title: The MTA1 subunit of the nucleosome remodeling and deacetylase complex can recruit two copies of RBBP4/7.
abstract: The nucleosome remodeling and deacetylase (NuRD) complex remodels the genome in the context of both gene transcription and DNA damage repair. It is essential for normal development and is distributed across multiple tissues in organisms ranging from mammals to nematode worms. In common with other chromatin-remodeling complexes, however, its molecular mechanism of action is not well understood and only limited structural information is available to show how the complex is assembled. As a step towards understanding the structure of the NuRD complex, we have characterized the interaction between two subunits: the metastasis associated protein MTA1 and the histone-binding protein RBBP4. We show that MTA1 can bind to two molecules of RBBP4 and present negative stain electron microscopy and chemical crosslinking data that allow us to build a low-resolution model of an MTA1-(RBBP4)2 subcomplex. These data build on our understanding of NuRD complex structure and move us closer towards an understanding of the biochemical basis for the activity of this complex.
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Jason W
authors.initials: JW
authors.lastname: Schmidberger
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sharifi Tabar
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Mario
authors.initials: M
authors.lastname: Torrado
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Ana P G
authors.initials: AP
authors.lastname: Silva
authors.affiliation: School of Chemistry and Molecular Biosciences, University of Queensland, Qld, Australia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Landsberg
authors.affiliation: Institute for Molecular Bioscience, University of Queensland, Qld, Australia.
authors.forename: Lou
authors.initials: L
authors.lastname: Brillault
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Saad
authors.initials: S
authors.lastname: AlQarni
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Yi Cheng
authors.initials: YC
authors.lastname: Zeng
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Parker
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Jason K K
authors.initials: JK
authors.lastname: Low
authors.affiliation: School of Life and Environmental Sciences, University of Sydney, NSW, Australia.
authors.forename: Joel P
authors.initials: JP
authors.lastname: Mackay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry; D015870:Gene Expression; D057809:HEK293 Cells; D006655:Histone Deacetylases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D056505:Retinoblastoma-Binding Protein 4 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D056524:Retinoblastoma-Binding Protein 7 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013816:Thermodynamics; D015534:Trans-Activators; D014158:Transcription, Genetic
chemical_list: D003432:Cross-Linking Reagents; C089320:MTA1 protein, human; D009707:Nucleosomes; D021122:Protein Subunits; C082637:RBBP4 protein, human; C490746:RBBP7 protein, human; D011994:Recombinant Proteins; D012097:Repressor Proteins; D056505:Retinoblastoma-Binding Protein 4; D056524:Retinoblastoma-Binding Protein 7; D015534:Trans-Activators; D006655:Histone Deacetylases
keywords: MTA1; NuRD complex; RBBP4; chromatin; protein structure; transcription regulation