title: [Distribution of Formic Acid after Methanol Intoxication in Rats].
abstract: OBJECTIVE
  To investigate concentration and distribution in blood and tissues of formic acid after methanol intoxication in rats.
  
  
  METHODS
  The Sprague-Dawley rats were divided into groups for control group and 3-day and 7-day intoxication treatment groups. The experimental groups were administered methanol by gavage with the initial dose of 8 mL/kg and followed with 4 mL/kg supplemental dose 24 h later. After 3 days and 7 days later, rats were killed by decapitation. Then samples of cardiac blood, liver, kidney, brain, heart and stomach of each group were collected. Formic acid concentrations were detected by high performance liquid chromatography.
  
  
  RESULTS
  Formic acid concentrations in tissues were higher than in blood. Compared with 3-day intoxication group, there was an increase formic acid of concentration in brain and stomach in 7-day intoxication group, while a decrease in liver and kidney (P < 0.05).
  
  
  CONCLUSIONS
  High performance liquid chromatography could be used to accurately detect formic acid. As the metabolite of methanol, formic acid accumulates in rat blood and tissues after intoxication and the concentrations in organs and tissues are obviously higher than in blood.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Liu
authors.forename: Shu
authors.initials: S
authors.lastname: Zhou
authors.forename: Jie-min
authors.initials: JM
authors.lastname: Chen
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Xia
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005561:Formates / Q000097:blood*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D000432:Methanol / Q000097:blood* / Q000506:poisoning*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D005561:Formates; C030544:formic acid; D000432:Methanol