title: Improved aqueous extraction of microalgal lipid by combined enzymatic and thermal lysis from wet biomass of Nannochloropsis oceanica.
abstract: High moisture content in wet algal biomass hinders effective performance of current lipid extraction methods. An improved aqueous extraction method combing thermal and enzymatic lysis was proposed and performed in algal slurry of Nannochloropsis oceanica (96.0% moisture) in this study. In general, cell-wall of N. oceanica was disrupted via thermal lysis and enzymatic lysis and lipid extraction was performed using aqueous surfactant solution. At the optimal conditions, high extraction efficiencies for both lipid (88.3%) and protein (62.4%) were obtained, which were significantly higher than those of traditional hexane extraction and other methods for wet algal biomass. Furthermore, an excessive extraction of polar lipid was found for wet biomass compared with dry biomass. The advantage of this method is to efficiently extract lipids from high moisture content algal biomass and avoid using organic solvent, indicating immense potential for commercial microalgae-based biofuel production.
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, People's Republic of China.
authors.forename: Runzhi
authors.initials: R
authors.lastname: Li
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, People's Republic of China; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, People's Republic of China. Electronic address: liutz@qibebt.ac.cn.
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Liu
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002473:Cell Wall / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D006586:Hexanes / Q000737:chemistry; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D058086:Microalgae / Q000737:chemistry* / Q000378:metabolism; D012997:Solvents / Q000378:metabolism; D058009:Stramenopiles / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D056804:Biofuels; D004798:Enzymes; D006586:Hexanes; D008055:Lipids; D012997:Solvents; D014867:Water; C026385:n-hexane
keywords: Aqueous extraction; Biofuel; Cell disruption; Microalgae; Nannochloropsis