title: MicroRNA-300 inhibited glioblastoma progression through ROCK1.
abstract: Glioblastoma is a common type of brain aggressive tumors and has a poor prognosis. MicroRNAs (miRNAs) are a class of small, endogenous and non-coding RNAs that play crucial roles in cell proliferation, survival and invasion. Deregulated expression of miR-300 has been studied in a lot of cancers. However, the role of miR-300 in glioblastoma is still unknown. In this study, we demonstrated that miR-300 expression was downregulated in glioblastoma tissues compared with the normal tissues. Lower expression level of miR-300 was observed in thirty cases (75 %, 30/40) of glioblastoma samples compared with the normal samples. Moreover, the overall survival of glioblastoma patients with lower miR-300 expression level was shorter than those with higher miR-300 expression level. In addition, miR-300 expression was also downregulated in glioblastoma cell lines. Overexpression of miR-300 inhibited cell proliferation, cell cycle and invasion in glioblastoma cell line U87 and U251. Moreover, we identified ROCK1 as a direct target of miR-300 in U87 and U251 cells. Overexpression of ROCK1 partially rescued the miR-300-mediated cell growth. ROCK1 expression levels in glioblastoma tissues were higher than that in normal tissues. ROCK1 expression levels were higher in thirty-one cases of glioblastoma samples than their normal samples. Furthermore, the expression level ROCK1 was inversely correlated with the expression level of miR-300. Importantly, overexpression of miR-300 suppressed glioblastoma progression in an established xenograft model. In conclusion, we revealed that miR-300 might act as a tumor suppressor gene through inhibiting ROCK1 in glioblastoma.
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Fucheng
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Xuanxi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Zuobin
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Jianjiao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Yongri
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Harbin Medical University, Harbin 150086, China.
authors.forename: Deli
authors.initials: D
authors.lastname: Dong
authors.affiliation: Institute of Molecular Biology, Austrian Academy of Sciences, Vienna, Austria.
authors.forename: Shan
authors.initials: S
authors.lastname: Bian
authors.affiliation: Harbin Medical University, Harbin 150086, China.
authors.forename: Baofeng
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Chuanlu
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014183:Transplantation, Heterologous; D054460:rho-Associated Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C000596512:MIRN300 microRNA, human; D035683:MicroRNAs; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases
keywords: ROCK1; glioblastoma; miR-300; microRNAs