title: Hibiscus Sabdariffa L. Flowers and Olea Europea L. Leaves Extract-Based Formulation for Hypertension Care: In Vitro Efficacy and Toxicological Profile.
abstract: Olea europaea L. leaves extract (Oe) and Hybiscus sabdariffa L. flowers extract (Hs) have calcium antagonistic properties. Aim of this work was to study the cardiovascular effects of Pres Phytum(®), a nutraceutical formulation containing a mixture of the two extracts and the excipients, and investigate its possible off-target effects, using in vitro biological assays on guinea pig isolated organs. Cardiovascular effects were assessed using guinea pig atria and aorta. The effects of Pres Phytum on spontaneous gastrointestinal, urinary, and respiratory tracts smooth muscle contractility were evaluated. Pres Phytum exerted a vasorelaxant effect (IC50 = 2.38 mg/mL) and a negative chronotropic effect (IC50 = 1.04 mg/mL) at concentrations lower than those producing smooth muscle spontaneous contractility alterations in the other organs. Compared to Pres Phytum, the mixture did not exert negative inotropic activity, while it maintained a negative chronotropic efficacy (IC50 = 1.04 mg/mL). These experimental data suggest a possible nutraceutical use of this food supplement for the management of preclinical hypertension.
authors.affiliation: 1 Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna , Bologna, Italy .
authors.forename: Matteo
authors.initials: M
authors.lastname: Micucci
authors.affiliation: 2 Department of Specialistic, Experimental, Diagnostic and Specialty Medicine (DIMES), Section of Nephrology, Alma Mater Studiorum-University of Bologna , S. Orsola Hospital, Bologna, Italy .
authors.forename: Andrea
authors.initials: A
authors.lastname: Angeletti
authors.affiliation: 3 National Institute of Biostructure and Biosystems (INBB), Interuniversity Consortium , Rome, Italy .
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Cont
authors.affiliation: 4 Department of Specialistic, Experimental, Diagnostic and Specialty Medicine (DIMES), Centro di Coordinamento dell'attività fisico-medica, Alma Mater Studiorum-University of Bologna , S. Orsola Hospital, Bologna, Italy .
authors.forename: Ivan
authors.initials: I
authors.lastname: Corazza
authors.affiliation: 1 Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna , Bologna, Italy .
authors.forename: Rita
authors.initials: R
authors.lastname: Aldini
authors.affiliation: 1 Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna , Bologna, Italy .
authors.forename: Elisa
authors.initials: E
authors.lastname: Donadio
authors.affiliation: 1 Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna , Bologna, Italy .
authors.forename: Alberto
authors.initials: A
authors.lastname: Chiarini
authors.affiliation: 1 Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna , Bologna, Italy .
authors.forename: Roberta
authors.initials: R
authors.lastname: Budriesi
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000503:physiopathology; D004353:Drug Evaluation, Preclinical; D035264:Flowers / Q000737:chemistry; D006168:Guinea Pigs; D006325:Heart Atria / Q000187:drug effects / Q000503:physiopathology; D031584:Hibiscus / Q000009:adverse effects / Q000737:chemistry*; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000503:physiopathology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D031658:Olea / Q000009:adverse effects / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000009:adverse effects / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D010936:Plant Extracts
keywords: dietary herbal supplement; herbal drugs; herbal extract; hypertension; medicinal plant