title: A reduced graphene oxide-based fluorescence resonance energy transfer sensor for highly sensitive detection of matrix metalloproteinase 2.
abstract: A novel fluorescence nanoprobe (reduced nano-graphene oxide [nrGO]/fluorescein isothiocyanate-labeled peptide [Pep-FITC]) for ultrasensitive detection of matrix metalloproteinase 2 (MMP2) has been developed by engineering the Pep-FITC comprising the specific MMP2 substrate domain (PLGVR) onto the surface of nrGO particles through non-covalent linkage. The nrGO was obtained by water bathing nano-graphene oxide under 90°C for 4 hours. After mixing the nrGO and Pep-FITC for 30 seconds, the fluorescence from Pep-FITC was almost completely quenched due to the fluorescence resonance energy transfer between fluorescein isothiocyanate (FITC) and nrGO. Upon cleavage of the amide bond between Leu and Gly in the Pep-FITC by protease-MMP2, the FITC bound to nrGO was separated from nrGO surface, disrupting the fluorescence resonance energy transfer process and resulting in fluorescence recovery of FITC. Under optimal conditions, the fluorescence recovery of nrGO/Pep-FITC was found to be directly proportional to the concentration of MMP2 within 0.02-0.1 nM. The detection limit of the nrGO/Pep-FITC was determined to be 3 pM, which is approximately tenfold lower than that of the unreduced carboxylated nano-graphene oxide/Pep-FITC probe.
authors.affiliation: MOE Key Laboratory of Laser Life Science & College of Biophotonics, South China Normal University, Guangzhou, People's Republic of China.
authors.forename: Gaina
authors.initials: G
authors.lastname: Xi
authors.affiliation: Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou, People's Republic of China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Laser Life Science & College of Biophotonics, South China Normal University, Guangzhou, People's Republic of China.
authors.forename: Tongsheng
authors.initials: T
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D020778:Matrix Metalloproteinase 2 / Q000097:blood* / Q000302:isolation & purification*; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010087:Oxides; D006108:Graphite; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: fluorescence; graphene oxide; high sensitive detection; reduction