title: The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types.
abstract: Skeletal muscle satellite cells are a muscle stem cell population that mediate posthatch muscle growth and repair. Satellite cells respond differentially to environmental stimuli based upon their fiber-type of origin. The objective of this study was to determine how temperatures below and above the in vitro control of 38°C affected the proliferation and differentiation of satellite cells isolated from the chicken anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b.femoris) muscles. The satellite cells isolated from the p. major muscle were more sensitive to both cold and hot temperatures compared to the b.femoris satellite cells during both proliferation and differentiation. The expressions of myogenic regulatory transcription factors were also different between satellite cells from different fiber types. MyoD expression, which partially regulates proliferation, was generally expressed at higher levels in p. major satellite cells compared to the b.femoris satellite cells from 33 to 43°C during proliferation and differentiation. Similarly, myogenin expression, which is required for differentiation, was also expressed at higher levels in p. major satellite cells in response to both cold and hot temperatures during proliferation and differentiation than b. femoris satellite cells. These data demonstrate that satellite cells from the anaerobic p. major muscle are more sensitive than satellite cells from the aerobic b. femoris muscle to both hot and cold thermal stress during myogenic proliferation and differentiation.
authors.affiliation: Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio.
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Harding
authors.affiliation: Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.
authors.forename: Orna
authors.initials: O
authors.lastname: Halevy
authors.affiliation: Institute of Animal Sciences, Agricultural Research Organization The Volcani Center, Bet Dagan, Israel.
authors.forename: Shlomo
authors.initials: S
authors.lastname: Yahav
authors.affiliation: Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio Velleman.1@osu.edu.
authors.forename: Sandra G
authors.initials: SG
authors.lastname: Velleman
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D002645:Chickens / Q000502:physiology*; D018006:Myogenic Regulatory Factors / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*; D013696:Temperature; D059467:Transcriptome
chemical_list: D018006:Myogenic Regulatory Factors
keywords: Chicken; fiber type; muscle; satellite cells; temperature