title: Long noncoding RNA uc.338 promotes cell proliferation through association with BMI1 in hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) is one of the most common human cancers all over the world. Increasing evidences have demonstrated that long noncoding RNAs (lncRNAs) play important roles in malignant transformation, tumor growth and metastasis in HCC. Among lncRNAs, ultraconserved RNAs (ucRNAs) containing an ultraconserved region have been report to contribute to human cancers. lncRNA ultraconserved element 338 (uc.338) was first found to be upregulated in HCC and promote cell growth. However, the exact mechanism by which uc.338 modulates cell growth remains unclear. In the present study, we demonstrated that uc.338 promotes HCC cell proliferation and induces cell cycle progression. RNA-immunoprecipitation and RNA pull-down assays showed that uc.338 associated with BMI1. We found that uc.338 promotes HCC cell proliferation and induces cell cycle progression through association with BMI1. uc.338 also modulated the transcription of CDKN1A. The oncogenic activity of uc.338 is partially due to its repression of p21. uc.338 may be a potential target for HCC therapy.
authors.affiliation: Department of Oncology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Bo
authors.affiliation: Department of Oncology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Oncology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oncology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Oncology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. anyonghuimed@126.com.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: An
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D063150:Polycomb Repressive Complex 1; D062085:RNA, Long Noncoding / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: C069077:BMI1 protein, human; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D062085:RNA, Long Noncoding; D063150:Polycomb Repressive Complex 1
keywords: BMI1; H2AK119ub1; PRC1; PRC2; RING1A; RING1B; lncRNA; p21; ucRNA