title: CD55 is a key complement regulatory protein that counteracts complement-mediated inactivation of Newcastle Disease Virus.
abstract: Newcastle disease virus (NDV) is being developed as an oncolytic virus for virotherapy. In this study we analysed the regulation of complement-mediated inactivation of a recombinant NDV in different host cells. NDV grown in human cells was less sensitive to complement-mediated virus inactivation than NDV grown in embryonated chicken eggs. Additionally, NDV produced from HeLa-S3 cells is more resistant to complement than NDV from 293F cells, which correlated with higher expression and incorporation of complement regulatory proteins (CD46, CD55 and CD59) into virions from HeLa-S3 cells. Further analysis of the recombinant NDVs individually expressing the three CD molecules showed that CD55 is the most potent in counteracting complement-mediated virus inactivation. The results provide important information on selecting NDV manufacture substrate to mitigate complement-mediated virus inactivation.
authors.affiliation: MedImmune LLC, Mountain View, California, USA.
authors.forename: Udaya S
authors.initials: US
authors.lastname: Rangaswamy
authors.affiliation: MedImmune LLC, Mountain View, California, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Cotter
authors.affiliation: MedImmune LLC, Mountain View, California, USA.
authors.forename: Xing
authors.initials: X
authors.lastname: Cheng
authors.affiliation: MedImmune LLC, Mountain View, California, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.affiliation: MedImmune LLC, Mountain View, California, USA.
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D018958:CD55 Antigens / Q000378:metabolism*; D018957:CD59 Antigens / Q000378:metabolism; D002460:Cell Line; D002645:Chickens; D003169:Complement Inactivator Proteins / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism*; D051916:Membrane Cofactor Protein / Q000378:metabolism; D009522:Newcastle disease virus / Q000276:immunology* / Q000502:physiology*
chemical_list: D018958:CD55 Antigens; D018957:CD59 Antigens; D003169:Complement Inactivator Proteins; D007155:Immunologic Factors; D051916:Membrane Cofactor Protein; D003165:Complement System Proteins