title: Selection and characterization of DNA aptamers for the development of light-up biosensor to detect Cd(II).
abstract: In order to develop a facile, cost-effective and quick-testing light-up biosensor with excellent specificity for cadmium ions (Cd(II)) detection, a modified selection method based on target-induced release of strands was used to isolate aptamers of Cd (II) with high specificity. Circular Dichroism (CD) data confirmed that one of the selected aptamers underwent a distinct conformational change on addition of Cd (II). A biosensor for Cd(II) was developed based on the Cd(II)-induced release of fluorescence-labeled aptamer from complex with a quencher-labeled short complementary sequence. The sensing platform displayed a Cd(II) concentration-dependent increase of fluorescence intensity in the low micromolar range and had an excellent selectivity in the presence of various interfering metal ions. Such biosensor could potentially be used for the detection of Cd(II) in environmental samples.
authors.affiliation: School of Life Science, Shanghai University, Shanghai 200444, China; Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Life Science, Shanghai University, Shanghai 200444, China; Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Jine
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Life Science, Shanghai University, Shanghai 200444, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. Electronic address: rjpei2011@sinano.ac.cn.
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques; D002104:Cadmium; D002942:Circular Dichroism
chemical_list: D052157:Aptamers, Nucleotide; D002104:Cadmium
keywords: Aptamer; Biosensor; Cadmium; SELEX