title: TM4SF1 Promotes Proliferation, Invasion, and Metastasis in Human Liver Cancer Cells.
abstract: Transmembrane 4 superfamily member 1 (TM4SF1) is a member of tetraspanin family, which mediates signal transduction events regulating cell development, activation, growth and motility. Our previous studies showed that TM4SF1 is highly expressed in liver cancer. HepG2 cells were transfected with TM4SFl siRNA and TM4SF1-expressing plasmids and their biological functions were analyzed in vitro and in vivo. HepG2 cells overexpressing TM4SF1 showed reduced apoptosis and increased cell migration in vitro and enhanced tumor growth and metastasis in vivo, whereas siRNA-mediated silencing of TM4SF1 had the opposite effect. TM4SF1 exerts its effect by regulating a few apoptosis- and migration-related genes including caspase-3, caspase-9, MMP-2, MMP-9 and VEGF. These results indicate that TM4SF1 is associated with liver tumor growth and progression, suggesting that TM4SF1 may be a potential target for treatment of liver cancer in future.
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China. hyukun@hotmail.com.|Key Laboratory of Viral Hepatitis, Central South University, Changsha 410008, China. hyukun@hotmail.com.
authors.forename: Yu-Kun
authors.initials: YK
authors.lastname: Huang
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China. dian1044@126.com.|Key Laboratory of Viral Hepatitis, Central South University, Changsha 410008, China. dian1044@126.com.
authors.forename: Xue-Gong
authors.initials: XG
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Xiangya Third Hospital, Central South University, Changsha 410013, China. qiuffu@hotmail.com.
authors.forename: Fu
authors.initials: F
authors.lastname: Qiu
mesh_terms: D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D056945:Hep G2 Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D009363:Neoplasm Proteins; D042461:Vascular Endothelial Growth Factor A; C074075:TM4SF1 protein, human; D053148:Caspase 3; D053453:Caspase 9; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: TM4SF1; liver cancer; metastasis; proliferation