title: FIRT: Filtered iterative reconstruction technique with information restoration.
abstract: Electron tomography (ET) combining subsequent sub-volume averaging has been becoming a unique way to study the in situ 3D structures of macromolecular complexes. However, information missing in electron tomography due to limited angular sampling is still the bottleneck in high-resolution electron tomography application. Here, based on the understanding of smooth nature of biological specimen, we present a new iterative image reconstruction algorithm, FIRT (filtered iterative reconstruction technique) for electron tomography by combining the algebra reconstruction technique (ART) and the nonlinear diffusion (ND) filter technique. Using both simulated and experimental data, in comparison to ART and weight back projection method, we proved that FIRT could generate a better reconstruction with reduced ray artifacts and significant improved correlation with the ground truth and partially restore the information at the non-sampled angular region, which was proved by investigating the 90° re-projection and by the cross-validation method. This new algorithm will be subsequently useful in the future for both cellular and molecular ET with better quality and improved structural details.
authors.affiliation: Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address: kzhang@mrc-lmb.cam.ac.uk.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Deng
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Shengliu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: zhangfa@ict.ac.cn.
authors.forename: Fa
authors.initials: F
authors.lastname: Zhang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing, China; Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China. Electronic address: feisun@sun5.ibp.ac.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
mesh_terms: D000465:Algorithms; D000818:Animals; D016477:Artifacts; D020755:Coat Protein Complex I / Q000737:chemistry; D004058:Diffusion; D055032:Electron Microscope Tomography / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008099:Liver / Q000737:chemistry; D046911:Macromolecular Substances; D051381:Rats
chemical_list: D020755:Coat Protein Complex I; D046911:Macromolecular Substances
keywords: Algebra reconstruction technique; Electron tomography; Filtered iterative reconstruction technique; Information restoration; Nonlinear diffusion filter