title: [Factor Xa Promotes Differentiation of Meg-01 Cell Line].
abstract: OBJECTIVE
  To investigate the effect and mechanism of Factor Xa on the differentiation of Meg-01 cells into platelet-like particles.
  
  
  METHODS
  The Meg-01 cells were used as experimental object, Factor Xa was used as agonist. Cell proliferation was detected by CCK-8 assay. The viability of platelet-like particles was analyzed by AlamaBlue kit. MAPK/ERK pathway and PI3K/AKT pathway were assayed by Western blot. The expression of CD41b was analyzed by Western blot and flow cytometry. Cell cycle and apoptosis were detected by flow cytometry.
  
  
  RESULTS
  The Factor Xa (1 µg/ml) inhibited cell viability, induced apoptosis. Factor Xa triggered cell arrest at the G(2)/M stage and down-regulated the expression of SKP2. After Meg-01 cells were stimulated by Factor Xa, the expression of CD41b was up-regulated and the MAPK/ERK pathway and PI3K/AKT pathway were activated. The platelets-like particles stimulated by FXa activation were viable.
  
  
  CONCLUSIONS
  The Factor Xa maybe display some effect on the differentiation of megakaryocytes into platelets.
authors.affiliation: Department of Light Industry Technique and Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong Province, China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Department of Immunology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Meng-Kai
authors.initials: MK
authors.lastname: Ge
authors.affiliation: Department of Immunology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: ytlv@sina.com.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Yu
authors.affiliation: Department of Light Industry Technique and Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, Shandong Province, China. E-mail: yuap117@163.com.
authors.forename: Ying-Tao
authors.initials: YT
authors.lastname: Lyu
mesh_terms: D017209:Apoptosis; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D059447:Cell Cycle Checkpoints; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D015951:Factor Xa / Q000494:pharmacology*; D006801:Humans; D020935:MAP Kinase Signaling System; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D015951:Factor Xa