title: Bioaccumulation of hexachlorobenzene in the terrestrial isopod Porcellio scaber.
abstract: A test system to investigate the biomagnification of organic chemicals in the terrestrial isopod Porcellio scaber was developed and validated. Adult isopods were fed on alder leaf powder (Alnus glutinosa) spiked with [14C]hexachlorobenzene (HCB). Test animals, sampled regularly during the uptake (16 d) and depuration phases (16 d), were analyzed, and the kinetics of tissue concentrations were determined. Uptake (k1) and depuration rates (k2) were calculated to estimate kinetic biomagnification factors (BMFs). In addition, the effect of coprophagy on the uptake and accumulation of HCB as well as the tissue distribution of HCB in P. scaber was investigated. The test system was shown to be suitable for investigations into the terrestrial bioaccumulation of chemicals. Coprophagy had no effect on the bioaccumulation of HCB in P. scaber. The hepatopancreas was identified as the main target tissue for HCB accumulation. The low BMF of 0.057 resulted from an assimilation efficiency (α) of 31.42%, a low uptake rate k1(0.009 d-1), and a high depuration rate k2(0.164 d-1). The results indicate that the terrestrial bioaccumulation of organic chemicals in P. scaber might not represent a worst-case scenario for biomagnification, limiting the value of the test system for the regulatory assessment of organic chemicals. Environ Toxicol Chem 2016;35:2867-2873. © 2016 The Authors. Environmental Toxicology and Chemistry Published by Wiley Periodicals, Inc. on behalf of SETAC.
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Schmallenberg, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kampe
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Schmallenberg, Germany. christian.schlechtriem@ime.fraunhofer.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Schlechtriem
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D043143:Hepatopancreas / Q000187:drug effects* / Q000378:metabolism; D006581:Hexachlorobenzene / Q000378:metabolism / Q000633:toxicity*; D033321:Isopoda / Q000187:drug effects* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D004785:Environmental Pollutants; D006581:Hexachlorobenzene
keywords: Biomagnification; Coprophagy; Terrestrial isopods; Tissue distribution; [14C]hexachlorobenzene