title: Formation and characterization of β-cyclodextrin (β-CD) - polyethyleneglycol (PEG) - polyethyleneimine (PEI) coated Fe3O4 nanoparticles for loading and releasing 5-Fluorouracil drug.
abstract: In this work, β-cyclodextrin (β-CD) - polyethyleneglycol (PEG) - polyethyleneimine (PEI) coated iron oxide nanoparticles (Fe3O4-β-CD-PEG-PEI) were developed as drug carriers for drug delivery applications. The 5- Fluorouracil (5-FU) was chosen as model drug molecule. The developed nanoparticles (Fe3O4-β-CD-PEG-PEI) were characterized by various techniques such as Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM). The average particles size range of 5-FU loaded Fe3O4-β-CD, Fe3O4-β-CD-PEG and Fe3O4-β-CD-PEG-PEI nanoparticles were from 151 to 300nm and zeta potential value of nanoparticles were from -43mV to -20mV as measured using Malvern Zetasizer. Finally, encapsulation efficiency (EE), loading capacity (LC) and in-vitro drug release performance of 5-FU drug loaded Fe3O4-β-CD, Fe3O4-β-CD-PEG and Fe3O4-β-CD-PEG-PEI nanoparticles was evaluated by UV-vis spectroscopy. In-vitro cytotoxicity tests investigated by MTT assay indicate that 5-FU loaded Fe3O4-β-CD-PEG-PEI nanoparticles were toxic to cancer cells and non-toxic to normal cells. The in-vitro release behavior of 5-FU from drug (5-FU) loaded Fe3O4-β-CD-PEG-PEI composite at different pH values and temperature was studied. It was found that 5-FU was released faster in pH 6.8 than in the acidic mediums (pH 1.2), and the released quantity was higher. Therefore, the newly prepared Fe3O4-β-CD-PEG-PEI carrier exhibits a promising potential capability for anticancer drug delivery in tumor therapy.
authors.affiliation: Advanced Materials Research Laboratory, Department of Chemistry, Periyar University, Salem-11, Tamil Nadu, India. Electronic address: gprabhagovinn@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Prabha
authors.affiliation: Advanced Materials Research Laboratory, Department of Chemistry, Periyar University, Salem-11, Tamil Nadu, India. Electronic address: alaguraj2@rediffmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Raj
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D065546:Drug Liberation; D005290:Ferric Compounds / Q000737:chemistry*; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D060328:Magnetic Phenomena; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D047392:beta-Cyclodextrins; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); C000499:ferric oxide; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D005472:Fluorouracil
keywords: 5-Fluorouracil (5-FU); Drug delivery; Encapsulation; Iron oxide nanoparticles; Polyethylene glycol (PEG); Polyethyleneimine (PEI); β-cyclodextrin (β-CD)