title: TERRA promotes telomerase-mediated telomere elongation in Schizosaccharomyces pombe.
abstract: Telomerase-mediated telomere elongation provides cell populations with the ability to proliferate indefinitely. Telomerase is capable of recognizing and extending the shortest telomeres in cells; nevertheless, how this mechanism is executed remains unclear. Here, we show that, in the fission yeast Schizosaccharomyces pombe, shortened telomeres are highly transcribed into the evolutionarily conserved long noncoding RNA TERRA A fraction of TERRA produced upon telomere shortening is polyadenylated and largely devoid of telomeric repeats, and furthermore, telomerase physically interacts with this polyadenylated TERRA in vivo We also show that experimentally enhanced transcription of a manipulated telomere promotes its association with telomerase and concomitant elongation. Our data represent the first direct evidence that TERRA stimulates telomerase recruitment and activity at chromosome ends in an organism with human-like telomeres.
authors.affiliation: Institute of Biochemistry (IBC), Eidgenössische Technische Hochschule Zürich (ETHZ), Zürich, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Moravec
authors.affiliation: Institute of Biochemistry (IBC), Eidgenössische Technische Hochschule Zürich (ETHZ), Zürich, Switzerland.
authors.forename: Harry
authors.initials: H
authors.lastname: Wischnewski
authors.affiliation: Institute of Biochemistry (IBC), Eidgenössische Technische Hochschule Zürich (ETHZ), Zürich, Switzerland.
authors.forename: Amadou
authors.initials: A
authors.lastname: Bah
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Institute of Biochemistry (IBC), Eidgenössische Technische Hochschule Zürich (ETHZ), Zürich, Switzerland.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lafranchi
authors.affiliation: Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
authors.forename: Megan C
authors.initials: MC
authors.lastname: King
authors.affiliation: Institute of Biochemistry (IBC), Eidgenössische Technische Hochschule Zürich (ETHZ), Zürich, Switzerland claus.azzalin@bc.biol.ethz.ch.
authors.forename: Claus M
authors.identifier: 0000-0002-9396-1980
authors.initials: CM
authors.lastname: Azzalin
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D011061:Poly A; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics* / Q000378:metabolism*; D059505:Telomere Homeostasis; D059506:Telomere Shortening; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D011061:Poly A; D019098:Telomerase
keywords: TERRA; fission yeast; telomerase; telomere length regulation; transcription