title: Rapid and enzyme-free nucleic acid detection based on exponential hairpin assembly in complex biological fluids.
abstract: Herein, we have developed a rapid and enzyme-free nucleic acid amplification detection method that combined the exponential self-assembly of four DNA hairpins and the FRET pair Cy3 and Cy5. This strategy was very ingenious and rapid, and could detect nucleic acids at concentrations as low as 10 pM in 15 min in biological fluids.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. sc169@163.com and Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. mcp169@163.com.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Ma
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. sc169@163.com and Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. mcp169@163.com.
authors.forename: Menghua
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Northwest Agriculture and Forestry University, Xianyang 712100, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. sc169@163.com and Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. mcp169@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. sc169@163.com and Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. mcp169@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Shi
mesh_terms: D015374:Biosensing Techniques; D004247:DNA; D021141:Nucleic Acid Amplification Techniques; D009696:Nucleic Acids / Q000032:analysis*
chemical_list: D009696:Nucleic Acids; D004247:DNA