title: Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skin.
abstract: The improvement of dendritic cell subset isolation from tissues and the use of appropriate surface markers allowed to decipher their heterogeneity but also allowed to unravel some specific functions that are valuable for vaccine design as well as for a better understanding of the in situ pathophysiology upon infection. Here, we describe the procedures to extract those cells from the skin and to analyze them by flow cytometry using a combination of appropriate surface markers allowing further transcriptomic analysis and functional assays.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), UM2 Aix-Marseille Université, Avenue de Luminy, 13288, Marseille Cedex 9, France.|INSERM U1104, 13288, Marseille Cedex 9, France.|CNRS UMR7280, 13288, Marseille Cedex 9, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Malosse
authors.affiliation: Centre d'Immunologie de Marseille-Luminy (CIML), UM2 Aix-Marseille Université, Avenue de Luminy, 13288, Marseille Cedex 9, France. henri@ciml.univ-mrs.fr.|INSERM U1104, 13288, Marseille Cedex 9, France. henri@ciml.univ-mrs.fr.|CNRS UMR7280, 13288, Marseille Cedex 9, France. henri@ciml.univ-mrs.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Henri
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D051379:Mice; D012867:Skin / Q000166:cytology* / Q000276:immunology
chemical_list: D015415:Biomarkers
keywords: Collagenase 4; Dermis; Dispase; Epidermis; Fc Block; Flow cytometry; Monoclonal antibodies; Percoll; Skin