title: Usage of DNA Fingerprinting Technology for Quality Control in Molecular Lab Bench Work.
abstract: One of the major quality assurance (QA) goals in many molecular laboratories is to avoid sample pipetting errors on the lab bench; especially when pipetting into multiwell plates. A pipetting error can cause a switch in patient samples, which can lead to recording the wrong results for the patient samples involved. Such pipetting errors are difficult to identify when it happens in lab bench work. DNA fingerprinting is a powerful tool in determining sample identities. Our laboratory has explored the usage of this technology in our QA process and successfully established that DNA fingerprinting can be used to monitor possible sample switch in gene rearrangement lab bench work. We use florescent light to quench the florescence in the gene rearrangement polymerase chain reaction products. After that, DNA fingerprinting technology is used to identify the sample DNA in the gene rearrangement polymerase chain reaction plate. The result is compared with the corresponding patient's blood sample DNA to determine whether there is a sample switch during the lab bench work.
authors.affiliation: Molecular Diagnostic Laboratory, H. Lee Moffitt Cancer Center, Tampa, FL.
authors.forename: Linda Y
authors.initials: LY
authors.lastname: McIntosh
authors.forename: Janella E
authors.initials: JE
authors.lastname: Lal
authors.forename: Dahui
authors.initials: D
authors.lastname: Qin
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods* / Q000592:standards*; D016172:DNA Fingerprinting; D006801:Humans; D016133:Polymerase Chain Reaction; D011786:Quality Control; D000073886:Scientific Experimental Error