title: [Downregulation of proteinase activated receptor 4 inhibits migration of SW620 human colorectal cancer cells].
abstract: OBJECTIVE
  To establish the human colorectal cancer cell model SW620/PAR4D with inducible suppression of proteinase activated receptor 4 (PAR4) expression, and investigate the role PAR4 plays in the proliferation and migration of cancer cells.
  
  
  METHODS
  A human colorectal cancer cell line with tetracycline-inducible expression regulatory system, namely SW620/Tet-on, was established; inducible expression lentiviral vector with artificial microRNA targeting PAR4, pLVX-Tight-Puro-PAR4-miR, was constructed and transfected into SW620/Tet-on to make an inducible PAR4-suppressed cell model SW620/PAR4D. Western blotting was used to confirm the suppression of PAR4 expression after the doxycycline (DOX) treatment. CCK-8 assay was used to evaluate the impact of suppressed PAR4 expression on cell proliferation, and wound-healing assay was used to analyze the migration of the cells.
  
  
  RESULTS
  The SW620/PAR4D cell model was established successfully. Suppression of PAR4 expression by DOX treatment had no significant impact on the growth/proliferation of SW620/PAR4D cells, but markedly inhibited the cell migration.
  
  
  CONCLUSIONS
  Suppression of PAR4 expression has no significant effect on the proliferation of SW620 cells, but can inhibit the migration of the cells.
authors.affiliation: Clinical Research Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Clinical Research Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Chunchun
authors.initials: C
authors.lastname: Li
authors.affiliation: Clinical Research Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Clinical Research Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009; Chu Kochen Honors College, Zhejiang University, Hangzhou 310058, China. *Corresponding authors, E-mail: caoj@zju.edu.cn.
authors.forename: Jingjia
authors.initials: J
authors.lastname: Ye
authors.affiliation: Clinical Research Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. *Corresponding authors, E-mail: yej001@zju.edu.cn.
authors.forename: Jiang
authors.initials: J
authors.lastname: Cao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D015536:Down-Regulation; D004318:Doxycycline / Q000494:pharmacology; D006801:Humans; D010957:Plasmids; D018179:Receptors, Thrombin / Q000235:genetics / Q000502:physiology*
chemical_list: D018179:Receptors, Thrombin; C112908:protease-activated receptor 4; D004318:Doxycycline