title: More insights into a human adipose tissue GPAT activity assay.
abstract: Adipose tissue fatty acid storage varies according to sex, adipose tissue depot and degree of fat gain. However, the mechanism(s) for these variations is not completely understood. We recently published findings based on the glycerol 3-phosphate acyltransferase (GPAT) enzyme activity assay we optimized for use with human adipose tissue. These findings include a decrease in total GPAT and GPAT1 as a function of adipocyte size in both omental and subcutaneous adipose tissue and a strong, positive correlations between ACS, GPAT, and DGAT activities for both sexes and depots and between these storage factors and palmitate storage rates into TAG. The aim of this commentary is to expand upon the data from our recent publication. We describe here additional details on the optimization of the GPAT enzyme activity assay, a correlation between DGAT and percentage palmitate in the diacylglycerol fraction, and sex differences in fatty acid storage factors and storage rates into TAG at high palmitate concentrations.
authors.affiliation: Endocrine Research Unit, Mayo Clinic ; Rochester, MN USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Morgan-Bathke
authors.affiliation: Endocrine Research Unit, Mayo Clinic; Rochester, MN USA; Guang'anmen Hospital; Xuan Wu District, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Endocrine Research Unit, Mayo Clinic; Rochester, MN USA; Department for Internal Medicine; Triemli Hospital; Zürich, Switzerland.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Oberschneider
authors.affiliation: Endocrine Research Unit, Mayo Clinic ; Rochester, MN USA.
authors.forename: Debra
authors.initials: D
authors.lastname: Harteneck
authors.affiliation: Endocrine Research Unit, Mayo Clinic ; Rochester, MN USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Jensen
keywords: DGAT; GPAT; fat distribution; fatty acid storage; omental adipose tissue; subcutaneous adipose tissue; triglyceride synthesis