title: Paenibacillus arachidis sp. nov., isolated from groundnut seeds.
abstract: A Gram-stain-positive, endospore-forming, rod-shaped, facultatively anaerobic bacterium, designated as strain E3T, was isolated from groundnut seeds. Based on the 16S rRNA gene sequence analysis, strain E3T belongs to the genus Paenibacillus with Paenibacillus thailandensis S3-4AT (96.0 %), Paenibacillus xanthinilyticus 11N27T (95.7 %), Paenibacillus mendelii C/2T (95.7 %) and other members of the genus Paenibacillus (<95.5 %) as its closest phylogenetic neighbours. The DNA G+C content of strain E3T was 53 mol%. Strain E3T was positive for gelatin hydrolysis, ammonification, catalase, chitinase production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, HCN production, siderophore production, biofilm formation, and urea and starch hydrolysis. Strain E3T had phosphatidylethanolamine, diphosphotidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, two unidentified aminolipids and two unidentified lipids as polar lipids. Strain E3T had diploptene, deplopterol and bacteriohopaneterol as major hopanoids. anteiso-C15 : 0 was the predominant cellular fatty acid with significant proportions of iso-C16 : 0, C16 : 0, C17 : 0, anteiso-C17 : 0, C18 : 1ω9c and iso-C14 : 0. Strain E3T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. On the basis of physiological, biochemical, chemotaxonomic and molecular analysis, strain E3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus arachidis sp. nov. is proposed. The type strain is E3T (=KCTC 33574T=LMG 28417T).
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
authors.forename: K
authors.initials: K
authors.lastname: Sadaf
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
authors.forename: L
authors.initials: L
authors.lastname: Tushar
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
authors.forename: P
authors.initials: P
authors.lastname: Nirosha
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
authors.forename: A R
authors.initials: AR
authors.lastname: Podile
authors.affiliation: Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad 500 085, India.
authors.forename: Ch
authors.initials: C
authors.lastname: Sasikala
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
authors.forename: Ch V
authors.initials: CV
authors.lastname: Ramana
mesh_terms: D010367:Arachis / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012639:Seeds / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid