title: Secretome Profiling of Periodontal Ligament from Deciduous and Permanent Teeth Reveals a Distinct Expression Pattern of Laminin Chains.
abstract: It has been suggested that there are histological and functional distinctions between the periodontal ligament (PDL) of deciduous (DecPDL) and permanent (PermPDL) teeth. Thus, we hypothesized that DecPDL and PermPDL display differences in the constitutive expression of genes/proteins involved with PDL homeostasis. Primary PDL cell cultures were obtained for DecPDL (n = 3) and PermPDL (n = 3) to allow us to perform label-free quantitative secretome analysis. Although a highly similar profile was found between DecPDL and PermPDL cells, comparative secretome analysis evidenced that one of the most stickling differences involved cell adhesion molecules, including laminin subunit gamma 1 (LAMC1) and beta 2 (LAMB2). Next, total RNA and protein extracts were obtained from fresh PDL tissues of deciduous (n = 6) and permanent (n = 6) teeth, and Western blotting and qPCR analysis were used to validate our in vitro findings. Western blot analysis confirmed that LAMC1 was increased in DecPDL fresh tissues (p<0.05). Furthermore, qPCR data analysis revealed that mRNA levels for laminin subunit beta 1 (LAMB1), beta 3 (LAMB3), LAMC1, and gamma 2 (LAMC2) were higher in DecPDL fresh tissues, whereas transcripts for LAMB2 were increased in PermPDL (p<0.05). In conclusion, the differential expression of laminin chains in DecPDL and PermPDL suggests an involvement of laminin-dependent pathways in the control of physiological differences between them.
authors.affiliation: Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
authors.forename: Priscila A
authors.initials: PA
authors.lastname: Giovani
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Salmon
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
authors.forename: Luciane
authors.initials: L
authors.lastname: Martins
authors.affiliation: Brazilian Biosciences National Laboratory, LNBio, CNPEM, Campinas, São Paulo, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
authors.affiliation: Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
authors.forename: Pedro
authors.initials: P
authors.lastname: Rebouças
authors.affiliation: Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
authors.forename: Regina M
authors.initials: RM
authors.lastname: Puppin Rontani
authors.affiliation: Department of Genetics, Institute of Biology, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Luciana S
authors.initials: LS
authors.lastname: Mofatto
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
authors.forename: Enilson A
authors.initials: EA
authors.lastname: Sallum
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.
authors.forename: Francisco H
authors.initials: FH
authors.lastname: Nociti
authors.affiliation: Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, São Paulo, Brazil.|Department of Dental Materials, São Leopoldo Mandic Research Center, Campinas, São Paulo, Brazil.
authors.forename: Kamila R
authors.initials: KR
authors.lastname: Kantovitz
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D019229:Dentition, Permanent; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008297:Male; D010513:Periodontal Ligament / Q000378:metabolism*; D014094:Tooth, Deciduous / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; D007797:Laminin