title: Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits.
abstract: The Sleeping Beauty transposon system was established as a robust and efficient method for germline transgenesis in different mammalian species. The generation of transgenic mice, rats, rabbits and swine carrying an identical Venus reporter construct delivered by transposon-mediated gene transfer enables comparative studies of gene expression in these lines of mammalian models. Whereas comparable expression patterns of the Venus reporter were found in somatic tissues, preliminary studies suggested that a striking difference in reporter expression may exist in mature spermatozoa of these species. Here we clearly show the differential expression of Venus reporter protein during spermatogenesis of the two compared species, the laboratory rabbit and mice. We provide evidence for the functionality of intercellular bridges in the male germline and genotype-independent transgenic phenotype of rabbit spermatids. Our data suggest that the reporter rabbit line may be a suitable tool to identify molecular mechanisms in testicular development, and may contribute to develop better animal models for male infertility in men.
authors.affiliation: NARIC-Agricultural Biotechnology Institute, Gödöllő, Hungary.
authors.forename: Orsolya I
authors.initials: OI
authors.lastname: Hoffmann
authors.affiliation: NARIC-Agricultural Biotechnology Institute, Gödöllő, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Kerekes
authors.affiliation: NARIC-Agricultural Biotechnology Institute, Gödöllő, Hungary.
authors.forename: Nandor
authors.initials: N
authors.lastname: Lipták
authors.affiliation: NARIC-Agricultural Biotechnology Institute, Gödöllő, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Hiripi
authors.affiliation: NARIC-Agricultural Biotechnology Institute, Gödöllő, Hungary.
authors.forename: Szilard
authors.initials: S
authors.lastname: Bodo
authors.affiliation: Faculty of Medicine, University of Debrecen, Department of Biophysics and Cell Biology, Debrecen, Hungary.
authors.forename: Gabor
authors.initials: G
authors.lastname: Szaloki
authors.affiliation: Department of Biotechnology, Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Mariensee, Neustadt, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Klein
authors.affiliation: Paul-Ehrlich-Institut (PEI), Langen, Germany.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Ivics
authors.affiliation: Department of Biotechnology, Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Mariensee, Neustadt, Germany.
authors.forename: Wilfried A
authors.initials: WA
authors.lastname: Kues
authors.affiliation: NARIC-Agricultural Biotechnology Institute, Gödöllő, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bosze
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements; D005456:Fluorescent Dyes / Q000737:chemistry; D017930:Genes, Reporter; D005854:Germ Cells; D008297:Male; D011817:Rabbits; D013737:Testis / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D005456:Fluorescent Dyes