title: Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain.
abstract: Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed transcriptome analyses on murine iNSCs in comparison to brain-derived neural stem cells (NSCs) and pluripotent stem cell-derived NPCs, which revealed distinct global, neural, metabolic and cell cycle-associated marks in these populations. iNSCs carried a hindbrain/posterior cell identity, which could be shifted towards caudal, partially to rostral but not towards ventral fates in vitro. iNSCs survived after transplantation into the rodent brain and exhibited in vivo-characteristics, neural and metabolic programs similar to transplanted NSCs. However, iNSCs vastly retained caudal identities demonstrating cell-autonomy of regional programs in vivo. These data could have significant implications for a variety of in vitro- and in vivo-applications using iNSCs.
authors.affiliation: Institute of Neuropathology, University Hospital Münster, 48149 Münster, Germany; Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Anna-Lena
authors.initials: AL
authors.lastname: Hallmann
authors.affiliation: Group of Computational Biology and Systems Biomedicine, Biodonostia Health Research Institute, 20014 San Sebastián, Spain; IKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain.
authors.forename: Marcos J
authors.initials: MJ
authors.lastname: Araúzo-Bravo
authors.affiliation: Institute of Neuropathology, University Hospital Münster, 48149 Münster, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Zerfass
authors.affiliation: Institute of Neuropathology, University Hospital Münster, 48149 Münster, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Senner
authors.affiliation: Institute of Neuropathology, University Hospital Münster, 48149 Münster, Germany; Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Ehrlich
authors.affiliation: Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Olympia E
authors.initials: OE
authors.lastname: Psathaki
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, 143701 Seoul, Republic of Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Han
authors.affiliation: Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany; Institute of Biomedicine of Valencia, Spanish National Research Council (IBV-CSIC), Jaime Roig 11, 46010 Valencia, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Tapia
authors.affiliation: Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Holm
authors.initials: H
authors.lastname: Zaehres
authors.affiliation: Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.
authors.forename: Hans R
authors.initials: HR
authors.lastname: Schöler
authors.affiliation: Institute of Neuropathology, University Hospital Münster, 48149 Münster, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Kuhlmann
authors.affiliation: Institute of Neuropathology, University Hospital Münster, 48149 Münster, Germany; Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany; Department of Pathology and Cell Biology, Columbia University Medical Center, 10032 New York, USA. Electronic address: gunnar.hargus@ukmuenster.de.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Hargus
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D016000:Cluster Analysis; D005347:Fibroblasts / Q000166:cytology; D020869:Gene Expression Profiling; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D014157:Transcription Factors
keywords: Induced neural stem cells; Neural stem cells; Regional programs; Transcriptome; Transplantation