title: Synergistic effect of chelators and Herbaspirillum sp. GW103 on lead phytoextraction and its induced oxidative stress in Zea mays.
abstract: Phytoremediation is an in situ, low-cost strategy for cleanup of the sites contaminated with heavy metals. Experiments were conducted to assess the impact of synthetic chelators and plant growth-promoting rhizosphere bacteria (Herbaspirillum sp. GW103) on heavy metal lead (Pb) uptake in Z. mays cultivated in Pb-contaminated soil. The present study investigated the Pb phytoaccumulation rate and plant antioxidant enzyme activities in Z. mays exposed to 100 mg/kg of PbNO3. The combination of gluconic acid (GA) with Herbaspirillum sp. GW103 treatment showed higher Pb solubility (18.9 mg/kg) compared with other chelators. The chemical chelators showed the significant difference in phytoaccumulation as well as antioxidant enzyme activities. The antioxidant enzymes such as catalase, peroxidase and superoxide dismutase activities changed under Pb stress. The study indicated that increased activity of antioxidant enzymes may play as signal inducers to fight against Pb.
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570 752, South Korea. gova.muthu@gmail.com.
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Govarthanan
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570 752, South Korea.
authors.forename: Seralathan
authors.initials: S
authors.lastname: Kamala-Kannan
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570 752, South Korea.
authors.forename: Seol Ah
authors.initials: SA
authors.lastname: Kim
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570 752, South Korea.
authors.forename: Young-Seok
authors.initials: YS
authors.lastname: Seo
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570 752, South Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Park
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570 752, South Korea. btoh@jbnu.ac.kr.
authors.forename: Byung-Taek
authors.initials: BT
authors.lastname: Oh
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002374:Catalase / Q000378:metabolism; D002614:Chelating Agents / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism; D042803:Herbaspirillum / Q000201:enzymology / Q000378:metabolism*; D007854:Lead / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D010544:Peroxidases / Q000378:metabolism; D058441:Rhizosphere; D012989:Soil Pollutants / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism* / Q000382:microbiology
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D005942:Gluconates; D009566:Nitrates; D012989:Soil Pollutants; D007854:Lead; C017461:lead nitrate; D010544:Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; C030691:gluconic acid
keywords: Antioxidant; Bioremediation; Chelators; Metals; Stress