title: Ultrapure laser-synthesized Si-based nanomaterials for biomedical applications: in vivo assessment of safety and biodistribution.
abstract: Si/SiOx nanoparticles (NPs) produced by laser ablation in deionized water or aqueous biocompatible solutions present a novel extremely promising object for biomedical applications, but the interaction of these NPs with biological systems has not yet been systematically examined. Here, we present the first comprehensive study of biodistribution, biodegradability and toxicity of laser-synthesized Si-SiOx nanoparticles using a small animal model. Despite a relatively high dose of Si-NPs (20 mg/kg) administered intravenously in mice, all controlled parameters (serum, enzymatic, histological etc.) were found to be within safe limits 3 h, 24 h, 48 h and 7 days after the administration. We also determined that the nanoparticles are rapidly sequestered by the liver and spleen, then further biodegraded and directly eliminated in urine without any toxicity effects. Finally, we found that intracellular accumulation of Si-NPs does not induce any oxidative stress damage. Our results evidence a huge potential in using these safe and biodegradable NPs in biomedical applications, in particular as vectors, contrast agents and sensitizers in cancer therapy and diagnostics (theranostics).
authors.affiliation: Aix Marseille Université, INSERM, CRO2 UMR_S911, Faculté de Pharmacie, 27 boul. Jean Moulin, Marseille, France.
authors.forename: Tarek
authors.initials: T
authors.lastname: Baati
authors.affiliation: Aix Marseille Université, CNRS, LP3 UMR 7341, Campus de Luminy, 163 Avenue de Luminy, Case 917, 13288, Marseille Cedex 9, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al-Kattan
authors.affiliation: Aix Marseille Université, INSERM, CRO2 UMR_S911, Faculté de Pharmacie, 27 boul. Jean Moulin, Marseille, France.|Assistance Publique - Hôpitaux de Marseille, Hôpital Timone, 254 rue Saint Pierre, 13385 Marseille, France.
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Esteve
authors.affiliation: Service d'Anatomie et de Cytologie Pathologique, CHU Monastir 5000, Tunisie.
authors.forename: Leila
authors.initials: L
authors.lastname: Njim
authors.affiliation: Aix Marseille Université, CNRS, LP3 UMR 7341, Campus de Luminy, 163 Avenue de Luminy, Case 917, 13288, Marseille Cedex 9, France.
authors.forename: Yury
authors.initials: Y
authors.lastname: Ryabchikov
authors.affiliation: Aix-Marseille Université, CNRS, UMR 7263, Unité Chimie Physique, Prévention des Risques et Nuisances Technologiques, Faculté de Pharmacie, 13385 Marseille Cedex 5, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Chaspoul
authors.affiliation: Laboratoire des substances naturelles, Institut National de Recherche et d'Analyse Physicochimique, Sidi Thabet, 2020 Tunisie.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami
authors.affiliation: Aix Marseille Université, CNRS, LP3 UMR 7341, Campus de Luminy, 163 Avenue de Luminy, Case 917, 13288, Marseille Cedex 9, France.|National Research Nuclear University "MEPhI" (Moscow Engineering Physics Institute), International Laboratory "Bionanophotonics",31 Kashirskoe sh., 115409 Moscow, Russia.
authors.forename: Marc
authors.initials: M
authors.lastname: Sentis
authors.affiliation: Aix Marseille Université, CNRS, LP3 UMR 7341, Campus de Luminy, 163 Avenue de Luminy, Case 917, 13288, Marseille Cedex 9, France.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Kabashin
authors.affiliation: Aix Marseille Université, INSERM, CRO2 UMR_S911, Faculté de Pharmacie, 27 boul. Jean Moulin, Marseille, France.|Assistance Publique - Hôpitaux de Marseille, Hôpital Timone, 254 rue Saint Pierre, 13385 Marseille, France.
authors.forename: Diane
authors.initials: D
authors.lastname: Braguer
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001682:Biological Availability; D007834:Lasers; D008099:Liver / Q000737:chemistry; D051379:Mice; D050997:Nanomedicine / Q000379:methods; D049329:Nanostructures / Q000008:administration & dosage* / Q000009:adverse effects; D012825:Silicon / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics*; D013154:Spleen / Q000737:chemistry; D014131:Trace Elements / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics*; D014556:Urine / Q000737:chemistry
chemical_list: D014131:Trace Elements; D012825:Silicon