title: Promotion of angiogenesis and proliferation cytokines patterns in peritoneal fluid from women with endometriosis.
abstract: Studies have long sought specific cytokines that could characterize endometriosis. Either due to variations between study designs regarding the assessment criteria for the cytokine or to low power resulting from small sample size, no factor proved to be sufficiently specific to endometriosis. In other clinical fields, a combination of several markers proved to be more powerful than a single-molecule approach. As well, in the context of endometriosis, simultaneous assessment of several cytokines present in the peritoneal fluid might help in unveiling patho-physiological processes, thus contributing to a better understanding of the condition. Therefore, the objective of this study was to investigate peritoneal fluid cytokines-derived of endometriotic women. For this retrospective case-control study, peritoneal fluid samples were obtained at laparoscopy and assessed by multiplex. Our data showed distinct patterns of peritoneal fluid cytokine concentrations in endometriotic women most notably a marked increase in EGF, FGF-2, IL-1α, MIP-1β, TGFα, PDGF-AA, PDGF-BB, MCP-3, sCD40L, Gro Pan, IL-17α, MDC and Rantes. The overall effect of fertility status revealed a significant difference for only one cytokine, namely MDC. Furthermore, FLT-3L and IP-10 levels were decreased in endometriosis patients, the former in both menstrual cycle phases and the latter in the secretory phase. A significant inverse Pearson correlation (p<0.05) was noted between pro-angiogenic cytokines EGF and FGF and the anti-angiogenic cytokine IP-10 in endometriosis patients at stages III-IV and in the secretory phase. These changes may exacerbate the local peritoneal angiogenic and proliferative reaction observed in women with endometriosis, and contributes to its pathophysiology.
authors.affiliation: Laboratory of Immunology, Research Center, Centre Hospitalier Universitaire de Québec, Faculty of Medicine, Laval University, Québec City G1V 4G2, Canada.
authors.forename: Halima
authors.initials: H
authors.lastname: Rakhila
authors.affiliation: Laboratory of Immunology, Research Center, Centre Hospitalier Universitaire de Québec, Faculty of Medicine, Laval University, Québec City G1V 4G2, Canada.
authors.forename: Mahera
authors.initials: M
authors.lastname: Al-Akoum
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Laval University, Québec City G1V 4G2, Canada.
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Bergeron
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Laval University, Québec City G1V 4G2, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Leboeuf
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Laval University, Québec City G1V 4G2, Canada.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Lemyre
authors.forename: Ali
authors.initials: A
authors.lastname: Akoum
authors.affiliation: Laboratory of Immunology, Research Center, Centre Hospitalier Universitaire de Québec, Faculty of Medicine, Laval University, Québec City G1V 4G2, Canada. Electronic address: marc.pouliot@crchul.ulaval.ca.
authors.forename: Marc
authors.initials: M
authors.lastname: Pouliot
mesh_terms: D001202:Ascitic Fluid / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D054422:Chemokine CCL22 / Q000378:metabolism*; D054357:Chemokine CXCL10 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004715:Endometriosis / Q000276:immunology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D009389:Neovascularization, Pathologic; D012189:Retrospective Studies; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; C515482:CCL22 protein, human; C114753:CXCL10 protein, human; D054422:Chemokine CCL22; D054357:Chemokine CXCL10; D016207:Cytokines; D008565:Membrane Proteins; C084881:flt3 ligand protein; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor
keywords: Angiogenesis; Cytokines; Endometriosis; Peritoneal fluid; Proliferation