title: Co-circulation of H5N6, H3N2, H3N8, and Emergence of Novel Reassortant H3N6 in a Local Community in Hunan Province in China.
abstract: Multiple infections of avian influenza viruses (AIVs) in poultry or wild birds contribute to the continued evolution of H5 subtype viruses in nature and provide potential recombination of AIVs of different origins. In this study, we carried out surveillance of AIVs in ducks, geese and the environment of a community in Hunan province, China, from 2014-2015. We isolated multiple co-circulated AIVs including H3N2, H3N8, and H5N6, and, most importantly, a novel reassortant: H3N6. Phylogenetic analyses suggest that H3N6 is highly likely derived from H5N6, which has recently been shown to have zoonotic potential with human infections. Studies with mammalian cell lines and a mouse model indicate that four selected AIVs of duck or goose origin can infect MDCK and A549 cells but have low pathogenicity in mice. We propose that a potential co-circulation of multiple subtypes including H5N6 in local area may result in the production of novel subtypes such as H3N6 by gene reassortment.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Xuyong
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Jiayun
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Yane
authors.initials: Y
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Shaoting
authors.initials: S
authors.lastname: Weng
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Maijuan
authors.initials: M
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857.
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, China.|Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857.
authors.forename: Jie
authors.initials: J
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, China.
authors.forename: Hualan
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, 1 Xujiaping, Lanzhou 730046, China.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China / Q000453:epidemiology; D004195:Disease Models, Animal; D004285:Dogs; D004372:Ducks; D004783:Environmental Microbiology; D019143:Evolution, Molecular; D005777:Geese; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000453:epidemiology* / Q000821:virology*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000821:virology; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D014774:Virulence