title: Suppression of rolling circle amplification by nucleotide analogs in circular template for three DNA polymerases.
abstract: Among wide applications of nucleotide analogs, their roles in enzyme catalytic reactions are significant in both fundamental and medical researches. By introducing analogs into circular templates, we succeeded in determining effects of four analogs on RCA efficiency for three different DNA polymerases. Results showed an obvious suppression effect for 2'-OMeRNA modification, which might be due to the size of the C2'-modified moieties. 2'-F RNA, LNA and PS had little interference, suggesting good analog candidates for application in RCA. Different polymerases and nucleobases made a little difference according to analogs we used. These results are useful for understanding polymerase catalytic mechanism and analogs applications in RCA reaction.
authors.affiliation: a Department of Biochemistry and Molecular Biology , China Medical University , Shenyang , China.
authors.forename: Suming
authors.initials: S
authors.lastname: Tang
authors.affiliation: a Department of Biochemistry and Molecular Biology , China Medical University , Shenyang , China.|b Department of Aquaculture , Animal Science and Veterinary Medicine College, Shenyang Agricultural University , Shenyang , China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: a Department of Biochemistry and Molecular Biology , China Medical University , Shenyang , China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Hu
authors.affiliation: a Department of Biochemistry and Molecular Biology , China Medical University , Shenyang , China.
authors.forename: Jiquan
authors.initials: J
authors.lastname: Jiang
authors.affiliation: a Department of Biochemistry and Molecular Biology , China Medical University , Shenyang , China.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Chang
authors.affiliation: a Department of Biochemistry and Molecular Biology , China Medical University , Shenyang , China.
authors.forename: Yifu
authors.initials: Y
authors.lastname: Guan
authors.affiliation: a Department of Biochemistry and Molecular Biology , China Medical University , Shenyang , China.
authors.forename: Guojie
authors.initials: G
authors.lastname: Zhao
mesh_terms: D017103:Bacillus Phages / Q000737:chemistry / Q000201:enzymology; D011088:DNA Ligases / Q000737:chemistry / Q000378:metabolism; D004270:DNA, Circular / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D054735:Phosphorothioate Oligonucleotides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D054735:Phosphorothioate Oligonucleotides; C477371:locked nucleic acid; D004259:DNA-Directed DNA Polymerase; D011088:DNA Ligases; C120640:DNA ligase (NAD)
keywords: 2′-F RNA; 2′-OMeRNA; LNA; nucleotide analogs; rolling circle amplification