title: SharpViSu: integrated analysis and segmentation of super-resolution microscopy data.
abstract: We introduce SharpViSu, an interactive open-source software with a graphical user interface, which allows performing processing steps for localization data in an integrated manner. This includes common features and new tools such as correction of chromatic aberrations, drift correction based on iterative cross-correlation calculations, selection of localization events, reconstruction of 2D and 3D datasets in different representations, estimation of resolution by Fourier ring correlation, clustering analysis based on Voronoi diagrams and Ripley's functions. SharpViSu is optimized to work with eventlist tables exported from most popular localization software. We show applications of these on single and double-labelled super-resolution data.
  
  
  
  SharpViSu is available as open source code and as compiled stand-alone application under https://github.com/andronovl/SharpViSu
  
  
  
  klaholz@igbmc.fr
  
  
  
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), Illkirch, France Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France Université de Strasbourg, Strasbourg, France.
authors.forename: Leonid
authors.initials: L
authors.lastname: Andronov
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), Illkirch, France Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France Université de Strasbourg, Strasbourg, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Lutz
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), Illkirch, France Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France Université de Strasbourg, Strasbourg, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vonesch
authors.affiliation: Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), Illkirch, France Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France Institut National de la Santé et de la Recherche Médicale (INSERM) U964, Illkirch, France Université de Strasbourg, Strasbourg, France.
authors.forename: Bruno P
authors.initials: BP
authors.lastname: Klaholz
mesh_terms: D003196:Computer Graphics; D007091:Image Processing, Computer-Assisted; D008853:Microscopy; D012984:Software; D014584:User-Computer Interface