title: PCNA is involved in the EndoQ-mediated DNA repair process in Thermococcales.
abstract: To maintain genome integrity for transfer to their offspring, and to maintain order in cellular processes, all living organisms have DNA repair systems. Besides the well-conserved DNA repair machineries, organisms thriving in extreme environments are expected to have developed efficient repair systems. We recently discovered a novel endonuclease, which cleaves the 5' side of deoxyinosine, from the hyperthermophilic archaeon, Pyrococcus furiosus. The novel endonuclease, designated as Endonulcease Q (EndoQ), recognizes uracil, abasic site and xanthine, as well as hypoxanthine, and cuts the phosphodiester bond at their 5' sides. To understand the functional process involving EndoQ, we searched for interacting partners of EndoQ and identified Proliferating Cell Nuclear Angigen (PCNA). The EndoQ activity was clearly enhanced by addition of PCNA in vitro. The physical interaction between the two proteins through a PIP-motif of EndoQ and the toroidal structure of PCNA are critical for the stimulation of the endonuclease activity. These findings provide us a clue to elucidate a unique DNA repair system in Archaea.
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.|Institute for Universal Biology and University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.|Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Miyako
authors.initials: M
authors.lastname: Shiraishi
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Sonoko
authors.initials: S
authors.lastname: Ishino
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamagami
authors.affiliation: Institute for Universal Biology and University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.|Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.|Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.|Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Isaac
authors.initials: I
authors.lastname: Cann
authors.affiliation: Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.|Institute for Universal Biology and University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.|Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Yoshizumi
authors.initials: Y
authors.lastname: Ishino
mesh_terms: D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D025941:Protein Interaction Mapping; D020027:Pyrococcus furiosus / Q000201:enzymology* / Q000235:genetics
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D004706:Endodeoxyribonucleases