title: Analyses of chicken sialyltransferases related to O-glycosylation.
abstract: The chicken β-galactoside α2,3-sialyltransferase 1, 2, and 5 (ST3Gal1, 2, and 5) genes were cloned, and their enzymes were expressed in 293FT cells. ST3Gal1 and 2 exhibited enzymatic activities toward galactose-β1,3-N-acetylgalactosamine and galactose-β1,3-N-acetylglucosamine. ST3Gal5 only exhibited activity toward lactosylceramide. ST3Gal1 and 2 and previously cloned ST3Gal3 and 6 transferred CMP-sialic acid to asialofetuin. Reverse-transcription-quantitative PCR indicated that ST3Gal1 was expressed at higher levels in the trachea, lung, spleen, and magnum, and the strong expression of ST3Gal5 was observed in the spleen, magnum, and small and large intestines. ST3Gal1, 5, and 6 were also expressed in the tubular gland cells of the magnum, which secretes egg-white proteins. ST3Gal1, 5, and 6 were expressed in the egg chorioallantoic membrane, in which influenza viruses are propagated for the production of vaccines.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kidani
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. Electronic address: kaneoka@nubio.nagoya-u.ac.jp.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kaneoka
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Okuzaki
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Asai
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kojima
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nishijima
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001212:Asialoglycoproteins / Q000378:metabolism; D002460:Cell Line; D002645:Chickens / Q000235:genetics*; D049033:Chorioallantoic Membrane / Q000378:metabolism; D004527:Egg Proteins / Q000378:metabolism; D060748:Fetuins / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006031:Glycosylation; D007790:Lactosylceramides / Q000378:metabolism; D009928:Organ Specificity; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012799:Sialyltransferases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D015703:Antigens, CD; D001212:Asialoglycoproteins; D004527:Egg Proteins; D060748:Fetuins; D007790:Lactosylceramides; D011994:Recombinant Proteins; C017029:asialofetuin; C009744:CDw17 antigen; D012799:Sialyltransferases; D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005690:Galactose; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
keywords: Chicken; Ganglioside; O-Glycosylation; Sialyltransferase; Transgenic chicken