title: A photothermally responsive nanoprobe for bioimaging based on Edman degradation.
abstract: A new type of photothermally responsive nanoprobe based on Edman degradation has been synthesized and characterized. Under irradiation by an 808 nm laser, the heat generated by the gold nanorod core breaks the thiocarbamide structure and releases the fluorescent dye Cy5.5 with increased near-infrared (NIR) fluorescence under mild acidic conditions. This RGD modified nanoprobe is capable of fluorescence imaging of ανβ3 over-expressing U87MG cells in vitro and in vivo. This Edman degradation-based nanoprobe provides a novel strategy to design activatable probes for biomedical imaging and drug/gene delivery.
authors.affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China. maqj@jlu.edu.cn and Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Zhantong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Lixin
authors.initials: L
authors.lastname: Lang
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Qianjun
authors.initials: Q
authors.lastname: He
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Nan
authors.initials: N
authors.lastname: Lu
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Yijing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Jibin
authors.initials: J
authors.lastname: Song
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China. maqj@jlu.edu.cn.
authors.forename: Shi
authors.initials: S
authors.lastname: Gao
authors.affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China. maqj@jlu.edu.cn.
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Dale O
authors.initials: DO
authors.lastname: Kiesewetter
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA. dkiesewetter@mail.nih.gov shawn.chen@nih.gov.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes; D018014:Gene Transfer Techniques; D006046:Gold; D006801:Humans; D051379:Mice; D043942:Nanotubes; D009369:Neoplasms / Q000000981:diagnostic imaging*; D020349:Surface Plasmon Resonance
chemical_list: D005456:Fluorescent Dyes; D006046:Gold