title: A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry.
abstract: In this study, a novel method combining dispersive liquid-liquid microextraction (DLLME) and fast liquid chromatography coupled to mass spectrometry (LC-MS/MS) was developed and validated for the extraction and determination of bisphenol A (BPA) and six bisphenol analogues, namely bisphenol S (BPS), bisphenol F (BPF), bisphenol P (BPP), bisphenol Z (BPZ), bisphenol AP (BPAP) and bisphenol AF (BPAF) in human urine samples. Type and volume of extraction and disperser solvents, pH sample, ionic strength, and agitation were evaluated. The matrix-matched calibration curves of all analytes were linear with correlation coefficients higher than 0.99 in the range level of 0.5-20.0ngmL(-1). The relative standard deviation (RSD), precision, at three concentrations (1.0, 8.0 and 15.0ngmL(-1)) was lower than 15% with accuracy ranging from 90 to 112%. The biomonitoring capability of the new method was confirmed with the analysis of 50 human urine samples randomly collected from Brazilians. BPA was detected in 92% of the analyzed samples at concentrations ranging <LOQ to 10.4ngmL(-1). The detection rates of BPS (10%), BPAF (4%) and BPF (2%) were much lower than 92%.
authors.affiliation: Laboratório de Toxicologia e Essencialidade de Metais, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14049-903 Ribeirão Preto, SP, Brazil.
authors.forename: Bruno Alves
authors.initials: BA
authors.lastname: Rocha
authors.affiliation: Laboratório de Toxicologia e Essencialidade de Metais, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14049-903 Ribeirão Preto, SP, Brazil.
authors.forename: Bruno Ruiz Brandão
authors.initials: BR
authors.lastname: da Costa
authors.affiliation: Departmento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14049-901 Ribeirão Preto, SP, Brazil.
authors.forename: Nayara Cristina Perez
authors.initials: NC
authors.lastname: de Albuquerque
authors.affiliation: Departmento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14049-901 Ribeirão Preto, SP, Brazil.
authors.forename: Anderson Rodrigo Moraes
authors.initials: AR
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Toxicologia e Essencialidade de Metais, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14049-903 Ribeirão Preto, SP, Brazil.
authors.forename: Juliana Maria Oliveira
authors.initials: JM
authors.lastname: Souza
authors.affiliation: Department of Chemistry, University of Central Florida, Orlando, FL, USA.
authors.forename: Maha
authors.initials: M
authors.lastname: Al-Tameemi
authors.affiliation: Department of Chemistry, University of Central Florida, Orlando, FL, USA.
authors.forename: Andres Dobal
authors.initials: AD
authors.lastname: Campiglia
authors.affiliation: Laboratório de Toxicologia e Essencialidade de Metais, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14049-903 Ribeirão Preto, SP, Brazil. Electronic address: fbarbosa@fcfrp.usp.br.
authors.forename: Fernando
authors.initials: F
authors.lastname: Barbosa
mesh_terms: D001559:Benzhydryl Compounds; D001938:Brazil; D002853:Chromatography, Liquid; D006801:Humans; D059627:Liquid Phase Microextraction; D010636:Phenols; D053719:Tandem Mass Spectrometry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Biomonitoring; Bisphenol A; Dispersive liquid-liquid microextraction; Fast method; Liquid chromatography-tandem mass spectrometry; Urine