title: Micromonospora yasonensis sp. nov., isolated from a Black Sea sediment.
abstract: A Micromonospora strain, designated DS3186(T), isolated from sediment collected from the Black Sea off the Yason Peninsula, Ordu, Turkey, was examinated using a polyphasic approach. The strain was found to have chemotaxonomic, morphological and phylogenetic properties consistent with its clasification in the genus Micromonospora. A comparative 16S rRNA gene sequence analysis showed that the strain was closely related to the type strains of Micromonospora olivasterospora (99.0 %), Micromonospora equina (98.8 %), Micromonospora rhizosphaerae (98.8 %) and Micromonospora viridifaciens (98.8 %); low levels of DNA-DNA relatednes were found between the isolate and the M. olivasterospora and M. rhizosphaerae strains. Corresponding phylogenetic analysis based on partial gyrB gene sequences showed that strain DS3186(T) formed a subclade with the type strains of Micromonospora eburnea, M. equina, Micromonospora narathiwatensis and M. viridifaciens. Strain DS3186(T) was distinguished from its close phylogenetic neighbours using a combination of chemotaxonomic, morphological and physiological properties. Consequently, it is proposed that strain DS3186(T) represents a novel Micromonospora species for which the name Micromonospora yasonensis sp. nov. is proposed. The type strain is DS3186(T) (=DSM 45980(T) = KCTC 29433(T)).
authors.affiliation: Department of Bioengineering, Faculty of Engineering and Architecture, Sinop University, 57000, Nasuhbasoglu-Sinop, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Veyisoglu
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Lorena
authors.initials: L
authors.lastname: Carro
authors.affiliation: Biology Department, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey.
authors.forename: Kiymet
authors.initials: K
authors.lastname: Guven
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139, Kurupelit-Samsun, Turkey. nsahin@omu.edu.tr.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
mesh_terms: D015373:Bacterial Typing Techniques; D059038:Black Sea; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014421:Turkey; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D027081:DNA Gyrase
keywords: 16S rRNA gene; Actinobacteria; Micromonospora yasonensis sp. nov.; Polyphasic taxonomy; gyrB gene