title: A colorimetric and fluorescent probe for detecting intracellular biothiols.
abstract: A new rapid and highly sensitive coumarin-based probe (probe 1) has been designed and synthesized for detecting intracellular thiols. Probe 1 was prepared by a 4-step procedure as a latent fluorescence probe to achieve high sensitivity and fluorescence turn-on response toward cysteine and homocysteine over GSH and other various natural amino acids under physiological conditions. Owing to specific cyclization between thiols and aldehyde group, probe 1 displayed a highly selectivity toward cysteine and homocysteine. Above all, probe 1 was successfully used for fluorescence imaging of biothiols in Hela cells, and quantitative determination had been achieved within a certain range. Then specific fluorescence imaging of mice organ tissues was obtained for proving the permeability of probe 1. Simultaneously, the viability was measured to be more than 80%, which shows probe 1 can be a rapid and biocompatible probe for biothiols in cells. Furthermore, the measurement of thiols detection in 5 kinds of animal serum showed that probe 1 can be used in determination of biothiols in blood.
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address: liuws@lzu.edu.cn.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D003124:Colorimetry / Q000379:methods*; D003374:Coumarins / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis* / Q000097:blood; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis* / Q000097:blood; D006041:Goats; D006367:HeLa Cells; D006710:Homocysteine / Q000032:analysis* / Q000097:blood; D006736:Horses; D006801:Humans; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D012756:Sheep; D013050:Spectrometry, Fluorescence / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D006710:Homocysteine; C030123:coumarin; D005978:Glutathione; D003545:Cysteine
keywords: Bioimaging; Colorimetric; Coumarin; Fluorescent; Probe; Thiols