title: [Anti-platelet aggregation bioassay based quality control for XST capsules].
abstract: A in vitro platelet aggregation bioassay was developed for the quality control of XST capsules. The in vitro anti-platelet aggregation effect in rats was observed to detect the bioactivity of XST capsules. Panax notoginseng saponins and Xuesaitong lyophilizedpowder for injection were taken as standard control substances to determine the potency. According to the results, XST capsules showeda significant inhibitory effect on thrombin-induced platelet aggregation in a dose-dependent manner. The in vitro anti-platelet activity oflyophilized powder for injection was stabler than that of Panax notoginseng saponins, and so suitable to serve as a standard control substance. The biological potency of XST capsules compared with standard control substance was detected by using parallel line assay. According to the results, the established bioassay method had a good repeatability (RSD 2.92%). The sample test results could pass thereliability test(linear deviation P > 0.05, parallel deviation P > 0.05). This bioassay method could be used as one of the complementary quality control methods for XST capsules.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Xin
authors.initials: X
authors.lastname: Mao
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Xiang
authors.forename: Yi-meng
authors.initials: YM
authors.lastname: Ge
authors.forename: Fu-long
authors.initials: FL
authors.lastname: Liao
authors.forename: Yun
authors.initials: Y
authors.lastname: You
mesh_terms: D000818:Animals; D002214:Capsules / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D053723:Panax notoginseng / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D010975:Platelet Aggregation Inhibitors; D012503:Saponins