title: Low-temperature derivatization followed by vortex-assisted liquid-liquid microextraction for the analysis of polyamines in Nicotiana Tabacum.
abstract: Polyamines are ubiquitous polycationic molecules that play a key role in many biological processes such as nucleic acid metabolism, protein synthesis, cell growth, and nicotine synthesis precursors. This work describes a rapid, sensitive, convenient, green, and cost-effective method for the determination of polyamines in Nicotiana tabacum by ultra high performance liquid chromatography with photodiode array detection. The analytes were derivatized with 3,5-dinitrobenzoyl chloride at low temperature (about 4°C) and then extracted with vortex-assisted liquid-liquid microextraction. The experimental designs based on quarter-fractional factorial design and Doehlert design were used to screen and optimize the important factors in microextraction process. Under the optimal conditions, the method was linear over 0.05-8.00 μg/mL with an r(2) ≥ 0.992 and exhibited good repeatability and reproducibility less than 6.0 and 6.9%, respectively. The limit of detection ranged between 0.013 and 0.029 μg/g. The newly developed method was successfully employed to analyze different leaf samples of Nicotiana tabacum, among which the polyamines contents were found to be very different. Moreover, tyramine, 1,3-diaminopropane, homospermidine, and canavalmine were tentatively identified with the electrospray ionization quadrupole time-of-flight mass spectrometry. To our knowledge, this is the first report of identification of canavalmine in Nicotiana Tabacum.
authors.affiliation: Guizhou Academy of Tobacco Science, Guiyang, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Cai
authors.affiliation: Guizhou Academy of Tobacco Science, Guiyang, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Cai
authors.affiliation: Guizhou Academy of Tobacco Science, Guiyang, P.R. China.
authors.forename: Zhangmin
authors.initials: Z
authors.lastname: Xiang
authors.affiliation: Guizhou Academy of Tobacco Science, Guiyang, P.R. China.
authors.forename: Huina
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Tobacco Technology Promotion Station, Zunyi County Tobacco Company of Guizhou Province, Zunyi, P.R. China.
authors.forename: Xingyi
authors.initials: X
authors.lastname: Rao
authors.affiliation: Guizhou Academy of Tobacco Science, Guiyang, P.R. China.|Upland Flue-Cured Tobacco Quality & Ecology Key Laboratory of China Tobacco.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Pan
authors.affiliation: Guizhou Academy of Tobacco Science, Guiyang, P.R. China.|Key Laboratory of Molecular Genetics, CNTC, Guiyang, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lei
mesh_terms: D059627:Liquid Phase Microextraction; D008968:Molecular Conformation; D011073:Polyamines / Q000032:analysis*; D013696:Temperature; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D011073:Polyamines
keywords: Doehlert design; Nicotiana tabacum; Polyamines; Tandem mass spectrometry; Vortex-assisted liquid-liquid microextraction