title: Imaging of enzyme activity using bio-LSI system enables simultaneous immunosensing of different analytes in multiple specimens.
abstract: Electrochemical imaging is an excellent technique to characterize an activity of biomaterials, such as enzymes and cells. Large scale integration-based amperometric sensor (Bio-LSI) has been developed for the simultaneous and continuous detection of the concentration distribution of redox species generated by reactions of biomolecules. In this study, the Bio-LSI system was demonstrated to be applicable for simultaneous detection of different anaytes in multiple specimens. The multiple specimens containing human immunoglobulin G (hIgG) and mouse IgG (mIgG) were introduced into each channel of the upper substrate across the antibody lines for hIgG and mIgG on the lower substrate. Hydrogen peroxide generated by the enzyme reaction of glucose oxidase captured at intersections was simultaneously detected by 400 microelectrodes of Bio-LSI chip. The oxidation current increased with increasing the concentrations of hIgG, which can be detected in the range of 0.01-1.0 µg mL(-1) . Simultaneous detection of hIgG and mIgG in multiple specimens was achieved by using line pattern of both antibodies. Therefore, the presence of different target molecules in the multiple samples would be quantitatively and simultaneously visualized as a current image by the Bio-LSI system.
authors.affiliation: Graduate School of Material Science, University of Hyogo, Hyogo, Kamigori, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Hokuto
authors.affiliation: Graduate School of Material Science, University of Hyogo, Hyogo, Kamigori, Japan. yasu@sci.u-hyogo.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yasukawa
authors.affiliation: Japan Aviation Electronics Industry, Limited, Tokyo, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Kunikata
authors.affiliation: Japan Aviation Electronics Industry, Limited, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suda
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Miyagi, Sendai, Japan.
authors.forename: Kumi Y
authors.initials: KY
authors.lastname: Inoue
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Miyagi, Sendai, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Miyagi, Sendai, Japan.|The World Premier International Research Center-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Miyagi, Sendai, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
authors.affiliation: Graduate School of Material Science, University of Hyogo, Hyogo, Kamigori, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D007074:Immunoglobulin G / Q000032:analysis*; D051379:Mice; D008839:Microelectrodes; D013045:Species Specificity
chemical_list: D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
keywords: Amperometric sensor; Electrochemical biosensing; Enzyme immunoassay; Multiple specimens; Simultaneous imaging