title: Self-assembly of novel fluorescent quantum dot-cerasome hybrid for bioelectrochemistry.
abstract: A novel fluorescent nanohybrid was fabricated via the self-assembly of semiconductive quantum dots (QDs) on biocompatible cerasomes. The nanohybrid (denoted as QDs-cerasome) was used as an electrode material for visible protein immobilization and bioelectrochemistry. The morphology and surface properties of the QDs-cerasome hybrid were characterized by transmission electron microscopies, atomic force microscopies and zeta potential measurements. Because the QDs-cerasome hybrid possessed a positive charge in aqueous solution, it could be used as a matrix to immobilize negatively charged hemoglobin (Hb) via electrostatic interaction. Ultraviolet-visible spectroscopy demonstrated that Hb was immobilized on the hybrid matrix without denaturation. The fluorescence of the QDs-cerasome was quenched as Hb was immobilized, indicating that the protein immobilization process could be visibly detected. Compared with protein electrodes constructed using a single-component material, including Hb-QDs/GC and Hb-cerasome/GC electrodes, the Hb-QDs-cerasome/GC electrode not only realized enhanced direct electrochemistry, but also displayed higher sensitivity and a wider linear range toward the detection of hydrogen peroxide because of the synergistic effect of the QDs and cerasomes. The experimental results demonstrate that this fluorescent multicomponent hybrid material provides a novel and effective platform to immobilize a redox protein to realize direct electrochemistry. As such, this hybrid shows promise for application in third-generation electrochemical biosensors.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China; Liaoning Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials (Liaoning University), Shenyang 110036, China.
authors.forename: Daliang
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China; Liaoning Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials (Liaoning University), Shenyang 110036, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhuang
authors.affiliation: College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China; Liaoning Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials (Liaoning University), Shenyang 110036, China.
authors.forename: Shuyao
authors.initials: S
authors.lastname: Wu
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kikuchi
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China.
authors.forename: Zhengbo
authors.initials: Z
authors.lastname: Han
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China. Electronic address: zhangq@lnu.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China; Liaoning Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials (Liaoning University), Shenyang 110036, China. Electronic address: songlab@lnu.edu.cn.
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Song
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D055664:Electrochemical Techniques; D004566:Electrodes; D006454:Hemoglobins; D006861:Hydrogen Peroxide; D045663:Quantum Dots
chemical_list: D006454:Hemoglobins; D006861:Hydrogen Peroxide
keywords: Cerasome; Direct electrochemistry; Hemoglobin; Quantum dots; Self-assembly; Visible protein immobilization