title: Mucosal immunization with purified OmpA elicited protective immunity against infections caused by multidrug-resistant Acinetobacter baumannii.
abstract: Multidrug-resistant Acinetobacter baumannii (A. baumannii) is a rapidly emerging pathogen causing infections with high mortality rates due to inadequate medical treatment. New ways to prevent and treat such infections are of a critical medical need. In this study, intranasal vaccination with A. baumannii outer membrane protein A (OmpA) induced both systemic and mucosal antibodies. After challenge intraperitoneally by clinical strains of multidrug-resistant A. baumannii, mice immunized with OmpA had a significantly higher survival rate than control mice. The OmpA protein level tested positive by western blot in clinical strains of A. baumannii. Furthermore, characterization of human sera for anti-OmpA immunoglobulin G (IgG) antibody levels demonstrated that OmpA protein was immunogenic in healthy individuals and patients with A. baumannii invasive infections. In conclusion, to the best of our knowledge, this is the first study protective efficacy of mucosal immunization with OmpA as a protein antigen against multidrug-resistant A. Baumannii.
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Tianxiang
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jide
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dai
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. Electronic address: lipingzhang723@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000151:Acinetobacter Infections / Q000276:immunology / Q000517:prevention & control*; D040981:Acinetobacter baumannii / Q000276:immunology*; D000281:Administration, Intranasal; D058956:Administration, Mucosal; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001769:Blood / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D018928:Immunity, Mucosal; D051379:Mice; D016019:Survival Analysis; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D022223:Vaccines, Subunit; C078167:OMPA outer membrane proteins
keywords: A. Baumannii; Protein; Vaccine