title: Involvement of Antibiotic Efflux Machinery in Glutathione-Mediated Decreased Ciprofloxacin Activity in Escherichia coli.
abstract: We have analyzed the contribution of different efflux components to glutathione-mediated abrogation of ciprofloxacin's activity in Escherichia coli and the underlying potential mechanism(s) behind this phenomenon. The results indicated that glutathione increased the total active efflux, thereby partially contributing to glutathione-mediated neutralization of ciprofloxacin's antibacterial action in E. coli However, the role of glutathione-mediated increased efflux becomes evident in the absence of a functional TolC-AcrAB efflux pump.
authors.affiliation: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden manishg@barc.gov.in.
authors.forename: Manish
authors.initials: M
authors.lastname: Goswami
authors.affiliation: Bio-Organic Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Subramanian
authors.affiliation: The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden.
authors.forename: Ranjeet
authors.initials: R
authors.lastname: Kumar
authors.affiliation: The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden.
authors.forename: Jana
authors.initials: J
authors.lastname: Jass
authors.affiliation: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
authors.forename: Narendra
authors.initials: N
authors.lastname: Jawali
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics; D005978:Glutathione / Q000378:metabolism; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D002939:Ciprofloxacin; D005978:Glutathione