title: Evaluation of anti-metastatic potential of Cisplatin polymeric nanocarriers on B16F10 melanoma cells.
abstract: Nanoparticles are being increasingly used in the field of cancer treatment due to their unique properties and advantages. The aim of the present research work was to prepare and characterize a polymeric albumin nanosystem for Cisplatin and evaluate its in-vitro efficacy against B16F10 melanoma. The developed nanoparticles were almost spherical in shape with a particle size in the range of 150-300 nm, low polydispersity values and about 80% drug entrapment efficiency. Albumin nanocarriers sustained the release of Cisplatin for more than 48 h, suggesting the reduction in dosing schedule for this drug. The results from in-vitro cell line studies indicated the dose dependent cytotoxic potential of drug loaded albumin nanoparticles, their potential to inhibit cell proliferation and induce morphological changes. In addition, these nanoparticles exhibited superiority to Cisplatin in hampering the cell migration. Developed nanoparticles caused cell cycle arrest along with time and concentration dependent cellular uptake in B16F10 cell line. These results signify that the prepared Cisplatin albumin nanoparticles could serve as a promising approach for B16F10 melanoma treatment.
authors.affiliation: C.U. Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz (W), Mumbai 400049, India.
authors.forename: Shruti S
authors.initials: SS
authors.lastname: Shrikhande
authors.affiliation: C.U. Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz (W), Mumbai 400049, India.
authors.forename: Darshana S
authors.initials: DS
authors.lastname: Jain
authors.affiliation: C.U. Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz (W), Mumbai 400049, India.
authors.forename: Rajani B
authors.initials: RB
authors.lastname: Athawale
authors.affiliation: SVKM's Dr. Bhanuben Nanavati College of Pharmacy, V.M. Road, Vile Parle (W), Mumbai 400056, India.
authors.forename: Amrita N
authors.initials: AN
authors.lastname: Bajaj
authors.affiliation: Gude Lab, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.
authors.forename: Peeyush
authors.initials: P
authors.lastname: Goel
authors.affiliation: Gude Lab, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.
authors.forename: Zahid
authors.initials: Z
authors.lastname: Kamran
authors.affiliation: Gude Lab, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.
authors.forename: Yuvraj
authors.initials: Y
authors.lastname: Nikam
authors.affiliation: Gude Lab, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.
authors.forename: Rajiv
authors.initials: R
authors.lastname: Gude
keywords: B16F10 melanoma; Cisplatin; Human serum albumin; Nanoparticles