title: Analysis of HSP90 Expression Is Valuable in the Differential Diagnosis of Ocular Surface Squamous Lesions.
abstract: OBJECTIVE
  The aim of this study was to evaluate heat shock protein 90 (HSP90) expression in squamous lesions (SLs) and to assess its diagnostic value for different lesions within the SL spectrum.
  
  
  METHODS
  A total of 70 conjunctival SLs, including 19 papillomas, 22 cases of conjunctival intraepithelial neoplasia (ConINs) I, 11 cases of ConIN II, six cases of ConIN III, and 12 squamous carcinomas (sqCAs), were evaluated using the German immunoreactive score against HSP90.
  
  
  RESULTS
  Cytoplasmic HSP90 expression differed between low- and high-grade lesions (P < .001). Among high-grade lesions, the nuclear HSP90 score was higher in the ConIN III-sqCA group than in the ConIN II group (P = .0162). A percentage of total thickness staining of less than 73% differentiated between ConIN III and sqCA.
  
  
  CONCLUSIONS
  The expression of HSP90 is particularly useful to differentiate low-grade from high-grade lesions of the conjunctiva. HSP90 may play an important role in the malignant transformation of SLs and could be a new target for therapy.
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada; Pathology Department, School of Medicine, Pontifical Catholic University of Chile, Santiago. zoroquiain@gmail.com.
authors.forename: Pablo
authors.initials: P
authors.lastname: Zoroquiain
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Dana
authors.initials: D
authors.lastname: Faingold
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Sarah
authors.initials: S
authors.lastname: Algahmdi
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Natalia
authors.initials: N
authors.lastname: Vila
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Patrick
authors.initials: P
authors.lastname: Logan
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Debra-Meghan
authors.initials: DM
authors.lastname: Sanft
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Ana Beatriz
authors.initials: AB
authors.lastname: Toledo Dias
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Sultan
authors.initials: S
authors.lastname: Aldrees
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Vasco
authors.initials: V
authors.lastname: Bravo-Filho
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Julia
authors.initials: J
authors.lastname: Burnier
authors.affiliation: From the Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Canada;
authors.forename: Miguel N
authors.initials: MN
authors.lastname: Burnier
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002278:Carcinoma in Situ / Q000145:classification / Q000175:diagnosis*; D002294:Carcinoma, Squamous Cell / Q000145:classification / Q000175:diagnosis*; D016022:Case-Control Studies; D003228:Conjunctiva / Q000473:pathology; D003230:Conjunctival Neoplasms / Q000145:classification / Q000175:diagnosis*; D003593:Cytoplasm / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D010212:Papilloma / Q000145:classification / Q000175:diagnosis*; D011792:Quebec
chemical_list: D014408:Biomarkers, Tumor; D018841:HSP90 Heat-Shock Proteins
keywords: HSP90; Immunohistochemistry; Ocular surface squamous neoplasia