title: Quantifying Aflatoxin B1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles.
abstract: Rare earth doped upconversion nanoparticles convert near-infrared excitation light into visible emission light. Compared to organic fluorophores and semiconducting nanoparticles, upconversion nanoparticles (UCNPs) offer high photochemical stability, sharp emission bandwidths, and large anti-Stokes shifts. Along with the significant light penetration depth and the absence of autofluorescence in biological samples under infrared excitation, these UCNPs have attracted more and more attention on toxin detection and biological labelling. Herein, the fluorescence probe based on UCNPs was developed for quantifying Aflatoxin B1 (AFB1) in peanut oil. Based on a specific immunity format, the detection limit for AFB1 under optimal conditions was obtained as low as 0.2ng·ml(-1), and in the effective detection range 0.2 to 100ng·ml(-1), good relationship between fluorescence intensity and AFB1 concentration was achieved under the linear ratios up to 0.90. Moreover, to check the feasibility of these probes on AFB1 measurements in peanut oil, recovery tests have been carried out. A good accuracy rating (93.8%) was obtained in this study. Results showed that the nanoparticles can be successfully applied for sensing AFB1 in peanut oil.
authors.affiliation: School of Food and Biological engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Sun
authors.affiliation: School of Food and Biological engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute for Global Food Security, Queen's University Belfast, BT95GN, Northern Ireland, United Kingdom.
authors.forename: Anastasios
authors.initials: A
authors.lastname: Koidis
authors.affiliation: School of Food and Biological engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: qschen@ujs.edu.cn.
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Chen
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D055910:Antibodies, Immobilized / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008674:Metals, Rare Earth / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D000074241:Peanut Oil; D010938:Plant Oils / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D055910:Antibodies, Immobilized; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D008674:Metals, Rare Earth; D000074241:Peanut Oil; D010938:Plant Oils; D016604:Aflatoxin B1
keywords: Biological labelling; Fluorescence probes; Rapid toxin detection; Upconversion nanoparticles