title: Ultrasound-Assisted Enantioselective Esterification of Ibuprofen Catalyzed by a Flower-Like Nanobioreactor.
abstract: A flower-like nanobioreactor was prepared for resolution of ibuprofen in organic solvents. Ultrasound irradiation has been used to improve the enzyme performance of APE1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1) in the enantioselective esterification. Under optimum reaction conditions (ultrasound power, 225 W; temperature, 45 °C; water activity, 0.21), the immobilized APE1547 showed an excellent catalytic performance (enzyme activity, 13.26 μmol/h/mg; E value, 147.1). After ten repeated reaction batches, the nanobioreactor retained almost 100% of its initial enzyme activity and enantioselectivity. These results indicated that the combination of the immobilization method and ultrasound irradiation can enhance the enzyme performance dramatically.
authors.affiliation: College of Horticulture, Jilin Agricultural University, Changchun 130118, China. swabyswaby@163.com.
authors.forename: Baiyi
authors.initials: B
authors.lastname: An
authors.affiliation: College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China. fanhl@jlau.edu.cn.
authors.forename: Hailin
authors.initials: H
authors.lastname: Fan
authors.affiliation: College of Life Science, Jilin Agricultural University, Changchun 130118, China. wuzhuofu@163.com.|Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. wuzhuofu@163.com.
authors.forename: Zhuofu
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. zhengl14@mails.jlu.edu.cn.
authors.forename: Lu
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. w_lei@jlu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. wangzhi@jlu.edu.cn.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science, Jilin Agricultural University, Changchun 130118, China. chg61@163.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
mesh_terms: D041522:Aeropyrum / Q000201:enzymology; D019149:Bioreactors; D004800:Enzymes, Immobilized; D004950:Esterases / Q000378:metabolism*; D004951:Esterification; D019220:High-Energy Shock Waves; D007052:Ibuprofen / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D004950:Esterases; D007052:Ibuprofen
keywords: APE1547; ibuprofen; nanobioreactor; resolution; ultrasound