title: Distribution and Molecular Characterization of Human Adenovirus and Epstein-Barr Virus Infections in Tonsillar Lymphocytes Isolated from Patients Diagnosed with Tonsillar Diseases.
abstract: Surgically removed palatine tonsils provide a conveniently accessible source of T and B lymphocytes to study the interplay between foreign pathogens and the host immune system. In this study we have characterised the distribution of human adenovirus (HAdV), Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) in purified tonsillar T and B cell-enriched fractions isolated from three patient age groups diagnosed with tonsillar hypertrophy and chronic/recurrent tonsillitis. HAdV DNA was detected in 93 out of 111 patients (84%), while EBV DNA was detected in 58 patients (52%). The most abundant adenovirus type was HAdV-5 (68%). None of the patients were positive for HCMV. Furthermore, 43 patients (39%) showed a co-infection of HAdV and EBV. The majority of young patients diagnosed with tonsillar hypertrophy were positive for HAdV, whereas all adult patients diagnosed with chronic/recurrent tonsillitis were positive for either HAdV or EBV. Most of the tonsils from patients diagnosed with either tonsillar hypertrophy or chronic/recurrent tonsillitis showed a higher HAdV DNA copy number in T compared to B cell-enriched fraction. Interestingly, in the majority of the tonsils from patients with chronic/recurrent tonsillitis HAdV DNA was detected in T cells only, whereas hypertrophic tonsils demonstrated HAdV DNA in both T and B cell-enriched fractions. In contrast, the majority of EBV positive tonsils revealed a preference for EBV DNA accumulation in the B cell-enriched fraction compared to T cell fraction irrespective of the patients' age.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Assadian
authors.affiliation: Department of Surgical Sciences, Otorhinolaryngology and Head and Neck Surgery, Uppsala University, Uppsala, Sweden.
authors.forename: Karl
authors.initials: K
authors.lastname: Sandström
authors.affiliation: Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Uppsala University, Uppsala, Sweden.
authors.forename: Kåre
authors.initials: K
authors.lastname: Bondeson
authors.affiliation: Department of Surgical Sciences, Otorhinolaryngology and Head and Neck Surgery, Uppsala University, Uppsala, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Laurell
authors.affiliation: Department of Surgical Sciences, Otorhinolaryngology and Head and Neck Surgery, Uppsala University, Uppsala, Sweden.
authors.forename: Adnan
authors.initials: A
authors.lastname: Lidian
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden.
authors.forename: Catharina
authors.initials: C
authors.lastname: Svensson
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Akusjärvi
authors.affiliation: Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Uppsala University, Uppsala, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Bergqvist
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, Uppsala, Sweden.
authors.forename: Tanel
authors.identifier: 0000-0002-0561-367X
authors.initials: T
authors.lastname: Punga
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis*; D000260:Adenoviruses, Human / Q000472:pathogenicity; D000293:Adolescent; D000328:Adult; D018951:Antigens, CD20 / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000472:pathogenicity; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008214:Lymphocytes / Q000821:virology*; D008297:Male; D014066:Palatine Tonsil / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014069:Tonsillitis / Q000821:virology*; D055815:Young Adult
chemical_list: D018951:Antigens, CD20