title: Niche nanoparticle-based FRET assay for bleomycin detection via DNA scission.
abstract: We describe a highly sensitive nanoparticle-based fluorescence resonance energy transfer (FRET) probe developed without using molecular fluorophores as donors and acceptors. The success of this work relies on the strategy that DNA scission was designed to occur to the probe when target presented, which enabled the fluorescence signal "turn-on" of graphene quantum dots (GQDs) and thus quantitative analysis. In particular, amino-modified SiO2 NPs were initially coated by GQDs to form highly emitting SiO2/GQDs, followed by conjunction with DNA functionalized gold nanoparticles (Au NPs-DNA) to form SiO2/GQDs-DNA-Au NPs composite. Owing to the FRET interactions between the GQDs and Au NPs, the fluorescence of GQDs was effectively quenched by Au NPs. When bleomycin (BLM), a model analyte, was mixed with the probe, the fluorescence signal of GQDs would be restored due to the removal of Au NPs from the SiO2/GQDs surface by DNA scission treatment with BLM in the presence of Fe (II). The current FRET probe shows a good linear relationship between the fluorescence intensity and the concentration of BLM in the range from 0.5nM to 1μM with a detection limit of 0.2nM. The probe also shows satisfactory results for the analysis of clinical serum samples. This method provides versatility to the application of GQDs in FRET biosensing and could be potentially extended to other similar systems by replacing the linker between the GQDs and Au NPs.
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Haimeng
authors.initials: H
authors.lastname: Pei
authors.affiliation: National Engineering Research Center for Colloidal Materials, Shandong University, Jinan, Shandong 250100, PR China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Rongmei
authors.initials: R
authors.lastname: Kong
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xia
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China. Electronic address: fengliquhn@hotmail.com.
authors.forename: Fengli
authors.initials: F
authors.lastname: Qu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000032:analysis / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods; D001761:Bleomycin / Q000032:analysis / Q000097:blood*; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots / Q000737:chemistry / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; D006046:Gold; D012822:Silicon Dioxide; D006108:Graphite; D004247:DNA
keywords: Bleomycin; Fluorescence resonance energy transfer; Gold nanoparticles; Graphene oxide quantum dots; Silica nanospheres