title: Aqueous leaf extract of Ocimum gratissimum improves hematological parameters in alloxan-induced diabetic rats via its antioxidant properties.
abstract: OBJECTIVE
  This study was designed to investigate the effects of Ocimum gratissimum (OG) on hematological parameters and oxidative stress in diabetic rats.
  
  
  METHODS
  Twenty-five male rats (150-200 g) were randomly grouped into five as control, normal + OG, diabetic untreated, diabetic + OG, and diabetic + glibenclamide groups. Diabetes was induced by 100 mg/kg of alloxan monohydrate in the diabetic untreated and diabetic + OG groups followed by treatment with distilled water and 400 mg/kg OG, respectively, whereas control, normal + OG, and diabetic + glibenclamide groups were treated with distilled water, 400 mg/kg OG, and 5 mg/kg glibenclamide, respectively. Body weight and fasting blood glucose level were monitored weekly. After 28 days of treatments, under anesthesia induced by 50 mg/kg sodium thiopental i.p., blood samples were obtained for hematological analysis, malondialdehyde (MDA) level determination, and superoxide dismutase (SOD) activity. Data were compared using analysis of variance and Student's t-test.
  
  
  RESULTS
  There was a significant decrease in the fasting blood glucose of the diabetic + OG animals compared to the diabetic untreated and the initial reduction in weight observed in this group was reversed at the end of the experiments. Packed cell volume, red blood cell count, and hemoglobin concentration were significantly increased (P < 0.05) in the diabetic + OG when compared with the untreated group. The MDA concentration was significantly lowered (P < 0.01) in the diabetic + OG group when compared with diabetic untreated while SOD activity was significantly reduced in the diabetic untreated group.
  
  
  CONCLUSIONS
  It was concluded that OG reverses anemia secondary to alloxan-induced diabetes mellitus in rats probably via its antioxidant activity.
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Shehu-Tijani Toyin
authors.initials: ST
authors.lastname: Shittu
authors.affiliation: Department of Physiology, Igbinedion University, Okada, Edo State, Nigeria.
authors.forename: Wahab A
authors.initials: WA
authors.lastname: Oyeyemi
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Taye J
authors.initials: TJ
authors.lastname: Lasisi
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Seyid Alli-Siise
authors.initials: SA
authors.lastname: Shittu
authors.affiliation: Department of Animal Science, University of Ibadan, Ibadan, Nigeria.
authors.forename: Temitope T
authors.initials: TT
authors.lastname: Lawal
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: Samuel T
authors.initials: ST
authors.lastname: Olujobi
keywords: Antioxidant; Ocimum gratissimum; hematological parameters; lipid peroxidation