title: Canine Circovirus 1 (CaCV-1) and Canine Parvovirus 2 (CPV-2): Recurrent Dual Infections in a Papillon Breeding Colony.
abstract: Recurrent outbreaks of sudden death and bloody diarrhea were reported in March 2013 and February 2014 in a breeding colony of Papillon dogs. During the first outbreak, 1 adult dog and 2 eight-month-old puppies died. During the second outbreak, 2 ten-week-old puppies died. One puppy from the first outbreak and 2 puppies from the second outbreak were examined at necropsy. Histologically, all 3 puppies had severe segmental crypt necrosis of the small intestine and marked lymphoid follicle depletion in the spleen and Peyer's patches. Real-time (RT) polymerase chain reaction (PCR) demonstrated abundant canine parvovirus (CPV-2) DNA (Ct<15) in the affected small intestine, and immunohistochemistry detected large amounts of CPV-2 antigen in intestinal crypt epithelium and Kupffer cells but few positive macrophages in lymphoid organs. All puppies had marked sinusoidal histiocytosis and multifocal granulomatous inflammation in mesenteric lymph nodes and spleen, prompting additional RT-PCR testing for canine circovirus 1 (CaCV-1). Very high levels of CaCV-1 DNA (Ct<13) were detected in small intestine, lymph nodes, and spleen. In situ hybridization for CaCV-1 detected rare positive nuclei of regenerating crypt epithelium but abundant amounts of CaCV-1 nucleic acid in the cytoplasm and nuclei of histiocytes in all lymphoid tissues, including granulomatous inflammatory foci and hepatic Kupffer cells. Significant levels of CaCV-1 DNA were detected in blood and serum (Ct as low as 13) but not feces from 3 surviving dogs at 2 months or 1 year after the outbreak, respectively. We hypothesize that CPV-2 infection predisposed dogs to CaCV-1 infection and ultimately resulted in more severe clinical disease.
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI, USA.
authors.forename: T
authors.initials: T
authors.lastname: Thaiwong
authors.affiliation: Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Wise
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI, USA.
authors.forename: R K
authors.initials: RK
authors.lastname: Maes
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI, USA.
authors.forename: T
authors.initials: T
authors.lastname: Mullaney
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI, USA kiupel@dcpah.msu.edu.
authors.forename: M
authors.initials: M
authors.lastname: Kiupel
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000150:complications / Q000662:veterinary* / Q000821:virology; D017925:Circovirus; D060085:Coinfection / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D007421:Intestine, Small / Q000473:pathology / Q000821:virology; D007728:Kupffer Cells / Q000473:pathology / Q000821:virology; D010322:Parvoviridae Infections / Q000150:complications / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012008:Recurrence
keywords: canine circovirus; canine parvovirus type 2; dog circovirus; dogs; dual infection; emerging infectious diseases; recurrent outbreak