title: EBNA1 antigen-specific CD8+ T cells in cerebrospinal fluid of patients with multiple sclerosis.
abstract: Epidemiological data suggests that Epstein-Barr virus may be involved in the pathogenesis of Multiple Sclerosis (MS). We aimed to determine the frequency of CD8+ T cells specific for one EBNA1-derived epitope (HPVGEADYFEY) in cerebrospinal fluid (CSF) and blood of patients with MS and other inflammatory neurological diseases (OIND). The frequency of specific CD8+ T cells was assessed by HLA-class-I-binding pentamers restricted to HLA-B35. The frequency of HPVGEADYFEY-specific CD8+ T cells did neither differ significantly in blood nor CSF in MS compared to OIND, but was consistently higher in CSF compared to blood regardless of diagnosis.
authors.affiliation: Department of Neurology, Charité-Universitätsmedizin Berlin, Germany. Electronic address: hebun.erdur@charite.de.
authors.forename: Hebun
authors.initials: H
authors.lastname: Erdur
authors.affiliation: Department of Neurology, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Scholz
authors.affiliation: Institute of Medical Immunology, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Streitz
authors.affiliation: Department of Nephrology and Internal Intensive Care, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Hammer
authors.affiliation: Institute of Medical Immunology, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Meisel
authors.affiliation: Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Schönemann
authors.affiliation: Institute of Clinical Chemistry, University Medical-Centre Schleswig-Holstein, Campus Lübeck, Germany; Department of Neurology, University Medical-Centre Schleswig-Holstein, Campus Lübeck, Germany.
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Wandinger
authors.affiliation: Department of Neurology, Charité-Universitätsmedizin Berlin, Germany.
authors.forename: Berit
authors.initials: B
authors.lastname: Rosche
mesh_terms: D000906:Antibodies / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000134:cerebrospinal fluid* / Q000150:complications; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid* / Q000150:complications / Q000821:virology
chemical_list: D000906:Antibodies; D019309:Epstein-Barr Virus Nuclear Antigens; C417029:EBV-encoded nuclear antigen 1
keywords: Cerebrospinal fluid; Epstein–Barr virus; Multiple sclerosis