title: Ruminant and industrial trans-fatty acid uptake in the heart.
abstract: Dietary trans-fats are strongly associated with heart disease. However, the capacity for the tissues of the body, and specifically the heart, to take up trans-fats is unknown. It is also unknown if different trans-fats have different uptake capacities in the heart and other tissues of the body. Diets of low-density lipoprotein receptor-deficient mice were supplemented for 14weeks with foods that contained 1.5% of the trans-fat elaidic acid or vaccenic acid. Tissues were extracted and frozen in liquid nitrogen, and then lipids were analyzed by gas chromatography for fatty acid content. Isolated cardiomyocytes were also exposed to elaidic or vaccenic acid in cell culture media for 24h. Dietary supplementation with vaccenic or elaidic acid resulted in a 20-fold higher accumulation of these TFAs in fat deposits in the body in comparison to liver. Liver tissue accumulated about twice as much per gram tissue as heart. Similar quantities of both elaidic acid and vaccenic acid were taken up by the tissues. Isolated cardiomyocytes exhibited an unusually large uptake of trans-fat, and this was dependent upon both the concentration and duration of exposure to the trans-fats but not upon the type of trans-fat. Expression levels of CD36 and FATP4 were not significantly changed during dietary interventions or exposure of cells to trans-fats. We conclude that fat, liver and heart (including cardiomyocytes) are all capable of accumulating trans-fat in response to dietary supplementation without changes in fatty acid transport protein expression.
authors.affiliation: Canadian Centre for Agri-food Research in Health and Medicine, St Boniface Hospital and the Institute of Cardiovascular Sciences, Department of Physiology and Pathophysiology, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: Riya
authors.initials: R
authors.lastname: Ganguly
authors.affiliation: Canadian Centre for Agri-food Research in Health and Medicine, St Boniface Hospital and the Institute of Cardiovascular Sciences, Department of Physiology and Pathophysiology, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: Renee
authors.initials: R
authors.lastname: LaVallee
authors.affiliation: Canadian Centre for Agri-food Research in Health and Medicine, St Boniface Hospital and the Institute of Cardiovascular Sciences, Department of Physiology and Pathophysiology, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: Thane G
authors.initials: TG
authors.lastname: Maddaford
authors.affiliation: Canadian Centre for Agri-food Research in Health and Medicine, St Boniface Hospital and the Institute of Cardiovascular Sciences, Department of Physiology and Pathophysiology, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: Brittany
authors.initials: B
authors.lastname: Devaney
authors.affiliation: Canadian Centre for Agri-food Research in Health and Medicine, St Boniface Hospital and the Institute of Cardiovascular Sciences, Department of Physiology and Pathophysiology, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: Chantal M C
authors.initials: CM
authors.lastname: Bassett
authors.affiliation: Canadian Centre for Agri-food Research in Health and Medicine, St Boniface Hospital and the Institute of Cardiovascular Sciences, Department of Physiology and Pathophysiology, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Edel
authors.affiliation: Canadian Centre for Agri-food Research in Health and Medicine, St Boniface Hospital and the Institute of Cardiovascular Sciences, Department of Physiology and Pathophysiology, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada. Electronic address: gpierce@sbrc.ca.
authors.forename: Grant N
authors.initials: GN
authors.lastname: Pierce
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D003470:Culture Media; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics; D012418:Ruminants; D044242:Trans Fatty Acids / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011973:Receptors, LDL; D044242:Trans Fatty Acids
keywords: Cardiovascular disease; Diet; Fatty acid; Heart; Lipid transport; Nutrition; Trans-fat