title: The impact of multiple sclerosis relapse treatment on migration of effector T cells--Preliminary study.
abstract: Migration of inflammatory cells from the blood to the central nervous system (CNS) is crucial for development of multiple sclerosis (MS). Inhibition of this process would allow to control disease activity. The first step confirming this approach would be the analysis of the impact of effective MS relapse therapy on migration of effector T cells. The aim of the study was to analyze the influence of methylprednisolone (MP) on the migratory activity of effector CD4+ T cells from MS patients. Moreover, to study the potential mechanism of this process we studied expression of chemokine receptors on migrating cells.
  
  
  METHODS
  Peripheral blood samples were obtained from relapsing-remitting MS (RR-MS) patients during relapse (n=23) and from control group (n=23). After isolation CD4+ T cells were incubated with various concentrations of MP. Then they were stimulated in chemotaxis assay with chemokines CCL3 or CXCL10 or were used to CCR1 and CXCR3 expression analysis.
  
  
  RESULTS
  CXCL10- and CCL3-stimulated migration of CD4+ T cells was significantly increased in MS. MP was able to reduce in vitro migration of effector T cells induced by CXCL10, but not by CCL3. Inhibition by MP was dose-dependent. Expression of analyzed chemokine receptors was unaltered after MP incubation.
  
  
  CONCLUSIONS
  MP reduced CD4+ T cells migration induced by CXCL10 without affecting CXCR3 expression. These observations demonstrate one of the potential mechanisms of MP action in MS, distinct from inducing cell apoptosis, and suggests the new targets for development of more effective MS treatments.
authors.affiliation: Department of Propedeutics of Neurology, Medical University of Lodz, Lodz, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Jatczak-Pawlik
authors.affiliation: Department of Propedeutics of Neurology, Medical University of Lodz, Lodz, Poland; Department of Neurology and Stroke, Medical University of Lodz, Lodz, Poland. Electronic address: dominika.ksiazek@umed.lodz.pl.
authors.forename: Dominika
authors.initials: D
authors.lastname: Książek-Winiarek
authors.affiliation: Department of Propedeutics of Neurology, Medical University of Lodz, Lodz, Poland; Department of Neurology and Stroke, Medical University of Lodz, Lodz, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Wojkowska
authors.affiliation: Department of Propedeutics of Neurology, Medical University of Lodz, Lodz, Poland; Department of Neurology and Stroke, Medical University of Lodz, Lodz, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Jóźwiak
authors.affiliation: Department of Neurology and Stroke, Medical University of Lodz, Lodz, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Jastrzębski
authors.affiliation: Department of Laboratory Diagnostics, Medical University of Lodz, Lodz, Poland.
authors.forename: Mirosława
authors.initials: M
authors.lastname: Pietruczuk
authors.affiliation: Department of Propedeutics of Neurology, Medical University of Lodz, Lodz, Poland; Department of Neurology and Stroke, Medical University of Lodz, Lodz, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Głąbiński
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D018925:Chemokines / Q000276:immunology*; D002634:Chemotaxis, Leukocyte; D004305:Dose-Response Relationship, Drug; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008775:Methylprednisolone / Q000494:pharmacology*; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy* / Q000276:immunology; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000187:drug effects*
chemical_list: D018925:Chemokines; D005938:Glucocorticoids; D019707:Receptors, Chemokine; D008775:Methylprednisolone
keywords: Chemokine receptors; Chemokines; Methylprednisolone; Migration; Multiple sclerosis