title: Triggering bilayer to inverted-hexagonal nanostructure formation by thiol-ene click chemistry on cationic lipids: consequences on gene transfection.
abstract: The ramification of cationic amphiphiles on their unsaturated lipid chains is readily achieved by using the thiol-ene click reaction triggering the formation of an inverted hexagonal phase (HII). The new ramified cationic lipids exhibit different bio-activities (transfection, toxicity) including higher transfection efficacies on 16HBE 14o-cell lines.
authors.affiliation: CEMCA CNRS UMR 6521, plateforme de RMN RPE MS, Université de Brest, IBSAM, 6 Avenue V. Le Gorgeu, 29238 Brest, France. pjaffres@univ-brest.fr.
authors.forename: Damien
authors.initials: D
authors.lastname: Afonso
authors.affiliation: INSERM U1078, Université de Brest, IBSAM, Faculté de Médecine et des sciences de la santé, 22 avenue Camille Desmoulins, 29238 Brest Cedex 3, France.
authors.forename: Tony
authors.initials: T
authors.lastname: Le Gall
authors.affiliation: CEMCA CNRS UMR 6521, plateforme de RMN RPE MS, Université de Brest, IBSAM, 6 Avenue V. Le Gorgeu, 29238 Brest, France. pjaffres@univ-brest.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Couthon-Gourvès
authors.affiliation: Institute of Chemistry & Biology of Membranes & Nano-objects, CBMN, UMR CNRS 5248, University of Bordeaux, Bordeaux INP, Allée Geoffroy de St Hilaire, F-33600 Pessac, France.
authors.forename: Axelle
authors.initials: A
authors.lastname: Grélard
authors.affiliation: Institute of Chemistry & Biology of Membranes & Nano-objects, CBMN, UMR CNRS 5248, University of Bordeaux, Bordeaux INP, Allée Geoffroy de St Hilaire, F-33600 Pessac, France.
authors.forename: Shipra
authors.initials: S
authors.lastname: Prakash
authors.affiliation: CEMCA CNRS UMR 6521, plateforme de RMN RPE MS, Université de Brest, IBSAM, 6 Avenue V. Le Gorgeu, 29238 Brest, France. pjaffres@univ-brest.fr.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Berchel
authors.affiliation: CEMCA CNRS UMR 6521, plateforme de RMN RPE MS, Université de Brest, IBSAM, 6 Avenue V. Le Gorgeu, 29238 Brest, France. pjaffres@univ-brest.fr.
authors.forename: Nelly
authors.initials: N
authors.lastname: Kervarec
authors.affiliation: Institute of Chemistry & Biology of Membranes & Nano-objects, CBMN, UMR CNRS 5248, University of Bordeaux, Bordeaux INP, Allée Geoffroy de St Hilaire, F-33600 Pessac, France.
authors.forename: Erick J
authors.initials: EJ
authors.lastname: Dufourc
authors.affiliation: INSERM U1078, Université de Brest, IBSAM, Faculté de Médecine et des sciences de la santé, 22 avenue Camille Desmoulins, 29238 Brest Cedex 3, France and CHRU de Brest, Hôpital Morvan, 5 avenue du maréchal Foch, 29609 Brest cedex, France and DUMG, Faculté de Médecine et des Sciences de la Santé, 22 avenue Camille Desmoulins, 29238 Brest cedex 3, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Montier
authors.affiliation: CEMCA CNRS UMR 6521, plateforme de RMN RPE MS, Université de Brest, IBSAM, 6 Avenue V. Le Gorgeu, 29238 Brest, France. pjaffres@univ-brest.fr.
authors.forename: Paul-Alain
authors.initials: PA
authors.lastname: Jaffrès
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D002460:Cell Line; D057930:Click Chemistry; D004337:Drug Carriers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014162:Transfection
chemical_list: D000475:Alkenes; D004337:Drug Carriers; D008055:Lipids; D013438:Sulfhydryl Compounds