title: Prophylactic effects of pomegranate (Punica granatum) juice on sodium fluoride induced oxidative damage in liver and erythrocytes of rats.
abstract: The objective of this study was to investigate the protective effects of pomegranate (Punica granatum) juice (PGJ) on oxidative damages in liver tissue and erythrocytes of rats intoxicated by sodium fluoride (NaF). Rats were randomly divided into two groups: group I received standard diet and group II received orally 1 mL of PGJ. After 5 weeks of pretreatment, each group was divided again into two subgroups and treated for another 3 weeks as follows: group I was subdivided into a control group and a group that was treated with 100 ppm of NaF (in drinking water); group II was subdivided into one group that was treated daily with both 100 ppm NaF and PGJ (1 mL orally) and one that received daily 1 mL of pomegranate juice. Exposure to NaF decreased hematological parameters, changed the total protein, albumin, bilirubin levels, and increased the activities of hepatic marker enzymes. We also noted an increase in lipid peroxidation contents, accompanied by a decrease of reduced glutathione levels. Antioxidant enzyme activities in both tissues were modified in the NaF group compared with the control group. However, the administration of PGJ juice caused an amelioration of the previous parameters. Our results indicated the potential effects of NaF to induce oxidative damage in tissues and the ability of PGJ to attenuate NaF-induced oxidative injury.
authors.affiliation: a Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria.
authors.forename: Asma
authors.initials: A
authors.lastname: Bouasla
authors.affiliation: a Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria.
authors.forename: Ihcène
authors.initials: I
authors.lastname: Bouasla
authors.affiliation: a Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria.
authors.forename: Amel
authors.initials: A
authors.lastname: Boumendjel
authors.affiliation: b Laboratory of Animal Ecophysiology, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria.
authors.forename: Cherif
authors.initials: C
authors.lastname: Abdennour
authors.affiliation: c Laboratory of Animal Ecophysiology, Faculty of Sciences, Sfax, Soukra Road Km 3.5, BP 802, 3018 Sfax, Tunisia.
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.affiliation: a Laboratory of Biochemistry and Environmental Toxicology, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria.
authors.forename: Mahfoud
authors.initials: M
authors.lastname: Messarah
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D000067030:Fruit and Vegetable Juices; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D029561:Lythraceae; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D056990:Post-Exposure Prophylaxis / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D012969:Sodium Fluoride; D005978:Glutathione
keywords: Punica granatum; erythrocytes; fluorure de sodium; foie; liver; oxidative stress; rats; sodium fluoride; stress oxydatif; érythrocytes