title: ABCB1 variants confer susceptibility to primary open-angle glaucoma and predict individual differences to latanoprost treatment.
abstract: OBJECTIVE
  We investigated whether ABCB1 variants confer susceptibility to primary open-angle glaucoma and predict individual differences to latanoprost treatment.
  
  
  METHODS
  Between May 2013 and May 2015, 129 POAG patients enrolled in the Department of Ophthalmology, the Second People's Hospital of Yunnan Province were identified as the case group and 121 healthy individuals were included as the control group. Direct DNA sequencing was used to detect four ABCB1 gene polymorphisms, namely, -129T>C (rs3213619), 1236C>T (rs1128503), 2677G>T/A (rs2032582) and 3435C>T (rs1045642). All POAG patients received latanoprost eye drops once daily. The differences in intraocular pressure (IOP) and visual acuity (VA) before and 1 month after latanoprost treatment were compared in different SNPs genotypes.
  
  
  RESULTS
  Statistically significant differences in genotype frequency were found in ABCB1 gene polymorphism 2677G>T/A and 3435C>T between the case group and the control group (both P<0.05). No significant difference in genotype frequency was found in -129T> C and 1236C>T between the two groups (both P>0.05). Importantly, ABCB1 gene 3435C>T polymorphism was associated with a remarkably reduced IOP and an improved VA in POAG patients before and after latanoprost eye drops treatment (both P < 0.05). However, no significant differences in IOP and VA were found in other three genotypes between the two groups (all P>0.05).
  
  
  CONCLUSIONS
  Our results suggest that ABCB1 gene polymorphisms 2677G>T/A and 3435C>T may confer to associated with the susceptibility to POAG. The ABCB1 gene polymorphism 3435C>T may be linked to individual differences in response to latanoprost eye drops treatment in POAG patients.
authors.affiliation: Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming 650000, Yunnan, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming 650000, Yunnan, PR China.
authors.forename: Zhong-Kun
authors.initials: ZK
authors.lastname: Yang
authors.affiliation: Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming 650000, Yunnan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming 650000, Yunnan, PR China.
authors.forename: Wen-Jia
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming 650000, Yunnan, PR China.
authors.forename: Ying-Ting
authors.initials: YT
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, PR China. Electronic address: duanxuanchu_dr@outlook.com.
authors.forename: Xuan-Chu
authors.initials: XC
authors.lastname: Duan
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005902:Glaucoma, Open-Angle / Q000188:drug therapy* / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D000077338:Latanoprost; D008297:Male; D008875:Middle Aged; D009883:Ophthalmic Solutions / Q000627:therapeutic use; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011461:Prostaglandins F, Synthetic / Q000009:adverse effects / Q000627:therapeutic use*; D016896:Treatment Outcome; D014792:Visual Acuity
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D009883:Ophthalmic Solutions; D011461:Prostaglandins F, Synthetic; D000077338:Latanoprost
keywords: ABCB1; Gene polymorphism; Intraocular pressure; Latanoprost; Primary open-angle glaucoma; Response; Susceptibility; Visual acuity