title: Joint enhancement strategy applied in ECL biosensor based on closed bipolar electrodes for the detection of PSA.
abstract: A highly sensitive electrogenerated chemiluminescence (ECL) biosensor was developed on the basis of a closed bipolar electrode (BPE) apparatus for the analysis of prostate specific antigen (PSA). Bipolar modifications bring up two different stages of enhancement on the same electrode. Anodic enhancement was conducted by modifying gold nanoparticles (Au NPs) to catalyze the anodic ECL reaction between luminol and hydrogen peroxide. Cathodic introduction of thionine tagged PSA antibody led to a further pertinently enhancement synchronized with the PSA amount variation, because the existence of thionine greatly increased the rate of electron gains on cathode, leading to the corresponding acceleration of anodic ECL reaction. The more thionine modified target molecules were introduced, the faster luminol was oxidized, the higher faraday current approached, and sensitive quantification was realized in correlation with the responsive ECL intensity differences. The quantification resulted in a good determination range between 0.1pg/mL and 0.1µg/mL. This strategy mainly took advantage of the special structure of closed BPE to realize a simultaneous amplification on both ends of BPE. Moreover, the platform had a potential of providing a multi-functional strategy for the realization of other bio-detections by simply substituting the PSA sandwich structure with other bio-structures.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
authors.forename: Xi-Cheng
authors.initials: XC
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
authors.forename: Mei-Sheng
authors.initials: MS
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: xujj@nju.edu.cn.
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D006046:Gold; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol; D008297:Male; D017430:Prostate-Specific Antigen
chemical_list: D008165:Luminol; D006046:Gold; D017430:Prostate-Specific Antigen
keywords: Bipolar electrode; Dual-amplification; Electrochemiluminescence; PSA detection