title: Transcriptomics Analysis of Candida albicans Treated with Huanglian Jiedu Decoction Using RNA-seq.
abstract: Candida albicans is the major invasive fungal pathogen of humans, causing diseases ranging from superficial mucosal infections to disseminated, systemic infections that are often life-threatening. Resistance of C. albicans to antifungal agents and limited antifungal agents has potentially serious implications for management of infections. As a famous multiherb prescription in China, Huanglian Jiedu Decoction (HLJJD, Orengedokuto in Japan) is efficient against Trichophyton mentagrophytes and C. albicans. But the antifungal mechanism of HLJDD remains unclear. In this study, by using RNA-seq technique, we performed a transcriptomics analysis of gene expression changes for C. albicans under the treatment of HLJDD. A total of 6057 predicted protein-encoding genes were identified. By gene expression analysis, we obtained a total of 735 differentially expressed genes (DEGs), including 700 upregulated genes and 35 downregulated genes. Genes encoding multidrug transporters such as ABC transporter and MFS transporter were identified to be significantly upregulated. Meanwhile, by pathway enrichment analysis, we identified 26 significant pathways, in which pathways of DNA replication and transporter activity were mainly involved. These results might provide insights for the inhibition mechanism of HLJDD against C. albicans.
authors.affiliation: Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou 310006, China; College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Shandong Provincial Research Center for Bioinformatic Engineering and Technique, School of Life Sciences, Shandong University of Technology, 266 West Cunxi Road, Zibo 255049, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou 310006, China.
authors.forename: Maocan
authors.initials: M
authors.lastname: Tao
authors.affiliation: Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou 310006, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou 310006, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou 310006, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao