title: Agrobacterium-mediated inoculation of chrysanthemum (Chrysanthemum morifolium) plants with chrysanthemum stunt viroid.
abstract: Agroinfiltration was tested as a method of inoculation of chrysanthemum plants with chrysanthemum stunt viroid (CSVd). Binary vectors harboring dimeric CSVd sequences in sense and antisense orientations were constructed, and Agrobacterium transfected with these binary vectors was infiltrated into chrysanthemum leaves. Northern blotting and reverse transcription polymerase chain reaction analysis showed that local infection was established within 7 days and systemic infection within 20 days. CSVd polarities showed no difference in infectivity. This study showed that agroinfiltration of chrysanthemum plants is an easy, rapid, and cost-effective method for CSVd inoculation.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: nabeshima.tomoyuki.68w@st.kyoto-u.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nabeshima
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: dmotoaki@kais.kyoto-u.ac.jp.
authors.forename: Motoaki
authors.initials: M
authors.lastname: Doi
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: mune@kais.kyoto-u.ac.jp.
authors.forename: Munetaka
authors.initials: M
authors.lastname: Hosokawa
mesh_terms: D060054:Agrobacterium / Q000821:virology*; D031202:Chrysanthemum / Q000382:microbiology* / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D014772:Viroids
keywords: Agrobacterium; Chrysanthemum molifolium; Chrysanthemum stunt viroid; Infiltration; Viroid infection