title: Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis.
abstract: Aim. To explore the effects of curcumin on phosphate carrier (PiC) and its role in protection against doxorubicin induced myocyte toxicity. Methods. Using H9c2 cell line, the cardiotoxic effect of doxorubicin and its mitigation by curcumin were studied. H9c2 cells were cultured with doxorubicin and/or curcumin at various concentrations. Analysis for apoptosis of H9c2 was done using flow cytometry. Confocal laser scanning microscopy was used to record the fluorescence intensity ratios and to determine the mitochondrial permeability transition pore (MPTP) opening state. Oxidative stress was measured using glutathione level, superoxide dismutase activities, and malondialdehyde content. The effect of doxorubicin and curcumin on PiC gene expression was measured by real-time PCR. Results. Curcumin decreased mRNA of PiC and was partly protective against oxidative stress, loss of mitochondrial transmembrane potential, and apoptosis induced by doxorubicin. Interestingly, the effect was not clearly dose dependent and the concentration of 12 mg/L was more efficient than 15 and 10 mg/L. Conclusion. Curcumin downregulates PiC and partly protects against doxorubicin induced oxidative stress and myocyte apoptosis.
authors.affiliation: Department of Cardiology Division II, Tianjin Medical University Cardiovascular Institute, TEDA International Cardiovascular Hospital, Tianjin 300070, China; Department of Cardiology Division II, Jiamusi University, 1st Affiliated Hospital, Jiamusi, Heilongjiang 154003, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Junkun
authors.affiliation: Department of Cardiology Division II, Jiamusi University, 1st Affiliated Hospital, Jiamusi, Heilongjiang 154003, China.
authors.forename: Chu
authors.initials: C
authors.lastname: Erfu
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Hasahya
authors.initials: H
authors.lastname: Tony
authors.affiliation: Department of Cardiology Division II, Jiamusi University, 1st Affiliated Hospital, Jiamusi, Heilongjiang 154003, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xin
authors.affiliation: Department of Cardiology Division II, Jiamusi University, 1st Affiliated Hospital, Jiamusi, Heilongjiang 154003, China.
authors.forename: K C
authors.initials: KC
authors.lastname: Sudeep
authors.affiliation: Department of Cardiology Division II, Jiamusi University, 1st Affiliated Hospital, Jiamusi, Heilongjiang 154003, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Mingliang
authors.affiliation: Jiamusi University, Jiamusi, Heilongjiang 154003, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yanqin
authors.affiliation: Department of Cardiology Division II, Tianjin Medical University Cardiovascular Institute, TEDA International Cardiovascular Hospital, Tianjin 300070, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: XiangQian
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D003474:Curcumin / Q000008:administration & dosage*; D004317:Doxorubicin / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D028061:Phosphate Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D010710:Phosphates / Q000378:metabolism; D051381:Rats
chemical_list: D028061:Phosphate Transport Proteins; D010710:Phosphates; D004317:Doxorubicin; D003474:Curcumin