title: Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt-jakob disease: An international study.
abstract: Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT-QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160-165.
authors.affiliation: National CJD Research & Surveillance Unit, Western General Hospital, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
authors.forename: Lynne I
authors.initials: LI
authors.lastname: McGuire
authors.affiliation: Department of Neurological Sciences, National Institute of Health, Rome, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Poleggi
authors.affiliation: Institute of Neurological Sciences, Scientific Institute for Research, Hospitalization and Health Care, Bologna, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Poggiolini
authors.affiliation: Department of Neurodegenerative Diseases, Scientific Institute for Research, Hospitalization and Health Care, Carlo Besta Neurological Institute, Milan, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Suardi
authors.affiliation: Sorbonne Universities, Pierre and Marie Curie University, Brain and Spine Institute, Paris, France.|National Reference Centre for Unconventional Transmissible Agents, Paris, France.
authors.forename: Katarina
authors.initials: K
authors.lastname: Grznarova
authors.affiliation: Center for Neuropathology and Prion Research, Ludwig Maximilian University, Munich, Germany.
authors.forename: Song
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Neurology, Institute of Born Bunge, University of Antwerp, Antwerp, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: de Vil
authors.affiliation: Florey Institute of Neuroscience and Mental Health, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Shannon
authors.initials: S
authors.lastname: Sarros
authors.affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Satoh
authors.affiliation: Prion Laboratory Section, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Keding
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Neurology, University Medical Center and German Center for Neurodegenerative Diseases, University of Göttingen, Göttingen, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Cramm
authors.affiliation: National CJD Research & Surveillance Unit, Western General Hospital, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
authors.forename: Graham
authors.initials: G
authors.lastname: Fairfoul
authors.affiliation: Department of Neurology, University Medical Center and German Center for Neurodegenerative Diseases, University of Göttingen, Göttingen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Schmitz
authors.affiliation: Department of Neurology, University Medical Center and German Center for Neurodegenerative Diseases, University of Göttingen, Göttingen, Germany.
authors.forename: Inga
authors.initials: I
authors.lastname: Zerr
authors.affiliation: Department of Neurology, Institute of Born Bunge, University of Antwerp, Antwerp, Belgium.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cras
authors.affiliation: Department of Neurological Sciences, National Institute of Health, Rome, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Equestre
authors.affiliation: Department of Neurodegenerative Diseases, Scientific Institute for Research, Hospitalization and Health Care, Carlo Besta Neurological Institute, Milan, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Tagliavini
authors.affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Atarashi
authors.affiliation: Prion Laboratory Section, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Knox
authors.affiliation: Department of Medicine, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Steven
authors.initials: S
authors.lastname: Collins
authors.affiliation: Sorbonne Universities, Pierre and Marie Curie University, Brain and Spine Institute, Paris, France.|National Reference Centre for Unconventional Transmissible Agents, Paris, France.|APHP, Pitié-Salpêtrière Hospital, Paris, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Haïk
authors.affiliation: Institute of Neurological Sciences, Scientific Institute for Research, Hospitalization and Health Care, Bologna, Italy.|Department of Biomedical and Neuromotor Sciences, University of Bologna, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Parchi
authors.affiliation: Department of Neurological Sciences, National Institute of Health, Rome, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pocchiari
authors.affiliation: National CJD Research & Surveillance Unit, Western General Hospital, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
authors.forename: Alison
authors.initials: A
authors.lastname: Green
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D005260:Female; D006801:Humans; D038622:Internationality; D008297:Male; D008875:Middle Aged; D011328:Prions / Q000737:chemistry; D066263:Protein Aggregation, Pathological / Q000134:cerebrospinal fluid*; D011994:Recombinant Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011328:Prions; D011994:Recombinant Proteins