title: A "Hit and Run" Approach to Inducible Direct Reprogramming of Astrocytes to Neural Stem Cells.
abstract: Temporal and spatial control of gene expression can be achieved using an inducible system as a fundamental tool for regulated transcription in basic, applied and eventually in clinical research. We describe a novel "hit and run" inducible direct reprogramming approach. In a single step, 2 days post-transfection, transiently transfected Sox2(FLAG) under the Leu3p-αIPM inducible control (iSox2) triggers the activation of endogenous Sox2, redirecting primary astrocytes into abundant distinct nestin-positive radial glia cells. This technique introduces a unique novel tool for safe, rapid and efficient reprogramming amendable to regenerative medicine.
authors.affiliation: Stem Cell Biology Laboratory, Biomedical Sciences Research Centre "Alexander Fleming," Vari-Attica, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Poulou
authors.affiliation: Stem Cell Biology Laboratory, Biomedical Sciences Research Centre "Alexander Fleming,"Vari-Attica, Greece; Choremio Laboratory, Department of Pediatrics, National University of Athens Medical SchoolAthens, Greece.
authors.forename: Nikolaos P
authors.initials: NP
authors.lastname: Mandalos
authors.affiliation: Stem Cell Biology Laboratory, Biomedical Sciences Research Centre "Alexander Fleming,"Vari-Attica, Greece; Choremio Laboratory, Department of Pediatrics, National University of Athens Medical SchoolAthens, Greece.
authors.forename: Theodoros
authors.initials: T
authors.lastname: Karnavas
authors.affiliation: Stem Cell Biology Laboratory, Biomedical Sciences Research Centre "Alexander Fleming," Vari-Attica, Greece.
authors.forename: Marannia
authors.initials: M
authors.lastname: Saridaki
authors.affiliation: Basic Sciences Division, The Lieber Institute for Brain Development Baltimore, MD, USA.
authors.forename: Ronald D G
authors.initials: RD
authors.lastname: McKay
authors.affiliation: Stem Cell Biology Laboratory, Biomedical Sciences Research Centre "Alexander Fleming,"Vari-Attica, Greece; Choremio Laboratory, Department of Pediatrics, National University of Athens Medical SchoolAthens, Greece; Basic Sciences Division, The Lieber Institute for Brain DevelopmentBaltimore, MD, USA.
authors.forename: Eumorphia
authors.initials: E
authors.lastname: Remboutsika
keywords: CREERT2; FLP; doxycycline; embryonic stem cells; induced pluripotent stem cells; neural progenitor cells; tetracycline; tissue regeneration