title: Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy.
abstract: The species Deguelia utilis and Deguelia rufescens var. urucu, popularly known as "timbó," have been used for many years as rotenone sources in insecticide formulations. In this work, a method was developed and validated using a high-performance liquid chromatography-photodiode array (HPLC-PDA) system, and results were analyzed using hierarchical cluster analysis (HCA). By quantifying the major rotenoids of these species, it was possible to establish a linear relation between them. The ratio between the concentrations of rotenone and deguelin for D. utilis is approximately 1:0.8, respectively, while for D. rufescens var. urucu it is 2:1. These results may help to distinguish these species contributing to their taxonomic identification.
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. danymont2003@yahoo.com.br.
authors.forename: Danielle
authors.initials: D
authors.lastname: da Costa
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. yumikoyoshioka@yahoo.com.br.
authors.forename: Consuelo
authors.initials: C
authors.lastname: E Silva
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. alinecollares@gmail.com.
authors.forename: Aline
authors.initials: A
authors.lastname: Pinheiro
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. debora.frommenwiler@camag.com.
authors.forename: Débora
authors.initials: D
authors.lastname: Frommenwiler
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. mspa@ufpa.br.
authors.forename: Mara
authors.initials: M
authors.lastname: Arruda
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. giselle@ufpa.br.
authors.forename: Giselle
authors.initials: G
authors.lastname: Guilhon
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. nahum@ufpa.br.
authors.forename: Cláudio
authors.initials: C
authors.lastname: Alves
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. arruda@ufpa.br.
authors.forename: Alberto
authors.initials: A
authors.lastname: Arruda
authors.affiliation: Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-970 Belém-PA, Brazil. yumilton@yahoo.com.br.
authors.forename: Milton
authors.initials: M
authors.lastname: Da Silva
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D007887:Fabaceae / Q000737:chemistry* / Q000145:classification; D007306:Insecticides / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D012402:Rotenone / Q000031:analogs & derivatives / Q000032:analysis*; D013045:Species Specificity
chemical_list: D007306:Insecticides; D012402:Rotenone; C107676:deguelin
keywords: Deguelia; chemotaxonomy; hierarchical cluster analysis (HCA); high-performance liquid chromatography (HPLC); rotenoids