title: Proteome analysis of human amniotic mesenchymal stem cells (hA-MSCs) reveals impaired antioxidant ability, cytoskeleton and metabolic functionality in maternal obesity.
abstract: Maternal obesity increases the risk of obesity and/or obesity-related diseases in the offspring of animal models. The aim of this study was to identify metabolic dysfunctions that could represent an enhanced risk for human obesity or obesity-related diseases in newborn or in adult life, similar to what occurs in animal models. To this aim, we studied the proteome of 12 obese (Ob-) and 6 non-obese (Co-) human amniotic mesenchymal stem cells (hA-MSCs) obtained from women at delivery by cesarean section (pre-pregnancy body mass index [mean ± SD]: 42.7 ± 7.7 and 21.3 ± 3.3 kg/m(2), respectively). The proteome, investigated by two-dimensional fluorescence difference gel electrophoresis/mass spectrometry, revealed 62 differently expressed proteins in Ob- vs Co-hA-MSCs (P < 0.05), nine of which were confirmed by western blotting. Bioinformatics analysis showed that these 62 proteins are involved in several statistically significant pathways (P < 0.05), including the stress response, cytoskeleton and metabolic pathways. Oxidative stress was shown to be an early triggering factor of tissue fat accumulation and obesity-related disorders in the offspring of obese animal models. Our finding of a reduced stress response in Ob-hA-MSCs suggests that a similar mechanism could occur also in humans. Long-term follow-up studies of newborns of obese mothers are required to verify this hypothesis.
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Capobianco
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.|Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Caterino
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Iaffaldano
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.|Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: Nardelli
authors.affiliation: Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Sirico
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.|Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Del Vecchio
authors.affiliation: Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Martinelli
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.|Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Lucio
authors.initials: L
authors.lastname: Pastore
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.|Dipartimento di Scienze chimiche, Via Cintia, Complesso Monte Sant'Angelo 21, 80126 Naples, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Pucci
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Sacchetti
mesh_terms: D000650:Amnion / Q000166:cytology*; D000975:Antioxidants / Q000032:analysis*; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004798:Enzymes / Q000032:analysis*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D059630:Mesenchymal Stem Cells / Q000737:chemistry*; D009765:Obesity / Q000473:pathology*; D061848:Optical Imaging; D011247:Pregnancy; D011248:Pregnancy Complications / Q000473:pathology*; D020543:Proteome / Q000032:analysis
chemical_list: D000975:Antioxidants; D003598:Cytoskeletal Proteins; D004798:Enzymes; D020543:Proteome