title: Procoagulant activity in stored units of red blood cells.
abstract: The procoagulant activity (PA) of stored units of red blood cells (RBC) increases over time, which is related to the expression/exposure of tissue factor (TF). However, there is a discrepancy between the TF measured and changes in PA observed, suggesting that other blood components contribute to this activity. Our goal was to evaluate changes in PA of stored RBCs and to determine possible contributors to it. RBC units from 4 healthy donors were prepared and stored at 4 °C. On selected days, RBC aliquots were reconstituted with autologous plasma and tested in the thromboelastography assay. Corresponding supernatants were tested in a clotting assay. For all donors, the clotting time (CT) of reconstituted RBC units decreased from ∼3000-4000s on day 1 to ∼1000-1600s on day 30, with the most dramatic changes occurring between days 1 and 5. Anti-TF antibody slightly prolonged the CT. The concentration of TF did not change significantly over time and was within the range of 0.3-2.3 pM. Bovine lactadherin (LTD) prolonged the CT of the RBC (by 2.4-3.4-fold in days 3-5 and by 1.3-1.8-fold at day 30). Anti-TF antibody together with LTD had a cumulative effect on the CT prolongation. CT of supernatants responded to both anti-TF and anti-FXIa antibodies. Three contributors to the PA of stored RBC were identified, i.e. FXIa in solution and phosphatidylserine and TF exposed on blood cells and microparticles. Failure of LTD and antibodies to completely eliminate PA suggests that other components of blood could contribute to it.
authors.affiliation: Department of Biochemistry, University of Vermont, 360 South Park Drive, Room 235A, Colchester, VT 05446, USA. Electronic address: maleshn1@jhu.edu.
authors.forename: Maya
authors.initials: M
authors.lastname: Aleshnick
authors.affiliation: Department of Biochemistry, University of Vermont, 360 South Park Drive, Room 235A, Colchester, VT 05446, USA. Electronic address: jonathan.foley@ucl.ac.uk.
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Foley
authors.affiliation: Department of Medicine, University of Vermont, 360 South Park Drive, Room 235A, Colchester, VT 05446, USA. Electronic address: friederike.keating@uvmhealth.org.
authors.forename: Friederike K
authors.initials: FK
authors.lastname: Keating
authors.affiliation: Department of Biochemistry, University of Vermont, 360 South Park Drive, Room 235A, Colchester, VT 05446, USA. Electronic address: sbutenas@uvm.edu.
authors.forename: Saulius
authors.initials: S
authors.lastname: Butenas
mesh_terms: D001777:Blood Coagulation / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000502:physiology*; D015945:Factor XIa / Q000378:metabolism*; D006801:Humans; D013048:Specimen Handling / Q000379:methods*; D013925:Thromboplastin / Q000378:metabolism*
chemical_list: D013925:Thromboplastin; D015945:Factor XIa
keywords: Factor XIa; Phospholipids; Red blood cells; Thromboelastography; Tissue factor