title: Characterization of isoform expression and subcellular distribution of MYPT1 in intestinal epithelial cells.
abstract: The regulation of intestinal epithelial permeability requires phosphorylation of myosin regulatory light chain (MLC). The phosphorylation status of MLC is regulated by myosin light chain phosphatase (MLCP) activities. The activity of the catalytic subunit of MLCP (PP1cδ) toward MLC depends on its regulatory subunit (MYPT1). In this study, we revealed the presence of two MYPT1 isoforms, full length and variant 2 in human intestinal (Caco-2) epithelial cells and isolated intestinal epithelial cells (IECs) from mice. In confluent Caco-2 cells, MYPT1 was distributed at cell-cell contacts and colocalized with F-actin. These results suggest that MYPT1 isoforms are expressed in intestinal epithelial cells and MYPT1 may be involved in the regulation of intestinal epithelial barrier function.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China; Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, Suzhou 215123, China.
authors.forename: Juan-Min
authors.initials: JM
authors.lastname: Zha
authors.affiliation: Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, Suzhou 215123, China.
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Li
authors.affiliation: Department of Pathology, The University of Chicago, Chicago 60637, USA.
authors.forename: Yi-Tang
authors.initials: YT
authors.lastname: Wang
authors.affiliation: Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, Suzhou 215123, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: mtao@medmail.com.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Tao
authors.affiliation: Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, Suzhou 215123, China. Electronic address: whe@suda.edu.cn.
authors.forename: Wei-Qi
authors.initials: WQ
authors.lastname: He
mesh_terms: D000199:Actins / Q000378:metabolism; D017398:Alternative Splicing; D000818:Animals; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D043263:Myosin-Light-Chain Phosphatase / Q000235:genetics*; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics
chemical_list: D000199:Actins; D020033:Protein Isoforms; D043263:Myosin-Light-Chain Phosphatase; C490372:PPP1R12A protein, human; C510645:Ppp1r12a protein, mouse
keywords: Epithelial cell; Isoforms; Localization; MYPT1; Splicing