title: 4-Chloro-6-pyrimidinylferrocene modified silica gel: A novel multiple-function stationary phase for mixed-mode chromatography.
abstract: A novel multi-function and mixed-mode stationary phase based on 4-chloro-6-pyrimidinylferrocene modified silica (NFcS) was synthesized and characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. Linear solvation energy relationship method was successfully employed to evaluate the new phase with a set of 27 solutes including aromatic and aliphatic compounds. Multiple mechanisms including hydrophobic, π-π, hydrogen-bonding, charge-transfer, acid-base equilibrium and anion-exchange interactions are involved. Based on these interactions, successful separation could be achieved among polycyclic aromatic hydrocarbons, mono-substituted benzenes, aromatic amines, phenols, quinolines, pyridines and nucleosides in reversed-phase (RP) or normal-phase (NP) chromatography. Inorganic anions were also shown to be individually separated in anion-exchange chromatography by using the same column. Moreover, the results here also demonstrated that NFcS based stationary phase could effectively reduce the adverse effect of residual silanol in the separation process. Such stationary phase with characteristics of multi-interaction mechanism and mixed-mode separation is potential for the analysis of complex samples. The NFcS column was successfully employed for the analysis of plant growth regulators in Fruit.
authors.affiliation: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Qiao
authors.affiliation: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Yanhao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: yuajuan@zzu.edu.cn.
authors.forename: Ajuan
authors.initials: A
authors.lastname: Yu
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450008, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Hu
authors.affiliation: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: zsszz@126.com.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Yangjie
authors.initials: Y
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005296:Ferrous Compounds; D057927:Hydrophobic and Hydrophilic Interactions; D000075163:Metallocenes; D010636:Phenols; D058428:Silica Gel; D012822:Silicon Dioxide
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D010636:Phenols; D058428:Silica Gel; D012822:Silicon Dioxide; C004998:ferrocene
keywords: Ferrocene stationary phase; Linear solvation energy relationship; Mixed-mode; Multi-function; Retention mechanism