title: [Establishing a 29 Y-STR Loci Multiplex PCR System].
abstract: OBJECTIVE
  To establish a 29 Y-STR loci multiplex PCR system for investigating the genetic polymorphisms and to assess its application value in forensic science.
  
  
  METHODS
  A multiplex PCR system was established using a five color fluorescence labeling 29 Y-STR loci (DYS456, DYS389 I , DYS437, DYS447, DYS389 11, DYS438, DYS522, DYS460, DYS458, DYS622, DYS390, DYS392, DYS448, DYS449, DYS391, Y-GA TA-H4, DYS388, DYS19, DYS385a/b, DYS527a/b, DYS393, DYS459a/b, DYS635, DYS439, DYS570 and DYS627) for multiple amplification and capillary electrophoresis. And its applicability was validated with genetic polymorphism data of 29 Y-STR of unrelated 2,000 male samples in Shandong Han population.
  
  
  RESULTS
  A total of 1,981 different haplotypes of 2,000 individuals showed genotype diver- sity between 0.370 0 and 0.965 4. The system provided stable and accurate typing with high sensitivity of 0.05 ng. It satisfied the needs of variety of routine biological samples.
  
  
  CONCLUSIONS
  The 29 Y-STR loci multiplex PCR system could be applied for actual cases and establishment of Y-STR database. In addition, it has great significance in forensic science practices and related research.
authors.forename: Xin-jie
authors.initials: XJ
authors.lastname: Wang
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005006:Ethnicity / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods; D044707:Forensic Sciences; D005828:Genetics, Population / Q000379:methods; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results
chemical_list: D004247:DNA