title: Wheat transcription factor TaWRKY70 is positively involved in high-temperature seedling plant resistance to Puccinia striiformis f. sp. tritici.
abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease of wheat (Triticum aestivum) worldwide. Wheat high-temperature seedling plant (HTSP) resistance to Pst is non-race-specific and durable. WRKY transcription factors have been proven to play important roles in plant defence responses to attacks by several pathogens. However, there is no direct evidence as to whether WRKY transcription factors play a role in HTSP resistance to Pst. We isolated a WRKY gene, named TaWRKY70, from wheat cultivar Xiaoyan 6. The expression level of TaWRKY70 was increased significantly when exposed to high temperatures (HTs) during the initial symptom expression stage of Pst infection. The expression of this gene increased in plants treated with ethylene (ET), salicylic acid (SA) and cold (4°C) stresses, but decreased in plants treated with methyl jasmonate (MeJA) and heat (40°C) stresses. Silencing of TaWRKY70 led to greater susceptibility to Pst (in terms of the increase in length of uredinial pustules and the decrease in the number of necrotic cells) compared with non-silenced plants when exposed to HT during the initial symptom expression stage of Pst infection, coinciding with expression changes of the ET- and SA-responsive genes TaPIE1 and TaPR1.1. In contrast, the expression level of the jasmonic acid (JA)-responsive gene TaAOS was not affected by TaWRKY70. These results indicate that TaWRKY70 is positively involved in HTSP resistance, during which SA and ET signalling are probably activated.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi, 712100, China.
authors.forename: Junjuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi, 712100, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi, 712100, China.
authors.forename: Fei
authors.initials: F
authors.lastname: An
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi, 712100, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi, 712100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: Agricultural Research Service, United States Department of Agriculture and Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.
authors.forename: Xianming
authors.initials: X
authors.lastname: Chen
authors.affiliation: East Malling Research, New Road, East Malling, Kent, ME19 6BJ, UK.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi, 712100, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Hu
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001487:Basidiomycota / Q000472:pathogenicity*; D003080:Cold Temperature; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D006358:Hot Temperature; D054883:Oxylipins / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology; D036226:Seedlings / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics / Q000378:metabolism / Q000382:microbiology
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010940:Plant Proteins; D014157:Transcription Factors; C072239:methyl jasmonate; C036216:ethylene; D020156:Salicylic Acid
keywords: Puccinia striiformis f. sp. tritici; WRKY70 transcription factor; high-temperature seedling plant resistance; virus-induced gene silencing