title: Sweeping of hydrophobic amines under inhomogeneous electric field and low surfactant concentration in micellar electrokinetic chromatography.
abstract: The influence of sample matrix on sample sweeping in MEKC was examined in the presented manuscript. Significant focusing effect was observed for relatively hydrophobic cationic compounds (emetine, strychnine and quinine) using high ionic strength sample matrix (900 mM H3 PO4 /720 mM Tris) which conductivity was about ninefold higher than utilized BGE. Moreover, the results were obtained using BGE composed of comparatively low surfactant concentration (10 mM SDS) and 40 mM H3 PO4 /32 mM Tris buffer solution. About 200 to 300-fold preconcentration of analytes was reached with the presented method. Basing on experimental results and computer simulation using Simul5 software, hypothetical mechanism of observed phenomenon was proposed.
authors.affiliation: Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Krzesimir
authors.initials: K
authors.lastname: Ciura
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kowalski
authors.affiliation: Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Nowakowska
authors.affiliation: Department of Biopharmacy, Faculty of Pharmacy, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Markuszewski
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bączek
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Dziomba
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003198:Computer Simulation; D004640:Emetine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D011803:Quinine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013331:Strychnine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D013501:Surface-Active Agents; D011803:Quinine; D013331:Strychnine; D004640:Emetine
keywords: Capillary electrophoresis; Computer simulation; Micellar electrokinetic chromatography; Preconcentration; Stacking