title: Therapeutic effect of ozone and rutin on adriamycin-induced testicular toxicity in an experimental rat model.
abstract: To evaluate the cytoprotective effects of rutin, ozone and their combination on adriamycin (ADR)-induced testicular toxicity, 50 male albino rats were classified into five groups of ten animals each as follows: placebo group; ADR group; ADR + rutin group; ADR + ozone group and ADR + rutin + ozone group. Sperm functions, testosterone (T), luteinising hormone (LH), follicle stimulating hormone (FSH), testicular enzymes, oxidant/antioxidant status, C-reactive protein, monocyte chemoattractant proteins-1 and leukotriene B4 were determined. After ADR injection, a decline in sperm functions was observed. FSH and LH levels were increased, T level and testicular enzymes were decreased, significant enhancement in oxidative stress with subsequent depletion in antioxidants was detected and inflammatory markers were significantly elevated. Treatment with rutin and/or ozone, however, improved the aforementioned parameters. Ozone therapy alone almost completely reversed the toxic effects of ADR and restored all parameters to normal levels.
authors.affiliation: Department of Urology, Zagazig University, Zagazig, Sharkia, Egypt.
authors.forename: E A
authors.initials: EA
authors.lastname: Salem
authors.affiliation: Medical Division, Department of Narcotics, Ergogenics and Poisons, National Research Centre, Cairo, Egypt.
authors.forename: N A
authors.initials: NA
authors.lastname: Salem
authors.affiliation: Department of Urology, Tulane University, New Orleans, LA, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Hellstrom
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D004317:Doxorubicin / Q000633:toxicity*; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D020011:Protective Agents; D013739:Testosterone; D012431:Rutin; D010126:Ozone; D004317:Doxorubicin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Adriamycin; oxidative stress; ozone; rutin; testicular toxicity