title: MMP-3-1612 polymorphism - a risk factor for deep venous thrombosis formation.
abstract: We investigated the association of the 5A/6A polymorphism in the promoter region at -1612 of the matrix metalloproteinase-3 gene (MMP-3-1612) and deep venous thrombosis (DVT).
  
  
  
  The distribution of the MMP-3 (-1612 5A/6A) polymorphism in the case and control groups was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum MMP-3 level of two groups was detected using enzyme-linked immunosorbent assay (ELISA). HepG2 cells containing MMP-3-1612 recombinant plasmid were cultured in vitro and the MMP-3 level was defined by luminescence intensity of luciferase. A DVT rat model was built. Serum MMP-3 level in the rats' wounded vein at different time points was detected by ELISA and recorded for investigation of the association between MMP-3 and DVT. Statistical data analysis was conducted with SPSS18.0.
  
  
  
  On the basis of the observation of MMP-3-1612 genotype frequency and allele frequency in the case and control groups, we identified significantly higher MMP-3-1612 5A allele frequency and higher serum MMP-3 level in the case group than in the control group (both P < 0.05). According to in vitro luciferase measurements, the 5A allele had higher transcriptional activity than the 6A allele. As observed in the rat model, serum MMP-3 level increased with time passing and thrombosis formation after modelling.
  
  
  
  The MMP-3-1612 5A/6A polymorphism may effect serum MMP-3 level and over-expression of serum MMP-3 level may be a risk factor for DVT formation.
authors.affiliation: Department of Physiology, Binzhou Medical University, Yantai, P. R. China.
authors.forename: La-Mei
authors.initials: LM
authors.lastname: Yu
authors.affiliation: Department of Interventional Medicine, Affiliated Hospital of Binzhou Medical University, Binzhou, P. R. China.
authors.forename: Nai-Xuan
authors.initials: NX
authors.lastname: Li
authors.affiliation: Department of Interventional Medicine, Affiliated Hospital of Binzhou Medical University, Binzhou, P. R. China.
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Sheng
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D016022:Case-Control Studies; D004195:Disease Models, Animal; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D017207:Rats, Sprague-Dawley; D018570:Risk Assessment; D012307:Risk Factors; D014162:Transfection; D020246:Venous Thrombosis / Q000097:blood / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics*; D055815:Young Adult
chemical_list: C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3
keywords: MMP-3-1612; Matrix metalloproteinase-3; deep venous thrombosis; polymorphism; rat model; recombinant plasmid