title: A versatile fluorescent biosensor based on target-responsive graphene oxide hydrogel for antibiotic detection.
abstract: A fluorescent sensing platform based on graphene oxide (GO) hydrogel was developed through a fast and facile gelation, immersion and fluorescence determination process, in which the adenosine and aptamer worked as the co-crosslinkers to connect the GO sheets and then form the three-dimensional (3D) macrostructures. The as-prepared hydrogel showed high mechanical strength and thermal stability. The optimal hydrogel had a linear response for oxytetracycline (OTC) of 25-1000μg/L and a limit of quantitation (LOQ) of 25μg/L. Moreover, together with the high affinity of the aptamer for its target, this assay exhibited excellent sensitivity and selectivity. According to its design principle, the as-designed hydrogel was also tested to possess the generic detection function for other molecules by simply replacing its recognition element, which is expected to lay a foundation to realize the assembly of functionalized hierarchical graphene-based materials for practical applications in analytical field.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Tan
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China. Electronic address: zhaohuim@dlut.edu.cn.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Gan
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
authors.forename: Xie
authors.initials: X
authors.lastname: Quan
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D060766:Drinking Water / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry; D010118:Oxytetracycline / Q000032:analysis*; D045483:Rivers / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013412:Sulfadimethoxine / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D060766:Drinking Water; D005456:Fluorescent Dyes; D010087:Oxides; D014874:Water Pollutants, Chemical; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D013412:Sulfadimethoxine; D006108:Graphite; D010118:Oxytetracycline
keywords: Antibiotic; Aptamer; Fluorescent detection; GO hydrogel; Oxytetracycline; Sulfadimethoxine