title: Stereochemical Study of Sphingosine by Vibrational Circular Dichroism.
abstract: Vibrational circular dichroism (VCD) was first applied to the stereochemical analysis of sphingosine. VCD patterns derived from the C═C stretch as well as other mid-infrared (IR) regions were practical markers to discriminate all the stereoisomers of intact sphingosine. Glutaraldehyde was found as an excellent derivatizing reagent for sphingosine which improves its solubility in VCD-friendly nonpolar solvents such as chloroform and enhances the VCD intensities by forming a rigid cyclized structure.
authors.affiliation: Faculty of Advanced Life Science, Frontier Research Center for the Post-Genome Science and Technology, Hokkaido University , Kita 21, Nishi 11, Sapporo 001-0021, Japan.
authors.forename: Atsufumi
authors.initials: A
authors.lastname: Nakahashi
authors.affiliation: Faculty of Advanced Life Science, Frontier Research Center for the Post-Genome Science and Technology, Hokkaido University , Kita 21, Nishi 11, Sapporo 001-0021, Japan.
authors.forename: Ananda Kumar
authors.initials: AK
authors.lastname: C Siddegowda
authors.affiliation: Faculty of Advanced Life Science, Frontier Research Center for the Post-Genome Science and Technology, Hokkaido University , Kita 21, Nishi 11, Sapporo 001-0021, Japan.
authors.forename: Mostafa A S
authors.initials: MA
authors.lastname: Hammam
authors.affiliation: Faculty of Advanced Life Science, Frontier Research Center for the Post-Genome Science and Technology, Hokkaido University , Kita 21, Nishi 11, Sapporo 001-0021, Japan.
authors.forename: Siddabasave Gowda B
authors.initials: SG
authors.lastname: Gowda
authors.affiliation: Faculty of Advanced Life Science, Frontier Research Center for the Post-Genome Science and Technology, Hokkaido University , Kita 21, Nishi 11, Sapporo 001-0021, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Murai
authors.affiliation: Faculty of Advanced Life Science, Frontier Research Center for the Post-Genome Science and Technology, Hokkaido University , Kita 21, Nishi 11, Sapporo 001-0021, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Monde