title: Nutrient Recovery of Starch Processing Waste to Cordyceps militaris: Solid State Cultivation and Submerged Liquid Cultivation.
abstract: This study demonstrated the potential for managing starch processing waste (SPW) by bioconversion to Cordyceps militaris mycelia using solid state cultivation (SSC) and submerged liquid cultivation (SLC). The growth characteristics of C. militaris mycelium were accessed and compared for SSC and SLC systems on SPW under various conditions of initial SPW concentration, pH, and operating temperature. To quantify the mycelial biomass in SLC, original primer sets targeting the 18S rRNA gene of C. militaris were developed. In SSC, a maximum mycelial growth rate (543.1 mm(2)/day) was predicted to occur at 25.6 g SPW/L, pH 5.5, and 23.8 °C. In SLC, a maximum mycelial growth rate (1918.6 mg/L/day) was predicted to occur at 35.5 g SPW/L, pH 5.5, and 22.0 °C. Temperature was suggested as the most significant factor in both systems. The higher optimum substrate concentration observed for SLC than for SSC was likely due to difference in mycelial morphology and mixing effect.
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Joonyeob
authors.initials: J
authors.lastname: Lee
authors.affiliation: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, 39-1 Hawolgok-Dong, Seongbuk-Gu, Seoul, 136-791, Republic of Korea.
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Cho
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Seung Gu
authors.initials: SG
authors.lastname: Shin
authors.affiliation: Center for Water Resource Cycle Research, Korea Institute of Science and Technology, 39-1 Hawolgok-Dong, Seongbuk-Gu, Seoul, 136-791, Republic of Korea.
authors.forename: Hyokwan
authors.initials: H
authors.lastname: Bae
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Taewoan
authors.initials: T
authors.lastname: Koo
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Gyuseong
authors.initials: G
authors.lastname: Han
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea. shwang@postech.ac.kr.
authors.forename: Seokhwan
authors.initials: S
authors.lastname: Hwang
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D032661:Cordyceps / Q000254:growth & development / Q000378:metabolism*; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000302:isolation & purification*; D010758:Phosphorus / Q000302:isolation & purification*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013213:Starch / Q000378:metabolism*; D013696:Temperature; D014866:Waste Products
chemical_list: D012337:RNA, Ribosomal, 18S; D014866:Waste Products; D010758:Phosphorus; D013213:Starch; D009584:Nitrogen
keywords: Bioconversion; Cordyceps militaris; Fungal process; Real-time quantitative polymerase chain reaction (QPCR); Response surface analysis (RSA)