title: [Effects of Bone Marrow Mesenchymal Stem Cells on the Biological Characteristics of Migrating and Homing in Multiple Myeloma Cells].
abstract: OBJECTIVE
  To construct a co-culture system for bone marrow mesenchymal stem cells (BMMSC) and multiple myeloma (MM) cells, and to investigate the effects of co-cultured BMMSC on the migrating and homing of multiple myeloma cells.
  
  
  METHODS
  The BMMSC from the transgenic mice with green fluorescent protein (GFP) fetal bone were cultured by adherent screening. A co-culture system of BMMSC and MM cell line XG-7 cells was constracted, the proliferation and apoptosis of cells were determined by trypan blue exclusion and Annexin V/PI, respectively, MDC staining was employed to detect the autophagy. The moving direction distribution of molecule in BMMSC and XG-7 cells labeled with PE-CD138 in co-culture process were observed dinamically by confocal microscopy.
  
  
  RESULTS
  After co-culture with GFP-BMMSC, the resistance of XG-7 cells to apoptosis and autophagy were enhanced; at the same time, their proliferation increased. Apoptosis rates of XG-7 cells directly and indirectly co-cultured with BMMSC were (6.23 ± 0.12)% and (6.97 ± 0.03)% respectively, which were lower than that of XG-7 cells cultured alone (17.90 ± 1.46)% (P < 0. 01). There was low level of autophagy in XG-7 cells co-cultured with BMMSC. XG-7 cells are highly polarized and contained a specialized membrane domain with specific protein and lipid components to contact with BMMSC under confocal microscope. After methyl-β-cyclodextrin treatment, the molecules were normally enriched in the specialized domain.
  
  
  CONCLUSIONS
  BMMSC can protect XG-7 cells from apoptosis and autophagy, and obviously promote the proliferation of XG-7 cells, and can influence the migrating and homing of multiple myeloma cells.
authors.affiliation: Department of Hematology, Ningbo First Hospital, Ningbo 315010, Zhejiang Province, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Second Affiliated Hospital of Soochou University, Suzhou 215004, Jiangsu Province, China.
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Fu
authors.affiliation: Department of Hematology, Second Affiliated Hospital of Soochou University, Suzhou 215004, Jiangsu Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Ningbo First Hospital, Ningbo 315010, Zhejiang Province, China.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Zhu
authors.affiliation: Department of Hematology, Ningbo First Hospital, Ningbo 315010, Zhejiang Province, China. E-mail: ouyangguifang@medmail.com.cn.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D001854:Bone Marrow Cells / Q000166:cytology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D018920:Coculture Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D009101:Multiple Myeloma / Q000473:pathology*