title: Identification of genetic variants in TNF receptor 2 which are associated with the development of cervical carcinoma.
abstract: Cervical cancer is one of the most common malignancies among women in India. Beside HPV, other factors present in host also put their role in the progression of cervical tumerogenesis. In present study, we screened 300 subjects to identify variations in TNFR2 gene by PCR-dHPLC method followed by direct sequencing. We identified six known and four novel variations in six different exons of TNFR2 gene. Out of these identified variations, five known variations were found to be significantly associated with the risk of cervical cancer (p < 0.0001). On construction of haplotypes, one haplotype (TTGCC) was emerged as a major protective type while two (CAAGC + CTGCC) were revealed as major risk haplotypes. In conclusion, postmenopausal women having CAAGC + CTGCC haplotypes in TNFR2 gene along with HPV infection and tobacco consumption may lead to the development of cervical cancer.
authors.affiliation: a Division of Molecular Genetics and Biochemistry , ICPO, ICMR , Noida , India ;|b Department of Biochemistry , University of Delhi South Campus (UDSC) , New Delhi , India.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Singhal
authors.affiliation: a Division of Molecular Genetics and Biochemistry , ICPO, ICMR , Noida , India ;
authors.forename: Upma
authors.initials: U
authors.lastname: Sharma
authors.affiliation: a Division of Molecular Genetics and Biochemistry , ICPO, ICMR , Noida , India ;
authors.forename: Showket
authors.initials: S
authors.lastname: Hussain
authors.affiliation: b Department of Biochemistry , University of Delhi South Campus (UDSC) , New Delhi , India.
authors.forename: Alo
authors.initials: A
authors.lastname: Nag
authors.affiliation: a Division of Molecular Genetics and Biochemistry , ICPO, ICMR , Noida , India ;
authors.forename: Mausumi
authors.initials: M
authors.lastname: Bharadwaj
mesh_terms: D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007194:India; D027383:Papillomaviridae / Q000472:pathogenicity; D017698:Postmenopause; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000235:genetics*; D064424:Tobacco Use / Q000009:adverse effects; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000235:genetics*
chemical_list: D047889:Receptors, Tumor Necrosis Factor, Type II
keywords: Cervical cancer; HPV; TNFR2; dHPLC; polymorphism