title: Mycobacterial antigen 85 complex (Ag85) as a target for ficolins and mannose-binding lectin.
abstract: The pattern recognition molecules (PRMs) able to activate complement via the lectin pathway are suspected to be involved in the interaction between pathogenic Mycobacteria and the host immune response. Recently, we have found strong interactions between 25 and 35kDa mycobacterial cell fractions and mannose-binding lectin (MBL) and ficolins. Here we demonstrate that two biologically important mycobacterial structures, mannosylated lipoarabinomannan (ManLAM) and the antigen 85 (Ag85) complex, induce activation of the lectin pathway of complement. The strong interaction of recombinant MBL with purified ManLAM was confirmed, but no binding of recombinant ficolins (ficolin-1, -2, -3) with this structure was observed. Interestingly, all PRMs tested reacted with the mycobacterial antigen 85 (Ag85) complex. Based on the use of specific inhibitors (mannan for MBL, acetylated bovine serum albumin for ficolin-1 and -2, Hafnia alvei PCM 1200 lipopolysaccharide for ficolin-3), we concluded that carbohydrate-recognition (MBL) and fibrinogen-like domains (ficolins) were involved in these interactions. Our results indicate that the mycobacterial antigen 85 complex is a target for ficolins and MBL. Furthermore, those PRMs also bound to fibronectin and therefore might influence the Ag85 complex-dependent interaction of Mycobacterium with the extracellular matrix.
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Świerzko
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland.
authors.forename: Marcin A
authors.initials: MA
authors.lastname: Bartłomiejczyk
authors.affiliation: Laboratory of Mycobacterium Genetics and Physiology, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Brzostek
authors.affiliation: Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Łukasiewicz
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Michalski
authors.affiliation: Laboratory of Mycobacterium Genetics and Physiology, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Dziadek
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 Lodz, Poland. Electronic address: mcedzynski@cbm.pan.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Cedzyński
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D051379:Mice; D009161:Mycobacterium / Q000276:immunology*; D000097604:Ficolins
chemical_list: D000217:Acyltransferases; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D037102:Lectins; D008070:Lipopolysaccharides; D037601:Mannose-Binding Lectin; D000097604:Ficolins; C050016:lipoarabinomannan; C112580:antigen 85A, Mycobacterium tuberculosis; C121433:antigen 85B, Mycobacterium tuberculosis
keywords: Antigen 85 (Ag85) complex; Ficolin; Lipoarabinomannan (LAM); Mannose-binding lectin (MBL); Mycobacterium