title: l-NAME differential effects on diazepam and flunitrazepam responses of rats in the object recognition test.
abstract: BACKGROUND
  The present study was undertaken to better understand possible interaction(s) between a non-selective nitric oxide inhibitor: N(G)-nitro-l-arginine methyl ester (l-NAME) and benzodiazepines (BZs) in recognition memory.
  
  
  METHODS
  The study was carried out on adult male albino Wistar rats. A novel object recognition (NOR) task was used to evaluate memory process.
  
  
  RESULTS
  Combined administration of l-NAME (50mg/kg, ip) with a threshold dose of DZ (0.25mg/kg) induced amnesic effects in rats, participating in the NOR test. On the other hand, following a combined administration of l-NAME (100mg/kg, ip) with flunitrazepam (FNZ; 0.1mg/kg), it was found out that l-NAME inhibited the amnesic effects of FNZ on rats in the NOR test.
  
  
  CONCLUSIONS
  The obtained results suggest that suppressed NO synthesis may lead to a facilitation of DZ-induced memory impairment but surprisingly may prevent amnesic effect after FNZ in rats, submitted to NOR task.
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Lublin, Poland. Electronic address: jolanta.orzelska@umlub.pl.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Orzelska-Gorka
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Lublin, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Talarek
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Lublin, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Listos
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Lublin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Kedzierska
authors.affiliation: Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Lublin, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Fidecka
mesh_terms: D000818:Animals; D003975:Diazepam / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005445:Flunitrazepam / Q000494:pharmacology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D051381:Rats; D021641:Recognition, Psychology / Q000187:drug effects*
chemical_list: D005445:Flunitrazepam; D003975:Diazepam; D019331:NG-Nitroarginine Methyl Ester
keywords: Diazepam; Flunitrazepam; Rat; Recognition memory; l-NAME