title: Mitochondrial Genomes of Anopheles (Kerteszia) (Diptera: Culicidae) From the Atlantic Forest, Brazil.
abstract: Mitochondrial genome sequences are widely used as molecular markers for phylogenetic studies of mosquito species complexes, such as the Anopheles albitarsis complex. Except for a few studies that employed a limited number of nuclear or mitochondrial loci to address the genetic structure and species status of Anopheles cruzii, Anopheles bellator, and Anopheles homunculus, little is known about genetic markers that can be employed in studies focusing on Kerteszia species. The complete mitochondrial genomes of seven specimens of An. bellator, An. cruzii, An. homunculus, and Anopheles laneanus were sequenced using long-range polymerase chain reaction and Illumina sequencing. The mitochondrial genomes varied from 15,446 to 15,738 bp in length and contained 37 genes (13 protein-encoding genes, 2 rRNA genes [12S rRNA and 16S rRNA] and 22 tRNA genes), and the AT-rich control region, as all do other Anopheles mitochondrial genomes sequenced to date. Specimens from four populations of An. cruzii showed differences in codon composition.
authors.affiliation: Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, Ave. Doutor Arnaldo 715, 01246-904 São Paulo, SP, Brazil (porangaba@usp.br; sayuri20@uol.com.br; masallum@usp.br), masallum@usp.br.
authors.forename: T M P
authors.initials: TM
authors.lastname: Oliveira
authors.affiliation: Department of Life Sciences, Natural History Museum, Cromwell Rd., London, UK (p.foster2@gmail.com).
authors.forename: P G
authors.initials: PG
authors.lastname: Foster
authors.affiliation: Superintendência de Controle de Endemias, Secretaria de Estado da Saúde de São Paulo, R. Rui Barbosa, 1672, 14810-095 Araraquara, SP, Brazil (edusteber@uol.com.br).
authors.forename: E S
authors.initials: ES
authors.lastname: Bergo
authors.affiliation: Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, Ave. Doutor Arnaldo 715, 01246-904 São Paulo, SP, Brazil (porangaba@usp.br; sayuri20@uol.com.br; masallum@usp.br).
authors.forename: S S
authors.initials: SS
authors.lastname: Nagaki
authors.affiliation: Departamento de Patologia, LIM 03, Hospital das Clinicas (HC), Escola de Medicina, Universidade of São Paulo, Ave. Dr. Enéas de Carvalho Aguiar, 05403-000 São Paulo, Brazil (sabyem_63@yahoo.com).
authors.forename: S S
authors.initials: SS
authors.lastname: Sanabani
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California Irvine, 2315 McGaugh Hall, Irvine, CA 92697 (omarinotti@gmail.com; pedromarinotti@gmail.com), and.
authors.forename: O
authors.initials: O
authors.lastname: Marinotti
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California Irvine, 2315 McGaugh Hall, Irvine, CA 92697 (omarinotti@gmail.com; pedromarinotti@gmail.com), and.
authors.forename: P N
authors.initials: PN
authors.lastname: Marinotti
authors.affiliation: Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, Ave. Doutor Arnaldo 715, 01246-904 São Paulo, SP, Brazil (porangaba@usp.br; sayuri20@uol.com.br; masallum@usp.br), masallum@usp.br.
authors.forename: M A M
authors.initials: MA
authors.lastname: Sallum
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001938:Brazil; D005260:Female; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D008297:Male; D017422:Sequence Analysis, DNA
keywords: Anopheles bellator; Anopheles cruzii; Anopheles homunculus; Anopheles laneanus; mitochondrial genome