title: MicroRNA-140-5p impairs zebrafish embryonic bone development via targeting BMP-2.
abstract: MicroRNA-140-5p (miRNA-140-5p) is important for embryonic bone development. In this study, we found that miRNA-140-5p and its binding site in the 3'UTR of bone morphogenetic protein 2 (BMP-2) are highly conserved among vertebrates, and miRNA-140-5p negatively regulates both zebrafish and human BMP-2 genes. Microinjection of miRNA-140-5p or BMP-2b morpholino into zebrafish embryos led to a similar phenotype, including shortened tails, curved trunks, and defects in cranial cartilage. Moreover, miRNA-140-5p injection induced zebrafish embryo malformation that could be significantly rescued by microinjection of BMP-2 mRNA. In conclusion, our results indicated that miRNA-140-5p regulates zebrafish embryonic bone development via targeting BMP-2.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Shiquan
authors.initials: S
authors.lastname: Gan
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Zhaoqin
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|College of Medicine, Hunan Normal University, Changsha, Hunan, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Renxiang
authors.initials: R
authors.lastname: Su
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Guie
authors.initials: G
authors.lastname: Xie
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, Hunan, China.|The Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), Changsha, Hunan, China.
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Xiang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001846:Bone Development; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D017124:Conserved Sequence; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D014714:Vertebrates / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D055396:Bone Morphogenetic Protein 2; C559197:MIRN140 microRNA, zebrafish; D035683:MicroRNAs; D029961:Zebrafish Proteins; C405936:bmp2b protein, zebrafish
keywords: BMP-2; bone and cartilage development; embryos; microRNA-140-5p; zebrafish