title: ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2.
abstract: The Type III secretion system (TTSS) is essential for the intracellular replication of Edwardsiella tarda in phagocytes of fish and mammals, and a hypothetical gene (orf13) located in the TTSS gene cluster is required for intracellular replication and virulence of E. tarda. Here, we show that under TTSS-inducing conditions, the protein ORF13 was secreted into culture supernatant. Then, using a yeast 2-hybrid screen, we show that the mammalian factor Cugbp2, which regulates apoptosis in breast cancer cells, directly interacts with ORF13. A pull-down assay revealed that ORF13 binds to the C-terminal region of Cugbp2. Our results suggest that ORF13 may facilitate E. tarda replication in phagocytes by binding to Cugbp2.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Okuda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Masashi
authors.initials: M
authors.lastname: Yasuda
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakai
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D000067878:CELF Proteins / Q000235:genetics / Q000378:metabolism; D020609:Edwardsiella tarda / Q000235:genetics / Q000378:metabolism*; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology; D005432:Flounder; D015964:Gene Expression Regulation, Bacterial; D008322:Mammals; D051379:Mice; D016601:RNA-Binding Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000069296:Type III Secretion Systems / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D000067878:CELF Proteins; C544452:Celf2 protein, mouse; D016601:RNA-Binding Proteins; D000069296:Type III Secretion Systems