title: Processing of AtBAG6 triggers autophagy and fungal resistance.
abstract: The Bcl-2-associated athanogene (BAG) family is an evolutionarily conserved, multifunctional group of cytoprotective co-chaperones. Using structural bioinformatic approaches we identified 7 homologs of the Arabidopsis BAG family. Evaluating knockouts in Arabidopsis of individual BAG family members, we noted that Arabidopsis BAG6 (AtBAG6) knockout lines exhibited a pronounced enhancement of susceptibility to the necrotrophic fungal pathogen Botrytis cinerea. Moreover, we identified a single predicted caspase-1 site that was cleaved by an aspartyl protease (AtAPCB1). Finally, we showed AtBAG6 forms a complex with AtAPCB1 via coupling to a C2 GRAM domain protein (AtBAGP1). This complex and its activation is necessary for triggering pathogen mediated autophagic cell death and host resistance.
authors.affiliation: a Institute for Plant Genomics and Biotechnology, Department of Plant Pathology and Microbiology, Texas A&M University , College Station , TX , USA.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Institute for Plant Genomics and Biotechnology, Department of Plant Pathology and Microbiology, Texas A&M University , College Station , TX , USA.
authors.forename: Marty
authors.initials: M
authors.lastname: Dickman
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001343:Autophagy; D020171:Botrytis; D060467:Disease Resistance; D008954:Models, Biological; D018832:Molecular Chaperones / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D011523:Protoplasts / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000611886:BAG6 protein, Arabidopsis; D018832:Molecular Chaperones; D009687:Nuclear Proteins
keywords: AtBAG6; Autophagy; C2-GRAM domain protein; aspartyl protease; fungal resistance; programmed cell death