title: Can ultrashort-TE (UTE) MRI sequences on a 3-T clinical scanner detect signal directly from collagen protons: freeze-dry and D2 O exchange studies of cortical bone and Achilles tendon specimens.
abstract: Ultrashort-TE (UTE) sequences can obtain signal directly from short-T2 , collagen-rich tissues. It is generally accepted that bound and free water can be detected with UTE techniques, but the ability to detect protons directly on the collagen molecule remains controversial. In this study, we investigated the potential of UTE sequences on a 3-T clinical scanner to detect collagen protons via freeze-drying and D2 O-H2 O exchange studies. Experiments were performed on bovine cortical bone and human Achilles tendon specimens, which were either subject to freeze-drying for over 66 h or D2 O-H2 O exchange for 6 days. Specimens were imaged using two- and three-dimensional UTE with Cones trajectory techniques with a minimum TE of 8 μs at 3 T. UTE images before treatment showed high signal from all specimens with bi-component T2 * behavior. Bovine cortical bone showed a shorter T2 * component of 0.36 ms and a longer T2 * component of 2.30 ms with fractions of 78.2% and 21.8% by volume, respectively. Achilles tendon showed a shorter T2 * component of 1.22 ms and a longer T2 * component of 15.1 ms with fractions of 81.1% and 18.9% by volume, respectively. Imaging after freeze-drying or D2 O-H2 O exchange resulted in either the absence or near-absence of signal. These results indicate that bound and free water are the sole sources of UTE signal in bovine cortical bone and human Achilles tendon samples on a clinical 3-T scanner. Protons on the native collagen molecule are not directly visible when imaged using UTE sequences. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Radiology, University of California, San Diego, CA, USA.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Ma
authors.affiliation: Department of Radiology, University of California, San Diego, CA, USA.|Radiology Service, VA San Diego Healthcare System, San Diego, CA, USA.
authors.forename: Eric Y
authors.initials: EY
authors.lastname: Chang
authors.affiliation: Department of Radiology, University of California, San Diego, CA, USA.
authors.forename: Graeme M
authors.initials: GM
authors.lastname: Bydder
authors.affiliation: Department of Radiology, University of California, San Diego, CA, USA.
authors.forename: Jiang
authors.initials: J
authors.lastname: Du
mesh_terms: D000125:Achilles Tendon / Q000737:chemistry* / Q000000981:diagnostic imaging; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D041961:Deuterium Exchange Measurement / Q000379:methods*; D005269:Femur / Q000737:chemistry*; D005612:Freeze Drying / Q000379:methods*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging / Q000379:methods; D057054:Molecular Imaging / Q000379:methods; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D013048:Specimen Handling / Q000379:methods*
chemical_list: D003094:Collagen
keywords: D2O-H2O exchange; UTE; collagen; freeze-drying