title: Regulatory T Cells Contribute to the Inhibition of Radiation-Induced Acute Lung Inflammation via Bee Venom Phospholipase A₂ in Mice.
abstract: Bee venom has long been used to treat various inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Previously, we reported that bee venom phospholipase A₂ (bvPLA₂) has an anti-inflammatory effect through the induction of regulatory T cells. Radiotherapy is a common anti-cancer method, but often causes adverse effects, such as inflammation. This study was conducted to evaluate the protective effects of bvPLA₂ in radiation-induced acute lung inflammation. Mice were focally irradiated with 75 Gy of X-rays in the lung and administered bvPLA₂ six times after radiation. To evaluate the level of inflammation, the number of immune cells, mRNA level of inflammatory cytokine, and histological changes in the lung were measured. BvPLA₂ treatment reduced the accumulation of immune cells, such as macrophages, neutrophils, lymphocytes, and eosinophils. In addition, bvPLA₂ treatment decreased inflammasome-, chemokine-, cytokine- and fibrosis-related genes' mRNA expression. The histological results also demonstrated the attenuating effect of bvPLA₂ on radiation-induced lung inflammation. Furthermore, regulatory T cell depletion abolished the therapeutic effects of bvPLA₂ in radiation-induced pneumonitis, implicating the anti-inflammatory effects of bvPLA₂ are dependent upon regulatory T cells. These results support the therapeutic potential of bvPLA₂ in radiation pneumonitis and fibrosis treatments.
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South Korea. ssd060@naver.com.
authors.forename: Dasom
authors.initials: D
authors.lastname: Shin
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South Korea. glee@khu.ac.kr.
authors.forename: Gihyun
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Radiation Oncology, Yonsei University College of Medicine, Seoul 120-752, South Korea. 19980769@hanmail.net.
authors.forename: Sung-Hwa
authors.initials: SH
authors.lastname: Sohn
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South Korea. ih_sj@naver.com.
authors.forename: Soojin
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South Korea. jhkh242@naver.com.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Jung
authors.affiliation: Department of Radiation Oncology, Yonsei University College of Medicine, Seoul 120-752, South Korea. carol114@yuhs.ac.
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South Korea. yje2020@hanmail.net.
authors.forename: Jieun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, Yonsei University College of Medicine, Seoul 120-752, South Korea. jjhmd@yuhs.ac.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Cho
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, South Korea. hbae@khu.ac.kr.
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001514:Bee Venoms / Q000201:enzymology*; D005260:Female; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology / Q000528:radiation effects; D008810:Mice, Inbred C57BL; D054467:Phospholipases A2 / Q000494:pharmacology / Q000627:therapeutic use*; D017564:Radiation Pneumonitis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D001514:Bee Venoms; D054467:Phospholipases A2
keywords: bee venom; inflammation; phospholipase A2; radiotherapy; regulatory T cells