title: A novel enzyme-assisted ultrasonic approach for highly efficient extraction of resveratrol from Polygonum cuspidatum.
abstract: Resveratrol is a promising multi-biofunctional phytochemical, which is abundant in Polygonum cuspidatum. Several methods for resveratrol extraction have been reported, while they often take a long extraction time accompanying with poor extraction yield. In this study, a novel enzyme-assisted ultrasonic approach for highly efficient extraction of resveratrol from P. cuspidatum was developed. According to results, the resveratrol yield significantly increased after glycosidases (Pectinex® or Viscozyme®) were applied in the process of extraction, and better extraction efficacy was found in the Pectinex®-assisted extraction compared to Viscozyme®-assisted extraction. Following, a 5-level-4-factor central composite rotatable design with response surface methodology (RSM) and artificial neural network (ANN) was selected to model and optimize the Pectinex®-assisted ultrasonic extraction. Based on the coefficient of determination (R(2)) calculated from the design data, ANN model displayed much more accurate in data fitting as compared to RSM model. The optimum conditions for the extraction determined by ANN model were substrate concentration of 5%, acoustic power of 150W, pH of 5.4, temperature of 55°C, the ratio of enzyme to substrate of 3950 polygalacturonase units (PGNU)/g of P. cuspidatum, and reaction time of 5h, which can lead to a significantly high resveratrol yield of 11.88mg/g.
authors.affiliation: Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 40227, Taiwan.
authors.forename: Jer-An
authors.initials: JA
authors.lastname: Lin
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, 142 Haijhuan Road, Nanzih District, Kaohsiung 81157, Taiwan.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Kuo
authors.affiliation: Graduate Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 40227, Taiwan.
authors.forename: Bao-Yuan
authors.initials: BY
authors.lastname: Chen
authors.affiliation: Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 40227, Taiwan.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan.
authors.forename: Yung-Chuan
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Graduate Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 40227, Taiwan.
authors.forename: Jiann-Hwa
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 40227, Taiwan. Electronic address: cjshieh@nchu.edu.tw.
authors.forename: Chwen-Jen
authors.initials: CJ
authors.lastname: Shieh
mesh_terms: D045468:Fallopia japonica / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry*; D013696:Temperature; D014465:Ultrasonics
chemical_list: D013267:Stilbenes; D000077185:Resveratrol
keywords: Artificial neural network; Enzyme-assisted ultrasonic approach; Polygonum cuspidatum; Response surface methodology; Resveratrol