title: Comparative Transcriptomics Reveals Key Gene Expression Differences between Diapausing and Non-Diapausing Adults of Culex pipiens.
abstract: Diapause is a critical eco-physiological adaptation for winter survival in the West Nile Virus vector, Culex pipiens, but little is known about the molecular mechanisms that distinguish diapause from non-diapause in this important mosquito species. We used Illumina RNA-seq to simultaneously identify and quantify relative transcript levels in diapausing and non-diapausing adult females. Among 65,623,095 read pairs, we identified 41 genes with significantly different transcript abundances between these two groups. Transcriptome divergences between these two phenotypes include genes related to juvenile hormone synthesis, anaerobic metabolism, innate immunity and cold tolerance.
authors.affiliation: Department of Biology, Baylor University, Waco, TX, 76798, United States of America.
authors.forename: David S
authors.initials: DS
authors.lastname: Kang
authors.affiliation: Department of Biology, Baylor University, Waco, TX, 76798, United States of America.
authors.forename: Michael A
authors.identifier: 0000-0001-5200-1214
authors.initials: MA
authors.lastname: Cotten
authors.affiliation: Department of Evolution, Ecology, and Organismal Biology and Department of Entomology, Ohio State University, 318 West 12th Avenue, Columbus, OH, 43210, United States of America.
authors.forename: David L
authors.initials: DL
authors.lastname: Denlinger
authors.affiliation: Department of Biology, Baylor University, Waco, TX, 76798, United States of America.
authors.forename: Cheolho
authors.initials: C
authors.lastname: Sim
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000222:Adaptation, Physiological / Q000235:genetics; D000818:Animals; D003465:Culex / Q000235:genetics* / Q000502:physiology* / Q000821:virology; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D017344:Genes, Insect; D007303:Insect Vectors / Q000235:genetics* / Q000502:physiology* / Q000821:virology; D012621:Seasons; D014902:West Nile virus / Q000472:pathogenicity