title: Fenton Reaction-Generated Advanced Oxidation Protein Products Induces Inflammation in Human Embryonic Kidney Cells.
abstract: Fenton reaction is a new mechanism able to generate advanced oxidation protein products (AOPPs) by exposing the human serum albumin to the Fenton system. Here, we characterized the effects of Fenton reaction-generated advanced oxidation protein products (AOPP-FR) on the gene transcription of the nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), and interleukin-6 (IL-6) in human embryonic kidney cells (HEK 293). To investigate the effects of AOPP-FR and AOPP-HOCl on transcription of inflammatory genes, the NF-κB, COX-2, and IL-6 luciferase promoter activities were analyzed. AOPP-FR and AOPP-HOCl were able to induce the activation of the gene transcription of NF-κB, COX-2, and IL-6 in HEK 293 cells. However, the effects of AOPP-FR were significantly higher than the effects of AOPP-HOCl in relation to COX-2 and IL-6. AOPP-FR induces the activation of the gene transcription of NF-κB, COX-2, and IL-6 and may represent a novel pathogenic mediator of inflammation in kidney.
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, Brazil. bochiguilherme@gmail.com.|Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil. bochiguilherme@gmail.com.|Centro de Ciências da Saúde, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Prédio 26, Sala 1401, Camobi, 97105-900, Santa Maria, RS, Brasil. bochiguilherme@gmail.com.
authors.forename: Guilherme Vargas
authors.initials: GV
authors.lastname: Bochi
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Vanessa Dorneles
authors.initials: VD
authors.lastname: Torbitz
authors.affiliation: Research Laboratory of Clinical Microbiology, Franciscan University Center, Santa Maria, Brazil.
authors.forename: Roberto Christ Vianna
authors.initials: RC
authors.lastname: Santos
authors.affiliation: Unitat Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Campus de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Monica
authors.initials: M
authors.lastname: Cubillos-Rojas
authors.affiliation: Unitat Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Campus de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: José Luis Rosa
authors.initials: JL
authors.lastname: López
authors.affiliation: Laboratório de Genética e Ecotoxicologia Molecular, Programa de Pós-Graduação em CiênciasAmbientais, Universidade Comunitária da Região de Chapecó, Chapecó, Brazil.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Siebel
authors.affiliation: Nanosciences Postgraduate Program, Franciscan University Center, Santa Maria, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gomes
authors.affiliation: Research Laboratory of Cellular Biophysics and Inflammation, The Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Jarbas Rodrigues
authors.initials: JR
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, Brazil.|Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Rafael Noal
authors.initials: RN
authors.lastname: Moresco
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced*; D015850:Interleukin-6 / Q000235:genetics; D007501:Iron / Q000494:pharmacology; D016328:NF-kappa B / Q000235:genetics; D012709:Serum Albumin / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects*
chemical_list: D062107:Advanced Oxidation Protein Products; C045076:Fenton's reagent; C508600:IL6 protein, human; D015850:Interleukin-6; D016328:NF-kappa B; D012709:Serum Albumin; D006861:Hydrogen Peroxide; D007501:Iron; D051546:Cyclooxygenase 2
keywords: HEK 293; advanced oxidation protein products; fenton reaction; hypochlorous acid inflammation