title: Antimicrobial Hyaluronic Acid-Cefoxitin Sodium Thin Films Produced by Electrospraying.
abstract: The healing properties of hyaluronic acid (HA) in the recovery of wounds are well known. Cefoxitin (Cef), a cephalosporin antibiotic, is generally used to prevent and treat postoperative infections. In this study, we describe the incorporation of Cef in HA thin films (Cef-HAF) by using electrospraying. Scanning electron microscopy images showed that HA-containing thin films (HAF) were composed of numerous nanoparticles (255 ± 177 nm in diameter) with irregular surfaces, connected to each other with nanofibers of 50 ± 11 nm in diameter. Cef-HAF contained fewer, but larger, particles (551 ± 293 nm) with smooth surfaces and were interconnected with nanofibers of 61 ± 13 nm in diameter. Differences in surface morphology between HAF and Cef-HAF were confirmed by atomic force microscopy. Fourier transform infrared and X-ray diffraction analyses revealed that Cef was not modified when incorporated into Cef-HAF and remained active against Klebsiella pneumoniae Xen 39, Staphylococcus aureus Xen 36 and Listeria monocytogenes EDGe. Nanofiber scaffolds of HA-containing Cef may be used in dressings to control postoperative infections.
authors.affiliation: Department of Microbiology, University of Stellenbosch, Matieland, Stellenbosch, 7602, South Africa.
authors.forename: Jayesh J
authors.initials: JJ
authors.lastname: Ahire
authors.affiliation: Department of Microbiology, University of Stellenbosch, Matieland, Stellenbosch, 7602, South Africa. lmtd@sun.ac.za.
authors.forename: Leon M T
authors.initials: LM
authors.lastname: Dicks
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002440:Cefoxitin / Q000737:chemistry / Q000494:pharmacology*; D055664:Electrochemical Techniques; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D002440:Cefoxitin; D006820:Hyaluronic Acid