title: Development of antiviral gene therapy for Monodon baculovirus using dsRNA loaded chitosan-dextran sulfate nanocapsule delivery system in Penaeus monodon post-larvae.
abstract: In the present study, a suitable carrier system was developed for the delivery of dsRNA into Penaeus monodon (P. monodon) post larvae to silence the Monodon baculovirus (MBV) structural gene of p74. The carrier system was developed by layer by layer adsorption of oppositely charged chitosan-dextran sulfate, on charged silica nanoparticles. The silica template was removedto produce multilayered hollow nanocapsules (CS-DS) that were utilized for dsRNA loading at an alkaline pH. The capsule's surface was modified by conjugating with shrimp feed for enhanced cellular uptake. In vivo cellular uptake of CS-DS/FITC loaded nanocapsules conjugated with feed was studied after oral administration into post-larvae. The results revealed that the encapsulated FITC was effectively delivered and exhibited a sustained release into the cytoplasm of shrimp post-larvae. The MBV challenge study for structural gene p74was conducted after 3-25 days of post infection (dpi) with respective CS-DS/dsRNA coated with feed. The results showed a significant survival rate of 86.63% and effective gene silencing in P. monodon. Our findings indicated that the delivery of dsRNA using shrimp feed coatedCS-DSnanocapsules could be a novel approach to prevent viral infections in shrimp.
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
authors.forename: D
authors.initials: D
authors.lastname: Ramesh Kumar
authors.affiliation: Department of Materials Engineering, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: Rajasegaran
authors.initials: R
authors.lastname: Elumalai
authors.affiliation: Department of Materials Engineering, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: Ashok M
authors.initials: AM
authors.lastname: Raichur
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
authors.forename: M
authors.initials: M
authors.lastname: Sanjuktha
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
authors.forename: J J
authors.initials: JJ
authors.lastname: Rajan
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Alavandi
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Vijayan
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
authors.forename: M
authors.initials: M
authors.lastname: Poornima
authors.affiliation: Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture, Chennai 600028, India; Entomology Research Institute, Loyola College, Nungambakkam, Chennai 600 034, India. Electronic address: santiagotc1@yahoo.co.in.
authors.forename: T C
authors.initials: TC
authors.lastname: Santiago
mesh_terms: D000821:Animal Feed; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000737:chemistry; D016367:Baculoviridae / Q000235:genetics*; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry; D016264:Dextran Sulfate; D020868:Gene Silencing; D015316:Genetic Therapy; D007814:Larva / Q000821:virology; D053769:Nanocapsules / Q000737:chemistry; D033561:Penaeidae / Q000821:virology*; D012330:RNA, Double-Stranded / Q000008:administration & dosage*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000998:Antiviral Agents; D053769:Nanocapsules; D012330:RNA, Double-Stranded; C480355:chitosan sulfate; D012822:Silicon Dioxide; D048271:Chitosan; D016264:Dextran Sulfate
keywords: Chitosan-dextran sulfate nanocapsules; Gene silencing; MBV; Penaeus monodon; dsRNA delivery