title: A silicon based implantable microelectrode array for electrophysiological and dopamine recording from cortex to striatum in the non-human primate brain.
abstract: Dual-mode, multielectrode recordings have become routine in rodent neuroscience research and have recently been adapted to the non-human primate. However, robust and reliable application of acute, multielectrode recording methods in monkeys especially for deep brain nucleus research remains a challenge. In this paper, We described a low cost silicon based 16-site implantable microelectrode array (MEA) chip fabricated by standard lithography technology for in vivo test. The array was 25mm long and designed to use in non-human primate models, for electrophysiological and electrochemical recording. We presented a detailed protocol for array fabrication, then showed that the device can record Spikes, LFPs and dopamine (DA) variation continuously from cortex to striatum in an esthetized monkey. Though our experiment, high-quality electrophysiological signals were obtained from the animal. Across any given microelectrode, spike amplitudes ranged from 70 to 300μV peak to peak, with a mean signal-to-noise ratio of better than 5:1. Calibration results showed the MEA probe had high sensitivity and good selectivity for DA. The DA concentration changed from 42.8 to 481.6μM when the MEA probe inserted from cortex into deep brain nucleus of striatum, which reflected the inhomogeneous distribution of DA in brains. Compared with existing methods allowing single mode (electrophysiology or electrochemistry) recording. This system is designed explicitly for dual-mode recording to meet the challenges of recording in non-human primates.
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Song
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Mixia
authors.initials: M
authors.lastname: Wang
authors.affiliation: University of Kentucky Chandler Medical Center, Lexington, KY 40536-0098, USA.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
authors.forename: Xianteng
authors.initials: X
authors.lastname: Song
authors.affiliation: Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhuang
authors.affiliation: Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yue
authors.affiliation: Xuanwu Hospital, Capital Medical University, Beijing 100053, China. Electronic address: pbchan90@gmail.com.
authors.forename: Piu
authors.initials: P
authors.lastname: Chan
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China. Electronic address: xxcai@mail.ie.ac.cn.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Cai
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D001921:Brain / Q000502:physiology*; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000737:chemistry / Q000502:physiology; D003342:Corpus Striatum / Q000737:chemistry / Q000502:physiology; D004298:Dopamine / Q000032:analysis* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000295:instrumentation; D004567:Electrodes, Implanted; D055724:Electrophysiological Phenomena; D004867:Equipment Design; D000882:Haplorhini; D006801:Humans; D008297:Male; D008839:Microelectrodes; D012825:Silicon / Q000737:chemistry*
chemical_list: D004298:Dopamine; D012825:Silicon
keywords: DA; Implantable MEA; Neurochemical; Neuroelectrical; Non-human primate