title: Lack of Buffering by Composites Promotes Shift to More Cariogenic Bacteria.
abstract: Secondary caries (SC) remains a very important problem with composite restorations. The objectives of this study were to test the acid-buffering ability of several restorative materials and to evaluate whether buffering of the restorative material has an impact on the microbial composition of the biofilm. Disk-shaped specimens of conventional composite, composite with surface prereacted glass-ionomer filler particles (so-called giomer), glass-ionomer cement (GIC), amalgam, and hydroxyapatite (HAp) (control) were exposed to aqueous solutions with pH 4, 5, 6, and 7 and to the medium containing bacteria-produced acids, and pH changes were recorded over several days. Next, material specimens were immersed in bacterial growth medium with pH adjusted to 5. After a 24-h incubation, the extracts were collected and inoculated with a cariogenic (Streptococcus mutans) and a noncariogenic (Streptococcus sanguinis) species. The bacterial growth was monitored both in a single-species model by spectrophotometry and in a dual-species model by viability quantitative polymerase chain reaction. Amalgam and HAp showed the strongest acid-buffering ability, followed by the GIC and the giomer, while the conventional composite did not exhibit any buffering capacity. Furthermore, due to the lack of acid-buffering abilities, composite was not able to increase the pH of the medium (pH 5), which, in the absence of antibacterial properties, allowed the growth of S. mutans, while the growth of S. sanguinis, a less aciduric species, was completely inhibited. A similar effect was observed when bacteria were cultured together: there was a higher percentage of S. mutans and lower percentage of S. sanguinis with the conventional composite than with other materials and HAp. In conclusion, conventional composites lack the ability to increase the local pH, which leads to the outgrowth of more acidogenic/aciduric bacteria and higher cariogenicity of the biofilm. Together with lack of antibacterial properties, lack of buffering may account for the higher susceptibility of composites to SC.
authors.affiliation: KU Leuven BIOMAT, Department of Oral Health Sciences, University of Leuven & Dentistry University Hospitals Leuven, Leuven, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Nedeljkovic
authors.affiliation: KU Leuven BIOMAT, Department of Oral Health Sciences, University of Leuven & Dentistry University Hospitals Leuven, Leuven, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: De Munck
authors.affiliation: Oral Microbiology, Department of Oral Health Sciences, University of Leuven & Dentistry University Hospitals Leuven, Leuven, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Slomka
authors.affiliation: KU Leuven BIOMAT, Department of Oral Health Sciences, University of Leuven & Dentistry University Hospitals Leuven, Leuven, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Van Meerbeek
authors.affiliation: Oral Microbiology, Department of Oral Health Sciences, University of Leuven & Dentistry University Hospitals Leuven, Leuven, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Teughels
authors.affiliation: KU Leuven BIOMAT, Department of Oral Health Sciences, University of Leuven & Dentistry University Hospitals Leuven, Leuven, Belgium kirsten.vanlanduyt@med.kuleuven.be.
authors.forename: K L
authors.initials: KL
authors.lastname: Van Landuyt
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D002021:Buffers; D003188:Composite Resins / Q000009:adverse effects / Q000627:therapeutic use*; D003723:Dental Amalgam / Q000009:adverse effects / Q000627:therapeutic use; D003733:Dental Caries Susceptibility / Q000187:drug effects*; D003793:Dental Restoration, Permanent / Q000009:adverse effects; D017886:Durapatite / Q000009:adverse effects / Q000627:therapeutic use; D005899:Glass Ionomer Cements / Q000009:adverse effects / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D013291:Streptococcus / Q000187:drug effects* / Q000254:growth & development; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development
chemical_list: D002021:Buffers; D003188:Composite Resins; D005899:Glass Ionomer Cements; D003723:Dental Amalgam; D017886:Durapatite
keywords: Streptococcus mutans; Streptococcus sanguinis; amalgam; dental plaque; glass-ionomer cement; quantitative real-time PCR