title: Increased Kappa/Lambda Hybrid Antibody in Serum Is a Novel Biomarker Related to Disease Activity and Inflammation in Rheumatoid Arthritis.
abstract: The κ/λ hybrid antibodies in normal human serum were reported recently, but their clinical relevance has not yet been explored. Rheumatoid arthritis (RA) is one of the major joint diseases, and the early diagnosis and treatment of RA remain a challenge. Here, we developed a double-sandwich enzyme-linked immunosorbent assay system to quantify relative serum κ/λ hybrid antibody levels in RA patients, osteoarthritis (OA) patients, and healthy controls (HC) in order to assess their potential use as a serological biomarker of early disease and clinical activity and to preliminarily investigate their immunomodulatory roles in RA. Surprisingly, we found that κ/λ hybrid antibody was markedly increased in both early and established RA. Serum κ/λ hybrid antibody levels were significantly correlated with clinical indexes and inflammatory markers in RA. Further analysis showed a positive correlation between κ/λ hybrid antibody levels and the 28-joint disease activity score (DAS28). In conclusion, serum κ/λ hybrid antibodies in RA were identified for the first time. High levels of κ/λ hybrid antibody may be a useful tool in distinguishing early RA from OA and HC. We suggest κ/λ hybrid antibody as a marker for disease activity. The increased κ/λ hybrid antibodies were associated with inflammatory conditions in RA.
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Yi
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Mingju
authors.initials: M
authors.lastname: Hao
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Lu
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China.
authors.forename: Guigao
authors.initials: G
authors.lastname: Lin
authors.affiliation: China-Japan Friendship Hospital, Beijing 100730, China.
authors.forename: Lida
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Rheumatology, Beijing Hospital, Beijing 100730, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Gaowei
authors.initials: G
authors.lastname: Fan
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Guojing
authors.initials: G
authors.lastname: Wang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China.
authors.forename: Kuo
authors.initials: K
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China.
authors.forename: Yanxi
authors.initials: Y
authors.lastname: Han
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China.
authors.forename: Lunan
authors.initials: L
authors.lastname: Wang
authors.affiliation: National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007145:Immunoglobulin kappa-Chains / Q000097:blood*; D007146:Immunoglobulin lambda-Chains / Q000097:blood*; D007136:Immunoglobulins / Q000097:blood*; D007249:Inflammation / Q000097:blood*; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins