title: Chilling-responsive mechanisms in halophyte Puccinellia tenuiflora seedlings revealed from proteomics analysis.
abstract: Alkali grass (Puccinellia tenuiflora), a monocotyledonous perennial halophyte species, is a good pasture with great nutritional value for livestocks. It can thrive under low temperature in the saline-alkali soil of Songnen plain in northeastern China. In the present study, the chilling-responsive mechanism in P. tenuiflora leaves was investigated using physiological and proteomic approaches. After treatment of 10°C for 10 and 20days, photosynthesis, biomass, contents of osmolytes and antioxidants, and activities of reactive oxygen species scavenging enzymes were analyzed in leaves of 20-day-old seedlings. Besides, 89 chilling-responsive proteins were revealed from proteomic analysis. All the results highlighted that the growth of seedlings was inhibited due to chilling-decreased enzymes in photosynthesis, carbohydrate metabolism, and energy supplying. The accumulation of osmolytes (i.e., proline, soluble sugar, and glycine betaine) and enhancement of ascorbate-glutathione cycle and glutathione peroxidase/glutathione S-transferase pathway in leaves could minimize oxidative damage of membrane and other molecules under the chilling conditions. In addition, protein synthesis and turnover in cytoplasm and chloroplast were altered to cope with the chilling stress. This study provides valuable information for understanding the chilling-responsive and cross-tolerant mechanisms in monocotyledonous halophyte plant species.
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Meng
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
authors.forename: Yudan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Yu
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
authors.forename: Zepeng
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China. Electronic address: daishaojun@hotmail.com.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dai
mesh_terms: D000222:Adaptation, Physiological; D050260:Carbohydrate Metabolism; D002681:China; D003080:Cold Temperature; D004734:Energy Metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology; D010940:Plant Proteins / Q000032:analysis; D006109:Poaceae / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D055051:Salt-Tolerant Plants / Q000737:chemistry / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010940:Plant Proteins
keywords: Chilling; Cross-tolerance; Leaves; Proteomics; Puccinellia tenuiflora