title: Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods.
abstract: In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.
authors.affiliation: Laboratory of Molecular Biology, Fujita Health University Institute of Joint Research, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan. naokiy@fujita-hu.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd, 2-7 Torimi-cho, Nishi-ku, Nagoya, Aichi, 451-0071, Japan.
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd, 2-7 Torimi-cho, Nishi-ku, Nagoya, Aichi, 451-0071, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sato
authors.affiliation: Laboratory of Molecular Biology, Fujita Health University Institute of Joint Research, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Hiramatsu
authors.affiliation: Laboratory of Molecular Biology, Fujita Health University Institute of Joint Research, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamashita
authors.affiliation: Department of Physiology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Mahito
authors.initials: M
authors.lastname: Ohkuma
authors.affiliation: Department of Physiology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Ei-Ichi
authors.initials: E
authors.lastname: Miyachi
authors.affiliation: Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Horiguchi
authors.affiliation: Department of Ophthalmology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Hirano
authors.affiliation: Japanese Center for the Validation of Alternative Methods (JaCVAM), Division of Pharmacology, Biological Safety Research Center, National Institute of Health Sciences, 1-18-1, Kamiyouga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Kojima
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D002460:Cell Line; D003315:Cornea / Q000166:cytology*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis; D004847:Epithelial Cells / Q000166:cytology* / Q000473:pathology; D019573:Epithelium, Corneal / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011817:Rabbits
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21
keywords: CDKN1A; Corneal epithelium; Eye irritation; In vitro model; SIRC cell