title: MicroRNA-370-3p inhibits human glioma cell proliferation and induces cell cycle arrest by directly targeting β-catenin.
abstract: The aim of this study was to explore the expression and biological role of miR-370-3p in human gliomas.
  
  
  
  Clinical specimens from the brains of 20 glioma patients and 10 healthy controls were obtained to quantify the expression level of miR-370-3p using quantitative real-time PCR. Oligonucleotide mimics of miR-370-3p were transfected into U251 and U87-MG cells for a gain of function assay. The CCK-8 assay, colony formation assay, EdU assay and flow cytometry were used to evaluate the roles of miR-370-3p in cell proliferation and the cell cycle regulation. Western blot and luciferase activity assays were used to investigate the reciprocal relationship between miR-370-3p and its predicted target, β-catenin.
  
  
  
  miR-370-3p expression was frequently found to be decreased in glioma tissues, and its expression level was negatively correlated with the malignant degree of the glioma. Overexpression of miR-370-3p showed a significant inhibitory effect on cell proliferation and accompanied cell cycle G0/G1 arrest in U251 and U87-MG cells. Furthermore, miR-370-3p inhibited the expression of the canonical Wnt pathway downstream targets cyclin D1 and c-myc via direct binding interaction with the 3'-untranslated region of β-catenin mRNA. Reintroduction of β-catenin could partially reverse the anti-proliferation effect of miR-370-3p. Finally, in 20 glioma tissues the expression of miR-370-3p was negatively correlated with both protein and mRNA levels of β-catenin.
  
  
  
  miR-370-3p suppresses glioma cell growth by directly targeting β-catenin, suggesting that the miR-370-3p/β-catenin axis may be a target for glioma therapy.
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
authors.forename: Zesheng
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
authors.forename: Tingfeng
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
authors.forename: Shenqi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
authors.forename: Qianxue
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China. Electronic address: tiandaofeng@hotmail.com.
authors.forename: Daofeng
authors.initials: D
authors.lastname: Tian
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; C526503:MIRN370 microRNA, human; D035683:MicroRNAs; D051176:beta Catenin
keywords: Cell cycle; Glioma; Proliferation; miR-370-3p; β-catenin