title: Decellularized ovine arteries as biomatrix scaffold support endothelial of mesenchymal stem cells.
abstract: The differentiation rate of adipose-derived mesenchymal stem cells (Ad-MSCs) into endothelial cells is always lower under normal condition, which limits further clinical application of Ad-MSCs for angiogenesis regenerative medicine and needs to be enhanced. In the present study, the tissue-specific-derived decellularized ovine arteries matrix (DCS) was used as scaffold to investigate the pro-endothelial differentiation ability of decellularized ovine arteries matrix as well as the underlying mechanisms. The prepared decellularized ovine arteries matrix by the combination of enzymatic and chemical decellularization approaches preserved macroscopic 3D architecture, native composition and ultrastructure of natural ovine arteries. The RT-PCR, histopathological and immunofluorescence assay results suggested that DCS could increase the proliferation ability of MSC. What's more, the DCS could also induce the endothelial differentiation of MSC, which was further enhanced by adding VEGF. Our results showed that natural 3D matrix from decellularized ovine arteries could induce the endothelial differentiation of AD-MSCs alone or with the combination of VEGF. Our results indicated that the decellularized ovine arteries matrix would serve as an efficient culture system for promoting endothelial differentiation of Ad-MSCs.
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China. zhangwenbo_jade@163.com.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Department of Information, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D010641:Phenotype; D012756:Sheep; D013997:Time Factors; D054457:Tissue Scaffolds; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D042461:Vascular Endothelial Growth Factor A
keywords: Adipose-derived mesenchymal stem cells (Ad-MSCs); Decellularized ovine arteries (DCS); Endothelial differentiation; Regenerative medicine