title: Biochanin A improves hepatic steatosis and insulin resistance by regulating the hepatic lipid and glucose metabolic pathways in diet-induced obese mice.
abstract: Natural compounds that regulate peroxisome proliferator-activated receptor alpha (PPARα) have been reported to have beneficial effects in obesity-mediated metabolic disorders. In this study, we demonstrated that biochanin A (BA), an agonist of PPAR-α, improved hepatic steatosis and insulin resistance by regulating hepatic lipid and glucose metabolism.
  
  
  
  C57BL/6 mice were fed a normal chow diet, a high-fat diet (HFD), and an HFD supplemented with 0.05% BA for 12 weeks. Histological and biochemical examinations indicated that BA prevented obesity-induced hepatic steatosis and insulin resistance in HFD-fed mice. BA stimulated the transcriptional activation of PPAR-α in vitro and increased the expression of PPAR-α and its regulatory proteins in the liver. CE-TOF/MS analyses indicated that BA administration promoted the recovery of metabolites involved in phosphatidylcholine synthesis, lipogenesis, and beta-oxidation in the livers of obese mice. BA also suppressed the levels of gluconeogenesis-related metabolites and the expression of the associated enzymes, glucose 6-phosphatase and pyruvate kinase.
  
  
  
  Taken together, these results showed that BA ameliorated metabolic disorders such as hepatic steatosis and insulin resistance by modulating lipid and glucose metabolism in diet-induced obesity. Thus, BA may be a potential therapeutic agent for the prevention of obesity-mediated hepatic steatosis and insulin resistance.
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea.
authors.forename: Hee-Sook
authors.initials: HS
authors.lastname: Park
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea.
authors.forename: Haeng Jeon
authors.initials: HJ
authors.lastname: Hur
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea.
authors.forename: Soon-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea.|Department of Food Biotechnology, University of Science & Technology, Seongnam, Republic of Korea.
authors.forename: Moon Ju
authors.initials: MJ
authors.lastname: Hong
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea.|Department of Food Biotechnology, University of Science & Technology, Seongnam, Republic of Korea.
authors.forename: Mi Jeong
authors.initials: MJ
authors.lastname: Sung
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea.|Department of Food Biotechnology, University of Science & Technology, Seongnam, Republic of Korea.
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.affiliation: Division of Nutrition and Metabolism, Korea Food Research Institute, Seongnam, Republic of Korea. truka@kfri.re.kr.|Department of Food Biotechnology, University of Science & Technology, Seongnam, Republic of Korea. truka@kfri.re.kr.
authors.forename: Myung-Sunny
authors.initials: MS
authors.lastname: Kim
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects; D019833:Genistein / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007333:Insulin Resistance; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism
chemical_list: D047493:PPAR alpha; D010713:Phosphatidylcholines; D019833:Genistein; D005947:Glucose; C004541:biochanin A
keywords: Biochanin A; Glucose metabolism; Hepatic steatosis; Lipid metabolism; Metabolomics