title: [Effect of melatonin instillations on the clinical course of experimental uveitis and biochemical processes in tears and aqueous humor].
abstract: Acute immunogenic uveitis was modeled in rabbits via the subcutaneous and intravitreal injections of normal horse serum. We studied the effect of instillations of 0.1% melatonin solution on the clinical course of uveitis and biochemical parameters of tear fluid and aqueous humor: antioxi-dant activity, protein concentration and α(2)-macroglobulin level. Melatonin instillations decreased clinical manifestations of uveitis. We found that the antioxidant activity in tears of the rabbits treated with melatonin was substantially higher and the α(2)-macroglobulin level lower than in untreated animals. Antioxidant activity in aqueous humor taken on day 10 of uveitis was also twice higher while protein and α(2)-macroglobulin levels were 1.5-2 times lower than in untreated animals. These data indicate that instillations of melatonin increase the local antioxidant activity and decrease the acuity of inflammation and permeability of hematoophthalmic barrier in uveitis.
authors.affiliation: Moscow Helmholtz Research Institute of Eye Diseases, Moscow, Russia.
authors.forename: N B
authors.initials: NB
authors.lastname: Chesnokova
authors.affiliation: Moscow Helmholtz Research Institute of Eye Diseases, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Beznos
authors.affiliation: Moscow State University, Chemistry faculty, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Lozinskaya
authors.affiliation: Moscow Helmholtz Research Institute of Eye Diseases, Moscow, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Beyshenova
authors.affiliation: Moscow Helmholtz Research Institute of Eye Diseases, Moscow, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Nesterova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001082:Aqueous Humor / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D011817:Rabbits; D013482:Superoxide Dismutase / Q000378:metabolism; D013666:Tears / Q000187:drug effects* / Q000378:metabolism; D014605:Uveitis / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D000975:Antioxidants; D000511:alpha-Macroglobulins; D013482:Superoxide Dismutase; D008550:Melatonin
keywords: aqueous humor; melatonin; tears; uveitis