title: Electrochemiluminescence at Bare and DNA-Coated Graphite Electrodes in 3D-Printed Fluidic Devices.
abstract: Clear plastic fluidic devices with ports for incorporating electrodes to enable electrochemiluminescence (ECL) measurements were prepared using a low-cost, desktop three-dimensional (3D) printer based on stereolithography. Electrodes consisted of 0.5 mm pencil graphite rods and 0.5 mm silver wires inserted into commercially available 1/4 in.-28 threaded fittings. A bioimaging system equipped with a CCD camera was used to measure ECL generated at electrodes and small arrays using 0.2 M phosphate buffer solutions containing tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate ([Ru(bpy)3]2+) with 100 mM tri-n-propylamine (TPA) as the coreactant. ECL signals produced at pencil graphite working electrodes were linear with respect to [Ru(bpy)3]2+concentration for 9-900 μM [Ru(bpy)3]2+. The detection limit was found to be 7 μM using the CCD camera with exposure time set at 10 s. Electrode-to-electrode ECL signals varied by ±7.5%. Device performance was further evaluated using pencil graphite electrodes coated with multilayer poly(diallyldimethylammonium chloride) (PDDA)/DNA films. In these experiments, ECL resulted from the reaction of [Ru(bpy)3]3+with guanines of DNA. ECL produced at these thin-film electrodes was linear with respect to [Ru(bpy)3]2+concentration from 180 to 800 μM. These studies provide the first demonstration of ECL measurements obtained using a 3D-printed closed-channel fluidic device platform. The affordable, high-resolution 3D printer used in these studies enables easy, fast, and adaptable prototyping of fluidic devices capable of incorporating electrodes for measuring ECL.
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, United States.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Bishop
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, United States.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Satterwhite-Warden
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, United States.
authors.forename: Itti
authors.initials: I
authors.lastname: Bist
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Chen
authors.affiliation: Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, United States; Institute of Materials Science, University of Connecticut, Storrs, Connecticut 06269-3136, United States; Department of Surgery and Neag Cancer Center, University of Connecticut Health Center, Farmington, Connecticut 06030, United States; School of Chemistry, National University of Ireland at Galway, Galway, Ireland.
authors.forename: James F
authors.initials: JF
authors.lastname: Rusling
keywords: 3D-printed fluidics; DNA oxidation; biosensing; electrochemiluminescence; stereolithography