title: Derivation and Utilization of Functional CD8(+) Dendritic Cell Lines.
abstract: It is notoriously difficult to obtain large quantities of non-activated dendritic cells ex vivo. For this reason, we produced and characterized a mouse model expressing the large T oncogene under the CD11c promoter (Mushi mice), in which CD8α(+) dendritic cells transform after 4 months. We derived a variety of stable cell lines from these primary lines. These cell lines reproducibly share with freshly isolated dendritic cells most surface markers, mRNA and protein expression, and all tested biological functions. Cell lines can be derived from various strains and knockout mice and can be easily transduced with lentiviruses. In this article, we describe the derivation, culture, and lentiviral transduction of these dendritic cell lines.
authors.affiliation: Department of Biochemistry CIIL, University of Lausanne, Chemin des Boveresses 155, CH-1066, Epalinges, Switzerland.
authors.forename: Matteo
authors.initials: M
authors.lastname: Pigni
authors.affiliation: Department of Biochemistry CIIL, University of Lausanne, Chemin des Boveresses 155, CH-1066, Epalinges, Switzerland.
authors.forename: Devika
authors.initials: D
authors.lastname: Ashok
authors.affiliation: Department of Biochemistry CIIL, University of Lausanne, Chemin des Boveresses 155, CH-1066, Epalinges, Switzerland. hans.acha-orbea@unil.ch.
authors.forename: Hans
authors.initials: H
authors.lastname: Acha-Orbea
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D039521:CD11c Antigen / Q000235:genetics*; D016827:CD8 Antigens / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000952:Antigens, Polyomavirus Transforming; D039521:CD11c Antigen; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain
keywords: Cell line; Dendritic cell; Ex vivo derivation; Tissue culture