title: Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation.
abstract: Micro-inflammation plays an important role in the pathogenesis of spontaneously hypertensive rat (SHR). In the present study, we investigated the therapeutic potential of resveratrol (RSV), a polyphenol with anti-fibrosis activity in hypertensive renal damage model. In SHR renal damage model, RSV treatment blunted the increase in urine albumin excretion, urinary β2-microglobulin (β2-MG), attenuated the decrease in creatinine clearance rate (CCR). The glomerular sclerosis index (1.54±0.33 compared with 0.36±0.07) and tubulointerstitial fibrosis (1.57±0.31 compared with 0.19±0.04) were significantly higher in SHRs compared with Wistar Kyoto rats (WKYs), which were significantly lower by RSV treatment. The increases in mesangium accumulation and the expression of renal collagen type I (Col I), fibronectin (Fn), plasminogen activator inhibitor-1 (PAI-1) and transforming growth factor-β1 (TGF-β1) in SHR were also reduced by RSV treatment. Nuclear factor κB (NF-κB) expression was increased in the cytoplasm and nuclei of the SHR kidneys, which was significantly decreased by RSV treatment. Furthermore, the protein level of IκB-α significantly decreased in the kidneys of the SHR when compared with the WKYs. RSV treatment partially restored the decreased IκB-α level. In SHR kidney, increased expression of interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein 1 (MCP-1) were observed. These changes were attenuated by RSV treatment. No changes in blood pressure were detected between SHR group and SHR + RSV group. Taken together, the present study demonstrated that RSV treatment may significantly attenuate renal damage in the SHR model of chronic kidney disease (CKD). The renal protective effect is associated with inhibition of IL-6, ICAM-1 and MCP-1 expression via the regulation of the nuclear translocation of NF-κB, which suggesting that micro-inflammation may be a potential therapeutic target of hypertensive renal damage.
authors.affiliation: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Xue
authors.affiliation: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Cao
authors.affiliation: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Fan
authors.affiliation: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China chenxl8448@sina.com huangxz421@126.com.
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China chenxl8448@sina.com huangxz421@126.com.
authors.forename: Xin-Zhong
authors.initials: XZ
authors.lastname: Huang
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D024042:Collagen Type I / Q000235:genetics; D004195:Disease Models, Animal; D005353:Fibronectins / Q000235:genetics; D005355:Fibrosis / Q000188:drug therapy* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D001613:beta 2-Microglobulin / Q000235:genetics
chemical_list: D024042:Collagen Type I; D005353:Fibronectins; D017395:Plasminogen Activator Inhibitor 1; D013267:Stilbenes; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D001613:beta 2-Microglobulin; D000077185:Resveratrol
keywords: inflammation; intercellular adhesion molecule-1 (ICAM-1); interleukin-6 (IL-6); monocyte chemoattractant protein 1 (MCP-1); nuclear factor κB (NF-κB); resveratrol (RSV); spontaneously hypertensive rat (SHR)