title: A genetically immortalized human stem cell line: a promising new tool for Alzheimer's disease therapy.
abstract: Amyloid-β peptides and hyper-phosphorylated tau are the main pathological hallmarks of Alzheimer's disease (AD). Given the recent failure of several large-scale clinical trials and the lack of disease-modifying pharmacological treatments, there is an urgent need to develop alternative therapies. A clinical grade human CTX0E03 neural stem cell line has recently passed phase I trials in people with stroke. However, this cell line has not been investigated in other neurodegenerative disorders. This study investigates the survival of CTX0E03 cells under conditions based on the underlying AD pathology. Cell viability assays showed a concentration dependence of this cell line to the toxic effects of Aβ1-42, but not Aβ1-40, and okadaic acid, a phosphatase 2A inhibitor. Notably, CTX0E03 cell line displayed toxicity at concentrations significantly higher than both rat neural stem cells and those previously reported for primary cultures. These results suggest CTX0E03 cells could be developed for clinical trials in AD patients.
authors.affiliation: King's College London, Wolfson Centre for Age-Related Diseases, London, SE1 1UL, UK; Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom 73170, Thailand.
authors.forename: Nicha
authors.initials: N
authors.lastname: Puangmalai
authors.affiliation: King's College London, Wolfson Centre for Age-Related Diseases, London, SE1 1UL, UK.
authors.forename: Alyma
authors.initials: A
authors.lastname: Somani
authors.affiliation: Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom 73170, Thailand.
authors.forename: Wipawan
authors.initials: W
authors.lastname: Thangnipon
authors.affiliation: King's College London, Wolfson Centre for Age-Related Diseases, London, SE1 1UL, UK.
authors.forename: Clive
authors.initials: C
authors.lastname: Ballard
authors.affiliation: King's College London, Wolfson Centre for Age-Related Diseases, London, SE1 1UL, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Broadstock
keywords: Alzheimer's disease; CTX0E03 cell line; cell viability; neural stem cell; okadaic acid; oligomeric amyloid-ß