title: [TLR9 expression is positively correlated with the levels of CD38, HLA-DR and CD95 on peripheral blood mononuclear cells in chronic HBV infected patients].
abstract: OBJECTIVE
  To explore the relationship between the expression of TLR9 and the levels of CD38, HLA-DR and CD95 on peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B virus (HBV) infected patients.
  
  
  METHODS
  70 chronic HBV infected patients and 12 healthy donors were enrolled in this study, and density gradient centrifugation was used to isolate PBMCs from peripheral blood with EDTA for anticoagulation. Flow cytometry was used to detect the levels of TLR9, CD38, HLA-DR and CD95 on PBMCs.
  
  
  RESULTS
  Compared to the healthy donors, chronic HBV infected patients with low viral load or high viral load had significantly higher levels of TLR9, HLA-DR and CD95 on PMBCs. Furthermore, the co-expression rates of TLR9 and CD38, HLA-DR, CD95 on PBMCs were obviously higher than those of the healthy donors. Correlation analysis showed that the expression of TLR9 was positively correlated with CD38 (r=0.345), HLA-DR (r=0.334), CD95 (r=0.227) on PBMCs in the patients with chronic HBV infection.
  
  
  CONCLUSIONS
  The expression of TLR9 increased and was positively associated with CD38, HLA-DR and CD95 on PBMCs during chronic HBV infection.
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Laboratory Medicine, Guangxi Yulin Blood Center, Yulin 537000, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Mao
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Lishan
authors.initials: L
authors.lastname: Peng
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Liu
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Dengrong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China. *Corresponding authors, E-mail: xiaojian6666@hotmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China. *Corresponding authors, E-mail: lj986771558@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Leng
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000097:blood*; D000328:Adult; D005260:Female; D006684:HLA-DR Antigens / Q000097:blood*; D019694:Hepatitis B, Chronic / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood*; D008875:Middle Aged; D051217:Toll-Like Receptor 9 / Q000097:blood*; D019014:fas Receptor / Q000097:blood*
chemical_list: C491901:FAS protein, human; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D019014:fas Receptor; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1