title: [Determination of Triptolide and Wilforlide A in Biological Samples by LC-MS/MS].
abstract: OBJECTIVE
  To determinate triptolide and wilforlide A in biological samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and to verify the method.
  
  
  METHODS
  After 0.4 mL blood, urine or 0.4 g hepatic tissues with internal standard were extracted by ethyl acetate, they were separated on a Allure PFP Propyl (100 mm x 2.1 mm, 5 µm) with a mobile phase of methanol-20 mmol/L ammonium acetate using gradient elution. For mass spectrometric detection, electrospray ionization (ESI⁺) in positive mode was elected and the data was collected using multiple-reaction monitoring (MRM).
  
  
  RESULTS
  The linearity was good (r > 0.995 0) and the limit of detection was 2 ng/mL or 2 ng/g for triptolide and wilforlide A. The recovery was 61.08%-102.98%. The intra-day and inter-day precision was less than 12.58% for each biological sample, and the accuracy was 90.61%-105.80%.
  
  
  CONCLUSIONS
  This method is simple, convenient and good selective, and could be applied to analysis of triptolide and wilforlide A in different biological samples. And the method may provide technical support for forensic medicine identification, clinical diagnosis and treatment of tripterygium wilfordii Hook. f. poisoning.
authors.forename: Jin-xiao
authors.initials: JX
authors.lastname: Zhai
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000097:blood* / Q000652:urine*; D004852:Epoxy Compounds / Q000097:blood / Q000652:urine; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D010616:Phenanthrenes / Q000097:blood* / Q000652:urine*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide; D009828:Oleanolic Acid; C052981:wilforlide A