title: Tinospora cordifolia ameliorates anxiety-like behavior and improves cognitive functions in acute sleep deprived rats.
abstract: Sleep deprivation (SD) leads to the spectrum of mood disorders like anxiety, cognitive dysfunctions and motor coordination impairment in many individuals. However, there is no effective pharmacological remedy to negate the effects of SD. The current study examined whether 50% ethanolic extract of Tinospora cordifolia (TCE) can attenuate these negative effects of SD. Three groups of adult Wistar female rats - (1) vehicle treated-sleep undisturbed (VUD), (2) vehicle treated-sleep deprived (VSD) and (3) TCE treated-sleep deprived (TSD) animals were tested behaviorally for cognitive functions, anxiety and motor coordination. TSD animals showed improved behavioral response in EPM and NOR tests for anxiety and cognitive functions, respectively as compared to VSD animals. TCE pretreatment modulated the stress induced-expression of plasticity markers PSA-NCAM, NCAM and GAP-43 along with proteins involved in the maintenance of LTP i.e., CamKII-α and calcineurin (CaN) in hippocampus and PC regions of the brain. Interestingly, contrary to VSD animals, TSD animals showed downregulated expression of inflammatory markers such as CD11b/c, MHC-1 and cytokines along with inhibition of apoptotic markers. This data suggests that TCE alone or in combination with other memory enhancing agents may help in managing sleep deprivation associated stress and improving cognitive functions.
authors.affiliation: Medical Biotechnology Laboratory, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab-143005, INDIA.
authors.forename: Rachana
authors.initials: R
authors.lastname: Mishra
authors.affiliation: Medical Biotechnology Laboratory, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab-143005, INDIA.
authors.forename: Shaffi
authors.initials: S
authors.lastname: Manchanda
authors.affiliation: Medical Biotechnology Laboratory, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab-143005, INDIA.
authors.forename: Muskan
authors.initials: M
authors.lastname: Gupta
authors.affiliation: Medical Biotechnology Laboratory, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab-143005, INDIA.
authors.forename: Taranjeet
authors.initials: T
authors.lastname: Kaur
authors.affiliation: Medical Biotechnology Laboratory, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab-143005, INDIA.
authors.forename: Vedangana
authors.initials: V
authors.lastname: Saini
authors.affiliation: Medical Biotechnology Laboratory, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab-143005, INDIA.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Medical Biotechnology Laboratory, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab-143005, INDIA.
authors.forename: Gurcharan
authors.initials: G
authors.lastname: Kaur
mesh_terms: D000208:Acute Disease; D000818:Animals; D001007:Anxiety / Q000517:prevention & control*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003071:Cognition / Q000187:drug effects*; D000431:Ethanol / Q000737:chemistry; D005260:Female; D006120:Grooming / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D018782:Maze Learning / Q000187:drug effects; D009043:Motor Activity / Q000235:genetics; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000503:physiopathology / Q000517:prevention & control* / Q000523:psychology; D031610:Tinospora / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D010936:Plant Extracts; D000431:Ethanol