title: A putative amino acid transporter determines sensitivity to the two-peptide bacteriocin plantaricin JK.
abstract: Lactobacillus plantarum produces a number of antimicrobial peptides (bacteriocins) that mostly target closely related bacteria. Although bacteriocins are important for the ecology of these bacteria, very little is known about how the peptides target sensitive cells. In this work, a putative membrane protein receptor of the two-peptide bacteriocin plantaricin JK was identified by comparing Illumina sequence reads from plantaricin JK-resistant mutants to a crude assembly of the sensitive wild-type Weissella viridescens genome using the polymorphism discovery tool VAAL. Ten resistant mutants harbored altogether seven independent mutations in a gene encoding an APC superfamily protein with 12 transmembrane helices. The APC superfamily transporter thus is likely to serve as a target for plantaricin JK on sensitive cells.
authors.affiliation: Biochemistry and Molecular Biology Section, Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, Oslo, 0316, Norway.
authors.forename: Camilla
authors.initials: C
authors.lastname: Oppegård
authors.affiliation: Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Nijenborgh 7 9747 AG, Groningen, The Netherlands.
authors.forename: Morten
authors.initials: M
authors.lastname: Kjos
authors.affiliation: Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Nijenborgh 7 9747 AG, Groningen, The Netherlands.
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: Veening
authors.affiliation: Biochemistry and Molecular Biology Section, Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, Oslo, 0316, Norway.
authors.forename: Jon
authors.initials: J
authors.lastname: Nissen-Meyer
authors.affiliation: Biochemistry and Molecular Biology Section, Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, Oslo, 0316, Norway.
authors.forename: Tom
authors.identifier: 0000-0002-2131-4469
authors.initials: T
authors.lastname: Kristensen
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D017422:Sequence Analysis, DNA; D058836:Weissella / Q000187:drug effects* / Q000235:genetics*
chemical_list: D001430:Bacteriocins; C117245:plantaricin JK
keywords: Antibacterial activity; bacteriocins; membrane proteins; mode of action