title: First molecular detection and phylogenetic analysis of Anaplasma phagocytophilum in shelter dogs in Seoul, Korea.
abstract: In this study, the status of Anaplasma phagocytophilum infection was assessed in shelter dogs in Seoul, Korea, with PCR and phylogenetic analyses. Nested PCR on 1058 collected blood samples revealed only one A. phagocytophilum positive sample (female, age <1year, mixed breed, collected from the north of the Han River). The genetic variability of A. phagocytophilum was evaluated by genotyping, using the 16S rRNA, groEL, and msp2 gene sequences of the positive sample. BLASTn analysis revealed that the 16S rRNA, groEL, and msp2 genes had 99.6%, 99.9%, and 100% identity with the following sequences deposited in GenBank: a cat 16S rRNA sequence from Korea (KR021166), a rat groEL sequence from Korea (KT220194), and a water deer msp2 sequence from Korea (HM752099), respectively. Phylogenetic analyses classified the groEL gene into two distinct groups (serine and alanine), whereas the msp2 gene showed a general classification into two groups (USA and Europe) that were further subgrouped according to region. To the best of our knowledge, this study is the first to describe the molecular diagnosis of A. phagocytophilum in dogs reared in Korea. In addition, the high genetic identity of the 16S rRNA and groEL sequences between humans and dogs from the same region suggests a possible epidemiological relation. Given the conditions of climate change, tick ecology, and recent incidence of human granulocytic anaplasmosis in Korea, the findings of this study underscore the need to establish appropriate control programs for tick-borne diseases in Korea.
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Sukyee
authors.initials: S
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Seung-Hun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Dorene
authors.initials: D
authors.lastname: VanBik
authors.affiliation: Seoul Metropolitan Government Research Institute of Public Health & Environment, Gwacheon, Gyunggi, Republic of Korea.
authors.forename: Neung-Hee
authors.initials: NH
authors.lastname: Kim
authors.affiliation: Animal Health Center of Zoo Land, Daejeon O-World Theme Park, Daejeon, Republic of Korea.
authors.forename: Kyoo-Tae
authors.initials: KT
authors.lastname: Kim
authors.affiliation: Department of Parasitology and Tropical Medicine, College of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Goo
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea; Cardiovascular Research Institute, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Man Hee
authors.initials: MH
authors.lastname: Rhee
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Oh-Deog
authors.initials: OD
authors.lastname: Kwon
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea; Cardiovascular Research Institute, Kyungpook National University, Daegu, Republic of Korea. Electronic address: dmkwak@knu.ac.kr.
authors.forename: Dongmi
authors.initials: D
authors.lastname: Kwak
mesh_terms: D041081:Anaplasma phagocytophilum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics; D057231:Climate Change; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D066106:Seoul / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000382:microbiology*; D015047:Zoonoses
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C450839:p44 protein, Anaplasma phagocytophila
keywords: Anaplasma phagocytophilum; PCR; Phylogeny; Shelter dogs; Zoonosis