title: Biotransformation of 5-hydroxy-methylfurfural into 2,5-furan-dicarboxylic acid by bacterial isolate using thermal acid algal hydrolysate.
abstract: Thermal acid hydrolysis is often used to deal with lignocellulosic biomasses, but 5-hydroxy-methylfurfural (5-HMF) formed during hydrolysis deeply influences downstream fermentation. 2,5-Furan-dicarboxylic acid (FDCA), which is in the list of future important biomass platform molecules can be obtained using 5-HMF biotransformation. Based on the connection between 5-HMF removal in acid hydrolysate and FDCA production, the optimum thermal acid hydrolysis condition for macroalgae Chaetomorpha linum was established. Potential microbes capable of transforming 5-HMF into FDCA were isolated and characterized under various parameters and inoculated into algal hydrolysate to perform 5-HMF biotransformation. The optimum hydrolysis condition was to apply 0.5M HCl to treat 3% algal biomass under 121°C for 15min. Isolated Burkholderia cepacia H-2 could transform 2000mg/L 5-HMF at the initial pH of 7 at 28°C and 1276mg/L FDCA was received. Strain B. cepacia H-2 was suitable for treating the algal hydrolysate without dilution, receiving 989.5mg/L FDCA.
authors.affiliation: Department of Safety Health and Environmental Engineering, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan. Electronic address: cfyang@yuntech.edu.tw.
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Yang
authors.affiliation: Department of Safety Health and Environmental Engineering, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan.
authors.forename: Ci-Ruei
authors.initials: CR
authors.lastname: Huang
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D016956:Burkholderia cepacia / Q000302:isolation & purification / Q000378:metabolism*; D000460:Chlorophyta / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000378:metabolism*; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005663:Furans / Q000378:metabolism*; D006868:Hydrolysis; D012624:Seaweed / Q000378:metabolism*
chemical_list: D003998:Dicarboxylic Acids; D005663:Furans; C008046:5-hydroxymethylfurfural; C551400:2,5-furandicarboxylic acid; D005662:Furaldehyde
keywords: 2,5-Furan-dicarboxylic acid (FDCA); 5-Hydroxy-methylfurfural (5-HMF); Biotransformation; Detoxification; Macroalgae biomass; Thermal acid hydrolysis