title: Fluorescent turn-on sensing of bacterial lipopolysaccharide in artificial urine sample with sensitivity down to nanomolar by tetraphenylethylene based aggregation induced emission molecule.
abstract: A tetraphenylethylene based aggregation induced emission (AIE) probe, TPEPyE, bearing a positively charged pyridinium pendant was designed and synthesized. The positively charged TPEPyE can efficiently bind to the negatively charged lipopolysaccharide (LPS) through electrostatic interactions between the two oppositely charged species. As a result, upon the addition of LPS into the PBS solution of TPEPyE, this probe aggregated immediately onto the surface of LPS and resulted over 22-fold of fluorescence enhancement. TPEPyE exhibited good selectivity and high sensitivity toward LPS in PBS buffer solution and the detection limit was calculated to be 370 pM (3.7ng/mL). More notably, TPEPyE also retained good sensitivity and selectivity in artificial urine system (with much higher ionic strength) with the detection limit down to nanomolar. Moreover, this probe can also make a distinction between gram-positive bacteria Staphylococcus aureus (S. aureus) and gram-negative bacteria Escherichia coli (E. coli), making it a promising sensor for clinical monitoring of urinary tract infections.
authors.affiliation: Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, PR China.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, PR China. Electronic address: wangjg@iccas.ac.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Guanxin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, PR China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Lin
authors.affiliation: Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, PR China.
authors.forename: Guilan
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, PR China.
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, PR China; Material and Chemical Engineering Department, Pingxiang University, Pingxiang 337055, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Fan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000652:urine*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008070:Lipopolysaccharides / Q000652:urine*; D011726:Pyridinium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013203:Staphylococcal Infections / Q000652:urine*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013267:Stilbenes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008070:Lipopolysaccharides; D011726:Pyridinium Compounds; D013267:Stilbenes
keywords: Aggregation induced emission; Fluorescence turn-on sensor; Lipopolysaccharide