title: Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ.
abstract: Techniques for in situ localization of gene products provide indispensable information for understanding biological function. In the case of enzymes, biological function is directly related to activity, and therefore, knowledge of activity patterns is central to understanding the molecular controls of plant development. We have previously developed a novel type of fluorogenic substrate for revealing glycoside hydrolase activity in planta, based on resorufin β-glycosides Here, we explore a wider range of such substrates to visualize glycoside hydrolase activities in Arabidopsis inflorescence stems in real time, especially highlighting distinct distribution patterns of these activities in the secondary cell walls of sclerenchyma cells. The results demonstrate that β-1,4-glucosidase, β-1,4-glucanase and β-1,4-galactosidase activities accompany secondary wall deposition. In contrast, xyloglucanase activity follows a different pattern, with the highest signal observed in mature cells, concentrated in the middle lamella. These data further the understanding of the process of cell wall deposition and function in sclerenchymatic tissues of plants.
authors.affiliation: Institute of Experimental Biology, University of Wroclaw, 50-328 Wroclaw, Poland. alicja.banasiak@uni.wroc.pl.
authors.forename: Alicja
authors.initials: A
authors.lastname: Banasiak
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden. ibatullin@omrb.pnpi.spb.ru.|Biophysics Division, Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute, Gatchina 188300, Russia. ibatullin@omrb.pnpi.spb.ru.
authors.forename: Farid M
authors.initials: FM
authors.lastname: Ibatullin
authors.affiliation: Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden. brumer@msl.ubc.ca.|Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z1, Canada. brumer@msl.ubc.ca.|Department of Chemistry, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z1, Canada. brumer@msl.ubc.ca.
authors.forename: Harry
authors.initials: H
authors.lastname: Brumer
authors.affiliation: Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea Plant Science Centre, 90183 Umea, Sweden. ewa.mellerowicz@slu.se.
authors.forename: Ewa J
authors.initials: EJ
authors.lastname: Mellerowicz
keywords: cell wall; glycoside hydrolase activity; in situ activity; sclerenchyma; wood; xylem