title: Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity.
abstract: During a screening program for antimicrobial compounds from underexplored habitats, a Gram-positive bacterium TD-032, was isolated from arid soil, Thar Desert (India), and analyzed for its morphological, physicochemical, and antimicrobial properties. The 16S ribosomal DNA (rDNA) sequence of the isolate was further studied for the novelty of γ-hyper variable region. TD-032 was grown in large-scale culture, and aqueous and organic solvent extracts analyzed for antimicrobial activity. Culture characteristics showed a lack of diffusible and melanoid pigments. The morphological features were pale yellow aerial mycelium colony color with brownish yellow substrate mycelium and leathery texture. The isolate could grow at 1% concentration of sodium chloride, temperature of 40°C, and a wide range of pH (7.0-12.0). An evaluation for extracellular enzymatic activities showed secretion of gelatinase(s), cellulase(s), and lipase(s). The γ-hyper variable region of 16S rDNA sequence of TD-032 showed 98.33% relatedness to Yuhushiella deserti, indicating a potential new species. Aqueous and ethyl acetate extracts showed antimicrobial activity against Gram-positive and Gram-negative bacteria inclusive clinical isolates. Inhibition of both test bacteria suggests that TD-032 produces a broad spectrum of antimicrobial substances.
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
authors.forename: A
authors.initials: A
authors.lastname: Ibeyaima
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Rana
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
authors.forename: Anuj
authors.initials: A
authors.lastname: Dwivedi
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, Pushpanjali Crosslay Hospital, Ghaziabad, Uttar Pradesh, India.
authors.forename: Narendra
authors.initials: N
authors.lastname: Saini
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
authors.forename: Indira P
authors.initials: IP
authors.lastname: Sarethy
keywords: Actinomycetes; Thar Desert; antimicrobial activity; hyper-variable regions/16S ribosomal DNA