title: Growth hormone regulates the expression of UCP2 in myocytes.
abstract: To determine if and how growth hormone (GH) signaling is involved in energy metabolism.
  
  
  
  We used human embryonic kidney TSA201 cells, human H-EMC-SS chondrosarcoma cells, rat L6 skeletal muscle cells, and murine C2C12 skeletal muscle myoblasts to investigate GH-induced expression of uncoupling protein2 (UCP2) to the GHR/JAK/STAT5 pathway by a combination of a reporter assay, electrophoretic mobility shift assay (EMSA), real-time quantitative PCR, Western blotting.
  
  
  
  We demonstrated that the regulation energy metabolism, which was hypothesized to be directly acted on by GH, involves UCP2 via activated STAT5B, a signal transducer downstream of GH. We also showed that the sequence at the -586 'TTCnGA' may function as a novel putative consensus sequence of STAT5s.
  
  
  
  The results suggest that GH regulates energy metabolism directly in myocytes and that UCP2 participates in the signal transduction pathway that functions downstream of the GHR/JAK/STAT.
authors.affiliation: Medicine and Clinical Science, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo 663-8179, Japan. Electronic address: kumif@mukogawa-u.ac.jp.
authors.forename: Kumi
authors.initials: K
authors.lastname: Futawaka
authors.affiliation: Clinical Research Institute for Endocrine and Metabolic Diseases, National Hospital Organization Kyoto Medical Center, Kyoto 612-8555, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tagami
authors.affiliation: Medicine and Clinical Science, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo 663-8179, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fukuda
authors.affiliation: Medicine and Clinical Science, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo 663-8179, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Koyama
authors.affiliation: Medicine and Clinical Science, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo 663-8179, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Nushida
authors.affiliation: Medicine and Clinical Science, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo 663-8179, Japan.
authors.forename: Syoko
authors.initials: S
authors.lastname: Nezu
authors.affiliation: Clinical Research Institute for Endocrine and Metabolic Diseases, National Hospital Organization Kyoto Medical Center, Kyoto 612-8555, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Imamoto
authors.affiliation: Clinical Research Institute for Endocrine and Metabolic Diseases, National Hospital Organization Kyoto Medical Center, Kyoto 612-8555, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kasahara
authors.affiliation: Medicine and Clinical Science, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo 663-8179, Japan; Clinical Research Institute for Endocrine and Metabolic Diseases, National Hospital Organization Kyoto Medical Center, Kyoto 612-8555, Japan; Department of Nephrology and Blood Purification, Institute of Biomedical Research and Innovation, Kobe Medical Frontier Center, Kobe 650-0047, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Moriyama
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002813:Chondrosarcoma / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D004734:Energy Metabolism / Q000235:genetics*; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D013006:Growth Hormone / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D053614:Janus Kinase 2 / Q000378:metabolism*; D053612:Janus Kinases / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D011986:Receptors, Somatotropin / Q000378:metabolism; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D000071246:Uncoupling Protein 2 / Q000235:genetics*
chemical_list: D011986:Receptors, Somatotropin; D050799:STAT5 Transcription Factor; D000071246:Uncoupling Protein 2; D013006:Growth Hormone; D053614:Janus Kinase 2; D053612:Janus Kinases
keywords: Adult growth hormone deficiency; Energy metabolism; Expression of uncoupling protein2; Growth hormone signaling; Myocytes; Obesity; Signal transduction pathway