title: Development and validation of glycoprotein-based native-subunit vaccine for fish against Aeromonas hydrophila.
abstract: Aeromonas hydrophila is known to be causative agent of an infection named as Bacterial haemorrhagic septicaemia or red pest in freshwater fish. The aim of this study was to develop and validate the glycoprotein-based fish vaccine against Aeromonas hydrophila. For this aim, after identification and characterization of A. hydrophila isolates from fish farms, one A. hydrophila isolate was selected as vaccine strain. Antigenic glycoproteins of this vaccine strain were determined by Western blotting and glycan detection kit. The connection types of these glycoproteins were examined by glycoprotein differentiation kit. Two glycoproteins, molecular weights of 19 and 38 kDa, with SNA connection type were selected for use in vaccination trials. After their purification by SNA-specific lectin and size-exclusion chromatography, protection studies with purified proteins were performed. For challenge trials, four experimental fish groups were designated: Group I (with montanide), Group II (with montanide and ginseng), Group III [with Al(OH)3 ] and Group IV [with Al(OH)3 and ginseng]. The survival ratings of fish were determined, and protection was calculated as 21.56%, 29.41%, 69.83% and 78.88% in groups I, II, III and IV, respectively. In conclusion, A. hydrophila glycoproteins with Al(OH)3 and ginseng could be used as a safe and effective vaccine for fish.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Çiftci
authors.affiliation: Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
authors.forename: E E
authors.initials: EE
authors.lastname: Onuk
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Çiftci
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Fındık
authors.affiliation: High School of Health, University of Ondokuz Mayıs, Samsun, Turkey.
authors.forename: M Ü
authors.initials: MÜ
authors.lastname: Söğüt
authors.affiliation: Egirdir Fisheries Faculty, Suleyman Demirel University, Egirdir, Isparta, Turkey.
authors.forename: B I
authors.initials: BI
authors.lastname: Didinen
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Aksoy
authors.affiliation: Samsun Veterinary Control and Research Institute, Samsun, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Üstünakın
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Gülhan
authors.affiliation: Department of Aquaculture, Faculty of Fisheries, Recep Tayyip Erdoğan University, Rize, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Balta
authors.affiliation: Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Altun
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000627:therapeutic use; D001428:Bacterial Vaccines / Q000276:immunology* / Q000592:standards*; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control*; D006023:Glycoproteins / Q000276:immunology* / Q000627:therapeutic use; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D017686:Oncorhynchus mykiss; D014611:Vaccination / Q000662:veterinary*; D022223:Vaccines, Subunit / Q000276:immunology / Q000592:standards
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006023:Glycoproteins; D022223:Vaccines, Subunit
keywords: Aeromonas hydrophila; glycoprotein; vaccine