title: Dispersion of hydrophobic magnetic nanoparticles using ultarsonic-assisted in combination with coacervative microextraction for the simultaneous preconcentration and determination of tricyclic antidepressant drugs in biological fluids.
abstract: A two-step sample preparation technique based on dispersive micro solid-phase extraction combined with coacervative microextraction is presented for preconcentration and determination of tricyclic antidepressant drugs in biological samples. An important feature of the method is the application of hydrophobic magnetic nanoparticles, which in combination with coacervative microextraction method enables development of rapid and efficient extraction procedure in order to achievement of a high extraction efficiency. Simultaneous optimization by experimental design lead to improvement of method with low cost which supply useful information about interaction among variables. Under the optimized conditions, a linear range of 5-1000ngmL(-1) with detection limits from 0.51 to 1.4ngmL(-1) were obtained for target analytes. The method was successfully used for the determination of analytes in biological fluids (plasma and urine) with relative recoveries in the range of 89-105% (RSDs<3.5%).
authors.affiliation: Department of Pathology, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Jannesar
authors.affiliation: Department of Chemistry, Faculty of Sciences, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Zare
authors.affiliation: Department of Chemistry, Faculty of Sciences, Yasouj University, Yasouj 75918-74831, Iran. Electronic address: m_ghaedi@yahoo.com.
authors.forename: Mehrorang
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: Department of Chemistry, Faculty of Sciences, Ilam University, Ilam 69315-516, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Daneshfar
mesh_terms: D000929:Antidepressive Agents, Tricyclic / Q000032:analysis*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D052616:Solid Phase Extraction
chemical_list: D000929:Antidepressive Agents, Tricyclic; D058185:Magnetite Nanoparticles
keywords: Coacervative microextraction; Dispersive micro solid phase extraction; Tricyclic antidepressant drugs; Ultrasonic-assisted