title: Human Dental Pulp Stem Cells and Gingival Fibroblasts Seeded into Silk Fibroin Scaffolds Have the Same Ability in Attracting Vessels.
abstract: Neovascularization is one of the most important processes during tissue repair and regeneration. Current healing approaches based on the use of biomaterials combined with stem cells in critical-size bone defects fail due to the insufficient implant vascularization and integration into the host tissues. Therefore, here we studied the attraction, ingrowth, and distribution of blood vessels from the chicken embryo chorioallantoic membrane into implanted silk fibroin scaffolds seeded with either human dental pulp stem cells or human gingival fibroblasts. Perfusion capacity was evaluated by non-invasive in vivo Magnetic Resonance Imaging while the number and density of blood vessels were measured by histomorphometry. Our results demonstrate that human dental pulp stem cells and gingival fibroblasts possess equal abilities in attracting vessels within silk fibroin scaffolds. Additionally, the prolonged in vitro pre-incubation period of these two cell populations favors the homogeneous distribution of vessels within silk fibroin scaffolds, which further improves implant survival and guarantees successful healing and regeneration.
authors.affiliation: Orofacial Development and Regeneration, Center of Dental Medicine, Institute of Oral Biology, University of Zurich Zurich, Switzerland.
authors.forename: Anna
authors.initials: A
authors.lastname: Woloszyk
authors.affiliation: Plastic Surgery and Hand Surgery, University Hospital Zurich Zurich, Switzerland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Buschmann
authors.affiliation: Institute for Biomedical Engineering, ETH and University of ZurichZurich, Switzerland; Visceral and Transplant Surgery, University Hospital ZurichZurich, Switzerland.
authors.forename: Conny
authors.initials: C
authors.lastname: Waschkies
authors.affiliation: Clinic of Cranio-Maxillofacial and Oral Surgery, University of Zurich, University Hospital Zurich Zurich, Switzerland.
authors.forename: Bernd
authors.initials: B
authors.lastname: Stadlinger
authors.affiliation: Orofacial Development and Regeneration, Center of Dental Medicine, Institute of Oral Biology, University of Zurich Zurich, Switzerland.
authors.forename: Thimios A
authors.initials: TA
authors.lastname: Mitsiadis
keywords: chorioallantoic membrane (CAM); human dental pulp stem cells (hDPSCs); human gingival fibroblasts (hGFs); mesenchymal stem cells; regenerative medicine; silk fibroin scaffolds; vascularization