title: Production and identification of iturin A lipopeptide from Bacillus methyltrophicus TEB1 for control of Phoma tracheiphila.
abstract: A lipopeptide-producing endophytic Bacillus methyltrophicus TEB1 strain exhibited potent antifungal activity against Phoma tracheiphila. Lipopeptide production started at the early growth phase plateaued after 36 h of culture where it reduced the mycelium growth by 80%. The crude lipopeptide extract harvested at the stationary phase efficiently inhibited the growth of P. tracheiphila mycelium and MIC values displaying 50 and 90% inhibition of conidia germination were around 47.5 and 100 μg ml(-1) , respectively. Increasing lipopeptide extract till 3 mg ml(-1) induced 10% swelling and 3% crumbling of P. tracheiphila conidia whereas 5 mg ml(-1) induced 40% swelling and 20% crumbling. Mass spectrometry analysis of the lipopeptide extract indicated that surfactin production took place from 12 to 20 h, iturin A from 16 to 72 h, and fengycin from 12 to 72 h and that the main active compound against P. tracheiphila was identified as C15 iturin A lipopeptide. Iturin A appeared as a potential biological control agent able to substitute the currently used chemical pesticides in agriculture.
authors.affiliation: Laboratoire des Substances Bioactives, CBBC, Hammam-Lif, Tunisia.|Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, Tunis, Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Kalai-Grami
authors.affiliation: Laboratoire des Substances Bioactives, CBBC, Hammam-Lif, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Karkouch
authors.affiliation: Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, Tunis, Tunisia.
authors.forename: Omar
authors.initials: O
authors.lastname: Naili
authors.affiliation: Laboratoire des Substances Bioactives, CBBC, Hammam-Lif, Tunisia.
authors.forename: Imen Ben
authors.initials: IB
authors.lastname: Slimene
authors.affiliation: Laboratoire des Substances Bioactives, CBBC, Hammam-Lif, Tunisia.
authors.forename: Salem
authors.initials: S
authors.lastname: Elkahoui
authors.affiliation: Laboratoire des Substances Bioactives, CBBC, Hammam-Lif, Tunisia.
authors.forename: Roudaina Ben
authors.initials: RB
authors.lastname: Zekri
authors.affiliation: Laboratoire des Substances Bioactives, CBBC, Hammam-Lif, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Touati
authors.affiliation: Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, Tunis, Tunisia.
authors.forename: Monia
authors.initials: M
authors.lastname: Mnari-Hattab
authors.affiliation: Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA, Hedi Karray, Tunis, Tunisia.
authors.forename: Mohamed Rabeh
authors.initials: MR
authors.lastname: Hajlaoui
authors.affiliation: Laboratoire des Substances Bioactives, CBBC, Hammam-Lif, Tunisia.
authors.forename: Ferid
authors.initials: F
authors.lastname: Limam
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development*; D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D061046:Biological Control Agents / Q000494:pharmacology*; D055666:Lipopeptides / Q000096:biosynthesis / Q000494:pharmacology; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development*; D010456:Peptides, Cyclic / Q000096:biosynthesis / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology*; D013172:Spores, Fungal / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D061046:Biological Control Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic; C049972:fengycin; C009365:surfactin peptide; C013579:iturin A
keywords: Bacillus methyltrophicus; Iturin A; Lipopeptides; Mal secco; Phoma tracheiphila