title: Quantitation of Vacuolar Sugar Transporter Abundance Changes Using QconCAT Synthtetic Peptides.
abstract: Measurements of protein abundance changes are important for biological conclusions on protein-related processes such as activity or complex formation. Proteomic analyses in general are almost routine tasks in many laboratories, but a precise and quantitative description of (absolute) protein abundance changes require careful experimental design and precise data quality. Today, a vast choice of metabolic labeling and label-free quantitation protocols are available, but the trade-off between quantitative precision and proteome coverage of quantified proteins including missing value problems remain. Here, we provide an example of a targeted proteomic approach using artificial standard proteins consisting of concatenated peptides of interest (QconCAT) to specifically quantify abiotic stress-induced abundance changes in low abundant vacuolar transporters. An advantage of this approach is the reliable quantitation of alimited set of low-abundant target proteins throughout different conditions. We show that vacuolar ATPase AVP1 and sugar transporters of the ERDL (early responsive to dehydration-like) family and TMT2 (tonoplast monosaccharide transporter 2) showed increased abundance upon salt stress.
authors.affiliation: Department of Plant Systems Biology, University of Hohenheim Stuttgart, Germany.
authors.forename: Heidi
authors.initials: H
authors.lastname: Pertl-Obermeyer
authors.affiliation: Plant Physiology, University of Kaiserslautern Kaiserslautern, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Trentmann
authors.affiliation: Plant Physiology, University of Kaiserslautern Kaiserslautern, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Duscha
authors.affiliation: Plant Physiology, University of Kaiserslautern Kaiserslautern, Germany.
authors.forename: H Ekkehard
authors.initials: HE
authors.lastname: Neuhaus
authors.affiliation: Department of Plant Systems Biology, University of Hohenheim Stuttgart, Germany.
authors.forename: Waltraud X
authors.initials: WX
authors.lastname: Schulze
keywords: QconCATs; drought stress; salt stress; sugar transport in plants; vacuole