title: Prostaglandin-E1 has a protective effect on renal ischemia/reperfusion-induced oxidative stress and inflammation mediated gastric damage in rats.
abstract: Gastrointestinal complications are frequent in renal transplant recipients. In this regard, renal ischemia/reperfusion injury (IRI)-induced gastric damage seems to be important and there is no data available on the mechanism of this pathology. Because of its anti-inflammatory and anti-oxidant properties, it can be suggested that prostaglandin-E1 (PGE1) protects cells from renal IRI-induced gastric damage. The aim of this study was to investigate the molecular mechanisms of gastric damage induced by renal IRI and the effect of PGE1 on these mechanisms. We set an experiment with four different animal groups: physiological saline-injected and sham-operated rats, PGE1 (20μg/kg)-administered and sham operated rats, renal IRI subjected rats, and PGE1-administered and renal IRI subjected rats. The protective effect of PGE1 on renal IRI-induced gastric damage was determined based on reduced histological damage and lactate dehydrogenase activity. Moreover, we demonstrated that PGE1 shows its protective effect through reducing the production of reactive oxygen species and malondialdehyde levels. During histological examination, we observed the presence of common mononuclear cell infiltration. Therefore, pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β levels were measured and it has been shown that PGE1 suppressed both cytokines. Furthermore, it was found that PGE1 reduced the number of NF-κB(+) and caspase-3(+) inflammatory cells, and also NF-κB DNA-binding activity, while increasing proliferating cell nuclear antigen(+) epithelial cells in the stomach tissue of rats subjected to renal IR. Our data showed that PGE1 has a protective effect on renal IRI-induced oxidative stress and inflammation mediated gastric damage in rats.
authors.affiliation: Istanbul University, Faculty of Science, Department of Biology, 34134, Vezneciler, Istanbul, Turkey. Electronic address: selgez@istanbul.edu.tr.
authors.forename: Selda
authors.initials: S
authors.lastname: Gezginci-Oktayoglu
authors.affiliation: Istanbul University, Institute of Experimental Medicine, Department of Neuroscience, 34093, Capa, Istanbul, Turkey.
authors.forename: Nurcan
authors.initials: N
authors.lastname: Orhan
authors.affiliation: Istanbul University, Faculty of Science, Department of Biology, 34134, Vezneciler, Istanbul, Turkey.
authors.forename: Sehnaz
authors.initials: S
authors.lastname: Bolkent
mesh_terms: D000527:Alprostadil / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D005756:Gastritis / Q000209:etiology / Q000517:prevention & control*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D016030:Kidney Transplantation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D013270:Stomach / Q000187:drug effects* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; C000594089:IL1B protein, rat; D053583:Interleukin-1beta; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase; D000527:Alprostadil
keywords: Gastric damage; Inflammation; Oxidative stress; Prostaglandin-E(1); Renal ischemia/reperfusion injury