title: Impact of a short exposure to levofloxacin on faecal densities and relative abundance of total and quinolone-resistant Enterobacteriaceae.
abstract: Emergence of resistant Enterobacteriaceae in the intestinal microbiota during antibiotic treatment is well documented but its early dynamic is not. Here, we compared the densities of total Enterobacteriaceae and relative abundance (RA) of quinolone-resistant Enterobacteriaceae (QRE) in the first stool passed by patients who had a short exposure to levofloxacin (levofloxacin, n=12) or not (control, n=8). Mean densities (SD) (log CFU/g stool) of total Enterobacteriaceae were lower in the levofloxacin group than in the control group-3.4 (1.6) versus 6.7 (1.7), respectively, p <0.001. Conversely, mean RA (SD) of QRE was significantly higher in the levofloxacin group than in the control group-49.7% (23.4) versus 0.1% (3.2), respectively, p <0.05). In conclusion, even a short exposure to levofloxacin has a profound impact on the densities of total Enterobacteriaceae and the QRE-RA.
authors.affiliation: AP-HP, Hôpital Bichat, Service d'Accueil des Urgences, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Bernard
authors.affiliation: AP-HP, Hôpital Bichat, Laboratoire de Bactériologie, Paris, France; INSERM, IAME, UMR 1137, Paris, France; Université Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Armand-Lefèvre
authors.affiliation: AP-HP, Hôpital Bichat, Laboratoire de Bactériologie, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Luce
authors.affiliation: AP-HP, Hôpital Bichat, Laboratoire de Bactériologie, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: El Mniai
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France; Université Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Chau
authors.affiliation: AP-HP, Hôpital Bichat, Service d'Accueil des Urgences, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Casalino
authors.affiliation: AP-HP, Hôpital Bichat, Laboratoire de Bactériologie, Paris, France; INSERM, IAME, UMR 1137, Paris, France; Université Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Andremont
authors.affiliation: AP-HP, Hôpital Bichat, Laboratoire de Bactériologie, Paris, France; INSERM, IAME, UMR 1137, Paris, France; Université Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France. Electronic address: etienne.ruppe@hcuge.ch.
authors.forename: E
authors.initials: E
authors.lastname: Ruppé
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D058491:Bacterial Load; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D006801:Humans; D064704:Levofloxacin / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male
chemical_list: D000900:Anti-Bacterial Agents; D064704:Levofloxacin
keywords: Antibiotic dosage; Antibiotic impact; Antibiotic resistance; Escherichia coli; Fluoroquinolones; Intestinal microbiota; Quantification