title: In vitro CRISPR-Cas9-mediated efficient Ad5 vector modification.
abstract: The CRISPR-Cas9 genome editing system has been widely used in multiple cells and organisms. Here we developed a CRISPR-Cas9 based in vitro large DNA vector editing system, using the Ad5-based vector as an example. We demonstrate use of this system to generate targeted mutations, in-frame gene deletion, and gene replacement. This in vitro CRISPR editing system exhibits high efficiency and accuracy. We believe this system can be applied in a variety of experimental settings.
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China. Electronic address: 1212qq-@163.com.
authors.forename: Lichun
authors.initials: L
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China.
authors.forename: Pumin
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D064113:CRISPR-Cas Systems / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D014162:Transfection / Q000379:methods*
keywords: Ad5/F35; Adenovirus; CRISPR-Cas9; Fiber modification