title: [Polymorphism of Platelet Specific Antigens (HPA-1-5, 15) in Han Donors in the North Area of Henan Province].
abstract: OBJECTIVE
  To study the gene polymorphism distribution characteristics of human platelet HPA-1-5 and 15 blood group antigens and construct a certain scale of platelet HPA database in the north area of Henan Province so as to provide platelet apheresis for clinical departments.
  
  
  METHODS
  Using polymerase chain reaction with sequence-specific primers (PCR-SSP), the genotyping of HPA-1-5 and 15 system was carried out; the periperal blood of 500 healthy Han donors in north area of Henan Province was collected randomly, the gene and genotype frequencies were detected by direct counting method, and the population distribution frequncy of HPA genes was analyzed by Hardy-Weinberg balance test, and compared with other regions and ethnics by using χ(2) test.
  
  
  RESULTS
  There was statistically significant (P < 0.05) of increase HPA-3b and HPA-5a in North area of Henan Province, compared with Chinese Han population; the HPA-3b and 5a increase and HPA-2a decrease were statistically significant (P < 0.05), compared with Ethnic minority of China. There was partly increase of HPA-1a, 2a, 3a and 5a, compared with different regions and ethnic in abroad. HPA allele genes of 500 Han donors in the North area of Henan Province were as follows: 0.985 and 0.015 for 1a and 1b; 0.924 and 0.076 for 2a and 2b; 0.469 and 0.531 for 3a and 3b; 1.000 and 1.000 for 4a and 5a; 0.532 and 0.468 for 15a and 15b, respectively. HPA allele gene frequencies were 1aa0.970, 1ab0.030; 2aa0.848, 2ab0.152; 3aa0.222, 3ab0.494, 3bb0.284; 4aa1.000; 5aa1.000; 15aa0.282, 15ab0.500, 15bb0.218. Compared with other regions and ethnic, HPA gene frequencies partly had statistical significance.
  
  
  CONCLUSIONS
  Distribution of HPA allele frequencies in the North area of Henan province is in accordence with the Hardy-Weinberg law. There are race and regional differences in HPA allele gene frequencies, compared with other regions and countries. And the HPA systems HPA-3 and 15 display the genetic polymorphisms, which provides a theoretical basis for the relevant research of the same type platelet infusion and alloimmune thrombocytopenia.
authors.affiliation: Henan Province Collaborative Innovation Center of Molecular Diagnosis and Laboratorial Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Xue-Lan
authors.initials: XL
authors.lastname: Sun
authors.affiliation: Henan Province Collaborative Innovation Center of Molecular Diagnosis and Laboratorial Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Zhi-Mei
authors.initials: ZM
authors.lastname: Yang
authors.affiliation: Henan Province Collaborative Innovation Center of Molecular Diagnosis and Laboratorial Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: General Hospital of Puyang Oil Field, Puyang 457001, Henan Province, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Yuan
authors.affiliation: Henan Blood Center, Zhengzhou 45000, Henan Province, China.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Li
authors.affiliation: Xinxiang Blood Center, Xinxiang 453000, Henan Province, China.
authors.forename: Gui-Zhi
authors.initials: GZ
authors.lastname: Pang
authors.affiliation: Henan Province Collaborative Innovation Center of Molecular Diagnosis and Laboratorial Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China. E-mail: 051085@xxmu.edu.cn.
authors.forename: Chen-Guang
authors.initials: CG
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D015703:Antigens, CD / Q000235:genetics*; D016824:Antigens, Human Platelet / Q000235:genetics*; D001792:Blood Platelets; D002681:China; D017931:DNA Primers; D005006:Ethnicity; D058851:GPI-Linked Proteins / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D010983:Plateletpheresis; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D015703:Antigens, CD; D016824:Antigens, Human Platelet; C120523:CD109 protein, human; D017931:DNA Primers; D058851:GPI-Linked Proteins; D009363:Neoplasm Proteins