title: Investigation of an SPR biosensor for determining the influence of connexin 43 expression on the cytotoxicity of cisplatin.
abstract: The real-time and label free detection abilities of surface plasmon resonance (SPR) biosensors provide a way of evaluating the influence of some genes' expression on anti-tumor drug cytotoxicity. However, studies in this field are lacking. Connexin 43 is a tumor suppressor gene and the mechanism of its effect in cisplatin cytotoxicity is still unclear. A phase SPR biosensor was used to determine the influence of connexin 43 expression on cisplatin cytotoxicity in three cancer cell lines. The results showed that the SPR signal curves have two stages. In the first hour, the SPR signal shows dramatic changes which are related to connexin 43 expression. In the subsequent stage, the SPR signal slowly declines and is related to apoptosis. Comparison of SPR measurements from several conventional biological assays showed that connexin 43 expression can affect cellular response to cisplatin in the period of oxidative stress, and results in the cells being more sensitive to cisplatin. The conclusion is further confirmed by long-term SPR measurement results and cellular morphological changes.
authors.affiliation: Tianjin Union Medical Center, Tianjin, 300121, China. junliu_sci@163.com.
authors.forename: Yijia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Tianjin Union Medical Center, Tianjin, 300121, China. junliu_sci@163.com.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Tianjin Union Medical Center, Tianjin, 300121, China. junliu_sci@163.com.
authors.forename: Chunze
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Tianjin Union Medical Center, Tianjin, 300121, China. junliu_sci@163.com.
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Tianjin Union Medical Center, Tianjin, 300121, China. junliu_sci@163.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Tianjin Union Medical Center, Tianjin, 300121, China. junliu_sci@163.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xue
authors.affiliation: Tianjin Union Medical Center, Tianjin, 300121, China. junliu_sci@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Micro & Nano Optics, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China. xcyuan@szu.edu.cn.
authors.forename: X-C
authors.initials: XC
authors.lastname: Yuan
mesh_terms: D000072283:A549 Cells; D015374:Biosensing Techniques; D002450:Cell Communication; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D018031:Connexin 43 / Q000378:metabolism*; D017629:Gap Junctions; D006801:Humans; D020349:Surface Plasmon Resonance
chemical_list: D018031:Connexin 43; D002945:Cisplatin