title: Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds.
abstract: In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC50 = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC50 = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells.
authors.affiliation: Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka, Miyazaki 882-8508, Japan. hkai@phoenix.ac.jp.|Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan. hkai@phoenix.ac.jp.
authors.forename: Hisahiro
authors.initials: H
authors.lastname: Kai
authors.affiliation: Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan. rojopino@mac.com.
authors.forename: Ena
authors.initials: E
authors.lastname: Akamatsu
authors.affiliation: Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan. ushio16@fc.miyazaki-u.ac.jp.
authors.forename: Eri
authors.initials: E
authors.lastname: Torii
authors.affiliation: Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan. yukizaki@iri.pref.miyazaki.jp.|Miyazaki Prefectural Food Research and Development Center, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan. yukizaki@iri.pref.miyazaki.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kodama
authors.affiliation: Miyazaki Prefectural Food Research and Development Center, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan. yukizaki@iri.pref.miyazaki.jp.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Yukizaki
authors.affiliation: Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan. akagi046@chem.agri.kagoshima-u.ac.jp.|Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan. akagi046@chem.agri.kagoshima-u.ac.jp.
authors.forename: Isao
authors.initials: I
authors.lastname: Akagi
authors.affiliation: Miyazaki Agricultural Experiment Station, 5805 Shimonaka, Sadowara-cho, Miyazaki, Miyazaki 880-0212, Japan. ino-hisatoshi@pref.miyazaki.lg.jp.
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Ino
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, Miyazaki 889-2192, Japan. ysakaki@cc.miyazaki-u.ac.jp.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakakibara
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, Miyazaki 889-2192, Japan. msuiko@cc.miyazaki-u.ac.jp.
authors.forename: Masahito
authors.initials: M
authors.lastname: Suiko
authors.affiliation: Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan. yamamoto@fc.miyazaki-u.ac.jp.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Yamamoto
authors.affiliation: Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan. okayama@med.miyazaki-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okayama
authors.affiliation: Division of Tumor and Cellular Biochemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan. kmorishi@fc.miyazaki-u.ac.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Morishita
authors.affiliation: Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan. mejina@med.miyazaki-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kataoka
authors.affiliation: Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka, Miyazaki 882-8508, Japan. kjmtsn@phoenix.ac.jp.
authors.forename: Koji
authors.initials: K
authors.lastname: Matsuno
keywords: adult T-cell leukemia; bitter gourd seed extract; phytohemagglutinin-activated human peripheral blood mononuclear cell; α-eleostearic acid