title: Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects.
abstract: BACKGROUND
  Supplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model.
  
  
  METHODS
  The authors examined 5 mm rat calvarial defects treated with β-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were randomly divided into the following treatment groups (n = 5); TCP; TCP plus PDGF-BB; DBBM; DBBM plus PDGF-BB; and untreated empty control. After 45 days, bone formation was evaluated by histomorphometry and fluorescence microscopy.
  
  
  RESULTS
  The authors report that the area of newly formed bone was similar between the empty controls and the two bone substitutes, TCP and DBBM. Supplementation of TCP and DBBM with PDGF-BB had no significant impact on bone formation. Fluorochrome staining revealed no visible changes in the pattern of bone formation in defects filled with TCP and DBBM, irrespective of PDGF-BB. Furthermore, supplementation with PDGF-BB did not influence biomaterial degradation.
  
  
  CONCLUSIONS
  The authors concluded that PDGF-BB had no impact on bone formation and degradation of bone substitutes in the respective rodent models. Thus, possible beneficial effects of PDGF-BB may require other model situations.
authors.affiliation: Department of Surgery and Integrated Clinic, Araçatuba Dental School, UNESP-Univ Estadual Paulista, São Paulo, Brazil.
authors.forename: Eloá R
authors.initials: ER
authors.lastname: Luvizuto
authors.affiliation: Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Division of Oral Surgery, Medical University of Vienna, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Tangl
authors.affiliation: Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Division of Oral Surgery, Medical University of Vienna, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Toni
authors.initials: T
authors.lastname: Dobsak
authors.affiliation: Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Division of Oral Surgery, Medical University of Vienna, Vienna, Austria.|Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Karoline
authors.initials: K
authors.lastname: Reich
authors.affiliation: Department of Preventive, Restorative and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland. reinhard.gruber@meduniwien.ac.at.|Department of Oral Biology, Medical University of Vienna, Vienna, Austria. reinhard.gruber@meduniwien.ac.at.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Gruber
authors.affiliation: Department of Surgery and Integrated Clinic, Araçatuba Dental School, UNESP-Univ Estadual Paulista, São Paulo, Brazil.
authors.forename: Celso K
authors.initials: CK
authors.lastname: Sonoda
authors.affiliation: Department of Anatomy, Araçatuba Dental School, UNESP-Univ Estadual Paulista, São Paulo, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000494:pharmacology*; D002417:Cattle; D008903:Minerals; D010012:Osteogenesis; D010865:Pilot Projects; D051381:Rats
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D008903:Minerals; C485817:beta-tricalcium phosphate
keywords: Bone augmentation; Bone formation; Bone regeneration; Bone substitutes; Demineralized bovine bone mineral; Graft consolidation; Histomorphometry; Platelet-derived growth factor-BB; Rat calvaria defects; β-tricalcium phosphate