title: Altered virulence potential of Salmonella Enteritidis cultured in different foods: A cumulative effect of differential gene expression and immunomodulation.
abstract: Salmonella enterica serovars Enteritidis (S. Enteritidis) is one of the most common causes of food borne illness. Bacterial growth environment plays an important role in regulating gene expression thereby affecting the virulence profile of the bacteria. Different foods present diverse growth conditions which may affect the pathogenic potential of the bacteria. In the present study, the effect of food environments on the pathogenic potential of S. Enteritidis has been evaluated. S. Enteritidis was grown in different foods e.g. egg white, peanut butter and milk, and virulent phenotypes were compared to those grown in Luria Bertani broth. In-vivo experiments in C57BL/6 mice revealed S. Enteritidis grown in egg white did not induce significant (p<0.001) production of proinflammatory cytokines in mice and were unable to cause colitis despite efficient colonization in cecum, mesenteric lymph node, spleen and liver. Further studies revealed that bacteria grown in LB activated MAP Kinase and NFκB pathways efficiently, while those grown in egg white poorly activated the above pathways which can account for the decreased production of proinflammatory cytokines. qRT PCR analysis revealed SPI-1 effectors were downregulated in bacteria grown in egg white. Interestingly, bacteria grown in egg white showed reversal of phenotype upon change in growth media to LB. Additionally, bacteria grown in milk and peanut butter showed different degrees of virulence in mice as compared to those grown in LB media. Thus, the present study demonstrates that, S. Enteritidis grown in egg white colonizes systemic sites without causing colitis in a mouse model, while bacteria grown in milk and peanut butter show different pathogenicity profiles suggesting that food environments significantly affect the pathogenicity of S. Enteritidis.
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, 751024, Odisha, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Jaiswal
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, 751024, Odisha, India.
authors.forename: Prakash Kumar
authors.initials: PK
authors.lastname: Sahoo
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, 751024, Odisha, India.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ryan
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, 751024, Odisha, India.
authors.forename: Jugal Kishore
authors.initials: JK
authors.lastname: Das
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, 751024, Odisha, India.
authors.forename: Eesha
authors.initials: E
authors.lastname: Chakraborty
authors.affiliation: Kalinga Institute of Medical Sciences, KIIT University, Bhubaneswar, 751024, Odisha, India.
authors.forename: Nirmal Kumar
authors.initials: NK
authors.lastname: Mohakud
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, 751024, Odisha, India. Electronic address: msbiotek@yahoo.com.
authors.forename: Mrutyunjay
authors.initials: M
authors.lastname: Suar
mesh_terms: D000818:Animals; D010367:Arachis / Q000382:microbiology*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D003092:Colitis / Q000382:microbiology*; D004529:Egg White / Q000382:microbiology*; D005517:Foodborne Diseases / Q000382:microbiology*; D056747:Immunomodulation; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk / Q000382:microbiology*; D010455:Peptides / Q000378:metabolism; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D037521:Virulence Factors; C086597:lambda Spi-1
keywords: Colitis; Egg white; Growth environment; S. Enteritidis; Virulence potential