title: Transfer of fibroblast sheets cultured on thermoresponsive dishes with membranes.
abstract: In cell or tissue engineering, it is essential to develop a support for cell-to-cell adhesion, which leads to the generation of cell sheets connected by extracellular matrix. Such supports must be hydrophobic and should result in a detachable cell sheet. A thermoresponsive support that enables the cultured cell sheet to detach using only a change in temperature could be an interesting alternative in regenerative medicine. The aim of this study was to evaluate plates covered with thermoresponsive polymers as supports for the formation of fibroblast sheets and to develop a damage-free procedure for cell sheet transfer with the use of membranes as transfer tools. Human skin fibroblasts were seeded on supports coated with a thermoresponsive polymer: commercial UpCell™ dishes (NUNC™) coated with thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) and dishes coated with thermoresponsive poly(tri(ethylene glycol) monoethyl ether methacrylate) (P(TEGMA-EE)). Confluent fibroblast sheets were effectively cultured and harvested from both commercial PNIPAM-coated dishes and laboratory P(TEGMA-EE)-coated dishes. To transfer a detached cell sheet, two membranes, Immobilon-P(®) and SUPRATHEL(®), were examined. The use of SUPRATHEL for relocating the cell sheets opens a new possibility for the clinical treatment of wounds. This study established the background for implementing thermoresponsive supports for transplanting in vitro cultured fibroblasts.
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland.|Faculty of Health Sciences, University of Bielsko-Biala, Willowa 2, 43-309, Bielsko-Biała, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Kawecki
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kraut
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Klama-Baryła
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Łabuś
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland. diana.hoff.lenczewska@gmail.com.
authors.forename: Diana
authors.initials: D
authors.lastname: Kitala
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Nowak
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Glik
authors.affiliation: Dr Stanislaw Sakiel Centre for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland.|Department of Molecular Biology and Genetics, Medical University of Silesia, Medyków 18, 40-752, Katowice, Poland.
authors.forename: Aleksander L
authors.initials: AL
authors.lastname: Sieroń
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819, Zabrze, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Utrata-Wesołek
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819, Zabrze, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Trzebicka
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819, Zabrze, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dworak
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819, Zabrze, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Szweda
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D008567:Membranes, Artificial; D012867:Skin / Q000166:cytology; D013696:Temperature; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
chemical_list: D008567:Membranes, Artificial