title: Insights into the dN/dS ratio heterogeneity between brain specific genes and widely expressed genes in species of different complexity.
abstract: In mammals, it has long been suggested that brain-specific genes (BSGs) and widely expressed genes (WEGs) have seemingly lower dN/dS ratio than any other gene sets. However, to what extent these genes differ in their dN/dS ratio has still remained controversial. Here, we have revealed lower dN/dS ratio of BSGs than WEGs in human-mouse, human-orangutan, human-chimpanzee and mouse-rat orthologous pair. The significance level of dN/dS ratio difference indicates a trend of decreasing difference as complexity of compared pairs increases. Further studies with the human-mouse pair revealed that, removal of the duplicated genes from both the dataset has nullified this difference which dictates a vital role of duplicated genes in governing the selection pressure. Conclusively, higher paralog number, expression level, and longer regulatory region length of BSGs allow fewer nucleotide substitutions within them. Our results show for the first time to our knowledge lower dN/dS ratio of BSGs than WEGs.
authors.affiliation: Bioinformatics Centre, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700 054, India.
authors.forename: Kakali
authors.initials: K
authors.lastname: Biswas
authors.affiliation: Bioinformatics Centre, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700 054, India.
authors.forename: Sandip
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Bioinformatics Centre, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700 054, India. Electronic address: soumita@jcbose.ac.in.
authors.forename: Soumita
authors.initials: S
authors.lastname: Podder
authors.affiliation: Bioinformatics Centre, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700 054, India. Electronic address: tapash@jcbose.ac.in.
authors.forename: Tapash Chandra
authors.initials: TC
authors.lastname: Ghosh
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001921:Brain / Q000378:metabolism*; D030541:Databases, Genetic; D030562:Databases, Protein; D019143:Evolution, Molecular; D020440:Gene Duplication; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006801:Humans; D007438:Introns / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics; D059645:Mutation Rate; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012641:Selection, Genetic; D013045:Species Specificity
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D011506:Proteins
keywords: Brain-specific genes; Functional divergence; Intronic burden; Widely-expressed genes; dN/dS ratio