title: Impact of exogenous GABA treatments on endogenous GABA metabolism in anthurium cut flowers in response to postharvest chilling temperature.
abstract: Anthurium flowers are susceptible to chilling injury, and the optimum storage temperature is 12.5-20 °C. The γ-aminobutyric acid (GABA) shunt pathway may alleviate chilling stress in horticultural commodities by providing energy (ATP), reducing molecules (NADH), and minimizing accumulation of reactive oxygen species (ROS). In this experiment, the impact of a preharvest spray treatment with 1 mM GABA and postharvest treatment of 5 mM GABA stem-end dipping on GABA shunt pathway activity of anthurium cut flowers (cv. Sirion) in response to cold storage (4 °C for 21 days) was investigated. GABA treatments resulted in lower glutamate decarboxylase (GAD) and higher GABA transaminase (GABA-T) activities in flowers during cold storage, which was associated with lower GABA content and coincided with higher ATP content. GABA treatments also enhanced accumulation of endogenous glycine betaine (GB) in flowers during cold storage, as well as higher spathe relative water content (RWC). These findings suggest that GABA treatments may alleviate chilling injury of anthurium cut flowers by enhancing GABA shunt pathway activity leading to provide sufficient ATP and promoting endogenous GB accumulation.
authors.affiliation: Department of Horticultural Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Electronic address: aghdamm@ut.ac.ir.
authors.forename: Morteza Soleimani
authors.initials: MS
authors.lastname: Aghdam
authors.affiliation: Department of Horticultural Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Roohangiz
authors.initials: R
authors.lastname: Naderi
authors.affiliation: Department of Agricultural Biotechnology, Imam Khomeini International University, Qazvin, Iran.
authors.forename: Abbasali
authors.initials: A
authors.lastname: Jannatizadeh
authors.affiliation: Department of Horticultural Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mesbah
authors.initials: M
authors.lastname: Babalar
authors.affiliation: Department of Horticultural Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Mohammad Ali Askari
authors.initials: MA
authors.lastname: Sarcheshmeh
authors.affiliation: Department of Agronomy and Plant Breeding, University of Tehran, Karaj, 31587-77871, Iran.
authors.forename: Mojtaba Zamani
authors.initials: MZ
authors.lastname: Faradonbe
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000378:metabolism; D029064:Araceae / Q000187:drug effects / Q000502:physiology*; D001622:Betaine / Q000378:metabolism; D003080:Cold Temperature; D035264:Flowers / Q000187:drug effects / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D014867:Water / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D014867:Water; D001622:Betaine; D005680:gamma-Aminobutyric Acid; D000612:4-Aminobutyrate Transaminase; D005968:Glutamate Decarboxylase
keywords: Anthurium cut flowers; Chilling injury; GABA transaminase; Glycine betaine; Postharvest; γ-Aminobutyric acid