title: Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes.
abstract: Dendritic cells (DCs) are a rare population of antigen-presenting cells that initiate immune responses in secondary lymphoid organs. In order to better understand the properties of DC in humans, it is essential to analyze DC subsets directly purified from tissues. Here, we describe a protocol allowing the purification of DC subsets from human tonsils and human lymph nodes.
authors.affiliation: Institut Curie, Centre de recherche, 26 rue d'Ulm, 75248, Paris Cedex 05, France.|INSERM U932, 26 rue d'Ulm, 75248, Paris Cedex 05, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Durand
authors.affiliation: Institut Curie, Centre de recherche, 26 rue d'Ulm, 75248, Paris Cedex 05, France. elodie.segura@curie.fr.|INSERM U932, 26 rue d'Ulm, 75248, Paris Cedex 05, France. elodie.segura@curie.fr.
authors.forename: Elodie
authors.initials: E
authors.lastname: Segura
mesh_terms: D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology*; D014066:Palatine Tonsil / Q000166:cytology*
keywords: DC subsets; Dendritic cells; Human; Lymph node; Tonsil