title: A comparison of three serological tests for the diagnosis of B. owis infection in rams.
abstract: The complement fixation test (CFT), the enzyme labelled immunosorbent assay (ELISA) and the gel diffusion precipitin test (GD) were compared, for the diagnosis of Brucellu ovis infection in rams. The sensitivities of the tests in 109 rams which were shedding B. ovis in their semen were: CFT 96.3%; ELISA 97.2%; GD 91.7%. The specificities of the tests in 141 rams from non-infected flocks were: CFI 99.3%; ELISA 98.6%; GD 100%. Predictive values of the three tests were measured in 285 rams from infected flocks. Thirty-eight percent of these rams were shedding B. ovis in their semen. Predictive values of positive tests were: CFT 75.5%; ELISA 66.7%; GD 72.5%. Predictive values of negative tests were: CFI 97.1%; ELISA 97.6%; GD 93.8%.
authors.affiliation: a Animal Health Refeence Laboratory , Wallaceville Animal Research Centre , Private Bag, Upper Hutt.
authors.forename: R W
authors.initials: RW
authors.lastname: Worthington
authors.affiliation: a Animal Health Refeence Laboratory , Wallaceville Animal Research Centre , Private Bag, Upper Hutt.
authors.forename: W
authors.initials: W
authors.lastname: Weddell
authors.affiliation: a Animal Health Refeence Laboratory , Wallaceville Animal Research Centre , Private Bag, Upper Hutt.
authors.forename: M E
authors.initials: ME
authors.lastname: Penrose