title: Application of ZnO Nanoparticles for Improving the Thermal and pH Stability of Crude Cellulase Obtained from Aspergillus fumigatus AA001.
abstract: Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass. In this study thermal and pH stability of crude cellulase has been investigated in the presence of zinc oxide (ZnO) nanoparticles. We synthesized ZnO nanoparticle by sol-gel method and characterized through various techniques including, X-ray Diffraction, ultraviolet-visible spectroscope, field emission scanning electron microscope and high resolution scanning electron microscope. The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5. These findings may be promising in the area of biofuels production.
authors.affiliation: Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture Technology & SciencesAllahabad, India; Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University)Varanasi, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Srivastava
authors.affiliation: Department of Physics and Astrophysics, University of Delhi Delhi, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Srivastava
authors.affiliation: Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University) Varanasi, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Mishra
authors.affiliation: Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture Technology & Sciences Allahabad, India.
authors.forename: Pramod W
authors.initials: PW
authors.lastname: Ramteke
keywords: Aspergillus fumigatus; biofuels production; cellulase; nanoparticles; pH stability; thermal stability; zinc oxide