title: A biotransformation process for the production of cucurbitacin B from its glycoside using a selected Streptomyces sp.
abstract: Cucurbitacin B (CuB) and its glycoside, cucurbitacin B 2-o-β-D-glucoside (CuBg), abundantly occur in the pedicels of Cucumis melo. Compared with CuB, CuBg is not efficiently extracted from the pedicels. Furthermore, the anticancer activity of CuBg is lower than that of the aglycone. A process for CuBg biotransformation to CuB was developed for the first time. A strain of Streptomyces species that converts CuBg into CuB was isolated from an enrichment culture of C. melo pedicels. After optimization of conditions for enzyme production and biotransformation, a maximum conversion rate of 92.6 % was obtained at a CuBg concentration of 0.25 g/L. When biotransformation was performed on C. melo pedicel extracts, the CuB concentration in the extracts increased from 1.50 to 3.27 g/L. The conversion rate was almost 100 %. The developed process may be an effective biotransformation method for industrial production CuB from C. melo pedicels for pharmaceuticals.
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Xiacheng District, Hangzhou, 310032, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Mei
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Xiacheng District, Hangzhou, 310032, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Xiacheng District, Hangzhou, 310032, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Jin
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Xiacheng District, Hangzhou, 310032, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Tang
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Xiacheng District, Hangzhou, 310032, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yi
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Xiacheng District, Hangzhou, 310032, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, 18 Chaowang Road, Xiacheng District, Hangzhou, 310032, China. bioph@zjut.edu.cn.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Ying
mesh_terms: D001711:Biotransformation; D006027:Glycosides / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D006027:Glycosides; D014315:Triterpenes; C041246:cucurbitacin B
keywords: Biotransformation; Cucurbitacin B; Cucurbitacin B 2-o-β-D-glucoside; Streptomyces sp.