title: Strain-Dependent Gene Expression during Mouse Embryonic Palate Development.
abstract: The effect of strain background on gene function in growth and development has been well documented. However, it has not been extensively reported whether the strain background affects the gene expression pattern. Here, we found that the expression of homeobox geneMeox-2and FGF receptor 1 geneFgfr1during mouse palate development is strain-dependent. On the C57B6 inbred background,Meox-2is expressed in the palatal outgrowth on Embryonic Day 11.5 (E11.5); the expression shifts posteriorly and is restricted to the back of palate on E14.5. On the Swiss Webster outbred background,Meox-2expression covers both anterior and posterior regions with the same intensity from E12.5 to E14.5. On the Black Swiss background,Meox-2expression also covers the entire palate A-P axis, but is much weaker in the anterior region on E14.5.Fgfr1also displays distinct expression patterns in the palatal outgrowth on E11.5 in these three strains. On the Black Swiss outbred background, the expression is restricted to the anterior palatal outgrowth. In marked contrast, the expression in the Swiss Webster outbred strain is located exclusively in the posterior palate outgrowth on E11.5, whereas in the C57B6 inbred strain, the expression is undetectable in the palatal outgrowth on E11.5.
authors.affiliation: Department of Molecular, Cellular and Craniofacial Biology and Birth Defects Center, University of Louisville School of Dentistry, Louisville, KY 40202, USA; Division of Cardiovascular Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA.
authors.forename: Jiu-Zhen
authors.initials: JZ
authors.lastname: Jin
authors.affiliation: Department of Molecular, Cellular and Craniofacial Biology and Birth Defects Center, University of Louisville School of Dentistry, Louisville, KY 40202, USA.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Ding
keywords: Black Swiss and C57B6; Fgfr1; Meox-2; Swiss Webster; secondary palate