title: Effects of exercise and bryostatin-1 on serotonin dynamics after cerebral infarction.
abstract: Although it has been suggested that the combination of exercise and bryostatin-1 administration may induce greater functional recovery than exercise alone, the detailed molecular mechanisms are not well known. Here, we examined the relationship between this combination treatment and monoamine dynamics in the cerebral cortex peri-infarction area to promote our understanding of these molecular mechanisms. Experimental cerebral cortex infarctions were produced by photothrombosis in rats. Voluntary exercise was initiated 2 days after surgery. Motor performance was then measured using the rotarod test. Monoamine concentrations in the perilesional cortex were analyzed by high-performance liquid chromatography. In behavioral evaluations, performance in the rotarod test was significantly increased by exercise. Moreover, performance in the rotarod test after the combination of exercise and bryostatin-1 administration was significantly greater than that after exercise alone. In the analysis of monoamines, serotonin (5-HT) concentrations were significantly higher in the groups treated with exercise and bryostatin-1. In addition, 5-HT turnover was significantly lower in the groups treated with exercise and bryostatin-1. Furthermore, the mean latency in the rotarod test showed a significant positive correlation with 5-HT levels. In immunohistochemical analysis, 5-HT immunoreactivity in the dorsal raphe nucleus was shown to be higher in the groups treated with exercise. In the present study, we detected changes in the levels of monoamines associated with the combined treatment of exercise and bryostatin-1 administration in the perilesional cortex. It has been suggested that this combination of therapies may affect 5-HT turnover and serve to increase local 5-HT concentrations in the perilesional area.
authors.affiliation: aDivision of Biochemistry, Fujita Memorial Nanakuri Institute bDepartment of Rehabilitation Medicine II, School of Medicine cDepartment of Internal Medicine, Nanakuri Sanatorium, Fujita Health University, Tsu dDepartment of Anatomy, Faculty of Medical Technology, School of Health Sciences, Fujita Health University, Toyoake, Japan.
authors.forename: Kenmei
authors.initials: K
authors.lastname: Mizutani
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sonoda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Wakita
authors.forename: Hideto
authors.initials: H
authors.lastname: Okazaki
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Katoh
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chihara
authors.forename: Kan
authors.initials: K
authors.lastname: Shimpo
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism; D054713:Bryostatins / Q000627:therapeutic use*; D002540:Cerebral Cortex / Q000378:metabolism; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000534:rehabilitation*; D004195:Disease Models, Animal; D005081:Exercise Therapy / Q000379:methods*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D045442:Rotarod Performance Test; D012701:Serotonin / Q000378:metabolism*
chemical_list: D000276:Adjuvants, Immunologic; D015306:Biogenic Monoamines; D054713:Bryostatins; D012701:Serotonin; C046785:bryostatin 1