title: Technical note: use of internal transcribed spacer for ruminal yeast identification in dairy cows.
abstract: Molecular techniques are important tools for microbiological studies in different habitats, and the internal transcribed spacer (ITS) has been proved to be useful for analyzing fungal diversity. The aim of this study was to use the ITS region to generate ruminal yeast profile and to identify ruminal yeast. DNA from ruminal digesta was extracted to amplify the ribosomal ITS region. The profile from the PCR products was visualized and the excised bands from the profile were identified as the genera Millerozyma, Pichia, Rhizomucor and Hyphopichia. Overall, the ITS resulted to be a simple, fast and sensitive approach that allowed profiling and identification of ruminal yeast that have not been previously described (Millerozyma and Hyphopichia) in the rumen microbial community.
authors.affiliation: 1Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal,Pontificia Universidad Católica de Chile,Santiago,Casilla 306,C.P. 6904411,Chile.
authors.forename: E
authors.initials: E
authors.lastname: Vargas-Bello-Pérez
authors.affiliation: 1Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal,Pontificia Universidad Católica de Chile,Santiago,Casilla 306,C.P. 6904411,Chile.
authors.forename: N
authors.initials: N
authors.lastname: Cancino-Padilla
authors.affiliation: 2Laboratorio de Biotecnología,Instituto de Nutrición y Tecnología de los Alimentos (INTA),Universidad de Chile,Santiago,C.P. 7810000,Chile.
authors.forename: J
authors.initials: J
authors.lastname: Romero
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology / Q000502:physiology*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006863:Hydrogen-Ion Concentration; D012417:Rumen / Q000382:microbiology*; D012441:Saccharomyces cerevisiae; D012680:Sensitivity and Specificity; D015002:Yeast, Dried / Q000235:genetics*
chemical_list: D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers
keywords: dairy cow; internal transcribed spacer; rumen; yeast identification