title: Association of nineteen polymorphisms from seven DNA repair genes and the risk for bladder cancer in Gansu province of China.
abstract: BACKGROUND
  Balance of DNA damage and proper repair plays an important role in progression of bladder cancer. Here we aimed to assess the associations of nineteen polymorphisms from seven DNA repair-associated genes (PRAP1, OGG1, APEX1, MUTYH, XRCC1, XRCC2 and XRCC3) with bladder cancer and their interactions in the disease in a Han Chinese population.
  
  
  RESULTS
  A chip-based TaqMan genotyping for the candidate genes was performed on 227 bladder cancer patients and 260 healthy controls. APEX1 rs3136817, MUTYH rs3219493, three SNPs (rs3213356, rs25487 and rs1799782) in XRCC1, and three SNPs (rs1799794, rs861531 and rs861530) in XRCC3 showed significant associations with the risk of bladder cancer. In haplotype analysis, elevated risks of bladder cancer were observed in those with either haplotype GT (OR = 1.56, P = 0.003) of APEX1, or GGGTC (OR = 2.05, P = 0.002) of XRCC1, whereas decreased risks were in individuals with either GCGCC (OR = 0.40, P = 0.001), or GCGTT (OR = 0.60, = 0.005) of XRCC1, or CCC (OR = 0.65, P = 0.004) of MUTYH, or TTTAT (OR = 0.36, P = 0.009) of XRCC3. Interaction analysis showed that the two-loci model (rs1799794 and rs861530) was the best with the maximal testing accuracy of 0.701, and the maximal 100% cross-validation consistency (P = 0.001).
  
  
  CONCLUSIONS
  Polymorphisms and haplotypes of DNA repair genes are associated with the risk of bladder cancer, and of which the SNPs (rs1799794 and rs861530) in XRCC3 gene might be two major loci in relation to the susceptibility to bladder cancer in a northwest Chinese population.
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China.|Gansu Provincial Academy of Medical Sciences, Gansu Provincial Cancer Hospital, Lanzhou, Gansu 730050, China.
authors.forename: Gongjian
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Gansu Provincial Academy of Medical Sciences, Gansu Provincial Cancer Hospital, Lanzhou, Gansu 730050, China.
authors.forename: Haixiang
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Chronic Disease Epidemiology, Yale School of Public Health, School of Medicine, Yale Cancer Center, Yale University, New Haven, CT 06520-8034, USA.
authors.forename: Lingeng
authors.initials: L
authors.lastname: Lu
authors.affiliation: Gansu Provincial Academy of Medical Sciences, Gansu Provincial Cancer Hospital, Lanzhou, Gansu 730050, China.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Guo
authors.affiliation: Institute of Urology, the Second Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Institute of Urology, the Second Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Song
authors.affiliation: Department of Internal Medicine, Xigu District of Lanzhou City People's Hospital, Lanzhou, Gansu 730050, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Gansu Provincial Academy of Medical Sciences, Gansu Provincial Cancer Hospital, Lanzhou, Gansu 730050, China.
authors.forename: Haining
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Urology, the Second Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D004260:DNA Repair / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D001749:Urinary Bladder Neoplasms / Q000208:ethnology / Q000235:genetics*
keywords: DNA repair; bladder cancer; gene interaction; polymorphisms