title: Enhanced autophagy signaling in diabetic rats with ischemia-induced seizures.
abstract: Seizures are among the most common neurological sequelae of stroke, and ischemic insult in diabetes notably increases the incidence of seizures. Recent studies indicated that autophagy influences the outcome of stroke and involved in epileptogenesis. However, the association of autophagy and post-ischemic seizures in diabetes remains unclear. The present study aimed to reveal the involvement of autophagy in the seizures following cerebral ischemia in diabetes. Diabetes was induced in adult male Wistar rats by intraperitoneal injection of streptozotocin (STZ). The diabetic rats were subjected to transient forebrain ischemia. The neuronal damage was assessed using hematoxylin-eosin staining. Western blotting and immunohistochemistry were performed to investigate the alteration of autophagy marker microtubule-associated protein light chain 1B (LC3B). The results showed that all diabetic animals developed seizures after ischemia. However, no apparent cell death was observed in the hippocampus of seizure rats 12h after the insult. The expression of LC3B was significantly enhanced in naïve animals after ischemia and was further increased in diabetic animals after ischemia. Immunofluorescence double-labeling study indicated that LC3B was mainly increased in neurons. Our study demonstrated, for the first time, that autophagy activity is significantly increased in diabetic animals with ischemia-induced seizures. Further studies are needed to explore the role of autophagy in seizure generation after ischemia in diabetic conditions.
authors.affiliation: GHM Institute of CNS Regeneration (GHMICR), Jinan University, Guangzhou, China.
authors.forename: Luoxing
authors.initials: L
authors.lastname: Xia
authors.affiliation: Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Lei
authors.affiliation: GHM Institute of CNS Regeneration (GHMICR), Jinan University, Guangzhou, China.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Dave
authors.initials: D
authors.lastname: Guo
authors.affiliation: Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Henry
authors.initials: H
authors.lastname: Su
authors.affiliation: GHM Institute of CNS Regeneration (GHMICR), Jinan University, Guangzhou, China; Department of Anatomy, Jinan University School of Medicine, Guangzhou, China. Electronic address: tyiwen@jnu.edu.cn.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA. Electronic address: zcxu@iupui.edu.
authors.forename: Zao C
authors.initials: ZC
authors.lastname: Xu
mesh_terms: D000818:Animals; D001343:Autophagy; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism*; D048909:Diabetes Complications / Q000378:metabolism*; D004195:Disease Models, Animal; D006624:Hippocampus / Q000473:pathology / Q000502:physiology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000209:etiology / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D008869:Microtubule-Associated Proteins; C526109:Anxa3 protein, rat
keywords: Epilepsy; Hippocampus; Hyperglycemia; LC3; Stroke