title: A ratiometric fluorescent probe based on boron dipyrromethene and rhodamine Förster resonance energy transfer platform for hypochlorous acid and its application in living cells.
abstract: We have developed a ratiometric fluorescent probe BRT based on boron dipyrromethene (BODIPY) and rhodamine-thiohydrazide Förster resonance energy transfer (FRET) platform for sensing hypochlorous acid (HOCl) with high selectivity and sensitivity. The probe can detect HOCl in 15 s with the detection limit of 38 nM. Upon mixing with HOCl the fluorescence colour of probe BRT changed from green to orange. Moreover, probe BRT was applied to successfully monitor HOCl in living RAW 264.7 cells.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Zhao
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China. Electronic address: miaojy@sdu.edu.cn.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006997:Hypochlorous Acid / Q000032:analysis*; D057230:Limit of Detection; D051379:Mice; D061848:Optical Imaging / Q000379:methods; D011162:Porphobilinogen / Q000031:analogs & derivatives* / Q000737:chemistry; D000067996:RAW 264.7 Cells; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001896:Boron Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; C507544:dipyrromethene; D006997:Hypochlorous Acid; D011162:Porphobilinogen
keywords: Boron dipyrromethene; Fluorescence; Hypochlorous acid; Living cells; Ratiometric; Rhodamine-thiohydrazide