title: Molecular cloning and functional analysis of duck ubiquitin-specific protease 18 (USP18) gene.
abstract: In mammals, ubiquitin-specific protease 18 (USP18) is an interferon (IFN)-inducible gene and is a negative regulator of Toll-like receptor-mediated nuclear factor kappa B (NF-κB) activation. The role of USP18 in ducks (duUSP18) remains poorly understood. In the present study, we cloned and characterized the full-length coding sequence of duUSP18 from duck embryo fibroblasts (DEFs). In healthy ducks, duUSP18 transcripts were broadly expressed in different tissues, with higher expression levels in the spleen, lung and kidney. Quantitative real-time PCR (qRT-PCR) analysis revealed that duUSP18 could be induced by treatment with Poly(I:C) or LPS. Overexpression of duUSP18 inhibited NF-κB and IFN-β expression. Furthermore, deletion mutant analysis revealed that the duUSP18 region between aa 75 and 304 was essential for inhibiting NF-κB. In addition, overexpression of duUSP18 also suppressed the secretion of NF-κB-dependent proinflammatory cytokines. Taken together, these results suggest that duUSP18 regulates duck innate immune responses.
authors.affiliation: State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China; Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China; Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; College of Agricultural and Animal Husbandry, Tibet University, Linzhi, 860000, PR China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China; Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China; Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China. Electronic address: jinmeilin@mail.hzau.edu.cn.
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004372:Ducks / Q000276:immunology*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism*; D011070:Poly I-C / Q000276:immunology; D051193:Toll-Like Receptors / Q000378:metabolism; D043222:Ubiquitin Thiolesterase; D015854:Up-Regulation
chemical_list: D030161:Avian Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D051193:Toll-Like Receptors; D007371:Interferon-gamma; D010450:Endopeptidases; C485560:USP18 protein, human; D043222:Ubiquitin Thiolesterase; D011070:Poly I-C
keywords: Duck; NF-κB; USP18