title: Effects of nitrogen on the lipid and carotenoid accumulation of oleaginous yeast Sporidiobolus pararoseus.
abstract: Nitrogen limited but carbon excess condition was used to obtain high cellular lipid content and production. The maximum lipid production was 51 g/L, the lipid content in the dry cell was 60 %, and the lipid productivity was 0.53 g/L/h. In the fermentation, the content of lipid was raised from 20 % of dry cell weight to 60 %, and the proportion of oleic acid was raised from 66.8 to 72.5 %. Meanwhile, the metabolism of carotenoids switched to torulene, and its proportion was raised from 30 to 58 %. This was according to torulene had the better antioxidant ability than β-carotene to protect the strain from oxidative damage proved by their ABTS* radical scavenging activity and lipid peroxidation inhibition ability. Sporidiobolus pararoseus lipid was a good source of lipid not only because of its high oleic acid composition, but also the antioxidant ability of carotenoids in the lipid.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, 1800# Lihu Road, Wuxi, 214122, JiangSu, China.|State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy and Food Co., Ltd, 200436, Shanghai, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy and Food Co., Ltd, 200436, Shanghai, China.|Synergetic Innovation Center of Food Safety and Nutrition, 200436, Shanghai, China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, 1800# Lihu Road, Wuxi, 214122, JiangSu, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Du
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, 1800# Lihu Road, Wuxi, 214122, JiangSu, China.
authors.forename: He
authors.initials: H
authors.lastname: Qian
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, 1800# Lihu Road, Wuxi, 214122, JiangSu, China. hanmei.918521@163.com.
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001487:Basidiomycota / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D005285:Fermentation; D050356:Lipid Metabolism; D009584:Nitrogen / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002338:Carotenoids; D009584:Nitrogen
keywords: Biodiesel; Carotenoid; Oleaginous yeast; Sporidiobolus pararoseus