title: The Long and Short of Circulating Cell-Free DNA and the Ins and Outs of Molecular Diagnostics.
abstract: The discovery of cell-free tumor and fetal DNA molecules in the plasma of cancer patients and pregnant women, respectively, has opened up exciting opportunities in molecular diagnosis. The understanding of the biological properties of circulating cell-free DNA (cfDNA) molecules would be essential for us to make the best use of such molecules in different clinical settings. In this review we start by exploring the technologies that have been used for analyzing the size profiles of cfDNA in plasma. We then review the size profiles of cfDNA in different clinical scenarios, including cancer, pregnancy, transplantation, and autoimmune diseases. Finally, we discuss the potential diagnostic applications of plasma DNA size profiling.
authors.affiliation: Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, China; Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, China; Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China. Electronic address: loym@cuhk.edu.hk.
authors.forename: Y M Dennis
authors.initials: YMD
authors.lastname: Lo
mesh_terms: D001327:Autoimmune Diseases / Q000097:blood* / Q000473:pathology; D004247:DNA / Q000097:blood; D004273:DNA, Neoplasm / Q000097:blood*; D005260:Female; D006801:Humans; D009369:Neoplasms / Q000097:blood* / Q000473:pathology; D057089:Pathology, Molecular; D011247:Pregnancy; D011296:Prenatal Diagnosis
chemical_list: D004273:DNA, Neoplasm; D004247:DNA
keywords: cell-free DNA; liquid biopsy; size profile