title: Coupling end resection with the checkpoint response at DNA double-strand breaks.
abstract: DNA double-strand breaks (DSBs) are a nasty form of damage that needs to be repaired to ensure genome stability. The DSB ends can undergo a strand-biased nucleolytic processing (resection) to generate 3'-ended single-stranded DNA (ssDNA) that channels DSB repair into homologous recombination. Generation of ssDNA also triggers the activation of the DNA damage checkpoint, which couples cell cycle progression with DSB repair. The checkpoint response is intimately linked to DSB resection, as some checkpoint proteins regulate the resection process. The present review will highlight recent works on the mechanism and regulation of DSB resection and its interplays with checkpoint activation/inactivation in budding yeast.
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Villa
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.
authors.forename: Corinne
authors.initials: C
authors.lastname: Cassani
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Gobbini
authors.affiliation: Institute of Molecular Biology gGmbH (IMB), 55128, Mainz, Germany.
authors.forename: Diego
authors.initials: D
authors.lastname: Bonetti
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy. mariapia.longhese@unimib.it.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Longhese
mesh_terms: D059447:Cell Cycle Checkpoints; D053903:DNA Breaks, Double-Stranded; D004720:Endonucleases / Q000378:metabolism; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D004720:Endonucleases
keywords: Double-strand break; MRX; Mec1; Nucleases; Resection; Tel1