title: Influence of bronchoalveolar lavage volume on cytological profiles  and subsequent diagnosis of inflammatory airway disease in horses.
abstract: The aim of the study was to determine whether instillation of either 250 mL or 500 mL of saline for bronchoalveolar lavage (BAL) would influence cytological confirmation of inflammatory airway disease (IAD). Thirty client-owned Standardbred racehorses were sampled via endoscopy with 250 mL of saline in one lung and 500 mL in the contralateral lung. The procedure was repeated 72 h later, reversing the volume per lung. The proportions of BAL fluid (BALF) recovered were significantly higher and neutrophil percentages significantly lower with the larger volume. A poor agreement was found between methodologies in terms of final diagnosis, when based on proportions of neutrophils (>10% from at least one lung). Within the recommended range (250–500 mL), the instilled volume significantly influenced cytological profiles. Establishing specific BALF reference values is warranted.
authors.forename: Marie
authors.initials: M
authors.lastname: Orard
authors.forename: Marianne
authors.initials: M
authors.lastname: Depecker
authors.forename: Erika
authors.initials: E
authors.lastname: Hue
authors.forename: Pierre-Hugues
authors.initials: PH
authors.lastname: Pitel
authors.forename: Anne
authors.initials: A
authors.lastname: Couroucé-Malblanc
authors.forename: Eric A
authors.initials: EA
authors.lastname: Richard
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage / Q000662:veterinary*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D012120:Respiration Disorders / Q000175:diagnosis / Q000662:veterinary*; D012965:Sodium Chloride / Q000008:administration & dosage
chemical_list: D012965:Sodium Chloride