title: Development and pharmacological evaluation of in vitro nanocarriers composed of lamellar silicates containing copaiba oil-resin for treatment of endometriosis.
abstract: In this work, newly developed nanocomposites based upon lamellar silicates are evaluated to determine their potential in controlling endometriosis. The preparation of the new nanocarriers is detailed, properties characterized and in vitro pharmacological evaluation performed. The nanocomposites in this study were obtained from the reaction of copaiba oil-resin (COPA) with the polymer polyvinylpyrrolidone (PVP K-30). COPA was selected due to its antiinflammatory and anticancer activities along with the organophilic derivatives of sodium montmorillonite, Viscogel B8, S7 and S4. The results indicated that it was feasible to obtain a good yield of a COPA nanocomposite using a simple process. Intercalation was confirmed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). In vitro release experiments demonstrated that COPA was released from the nanocomposite in a delayed fashion. Whereas, in vitro pharmacological studies showed a reduction in viability and proliferation of endometriotic cell cultures upon COPA nanocomposite treatment, suggesting that the system developed here can be a promising alternative therapy for the oral treatment of endometriosis.
authors.affiliation: Department of Drugs and Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Vinícius Raphael
authors.initials: VR
authors.lastname: de Almeida Borges
authors.affiliation: Programa de Pesquisa em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Julianna Henriques
authors.initials: JH
authors.lastname: da Silva
authors.affiliation: Department of Drugs and Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Samantha Soares
authors.initials: SS
authors.lastname: Barbosa
authors.affiliation: Programa de Pesquisa em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Luiz Eurico
authors.initials: LE
authors.lastname: Nasciutti
authors.affiliation: Department of Drugs and Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Lúcio Mendes
authors.initials: LM
authors.lastname: Cabral
authors.affiliation: Department of Drugs and Pharmaceutics, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: valeria@pharma.ufrj.br.
authors.forename: Valeria Pereira
authors.initials: VP
authors.lastname: de Sousa
mesh_terms: D001453:Balsams / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D004715:Endometriosis / Q000188:drug therapy* / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D001453:Balsams; D004337:Drug Carriers; D017640:Silicates; C114971:copaiba balsam
keywords: Copaiba oil-resin; Endometriosis; Lamellar silicates; Montmorillonite; Nanotechnology; Organophilic clays