title: The Multi-Domain Fibroblast/Myocyte Coupling in the Cardiac Tissue: A Theoretical Study.
abstract: Cardiac fibroblast proliferation and concomitant collagenous matrix accumulation (fibrosis) develop during multiple cardiac pathologies. Recent studies have demonstrated direct electrical coupling between myocytes and fibroblasts in vitro, and assessed the electrophysiological implications of such coupling. However, in the living tissues, such coupling has not been demonstrated, and only indirect coupling via the extracellular space is likely to exist. In this study we employed a multi-domain model to assess the modulation of the cardiac electrophysiological properties by neighboring fibroblasts assuming only indirect coupling. Numerical simulations in 1D and 2D human atrial models showed that extracellular coupling sustains a significant impact on conduction velocity (CV) and a less significant effect on the action potential duration. Both CV and the slope of the CV restitution increased with increasing fibroblast density. This effect was more substantial for lower extracellular conductance. In 2D, spiral waves exhibited reduced frequency with increasing fibroblast density, and the propensity of wavebreaks and complex dynamics at high pacing rates significantly increased.
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, Ramat-Aviv, 69978, Tel-Aviv, Israel.
authors.forename: Ariel
authors.initials: A
authors.lastname: Greisas
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, Ramat-Aviv, 69978, Tel-Aviv, Israel. sharonz@eng.tau.ac.il.
authors.forename: Sharon
authors.initials: S
authors.lastname: Zlochiver
mesh_terms: D054849:Cardiac Electrophysiology; D002450:Cell Communication / Q000502:physiology; D003198:Computer Simulation; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D017629:Gap Junctions / Q000502:physiology; D006801:Humans; D008955:Models, Cardiovascular; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*
keywords: Atrial arrhythmia; Extracellular space; Fibroblasts; Numerical simulations