title: Anticoagulant properties and cytotoxic effect against HCT116 human colon cell line of sulfated glycosaminoglycans isolated from the Norway lobster (Nephrops norvegicus) shell.
abstract: Sulfated glycosaminoglycans (SGNL) were extracted for the first time from Norway lobster (Nephrops norvegicus) shell. The monosaccharide composition analysed by GC/MS revealed the presence of galacturonic acid, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine. The analysis of SGNL with acetate cellulose electrophoresis in Zn-acetate revealed the presence of heparan sulfate (HS) and dermatan sulfate (DS). SGNL were evaluated for their anticoagulant activities using activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. After 21h incubation, HCT116 cell proliferation was inhibited (p<0.05) between 39.7 and 54.8% at 1.5-7.5mg/mL of SGNL. SGNL don't show hemolytic activity towards bovine erythrocytes and no cytotoxicity against the normal lymphocytes. The antiproliferative efficacy of these lobster glycosaminoglycans were probably related with the higher sulfate content. SGNL demonstrated promising antiproliferative and anticoagulant potential, which may be used as a novel, effective and promising antithrombotic agent.
authors.affiliation: Unité Enzymes et Bioconversion, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Km 4 Road Soukra, 3038 Sfax, Tunisia.
authors.forename: Nadhem
authors.initials: N
authors.lastname: Sayari
authors.affiliation: Unité Enzymes et Bioconversion, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Km 4 Road Soukra, 3038 Sfax, Tunisia.
authors.forename: Rafik
authors.initials: R
authors.lastname: Balti
authors.affiliation: Laboratoire de Génétique, Biodiversité et Valorisation des Bioressources (LR 11-ES 41), University of Monastir, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Mansour
authors.affiliation: Centre Régional de Transfusion Sanguine de Sfax, Route El-Ain Km 0.5, CP 3003 Sfax, Tunisia.
authors.forename: Ikram
authors.initials: I
authors.lastname: Ben Amor
authors.affiliation: Laboratoire de recherche sur les substances biologiquement compatibles, Faculté de médecine dentaire, Rue Avicenne, 5019 Monastir, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Graiet
authors.affiliation: Centre Régional de Transfusion Sanguine de Sfax, Route El-Ain Km 0.5, CP 3003 Sfax, Tunisia.
authors.forename: Jalel
authors.initials: J
authors.lastname: Gargouri
authors.affiliation: Unité Enzymes et Bioconversion, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Km 4 Road Soukra, 3038 Sfax, Tunisia. Electronic address: ali.bougatef79@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Bougatef
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003106:Colon / Q000473:pathology*; D004586:Electrophoresis; D006025:Glycosaminoglycans / Q000302:isolation & purification* / Q000494:pharmacology*; D045325:HCT116 Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008121:Nephropidae / Q000737:chemistry*; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000925:Anticoagulants; D006025:Glycosaminoglycans; C013786:A73025
keywords: Anticoagulant properties; Antiproliferative activity; Chemical characterization; Nephrops norvegicus; Sulfated glycosaminoglycans