title: Botrytis pyriformis sp. nov., a novel and likely saprophytic species of Botrytis.
abstract: A novel species of Botrytis from Sedum sarmentosum was described based on morphology and analyses of DNA sequences of nuc rDNA ITS regions and three nuclear genes (G3PDH, HSP60, RPB2). Meanwhile pathogenicity in 32 plant species, response to temperature for growth and conidial germination for the species were determined. The Botrytis species was named Botrytis pyriformis sp. nov. It was characterized by formation of grayish mycelia, brownish conidia and melanized sclerotia on PDA. The conidia are pear-shaped, melanized and covered with abundant villiform appendages on the conidial surface. Comparison of the ITS sequences confirmed its placement in the genus Botrytis Phylogenetic analysis based on DNA sequences of G3PDH, HSP60 and RPB2 genes indicated that B. pyriformis and other 30 Botrytis species form a monophyletic clade, which was further divided into three subclades. Subclade I comprised B. pyriformis alone, whereas subclades II and III comprised six and 24 Botrytis species, respectively. Botrytis pyriformis could not infect 32 plant species including S. sarmentosum, possibly due to deficiency in formation of infection cushions. This study presents a formal description and illustrations for B. pyriformis and provides experimental evidence, indicating that B. pyriformis might be a saprophytic species.
authors.affiliation: State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Q Y
authors.initials: QY
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: M D
authors.initials: MD
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Zhuang
authors.affiliation: USDA Agricultural Research Service, Washington State University, Pullman, Washington.
authors.forename: W D
authors.initials: WD
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China guoqingli@mail.hzau.edu.cn.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Li
mesh_terms: D020171:Botrytis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D018834:Chaperonin 60 / Q000235:genetics; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D031963:Sedum / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D018834:Chaperonin 60; D004271:DNA, Fungal; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D012319:RNA Polymerase II
keywords: Botrytis pyriformis; Sedum sarmentosum; molecular identification; saprophyte