title: Isoniazid hair concentrations in children with tuberculosis: a proof of concept study.
abstract: Assessing treatment adherence and quantifying exposure to anti-tuberculosis drugs among children is challenging. We undertook a 'proof of concept' study to assess the drug concentrations of isoniazid (INH) in hair as a therapeutic drug monitoring tool. Children aged <12 years initiated on a thrice-weekly treatment regimen including INH (10 mg/kg) for newly diagnosed tuberculosis were enrolled. INH concentrations in hair were measured using liquid chromatography-tandem mass spectrometry at 1, 2, 4 and 6 months after initiating anti-tuberculosis treatment. We found that INH hair concentrations in all children on thrice-weekly INH were detectable and displayed variability across a dynamic range.
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India; Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: V
authors.initials: V
authors.lastname: Mave
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: A
authors.initials: A
authors.lastname: Chandanwale
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: A
authors.initials: A
authors.lastname: Kinikar
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Khadse
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: A
authors.initials: A
authors.lastname: Kagal
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India; Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: N
authors.initials: N
authors.lastname: Gupte
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: N
authors.initials: N
authors.lastname: Suryavanshi
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Nimkar
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: H
authors.initials: H
authors.lastname: Koli
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Khwaja
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: R
authors.initials: R
authors.lastname: Bharadwaj
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Joshi
authors.affiliation: University of California, San Francisco, California, USA.
authors.forename: H
authors.initials: H
authors.lastname: Horng
authors.affiliation: University of California, San Francisco, California, USA.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Benet
authors.affiliation: National Institute of Research in Tuberculosis, Chennai, India.
authors.forename: G
authors.initials: G
authors.lastname: Ramachandran
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Dooley
authors.affiliation: Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India; Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: A
authors.initials: A
authors.lastname: Gupta
authors.affiliation: University of California, San Francisco, California, USA.
authors.forename: M
authors.initials: M
authors.lastname: Gandhi
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis* / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid; D016903:Drug Monitoring; D005260:Female; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007538:Isoniazid / Q000032:analysis* / Q000627:therapeutic use; D008137:Longitudinal Studies; D008297:Male; D010349:Patient Compliance; D000075082:Proof of Concept Study; D011446:Prospective Studies; D053719:Tandem Mass Spectrometry; D014376:Tuberculosis / Q000175:diagnosis / Q000188:drug therapy*
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid