title: Antifungal activity of chitinase obtained from Paenibacillus ehimensis MA2012 against conidial of Collectotrichum gloeosporioides in vitro.
abstract: To investigate the expression patterns of chitinase on SDS-PAGE gel, Paenibacillus ehimensis MA2012 was incubated in gelatin-chitin medium (GCM) at 30 °C for 7 days. Six major bands (Ch3, Ch4, Ch5, Ch6, Ch7, and Ch8) of chitinase isozymes in GC medium appeared on SDS-PAGE gel during the incubation period. Chitinase activity staining of P. ehimensis MA2012 was detected on 2-DE with different pI values (4-11). After DEAE-Sephadex chromatography, eight bands (Ch1 to Ch8) of chitinase isozymes were stained strongly with Calcofluor white M2R at fraction 45. After Sephadex G-75 gel filtration, six bands (Ch3 to Ch8) of chitinase isozymes were stained with Calcofluor white M2R at fractions of 11-12. The specific activity of the purified chitinase was 3.8 units mg(-1) protein with a purification factor of 0.27. Inhibition rate of the conidial germination of Colletotrichum gloeosporioides was 87% in partial purified chitinase treatment compared with control.
authors.affiliation: Fermented Food Science Division, Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Jeollabuk-do, 55365, South Korea.
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: Seo
authors.affiliation: Department of Agriculture Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju, 61186, South Korea.
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Agriculture Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju, 61186, South Korea.
authors.forename: Kil-Yong
authors.initials: KY
authors.lastname: Kim
authors.affiliation: Department of Agriculture Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju, 61186, South Korea. Electronic address: woojung@jnu.ac.kr.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Jung
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism*; D002688:Chitinases / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D020231:Colletotrichum / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D056507:Paenibacillus / Q000201:enzymology* / Q000254:growth & development; D013172:Spores, Fungal / Q000187:drug effects*; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D007527:Isoenzymes; D002688:Chitinases
keywords: Chitinase activity; Colletotrichum gloeosporioides; Isozyme; Paenibacillus ehimensis