title: Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube.
abstract: The influence of three different pollen germination media on the transcript profile of Arabidopsis pollen tubes has been assessed by real-time PCR on a selection of cell wall related genes, and by a statistical analysis of microarray Arabidopsis pollen tube data sets. The qPCR assays have shown remarkable differences on the transcript levels of specific genes depending upon the formulation of the germination medium used. With the aid of principal component analysis performed on existing microarray data, a subset of genes has been identified that is more prone to produce diverging transcript levels. A functional classification of those genes showed that the clusters with higher number of members were those for hydrolase activity (based in molecular function) and for cell wall (based in cellular component). Taken together, these results may indicate that the nutrient composition of the pollen germination media influences pollen tube metabolism and that caution must be taken when interpreting transcriptomic data of pollen tubes.
authors.affiliation: BioFIG, Center for Biodiversity, Functional and Integrative Genomics, Biology Department, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n 4169-007 Porto, Portugal. mariocost@fc.up.pt.
authors.forename: Mário Luís
authors.initials: ML
authors.lastname: da Costa
authors.affiliation: BioFIG, Center for Biodiversity, Functional and Integrative Genomics, Biology Department, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n 4169-007 Porto, Portugal. lcpereir@fc.up.pt.
authors.forename: Luís Gustavo
authors.initials: LG
authors.lastname: Pereira
authors.affiliation: BioFIG, Center for Biodiversity, Functional and Integrative Genomics, Biology Department, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n 4169-007 Porto, Portugal. scoimbra@fc.up.pt.
authors.forename: Sílvia
authors.initials: S
authors.lastname: Coimbra
keywords: Arabidopsis thaliana; Arabinogalactan proteins; pollen tube; pollen tube growth media; real-time PCR