title: Proteomic analyses bring new insights into the effect of a dark stress on lipid biosynthesis in Phaeodactylum tricornutum.
abstract: Microalgae biosynthesize high amount of lipids and show high potential for renewable biodiesel production. However, the production cost of microalgae-derived biodiesel hampers large-scale biodiesel commercialization and new strategies for increasing lipid production efficiency from algae are urgently needed. Here we submitted the marine algae Phaeodactylum tricornutum to a 4-day dark stress, a condition increasing by 2.3-fold the total lipid cell quotas, and studied the cellular mechanisms leading to lipid accumulation using a combination of physiological, proteomic (iTRAQ) and genomic (qRT-PCR) approaches. Our results show that the expression of proteins in the biochemical pathways of glycolysis and the synthesis of fatty acids were induced in the dark, potentially using excess carbon and nitrogen produced from protein breakdown. Treatment of algae in the dark, which increased algal lipid cell quotas at low cost, combined with optimal growth treatment could help optimizing biodiesel production.
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.|Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Song
authors.affiliation: Quebec-Ocean and Takuvik Joint International Research Unit, Université Laval, Québec, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Lavoie
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.
authors.forename: Hanqi
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Fu
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou 310032, P. R. of China.|Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Chinese Academy of Sciences, Urumqi 830011, P. R. of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qian
mesh_terms: D020418:Algal Proteins / Q000096:biosynthesis / Q000235:genetics; D059001:Aquatic Organisms; D056804:Biofuels; D002244:Carbon / Q000378:metabolism; D003624:Darkness; D017377:Diatoms / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005227:Fatty Acids / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D023281:Genomics; D006019:Glycolysis / Q000235:genetics / Q000528:radiation effects; D050356:Lipid Metabolism / Q000235:genetics / Q000528:radiation effects*; D050155:Lipogenesis / Q000235:genetics / Q000528:radiation effects*; D058086:Microalgae / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D058977:Molecular Sequence Annotation; D009584:Nitrogen / Q000378:metabolism; D017440:Photoperiod; D040901:Proteomics; D013312:Stress, Physiological
chemical_list: D020418:Algal Proteins; D056804:Biofuels; D005227:Fatty Acids; D002244:Carbon; D009584:Nitrogen