title: Serological under expression of microRNA-21, microRNA-34a and microRNA-126 in colorectal cancer.
abstract: OBJECTIVE
  This paper describes the ability of miRNA value predict oncological outcomes in CRC patients and correlates to clinical and pathologic variables.
  
  
  METHODS
  We prospectively analyzed the serological expression of microRNA-21, microRNA-34a, and microRNA-126 in 37 stage II - IV CRC patients and correlate to seven fit counterparts. Serological microRNAs were extracted using the miRNeasy Mini Kit(r) (Qiagen, Hilden, Germany). Quantification of microRNAs was performed using TaqMan Master Mix(r) reagent (Applied Biosystems, USA).
  
  
  RESULTS
  We obtained serological underexpression microRNA-21, microRNA-34a, and microRNA-126 in CRC group. However, miRNAs serological values do not impact prognosis. Furthermore, miRNAs was not influenced by CEA values, TNM staging, and histological subtype.
  
  
  CONCLUSIONS
  Despite lower expression of miR-21, miR-34a and miR-126 in the CRC group, no association with poor prognosis was found.
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: Ana Luiza Normanha Ribeiro de
authors.initials: AL
authors.lastname: Almeida
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: Mário Vinícius Angelete Alvarez
authors.initials: MV
authors.lastname: Bernardes
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: Marley Ribeiro
authors.initials: MR
authors.lastname: Feitosa
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: Fernanda Maris
authors.initials: FM
authors.lastname: Peria
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: Daniela Pretti da Cunha
authors.initials: DP
authors.lastname: Tirapelli
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: José Joaquim Ribeiro da
authors.initials: JJ
authors.lastname: Rocha
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: Omar
authors.initials: O
authors.lastname: Feres
mesh_terms: D000236:Adenoma / Q000097:blood* / Q000235:genetics; D000367:Age Factors; D014408:Biomarkers, Tumor / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000097:blood; D002277:Carcinoma / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011379:Prognosis; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D012016:Reference Values
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; C527111:MIRN126 microRNA, human; C524940:MIRN21 microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs