title: Integrated Study of Globally Expressed microRNAs in IL-1β-stimulated Human Osteoarthritis Chondrocytes and Osteoarthritis Relevant Genes: A Microarray and Bioinformatics Analysis.
abstract: This study was undertaken to identify and characterize the globally expressed microRNAs (miRNAs) involved in interleukin-1β (IL-1β)-induced joint damage and to predict whether miRNAs can regulate the catabolic effects in osteoarthritis (OA) chondrocytes. Out of 1347 miRNAs analyzed by microarrays in IL-1β-stimulated OA chondrocytes, 35 miRNAs were down-regulated, 1 miRNA was up-regulated, and the expression of 1311 miRNAs remained unchanged. Bioinformatics analysis showed the key inflammatory mediators and key molecular pathways are targeted by differentially expressed miRNAs. Novel miRNAs identified could have important diagnostic and therapeutic potentials in the development of novel therapeutic strategies for pain managements in OA.
authors.affiliation: a Department of Medical Biochemistry , College of Medicine, Qassim University , Buraidah , KSA.
authors.forename: Zafar
authors.initials: Z
authors.lastname: Rasheed
authors.affiliation: b Department of Dermatology , College of Medicine, Qassim University , Buraidah , KSA.
authors.forename: Hani A
authors.initials: HA
authors.lastname: Al-Shobaili
authors.affiliation: a Department of Medical Biochemistry , College of Medicine, Qassim University , Buraidah , KSA.
authors.forename: Naila
authors.initials: N
authors.lastname: Rasheed
authors.affiliation: c Department of Pathology , College of Medicine, Qassim University , Buraidah , KSA.
authors.forename: Abdulaziz A M
authors.initials: AA
authors.lastname: Al Salloom
authors.affiliation: d Department of Orthopedics , King Fahd Medical City , Riyadh , KSA.
authors.forename: Osama
authors.initials: O
authors.lastname: Al-Shaya
authors.affiliation: e Stem Cell Unit , Department of Anatomy, College of Medicine, King Saud University , Riyadh , KSA.
authors.forename: Amer
authors.initials: A
authors.lastname: Mahmood
authors.affiliation: e Stem Cell Unit , Department of Anatomy, College of Medicine, King Saud University , Riyadh , KSA.
authors.forename: Nehad M
authors.initials: NM
authors.lastname: Alajez
authors.affiliation: d Department of Orthopedics , King Fahd Medical City , Riyadh , KSA.
authors.forename: Ahmed S S
authors.initials: AS
authors.lastname: Alghamdi
authors.affiliation: c Department of Pathology , College of Medicine, Qassim University , Buraidah , KSA.
authors.forename: El-Sayed E
authors.initials: el-SE
authors.lastname: Mehana
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D019295:Computational Biology; D015870:Gene Expression; D006801:Humans; D053583:Interleukin-1beta / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020370:Osteoarthritis, Knee / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; D053583:Interleukin-1beta; D035683:MicroRNAs
keywords: Osteoarthritis; bioinformatics; chondrocytes; microRNAs; microarray