title: FAK Kinase Activity Is Required for the Progression of c-MET/β-Catenin-Driven Hepataocellular Carcinoma.
abstract: There is an urgent need to develop new and more effective therapeutic strategies and agents to treat hepatocellular carcinoma (HCC). We have recently found that deletion of Fak in hepatocytes before tumors form inhibits tumor development and prolongs survival of animals in a c-MET (MET)/β-catenin (CAT)-driven HCC mouse model. However, it has yet to be determined whether FAK expression in hepatocytes promotes MET/CAT-induced HCC progression after tumor initiation. In addition, it remains unclear whether FAK promotes HCC development through its kinase activity. We generated hepatocyte-specific inducible Fak-deficient mice (Alb-creERT2; Fak(flox/flox)) to examine the role of FAK in HCC progression. We reexpressed wild-type and mutant FAK in Fak-deficient mice to determine FAK's kinase activity in HCC development. We also examined the efficacy of a FAK kinase inhibitor PF-562271 on HCC inhibition. We found that deletion of Fak after tumors form significantly repressed MET/CAT-induced tumor progression. Ectopic FAK expression restored HCC formation in hepatocyte-specific Fak-deficient mice. However, overexpression of a FAK kinase-dead mutant led to reduced tumor load compared to mice that express wild-type FAK. Furthermore, PF-562271 significantly suppressed progression of MET/CAT-induced HCC. Fak kinase activity is important for MET/CAT-induced HCC progression. Inhibiting FAK kinase activity provides a potential therapeutic strategy to treat HCC.
authors.affiliation: Department of Surgery and Oncology Institute, Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA.
authors.forename: Na
authors.initials: N
authors.lastname: Shang
authors.forename: Maribel
authors.initials: M
authors.lastname: Arteaga
authors.forename: Ali
authors.initials: A
authors.lastname: Zaidi
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Cotler
authors.forename: Peter
authors.initials: P
authors.lastname: Breslin
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Ding
authors.forename: Paul
authors.initials: P
authors.lastname: Kuo
authors.forename: Michael
authors.initials: M
authors.lastname: Nishimura
authors.forename: Jiwang
authors.initials: J
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D010766:Phosphorylation / Q000502:physiology; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051176:beta Catenin; D019859:Proto-Oncogene Proteins c-met; D051417:Focal Adhesion Kinase 1