title: The epithelial-mesenchymal transition phenotype of metastatic lymph nodes impacts the prognosis of esophageal squamous cell carcinoma patients.
abstract: Epithelial-mesenchymal transition (EMT) plays a key role in tumor metastasis, but the significance of EMT phenotype to the prognosis of esophageal squamous cell carcinoma (ESCC) patients remains unclear. We used immunohistochemistry to examine the expression of the EMT-related proteins E-cadherin, N-cadherin and vimentin in samples of T3N1-3M0 ESCC from 155 primary tumors (PTs) with paired metastatic lymph nodes (MLNs) and 58 PTs without paired MLNs. Based on the expression pattern of the EMT markers, PTs and MLNs were classified as EMT wild, hybrid, null or complete type. The hybrid (42.7%) and complete (39.4%) types predominated among PTs, whereas the wild (34.2%) and hybrid (52.9%) types predominated among MLNs, and EMT phenotypes differed between the paired PTs and MLNs (P < 0.001). Univariate analysis revealed that, for PTs, the EMT phenotype was associated with N-stage (P = 0.039) but not patient survival, and that patients with complete or hybrid type MLNs had better overall survival (OS, P = 0.001) and disease-free survival (DFS, P = 0.005) than patients with null and wild type MLNs, especially those with N1-stage disease (P = 0.017 for OS, and P = 0.017 for DFS, respectively). Multivariate analysis revealed that wild and null type MLNs as well as older age and N2-3 stage were independent predictors of OS and DFS (P < 0.05). Thus MLNs exhibit EMT phenotypes that are distinct from those of their PT and may serve as a novel independent prognostic indicator in ESCC.
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.|Guangdong Esophageal Cancer Institute, Guangzhou 510060, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.|Guangdong Esophageal Cancer Institute, Guangzhou 510060, China.|Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Kong-Jia
authors.initials: KJ
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.|Guangdong Esophageal Cancer Institute, Guangzhou 510060, China.|Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Qian-Wen
authors.initials: QW
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, Cancer Hospital of Shantou University Medical College, Shantou 515041, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Mei-Fang
authors.initials: MF
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.|Guangdong Esophageal Cancer Institute, Guangzhou 510060, China.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.|Guangdong Esophageal Cancer Institute, Guangzhou 510060, China.|Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.|Guangdong Esophageal Cancer Institute, Guangzhou 510060, China.|Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.|Guangdong Esophageal Cancer Institute, Guangzhou 510060, China.|Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D010641:Phenotype; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor
keywords: epithelial-mesenchymal transition; esophageal squamous cell carcinoma; metastatic lymph node; prognosis