title: [Modulatory Effect of Mouse Compact Bone-derived Suspending MSC on T Cells and It's Related Mechanisms].
abstract: OBJECTIVE
  To investigate the modulatory effect of the MSC derived from low attaching culture systems (suspending MSC) on T lymphocytes and the related mechanisms.
  
  
  METHODS
  The suspending MSC were generated from mouse compact bones by using low attaching plates and adherent cell culture flasks, respectively. The morphology of suspending MSC was observed under the inverted microscope and the cells were induced to differentiate into osteoblasts and adipocytes. Further, the surface antigen profile of MSC was analyzed with flow cytometry. In addition, the culture medium (CM) of suspending MSC and adherent MSC was collected and added into the activated T cell cultures before detection of the proliferation by CFSE assay. Moreover, the modulaory effects of the CM on the T cell-derived cytokines were detected by quantitative PCR. Also, the mRNA expression of cytokines of MSC was detected.
  
  
  RESULTS
  The suspending MSC grew in floating cell spheres and differentiated into osteoblasts and adipocytes in the induction medium. Furthermore, the suspending MSC shared the typical immuno-phenotype with their adherent counterparts. In addition, the results of CFSE assay demonstrated that suspending MSC derived CM suppressed ConA induced T cell proliferation. The results of quantitative PCR revealed that suspending MSC expressed transforming factor β1 and interleukin-6 at a higher level and suppressed the T cell expressing interferon γ and interleukine-17A.
  
  
  CONCLUSIONS
  The suspending MSC exerted an unique modulatoy effect on T cells, which is quite different to adherent MSC.
authors.affiliation: Graduate School of Hebei North University, Zhangjiakou 075000, Hebei Province, China.|Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.|Department of Digestion Medicine, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Science and Technology, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Wen-Qing
authors.initials: WQ
authors.lastname: Wu
authors.affiliation: Department of Hematology, Air Force General Hospital of Chinese PLA, Beijing 100142, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Mao
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: zhudingdingabc@163.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Graduate School of Hebei North University, Zhangjiakou 075000, Hebei Province, China.|Department of Digestion Medicine, Air Force General Hospital of Chinese PLA, Beijing 100142, China. E-mail: ning-shoubin@163.com.
authors.forename: Shou-Bin
authors.initials: SB
authors.lastname: Ning
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D017077:Culture Media, Conditioned; D005434:Flow Cytometry; D016130:Immunophenotyping; D015850:Interleukin-6 / Q000378:metabolism; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D015850:Interleukin-6; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1