title: Syndapin promotes pseudocleavage furrow formation by actin organization in the syncytial Drosophila embryo.
abstract: Coordinated membrane and cytoskeletal remodeling activities are required for membrane extension in processes such as cytokinesis and syncytial nuclear division cycles in Drosophila Pseudocleavage furrow membranes in the syncytial Drosophila blastoderm embryo show rapid extension and retraction regulated by actin-remodeling proteins. The F-BAR domain protein Syndapin (Synd) is involved in membrane tubulation, endocytosis, and, uniquely, in F-actin stability. Here we report a role for Synd in actin-regulated pseudocleavage furrow formation. Synd localized to these furrows, and its loss resulted in short, disorganized furrows. Synd presence was important for the recruitment of the septin Peanut and distribution of Diaphanous and F-actin at furrows. Synd and Peanut were both absent in furrow-initiation mutants of RhoGEF2 and Diaphanous and in furrow-progression mutants of Anillin. Synd overexpression in rhogef2 mutants reversed its furrow-extension phenotypes, Peanut and Diaphanous recruitment, and F-actin organization. We conclude that Synd plays an important role in pseudocleavage furrow extension, and this role is also likely to be crucial in cleavage furrow formation during cell division.
authors.affiliation: Biology, Indian Institute of Science Education and Research, Pashan, Pune 411008, India.
authors.forename: Aparna
authors.initials: A
authors.lastname: Sherlekar
authors.affiliation: Biology, Indian Institute of Science Education and Research, Pashan, Pune 411008, India richa@iiserpune.ac.in.
authors.forename: Richa
authors.initials: R
authors.lastname: Rikhy
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D002970:Cleavage Stage, Ovum / Q000378:metabolism*; D003285:Contractile Proteins / Q000378:metabolism; D048749:Cytokinesis; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D004331:Drosophila melanogaster / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D015726:Giant Cells / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism
chemical_list: D000199:Actins; D002352:Carrier Proteins; D003285:Contractile Proteins; D029721:Drosophila Proteins; D008840:Microfilament Proteins; C540273:Synd protein, Drosophila; C095886:anillin