title: MiR-595 targeting regulation of SOX7 expression promoted cell proliferation of human glioblastoma.
abstract: Increasing evidence indicated that dysregulation of microRNAs (miRNAs) were involved with human disease including cancer. Recently, miR-595 was reported as a tumor promoter in malignant mesothelioma. However, the underlying mechanism of miR-595 in human glioblastoma (GBM) cells have not been well elucidated. Therefore, in this study, we investigated the biological functions and molecular mechanisms of miR-595 in human GBM. MiR-595 expression was significantly upregulated in GBM tissues and cells. We modified miR-595 levels in GBM cells and investigated their effects on the cell proliferation by MTT, colony formation and anchorage-independent growth assays. We found that miR-595 significantly increased GBM cell proliferation. Bioinformatic analysis predicted that miR-595 may target the 3'-UTR of SOX7and suppressed its translation, and further confirmed by luciferase assay. In sum, these observations together indicated that miR-595 played a critical role in carcinogenesis by suppression of SOX7, and may serve as a therapeutic target for the treatment of GBM.
authors.affiliation: Clinical laboratory, Liaocheng People's Hospital, Liaocheng, Shandong 252000, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, PR China.
authors.forename: Shubao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Clinical laboratory, Liaocheng People's Hospital, Liaocheng, Shandong 252000, PR China. Electronic address: shaojunsun2011@163.com.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, PR China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, PR China.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D055760:SOXF Transcription Factors / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C484456:SOX7 protein, human; D055760:SOXF Transcription Factors; C000609695:microRNA595 microRNA, human
keywords: Cell proliferation; Glioblastoma; SOX7; miR-595