title: Single cell migration dynamics mediated by geometric confinement.
abstract: The migration dynamics of cells plays a key role in tissue engineering and regenerative medicine. Previous studies mostly focus on regulating stem cell fate and phenotype by biophysical cues. In contrast, less is known about how the geometric cues mediate the migration dynamics of cells. Here, we fabricate graphene oxide (GO) microstripes on cell non-adhesive PEG substrate by using micromolding in capillary (MIMIC) method. Such micropatterns with alternating cell adhesion and cell resistance enable an effective control of selective adhesion and migration of single cells. The sharp contrast in cell adhesion minimizes the invasion of cells into the PEG patterns, and thereby strongly confines the cells on GO microstripes. As a result, the cells are forced to adapt highly polarized, elongated, and oriented geometry to fit the patterns. A series of pattern widths have been fabricated to modulate the extent of cell deformation and polarization. Under strong confinement, the cytoskeleton contractility, intracellular traction, and actin filament elongation are highly promoted, which result in enhanced cell migration along the patterns. This work provides an important insight into developing combinatorial graphene-based patterns for the control of cell migration dynamics, which is of great significance for tissue engineering and regenerative medicine.
authors.affiliation: Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science, Ningbo 315201, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science, Ningbo 315201, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Hou
authors.affiliation: Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science, Ningbo 315201, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Rubo
authors.initials: R
authors.lastname: Xing
authors.affiliation: Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science, Ningbo 315201, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Han
authors.affiliation: Institute of Printing and Packaging Engineering, Xi'an University of Technology, Xi'an 710048, China.
authors.forename: Penggang
authors.initials: P
authors.lastname: Ren
authors.affiliation: Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Science, Ningbo 315201, China. Electronic address: fujun@nimte.ac.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
mesh_terms: D002465:Cell Movement / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010087:Oxides / Q000737:chemistry*
chemical_list: D010087:Oxides; D006108:Graphite
keywords: Cell migration; Cytoskeleton contractility; Graphene oxide micropatterns; Osteoblasts