title: MicroRNA-127-5p regulates osteopontin expression and osteopontin-mediated proliferation of human chondrocytes.
abstract: The aim of this study was to determine the specific microRNA (miRNA) that regulates expression of osteopontin (OPN) in osteoarthritis (OA). The potential regulatory miRNAs for OPN messenger RNA (mRNA) were predicted by miRNA prediction programs. Among eight potential regulatory miRNAs, miR-220b, miR-513a-3p and miR-548n increased, while miR-181a, miR-181b, miR-181c, miR-181d and miR-127-5p decreased in OA patients. miRNA-127-5p mimics suppressed OPN production as well as the activity of a reporter construct containing the 3'-UTR of human OPN mRNA. In addition, mutation of miR-127-5p binding site in the 3'-UTR of OPN mRNA abolished miR-127-5p-mediated repression of reporter activity. Conversely, treatment with miR-127-5p inhibitor increased reporter activity and OPN production. Interestingly, miR-127-5p inhibited proliferation of chondrocytes through OPN. In conclusion, miRNA-127-5p is an important regulator of OPN in human chondrocytes and may contribute to the development of OA.
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.|Department of Orthopaedics, Second People's Hospital of Jingmen, Jingmen, 448000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tu
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Zhenhan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Bone and Joint, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzheng, 518020, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.|Department of Joint Surgery, Changsha Central Hospital, Changsha, 410000, China.
authors.forename: Liangjun
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Lei
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism; D053495:Osteopontin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C547276:MIRN127 microRNA, human; D035683:MicroRNAs; C506895:SPP1 protein, human; D053495:Osteopontin