title: miR-1275 inhibits adipogenesis via ELK1 and its expression decreases in obese subjects.
abstract: Excessive adipocyte differentiation and proliferation are closely associated with the onset of obesity, which has been partially linked to microRNA expression. In previous studies, using miRNA microarray screening, we found that miR-1275 was significantly decreased in human mature adipocytes. In this study, we examined the role of miR-1275 in adipogenesis. Our results indicated that miR-1275 can inhibit the differentiation of human visceral preadipocytes without affecting their proliferation. ELK1, an E-twenty-six (ETS)-domain transcription factor associated with adipocyte differentiation, was strongly suppressed by miR-1275 in human visceral adipocytes. This was demonstrated via a dual-luciferase reporter assay and pointed to ELK1 as a direct target of miR-1275. Furthermore, miR-1275 expression was significantly diminished in the visceral adipose tissue of overweight and obese human subjects accompanied by a negative correlation with body mass index. These results suggest that miR-1275 could play a future role in the management of obesity, as a novel therapeutic target or biomarker.
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China Institute of PediatricsNanjing Medical University, Nanjing, China.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Pang
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China.
authors.forename: Lianghui
authors.initials: L
authors.lastname: You
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China Institute of PediatricsNanjing Medical University, Nanjing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China Institute of PediatricsNanjing Medical University, Nanjing, China.
authors.forename: Fangyan
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China Institute of PediatricsNanjing Medical University, Nanjing, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China xrguo@njmu.edu.cn chenxiaohui327@hotmail.com.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Children Health CareNanjing Maternity and Child Health Care Hospital Afﬁliated to Nanjing Medical University, Nanjing, China Institute of PediatricsNanjing Medical University, Nanjing, China xrguo@njmu.edu.cn chenxiaohui327@hotmail.com.
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
mesh_terms: D020413:3' Untranslated Regions; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D015415:Biomarkers; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D051767:Early Growth Response Protein 2 / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D050177:Overweight / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D050807:ets-Domain Protein Elk-1 / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D015415:Biomarkers; D051767:Early Growth Response Protein 2; C492214:KLF5 protein, human; D051741:Kruppel-Like Transcription Factors; C000602308:MIRN1275 microRNA, human; D035683:MicroRNAs; D050807:ets-Domain Protein Elk-1
keywords: ELK1; adipogenesis; miR-1275; obesity