title: Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster.
abstract: Perilipins are evolutionarily conserved from Drosophila to humans, the lipid storage droplet 1 (Lsd1) is a Drosophila homolog of human perilipin 1. The function of Lsd1 as a regulator of lipolysis in Drosophila has been demonstrated, as the Lsd1 mutant causes an increase of lipid droplet size. However, the functions of this gene during development are still under investigation. In order to determine the function of Lsd1 during development, Lsd1 was knocked down in Drosophila using the GAL4-UAS system. Selective knockdown of Lsd1 in the dorsal wing disc caused an atrophied wing phenotype. The generation of reactive oxygen species in the wing pouch compartment of the Lsd1-knockdown flies was significantly higher than in the control. Immunostaining with caspase-3 antibody revealed a greater number of apoptotic cells in Lsd1-knockdown wing discs than in the control. Cell death by autophagy was also induced in the knockdown flies. Moreover, cells deprived of Lsd1 showed mitochondrial expansion and decreased ATP levels. These results strongly suggest that knockdown of Lsd1 induces mitochondrial stress and the production of reactive oxygen species that result in cell death, via apoptosis and the autophagy pathway. These results highlight the roles of Drosophila Lsd1 during wing development.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, Japan. ttmen@ctu.edu.vn.
authors.forename: Tran Thanh
authors.initials: TT
authors.lastname: Men
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, Japan. tdbinh22@gmail.com.
authors.forename: Tran Duy
authors.initials: TD
authors.lastname: Binh
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto 606-8585, Japan. myamaguc@kit.ac.jp.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Yamaguchi
authors.affiliation: Department of Clinical Product Development, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, Japan. tienhuy@nagasaki-u.ac.jp.
authors.forename: Nguyen Tien
authors.initials: NT
authors.lastname: Huy
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, Japan. kame@kit.ac.jp.
authors.forename: Kaeko
authors.initials: K
authors.lastname: Kamei
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D001343:Autophagy; D053148:Caspase 3 / Q000378:metabolism; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000378:metabolism; D007150:Immunohistochemistry; D008055:Lipids / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D008055:Lipids; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D000255:Adenosine Triphosphate; C524804:Lsd-1 protein, Drosophila; D010089:Oxidoreductases, N-Demethylating; D053148:Caspase 3
keywords: Drosophila; lipid storage droplet 1; mitochondrial stress; perilipin; wing development