title: Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.-Hill.
abstract: Background. Duguetia furfuracea is popular plant used in popular medicine. Hypothesis/Purpose. This claim evaluated the phytochemical composition of the hydroethanolic extract (HEDF), fractions of Duguetia furfuracea, and antioxidant and antifungal activity. Methods. The chemical profile was carried out by HPLC-DAD. The total phenolic contents and flavonoid components were determined by Folin-Ciocalteu and aluminium chloride reaction. The antioxidant activity was measured by scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and ferric reducing ability of plasma (FRAP) methods. The antifungal activity was determined by microdilution assay.
  
  
  RESULTS
  HPLC analysis revealed caffeic acid and rutin as major compounds (HEDF), caffeic acid and quercitrin (Mt-OH fraction), and quercitrin and isoquercitrin (Ac-OEt fraction). The highest levels of phenols and total flavonoids were found for Ac-OEt fraction, and the crude extract showed higher in vitro antioxidant potential. The antifungal activity showed synergic effect with fluconazole and EHDF against C. krusei, fluconazole and Mt-OH against C. krusei and C. tropicalis, and Ac-OE and fluconazole against C. albicans. Conclusion. The highest levels of phenols and total flavonoids were marked with antioxidant effect. This is the first report of bioactivity of the synergic effect of HEDF and fractions. More studies would be required to better clarify its mechanism of synergic action.
authors.affiliation: Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry (LFQM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil; Department of Biochemistry and Molecular Biology, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.
authors.forename: Francisca Valéria Soares de Araújo
authors.initials: FV
authors.lastname: Pinho
authors.affiliation: Department of Biochemistry and Molecular Biology, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.
authors.forename: Litiele Cezar
authors.initials: LC
authors.lastname: da Cruz
authors.affiliation: Interdisciplinary Center for Biotechnology Research (CIPBIOTEC), Federal University of Pampa (UNIPAMPA), 97300-000 Sao Gabriel, RS, Brazil.
authors.forename: Nathane Rosa
authors.initials: NR
authors.lastname: Rodrigues
authors.affiliation: Department of Biochemistry and Molecular Biology, Postgraduate Program in Toxicological Biochemistry, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.
authors.forename: Emily Pansera
authors.initials: EP
authors.lastname: Waczuk
authors.affiliation: Department of Biological Chemistry, Laboratory of Microbiology and Molecular Chemistry (LMBM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil.
authors.forename: Celestina Elba Sobral
authors.initials: CE
authors.lastname: Souza
authors.affiliation: Department of Biological Chemistry, Laboratory of Microbiology and Molecular Chemistry (LMBM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil.
authors.forename: Henrique Douglas Melo
authors.identifier: 0000-0002-6634-4207
authors.initials: HD
authors.lastname: Coutinho
authors.affiliation: Department of Biological Chemistry, Laboratory of Studies on Natural Products (LPPN), Regional University of Cariri (URCA), 63105-000 Crato, CE, Brazil.
authors.forename: José Galberto Martins
authors.initials: JG
authors.lastname: da Costa
authors.affiliation: Department of Industrial Pharmacy, Phytochemical Research Laboratory, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.affiliation: Department of Industrial Pharmacy, Phytochemical Research Laboratory, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil.
authors.forename: Aline Augusti
authors.identifier: 0000-0002-6001-1313
authors.initials: AA
authors.lastname: Boligon
authors.affiliation: Interdisciplinary Center for Biotechnology Research (CIPBIOTEC), Federal University of Pampa (UNIPAMPA), 97300-000 Sao Gabriel, RS, Brazil.
authors.forename: Jeferson Luis
authors.identifier: 0000-0002-3874-8618
authors.initials: JL
authors.lastname: Franco
authors.affiliation: Interdisciplinary Center for Biotechnology Research (CIPBIOTEC), Federal University of Pampa (UNIPAMPA), 97300-000 Sao Gabriel, RS, Brazil.
authors.forename: Thaís
authors.identifier: 0000-0002-6491-5854
authors.initials: T
authors.lastname: Posser
authors.affiliation: Department of Biological Chemistry, Laboratory of Pharmacology and Molecular Chemistry (LFQM), Regional University of Cariri (URCA), 63100-000 Crato, CE, Brazil.
authors.forename: Irwin Rose Alencar
authors.initials: IR
authors.lastname: de Menezes
mesh_terms: D029061:Annonaceae / Q000737:chemistry*; D000935:Antifungal Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D005658:Fungi / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000494:pharmacology; D014867:Water / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts; D014867:Water; D000431:Ethanol