title: Rats with Chronic, Stable Pulmonary Hypertension Tolerate Low Dose Sevoflurane Inhalation as Well as Normal Rats Do.
abstract: The effects of low concentration of sevoflurane on right ventricular (RV) function and intracellular calcium in the setting of pulmonary arterial hypertension (PAH) have not been investigated clearly. We aim to study these effects and associated signaling pathways in rats with PAH.
  
  
  
  Hemodynamics were assessed with or without sevoflurane inhalation in established PAH rats. We analysis the classic RV function parameters and RV-PA coupling efficiency using steady-state PV loop recordings. The protein levels of SERCA2, PLB and p-PLB expression was analyzed by western blot to assess their relevance in PAH.
  
  
  
  Rats with PAH presented with RV hypertrophy and increased pulmonary arterial pressure. The values of Ea, R/L ratio, ESP, SW, PRSW, +dP/dtmax and the slope of the dP/dtmax-EDV relationship increased significantly in PAH rats (P<0.05). Sevoflurane induced a concentration-dependent decrease of systemic and pulmonary blood pressure, HR, RV contractility, and increased the R/L ratio in both groups. Sevoflurane reduced the expression of SERCA2 and increased the expression of PLB in both groups. Interestingly, sevoflurane only reduced the p-PLB/PLB ratio in PAH rats, not in normal rats.
  
  
  
  Rats with chronic, stable pulmonary hypertension tolerate low concentrations of sevoflurane inhalation as well as normal rats do. It may be related to the modulation of the SERCA2-PLB signaling pathway.
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Qin
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: E
authors.initials: E
authors.lastname: Wang
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D065627:Familial Primary Pulmonary Hypertension / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology; D006976:Hypertension, Pulmonary / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D017380:Hypertrophy, Right Ventricular / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage*; D011651:Pulmonary Artery / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism; D000077149:Sevoflurane; D018497:Ventricular Dysfunction, Right / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D016278:Ventricular Function, Right / Q000187:drug effects / Q000502:physiology
chemical_list: C506948:Atp2a2 protein, rat; D002135:Calcium-Binding Proteins; D008738:Methyl Ethers; D000077149:Sevoflurane; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases