title: Evaluation of the intercept oral specimen collection device with HIV assays versus paired serum/plasma specimens.
abstract: Oral fluid has many advantages over blood-based techniques: it is less invasive, eliminates the occupational risk associated with needle stick accidents and collection can be self-administrated. Each individual test is packaged with a corresponding collection device. This study tested the suitability of the Intercept Oral Specimen Collection Device for different HIV diagnostic tests: three different rapid HIV tests and two adapted ELISAs, which were evaluated and compared with a gold standard on blood. In addition a total IgG quantification was performed to demonstrate the quality of the specimen. HIV antibodies were detected with a sensitivity of 100%, 99.3%, 98.6%, 100% and 95.7% for, DPP, OraQuick, Aware, Genscreen and Vironostika respectively using the Intercept Collection Device. Respective specificities were 100%, 100%, 99.3%, 97.3% and 100%.
authors.affiliation: Department of Clinical Science, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Beelaert
authors.affiliation: Blood Service, Belgian Red Cross-Flanders, Mechelen, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Van Heddegem
authors.affiliation: Department of Clinical Science, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Van Frankenhuijsen
authors.affiliation: Blood Service, Belgian Red Cross-Flanders, Mechelen, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Vandewalle
authors.affiliation: Blood Service, Belgian Red Cross-Flanders, Mechelen, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Compernolle
authors.affiliation: Department of Clinical Science, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Florence
authors.affiliation: Department of Clinical Science, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium. Electronic address: kfransen@itg.be.
authors.forename: K
authors.initials: K
authors.lastname: Fransen
mesh_terms: D015492:AIDS Serodiagnosis; D000328:Adult; D005260:Female; D015483:HIV Antibodies / Q000032:analysis* / Q000097:blood; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000821:virology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D055815:Young Adult
chemical_list: D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic
keywords: HIV rapid test; Intercept; Oral fluid