title: High-titer lactic acid production by Lactobacillus pentosus FL0421 from corn stover using fed-batch simultaneous saccharification and fermentation.
abstract: Because the cost of refined sugar substrate and limit of worldwide food availability, lignocellulosic materials are attractive for use in lactic acid (LA) production. In this study, we found Lactobacillus pentosus strain FL0421 produced LA with high yields (0.52-0.82g/g stover) from five NaOH-pretreated and washed agro stovers through simultaneous saccharification and fermentation (SSF). We developed a fed-batch SSF process at 37°C and pH 6.0 using the cellulase of 30FPU/g stover and 10g/L yeast extract in a 5-L bioreactor to produce LA from 14% (w/w) NaOH-pretreated and washed corn stover under non-sterile condition. The LA-titer, yield and productivity reached 92.30g/L, 0.66g/g stover and 1.92g/L/h, respectively; and acetic acid titer and yield reached 34.27g/L and 0.24g/g stover. This study presented a feasible process for LA production from agro stovers and provided a candidate strain for genetic engineering for high-titer and -yield lignocellulosic LA production.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Yanxu
authors.initials: Y
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Zhenting
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Mei
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430068, Hubei, PR China.
authors.forename: Shumiao
authors.initials: S
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430068, Hubei, PR China.
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Hubei Collaborative Innovation Center for Industrial Fermentation, Wuhan 430068, Hubei, PR China; Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, Sichuan, PR China. Electronic address: nanp@mail.hzau.edu.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Peng
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D002480:Cellulase / Q000737:chemistry; D005285:Fermentation; D006031:Glycosylation; D019344:Lactic Acid / Q000096:biosynthesis*; D000070018:Lactiplantibacillus pentosus / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry
chemical_list: D019344:Lactic Acid; D002480:Cellulase
keywords: Corn stover; Lactic acid fermentation; Lactobacillus pentosus; Simultaneous saccharification and fermentation