title: In Vivo Ablation of a Dendritic Cell Subset Expressing the Chemokine Receptor XCR1.
abstract: Dendritic cells (DCs) are one of the key populations controlling immune responses. To establish a cell depletion system in vivo, human diphtheria toxin (DT) receptor (DTR) is transduced to the mice in which DTR is expressed under the control of a specific promoter. In these mice, DTR-expressing cells are inducibly depleted after DT injection. Using this system, analysis of mouse models in which DTR was expressed under the CD11c promoter has contributed to our knowledge of DC biology by depleting CD11c(+) cells. Other mouse models to inducibly eliminate specific DC subsets upon DT treatment have been also generated. Here, we describe a new mouse model in which the XCR1(+) DC subset is inducibly and transiently depleted in vivo.
authors.affiliation: Laboratory for Immune Regulation, WPI Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.|Laboratory of Inflammatory Regulation, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.|Department of Immunology, Institute of Advanced Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama, Wakayama, 641-8509, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hemmi
authors.affiliation: Laboratory for Immune Regulation, WPI Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.|Laboratory of Inflammatory Regulation, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.|Department of Immunology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan.
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Hoshino
authors.affiliation: Laboratory for Immune Regulation, WPI Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan. tkaisho@wakayama-med.ac.jp.|Laboratory of Inflammatory Regulation, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. tkaisho@wakayama-med.ac.jp.|Department of Immunology, Institute of Advanced Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama, Wakayama, 641-8509, Japan. tkaisho@wakayama-med.ac.jp.
authors.forename: Tsuneyasu
authors.initials: T
authors.lastname: Kaisho
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000235:genetics*; D003713:Dendritic Cells / Q000276:immunology*; D004167:Diphtheria Toxin / Q000494:pharmacology*; D055879:Gene Knock-In Techniques; D066257:Heparin-binding EGF-like Growth Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D011401:Promoter Regions, Genetic; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*
chemical_list: D039521:CD11c Antigen; D004167:Diphtheria Toxin; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D019707:Receptors, Chemokine; C551408:XC chemokine receptor 1, mouse
keywords: Dendritic cell subset; Dendritic cells; Diphtheria toxin; Diphtheria toxin receptor; Inducible ablation of dendritic cell subsets