title: Activation of chicken bone marrow-derived dendritic cells induced by a Salmonella Enteritidis ghost vaccine candidate.
abstract: Bacterial Ghost-based vaccine development has been applied to a variety of gram-negative bacteria. Developed Salmonella Enteritidis (S. Enteritidis) ghost are promising vaccine candidates because of their immunogenic and enhanced biosafety potential. In this study, we aimed to evaluate the immunostimulatory effect of a S. Enteritidis ghost vaccine on the maturation of chicken bone marrow-derived dendritic cells (chBM-DCs) in vitro The immature chBM-DCs were stimulated with S. Enteritidis ghost vaccine candidate. The vaccine efficiently stimulated maturation events in chBM-DCs, indicated by up-regulated expression of CD40, CD80, and MHC-II molecules. Immature BM-DCs responded to stimulation with S. Enteritidis ghost by increased expression of IL-6 and IL-12p40 cytokines. Also, S. Enteritidis ghost stimulated chBM-DCs induced the significant expression of IFN-γ and IL-2 in co-cultured autologous CD4+ T cells. In conclusion, our data suggest that S. Enteritidis ghost vaccine candidate is capable of activating and interacting with chBM-DCs. The results from current study may help for rational designing of Salmonella ghost based heterologous antigen delivery platforms to dendritic cells.
authors.affiliation: Department of Bioactive Material Sciences and Department of Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 570-752 Republic of Korea.
authors.forename: N M
authors.initials: NM
authors.lastname: Kamble
authors.affiliation: Department of Bioactive Material Sciences and Department of Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 570-752 Republic of Korea.
authors.forename: C V
authors.initials: CV
authors.lastname: Jawale
authors.affiliation: Department of Bioactive Material Sciences and Department of Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 570-752 Republic of Korea johnhlee@jbnu.ac.kr.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology / Q000382:microbiology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002645:Chickens; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology; D005260:Female; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms
chemical_list: D030161:Avian Proteins; D022562:Salmonella Vaccines
keywords: Salmonella Enteritidis; chicken dendritic cell; cytokine; ghost vaccine; immune response