title: Evaluation of the appropriateness of using glucometers for measuring the blood glucose levels in mice.
abstract: Glucometers are also widely used in diabetes research conducted using animal models. However, the appropriateness of measuring blood glucose levels using glucometers in animal models remains unclear. In this study, we evaluated the consistency between the blood glucose levels measured by 11 models of glucometers and plasma glucose levels measured by a laboratory biochemical test in blood samples collected by retro-orbital sinus puncture or tail-tip amputation. In both blood samples obtained by retro-orbital sinus puncture and those obtained by tail-tip amputation, 10 of the 11 models of glucometers yielded higher glucose values, while 1 yielded lower glucose values, than the plasma glucose values yielded by the laboratory test, the differences being in direct proportion to the plasma glucose values. Most glucometers recorded higher blood glucose levels after glucose loading and lower blood glucose levels after insulin loading in retro-orbital sinus blood as compared to tail vein blood. Our data suggest that the blood glucose levels measured by glucometers in mice tended to be higher than the plasma glucose levels yielded by the biochemical test under the hyperglycemic state, and that differences in the measured levels were observed according to the blood collection method depending on the glycemia status.
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Togashi
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Shirakawa
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Okuyama
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamazaki
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Kyohara
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Miyazawa
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Department of Endocrinology and Metabolism, Graduate school of Medicine Yokohama-City University, Yokohama 236-0004, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Terauchi
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D051379:Mice
chemical_list: D001786:Blood Glucose