title: Development of a reliable analytical method to determine lipid peroxidation biomarkers in newborn plasma samples.
abstract: This paper describes a reliable analytical method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry to determine F2-isoprostanes and other total byproducts (isoprostanes, isofurans, neuroprostanes and neurofurans) as lipid peroxidation biomarkers in newborn plasma samples. The proposed procedure is characterized by a simple sample treatment employing a reduced sample volume (100µL). Also, it shows a high throughput and high selectivity to determine simultaneously different isoprostane isomers in a large number of samples. The reliability of the described method was demonstrated by analysis of spiked plasma samples, obtaining recoveries between 70% and 130% for most of the analytes. Taking into account the implementation of further clinical studies, it was demonstrated the proper sensitivity of the method by means of the analysis of few human newborn plasma samples. In addition to this, newborn piglet plasma samples (n=80) were analyzed observing that the developed method was suitable to determine the analyte levels present in this kind of samples. Therefore, this analytical method could be applied in further clinical research about establishment of reliable lipid peroxidation biomarkers employing this experimental model.
authors.affiliation: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain. Electronic address: m.consuelo.chafer@uv.es.
authors.forename: C
authors.initials: C
authors.lastname: Cháfer-Pericás
authors.affiliation: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Torres-Cuevas
authors.affiliation: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Sanchez-Illana
authors.affiliation: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Escobar
authors.affiliation: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Kuligowski
authors.affiliation: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway.
authors.forename: R
authors.initials: R
authors.lastname: Solberg
authors.affiliation: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway.
authors.forename: H T
authors.initials: HT
authors.lastname: Garberg
authors.affiliation: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway.
authors.forename: M U
authors.initials: MU
authors.lastname: Huun
authors.affiliation: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway.
authors.forename: O D
authors.initials: OD
authors.lastname: Saugstad
authors.affiliation: Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain; Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Vento
mesh_terms: D015415:Biomarkers; D006801:Humans; D007231:Infant, Newborn; D028421:Isoprostanes; D015227:Lipid Peroxidation; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D028421:Isoprostanes
keywords: Isoprostanes; Liquid chromatography; Mass spectrometry; Newborn; Plasma