title: Antilipidemic effects and gene expression profiling of the glycosaminoglycans from cricket in rats on a high fat diet.
abstract: Glycosaminoglycan (GAG) from cricket (Gryllus bimaculatus) was studied as a potential health supplement. Antiatherosclerotic and antilipidemic effects of the GAG of G. bimaculatus (GbG, 5 or 10 mg/kg) were investigated in 15-week old Wistar rats treated with GbG for over a month. GbG produced a meaningful anti-edema effect with inhibition of C-reactive protein (CRP). Also, the weights of abdominal and epididymidal fat were also reduced in conjunction with a mild increase in body weight. Furthermore, the sero-biochemical parameters showed an antihyperlipidemic effect with decreased levels of phospholipid, AST, ALT, total cholesterol and glucose in a dose-dependent manner. In addition anticoagulant and antithrombotic effects were seen: platelet, thrombin time, prothrombin time and Factor I were increased with GbG treatment. Furthermore, the GbG treated rat group (at 10 mg/kg) compared to control, showed that 588 genes (test/control ratio >2.0) including lipocalin 2 (Lcn2) and alpha 2-macroglobulin (A2 m) were up-regulated, and 569 genes (test/control ratio >0.5) including stearoyl-coenzyme A desaturase 1 (Scd1) were down-regulated. Based on these results, GbG could potentially prevent or treat fatty liver or hyperlipidemia in rats on a high-fat diet.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-Gun, 55365, South Korea. amy@korea.kr.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Ahn
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-Gun, 55365, South Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 166 Nongsaengmyung-Ro, Iseo-Myun, Wanju-Gun, 55365, South Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Pharmacogenechips Inc., Chuncheon, 200-160, South Korea.
authors.forename: Kun-Koo
authors.initials: KK
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects* / Q000502:physiology; D059305:Diet, High-Fat / Q000009:adverse effects*; D005227:Fatty Acids / Q000037:antagonists & inhibitors / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D006025:Glycosaminoglycans / Q000302:isolation & purification / Q000494:pharmacology*; D006135:Gryllidae; D000960:Hypolipidemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005227:Fatty Acids; D006025:Glycosaminoglycans; D000960:Hypolipidemic Agents
keywords: Antilipidemic effect; G. bimaculatus; Glycosaminoglycan; High fat diet; Wistar rats