title: Identification and Comparative Analysis of CBS Domain-Containing Proteins in Soybean (Glycine max) and the Primary Function of GmCBS21 in Enhanced Tolerance to Low Nitrogen Stress.
abstract: Nitrogen is an important macronutrient required for plant growth, and is a limiting factor for crop productivity. Improving the nitrogen use efficiency (NUE) is therefore crucial. At present, the NUE mechanism is unclear and information on the genes associated with NUE in soybeans is lacking. cystathionine beta synthase (CBS) domain-containing proteins (CDCPs) may be implicated in abiotic stress tolerance in plants. We identified and classified a CBS domain-containing protein superfamily in soybean. A candidate gene for NUE, GmCBS21, was identified. GmCBS21 gene characteristics, the temporal expression pattern of the GmCBS21 gene, and the phenotype of GmCBS21 overexpression in transgenic Arabidopsis thaliana under low nitrogen stress were analyzed. The phenotypes suggested that the transgenic Arabidopsis thaliana seedlings performed better under the nitrogen-deficient condition. GmCBS21-overexpressing transgenic plants exhibit higher low nitrogen stress tolerance than WT plants, and this suggests its role in low nitrogen stress tolerance in plants. We conclude that GmCBS21 may serve as an excellent candidate for breeding crops with enhanced NUE and better yield.
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China. haoqingnan@126.com.|Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. haoqingnan@126.com.
authors.forename: Qingnan
authors.initials: Q
authors.lastname: Hao
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China. shang-weijuan2006@163.com.|Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. shang-weijuan2006@163.com.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Shang
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China. zhangchanjuan@aliyun.com.|Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. zhangchanjuan@aliyun.com.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China. smartchf@163.com.|Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. smartchf@163.com.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China. ccllmm_008@163.com.|Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. ccllmm_008@163.com.
authors.forename: Limiao
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China. yyyyy-0909@163.com.|Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. yyyyy-0909@163.com.
authors.forename: Songli
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. chenshuilianjy@163.com.
authors.forename: Shuilian
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. xjzhang@oilcrops.cn.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China. zhouxinan@caas.cn.|Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan 430062, China. zhouxinan@caas.cn.
authors.forename: Xinan
authors.initials: X
authors.lastname: Zhou
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism; D003541:Cystathionine beta-Synthase / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D030541:Databases, Genetic; D009584:Nitrogen / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D000072417:Protein Domains; D036226:Seedlings / Q000378:metabolism; D030262:Soybean Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D030262:Soybean Proteins; D003541:Cystathionine beta-Synthase; D009584:Nitrogen
keywords: CBS domain; function analysis; low nitrogen stress tolerance; soybean