title: Isolation, characterization and multi-lineage differentiation of stem cells from human exfoliated deciduous teeth.
abstract: The aim of the present study was to isolate stem cells from human exfoliated deciduous teeth (SHEDs) and identify their phenotypes and multi‑lineage differentiation potential. Three SHED cell strains were successfully isolated from three exfoliated deciduous teeth from different human subjects using the outgrowth method. Flow cytometric analysis indicated that SHEDs displayed high expression of the mesenchymal cell markers CD73 and CD90 but low expression of the hematopoietic stem cell marker CD34. PCR analysis illustrated that SHEDs expressed the mesenchymal stem cell markers CD44, CD73 and CD90, the osteoblast markers Alpl, Runx2, CBFA1 and collagen Ⅰ, the cartilage cell markers Col10a1 and Acan, the adipose cell markers PPARγ2 and LPL, and the neuronal stem cell marker Nestin. In vitro induction experiments demonstrated the potential of the SHEDs for osteogenic, adipogenic and neurogenic differentiation. These SHED cells may be useful for further stem cell research and future therapeutic applications.
authors.affiliation: Centre for Stem Cells and Regenerative Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Baoxing
authors.initials: B
authors.lastname: Chen
authors.affiliation: Centre for Stem Cells and Regenerative Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Centre for Stem Cells and Regenerative Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Stomatology, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Samuel Qinnan
authors.initials: SQ
authors.lastname: Deng
authors.affiliation: Department of Stomatology, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Mental Disorders, Research Center for Sectional and Imaging Anatomy, Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China.
authors.forename: Shuwei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Centre for Stem Cells and Regenerative Medicine, Liaocheng People's Hospital/Affiliated Liaocheng Hospital, Taishan Medical University, Liaocheng, Shandong 252000, P.R. China.
authors.forename: Fabin
authors.initials: F
authors.lastname: Han
mesh_terms: D050156:Adipogenesis; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002648:Child; D020219:Chondrogenesis; D003782:Dental Pulp / Q000166:cytology; D006801:Humans; D055495:Neurogenesis; D010012:Osteogenesis; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014094:Tooth, Deciduous / Q000166:cytology*