title: Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria.
abstract: Hydrogen was produced from steam-exploded corn stover by using a combination of the cellulolytic bacterium Clostridium cellulolyticum and non-cellulolytic hydrogen-producing bacteria. The highest hydrogen yield of the co-culture system with C. cellulolyticum and Citrobacter amalonaticus reached 51.9 L H2/kg total solid (TS). The metabolites from the co-culture system were significantly different from those of the mono-culture systems. Formate, which inhibits the growth of C. cellulolyticum, could be consumed by the hydrogen-evolving bacteria, and transformed into hydrogen. Glucose and xylose were released from corn stover via hydrolysis by C. cellulolyticum and were quickly utilized in dark fermentation with the co-cultured hydrogen-producing bacteria. Because the hydrolysis of corn stover by C. cellulolyticum was much slower than the utilization of glucose and xylose by the hydrogen-evolving bacteria, the sugar concentrations were always maintained at low levels, which favored a high hydrogen molar yield.
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Shou-Chi
authors.initials: SC
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Qi-Heng
authors.initials: QH
authors.lastname: Lai
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Zhi-Dan
authors.initials: ZD
authors.lastname: Liu
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Tian-Min
authors.initials: TM
authors.lastname: Wang
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China. Electronic address: chongzhang@mail.tsinghua.edu.cn.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Xing
mesh_terms: D018533:Biomass; D002482:Cellulose / Q000378:metabolism; D002954:Citrobacter / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D046973:Ruminiclostridium cellulolyticum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018920:Coculture Techniques; D005285:Fermentation; D005561:Formates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006868:Hydrolysis; D013227:Steam; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005561:Formates; D013227:Steam; C030544:formic acid; D006859:Hydrogen; D002482:Cellulose; D014994:Xylose; D005947:Glucose
keywords: Corn stover; Dark fermentation; Hydrogen production; Mesophilic co-culture; Synergic effect