title: Involvement of 3D osteoblast migration and bone apatite during in vitro early osteocytogenesis.
abstract: The transition from osteoblast to osteocyte is described to occur through passive entrapment mechanism (self-buried, or embedded by neighboring cells). Here, we provide evidence of a new pathway where osteoblasts are "more" active than generally assumed. We demonstrate that osteoblasts possess the ability to migrate and differentiate into early osteocytes inside dense collagen matrices. This step involves MMP-13 simultaneously with IBSP and DMP1 expression. We also show that osteoblast migration is enhanced by the presence of apatite bone mineral. To reach this conclusion, we used an in vitro hybrid model based on both the structural characteristics of the osteoid tissue (including its density, texture and three-dimensional order), and the use of bone-like apatite. This finding highlights the mutual dynamic influence of osteoblast cell and bone extra cellular matrix. Such interactivity extends the role of physicochemical effects in bone morphogenesis complementing the widely studied molecular signals. This result represents a conceptual advancement in the fundamental understanding of bone formation.
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Robin
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Claudia
authors.initials: C
authors.lastname: Almeida
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Azaïs
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Haye
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Illoul
authors.affiliation: EA 2496, Pathologies, Imaging and Biotherapies of the Tooth, UFR Odontologie, University Paris Descartes PRES Sorbonne Paris Cite, Montrouge, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Lesieur
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France.
authors.forename: Marie-Madeleine
authors.initials: MM
authors.lastname: Giraud-Guille
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France. Electronic address: nadine.nassif@upmc.fr.
authors.forename: Nadine
authors.initials: N
authors.lastname: Nassif
authors.affiliation: Sorbonne Universités UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire Chimie de la Matière Condensée de Paris UMR 7574, 11 place Marcelin Berthelot, 75005 Paris, France. Electronic address: christophe.helary@upmc.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hélary
mesh_terms: D000818:Animals; D001031:Apatites / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology*; D010012:Osteogenesis; D010641:Phenotype; D051381:Rats; D012756:Sheep
chemical_list: D001031:Apatites
keywords: Bone apatite; Migration; Nanotopography; Osteoblast; Osteocyte differentiation