title: Increased accumulation of polyhydroxybutyrate in divergent cyanobacteria under nutrient-deprived photoautotrophy: An efficient conversion of solar energy and carbon dioxide to polyhydroxybutyrate by Calothrix scytonemicola TISTR 8095.
abstract: The cellular PHB content was determined in 137 strains of cyanobacteria representing 88 species in 26 genera under six photoautotrophic nutrient conditions. One hundred and thirty-four strains were PHB producers. The PHB contents of these 134 strains were subtle under normal growth condition, but were significantly increased in 63 strains under nitrogen deprivation (-N), a higher frequency than with phosphate and/or potassium and all-nutrient deprivation. A high PHB accumulation was not associated with any particular evolutionary groups, but was strain specific. The filamentous Calothrix scytonemicola TISTR 8095 produced 356.5±63.4mg/L PHB under -N from a biomass of 1396.6±66.1mg/L, giving a PHB content of 25.4±3.5% (w/w dry weight). This PHB productivity is equivalent to the CO2 consumption of 729.2±129.8mg/L. The maximum energy conversion from solar energy to PHB obtained by C. scytonemicola TISTR 8095 was 1.42±0.30%.
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Auratai
authors.initials: A
authors.lastname: Kaewbai-Ngam
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Aran
authors.initials: A
authors.lastname: Incharoensakdi
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: tanakarn.m@chula.ac.th.
authors.forename: Tanakarn
authors.initials: T
authors.lastname: Monshupanee
mesh_terms: D052818:Autotrophic Processes; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D000458:Cyanobacteria / Q000254:growth & development / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012993:Solar Energy; D000094122:Polyhydroxybutyrates
chemical_list: D002245:Carbon Dioxide; D006885:Hydroxybutyrates; D009584:Nitrogen; D010710:Phosphates; D011091:Polyesters; D011188:Potassium; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Carbon dioxide; Cyanobacteria; Energy; PHB; Polyhydroxybutyrate