title: A next-generation proteome array for Schistosoma mansoni.
abstract: A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil; Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Rafael Ramiro
authors.initials: RR
authors.lastname: de Assis
authors.affiliation: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ludolf
authors.affiliation: Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Rie
authors.initials: R
authors.lastname: Nakajima
authors.affiliation: Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Al
authors.initials: A
authors.lastname: Jasinskas
authors.affiliation: Vale Technology Institute of Sustainable Development, Belém, PA, Brazil.
authors.forename: Guilherme C
authors.initials: GC
authors.lastname: Oliveira
authors.affiliation: Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil.
authors.forename: Soraya T
authors.initials: ST
authors.lastname: Gaze
authors.affiliation: Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health & Medicine, James Cook University, Cairns, QLD, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Loukas
authors.affiliation: Departments of Biochemistry and Pathology, School of Medicine, University of Texas Health Science Center, 7703 Floyd Curl Dr., San Antonio, TX 78229, USA.
authors.forename: Philip T
authors.initials: PT
authors.lastname: LoVerde
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil; Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Science, The George Washington University, Washington, DC, USA. Electronic address: jbethony@gwu.edu.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Bethony
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Correa-Oliveira
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Instituto Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil; Protein Microarray Laboratory, Division of Infectious Disease, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Calzavara-Silva
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D019295:Computational Biology; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D010865:Pilot Projects; D040081:Protein Array Analysis / Q000379:methods; D020543:Proteome / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D008565:Membrane Proteins; D020543:Proteome
keywords: Antibody signature; Protein microarray; Proteome; Schistosoma mansoni