title: Effect of Roux-en-Y gastric bypass surgery on intestinal Akkermansia muciniphila.
abstract: OBJECTIVE
  To investigated changes in intestinal Akkermansia muciniphila (A. muciniphila) and explored the mechanism underlying the therapeutic effects of Roux-en-Y gastric bypass (RYGB) surgery on type 2 diabetes in diabetic Goto-Kakizaki (GK) rats.
  
  
  METHODS
  Male diabetic GK rats (n = 12) aged 8 wk were randomly assigned to the surgery group (GK-RYGB) or sham surgery group (GK-Sham) (n = 6 per group), and another 6 male Wistar rats aged 8 wk served as controls (WS-Sham). In the surgery group, RYGB surgery was conducted, and a sham operation was performed in both sham groups. Fasting blood glucose (FBG) levels before and after surgery, fasting levels of serum insulin and serum glucagon-like peptide-1 (GLP-1) and levels 30 min after intragastric injection of glucose, and the amount of A. muciniphila in the stool were determined. Insulin and GLP-1 were measured by enzyme-linked immunosorbent assay, and A. muciniphila were detected by fluorescence-based quantitative polymerase chain reaction.
  
  
  RESULTS
  The FBG was improved, and serum GLP-1 and insulin increased significantly (P < 0.05) in the GK-RYGB group after surgery compared to levels before surgery and to levels in the GK-Sham group. Before surgery, the amounts of A. muciniphila in the GK-RYGB and GK-Sham groups were significantly lower than in the WS-Sham group (P < 0.05). After surgery, the amount of A. muciniphila in the GK-RYGB group increased markedly compared to that before surgery and to that in the GK-Sham and WS-Sham groups (P < 0.05). In addition, the A. muciniphila amount was positively related to GLP-1 (r = 0.86, P < 0.05).
  
  
  CONCLUSIONS
  Our results demonstrated RYGB surgery may increase GLP-1 secretion, elevate serum insulin after intragastric injection of glucose, and improve insulin resistance in diabetic GK rats, thereby contributing to a significant reduction in blood glucose. The increased amount of A. muciniphila after RYGB surgery may be related to elevated GLP-1 secretion.
authors.affiliation: Ming Yan, Mao-Min Song, Ri-Xing Bai, Shi Cheng, Wen-Mao Yan, Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Ming Yan, Mao-Min Song, Ri-Xing Bai, Shi Cheng, Wen-Mao Yan, Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Mao-Min
authors.initials: MM
authors.lastname: Song
authors.affiliation: Ming Yan, Mao-Min Song, Ri-Xing Bai, Shi Cheng, Wen-Mao Yan, Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Ri-Xing
authors.initials: RX
authors.lastname: Bai
authors.affiliation: Ming Yan, Mao-Min Song, Ri-Xing Bai, Shi Cheng, Wen-Mao Yan, Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Ming Yan, Mao-Min Song, Ri-Xing Bai, Shi Cheng, Wen-Mao Yan, Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Wen-Mao
authors.initials: WM
authors.lastname: Yan
keywords: Akkermansia mucinipilia; Glucagon-like peptide-1; Glucose-dependent insulinotropic peptide; Roux-en-Y gastric bypass surgery; Type 2 diabetes