title: Laboratory Evaluation of a Point-of-Care Downward-Flow Assay for Simultaneous Detection of Antibodies to Treponema pallidum and Human Immunodeficiency Virus.
abstract: Combining the detection of syphilis and HIV antibodies into one point-of-care test integrates syphilis screening into already existing HIV screening programs, which may be particularly beneficial in settings such as antenatal care. Using the INSTI Multiplex downward-flow immunoassay, we tested 200 stored serum samples from high-risk patients enrolled in a longitudinal study on HIV infection and syphilis in Peruvian men who have sex with men and transgender women. This rapid assay detected HIV and Treponema pallidum serum antibodies with sensitivities of 100% (95% confidence interval [CI], 95.9% to 100%) and 87.4% (95% CI, 81.4% to 92.0%), respectively, and specificities of 95.5% (95% CI, 89.9% to 98.5%) and 97.0% (95% CI, 84.2% to 99.9%), respectively (n = 200). The sensitivity for syphilis antibody detection was higher in patients with a rapid plasma reagin titer of ≥1:8 (97.3%) than in those with a titer of ≤1:4 (90%) or a nonreactive titer (66.7%).
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of California Los Angeles, Los Angeles, California, USA sherbstdecortina@mednet.ucla.edu.
authors.forename: S
authors.initials: S
authors.lastname: Herbst de Cortina
authors.affiliation: Division of Global Public Health, Department of Medicine, University of California San Diego, La Jolla, California, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Bristow
authors.affiliation: Unit of Health, Sexuality, and Human Development, and Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Miraflores, Lima, Peru.
authors.forename: S K
authors.initials: SK
authors.lastname: Vargas
authors.affiliation: Unit of Health, Sexuality, and Human Development, and Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Miraflores, Lima, Peru.
authors.forename: D G
authors.initials: DG
authors.lastname: Perez
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of California Los Angeles, Los Angeles, California, USA.|Unit of Health, Sexuality, and Human Development, and Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Miraflores, Lima, Peru.
authors.forename: K A
authors.initials: KA
authors.lastname: Konda
authors.affiliation: Unit of Health, Sexuality, and Human Development, and Laboratory of Sexual Health, Universidad Peruana Cayetano Heredia, Miraflores, Lima, Peru.
authors.forename: C F
authors.initials: CF
authors.lastname: Caceres
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of California Los Angeles, Los Angeles, California, USA.|Department of Epidemiology, Fielding School of Public Health, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Klausner
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006678:HIV / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008137:Longitudinal Studies; D008297:Male; D010568:Peru; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000175:diagnosis*; D063106:Transgender Persons; D014210:Treponema pallidum / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D015483:HIV Antibodies