title: Optimization of pineapple pulp residue hydrolysis for lipid production by Rhodotorula glutinis TISTR5159 using as biodiesel feedstock.
abstract: The higher lipid productivity of Rhodotorula glutinis TISTR5159 was achieved by optimizing the pineapple pulp hydrolysis for releasing the high sugars content. The sequential simplex method operated by varied; solid-to-liquid ratio, sulfuric acid concentration, temperature, and hydrolysis time were successfully applied and the highest sugar content (83.2 g/L) evaluated at a solid-to-liquid ratio of 1:10.8, 3.2% sulfuric acid, 105 °C for 13.9 min. Moreover, the (NH4)2SO4 supplement enhanced the lipid productivity and gave the maximum yields of biomass and lipid of 15.2 g/L and 9.15 g/L (60.2%), respectively. The C16 and C18 fatty acids were found as main components included oleic acid (55.8%), palmitic acid (16.6%), linoleic acid (11.9%), and stearic acid (7.8%). These results present the possibility to convert the sugars in pineapple pulp hydrolysate to lipids. The fatty acid profile was also similar to vegetable oils. Thus, it could be used as potential feedstock for biodiesel production.
authors.affiliation: a Department of Chemistry, Faculty of Science , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Jidapha
authors.initials: J
authors.lastname: Tinoi
authors.affiliation: a Department of Chemistry, Faculty of Science , Chiang Mai University , Chiang Mai , Thailand.|b Faculty of Health Science , Nation University , Lampang , Thailand.
authors.forename: Nuansri
authors.initials: N
authors.lastname: Rakariyatham
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D031230:Ananas / Q000737:chemistry / Q000378:metabolism*; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D005227:Fatty Acids / Q000096:biosynthesis*; D005285:Fermentation; D005638:Fruit / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D050155:Lipogenesis; D012248:Rhodotorula / Q000378:metabolism*; D013464:Sulfuric Acids / Q000737:chemistry; D013696:Temperature
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D013464:Sulfuric Acids; C033158:sulfuric acid; D000645:Ammonium Sulfate
keywords: Rhodotorula glutinis; biodiesel feedstock; lipid production; pineapple pulp; sequential simplex optimization