title: Endophytic Actinobacteria from the Brazilian Medicinal Plant Lychnophora ericoides Mart. and the Biological Potential of Their Secondary Metabolites.
abstract: Endophytic actinobacteria from the Brazilian medicinal plant Lychnophora ericoides were isolated for the first time, and the biological potential of their secondary metabolites was evaluated. A phylogenic analysis of isolated actinobacteria was accomplished with 16S rRNA gene sequencing, and the predominance of the genus Streptomyces was observed. All strains were cultured on solid rice medium, and ethanol extracts were evaluated with antimicrobial and cytotoxic assays against cancer cell lines. As a result, 92% of the extracts showed a high or moderate activity against at least one pathogenic microbial strain or cancer cell line. Based on the biological and chemical analyses of crude extracts, three endophytic strains were selected for further investigation of their chemical profiles. Sixteen compounds were isolated, and 3-hydroxy-4-methoxybenzamide (9) and 2,3-dihydro-2,2-dimethyl-4(1H)-quinazolinone (15) are reported as natural products for the first time in this study. The biological activity of the pure compounds was also assessed. Compound 15 displayed potent cytotoxic activity against all four tested cancer cell lines. Nocardamine (2) was only moderately active against two cancer cell lines but showed strong activity against Trypanosoma cruzi. Our results show that endophytic actinobacteria from L. ericoides are a promising source of bioactive compounds.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, 14040-903, Ribeirão Preto, SP, Brazil.|CEPID-CIBFar - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, FAPESP, São Paulo, SP, Brazil.
authors.forename: Raphael
authors.initials: R
authors.lastname: Conti
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, 14040-903, Ribeirão Preto, SP, Brazil.|CEPID-CIBFar - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, FAPESP, São Paulo, SP, Brazil.
authors.forename: Fernanda Oliveira
authors.initials: FO
authors.lastname: Chagas
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, 14040-903, Ribeirão Preto, SP, Brazil.|CEPID-CIBFar - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, FAPESP, São Paulo, SP, Brazil.
authors.forename: Andrés Mauricio
authors.initials: AM
authors.lastname: Caraballo-Rodriguez
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, 14040-903, Ribeirão Preto, SP, Brazil.|CEPID-CIBFar - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, FAPESP, São Paulo, SP, Brazil.
authors.forename: Weilan Gomes da Paixão
authors.initials: WG
authors.lastname: Melo
authors.affiliation: Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Andréa Mendes
authors.initials: AM
authors.lastname: do Nascimento
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Bruno Coêlho
authors.initials: BC
authors.lastname: Cavalcanti
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Manoel Odorico
authors.initials: MO
authors.lastname: de Moraes
authors.affiliation: Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil.|Fundação Oswaldo Cruz (Fiocruz), Fortaleza, CE, Brazil.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Pessoa
authors.affiliation: Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Letícia Veras
authors.initials: LV
authors.lastname: Costa-Lotufo
authors.affiliation: CEPID-CIBFar - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, FAPESP, São Paulo, SP, Brazil.|Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Krogh
authors.affiliation: CEPID-CIBFar - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, FAPESP, São Paulo, SP, Brazil.|Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Adriano Defini
authors.initials: AD
authors.lastname: Andricopulo
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, 14040-903, Ribeirão Preto, SP, Brazil.
authors.forename: Norberto Peporine
authors.initials: NP
authors.lastname: Lopes
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n, 14040-903, Ribeirão Preto, SP, Brazil. mtpupo@fcfrp.usp.br.|CEPID-CIBFar - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos, FAPESP, São Paulo, SP, Brazil. mtpupo@fcfrp.usp.br.
authors.forename: Mônica Tallarico
authors.initials: MT
authors.lastname: Pupo
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000382:microbiology*; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D021261:Parasitic Sensitivity Tests; D010946:Plants, Medicinal / Q000382:microbiology; D064210:Secondary Metabolism; D013329:Structure-Activity Relationship; D014349:Trypanosoma cruzi / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000981:Antiprotozoal Agents; D001688:Biological Products
keywords: Actinobacteria; Biological activity; Endophytes; Lychnophora ericoides; Natural products