title: Isolation and antimicrobial activities of actinobacteria closely associated with liquorice plants Glycyrrhiza glabra L. and Glycyrrhiza inflate BAT. in Xinjiang, China.
abstract: A total of 218 actinobacteria strains were isolated from wild perennial liquorice plants Glycyrrhiza glabra L. and Glycyrrhiza. inflate BAT. Based on morphological characteristics, 45 and 32 strains from G. inflate and G. glabra, respectively, were selected for further analyses. According to 16S rRNA sequence analysis, most of the strains belonged to genus Streptomyces and a few strains represented the rare actinobacteria Micromonospora, Rhodococcus and Tsukamurella. A total of 39 strains from G. inflate and 27 strains from G. glabra showed antimicrobial activity against at least one indicator organism. The range of the antimicrobial activity of the strains isolated from G. glabra and G. inflate was similar. A total of 34 strains from G. inflate and 29 strains from G. glabra carried at least one of the genes encoding polyketide synthases, non-ribosomal peptide synthetase and FADH2-dependent halogenase. In the type II polyketide synthase KSα gene phylogenetic analysis, the strains were divided into two major clades: one included known spore pigment production-linked KSα sequences and other sequences were linked to the production of different types of aromatic polyketide antibiotics. Based on the antimicrobial range, the isolates that carried different KSα types were not separated from each other or from the isolates that did not carry KSα. The incongruent phylogenies of 16S rRNA and KSα genes indicated that the KSα genes were possibly horizontally transferred. In all, the liquorice plants were a rich source of biocontrol agents that may produce novel bioactive compounds.
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.|Xinjiang Production & Construction Corps, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liao
authors.affiliation: Xinjiang Production & Construction Corps, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Xinjiang Production & Construction Corps, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Biotechnology Center, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Luzhou 646100, China.
authors.forename: Maoke
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Ao
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Yunfu
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Decong
authors.initials: D
authors.lastname: Liao
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Kaiwei
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Quanju
authors.initials: Q
authors.lastname: Xiang
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Xinjiang Production & Construction Corps, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, College of Resource, Sichuan Agricultural University, Yaan 625000, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Environmental Sciences, University of Helsinki, Helsinki, Fin-00014, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Penttinen
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D061046:Biological Control Agents / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D006035:Glycyrrhiza / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010453:Peptide Synthases / Q000235:genetics*; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D061046:Biological Control Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D048630:Polyketide Synthases; D010453:Peptide Synthases