title: Faecal bacterial composition in dairy cows shedding Mycobacterium avium subsp. paratuberculosis in faeces in comparison with nonshedding cows.
abstract: The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by real-time PCR, and faecal bacterial populations were analysed by 454 pyrosequencing of the 16S rRNA gene. The most notable differences between shedding and nonshedding cows were an increase in the genus Psychrobacter and a decrease in the genera Oscillospira, Ruminococcus, and Bifidobacterium in cows infected with M. avium subsp. paratuberculosis. The present study is the first to report the faecal microbial composition in dairy cows infected with M. avium subsp. paratuberculosis.
authors.affiliation: a Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
authors.forename: Marija
authors.initials: M
authors.lastname: Kaevska
authors.affiliation: a Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Videnska
authors.affiliation: b Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 61600 Brno, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Sedlar
authors.affiliation: a Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Bartejsova
authors.affiliation: a Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Kralova
authors.affiliation: a Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Slana
mesh_terms: D000818:Animals; D057138:Bacterial Shedding; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000382:microbiology*; D003612:Dairying; D005243:Feces / Q000382:microbiology*; D005260:Female; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010283:Paratuberculosis / Q000382:microbiology*; D017422:Sequence Analysis, DNA / Q000662:veterinary
keywords: MAP; Mycobacterium avium sous-espèce paratuberculosis; Mycobacterium avium subsp. paratuberculosis; bovin; cattle; composition microbienne fécale; faecal microbial composition; pyrosequencing; pyroséquençage; « MAP »