title: A Disassembly Strategy for Imaging Endogenous Pyrophosphate in Mitochondria by Using an Fe(III) -salen Complex.
abstract: Inorganic pyrophosphate (PPi) is produced from nucleoside triphosphates in important biosynthetic reactions and is considered a diagnostic marker for various diseases, such as cancer, crystal deposition disease, and arthritis. Traditional methods for biological PPi detection rely on off-line analytics after sample destruction. Molecular probes for imaging this biologically important analyte with temporal and spatial control in living cells are currently in demand. Herein, we report an Fe(III) -salen complex as the first small reaction-based probe for endogenous mitochondrial PPi following a disassembly approach. Significantly, we successfully applied this complex for the detection of increased cellular PPi levels, and its performance was not affected by the presence of mitochondrial ATP in living cells.
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
authors.forename: Namita
authors.initials: N
authors.lastname: Kumari
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.|School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, 510275, P.R. China.
authors.forename: Huaiyi
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, 510275, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chao
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
authors.forename: Gilles
authors.initials: G
authors.lastname: Gasser
authors.affiliation: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland. felix.zelder@chem.uzh.ch.
authors.forename: Felix
authors.initials: F
authors.lastname: Zelder
mesh_terms: D000255:Adenosine Triphosphate; D000447:Aldehydes / Q000737:chemistry; D002463:Cell Membrane Permeability; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D011756:Diphosphates / Q000032:analysis*; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D028061:Phosphate Transport Proteins / Q000037:antagonists & inhibitors; D011339:Probenecid / Q000494:pharmacology
chemical_list: C498770:ANKH protein, human; D000447:Aldehydes; D056831:Coordination Complexes; D011756:Diphosphates; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D028061:Phosphate Transport Proteins; C013243:salicylaldehyde; C107241:diphosphoric acid; D000255:Adenosine Triphosphate; D011339:Probenecid
keywords: disassembly strategy; fluorescent probes; mitochondria; pyrophosphate; reaction-based probes