title: Proteomic profiling of lymphedema development in mouse model.
abstract: The lymphatic vascular system plays an important role in tissue fluid homeostasis. Lymphedema is a chronic, progressive, and incurable condition that leads to lymphatic fluid retention; it may be primary (heritable) or secondary (acquired) in nature. Although there is a growing understanding of lymphedema, methods for the prevention and treatment of lymphedema are still limited. In this study, we investigated differential protein expressions in sham-operated and lymphedema-operated mice for 3 days, using two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. Male improved methodology for culturing noninbred (ICR) mice developed lymphedema in the right hindlimb. Twenty functional proteins were found to be differentially expressed between lymphedema induced-right leg tissue and normal left leg tissue. Out of these proteins, the protein levels of apolipoprotein A-1 preprotein, alpha-actinin-3, mCG21744, parkinson disease, serum amyloid P-component precursor, annexin A8, mKIAA0098 protein, and fibrinogen beta chain precursor were differentially upregulated in the lymphedema mice compared with the sham-operated group. Western blotting analysis was used to validate the proteomics results. Our results showing differential up-regulation of serum amyloid P-component precursor, parkinson disease, and apolipoprotein A-1 preprotein in lymphedema model over sham-operated model suggest important insights into pathophysiological target for lymphedema. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of food and nutrition, College of Natural Science, Chosun University, Gwangju, Republic of Korea.
authors.forename: Joomin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.|Yeom's Clinic of Palliative Medicine, Seoul, Republic of Korea.
authors.forename: Haeun
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Kangsan
authors.initials: K
authors.lastname: Roh
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Sungrae
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
authors.affiliation: Yeom's Clinic of Palliative Medicine, Seoul, Republic of Korea.
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Yeom
authors.affiliation: Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.
authors.forename: Seyeon
authors.initials: S
authors.lastname: Park
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D006614:Hindlimb / Q000473:pathology; D008209:Lymphedema / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008813:Mice, Inbred ICR; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D020543:Proteome
keywords: development; lymphedema; mouse model; proteomics; serum amyloid P-component precursor