title: Peroxins Pex30 and Pex29 Dynamically Associate with Reticulons to Regulate Peroxisome Biogenesis from the Endoplasmic Reticulum.
abstract: Peroxisome proliferation occurs by at least two routes, division of existing peroxisomes and de novo biogenesis from the endoplasmic reticulum (ER). The proteins and molecular mechanisms governing peroxisome emergence from the ER are poorly characterized. In this study, we report that two integral membrane peroxins (proteins required for peroxisome biogenesis) in Saccharomyces cerevisiae, Pex29 and Pex30, reside in distinct regions of the ER and associate with Rtn1 and Yop1, reticulon family members that contribute to ER morphology, to govern peroxisome emergence from the ER. In vivo and in vitro analyses reveal that peroxisome proliferation is therefore not restricted to the peroxisome but begins at the level of the ER.
authors.affiliation: From the Center for Infectious Disease Research and Institute for Systems Biology, Seattle, Washington 98109 and.
authors.forename: Fred D
authors.identifier: 0000-0002-2177-6647
authors.initials: FD
authors.lastname: Mast
authors.affiliation: From the Center for Infectious Disease Research and Institute for Systems Biology, Seattle, Washington 98109 and.
authors.forename: Arvind
authors.initials: A
authors.lastname: Jamakhandi
authors.affiliation: From the Center for Infectious Disease Research and Institute for Systems Biology, Seattle, Washington 98109 and.
authors.forename: Ramsey A
authors.initials: RA
authors.lastname: Saleem
authors.affiliation: From the Center for Infectious Disease Research and Institute for Systems Biology, Seattle, Washington 98109 and.
authors.forename: David J
authors.initials: DJ
authors.lastname: Dilworth
authors.affiliation: From the Center for Infectious Disease Research and Institute for Systems Biology, Seattle, Washington 98109 and.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Rogers
authors.affiliation: the Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Rachubinski
authors.affiliation: From the Center for Infectious Disease Research and Institute for Systems Biology, Seattle, Washington 98109 and john.aitchison@CIDResearch.org.
authors.forename: John D
authors.initials: JD
authors.lastname: Aitchison
mesh_terms: D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C474735:PEX29 protein, S cerevisiae; C511360:Pex30 protein, S cerevisiae; C505725:Rtn1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C426246:YOP1 protein, S cerevisiae
keywords: endoplasmic reticulum (ER); microscopy; peroxin; peroxisome; peroxisomes; reticulon; subcellular fractionation; subcellular organelle; yeast