title: Cross-Presentation Assay for Human Dendritic Cells.
abstract: The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules, termed cross-presentation, is essential for the initiation of cytotoxic immune responses. Numerous studies in mice and human have shown that dendritic cells are the best cross-presenting cells. The protocol described here allows the assessment of the cross-presentation by human dendritic cells of a model antigen (either soluble or cell associated) to antigen-specific CD8 T cells.
authors.affiliation: INSERM U932, and Institut Curie, Centre de recherche, 26 rue d'Ulm, 75248, Paris Cedex 05, France. elodie.segura@curie.fr.
authors.forename: Elodie
authors.initials: E
authors.lastname: Segura
mesh_terms: D017951:Antigen Presentation; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D045142:Cross-Priming; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans
keywords: Antigen processing; CD8 T cells; Cross-presentation; Dendritic cells; Human