title: Acute effects of sono-activated photocatalytic titanium dioxide nanoparticles on oral squamous cell carcinoma.
abstract: Sonodynamic therapy (SDT) is a new treatment modality using ultrasound to activate certain chemical sensitizers for cancer therapy. In this study, effects of high intensity focused ultrasound (HIFU) combined with photocatalytic titanium dioxide (TiO2) nanoparticles on human oral squamous cell line HSC-2 were investigated. Viability of HSC-2 cells after 0, 0.1, 1, or 3s of HIFU irradiation with 20, 32, 55 and 73Wcm(-2) intensities in the presence or absence of TiO2 was measured immediately after the exposures in vitro. Immediate effects of HIFU (3s, 73Wcm(-2)) combined with TiO2 on solid tumors were also examined by histological study. Cytotoxic effect of HIFU+TiO2in vitro was significantly higher than that of TiO2 or HIFU alone with the tendency to increase for higher HIFU intensity, duration, and TiO2 concentration in the suspension. In vivo results showed significant necrosis and tissue damage in HIFU and HIFU+TiO2 treated samples. However, penetration of TiO2 nanoparticles into the cell cytoplasm was only observed in HIFU+TiO2 treated tissues. In this study, our findings provide a rational basis for the development of an effective HIFU based sonodynamic activation method. This approach offers an attractive non-invasive therapy technique for oral cancer in future.
authors.affiliation: Department of Anatomy, School of Medicine, Fukuoka University, Fukuoka, Japan. Electronic address: Moosavi@fukuoka-u.ac.jp.
authors.forename: S
authors.initials: S
authors.lastname: Moosavi Nejad
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Bioelectrics Department, Institute of Pulsed Power Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Hamid
authors.initials: H
authors.lastname: Hosseini
authors.affiliation: Department of Anatomy, School of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Watanabe
authors.affiliation: Department of Anatomy, School of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Endo
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Narihira
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kikuta
authors.affiliation: Department of Anatomy, School of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Katsuro
authors.initials: K
authors.lastname: Tachibana
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D002470:Cell Survival; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy*; D053758:Nanoparticles / Q000627:therapeutic use*; D014025:Titanium / Q000494:pharmacology*
chemical_list: C009495:titanium dioxide; D014025:Titanium
keywords: High intensity focused ultrasound (HIFU); Oral squamous cell carcinoma; Photocatalytic titanium dioxide (TiO(2)) nanoparticles; Sonodynamic therapy