title: Expression analysis of Baf60c during heart regeneration in axolotls and neonatal mice.
abstract: Some organisms, such as zebrafish, urodele amphibians, and newborn mice, have a capacity for heart regeneration following injury. However, adult mammals fail to regenerate their hearts. To know why newborn mice can regenerate their hearts, we focused on epigenetic factors, which are involved in cell differentiation in many tissues. Baf60c (BRG1/BRM-associated factor 60c), a component of ATP-dependent chromatin-remodeling complexes, has an essential role for cardiomyocyte differentiation at the early heart development. To address the function of Baf60c in postnatal heart homeostasis and regeneration, we examined the detailed expression/localization patterns of Baf60c in both mice and axolotls. In the mouse heart development, Baf60c was highly expressed in the entire heart at the early stages, but gradually downregulated at the postnatal stages. During heart regeneration in neonatal mice and axolotls, Baf60c expression was strongly upregulated after resection. Interestingly, the timing of Baf60c upregulation after resection was consistent with the temporal dynamics of cardiomyocyte proliferation. Moreover, knockdown of Baf60c downregulated proliferation of neonatal mouse cardiomyocytes. These data suggested that Baf60c plays an important role in cardiomyocyte proliferation in heart development and regeneration. This is the first study indicating that Baf60c contributes to the heart regeneration in vertebrates.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-0033, Japan.|Division of Cardiovascular Regeneration, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-0032, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakamura
authors.affiliation: Division of Cardiovascular Regeneration, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-0032, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Koshiba-Takeuchi
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, 565-0871, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Tsuchiya
authors.affiliation: Division of Cardiovascular Regeneration, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-0032, Japan.
authors.forename: Mizuyo
authors.initials: M
authors.lastname: Kojima
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-0033, Japan.|Division of Cardiovascular Regeneration, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-0032, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Miyazawa
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-0033, Japan.|Division of Cardiovascular Regeneration, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-0032, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Ito
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, 565-0871, Japan.|Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, 651-2492, Japan.
authors.forename: Hidesato
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-0033, Japan.|Division of Cardiovascular Regeneration, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-0032, Japan.
authors.forename: Jun K
authors.initials: JK
authors.lastname: Takeuchi
mesh_terms: D000558:Ambystoma mexicanum; D029845:Amphibian Proteins / Q000096:biosynthesis*; D000818:Animals; D049109:Cell Proliferation / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D006321:Heart / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009124:Muscle Proteins / Q000096:biosynthesis*; D032383:Myocytes, Cardiac / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*
chemical_list: D029845:Amphibian Proteins; D002868:Chromosomal Proteins, Non-Histone; D009124:Muscle Proteins; C494055:Smarcd3 protein, mouse
keywords: Baf60c; axolotl; heart; mouse; regeneration