title: Establishment of a new cell line from the snout tissue of golden pompano Trachinotus ovatus, and its application in virus susceptibility.
abstract: A new marine-fish cell line, designated GPS, was established from the snout tissue of golden pompano Trachinotus ovatus. GPS cells multiplied well in Leibovitz's L-15 containing 10% foetal bovine serum (FBS) at 28° C and the cells have been subcultured for >60 passages. Polymerase chain reaction (PCR) amplification of 16S ribosomal (r)RNA confirmed the origin of this cell line from T. ovatus. Chromosome analysis showed that GPS cells exhibited chromosomal aneuploidy with a modal chromosome number of 54. Bright green fluorescence signal was observed in enhanced green fluorescent protein (EGFP)-N3 transfected cells, indicating that GPS cells could be used to investigate gene functions in vitro. The GPS cells were highly susceptible to Singapore grouper iridovirus (SGIV), which was demonstrated by the presence of severe cytopathic effect (CPE) and increased viral titres. Real-time quantitative PCR and Western blot analysis showed that the viral gene transcription and protein synthesis occurred during SGIV infection in GPS cells. Thus, this study described the characteristic of a new cell line from the snout tissue of T. ovatus that could be used as a tool for propagation of iridovirus and genetic manipulation to investigate host-pathogen interactions.
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: S
authors.initials: S
authors.lastname: Wei
authors.affiliation: College of Ecological Environment Engineering, Qinghai University, Xining, 810016, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: X
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang, 524088, China.
authors.forename: J
authors.initials: J
authors.lastname: Cai
authors.affiliation: College of Ecological Environment Engineering, Qinghai University, Xining, 810016, China.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.|College of Marine Sciences, South China Agricultural University, Guangzhou, 510641, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Qin
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002460:Cell Line; D002875:Chromosomes; D004198:Disease Susceptibility; D005399:Fishes / Q000235:genetics / Q000821:virology*; D049452:Green Fluorescent Proteins / Q000032:analysis; D054884:Host-Pathogen Interactions; D017994:Iridovirus / Q000502:physiology*; D014162:Transfection; D014776:Virus Cultivation
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: cell line; snout; transgenic manipulation; virus infection