title: Physically crosslinked-sacran hydrogel films for wound dressing application.
abstract: The thin hydrogel films consisting of water-swollen polymer networks can potentially be applied for biomedical fields. Recently, natural polysaccharides have great attentions to be developed as wound healing and protection. In the present study, we newly prepared and characterized a physically crosslinked-hydrogel film composed of a novel megamolecular polysaccharide sacran for wound dressing application. We successfully fabricated a physically crosslinked-sacran hydrogel film by a solvent-casting method. The thickness of a sacran hydrogel film was lower than that of a sodium alginate (Na-alginate) film. Importantly, the swollen ratio of a sacran hydrogel film in water at 24h was 19-fold, compared to initial weight. Meanwhile, a Na-alginate hydrogel film was completely broken apart after rehydration. Moreover, a sacran hydrogel film did not show any cytotoxicity on NIH3T3 cells, a murine fibroblast cell line. The in vivo skin hydration study revealed that a sacran hydrogel film significantly increased the moisture content on hairless mice skin and considerably improved wound healing ability, compared to control (non-treated), probably due to not only the moisturing effect but also the anti-inflammatory effect of sacran. These results suggest that sacran has the potential properties as a basic biomaterial in a hydrogel film for wound dressing application.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan; Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
authors.forename: Nasrul
authors.initials: N
authors.lastname: Wathoni
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Motoyama
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Higashi
authors.affiliation: Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Noumi-shi, Ishikawa 923-1292, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Okajima
authors.affiliation: Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Noumi-shi, Ishikawa 923-1292, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan; Program for Leading Graduate Schools "HIGO (Health life science: Interdisciplinary and Glocal Oriented) Program", Kumamoto University, Japan. Electronic address: arimah@gpo.kumamoto-u.ac.jp.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arima
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000627:therapeutic use; D000818:Animals; D001458:Bandages; D020723:Glucuronic Acid / Q000737:chemistry / Q000627:therapeutic use; D006603:Hexuronic Acids / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000627:therapeutic use; D051379:Mice; D041681:NIH 3T3 Cells; D011134:Polysaccharides / Q000737:chemistry* / Q000627:therapeutic use; D014945:Wound Healing
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011134:Polysaccharides; C571099:sacran; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid
keywords: Hydrogel films; Sacran; Wound dressing