title: Hydroxytyrosol and its complex forms (secoiridoids) modulate aorta and heart proteome in healthy rats: Potential cardio-protective effects.
abstract: Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in both free and complex forms (secoiridoids; SEC). Proteomics of cardiovascular tissues such as aorta or heart represents a promising tool to uncover the mechanisms of action of phenolic compounds in healthy animals.
  
  
  
  Twelve female Wistar rats were separated into three groups: a standard diet and two diets supplemented in phenolic compounds (HT and SEC) adjusted to 5 mg/kg/day during 21 days. Proteomic analyses of aorta and heart tissues were performed by nano-LC and MS. Ingenuity Pathway Analysis was used to generate interaction networks. HT or SEC modulated aorta and heart proteome compared to the standard diet. The top-scored networks were related to Cardiovascular System. HT and SEC downregulated proteins related to proliferation and migration of endothelial cells and occlusion of blood vessels in aorta and proteins related to heart failure in heart tissue. SEC showed higher fold change values compared to HT, attributed to higher concentration of HT detected in heart tissue.
  
  
  
  Changes at proteomic level in cardiovascular tissues may partially account for the underlying mechanisms of VOO phenols cardiovascular protection being the SEC effects higher than free HT.
authors.affiliation: Functional Nutrition, Oxidation and Cardiovascular Diseases Group (NFOC-Salut), Unit of Lipids and Atherosclerosis Research (URLA), Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitari Sant Joan, IISPV, Technological Center of Nutrition and Health (CTNS), Faculty of Medicine and Health Sciences, Universitat Rovira i Virgili, Sant Llorenç, Reus, Spain.
authors.forename: Úrsula
authors.initials: Ú
authors.lastname: Catalán
authors.affiliation: Functional Nutrition, Oxidation and Cardiovascular Diseases Group (NFOC-Salut), Unit of Lipids and Atherosclerosis Research (URLA), Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitari Sant Joan, IISPV, Technological Center of Nutrition and Health (CTNS), Faculty of Medicine and Health Sciences, Universitat Rovira i Virgili, Sant Llorenç, Reus, Spain.|Food Technology Department, Universitat de Lleida-AGROTECNIO Center, Lleida, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Rubió
authors.affiliation: Food Technology Department, Universitat de Lleida-AGROTECNIO Center, Lleida, Spain.
authors.forename: Maria-Carmen
authors.initials: MC
authors.lastname: López de Las Hazas
authors.affiliation: Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Reus, Spain.
authors.forename: Pol
authors.initials: P
authors.lastname: Herrero
authors.affiliation: Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Reus, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Nadal
authors.affiliation: Centre for Omic Sciences, Universitat Rovira i Virgili (COS-URV), Reus, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Canela
authors.affiliation: Functional Nutrition, Oxidation and Cardiovascular Diseases Group (NFOC-Salut), Unit of Lipids and Atherosclerosis Research (URLA), Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitari Sant Joan, IISPV, Technological Center of Nutrition and Health (CTNS), Faculty of Medicine and Health Sciences, Universitat Rovira i Virgili, Sant Llorenç, Reus, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Pedret
authors.affiliation: Food Technology Department, Universitat de Lleida-AGROTECNIO Center, Lleida, Spain.
authors.forename: Maria-José
authors.initials: MJ
authors.lastname: Motilva
authors.affiliation: Functional Nutrition, Oxidation and Cardiovascular Diseases Group (NFOC-Salut), Unit of Lipids and Atherosclerosis Research (URLA), Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Hospital Universitari Sant Joan, IISPV, Technological Center of Nutrition and Health (CTNS), Faculty of Medicine and Health Sciences, Universitat Rovira i Virgili, Sant Llorenç, Reus, Spain. rosa.sola@urv.cat.
authors.forename: Rosa
authors.initials: R
authors.lastname: Solà
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology*; D019587:Dietary Supplements; D005260:Female; D006321:Heart / Q000187:drug effects*; D039823:Iridoids / Q000494:pharmacology*; D009206:Myocardium / Q000378:metabolism; D000069463:Olive Oil / Q000737:chemistry / Q000494:pharmacology; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D020543:Proteome / Q000378:metabolism*; D017208:Rats, Wistar
chemical_list: D002316:Cardiotonic Agents; D039823:Iridoids; D000069463:Olive Oil; D010936:Plant Extracts; D020543:Proteome; C005975:3,4-dihydroxyphenylethanol; D010626:Phenylethyl Alcohol
keywords: Cardiovascular disease; Healthy rats; Hydroxytyrosol; Proteome; Secoiridoids