title: [Comparison of the Conventional Centrifuged and Filtrated Preparations in Urine Cytology].
abstract: The urine cytology test is one of the most important tools for the diagnosis of malignant urinary tract tumors. This test is also of great value for predicting malignancy. However, the sensitivity of this test is not high enough to screen for malignant cells. In our laboratory, we were able to attain a high sensitivity of urine cytology tests after changing the preparation method of urine samples. The differences in the cytodiagnosis between the two methods are discussed here. From January 2012 to June 2013, 2,031 urine samples were prepared using the conventional centrifuge method (C method) ; and from September 2013 to March 2015, 2,453 urine samples were prepared using the filtration method (F method) for the cytology test. When the samples included in category 4 or 5, were defined as cytological positive, the sensitivities of this test with samples prepared using the F method were significantly high compared with samples prepared using the C method (72% vs 28%, p＜0.001). The number of cells on the glass slides prepared by the F method was significantly higher than that of the samples prepared by the C method (p＜0.001). After introduction of the F method, the number of f alse negative cases was decreased in the urine cytology test because a larger number of cells was seen and easily detected as atypical or malignant epithelial cells. Therefore, this method has a higher sensitivity than the conventional C method as the sensitivity of urine cytology tests relies partially on the number of cells visualized in the prepared samples.
authors.affiliation: The Department of Urology, Chibaken Saiseikai Narashino Hospial.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Sekita
authors.affiliation: The Division of Pathology, Chibaken Saiseikai Narashino Hospital.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shimosakai
authors.affiliation: The Department of Urology, Graduate School of Medicine, Chiba University.
authors.forename: Rika
authors.initials: R
authors.lastname: Nishikawa
authors.affiliation: The Department of Urology, Chibaken Saiseikai Narashino Hospial.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sato
authors.affiliation: The Department of Urology, Chibaken Saiseikai Narashino Hospial.
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Kouno
authors.affiliation: The Department of Urology, Chibaken Saiseikai Narashino Hospial.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Fujimura
authors.affiliation: The Department of Urology, Chibaken Saiseikai Narashino Hospial.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Mikami
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002498:Centrifugation; D003581:Cytodiagnosis / Q000379:methods*; D005188:False Negative Reactions; D005260:Female; D005374:Filtration; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D014556:Urine / Q000166:cytology*; D014571:Urologic Neoplasms / Q000175:diagnosis* / Q000652:urine*