title: Cloning, expression and cytotoxicity of granulin A, a novel polypeptide contained in human progranulin.
abstract: In our previous study, we isolated an antitumor polypeptide, CS5931, from the sea squirt Ciona savignyi; it shares high homology with Ciona intestinalis Granulin A (GRN A). However, little is known about the anticancer effect of GRN A. In the present study, GRN A was cloned and expressed in the yeast Pichia pastoris. The polypeptide was purified to almost homogeneity using a Ni-NTA column. MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] assay reveals that GRN A displays potent cytotoxicity to several human cancer cells. The polypeptide induces cell apoptosis as analyzed by morphological observation and flow cytometry assays. This study provides evidence that GRN A possesses potential to be developed as a novel anticancer agent.
authors.affiliation: Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Huanli
authors.initials: H
authors.lastname: Xu
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
authors.forename: Xiukun
authors.initials: X
authors.lastname: Lin
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003001:Cloning, Molecular; D045325:HCT116 Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D000077153:Progranulins
chemical_list: D000970:Antineoplastic Agents; C492314:GRN protein, human; D036341:Intercellular Signaling Peptides and Proteins; D000077153:Progranulins