title: [Analysis on the nuclear division index of peripheral blood lymphocytes in the 281 general population of Anhui, China].
abstract: OBJECTIVE
  To investigate the reference range and influeing factors of the nuclear division index (NDI) of peripheral blood lymphocyte in Chinese general population in Anhui province.
  
  
  METHODS
  We selected 281 subjects from the general poulation in Anhui province, without occupational exposure to genetic toxicants and no chronic disease history. We used questionnaires to collect occupational history, age, gender, region, body mass index, smoking, and alcohol drinking status etc. NDI was measured by cytokinesis block micronucleus assay in peripheral blood lymphocytes, and the related factors were also analyzed. And NDI was used as the dependent variable, age, gender and other factors as independent variables to conduct stepwise multiple linear regression.
  
  
  RESULTS
  We found the data of NDI according with normal distribution, and the nuclear division index was 1.71±0.22, the minimum value was 1.10 while the maximum was 2.36. The results showed that NDI value of the males (1.67±0.20) were lower than that of the females (1.76±0.24), the difference was statistically significant (t=-3.65, P<0.001); current smokers NDI (1.66±0.18) lower than non-smokers (1.73±0.24) differences were statistically significant (t=3.06, P=0.002); the NDI of drinking groups (1.66±0.20) was lower than that of non-drinking population (1.74±0.23), the differences was statistically significant (t=3.15, P=0.002); Using multiple stepwise linear regression calibration factors and found that gender was an independent factor of NDI (β=0.098, Sx=0.027, t=3.66, P< 0.001).
  
  
  CONCLUSIONS
  We set the reference value on the nuclear division index among general population of survey areas in this study, it could provide a reference for similar studies and will provide reference for better evaluation of the effects of hazards on the body.
authors.affiliation: Key Lab of Chemical Safty and Health, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Teng
authors.forename: T J
authors.initials: TJ
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Ye
authors.forename: X Q
authors.initials: XQ
authors.lastname: Feng
authors.forename: Y X
authors.initials: YX
authors.lastname: Zheng
authors.forename: H W
authors.initials: HW
authors.lastname: Duan
mesh_terms: D000428:Alcohol Drinking; D048750:Cell Nucleus Division; D002681:China / Q000453:epidemiology; D048749:Cytokinesis / Q000187:drug effects*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology*; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D012016:Reference Values; D012907:Smoking / Q000453:epidemiology