title: Chemical modification of coating of Pinus halepensis pollen by ozone exposure.
abstract: Pollen coating, located on the exine, includes an extractible lipid fraction. The modification of the pollen coating by air pollutants may have implications on the interactions of pollen with plant stigmas and human cells. Pinus halepensis pollen was exposed to ozone in vitro and the pollen coating was extracted with organic solvent and analyzed by GC-MS. Ozone has induced chemical changes in the coating as observed with an increase in dicarboxylic acids, short-chain fatty acids and aldehydes. 4-Hydroxybenzaldehyde was identified as the main reaction product and its formation was shown to occur both on native pollen and on defatted pollen. 4-Hydroxybenzaldehyde is very likely formed via the ozonolysis of acid coumaric-like monomers constitutive of the sporopollenin. Modification of pollen coating by air pollutants should be accounted for in further studies on effect of pollution on germination and on allergenicity.
authors.affiliation: Univ. Lille, CNRS, UMR 8522 - PC2A - Physicochimie des Processus de Combustion et de l'Atmosphère, F-59000, Lille, France; Laboratoire de Dispositifs Micro-ondes et Matériaux pour les Energies Renouvelables. DIMMER, Département d'Agronomie, Faculté des Sciences de la Nature et de la Vie, Equipe Biodiversité et Environnement, Université Ziane Achour, Djelfa, 17000, Algeria.
authors.forename: Oumsaad
authors.initials: O
authors.lastname: Naas
authors.affiliation: Univ. Lille, CNRS, UMR 8522 - PC2A - Physicochimie des Processus de Combustion et de l'Atmosphère, F-59000, Lille, France.
authors.forename: Maxence
authors.initials: M
authors.lastname: Mendez
authors.affiliation: Univ. Lille, CNRS, Univ. Littoral Côte d'Opale, UMR 8187 - LOG - Laboratoire d'Océanologie et de Géosciences, F-59000, Lille, France.
authors.forename: Melesio
authors.initials: M
authors.lastname: Quijada
authors.affiliation: Univ. Lille, CNRS, UMR 8522 - PC2A - Physicochimie des Processus de Combustion et de l'Atmosphère, F-59000, Lille, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Gosselin
authors.affiliation: Univ. Lille, CNRS, UMR 8522 - PC2A - Physicochimie des Processus de Combustion et de l'Atmosphère, F-59000, Lille, France; Lebanese University, Faculty of Public Health (FSP III), Water & Environment Science, Tripoli, Lebanon.
authors.forename: Jinane
authors.initials: J
authors.lastname: Farah
authors.affiliation: Etude et exploitation des propriétés technofonctionnelles des produits alimentaires, Laboratoire de Chimie Organique et Substances Naturelles, Département d'Agronomie, Faculté des Sciences de la Nature et de la Vie, Université Ziane Achour, Djelfa, 17000, Algeria.
authors.forename: Ali
authors.initials: A
authors.lastname: Choukri
authors.affiliation: Univ. Lille, CNRS, UMR 8522 - PC2A - Physicochimie des Processus de Combustion et de l'Atmosphère, F-59000, Lille, France. Electronic address: nicolas.visez@univ-lille1.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Visez
mesh_terms: D000393:Air Pollutants / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry*; D002338:Carotenoids / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000737:chemistry*; D010126:Ozone / Q000737:chemistry*; D028223:Pinus / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*
chemical_list: D000393:Air Pollutants; D001704:Biopolymers; D008055:Lipids; C009800:sporopollenin; D002338:Carotenoids; D010126:Ozone
keywords: Adjuvant effect; Allergy; Atmospheric chemistry; Pollenkitt; Sporopollenin