title: Use of lipidomics to investigate sebum dysfunction in juvenile acne.
abstract: Acne is a multifactorial skin disorder frequently observed during adolescence with different grades of severity. Multiple factors centering on sebum secretion are implicated in acne pathogenesis. Despite the recognized role of sebum, its compositional complexity and limited analytical approaches have hampered investigation of alterations specifically associated with acne. To examine the profiles of lipid distribution in acne sebum, 61 adolescents (29 males and 32 females) were enrolled in this study. Seventeen subjects presented no apparent clinical signs of acne. The 44 affected individuals were clinically classified as mild (13 individuals), moderate (19 individuals), and severe (12 individuals) acne. Sebum was sampled from the forehead with Sebutape(TM) adhesive patches. Profiles of neutral lipids were acquired with rapid-resolution reversed-phase/HPLC-TOF/MS in positive ion mode. Univariate and multivariate statistical analyses led to the identification of lipid species with significantly different levels between healthy and acne sebum. The majority of differentiating lipid species were diacylglycerols (DGs), followed by fatty acyls, sterols, and prenols. Overall, the data indicated an association between the clinical grading of acne and sebaceous lipid fingerprints and highlighted DGs as more abundant in sebum from adolescents affected with acne.
authors.affiliation: Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy camera@ifo.it.
authors.forename: Emanuela
authors.identifier: 0000-0001-6633-0449
authors.initials: E
authors.lastname: Camera
authors.affiliation: Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy.
authors.forename: Matteo
authors.identifier: 0000-0001-7517-2315
authors.initials: M
authors.lastname: Ludovici
authors.affiliation: Laboratory of Cheminformatics and Molecular Modeling, Department of Chemistry, Biology, and Biotechnology, University of Perugia, Perugia, Italy.
authors.forename: Sara
authors.identifier: 0000-0001-9691-8323
authors.initials: S
authors.lastname: Tortorella
authors.affiliation: Acne Unit, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy.
authors.forename: Jo-Linda
authors.initials: JL
authors.lastname: Sinagra
authors.affiliation: Acne Unit, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Capitanio
authors.affiliation: Laboratory of Cheminformatics and Molecular Modeling, Department of Chemistry, Biology, and Biotechnology, University of Perugia, Perugia, Italy.
authors.forename: Laura
authors.identifier: 0000-0002-9282-9013
authors.initials: L
authors.lastname: Goracci
authors.affiliation: Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics, San Gallicano Dermatologic Institute (IRCCS), Rome, Italy.
authors.forename: Mauro
authors.identifier: 0000-0003-4899-6639
authors.initials: M
authors.lastname: Picardo
mesh_terms: D000152:Acne Vulgaris / Q000378:metabolism* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D045782:Hemiterpenes; D006801:Humans; D008055:Lipids / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D008297:Male; D000439:Pentanols / Q000737:chemistry / Q000302:isolation & purification; D012629:Sebum / Q000378:metabolism; D012720:Severity of Illness Index; D012867:Skin / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D013261:Sterols / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004075:Diglycerides; D045782:Hemiterpenes; D008055:Lipids; D000439:Pentanols; D013261:Sterols; C009034:prenol
keywords: chemometrics; glycerides; mass spectrometry; skin lipidome