title: Identification and Characterization of a New Pecan [Carya illinoinensis (Wangenh.) K. Koch] Allergen, Car i 2.
abstract: The 7S vicilin and 11S legumin seed storage globulins belong to the cupin protein superfamily and are major food allergens in many foods from the "big eight" food allergen groups. Here, for the first time, pecan vicilin was found to be a food allergen. Western blot experiments revealed that 30% of 27 sera used in this study and 24% of the sera from 25 patients with double-blind, placebo controlled clinical pecan allergy contained IgE antibodies specific to pecan vicilin. This allergen consists of a low-complexity region at its N-terminal and a structured domain at the C-terminal that contains two cupin motifs and forms homotrimers. The crystal structure of recombinant pecan vicilin was determined. The refined structure gave R/Rfree values of 0.218/0.262 for all data to 2.65 Å. There were two trimeric biological units in the crystallographic asymmetric unit. Pecan vicilin is also a copper protein. These data may facilitate the understanding of the nutritional value and the allergenicity relevance of the copper binding property of seed storage proteins in tree nuts.
authors.affiliation: Western Regional Research Center, Pacific West Area, Agricultural Research Service, U.S. Department of Agriculture , 800 Buchanan Street, Albany, California 94710, United States.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Western Regional Research Center, Pacific West Area, Agricultural Research Service, U.S. Department of Agriculture , 800 Buchanan Street, Albany, California 94710, United States.
authors.forename: BoRam
authors.initials: B
authors.lastname: Lee
authors.affiliation: Western Regional Research Center, Pacific West Area, Agricultural Research Service, U.S. Department of Agriculture , 800 Buchanan Street, Albany, California 94710, United States.
authors.forename: Wen-Xian
authors.initials: WX
authors.lastname: Du
authors.affiliation: Division of Pediatric Immunology, Allergy, and Rheumatology, Department of Pediatrics, Stanford University School of Medicine , 269 Campus Drive, Stanford, California 94305, United States.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Lyu
authors.affiliation: Division of Pediatric Immunology, Allergy, and Rheumatology, Department of Pediatrics, Stanford University School of Medicine , 269 Campus Drive, Stanford, California 94305, United States.
authors.forename: Kari C
authors.initials: KC
authors.lastname: Nadeau
authors.affiliation: Crop Germplasm Research, Southern Plains Agricultural Research Center, USDA-ARS-SPA , 2881 F&B Road, College Station, Texas 77845, United States.
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Grauke
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology , Tianjin, 300222, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology , Tianjin, 300222, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Western Regional Research Center, Pacific West Area, Agricultural Research Service, U.S. Department of Agriculture , 800 Buchanan Street, Albany, California 94710, United States.|School of Food Science and Technology, Jiangnan University , 214122, Wuxi, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Fan
authors.affiliation: College of Chemistry and Environmental Engineering, Shenzhen University , Shenzhen 518060, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yi
authors.affiliation: Western Regional Research Center, Pacific West Area, Agricultural Research Service, U.S. Department of Agriculture , 800 Buchanan Street, Albany, California 94710, United States.
authors.forename: Tara H
authors.initials: TH
authors.lastname: McHugh
mesh_terms: D000293:Adolescent; D020816:Amino Acid Motifs; D031325:Carya / Q000737:chemistry / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000276:immunology*; D012639:Seeds / Q000737:chemistry / Q000276:immunology; D055815:Young Adult
chemical_list: D055314:Seed Storage Proteins; D007073:Immunoglobulin E; C015575:vicilin protein, plant
keywords: Carya illinoinensis (Wangenh.) K. Koch; Western blot; X-ray crystallography; allergy