title: Preparation and characterization of latex films photo-immobilized with IFN-α.
abstract: We developed a biomaterial by photo-immobilizing interferon-α (IFN-α) on the surface of latex condom films for the prevention and treatment of cervicitis, cervical cancers and diseases caused by cervical virus. The IFN-α modification by photoactive N-(4-azidobenzoyloxy) succinimide was characterized on a nano-scale by spectroscopy analysis and micro morphology. The anti-bacterial, anti-cancer, and anti-viral effects of the modified bioactive latex films were evaluated by antibacterial susceptibility testing, Gram staining, flow cytometry, immunofluorescence, and Western blotting. Our results showed that the photo-immobilized IFN-α latex films effectively inhibited the growth of both Neisseria gonorrhoeae and human cervical cancer HeLa cells. Moreover, the expression of anti-viral proteins, including P56, MxA, and 2', 5'-OAS, in the human cervical epithelial cell line NC104 was significantly increased by photo-immobilized IFN-α latex films. Taken together, these results suggest that photo-immobilized IFN-α latex films may have therapeutic effects against cervicitis, cervical cancers, and cervical virus.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China; School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Wu
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Kaikai
authors.initials: K
authors.lastname: Hu
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Wuya
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Rong
authors.initials: R
authors.lastname: You
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China; School of Life Science, South China Normal University, Guangzhou 510631, China. Electronic address: guanyq@scnu.edu.cn.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Guan
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D016898:Interferon-alpha / Q000737:chemistry* / Q000494:pharmacology; D007840:Latex / Q000737:chemistry; D008567:Membranes, Artificial; D009344:Neisseria gonorrhoeae / Q000187:drug effects
chemical_list: D016898:Interferon-alpha; D007840:Latex; D008567:Membranes, Artificial
keywords: HeLa; IFN-α; Latex film; NC104; Neisseria gonorrhoeae; Photo-immobilization