title: Bisphenol A Impairs Mature Sperm Functions by a CatSper-Relevant Mechanism.
abstract: Bisphenol A (BPA), an endocrine-disrupting chemical, is widely used in the manufacture of daily necessities. Previous studies showed that BPA could impair spermatogenesis. However, its effects on mature spermatozoa are not well known. We aimed to investigate the in vivo and in vitro toxicity of BPA on mature mouse spermatozoa. Different doses of BPA (0, 10, 50, and 250 μg·kg(-1)·d(-1)) were administrated orally to C57BL/6 mice for 8 weeks. Subsequently, the sperm viability, motility, acrosome reaction (AR) ratio together with the expression/current levels of the sperm-specific Ca(2+ )channel (CatSper) and K(+ )channel (KSper) were examined. These parameters were also evaluated after applying BPA directly to normal mouse sperm to appraise the toxicity of BPA to mature sperm in vitro Significant decreases in sperm motility and AR were found in BPA administrated mice, possibly resulting from a BPA caused CatSper down-regulation which was supported by both western blot and patch clamping results. Moreover, direct application of BPA to spermatozoa inhibited CatSper transiently and also caused significant reductions in sperm total motility and AR ratio. In conclusion, both in vivo administration and in vitro application of BPA impair mature sperm functions by a CatSper-relevant mechanism.
authors.affiliation: Institute of Life Science, Nanchang University, Nanchang, Jiangxi, 330031, PR China.
authors.forename: Hua-Feng
authors.initials: HF
authors.lastname: Wang
authors.affiliation: Institute of Life Science, Nanchang University, Nanchang, Jiangxi, 330031, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Life Science, Nanchang University, Nanchang, Jiangxi, 330031, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Institute of Life Science, Nanchang University, Nanchang, Jiangxi, 330031, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: Institute of Life Science, Nanchang University, Nanchang, Jiangxi, 330031, PR China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zheng
authors.affiliation: Institute of Life Science, Nanchang University, Nanchang, Jiangxi, 330031, PR China zengxuhui@ncu.edu.cn.
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Zeng
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D015220:Calcium Channels / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D008297:Male; D008564:Membrane Potentials; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000633:toxicity*; D015398:Signal Transduction / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D015220:Calcium Channels; D052244:Endocrine Disruptors; D010636:Phenols; C488642:Catsper1 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; CatSper; acrosome reaction; endocrine disruptor.; sperm function