title: The Chemopreventive Peptide Lunasin Inhibits d-Galactose- Induced Experimental Cataract in Rats.
abstract: Oxidative damage to the constituents of the eye lens is a major mechanism in the initiation and development of cataract. Lunasin, a 43-amino acids chemoprevention peptide, has been proved to possess potent anti-oxidative activity other than its established anticancer activities. Herein, we explored whether lunasin has preventative effects on d-galactose-induced experimental cataract in rat. After modeling, SD rats were administrated by instillation, 80 µM of lunasin eye drops to each eye thrice daily and consecutively for 30 days. As a result, lunasin treatment effectively inhibited the progression of d-galactose-induced experimental cataract, and protected the lenses of rats from oxidative damage and attenuated the lipid peroxidation through up-regulation of antioxidant enzymes, and inhibited the activation of polyol pathway by decreasing AR activity. Additionally, in vitro studies proved that lunasin treatment could protect human lens epithelial cells (hLECs) against d-galactose induced cell damage and apoptosis, and up-regulate antioxidant enzymes. This is the first demonstration that lunasin could inhibit d-galactose-induced experimental cataract in rats by protecting against oxidative damage and inhibiting the activation of polyol pathway.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Dai
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Pei
authors.initials: P
authors.lastname: Ye
authors.forename: Miaoqing
authors.initials: M
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.forename: Haoyue
authors.initials: H
authors.lastname: Shuai
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University 211# mailbox, 24 Tongjia Street, Nanjing 210009, People`s Republic of China. tohike@hotmail.com.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Tan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002386:Cataract / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002460:Cell Line; D005260:Female; D005690:Galactose; D006801:Humans; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: C120514:GM2S-1 protein, soybean; D020011:Protective Agents; D030262:Soybean Proteins; D005690:Galactose