title: Knockdown of ribosomal protein S15A induces human glioblastoma cell apoptosis.
abstract: BACKGROUND
  RPS15A is a ribosome protein that is highly conserved in many organisms from yeast to human. A number of studies implied its role in promoting cancer cell growth.
  
  
  METHODS
  Here, we firstly conducted RPS15A gene expression analysis in brain cancer using Oncomine database and found RPS15A was remarkably overexpressed in glioblastoma (GBM) compared with that in normal tissues. Then, the expression of RPS15A was specifically silenced in GBM cell line U251 using lentiviral-mediated RNA interference technique. We further investigated the effect of RPS15A knockdown in U251 cells using MTT assay, colony formation test, and flow cytometry analysis. We detected the protein level of Bcl-2 and poly (ADP-ribose) polymerase (PARP) as well as activation of caspase-3.
  
  
  RESULTS
  Our results showed that the knockdown of RPS15A could inhibit cancer cell growth and colony formation in vitro, as well as induced cell cycle arrest at G0/G1 phase and cell apoptosis. In addition, Western blot analysis indicated that the knockdown of RPS15A could significantly inhibit bcl-2 and activate caspase-3 and PARP.
  
  
  CONCLUSIONS
  Our findings suggest RPS15A may play an important role in the progression of GBM and lentiviral-mediated silencing of RPS15A could be an effective tool in GBM treatment.
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China.
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Fu
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
authors.affiliation: Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 274 Middle Zhijiang Road, Shanghai, 200071, China.
authors.forename: Bomi
authors.initials: B
authors.lastname: Ryu
authors.affiliation: College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
authors.forename: Shuili
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Sports and Health Research Center, Tongji University Department of Physical Education, Shanghai, 200092, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China.
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China. zhenglongpoy@163.com.
authors.forename: Longpo
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China. chenxianzheny@126.com.
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D049109:Cell Proliferation; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012269:Ribosomal Proteins; C074488:ribosomal protein S15a
keywords: Apoptosis; Cell proliferation; Glioblastoma; RPS15A