title: Selenium and mercury levels in rat liver slices co-treated with diphenyl diselenide and methylmercury.
abstract: Organoseleno-compounds have been investigated for its beneficial effects against methylmercury toxicity. In this way, diphenyl diselenide (PhSe)2 was demonstrated to decrease Hg accumulation in mice, protect against MeHg-induced mitochondrial dysfunction, and protect against the overall toxicity of this metal. In the present study we aimed to investigate if co-treatment with (PhSe)2 and MeHg could decrease accumulation of Hg in liver slices of rats. Rat liver slices were co-treated with (PhSe)2 (0.5; 5 µM) and/or MeHg (25 µM) for 30 min at 37 °C and Se and Hg levels were measured by inductively coupled plasma mass spectrometry (ICP-MS) in the slices homogenate, P1 fraction, mitochondria and incubation medium. Co-treatment with (PhSe)2 and MeHg did not significantly alter Se levels in any of the samples when compared with compounds alone. In addition, co-treatment with (PhSe)2 and MeHg did not decrease Hg levels in any of the samples tested, although, co-incubation significantly increased Hg levels in homogenate. We suggest here that (PhSe)2 could exert its previously demonstrated protective effects not by reducing MeHg levels, but forming a complex with MeHg avoiding it to bind to critical molecules in cell.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil. crisbioq@yahoo.com.br.|Universidade Federal do Pampa, Campus Caçapava do Sul, Av. Pedro Anunciação, 111, Vila Batista, Caçapava do Sul, RS, 96570-000, Brazil. crisbioq@yahoo.com.br.|Dep. de Bioquímica e Biologia Molecular, CCNE, UFSM, Santa Maria, RS, 97105-900, Brazil. crisbioq@yahoo.com.br.
authors.forename: Cristiane Lenz
authors.initials: CL
authors.lastname: Dalla Corte
authors.affiliation: Departamento de Bioquímica e Biologia Molecular Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Angélica
authors.initials: A
authors.lastname: Ramos
authors.affiliation: Laboratório de Análises Químicas Industriais e Ambientais (LAQIA), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Clarissa Marques Moreira
authors.initials: CM
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Análises Químicas Industriais e Ambientais (LAQIA), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
authors.forename: Valderi Luiz
authors.initials: VL
authors.lastname: Dressler
authors.affiliation: Departamento de Bioquímica e Biologia Molecular Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil. jbtrocha@yahoo.com.br.|Dep. de Bioquímica e Biologia Molecular, CCNE, UFSM, Santa Maria, RS, 97105-900, Brazil. jbtrocha@yahoo.com.br.
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: da Rocha
mesh_terms: D000818:Animals; D001555:Benzene Derivatives / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry* / Q000187:drug effects*; D008297:Male; D013058:Mass Spectrometry; D008628:Mercury / Q000032:analysis*; D008767:Methylmercury Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000032:analysis*
chemical_list: D001555:Benzene Derivatives; D008767:Methylmercury Compounds; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D008628:Mercury; D012643:Selenium
keywords: ICP-MS; Liver slices; Methylmercury; Mitochondria; Selenium