title: Nitrogen removal characteristics of indigenous aerobic denitrifiers and changes in the microbial community of a reservoir enclosure system via in situ oxygen enhancement using water lifting and aeration technology.
abstract: Indigenous aerobic denitrifiers of a reservoir system were enhanced in situ by water lifting and aeration technology. Nitrogen removal characteristics and changes in the bacterial community were investigated. Results from a 30-day experiment showed that the TN in the enhanced water system decreased from 1.08-2.02 to 0.75-0.91mg/L and that TN removal rates varied between 21.74% and 52.54% without nitrite accumulation, and TN removal rate of surface sediments reached 41.37±1.55%. The densities of aerobic denitrifiers in the enhanced system increased. Furthermore, the enhanced system showed a clear inhibition of Fe, Mn, and P performances. Community analysis using Miseq showed that diversity was higher in the in situ oxygen enhanced system than in the control system. In addition, the microbial composition was significantly different between systems. It can be concluded that in situ enhancement of indigenous aerobic denitrifiers is very effective in removing nitrogen from water reservoir systems.
authors.affiliation: Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: huangtinglin@xauat.edu.cn.
authors.forename: Tinglin
authors.initials: T
authors.lastname: Huang
authors.affiliation: School of Civil and Environmental Engineering, Faculty of Engineering and Information Technology, University of Technology Sydney, NSW 2007, Australia.
authors.forename: Huu Hao
authors.initials: HH
authors.lastname: Ngo
authors.affiliation: Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Haihan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Mingzheng
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Shi
authors.affiliation: Key Lab of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000332:Aerobiosis; D000641:Ammonia / Q000302:isolation & purification; D058440:Denitrification; D005798:Genes, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D060106:Lakes / Q000382:microbiology; D059013:Microbial Consortia / Q000235:genetics; D009566:Nitrates / Q000302:isolation & purification; D009573:Nitrites / Q000302:isolation & purification; D010100:Oxygen / Q000737:chemistry; D062065:Wastewater / Q000032:analysis* / Q000382:microbiology; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D000641:Ammonia; D009566:Nitrates; D009573:Nitrites; D010100:Oxygen; D062065:Wastewater
keywords: Aerobic denitrification; In situ; Miseq high-throughput sequencing technique; Nitrogen removal; Water reservoir