title: Using CRISPR/Cas to study gene function and model disease in vivo.
abstract: The recent discovery of the CRISPR/Cas system and repurposing of this technology to edit a variety of different genomes have revolutionized an array of scientific fields, from genetics and translational research, to agriculture and bioproduction. In particular, the prospect of rapid and precise genome editing in laboratory animals by CRISPR/Cas has generated an immense interest in the scientific community. Here we review current in vivo applications of CRISPR/Cas and how this technology can improve our knowledge of gene function and our understanding of biological processes in animal models.
authors.affiliation: Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
authors.forename: Darjus F
authors.initials: DF
authors.lastname: Tschaharganeh
authors.affiliation: Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.|Howard Hughes Medical Institute, New York, NY, USA.
authors.forename: Scott W
authors.initials: SW
authors.lastname: Lowe
authors.affiliation: RNAi Core Facility, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
authors.forename: Ralph J
authors.initials: RJ
authors.lastname: Garippa
authors.affiliation: Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
authors.forename: Geulah
authors.initials: G
authors.lastname: Livshits
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D002869:Chromosome Aberrations; D059766:DNA End-Joining Repair; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D063185:Epigenetic Repression; D000072669:Gene Editing / Q000379:methods; D018095:Germ-Line Mutation; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D015533:Transcriptional Activation
keywords: CRISPR/Cas9; cancer; disease models; genome editing; mouse models