title: Indole-3-acetic acid-induced oxidative burst and an increase in cytosolic calcium ion concentration in rice suspension culture.
abstract: Indole-3-acetic acid (IAA) is the major natural auxin involved in the regulation of a variety of growth and developmental processes such as division, elongation, and polarity determination in growing plant cells. It has been shown that dividing and/or elongating plant cells accompanies the generation of reactive oxygen species (ROS) and a number of reports have suggested that hormonal actions can be mediated by ROS through ROS-mediated opening of ion channels. Here, we surveyed the link between the action of IAA, oxidative burst, and calcium channel activation in a transgenic cells of rice expressing aequorin in the cytosol. Application of IAA to the cells induced a rapid and transient generation of superoxide which was followed by a transient increase in cytosolic Ca(2+) concentration ([Ca(2+)]c). The IAA-induced [Ca(2+)]c elevation was inhibited by Ca(2+) channel blockers and a Ca(2+) chelator. Furthermore, ROS scavengers effectively blocked the action of IAA on [Ca(2+)]c elevation.
authors.affiliation: a Laboratory of Chemical Biology and Bioengineering, Graduate School and Faculty of Environmental Engineering , The University of Kitakyushu , Kitakyushu , Japan.
authors.forename: Hieu T H
authors.initials: HT
authors.lastname: Nguyen
authors.affiliation: b Agricultural & Veterinary Research Laboratories , Meiji Seika Pharma Co., Ltd. , Yokohama , Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Umemura
authors.affiliation: a Laboratory of Chemical Biology and Bioengineering, Graduate School and Faculty of Environmental Engineering , The University of Kitakyushu , Kitakyushu , Japan.|c University of Florence LINV Kitakyushu Research Center (LINV@Kitakyushu) , Kitakyushu , Japan.|d Univ. Paris-Diderot, Sorbonne Paris Cité, Paris 7 Interdisciplinary Energy Research Institute (PIERI) , Paris , France.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kawano
mesh_terms: D000331:Aequorin / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D065096:Calcium Chelating Agents / Q000494:pharmacology; D002413:Cations, Divalent; D018929:Cell Culture Techniques; D004533:Egtazic Acid / Q000494:pharmacology; D015870:Gene Expression; D017930:Genes, Reporter; D007210:Indoleacetic Acids / Q000494:pharmacology*; D017136:Ion Transport; D008163:Luminescent Measurements; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D059828:Plant Cells / Q000187:drug effects* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects; D015398:Signal Transduction; D014700:Verapamil / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D065096:Calcium Chelating Agents; D002413:Cations, Divalent; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D000331:Aequorin; D004533:Egtazic Acid; C030737:indoleacetic acid; D014700:Verapamil; D002118:Calcium
keywords: Oryza sativa L; ROS; aequorin; auxin; calcium