title: Protective role of garlic oil against oxidative damage induced by furan exposure from weaning through adulthood in adult rat testis.
abstract: Furan is produced in a wide variety of heat-treated foods via thermal degradation. Furan contamination is found to be relatively high in processed baby foods, cereal products, fruits juices, and canned vegetables. Several studies have demonstrated that furan is a potent hepatotoxin and hepatocarcinogen in rodents. However, few studies have investigated the toxic effects of furan in the testis. In addition, the exact mechanism(s) by which furan exerts toxicity in the testis has not been fully elucidated. In this study, we investigated the potential of furan exposure from weaning through adulthood to induce oxidative stress in adult rat testis, as well as the potential of garlic oil (GO) to ameliorate the induced toxicity. Our results reveal that furan administration significantly reduced serum testosterone levels and increased the levels of malondialdehyde (MDA); furthermore, furan administration decreased significantly the enzymatic activity of testicular antioxidants, including glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) and induced histopathological alterations in the testis. GO co-administration ameliorated the reduction in testosterone levels and dramatically attenuated the furan-induced oxidative and histopathological changes. In addition, Go significantly down-regulated the increased caspase-3 and cytochrome P450 2E1 (CYP2E1) expression in the furan-treated testis. To the best of our knowledge, this study is the first to demonstrate the furan-induced oxidative changes in the adult rat testis and the protective role of GO to ameliorate these changes through its antioxidant effects and its ability to inhibit CYP2E1 production.
authors.affiliation: Menoufia University, Department of Anatomy and Embryology, Faculty of Medicine, Menoufia, Egypt. Electronic address: gehanel-akabawy@med.menofia.edu.eg.
authors.forename: Gehan
authors.initials: G
authors.lastname: El-Akabawy
authors.affiliation: Menoufia University, Department of Anatomy and Embryology, Faculty of Medicine, Menoufia, Egypt. Electronic address: Nevien.hefzi@med.menofia.edu.eg.
authors.forename: Neveen M
authors.initials: NM
authors.lastname: El-Sherif
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004789:Enzyme Activation; D005663:Furans / Q000633:toxicity*; D008297:Male; D018384:Oxidative Stress; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D014886:Weaning
chemical_list: D000498:Allyl Compounds; D000975:Antioxidants; D002273:Carcinogens; D005663:Furans; D013440:Sulfides; D013739:Testosterone; C038491:allyl sulfide; D019392:Cytochrome P-450 CYP2E1; C505410:Casp3 protein, rat; D053148:Caspase 3; C039281:furan
keywords: CYP2E1; Caspase-3; Furan; Histopathology; Oxidative stress; Rat testis