title: [Changes of Tim-3 and PD-1 on peripheral blood monocyte subsets in patients with chronic hepatitis C].
abstract: OBJECTIVE
  To investigate the distribution of peripheral blood monocyte subsets of chronic hepatitis C (CHC) patients and observe the expression of negative regulators T cell immunoglobulin and mucin domain-3 (Tim-3) and programmed cell death-1 (PD-1) on the monocyte subsets.
  
  
  METHODS
  Flow cytometry was employed to determine the distribution of three monocyte subsets as well as Tim-3 and PD-1 expression on the three monocyte subsets. Their correlations with the clinical parameters were analyzed by Spearman test.
  
  
  RESULTS
  Compared with healthy controls, an increased distribution of CD14(+)CD16(+) monocytes, especially CD14(++)CD16(+) monocyte subset, was observed in CHC patients. Tim-3 expression was significantly elevated on CD14(++)CD16(-) and CD14(+)CD16(++) subsets in CHC patients. Obviously increased PD-1 expression was found mainly on CD14(++)CD16(-) and CD14(++)CD16(+) subsets. There were no significant correlations between monocyte subsets, PD-1, Tim-3 and the clinical parameters.
  
  
  CONCLUSIONS
  The levels Tim-3 and PD-1 are different in three monocyte subsets.
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038, China.
authors.forename: Peixin
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yi
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038, China.
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038, China. *Corresponding author, E-mail: zyfmmu@hotmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D058851:GPI-Linked Proteins / Q000097:blood; D000072597:Hepatitis A Virus Cellular Receptor 2; D019698:Hepatitis C, Chronic / Q000097:blood*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry*; D061026:Programmed Cell Death 1 Receptor / Q000097:blood*; D017452:Receptors, IgG / Q000097:blood
chemical_list: C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D018950:Lipopolysaccharide Receptors; D008565:Membrane Proteins; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D017452:Receptors, IgG