title: Assessing histidine tags for recruiting deoxyribozymes to catalyze peptide and protein modification reactions.
abstract: We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). However, the recruiting benefits of the histidine tag are not observed for larger protein substrates, likely because the tested deoxyribozymes either cannot access the target peptide segments or cannot function when these segments are presented in a structured protein context.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA. sks@illinois.edu.
authors.forename: Chih-Chi
authors.initials: CC
authors.lastname: Chu
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA. sks@illinois.edu.
authors.forename: Scott K
authors.initials: SK
authors.lastname: Silverman
mesh_terms: D055162:Biocatalysis; D021881:DNA, Catalytic / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D010455:Peptides; D011506:Proteins; D006639:Histidine; D012313:RNA