title: Kelvin probe force microscopy of DNA-capped nanoparticles for single-nucleotide polymorphism detection.
abstract: Kelvin probe force microscopy (KPFM) is a robust toolkit for profiling the surface potential (SP) of biomolecular interactions between DNAs and/or proteins at the single molecule level. However, it has often suffered from background noise and low throughput due to instrumental or environmental constraints, which is regarded as limiting KPFM applications for detection of minute changes in the molecular structures such as single-nucleotide polymorphism (SNP). Here, we show KPFM imaging of DNA-capped nanoparticles (DCNP) that enables SNP detection of the BRCA1 gene owing to sterically well-adjusted DNA-DNA interactions that take place within the confined spaces of DCNP. The average SP values of DCNP interacting with BRCA1 SNP were found to be lower than the DCNP reacting with normal (non-mutant) BRCA1 gene. We also demonstrate that SP characteristics of DCNP with different substrates (e.g., Au, Si, SiO2, and Fe) provide us with a chance to attenuate or augment the SP signal of DCNP without additional enhancement of instrumentation capabilities.
authors.affiliation: Department of Biomedical Engineering, Yonsei University, Wonju 26493, Korea. yusuklee@yonsei.ac.kr.
authors.forename: Hyungbeen
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Bio-convergence Engineering, Korea University, Seoul 02841, Korea. dsyoon@korea.ac.kr.
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Lee
authors.affiliation: School of Public Health, Harvard University, Boston, Massachusetts 02115, USA.
authors.forename: Gyudo
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Yonsei University, Wonju 26493, Korea. yusuklee@yonsei.ac.kr.
authors.forename: Wonseok
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Electrical Engineering, Kwangwoon University, Seoul 01897, Korea.
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Center for Biomicrosystem, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.
authors.forename: Kyo Seon
authors.initials: KS
authors.lastname: Hwang
authors.affiliation: Department of Radiology, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Biomedical Engineering, Yonsei University, Wonju 26493, Korea. yusuklee@yonsei.ac.kr.
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Department of Bio-convergence Engineering, Korea University, Seoul 02841, Korea. dsyoon@korea.ac.kr.
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Yoon
mesh_terms: D004247:DNA / Q000032:analysis*; D019398:Genes, BRCA1; D006801:Humans; D018625:Microscopy, Atomic Force; D053758:Nanoparticles; D020641:Polymorphism, Single Nucleotide; D012822:Silicon Dioxide
chemical_list: D012822:Silicon Dioxide; D004247:DNA