title: Quantitative proteomic analysis of normal and degenerated human intervertebral disc.
abstract: Degenerative disc disease (DDD) is the most common disease of aging in humans. DDD is characterized by the gradual damage of the intervertebral discs. The disease is characterized by progressive dehydration of nucleus pulposus and disruption of annulus fibrosus of intervertebral disc.
  
  
  
  Even though it is highly prevalent, there is no effective therapy to regenerate the degenerated disc, or decrease or halt the disease progression. Therefore, novel monitoring and diagnostic tests are essential to develop an alternative therapeutic strategies which can prevent further progression of disc degeneration.
  
  
  
  The study was designed to understand the proteome map of annulus fibrosus and nucleus pulposus tissues of intervertebral disc and its differential expression in patients with DDD.
  
  
  
  The proteome map of the annulus fibrosus and nucleus pulposus tissues of intervertebral disc was cataloged involving one-dimensional gel electrophoresis-Fourier transform mass spectrometry/ion trap tandem mass spectrometry (FTMS/ITMSMS) analysis. The altered proteome patterns of annulus fibrosus and nucleus pulposus tissues for DDD were identified using Isobaric tag for relative and absolute quantification (iTRAQ)-based quantitative proteomics coupled with FTMS/ITMSMS and network pathway analysis.
  
  
  
  The study identified a total of 759 and 692 proteins from the annulus fibrosus and the nucleus pulposus tissues of the disc based on FTMS/ITMSMS analysis, which includes 118 proteins commonly identified between the two tissues. Vibrant changes were observed between the normal and the degenerating annulus fibrosus and nucleus pulposus tissues. A total of 73 and 54 proteins were identified as differentially regulated in the annulus and the nucleus tissues, respectively, between the normal and the degenerated tissues independently. Network pathway analysis mapped the differentially expressed proteins to cell adhesion, cell migration, and interleukin13 signaling pathways.
  
  
  
  Altogether, the current study provides a novel vision in the biomechanism of human disc degeneration and a certain number of proteins with the potential biomarker value for the preliminary diagnosis and scenario of DDD.
authors.affiliation: E114, CSIR-CCMB, Uppal Rd, Hyderabad, 500007, India.
authors.forename: Nukala
authors.initials: N
authors.lastname: Sarath Babu
authors.affiliation: Sunshine Hospital, Penderghast Rd, Secunderabad, 500003, India.
authors.forename: Sivaraman
authors.initials: S
authors.lastname: Krishnan
authors.affiliation: Proteomics Facility, CSIR-Centre for Cellular and Molecular Biology, Uppal Rd, Hyderabad, 500007, India.
authors.forename: Cherukuvada V
authors.initials: CV
authors.lastname: Brahmendra Swamy
authors.affiliation: Sunshine Hospitals, SMART (Sunshine Medical Academy For Research and Training), Penderghast Rd, Secunderabad, 500003, India.
authors.forename: Goli P
authors.initials: GP
authors.lastname: Venkata Subbaiah
authors.affiliation: Sunshine Hospital, Penderghast Rd, Secunderabad, 500003, India.
authors.forename: Annapareddy V
authors.initials: AV
authors.lastname: Gurava Reddy
authors.affiliation: W001, CSIR-CCMB, Uppal Rd, Hyderabad, 500007, India. Electronic address: idris@ccmb.res.in.
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: Idris
mesh_terms: D016022:Case-Control Studies; D006801:Humans; D007403:Intervertebral Disc / Q000378:metabolism*; D055959:Intervertebral Disc Degeneration / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020543:Proteome
keywords: Annulus fibrosus; Degenerative disc disease; Human spine; Intervertebral disc degeneration; Network pathway; Nucleus pulposus; Proteome mapping; Quantitative proteomics