title: Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ.
abstract: Both Tembusu virus (TMUV) and goose parvovirus (GPV) are causative agents of goose disease. However, the host immune response of the goose against these two different categories of virus has not been well documented. Here, we compared the clinical symptoms and pathological characteristics, antigen distribution and intensity, and expression of immune-related genes in TMUV- and GPV- infected goose. The immunohistochemistry analysis demonstrated that GPV was primarily located in the liver, lung, small intestine, and rectum, while TMUV was situated in the liver, brain, spleen, and small intestine. The induction of IFNγ and proinflammatory cytokines is highly associated with the distribution profiles of antigen and CD8α+ molecules. The effector function of CD8 T cells may be accomplished by the secretion of IFNγ together with high expression of proinflammatory cytokines such as IL1 and IL6. Remarkably, significant increases in the transcription of immune genes were observed after infection, which suggested that both GPV and TMUV can effectively induce immune response in goose PMBCs. This study will provide fundamental information for goose molecular immunology in defending against pandemic viruses.
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Mingshu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.affiliation: Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Dekang
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Mafeng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Kunfeng
authors.initials: K
authors.lastname: Sun
authors.affiliation: Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jing
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Anchun
authors.initials: A
authors.lastname: Cheng
mesh_terms: D000825:Animal Structures / Q000821:virology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005416:Flavivirus / Q000276:immunology*; D018177:Flavivirus Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D005777:Geese; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000378:metabolism; D010322:Parvoviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D019219:Parvovirinae / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D007371:Interferon-gamma