title: A quick response fluorescent probe based on coumarin and quinone for glutathione and its application in living cells.
abstract: We have designed and synthesized a simple but effective fluorescent probe for sensing glutathione (GSH) by PET process based on coumarin and quinone, which worked as fluorophore and reaction site, respectively. The probe could discriminate GSH from cysteine and homocysteine within 1 min in PBS-buffered solution. The sensing mechanism was confirmed by density functional theory (DFT), viscosity test, fluorescence spectrum analysis and HRMS, respectively. The probe has a low limit of detection (0.1 μM) and finally been used in cell imaging successfully.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Dai
authors.affiliation: Taishan College, Shandong University, Jinan 250100, PR China.
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Du
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D016227:Benzoquinones / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D016227:Benzoquinones; D003374:Coumarins; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; C004532:quinone; C030123:coumarin; D005978:Glutathione
keywords: Coumarin; Fluorescent probe; Glutathione; Living cells; Quinone