title: Moesin regulates neutrophil rolling velocity in vivo.
abstract: During inflammation, the selectin-induced slow rolling of neutrophils on venules cooperates with chemokine signaling to mediate neutrophil recruitment into tissues. Previous studies identified P-selectin glycoprotein ligand-1 (PSGL-1) and CD44 as E-selectin ligands that activate integrins to induce slow rolling. We show here that in TNF-α-treated cremaster muscle venules, slow leukocyte rolling was impaired in mice deficient in moesin, a member of the ezrin-radixin-moesin (ERM) family. Accordingly, neutrophil recruitment in a peritonitis model was decreased in moesin-deficient mice when chemokine signaling was blocked with pertussis toxin. These results suggest that moesin contributes to the slow rolling and subsequent recruitment of neutrophils during inflammation.
authors.affiliation: Laboratory of Combined Research on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsumoto
authors.affiliation: Department of Fundamental Biosciences, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan. Electronic address: tahirata@belle.shiga-med.ac.jp.
authors.forename: Takako
authors.initials: T
authors.lastname: Hirata
mesh_terms: D000009:Abdominal Muscles / Q000098:blood supply; D000818:Animals; D002465:Cell Movement / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology*; D010538:Peritonitis / Q000276:immunology*; D015398:Signal Transduction; D014699:Venules / Q000276:immunology*
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; C071058:moesin; C071776:radixin
keywords: Ezrin; Migration; Moesin; Neutrophils; Radixin; Rolling