title: EphB6 overexpression and Apc mutation together promote colorectal cancer.
abstract: The erythropoietin-producing hepatocyte (Eph) family tyrosine kinases play important roles in tumorigenesis and cancer aggression. In this study, we investigated the role of EphB6 in oncogenic transformation of colorectal epithelial cells in vitro and in vivo. EphB6 is upregulated in human colorectal cancer (CRC) tissues as compared to normal tissues, and its overexpression promotes proliferation, migration and invasion by IMCE colorectal adenoma cells, in which one Apc allele is mutated. EphB6 overexpression together with Apc mutation leads to the development of colorectal tumors in vivo. Expression microarrays using mRNAs and lncRNAs isolated from EphB6-overexpresssing IMCE and control cells revealed a large number of dysregulated genes involved in cancer-related functions and pathways. The present study is the first to demonstrate that EphB6 overexpression together with Apc gene mutations may enhance proliferation, invasion and metastasis by colorectal epithelial cells. Microarray data and pathway analysis of differentially expressed genes provided insight into possible EphB6-regulated mechanisms promoting tumorigenesis and cancer progression. EphB6 overexpression may represent a novel, effective biomarker predictive of cell proliferation, invasion and metastasis patterns in CRC tumors.
authors.affiliation: Digestive System Department, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Mingqi
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Fubin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xin Yue
authors.initials: XY
authors.lastname: Gu
authors.affiliation: Department of Surgery, The Second Affiliated of Harbin Medical University, Harbin, China.
authors.forename: Dongwei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Digestive System Department, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Youlin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Cui
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Jinxue
authors.initials: J
authors.lastname: Tong
authors.affiliation: Division of Hematology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D017491:Genes, APC; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009154:Mutation; D051381:Rats; D036081:Receptors, Eph Family / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction; D014162:Transfection
chemical_list: C106797:EPHB6 protein, human; D036081:Receptors, Eph Family
keywords: APC; EphB6; biomarker; colorectal cancer; pathway analysis