title: A deletion at CMV UL54 codon 524 without co-existing resistance-associated mutation at UL97 confers resistance to ganciclovir: A case report.
abstract: Sequencing of Cytomegalovirus (CMV) genes to investigate antiviral resistance is a growing area of interest as treatment for CMV infection becomes more widely available and used. Using conventional sequencing methods, we identified a deletion in UL54 gene, del524, which conferred resistance to ganciclovir in a renal transplant recipient, in the absence of a co-existing resistance-conferring mutation in UL97 gene. This case report reinforces that both UL97 and UL54 genes should be sequenced when exploring CMV antiviral resistance as mutations have been identified in both genes independently of each other.
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, Royal Free Hospital, Pond Street, London, NW3 2QG, United Kingdom. Electronic address: cameron.green@nhs.net.
authors.forename: Cameron B
authors.initials: CB
authors.lastname: Green
authors.affiliation: Department of Nephrology, Royal Free London NHS Foundation Trust, Royal Free Hospital, Pond Street, London, NW3 2QG, United Kingdom.
authors.forename: Aisling
authors.initials: A
authors.lastname: O'Riordan
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, Royal Free Hospital, Pond Street, London, NW3 2QG, United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Griffiths
authors.affiliation: Department of Virology, Royal Free London NHS Foundation Trust, Royal Free Hospital, Pond Street, London, NW3 2QG, United Kingdom.
authors.forename: Tanzina
authors.initials: T
authors.lastname: Haque
mesh_terms: D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000188:drug therapy / Q000821:virology*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D024882:Drug Resistance, Viral; D005260:Female; D015774:Ganciclovir; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects; D008875:Middle Aged; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics
chemical_list: C109367:UL54 protein, Human herpesvirus 5; D014764:Viral Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase; D004259:DNA-Directed DNA Polymerase; D015774:Ganciclovir
keywords: Anti-viral resistance; Cytomegalovirus; Del524; Renal transplantation; UL54; UL97