title: The effects of cordycepin on the cell proliferation, migration and apoptosis in human lung cancer cell lines A549 and NCI-H460.
abstract: OBJECTIVE
  Our study aimed to evaluate the effect of cordycepin on human lung cancer cell lines A549 and NCI-H460.
  
  
  METHODS
  Human lung cancer A549 cells and NCI-H460 cells were treated with different concentrations of cordycepin for different times. Cells incubated without cordycepin were defined as a control. The cell proliferation, migration and apoptosis were, respectively, determined by MTT assay, transwell migration assay and flow cytometry. Additionally, the expression levels of related proteins associated with cell cycle, epithelial-mesenchymal transition (EMT) and apoptosis were examined.
  
  
  RESULTS
  The survival rate of A549 cells and NCI-H460 cells treated with cordycepin significantly decreased compared with untreated cells in a concentration-dependent manner, while the apoptosis rate increased. The migration number of cells treated with cordycepin significantly decreased as the increase in concentration. qRT-PCR and Western blot analysis showed that the aberrant expression of related molecules associated with cell cycle, migration and apoptosis was observed in the lung cancer cells, such as cyclin B, cyclin E, MMP-9, caspase-3 and Bcl-2.
  
  
  CONCLUSIONS
  Cordycepin may exert inhibitory effects on the development of human lung cancer via inhibiting cell proliferation, suppressing migration and inducing apoptosis, suggesting that cordycepin may have therapeutic potential for the treatment of this disease.
authors.affiliation: Thoracic Department, Changzheng Hospital of Shanghai affiliated to The Secondary Military Medical University, Shanghai, China.
authors.forename: Xiandong
authors.initials: X
authors.lastname: Tao
authors.affiliation: Thoracic Department, Changzheng Hospital of Shanghai affiliated to The Secondary Military Medical University, Shanghai, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Thoracic Department, Changzheng Hospital of Shanghai affiliated to The Secondary Military Medical University, Shanghai, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Thoracic Department, Changzheng Hospital of Shanghai affiliated to The Secondary Military Medical University, Shanghai, China.
authors.forename: Tiewen
authors.initials: T
authors.lastname: Pan
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015820:Cadherins / Q000096:biosynthesis; D053148:Caspase 3 / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D019926:Cyclin B / Q000096:biosynthesis; D056741:Cyclin D / Q000096:biosynthesis; D019927:Cyclin E / Q000096:biosynthesis; D003839:Deoxyadenosines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D013997:Time Factors; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D015820:Cadherins; D019926:Cyclin B; D056741:Cyclin D; D019927:Cyclin E; D003839:Deoxyadenosines; D019253:Proto-Oncogene Proteins c-bcl-2; D014746:Vimentin; D053148:Caspase 3; D020780:Matrix Metalloproteinase 9; C058120:cordycepin
keywords: apoptosis cordycepin; lung cancer; migration; proliferation