title: Induction Effect of Bisphenol A on Gene Expression Involving Hepatic Oxidative Stress in Rat.
abstract: Background and Objective. Bisphenol A (BPA) is an abundantly used xenoestrogenic chemical which may cause various disorders in body. In the present study, we sought to investigate the effects of various doses of BPA on hepatic oxidative stress-related gene expression in rats. Methods. Male Wistar rats weighing 150-200 g were used in this study. Three doses of the BPA (5, 25, and 125 μg/kg) in corn oil were administered as gavage during 35 consecutive days. After the experiment, the rats were expired and the livers were removed and stored at -80°C freezer for RNA extraction. Findings. The Real Time PCR showed increased expression of HO-1 in the rats receiving BPA doses compared to the control group. This effect was dose-dependent and higher at doses of 25 and 125 μg/kg than 5 μg/kg of body weight (p < 0.05). It was also demonstrated that various doses BPA can increase GADD45B gene expression compared to control group. That expression was significantly dominant in the lowest dose (5 μg/kg) of the BPA (p < 0.05). The final body weights (168.0 ± 10.0 gr) in the treatment group [BPA (125 μg/kg)] showed a significant decrease compared to control group (191.60 ± 6.50 gr). Conclusion. These findings demonstrate that BPA generated ROS and increased the antioxidant gene expression that causes hepatotoxicity.
authors.affiliation: Cellular and Molecular biology research centre, health research institute, Babol University of Medical Sciences, Babol, Iran; Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Sohrab
authors.initials: S
authors.lastname: Kazemi
authors.affiliation: Cellular and Molecular biology research centre, health research institute, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Seydeh Narges
authors.initials: SN
authors.lastname: Mousavi
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Aghapour
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Boshra
authors.initials: B
authors.lastname: Rezaee
authors.affiliation: Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Farzin
authors.initials: F
authors.lastname: Sadeghi
authors.affiliation: Cellular and Molecular biology research centre, health research institute, Babol University of Medical Sciences, Babol, Iran; Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Moghadamnia
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D010636:Phenols / Q000633:toxicity*; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D000097642:GADD45 Proteins; D000098826:Bisphenol A Compounds
chemical_list: D000943:Antigens, Differentiation; D001559:Benzhydryl Compounds; D051547:Heme Oxygenase-1; D010636:Phenols; C508094:Gadd45b protein, rat; D000097642:GADD45 Proteins; C006780:bisphenol A; D000098826:Bisphenol A Compounds