title: Steroid constituents from the soft coral Sinularia microspiculata.
abstract: A methanol extract of the soft coral Sinularia microspiculata revealed five sterols, including two new compounds. Using combined chromatographic and spectroscopic experiments, the new compounds were found to be 7-oxogorgosterol (1) and 16α-hydroxysarcosterol (2). Their structures were determined on the basis of spectroscopic data ((1)H and (13)C NMR, HSQC, HMBC, (1)H-(1)H COSY, NOESY, and FT-ICR-MS) and by comparing obtained results to the values indicated in previous studies. Among the isolated compounds, 3 showed weak cytotoxic effects against HL-60 (IC50  =  89.02  ±  9.93 μM) cell line, whereas 5 was weakly active against HL-60 (IC50  =  82.80  ±  13.65 μM) and SK-Mel2 (IC50  =  72.32  ±  1.30 μM) cell lines.
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , Hanoi , Vietnam.
authors.forename: Nguyen Van
authors.initials: NV
authors.lastname: Thanh
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , Hanoi , Vietnam.
authors.forename: Ninh Thi
authors.initials: NT
authors.lastname: Ngoc
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , Hanoi , Vietnam.
authors.forename: Hoang Le Tuan
authors.initials: Hle T
authors.lastname: Anh
authors.affiliation: b Institute of Marine Environment and Resources (IMER), VAST , Haiphong , Vietnam.
authors.forename: Do Cong
authors.initials: do C
authors.lastname: Thung
authors.affiliation: c Institute of Biotechnology (IBT), VAST , Hanoi , Vietnam.
authors.forename: Do Thi
authors.initials: do T
authors.lastname: Thao
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , Hanoi , Vietnam.
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , Hanoi , Vietnam.
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nam
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , Hanoi , Vietnam.
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.affiliation: a Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST) , Hanoi , Vietnam.
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003684:Dehydrocholesterols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013256:Steroids / Q000008:administration & dosage* / Q000737:chemistry; D014744:Vietnam
chemical_list: C000613809:16alpha-hydroxysarcosterol; C000613808:7-oxogorgosterol; D000970:Antineoplastic Agents; D003684:Dehydrocholesterols; D013256:Steroids; D002784:Cholesterol
keywords: Alcyoniidae; Sinularia microspiculata; soft coral; steroid