title: Antioxidant effect of hydroxytyrosol on human sperm quality during in vitro incubation.
abstract: The aim of the study was to evaluate the antioxidant potential of hydroxytyrosol (HT) on human sperm quality during incubation in vitro. Semen samples collected from men attending the Laboratory of Histology-Embryology of Sfax Faculty of Medicine (Tunisia) for infertility investigations were evaluated for initial sperm parameters. Only normal selected ejaculates (n = 15) were centrifuged and incubated further with or without HT (200ug ml-1) at room temperature for 45 min. After incubation, sperm motility and viability, DNA oxidation and reactive oxygen species (ROS) production were assessed. The results showed that centrifugation significantly influenced sperm motility and viability. The supplementation of HT in incubating media improved (P = 0.01) significantly sperm viability and decreased sperm DNA oxidation (P < 0.001) and ROS levels (P = 0.03) following centrifugation. It can be concluded that supplementation of HT might be helpful to maintain the human spermatozoon after centrifugation.
authors.affiliation: Laboratory of Histology-Embryology, Medicine Faculty of Sfax, Sfax, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Kedechi
authors.affiliation: Laboratory of Histology-Embryology, Medicine Faculty of Sfax, Sfax, Tunisia.|Laboratory of Human Molecular Genetics, Medicine Faculty of Sfax, Sfax, Tunisia.
authors.forename: N
authors.initials: N
authors.lastname: Zribi
authors.affiliation: Regional Center of Blood Transfusion of Sfax, Sfax, Tunisia.
authors.forename: N
authors.initials: N
authors.lastname: Louati
authors.affiliation: Regional Center of Blood Transfusion of Sfax, Sfax, Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Menif
authors.affiliation: Laboratory of Histology-Embryology, Medicine Faculty of Sfax, Sfax, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Sellami
authors.affiliation: Higher Institut of Biotechnology of Sfax, Sfax, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Lassoued
authors.affiliation: Higher Institut of Biotechnology of Sfax, Sfax, Tunisia.
authors.forename: R
authors.initials: R
authors.lastname: Ben Mansour
authors.affiliation: Laboratory of Histology-Embryology, Medicine Faculty of Sfax, Sfax, Tunisia.|Laboratory of Human Molecular Genetics, Medicine Faculty of Sfax, Sfax, Tunisia.
authors.forename: L
authors.initials: L
authors.lastname: Keskes
authors.affiliation: Laboratory of Histology-Embryology, Medicine Faculty of Sfax, Sfax, Tunisia.
authors.forename: T
authors.initials: T
authors.lastname: Rebai
authors.affiliation: Laboratory of Histology-Embryology, Medicine Faculty of Sfax, Sfax, Tunisia.
authors.forename: N
authors.initials: N
authors.lastname: Chakroun
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D055101:Semen Analysis; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; C005975:3,4-dihydroxyphenylethanol; D010626:Phenylethyl Alcohol
keywords: Antioxidants; hydroxytyrosol; oxidative stress; sperm