title: A Novel Photosensitizer 3¹,13¹-phenylhydrazine -Mppa (BPHM) and Its in Vitro Photodynamic Therapy against HeLa Cells.
abstract: Photodynamic therapy (PDT) has attracted widespread attention due to its potential in the treatment of various cancers. Porphyrinic pyropheophorbide-a (PPa) has been shown to be a potent photosensitizer in PDT experiments. In this paper, a C-3¹,13¹ bisphenylhydrazone modified methyl pyropheophorbide-a (BPHM) was designed and synthesized with the consideration that phenylhydrazone structure may extend absorption wavelength of methyl pyro-pheophorbide-a (Mppa), and make the photosensitizer potential in deep tumor treatment. The synthesis, spectral properties and in vitro photodynamic therapy (PDT) against human HeLa cervical cancer cell line was studied. Methyl thiazolyl tetrazolium (MTT) assay showed the title compound could achieve strong inhibition of cervical cancer cell viability under visible light (675 nm, 25 J/cm²). Cell uptake experiments were performed on HeLa cells. Morphological changes were examined and analyzed by fluorescent inverted microscope. In addition, the mechanism of the photochemical processes of PDT was investigated, which showed that the formation of singlet oxygen after treatment with PDT played a moderate important role.
authors.affiliation: Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry &amp; Chemical Engineering, Harbin Normal University, Harbin 150025, China. 13039864430@163.com.
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry &amp; Chemical Engineering, Harbin Normal University, Harbin 150025, China. yenghak@hrbnu.edu.cn.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Tan
authors.affiliation: Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry &amp; Chemical Engineering, Harbin Normal University, Harbin 150025, China. 13504800928@163.com.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry &amp; Chemical Engineering, Harbin Normal University, Harbin 150025, China. hsdzls@163.com.
authors.forename: Lishuang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry &amp; Chemical Engineering, Harbin Normal University, Harbin 150025, China. wzq70402@163.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry &amp; Chemical Engineering, Harbin Normal University, Harbin 150025, China. tgh.123@163.com.
authors.forename: Yingxue
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D006367:HeLa Cells; D006801:Humans; D006835:Hydrazones / Q000737:chemistry / Q000627:therapeutic use*; D008353:Mannitol / Q000494:pharmacology; D015394:Molecular Structure; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D011166:Porphyrins / Q000737:chemistry / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism; D019810:Sodium Azide / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; C000615911:C-31,131 bisphenylhydrazone modified methyl pyropheophorbide-a; D006835:Hydrazones; D017319:Photosensitizing Agents; D011166:Porphyrins; D017382:Reactive Oxygen Species; D008353:Mannitol; D019810:Sodium Azide
keywords: BPHM; cell cytotoxicity; photodynamic therapy (PDT)