title: Cell-bound lipases from Burkholderia sp. ZYB002: gene sequence analysis, expression, enzymatic characterization, and 3D structural model.
abstract: The whole-cell lipase from Burkholderia cepacia has been used as a biocatalyst in organic synthesis. However, there is no report in the literature on the component or the gene sequence of the cell-bound lipase from this species. Qualitative analysis of the cell-bound lipase would help to illuminate the regulation mechanism of gene expression and further improve the yield of the cell-bound lipase by gene engineering.
  
  
  
  Three predictive cell-bound lipases, lipA, lipC21 and lipC24, from Burkholderia sp. ZYB002 were cloned and expressed in E. coli. Both LipA and LipC24 displayed the lipase activity. LipC24 was a novel mesophilic enzyme and displayed preference for medium-chain-length acyl groups (C10-C14). The 3D structural model of LipC24 revealed the open Y-type active site. LipA displayed 96 % amino acid sequence identity with the known extracellular lipase. lipA-inactivation and lipC24-inactivation decreased the total cell-bound lipase activity of Burkholderia sp. ZYB002 by 42 % and 14 %, respectively.
  
  
  
  The cell-bound lipase activity from Burkholderia sp. ZYB002 originated from a multi-enzyme mixture with LipA as the main component. LipC24 was a novel lipase and displayed different enzymatic characteristics and structural model with LipA. Besides LipA and LipC24, other type of the cell-bound lipases (or esterases) should exist.
authors.affiliation: National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China. shuzhengyu@fjnu.edu.cn.|Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China. shuzhengyu@fjnu.edu.cn.|College of Life Sciences, Fujian Normal University (Qishan campus), Fuzhou, 350117, China. shuzhengyu@fjnu.edu.cn.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Shu
authors.affiliation: National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|College of Life Sciences, Fujian Normal University (Qishan campus), Fuzhou, 350117, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.affiliation: National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|College of Life Sciences, Fujian Normal University (Qishan campus), Fuzhou, 350117, China.
authors.forename: Shaolei
authors.initials: S
authors.lastname: Shi
authors.affiliation: National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|College of Life Sciences, Fujian Normal University (Qishan campus), Fuzhou, 350117, China.
authors.forename: Xiangduo
authors.initials: X
authors.lastname: Mu
authors.affiliation: National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.|College of Life Sciences, Fujian Normal University (Qishan campus), Fuzhou, 350117, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China. hjz@fjnu.edu.cn.|Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China. hjz@fjnu.edu.cn.|College of Life Sciences, Fujian Normal University (Qishan campus), Fuzhou, 350117, China. hjz@fjnu.edu.cn.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D019117:Burkholderia / Q000145:classification* / Q000201:enzymology*; D003198:Computer Simulation; D004789:Enzyme Activation; D004795:Enzyme Stability; D008049:Lipase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D013045:Species Specificity
chemical_list: D008049:Lipase
keywords: Burkholderia sp. ZYB002; Cell-bound lipase; Lipase LipA; Lipase LipC24