title: Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological conditions.
abstract: We present here a novel and efficient method for 5mC detection using a DNA strand exchange reaction (SER) strategy. This enzyme-free method needs no pre-treatment of target DNAs and can be adapted to most of the target duplexes under physiological conditions. The high sequence selectivity of this method can distinguish 5mC from normal cytosine in an accurate manner.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.forename: Jinjun
authors.initials: J
authors.lastname: Wu
authors.forename: Wenting
authors.initials: W
authors.lastname: Liu
authors.forename: Tingting
authors.initials: T
authors.lastname: Hong
authors.forename: Tianlu
authors.initials: T
authors.lastname: Wang
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Zhang
authors.forename: Boshi
authors.initials: B
authors.lastname: Fu
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer
chemical_list: D044503:5-Methylcytosine; D004247:DNA