title: Effects of antioxidants, catalase and α-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells.
abstract: Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P ≤ 0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the antioxidant groups. ROS production with antioxidant was significantly lower compared with the control group. Cryopreservation with the addition of the antioxidants can help increase the number of SSCs and improve the quality and viability of these cells after cryopreservation.
authors.affiliation: a Department of Anatomy, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Freshte
authors.initials: F
authors.lastname: Aliakbari
authors.affiliation: b Department of Urology, Shariati Hospital , Tehran University of Medical Sciences , Tehran , Iran.|c Department of Andrology, Reproductive Biomedicine Research Center , Royan Institute for Stem Cell Biology and Technology, ACECR , Tehran , Iran.
authors.forename: Mohamad Ali
authors.initials: MA
authors.lastname: Sedighi Gilani
authors.affiliation: a Department of Anatomy, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Yazdekhasti
authors.affiliation: f Cellular and Molecular Research Center & Department of Anatomical Sciences , Iran University of Medical Sciences , Tehran , Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Koruji
authors.affiliation: g Department of Anatomical Sciences, Faculty of Medicine , Iran University of Medical Sciences , Tehran , Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Asgari
authors.affiliation: d Department of Anatomy, School of Medicine , Arak University of Medical Sciences , Arak , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Baazm
authors.affiliation: e PrimeGen Biotech LLC , Santa Ana , CA , USA.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Izadyar
authors.affiliation: a Department of Anatomy, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Kharrazi Nejad
authors.affiliation: a Department of Anatomy, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Khanezad
authors.affiliation: a Department of Anatomy, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Abbasi
mesh_terms: D000072956:Adult Germline Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation; D005615:Freezing; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002374:Catalase; D024502:alpha-Tocopherol
keywords: Catalase; cryopreservation; spermatogonial stem cell; α-tocopherol