title: Expression of guanylate cyclase-C, guanylin, and uroguanylin is downregulated proportionally to the ulcerative colitis disease activity index.
abstract: The transmembrane receptor guanylate cyclase-C (GC-C) signaling pathway has been implicated in several gastrointestinal disorders. Activation of GC-C via guanylin (Gn) and uroguanylin (Ugn) regulates intestinal fluid and electrolyte homeostasis. However, how it regulates the pathogenesis of inflammatory bowel disease (IBD) is still unclear. Here, we investigated the activation of GC-C signaling in ulcerative colitis (UC) of different clinical severities. A total of 60 UC patients and 20 normal controls were recruited. Evaluation of the UC disease activity index (DAI) was performed using a modified Mayo scoring system. The expression of GC-C, Gn and Ugn in the colonic mucosa was measured by quantitative real-time PCR and Western blot. We found that the UC patients had significantly lower expression of GC-C, Gn and Ugn than the controls. Furthermore, there were significant differences for GC-C, Gn and Ugn expression for the UC groups of Grade 1, 2 and 3, and their expression levels were reduced with increases in their DAI. Taken together, our results demonstrate that GC-C, Gn and Ugn are downregulated in UC, and this downregulation is more significant with aggravation of the clinical condition. Therefore, the GC-C signaling pathway may be implicated in the progression of UC.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Lan
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Junkun
authors.initials: J
authors.lastname: Niu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Jiarong
authors.initials: J
authors.lastname: Miao
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Xiangqian
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Kunhua
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming 650032, China.
authors.forename: Yinglei
authors.initials: Y
authors.lastname: Miao
mesh_terms: D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D005768:Gastrointestinal Hormones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D045265:Natriuretic Peptides / Q000235:genetics* / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; C072685:guanylin; C084362:uroguanylin; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C