title: Analysis of Dendritic Cell Function Using Clec9A-DTR Transgenic Mice.
abstract: The Clec9A-diphtheria toxin receptor (DTR) transgenic mouse strain provides a robust animal model to study the function of lymphoid organ-resident CD8(+) dendritic cells (DCs) and nonlymphoid organ-specific CD103(+) DCs in infectioous diseases and inflammation. Here we describe some basic protocols for CD8(+)/CD103(+) DC isolation, for their in vivo depletion, and for their characterization by multi-color flow cytometry analysis. As an example for in vivo functional characterization of this DC subset, we present here the experimental cerebral malaria model. Furthermore, we illustrate advantages and pitfalls of the Clec9A-DTR system.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
authors.forename: Piotr
authors.initials: P
authors.lastname: Tetlak
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore. ruedl@ntu.edu.sg.
authors.forename: Christiane
authors.initials: C
authors.lastname: Ruedl
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D066257:Heparin-binding EGF-like Growth Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism; D016779:Malaria, Cerebral / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
chemical_list: D015703:Antigens, CD; D016827:CD8 Antigens; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D039001:Integrin alpha Chains; D037181:Lectins, C-Type; C091367:alpha E integrins
keywords: Clec9A/DNGR-1; Cross-priming; Dendritic cell (DC) subsets; Diphtheria toxin receptor (DTR) transgenic mice; Interferon-γ experimental cerebral malaria (ΕCM)