title: Expression profile of key immune-related genes in Penaeus monodon juveniles after oral administration of recombinant envelope protein VP28 of white spot syndrome virus.
abstract: White spot syndrome virus (WSSV) is the most catastrophic pathogen the shrimp industry has ever encountered. VP28, the abundant envelope protein of WSSV was expressed in bacteria, the purified protein administered orally to Penaeus monodon juveniles and its immune modulatory effects examined. The results indicated significant up-regulation of caspase, penaeidin, crustin, astakine, syntenin, PmRACK, Rab7, STAT and C-type lectin in animals orally administered with this antigen. This revealed the immune modulations in shrimps followed by oral administration of rVP28P which resulted in the reduced transcription of viral gene vp28 and delay in mortality after WSSV challenge. The study suggests the potential of rVP28P to elicit a non-specific immune stimulation in shrimps.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India. Electronic address: anci.thomas@gmail.com.
authors.forename: Ancy
authors.initials: A
authors.lastname: Thomas
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin, India. Electronic address: cusatmarine@gmail.com.
authors.forename: Naduvilamuriparampu Saidumuhammed
authors.initials: NS
authors.lastname: Sudheer
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049, Bodø, Norway. Electronic address: kiron.viswanath@hibo.no.
authors.forename: Viswanath
authors.initials: V
authors.lastname: Kiron
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin, India. Electronic address: isbsingh@gmail.com.
authors.forename: Issac S
authors.initials: IS
authors.lastname: Bright Singh
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India. Electronic address: rbn@annauniv.edu.
authors.forename: Rangarajan Badri
authors.initials: RB
authors.lastname: Narayanan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D020869:Gene Expression Profiling; D007155:Immunologic Factors / Q000096:biosynthesis* / Q000235:genetics; D033561:Penaeidae / Q000276:immunology / Q000821:virology*; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D007155:Immunologic Factors; C548473:VP28 protein, white spot syndrome virus; D014759:Viral Envelope Proteins