title: Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP).
abstract: Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be elucidated. Using global genetic analysis, we evaluated how two commonly studied cell types, U937 and Jurkat, respond to nsEP exposure. We hypothesized that by studying the genetic response of the cells following exposure, we would gain direct insight into the stresses experienced by the cell and in turn better understand the biophysical interaction taking place during the exposure. Using Ingenuity Systems software, we found genes associated with cell growth, movement and development to be significantly up-regulated in both cell types 4 h post exposure to nsEP. In agreement with our hypothesis, we also found that both cell lines exhibit significant biological changes consistent with mechanical stress induction. These results advance nsEP research by providing strong evidence that the interaction of nsEPs with cells involves mechanical stress.
authors.affiliation: Department of Radiological Sciences, University of Texas Health Science Center San Antonio, San Antonio, Texas 78229, United States of America.|General Dynamics IT, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.
authors.forename: Caleb C
authors.identifier: 0000-0001-8962-1097
authors.initials: CC
authors.lastname: Roth
authors.affiliation: Department of Ophthalmology, University of Texas Health Science Center San Antonio, San Antonio, Texas 78229, United States of America.
authors.forename: Randolph D
authors.initials: RD
authors.lastname: Glickman
authors.affiliation: General Dynamics IT, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.
authors.forename: Gleb P
authors.initials: GP
authors.lastname: Tolstykh
authors.affiliation: General Dynamics IT, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.
authors.forename: Larry E
authors.initials: LE
authors.lastname: Estlack
authors.affiliation: Department of Electrical Engineering, University of Southern California, Los Angeles, California, 90089, United States of America.
authors.forename: Erick K
authors.initials: EK
authors.lastname: Moen
authors.affiliation: Bioeffects Division, Air Force Research Laboratory, Radiofrequency Branch, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.|General Dynamics IT, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.
authors.forename: Ibtissam
authors.initials: I
authors.lastname: Echchgadda
authors.affiliation: Bioeffects Division, Air Force Research Laboratory, Optical Radiation Branch, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.
authors.forename: Hope T
authors.initials: HT
authors.lastname: Beier
authors.affiliation: National Research Council Research Associateship Program, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Barnes
authors.affiliation: Bioeffects Division, Air Force Research Laboratory, Radiofrequency Branch, JBSA Fort Sam Houston, San Antonio, Texas 78234, United States of America.
authors.forename: Bennett L
authors.initials: BL
authors.lastname: Ibey
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology; D004560:Electricity; D004563:Electrochemistry; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D036103:Nanotechnology / Q000379:methods*; D013314:Stress, Mechanical