title: The platelet derived growth factor-B polymorphism is associated with risk of severe fever with thrombocytopenia syndrome in Chinese individuals.
abstract: BACKGROUND
  Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel bunyavirus named SFTS virus (SFTSV). We hypothesize that host genetic variations may contribute to susceptibility to SFTS.
  
  
  RESULTS
  Compared with the rs1800818 AA genotype, AG + GG genotypes were significantly associated with increased susceptibility to SFTS (odds ratio, 1.66, 95% confidence interval = 1.28-2.16; P < 0.001). By using the ELISA assay, we observed that PDGF-BB concentration was significantly reduced in acute phase of patients than in the controls (P < 0.001) and recovered patients at 6 month (P = 0.007) and 12 month (P = 0.003). A persistently reduced PDGF-BB was also revealed from the SFTSV-infected C57BL/6J mice (P < 0.001). The rs1800818 G allele was associated with decreased serum PDGF-BB levels in SFTS patients at their early infection (P = 0.015). In accordance, the relative mRNA levels of the at-risk G allele of 1800818 were lower than those of the A allele in heterozygous cell from acute phase of SFTS patients. PDGF-B rs1800818 conferred no susceptibility to severe or fatal outcome in SFTS patients.
  
  
  METHODS
  An initially small-scale case-control association study guided the selection of platelet derived growth factor-B (PDGF-B) rs1800818 in 1020 SFTS patients and 1353 controls. Functional analyses were conducted to verify the biological significance of rs1800818 polymorphism.
  
  
  CONCLUSIONS
  Our findings suggest that the PDGF-B rs1800818 polymorphism might play a role in mediating the susceptibility to SFTS.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.
authors.forename: Xiao-Ai
authors.initials: XA
authors.lastname: Zhang
authors.affiliation: Graduate School of Anhui Medical University, Hefei, 230032, P. R. China.
authors.forename: Chen-Tao
authors.initials: CT
authors.lastname: Guo
authors.affiliation: School of Public Health, Peking University, Beijing, 100191, P. R. China.
authors.forename: Qing-Bin
authors.initials: QB
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.
authors.forename: Jian-Gong
authors.initials: JG
authors.lastname: Hu
authors.affiliation: The 154 Hospital, People's Liberation Army, Xinyang, 464000, P. R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Cui
authors.affiliation: The 154 Hospital, People's Liberation Army, Xinyang, 464000, P. R. China.
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Yang
authors.affiliation: The Shangcheng People's Hospital, Xinyang, 464000, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Hu
authors.affiliation: The 154 Hospital, People's Liberation Army, Xinyang, 464000, P. R. China.
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Qin
authors.affiliation: Shandong Provincial Center for Disease Control and Prevention, Jinan, 250001, P. R. China.
authors.forename: Xian-Jun
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Shandong Provincial Center for Disease Control and Prevention, Jinan, 250001, P. R. China.
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.|Graduate School of Anhui Medical University, Hefei, 230032, P. R. China.
authors.forename: Dou-Dou
authors.initials: DD
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.|Graduate School of Anhui Medical University, Hefei, 230032, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, P. R. China.
authors.forename: Wu-Chun
authors.initials: WC
authors.lastname: Cao
mesh_terms: D000818:Animals; D044466:Asian People / Q000235:genetics; D000077214:Becaplermin; D002044:Bunyaviridae Infections / Q000097:blood / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D004195:Disease Models, Animal; D005260:Female; D005334:Fever / Q000097:blood / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood / Q000235:genetics*; D012307:Risk Factors; D012720:Severity of Illness Index; D013577:Syndrome; D013921:Thrombocytopenia / Q000097:blood / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D013997:Time Factors
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin
keywords: PDGF-B; cytokines; polymorphism; severe fever with thrombocytopenia syndrome; susceptibility