title: Protein crystal screening and characterization for serial femtosecond nanocrystallography.
abstract: The recent development of X-ray free electron lasers (XFELs) has spurred the development of serial femtosecond nanocrystallography (SFX) which, for the first time, is enabling structure retrieval from sub-micron protein crystals. Although there are already a growing number of structures published using SFX, the technology is still very new and presents a number of unique challenges as well as opportunities for structural biologists. One of the biggest barriers to the success of SFX experiments is the preparation and selection of suitable protein crystal samples. Here we outline a protocol for preparing and screening for suitable XFEL targets.
authors.affiliation: ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
authors.forename: Connie
authors.initials: C
authors.lastname: Darmanin
authors.affiliation: ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
authors.forename: Jamie
authors.initials: J
authors.lastname: Strachan
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Adda
authors.affiliation: School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, 4072, Australia.|Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, QLD, 4222, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ve
authors.affiliation: School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, 4072, Australia.
authors.forename: Bostjan
authors.initials: B
authors.lastname: Kobe
authors.affiliation: ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.|Melbourne Centre for Nanofabrication, Melbourne, 3168, Australia.
authors.forename: Brian
authors.initials: B
authors.lastname: Abbey