title: Viability, Acid and Bile Tolerance of Spray Dried Probiotic Bacteria and Some Commercial Probiotic Supplement Products Kept at Room Temperature.
abstract: Production of probiotic food supplements that are shelf-stable at room temperature has been developed for consumer's convenience, but information on the stability in acid and bile environment is still scarce. Viability and acid and bile tolerance of microencapsulated Bifidobacterium spp. and Lactobacillus acidophilus and 4 commercial probiotic supplements were evaluated. Bifidobacterium and L. acidophilus were encapsulated with casein-based emulsion using spray drying. Water activity (aw ) of the microspheres containing Bifidobacterium or L. acidophilus (SD GM product) was adjusted to 0.07 followed by storage at 25 °C for 10 wk. Encapsulated Bifidobacterium spp. and Lactobacillus acidophilus and 4 commercial probiotic supplement products (AL, GH, RE, and BM) were tested. Since commercial probiotic products contained mixed bacteria, selective media MRS-LP (containing L-cysteine and Na-propionate) and MRS-clindamycin agar were used to grow Bifidobacterium spp. or L. acidophilus, respectively, and to inhibit the growth of other strains. The results showed that aw had a strong negative correlation with the viability of dehydrated probiotics of the 6 products. Viable counts of Bifidobacterium spp. and L. acidophilus of SD GM, AL, and GH were between 8.3 and 9.2 log CFU/g, whereas that of BM and RE were between 6.7 and 7.3 log CFU/g. Bifidobacterium in SD GM, in AL, and in GH products and L. acidophilus in SD GM, in AL, and in BM products demonstrated high tolerance to acid. Most of dehydrated probiotic bacteria were able to survive in bile environment except L. acidophilus in RE product. Exposure to gastric juice influenced bacterial survivability in subsequent bile environment.
authors.affiliation: Faculty of Food Science and Nutrition, Univ. Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.|School of Biomedical and Health Sciences, Victoria Univ, Werribee campus, P.O. Box 14428, Melbourne, Vic., 8001, Australia.
authors.forename: Dianawati
authors.initials: D
authors.lastname: Dianawati
authors.affiliation: School of Biomedical and Health Sciences, Victoria Univ, Werribee campus, P.O. Box 14428, Melbourne, Vic., 8001, Australia.
authors.forename: Vijay
authors.initials: V
authors.lastname: Mishra
authors.affiliation: School of Biological Science, The Univ. of Hong Kong, Pokfulam Road, Hong Kong.
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Shah
mesh_terms: D000362:Agar; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development*; D001646:Bile; D001647:Bile Acids and Salts / Q000494:pharmacology; D003132:Commerce; D003890:Desiccation / Q000379:methods*; D004339:Drug Compounding; D004356:Drug Storage; D005750:Gastric Juice; D041981:Gastrointestinal Tract; D006801:Humans; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000254:growth & development*; D019936:Probiotics; D013696:Temperature; D014867:Water
chemical_list: D001647:Bile Acids and Salts; D014867:Water; D000362:Agar
keywords: Lactobacillus acidophilus; acid tolerance; bifidobacterium; bile tolerance; commercial probiotic supplements