title: A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber.
abstract: High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F2 populations of S1000 × S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on seven chromosomes, and spanned 1061.19 cM. The average genetic distance is 0.35 cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Zhu
authors.affiliation: Biomarker Technologies Corporation Beijing, China.
authors.forename: Long
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Jian-Tao
authors.initials: JT
authors.lastname: Yang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Jia-Ni
authors.initials: JN
authors.lastname: Wu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Qu
authors.affiliation: Shanghai Seed Management Station Shanghai, China.
authors.forename: Dan-Qing
authors.initials: DQ
authors.lastname: Yao
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Guo
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Lian
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Huan-Le
authors.initials: HL
authors.lastname: He
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Pan
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.
authors.forename: Run
authors.initials: R
authors.lastname: Cai
keywords: Cucumis sativus L.; QTL analysis; SLAF-seq; SNPs; high-density genetic map