title: Genome Sequencing and Comparative Analysis of Saccharomyces cerevisiae Strains of the Peterhof Genetic Collection.
abstract: The Peterhof genetic collection of Saccharomyces cerevisiae strains (PGC) is a large laboratory stock that has accumulated several thousands of strains for over than half a century. It originated independently of other common laboratory stocks from a distillery lineage (race XII). Several PGC strains have been extensively used in certain fields of yeast research but their genomes have not been thoroughly explored yet. Here we employed whole genome sequencing to characterize five selected PGC strains including one of the closest to the progenitor, 15V-P4, and several strains that have been used to study translation termination and prions in yeast (25-25-2V-P3982, 1B-D1606, 74-D694, and 6P-33G-D373). The genetic distance between the PGC progenitor and S288C is comparable to that between two geographically isolated populations. The PGC seems to be closer to two bakery strains than to S288C-related laboratory stocks or European wine strains. In genomes of the PGC strains, we found several loci which are absent from the S288C genome; 15V-P4 harbors a rare combination of the gene cluster characteristic for wine strains and the RTM1 cluster. We closely examined known and previously uncharacterized gene variants of particular strains and were able to establish the molecular basis for known phenotypes including phenylalanine auxotrophy, clumping behavior and galactose utilization. Finally, we made sequencing data and results of the analysis available for the yeast community. Our data widen the knowledge about genetic variation between Saccharomyces cerevisiae strains and can form the basis for planning future work in PGC-related strains and with PGC-derived alleles.
authors.affiliation: Dept. of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russia.|Bioinformatics Institute, St. Petersburg, Russia.
authors.forename: Polina B
authors.initials: PB
authors.lastname: Drozdova
authors.affiliation: Dept. of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russia.|St. Petersburg Scientific Center of RAS, St. Petersburg, Russia.
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Tarasov
authors.affiliation: St. Petersburg Branch, Vavilov Institute of General Genetics of the Russian Academy of Sciences, St. Petersburg, Russia.|Dept. of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russia.|Laboratory of Amyloid Biology, Saint Petersburg State University, St. Petersburg, Russia.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Matveenko
authors.affiliation: Dept. of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russia.|Bioinformatics Institute, St. Petersburg, Russia.
authors.forename: Elina A
authors.initials: EA
authors.lastname: Radchenko
authors.affiliation: St. Petersburg Branch, Vavilov Institute of General Genetics of the Russian Academy of Sciences, St. Petersburg, Russia.|Institute of Translational Biomedicine, Saint Petersburg State University, St. Petersburg, Russia.|Dept. of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russia.
authors.forename: Julia V
authors.initials: JV
authors.lastname: Sopova
authors.affiliation: Research Resource Center for Molecular and Cell Technologies, Research Park, Saint-Petersburg State University, St. Petersburg, Russia.
authors.forename: Dmitrii E
authors.initials: DE
authors.lastname: Polev
authors.affiliation: St. Petersburg Branch, Vavilov Institute of General Genetics of the Russian Academy of Sciences, St. Petersburg, Russia.|Dept. of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russia.
authors.forename: Sergey G
authors.initials: SG
authors.lastname: Inge-Vechtomov
authors.affiliation: Theodosius Dobzhansky Center for Genome Bioinformatics, Saint Petersburg State University, St. Petersburg, Russia.|Bioinformatics Institute, St. Petersburg, Russia.
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Dobrynin
mesh_terms: D056915:DNA Copy Number Variations; D016681:Genome, Fungal; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics*