title: Oxidative release of natural glycans for functional glycomics.
abstract: Glycans have essential roles in biology and the etiology of many diseases. A major hurdle in studying glycans through functional glycomics is the lack of methods to release glycans from diverse types of biological samples. Here we describe an oxidative strategy using household bleach to release all types of free reducing N-glycans and O-glycan-acids from glycoproteins, and glycan nitriles from glycosphingolipids. Released glycans are directly useful in glycomic analyses and can be derivatized fluorescently for functional glycomics. This chemical method overcomes the limitations in glycan generation and promotes archiving and characterization of human and animal glycomes and their functions.
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Ju
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lasanajak
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Kudelka
authors.affiliation: Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004531:Eggs / Q000032:analysis; D005260:Female; D041981:Gastrointestinal Tract / Q000737:chemistry; D054794:Glycomics / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry; D008810:Mice, Inbred C57BL; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D037121:Plant Lectins / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry*; D012973:Sodium Hypochlorite / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006023:Glycoproteins; D006028:Glycosphingolipids; D016877:Oxidants; D037121:Plant Lectins; D011134:Polysaccharides; D012973:Sodium Hypochlorite