title: Prototype of Multiplex Bead Assay for Quantification of Three Serum Biomarkers for In Vitro Diagnosis of Endometriosis.
abstract: Endometriosis is a very common disease, affecting 10% of women in the reproductive age. To date, a significant delay between onset of the symptoms and definitive diagnosis is caused by the lack of a reliable non-invasive diagnostic test. Recently, the potential value as diagnostic markers for endometriosis of three proteins (Zn-alpha2-glycoprotein, serum albumin, and complement C3 precursor), has been showed. In this article, we have defined the experimental conditions for the development of a multiplex bead array assay for rapid and simultaneous quantification of these three biomarkers in the serum of patients with endometriosis. Finally, pivotal experiments on a small cohort of patients have confirmed the diagnostic value of this assay. J. Cell. Physiol. 231: 2622-2627, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Centro Italiano Endometriosi, Rome, Italy.
authors.forename: Pietro Giulio
authors.initials: PG
authors.lastname: Signorile
authors.affiliation: Centro Italiano Endometriosi, Rome, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Baldi
mesh_terms: D054392:Adipokines; D000328:Adult; D001681:Biological Assay / Q000379:methods*; D015415:Biomarkers / Q000097:blood*; D002138:Calibration; D002352:Carrier Proteins / Q000097:blood; D016022:Case-Control Studies; D003176:Complement C3 / Q000378:metabolism; D004715:Endometriosis / Q000097:blood* / Q000175:diagnosis*; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D054392:Adipokines; D015415:Biomarkers; D002352:Carrier Proteins; D003176:Complement C3; D006023:Glycoproteins; D012709:Serum Albumin; C488891:AZGP1 protein, human; D000090203:Zn-Alpha-2-Glycoprotein