title: Neuronal cell lines as model dorsal root ganglion neurons: A transcriptomic comparison.
abstract: BACKGROUND
  Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with those expressed in intact peripheral neurons is lacking.
  
  
  RESULTS
  In this study, we examined the expression of RNA transcripts in the human neuroblastoma-derived cell line, SH-SY5Y, and two dorsal root ganglion hybridoma cell lines, F-11 and ND7/23, using Illumina next-generation sequencing, and compared the results with native whole murine dorsal root ganglions. The gene expression profiles of these three cell lines did not resemble any specific defined dorsal root ganglion subclass. The cell lines lacked many markers for nociceptive sensory neurons, such as the Transient receptor potential V1 gene, but expressed markers for both myelinated and unmyelinated neurons. Global gene ontology analysis on whole dorsal root ganglions and cell lines showed similar enrichment of biological process terms across all samples.
  
  
  CONCLUSIONS
  This paper provides insights into the receptor repertoire expressed in common dorsal root ganglion neuron-derived cell lines compared with whole murine dorsal root ganglions, and illustrates the limits and potentials of these cell lines as tools for neuropharmacological exploration.
authors.affiliation: Centre for Pain Research, Institute for Molecular Bioscience, University of Queensland, Queensland, Australia Pharmacy Australia Centre of Excellence, University of Queensland, Queensland, Australia.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Yin
authors.affiliation: Centre for Pain Research, Institute for Molecular Bioscience, University of Queensland, Queensland, Australia.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Baillie
authors.affiliation: Centre for Pain Research, Institute for Molecular Bioscience, University of Queensland, Queensland, Australia Pharmacy Australia Centre of Excellence, University of Queensland, Queensland, Australia i.vetter@uq.edu.au.
authors.forename: Irina
authors.initials: I
authors.lastname: Vetter
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D063990:Gene Ontology; D057809:HEK293 Cells; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010121:Oxytocin / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics*; D050051:Transient Receptor Potential Channels / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D007473:Ion Channels; D043562:Receptors, G-Protein-Coupled; D050051:Transient Receptor Potential Channels; D014667:Vasopressins; D010121:Oxytocin; D002118:Calcium
keywords: Dorsal root ganglions; F-11; ND7/23; SH-SY5Y; molecular expression; transcriptomic analysis