title: Critical Conditions for Adsorption of Calcium Ions onto Dipolar Lipid Membranes.
abstract: Dipolar lipid membranes may adsorb multivalent ions. The binding constant depends on the type of lipid and ions. In this paper, we focus on the adsorption of calcium ions onto 1,2-dilauroylphosphatidylcholine (DLPC) membrane. Using small-angle-X-ray scattering we found that at ambient room temperature ca. 0.6 mM CaCl2 is a critical concentration at which calcium ions adsorbed to 30 mg/mL (ca. 48 mM) DLPC membrane. We then determined the structure of the lamellar phases formed at CaCl2 concentrations below and above the critical concentration and characterized the effect of temperature and incubation time on the adsorption process. Our findings suggest that calcium adsorption to DLPC membranes requires an initial nucleation phase.
authors.affiliation: Institute of Chemistry, the Hebrew University of Jerusalem , Jerusalem 91904, Israel.
authors.forename: Oren
authors.initials: O
authors.lastname: Lotan
authors.affiliation: Institute of Chemistry, the Hebrew University of Jerusalem , Jerusalem 91904, Israel.
authors.forename: Lea
authors.initials: L
authors.lastname: Fink
authors.affiliation: Institute of Chemistry, the Hebrew University of Jerusalem , Jerusalem 91904, Israel.
authors.forename: Asaf
authors.initials: A
authors.lastname: Shemesh
authors.affiliation: Institute of Chemistry, the Hebrew University of Jerusalem , Jerusalem 91904, Israel.
authors.forename: Carmen
authors.initials: C
authors.lastname: Tamburu
authors.affiliation: Institute of Chemistry, the Hebrew University of Jerusalem , Jerusalem 91904, Israel.
authors.forename: Uri
authors.initials: U
authors.lastname: Raviv
mesh_terms: D000327:Adsorption; D002122:Calcium Chloride / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D010713:Phosphatidylcholines; C015153:1,2-dilauroylphosphatidylcholine; D002122:Calcium Chloride