title: Dynamics of microbial community composition and soil organic carbon mineralization in soil following addition of pyrogenic and fresh organic matter.
abstract: Pyrogenic organic matter (PyOM) additions to soils can have large impacts on soil organic carbon (SOC) cycling. As the soil microbial community drives SOC fluxes, understanding how PyOM additions affect soil microbes is essential to understanding how PyOM affects SOC. We studied SOC dynamics and surveyed soil bacterial communities after OM additions in a field experiment. We produced and mixed in either 350 °C corn stover PyOM or an equivalent initial amount of dried corn stover to a Typic Fragiudept soil. Stover increased SOC-derived and total CO2fluxes (up to 6x), and caused rapid and persistent changes in bacterial community composition over 82 days. In contrast, PyOM only temporarily increased total soil CO2fluxes (up to 2x) and caused fewer changes in bacterial community composition. Of the operational taxonomic units (OTUs) that increased in response to PyOM additions, 70% also responded to stover additions. These OTUs likely thrive on easily mineralizable carbon (C) that is found both in stover and, to a lesser extent, in PyOM. In contrast, we also identified unique PyOM responders, which may respond to substrates such as polyaromatic C. In particular, members of Gemmatimonadetes tended to increase in relative abundance in response to PyOM but not to fresh organic matter. We identify taxa to target for future investigations of the mechanistic underpinnings of ecological phenomena associated with PyOM additions to soil.
authors.affiliation: School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.|Department of Soil Science, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Thea
authors.initials: T
authors.lastname: Whitman
authors.affiliation: School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Pepe-Ranney
authors.affiliation: School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
authors.forename: Akio
authors.initials: A
authors.lastname: Enders
authors.affiliation: School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
authors.forename: Chantal
authors.initials: C
authors.lastname: Koechli
authors.affiliation: School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Campbell
authors.affiliation: School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Buckley
authors.affiliation: School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.|Atkinson Center for a Sustainable Future, Cornell University, Ithaca, NY, USA.
authors.forename: Johannes
authors.initials: J
authors.lastname: Lehmann
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000032:analysis / Q000378:metabolism; D057486:Carbon Cycle; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D012987:Soil; D002244:Carbon