title: Ameliorating Effects of Sphingomyelin-Based Liposomes on Sarcopenia in Senescence-Accelerated Mice.
abstract: The effects of orally administered sphingomyelin-based liposomes (SM-lipo) on muscle function were investigated in senescence-accelerated mice prone 1 (SAMP1) for the purpose of protection against or treatment of sarcopenia. SM-lipo were prepared by thin lipid-film hydration followed by extrusion. Their spherical shape was observed by transmission electron microscopy. The obtained liposomes were stable in gastric liquid and intestinal fluid models as well as in water. In in vitro tests liposomalization of sphingomyelin significantly increased its transport into human intestinal epithelial Caco-2 cells. In addition, SM-lipo upregulated the proliferation of murine C2C12 myoblasts compared with free sphingomyelin or phosphatidylcholine-based liposomes (PC-lipo). Finally, SM-lipo orally administered to SAMP1 for 10 weeks significantly increased quadriceps femoris weight and extended swimming time until fatigue compared with PC-lipo. In conclusion, these findings indicate that SM-lipo are well absorbed into the body and improve muscle weakness caused by senescence.
authors.affiliation: Department of Medical Biochemistry, Graduate School of Pharmaceutical Sciences, University of Shizuoka.
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Ishida
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kiyokawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Asai
authors.forename: Naoto
authors.initials: N
authors.lastname: Oku
mesh_terms: D000375:Aging; D000818:Animals; D018938:Caco-2 Cells; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008081:Liposomes; D008297:Male; D051379:Mice; D018908:Muscle Weakness / Q000188:drug therapy; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects; D055948:Sarcopenia / Q000188:drug therapy*; D013109:Sphingomyelins / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use
chemical_list: D008081:Liposomes; D013109:Sphingomyelins