title: Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation.
abstract: Cytokinesis is the final step in cell division that results in the separation of a parent cell into daughter cells. Unlike somatic cells that undergo symmetric division, meiotic division is highly asymmetric, allowing the preservation of maternal resources for embryo development. Beclin-1/BECN1, the mammalian homolog of yeast Atg6, is a key molecule of autophagy. As part of a class III phosphatidylinositol 3-kinase (PI3K-III) complex, BECN1 initiates autophagosome formation by coordinating membrane trafficking. However, emerging evidence suggests that BECN1 regulates chromosome segregation and cytokinesis during mitosis. Thus, we investigated the function of BECN1 during oocyte meiotic maturation. BECN1 was widely distributed during meiotic maturation forming small vesicles. Interestingly, BECN1 is also detected at the midbody ring during cytokinesis. Depletion of BECN1 impaired the cytokinetic abscission, perturbing the recruitment of ZFYVE26 at the midbody. Similar phenotypes were observed when PI3K-III activity was inhibited. However, inhibition of autophagy by depleting Atg14L did not disturb meiotic maturation. Therefore, our results not only demonstrate that BECN1 as a PI3K-III component is essential for cytokinesis, but also suggest that BECN1 is not associated with autophagy pathway in mouse oocytes.
authors.affiliation: a Department of Genetic Engineering , College of Biotechnology and Bioengineering, Sungkyunkwan University , Suwon , Gyeonggi-do , Korea.
authors.forename: Seung Yeop
authors.initials: SY
authors.lastname: You
authors.affiliation: a Department of Genetic Engineering , College of Biotechnology and Bioengineering, Sungkyunkwan University , Suwon , Gyeonggi-do , Korea.
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Park
authors.affiliation: a Department of Genetic Engineering , College of Biotechnology and Bioengineering, Sungkyunkwan University , Suwon , Gyeonggi-do , Korea.
authors.forename: Hyuk-Joon
authors.initials: HJ
authors.lastname: Jeon
authors.affiliation: b Department of East-West Medical Science , Graduate School of East-West Medical Science, Kyung Hee University , Yongin , Korea.
authors.forename: Dong-Hyung
authors.initials: DH
authors.lastname: Cho
authors.affiliation: c Biomedical Research Institute, MEDIPOST Co., Ltd. , Seongnam , Korea.
authors.forename: Hong Bae
authors.initials: HB
authors.lastname: Jeon
authors.affiliation: d Department of Neuroscience , Neurodegeneration Control Research Center, School of Medicine, Kyung Hee University , Seoul , Korea.
authors.forename: Sung Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: e Department of Health Sciences and Technology , Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Sungkyunkwan University , Seoul , Korea.
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Chang
authors.affiliation: f Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences , Seoul , Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kim
authors.affiliation: a Department of Genetic Engineering , College of Biotechnology and Bioengineering, Sungkyunkwan University , Suwon , Gyeonggi-do , Korea.
authors.forename: Jeong Su
authors.initials: JS
authors.lastname: Oh
mesh_terms: D000818:Animals; D001343:Autophagy; D000071183:Autophagy-Related Proteins / Q000235:genetics / Q000378:metabolism; D000071186:Beclin-1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D058546:Class III Phosphatidylinositol 3-Kinases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D048749:Cytokinesis / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008540:Meiosis; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D061251:Primary Cell Culture; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: C561107:Atg14 protein, mouse; D000071183:Autophagy-Related Proteins; D000071186:Beclin-1; C115010:Becn1 protein, mouse; D002352:Carrier Proteins; D016372:RNA, Antisense; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins; D033921:Vesicular Transport Proteins; D058546:Class III Phosphatidylinositol 3-Kinases
keywords: Beclin-1; autophagy; cytokinetic abscission; meiosis; oocyte