title: Unsupervised Identification of Isotope-Labeled Peptides.
abstract: In vivo isotopic labeling coupled with high-resolution proteomics is used to investigate primary metabolism in techniques such as stable isotope probing (protein-SIP) and peptide-based metabolic flux analysis (PMFA). Isotopic enrichment of carbon substrates and intracellular metabolism determine the distribution of isotopes within amino acids. The resulting amino acid mass distributions (AMDs) are convoluted into peptide mass distributions (PMDs) during protein synthesis. With no a priori knowledge on metabolic fluxes, the PMDs are therefore unknown. This complicates labeled peptide identification because prior knowledge on PMDs is used in all available peptide identification software. An automated framework for the identification and quantification of PMDs for nonuniformly labeled samples is therefore lacking. To unlock the potential of peptide labeling experiments for high-throughput flux analysis and other complex labeling experiments, an unsupervised peptide identification and quantification method was developed that uses discrete deconvolution of mass distributions of identified peptides to inform on the mass distributions of otherwise unidentifiable peptides. Uniformly (13)C-labeled Escherichia coli protein was used to test the developed feature reconstruction and deconvolution algorithms. The peptide identification was validated by comparing MS(2)-identified peptides to peptides identified from PMDs using unlabeled E. coli protein. Nonuniformly labeled Glycine max protein was used to demonstrate the technology on a representative sample suitable for flux analysis. Overall, automatic peptide identification and quantification were comparable or superior to manual extraction, enabling proteomics-based technology for high-throughput flux analysis studies.
authors.affiliation: Biotechnology Institute, University of Minnesota , Saint Paul, Minnesota 55108, United States.
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Goldford
authors.affiliation: Biotechnology Institute, University of Minnesota , Saint Paul, Minnesota 55108, United States.|Department of Plant Biology, 1500 Gortner Avenue, University of Minnesota , Saint Paul, Minnesota 55108, United States.
authors.forename: Igor G L
authors.initials: IG
authors.lastname: Libourel
mesh_terms: D000465:Algorithms; D002247:Carbon Isotopes; D029968:Escherichia coli Proteins / Q000032:analysis / Q000378:metabolism; D007553:Isotope Labeling; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D029968:Escherichia coli Proteins; D010455:Peptides; D010940:Plant Proteins