title: Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces.
abstract: Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1ω9, C18 : 1ω9 aldehyde, C16 : 0 and C16 : 1ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4-56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae, for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T).
authors.affiliation: Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Andrei N
authors.initials: AN
authors.lastname: Shkoporov
authors.affiliation: Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Chaplin
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Shcherbakova
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors.forename: Natalia E
authors.initials: NE
authors.lastname: Suzina
authors.affiliation: Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Lyudmila I
authors.initials: LI
authors.lastname: Kafarskaia
authors.affiliation: Department of Molecular Biology and Experimental Tumor Therapies, Russian Scientific Center of Roentgenoradiology, Moscow 117997, Russia.
authors.forename: Vladimir K
authors.initials: VK
authors.lastname: Bozhenko
authors.affiliation: Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: Boris A
authors.initials: BA
authors.lastname: Efimov
mesh_terms: D000328:Adult; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002675:Child, Preschool; D000068016:Eubacteriales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000235:genetics; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis*; D008297:Male; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid