title: Transfer RNA maturation in Chlamydomonas mitochondria, chloroplast and the nucleus by a single RNase P protein.
abstract: The maturation of tRNA precursors involves the 5' cleavage of leader sequences by an essential endonuclease called RNase P. Beyond the ancestral ribonucleoprotein (RNP) RNase P, a second type of RNase P called PRORP (protein-only RNase P) evolved in eukaryotes. The current view on the distribution of RNase P in cells is that multiple RNPs, multiple PRORPs or a combination of both, perform specialised RNase P activities in the different compartments where gene expression occurs. Here, we identify a single gene encoding PRORP in the green alga Chlamydomonas reinhardtii while no RNP is found. We show that its product, CrPRORP, is triple-localised to mitochondria, the chloroplast and the nucleus. Its downregulation results in impaired tRNA biogenesis in both organelles and the nucleus. CrPRORP, as a single-subunit RNase P for an entire organism, makes up the most compact and versatile RNase P machinery described in either prokaryotes or eukaryotes.
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Bonnard
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Gobert
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Arrivé
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France.
authors.forename: Franziska
authors.initials: F
authors.lastname: Pinker
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France.
authors.forename: Thalia
authors.initials: T
authors.lastname: Salinas-Giegé
authors.affiliation: Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France. giege@unistra.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Giegé
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D002696:Chlamydomonas / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D043262:Ribonuclease P / Q000235:genetics / Q000378:metabolism*
chemical_list: D012343:RNA, Transfer; D043262:Ribonuclease P
keywords: Chlorophyta; RNase P; evolution; protein-only RNase P; tRNA biogenesis