title: Sixteen years of evolution of human respiratory syncytial virus subgroup A in Buenos Aires, Argentina: GA2 the prevalent genotype through the years.
abstract: Human respiratory syncytial virus (HRSV) is the main viral cause of acute lower respiratory tract infections (LRTI) in children worldwide. In recent years, several preclinical trials with vaccine candidates have been reported. It is in this sense that molecular epidemiological studies become important. Understanding viral dispersion patterns before and after the implementation of a vaccine can provide insight into the effectiveness of the control strategies. In this work we analyzed the molecular epidemiology of HRSV-A over a period of sixteen years (1999-2014) in Buenos Aires. By bioinformatic tools we analyzed 169 sequences of the G glycoprotein gene from hospitalized pediatric patients with LRTI. We found that GA2 was the most prevalent genotype (73.35%). GA5 genotype co-circulated in our region until 2009 when it was no longer detected, except in 2011. The recently globally emerging ON1 lineage with a 72-nt duplication increased its frequency to become the only lineage detected in Buenos Aires in 2014. By discrete phylogeographic analysis of global ON1 strains we could determine that Panama could be the location of the MRCA dated June 20, 2010; and this lineage could be introduced in Argentina from Spain in April 2011. This analysis also showed temporary and geographical clustering of ON1 strains observed as phylogenetic clades with strains exclusively associated from a single country, nevertheless among our 44 ON1 strains from three outbreaks (2012-2014) we could also detect posterior reintroductions and circulation from United States, Cuba, South Korea, and Spain. The continuous phylogeographic analysis of one sublineage of Argentine ON1 strains allowed us to establish that there could be a local clustering of some strains even in neighborhoods. This work shows the potential of this type of bioinformatic tools in the context of a future vaccine surveillance network to trace the spread of new genetic lineages in human populations.
authors.affiliation: Virology Laboratory, Ricardo Gutiérrez Children's Hospital, Gallo 1330 2°, 1425 Ciudad Autónoma Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: viegasmariana@conicet.gov.ar.
authors.forename: M
authors.initials: M
authors.lastname: Viegas
authors.affiliation: Virology Laboratory, Ricardo Gutiérrez Children's Hospital, Gallo 1330 2°, 1425 Ciudad Autónoma Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: goyastephanie@conicet.gov.ar.
authors.forename: S
authors.initials: S
authors.lastname: Goya
authors.affiliation: Virology Laboratory, Ricardo Gutiérrez Children's Hospital, Gallo 1330 2°, 1425 Ciudad Autónoma Buenos Aires, Argentina; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), Argentina. Electronic address: asmistchenko@hotmail.com.
authors.forename: A S
authors.initials: AS
authors.lastname: Mistchenko
mesh_terms: D000349:Africa / Q000453:epidemiology; D001118:Argentina / Q000453:epidemiology; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D019143:Evolution, Molecular; D005260:Female; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D058974:Phylogeography; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000276:immunology / Q000517:prevention & control / Q000635:transmission; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013030:Spain / Q000453:epidemiology; D014760:Viral Fusion Proteins / Q000235:genetics*
chemical_list: C502275:G glycoprotein, Respiratory syncytial virus; D022261:Respiratory Syncytial Virus Vaccines; D014760:Viral Fusion Proteins
keywords: Circulation patterns; Genotypes; HRSV; ON1 genetic lineage; Phylogeography