title: Pharmacokinetics and Tissue Distribution of Folate-Decorated Human Serum Albumin Loaded With Nano-Hydroxycamptothecin for Tumor Targeting.
abstract: The goal of this work is to develop the method of preparing folate (FA)-decorated human serum albumin (HSA) loaded with nano-hydroxycamptothecin (nHCPT) nanoparticles (NPs) (FA-HSA-nHCPT-NPs) and to explore its antitumor activity in vivo and in vitro. FA-HSA-nHCPT-NPs were obtained by preparing nHCPT by a high-pressure homogenization technique followed with an anti-solvent method. The drug-loading efficiency of the FA-HSA-nHCPT-NPs was 7.8%. We adopted the human breast cancer cells (FA receptor-expressing MCF-7 cells) and BALB/c mice inoculated with human MCF-7 cells to determine the antitumor activity of FA-HSA-nHCPT-NPs in vitro and in vivo, respectively. The antitumor activity of FA-HSA-nHCPT-NPs was stronger than that of the raw HCPT in both conditions. Tissue distribution analysis showed that the FA-HSA-nHCPT-NPs carried more HCPT to tumors than the raw HCPT. The tumor inhibitory rate of FA-HSA-nHCPT-NPs was much higher compared with the raw HCPT. Th7us, the FA-HSA-nHCPT-NPs could serve as a viable delivery system with an obvious target effect on tumor.
authors.affiliation: Collaborative Innovation Center of Yangtze River Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou City, China; Key Laboratory of Forest Plant Ecology (Northeast Forestry University), Ministry of Education, Harbin, Heilongjiang, China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center of Yangtze River Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou City, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.affiliation: Key Laboratory of Forest Plant Ecology (Northeast Forestry University), Ministry of Education, Harbin, Heilongjiang, China.
authors.forename: Baihe
authors.initials: B
authors.lastname: Sun
authors.affiliation: Collaborative Innovation Center of Yangtze River Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou City, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Collaborative Innovation Center of Yangtze River Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou City, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Collaborative Innovation Center of Yangtze River Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou City, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Collaborative Innovation Center of Yangtze River Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou City, China; Key Laboratory of Forest Plant Ecology (Northeast Forestry University), Ministry of Education, Harbin, Heilongjiang, China. Electronic address: liqy@zjut.edu.cn.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000008:administration & dosage / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage / Q000378:metabolism*; D000075462:Serum Albumin, Human / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D004337:Drug Carriers; C527042:hydroxycamptothecinum; D005492:Folic Acid; D002166:Camptothecin; D000075462:Serum Albumin, Human
keywords: folate-decorated human serum albumin; hydroxycamptothecin; targeted delivery system; tissue distribution