title: A novel label-free microfluidic paper-based immunosensor for highly sensitive electrochemical detection of carcinoembryonic antigen.
abstract: In this work, a highly sensitive label-free paper-based electrochemical immunosensor employing screen-printed working electrode (SPWE) for detection of carcinoembryonic antigen (CEA) was fabricated. In order to raise the detection sensitivity and immobilize anti-CEA, amino functional graphene (NH2-G)/thionine (Thi)/gold nanoparticles (AuNPs) nanocomposites were synthesized and coated on SPWE. The principle of the immunosensor determination was based on the fact that the decreased response currents of Thi were proportional to the concentrations of corresponding antigens due to the formation of antibody-antigen immunocomplex. Experimental results revealed that the immunoassay enabled the determination of standard CEA solutions with linear working ranges of 50pgmL(-1) to 500ngmL(-1), the limit of detections for CEA is 10pgmL(-1) (S/N=3) and its corresponding correlation coefficients were 0.996. Furthermore, the proposed immunosensor could be used for the determination of clinical serum samples. A large number of clinical serum samples were detected and the relative errors between measured values and reference concentrations were calculated. Results showed that this novel paper-based electrochemical immunosensor could provide a new platform for low cost, sensitive, specific, and point-of-care diagnosis in cancer detection.
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Huiren
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Yan
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China. Electronic address: xxcai@mail.ie.ac.cn.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Cai
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000097:blood*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010209:Paper; D019095:Point-of-Care Systems
chemical_list: D055910:Antibodies, Immobilized; D002272:Carcinoembryonic Antigen; D006046:Gold; D006108:Graphite
keywords: Graphene nanocomposites; Label-free; Microfluidic paper-based device; Screen-printing