title: Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS).
abstract: The major structural components of the nuclear lamina are the A- and B-type nuclear lamin proteins which are also present in the nucleoplasm. Studies of molecular movements of the lamins in both the lamina and nucleoplasm of living cell nuclei have provided insights into their roles in maintaining nuclear architecture. In this chapter, we present protocols for quantitatively measuring the mobilities of lamin proteins by fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) in mammalian cell nuclei.
authors.affiliation: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Chicago Ave., Chicago, IL, 60611, USA.|Department of Human Genetics, University of Chicago, Chicago, IL, USA.
authors.forename: Shimi
authors.initials: S
authors.lastname: Takeshi
authors.affiliation: ASAN Institute for Life Sciences, ASAN Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, South Korea.
authors.forename: Chan-Gi
authors.initials: CG
authors.lastname: Pack
authors.affiliation: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Chicago Ave., Chicago, IL, 60611, USA. r-goldman@northwestern.edu.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Goldman
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D015870:Gene Expression; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D034882:Lamins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D013050:Spectrometry, Fluorescence / Q000379:methods; D014162:Transfection
chemical_list: D034882:Lamins; D011993:Recombinant Fusion Proteins
keywords: FCS; FRAP; Lamin; Lamina