title: PREPARATION OF B-CYCLODEXTRIN-THIABENDAZOLE-PIPERONYL BUTOXIDE SUPRAMOLECULAR COMPLEX AND ITS ACTIVITY AGAINST BLUE AND GREEN MOULD DECAY ON INOCULATED SATSUMA FRUIT.
abstract: The supramolecular complex of β-cyclodextrin-thiabendazole-piperony butoxide (βCD-TBZ/PBO) was prepared and its structure was characterized by 1H NMR. Additionally, the antifungal activity of βCD-TBZ/PBO was investigated in comparison with the commercially available thiabendazole (TBZ) fungicide by in vitro tests and on artificially inoculated 'Okitsu' satsuma fruit dipped in water at 20 degrees C or at 50 degrees C to control postharvest blue (Penicillium italicum) and green mould (P. digitatum). β-CD-TBZ/PBO is stable for several months when stored as powder in a dark bottle. At pH 7.0 the water solubility of the βCD-TBZ/PBO complex was consistently higher than free TBZ. Water dip at 20 degrees C did not affect decay incidence caused by blue mould but favoured the development of green mould during 4-8 days of storage at 20 degrees C with respect to untreated (control) fruit. Water at 50 degrees C effectively reduced the incidence of blue mould and totally suppressed green mould during the first 4 days but lost its efficacy afterwards. By contrast, both TBZ and βCD-TBZ/PBO had a lasting effect and were equally effective in controlling green and blue mould decay when applied at 20 degrees C and 60 mg L(-1) active ingredient (a.i.). When applied at 50 degrees C and 20 mg L(-1) a.i. the activity of the complex against blue mould was inferior than the corresponding treatment with TBZ. In vitro assays revealed a significant effectiveness of βCD-TBZ/PBO complex at low concentration compared to commercial formulation of TBZ.
authors.forename: A
authors.initials: A
authors.lastname: Fadda
authors.forename: M
authors.initials: M
authors.lastname: Schirra
authors.forename: S
authors.initials: S
authors.lastname: D'Aquino
authors.forename: Q
authors.initials: Q
authors.lastname: Migheli
authors.forename: V
authors.initials: V
authors.lastname: Borzatta
authors.forename: G
authors.initials: G
authors.lastname: Delogu
mesh_terms: D002957:Citrus / Q000382:microbiology*; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology*; D061353:Food Storage; D005638:Fruit / Q000382:microbiology; D005659:Fungicides, Industrial / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010407:Penicillium / Q000187:drug effects* / Q000502:physiology; D010882:Piperonyl Butoxide / Q000737:chemistry / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D013827:Thiabendazole / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D003505:Cyclodextrins; D005659:Fungicides, Industrial; D010882:Piperonyl Butoxide; D013827:Thiabendazole