title: [Chemical constituents from endophyte Chaetomium globosum in Imperata cylindrical].
abstract: Isolation and purification of chemical constituents from solid culture of endophyte Chaetomium globosum in Imperata cylindrical was performed through silica gel column chromatography, gel filtration over Sephadex LH-20 and preparative HPLC. Nine compounds were obtained and their structures were determined as chaetoglobosin F(1), chaetoglobosin Fex(2), chaetoglobosin E(3) cytoglobosin A(4), penochalasin C(S), isochaetoglobosin D (6), N-benzoylphenylalaninyl-N-benzoyphenylalaninate(7), uracil(8) and 5-methyluracil(9), respectively, based on HR-MS and NMR data and comparison with literatures. Compound 7 was isolated from Chaeeomium sp. for the first time. In vitro cytotoxicity of compounds was evaluated using MTT mothed and 1,3,4 and 5 showed inhibition activity to the human cervical carcinoma cell HeLa with IC50 values of 99.43, 23.77, 97.92, 86.25 micromol x L(-1), while positive cotolocisnin Ad apno1ch alse IC50 24.33 micromol x L(-1).
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhong-qi
authors.initials: ZQ
authors.lastname: Wei
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Yong-chun
authors.initials: YC
authors.lastname: Song
mesh_terms: D001685:Biological Factors / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002597:Chaetomium / Q000737:chemistry*; D060026:Endophytes / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D006109:Poaceae / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001685:Biological Factors