title: Evaluation of electrospun PLLA/PEGDMA polymer coatings for vascular stent material.
abstract: The field of percutaneous coronary intervention has seen a plethora of advances over the past few decades, which have allowed for its development into safe and effective treatments for patients suffering from cardiovascular diseases. However, stent thrombosis and in-stent restenosis remain clinically significant problems. Herein, we describe the synthesis and characterization of fibrous polymer coatings on stent material nitinol, in the hopes of developing a more suitable stent surface to enhance re-endothelialization. Electrospinning technique was used to fabricate polyethylene glycol dimethacrylate/poly l-lactide acid (PEGDMA/PLLA) blend fiber substrate with tunable elasticity and hydrophilicity for use as coatings. Attachment of platelets and arterial smooth muscle cells (SMC) onto the coatings as well as the secretory effect of mesenchymal stem cells cultured on the coatings on the proliferation and migration of arterial endothelial cells and SMCs were assessed. It was demonstrated that electrospun PEGDMA/PLLA coating with 1:1 ratio of the components on the nitinol stent-reduced platelet and SMC attachment and increased stem cell secretory factors that enhance endothelial proliferation. We therefore postulate that the fibrous coating surface would possess enhanced biological compatibility of nitinol stents and hold the potential in preventing stent failure through restenosis and thrombosis.
authors.affiliation: a Department of Mechanical Engineering , University of Colorado at Boulder , Boulder , CO , USA.|b Department of Civil Engineering , University of Colorado at Boulder , Boulder , CO , USA.
authors.forename: Parnaz
authors.initials: P
authors.lastname: Boodagh
authors.affiliation: c Department of Bio-inspired Structure and Surface Engineering , Nanjing University of Aeronautics and Astronautics , Nanjing City , P. R. China.
authors.forename: Dong-Jie
authors.initials: DJ
authors.lastname: Guo
authors.affiliation: a Department of Mechanical Engineering , University of Colorado at Boulder , Boulder , CO , USA.
authors.forename: Naveen
authors.initials: N
authors.lastname: Nagiah
authors.affiliation: a Department of Mechanical Engineering , University of Colorado at Boulder , Boulder , CO , USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
mesh_terms: D000497:Alloys; D000818:Animals; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000502:physiology; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004548:Elasticity; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D032389:Myocytes, Smooth Muscle / Q000502:physiology; D057139:Nanofibers / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011651:Pulmonary Artery / Q000166:cytology; D051381:Rats; D015607:Stents; D013499:Surface Properties
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D011091:Polyesters; C421283:poly(ethylene glycol)-dimethacrylate; C013616:nitinol; D011092:Polyethylene Glycols; C033616:poly(lactide)
keywords: Stent coating; biomaterials; electrospin; nanofibrous materials; polymers; thrombosis