title: Theranostic tumor targeted nanoparticles combining drug delivery with dual near infrared and19F magnetic resonance imaging modalities.
abstract: Combining imaging and drug delivery of "theranostic" nanoparticles has enabled concurrent diagnosis and therapy of diseases. Here, we describe a novel theranostic system that combines two imaging tracers, perfluorooctyl bromide (PFOB) for19F magnetic resonance imaging (MRI) and indocyanine green (ICG) for near infrared (NIR) imaging, with the chemotherapeutic agent doxorubicin (Dox) into poly (lactic-co-glycolic acid)- poly (ethylene-glycol)-folate (PLGA-PEG-folate) nanoparticles. Cell culture studies using flow cytometry, confocal laser scanning microscope imaging, and19F MRI showed enhanced uptake of nanoparticles via folate receptors expressed on human nasopharyngeal epidermal carcinoma (KB) cells. In vivo, higher MRI and fluorescence signals were obtained from tumors with19F MRI and NIR, respectively, using folate-receptor-targeted nanoparticles compared with non-targeted equivalents. An in vitro cytotoxicity assay showed that folate-targeted nanoparticles were able to kill cancer cells more efficiently than non-folate conjugated particles. Our results suggest a potential use of PLGA-PEG-folate PFOB/ICG/Dox nanoparticles as a targeted chemotherapy agent traceable by either19F MRI or NIR imaging.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark; LUNA Nanomedicine Center, Aarhus University, Denmark.
authors.forename: Hieu
authors.initials: H
authors.lastname: Vu-Quang
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark; Center for Insoluble Protein Structures (inSPIN), Aarhus University, Denmark.
authors.forename: Mads Sloth
authors.initials: MS
authors.lastname: Vinding
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark; Center for Insoluble Protein Structures (inSPIN), Aarhus University, Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Nielsen
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark; Center for Insoluble Protein Structures (inSPIN), Aarhus University, Denmark.
authors.forename: Marcus Görge
authors.initials: MG
authors.lastname: Ullisch
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark; LUNA Nanomedicine Center, Aarhus University, Denmark; Center for Insoluble Protein Structures (inSPIN), Aarhus University, Denmark; Department of Chemistry, Aarhus University, Denmark.
authors.forename: Niels Chr
authors.initials: NC
authors.lastname: Nielsen
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Denmark; LUNA Nanomedicine Center, Aarhus University, Denmark; Center for Insoluble Protein Structures (inSPIN), Aarhus University, Denmark; Department of Molecular Biology, Aarhus University, Denmark. Electronic address: kjems@inano.au.dk.
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D004317:Doxorubicin / Q000008:administration & dosage*; D006801:Humans; D008279:Magnetic Resonance Imaging; D053758:Nanoparticles; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy; D000068936:Theranostic Nanomedicine
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin
keywords: (19)F MRI; Doxorubicin; Folate; ICG; PFOB; PLGA