title: Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments.
abstract: We present the results of solid state nuclear magnetic resonance (NMR) experiments on HIV-1 capsid protein (CA) assemblies with three different morphologies, namely wild-type CA (WT-CA) tubes with 35-60 nm diameters, planar sheets formed by the Arg(18)-Leu mutant (R18L-CA), and R18L-CA spheres with 20-100 nm diameters. The experiments are intended to elucidate molecular structural variations that underlie these variations in CA assembly morphology. We find that multidimensional solid state NMR spectra of (15)N,(13)C-labeled CA assemblies are remarkably similar for the three morphologies, with only small differences in (15)N and (13)C chemical shifts, no significant differences in NMR line widths, and few differences in the number of detectable NMR cross-peaks. Thus, the pronounced differences in morphology do not involve major differences in the conformations and identities of structurally ordered protein segments. Instead, morphological variations are attributable to variations in conformational distributions within disordered segments, which do not contribute to the solid state NMR spectra. Variations in solid state NMR signals from certain amino acid side chains are also observed, suggesting differences in the intermolecular dimerization interface between curved and planar CA lattices, as well as possible differences in intramolecular helix-helix packing.
authors.affiliation: From the Laboratory of Chemical Physics, NIDKK, National Institutes of Health, Bethesda, Maryland 20892-0520.
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Lu
authors.affiliation: From the Laboratory of Chemical Physics, NIDKK, National Institutes of Health, Bethesda, Maryland 20892-0520.
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Bayro
authors.affiliation: From the Laboratory of Chemical Physics, NIDKK, National Institutes of Health, Bethesda, Maryland 20892-0520 robertty@mail.nih.gov.
authors.forename: Robert
authors.initials: R
authors.lastname: Tycko
mesh_terms: D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins; D015497:HIV-1 / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D036022:Capsid Proteins
keywords: human immunodeficiency virus (HIV); molecular dynamics; protein assembly; protein structure; solid state NMR