title: Homologous transplantation with fresh frozen bone for dental implant placement can induce HLA sensitization: a preliminary study.
abstract: It has been related in orthopedic surgeries the HLA sensitization. Thus, we evaluate if the use of fresh-frozen homologous bone (FFHB) for dental implant placement induce anti-HLA sensitization. Six patients were treated with FFHB corticocancellous block grafts. After 6 months, bone biopsies were harvested during implant placement to allow histomorphometric analysis. Vital mineralized tissue (VMT), non-vital mineralized tissue (NVMT) and non-mineralized tissue (NMT) were quantified histomorphometrically. Peripheral blood was collected from the patients before FFHB placement and 6 months after the surgery for anti-HLA analysis. The histomorphometric analysis showed the presence of VMT, NVMT and NMT in 45.56 ± 15.72 %, 14.16 ± 13.39 % and 40.29 ± 12.60 %, respectively. The baseline and 6 months postoperative CTs revealed bone thickness in the order of 5.66 ± 0.67 mm and 8.71 ± 1.52 mm (3.05 ± 1.39 mm). The anti-HLA analysis revealed that two of the six patients (33.3 %) became sensitized, however this was not associated with any FFHB incorporation loss (p > 0.05). A total of 24 implants were placed all of which were osseointegrated after 6 months. Although FFHB-related HLA sensitization does not appear to affect bone incorporation when treating insufficient bone thickness for implant placement, further follow-up is required to determine whether there is an association between HLA sensitization and long-term graft survival.
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, R. José Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil.
authors.forename: Paulo Eduardo
authors.initials: PE
authors.lastname: de Lacerda
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, R. José Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil.
authors.forename: André Antonio
authors.initials: AA
authors.lastname: Pelegrine
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, R. José Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil.
authors.forename: Marcelo Lucchesi
authors.initials: ML
authors.lastname: Teixeira
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, R. José Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil.
authors.forename: Victor Angelo Martins
authors.initials: VA
authors.lastname: Montalli
authors.affiliation: Laboratory of Transplantation Immunology, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Helcio
authors.initials: H
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, R. José Rocha Junqueira, 13, Campinas, SP, 13045-755, Brazil. marcelo.napimoga@gmail.com.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
mesh_terms: D001863:Bone Screws; D016025:Bone Transplantation; D015921:Dental Implants; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D014025:Titanium / Q000494:pharmacology; D014057:Tomography, X-Ray Computed; D014184:Transplantation, Homologous
chemical_list: D015921:Dental Implants; D006680:HLA Antigens; D014025:Titanium
keywords: Bone regeneration; Bone transplantation; Dental implant; HLA antigens; Homologous