title: Antimicrobial coatings based on zinc oxide and orange oil for improved bioactive wound dressings and other applications.
abstract: This work presents a novel nano-modified coating for wound dressings and other medical devices with anti-infective properties, based on functionalized zinc oxide nanostructures and orange oil (ZnO@OO). The obtained nanosurfaces were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), selected area electron diffraction (SAED), differential thermal analysis-thermogravimetry (DTA-TG), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy. The obtained nanocomposite coatings exhibited an antimicrobial activity superior to bare ZnO nanoparticles (NPs) and to the control antibiotic against Staphylococcus aureus and Escherichia coli, as revealed by the lower minimal inhibitory concentration values. For the quantitative measurement of biofilm-embedded microbial cells, a culture-based, viable cell count method was used. The coated wound dressings proved to be more resistant to S. aureus microbial colonization and biofilm formation compared to the uncoated controls. These results, correlated with the good in vivo biodistribution open new directions for the design of nanostructured bioactive coating and surfaces, which can find applications in the medical field, for obtaining improved bioactive wound dressings and prosthetic devices, but also in food packaging and cosmetic industry.
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Romania; grumezescu@yahoo.com.
authors.forename: Marius
authors.initials: M
authors.lastname: Rădulescu
authors.forename: Ecaterina
authors.initials: E
authors.lastname: Andronescu
authors.forename: Andreea
authors.initials: A
authors.lastname: Cirja
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Holban
authors.forename: LaurenŢiu
authors.initials: L
authors.lastname: Mogoantă
authors.forename: Tudor Adrian
authors.initials: TA
authors.lastname: Bălşeanu
authors.forename: Bogdan
authors.initials: B
authors.lastname: Cătălin
authors.forename: Tiberiu Paul
authors.initials: TP
authors.lastname: Neagu
authors.forename: Ioan
authors.initials: I
authors.lastname: Lascăr
authors.forename: Denisa Alexandra
authors.initials: DA
authors.lastname: Florea
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.forename: Bianca
authors.initials: B
authors.lastname: Ciubuca
authors.forename: Veronica
authors.initials: V
authors.lastname: Lazăr
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bolocan
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001458:Bandages; D020099:Coated Materials, Biocompatible / Q000494:pharmacology / Q000627:therapeutic use*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D010938:Plant Oils / Q000494:pharmacology / Q000627:therapeutic use*; D013211:Staphylococcus aureus / Q000187:drug effects; D013818:Thermogravimetry; D014947:Wounds and Injuries / Q000188:drug therapy*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D010938:Plant Oils; C087245:orange oil; D015034:Zinc Oxide