title: Overlap-Based Cell Tracker.
abstract: In order to facilitate the extraction of quantitative data from live cell image sets, automated image analysis methods are needed. This paper presents an introduction to the general principle of an overlap cell tracking software developed by the National Institute of Standards and Technology (NIST). This cell tracker has the ability to track cells across a set of time lapse images acquired at high rates based on the amount of overlap between cellular regions in consecutive frames. It is designed to be highly flexible, requires little user parameterization, and has a fast execution time.
authors.affiliation: Software and Systems Division.
authors.forename: Joe
authors.initials: J
authors.lastname: Chalfoun
authors.affiliation: Software and Systems Division.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cardone
authors.affiliation: Software and Systems Division.
authors.forename: Alden A
authors.initials: AA
authors.lastname: Dima
authors.affiliation: Software and Systems Division.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Allen
authors.affiliation: Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Halter
keywords: cell motility; live-cell imaging; overlap-based cell tracking; time-lapse cell imaging