title: Genome characterization of feline morbillivirus from Italy.
abstract: Feline morbillivirus (FeMV) has been recently identified by RT-PCR in the urine sample of a nephropathic cat in Italy. In this report, we describe the whole genome sequence of strain Piuma/2015 obtained by combination of sequence independent single primer amplification method (SISPA) and next generation sequencing (NGS) starting from RNA purified from the infected urine sample. The existence in Germany and Turkey of FeMVs from cats divergent from Piuma/2015, suggests the presence of FeMV heterogeneity in Europe as it has been described previously in Japan and China.
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
authors.forename: Maurilia
authors.initials: M
authors.lastname: Marcacci
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
authors.forename: Eliana
authors.initials: E
authors.lastname: De Luca
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
authors.forename: Guendalina
authors.initials: G
authors.lastname: Zaccaria
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Italy.
authors.forename: Morena
authors.initials: M
authors.lastname: Di Tommaso
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
authors.forename: Iolanda
authors.initials: I
authors.lastname: Mangone
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Aste
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Savini
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Boari
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy. Electronic address: a.lorusso@izs.it.
authors.forename: Alessio
authors.initials: A
authors.lastname: Lorusso
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D017931:DNA Primers; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D007558:Italy; D018103:Morbillivirus / Q000235:genetics* / Q000302:isolation & purification; D018185:Morbillivirus Infections / Q000662:veterinary / Q000821:virology; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D014556:Urine / Q000821:virology
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
keywords: Cat; Feline morbillivirus; Italy; NGS; SISPA; Urine