title: The long non-coding RNA PARROT is an upstream regulator of c-Myc and affects proliferation and translation.
abstract: Long non-coding RNAs are important regulators of gene expression and signaling pathways. The expression of long ncRNAs is dysregulated in cancer and other diseases. The identification and characterization of long ncRNAs is often challenging due to their low expression level and localization to chromatin. Here, we identify a functional long ncRNA, PARROT (Proliferation Associated RNA and Regulator Of Translation) transcribed by RNA polymerase II and expressed at a relatively high level in a number of cell lines. The PARROT long ncRNA is associated with proliferation in both transformed and normal cell lines. We characterize the long ncRNA PARROT as an upstream regulator of c-Myc affecting cellular proliferation and translation using RNA sequencing and mass spectrometry following depletion of the long ncRNA. PARROT is repressed during senescence of human mammary epithelial cells and overexpressed in some cancers, suggesting an important association with proliferation through regulation of c-Myc. With this study, we add to the knowledge of cytoplasmic functional long ncRNAs and extent the long ncRNA-Myc regulatory network in transformed and normal cells.
authors.affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.|Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors.forename: Dubravka
authors.initials: D
authors.lastname: Vučićević
authors.affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.|EMBL, Heidelberg, Germany.
authors.forename: Maja
authors.initials: M
authors.lastname: Gehre
authors.affiliation: Medizinische Hochschule Hannover Institute of Biochemistry, Hannover, Germany.|RNA Biology and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Sonam
authors.initials: S
authors.lastname: Dhamija
authors.affiliation: Department of Clinical Diochemistry, Næstved Hospital, Næstved, Denmark.
authors.forename: Lennart
authors.initials: L
authors.lastname: Friis-Hansen
authors.affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Meierhofer
authors.affiliation: CU Systems Medicine, Würzburg, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Sauer
authors.affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.
authors.forename: Ulf Andersson
authors.initials: UA
authors.lastname: Ørom
mesh_terms: D000072283:A549 Cells; D049109:Cell Proliferation; D016922:Cellular Senescence; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000187:drug effects*; D040901:Proteomics / Q000379:methods; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
chemical_list: C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding; C000625616:long non-coding RNA PARROT, human
keywords: MYC; long ncRNA