title: Monoclonal Antibody to Bone Marrow Stromal Cell Antigen 2 Protein of Swine.
abstract: The bone marrow stromal cell antigen 2 (BST-2) protein was identified as a novel virus restriction factor that potently restricts the replication and egress of enveloped viruses. In this study, we generated monoclonal antibodies (MAbs) against porcine BST-2 encoding 34-112 aa of porcine BST-2, which was cloned and inserted into the prokaryotic expression vector pCold-I to construct a recombinant plasmid pCold-pBST-2. The recombinant porcine BST-2 protein (rpBST-2 protein) was induced by isopropyl-β-D-thiogalactoside in Escherichia coli BL21 (DE3). Then, BALB/c mice were immunized with the purified rpBST-2 protein to prepare MAbs of BST-2. After subcloning, one strain of hybridoma cells named 1B2 secreting porcine BST-2 protein monoclonal antibody (MAb) was obtained. Indirect immunofluorescence assay and western blot analysis showed that the MAb was specifically reacted with the overexpressed porcine BST-2 protein in Vero cells. The specific MAb of porcine BST-2 provides a valuable tool for further studies of BST-2 to restrict virus infection.
authors.affiliation: 1 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University , Yangling, China .|2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .
authors.forename: Ning
authors.initials: N
authors.lastname: Kong
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .
authors.forename: Qiong
authors.initials: Q
authors.lastname: Meng
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .
authors.forename: Zhongze
authors.initials: Z
authors.lastname: Wang
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .
authors.forename: Yewen
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Wu
authors.initials: W
authors.lastname: Tong
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Guoxin
authors.initials: G
authors.lastname: Li
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Shen
authors.initials: S
authors.lastname: Yang
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Tongling
authors.initials: T
authors.lastname: Shan
authors.affiliation: 1 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University , Yangling, China .
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.affiliation: 2 Department of Swine Infectious Disease, Shanghai Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Shanghai, China .|3 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou, China .
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Tong
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D002522:Chlorocebus aethiops; D018352:Coronavirus Infections / Q000276:immunology* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D053485:Porcine epidemic diarrhea virus / Q000276:immunology / Q000472:pathogenicity; D011994:Recombinant Proteins; D013552:Swine / Q000276:immunology; D014709:Vero Cells
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D011994:Recombinant Proteins