title: Electrochemiluminescence of Supramolecular Nanorods and Their Application in the "On-Off-On" Detection of Copper Ions.
abstract: In this work, an "on-off-on" switch system has been successfully applied through the construction of an electrochemiluminscent biosensor for copper ion (Cu(2+) ) detection based on a new electrochemiluminescence (ECL) emitter of supramolecular nanorods, which was achieved through supramolecular interactions between 3,4,9,10-perylenetetracarboxylic acid (PTCA) and aniline. The initial "signal-on" state with strong and stable ECL emission was obtained by use of the supramolecular nanorods with a new signal amplification strategy involving a co-reaction accelerator. In addition, ECL quencher probes (Fc-NH2 /Cu-Sub/nano-Au) were fabricated by immobilizing aminoferrocene (Fc-NH2 ) on Cu-substrate strand modified Au nanoparticles. The quencher probes were hybridized with the immobilized Cu-enzyme strand to form Cu(2+) -specific DNAzyme. Similarly, the "signal-off" state was obtained by the high quenching effect of Fc-NH2 on the ECL of the excited-state PTCA ((1) PTCA*). As expected, the second "switch-on" state could achieved by incubating with the target Cu(2+) , owing to the Cu(2+) -specific DNAzyme, which was irreversibly cleaved, resulting in the release of the quencher probes from the sensor interface. Herein, on the basis of the ECL intensity changes (ΔIECL ) before and after incubating with the target Cu(2+) , the prepared Cu(2+) -specific DNAzyme-based biosensor was used for the determination of Cu(2+) concentrations with high sensitivity, excellent selectivity, and good regeneration.
authors.affiliation: The Key Laboratory of Eco-environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.R. China.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Lei
authors.affiliation: The Key Laboratory of Eco-environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: The Key Laboratory of Eco-environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.R. China.
authors.forename: Ai
authors.initials: A
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Eco-environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.R. China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Yu
authors.affiliation: The Key Laboratory of Eco-environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.R. China.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
authors.affiliation: The Key Laboratory of Eco-environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.R. China. yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.affiliation: The Key Laboratory of Eco-environments in the Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.R. China. yingzhuo@swu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
keywords: biosensors; copper; electrochemiluminescence; supramolecular nanorods