title: Ectopic expression of amaranth seed storage albumin modulates photoassimilate transport and nutrient acquisition in sweetpotato.
abstract: Storage proteins in plants, because of high nutrient value, have been a subject of intensive investigation. These proteins are synthesized de novo in the cytoplasm and transported to the storage organelles where they serve as reservoir of energy and supplement of nitrogen during rapid growth and development. Sweetpotato is the seventh most important food crop worldwide, and has a significant contribution to the source of nutrition, albeit with low protein content. To determine the behaviour of seed storage proteins in non-native system, a seed albumin, AmA1, was overexpressed in sweetpotato with an additional aim of improving nutritional quality of tuber proteins. Introduction of AmA1 imparted an increase in protein and amino acid contents as well as the phytophenols. The proteometabolomics analysis revealed a rebalancing of the proteome, with no significant effects on the global metabolome profile of the transgenic tubers. Additionally, the slower degradation of starch and cellulose in transgenic tubers, led to increased post-harvest durability. Present study provides a new insight into the role of a seed storage protein in the modulation of photoassimilate movement and nutrient acquisition.
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi-110067, India.|Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India.
authors.forename: Shubhendu
authors.initials: S
authors.lastname: Shekhar
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Lalit
authors.initials: L
authors.lastname: Agrawal
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Mishra
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Assam, India.
authors.forename: Alak Kumar
authors.initials: AK
authors.lastname: Buragohain
authors.affiliation: Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India.
authors.forename: Mullath
authors.initials: M
authors.lastname: Unnikrishnan
authors.affiliation: Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India.
authors.forename: Chokkappan
authors.initials: C
authors.lastname: Mohan
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
mesh_terms: D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D027721:Amaranthus / Q000235:genetics* / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000066630:Ectopic Gene Expression; D018506:Gene Expression Regulation, Plant; D027723:Ipomoea batatas / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D009753:Nutritive Value; D010636:Phenols / Q000032:analysis; D064209:Phytochemicals / Q000032:analysis; D030821:Plants, Genetically Modified / Q000254:growth & development; D040901:Proteomics; D055314:Seed Storage Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000418:Albumins; D000596:Amino Acids; D010636:Phenols; D064209:Phytochemicals; D055314:Seed Storage Proteins