title: The external morphology of adult female Egrasilus labracis as shown using hexamethyldisilazane treated, uncoated specimens for scanning electron microscopy.
abstract: The description and application of a modified Scanning Electron Microscope preparation technique using hexamethyldisilazane for small parasitic copepods was demonstrated though a high resolution depiction of individuals of Ergasilus labracis sampled from three spined stickleback (Gasterosteus aculeatus) in Bay D'Espoir, Newfoundland during summer 2015 and from archival samples retrieved from Atlantic salmon par (Salmo salar) stored at the Atlantic reference centre, St. Andrews, New Brunswick. The specimens were very well preserved showing high quality detail of important features and verifying those previously described using light microscopy by Hogans. Additionally the technique allowed excellent in situ demonstrations of mouth parts, swimming legs, and unusual and previously undescribed features of the second antenna including prominent striations and pore-like structures found to define the claw. It is thought that this technique will become a quick and efficient tool for describing important taxonomic features of small parasitic copepods like E. labracis or other similar small aquatic organisms. Microsc. Res. Tech. 79:657-663, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Fisheries and Oceans Canada, North Atlantic Fisheries Center, P.O. Box 5667, St John's, Newfoundland and Labrador, A1C 5X1, Canada.
authors.forename: Harry M
authors.initials: HM
authors.lastname: Murray
authors.affiliation: Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, A1C 5S7, Canada.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Hill
authors.affiliation: Cooke Aquaculture Inc, 669 Main Street, Blacks Harbour, New Brunswick, E5H 1K1, Canada.
authors.forename: Keng P
authors.initials: KP
authors.lastname: Ang
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000648:ultrastructure; D033342:Copepoda / Q000648:ultrastructure*; D005260:Female; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009055:Mouth / Q000648:ultrastructure; D017646:Organosilicon Compounds / Q000737:chemistry*
chemical_list: D017646:Organosilicon Compounds; C024548:hexamethylsilazane
keywords: HMDS; antennae; mandible; parasitic copepod; surface striation; swimming legs