title: Proteomic Analysis of a Poplar Cell Suspension Culture Suggests a Major Role of Protein S-Acylation in Diverse Cellular Processes.
abstract: S-acylation is a reversible post-translational modification of proteins known to be involved in membrane targeting, subcellular trafficking, and the determination of a great variety of functional properties of proteins. The aim of this work was to identify S-acylated proteins in poplar. The use of an acyl-biotin exchange method and mass spectrometry allowed the identification of around 450 S-acylated proteins, which were subdivided into three major groups of proteins involved in transport, signal transduction, and response to stress, respectively. The largest group of S-acylated proteins was the protein kinase superfamily. Soluble N-ethylmaleimide-sensitive factor-activating protein receptors, band 7 family proteins and tetraspanins, all primarily related to intracellular trafficking, were also identified. In addition, cell wall related proteins, including cellulose synthases and other glucan synthases, were found to be S-acylated. Twenty four of the identified S-acylated proteins were also enriched in detergent-resistant membrane microdomains, suggesting S-acylation plays a key role in the localization of proteins to specialized plasma membrane subdomains. This dataset promises to enhance our current understanding of the various functions of S-acylated proteins in plants.
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology, AlbaNova University Centre Stockholm, Sweden.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Srivastava
authors.affiliation: Roy J. Carver Biotechnology Centre, Institute for Genomic Biology, University of Illinois Urbana-Champaign Urbana, IL, USA.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Weber
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology, AlbaNova University Centre Stockholm, Sweden.
authors.forename: Erik
authors.initials: E
authors.lastname: Malm
authors.affiliation: Roy J. Carver Biotechnology Centre, Institute for Genomic Biology, University of Illinois Urbana-Champaign Urbana, IL, USA.
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Fouke
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology, AlbaNova University CentreStockholm, Sweden; ARC Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, The University of Adelaide, Waite CampusUrrbrae, SA, Australia.
authors.forename: Vincent
authors.initials: V
authors.lastname: Bulone
keywords: S-acylation; mass spectrometry; palmitoylation; poplar; post-translational modification; spectral counting