title: Down-regulation of microRNA-126-5p contributes to overexpression of VEGFA in lipopolysaccharide-induced acute lung injury.
abstract: OBJECTIVE
  To investigate the role of microRNA-126-5p (miR-126-5p) in acute lung injury induced by bronchial instillation of lipopolysaccharide (LPS), and to explore the potential target(s) of miR-126-5p in acute lung injury.
  
  
  RESULTS
  In the mice with LPS-induced acute lung injury, the level of miR-126-5p in the pulmonary tissues was decreased by 41 % whilst pulmonary vascular endothelial growth factor-A (VEGFA) doubled in its mRNA content and increased threefold in its protein level. Similar results were observed in the alveolar type II (ATII) cells treated with LPS. By using luciferase reporter assay, we found that miR-126-5p inhibited VEGFA expression by targeting its 3'-untranslated region. In addition, overexpression of miR-126-5p attenuated LPS-induced reduction of epithelial sodium channel and aquaporin 1 in ATII cells
  
  
  CONCLUSIONS
  MiR-126-5p was down-regulated in LPS-induced acute lung injury in mice. Thus overexpression of miR-126-5p may alleviate acute lung injury by down-regulating VEGFA.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, People's Republic of China.
authors.forename: Rurong
authors.initials: R
authors.lastname: Tang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Pei
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Bai
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, People's Republic of China. jk_wang2015@sina.com.
authors.forename: Junke
authors.initials: J
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015536:Down-Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D035683:MicroRNAs / Q000187:drug effects / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A
keywords: Acute lung injury; Alveolar type II cell; Lipopolysaccharide; MicroRNA-126-5p; Vascular endothelial growth factor-A