title: GidA, a tRNA Modification Enzyme, Contributes to the Growth, and Virulence of Streptococcus suis Serotype 2.
abstract: Glucose-inhibited division protein (GidA), is a tRNA modification enzyme functioning together with MnmE in the addition of a carboxymethylaminomethyl group to position 5 of the anticodon wobble uridine of tRNA. Here, we report a GidA homolog from a Chinese isolate SC-19 of the zoonotic Streptococcus suis serotype 2 (SS2). gidA disruption led to a defective growth, increased capsule thickness, and reduced hemolytic activity. Moreover, the gidA deletion mutant (ΔgidA) displayed reduced mortality and bacterial loads in mice, reduced ability of adhesion to and invasion in epithelial cells, and increased sensitivity to phagocytosis. The iTRAQ analysis identified 372 differentially expressed (182 up- and 190 down-regulated) proteins in ΔgidA and SC-19. Numerous DNA replication, cell division, and virulence associated proteins were downregulated, whereas many capsule synthesis enzymes were upregulated by gidA disruption. This is consistent with the phenotypes of the mutant. Thus, GidA is a translational regulator that plays an important role in the growth, cell division, capsule biosynthesis, and virulence of SS2. Our findings provide new insight into the regulatory function of GidA in bacterial pathogens.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China; Veterinary Medicine Laboratory, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural ScienceWuhan, China; Wuhan Chopper Biology Co., Ltd.Wuhan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
authors.forename: Meifang
authors.initials: M
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
authors.forename: Wanquan
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Veterinary Medicine Laboratory, Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Science Wuhan, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University Wuhan, China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China; Cooperative Innovation Center of Sustainable Pig ProductionWuhan, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China; Cooperative Innovation Center of Sustainable Pig ProductionWuhan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics*; D058491:Bacterial Load / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D006801:Humans; D051379:Mice; D010587:Phagocytosis / Q000235:genetics / Q000276:immunology; D012343:RNA, Transfer / Q000235:genetics; D017011:Streptococcus suis / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; C108512:glucose-inhibited division protein A, bacteria; D012343:RNA, Transfer
keywords: Streptococcus suis (S. suis); capsule synthesis; glucose-inhibited division protein (GidA); growth; iTRAQ; tRNA modification; virulence