title: Gene expression profiles of autophagy-related genes in multiple sclerosis.
abstract: Multiple sclerosis (MS) is an imflammatory disease of central nervous system caused by genetic and environmental factors that remain largely unknown. Autophagy is the process of degradation and recycling of damaged cytoplasmic organelles, macromolecular aggregates, and long-lived proteins. Malfunction of autophagy contributes to the pathogenesis of neurological diseases, and autophagy genes may modulate the T cell survival. We aimed to examine the expression levels of autophagy-related genes. The blood samples of 95 unrelated patients (aged 17-65years, 37 male, 58 female) diagnosed as MS and 95 healthy controls were used to extract the RNA samples. After conversion to single stranded cDNA using polyT priming: the targeted genes were pre-amplified, and 96×78 (samples×primers) qRT-PCR reactions were performed for each primer pair on each sample on a 96.96 array of Fluidigm BioMark™. Compared to age- and sex-matched controls, gene expression levels of ATG16L2, ATG9A, BCL2, FAS, GAA, HGS, PIK3R1, RAB24, RGS19, ULK1, FOXO1, HTT were significantly altered (false discovery rate<0.05). Thus, altered expression levels of several autophagy related genes may affect protein levels, which in turn would influence the activity of autophagy, or most probably, those genes might be acting independent of autophagy and contributing to MS pathogenesis as risk factors. The indeterminate genetic causes leading to alterations in gene expressions require further analysis.
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Medical Biology, 27310 Sahinbey, Gaziantep, Turkey. Electronic address: mehriigci@gmail.com.
authors.forename: Mehri
authors.initials: M
authors.lastname: Igci
authors.affiliation: New York University Cancer Institute, New York University Brain Tumor Center, New York University Langone Medical Center, New York, NY 10016, USA.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Baysan
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Neurology, 27310 Sahinbey, Gaziantep, Turkey.
authors.forename: Remzi
authors.initials: R
authors.lastname: Yigiter
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Medical Biology, 27310 Sahinbey, Gaziantep, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ulasli
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Neurology, 27310 Sahinbey, Gaziantep, Turkey.
authors.forename: Sirma
authors.initials: S
authors.lastname: Geyik
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Medical Biology, 27310 Sahinbey, Gaziantep, Turkey.
authors.forename: Recep
authors.initials: R
authors.lastname: Bayraktar
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Medical Biology, 27310 Sahinbey, Gaziantep, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Bozgeyik
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Medical Biology, 27310 Sahinbey, Gaziantep, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Bozgeyik
authors.affiliation: Firat University, Elazıg Health College, Elazıg, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Bayram
authors.affiliation: Gaziantep University, Faculty of Medicine, Departments of Medical Biology, 27310 Sahinbey, Gaziantep, Turkey.
authors.forename: Ecir Ali
authors.initials: EA
authors.lastname: Cakmak
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000071182:Autophagosomes; D001343:Autophagy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D059467:Transcriptome; D055815:Young Adult
keywords: Autophagy; Gene expression analysis; Multiple sclerosis