title: An Evaluation of Blood Compatibility of Silver Nanoparticles.
abstract: Silver nanoparticles (AgNPs) have tremendous potentials in medical devices due to their excellent antimicrobial properties. Blood compatibility should be investigated for AgNPs due to the potential blood contact. However, so far, most studies are not systematic and have not provided insights into the mechanisms for blood compatibility of AgNPs. In this study, we have investigated the blood biological effects, including hemolysis, lymphocyte proliferation, platelet aggregation, coagulation and complement activation, of 20 nm AgNPs with two different surface coatings (polyvinyl pyrrolidone and citrate). Our results have revealed AgNPs could elicit hemolysis and severely impact the proliferation and viability of lymphocytes at all investigated concentrations (10, 20, 40 μg/mL). Nevertheless, AgNPs didn't show any effect on platelet aggregation, coagulation process, or complement activation at up to ~40 μg/mL. Proteomic analysis on AgNPs plasma proteins corona has revealed that acidic and small molecular weight blood plasma proteins were preferentially adsorbed onto AgNPs, and these include some important proteins relevant to hemostasis, coagulation, platelet, complement activation and immune responses. The predicted biological effects of AgNPs by proteomic analysis are mostly consistent with our experimental data since there were few C3 components on AgNPs and more negative than positive factors involving platelet aggregation and thrombosis.
authors.affiliation: Key Laboratory of Standardization and Measurement for Nanotechnology of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Lai
authors.affiliation: Key Laboratory of Standardization and Measurement for Nanotechnology of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Menghua
authors.initials: M
authors.lastname: Cui
authors.affiliation: Key Laboratory of Standardization and Measurement for Nanotechnology of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.|College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, P. R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Liang
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Standardization and Measurement for Nanotechnology of Chinese Academy of Sciences, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
authors.forename: Liming
authors.initials: L
authors.lastname: Xie
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002951:Citrates / Q000737:chemistry / Q000494:pharmacology; D003167:Complement Activation / Q000187:drug effects; D019295:Computational Biology / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010974:Platelet Aggregation / Q000187:drug effects; D011205:Povidone / Q000737:chemistry / Q000494:pharmacology; D061251:Primary Cell Culture; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D012834:Silver / Q000494:pharmacology*; D000077559:Sodium Citrate
chemical_list: D000890:Anti-Infective Agents; D001798:Blood Proteins; D002951:Citrates; D020543:Proteome; D000077559:Sodium Citrate; D012834:Silver; D011205:Povidone