title: One-Step Cartilage Repair Technique as a Next Generation of Cell Therapy for Cartilage Defects: Biological Characteristics, Preclinical Application, Surgical Techniques, and Clinical Developments.
abstract: To provide a comprehensive overview of the basic science rationale, surgical technique, and clinical outcomes of 1-step cartilage repair technique used as a treatment strategy for cartilage defects.
  
  
  
  A systematic review was performed in the main medical databases to evaluate the several studies concerning 1-step procedures for cartilage repair. The characteristics of cell-seed scaffolds, behavior of cells seeded into scaffolds, and surgical techniques were also discussed. Clinical outcomes and quality of repaired tissue were assessed using several standardized outcome assessment tools, magnetic resonance imaging scans, and biopsy histology.
  
  
  
  One-step cartilage repair could be divided into 2 types: chondrocyte-matrix complex (CMC) and autologous matrix-induced chondrogenesis (AMIC), both of which allow a simplified surgical approach. Studies with Level IV evidence have shown that 1-step cartilage repair techniques could significantly relieve symptoms and improve functional assessment (P < .05, compared with preoperative evaluation) at short-term follow-up. Furthermore, magnetic resonance imaging showed that 76% cases in all included case series showed at least 75% defect coverage in each lesion, and 3 studies clearly showed hyaline-like cartilage tissue in biopsy tissues by second-look arthroscopy.
  
  
  
  The 1-step cartilage repair technique, with its potential for effective, homogeneous distribution of chondrocytes and multipotent stem cells on the surface of the cartilage defect, is able to regenerate hyaline-like cartilage tissue, and it could be applied to cartilage repair by arthroscopy.
  
  
  
  Level IV, systematic review of Level II and IV studies.
authors.affiliation: Department of Orthopedics and the Center for Sport Medicine, the First Affiliated Hospital, College of Medicine Zhejiang University, Hangzhou, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics and the Center for Sport Medicine, the First Affiliated Hospital, College of Medicine Zhejiang University, Hangzhou, China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine School of Medicine, Zhejiang University, Hangzhou, China; Zhejiang Xingyue Biotechnology Company, Hangzhou, China.
authors.forename: You-Zhi
authors.initials: YZ
authors.lastname: Cai
authors.affiliation: Department of Orthopedics and the Center for Sport Medicine, the First Affiliated Hospital, College of Medicine Zhejiang University, Hangzhou, China. Electronic address: locuszc@icloud.com.
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Lin
mesh_terms: D001182:Arthroscopy; D002358:Cartilage, Articular / Q000293:injuries / Q000601:surgery*; D019902:Chondrocytes / Q000637:transplantation; D020219:Chondrogenesis; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D039902:Multipotent Stem Cells / Q000637:transplantation; D054457:Tissue Scaffolds