title: Oxidative Profile and δ-Aminolevulinate Dehydratase Activity in Healthy Pregnant Women with Iron Supplementation.
abstract: An oxidative burst occurs during pregnancy due to the large consumption of oxygen in the tissues and an increase in metabolic demands in response to maternal physiological changes and fetal growth. This study aimed to determine the oxidative profile and activity of δ-aminolevulinate dehydratase (δ-ALA-D) in pregnant women who received iron supplementation. Oxidative stress parameters were evaluated in 25 pregnant women with iron supplementation, 25 pregnant women without supplementation and 25 non-pregnant women. The following oxidative stress parameters were evaluated: thiobarbituric acid reactive substances (TBARS), protein thiol groups (P-SH), non-protein thiol levels (NP-SH), vitamin C levels, catalase and δ-ALA-D activity. Markers of oxidative stress and cell damage, such as TBARS in plasma were significantly higher in pregnant women without supplementation. Levels of P-SH, NP-SH and δ-ALA-D activity were significantly lower in pregnant women without supplementation compared to non-pregnant and pregnant women with supplementation, while vitamin C levels were significantly lower in pregnant women without supplementation when compared to non-pregnant women. The increase in the generation of oxidative species and decrease of antioxidants suggest the loss of physiological oxidative balance during normal pregnancy, which was not observed in pregnant women with iron supplementation, suggesting a protective effect of iron against oxidative damage.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, Brazil. leidi_lucca@hotmail.com.
authors.forename: Leidiane
authors.initials: L
authors.lastname: De Lucca
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, Brazil. fabianeufsm@gmail.com.
authors.forename: Fabiane
authors.initials: F
authors.lastname: Rodrigues
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, Brazil. letii_jantsch@hotmail.com.
authors.forename: Letícia B
authors.initials: LB
authors.lastname: Jantsch
authors.affiliation: Departamet of Obstetrics and Gynecology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, Brazil. wsneme@yahoo.com.br.
authors.forename: Walter S
authors.initials: WS
authors.lastname: Neme
authors.affiliation: Departamet of Obstetrics and Gynecology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, Brazil. fmgallarreta@msn.com.
authors.forename: Francisco M P
authors.initials: FM
authors.lastname: Gallarreta
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, Brazil. thissianegoncalves@yahoo.com.br.
authors.forename: Thissiane L
authors.initials: TL
authors.lastname: Gonçalves
mesh_terms: D000328:Adult; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D006801:Humans; D007501:Iron / Q000008:administration & dosage / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D011247:Pregnancy; D055815:Young Adult
chemical_list: D007501:Iron; D000623:Porphobilinogen Synthase
keywords: antioxidant; iron; oxidative stress; pregnant women; δ-aminolevulinate dehydratase