title: Construction of a fucoidan/laminin functional multilayer to direction vascular cell fate and promotion hemocompatibility.
abstract: Surface biofunctional modification of cardiovascular stents is a versatile approach to reduce the adverse effects after implantation. In this work, a novel multifunctional coating was fabricated by coimmobilization of the sulfated polysaccharide of brown algae fucoidan and laminin to biomimic the vascular intimal conditions in order to support rapid endothelialization, prevent restenosis and improve hemocompatibility. The surface properties of the coating such as hydrophilicity, bonding density of biomolecules and stability were evaluated and optimized. According to the biocompatibility tests, the fucoidan/laminin multilayer coated surface displayed less platelet adhesion with favorable anticoagulant property. In addition, the fucoidan/laminin complex showed function to selectively regulate vascular cells growth behavior. The proliferation of endothelial cells (ECs) on the fucoidan/laminin biofunctional coating was significantly promoted. For the smooth muscle cells (SMCs), inhibitory effects on cell adhesion and proliferation were observed. In conclusion, the fucoidan/laminin biofunctional coating was successfully fabricated with desirable anticoagulant and endothelialization properties which show a promising application in the vascular devices such as vascular stents or grafts surface modification.
authors.affiliation: Key Laboratory of Advanced Materials Technology of Ministry of Education, Department of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Changrong
authors.initials: C
authors.lastname: Ye
authors.affiliation: Key Laboratory of Advanced Materials Technology of Ministry of Education, Department of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Advanced Materials Technology of Ministry of Education, Department of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Su
authors.affiliation: Key Laboratory of Advanced Materials Technology of Ministry of Education, Department of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Key Laboratory of Advanced Materials Technology of Ministry of Education, Department of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden 01069, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: F Maitz
authors.affiliation: Key Laboratory of Advanced Materials Technology of Ministry of Education, Department of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Anshan
authors.initials: A
authors.lastname: Zhao
mesh_terms: D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D007797:Laminin; D011134:Polysaccharides; C007789:fucoidan
keywords: Anticoagulation; Cardiovascular implants; Endothelial cells; Fucoidan; Laminin; Smooth muscle cells