title: Can Changes in Temperature or Ionic Conditions Modify the DNA Organization in the Full Bacteriophage Capsid?
abstract: We compared four bacteriophage species, T5, λ, T7, and Φ29, to explore the possibilities of DNA reorganization in the capsid where the chain is highly concentrated and confined. First, we did not detect any change in DNA organization as a function of temperature between 20 to 40 °C. Second, the presence of spermine (4+) induces a significant enlargement of the typical size of the hexagonal domains in all phages. We interpret these changes as a reorganization of DNA by slight movements of defects in the structure, triggered by a partial screening of repulsive interactions. We did not detect any signal characteristic of a long-range chiral organization of the encapsidated DNA in the presence and in the absence of spermine.
authors.affiliation: Laboratoire de Physique des Solides, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91405 Orsay Cedex, France.
authors.forename: Marta de
authors.initials: Md
authors.lastname: Frutos
authors.affiliation: Laboratoire de Physique des Solides, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91405 Orsay Cedex, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Leforestier
authors.affiliation: Laboratoire de Physique des Solides, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91405 Orsay Cedex, France.
authors.forename: Jéril
authors.initials: J
authors.lastname: Degrouard
authors.affiliation: Laboratoire de Physique des Solides, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91405 Orsay Cedex, France.
authors.forename: Nebraska
authors.initials: N
authors.lastname: Zambrano
authors.affiliation: Synchrotron SOLEIL, DISCO, L'Orme des Merisiers , 91190 St Aubin, France.
authors.forename: Frank
authors.initials: F
authors.lastname: Wien
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS UMR 9198, Univ. Paris-Sud, Université Paris-Saclay , 91198 Gif sur Yvette Cedex, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Boulanger
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS UMR 9198, Univ. Paris-Sud, Université Paris-Saclay , 91198 Gif sur Yvette Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Brasilès
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS UMR 9198, Univ. Paris-Sud, Université Paris-Saclay , 91198 Gif sur Yvette Cedex, France.
authors.forename: Madalena
authors.initials: M
authors.lastname: Renouard
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS UMR 9198, Univ. Paris-Sud, Université Paris-Saclay , 91198 Gif sur Yvette Cedex, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Durand
authors.affiliation: Laboratoire de Physique des Solides, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91405 Orsay Cedex, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Livolant
mesh_terms: D017123:Bacteriophage T7 / Q000737:chemistry* / Q000648:ultrastructure; D010582:Bacteriophage lambda / Q000737:chemistry* / Q000648:ultrastructure; D002213:Capsid / Q000737:chemistry* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D042003:DNA Packaging; D004279:DNA, Viral / Q000737:chemistry* / Q000648:ultrastructure; D009690:Nucleic Acid Conformation; D017906:Siphoviridae / Q000737:chemistry* / Q000648:ultrastructure; D013045:Species Specificity; D013096:Spermine / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D004279:DNA, Viral; D013096:Spermine