title: Chromosome identification for the carnivorous plant Genlisea margaretae.
abstract: Genlisea margaretae, subgenus Genlisea, section Recurvatae (184 Mbp/1C), belongs to a plant genus with a 25-fold genome size difference and an extreme genome plasticity. Its 19 chromosome pairs could be distinguished individually by an approach combining optimized probe pooling and consecutive rounds of multicolor fluorescence in situ hybridization (mcFISH) with bacterial artificial chromosomes (BACs) selected for repeat-free inserts. Fifty-one BACs were assigned to 18 chromosome pairs. They provide a tool for future assignment of genomic sequence contigs to distinct chromosomes as well as for identification of homeologous chromosome regions in other species of the carnivorous Lentibulariaceae family, and potentially of chromosome rearrangements, in cases where more than one BAC per chromosome pair was identified.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Stadt Seeland, Germany.|Plant Resource Center, Vietnam Academy of Agricultural Science, Ankhanh, Hoaiduc, Hanoi, Vietnam.
authors.forename: Trung D
authors.initials: TD
authors.lastname: Tran
authors.affiliation: Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany, CZ-78371, Olomouc, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Šimková
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Stadt Seeland, Germany.
authors.forename: Renate
authors.initials: R
authors.lastname: Schmidt
authors.affiliation: Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany, CZ-78371, Olomouc, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Stadt Seeland, Germany.|Central European Institute of Technology and Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Ingo
authors.initials: I
authors.lastname: Schubert
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Stadt Seeland, Germany. fuchs@ipk-gatersleben.de.
authors.forename: Jörg
authors.initials: J
authors.lastname: Fuchs
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics* / Q000378:metabolism; D059646:Genome Size; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D019684:Magnoliopsida / Q000235:genetics / Q000378:metabolism
keywords: BACs; Genlisea; Karyotyping; Multicolor fluorescence in situ hybridization (mcFISH); Reprobing