title: Neuraminidase Resistant Sialosides for the Detection of Influenza Viruses.
abstract: We report the synthesis of influenza virus neuraminidase (NA) resistant sialosides that include different glycoside linkages (C-, S-, and triazole). These unnatural sialosides were printed onto glass slides to generate a small focused microarray. We evaluated the binding affinity of multiple lectins and compared the stability of these sialosides with O-linked sialosides toward influenza virus neuraminidase and intact virus. We demonstrated the ability of these molecules to capture eight different strains of influenza virus at ambient temperature without the addition of NA inhibitors. The glycans capture extremely low, clinically relevant concentrations of viruses and each strain gives rise to a specific "fingerprint" binding pattern, which could potentially be used in rapid diagnostic tests.
authors.affiliation: Petit Science Center, Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30302 United States.
authors.forename: Yun
authors.initials: Y
authors.lastname: He
authors.affiliation: Petit Science Center, Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30302 United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Petit Science Center, Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30302 United States.
authors.forename: Suri S
authors.initials: SS
authors.lastname: Iyer
mesh_terms: D015374:Biosensing Techniques; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism; D053118:Influenza A Virus, H1N1 Subtype / Q000201:enzymology / Q000302:isolation & purification*; D037102:Lectins / Q000378:metabolism; D057230:Limit of Detection; D009439:Neuraminidase / Q000378:metabolism*; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D006027:Glycosides; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D037102:Lectins; D011134:Polysaccharides; D009439:Neuraminidase