title: Zirconium-doped magnetic microspheres for the selective enrichment of cis-diol-containing ribonucleosides.
abstract: Zirconium-doped magnetic microspheres (Zr-Fe3O4) for the selective enrichment of cis-diol-containing biomolecules were easily synthesized via a one-step hydrothermal method. Characterization of the microspheres revealed that zirconium was successfully doped into the lattice of Fe3O4 at a doping level of 4.0 at%. Zr-Fe3O4 possessed good magnetic properties and high specificity towards cis-diol molecules, as shown using 28 compounds. For ribonucleosides, the adsorbent not only has favorable anti-interferential abilities but also has a high adsorption capacity up to 159.4μmol/g. As an example of a real application, four ribonucleosides in urine were efficiently enriched and detected via magnetic solid-phase extraction coupled with high-performance liquid chromatography. Under the optimized extraction conditions, the detection limits were determined to be between 0.005 and 0.017μg/mL, and the linearities ranged from 0.02 to 5.00μg/mL (R≥0.996) for these analytes. The accuracy of the analytical method was examined by studying the relative recoveries of the analytes in real urine samples, with recoveries varying from 77.8% to 119.6% (RSDs<10.6%, n=6). The results indicate that Zr-Fe3O4 is a suitable adsorbent for the analysis of cis-diol-containing biomolecules in practical applications.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710127, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Fan
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710127, PR China.
authors.forename: Peihong
authors.initials: P
authors.lastname: Chen
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710127, PR China.
authors.forename: Chaozhan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710127, PR China. Electronic address: ymwei@nwu.edu.cn.
authors.forename: Yinmao
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D057230:Limit of Detection; D060328:Magnetic Phenomena; D008863:Microspheres; D012263:Ribonucleosides / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D015040:Zirconium / Q000737:chemistry*
chemical_list: D012263:Ribonucleosides; D015040:Zirconium
keywords: Doped magnetic microspheres; Ribonucleosides; Solid-phase extraction; Zirconium; cis-Diol-containing biomolecules