title: Detection of a Novel Ehrlichia Species in Haemaphysalis longicornis Tick from China.
abstract: We collected 2460 Haemaphysalis longicornis ticks from vegetation in Jiaonan County, Shandong Province, in June of 2013 and Daishan County, Zhejiang Province, China, in May of 2015. The tick DNA was subsequently amplified with nested polymerase chain reaction using Ehrlichia common 16S rRNA gene primers and Ehrlichia ewingii species-specific groEL and gltA primers. We found 0.4% (3/780) of the ticks from Zhejiang Province contained Ehrlichia DNA that was different from all known Ehrlichia species, but most closely related to E. ewingii. We concluded that a novel Ehrlichia species exists in H. longicornis ticks in China.
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Limei
authors.initials: L
authors.lastname: Luo
authors.affiliation: 2 Zhejiang Province Center for Disease Control and Prevention , Hangzhou, Zhejiang, China .
authors.forename: Jimin
authors.initials: J
authors.lastname: Sun
authors.affiliation: 3 Zhoushan City Center for Disease Control and Prevention , Zhoushan, Zhejiang, China .
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yan
authors.affiliation: 4 Daishan County Center for Disease Control and Prevention , Daishan, Zhejiang, China .
authors.forename: Chengwei
authors.initials: C
authors.lastname: Wang
authors.affiliation: 5 Huangdao District Center for Disease Control and Prevention , Qingdao, Shandong, China .
authors.forename: Zhentang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Huiju
authors.initials: H
authors.lastname: Han
authors.affiliation: 3 Zhoushan City Center for Disease Control and Prevention , Zhoushan, Zhejiang, China .
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Tong
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Liu
authors.affiliation: 2 Zhejiang Province Center for Disease Control and Prevention , Hangzhou, Zhejiang, China .
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Hongling
authors.initials: H
authors.lastname: Wen
authors.affiliation: 2 Zhejiang Province Center for Disease Control and Prevention , Hangzhou, Zhejiang, China .
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: 5 Huangdao District Center for Disease Control and Prevention , Qingdao, Shandong, China .
authors.forename: Zaifeng
authors.initials: Z
authors.lastname: Xue
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Xifeng
authors.initials: X
authors.lastname: Sun
authors.affiliation: 3 Zhoushan City Center for Disease Control and Prevention , Zhoushan, Zhejiang, China .
authors.forename: Kefeng
authors.initials: K
authors.lastname: Li
authors.affiliation: 5 Huangdao District Center for Disease Control and Prevention , Qingdao, Shandong, China .
authors.forename: Dongqiang
authors.initials: D
authors.lastname: Ma
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Jianwei
authors.initials: J
authors.lastname: Liu
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Yuting
authors.initials: Y
authors.lastname: Huang
authors.affiliation: 4 Daishan County Center for Disease Control and Prevention , Daishan, Zhejiang, China .
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .
authors.forename: Wenqian
authors.initials: W
authors.lastname: Li
authors.affiliation: 2 Zhejiang Province Center for Disease Control and Prevention , Hangzhou, Zhejiang, China .
authors.forename: Jianmin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: 1 School of Public Health, Shandong University , Jinan, Shandong, China .|6 Department of Pathology, University of Texas Medical Branch , Galveston, Texas.
authors.forename: Xue-Jie
authors.initials: XJ
authors.lastname: Yu
mesh_terms: D000818:Animals; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004536:Ehrlichia / Q000145:classification* / Q000302:isolation & purification*; D026863:Ixodidae / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial
keywords: China; Ehrlichia; Haemaphysalis longicornis; Tick-borne diseases