title: Simultaneous determination of plant growth regulator and pesticides in bean sprouts by liquid chromatography-tandem mass spectrometry.
abstract: A simple and sensitive analytical method based on QuEChERS approach using liquid chromatography tandem mass spectrometry was developed and validated for the determination of 6-benzylaminopurine, carbendazim and thiabendazole in bean sprouts. Sodium chloride and sodium acetate were used for salting-out step and magnesium sulfate for clean-up. The validation of optimized method was satisfactory with recoveries, between 89.5% and 103.2% for the three compounds, and relative standard deviation (RSD) values less than 3.3% at 20 and 40ng/g fortification levels (n=5). Limit of detection (LOD) and limit of quantification (LOQ) was 2.1-3.7ng/g and 6.3-11.1ng/g, respectively. Monitoring of 126 bean sprout samples collected from local markets was performed to verify the adaptability in real samples. No pesticides were detected but 6-benzylaminopurine was found in 3 samples at the level of 15-20ng/g. The optimized method should be applicable for monitoring of 6-benzylaminopurine, carbendazim and thiabendazole in bean sprouts in short time.
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: kkg1229@korea.kr.
authors.forename: Kwang-Gon
authors.initials: KG
authors.lastname: Kim
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: vetpo2005@korea.kr.
authors.forename: Duck-Woong
authors.initials: DW
authors.lastname: Park
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: ggl21@korea.kr.
authors.forename: Gyung-Ri
authors.initials: GR
authors.lastname: Kang
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: kts2877@korea.kr.
authors.forename: Tae-Sun
authors.initials: TS
authors.lastname: Kim
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: youmeandus@korea.kr.
authors.forename: Yongshik
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: munsujin@korea.kr.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Moon
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: dmsdk22@korea.kr.
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Choi
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: drha57@korea.kr.
authors.forename: Dong-Ryong
authors.initials: DR
authors.lastname: Ha
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: keunsun@korea.kr.
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Kim
authors.affiliation: Agro-fishery Products Inspection Center, Health and Environment Research Institute of Gwangju, 16 Maewol-ro Seo-gu, Gwangju 61986, Republic of Korea. Electronic address: foodcbs@korea.kr.
authors.forename: Bae-Sik
authors.initials: BS
authors.lastname: Cho
mesh_terms: D001562:Benzimidazoles / Q000032:analysis; D002219:Carbamates / Q000032:analysis; D002853:Chromatography, Liquid; D007887:Fabaceae / Q000737:chemistry*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D010575:Pesticides / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis*; D015203:Reproducibility of Results; D019346:Sodium Acetate / Q000032:analysis; D012965:Sodium Chloride / Q000032:analysis; D053719:Tandem Mass Spectrometry; D013827:Thiabendazole / Q000032:analysis
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; D010575:Pesticides; D010937:Plant Growth Regulators; D012965:Sodium Chloride; D019346:Sodium Acetate; C006698:carbendazim; D013827:Thiabendazole
keywords: 6-Benzylaminopurine (PubChem CID: 62389); Bean sprouts; Carbendazim (PubChem CID: 25429); LC–MS/MS; Pesticides; Plant growth regulator; QuEChERS; Thiabendazole (PubChem CID: 5430)