title: Mouse JMJD4 is dispensable for embryogenesis.
abstract: Jumonji C domain-containing demethylase 4 (JMJD4) is thought to help regulate mRNA translation, yet its precise in vivo role during mouse development has not been addressed. In the present study, we examined the contribution of this demethylase to embryonic stem cell (ESC) differentiation, and established a Jmjd4-knockout mouse to explore its role during embryonic development. Jmjd4 expression is diminished upon ESC differentiation, and becomes restricted to certain developing organs, such as the eyes and gut, in embryonic Day-11.5 embryos. Unexpectedly, Jmjd4-null ESCs exhibited normal colony morphology and maintained normal expression of pluripotent genes. Furthermore, Jmjd4-knockout embryos are born at a normal Mendelian ratio. Thus, JMJD4 is dispensable in murine development. Mol. Reprod. Dev. 83: 588-593, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Nanobiomedical Science, BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan-si, Republic of Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Yoo
authors.affiliation: Department of Nanobiomedical Science, BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan-si, Republic of Korea.
authors.forename: Dabin
authors.initials: D
authors.lastname: Son
authors.affiliation: Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Republic of Korea.
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Nanobiomedical Science, BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan-si, Republic of Korea.
authors.forename: Kwonho
authors.initials: K
authors.lastname: Hong
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D004622:Embryo, Mammalian / Q000201:enzymology*; D047108:Embryonic Development / Q000502:physiology*; D056484:Jumonji Domain-Containing Histone Demethylases; D051379:Mice; D018345:Mice, Knockout; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000201:enzymology*; D038081:Organogenesis / Q000502:physiology*; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000235:genetics / Q000378:metabolism*
chemical_list: D056484:Jumonji Domain-Containing Histone Demethylases; C000610409:JMJD4 protein, mouse; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase