title: Effect of residual sericin on the structural characteristics and properties of regenerated silk films.
abstract: Regenerated silk film has been increasingly attracting the research community's attention for biomedical applications due to its good biocompatibility and excellent cyto-compatibility. However, some limitations regarding its mechanical properties, such as brittleness, have restricted the use of silk films for industrial biomedical applications. In this study, regenerated silk films with different residual sericin content were prepared applying controlled degumming conditions to evaluate the effect of sericin content on the structure and properties of the films generated. When the residual sericin content increased to 0.6%, crystallinity index and breaking strength of silk films were increased. Above this value, these parameters then decreased. A 1.5 fold increase of silk film elongation properties was obtained when incorporating 16% sericin. Regardless of sericin content, all regenerated silk films showed excellent cyto-compatibility, comparable to the one obtained with tissue culture plates.
authors.affiliation: Department of Bio-fibers and Materials Science, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Dae Woong
authors.initials: DW
authors.lastname: Song
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
authors.affiliation: Department of Bio-fibers and Materials Science, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: icum@knu.ac.kr.
authors.forename: In Chul
authors.initials: IC
authors.lastname: Um
mesh_terms: D000818:Animals; D012831:Bombyx; D002254:Carbonates / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004548:Elasticity; D006244:Hardness; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D019301:Oleic Acid / Q000737:chemistry; D011487:Protein Conformation; D047030:Sericins / Q000737:chemistry* / Q000648:ultrastructure; D047011:Silk / Q000737:chemistry* / Q000494:pharmacology / Q000648:ultrastructure
chemical_list: D002254:Carbonates; D047030:Sericins; D047011:Silk; D019301:Oleic Acid; C013173:osteum; C005686:sodium carbonate
keywords: Mechanical properties; Regenerated silk film; Residual sericin