title: Differentiation Potential of Mesenchymal Stem Cells from Equine Bone Marrow Cultured on Hyaluronic Acid-Chitosan Polyelectrolyte Multilayer Biofilm.
abstract: Nanotechnology techniques have a prominent role in the current technical and scientific scene. The layer-by-layer (LbL) deposition allows obtaining nanostructures with sophisticated multilayer, using a simple, but versatile technique. This procedure, which is used to coat and functionalize surfaces with nanometer- thick films, has applications in bioengineering, medicine, chemistry, materials and chemical engineering among other areas. Chitosan is a biomaterial, coming from the chitin, a very abundant polymer in nature, which has been recently tested as scaffolds. In this experiment we test the hypothesis that the hyaluronic acid-chitosan polyelectrolyte multilayer biofilm would be a good substrate to the adherence of equine mesenchymal stem cells derived from bone marrow. The results showed that these biofilms accelerate the process of cell adhesion on smooth surfaces, allowing a constant cell growth and creating a great option to cover surgical materials.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Listoni
authors.forename: Isadora
authors.initials: I
authors.lastname: Arruda
authors.forename: Leandro
authors.initials: L
authors.lastname: Maia
authors.forename: Danielle J
authors.initials: DJ
authors.lastname: Barberini
authors.forename: Ian
authors.initials: I
authors.lastname: Martins
authors.forename: Fernando C
authors.initials: FC
authors.lastname: Vasconcellos
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Landim-Alvarenga
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D006736:Horses; D006820:Hyaluronic Acid / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D011107:Polylysine / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D008567:Membranes, Artificial; D011107:Polylysine; D006820:Hyaluronic Acid; D048271:Chitosan