title: Reverse transcription-PCR assays for the differentiation of various US porcine epidemic diarrhea virus strains.
abstract: Concurrently, several porcine epidemic diarrhea virus (PEDV) variants are circulating in US swine farms, including the original US and the spike insertion-deletion (S-INDEL) strains. In this study, reverse transcription (RT)-PCR assays for the detection and differentiation of different US PEDV variants were developed based on the differences in the S1 domain of the spike (S) gene. This assay successfully differentiated three PEDV strains: PC22A (the original US virulent), Iowa106 (S-INDEL), and PC177 (S-197DEL) that was derived from cell culture adaptation and has a 197 amino acid-deletion in the S1 domain. The assays did not amplify the porcine deltacoronavirus OH-FD22 strain or transmissible gastroenteritis virus Miller strain. It is the first report on the development of RT-PCR assays allowing the detection and differentiation of all major types of US PEDV variants.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China; Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA. Electronic address: liuxinsheng@caas.cn.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA. Electronic address: wang.655@osu.edu.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D053485:Porcine epidemic diarrhea virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology; D014481:United States
chemical_list: D064370:Spike Glycoprotein, Coronavirus
keywords: Porcine epidemic diarrhea virus; Reverse transcription-PCR; Spike gene