title: RCA-Based Biosensor for Electrical and Colorimetric Detection of Pathogen DNA.
abstract: For the diagnosis and prevention of diseases, a range of strategies for the detection of pathogens have been developed. In this study, we synthesized the rolling circle amplification (RCA)-based biosensor that enables detection of pathogen DNA in two analytical modes. Only in the presence of the target DNA, the template DNA can be continuously polymerized by simply carrying out RCA, which gives rise to a change of surface structure of Au electrodes and the gap between the electrodes. Electrical signal was generated after introducing hydrogen tetrachloroaurate (HAuCl4) to the DNA-coated biosensor for the improvement of the conductivity of DNA, which indicates that the presence of the pathogen DNA can be detected in an electrical approach. Furthermore, the existence of the target DNA was readily detected by the naked eyes through change in colors of the electrodes from bright yellow to orange-red after RCA reaction. The RCA-based biosensor offers a new platform for monitoring of pathogenic DNA with two different detection modes in one system.
authors.affiliation: Department of Chemical Engineering, University of Seoul, Seoul, 130-743, South Korea.
authors.forename: Jaepil
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Department of Chemical Engineering, University of Seoul, Seoul, 130-743, South Korea.
authors.forename: Hyejin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering, University of Seoul, Seoul, 130-743, South Korea.
authors.forename: Dong Jun
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering, University of Seoul, Seoul, 130-743, South Korea.
authors.forename: Byung Jun
authors.initials: BJ
authors.lastname: Jung
authors.affiliation: Department of Chemical Engineering, University of Seoul, Seoul, 130-743, South Korea. jblee@uos.ac.kr.
authors.forename: Jong Bum
authors.initials: JB
authors.lastname: Lee
keywords: Biosensor; DNA detection; Electrical detection; Multi-primer; Rolling circle amplification