title: Influence of chirality on catalytic generation of nitric oxide and platelet behavior on selenocystine immobilized TiO2 films.
abstract: As nitric oxide (NO) plays vital roles in the cardiovascular system, incorporating this molecule into cardiovascular stents is considered as an effective method. In the present study, selenocystine with different chirality (i.e., l- and d-selenocystine) was used as the catalytic molecule immobilized on TiO2 films for decomposing endogenous NO donor. The influences of surface chirality on NO release and platelet behavior were evaluated. Results show that although the amount of immobilized l-selenocystine on the surface was nearly the same as that of immobilized d-selenocystine, in vitro catalytic NO release tests showed that l-selenocystine immobilized surfaces were more capable of catalyzing the decomposition of S-nitrosoglutathione and thus generating more NO. Accordingly, l-selenocystine immobilized surfaces demonstrated significantly increased inhibiting effects on the platelet adhesion and activation, when compared to d-selenocystine immobilized ones. Measurement of the cGMP concentration of platelets further confirmed that surface chirality played an important role in regulating NO generation and platelet behaviors. Additionally, using bovine serum albumin and fibrinogen as model proteins, the protein adsorption determined with quartz crystal microbalance showed that the l-selenocystine immobilized surface enhanced protein adsorption. In conclusion, surface chirality significantly influences protein adsorption and NO release, which may have significant implications in the design of NO-generating cardiovascular stents.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Xiaxin
authors.initials: X
authors.lastname: Pan
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Wu
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Han
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Rifang
authors.initials: R
authors.lastname: Luo
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
authors.affiliation: Department of Mechanical Engineering, University of Colorado at Boulder, Boulder, CO 80309, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: wengyj7032@swjtu.edu.cn.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Weng
mesh_terms: D000072283:A549 Cells; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D002384:Catalysis; D002417:Cattle; D003553:Cystine / Q000031:analogs & derivatives* / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D009569:Nitric Oxide / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000737:chemistry*; D015539:Platelet Activation; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D016566:Organoselenium Compounds; C009226:selenocystine; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D009569:Nitric Oxide; D003553:Cystine; D005340:Fibrinogen; D014025:Titanium
keywords: Chirality; Nitric oxide; Platelet activation; Selenocystine