title: Effects of a traditional Chinese medicine, Longdanxiegan formula granule, on Toll-like receptor pathway in female guinea pigs with recurrent genital herpes.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effects of Longdanxiegan formula granule (LDXGFG), a Chinese traditional medicine on Toll-like receptor (TLR) pathway in recurrent genital herpes.
  
  
  METHODS
  An experimental recurrent genital herpes model was constructed using herpes guinea pig model. The effect of LDXGFG on expression levels of TLR pathway genes were detected using real-time polymerase chain reaction. Furthermore, the dendritic cells and Langerhans cells were isolated and the TLR pathway genes of these cells were assayed after LDXGFG treatment.
  
  
  RESULTS
  The result suggested two different expression patterns of TLR pathway genes in genital herpes and recurrent genital herpes, including upregulated genes and downregulated genes. TLR1, TLR4, TLR6, TLR7, TLR8, TLR9, and TLR10 showed a significant decrease while, TLR2, TLR3, and TLR5 increased in genital herpes and recurrent genital herpes guinea pigs. Meanwhile, the downregulated genes in genital herpes and recurrent genital herpes were stimulated by LDXGFG. By contrast, the upregulated genes decreased significantly after LDXGFG treatment. In both dendritic cells and Langerhans cells, the TLR pathway genes exhibited same pattern: the LDXGFG corrected the abnormal expression of TLR pathway genes.
  
  
  CONCLUSIONS
  The present results suggest that LDXGFG is an alternative, inexpensive, and lasting-effect medicine for herpes simplex virus 2 infection.
authors.affiliation: Hunan University of Chinese Medicine, Changsha, Hunan, China. Electronic address: 2235695017@qq.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Kuang
authors.affiliation: Hunan University of Chinese Medicine, Changsha, Hunan, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Hunan University of Chinese Medicine, Changsha, Hunan, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Liu
authors.affiliation: The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zou
authors.affiliation: The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Mi
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006168:Guinea Pigs; D006558:Herpes Genitalis / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D007801:Langerhans Cells / Q000187:drug effects / Q000378:metabolism*; D012008:Recurrence; D015398:Signal Transduction / Q000187:drug effects; D051193:Toll-Like Receptors / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D051193:Toll-Like Receptors; C000617511:longdanxiegan
keywords: Longdanxiegan formula granule; Toll-like receptor pathway genes; acyclovir; herpes simplex virus 2; recurrent genital herpes