title: [Effect of MicroRNA-146a on Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells].
abstract: OBJECTIVE
  To explore the effect of MicroRNA-146a (miR-146a) on the ability of BM-MSC to differentiate into adipocytes and osteoblasts.
  
  
  METHODS
  BM-MSC were isolated from the bone marrow of healthy donors. The differentiation of BM-MSC into adipocytes and osteoblasts cells were done in vitro. After transfection with miR-146a inhibitor or mimics, the expression of miR-146a in BM-MSC was detected by real time quantitative PCR. The effect of MicroRNA-146a on the differentiation potential of BM-MSC was evaluated after transfection.
  
  
  RESULTS
  BM-MSC possessed the ability to differentiate into adipocytes and osteoblasts cells when cultured in the induction medium. The expression of miR-146a was correspondingly down-regulated and up-regulated in BM-MSC after transfection. Compared with the control group, the expression of miR-146a was down-regulated (P < 0.01) after transfection with miR-146a inhibitor, while after transfection with miR-146a mimics it was significantly up-regulated. This study proved that the transfection with miR-146a inhibitor can inhibit BM-MSC differentiate into adipocytes (P < 0.01), while transfection with miR-146a mimics can promote differentiation of BM-MSC into adipocytes (P < 0.01). No effect of miR-146a inhibitor or miR-146a mimics on osteogenic differentiation of BM-MSC was observed (P > 0.05).
  
  
  CONCLUSIONS
  BM-MSC possess the ability to differentiate into adipocytes and osteoblasts. The miR-146a can promote BM-MSC to differentiate into adipocytes.
authors.affiliation: Department of Hematology of Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Hematology of Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China. E-mail: wangxingbing91@hotmail.com.
authors.forename: Xing-Bing
authors.initials: XB
authors.lastname: Wang
authors.affiliation: Department of Hematology of Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hematology of Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ming
mesh_terms: D017667:Adipocytes / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D035683:MicroRNAs / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D014162:Transfection
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs