title: Prolongation of survival of dogs with oral malignant melanoma treated by en bloc surgical resection and adjuvant CSPG4-antigen electrovaccination.
abstract: Reported post-surgery 1-year survival rate for oral canine malignant melanoma (cMM) is around 30%; novel treatments are needed as the role of adjuvant chemotherapy is unclear. This prospective study regards adjuvant electrovaccination with human chondroitin sulfate proteoglycan-4 (hCSPG4)-encoded plasmid in 23 dogs with resected II/III-staged CSPG4-positive oral cMM compared with 19 dogs with resected only II/III-staged CSPG4-positive oral cMM. Vaccination resulted in 6-, 12-, 18- and 24-month survival rate of 95.6, 73.9, 47.8 and 30.4%, respectively [median survival time (MST) 684 days, range 78-1694, 8 of 23 dogs alive] and 6-, 12-, 18- and 24-month disease-free interval (DFI) rate of 82.6, 47.8, 26.1 and 17.4%, respectively (DFI 477 days, range 50-1694). Non-vaccinated dogs showed 6-, 12-, 18- and 24-month survival rate of 63.2, 26.3, 15.8 and 5.3%, respectively (MST 200 days, range 75-1507, 1 of 19 dogs alive) and 6-, 12-, 18- and 24-month DFI rate of 52.6, 26.3, 10.5 and 5.3%, respectively (DFI 180 days, range 38-1250). Overall survival and DFI of vaccinated dogs was longer in those <20 kg. In vaccinated and non-vaccinated dogs local recurrence rate was 34.8 and 42%, respectively while lung metastatic rate was 39 and 79%, respectively.
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: L A
authors.initials: LA
authors.lastname: Piras
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Riccardo
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Iussich
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Maniscalco
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gattino
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Martano
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Morello
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Lorda Mayayo
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Rolih
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Garavaglia
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: R
authors.initials: R
authors.lastname: De Maria
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Lardone
authors.affiliation: Clinica Veterinaria Roma Sud, Roma, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Collivignarelli
authors.affiliation: Clinica Veterinaria Roma Sud, Roma, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Mignacca
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Giacobino
authors.affiliation: Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: S
authors.initials: S
authors.lastname: Ferrone
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Cavallo
authors.affiliation: Department of Veterinary Sciences, University of Torino, Torino, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Buracco
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D019496:Cancer Vaccines / Q000627:therapeutic use; D011508:Chondroitin Sulfate Proteoglycans / Q000276:immunology*; D003131:Combined Modality Therapy; D004283:Dog Diseases / Q000401:mortality / Q000628:therapy*; D004285:Dogs; D005260:Female; D008297:Male; D008545:Melanoma / Q000401:mortality / Q000628:therapy / Q000662:veterinary*; D009062:Mouth Neoplasms / Q000401:mortality / Q000628:therapy / Q000662:veterinary*
chemical_list: D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D011508:Chondroitin Sulfate Proteoglycans
keywords: CSPG4; DNA electroporation; adjuvant immunotherapy; canine oral malignant melanoma; comparative oncology