title: Isolation and biological characterization of tendon-derived stem cells from fetal bovine.
abstract: The lack of appropriate candidates of cell sources for cell transplantation has hampered efforts to develop therapies for tendon injuries, such as tendon rupture, tendonitis, and tendinopathy. Tendon-derived stem cells (TDSCs) are a type of stem cells which may be used in the treatment of tendon injuries. In this study, TDSCs were isolated from 5-mo-old Luxi Yellow fetal bovine and cultured in vitro and further analyzed for their biological characteristics using immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR) assays. It was found that primary TDSCs could be expanded for 42 passages in vitro maintaining proliferation. The expressions of stem cell marker nucleostemin and tenocyte-related markers, such as collagen I, collagen II, collagen III, and tenascin-C, were observed on different passage cells by immunofluorescence. The results from RT-PCR show that TDSCs were positive for collagen type I, CD44, tenascin-C, and collagen type III but negative for collagen type II. Meanwhile, TDSC passage 4 was successfully induced to differentiate into osteoblasts, adipocytes, and chondrocytes. Our results indicate that the fetal bovine TDSCs not only had strong self-renewal capacity but also possess the potential for multi-lineage differentiation. This study provides theoretical basis and experimental foundation for potential therapeutic application of the fetal bovine TDSCs in the treatment of tendon injuries.
authors.affiliation: Institute of Physical Education, University of Jimei, No. 185, Yinjiang Road, Jimei District, 361021, Xiamen City, Fujian Province, People's Republic of China.|Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People's Republic of China.
authors.forename: Jinjuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People's Republic of China.
authors.forename: Qianjun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People's Republic of China.
authors.forename: Kunfu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Animal Genetic Resources (AnGR), Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People's Republic of China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Ma
authors.affiliation: Institute of Physical Education, University of Jimei, No. 185, Yinjiang Road, Jimei District, 361021, Xiamen City, Fujian Province, People's Republic of China. huanghongmei@jmu.edu.cn.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Physical Education, University of Jimei, No. 185, Yinjiang Road, Jimei District, 361021, Xiamen City, Fujian Province, People's Republic of China. yangxiaonuodexiao@163.com.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D019902:Chondrocytes / Q000166:cytology; D005333:Fetus / Q000166:cytology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D013708:Tendon Injuries / Q000473:pathology / Q000628:therapy*; D013710:Tendons / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Fetal bovine; Genes; Immunofluorescence; Markers; Multi-differentiation; RT-PCR; Tendon-derived stem cells (TDSCs); Therapeutic application