title: Long noncoding RNA BDNF-AS protects local anesthetic induced neurotoxicity in dorsal root ganglion neurons.
abstract: BACKGROUND
  Long noncoding RNA (lncRNA) BDNF-AS was recently identified to regulate neurotrophin signaling pathway. In this study, we examined the functional role of BDNF-AS in regulating local anesthetic-induced neurotoxicity in dorsal root ganglion (DRG) neurons.
  
  
  METHODS
  Neonatal mouse DRG neurons were cultured in vitro, and treated with local anesthetic, bupivacaine, to induce neurotoxicity. The corresponding change in BDNF-AS expression level in DRG was probed by qRT-PCR. BDNF-AS was knocked down by siRNA in DRG. The effects of BDNF-AS downregulation on neurite regrowth, neuronal apoptosis and activating TrkB signaling pathway in bupivacaine-injured DRG neurons were probed by neurite outgrowth assay, TUNEL assay and western blot assay, respectively.
  
  
  RESULTS
  During the process of bupivacaine-induce neurotoxicity in DRG, BDNF-AS was significantly upregulated in both dosage- and time- dependent manners. In DRG neurons, siRNA-mediated BDNF-AS downregulation promoted neurite outgrowth, reduced neuronal apoptosis, and phosphorylated TrkB signaling pathway after bupivacaine-induce neurotoxicity.
  
  
  CONCLUSIONS
  BDNF-AS downregulation rescued local anesthetic-induce neurotoxicity in DRG neurons, probably through the activation of neurotrophin TrkB signaling pathway.
authors.affiliation: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Anesthesiology, Hunan Provincial People's Hospital, Changsha 410002, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Hunan Children's Hospital, Changsha 410007, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Emergency, Hunan Provincial People's Hospital, Changsha 410002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Anesthesiology, Hunan Provincial People's Hospital, Changsha 410002, China.
authors.forename: Gaoyin
authors.initials: G
authors.lastname: Kong
authors.affiliation: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: chengwei.song@aol.com.
authors.forename: Chunshui
authors.initials: C
authors.lastname: Lin
mesh_terms: D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002045:Bupivacaine / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D005727:Ganglia, Spinal / Q000473:pathology*; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D018696:Neuroprotective Agents / Q000378:metabolism*; D009498:Neurotoxins / Q000633:toxicity*; D010766:Phosphorylation / Q000187:drug effects; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D020813:Receptor, trkB / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000779:Anesthetics, Local; D019208:Brain-Derived Neurotrophic Factor; D018696:Neuroprotective Agents; D009498:Neurotoxins; D062085:RNA, Long Noncoding; C000613726:long non-coding RNA Mhrt, rat; D020813:Receptor, trkB; D002045:Bupivacaine
keywords: BDNF-AS; Dorsal root ganglia neuron; Local anesthetic; Neurotoxicity; lncRNA