title: AttenuatedToxoplasma gondiitherapy of disseminated pancreatic cancer generates long-lasting immunity to pancreatic cancer.
abstract: We have recently reported that treatment of disseminated pancreatic cancer with an attenuatedToxoplasma gondiiuracil auxotroph vaccine promoted antitumor CD8+T cell responses and long-term survival. Here, we optimized the treatment strategy for disseminated pancreatic cancer and show that attenuatedToxoplasma gondiitherapy stimulated effective long-term immunity to pancreatic cancer through mechanisms involving CD4+T cells and pancreatic tumor-specific IgG. Our results suggest that cell-mediated immunity in conjunction with humoral antibody immunity may offer greater resistance to recurrence of highly aggressive tumors. Cancer immunotherapeutic strategies using attenuatedToxoplasma gondiivaccines merit further investigation as a novel strategy to reawaken immunity to primary pancreatic carcinoma and to generate long-lasting immunity to pancreatic cancer recurrences.
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth , Lebanon, NH, USA.
authors.forename: Kiah L
authors.initials: KL
authors.lastname: Sanders
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth , Lebanon, NH, USA.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Fox
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth , Lebanon, NH, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Bzik
keywords: Humoral immunity; T cell protection; Toxoplasma gondii; immunotherapy; pancreatic cancer; tumor-specific antibodies