title: [Expression of HOXB4, PRDM16 and HOXA9 in Patients with Acute Myeloid Leukemia and Its Clinical Significance].
abstract: OBJECTIVE
  To investigate HOXB4, PRDM16 and HOXA9 gene expression in patients with acute myeloid leukemia (AML) and its clinical significance.
  
  
  METHODS
  Real-time quantitative PCR (RT-qPCR) with SYBR Green assay was used to detect the expression of HOXB4, PRDM16 and HOXA9 gene in AML patients (40 cases), the patients with complete remission (9 cases) and patients with non-malignant hematologic diseases as control (10 cases). The relationship between the expression levels of gene HOXB4, PRDM16, HOXA9 and clinical features was investigated by statistical analysis.
  
  
  RESULTS
  The gene expression levels of HOXB4, PRDM16, HOXA9 in newly diagnosed or relapsed AML patients were significantly higher than those in patients with non-malignant hematologic disease (P < 0.05). It was observed that the expression of HOXB4 gene in newly diagnosed or relapsed patients positively correlates with leukemic blasts in bone marrow (r = 0.39). The expression levels of HOXB4, PRDM16 and HOXA9 positively correlate with each other. There was statistical significance among gene expressions in different phases (newly diagnosed, relapse, remission). No correlation was observed between expression levels of HOXB4, PRDM16, HOXA9 and chromosome risk status. It was noticed that expression levels of HOXB4, PRDM16, HOXA9 genes were lower in the patients achieved remission after two courses of chemotherapy than those in the other. And high expression group of each gene had a lower remission rate than that in the low expression group.
  
  
  CONCLUSIONS
  The expression level of HOXB4, PRDM16, HOXA9 genes and leukemic blasts somewhat correlate with curative effect and prognosis. The expression of HOXB4, PRDM16, HOXA9 genes is higher in newly diagnosed and relapsed leukemia patients, and lower in the patients acquired CR/PR. High expression of HOXB4, PRDM16, HOXA9 genes predicts an adverse prognosis.
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China. E-mail: ctzhao-006@medmail.com.cn.
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Zhao
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Cui
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Shao-Ling
authors.initials: SL
authors.lastname: Wu
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Liu
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Zhong-Guang
authors.initials: ZG
authors.lastname: Cui
authors.affiliation: Department of Hematology, The Affiliated Hospital of Qiingdao University, Qingdao 266071, Shandong Province, China.
authors.forename: Hong-Guo
authors.initials: HG
authors.lastname: Zhao
mesh_terms: D001853:Bone Marrow; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012008:Recurrence; D012074:Remission Induction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C090460:HOXB4 protein, human; D018398:Homeodomain Proteins; C416371:PRDM16 protein, human; D012333:RNA, Messenger; D014157:Transcription Factors; C097899:homeobox protein HOXA9