title: Sleep-promoting effects of the GABA/5-HTP mixture in vertebrate models.
abstract: The aim of this study was to investigate the sleep-promoting effect of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) on sleep quality and quantity in vertebrate models. Pentobarbital-induced sleep test and electroencephalogram (EEG) analysis were applied to investigate sleep latency, duration, total sleeping time and sleep quality of two amino acids and GABA/5-HTP mixture. In addition, real-time PCR and HPLC analysis were applied to analyze the signaling pathway. The GABA/5-HTP mixture significantly regulated the sleep latency, duration (p<0.005), and also increased the sleep quality than single administration of the amino acids (p<0.000). Long-term administration increased the transcript levels of GABAA receptor (1.37-fold, p<0.000) and also increased the GABA content compared with the control group 12h after administration (1.43-fold, p<0.000). Our available evidence suggests that the GABA/5-HTP mixture modulates both GABAergic and serotonergic signaling. Moreover, the sleep architecture can be controlled by the regulation of GABAA receptor and GABA content with 5-HTP.
authors.affiliation: Department of Public Health Sciences, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Ki-Bae
authors.initials: KB
authors.lastname: Hong
authors.affiliation: Dongguk University Research Institute of Biotechnology, Goyang 10326, Republic of Korea.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Public Health Sciences, Korea University, Seoul 136-713, Republic of Korea. Electronic address: suh1960@korea.ac.kr.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
mesh_terms: D006916:5-Hydroxytryptophan / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004359:Drug Therapy, Combination; D004569:Electroencephalography; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D008813:Mice, Inbred ICR; D023421:Models, Animal; D010424:Pentobarbital / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism; D012890:Sleep / Q000187:drug effects*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D006993:Hypnotics and Sedatives; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D006916:5-Hydroxytryptophan; D010424:Pentobarbital
keywords: 5-Hydroxytryptophan; Electroencephalogram; Pentobarbital; Sleep; Vertebrate; γ-Aminobutyric acid