title: A High-Adhesive Lysine-Cyclic RGD Peptide Designed for Selective Cell Retention Technology.
abstract: Cell adhesion is an important property of biomaterials used in selective cell retention (SCR) technology, which fabricates bone grafts rapidly in clinical settings. This could be improved by physical and biologic manipulations. To facilitate retention of the cells on the scaffold, especially osteoprogenitors from bone marrow in the convenient SCR procedure, a lysine-cyclic RGD (LcRGD) peptide was here designed to coordinate positively charged amino acids and the RGD sequence to enhance the adhesion performance of the scaffold. Demineralized bone matrix (DBM) is an important therapeutic resource, but its cell adhesion ability and osteoinductive capacity are low because of its processing. These capabilities can be increased to enhance the performance of DBM when used in SCR technology. Here, LcRGD peptide was used to modify DBM and produce a DBM/LcRGD composite. This composite exhibited enhanced adhesion performance on cultured human bone marrow-derived mesenchymal stem cells and retained more osteoprogenitors from bone marrow than other materials did. The DBM/LcRGD composite displayed a preferable osteoinduction in vitro and osteogenic capacity in vivo. Thus, LcRGD peptide as a commendable modifier of DBM applied in SCR technology can improve bone transplantation.
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Keyu
authors.initials: K
authors.lastname: Luo
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Tieniu
authors.initials: T
authors.lastname: Mei
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Moyuan
authors.initials: M
authors.lastname: Deng
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Zehua
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Tianyong
authors.initials: T
authors.lastname: Hou
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|4 Department of Biomedical Materials Science, College of Biomedical Engineering, The Third Military Medical University , Chongqing, China .
authors.forename: Shiwu
authors.initials: S
authors.lastname: Dong
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xie
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Xu
authors.affiliation: 1 Department of Orthopedics, Southwest Hospital, National & Regional United Engineering Laboratory of Tissue Engineering, The Third Military Medical University , Chongqing, China .|2 Center of Regenerative and Reconstructive Engineering Technology in Chongqing City , Chongqing, China .|3 Tissue Engineering Laboratory of Chongqing City , Chongqing, China .
authors.forename: Fei
authors.initials: F
authors.lastname: Luo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D007085:Ilium / Q000166:cytology* / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D008239:Lysine / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D010012:Osteogenesis / Q000502:physiology*; D010456:Peptides, Cyclic / Q000737:chemistry*; D033581:Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D016023:Integrins; D010456:Peptides, Cyclic; C507169:cyclic arginine-glycine-aspartic acid peptide; D008239:Lysine