title: Investigation of enteric adenovirus and poliovirus removal by coagulation processes and suitability of bacteriophages MS2 and φX174 as surrogates for those viruses.
abstract: We evaluated the removal of enteric adenovirus (AdV) type 40 and poliovirus (PV) type 1 by coagulation, using water samples from 13 water sources for drinking water treatment plants in Japan. The behaviors of two widely accepted enteric virus surrogates, bacteriophages MS2 and φX174, were compared with the behaviors of AdV and PV. Coagulation with polyaluminum chloride (PACl, basicity 1.5) removed AdV and PV from virus-spiked source waters: the infectious AdV and PV removal ratios evaluated by means of a plaque-forming-unit method were 0.1-1.4-log10 and 0.5-2.4-log10, respectively. A nonsulfated high-basicity PACl (basicity 2.1) removed infectious AdV and PV more efficiently than did other commercially available PACls (basicity 1.5-2.1), alum, and ferric chloride. The MS2 removal ratios tended to be larger than those of AdV and PV, partly because of differences in the hydrophobicities of the virus particles and the sensitivity of the virus to the virucidal activity of PACl; the differences in removal ratios were not due to differences in the surface charges of the virus particles. MS2, which was more hydrophobic than the other viruses, was inactivated during coagulation with PACl. Therefore, MS2 does not appear to be an appropriate surrogate for AdV and PV during coagulation. In contrast, because φX174, like AdV and PV, was not inactivated during coagulation, and because the hydrophobicity of φX174 was similar to or somewhat lower than the hydrophobicities of AdV and PV, the φX174 removal ratios tended to be similar to or somewhat smaller than those of the enteric viruses. Therefore, φX174 is a potential conservative surrogate for AdV and PV during coagulation. In summary, the surface hydrophobicity of virus particles and the sensitivity of the virus to the virucidal activity of the coagulant are probably important determinants of the efficiency of virus removal during coagulation.
authors.affiliation: Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, N13W8, Sapporo 060-8628, Japan. Electronic address: nobutaka@eng.hokudai.ac.jp.
authors.forename: N
authors.initials: N
authors.lastname: Shirasaki
authors.affiliation: Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, N13W8, Sapporo 060-8628, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsushita
authors.affiliation: Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, N13W8, Sapporo 060-8628, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsui
authors.affiliation: Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, N13W8, Sapporo 060-8628, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Marubayashi
authors.affiliation: Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, N13W8, Sapporo 060-8628, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murai
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000536:Aluminum Hydroxide / Q000737:chemistry; D010584:Bacteriophage phi X 174 / Q000302:isolation & purification*; D060766:Drinking Water / Q000821:virology*; D007564:Japan; D017909:Levivirus / Q000302:isolation & purification*; D017955:Poliovirus / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
chemical_list: D060766:Drinking Water; C016213:aluminum oxychloride; D000536:Aluminum Hydroxide
keywords: Bacteriophage; Coagulation; Electrophoretic mobility; Enteric virus; Hydrophobicity; Virus inactivation