title: A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.
abstract: BACKGROUND
  Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses.
  
  
  RESULTS
  Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant.
  
  
  CONCLUSIONS
  The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.
authors.forename: Paula Beatriz
authors.initials: PB
authors.lastname: Santiago
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.|Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, Rockville, Maryland, United States of America.
authors.forename: Teresa C F
authors.initials: TC
authors.lastname: Assumpção
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.|Ceilândia Faculty, The University of Brasília, Brasília, Brazil.
authors.forename: Carla Nunes
authors.initials: CN
authors.lastname: de Araújo
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.
authors.forename: Izabela Marques Dourado
authors.initials: IM
authors.lastname: Bastos
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.
authors.forename: David
authors.initials: D
authors.lastname: Neves
authors.affiliation: Department of Parasitology, The University of Goiás, Jataí, Brazil.
authors.forename: Ionizete Garcia
authors.initials: IG
authors.lastname: da Silva
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Charneau
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.
authors.forename: Rayner Myr L
authors.initials: RM
authors.lastname: Queiroz
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.|Instituto Leônidas e Maria Deane - Fiocruz Amazônia, Manaus, Brazil.
authors.forename: Tainá
authors.initials: T
authors.lastname: Raiol
authors.affiliation: Department of Computer Science, The University of Brasília, Brasília, Brazil.
authors.forename: João Victor de Araújo
authors.initials: JV
authors.lastname: Oliveira
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.
authors.forename: Marcelo Valle
authors.initials: MV
authors.lastname: de Sousa
authors.affiliation: Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, Rockville, Maryland, United States of America.
authors.forename: Eric
authors.initials: E
authors.lastname: Calvo
authors.affiliation: Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, Rockville, Maryland, United States of America.
authors.forename: José M C
authors.initials: JM
authors.lastname: Ribeiro
authors.affiliation: Department of Cell Biology, The University of Brasília, Brasília, Brazil.
authors.forename: Jaime M
authors.initials: JM
authors.lastname: Santana
mesh_terms: D000818:Animals; D023281:Genomics; D007303:Insect Vectors; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000032:analysis* / Q000235:genetics*; D040901:Proteomics; D012239:Rhodnius / Q000502:physiology*; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012471:Salivary Proteins and Peptides; D010447:Peptide Hydrolases