title: Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization.
abstract: The Dsb protein family is responsible for introducing disulfide bonds into nascent proteins in prokaryotes, stabilizing the structure of many proteins. Helicobacter pylori HP0231 is a Dsb-like protein, shown to catalyze disulfide bond formation and to participate in redox homeostasis. Notably, many H. pylori virulence factors are stabilized by the formation of disulfide bonds. By employing H. pylori HP0231 deficient strains we analyzed the effect of lack of this bacterial protein on the functionality of virulence factors containing putative disulfide bonds. The lack of H. pylori HP0231 impaired CagA translocation into gastric epithelial cells and reduced VacA-induced cellular vacuolation. Moreover, H. pylori HP0231 deficient bacteria were not able to colonize the gastric mucosa of mice, probably due to compromised motility. Together, our data demonstrate an essential function for H. pylori HP0231 in gastric colonization and proper function of bacterial virulence factors related to gastric pathology.
authors.affiliation: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Anderl
authors.affiliation: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Kruse
authors.affiliation: Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig-Maximilians-Universität, Munich, Germany.|German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Schindele
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Elżbieta Katarzyna
authors.initials: EK
authors.lastname: Jagusztyn-Krynicka
authors.affiliation: Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig-Maximilians-Universität, Munich, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fischer
authors.affiliation: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.|German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Gerhard
authors.affiliation: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.|German Centre for Infection Research (DZIF), partner site Munich, Munich, Germany.
authors.forename: Raquel
authors.identifier: 0000-0002-4602-4927
authors.initials: R
authors.lastname: Mejías-Luque
mesh_terms: D000942:Antigens, Bacterial / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002460:Cell Line; D016480:Helicobacter pylori / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D021381:Protein Transport; D013270:Stomach / Q000382:microbiology*; D014617:Vacuoles / Q000382:microbiology; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori