title: Nanomedicine: Interaction of biomimetic apatite colloidal nanoparticles with human blood components.
abstract: This contribution investigates the interaction of two types of biomimetic-apatite colloidal nanoparticles (negatively-charged 47nm, and positively-charged 190nm NPs) with blood components, namely red blood cells (RBC) and plasma proteins, with the view to inspect their hemocompatibility. The NPs, preliminarily characterized by XRD, FTIR and DLS, showed low hemolysis ratio (typically lower than 5%) illustrating the high compatibility of such NPs with respect to RBC, even at high concentration (up to 10mg/ml). The presence of glucose as water-soluble matrix for freeze-dried and re-dispersed colloids led to slightly increased hemolysis as compared to glucose-free formulations. NPs/plasma protein interaction was then followed, via non-specific protein fluorescence quenching assays, by contact with whole human blood plasma. The amount of plasma proteins in interaction with the NPs was evaluated experimentally, and the data were fitted with the Hill plot and Stern-Volmer models. In all cases, binding constants of the order of 10(1)-10(2) were found. These values, significantly lower than those reported for other types of nanoparticles or molecular interactions, illustrate the fairly inert character of these colloidal NPs with respect to plasma proteins, which is desirable for circulating injectable suspensions. Results were discussed in relation with particle surface charge and mean particle hydrodynamic diameter (HD). On the basis of these hemocompatibility data, this study significantly complements previous results relative to the development and nontoxicity of biomimetic-apatite-based colloids stabilized by non-drug biocompatible organic molecules, intended for use in nanomedicine.
authors.affiliation: CIRIMAT, Universite de Toulouse, CNRS, INPT, UPS, Ensiacet, Toulouse, France.
authors.forename: Maëla
authors.initials: M
authors.lastname: Choimet
authors.affiliation: Nano Bio Materials Laboratory, Dept. Chemistry and Medical Chemistry, Yonsei University, Wonju, South Korea.
authors.forename: Kim
authors.initials: K
authors.lastname: Hyoung-Mi
authors.affiliation: Nano Bio Materials Laboratory, Dept. Chemistry and Medical Chemistry, Yonsei University, Wonju, South Korea. Electronic address: jaemin.oh@yonsei.ac.kr.
authors.forename: Oh
authors.initials: O
authors.lastname: Jae-Min
authors.affiliation: CIRIMAT, Universite de Toulouse, CNRS, INPT, UPS, Ensiacet, Toulouse, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Tourrette
authors.affiliation: CIRIMAT, Universite de Toulouse, CNRS, INPT, UPS, Ensiacet, Toulouse, France. Electronic address: christophe.drouet@ensiacet.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Drouet
mesh_terms: D001031:Apatites / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry; D003102:Colloids / Q000737:chemistry*; D005612:Freeze Drying; D005947:Glucose / Q000737:chemistry; D006801:Humans; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D010949:Plasma / Q000737:chemistry
chemical_list: D001031:Apatites; D001798:Blood Proteins; D003102:Colloids; D005947:Glucose
keywords: Apatite nanoparticles; Blood cells; Hemocompatibility; Hemolysis; Protein fluorescence quenching