title: Expression of CGRP in embryonic mouse masseter muscle.
abstract: Neuropeptide calcitonin gene-related peptide (CGRP) is a mediator of inflammation and head pain that influences the functional vascular blood supply. The CGRP also regulate myoblast and acetylcholine receptors on neuromuscular junctions in development. However, little is known about its appearance and location during mouse masseter muscle (MM) development. We detected the mRNA abundance of CGRP, vascular genesis markers (Vascular endothelial growth factor A (VEGF-A), PECAM (CD31), lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1)) and embryonic and adult myosin heavy chain (MyHCs) (embryonic, IIa, IIb, and IIx) using real-time RT-PCR during development from the embryonic stage to after birth (E12.5, E14.5, E17.5, E18.5, P0, P1 and P5). We also endeavored to analyze the expression and localization of CGRP in situ hybridization in the developing mouse MM during development from the embryonic stage to after birth (E12.5, E14.5, E17.5, and P1). The antisense probe for CGRP was detected by in situ hybridization at E12.5, E14.5 E17.5 and then no longer detected after birth. The CGRP, CD31, embryonic MyHC abundance levels are highest at E17.5 (p<0.001) and they show a pattern similar to that of the other markers from E12.5 to P5. PCA analysis indicates a specific relation between CGRP and embryonic MyHC, CD31, and LYVE-1 in MM development. Cluster analyses identified the following distinct clusters for mRNA abundance in the MM: cluster 1, P5; cluster 2, E12.5, E14.5, E17.5, E18.5, P0, and P1. The positive correlation between CGRP and embryonic MyHC (Pearson's r>0.65; p<0.01) was analyzed. These data suggested that CGRP may have an influence on embryonic MyHC during mouse MM development. CGRP also affects the angiogenesis markers at embryonic stages.
authors.affiliation: Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Azuma
authors.affiliation: Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Miwa
authors.affiliation: Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, Japan. Electronic address: iwaoa1@tokyo.ndu.ac.jp.
authors.forename: Iwao
authors.initials: I
authors.lastname: Sato
mesh_terms: D042501:Angiogenic Proteins / Q000378:metabolism*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D008297:Male; D008406:Masseter Muscle / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D018995:Myosin Heavy Chains / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000502:physiology*
chemical_list: D042501:Angiogenic Proteins; D018995:Myosin Heavy Chains; D015740:Calcitonin Gene-Related Peptide
keywords: Angiogenesis; Development; Myosin heavy chain; Neuropeptide calcitonin gene-related peptide