title: Ergosterols from the Culture Broth of Marine Streptomyces anandii H41-59.
abstract: An actinomycete strain, H41-59, isolated from sea sediment in a mangrove district, was identified as Streptomyces anandii on the basis of 16S rDNA gene sequence analysis as well as the investigation of its morphological, physiological and biochemical characteristics. Three new ergosterols, ananstreps A-C (1-3), along with ten known ones (4-13), were isolated from the culture broth of this strain. The gross structures of these new compounds were elucidated on the basis of extensive analysis of spectroscopic data, including HR-ESI-MS, and NMR. The cytotoxicities of these isolates against human breast adenocarcinoma cell line MCF-7, human glioblastoma cell line SF-268, and human lung cancer cell line NCI-H460 and their antibacterial activities in inhibiting the growth of Candida albicans and some other pathogenic microorganisms were tested. Compounds 3-8, 10 and 11 displayed cytotoxicity with IC50 values in a range from 13.0 to 27.8 μg/mL. However, all the tested compounds showed no activity on C. albicans and other bacteria at the test concentration of 1 mg/mL with the paper disc diffusion method.
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China. yangmzhang@sina.com.
authors.forename: Yang-Mei
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China. hongyu88926@126.com.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Li
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China. kitten_hc@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Hu
authors.affiliation: Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510632, China. shenhf@gdppri.com.
authors.forename: Hui-Fan
authors.initials: HF
authors.lastname: Sheng
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China. carolynzy@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510632, China. linbr@126.com.
authors.forename: Bi-Run
authors.initials: BR
authors.lastname: Lin
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China. guangxzh@sina.com.
authors.forename: Guang-Xiong
authors.initials: GX
authors.lastname: Zhou
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D045744:Cell Line, Tumor; D004875:Ergosterol / Q000737:chemistry* / Q000494:pharmacology; D019015:Geologic Sediments / Q000382:microbiology; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008826:Microbial Sensitivity Tests / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D004875:Ergosterol
keywords: Streptomyces anandii; ananstreps A–C; cytotoxicity; ergosterols; strain identification