title: Quantitative analysis of vascular colonisation and angio-conduction in porous silicon-substituted hydroxyapatite with various pore shapes in a chick chorioallantoic membrane (CAM) model.
abstract: The development of scaffolds for bone filling of large defects requires an understanding of angiogenesis and vascular guidance, which are crucial processes for bone formation and healing. There are few investigations on the ability of a scaffold to support blood vessel guidance and it this is of great importance because it relates to the quality and dispersion of the blood vessel network. This work reports an analysis of vascularisation of porous silicon-substituted hydroxyapatite (SiHA) bioceramics and the effects of pore shape on vascular guidance using an expedient ex ovo model, the chick embryo chorioallantoic membrane (CAM) assay. Image analysis of vascularised implants assessed the vascular density, fractal dimension and diameter of blood vessels at two different scales (the whole ceramic and pores alone) and was performed on model SiHA ceramics harbouring pores of various cross-sectional geometries (circles, square, rhombus, triangles and stars). SiHA is a biocompatible material which allows the conduction of blood vessels on its surface. The presence of pores did not influence angiogenesis related-parameters (arborisation, fractal dimension) but pore geometry affected the blood vessel guidance and angio-conductive potential (diameter and number of the blood vessels converging toward the pores). The measured angles of pore cross-section modulated the number and diameter of blood vessels converging to pores, with triangular pores appearing of particular interest. This result will be used for shaping ceramic scaffolds with specific porous architecture to promote vascular colonisation and osteointegration.
  
  
  
  An expedient and efficient method, using chick embryo chorioallantoic membrane (CAM) assays, has been set up to characterise quantitatively the angiogenesis and the vascular conduction in scaffolds. This approach complements the usual cell culture assays and could replace to a certain extent in vivo experiments. It was applied to silicon-substituted hydroxyapatite porous bioceramics with various pore shapes. The material was found to be biocompatible, allowing the conduction of blood vessels on its surface. The presence of pores does not influence the angiogenesis but the pore shape affects the blood vessel guidance and angio-conductive potential. Pores with triangular cross-section appear particularly attractive for the further design of scaffolds in order to promote their vascular colonisation and osteointegration and improve their performances.
authors.affiliation: Univ. Limoges, CNRS, ENSCI, SPCTS UMR 7315, F-87000 Limoges, France; Univ. Limoges, EA3842 Homéostasie cellulaire et pathologies, F-87000 Limoges, France. Electronic address: amandine.magnaudeix@unilim.fr.
authors.forename: Amandine
authors.initials: A
authors.lastname: Magnaudeix
authors.affiliation: CHU Limoges, Service de Chirurgie Maxillo-Faciale, F-87000 Limoges, France. Electronic address: julieusseglio@hotmail.com.
authors.forename: Julie
authors.initials: J
authors.lastname: Usseglio
authors.affiliation: Univ. Limoges, CNRS, ENSCI, SPCTS UMR 7315, F-87000 Limoges, France. Electronic address: marie.lasgorceix@etu.unilim.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Lasgorceix
authors.affiliation: Univ. Limoges, EA3842 Homéostasie cellulaire et pathologies, F-87000 Limoges, France. Electronic address: fabrice.lalloue@unilim.fr.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Lalloue
authors.affiliation: Univ. Limoges, CNRS, ENSCI, SPCTS UMR 7315, F-87000 Limoges, France. Electronic address: chantal.damia@unilim.fr.
authors.forename: Chantal
authors.initials: C
authors.lastname: Damia
authors.affiliation: Univ. Limoges, CNRS, ENSCI, SPCTS UMR 7315, F-87000 Limoges, France; CHU Limoges, Service de Chirurgie Maxillo-Faciale, F-87000 Limoges, France. Electronic address: joel.brie@unilim.fr.
authors.forename: Joël
authors.initials: J
authors.lastname: Brie
authors.affiliation: Univ. Limoges, CNRS, ENSCI, SPCTS UMR 7315, F-87000 Limoges, France. Electronic address: patricia.pascaud-mathieu@unilim.fr.
authors.forename: Patricia
authors.initials: P
authors.lastname: Pascaud-Mathieu
authors.affiliation: Univ. Limoges, CNRS, ENSCI, SPCTS UMR 7315, F-87000 Limoges, France. Electronic address: eric.champion@unilim.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Champion
mesh_terms: D000818:Animals; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D016062:Porosity; D012825:Silicon / Q000737:chemistry / Q000494:pharmacology
chemical_list: D017886:Durapatite; D012825:Silicon
keywords: Chick chorioallantoic membrane; Microstereolithography; Porous ceramic; Silicon-substituted hydroxyapatite; Vascular colonisation