title: Self-Powered Electrical Stimulation for Enhancing Neural Differentiation of Mesenchymal Stem Cells on Graphene-Poly(3,4-ethylenedioxythiophene) Hybrid Microfibers.
abstract: Engineered conductive scaffolds toward neural regeneration should have the ability to regulate mesenchymal stems cell (MSC) differentiation into neural lineage through an electrical stimulation-assisted culture process. In this work, a self-powered electrical stimulation-assisted neural differentiation system for MSCs was realized by combining a high effective triboelectric nanogenerator (TENG) to supply pulsed electric simulation signals and a poly(3,4-ethylenedioxythiophene) (PEDOT)-reduced graphene oxide (rGO) hybrid microfiber (80 μm in diameter) as a scaffold. The conductive PEDOT endows the rGO-PEDOT hybrid microfiber with an enhanced electrical conductivity and maintains a good cytocompatibility. MSCs cultured on this highly conductive rGO-PEDOT hybrid microfiber possess enhanced proliferation ability and good neural differentiation tendency. Importantly, by inducing electric pulses generated by the TENG as the electrical stimulation signal, which are triggered by human walking steps, neural differentiation of MSCs is dramatically improved. This study illustrates the customizability of the rGO-PEDOT hybrid microfiber for neural tissue engineering scaffolding applications, underlines the potential of a self-powered TENG electrical stimulation system for accelerating MSC differentiation into neural cells without bio/chemical cues, and suggests the TENG's practical use as a wearable stimulation system to assist nerve regeneration for a walking person.
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST) , Beijing 100083, People's Republic of China.|University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Guo
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST) , Beijing 100083, People's Republic of China.|University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST) , Beijing 100083, People's Republic of China.|University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST) , Beijing 100083, People's Republic of China.|University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University , Jinan 250100, People's Republic of China.
authors.forename: Jichuan
authors.initials: J
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University , Jinan 250100, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST) , Beijing 100083, People's Republic of China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST) , Beijing 100083, People's Republic of China.|State Key Laboratory of Crystal Materials, Shandong University , Jinan 250100, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST) , Beijing 100083, People's Republic of China.|School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332-0245, United States.
authors.forename: Zhong Lin
authors.initials: ZL
authors.lastname: Wang
mesh_terms: D019086:Bridged Bicyclo Compounds, Heterocyclic; D002454:Cell Differentiation; D004558:Electric Stimulation; D006108:Graphite; D006801:Humans; D059630:Mesenchymal Stem Cells; D053758:Nanoparticles; D011108:Polymers
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D006108:Graphite
keywords: MSCs; electrical stimulation; neural differentiation; rGO−PEDOT microfiber; self-powered TENG