title: Paper-based archiving of biological samples from fish for detecting betanodavirus.
abstract: This study was carried out to evaluate the efficiency of the Flinders Technology Associates (FTA(®)) card (Whatman(®)) as a sampling device and storage platform for RNA from betanodavirus-infected biological samples (viz., larvae, broodstock, cell culture supernatants and rearing seawater spiked with infected materials). The study showed that FTA cards can be used to detect betanodaviruses by reverse transcription-polymerase chain reaction (RT-PCR). The diagnostic efficiency of RT-PCR from all sample types on FTA cards decreased after 21 days of storage at 4 °C, although the virus could be detected up to 28 days by nested RT-PCR. The FTA card protocol thus provides a supplementary method for quick and easy collection of samples, preservation of RNA on a dry storage basis, and detection of betanodavirus-infected fish.
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, R.A Puram, Chennai, 600 028, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Navaneeth Krishnan
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, R.A Puram, Chennai, 600 028, Tamil Nadu, India.
authors.forename: T
authors.initials: T
authors.lastname: Bhuvaneswari
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, R.A Puram, Chennai, 600 028, Tamil Nadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Ezhil Praveena
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, R.A Puram, Chennai, 600 028, Tamil Nadu, India. kpjithendran@yahoo.com.
authors.forename: K P
authors.initials: KP
authors.lastname: Jithendran
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000175:diagnosis / Q000821:virology*; D005399:Fishes; D030381:Nodaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010209:Paper; D010802:Phylogeny; D012327:RNA Virus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*
chemical_list: D012367:RNA, Viral