title: The Regulatory Role of Nuclear Factor Kappa B in the Heart of Hereditary Hypertriglyceridemic Rat.
abstract: Activation of nuclear factor-κB (NF-κB) by increased production of reactive oxygen species (ROS) might induce transcription and expression of different antioxidant enzymes and also of nitric oxide synthase (NOS) isoforms. Thus, we aimed at studying the effect of NF-κB inhibition, caused by JSH-23 (4-methyl-N (1)-(3-phenyl-propyl)-benzene-1,2-diamine) injection, on ROS and NO generation in hereditary hypertriglyceridemic (HTG) rats. 12-week-old, male Wistar and HTG rats were treated with JSH-23 (bolus, 10 μmol, i.v.). After one week, blood pressure (BP), superoxide dismutase (SOD) activity, SOD1, endothelial NOS (eNOS), and NF-κB (p65) protein expressions were higher in the heart of HTG rats compared to control rats. On the other hand, NOS activity was decreased. In HTG rats, JSH-23 treatment increased BP and heart conjugated dienes (CD) concentration (measured as the marker of tissue oxidative damage). Concomitantly, SOD activity together with SOD1 expression was decreased, while NOS activity and eNOS protein expression were increased significantly. In conclusion, NF-κB inhibition in HTG rats led to decreased ROS degradation by SOD followed by increased oxidative damage in the heart and BP elevation. In these conditions, increased NO generation may represent rather a counterregulatory mechanism activated by ROS. Nevertheless, this mechanism was not sufficient enough to compensate BP increase in HTG rats.
authors.affiliation: Institute of Normal and Pathological Physiology and Centre of Excellence for Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, 813 71 Bratislava, Slovakia.
authors.forename: Stanislava
authors.initials: S
authors.lastname: Vranková
authors.affiliation: Institute of Normal and Pathological Physiology and Centre of Excellence for Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, 813 71 Bratislava, Slovakia.
authors.forename: Andrej
authors.initials: A
authors.lastname: Barta
authors.affiliation: Institute of Normal and Pathological Physiology and Centre of Excellence for Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, 813 71 Bratislava, Slovakia.
authors.forename: Jana
authors.initials: J
authors.lastname: Klimentová
authors.affiliation: Institute of Normal and Pathological Physiology and Centre of Excellence for Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, 813 71 Bratislava, Slovakia.
authors.forename: Ima
authors.initials: I
authors.lastname: Dovinová
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic; Centre of Cardiovascular Research, 142 20 Prague 4, Czech Republic.
authors.forename: Silvia
authors.initials: S
authors.lastname: Líšková
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic; Centre of Cardiovascular Research, 142 20 Prague 4, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Dobešová
authors.affiliation: Institute of Normal and Pathological Physiology and Centre of Excellence for Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, 813 71 Bratislava, Slovakia.
authors.forename: Oľga
authors.initials: O
authors.lastname: Pecháňová
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic; Centre of Cardiovascular Research, 142 20 Prague 4, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Kuneš
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic; Centre of Cardiovascular Research, 142 20 Prague 4, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Zicha
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000032:analysis; D006352:Heart Ventricles / Q000378:metabolism; D006953:Hyperlipoproteinemia Type IV / Q000473:pathology / Q000662:veterinary; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010655:Phenylenediamines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*
chemical_list: C549066:4-methyl-N1-(3-phenylpropyl)benzene-1,2-diamine; D010655:Phenylenediamines; D017382:Reactive Oxygen Species; D051996:Transcription Factor RelA; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D013482:Superoxide Dismutase; D005978:Glutathione