title: Proteogenomic Characterization of Monocyclic Aromatic Hydrocarbon Degradation Pathways in the Aniline-Degrading Bacterium Burkholderia sp. K24.
abstract: Burkholderia sp. K24, formerly known as Acinetobacter lwoffii K24, is a soil bacterium capable of utilizing aniline as its sole carbon and nitrogen source. Genomic sequence analysis revealed that this bacterium possesses putative gene clusters for biodegradation of various monocyclic aromatic hydrocarbons (MAHs), including benzene, toluene, and xylene (BTX), as well as aniline. We verified the proposed MAH biodegradation pathways by dioxygenase activity assays, RT-PCR, and LC/MS-based quantitative proteomic analyses. This proteogenomic approach revealed four independent degradation pathways, all converging into the citric acid cycle. Aniline and p-hydroxybenzoate degradation pathways converged into the β-ketoadipate pathway. Benzoate and toluene were degraded through the benzoyl-CoA degradation pathway. The xylene isomers, i.e., o-, m-, and p-xylene, were degraded via the extradiol cleavage pathways. Salicylate was degraded through the gentisate degradation pathway. Our results show that Burkholderia sp. K24 possesses versatile biodegradation pathways, which may be employed for efficient bioremediation of aniline and BTX.
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Sang-Yeop
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Gun-Hwa
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yun
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Chi-Won
authors.initials: CW
authors.lastname: Choi
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.|Department of Food Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Yoon-Sun
authors.initials: YS
authors.lastname: Yi
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Jonghyun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Chung
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Edmond Changkyun
authors.initials: EC
authors.lastname: Park
authors.affiliation: Drug & Disease Target Team, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.|Bio-Analysis Science, University of Science & Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000272:Adipates / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001554:Benzene / Q000378:metabolism; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000235:genetics / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D063990:Gene Ontology; D005841:Gentisates / Q000378:metabolism; D058977:Molecular Sequence Annotation; D020156:Salicylic Acid / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D000272:Adipates; D000814:Aniline Compounds; D001426:Bacterial Proteins; D005841:Gentisates; D012989:Soil Pollutants; D014992:Xylenes; C027316:3-oxoadipic acid; D014050:Toluene; C037817:benzoyl-coenzyme A; D049308:Dioxygenases; D001554:Benzene; D020156:Salicylic Acid; C023650:aniline