title: Intact glycopeptide characterization using mass spectrometry.
abstract: Glycosylation is one of the most prominent and extensively studied protein post-translational modifications. However, traditional proteomic studies at the peptide level (bottom-up) rarely characterize intact glycopeptides (glycosylated peptides without removing glycans), so no glycoprotein heterogeneity information is retained. Intact glycopeptide characterization, on the other hand, provides opportunities to simultaneously elucidate the glycan structure and the glycosylation site needed to reveal the actual biological function of protein glycosylation. Recently, significant improvements have been made in the characterization of intact glycopeptides, ranging from enrichment and separation, mass spectroscopy (MS) detection, to bioinformatics analysis. In this review, we recapitulated currently available intact glycopeptide characterization methods with respect to their advantages and limitations as well as their potential applications.
authors.affiliation: a Pharma Research and Development , R&D Platform Technology & Science, GSK , King of Prussia , PA , USA.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.affiliation: b ChemEco Division , Evans Analytical Group , Hercules , CA , USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qu
authors.affiliation: c Process Research & Development , AbbVie , North Chicago , IL , USA.
authors.forename: Zhaorui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: d Department of Chemistry and Biochemistry , University of Oklahoma , Norman , OK , USA.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: d Department of Chemistry and Biochemistry , University of Oklahoma , Norman , OK , USA.
authors.forename: Iya
authors.initials: I
authors.lastname: Prytkova
authors.affiliation: d Department of Chemistry and Biochemistry , University of Oklahoma , Norman , OK , USA.
authors.forename: Si
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D054794:Glycomics / Q000379:methods*; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods*
chemical_list: D006020:Glycopeptides
keywords: Glycosylation; LC-MS/MS; bioinformatics; glycopeptide; post-translational modification; proteomics