title: Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device.
abstract: Efficient separations of particles with micron and submicron dimensions are extremely useful in preparation and analysis of materials for nanotechnological and biological applications. Here, we demonstrate a nonintuitive, yet efficient, separation mechanism for μm and subμm colloidal particles and organelles, taking advantage of particle transport in a nonlinear post array in a microfluidic device under the periodic action of electrokinetic and dielectrophoretic forces. We reveal regimes in which deterministic particle migration opposite to the average applied force occurs for a larger particle, a typical signature of deterministic absolute negative mobility (dANM), whereas normal response is obtained for smaller particles. The coexistence of dANM and normal migration was characterized and optimized in numerical modeling and subsequently implemented in a microfluidic device demonstrating at least 2 orders of magnitude higher migration speeds as compared to previous ANM systems. We also induce dANM for mouse liver mitochondria and envision that the separation mechanisms described here provide size selectivity required in future separations of organelles, nanoparticles, and protein nanocrystals.
authors.affiliation: School of Molecular Sciences and ⊥Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.|Department of Biochemistry, Molecular Biology and Biophysics, and §Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Luo
authors.affiliation: School of Molecular Sciences and ⊥Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.|Department of Biochemistry, Molecular Biology and Biophysics, and §Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Muratore
authors.affiliation: School of Molecular Sciences and ⊥Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.|Department of Biochemistry, Molecular Biology and Biophysics, and §Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Edgar A
authors.initials: EA
authors.lastname: Arriaga
authors.affiliation: School of Molecular Sciences and ⊥Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.|Department of Biochemistry, Molecular Biology and Biophysics, and §Department of Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Ros
mesh_terms: D000818:Animals; D051379:Mice; D046210:Microfluidic Analytical Techniques; D008930:Mitochondria, Liver / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D015388:Organelles / Q000737:chemistry*; D010316:Particle Size; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011506:Proteins