title: [Expression and Significance of Pim-3 Gene in Acute Myeloid Leukemia].
abstract: OBJECTIVE
  To investigate the role of Pim-3 abnomal expression in development of acute myeloid leukemia.
  
  
  METHODS
  Semi-quantitative RT-PCR was used to detect the expression of Pim-3 in bone marrow of 47 newly diagnosed and untreated patients with acute myeloid leukemia (AML) and 18 patients with AML after treatment with chemotherapy. At the same time, the bone marrow of 10 cases of non-hematologic malignancies was used as normal control. The difference of the Pim-3 gene expression in bone marrows among the 3 groups was also compared.
  
  
  RESULTS
  According to the RT-PCR detection results, the Pim-3 expression level in bone marrow of AML patients before chemotherapy were all significantly lower than those in patients with non-hematologic malignancies (P < 0.01). After chemotherapy, there were no significant differences of the Pim-3 expression level between the patients with acute myeloid leukemia and non-hematologic malignancies (P > 0.05), but the Pim-3 expression level was significantly lower in patients before chemotherapy as compared with that in patients post chemotherapy (P < 0.01). The comparison of Pim-3 expression before and after chemotherapy in remission group showed that Pim-3 expression levels before chemotherapy were all significantly lower than those after chemotherapy (P < 0.01), but there were no significant differences of Pim-3 expression levels between patients before and after chemotherapy in non-remission group (P > 0.05). The Pim-3 expression levels of non-remission patients after chemotherapy were all significantly lower than those of the remission patients after chemotherapy (P < 0.01).
  
  
  CONCLUSIONS
  Pim-3 gene is abnormally expressed in the AML patients before and after chemotherapy, and this gene may be involved in the genesis and development of acute myeloid leukemia.
authors.affiliation: Department of Hematology, The First People's Hospital of Zunyi, Zunyi 563003, Guizhou Province, China.
authors.forename: Zhen-Cang
authors.initials: ZC
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China. E-mail: thomeland@yeah.net.
authors.forename: Zu-Guo
authors.initials: ZG
authors.lastname: Tian
authors.affiliation: Department of Hematology, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D001853:Bone Marrow / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D011518:Proto-Oncogene Proteins; C499249:PIM3 protein, human; D017346:Protein Serine-Threonine Kinases