title: Pseudomonas aeruginosa produces phosphatidyltris(hydroxymethyl)aminomethane and derivatives when grown in Tris-buffered medium.
abstract: For optimal growth of a microorganism, the pH of the culture medium should be set at an optimum value. For that reason, growth media require buffering agents. We show in this study that, when grown in a medium supplemented with tris(hydroxymethyl)aminomethane (Tris), Pseudomonas aeruginosa is able to use this organic compound to produce new phospholipids. We thus pointed out that phosphatidyltris(hydroxymethyl)aminomethane as well as diphosphatidyltris(hydroxymethyl)aminomethane was detected in membrane lipid extracts of bacteria grown in Tris-buffered medium. Moreover, the amounts of lysoglycerophospholipids in the lipidome of P. aeruginosa grown in Tris-buffered medium increased leading to the presence of lysophosphatidylglycerol and lysophosphatidyltris(hydroxymethyl)aminomethane as well as other lysophospholipid derivatives. Finally, we investigated the effect of the presence of these exogenous phospholipids on the susceptibility of P. aeruginosa to some antibiotics. We observed a decrease of the minimal inhibitory concentrations of different antibiotic families, i.e., fluoroquinolones, aminoglycosides, ß-lactams and polymyxins, proving the importance of the buffer choice for growth medium and its impact on the lipidome.
authors.affiliation: CNRS, UMR 6270, "Polymères, Biopolymères, Surfaces", Université de Rouen, F-76821 Mont-Saint-Aignan, France; Normandie Univ, F-14032 Caen, France. Electronic address: imen.abbes@etu.univ-rouen.fr.
authors.forename: Imen
authors.initials: I
authors.lastname: Abbes
authors.affiliation: CNRS, UMR 6270, "Polymères, Biopolymères, Surfaces", Université de Rouen, F-76821 Mont-Saint-Aignan, France; Normandie Univ, F-14032 Caen, France. Electronic address: christophe.rihouey@univ-rouen.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Rihouey
authors.affiliation: CNRS, UMR 6270, "Polymères, Biopolymères, Surfaces", Université de Rouen, F-76821 Mont-Saint-Aignan, France; Normandie Univ, F-14032 Caen, France; PISSARO Proteomics Facility, Université de Rouen, F-76821 Mont-Saint-Aignan, France. Electronic address: julie.hardouin@univ-rouen.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Hardouin
authors.affiliation: CNRS, UMR 6270, "Polymères, Biopolymères, Surfaces", Université de Rouen, F-76821 Mont-Saint-Aignan, France; Normandie Univ, F-14032 Caen, France. Electronic address: emmanuelle.de@univ-rouen.fr.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Dé
authors.affiliation: CNRS, UMR 6270, "Polymères, Biopolymères, Surfaces", Université de Rouen, F-76821 Mont-Saint-Aignan, France; Normandie Univ, F-14032 Caen, France; PISSARO Proteomics Facility, Université de Rouen, F-76821 Mont-Saint-Aignan, France. Electronic address: thierry.jouenne@univ-rouen.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.affiliation: CNRS, UMR 6270, "Polymères, Biopolymères, Surfaces", Université de Rouen, F-76821 Mont-Saint-Aignan, France; Normandie Univ, F-14032 Caen, France. Electronic address: stephane.alexandre@univ-rouen.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Alexandre
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D008744:Methylamines / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D014325:Tromethamine / Q000737:chemistry*
chemical_list: D003470:Culture Media; D008744:Methylamines; D014325:Tromethamine; C027451:methylamine
keywords: Antibiotics; Bacteria; Mass spectrometry; Phospholipid; Pseudomonas aeruginosa; Tris(hydroxymethyl)aminomethane