title: Enhanced thermal stability of the thylakoid membranes from spruce. A comparison with selected angiosperms.
abstract: Recently, we have found that thermal stability of photosystem II (PSII) photochemistry in spruce needles is higher than in other plants (barley, beech) cultivated under the same temperatures. In this work, temperature dependences of various characteristics of PSII organization were studied in order to obtain complex information on the thermal stability of PSII function and organization in spruce. Temperature dependency of circular dichroism spectra revealed by about 6 °C higher thermal stability of macrodomain organization in spruce thylakoid membranes in comparison with Arabidopsis and barley ones; however, thermal disintegration of light-harvesting complex of PSII did not significantly differ among the species studied. These results thus indicate that thermal stability of PSII macro-organization in spruce thylakoid membranes is enhanced to a similar extent as thermal stability of PSII photochemistry. Clear-native polyacrylamide gel electrophoresis of preheated thylakoids demonstrated that among the separated pigment-protein complexes, only PSII supercomplexes (SCs) revealed considerably higher thermal stability in spruce thylakoids as compared to Arabidopsis and barley ones. Hence we suggest that higher thermal stability of PSII macro-organization of spruce is influenced by the maintenance of PSII SCs in the thylakoid membrane. In addition, we discuss possible effects of different PSII organizations and lipid compositions on the thermal stability of spruce thylakoid membranes.
authors.affiliation: Faculty of Science, Ostrava University, 30. dubna 22, 701 03, Ostrava 1, Czech Republic. vaclav.karlicky@osu.cz.|Global Change Research Institute CAS, Bělidla 986/4a, 603 00, Brno, Czech Republic. vaclav.karlicky@osu.cz.
authors.forename: Václav
authors.initials: V
authors.lastname: Karlický
authors.affiliation: Faculty of Science, Ostrava University, 30. dubna 22, 701 03, Ostrava 1, Czech Republic.|Global Change Research Institute CAS, Bělidla 986/4a, 603 00, Brno, Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Kurasová
authors.affiliation: Faculty of Science, Ostrava University, 30. dubna 22, 701 03, Ostrava 1, Czech Republic.
authors.forename: Božena
authors.initials: B
authors.lastname: Ptáčková
authors.affiliation: Global Change Research Institute CAS, Bělidla 986/4a, 603 00, Brno, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Večeřová
authors.affiliation: Global Change Research Institute CAS, Bělidla 986/4a, 603 00, Brno, Czech Republic.
authors.forename: Otmar
authors.initials: O
authors.lastname: Urban
authors.affiliation: Faculty of Science, Ostrava University, 30. dubna 22, 701 03, Ostrava 1, Czech Republic.|Global Change Research Institute CAS, Bělidla 986/4a, 603 00, Brno, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Špunda
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000502:physiology; D002734:Chlorophyll / Q000502:physiology; D000077194:Chlorophyll A; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D001467:Hordeum / Q000166:cytology / Q000502:physiology; D006358:Hot Temperature; D045332:Photosystem II Protein Complex / Q000502:physiology; D028222:Picea / Q000166:cytology* / Q000502:physiology; D020524:Thylakoids / Q000502:physiology*
chemical_list: D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D000077194:Chlorophyll A
keywords: Circular dichroism; Norway spruce; Photosystem II organization; Thermal stability; Thylakoid membrane