title: Isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens.
abstract: Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bacteria from swine milk have been published. In this work, we isolated several lactic acid bacteria from swine milk and evaluated them for them potential as probiotics. Among the isolated strains, Lactobacillus curvatus TUCO-5E showed antagonistic effects against swine-associated gastrointestinal pathogens. TUCO-5E was able to reduce the growth of enterotoxigenic and enterohemorrhagic Escherichia coli strains as well as pathogenic salmonella. In vitro exclusion and displacement assays in intestinal epithelial cells showed a remarkable antagonistic effect for L. curvatus TUCO-5E against Salmonella sp. strain TUCO-I7 and Salmonella enterica ATCC 13096. Moreover, by using a mouse model of Salmonella infection, we were able to demonstrate that preventative administration of L. curvatus TUCO-5E for 5 consecutive days was capable of decreasing the number of Salmonella enterica serovar Typhimurium in the liver and spleen of treated mice, compared with the controls, and prevented dissemination of the pathogen to the blood stream. Therefore, we have demonstrated here that swine milk is an interesting source of beneficial bacteria. In addition, the results of this work suggest that L. curvatus TUCO-5E is a good candidate to study in vivo the protective effect of probiotics against intestinal infection and damage induced by Salmonella infection in the porcine host.
authors.affiliation: a Laboratory of Food Microbiology, Faculty of Veterinary Sciences, University of Concepción, Chillán, Chile.
authors.forename: Sandra Rayén
authors.initials: SR
authors.lastname: Quilodrán-Vega
authors.affiliation: b Immunobiotics Research Group, Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman, Argentina.
authors.forename: Julio
authors.initials: J
authors.lastname: Villena
authors.affiliation: a Laboratory of Food Microbiology, Faculty of Veterinary Sciences, University of Concepción, Chillán, Chile.
authors.forename: José
authors.initials: J
authors.lastname: Valdebenito
authors.affiliation: c Laboratory of Bacterial Pathogenicity, Microbiology Department, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: María José
authors.initials: MJ
authors.lastname: Salas
authors.affiliation: c Laboratory of Bacterial Pathogenicity, Microbiology Department, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: Cristian
authors.initials: C
authors.lastname: Parra
authors.affiliation: a Laboratory of Food Microbiology, Faculty of Veterinary Sciences, University of Concepción, Chillán, Chile.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Ruiz
authors.affiliation: d Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.|e Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.affiliation: f Immunobiotics Research Group, Laboratory of Bacterial Pathogenicity, Microbiology Department, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.
authors.forename: Apolinaria
authors.initials: A
authors.lastname: García
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D024881:Drug Resistance, Bacterial; D041981:Gastrointestinal Tract / Q000382:microbiology; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology; D008099:Liver / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000382:microbiology*; D019936:Probiotics / Q000008:administration & dosage*; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000254:growth & development*; D013154:Spleen / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*
chemical_list: D006460:Hemolysin Proteins; D019344:Lactic Acid
keywords: Lactobacillus curvatus TUCO-5; Lactobacillus curvatus TUCO-5E; Salmonella; infection intestinale; intestinal infection; lait porcin; pigs; porcs; probiotics; probiotiques; swine milk