title: Temporal proteomic profiling of Chlamydia trachomatis-infected HeLa-229 human cervical epithelial cells.
abstract: Chlamydia trachomatis is the leading causative agent of bacterial sexually transmitted infections worldwide which can lead to female pelvic inflammatory disease and infertility. A greater understanding of host response during chlamydial infection is essential to design intervention technique to reduce the increasing incidence rate of genital chlamydial infection. In this study, we investigated proteome changes in epithelial cells during C. trachomatis infection by using an isobaric tags for relative and absolute quantitation (iTRAQ) labeling technique coupled with a liquid chromatography-tandem mass spectrometry (LC-MS(3) ) analysis. C. trachomatis (serovar D, MOI 1)-infected HeLa-229 human cervical carcinoma epithelial cells (at 2, 4 and 8 h) showed profound modifications of proteome profile which involved 606 host proteins. MGST1, SUGP2 and ATXN10 were among the top in the list of the differentially upregulated protein. Through pathway analysis, we suggested the involvement of eukaryotic initiation factor 2 (eIF2) and mammalian target of rapamycin (mTOR) in host cells upon C. trachomatis infection. Network analysis underscored the participation of DNA repair mechanism during C. trachomatis infection. In summary, intense modifications of proteome profile in C. trachomatis-infected HeLa-229 cells indicate complex host-pathogen interactions at early phase of chlamydial infection.
authors.affiliation: Department of Medical Microbiology, Tropical Infectious Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Grace Min Yi
authors.initials: GM
authors.lastname: Tan
authors.affiliation: Department of Medical Microbiology, Tropical Infectious Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Hui Jing
authors.initials: HJ
authors.lastname: Lim
authors.affiliation: Department of Medical Microbiology, Tropical Infectious Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Tee Cian
authors.initials: TC
authors.lastname: Yeow
authors.affiliation: Department of Medical Microbiology, Tropical Infectious Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Elaheh
authors.initials: E
authors.lastname: Movahed
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Chung Yeng
authors.initials: CY
authors.lastname: Looi
authors.affiliation: Center of Excellence in Infection Genomics, South Texas Center For Emerging Infectious Diseases, University of Texas at San Antonio, Texas, USA.
authors.forename: Rishein
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Center of Excellence in Infection Genomics, South Texas Center For Emerging Infectious Diseases, University of Texas at San Antonio, Texas, USA.
authors.forename: Bernard P
authors.initials: BP
authors.lastname: Arulanandam
authors.affiliation: Department of Medical Microbiology, Tropical Infectious Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Sazaly
authors.initials: S
authors.lastname: Abu Bakar
authors.affiliation: Faculty of Medicine, SEGi University, Petaling Jaya, Malaysia.
authors.forename: Negar Shafiei
authors.initials: NS
authors.lastname: Sabet
authors.affiliation: Department of Medical Microbiology, Tropical Infectious Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Li-Yen
authors.initials: LY
authors.lastname: Chang
authors.affiliation: Department of Medical Microbiology, Tropical Infectious Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Won Fen
authors.initials: WF
authors.lastname: Wong
mesh_terms: D000067736:Ataxin-10 / Q000235:genetics / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000254:growth & development* / Q000472:pathogenicity; D002853:Chromatography, Liquid; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics; D040901:Proteomics / Q000379:methods; D000068103:Serine-Arginine Splicing Factors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013194:Staining and Labeling / Q000379:methods; D058570:TOR Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: C503889:ATXN10 protein, human; D000067736:Ataxin-10; D015852:Eukaryotic Initiation Factor-2; C000595781:SUGP2 protein, human; D000068103:Serine-Arginine Splicing Factors; D005982:Glutathione Transferase; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; C041116:leukotriene-C4 synthase
keywords: Cervical epithelial cells; Chlamydia trachomatis; Mass spectrometry; Proteome expression; Sexual transmitted infection