title: Mesenchymal stem cells and endothelial progenitor cells accelerate intra-aneurysmal tissue organization after treatment with SDF-1α-coated coils.
abstract: Recurrences of aneurysms remain the major drawback of detachable coils for the endovascular treatment of intracranial aneurysms. The aim of the present study is to develop new modified coils, coating the surface of platinum coils with silk fibroin (SF) consisting of stromal cell-derived factor-1α (SDF-1α), and evaluate its acceleration of organization of cavities and reduction of lumen size in a rat aneurysm model. The morphological characteristics of SDF-1α-coated coils were examined using scanning electron microscopy (SEM). Fifty experimental aneurysms were created and randomly divided into five groups: three groups were embolized with SDF-1α-coated coils (8 mm) and two of these groups need transplantation of mesenchymal stem cells (MSCs) or endothelial progenitor cells (EPCs); one group was embolized with bare coils (8 mm) and another group severed as control. After coil implantation for 14 or 28 days, the coils were harvested and histological analysis was performed. SEM photographs showed that SF/SDF-1α-coated coils have uniform size and a thin film compared with bare coils. In the group treated with SDF-1α-coated coils, tissue organization was accelerated and the proliferation of α-smooth muscle actin positive cells was promoted in the aneurysmal sac. Compared with unmodified coils, on day 28, tissue organization was significantly greater in the group treated with SDF-1α-coated coils and MSC or EPC transplantation. These results suggest that SDF-1α-coated coils with MSC or EPC transplantation may be beneficial in the aneurysm healing and endothelialization at the orifice of embolized aneurysm.
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.|b Graduate School of Southern Medical University , Guangzhou , China.|c Department of Neurology , Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangdong Neuroscience Institute , Guangzhou , China.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.|b Graduate School of Southern Medical University , Guangzhou , China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Lu
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.|b Graduate School of Southern Medical University , Guangzhou , China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Chen
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.|b Graduate School of Southern Medical University , Guangzhou , China.
authors.forename: Xubo
authors.initials: X
authors.lastname: Cui
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.|b Graduate School of Southern Medical University , Guangzhou , China.
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.|b Graduate School of Southern Medical University , Guangzhou , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zheng
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: d School of Chemistry and Chemical Engineering , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zeng
authors.affiliation: d School of Chemistry and Chemical Engineering , Sun Yat-Sen University , Guangzhou , China.
authors.forename: Daping
authors.initials: D
authors.lastname: Quan
authors.affiliation: a The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital , Southern Medical University , Guangzhou , China.
authors.forename: Qiujing
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000783:Aneurysm / Q000628:therapy*; D000818:Animals; D054377:Chemokine CXCL12 / Q000378:metabolism / Q000627:therapeutic use*; D020099:Coated Materials, Biocompatible / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004621:Embolization, Therapeutic; D066026:Endothelial Progenitor Cells / Q000637:transplantation* / Q000648:ultrastructure; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D054377:Chemokine CXCL12; D020099:Coated Materials, Biocompatible
keywords: Coil; Endothelial progenitor cells; Intracranial aneurysm; Mesenchymal stem cells; Silk fibroin; Stromal cell-derived factor-1α