title: Utilization of Paneer Whey Waste for Cost-Effective Production of Rhamnolipid Biosurfactant.
abstract: The present study aimed at isolating rhamnolipid biosurfactant-producing bacteria that could utilize paneer whey, an abundant waste source as sole medium for the production purpose. Pseudomonas aeruginosa strain, SR17, was isolated from hydrocarbon-contaminated soil that could efficiently utilize paneer whey for rhamnolipid production and reduce surface tension of the medium from 52 to 26.5 mN/m. The yield of biosurfactant obtained was 2.7 g/l, upgraded to 4.8 g/l when supplemented with 2 % glucose and mineral salts. Biochemical, FTIR, and LC-MS analysis revealed that extracted biosurfactant is a combination of both mono and di-rhamnolipid congeners. The critical micelle concentration (CMC) was measured to be 110 mg/l. Emulsification activity of the biosurfactant against n-hexadecane, olive oil, kerosene, diesel oil, engine oil, and crude oil were found to be 83, 88, 81, 92, 86, and 100 %, respectively. The rhamnolipid was detected to be non-toxic against mouse fibroblastic cell line L292.
authors.affiliation: Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science & Technology (IASST), Paschim Boragaon, Guwahati, 781 035, Assam, India.
authors.forename: Rupshikha
authors.initials: R
authors.lastname: Patowary
authors.affiliation: Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science & Technology (IASST), Paschim Boragaon, Guwahati, 781 035, Assam, India.
authors.forename: Kaustuvmani
authors.initials: K
authors.lastname: Patowary
authors.affiliation: Department of Biotechnology, Gauhati University, Guwahati, 781014, India.
authors.forename: Mohan Chandra
authors.initials: MC
authors.lastname: Kalita
authors.affiliation: Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science & Technology (IASST), Paschim Boragaon, Guwahati, 781 035, Assam, India. sureshdeka@gmail.com.
authors.forename: Suresh
authors.initials: S
authors.lastname: Deka
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D001709:Biotechnology / Q000191:economics* / Q000379:methods*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002611:Cheese; D002853:Chromatography, Liquid; D003362:Cost-Benefit Analysis; D004655:Emulsions / Q000737:chemistry; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D013058:Mass Spectrometry; D051379:Mice; D008823:Micelles; D010802:Phylogeny; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D014866:Waste Products; D000067796:Whey / Q000737:chemistry*
chemical_list: D004655:Emulsions; D006017:Glycolipids; D008823:Micelles; D013501:Surface-Active Agents; D014866:Waste Products; C418382:rhamnolipid
keywords: Critical micelle concentration; Emulsification; Non-toxic; Paneer whey; Pseudomonas aeruginosa SR17; Rhamnolipid