title: Inflammation-related alterations of lipids after spinal cord injury revealed by Raman spectroscopy.
abstract: Spinal cord injury (SCI) triggers several lipid alterations in nervous tissue. It is characterized by extensive demyelination and the inflammatory response leads to accumulation of activated microglia/macrophages, which often transform into foam cells by accumulation of lipid droplets after engulfment of the damaged myelin sheaths. Using an experimental rat model, Raman microspectroscopy was applied to retrieve the modifications of the lipid distribution following SCI. Coherent anti-Stokes Raman scattering (CARS) and endogenous two-photon fluorescence (TPEF) microscopies were used for the detection of lipid-laden inflammatory cells. The Raman mapping of CH2 deformation mode intensity at 1440  cm(−1) retrieved the lipid-depleted injury core. Preserved white matter and inflammatory regions with myelin fragmentation and foam cells were localized by specifically addressing the distribution of esterified lipids, i.e., by mapping the intensity of the carbonyl Raman band at 1743  cm(−1), and were in agreement with CARS/TPEF microscopy. Principal component analysis revealed that the inflammatory regions are notably rich in saturated fatty acids. Therefore, Raman spectroscopy enabled to specifically detect inflammation after SCI and myelin degradation products.
authors.affiliation: TU Dresden, Klinisches Sensoring und Monitoring-Klinik für Anästhesiologie und Intensivtherapie, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Tamosaityte
authors.affiliation: TU Dresden, Klinisches Sensoring und Monitoring-Klinik für Anästhesiologie und Intensivtherapie, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Roberta
authors.initials: R
authors.lastname: Galli
authors.affiliation: TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Ortrud
authors.initials: O
authors.lastname: Uckermann
authors.affiliation: TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Kerim H
authors.initials: KH
authors.lastname: Sitoci-Ficici
authors.affiliation: TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, GermanycCRTD/DFG-Center for Regenerative Therapies Dresden-Cluster of Excellence, Fetscherstr. 105, 01307 Dresden, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Koch
authors.affiliation: TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Later
authors.affiliation: TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schackert
authors.affiliation: TU Dresden, Klinisches Sensoring und Monitoring-Klinik für Anästhesiologie und Intensivtherapie, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Edmund
authors.initials: E
authors.lastname: Koch
authors.affiliation: TU Dresden, Klinisches Sensoring und Monitoring-Klinik für Anästhesiologie und Intensivtherapie, Fetscherstr. 74, 01307 Dresden, Germany.
authors.forename: Gerald
authors.initials: G
authors.lastname: Steiner
authors.affiliation: TU Dresden, Neurochirurgie, Universitätsklinikum Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, GermanycCRTD/DFG-Center for Regenerative Therapies Dresden-Cluster of Excellence, Fetscherstr. 105, 01307 Dresden, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kirsch
mesh_terms: D000818:Animals; D007249:Inflammation / Q000000981:diagnostic imaging; D008055:Lipids / Q000737:chemistry*; D009186:Myelin Sheath / Q000473:pathology; D051381:Rats; D013059:Spectrum Analysis, Raman; D013119:Spinal Cord Injuries / Q000000981:diagnostic imaging*
chemical_list: D008055:Lipids