title: Alternative non-chromatographic method for alcohols determination in Clostridium acetobutylicum fermentations.
abstract: An economic, simple, quantitative, and non-chromatographic method for the determination of alcohols using microdiffusion principle has been adapted and validated for acetone-butanol-ethanol (ABE) fermentation samples. This method, based on alcohols oxidation using potassium dichromate in acid medium, and detection by spectrophotometry, was evaluated varying, both, temperature (35°C, 45°C, and 55°C) and reaction time (0 to 125min). With a sample analysis time of 90min at 45°C, a limit of detection (LOD), and a limit of quantification (LOQ) of 0.10, and 0.40g/L, respectively. The proposed method has been successfully applied to determine butanol and ethanol concentrations in ABE fermentation samples with the advantage that multiple samples can be analyzed simultaneously. The measurements obtained with the proposed method were in good agreement with those obtained with the Gas Chromatography Method (GCM). This proposed method is useful for routine analysis of alcohols and screening samples in laboratories and industries.
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Av. Instituto Politécnico Nacional # 2508, Col. San Pedro Zacatenco, CP 07360 , México D. F., Mexico.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Noriega-Medrano
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Av. Instituto Politécnico Nacional # 2508, Col. San Pedro Zacatenco, CP 07360 , México D. F., Mexico.
authors.forename: Jesús
authors.initials: J
authors.lastname: Vega-Estrada
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Av. Instituto Politécnico Nacional # 2508, Col. San Pedro Zacatenco, CP 07360 , México D. F., Mexico.
authors.forename: Jaime
authors.initials: J
authors.lastname: Ortega-López
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Av. Instituto Politécnico Nacional # 2508, Col. San Pedro Zacatenco, CP 07360 , México D. F., Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ruiz-Medrano
authors.affiliation: Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Lázaro Cárdenas, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomas, , C.P. 11340, Delegación Miguel Hidalgo, México, D.F., Mexico.
authors.forename: Eliseo
authors.initials: E
authors.lastname: Cristiani-Urbina
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Av. Instituto Politécnico Nacional # 2508, Col. San Pedro Zacatenco, CP 07360 , México D. F., Mexico. Electronic address: cmontes@cinvestav.mx.
authors.forename: Maria Del Carmen
authors.initials: Mdel C
authors.lastname: Montes-Horcasitas
mesh_terms: D056804:Biofuels / Q000032:analysis; D000440:Butanols / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D004058:Diffusion; D000431:Ethanol / Q000032:analysis*; D005285:Fermentation; D057230:Limit of Detection; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D056804:Biofuels; D000440:Butanols; D000431:Ethanol
keywords: ABE fermentation; Alcohols determination; Butanol determination; Clostridium acetobutylicum; Microdiffusion