title: Qualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD.
abstract: A high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) method in both positive and negative ion modes was established to investigate the major constituents in the ethanolic extract of Centipeda minima (EBSC). Twelve common components including flavones and their glycosides, phenolic and polyphenolic acids, and sesquiterpene lactone were identified in ten batches of samples based on comparison with the retention time and accurate mass of external standards (mass accuracy within 3ppm) or the fragmentation patterns of tandem MS. Meanwhile, a simple, accurate and reliable HPLC-DAD method was also developed to determine the content of 10 chemical markers simultaneously. Results obtained from method validations including linearity, accuracy and precision showed that this new method is reliable and robust. Isochlorogenic acid A and brevilin A were found to be the most abundant in the ethanol extract of EBSC and could be served as markers for quality control of EBSC.
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors.forename: Chi-On
authors.initials: CO
authors.lastname: Chan
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China.
authors.forename: Deng-Ping
authors.initials: DP
authors.lastname: Jin
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors.forename: Nai-Ping
authors.initials: NP
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China; Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address: sibao.chen@polyu.edu.hk.
authors.forename: Si-Bao
authors.initials: SB
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: daniel.mok@polyu.edu.hk.
authors.forename: Daniel Kam Wah
authors.initials: DK
authors.lastname: Mok
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D036301:Qualitative Research
keywords: Brevilin A; Centipeda minima; Herbal analysis; Isochlorogenic acid A; Quadrupole time-of-flight mass spectrometry (QTOF MS); Quality control