title: Comparison of methods to improve induction of spermiation in wild-caught carp (Cyprinus carpio carpio), a threatened species from the Caspian Sea basin.
abstract: Wild carp (Cyprinus carpio carpio) forms the basis of an important fishery in the Southern Caspian Sea Basin which is increasingly underpinned by the release of cultured juveniles. A significant bottleneck to hatchery-rearing of juveniles is the spermiation of male broodstock. Therefore, four approaches to improving spermiation were investigated. The effectiveness of two delivery methods for the sustained release of salmon gonadotropin releasing hormone analogue (sGnRHa; i.e., via intramuscular cholesterol pellet vs emulsion injection) on the spermiation success and duration, sperm quality and quantity over 14days in wild-caught carp were compared to single injection of sGnRHa with Pimozide(®) (Linpe method) or carp pituitary extract (CPE). The consequence of the spermiation treatments on resulting embryonic quality was evaluated for subsequent fertilization and hatching success from wild male carp (mean weight±S.D. 1021±112g). All hormonal treatments, except for Linpe method, led to 100% spermiation of treated fish compared to only 25% in the control with no hormone intervention. The duration of spermiation, as well as the various quantitative variables of the sperm and the mean total sperm production were all generally improved with the sustained hormone delivery compared with the acute treatments. The GnRHa-FIA was the most effective method for improving spermiation.
authors.affiliation: Department of Natural Resources and Environmental Engineering, School of Agriculture, Shiraz University, Shiraz, Iran. Electronic address: vazirzadeh@shirazu.ac.ir.
authors.forename: Arya
authors.initials: A
authors.lastname: Vazirzadeh
authors.affiliation: Institute of Marine Science, University of Auckland, Auckland, New Zealand.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Farhadi
authors.affiliation: Department of Natural Resources and Environmental Engineering, School of Agriculture, Shiraz University, Shiraz, Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Naseri
authors.affiliation: Institute of Marine Science, University of Auckland, Auckland, New Zealand.
authors.forename: Andrew
authors.initials: A
authors.lastname: Jeffs
mesh_terms: D000818:Animals; D002347:Carps / Q000502:physiology*; D004343:Drug Implants; D056727:Endangered Species; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D009792:Oceans and Seas; D010868:Pimozide / Q000008:administration & dosage / Q000494:pharmacology*; D010902:Pituitary Gland; D013045:Species Specificity; D013091:Spermatogenesis / Q000187:drug effects*; D014020:Tissue Extracts / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D004343:Drug Implants; D014020:Tissue Extracts; D010868:Pimozide; D007987:Gonadotropin-Releasing Hormone; C058987:5-oxo-pro-his-trp-ser-tyr-gly-trp-leu-pro-glynh2
keywords: Cholesterol pellet; Cyprinus carpio carpio.; Emulsified GnRHa; Sperm quantity and quality; Spermiation; Sustained releasing delivery methods