title: Fucoidan Extracted from Hijiki Protects Brain Microvessel Endothelial Cells Against Diesel Exhaust Particle Exposure-Induced Disruption.
abstract: This study was performed to evaluate the protective effects of fucoidan against the decreased function of primary cultured bovine brain microvessel endothelial cells (BBMECs) after exposure to diesel exhaust particles (DEPs). BBMECs were extracted from bovine brains and cultured until confluent. To evaluate the function of BBMECs, we performed a permeability test using cell-by-cell equipment and by Western blot analysis for zonular occludens-1 (ZO-1), which is a tight junction protein of BMECs, and evaluated oxidative stress in BBMECs using the DCFH-DA assay and the CUPRAC-BCS assay. The increased oxidative stress in BBMECs following DEP exposure was suppressed by fucoidan. In addition, permeability of BBMECs induced by DEP exposure was decreased by fucoidan treatment. Our results showed that fucoidan protects against BBMEC disruption induced by DEP exposure. This study provides evidence that fucoidan might protect the central nervous system (CNS) against DEP exposure.
authors.affiliation: 1 Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University , Cheongju, Korea.
authors.forename: Young-Sook
authors.initials: YS
authors.lastname: Choi
authors.affiliation: 1 Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University , Cheongju, Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Eom
authors.affiliation: 1 Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University , Cheongju, Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Kim
authors.affiliation: 2 Neurochemistry Laboratory, Division of Neurotoxicology, National Center of Toxicological Research/Food and Drug Administration , Jefferson, Arkansas, USA.
authors.forename: Syed F
authors.initials: SF
authors.lastname: Ali
authors.affiliation: 3 Department of Medicine, University of California at Irvine , Irvine, California, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Kleinman
authors.affiliation: 1 Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University , Cheongju, Korea.
authors.forename: Yong-Dae
authors.initials: YD
authors.lastname: Kim
authors.affiliation: 1 Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University , Cheongju, Korea.
authors.forename: Heon
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D055806:Microvessels / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D012624:Seaweed / Q000737:chemistry*; D001335:Vehicle Emissions / Q000633:toxicity*; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D020011:Protective Agents; D001335:Vehicle Emissions; D062826:Zonula Occludens-1 Protein; C007789:fucoidan
keywords: BBB disruption; bovine brain microvessel endothelial cells (BBMECs); diesel exhaust particles (DEPs); fucoidan; tight junction proteins