title: Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer.
abstract: OBJECTIVE
  This study was to evaluate the diagnostic value of OSR2, VAV3, and PPFIA3 hypermethylation in gastric cancer (GC) patients.
  
  
  METHODS
  By using methylation-specific polymerase chain reaction (MSP), we detected the methylation status in tissue and serum samples from 48 gastric cancer (GC) patients and 25 normal individuals.
  
  
  RESULTS
  We found that OSR2, VAV3, and PPFIA3 were methylated in 70.8% (34/48), 54.2% (26/48), and 60.4% (29/48) of GC tissue, respectively. On the contrary, those genes were barely methylated in their paired paracancerous histological normal tissues (PCHNTs) (all P values < 0.01). We next analyzed the methylated OSR2, VAV3, and PPFIA3 in serum DNA. Compared with 25 normal individuals, those three genes were significantly hypermethylated in GC patients serum samples (all P values < 0.01). Regarding their diagnostic value in serum samples, the combined sensitivity of at least one positive among the three markers in serum was 83.3%, with a specificity of 88%.
  
  
  CONCLUSIONS
  Our test suggested that methylation of OSR2, VAV3, and PPFIA3 genes in serum sample may offer a good alternative in a simple, promising, and noninvasive detection of GC.
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Wen-han
authors.initials: WH
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Zhang-jian
authors.initials: ZJ
authors.lastname: Zhou
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Tian-he
authors.initials: TH
authors.lastname: Huang
authors.affiliation: No. 2 Subsidiary Hospital of No. 4 Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Guo
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Yong-chun
authors.initials: YC
authors.lastname: Song
authors.affiliation: The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
authors.forename: Dong-min
authors.initials: DM
authors.lastname: Chang
mesh_terms: D000328:Adult; D000368:Aged; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D051567:Proto-Oncogene Proteins c-vav / Q000235:genetics*; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D008565:Membrane Proteins; C486680:OSR2 protein, human; D051567:Proto-Oncogene Proteins c-vav; D014157:Transcription Factors; C496621:VAV3 protein, human