title: Transcriptional changes of mouse splenocyte organelle components following acute infection with Toxoplasma gondii.
abstract: Toxoplasmosis is a globally spread zoonosis. The pathogen Toxoplasma gondii can hijack cellular organelles of host for replication. Although a number of important cellular life events are controlled by cell organelles, very little is known of the transcriptional changes of host cellular organelles after infection with T. gondii. Herein, we performed RNA-sequencing (RNA-seq) and bioinformatics analyses to study the global organelle component changes. It was found that many transcripts of the mouse spleen cellular organelle components were altered by acute T. gondii infection with the RH strain (Type I). Most differentially expressed transcripts of mitochondrial components were downregulated, especially those involved in biosynthetic and metabolic processes. Moreover, mitochondria based apoptosis process was downregulated. In terms of cytoskeleton, most differentially expressed transcript of cytoskeleton components were also downregulated, including septin cytoskeleton, cytoskeleton organization, centrosome and myosin. For endolysosomal system, ion transporters were downregulated at mRNA level, whereas the cytolytic components were increased, such as granzymes, Rab27a and perforin1 (Prf1). The main transcripts of Golgi apparatus components involved in sialylation or vesicle-mediated transportation were downregulated, while immune related components were upregulated. For endoplasmic reticulum (ER), posttranslational modification, drug metabolism and material transportation related transcripts were downregulated. In addition, T. gondii antigen cross-presentation by MHC-I complex could be downregulated by the downregulation of CD76 and ubiquitination related transcripts. The present study, for the first time, described the transcriptional changes of the mouse spleen cellular organelles following acute T. gondii infection, which provides a foundation to study the interaction between T. gondii and host cells at the sub-cellular level.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: He
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
authors.forename: Fa-Cai
authors.initials: FC
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
authors.forename: Hui-Qun
authors.initials: HQ
authors.lastname: Song
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Animal Science, South China Agricultural University, Guangzhou, Guangdong Province 510642, PR China. Electronic address: mjxu2010@163.com.
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China. Electronic address: xingquanzhu1@hotmail.com.
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis; D019295:Computational Biology; D003599:Cytoskeleton / Q000378:metabolism; D015536:Down-Regulation; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000378:metabolism; D011992:Endosomes / Q000276:immunology / Q000378:metabolism; D004734:Energy Metabolism; D015870:Gene Expression; D006056:Golgi Apparatus / Q000378:metabolism; D008247:Lysosomes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D015388:Organelles / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D016053:RNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013154:Spleen / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D016053:RNA, Protozoan
keywords: Mouse; Organelles; RH strain (Type I); Spleen; Toxoplasma gondii; Transcriptional changes