title: Differential immunophenotype of macrophages in acute and chronic chorioamnionitis.
abstract: The aim of this study was to investigate the involvement and immunophenotype of macrophages in acute chorioamnionitis (ACA) and chronic chorioamnionitis (CCA), marking amniotic fluid infection and anti-fetal rejection, respectively.
  
  
  
  Chorioamniotic membranes from (1) gestational age-matched cases without chorioamnionitis, (2) cases with ACA, and (3) cases with CCA were studied after immunohistochemical staining using antibodies against CD14, CD68, CD163, and DC-SIGN.
  
  
  
  Macrophages increased prominently in the chorionic trophoblastic layer of both ACA and CCA cases in contrast to non-inflammatory cases. Macrophages in the decidua and the chorioamniotic membranes of ACA cases expressed CD14. Macrophages in the chorionic trophoblastic layer of CCA cases were characterized by CD68 positivity. DC-SIGN-positive cells were increased in the chorioamniotic mesodermal layer of CCA cases.
  
  
  
  Macrophages participate in the inflammatory response in ACA and CCA. The differential immunophenotypes of macrophages in the decidua and chorioamniotic membranes of ACA and CCA cases suggest their disease-specific and region-specific roles at the feto-maternal interface.
authors.affiliation: Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
authors.forename: Go-Eun
authors.initials: GE
authors.lastname: Bae
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Gyeonggi-do, Republic of Korea
authors.forename: Joon-Seok
authors.initials: JS
authors.lastname: Hong
authors.affiliation: Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea|Department of Health Sciences and Technology, Sungkyunkwan University, SAIHST, Seoul, Republic of Korea
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Park
authors.affiliation: Department of Health Sciences and Technology, Sungkyunkwan University, SAIHST, Seoul, Republic of Korea
authors.forename: Ja Yun
authors.initials: JY
authors.lastname: Jang
authors.affiliation: Department of Health Sciences and Technology, Sungkyunkwan University, SAIHST, Seoul, Republic of Korea
authors.forename: Yi Seul
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
authors.forename: Suk-Joo
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Oh
authors.affiliation: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
authors.forename: Cheong-Rae
authors.initials: CR
authors.lastname: Roh
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis; D002821:Chorioamnionitis / Q000276:immunology*; D002823:Chorion / Q000276:immunology; D003430:Cross-Sectional Studies; D003656:Decidua / Q000276:immunology; D005260:Female; D006801:Humans; D037181:Lectins, C-Type / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000032:analysis; D012189:Retrospective Studies; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
keywords: CD14; CD163; CD68; DC-SIGN; chorioamniotic membranes; decidua; inflammation; macrophages