title: Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates.
abstract: BACKGROUND
  The agar dilution method is currently considered as the reference method for Mycobacterium marinum drug susceptibility testing (DST). As it is time-consuming, alternative methods, such as the E-test, were evaluated for M. marinum DST, but without success. The SLOMYCO Sensititre(®) panel, recently commercialized by TREK Diagnostic Systems (Cleveland, OH), can be used for DST in slow-growing mycobacteria and for antimicrobial agents recommended by the Clinical and Laboratory Standards Institute (CLSI) for M. marinum DST. The main goal of this work was to evaluate the SLOMYCO Sensititre(®) panel method for DST in M. marinum isolates from human patients and fish relative to the reference agar dilution method.
  
  
  RESULTS
  The reproducibility of the minimum inhibitory concentration (MIC) determination (±1 log2 dilution) was very good for both the agar dilution method and SLOMYCO Sensititre(®) panel (>90 % agreement). The percentage essential agreement between methods varied, depending on the drug: between 97 and 75 % for ciprofloxacin, moxifloxacin, linezolid, isoniazid, clarithromycin, amikacin, rifabutin and rifampin, 74 % for trimethoprim, 72 % for doxycycline, 70 % for sulfamethoxazole, 59 % for streptomycin, 33 % for ethambutol and only 2.2 % for ethionamide. When the agar dilution and SLOMYCO Sensititre(®) panel results were converted into interpretive criteria, the category agreement was 100 % for amikacin, ciprofloxacin, clarithromycin, moxifloxacin, rifabutin, sulfamethoxazole and trimethoprim, 98 % for ethambutol and 96 % for rifampin and no agreement for doxycycline.
  
  
  CONCLUSIONS
  The SLOMYCO Sensititre(®) panel method could provide a potential alternative to the reference agar dilution method, when DST in M. marinum is required, except for doxycycline.
authors.affiliation: Département de Bactériologie-Virologie, CHRU Montpellier, Montpellier, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Chazel
authors.affiliation: Département de Bactériologie-Virologie, CHRU Montpellier, Montpellier, France.|UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, Laboratoire de Bactériologie, U.F.R. des Sciences Pharmaceutiques et Biologiques, Montpellier, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Marchandin
authors.affiliation: Laboratoire Départemental Vétérinaire de l'Hérault, Montpellier, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Keck
authors.affiliation: Département de Bactériologie-Virologie, CHRU Montpellier, Montpellier, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Terru
authors.affiliation: Département de Bactériologie-Virologie, CHRU Montpellier, Montpellier, France.|Department of Bacteriology-Virology, INSERM U1058 "Pathogenesis and Control of Chronic Infections", Université Montpellier-EFS, CHU Arnaud de Villeneuve 371 avenue du Doyen Gaston Giraud, 34295, Montpellier, France.|Université Montpellier, Montpellier, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Carrière
authors.affiliation: Laboratoire Biomnis, Lyon, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Ponsoda
authors.affiliation: Laboratoire Biomnis, Lyon, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Jacomo
authors.affiliation: Laboratoire Biomnis, Lyon, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Panteix
authors.affiliation: Département de Bactériologie-Virologie, CHRU Montpellier, Montpellier, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bouzinbi
authors.affiliation: MIVEGEC (Laboratoire Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle), UMR CNRS 5290/IRD 224, Université de Montpellier, Montpellier, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Bañuls
authors.affiliation: MIVEGEC (Laboratoire Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle), UMR CNRS 5290/IRD 224, Université de Montpellier, Montpellier, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Choisy
authors.affiliation: Université Montpellier, Montpellier, France.|Department of Biopathology, CHRU Montpellier, Montpellier, France.|Department of Clinical Oncoproteomic, Montpellier Cancer Institute, Montpellier, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Solassol
authors.affiliation: Centre National de Référence des Mycobactéries et de la Résistance aux Antituberculeux, Paris, France.|Laboratoire de Bactériologie-Hygiène Paris, AP-HP, Hôpital Pitié-Salpêtrière, 75013, Paris, France.|Centre for Immunology and Microbial Infections, team 13, Sorbonne Universités, UPMC Univ Paris 06, U1135, 75013, Paris, France.|INSERM, U1135, Paris, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Aubry
authors.affiliation: Département de Bactériologie-Virologie, CHRU Montpellier, Montpellier, France. s-godreuil@chu-montpellier.fr.|Department of Bacteriology-Virology, INSERM U1058 "Pathogenesis and Control of Chronic Infections", Université Montpellier-EFS, CHU Arnaud de Villeneuve 371 avenue du Doyen Gaston Giraud, 34295, Montpellier, France. s-godreuil@chu-montpellier.fr.|Université Montpellier, Montpellier, France. s-godreuil@chu-montpellier.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Godreuil
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D019910:Mycobacterium marinum / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents
keywords: Agar dilution method; Antimicrobial susceptibility testing; Human and fish isolates; Mycobacterium marinum; SLOMYCO Sensititre® panel