title: Two Novel Relative Double-Stranded RNA Mycoviruses Infecting Fusarium poae Strain SX63.
abstract: Two novel double-stranded RNA (dsRNA) mycoviruses, termed Fusarium poae dsRNA virus 2 (FpV2) and Fusarium poae dsRNA virus 3 (FpV3), were isolated from the plant pathogenic fungus, Fusarium poae strain SX63, and molecularly characterized. FpV2 and FpV3, with respective genome sequences of 9518 and 9419 base pairs (bps), are both predicted to contain two discontinuous open reading frames (ORFs), ORF1 and ORF2. A hypothetical polypeptide (P1) and a RNA-dependent RNA polymerase (RdRp) are encoded by ORF1 and ORF2, respectively. Phytoreo_S7 domain (pfam07236) homologs were detected downstream of the RdRp domain (RdRp_4; pfam02123) of the ORF2-coded proteins of both FpV2 and FpV3. The same shifty heptamers (GGAAAAC) were both found immediately before the stop codon UAG of ORF1 in FpV2 and FpV3, which could mediate programmed -1 ribosomal frameshifting (-1 PRF). Phylogenetic analysis based on RdRp sequences clearly place FpV2 and FpV3 in a taxonomically unassigned dsRNA mycovirus group. Together, with a comparison of genome organization, a new taxonomic family termed Fusagraviridae is proposed to be created to include FpV2- and FpV3-related dsRNA mycoviruses, within which FpV2 and FpV3 would represent two distinct virus species.
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. wluan@iccas.ac.cn.
authors.forename: Luan
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. jingzezhang0820@gmail.com.
authors.forename: Jingze
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. zhanghailong.shenyang@gmail.com.
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. dewenqiu@caas.cn.
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
authors.affiliation: State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. guolihua@caas.cn.
authors.forename: Lihua
authors.initials: L
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000067148:Fungal Viruses / Q000145:classification / Q000235:genetics / Q000502:physiology*; D005670:Fusarium / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010455:Peptides / Q000235:genetics; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
chemical_list: D010455:Peptides; D012330:RNA, Double-Stranded; D012324:RNA-Dependent RNA Polymerase
keywords: FpV2; FpV3; Fusagraviridae; Fusarium poae; dsRNA virus; novel mycovirus