title: Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum.
abstract: Prp28 (pre-mRNA-splicing ATP-dependent RNA helicase 28) is a spliceosomal DEAD-box helicase which is involved in two steps of spliceosome assembly. It is required for the formation of commitment complex 2 in an ATP-independent manner as well as for the formation of the pre-catalytic spliceosome, which in contrast is ATP-dependent. During the latter step, Prp28 is crucial for the integration of the U4/U6·U5 tri-snRNP since it displaces the U1 snRNP and allows the U6 snRNP to base-pair with the 5'-splice site. Here, the crystal structure of Prp28 from the thermophilic fungus Chaetomium thermophilum is reported at 3.2 Å resolution and is compared with the available structures of homologues.
authors.affiliation: Department of Molecular Structural Biology, Institute for Microbiology and Genetics, GZMB, Georg-August-University Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
authors.forename: Marcel J
authors.initials: MJ
authors.lastname: Tauchert
authors.affiliation: Department of Molecular Structural Biology, Institute for Microbiology and Genetics, GZMB, Georg-August-University Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Ficner
mesh_terms: D002597:Chaetomium / Q000201:enzymology*; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D020365:RNA Helicases / Q000737:chemistry*; D017381:Spliceosomes / Q000201:enzymology*
chemical_list: D020365:RNA Helicases
keywords: DDX23; DEAD-box protein; RNA helicase; U5-100kD; spliceosome