title: Transcriptome analysis of reproductive-stage Arabidopsis plants exposed gamma-ray irradiation at various doses.
abstract: Gamma rays (GR) induce significant changes in the structure and expression of genes involved in the regulation of diverse biochemical and physiological processes. Arabidopsis plants exhibit different growth and development patterns in response to exposure to GR. The effects on gene expression of different radiation doses of GR (100 and 800 Gy) administered to Arabidopsis plants were examined at the reproductive stage.
  
  
  
  We irradiated 26-day-old plants with three replications [developmental stages 5.1-6.0, according to Boyes et al. ( 2001 )] using a GR irradiator (60 Co, ca. 150 TBq capacity, Atomic Energy of Canada Limited, Ontario, Canada) at the Korea Atomic Energy Research Institute. Plants were treated with 100, 200, 300, 400, 800, 1200, 1600, or 2000 Gy, and the doses were made from varying the distance to the source.
  
  
  
  We conducted a high-throughput screening analysis and detected 883 GR-responsive genes that showed significant changes; these were involved in several putative metabolic pathways related to biotic stress. Additionally, five overrepresented cis-regulatory elements were identified in the 1-kb upstream regions of GR-responsive genes by using motif enrichment analysis. We also detected three GR-responsive genes associated with stamen development and confirmed their co-regulation with functionally interacting genes.
  
  
  
  This finding suggests that a network-based analysis is a viable approach to identify significant GR-responsive genes associated with the reproductive stage of Arabidopsis. Our results provide further insights into the complex biological systems involved in the response to different doses of GR in plants.
authors.affiliation: a Plant Genomics Laboratory, Department of Applied Plant Sciences , Kangwon National University , Chuncheon , Korea ;
authors.forename: Sun-Goo
authors.initials: SG
authors.lastname: Hwang
authors.affiliation: b NJ Solar Plant Group, NJ Biopia Co. , Gwangju , South Korea ;
authors.forename: Dong Sub
authors.initials: DS
authors.lastname: Kim
authors.affiliation: c Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute , Jeongeup, Jeonbuk , South Korea.
authors.forename: Jin-Baek
authors.initials: JB
authors.lastname: Kim
authors.affiliation: c Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute , Jeongeup, Jeonbuk , South Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Hwang
authors.affiliation: a Plant Genomics Laboratory, Department of Applied Plant Sciences , Kangwon National University , Chuncheon , Korea ;
authors.forename: Hyun Mi
authors.initials: HM
authors.lastname: Park
authors.affiliation: a Plant Genomics Laboratory, Department of Applied Plant Sciences , Kangwon National University , Chuncheon , Korea ;
authors.forename: Jin Hyuk
authors.initials: JH
authors.lastname: Kim
authors.affiliation: a Plant Genomics Laboratory, Department of Applied Plant Sciences , Kangwon National University , Chuncheon , Korea ;
authors.forename: Cheol Seong
authors.initials: CS
authors.lastname: Jang
mesh_terms: D017360:Arabidopsis / Q000502:physiology* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000502:physiology / Q000528:radiation effects; D054817:Pollination / Q000502:physiology*; D011829:Radiation Dosage; D059467:Transcriptome / Q000502:physiology* / Q000528:radiation effects*
chemical_list: D029681:Arabidopsis Proteins
keywords: Arabidopsis; gamma irradiation; plant morphology; transcriptomics