title: Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade.
abstract: Specification of the myriad of unique neuronal subtypes found in the nervous system depends upon spatiotemporal cues and terminal selector gene cascades, often acting in sequential combinatorial codes to determine final cell fate. However, a specific neuronal cell subtype can often be generated in different parts of the nervous system and at different stages, indicating that different spatiotemporal cues can converge on the same terminal selectors to thereby generate a similar cell fate. However, the regulatory mechanisms underlying such convergence are poorly understood. The Nplp1 neuropeptide neurons in the Drosophila ventral nerve cord can be subdivided into the thoracic-ventral Tv1 neurons and the dorsal-medial dAp neurons. The activation of Nplp1 in Tv1 and dAp neurons depends upon the same terminal selector cascade: col>ap/eya>dimm>Nplp1. However, Tv1 and dAp neurons are generated by different neural progenitors (neuroblasts) with different spatiotemporal appearance. Here, we find that the same terminal selector cascade is triggered by Kr/pdm>grn in dAp neurons, but by Antp/hth/exd/lbe/cas in Tv1 neurons. Hence, two different spatiotemporal combinations can funnel into a common downstream terminal selector cascade to determine a highly related cell fate.
authors.affiliation: Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors.forename: Hugo
authors.initials: H
authors.lastname: Gabilondo
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Johannes
authors.initials: J
authors.lastname: Stratmann
authors.affiliation: Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Rubio-Ferrera
authors.affiliation: Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Millán-Crespo
authors.affiliation: Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Contero-García
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Bahrampour
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Stefan
authors.identifier: 0000-0002-7602-5322
authors.initials: S
authors.lastname: Thor
authors.affiliation: Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors.forename: Jonathan
authors.identifier: 0000-0002-1159-3644
authors.initials: J
authors.lastname: Benito-Sipos
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D050980:GATA Transcription Factors / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D050809:POU Domain Factors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D050980:GATA Transcription Factors; D018398:Homeodomain Proteins; C497244:Kr protein, Drosophila; D051741:Kruppel-Like Transcription Factors; D060850:LIM-Homeodomain Proteins; C086967:Lbe protein, Drosophila; D009479:Neuropeptides; C518480:Nplp1 protein, Drosophila; D050809:POU Domain Factors; D014157:Transcription Factors; C074459:ap protein, Drosophila; C096148:grn protein, Drosophila; C104357:kn protein, Drosophila; C104121:nub protein, Drosophila