title: Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran.
abstract: BACKGROUND
  Candida parapsilosis is an emergent agent of invasive fungal infections. This yeast is one of the five most widespread yeasts concerned in invasive candidiasis. C. parapsilosis stands out as the second most common yeast species isolated from patients with bloodstream infections especially in neonates with catheter. Recently several reports suggested that its reduced susceptibility to azoles and polyene might become a cause for clinical concern, although C. parapsilosis is not believed to be intensely prone to the development of antifungal resistance.
  
  
  METHODS
  In the present report, One hundred and twenty clinical isolates of C. parapsilosis complex were identified and differentiated by using PCR-RFLP analysis. The isolates were then analyzed to determine their susceptibility profile to fluconazole (FLU), itraconazole (ITC) and amphotericin B. The minimum inhibitory concentration (MIC) results were analyzed according to the standard CLSI guide.
  
  
  RESULTS
  All of isolates were identified as C. parapsilosis. No C. metapsilosis and C. orthopsilosis strains were found. Evaluation of the antifungal susceptibility profile showed that only three (2.5%) C. parapsilosis were resistant to fluconazole, three (2.5%) C. parapsilosis were resistant to itraconazole and two (1.7%) C. parapsilosis were amphotericin B resistant.
  
  
  CONCLUSIONS
  Profiles in clinical isolates of C. parapsilosis can provide important information for the control of antifungal resistance as well as distribution and susceptibility profiles in populations.
authors.affiliation: Dept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ensieh
authors.initials: E
authors.lastname: Lotfali
authors.affiliation: Dept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Parivash
authors.initials: P
authors.lastname: Kordbacheh
authors.affiliation: Dept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mirhendi
authors.affiliation: Dept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Zaini
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Ghajari
authors.affiliation: Dept. of Medical, Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Rasoul
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: Dept. of Biology, Faculty of Science, Islamic Azad University, Varamin-Pishva, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Noorbakhsh
authors.affiliation: Invasive Fungi Research Center, Dept. of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Moazeni
authors.affiliation: Dept. of Microbiology & Parasitology & Immunology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Fallahi
authors.affiliation: Dept. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sassan
authors.initials: S
authors.lastname: Rezaie
keywords: Antifungal susceptibility; Candida parapsilosis; Iran; Resistant