title: Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA.
abstract: During a survey of apparently healthy baitfish from the state of Minnesota, a novel piscine-myocarditis-like virus (PMCLV) was detected in golden shiners (Notemigonus crysoleucas). The nearly complete genome sequence is 5819 nt long, including a partial 5' untranslated region (UTR) of 100 nt. The sequence is divided into three ORFs: the complete ORF1 and ORF2, encoding proteins of 818 and 831 amino acids, respectively, and a partial ORF3 encoding 248 amino acids of the corresponding protein. This novel virus sequence was most closely related to piscine myocarditis virus (PMCV), with a 49.0 % and 58.2 % amino acid identity in the ORF1 (YP005481249)- and ORF2 (YP004581250)-encoded proteins, respectively. Six of 56 retail outlets (e.g., bait shops) were positive during the 2014-2015 survey, indicating a 10.7 % prevalence of the novel virus in this population of golden shiners. Currently, there is no disease that is known to be associated with this virus.
authors.affiliation: Minnesota Veterinary Diagnostic Laboratory, 1333 Gortner Avenue, St. Paul, MN, 55108, USA. kumars@umn.edu.|Department of Veterinary Population Medicine, University of Minnesota, 1365 Gortner Avenue, St. Paul, MN, 55108, USA. kumars@umn.edu.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Mor
authors.affiliation: Minnesota Veterinary Diagnostic Laboratory, 1333 Gortner Avenue, St. Paul, MN, 55108, USA.|Department of Veterinary Population Medicine, University of Minnesota, 1365 Gortner Avenue, St. Paul, MN, 55108, USA.
authors.forename: Nicholas B D
authors.initials: NB
authors.lastname: Phelps
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000821:virology*; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D019196:Totiviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins