title: Whole Exome Sequencing of Chronic Myeloid Leukemia Patients.
abstract: BACKGROUND
  Previous studies have shown that leukemogenic chromosomal translocations, including fusions between Break point Cluster Region (BCR) and Abelson (ABL) are present in the peripheral blood of healthy individuals. The aim of this study was to gain insights into the genetic alterations other than BCR-Abl translocation in molecular level, which cause chronic myeloid leukemia (CML).
  
  
  METHODS
  We performed whole-exome sequencing on four cases representative of BCR-ABL positive CML in chronic phase of the disease.
  
  
  RESULTS
  We did not identify any pathogenic mutation in all known genes involved in CML or other cancers in our subjects. Nevertheless, we identified polymorphisms in related genes.
  
  
  CONCLUSIONS
  It is the first report of exome sequencing in Philadelphia chromosome positive CML patients. We did not identify any pathogenic mutation in known cancer genes in our patients who can be due to CML pathogenesis or technical limitations.
authors.affiliation: Dept. of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Sabri
authors.affiliation: Hematology and Oncology Research Center, Vali Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Manouchehr
authors.initials: M
authors.lastname: Keyhani
authors.affiliation: Dept. of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Akbari
keywords: CML; Iran; Whole exome sequencing