title: Clinical significance of ADAMTS1, ADAMTS5, ADAMTS9 aggrecanases and IL-17A, IL-23, IL-33 cytokines in polycystic ovary syndrome.
abstract: OBJECTIVE
  Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women of reproductive age. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS) are involved in inflammation and fertility. The aim of this investigation was to evaluate the serum levels of ADAMTS1, ADAMTS5, ADAMTS9, IL-17, IL-23, IL-33 and to find out the relationship between these inflammatory cytokines and ADAMTSs in PCOS patients.
  
  
  METHODS
  A case-control study was performed in a training and research hospital. Eighty patients with PCOS and seventy-eight healthy female volunteers were recruited in the present study. Serum ADAMTS and IL levels were determined by a human enzyme-linked immunoassay (ELISA) in all subjects.
  
  
  RESULTS
  The IL-17A, IL-23 and IL-33 levels were significantly higher in the PCOS patients compared to the controls (p < 0.05). We could not find significant difference between the groups in terms of ADAMTS1, ADAMTS5 and ADAMTS9 levels. IL-17A had positive correlations with LDL cholesterol and IL-33 and negative correlations with ADAMTS1, ADAMTS5, and ADAMTS9. IL-33 had positive correlation with LDL cholesterol and IL-17A. In ROC curve analysis, PCOS can be predicted by the use of IL-17A, IL-23 and IL-33 which at a cut-off value of 8.37 pg/mL (44 % sensitivity, 83 % specificity), 26.75 pg/mL (36 % sensitivity, 64 % specificity) and 14.28 pg/mL (83 % sensitivity, 39 % specificity), respectively.
  
  
  CONCLUSIONS
  The results of the study might suggest that ADAMTS and IL molecules have a role in the pathogenesis of the PCOS. Further efforts are needed to establish causality for ADAMTS-IL axis.
authors.affiliation: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Irfan Bastug Caddesi, 06110, Ankara, Turkey. meliakarakose@yahoo.com.
authors.forename: M
authors.initials: M
authors.lastname: Karakose
authors.affiliation: Department of Medical Biology, Turgut Ozal University School of Medicine, Gimat, Ankara, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Demircan
authors.affiliation: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Irfan Bastug Caddesi, 06110, Ankara, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Tutal
authors.affiliation: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Irfan Bastug Caddesi, 06110, Ankara, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Demirci
authors.affiliation: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Irfan Bastug Caddesi, 06110, Ankara, Turkey.
authors.forename: M S
authors.initials: MS
authors.lastname: Arslan
authors.affiliation: Department of Endocrinology and Metabolism, Ankara University, School of Medicine, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Sahin
authors.affiliation: Department of Biochemistry, Turgut Ozal University School of Medicine, Gimat, Ankara, Turkey.
authors.forename: H T
authors.initials: HT
authors.lastname: Celik
authors.affiliation: Department of Biochemistry, Turgut Ozal University School of Medicine, Gimat, Ankara, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Kazanci
authors.affiliation: Department of Biostatistics, Faculty of Medicine, Hacettepe University, Sihhiye, Ankara, Turkey.
authors.forename: J
authors.initials: J
authors.lastname: Karakaya
authors.affiliation: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Irfan Bastug Caddesi, 06110, Ankara, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Cakal
authors.affiliation: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Irfan Bastug Caddesi, 06110, Ankara, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Delibasi
mesh_terms: D000071097:ADAMTS1 Protein / Q000097:blood*; D000071116:ADAMTS5 Protein / Q000097:blood*; D000071119:ADAMTS9 Protein / Q000097:blood*; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000097:blood*; D053759:Interleukin-23 / Q000097:blood*; D000067596:Interleukin-33 / Q000097:blood*; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; C508717:IL17A protein, human; C506536:IL33 protein, human; D020381:Interleukin-17; D053759:Interleukin-23; D000067596:Interleukin-33; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human; D000071119:ADAMTS9 Protein; C412869:ADAMTS9 protein, human
keywords: ADAMTS; Insulin resistance; Interleukin; Polycystic ovary syndrome