title: Effect of ozonolysis pretreatment parameters on the sugar release, ozone consumption and ethanol production from sugarcane bagasse.
abstract: A L9(3)(4) orthogonal array (OA) experimental design was applied to study the four parameters considered most important in the ozonolysis pretreatment (moisture content, ozone concentration, ozone/oxygen flow and particle size) on ethanol production from sugarcane bagasse (SCB). Statistical analysis highlighted ozone concentration as the highest influence parameter on reaction time and sugars release after enzymatic hydrolysis. The increase on reaction time when decreasing the ozone/oxygen flow resulted in small differences of ozone consumptions. Design optimization for sugars release provided a parameters combination close to the best experimental run, where 77.55% and 56.95% of glucose and xylose yields were obtained, respectively. When optimizing the grams of sugar released by gram of ozone, the highest influence parameter was moisture content, with a maximum yield of 2.98gSUGARS/gO3. In experiments on hydrolysates fermentation, Saccharomyces cerevisiae provided ethanol yields around 80%, while Pichia stipitis was completely inhibited.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid - UVa, Calle Doctor Mergelina, s/n, 47005 Valladolid, Spain. Electronic address: rtravaini@gmail.com.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Travaini
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid - UVa, Paseo de Belén, 7, 47011 Valladolid, Spain. Electronic address: ebarrado@qa.uva.es.
authors.forename: Enrique
authors.initials: E
authors.lastname: Barrado
authors.affiliation: Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid - UVa, Calle Doctor Mergelina, s/n, 47005 Valladolid, Spain. Electronic address: silvia@iq.uva.es.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bolado-Rodríguez
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000494:pharmacology; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010126:Ozone / Q000737:chemistry* / Q000378:metabolism; D010843:Pichia / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D000431:Ethanol; D010126:Ozone; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D005947:Glucose; D010100:Oxygen
keywords: Enzymatic hydrolysis; Ethanol; Experimental design; Ozonolysis pretreatment; Sugarcane bagasse