title: [IDENTIFICATION OF THE INFECTIOUS PANCREATIC NECROSIS VIRUS (IPNV) USING THE ENZYME IMMUNOASSAY].
abstract: The infectious pancreatic necrosis (IPN) caused by a non-enveloped virus of the Birnaviridae family is one of the most important loss factors in the salmonid aquaculture. Virus isolation in the sensitive cell cultures has been approved in the Russian Federation as the diagnostic method for determination of IPNV antigen. This work gives the results of the development of the diagnostic test to reveal IPNV using the antigen-bound ELISA (sandwich ELISA). The developed test supplements a new diagnostic method and verifies some disputable results obtained with classical methods.
authors.forename: E A
authors.initials: EA
authors.lastname: Zavyalova
authors.forename: M I
authors.initials: MI
authors.lastname: Gulyukin
authors.forename: M A
authors.initials: MA
authors.lastname: Carpova
authors.forename: P D
authors.initials: PD
authors.lastname: Bogdanova
authors.forename: A E
authors.initials: AE
authors.lastname: Droshnev
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D018175:Birnaviridae Infections / Q000175:diagnosis* / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology; D007106:Immune Sera / Q000737:chemistry; D018061:Infectious pancreatic necrosis virus / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012474:Salmon / Q000821:virology; D014337:Trout / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D011933:Reagent Kits, Diagnostic