title: The Reduction-insensitive Bonds of the MUC2 Mucin Are Isopeptide Bonds.
abstract: The main structural component of the mucus in the gastrointestinal tract is the MUC2 mucin. It forms large networks that in colon build the loose outer mucous layer that provides the habitat for the commensal flora and the inner mucous layer that protects the epithelial cells by being impenetrable to bacteria. The epithelial cells in mice lacking MUC2 are not adequately protected from bacteria, resulting in inflammation and the development of colon cancer as found in human ulcerative colitis. Correct processing of the MUC2 mucin is the basis for the building of these protective networks. During the biosynthesis of the MUC2 mucin, post-translational modifications are formed resulting in reduction-insensitive bonds between MUC2 monomers. By the use of γ-glutamyltranspeptidase and isopeptidase activity in leech saliva, we could show that the molecular nature of these reduction-insensitive bonds is isopeptide bonds formed between side chains of lysine and glutamine. Transglutaminase 2 has an affinity to the MUC2 CysD2 domain in the nanomolar range and can catalyze its cross-linking. By using mass spectrometry, we identified MUC2 residues involved in this cross-linking. This shows for the first time that transamidation is not only stabilizing the skin and the fibrin clot, but is also important for the correct intracellular processing of MUC2 to generate protective mucus.
authors.affiliation: From the Department of Medical Biochemistry, University of Gothenburg, SE-405 30 Gothenburg, Sweden.
authors.forename: Christian V
authors.initials: CV
authors.lastname: Recktenwald
authors.affiliation: From the Department of Medical Biochemistry, University of Gothenburg, SE-405 30 Gothenburg, Sweden gunnar.hansson@medkem.gu.se.
authors.forename: Gunnar C
authors.identifier: 0000-0002-1900-1869
authors.initials: GC
authors.lastname: Hansson
mesh_terms: D000818:Animals; D016466:CHO Cells; D003106:Colon / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D000072417:Protein Domains; D011499:Protein Processing, Post-Translational / Q000502:physiology*
chemical_list: C524625:MUC2 protein, human; D055262:Mucin-2
keywords: intestine; mass spectrometry (MS); mucin; mucus; transglutaminase