title: Functional Self-Assembled Peptide Nanofibers for Bone Marrow Mesenchymal Stem Cell Encapsulation and Regeneration in Nucleus Pulposus.
abstract: Low back pain (LBP) is mainly caused by intervertebral disc degeneration (IDD). Recent studies have demonstrated that the transplantation of mesenchymal stem cells (MSCs) can regenerate regions that have undergone degeneration, and the regenerative effect can be enhanced by using a hydrogel carrier. This article describes an injectable functional hydrogel system manufactured by combining RADA16-I and RADA-KPSS (RADA-KPSS was manufactured by conjugating a bioactive motif derived from BMP-7 [KPSS] onto the C terminal of RADA16-I) at a volume ratio of 1:1. This hydrogel system can enhance the proliferation, differentiation, and chemotactic migration of BMSCs. In addition, the encapsulation of BMSCs with this system maintains cell viability for a long period after transplantation into an ex vivo cultured disc model. In conclusion, KPSS-conjugated RADKPS is an ideal encapsulation system for BMSCs in intervertebral disc (IVD) regeneration.
authors.affiliation: The Third Affiliated Hospital of Southern Medical University, Guangzhou.|Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Yaohong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.|Department of Orthopaedics, The 306th Hospital of People's Liberation Army, Beijing, People's Republic of China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Longgang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Hui
authors.initials: H
authors.lastname: Tao
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.affiliation: The Third Affiliated Hospital of Southern Medical University, Guangzhou.|Department of Orthopaedic Surgery, Navy General Hospital, Beijing.
authors.forename: Dike
authors.initials: D
authors.lastname: Ruan
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology; D007267:Injections; D055959:Intervertebral Disc Degeneration / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D057139:Nanofibers / Q000737:chemistry*; D000070614:Nucleus Pulposus / Q000166:cytology / Q000502:physiology*; D010455:Peptides / Q000737:chemistry*; D011817:Rabbits; D012038:Regeneration; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D055419:Bone Morphogenetic Protein 7; D010455:Peptides; C546681:RADA16-I
keywords: BMP-7; Bone marrow mesenchymal stem cells; Encapsulation; Functional self-assembling peptide; Intervertebral disc