title: A novel method of extraction of bamboo seed oil (Bambusa bambos Druce) and its promising effect on metabolic symptoms of experimentally induced polycystic ovarian disease.
abstract: OBJECTIVE
  To evaluate the potential effect of bamboo seed oil in decreasing the major metabolic symptoms associated with letrozole-induced polycystic ovarian disease using female rat model.
  
  
  METHODS
  A new method of microwave-assisted extraction was developed. Female rats were grouped into four with six animals each. All rats were daily administered with letrozole (1 mg/kg b.wt.) for 21 days except control, and during this period, changes in estrous cycle were observed. After letrozole treatment, Group 2 was considered negative control, Groups 3 and 4 were treated orally with bamboo oil, 0.5 ml/kg b.wt. and 1 ml/kg b.wt., respectively, for 3 weeks (five consecutive estrus cycles). Various parameters such as estrus cycle, blood sugar level, lipid profile, and weights of reproductive system were determined. The characteristics of cystic ovaries were evaluated by histopathological studies.
  
  
  RESULTS
  The isolated bamboo oil restored estrus cyclicity showed hypoglycemic and hypolipidemic effects. 1 ml/kg b.wt. of bamboo oil showed a marked glucose reduction from 254.04 ± 2.08 to 92.6 ± 1.63, and levels of total cholesterol, very low-density lipoprotein, triglyceride were reduced from 186.45 ± 2.28, 30.07 ± 2.36, 100.36 ± 2.35 to 152.14 ± 2.63, 25.94 ± 1.66, 93.32 ± 1.09, respectively. Histopathological results showed the presence of ovulation and recovery from cystic ovaries.
  
  
  CONCLUSIONS
  A novel and promising drug was isolated in the treatment and maintenance of various metabolic symptoms associated with polycystic ovary disease.
authors.affiliation: Department of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
authors.forename: V
authors.initials: V
authors.lastname: Soumya
authors.affiliation: Department of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
authors.forename: Y Indira
authors.initials: YI
authors.lastname: Muzib
authors.affiliation: Department of Pharmaceutical Chemistry, Sir C R Reddy College of Pharmaceutical Sciences, Eluru, Andhra Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Venkatesh
mesh_terms: D000818:Animals; D031723:Bambusa / Q000196:embryology*; D005260:Female; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D010938:Plant Oils / Q000302:isolation & purification* / Q000627:therapeutic use*; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*
chemical_list: D007004:Hypoglycemic Agents; D010938:Plant Oils
keywords: Bamboo seed oil; Bambusa bambos Druce; cystic ovary; estrus cycle; lipid profile; polycystic ovary disease; polycystic ovary syndrome