title: Influence of CD26/dipeptidyl peptidase IV deficiency on immunophenotypic changes during colitis development and resolution.
abstract: A lot of evidence for the importance of CD26/dipeptidyl peptidase IV (CD26/DPP IV) in immunoactivation has been reported; however, its involvement in colitis remains unclear. The aim of this study was to investigate the influence of CD26/DPP IV deficiency on immunophenotypic changes associated with dextran sulfate sodium (DSS)-induced colitis in wild-type (WT) and CD26-deficient mice. Development of clinical symptoms of colitis and animal health status parameters were assessed; the expression of the nuclear factor (NF)-κB p65 subunit was measured by quantitative real-time PCR, while cell characterization was determined by flow cytometry and immunohistochemical staining. DSS treatment induced loss of body weight and colon length shortening in both mouse strains. An increase of myeloperoxidase activity in CD26-deficient mice was more intensive than in WT mice, in spite of similar histopathological changes. Furthermore, a significant increase in the expression of NF-κB p65 subunit in the colon of CD26-deficient mice was determined. The percentage of splenic CD4(+) and CD8(+) cells in the acute phase of colitis was significantly decreased in WT mice, while in the same period, an increase in the percentage of splenic CD8(+) cells was present in CD26-deficient mice. Development of colitis was accompanied by a significant increase in the percentage of intrahepatic NKT cells in both mouse strains, but their percentage in spleen was increased only in CD26-deficient mice. CD26 deficiency was associated with a heightened response to DSS accompanied by increased expression of NF-κB p65 subunit and distinct changes in leukocyte trafficking. These results provide new insights into the role of CD26/DPP IV during the development of colitis.
authors.affiliation: Department of Chemistry and Biochemistry, School of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000, Rijeka, Croatia. dijana.detel@medri.uniri.hr.
authors.forename: Dijana
authors.initials: D
authors.lastname: Detel
authors.affiliation: Department of Chemistry and Biochemistry, School of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000, Rijeka, Croatia.
authors.forename: Suncica
authors.initials: S
authors.lastname: Buljevic
authors.affiliation: Department of Chemistry and Biochemistry, School of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000, Rijeka, Croatia.
authors.forename: Lara Baticic
authors.initials: LB
authors.lastname: Pucar
authors.affiliation: Department of Physiology and Immunology, School of Medicine, University of Rijeka, Rijeka, Croatia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kucic
authors.affiliation: Department of Histology and Embryology, School of Medicine, University of Rijeka, Rijeka, Croatia.
authors.forename: Ester Pernjak
authors.initials: EP
authors.lastname: Pugel
authors.affiliation: Department of Chemistry and Biochemistry, School of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000, Rijeka, Croatia.
authors.forename: Jadranka
authors.initials: J
authors.lastname: Varljen
mesh_terms: D000818:Animals; D015415:Biomarkers; D003093:Colitis, Ulcerative / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D003106:Colon / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016264:Dextran Sulfate; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020556:Neutrophil Infiltration; D009929:Organ Size; D009195:Peroxidase / Q000378:metabolism; D012075:Remission, Spontaneous; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D016264:Dextran Sulfate; D009195:Peroxidase; D018819:Dipeptidyl Peptidase 4; C545245:Dpp4 protein, mouse
keywords: CD26-deficient animals; CD26/dipeptidyl peptidase IV; Dextran sulfate sodium; Immune response; Inflammatory bowel disease