title: Anaerobic digestion in mesophilic and room temperature conditions: Digestion performance and soil-borne pathogen survival.
abstract: Tomato plant waste (TPW) was used as the feedstock of a batch anaerobic reactor to evaluate the effect of anaerobic digestion on Ralstonia solanacearum and Phytophthora capsici survival. Batch experiments were carried out for TS (total solid) concentrations of 2%, 4% and 6% respectively, at mesophilic (37±1°C) and room (20-25°C) temperatures. Results showed that higher digestion performance was achieved under mesophilic digestion temperature and lower TS concentration conditions. The biogas production ranged from 71 to 416L/kg VS (volatile solids). The inactivation of anaerobic digestion tended to increase as digestion performance improved. The maximum log copies reduction of R. solanacearum and P. capsici detected by quantitative PCR (polymerase chain reaction) were 3.80 and 4.08 respectively in reactors with 4% TS concentration at mesophilic temperatures. However, both in mesophilic and room temperature conditions, the lowest reduction of R. solanacearum was found in the reactors with 6% TS concentration, which possessed the highest VFA (volatile fatty acid) concentration. These findings indicated that simple accumulation of VFAs failed to restrain R. solanacearum effectively, although the VFAs were considered poisonous. P. capsici was nearly completely dead under all conditions. Based on the digestion performance and the pathogen survival rate, a model was established to evaluate the digestate biosafety.
authors.affiliation: Laboratory of Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Le
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Jian
authors.affiliation: Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Bi
authors.affiliation: Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; East China Scientific Observing and Experimental Station of Development and Utilization of Rural Renewable Energy, Ministry of Agriculture, Nanjing 210014, China.
authors.forename: Zhizhou
authors.initials: Z
authors.lastname: Chang
authors.affiliation: Laboratory of Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Laboratory of Environmental Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; East China Scientific Observing and Experimental Station of Development and Utilization of Rural Renewable Energy, Ministry of Agriculture, Nanjing 210014, China. Electronic address: yexiaomei610@126.com.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Ye
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels; D019149:Bioreactors; D005232:Fatty Acids, Volatile / Q000378:metabolism; D010838:Phytophthora / Q000502:physiology*; D043367:Ralstonia / Q000502:physiology*; D012037:Refuse Disposal / Q000379:methods*; D012987:Soil / Q000737:chemistry / Q000469:parasitology*; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D012987:Soil
keywords: Anaerobic digestion; Digestion performance; Fruit and vegetable wastes; Pathogen inactivation