title: Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis.
abstract: Diagnosis of Opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. Therefore sensitive and specific diagnostic test is needed. In this study, a coproantigen sandwich ELISA using recombinant O. viverrini cathepsin F (rOv-CF) was developed. This sandwich ELISA employing chicken IgY raised against rOv-CF in combination with rabbit IgG antibody to the somatic O. viverrini antigens showed a lower detection limit (LLD) of 70ng native O. viverrini somatic antigens by spiking the parasite antigens into control feces. When applied to the diagnosis, the IgY-based sandwich ELISA exhibited sensitivity and specificity of 93.3% and 76.7%, respectively, in an investigation of 90 human cases positive or negative for opisthorchiasis. The positive predictive value (PPV) and negative predictive value (NPV) for this coproantigen detection were 66.7% and 95.2%, respectively. This IgY-based sandwich ELISA using parasite cathepsin F detection shows a promising immunodiagnostic alternative for human opisthorchiasis in endemic regions.
authors.affiliation: Centre of Research Excellence for Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Bangkok 10700, Thailand; WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Salma
authors.initials: S
authors.lastname: Teimoori
authors.affiliation: WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Arimatsu
authors.affiliation: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Thewarach
authors.initials: T
authors.lastname: Laha
authors.affiliation: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Sasithorn
authors.initials: S
authors.lastname: Kaewkes
authors.affiliation: Department of Pathobiology, Faculty of Veterinary, Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Piya
authors.initials: P
authors.lastname: Sereerak
authors.affiliation: WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Manop
authors.initials: M
authors.lastname: Sripa
authors.affiliation: Department of Pathobiology, Faculty of Veterinary, Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Sirikachorn
authors.initials: S
authors.lastname: Tangkawattana
authors.affiliation: Department of Microbiology, Immunology and Tropical Medicine, and Research Center for Neglected Diseases of Poverty, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Brindley
authors.affiliation: Centre of Research Excellence for Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Bangkok 10700, Thailand. Electronic address: banchob@kku.ac.th.
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism*; D056646:Cathepsin F / Q000378:metabolism; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D015801:Helminth Proteins / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D009889:Opisthorchiasis / Q000175:diagnosis* / Q000469:parasitology; D009891:Opisthorchis / Q000302:isolation & purification*; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; C016512:IgY; D007136:Immunoglobulins; D056646:Cathepsin F
keywords: Cathepsin F; Chicken IgY; Coproantigen; Immunodiagnosis; Opisthorchis viverrini; Sandwich ELISA