title: Microbiome diversity in the sputum of patients with pulmonary tuberculosis.
abstract: TB is a worldwide pandemic. India has the highest burden of TB, with WHO statistics for 2013 giving an estimated incidence figure of 2.1 million cases for India out of a global incidence of 9 million. Microbiota have been shown to be associated with many disease conditions; however, only few studies have been reported for microbiota associated with TB infection. For the first time, we characterized the composition of microbiota of TB patients of India, using high-throughput 16S rRNA gene sequencing and compared it with healthy controls. Phylum-level analysis showed that the relative abundance of Firmicutes and Actinobacteria was significantly higher in TB samples and Neisseria and Veillonella were two dominant genera after Streptococcus. In our study, significantly different core genera in TB and normal population were found as compared with the reported studies. Also, the presence of diverse opportunistic pathogenic microbiota in TB patients increases the complexity and diversity of sputum microbiota. Characterization of the sputum microbiome is likely to provide important pathogenic insights into pulmonary tuberculosis.
authors.affiliation: Diagnostics R&D, Avantor Performance Materials India Limited (formerly RFCL Limited), New Delhi, 110020, India.
authors.forename: P
authors.initials: P
authors.lastname: Krishna
authors.affiliation: Diagnostics R&D, Avantor Performance Materials India Limited (formerly RFCL Limited), New Delhi, 110020, India.
authors.forename: A
authors.initials: A
authors.lastname: Jain
authors.affiliation: School of Studies in Biotechnology, Jiwaji University, Gwalior, 474011 M.P., India. psbisen@gmail.com.
authors.forename: P S
authors.initials: PS
authors.lastname: Bisen
mesh_terms: D000328:Adult; D000368:Aged; D044822:Biodiversity; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D008297:Male; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D064307:Microbiota; D008875:Middle Aged; D009169:Mycobacterium tuberculosis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology*; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S