title: Effect of Vanadium and Tea Polyphenols on Intestinal Morphology, Microflora and Short-Chain Fatty Acid Profile of Laying Hens.
abstract: Vanadium (V) is a trace element which can induce dysfunction of gastro-intestine and egg quality deterioration of laying hens. This study was conducted to determine the effect of tea polyphenols (TP) on intestinal morphology, microflora, and short-chain fatty acid (SCFA) profile of laying hens fed vanadium containing diets. A total of 120 Lohman laying hens (67-week-old) were randomly divided into 4 groups with 6 replicates and 5 birds each for a 35-day feeding trial. The dietary treatments were as follows: (1) control (CON), fed a basal diet; (2) vanadium treatment (V10), CON +10 mg V/kg; (3) TP treatment 1 (TP1): V10 + 600 mg TP/kg; (4) TP treatment 2 (TP2): V10 + 1000 mg TP/kg. Fed 10 mg V/kg diets to laying hens did not affect the cecum flora diversity index (H), degree of homogeneity (EH), and richness (S), but hens fed TP2 diet decreased the H, EH, and S (P < 0.05). The cecum butyrate acid concentration was lower in V10 treatment and higher in TP2 treatment (P < 0.05). Addition of 10 mg/kg V resulted in an increased (P < 0.01) duodenal cell apoptosis rate, and 1000 mg/kg TP supplementation overcame (P < 0.01) this reduction effect induced by vanadium. The results indicated that supplementation of 10 mg/kg vanadium increased duodenal cell apoptosis and reduced cecum butyrate acid content. Addition of 1000 mg/kg TP increased the SCFA production to affect cecum flora ecology and protected the duodenal cell from excess apoptosis caused by vanadium.
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Yuan
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China. wangjianping1983@hotmail.com.
authors.forename: J P
authors.initials: JP
authors.lastname: Wang
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: K Y
authors.initials: KY
authors.lastname: Zhang
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Ding
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: S P
authors.initials: SP
authors.lastname: Bai
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: Q F
authors.initials: QF
authors.lastname: Zeng
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xuan
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Su
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002432:Cecum / Q000378:metabolism / Q000382:microbiology; D002645:Chickens; D004386:Duodenum / Q000378:metabolism / Q000382:microbiology; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*; D014131:Trace Elements / Q000494:pharmacology*; D014639:Vanadium / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D059808:Polyphenols; D013662:Tea; D014131:Trace Elements; D014639:Vanadium
keywords: Apoptosis; Cecum microflora; Duodenum morphology; Laying hens; Tea polyphenols; Vanadium