title: An N-sulfanylethylanilide-based traceable linker for enrichment and selective labelling of target proteins.
abstract: An N-sulfanylethylanilide-based traceable linker, developed to facilitate identification of target proteins of bioactive compounds, was introduced into an alkynylated target protein. Subsequent adsorption onto streptavidin beads allowed it to be treated with a cysteine-fluorophore conjugate in the presence of phosphate. This induced the N-S acyl transfer reaction of the N-sulfanylethylanilide unit. The subsequent native chemical ligation of the fluorophore resulted in cleavage of the linker for target elution and fluorescence labelling of the target, allowing it to be distinguished from non-target proteins.
authors.affiliation: Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan. shigenaga.akira@tokushima-u.ac.jp aotaka@tokushima-u.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Morisaki
authors.forename: Masaya
authors.initials: M
authors.lastname: Denda
authors.forename: Jun
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tsuji
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Inokuma
authors.forename: Kohji
authors.initials: K
authors.lastname: Itoh
authors.forename: Akira
authors.initials: A
authors.lastname: Shigenaga
authors.forename: Akira
authors.initials: A
authors.lastname: Otaka
mesh_terms: D000327:Adsorption; D000480:Alkynes / Q000737:chemistry; D000813:Anilides / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000480:Alkynes; D000813:Anilides; D012710:Serum Albumin, Bovine; D019809:Streptavidin; D003545:Cysteine; D009584:Nitrogen