title: Malassezia arunalokei sp. nov., a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India.
abstract: The majority of species within the genus Malassezia are lipophilic yeasts that colonize the skin of warm-blooded animals. Two species, Malassezia globosa and Malassezia restricta, are implicated in the causation of seborrheic dermatitis/dandruff (SD/D). During our survey of SD/D cases, we isolated several species of Malassezia and noticed vast variations within a few lipid-dependent species. Variations observed in the phenotypic characteristics (colony morphology, absence of catalase activity, growth at 37°C, and precipitation surrounding wells containing Tween 20 or Cremophor EL) suggested the possible presence of a novel species. Sequence divergence observed in the internal transcribed spacer (ITS) region, the D1/D2 domain, and the intergenic spacer 1 (IGS1) region of rDNA and the TEF1 gene, PCR-restriction fragment length polymorphism (RFLP) analysis of the ITS2 region, and fluorescent amplified fragment length polymorphism analysis support the existence of a novel species. Based on phenotypic and molecular characterization of these strains, we propose a new species, namely, M. arunalokei sp. nov., and we designate NCCPF 127130 (= MTCC 12054 = CBS 13387) as the type strain.
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Prasanna
authors.initials: P
authors.lastname: Honnavar
authors.affiliation: Microbial Type Culture Collection and Gene Bank, Council of Scientific and Industrial Research-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Gandham S
authors.initials: GS
authors.lastname: Prasad
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Anup
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Department of Dermatology, Venerology, and Leprosy, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Dogra
authors.affiliation: Department of Dermatology, Venerology, and Leprosy, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Handa
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India mrshivprakash@yahoo.com.
authors.forename: Shivaprakash M
authors.identifier: http://orcid.org/0000-0002-9097-9253
authors.initials: SM
authors.lastname: Rudramurthy
mesh_terms: D000293:Adolescent; D000818:Animals; D016022:Case-Control Studies; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D012628:Dermatitis, Seborrheic / Q000175:diagnosis* / Q000382:microbiology; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D006801:Humans; D007194:India; D008290:Malassezia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008297:Male; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011446:Prospective Studies; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S