title: Initiator-catalyzed self-assembly of duplex-looped DNA hairpin motif based on strand displacement reaction for logic operations and amplified biosensing.
abstract: Here we program an initiator-catalyzed self-assembly of duplex-looped DNA hairpin motif based on strand displacement reaction. Due to the recycling of initiator and performance in a cascade manner, this system is versatilely extended to logic operations, including the construction of concatenated logic circuits with a feedback function and a biocomputing keypad-lock security system. Compared with previously reported molecular security systems, the prominent feature of our keypad lock is that it can be spontaneously reset and recycled with no need of any external stimulus and human intervention. Moreover, through integrating with an isothermal amplification technique of rolling circle amplification (RCA), this programming catalytic DNA self-assembly strategy readily achieves sensitive and selective biosensing of initiator. Importantly, a magnetic graphene oxide (MGO) is introduced to remarkably reduced background, which plays an important role in enhancing the signal-to-noise ratio and improving the detection sensitivity. Therefore, the proposed sophisticated DNA strand displacement-based methodology with engineering dynamic functions may find broad applications in the construction of programming DNA nanostructures, amplification biosensing platform, and large-scale DNA circuits.
authors.affiliation: Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, College of Chemistry and Chemical Engineering, Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao University, Qingdao 266071, PR China. Electronic address: bisai11@126.com.
authors.forename: Sai
authors.initials: S
authors.lastname: Bi
authors.affiliation: Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, College of Chemistry and Chemical Engineering, Laboratory of Fiber Materials and Modern Textiles, the Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao University, Qingdao 266071, PR China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Yue
authors.affiliation: Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory for Fishery Resources and Eco-environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Ye
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009690:Nucleic Acid Conformation; D010087:Oxides / Q000737:chemistry
chemical_list: D010087:Oxides; D006108:Graphite; D004247:DNA
keywords: Biosensor; Catalytic self-assembly; Keypad lock; Logic gates; Strand displacement