title: Thin bacteria/Layered Double Hydroxide films using a layer-by-layer approach.
abstract: This paper reports the design of thin bacteria/Layered Double Hydroxides (LDH) films in which bacterial cells of Pseudomonas sp. strain ADP were assembled alternatively with Mg2Al-NO3 LDH nanosheets by a layer-by-layer deposition method. The UV-Vis spectroscopy was used to monitor the assembly process, showing a progressive increase in immobilized bacteria amount upon deposited cycles. The {ADP/LDH}n film was characterized by X-ray diffraction, infrared spectroscopy, scanning electron microscopy and atomic force microscopy. The metabolic activity of immobilized bacteria was determined using chronoamperometry by measuring the biochemical oxygen demand in presence of glucose using an artificial electron acceptor (Fe(CN)6(3-)) at 0.5V/Ag-AgCl. A steady current of 0.250μAcm(-2) was reached in about 30s after the addition of 5mM glucose.
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63178 Aubiere, France.
authors.forename: Matilte
authors.initials: M
authors.lastname: Halma
authors.affiliation: Laboratoire de Physico-Chimie des Matériaux, Catalyse et Environnement Usto, El M'nouar, Oran, Algeria.
authors.forename: Aicha
authors.initials: A
authors.lastname: Khenifi
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63178 Aubiere, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Sancelme
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63178 Aubiere, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Besse-Hoggan
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63178 Aubiere, France.
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Bussière
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63178 Aubiere, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Prévot
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63178 Aubiere, France. Electronic address: Christine.Mousty@univ-bpclermont.fr.
authors.forename: Christine
authors.initials: C
authors.lastname: Mousty
mesh_terms: D006878:Hydroxides / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D009566:Nitrates / Q000737:chemistry; D010316:Particle Size; D011549:Pseudomonas / Q000737:chemistry* / Q000166:cytology; D013499:Surface Properties
chemical_list: D006878:Hydroxides; D009566:Nitrates; D008274:Magnesium
keywords: Bacteria; Bioelectrode; Layer-by-layer; Layered Double Hydroxides; Thin film