title: Ultrasensitive enrichment of phosphopeptides with Ti(4+) immobilized SiO2 graphene-like multilayer nanosheets.
abstract: Ti(4+) immobilized SiO2 graphene-like multilayer nanosheets were designed and synthesized for the ultrasensitive enrichment of phosphopeptides with the detection limit of 1.0 pg μL(-1) (4 × 10(-17) mol μL(-1)). The enrichment selectivity of the nanosheets was evaluated by capturing phosphopeptides from a peptide mixture of bovine β-casein (β-casein) and bovine serum albumin (BSA) with the molar ratio of 1 : 1000 (1.7 × 10(-12) mol of β-casein and 1.7 × 10(-9) mol of BSA in 100 μL).
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. xmzhang@fudan.edu.cn.
authors.forename: Dongpo
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. xmzhang@fudan.edu.cn.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. xmzhang@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. xmzhang@fudan.edu.cn.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002364:Caseins / Q000032:analysis; D002417:Cattle; D006108:Graphite; D049329:Nanostructures; D010748:Phosphopeptides / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D012822:Silicon Dioxide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium
chemical_list: D002364:Caseins; D010748:Phosphopeptides; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D006108:Graphite; D014025:Titanium