title: Facile synthesis of Cu(2+)-modified mesoporous silica-coated magnetic graphene composite for enrichment of microcystin-LR followed by mass spectrometry analysis.
abstract: MCs is a group of potent hepatotoxic peptides produced by cyanobacterial in eutrophic water, among which microcystin-LR is the most abundant and toxic. Long-time accumulation of even trace dosage from drinking water would cause significantly hepatic injury to animal and humans. Here we reported a novel Cu(2+)-modified mesoporous silica coated magnetic graphene composite (magG@mSiO2@-Cu(2+)) through mild sol-gel process and surface modification. Next, the composites were successfully applied for enrichment and separation of microcystin-LR followed by MALDI-TOF MS analysis based on the virtues of excellent hydrophilicity, high surface area (261cm(2)g(-1)), sensitively magnetic separation property, accessible porosity (3.10nm) and large amount of modified Cu(2+) ions. Even performed in a lower concentration (0.5μg/L), at which microcystin-LR could not be detected directly, after treatment with the composites the S/N ratio could appear to be 82.93. Furthermore, the novel composites also exhibited high enrichment efficiency in real water sample. It provided a sensitive and efficient technique for enrichment and detection of microcystin-LR and developed a potent method for separation of pollutant in contaminated water.
authors.affiliation: Department of Chemistry & Institutes of Biomedical Science, Fudan University, Shanghai 200433, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Chemistry & Institutes of Biomedical Science, Fudan University, Shanghai 200433, China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.affiliation: Department of Chemistry & Institutes of Biomedical Science, Fudan University, Shanghai 200433, China.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006108:Graphite; D006801:Humans; D008280:Magnetics; D008387:Marine Toxins; D013058:Mass Spectrometry; D052998:Microcystins; D016062:Porosity; D012822:Silicon Dioxide
chemical_list: D008387:Marine Toxins; D052998:Microcystins; D012822:Silicon Dioxide; D006108:Graphite; C057862:cyanoginosin LR
keywords: Enrichment; MALDI-TOF MS analysis; Magnetic graphene; Mesoporous silica; Microcystin-LR