title: Dengue Patients with Early Hemorrhagic Manifestations Lose Coordinate Expression of the Anti-Inflammatory Cytokine IL-10 with the Inflammatory Cytokines IL-6 and IL-8.
abstract: Dengue is responsible for a wide range of clinical manifestations, ranging from asymptomatic infections to severe cases. The alteration of cytokine levels correlated with clinical characteristics can help determine prognostic markers of the disease and the identification of targets for immunotherapy. We measured the viral load, serotype, and cytokine levels of 212 serum samples from patients with acute dengue infection during days 1-4 after the onset of symptoms. The patients were classified as either with hemorrhagic manifestations (HM) or with no hemorrhagic manifestations (NHM). The cytokines interleukin-6 (IL-6), IL-8, and IL-10 were increased (P < 0.05) in the dengue virus+ group, compared with the control group. A higher viral load (P < 0.05) and IL-6 was detected in the HM group compared with the NHM group. Interestingly, the NHM group demonstrated a significant positive correlation between inflammatory (IL-6 and 8) and anti-inflammatory (IL-10) cytokines, whereas the HM group did not. These findings suggest that a disturbance in the balance of inflammatory cytokines IL-6 and IL-8 with the anti-inflammatory cytokine, IL-10, combined with the high levels of IL-6 and viral load, characterize possible mechanisms related to the formation of HM.
authors.affiliation: Divisão de Epidemiologia e Controle de Doenças/Instituto Octávio Magalhães, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil. Programa de Pós-graduação em Medicina e Biomedicina, Instituto de Ensino e Pesquisa, Hospital Santa Casa, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Felipe Campos de Melo
authors.initials: FC
authors.lastname: Iani
authors.affiliation: Divisão de Plataformas Tecnológicas/Diretoria de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Caldas
authors.affiliation: Divisão de Epidemiologia e Controle de Doenças/Instituto Octávio Magalhães, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Myrian Morato
authors.initials: MM
authors.lastname: Duarte
authors.affiliation: Divisão de Epidemiologia e Controle de Doenças/Instituto Octávio Magalhães, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ana Luisa Furtado
authors.initials: AL
authors.lastname: Cury
authors.affiliation: Divisão de Plataformas Tecnológicas/Diretoria de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Alzira Batista
authors.initials: AB
authors.lastname: Cecílio
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Pedro Augusto Carvalho
authors.initials: PA
authors.lastname: Costa
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Lis R
authors.initials: LR
authors.lastname: Antonelli
authors.affiliation: Programa de Pós-graduação em Medicina e Biomedicina, Instituto de Ensino e Pesquisa, Hospital Santa Casa, Belo Horizonte, Minas Gerais, Brazil. Institutos Nacionais de Ciências e Tecnologia-Doenças Tropicais, Belo Horizonte, Minas Gerais, Brazil. Núcleo de Ensino e Pesquisa, Instituto Mario Penna, Belo Horizonte, Minas Gerais, Brazil. kjgollob@gmail.com.
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Gollob
mesh_terms: D000293:Adolescent; D000328:Adult; D003715:Dengue / Q000097:blood* / Q000175:diagnosis / Q000276:immunology*; D003716:Dengue Virus / Q000302:isolation & purification; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000302:isolation & purification; D019562:Viral Load; D055815:Young Adult
chemical_list: C508606:CXCL8 protein, human; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D012367:RNA, Viral; D016753:Interleukin-10