title: Efferocytosis as a regulator of macrophage chemokine receptor expression and polarization.
abstract: Efferocytosis has been suggested to promote macrophage resolution programs that are dependent on motility and emigration, however, few studies have addressed directed migration in resolving macrophages. In this report, we hypothesized that efferocytosis would induce differential chemokine receptor expression. Polarized macrophage populations, including macrophages actively engaged in efferocytosis, were characterized by PCR array and traditional transwell motility assays. We identified specific up-regulation of chemokine receptor CXCR4 on both mouse and human macrophages and characterized in vivo expression of CXCR4 in a resolving model of murine peritonitis. Using adoptive transfer and AMD3100 blocking, we confirmed a role for CXCR4 in macrophage egress to draining lymphatics. Collectively these data provide an important mechanistic link between efferocytosis and macrophage emigration.
authors.affiliation: Coulter Department of Biomedical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA.|Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Julianty
authors.initials: J
authors.lastname: Angsana
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.|Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
authors.forename: Jiaxuan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Liying
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.|Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Haller
authors.affiliation: Coulter Department of Biomedical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA.|Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.|Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
authors.forename: Elliot L
authors.initials: EL
authors.lastname: Chaikof
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D056747:Immunomodulation / Q000235:genetics / Q000276:immunology; D008262:Macrophage Activation / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism*; D010538:Peritonitis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis / Q000235:genetics* / Q000276:immunology*; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D018925:Chemokines; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine
keywords: Chemotaxis; Efferocytosis; Macrophage activation; Peritonitis; Resolution of inflammation