title: A glutathione S-transferase gene associated with antioxidant properties isolated from Apis cerana cerana.
abstract: Glutathione S-transferases (GSTs) are an important family of multifunctional enzymes in aerobic organisms. They play a crucial role in the detoxification of exogenous compounds, especially insecticides, and protection against oxidative stress. Most previous studies of GSTs in insects have largely focused on their role in insecticide resistance. Here, we isolated a theta class GST gene designated AccGSTT1 from Apis cerana cerana and aimed to explore its antioxidant and antibacterial attributes. Analyses of homology and phylogenetic relationships suggested that the predicted amino acid sequence of AccGSTT1 shares a high level of identity with the other hymenopteran GSTs and that it was conserved during evolution. Quantitative real-time PCR showed that AccGSTT1 is most highly expressed in adult stages and that the expression profile of this gene is significantly altered in response to various abiotic stresses. These results were confirmed using western blot analysis. Additionally, a disc diffusion assay showed that a recombinant AccGSTT1 protein may be roughly capable of inhibiting bacterial growth and that it reduces the resistance of Escherichia coli cells to multiple adverse stresses. Taken together, these data indicate that AccGSTT1 may play an important role in antioxidant processes under adverse stress conditions.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Shuchang
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China. xqguo@sdau.edu.cn.
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China. bhxu@sdau.edu.cn.
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism; D001516:Bees / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome
chemical_list: D000975:Antioxidants; D011994:Recombinant Proteins; D005982:Glutathione Transferase
keywords: Adverse stress; Antibacterial property; Antioxidant property; Apis cerana cerana; Glutathione S-transferase