title: Vernonanthura polyanthes leaves aqueous extract enhances doxorubicin genotoxicity in somatic cells of Drosophila melanogaster and presents no antifungal activity against Candida spp.
abstract: Vernonanthura polyanthes (Spreng.) A.J. Vega & Dematt. (Asteraceae), known as "assa-peixe", has been used in ethnomedicine for the treatment of various diseases such as bronchitis, pneumonia, hemoptysis, persistent cough, internal abscesses, gastric and kidney stone pain. Moreover, some studies demonstrated that species of Genus Vernonia present antifungal activity. Due to the biological relevance of this species, the aim of this study was to investigate the toxic, genotoxic, antigenotoxic and antifungal potential of V. polyanthes leaves aqueous extract in somatic cells of Drosophila melanogaster or against Candida spp. The aqueous extract of the plant showed no toxic, genotoxic and antigenotoxic activity in the experimental conditions tested using the wing somatic mutation and recombination test (SMART/wing). However, when the extract was associated with doxorubicin, used in this work as a positive control, the mutagenic potential of doxorubicin was enhanced, increasing the number of mutations in D. melanogaster somatic cells. In the other hand, no inhibitory activity against Candida spp. was observed for V. polyanthes leaves aqueous extract using agar-well diffusion assay. More studies are necessary to reveal the components present in the V. polyanthes leaves aqueous extract that could contribute to potentiate the doxorubicin genotoxicity.
authors.affiliation: Universidade Estadual de Goiás - UEG, Câmpus Iporá, Av. R2 Q.1, Jardim Novo Horizonte II, CEP 76200-000, Iporá, GO, Brazil.|Instituto Federal Goiano - IF Goiano, Câmpus de Rio Verde, Av. Sul Goiânia, Km 1, Zona Rural, CEP 75901-970, Rio Verde, GO, Brazil.
authors.forename: I J
authors.initials: IJ
authors.lastname: Guerra-Santos
authors.affiliation: Universidade Estadual de Goiás - UEG, Câmpus Iporá, Av. R2 Q.1, Jardim Novo Horizonte II, CEP 76200-000, Iporá, GO, Brazil.
authors.forename: J D
authors.initials: JD
authors.lastname: Rocha
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas I, Universidade Federal de Goiás - UFG, Campus II, Samambaia, saída para Nerópolis, Km 13, CEP 74001-970, Goiânia, GO, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Vale
authors.affiliation: Universidade Estadual de Goiás - UEG, Câmpus Iporá, Av. R2 Q.1, Jardim Novo Horizonte II, CEP 76200-000, Iporá, GO, Brazil.
authors.forename: W C
authors.initials: WC
authors.lastname: Sousa
authors.affiliation: Departamento de Botânica, Instituto de Ciências Biológicas I, Universidade Federal de Goiás - UFG, Campus II, Samambaia, saída para Nerópolis, Km 13, CEP 74001-970, Goiânia, GO, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Teles
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas I, Universidade Federal de Goiás - UFG, Campus II, Samambaia, saída para Nerópolis, Km 13, CEP 74001-970, Goiânia, GO, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Chen-Chen
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas I, Universidade Federal de Goiás - UFG, Campus II, Samambaia, saída para Nerópolis, Km 13, CEP 74001-970, Goiânia, GO, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Carvalho
authors.affiliation: Universidade Estadual de Goiás - UEG, Câmpus Iporá, Av. R2 Q.1, Jardim Novo Horizonte II, CEP 76200-000, Iporá, GO, Brazil.|Universidade Estadual de Goiás - UEG, Câmpus Henrique Santillo, Br 153, 3105, Fazenda Barreiro do Meio, CEP 75132-903, Anápolis, GO, Brazil.
authors.forename: E F L C
authors.initials: EF
authors.lastname: Bailão
mesh_terms: D000818:Animals; D002175:Candida / Q000187:drug effects*; D018929:Cell Culture Techniques; D004249:DNA Damage / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects*; D006822:Hybrid Cells; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D035741:Vernonia
chemical_list: D009153:Mutagens; D010936:Plant Extracts; D004317:Doxorubicin