title: LYR3, a high-affinity LCO-binding protein of Medicago truncatula, interacts with LYK3, a key symbiotic receptor.
abstract: LYR3, LYK3, and NFP are lysin motif-containing receptor-like kinases (LysM-RLKs) from Medicago truncatula, involved in perception of symbiotic lipo-chitooligosaccharide (LCO) signals. Here, we show that LYR3, a high-affinity LCO-binding protein, physically interacts with LYK3, a key player regulating symbiotic interactions. In vitro, LYR3 is phosphorylated by the active kinase domain of LYK3. Fluorescence lifetime imaging/Förster resonance energy transfer (FLIM/FRET) experiments in tobacco protoplasts show that the interaction between LYR3 and LYK3 at the plasma membrane is disrupted or inhibited by addition of LCOs. Moreover, LYR3 attenuates the cell death response, provoked by coexpression of NFP and LYK3 in tobacco leaves.
authors.affiliation: LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Fliegmann
authors.affiliation: Plateforme Imagerie-Microscopie, Fédération de Recherche FR3450 - Agrobiosciences, Interactions et Biodiversité, CNRS, Université de Toulouse, UPS, Castanet-Tolosan, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Jauneau
authors.affiliation: Fédération de Recherche FR3450 - Agrobiosciences, Interactions et Biodiversité, CNRS, Université de Toulouse, UPS, Castanet-Tolosan, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Pichereaux
authors.affiliation: LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Rosenberg
authors.affiliation: LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Gasciolli
authors.affiliation: LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
authors.forename: Antonius C J
authors.initials: AC
authors.lastname: Timmers
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Burlet-Schiltz
authors.affiliation: LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Cullimore
authors.affiliation: LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Bono
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D008070:Lipopolysaccharides / Q000378:metabolism*; D046913:Medicago truncatula / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*; D013559:Symbiosis; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D010940:Plant Proteins; C023023:lipid-linked oligosaccharides; D011494:Protein Kinases
keywords: FLIM/FRET; LysM-RLK; Medicago truncatula; lipo-chitooligosaccharides; membrane receptor complex; phosphoproteomics