title: Curcumin-docetaxel co-loaded nanosuspension for enhanced anti-breast cancer activity.
abstract: A curcumin-docetaxel co-loaded nanosuspension with increased anti-breast cancer activity was developed. Curcumin is a potential anticancer agent with p-glycoprotein (p-gp) inhibiting activity may be co-administered with docetaxel as a nanosuspension to enhance its anticancer effect by increasing the oral bioavailability and decreasing drug efflux.
  
  
  
  Nanosuspensions of curcumin and docetaxel were prepared by precipitation-homozenisation technique and evaluated for particle size, polydispersity, zeta potential and drug release. The in vitro MTT assay was conducted using MCF-7 for anti-breast cancer activity. The in vivo biodistribution by radiolabeling and tumor inhibition study was conducted in mice.
  
  
  
  Homogenous nanosuspensions of 80 ± 20 nm were obtained with increased solubility. The drugs as nanosuspensions showed higher cytotoxicity on MCF-7 cell line compared to their suspensions due to the increased in vitro cellular uptake. Due to this increased solubility, sensitization of tumor cells and inhibition of p-gp the in-vivo results showed greater tumor inhibition rate of up to 70% in MCF-7 treated mice. Histopathological results showed higher apoptotic activity and reduced level of angiogenesis.
  
  
  
  The in vitro and in vivo study of the nanosuspensions has shown that Co-administration of Curcumin as a p-gp inhibitor with docetaxel may have the potential to increase the anti-breast cancer efficacy of both drugs.
authors.affiliation: a Department of Pharmaceutics , GIPS, Guwahati University , Guwahati , India.
authors.forename: Bhanu P
authors.initials: BP
authors.lastname: Sahu
authors.affiliation: a Department of Pharmaceutics , GIPS, Guwahati University , Guwahati , India.
authors.forename: Hemanga
authors.initials: H
authors.lastname: Hazarika
authors.affiliation: b Department of Bioengineering & Technology , Guwahati University , Guwahati , India.
authors.forename: Rituraj
authors.initials: R
authors.lastname: Bharadwaj
authors.affiliation: c Bioscience & Bioengineering , Indian Institute of Technology (IIT) , Guwahati , India.
authors.forename: Pojul
authors.initials: P
authors.lastname: Loying
authors.affiliation: d Nuclear Medicine Division , Indian Institute of Chemical Biology (IICB) , Kolkata , India.
authors.forename: Rinku
authors.initials: R
authors.lastname: Baishya
authors.affiliation: a Department of Pharmaceutics , GIPS, Guwahati University , Guwahati , India.
authors.forename: Suvakanta
authors.initials: S
authors.lastname: Dash
authors.affiliation: e Department of Pharmaceutical Sciences , Dibrugarh University , Dibrugarh , India.
authors.forename: Malay K
authors.initials: MK
authors.lastname: Das
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000818:Animals; D001682:Biological Availability; D001943:Breast Neoplasms / Q000188:drug therapy*; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D000077143:Docetaxel; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D053758:Nanoparticles; D010316:Particle Size; D012995:Solubility; D013535:Suspensions; D043823:Taxoids / Q000008:administration & dosage / Q000494:pharmacology*; D014018:Tissue Distribution
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D013535:Suspensions; D043823:Taxoids; D000077143:Docetaxel; D003474:Curcumin
keywords: Curcumin; MCF-7; biodistribution; breast cancer; docetaxel; nanaosuspension; radiolabeling