title: Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90.
abstract: The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels were elevated when the parasites were incubated at 43°C for 1 hr.
authors.affiliation: Laboratory of Veterinary Small Animal Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tsuboi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Taniyama
authors.forename: Naohiro
authors.initials: N
authors.lastname: Uchida
authors.forename: Reeko
authors.initials: R
authors.lastname: Sato
authors.forename: Kensuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohta
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Takiguchi
mesh_terms: D001403:Babesia / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D017125:Genes, Protozoan / Q000235:genetics / Q000502:physiology; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology; D018869:Heat-Shock Response / Q000502:physiology*; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny
chemical_list: D018841:HSP90 Heat-Shock Proteins