title: Functional networks of aging markers in the glomeruli of IgA nephropathy: a new therapeutic opportunity.
abstract: IgA nephropathy(IgAN) is the most common primary glomerular disease in China. Primary infections always occur before IgAN. However, the pathology of IgAN is still unclear. Previously we found that LL37, a protein secreted by senescent cells, was specific for the progression of IgAN, and also played a role in the neutrophil function. So we hypothesized that the infiltration of neutrophils, inflammation factors, and aging markers , which were modulated by functional networks, induced the immune response and renal injury. RNA-Sequencing (RNA-seq) can be used to study the whole transcriptome and detect splicing variants that are expressed in a specific cell type or tissue. We separate glomerulus from the renal biopsy tissues. After RNA extraction, the sequences were analyzed with Illumina HiSeq 2000/2500. 381 genes with differential expression between the IgAN patients and the healthy controls were identified. Only PLAU, JUN, and FOS were related to DNA damage, telomere dysfunction-induced aging markers, neutrophil function and IgA nephropathy. The networks showed the possibility of these genes being connected. We conclude that DNA damage and telomere dysfunction could play important roles in IgA nephropathy. In addition, neutrophils are also important factors in this disease. The networks of these markers showed the mechanism pathways that are involved in the duration of the occurrence and progression of IgA nephropathy and might be a new therapeutic opportunity for disease treatment.
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Ludan
authors.initials: L
authors.lastname: Liang
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.|School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qin
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Bingjue
authors.initials: B
authors.lastname: Li
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Lin
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Shi
authors.initials: S
authors.lastname: Feng
authors.affiliation: School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
authors.forename: Shun H
authors.initials: SH
authors.lastname: Yip
authors.affiliation: School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Physiology, Zhejiang University School of Medicine, Hangzhou, P.R. China.
authors.forename: En Yin
authors.initials: EY
authors.lastname: Lai
authors.affiliation: Centre for Genomic Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.|School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, P.R. China.|Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration Of Traditional Chinese Medicine Of China, Hangzhou, P.R. China.|Key Laboratory Of Multiple Organ Transplantation, Ministry Of Health, Key Laboratory Of Nephropathy, Zhejiang, P.R. China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D000328:Adult; D000375:Aging; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D005922:Glomerulonephritis, IGA / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D059467:Transcriptome
chemical_list: D015415:Biomarkers
keywords: IgAN; Pathology Section; RNA sequence; aging; glomerular; networks