title: Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation.
abstract: The adult zebrafish has been used to model the electrocardiogram (ECG) for human cardiovascular studies. Nonetheless huge variations are observed among studies probably because of the lack of a reliable and reproducible recording method. In our study, an adult zebrafish surface ECG recording technique was improved using a multi-electrode method and by pre-opening the pericardial sac. A convenient ECG data analysis method without wavelet transform was also established. Intraperitoneal injection of KCl in zebrafish induced an arrhythmia similar to that of humans, and the arrhythmia was partially rescued by calcium gluconate. Amputation and cryoinjury of the zebrafish heart induced ST segment depression and affected QRS duration after injury. Only cryoinjury decelerated the heart rate. Different changes were also observed in the QT interval during heart regeneration in these two injury models. We also characterized the electrocardiophysiology of breakdance zebrafish mutant with a prolonged QT interval, that has not been well described in previous studies. Our study provided a reliable and reproducible means to record zebrafish ECG and analyse data. The detailed characterization of the cardiac electrophysiology of zebrafish and its mutant revealed that the potential of the zebrafish in modeling the human cardiovascular system exceeds expectations.
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
authors.forename: Chi Chi
authors.initials: CC
authors.lastname: Liu
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China.
authors.forename: Yun Wah
authors.initials: YW
authors.lastname: Lam
authors.affiliation: Cardiology Division, Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Chung Wah
authors.initials: CW
authors.lastname: Siu
authors.affiliation: Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
authors.forename: Shuk Han
authors.initials: SH
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology; D002125:Calcium Gluconate / Q000008:administration & dosage / Q000627:therapeutic use; D004195:Disease Models, Animal; D004562:Electrocardiography / Q000379:methods*; D006321:Heart / Q000502:physiology*; D006339:Heart Rate; D007274:Injections, Intraperitoneal; D009154:Mutation; D011189:Potassium Chloride / Q000009:adverse effects; D015027:Zebrafish / Q000235:genetics / Q000502:physiology*
chemical_list: D011189:Potassium Chloride; D002125:Calcium Gluconate