title: Amperometric biosensor based on Laccase immobilized onto a screen-printed electrode by Matrix Assisted Pulsed Laser Evaporation.
abstract: A Laccase-based biosensor for the determination of phenolic compounds was developed by using Matrix Assisted Pulsed Laser Evaporation as an innovative enzyme immobilization technique. and the deriving biosensor was characterized and applied for the first time. Laccase was immobilized onto different substrates including screen printed carbon electrodes and spectroscopic, morphologic and electrochemical characterizations were carried out. A linear range from 1 to 60μM was achieved working at 5.5pH and -0.2V detection potential vs Ag pseudoreference. The limits of detection and quantification were found to be 1 and 5μM, respectively. A good fabrication reproducibility, stability of response and selectivity toward interferents were also found The potential of the developed biosensor was tested in the determination of total polyphenol content in real matrices (tea infusion, ethanolic extract from Muscari comosum bulbs and aqueous solution of a food supplement from black radish root and artichoke leaves) and the results were compared with those obtained by using the Folin-Ciocalteu method.
authors.affiliation: Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy; Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche, c/da S. Loja, 85050 Tito Scalo (PZ), Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Verrastro
authors.affiliation: Istituto di Metodologie per l'Analisi Ambientale, Consiglio Nazionale delle Ricerche, c/da S. Loja, 85050 Tito Scalo (PZ), Italy. Electronic address: nunzia.cicco@imaa.cnr.it.
authors.forename: Nunzia
authors.initials: N
authors.lastname: Cicco
authors.affiliation: Istituto di Metodologie per l'Analisi Ambientale, Consiglio Nazionale delle Ricerche, c/da S. Loja, 85050 Tito Scalo (PZ), Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Crispo
authors.affiliation: Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche, c/da S. Loja, 85050 Tito Scalo (PZ), Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Morone
authors.affiliation: Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, Str. Atomistilor 409, 077125 Bucharest-Magurele, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Dinescu
authors.affiliation: Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, Str. Atomistilor 409, 077125 Bucharest-Magurele, Romania.
authors.forename: Marius
authors.initials: M
authors.lastname: Dumitru
authors.affiliation: Dipartimento di Biotecnologie, Università degli Studi di Verona, Strada Le Grazie 15, 37134 Verona, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Favati
authors.affiliation: Istituto di Metodologie per l'Analisi Ambientale, Consiglio Nazionale delle Ricerche, c/da S. Loja, 85050 Tito Scalo (PZ), Italy; Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, via Napoli 25, 71100 Foggia, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Centonze
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized; D042845:Laccase; D015203:Reproducibility of Results; D014835:Volatilization
chemical_list: D004800:Enzymes, Immobilized; D042845:Laccase
keywords: Biosensor; Laccase; Matrix Assisted Pulsed Laser Evaporation; Polyphenols assessment; Screen Printed Electrode