title: Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers.
abstract: Polydopamine, as an intermediate layer coated on PES membrane, was applied to fabricate various membrane adsorbers. Anion-exchange, hydrophobic interaction and affinity membrane adsorbers prepared by this facile method exhibited a high selectivity in fractionation of IgG (immunoglobulin)/HSA (human serum albumin) mixture. The anion-exchange membrane adsorber containing polyethylenimine (PEI) improved the HSA purity from 17.7% to 96.7%; The hydrophobic interaction membrane adsorber with Dodecyl mercaptan (DDM) as ligand obtained an IgG purity of 94.6%; Histidine attached affinity membrane chromatography achieved nearly a 100% purity of IgG. The present work indicated that the polydopamine layer not only activated membrane surface to attach various adsorptive ligands under the mild condition, but also reduced non-specific adsorption. Due to the versatile conjunction function, this facile mussel-inspired coating is also promising for the preparation of diverse membrane adsorbers.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: jqluo@ipe.ac.cn.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: yhwan@ipe.ac.cn.
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Wan
mesh_terms: D000327:Adsorption; D000838:Anions; D005591:Chemical Fractionation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000302:isolation & purification; D007211:Indoles / Q000737:chemistry*; D007474:Ion Exchange; D008024:Ligands; D008567:Membranes, Artificial; D011094:Polyethyleneimine; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012709:Serum Albumin / Q000302:isolation & purification; D013438:Sulfhydryl Compounds / Q000302:isolation & purification
chemical_list: D000838:Anions; D007074:Immunoglobulin G; D007211:Indoles; D008024:Ligands; D008567:Membranes, Artificial; D011108:Polymers; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C568283:polydopamine; C013976:dodecylmercaptan; D011094:Polyethyleneimine
keywords: Affinity membrane adsorber; Hydrophobic interaction; Ion-exchange; Membrane adsorber; Membrane chromatography