title: Prevention effect of rare ginsenosides against stress-hormone induced MTOC amplification.
abstract: Stress has been suggested as one of important cause of human cancer without molecular biological evidence. Thus, we test the effect of stress-related hormones on cell viability and mitotic fidelity. Similarly to estrogen, stress hormone cortisol and its relative cortisone increase microtubule organizing center (MTOC) number through elevated expression of γ-tubulin and provide the Taxol resistance to human cancer cell lines. However, these effects are achieved by glucocorticoid hormone receptor (GR) but not by estrogen receptor (ER). Since ginsenosides possess steroid-like structure, we hypothesized that it would block the stress or estrogen-induced MTOC amplification and Taxol resistance. Among tested chemicals, rare ginsenoside, CSH1 (Rg6) shows obvious effect on inhibition of MTOC amplification, γ-tubulin induction and Taxol resistance. Comparing to Fulvestant (FST), ER-α specific inhibitor, this chemical can block the cortisol/cortisone-induced MTOC deregulation as well as ER-α signaling. Our results suggest that stress hormone induced tumorigenesis would be achieved by MTOC amplification, and CSH1 would be useful for prevention of stress-hormone or steroid hormone-induced chromosomal instability.
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Chun
authors.affiliation: College of Pharmacy, Chungnam National University, Daejoen, Korea.
authors.forename: Jung Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungnam National University, Daejoen, Korea.
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungnam National University, Daejoen, Korea.
authors.forename: Kyu Jin
authors.initials: KJ
authors.lastname: Cheong
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Youn-Sang
authors.initials: YS
authors.lastname: Jung
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Tae-Gyun
authors.initials: TG
authors.lastname: Woo
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Yoon
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Oh
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: So-Mi
authors.initials: SM
authors.lastname: Kang
authors.affiliation: Department of Statistics, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Chunghui
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Statistics, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Hokeun
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Microbiology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Jihwan
authors.initials: J
authors.lastname: Hwang
authors.affiliation: College of Pharmacy, Chungnam National University, Daejoen, Korea.
authors.forename: Gyu-Yong
authors.initials: GY
authors.lastname: Song
authors.affiliation: Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea.
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Park
mesh_terms: D045744:Cell Line, Tumor; D003348:Cortisone / Q000494:pharmacology*; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D022101:Microtubule-Organizing Center / Q000187:drug effects*; D017239:Paclitaxel / Q000494:pharmacology; D013315:Stress, Psychological / Q000150:complications* / Q000378:metabolism / Q000473:pathology
chemical_list: D036145:Ginsenosides; D017239:Paclitaxel; D003348:Cortisone; D006854:Hydrocortisone
keywords: MTOC; cancer prevention; stress hormone