title: Differential expression of microRNAs and potential targets under drought stress in barley.
abstract: Drought is a crucial environmental constraint limiting crop production in many parts of the world. microRNA (miRNA) based gene regulation has been shown to act in several pathways, including crop response to drought stress. Sequence based profiling and computational analysis have revealed hundreds of miRNAs and their potential targets in different plant species under various stress conditions, but few have been biologically verified. In this study, 11 candidate miRNAs were tested for their expression profiles in barley. Differences in accumulation of only four miRNAs (Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c) were observed between drought-treated and well-watered barley in four genotypes. miRNA targets were predicted using degradome analysis of two, different genotypes, and genotype-specific target cleavage was observed. Inverse correlation of mature miRNA accumulation with miRNA target transcripts was also genotype dependent under drought treatment. Drought-responsive miRNAs accumulated predominantly in mesophyll tissues. Our results demonstrate genotype-specific miRNA regulation under drought stress and evidence for their role in mediating expression of target genes for abiotic stress response in barley.
authors.affiliation: Australian Centre for Plant Functional Genomics, PMB1, Glen Osmond, SA, 5064, Australia.|School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.
authors.forename: Jannatul
authors.initials: J
authors.lastname: Ferdous
authors.affiliation: Australian Centre for Plant Functional Genomics, PMB1, Glen Osmond, SA, 5064, Australia.|Phenomics and Bioinformatics Research Centre, School of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes, SA, 5095, Australia.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanchez-Ferrero
authors.affiliation: School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Langridge
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.
authors.forename: Linda
authors.initials: L
authors.lastname: Milne
authors.affiliation: School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.|ARC Centre of Excellence in Plant Cell Walls, PMB1, Glen Osmond, SA, 5064, Australia.
authors.forename: Jamil
authors.initials: J
authors.lastname: Chowdhury
authors.affiliation: Phenomics and Bioinformatics Research Centre, School of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes, SA, 5095, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Brien
authors.affiliation: Australian Centre for Plant Functional Genomics, PMB1, Glen Osmond, SA, 5064, Australia.|School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.
authors.forename: Penny J
authors.initials: PJ
authors.lastname: Tricker
mesh_terms: D001483:Base Sequence; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D001467:Hordeum / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics; D020871:RNA Stability / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Hordeum vulgare; canonical cleavage; degradome; genotype; in situ-RT-PCR