title: Microfluidic-based generation of functional microfibers for biomimetic complex tissue construction.
abstract: Microfluidic-based fiber system displays great potential in reconstructing naturally complex tissues. In these systems, fabrication of the basic fiber is a significant factor in ensuring a functional construction. The fiber should possess the strong mechanical rigidity for assembly, predefined microenvironment for cell spatial distribution and high biocompatibility for cell functional expression. Herein we presented a composite material by the combination of methacrylated gelatin (GelMA) and alginate for fiber engineering with capillary microfluidic device. Being regulated by GelMA incorporation, the composite hydrogels exhibited higher mechanical moduli, better stretching performance, and lower swelling compared to pure alginate one. On the basis of the composite material and capillary microfluidic device, we constructed the double-layer hollow microfibers to simulate complex tissues. The microfibers could be precisely controlled in size and multi-layered structure by varying flow rates and outlet diameter, and it showed satisfied application in woven-structure assembly. As an example to mimic a functional tissue, a biomimetic osteon-like structure was fabricated by encapsulating human umbilical vascular endothelial cells (HUVECs) in middle layer to imitate vascular vessel and human osteoblast-like cells (MG63) in the outer layer to act role of bone. During the incubation period, both MG63 and HUVECs exhibited not only a robust growth, but also up-regulated gene expression. These results demonstrated this microfluidic-based composite microfibers system is a promising alternative in complex tissue regeneration.
  
  
  
  Cell-laden microfibers based on microfluidic device is attracting interest for reconstructing naturally complex tissues. One shortage is the lack of suitable materials which satisfy microfluidic fabrication and cell biofunctional survival. This study reports the first combination of alginate-GelMA composite and capillary-based microfluidic technology. The composite materials possess high mechanical properties for fabrication and assembly, and tunable environment for cell spatial encapsulation. Significantly, the engineered double-layer hollow microfiber with osteon-like structure showed enhanced cellular bioactivity and realized initially functional establishment. This microfluidic-based composite microfiber not only explores a competitive candidate in complex tissues reconstruction, but also expands the biological application of microfluidic technology. This developing interdisciplinary area should be widely interested to the readers of biofabrication, biomaterials and tissue engineering.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Yicong
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: He
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: You
authors.initials: Y
authors.lastname: Yang
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhong
authors.affiliation: School of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xie
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China. Electronic address: hsfan@scu.edu.cn.
authors.forename: Hongsong
authors.initials: H
authors.lastname: Fan
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610065, People's Republic of China.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002460:Cell Line; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
keywords: Alginate; Methacrylated gelatin; Microfibers; Microfluidic; Osteon