title: Effect of irradiation with different laser wavelengths on oxidative stress of non-hepatectomized rats.
abstract: OBJECTIVE
  To assess the effect of two laser wavelengths, either separate or combined, on intact rat livers.
  
  
  METHODS
  Nineteen male Wistar rats (200-300 g) were submitted to laser irradiation at 5 different sites on the liver surface.Wavelengths 660 and 780 nm were used, with a dose of irradiation of 60 J/cm2/site.The animals were divided into the groups:control (C) and animals irradiated with 660 nm laser (L1), with 780 nm laser (L2) or withboth wavelengths (L3).Mitochondrial function, mitochondrial swelling, and hepatocellular malondialdehyde (MDA) levels were determined.Data were analyzed by the Mann-Whitney test, with the level of significance set at 5%.
  
  
  RESULTS
  There was a reduction of ADP-activated respiration (state 3) in group L1 compared to group C (p=0.0016), whereas the values of group L2 were similar to control.Group L3 also showed a reduction of state 3 (p=0.0159).There was a reduction of RCR in group L1 compared to control (p=0.0001) and to group L2 (p=0.0040).Mitochondrial swelling only differed between group L3 and control (p=0.0286).There was a increase in MDA levels in group L3 compared to control (p=0.0476) and to group L2 (p=0.0286) and in group L1 compared to group L2 (p=0.0132).
  
  
  CONCLUSIONS
  Although laser irradiation reduced mitochondrial function,it did not interfere with the hepatocellular energy status.
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Fabricia Teixeira
authors.initials: FT
authors.lastname: Mumic
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Marina Rodrigues Garcia da
authors.initials: MR
authors.lastname: Silveira
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Kelvin Henrique
authors.initials: KH
authors.lastname: Vilalva
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Maria Eliza
authors.initials: ME
authors.lastname: Jordani
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Maria Cecilia Jordani
authors.initials: MC
authors.lastname: Gomes
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Vanni
authors.affiliation: Sao Carlos Institute of Physics, USP, Brazil.
authors.forename: José Dirceu
authors.initials: JD
authors.lastname: Vollet Filho
authors.affiliation: Sao Carlos Institute of Physics, USP, Brazil.
authors.forename: Cristina
authors.initials: C
authors.lastname: Kurachi
authors.affiliation: Sao Carlos Institute of Physics, USP, Brazil.
authors.forename: Vanderlei Salvador
authors.initials: VS
authors.lastname: Bagnato
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Orlando de Castro E
authors.initials: Ode C
authors.lastname: Silva
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D054023:Lasers, Semiconductor; D008099:Liver / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008930:Mitochondria, Liver / Q000528:radiation effects*; D008933:Mitochondrial Swelling / Q000187:drug effects; D018384:Oxidative Stress / Q000528:radiation effects*; D011829:Radiation Dosage; D017208:Rats, Wistar; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D008315:Malondialdehyde