title: Effects of γ-Irradiation on the Molecular Structures and Functions of Human Serum Albumin.
abstract: In this paper, we use spectroscopic methods (fluorescence spectroscopy, UV absorption spectroscopy, and circular dichroism (CD) spectroscopy) to elucidate the effects of reactive oxygen species generated by γ-irradiation on the molecular properties of human serum albumin (HSA). The results of fluorescence spectroscopy indicated that oxidation by γ-irradiation can lead to conformational changes of HSA. Data of CD spectra suggested that with the increase of radiation dose the percentage of α-helix in HSA has decreased. The determination of protein hydrophobicity showed that the effective hydrophobicity of HSA decreased up to 62% compared to the native HSA solution due to the exposure to the γ-irradiation. Furthermore, small changes in the esterase-like activity of HSA were introduced because of oxidation. The content of bityrosine increased markedly, suggesting that the oxidized HSA was aggregated. Moreover, there was no obvious change in the molecular properties of HSA with low γ-irradiation dose. Changes happened when the irradiation dose exceeded 200 Gy.
authors.affiliation: School of Environmental Science and Engineering, China -America CRC for Environment & Health, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Environmental Science and Engineering, China -America CRC for Environment & Health, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.affiliation: Library of Beijing Institute of Technology, Beijing, 100081, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, China -America CRC for Environment & Health, Shandong University, Jinan, 250100, People's Republic of China.|Institute of Agricultural Quality Standards and Testing Technology, Shandong Academy of Agricultural Science, Jinan, 250100, People's Republic of China.
authors.forename: Changying
authors.initials: C
authors.lastname: Guo
authors.affiliation: School of Environmental Science and Engineering, China -America CRC for Environment & Health, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Yu
authors.affiliation: School of Environmental Science and Engineering, China -America CRC for Environment & Health, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D002942:Circular Dichroism; D004307:Dose-Response Relationship, Radiation; D004950:Esterases / Q000737:chemistry; D005720:Gamma Rays; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D010084:Oxidation-Reduction; D066329:Protein Aggregates; D017433:Protein Structure, Secondary; D017382:Reactive Oxygen Species / Q000819:agonists* / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D066329:Protein Aggregates; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D014443:Tyrosine; C007543:dityrosine; D004950:Esterases
keywords: Bityrosin; Esterase-Like Activity; Human Serum Albumin; Hydrophobicity; Reactive Oxygen Species; γ-Irradiation