title: Evaluation of Potential Fitness Costs Associated With eCry3.1Ab Resistance in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae).
abstract: Both an eCry3.1Ab-selected and paired control western corn rootworm, Diabrotica virgifera virgifera LeConte, colony were tested for adult longevity, egg oviposition, egg viability, and larval development in order to evaluate the potential fitness costs associated with eCry3.1Ab resistance. Adult longevity experiments were conducted by pairing virgin males and females together in plastic boxes supplied with food, water, and ovipositional medium and observed for survival time. Eggs were also collected from the ovipositional medium once a week to determine average egg oviposition and egg viability. Larval development time experiments were conducted by infesting seedling assays with 25 neonate larvae and recording larval recovery after several days. Adult longevity, average egg oviposition, and larval development time results indicated a lack of fitness costs associated with eCry3.1Ab resistance in the western corn rootworm. Results of egg viability indicated a fitness advantage for the eCry3.1Ab-selected colony with a significantly higher egg hatch than the control.
authors.affiliation: USDA-ARS, 1503 S. Providence, Research PK, Columbia, MO 65211 (rwg5h8@mail.missouri.edu), rwg5h8@mail.missouri.edu.
authors.forename: Ryan W
authors.initials: RW
authors.lastname: Geisert
authors.affiliation: USDA-ARS, 205 Curtis Hall, University of Missouri, Columbia, MO 65211 (Bruce.Hibbard@ars.usda.gov).
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Hibbard
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D001517:Coleoptera / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D004731:Endotoxins / Q000494:pharmacology*; D056084:Genetic Fitness; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007305:Insecticide Resistance; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D008136:Longevity / Q000187:drug effects; D010058:Oviposition / Q000187:drug effects; D010063:Ovum / Q000187:drug effects; D010572:Pest Control, Biological
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Bt resistance; insect resistance management