title: Kaempferia parviflora extract ameliorates the cognitive impairments and the reduction in cell proliferation induced by valproic acid treatment in rats.
abstract: Kaempferia parviflora is a herbal plant whose rhizomes are used in traditional medicine. Investigations of this plant have shown it to have antidepressant activity and to improve learning and memory in animal models. The aim of the present investigation was to determine whether K. parviflora could protect the brain from the impairments in cognition and hippocampal neurogenesis which are caused by valproic acid (VPA). Male Sprague Dawley rats (180-200g) were given once daily K. parviflora extract (100mg/kg) via oral gavage for 21 days. Rats received twice daily intraperitoneal injections of valproic acid (300mg/kg) from days 8 to 21 of the experiment. Spatial memory was tested using the novel object location (NOL) test five days after the end of treatment. Cell proliferation in the sub granular zone (SGZ) of the dentate gyrus was quantified by immunohistochemistry and levels of doublecortin (DCX) were determined by Western blotting. Co-treatment of VPA and K. parviflora prevented the cognitive decline and reduction in proliferating cells caused by VPA. Furthermore, co-treatment significantly increased DCX protein levels within the hippocampus. These findings demonstrate that K. parviflora is able to prevent the brain from VPA-induced the impairments of spatial memory and proliferating cells within the SGZ.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand; Neuroscience Research and Development Group, Khon Kaen University, Khon Kaen, 40002, Thailand; Center for Research and Development of Herbal Health Products, Khon Kaen University, Khon Kaen, 40002, Thailand. Electronic address: jariya@kku.ac.th.
authors.forename: Jariya Umka
authors.initials: JU
authors.lastname: Welbat
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Pornthip
authors.initials: P
authors.lastname: Chaisawang
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Wunnee
authors.initials: W
authors.lastname: Chaijaroonkhanarak
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Parichat
authors.initials: P
authors.lastname: Prachaney
authors.affiliation: Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand; Center for Research and Development of Herbal Health Products, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Wanassanun
authors.initials: W
authors.lastname: Pannangrong
authors.affiliation: Center for Research and Development of Herbal Health Products, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Bungorn
authors.initials: B
authors.lastname: Sripanidkulchai
authors.affiliation: School of Life Sciences, Medical School, Queen's Medical Centre, The University of Nottingham, Nottingham, NG7 2UH, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Wigmore
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D003071:Cognition / Q000187:drug effects*; D003072:Cognition Disorders / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000089662:Doublecortin Protein; D041743:Herb-Drug Interactions; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage; D010936:Plant Extracts / Q000008:administration & dosage; D028321:Plant Preparations / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014635:Valproic Acid; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D018696:Neuroprotective Agents; D010936:Plant Extracts; D028321:Plant Preparations; D014635:Valproic Acid
keywords: Hippocampus; Kaempferia parviflora; Neurogenesis; Spatial memory; Valproic acid