title: Rap2B promotes cell proliferation, migration and invasion in prostate cancer.
abstract: Rap2B, a member of the Ras family of small GTP-binding proteins, reportedly presents a high level of expression in various human tumors and plays a significant role in the development of tumor. However, the function of Rap2B in prostate cancer (PCa) remains unclear. We elucidated the stimulative role of Rap2B in PCa cell proliferation, migration and invasion by means of the CCK-8 cell proliferation assay, cell cycle analysis and transwell migration assay. Western blot analysis uncovered that elevated Rap2B leads to increased phosphorylation levels of FAK, suggesting that FAK-dependent pathway might be responsible for the effect of Rap2B on PCa cells migration and invasion. Inversely, FAK-specific inhibitor (PF-573228) can abort Rap2B-induced FAK phosphorylation. In vivo experiment confirmed that Rap2B positively regulated PCa growth and metastasis, as well as the expression of phosphorylated FAK. Collectively, these findings shed light on Rap2B as a potential therapeutic target for PCa.
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors.forename: Jiehui
authors.initials: J
authors.lastname: Di
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Cao
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China.|Department of Oncology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, 221002, People's Republic of China.
authors.forename: Juangjuan
authors.initials: J
authors.lastname: Tang
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors.forename: Keyu
authors.initials: K
authors.lastname: Gao
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors.forename: Zhesi
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China. jnzheng@xzmc.edu.cn.|Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical College, Xuzhou, 221002, Jiangsu, People's Republic of China. jnzheng@xzmc.edu.cn.|Center of Clinical Oncology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, People's Republic of China. jnzheng@xzmc.edu.cn.
authors.forename: Junnian
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010766:Phosphorylation; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology*; D020668:rap GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; C065562:RAP2B protein, human; D020668:rap GTP-Binding Proteins
keywords: FAK; Migration; Prostate cancer; Rap2B