title: Topical Moltkia coerulea hydroethanolic extract accelerates the repair of excision wound in a rat model.
abstract: OBJECTIVE
  To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment (MCO) on the healing of excision wound in a rat model.
  
  
  METHODS
  Circular surgical full thickness excision wound, with 314 mm² size, was induced in the anterior-dorsal side of each rat. Three different doses of MCO (1%, 3% and 6%) were administrated. On Day 3, 7, 14 and 21, the tissue was sampled and immune cells, fibroblasts and fibrocytes distribution per one mm² of wound area, collagen density and re-epithelialization were analyzed. Moreover, the total flavnoid, phenols and anti-oxidant potential of the MCO were evaluated. Ultimately, the percentage of wound contraction in different groups was compared with each other.
  
  
  RESULTS
  Hydroethanolic extract of MCO significantly (p < 0.05) increased wound contraction percentage. The animals in medium and high dose MCO-treated groups exhibited remarkably (p<0.05) higher fibroblast and fibrocyte distribution and significantly (p < 0.05) lower immune cells infiltration. On Day 7 after injury, MCO up-regulated neovascularization in a dose-dependent way.
  
  
  CONCLUSIONS
  Our data showed that MCO shortened the inflammation phase by provoking the fibroblast proliferation. Moreover, MCO promoted the healing process by up-regulating the angiogenesis and provoking the structural cells proliferation as well as increasing the collagen synthesis, cross-linking, and deposition.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Farahpour
authors.forename: Aydin
authors.initials: A
authors.lastname: Dilmaghanian
authors.forename: Maisam
authors.initials: M
authors.lastname: Faridy
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Karashi
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001707:Biopsy, Needle; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007492:Iran; D008297:Male; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries*; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D010936:Plant Extracts