title: Serum miRNAs Signature Plays an Important Role in Keloid Disease.
abstract: The molecular mechanism underlying the pathogenesis of keloid is largely unknown. MicroRNA (miRNA) is a class of small regulatory RNA that has emerged as a group of posttranscriptional gene repressors, participating in diverse pathophysiological processes of skin diseases. We investigated the expression profiles of miRNAs in the sera of patients to decipher the complicated factors involved in the development of keloid disease. MiRNA expression profiling in the sera from 9 keloid patients and 7 normal controls were characterized using a miRNA microarray containing established human mature and precursor miRNA sequences. Quantitative real-time PCR was performed to confirm the expression of miRNAs. The putative targets of differentially expressed miRNAs were functionally annotated by bioinformatics. MiRNA microarray analysis identified 37 differentially expressed miRNAs (17 upregulated and 20 downregulated) in keloid patients, compared to the healthy controls. Functional annotations revealed that the targets of those differentially expressed miRNAs were enriched in signaling pathways essential for scar formation and wound healing. The expression profiling of miRNAs is altered in the keloid, providing a clue for the molecular mechanisms underlying its initiation and progression. MiRNAs may partly contribute to the etiology of keloids by affecting the critical signaling pathways relevant to keloid pathogenesis.
authors.forename: Y
authors.initials: Y
authors.lastname: Luan
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Lin
authors.forename: F
authors.initials: F
authors.lastname: He
authors.affiliation: BRC1217C, Stanton L. Young Biomedical Research Center, 975 NE 10th St., Oklahoma City, OK 73104, USA. xiaoqun-dong@ouhsc.edu.
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Plastic and Aesthetic, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, People's Republic of China. xiaozhibodoctor@126.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D019295:Computational Biology / Q000379:methods; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007627:Keloid / Q000097:blood* / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D014945:Wound Healing / Q000235:genetics; D055815:Young Adult
chemical_list: D035683:MicroRNAs