title: In Vivo Evaluation of Anti Diabetic, Hypolipidemic, Antioxidative Activities of Saudi Date Seed Extract on Streptozotocin Induced Diabetic Rats.
abstract: BACKGROUND
  Phoenix dactylifera (date palm) is major fruit of gulf region. In folk medicine; dates have been traditionally use. The date seed is used as hypoglycaemic, expectorant, tonic, aphrodisiac, antidiarrheic and mouth hygiene.
  
  
  OBJECTIVE
  This study intended to evaluate the anti-diabetic, hypolipidaemic and antioxidative activities of date seed extract in diabetes-induced rats.
  
  
  METHODS
  Total of seven groups of rats, consisting of control rats and streptozotocin induced diabetic rats treated with aqueous seed extract in concentration of 100g/L in dosage of 10ml/day/rat. To evaluate the anti-diabetic property, glucose and weight was analysed weekly and at the end of eight week all rats were sacrificed. To evaluate the hypolipidaemic and antioxidative activities, serum cholesterol, triglyceride, malondialdehyde, superoxide dismutase, 8-hydroxy-2'-deoxyguanosine were estimated. Liver enzymes and kidney function tests were performed. Moreover to verify the glycaemic effect; glycated haemoglobin and serum insulin was performed.
  
  
  RESULTS
  Aqueous seed extract in concentration of 100 gm/L in dosage of 10ml/day/rat brings a significant reduction of blood glucose levels in diabetic rats in comparison of control rats. There were significant differences in the investigated clinical chemistry and oxidative stress parameters between control and diabetic rats with both seed extract of Ajwa and Sukkari dates.
  
  
  CONCLUSIONS
  Present study verifies the antidiabetic property, of aqueous seed extracts of two different varieties of dates namely Ajwa and Sukkari of Kingdom of Saudi on streptozotocin induced Diabetic rats. Prolong treatments with the extract restores the function of liver and kidney and balance the oxidative stress condition in diabetic treated rats.
authors.affiliation: Lecturer, Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University , Kingdom of Saudi Arabia .
authors.forename: Marghoob
authors.initials: M
authors.lastname: Hasan
authors.affiliation: Associate Professor, Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University , Kingdom of Saudi Arabia .
authors.forename: Abdelmarouf
authors.initials: A
authors.lastname: Mohieldein
keywords: Ajwa; Anti diabetic; Diabetes; Oxidative stress; Sukkari