title: [Overexpression of IL-8 promotes migration of BT549 breast cancer cells].
abstract: OBJECTIVE
  To construct a recombinant adenovirus vector containing IL-8 gene and observe its effect on the proliferation, cell cycle and migration of BT549 breast cancer cells.
  
  
  METHODS
  IL-8 gene was amplified by PCR using the cDNA from 143B bone sarcoma cells and inserted into shuttle plasmid pAdTrack-TO4. The recombinant shuttle plasmid pAdTrack-TO4-IL-8 was digested by PmeI and then transformed to AdEasier competent cells. The obtained recombinant adenovirus plasmid pAdIL-8 was digested by PacI, and then transfected to HEK293 cells for package and amplification by Lipofectamine(TM) 2000. The titer was tested by dilution assay. The expression of IL-8 mRNA and protein in BT549 cells was detected by reverse transcription PCR and ELISA, respectively. Effect of IL-8 overexpression on proliferation, cell cycle and migration in BT549 cells was respectively investigated by MTT assay, flow cytometry and wound-healing test.
  
  
  RESULTS
  PCR and DNA sequence analysis verified the recombinant shuttle plasmid pAdTrack-TO4-IL-8. Restriction enzymes PacI confirmed the recombinant adenovirus plasmid pAdIL-8. IL-8 was overexpressed in BT549 cells after AdIL-8 infection. Overexpression of IL-8 promoted BT549 cell migration and arrested the cell cycle in the S phase, but it made no significant difference in the proliferation of BT549 cells.
  
  
  CONCLUSIONS
  IL-8 overexpression can promote migration of BT549 breast cancer cells.
authors.affiliation: Ministry of Education Key Laboratory for Laboratory Medical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Deng
authors.affiliation: Ministry of Education Key Laboratory for Laboratory Medical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory for Laboratory Medical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Mengtian
authors.initials: M
authors.lastname: Fan
authors.affiliation: Ministry of Education Key Laboratory for Laboratory Medical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yangliu
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Ministry of Education Key Laboratory for Laboratory Medical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory for Laboratory Medical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. *Corresponding author, E-mail:anniesq8718@aliyun.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D001943:Breast Neoplasms / Q000473:pathology*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*
chemical_list: D016209:Interleukin-8