title: Biosensors for the Diagnosis of Celiac Disease: Current Status and Future Perspectives.
abstract: Celiac disease (CD) is an autoimmune enteropathy initiated and sustained by the ingestion of gluten in genetically susceptible individuals. It is caused by a dysregulated immune response toward both dietary antigens, the gluten proteins of wheat, rye, and barley, and autoantigens, the enzyme tissue transglutaminase (TG2). The small intestine is the target organ. Although routine immunochemical protocols for a laboratory diagnosis of CD are available, faster, easier-to-use, and cheaper analytical devices for CD diagnosis are currently unavailable. This review focuses on biosensors, consisting of a physicochemical transducer and a bioreceptor, as promising analytical tools for diagnosis of CD and other diseases. Examples of recently developed biosensors as well as expectations for future lines of research and development in this field are presented.
authors.affiliation: German Research Centre for Food Chemistry, Leibniz Institut, Freising, Germany.
authors.forename: Katharina Anne
authors.initials: KA
authors.lastname: Scherf
authors.affiliation: Clinica Medica I, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy.
authors.forename: Rachele
authors.initials: R
authors.lastname: Ciccocioppo
authors.affiliation: Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pohanka
authors.affiliation: Department of Public Health and Hygiene, Faculty of Medicine, Pavol Jozef Safarik University, Kosice, Slovakia.
authors.forename: Kvetoslava
authors.initials: K
authors.lastname: Rimarova
authors.affiliation: Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
authors.forename: Radka
authors.initials: R
authors.lastname: Opatrilova
authors.affiliation: Department of Gastroenterology, Central University Hospital of Asturias (HUCA), Oviedo, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rodrigo
authors.affiliation: Laboratory of Structural Biology and Proteomics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho tr 1946/1, 612 42, Brno, Czech Republic. kruzliakpeter@gmail.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Kruzliak
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology; D055664:Electrochemical Techniques; D019204:GTP-Binding Proteins / Q000276:immunology; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000276:immunology
chemical_list: D000906:Antibodies; D005903:Gliadin; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins
keywords: Antibody; Biosensors; Celiac disease; Diagnosis; ELISA; Immunochemistry