title: Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust.
abstract: Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases and can cause severe yield losses in many regions of the world. Because of the large size and complexity of wheat genome, it is difficult to study the molecular mechanism of interaction between wheat and PST. Brachypodium distachyon has become a model system for temperate grasses' functional genomics research. The phenotypic evaluation showed that the response of Brachypodium distachyon to PST was nonhost resistance (NHR), which allowed us to present this plant-pathogen system as a model to explore the immune response and the molecular mechanism underlying wheat and PST. Here we reported the generation of about 7,000 T-DNA insertion lines based on a highly efficient Agrobacterium-mediated transformation system. Hundreds of mutants either more susceptible or more resistant to PST than that of the wild type Bd21 were obtained. The three putative target genes, Bradi5g17540, BdMYB102 and Bradi5g11590, of three T-DNA insertion mutants could be involved in NHR of Brachypodium distachyon to wheat stripe rust. The systemic pathologic study of this T-DNA mutants would broaden our knowledge of NHR, and assist in breeding wheat cultivars with durable resistance.
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Tianyue
authors.initials: T
authors.lastname: An
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|Institute of Plant Sciences, University of Sindh, Jamshoro, 76080, Pakistan.
authors.forename: Hadi
authors.initials: H
authors.lastname: Bux
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Qi
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D001487:Basidiomycota / Q000235:genetics / Q000472:pathogenicity; D058431:Brachypodium / Q000235:genetics* / Q000382:microbiology; D001947:Breeding; D004269:DNA, Bacterial / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA