title: Study on cerebroprotective actions of Clerodendron glandulosumleaves extract against long term bilateral common carotid artery occlusion in rats.
abstract: Stroke is a major cause of death and disability worldwide. The resulting burden on the society continues to grow, with increase in the incidence of stroke. Oxidative stress has been involved in the pathogenesis of several neurological diseases including acute stroke.Focal and global cerebral ischemia represents diseases that are common in the human population.In recent years much attention is being paid towards the exploration of herbal preparation, antioxidant agents and combination therapies including COX-2 inhibitors in experimental model of stroke.Possible effect of a hydroalcoholic leaf extract of Clerodendron glandulosumColeb (C. glandulosum)on oxidant-antioxidant status in ischemia-hypoperfusion injury in the rat forebrain has been investigated.Healthy adult male Wistar albino rats were divided into five groups (n=8). Group I was served as Sham control (normal saline 1ml/kg, orally), group II was served hypoperfusion control (normal saline 1ml/kg, orally), group III, group IV were served as hydroalcoholic extract treated (200 and 400mg/kg, orally) and group V was treated with Quercetin (10mg/kg, orally) for 14days to assess preventive and curative effects of C. glandulosum. Flavonoid and phenolic compounds exhibit a broad spectrum of biological activity, including antioxidant. C. glandulosum extract (200 and 400mg/kg, p.o) was administered orally, once daily for a period of 2 weeks after the occlusion of BCCA. After 14th days rats were subjected to behavioral studies. After behavioral studies animals were sacrificed and brain was removed and homogenized. Estimation of Lipid peroxidation (LPO) Myeloperoxidase (MPO), estimation of protein levels and the activities of Superoxide dismutase (SOD), Catalase (CAT), were performed. Infarct size and histopathological changes were observed in treated groups.
authors.affiliation: Department of Pharmacology, Acharya & B.M. Reddy College of Pharmacy, Bangalore, 560 107, Karnataka, India.
authors.forename: Saritha
authors.initials: S
authors.lastname: Surapaneni
authors.affiliation: Department of Pharmacology, Acharya & B.M. Reddy College of Pharmacy, Bangalore, 560 107, Karnataka, India. Electronic address: prakasht@acharya.ac.in.
authors.forename: T
authors.initials: T
authors.lastname: Prakash
authors.affiliation: Department of Pharmacology, Acharya & B.M. Reddy College of Pharmacy, Bangalore, 560 107, Karnataka, India.
authors.forename: MdAsif
authors.initials: M
authors.lastname: Ansari
authors.affiliation: Department of Pharmacology, Acharya & B.M. Reddy College of Pharmacy, Bangalore, 560 107, Karnataka, India.
authors.forename: P M
authors.initials: PM
authors.lastname: Manjunath
authors.affiliation: Biochemistry, Indian Institute of Science, Bangalore, 560 012. Karnataka, India.
authors.forename: D
authors.initials: D
authors.lastname: Kotresha
authors.affiliation: Department of Pharmacology, Acharya & B.M. Reddy College of Pharmacy, Bangalore, 560 107, Karnataka, India.
authors.forename: Divakar
authors.initials: D
authors.lastname: Goli
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001157:Arterial Occlusive Diseases / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D001522:Behavior, Animal / Q000187:drug effects; D020520:Brain Infarction / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology; D017536:Carotid Artery, Common / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000187:drug effects; D032409:Clerodendrum / Q000737:chemistry*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D064209:Phytochemicals / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D013550:Swimming
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D064209:Phytochemicals; D010936:Plant Extracts
keywords: C. glandulosum; Cerebral ischemia; Cerebroprotective; Infarct size; Oxidative stress