title: Secretion pathway of liver IGF-1 via JAK2/STAT3 in chick embryo under the monochromatic light.
abstract: This study reveals mechanism of monochromatic light on the IGF-1 secretion of chick embryo liver. The chick embryos were incubated and exposed to continuous red, green, blue light or a dark environment. Compared to other light-treated groups, green light increased IGF-1 and melatonin concentrations both in plasma and liver, and Mel1a, Mel1b and Mel1c receptors expressions in liver but decreased p-JAK2, p-STAT3 and ROS in liver. IGF-1 had a positive correlation with melatonin, but a negative relevance with p-JAK2 and p-STAT3. In vitro, the IGF-1 level in the hepatocyte supernatant was enhanced by melatonin with lower p-JAK2/p-STAT3 and ROS levels, which was suppressed by Mel1c antagonist but not Mel1a/Mel1b or Mel1b antagonists. AG490 (JAK/STAT inhibitor) promoted role of melatonin-Mel1c modulated IGF-1 secretion. These results suggest the antioxidant effect of melatonin mediated the green light-enhanced IGF-1 secretion of chick embryo liver through Mel1c receptor to inhibit the JAK2/STAT3 pathway.
authors.affiliation: a Laboratory of Anatomy of Domestic Animals , College of Animal Medicine, China Agricultural University , Haidian , Beijing , China.
authors.forename: Tuanjie
authors.initials: T
authors.lastname: Wang
authors.affiliation: a Laboratory of Anatomy of Domestic Animals , College of Animal Medicine, China Agricultural University , Haidian , Beijing , China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: a Laboratory of Anatomy of Domestic Animals , College of Animal Medicine, China Agricultural University , Haidian , Beijing , China.
authors.forename: Zixu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: a Laboratory of Anatomy of Domestic Animals , College of Animal Medicine, China Agricultural University , Haidian , Beijing , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: a Laboratory of Anatomy of Domestic Animals , College of Animal Medicine, China Agricultural University , Haidian , Beijing , China.
authors.forename: Yaoxing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D022781:Hepatocytes / Q000378:metabolism / Q000528:radiation effects; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D053614:Janus Kinase 2 / Q000378:metabolism*; D008027:Light; D008099:Liver / Q000378:metabolism* / Q000528:radiation effects; D008550:Melatonin / Q000097:blood / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D044094:Receptors, Melatonin / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D044094:Receptors, Melatonin; D050796:STAT3 Transcription Factor; D007334:Insulin-Like Growth Factor I; D053614:Janus Kinase 2; D008550:Melatonin
keywords: IGF-1; JAK2/STAT3; Monochromatic light; chick embryo; liver