title: Development of Specific Markers for Identification of Biovars 1 and 2 Strains of Pseudomonas syringae pv. actinidiae.
abstract: Pseudomonas syringae pv. actinidiae, the causal agent of canker in kiwifruit, can be divided into three biovars (biovars 1, 2, and 3). Strains belonging to biovar 1 produce phaseolotoxin and were isolated in Japan and Italy before 2008. Strains of biovar 2 produce coronatine instead of phaseolotoxin and have been isolated only in Korea. Strains belonging to biovar 3 produce neither phaseolotoxin nor coronatine and are responsible for the global outbreak of bacterial canker of kiwifruit in recent years. The biovar 3-specific primer set was developed in a previous work. In this study, two sets of PCR primers specific to strains of biovars 1 and 2, respectively, were developed based on random amplified polymorphic DNA analyses. Primers PsaJ-F and PsaJ-R produced a 481-bp region with genomic DNA of biovar 1 strains, whereas primers PsaK-F and PsaK-R amplified a 413-bp region present only in the genome of biovar 2 strains.
authors.affiliation: Department of Biology, Sunchon National University, Sunchon 57922, Korea.
authors.forename: Young Sun
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Plant Medicine, Sunchon National University, Sunchon 57922, Korea.
authors.forename: Gyoung Hee
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Department of Plant Medicine, Sunchon National University, Sunchon 57922, Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Koh
authors.affiliation: Sichuan Provincial Academy of Natural Resources, Chengdu 610015, China.
authors.forename: Qiguo
authors.initials: Q
authors.lastname: Zhuang
authors.affiliation: Department of Biology, Sunchon National University, Sunchon 57922, Korea.
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Jung
keywords: Pseudomonas syringae pv. actinidiae; bacterial canker; biovar; kiwifruit; molecular marker