title: Induction of Lipocalin2 in a Rat Model of Lung Irradiation.
abstract: Previously, we showed that lipocalin2 (LCN2) serum levels increased after liver irradiation and during acute-phase conditions. Here, we evaluate LCN2 expression and serum levels after single-dose lung irradiation with 25 Gy, percutaneously administered to the lung of randomly-paired male Wistar rats. Due to the concave anatomy of the lung recesses, the irradiation field included the upper part of the liver. No rat died due to irradiation. In control tissue, lung immunohistochemistry showed a high constitutive expression of LCN2+ granulocytes. LCN2 mRNA levels in lung tissue increased up to 24 h (9 ± 2.3-fold) after irradiation. However, serum LCN2 levels remained undetectable after lung irradiation. LCN2 expression in the upper part of the liver increased up to 4.2-fold after lung irradiation, but the lower liver showed an early decrease. Acute-phase cytokines (IL-1β and TNF-α) showed a significant increase on transcript level in both lung and upper liver, whilst the lower liver did not show any considerable increase. In conclusion, constitutive expression of LCN2 in local immune cells demonstrates its local role during stress conditions in the lung. The absence of LCN2 in the serum strengthens our previous findings that the liver is the key player in secreting LCN2 during stress conditions with liver involvement.
authors.affiliation: Clinic for Gastroenterology and Endocrinology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. drsadafsultan@gmail.com.|Clinic for Radiotherapy and Radiation Oncology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. drsadafsultan@gmail.com.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Sultan
authors.affiliation: Clinic for Gastroenterology and Endocrinology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. shakil.ahmad@med.uni-goettingen.de.
authors.forename: Shakil
authors.initials: S
authors.lastname: Ahmad
authors.affiliation: Clinic for Radiotherapy and Radiation Oncology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. mfraenk@med.uni-goettingen.de.
authors.forename: Margret
authors.initials: M
authors.lastname: Rave-Fränk
authors.affiliation: Clinic for Gastroenterology and Endocrinology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. i.malik@med.uni-goettingen.de.
authors.forename: Ihtzaz Ahmed
authors.initials: IA
authors.lastname: Malik
authors.affiliation: Clinic for Radiotherapy and Radiation Oncology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. cfhess@med.uni-goettingen.de.
authors.forename: Clemens F
authors.initials: CF
authors.lastname: Hess
authors.affiliation: Clinic for Radiotherapy and Radiation Oncology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. Christiansen.Hans@mh-hannover.de.
authors.forename: Hans
authors.initials: H
authors.lastname: Christiansen
authors.affiliation: Clinic for Gastroenterology and Endocrinology, University Medical Center Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. silke.cameron@med.uni-goettingen.de.
authors.forename: Silke
authors.initials: S
authors.lastname: Cameron
mesh_terms: D000818:Animals; D005720:Gamma Rays; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000378:metabolism; D000071068:Lipocalin-2 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; C097278:Lcn2 protein, rat; D000071068:Lipocalin-2; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
keywords: lipocalin2; lung irradiation; upper and lower part of the liver