title: N-Linked Glycosylation Is Required for Vacuolar H+-ATPase (V-ATPase) a4 Subunit Stability, Assembly, and Cell Surface Expression.
abstract: The a subunit is the largest of 14 different subunits that make up the V-ATPase complex. In mammalian species this membrane protein has four paralogous isoforms, a1-a4. Clinically, a subunit isoforms are implicated in diverse diseases; however, little is known about their structure and function. The subunit has conserved, predicted N-glycosylation sites, and the a3 isoform has been directly shown to be N-glycosylated. Here we ask if human a4 (ATP6V0A4) is N-glycosylated at the predicted site, Asn489. We transfected HEK 293 cells, using the pCDNA3.1 expression-vector system, to express cDNA constructs of epitope-tagged human a4 subunit, with or without mutations to eliminate the putative glycosylation site. Glycosylation was characterized also by treatment with endoglycosidases; expression and localization were assessed by immunoblotting and immunofluorescence. Endoglycosidase-treated wild type (WT) a4 showed increased relative mobility on immunoblots, compared with untreated WT a4. This relative mobility was identical to that of unglycosylated mutant a4N489D, demonstrating that the a4 subunit is glycosylated. Cycloheximide pulse-chase experiments showed that the unglycosylated subunit degraded at a higher rate than the N-glycosylated form. Unglycosylated a4 was degraded mostly in the proteasomal pathway, but also, in part, through the lysosomal pathway. Immunofluorescence colocalization data showed that unglycosylated a4 was mostly retained in the ER, and that plasma membrane trafficking was defective. Co-immunoprecipitation studies suggested that a4N489Ddoes not assemble with the V-ATPase V1domain. Taken together, these data show that N-glycosylation plays a crucial role in a4 stability, and in V-ATPase assembly and trafficking to the plasma membrane. J. Cell. Biochem. 117: 2757-2768, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Faculty of Dentistry, Dental Research Institute, University of Toronto, Toronto, Ontario, Canada, M5G 1G6.
authors.forename: Sally
authors.initials: S
authors.lastname: Esmail
authors.affiliation: Faculty of Dentistry, Dental Research Institute, University of Toronto, Toronto, Ontario, Canada, M5G 1G6.
authors.forename: Yeqi
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Faculty of Dentistry, Dental Research Institute, University of Toronto, Toronto, Ontario, Canada, M5G 1G6.
authors.forename: Norbert
authors.initials: N
authors.lastname: Kartner
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada, M5S 1A8.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada, M5S 1A8.
authors.forename: Reinhart A F
authors.initials: RA
authors.lastname: Reithmeier
authors.affiliation: Faculty of Dentistry, Dental Research Institute, University of Toronto, Toronto, Ontario, Canada, M5G 1G6. m.manolson@dentistry.utoronto.ca.|Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada, M5S 1A8. m.manolson@dentistry.utoronto.ca.
authors.forename: Morris F
authors.initials: MF
authors.lastname: Manolson
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D055550:Protein Stability; D021122:Protein Subunits; D017386:Sequence Homology, Amino Acid; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; C490181:ATP6V0A4 protein, human; D025262:Vacuolar Proton-Translocating ATPases
keywords: ATPase; ER-associated degradation; N-glycosylation; membrane protein; protein degradation; proton pump; trafficking