title: Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012.
abstract: Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities.
  
  
  
  A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected.
  
  
  
  Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases.
  
  
  
  Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.
authors.affiliation: Duke-NUS Medical School, 8 College Rd, Singapore.
authors.forename: Celeste
authors.initials: C
authors.lastname: Donato
authors.affiliation: National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam.
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Hoi
authors.affiliation: Department of Clinical Microbiology, Hanoi Medical University, Vietnam.
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Hoa
authors.affiliation: National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam.
authors.forename: Tran Mai
authors.initials: TM
authors.lastname: Hoa
authors.affiliation: National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam.
authors.forename: Le
authors.initials: L
authors.lastname: Van Duyet
authors.affiliation: National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam.
authors.forename: Ta Thi
authors.initials: TT
authors.lastname: Dieu Ngan
authors.affiliation: National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam.
authors.forename: Nguyen
authors.initials: N
authors.lastname: Van Kinh
authors.affiliation: National Hospital of Tropical Diseases, 78 Giai Phong Street, Hanoi, Vietnam.
authors.forename: Nguyen
authors.initials: N
authors.lastname: Vu Trung
authors.affiliation: Duke-NUS Medical School, 8 College Rd, Singapore; Department of Pathology, Singapore General Hospital, Singapore. Electronic address: vijay.dhanasekaran@duke-nus.edu.sg.
authors.forename: Dhanasekaran
authors.initials: D
authors.lastname: Vijaykrishna
mesh_terms: D017677:Age Distribution; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D029821:Enterovirus A, Human / Q000145:classification* / Q000235:genetics*; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000266:history / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D062306:Geography, Medical; D006232:Hand, Foot and Mouth Disease / Q000453:epidemiology / Q000821:virology; D049674:History, 21st Century; D006760:Hospitalization; D006801:Humans; D007223:Infant; D010802:Phylogeny; D012621:Seasons; D014744:Vietnam / Q000453:epidemiology
chemical_list: D036022:Capsid Proteins
keywords: C4 subgenogroup; Enterovirus 71; Outbreak; Phylogenetics; Vietnam