title: [Application of Ion Torrent PGM™ System in Detection of Fetal DNA in Maternal Plasma].
abstract: OBJECTIVE
  To explore the feasibility of detecting of Y-STR of fetal DNA in maternal plasma using Ion Torrent PGM™ System.
  
  
  METHODS
  A total of 16 fetal DNA samples from maternal plasmas (8 cases from 38 weeks gestational age and 8 ones from 12 weeks) were prepared and a multiplex assay with 7 STR loci (DYS390, DYS391, DYS393, DYS438, DYS437, DYS456, DYS635) was designed for multiplex-PCR amplification. Using Ion Torrent PGM™ System, the results of Y-STR sequences and capillary electrophoresis were obtained and compared.
  
  
  RESULTS
  Y-STR specific alleles were detected in the maternal plasma of all the pregnant women having male babies of second and third trimester, which were higher than that detected by capillary electrophoresis. Consistent Y-STR genotypes were observed between fetal DNA from maternal plasma and genomic DNA from the newborn babies.
  
  
  CONCLUSIONS
  Based on Ion Torrent PGM™ System, the prenatal Y-STR detection method may provide a high-sensitive and high-throughput choice for prenatal STR detection in forensic testing.
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Liu
authors.forename: Xue-ying
authors.initials: XY
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ping
authors.forename: Qing-wen
authors.initials: QW
authors.lastname: Xu
authors.forename: Jiang-ping
authors.initials: JP
authors.lastname: Huang
authors.forename: Kai-nan
authors.initials: KN
authors.lastname: Zou
authors.forename: Huai-gu
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D000483:Alleles; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004247:DNA / Q000097:blood*; D005190:Family; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D004247:DNA