title: Carbon material distribution and flux analysis under varying glucose concentrations in hydrogen-producing Clostridium tyrobutyricum JM1.
abstract: Anaerobic glucose metabolism in hydrogen-producing Clostridium tyrobutyricum was investigated in batch culture with varying initial glucose concentrations (27.8-333.6mM). To understand the regulation of metabolism, the carbon material and reduction balances were applied to estimate the carbon flux distribution for the first time, and metabolic flux analysis (MFA) was used to provide qualitative information and guidance for effective metabolic design. The overall flux distribution suggested that C. tyrobutyricum metabolism has a high capacity for the production of butyrate and hydrogen at an initial glucose concentration of 222.4mM, with balanced activities of NADH and ATP.
authors.affiliation: Division of Resource Circulation, Korea Environment Institute, 370 Sicheong-daero, Sejong 30147, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Jo
authors.affiliation: Department of Environmental Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea. Electronic address: elshine@knu.ac.kr.
authors.forename: Woong
authors.initials: W
authors.lastname: Kim
mesh_terms: D002244:Carbon / Q000378:metabolism*; D048014:Clostridium tyrobutyricum / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D064688:Metabolic Flux Analysis / Q000379:methods*; D053858:Metabolic Networks and Pathways
chemical_list: D002244:Carbon; D006859:Hydrogen; D005947:Glucose
keywords: Carbon balance; Clostridium tyrobutyricum; Energy balance; Hydrogen; Metabolic flux analysis