title: Analysis of silica nanoparticles by capillary electrophoresis coupled to an evaporative light scattering detector.
abstract: A simple and rapid methodology has been developed to identify and separate silica nanoparticles (SiO2NPs) of different sizes in aqueous solution by capillary zone electrophoresis coupled to an evaporative light scattering detector (CE-ELSD). SiO2NPs were separated using 3 mM ammonium acetate buffer, containing 1% methanol at pH 6.9. SiO2NPs of 20, 50 and 100 nm were successfully separated under the optimum experimental conditions. CE coupled to ELSD has been proven to be an effective separation technique to determine particles with such small sizes, although the peaks are very close to each other, and it is a promising technique that may allow the separation of other types of nanoparticles. Confirmation by TEM and quantification of the SiO2 content was also carried out by inductively coupled plasma-mass spectrometry (ICP-MS). The new method was applied to the analysis of real samples, in order to assess its ability to avoid matrix effects in the determination of SiO2NPs in these kinds of samples.
authors.affiliation: Analytical Chemistry and Food Technology Department, University of Castilla-La Mancha, Camilo José Cela Avenue, E-13005, Ciudad Real, Spain; Regional Institute for Applied Chemistry Research, IRICA, Camilo José Cela Avenue, E-13005, Ciudad Real, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Adelantado
authors.affiliation: Analytical Chemistry and Food Technology Department, University of Castilla-La Mancha, Avda. Carlos III, s/n, E-45071, Toledo, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Rodríguez-Fariñas
authors.affiliation: Analytical Chemistry and Food Technology Department, University of Castilla-La Mancha, Avda. Carlos III, s/n, E-45071, Toledo, Spain.
authors.forename: Rosa C
authors.initials: RC
authors.lastname: Rodríguez Martín-Doimeadios
authors.affiliation: Regional Institute for Applied Chemistry Research, IRICA, Camilo José Cela Avenue, E-13005, Ciudad Real, Spain; Castilla-La Mancha Science and Technology Park, E-02006, Albacete, Spain.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Zougagh
authors.affiliation: Analytical Chemistry and Food Technology Department, University of Castilla-La Mancha, Camilo José Cela Avenue, E-13005, Ciudad Real, Spain; Regional Institute for Applied Chemistry Research, IRICA, Camilo José Cela Avenue, E-13005, Ciudad Real, Spain. Electronic address: angel.rios@uclm.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Ríos
keywords: Capillary electrophoresis; Coupling arrangement; Evaporative light scattering detector; Salt samples; Silica nanoparticles; Size separation