title: Reduced graphene oxide-yttria nanocomposite modified electrode for enhancing the sensitivity of electrochemical genosensor.
abstract: Reduced graphene oxide-yttria nanocomposite (rGO:Y) is applied as electrochemical genosensor platform for ultrahigh sensitive detection of breast cancer 1 (BRCA1) gene for the first time. The sensor is based on the sandwich assay in which gold nanoparticle cluster labeled reporter DNA hybridize to the target DNA. Glassy carbon electrode modified with rGO-yttria serves as the immobilization platform for capture probe DNA. The sensor exhibited a fine capability of sensing BRCA1 gene with linear range of 10attomolar (aM) to 1nanomolar (nM) and a detection limit of 5.95attomolar. The minimum distinguishable response concentration is down to the attomolar level with a high sensitivity and selectivity. We demonstrated that the use of rGO:Y modified electrode along with gold nanoparticle cluster (AuNPC) label leads to the highly sensitive electrochemical detection of BRCA1 gene.
authors.affiliation: Nanoscience Research Laboratory, School of Nano Science and Technology, National Institute of Technology Calicut, Calicut, Kerala, India.
authors.forename: P Abdul
authors.initials: PA
authors.lastname: Rasheed
authors.affiliation: Nanoscience Research Laboratory, School of Nano Science and Technology, National Institute of Technology Calicut, Calicut, Kerala, India.
authors.forename: Thulasi
authors.initials: T
authors.lastname: Radhakrishnan
authors.affiliation: Nanoscience Research Laboratory, School of Nano Science and Technology, National Institute of Technology Calicut, Calicut, Kerala, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Shihabudeen
authors.affiliation: Nanoscience Research Laboratory, School of Nano Science and Technology, National Institute of Technology Calicut, Calicut, Kerala, India. Electronic address: sandhya@nitc.ac.in.
authors.forename: N
authors.initials: N
authors.lastname: Sandhyarani
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D019398:Genes, BRCA1; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D015019:Yttrium / Q000737:chemistry*
chemical_list: D015019:Yttrium; D006046:Gold; D006108:Graphite; C091417:yttria
keywords: Attomolar detection; BRCA1 gene; Electrochemical genosensor; Gold nanoparticle cluster; Nanocomposite; Reduced graphene oxide; Yttria