title: Visible-light driven biofuel cell based on hierarchically branched titanium dioxide nanorods photoanode for tumor marker detection.
abstract: In this work, a novel sensing platform based on visible light driven biofuel cell (BFC) has been facilely designed for sensitive detection of prostate-specific antigen (PSA) with the photo-response bioanode, realizing the dual route energy conversion of light energy and chemical energy to electricity. The hierarchical branched TiO2 nanorods (B-TiO2 NRs) decorated with CdS quantum dots (QDs) act as the substrate to confine glucose dehydrogenase (GDH) for the visible light driven glucose oxidation at the bioanode. Three dimensional flowers like hierarchical carbon/gold nanoparticles/bilirubin oxidase (3D FCM/AuNPs/BOD) bioconjugate served as biocatalyst for O2 reduction at the biocathode. With an increase in the concentration of PSA, the amount of BOD labels on biocathode increases, thus leading to the higher current output of the as-proposed visible light driven BFC. Based on this, this sensing platform provide great performance in sensitivity and specificity, increasing linear detection range from 0.3pgmL(-1) to 7μgmL(-1) with a detection limit of 0.1pgmL(-1). Most importantly, our new sensing strategy provided a simple and inexpensive sensing platform for tumor markers detection, suggesting its wide potential applications for clinical diagnostics.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, PR China.
authors.forename: Chaomin
authors.initials: C
authors.lastname: Gao
authors.affiliation: Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, PR China.
authors.forename: Yanhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, PR China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Cancer Research Center, Shandong Tumor Hospital, Jinan 250012, PR China.
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
mesh_terms: D001674:Bioelectric Energy Sources; D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D006801:Humans; D008027:Light; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D017430:Prostate-Specific Antigen / Q000097:blood*; D018036:Selenium Compounds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D019187:Cadmium Compounds; D004800:Enzymes, Immobilized; D018036:Selenium Compounds; C009495:titanium dioxide; C058667:cadmium selenide; D014025:Titanium; D042843:Glucose 1-Dehydrogenase; D017430:Prostate-Specific Antigen
keywords: Biofuel cell; Branched TiO(2) nanorods; CdS quantum dots; Flower-like Hierarchical carbon