title: Backbone and side-chain (1)H, (13)C, and (15)N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C.
abstract: The apo-form of the 23.3 kDa catalytic domain of the AA9 family lytic polysaccharide monooxygenase NcLPMO9C from Neurospora crassa has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the (1)H, (13)C, and (15)N chemical shift assignments of this LPMO.
authors.affiliation: NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Sælands vei 6/8, 7491, Trondheim, Norway.
authors.forename: Gaston
authors.initials: G
authors.lastname: Courtade
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Frederik Bajers vej 7H, 9220, Aalborg Ø, Denmark.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wimmer
authors.affiliation: Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Dimarogona
authors.affiliation: Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Sandgren
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432, Ås, Norway.
authors.forename: Vincent G H
authors.initials: VG
authors.lastname: Eijsink
authors.affiliation: NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Sælands vei 6/8, 7491, Trondheim, Norway. finn.l.aachmann@ntnu.no.
authors.forename: Finn L
authors.initials: FL
authors.lastname: Aachmann
mesh_terms: D001051:Apoenzymes / Q000737:chemistry* / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000378:metabolism*
chemical_list: D001051:Apoenzymes; D011134:Polysaccharides; D006899:Mixed Function Oxygenases
keywords: AA9; Cellulose; LPMO; Lytic polysaccharide monooxygenase; Xyloglucan