title: Oxidative stress response of the aquatic macrophyte Hydrilla verticillata exposed to TiO2nanoparticles.
abstract: The present study investigated the effects of titanium dioxide nanoparticles (TiO2-NPs) on the oxidative stress response in Hydrilla verticillata. Macrophytes were exposed to different concentrations of TiO2-NPs (0 mg/L, 0.01 mg/L, 0.1 mg/L, 1 mg/L, and 10 mg/L) for 24 h, based on currently predicted levels of nano-TiO2in surface waters. In addition, TiO2-NPs with varying crystalline status were used to assess the potential influence of crystalline phases on oxidative stress responses. The level of hydrogen peroxide (H2O2), reduced and oxidized glutathione (GSH and GSSG), and activities of the antioxidative enzymes peroxidase (POD), catalase (CAT), and glutathione reductase (GR) were measured and compared with a bulk counterpart. Although POD was not considered to be active, the results imply an activation of the enzymatic defense system, because increased CAT and GR activities were observed. Exposure to bulk TiO2revealed lower enzyme activities at all exposure concentrations, suggesting a nano-specific influence on the antioxidative defense mechanisms in H. verticillata. Moreover, all TiO2-NP concentrations resulted in a decreased GSH/GSSG ratio, indicating high GSH-dependent metabolic activity to protect against the destructive effects of reactive oxygen species (ROS) generated during nano-TiO2exposure. As the level of H2O2was solely elevated after exposure to 10 mg/L of P25, it appears plausible that the adaptive metabolic mechanisms of H. verticillata are able to cope with environmentally relevant concentrations of TiO2-NPs. Environ Toxicol Chem 2016;35:2859-2866. © 2016 SETAC.
authors.affiliation: Ecological Impact Research and Ecotoxicology, Technische Universität Berlin, Berlin, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Okupnik
authors.affiliation: Ecological Impact Research and Ecotoxicology, Technische Universität Berlin, Berlin, Germany. stephan.pflugmacher@tu-berlin.de.
authors.forename: Stephan
authors.initials: S
authors.lastname: Pflugmacher
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019803:Glutathione Disulfide / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D031321:Hydrocharitaceae / Q000187:drug effects* / Q000378:metabolism; D053758:Nanoparticles / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014025:Titanium / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; C009495:titanium dioxide; D014025:Titanium; D002374:Catalase; D009195:Peroxidase; D005980:Glutathione Reductase; D019803:Glutathione Disulfide
keywords: Aquatic plants; Nanoparticles; Oxidative stress; Stress response; Titanium dioxide nanoparticles