title: Graphene oxide decorated electrospun gelatin nanofibers: Fabrication, properties and applications.
abstract: Gelatin nanofiber fabricated by electrospinning process is found to mimic the complex structural and functional properties of natural extracellular matrix for tissue regeneration. In order to improve the physico-chemical and biological properties of the nanofibers, graphene oxide is incorporated in the gelatin to form graphene oxide decorated gelatin nanofibers. The current research effort is focussed on the fabrication and evaluation of physico-chemical and biological properties of graphene oxide-gelatin composite nanofibers. The presence of graphene oxide in the nanofibers was established by transmission electron microscopy (TEM). We report the effect of incorporation of graphene oxide on the mechanical, thermal and biological performance of the gelatin nanofibers. The tensile strength of gelatin nanofibers was increased from 8.29±0.53MPa to 21±2.03MPa after the incorporation of GO. In order to improve the water resistance of nanofibers, natural based cross-linking agent, namely, dextran aldehyde was employed. The cross-linked composite nanofibers showed further increase in the tensile strength up to 56.4±2.03MPa. Graphene oxide incorporated gelatin nanofibers are evaluated for bacterial activity against gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacteria and cyto compatibility using mouse fibroblast cells (L-929 cells). The results indicate that the graphene oxide incorporated gelatin nanofibers do not prevent bacterial growth, nevertheless support the L-929 cell adhesion and proliferation.
authors.affiliation: Department of Chemistry, Indian Institute of Space Science and Technology, Valiamala, Thiruvananthapuram, Kerala 695 547, India.
authors.forename: K
authors.initials: K
authors.lastname: Jalaja
authors.affiliation: Indian Institute of Science Education and Research Bhopal, Bhauri, Madhya Pradesh 462066, India.
authors.forename: V S
authors.initials: VS
authors.lastname: Sreehari
authors.affiliation: Tissue culture laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram, Kerala 695 012, India.
authors.forename: P R Anil
authors.initials: PRA
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, Indian Institute of Space Science and Technology, Valiamala, Thiruvananthapuram, Kerala 695 547, India. Electronic address: nirmala@iist.ac.in.
authors.forename: R James
authors.initials: RJ
authors.lastname: Nirmala
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D004926:Escherichia coli / Q000254:growth & development; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000254:growth & development; D013718:Tensile Strength
chemical_list: D006108:Graphite; D005780:Gelatin
keywords: Antibacterial; Cell adhesion; Gelatin; Graphene oxide; Nanofibers