title: MiR-125b regulates endometrial receptivity by targeting MMP26 in women undergoing IVF-ET with elevated progesterone on HCG priming day.
abstract: On the women undergoing IVF-ET with elevated progesterone on human chorionic gonadotrophin priming, the assisted reproductive technology outcome is poor. But, due to the unknown mechanism of this process, no effective method has been found to overcome this difficulty. Here, we investigated the roles of miR-125b and its target gene, MMP26, in endometrial receptivity (ER) in these women. The expression of miR-125b was significantly up-regulated in EECs in women with elevated progesterone during the window of implantation, and it showed a progesterone-dependent effect in vitro. Similarly, the expression of miR-125b was significantly up-regulated in the preimplantation period, and was down-regulated in the implantation period and the post-implantation period in mouse EECs. In addition, miR-125b showed a greater decrease at implantation sites than it did at interimplantation sites. The luciferase report assay demonstrated that MMP26 is a target gene of miR-125b. And the expression profile of MMP26 showed an inverse relationship with miR-125b in vivo and in vitro. Overexpression of miR-125b in human EECs inhibited cell migration and invasion. Gain-of-function of miR-125b induced a significant decrease in the number of implantation sites. In conclusion, these data shed new light on how miR-125b triggers ER decline through the regulation of MMP26 function.
authors.affiliation: Reproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Reproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Reproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Reproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Reproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, 100191, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000818:Animals; D004624:Embryo Transfer / Q000379:methods*; D004717:Endometrium / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D053505:Matrix Metalloproteinases, Secreted / Q000037:antagonists & inhibitors*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D011374:Progesterone / Q000008:administration & dosage*
chemical_list: C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D011374:Progesterone; C410599:MMP26 protein, human; D053505:Matrix Metalloproteinases, Secreted