title: Pichia kudriavzevii as a representative yeast of North Patagonian winemaking terroir.
abstract: Terroir concept includes specific soil, topography, climate, landscape characteristics and biodiversity features. In reference to the last aspect, recent studies investigating the microbial biogeography (lately called 'microbial terroir') have revealed that different wine-growing regions maintain different microbial communities. The aim of the present work was to identify potential autochthonous fermentative yeasts isolated from native plants in North Patagonia, Schinus johnstonii, Ephedra ochreata and Lycium chilense, that could be associated to the specific vitivinicultural terroir of this region. Different Pichia kudriavzevii isolates were recovered from these plants and physiologically and genetically compared to regional wine isolates and foreign reference strains of the same species. All isolates were subjected to molecular characterization including mtDNA-RFLP, RAPD-PCR and sequence analysis. Both wine and native P. kudriavzevii isolates from Patagonia showed similar features, different from those showed by foreign strains, suggesting that this species could be part of a specific regional terroir from North Patagonia.
authors.affiliation: Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas (PROBIEN, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina - Universidad Nacional del Comahue), Buenos Aires, Neuquén, Argentina; Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Argentina.
authors.forename: Silvana M
authors.initials: SM
authors.lastname: Del Mónaco
authors.affiliation: Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas (PROBIEN, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina - Universidad Nacional del Comahue), Buenos Aires, Neuquén, Argentina; Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Argentina. Electronic address: meugenia.rodriguez@probien.gob.ar.
authors.forename: María E
authors.initials: ME
authors.lastname: Rodríguez
authors.affiliation: Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas (PROBIEN, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina - Universidad Nacional del Comahue), Buenos Aires, Neuquén, Argentina; Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Argentina.
authors.forename: Christian A
authors.initials: CA
authors.lastname: Lopes
mesh_terms: D027926:Anacardiaceae / Q000382:microbiology*; D001118:Argentina; D044822:Biodiversity; D002677:Chile; D004272:DNA, Mitochondrial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D029789:Ephedra / Q000382:microbiology*; D005285:Fermentation / Q000502:physiology*; D032305:Lycium / Q000382:microbiology*; D010843:Pichia / Q000235:genetics* / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014920:Wine / Q000032:analysis / Q000382:microbiology*
chemical_list: D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer
keywords: Ephedra ochreata; Lycium chilense; Wine: Schinus johnstonii; Yeast diversity