title: Oxidative removal of phenol by HRP-immobilized beads and its environmental toxicology assessment.
abstract: Horseradish peroxidase shows potential biological and environmental applications on the removal of phenolic compounds. In the present study, the HRP-immobilized beads were synthesized to detect the efficiency of the removal of phenol at optimum pH and H2O2 concentration. Comparative in vitro cytotoxicity of phenol/treated solutions were evaluated in HeLa, HepG2 and mcf-7 cells by using MTT method along with flow cytometry study for cell viability and cell cycle distributions. The results showed that the toxicity of phenol solutions were greatly reduced after treated by HRP-immobilized beads, and phenol could lead to deactivate of cells in the S phase and preventing them from going into the G2/M checkpoint. In addition, molecular docking study showed that phenol was a valid inhibitor for the cyclin E in the cell cycle and cell metabolism. Thereby, we established a suitable strategy with promising application for efficient harmless removal of phenol, which significantly decreased the cytotoxic impacts of phenol.
authors.affiliation: College of Chemistry, Fuzhou University, Fuzhou 350108, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Chemistry, Fuzhou University, Fuzhou 350108, China.
authors.forename: He
authors.initials: H
authors.lastname: Fang
authors.affiliation: College of Chemistry, Fuzhou University, Fuzhou 350108, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yi
authors.affiliation: College of Chemistry, Fuzhou University, Fuzhou 350108, China.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou 350002, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xie
authors.affiliation: Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou 350002, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Tang
authors.affiliation: College of Chemistry, Fuzhou University, Fuzhou 350108, China. Electronic address: omr0464@sina.com.
authors.forename: Minrui
authors.initials: M
authors.lastname: Ou
authors.affiliation: College of Chemistry, Fuzhou University, Fuzhou 350108, China. Electronic address: xu@fzu.edu.cn.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xu
mesh_terms: D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019927:Cyclin E / Q000037:antagonists & inhibitors; D054750:Ecotoxicology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D061986:MCF-7 Cells; D062105:Molecular Docking Simulation; D010084:Oxidation-Reduction; D019800:Phenol / Q000737:chemistry* / Q000633:toxicity*; D014874:Water Pollutants, Chemical
chemical_list: D019927:Cyclin E; D004800:Enzymes, Immobilized; D014874:Water Pollutants, Chemical; D019800:Phenol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: Biocatalysis; Cytotoxicity; Horseradish peroxidase; Molecular docking; Phenol