title: Synthesis and characterization of biocompatibility of tenorite nanoparticles and potential property against biofilm formation.
abstract: Aim is to assess the anti-biofilm property of tenorite nanoparticles and to study their suitability as a possible coating material for medical implants. Tenorite (CuO) nanoparticles were synthesized by the optimized thermal decomposition method and characterized using TEM, XRD, FTIR and UV-Vis analysis. Their influence on biofilm formation of microbes was studied by growing multi drug resistant bacterial strains in the presence or absence of these nanoparticles at various concentrations. The cytotoxicity of nanoparticles on mammalian cells was studied at the corresponding concentrations. The nanoparticles were found to be uniformly dispersed, spherical shaped and <50 nm in size. They showed various degrees of anti-biofilm property against clinically isolated, biofilm forming multi drug resistant microorganisms such as Staphylococcus aureus, Pseudomonas fluorescens, Burkholderia mallei, Klebsiella pneumoniae, and Escherichia coli. Furthermore, Hep-2 cells showed excellent viability at tenorite nanoparticles concentration toxic to microbial growth. These results indicate that tenorite nanoparticles may be ideal candidates for being utilized as coating on medical implants in general and dental implants in particular.
authors.affiliation: Division of Bioengineering, School of Lifescience and Bioengineering, Incheon National University, Republic of Korea; Central Inter-Disciplinary Research Facility (CIDRF), Mahatma Gandhi Medical College and Research Institute Campus, Puducherry 607402, India; Department of Microbiology, Bharathidasan University, Tiruchirappalli, India.
authors.forename: Davoodbasha
authors.initials: D
authors.lastname: MubarakAli
authors.affiliation: Department of Microbiology and Biotechnology, Presidency College, Chennai, India.
authors.forename: Jegatheesan
authors.initials: J
authors.lastname: Arunkumar
authors.affiliation: Central Inter-Disciplinary Research Facility (CIDRF), Mahatma Gandhi Medical College and Research Institute Campus, Puducherry 607402, India.
authors.forename: Pratheesh
authors.initials: P
authors.lastname: Pooja
authors.affiliation: Central Inter-Disciplinary Research Facility (CIDRF), Mahatma Gandhi Medical College and Research Institute Campus, Puducherry 607402, India.
authors.forename: Gopalakrishnan
authors.initials: G
authors.lastname: Subramanian
authors.affiliation: Department of Microbiology, Bharathidasan University, Tiruchirappalli, India; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Nooruddin
authors.initials: N
authors.lastname: Thajuddin
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
authors.forename: Naiyf S
authors.initials: NS
authors.lastname: Alharbi
keywords: Antibiofilm; Cell viability; Implants; Multi drug resistant; TEM; Tenorite nanoparticle