title: Rapid identification of osmolytes in tropical microalgae and cyanobacteria by (1)H HR-MAS NMR spectroscopy.
abstract: In this study, we report the chemical characterization of 47 tropical microalgae and cyanobacteria by HR-MAS. The generated data confirm the interest of HR-MAS as a rapid screening technique with the major advantage of its easiness. The sample is used as powder of freeze-dried microalgae without any extraction process before acquisition. The spectral fingerprints of strains are then tested as variables for a chemotaxonomy study to discriminate cyanobacteria and dinoflagellates. The individual factor map generated by PCA analysis succeeds in separating the two groups, essentially thanks to the presence of specific carbohydrates. Furthermore, more resolved signals enable to identify many osmolytes. More precisely the characteristics δ of 2-O-alpha-D-glucosylglycerol (GG) are observed in all 21 h-MAS spectra of tropical cyanobacteria. After specific extraction, complementary analysis by 1D and 2D-NMR spectroscopies validates the identification of this osmolyte.
authors.affiliation: Université Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: Claudia
authors.initials: C
authors.lastname: Zea Obando
authors.affiliation: Université Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Linossier
authors.affiliation: Plateforme technologique de Résonance Magnétique Nucléaire et de Résonance Paramagnétique Electronique de L'Université de Bretagne Occidentale, 6 Avenue le Gorgeu, CS93837 Brest Cedex 3, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Kervarec
authors.affiliation: Université de Polynésie Française, UMR-EIO, LabEx CORAIL, BP 6570, 98702 Faa'a, Tahiti, French Polynesia.
authors.forename: Mayalen
authors.initials: M
authors.lastname: Zubia
authors.affiliation: ARDA, 2, Rue Maxime Rivière, 97490 Sainte Clotilde, La Réunion, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Turquet
authors.affiliation: Université Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Faÿ
authors.affiliation: Université Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Rehel
mesh_terms: D000458:Cyanobacteria; D009682:Magnetic Resonance Spectroscopy; D058086:Microalgae; D061286:Osmometry
keywords: Cyanobacteria; Glucosylglycerol; HR MAS; Microalgae; NMR; Tropical