title: Electrochemical non-enzyme sensor for detecting clenbuterol (CLB) based on MoS2-Au-PEI-hemin layered nanocomposites.
abstract: Clenbuterol (CLB), a kind of β2-adrenergic agonists, could disrupt cardiovascular and central nervous system. In this work, a new electrochemical non-enzyme sensor for detecting Clenbuterol (CLB) was fabricated based on MoS2-Au-PEI-hemin layered nanocomposites. The two-dimensional (2-D) MoS2nanosheets were first in-situ assembled with Au nanoparticles, and polyethylenimine (PEI), then hemin molecules were immobilized onto the MoS2-Au-PEI film-modified glassy carbon electrode (GCE) via amide bond. Scanning electron microscopy (SEM) and Zeta potential measurements were employed to characterize the MoS2-based nanomaterials. Cyclic voltammetry (CV) was used to investigate electrochemical activity of the immobilized hemin on the modified electrode. Upon the optimum conditions, the proposed electrochemical sensor showed an excellent response for CLB including a wide linear ranging from 10ng/mL to 2μg/mL and a detection limit (LOD) of 1.92ng/mL CLB (S/N=3) with favorable reproducibility and stability. Furthermore, this presented method could be feasible for determining CLB in the real pork samples.
authors.affiliation: The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.
authors.forename: Chusen
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal University, Quzhou 362000, Fujian Province, China. Electronic address: yangdp@qztc.edu.cn.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Yang
authors.affiliation: The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China. Electronic address: nqjia@shnu.edu.cn.
authors.forename: Nengqing
authors.initials: N
authors.lastname: Jia
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D002976:Clenbuterol / Q000032:analysis*; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D006046:Gold / Q000737:chemistry; D006427:Hemin / Q000737:chemistry*; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000737:chemistry*; D000069466:Red Meat / Q000032:analysis*; D013552:Swine
chemical_list: D000318:Adrenergic beta-Agonists; D004220:Disulfides; D006427:Hemin; D006046:Gold; D008982:Molybdenum; D011094:Polyethyleneimine; D002976:Clenbuterol; C082964:molybdenum disulfide
keywords: 2-D nanomaterial; Clenbuterol (CLB); Electrochemical non-enzyme sensor; MoS(2)-Au-PEI-hemin nanocomposites