated with clinical stage of the disease nor with systemic levels of stromal-derived factor-1 (SDF-1), as these were comparable to the values observed in control individuals. Interestingly, the absolute numbers of circulating BMSCs correlated with the concentrations of complement cascade-derived anaphylatoxins/molecules (mainly C5b-9/membrane attack complex-MAC) and sphingosine-1-phosphate (S1P). In summary, our translational study revealed that abnormal peripheral trafficking of BMSCs occurs in patients with gastric cancer, but not in those with other types of gastric neoplasms. Further, our findings indicate that highlighted complement cascade-derived molecules and S1P, but not SDF-1, are significant players associated with this phenomenon.
authors.affiliation: Department of Internal Medicine, University of Zielona Góra, Zielona Góra, Poland; Department of Gastroenterology, Pomeranian Medical University in Szczecin, Szczecin, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Błogowski
authors.affiliation: Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Kraków, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Zuba-Surma
authors.affiliation: Department of Medical Analytics, Pomeranian Medical University in Szczecin , Szczecin, Poland.
authors.forename: Daria
authors.initials: D
authors.lastname: Sałata
authors.affiliation: Department of Medical Analytics, Pomeranian Medical University in Szczecin , Szczecin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Budkowska
authors.affiliation: Department of Medical Analytics, Pomeranian Medical University in Szczecin , Szczecin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dołęgowska
authors.affiliation: Department of Gastroenterology, Pomeranian Medical University in Szczecin , Szczecin, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Starzyńska
keywords: Bone-marrow-derived stem cells; gastric cancer; gastrointestinal stromal tumors (GIST); neuroendocrine neoplasms; stem cells