title: Dysregulation of MicroRNA-543 expression in colorectal cancer promotes tumor migration and invasion.
abstract: MicroRNAs (miRNAs) have been proven to act as key post-transcriptional regulators of gene expression, involved in the genesis and development of multiple tumor types. However, the expression and roles of miR-543 in colorectal cancer (CRC) remain unknown. Here, we found that miR-543 was constitutively up-regulated in CRC tissues and its over-expression promoted tumor cell migration and invasion. Moreover, we identified PTEN as a direct target of miR-543 using subsequent software analysis and a dual-luciferase reporter assay. In conclusion, data presented here demonstrate that miR-543 acts as a tumor promoter and plays a vital role in CRC metastasis. These findings confirm that miR-543 may potentially serve as a novel therapeutic target for treating CRC. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of General Surgery, Gastrointestinal Surgery, The First Hospital, China Medical University, Shenyang, Liaoning Province, China.|Department of General Surgery, Colorectal & Anal Surgery,, The People's Hospital of Liaoning Province, Shenyang, Liaoning Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Gastrointestinal Surgery, The First Hospital, China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Gastrointestinal Surgery, The First Hospital, China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Dong
authors.affiliation: Department of General Surgery, Gastrointestinal Surgery, The First Hospital, China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Sheng
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D003106:Colon / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D012007:Rectum / Q000473:pathology*; D015854:Up-Regulation
chemical_list: C577592:MIRN543 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: PTEN; colorectal cancer; invasion; miR-543; migration