title: Benefits of Selenium Supplementation on Leukocyte DNA Integrity Interact with Dietary Micronutrients: A Short Communication.
abstract: A male cohort from New Zealand has previously shown variability in Selenium (Se) supplementation effects on measured biomarkers. The current analysis is to understand the reasons for variability of the H₂O₂-induced DNA damage recorded after Se supplementation. We have looked at the variation of demographic, lifestyle, medication, genetic and dietary factors and biomarkers measured at baseline and post-supplementation in these two extreme subgroups A and B. Group A showed increased H₂O₂-induced DNA damage and group B showed decreased damage after Se supplementation. We have also considered correlations of biomarkers and dietary factors in the complete dataset. The glutathione peroxidase (GPx) activity and DNA damage were significantly lower at post-supplementation in Group B compared to Group A. Post-supplementation, Group B showed a significant reduction in the GPx activity, while Group A showed a significant increase in DNA damage compared to baseline levels. Dietary methionine intake was significantly higher and folate intake was significantly lower in Group B compared to Group A. Se supplementation significantly increased the caspase-cleaved keratin 18 levels in both groups, indicating increased apoptotic potential of this supplement. Parameter correlation with the complete dataset showed dietary methionine to have a significant negative correlation with H₂O₂-induced DNA damage post-supplementation. The data suggest that Se supplementation is beneficial for the leukocyte DNA integrity only in interaction with the dietary methionine and folate intake.
authors.affiliation: Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. n.karunasinghe@auckland.ac.nz.
authors.forename: Nishi
authors.initials: N
authors.lastname: Karunasinghe
authors.affiliation: Discipline of Nutrition, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. st.zhu@auckland.ac.nz.
authors.forename: Shuotun
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. l.ferguson@auckland.ac.nz.|Discipline of Nutrition, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. l.ferguson@auckland.ac.nz.
authors.forename: Lynnette R
authors.initials: LR
authors.lastname: Ferguson
mesh_terms: D000328:Adult; D000368:Aged; D015331:Cohort Studies; D004249:DNA Damage / Q000187:drug effects*; D015930:Diet Records; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D018977:Micronutrients; D008875:Middle Aged; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
chemical_list: D018977:Micronutrients; D051140:Selenoproteins; D005979:Glutathione Peroxidase; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
keywords: DNA damage; GPx activity; Se supplementation; caspase-cleaved keratin 18; dietary folate; dietary methionine