title: Antiulcer and antiproliferative properties of spent brewer's yeast peptide extracts for incorporation into foods.
abstract: The main objective was to study the antiulcer and antiproliferative potential of yeast peptide extract for further incorporation into functional foods. Peptide concentrates were obtained by hydrolysis of spent brewer's yeast proteins followed by a filtration process. In order to prove the possible protection of gastric mucosa, an animal model with ulcerative lesions caused by oral administration of absolute ethanol was used. The peptide fraction <3 kDa was able to reduce gastric injuries to significant levels (p < 0.001) and the effective dose (DE50) was 816 mg per kg bw. The cytoprotective effect appears to depend on a prostaglandin-mediated mechanism and also on a nonspecific mechanism. The antiproliferative activity of the extract in nine different human tumoral cell lines was tested. The results exhibited a promising antiproliferative activity against the cell line K-562 (leukemia). The results suggest that a new peptide extract can be used to develop new value-added functional food products, although further studies are required.
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital Apartado 2511, 4202-401 Porto, Portugal. mpintado@porto.ucp.pt.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Amorim
authors.forename: Joana O
authors.initials: JO
authors.lastname: Pereira
authors.forename: Karin M
authors.initials: KM
authors.lastname: Monteiro
authors.forename: Ana L
authors.initials: AL
authors.lastname: Ruiz
authors.forename: João E
authors.initials: JE
authors.lastname: Carvalho
authors.forename: Hélder
authors.initials: H
authors.lastname: Pinheiro
authors.forename: Manuela
authors.initials: M
authors.lastname: Pintado
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002522:Chlorocebus aethiops; D000431:Ethanol / Q000009:adverse effects; D055951:Functional Food; D005656:Fungal Proteins / Q000008:administration & dosage / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D019073:HT29 Cells; D006801:Humans; D006868:Hydrolysis; D007938:Leukemia; D061986:MCF-7 Cells; D008297:Male; D023421:Models, Animal; D009569:Nitric Oxide / Q000494:pharmacology; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D011453:Prostaglandins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D014709:Vero Cells
chemical_list: D000897:Anti-Ulcer Agents; D005656:Fungal Proteins; D010455:Peptides; D011453:Prostaglandins; D009569:Nitric Oxide; D000431:Ethanol