title: Preparation of imprinted cryogel cartridge for chiral separation of l-phenylalanine.
abstract: l-Phe-imprinted cryogel cartridge was prepared for the chiral separation of l-Phe. N-Methacryloyl l-phenylalanine (MAPA) was used as a functional monomer for complexing with l-Phe. The selectivity of the membranes was investigated by using d-Phe, l-Trp, and d-Trp as competitor molecules. The PHEMAPA-l-Trp membranes were 6.4, 4.3, and 5.5 times more selective for l-Phe than d-Phe, l-Trp, and d-Trp, respectively. The PHEMAPA-l-Phe cryogel cartridge was incorporated into the fast protein liquid chromatography (FPLC) equipment and was able to separate D,l-Phe racemic mixture efficiently. The PHEMAPA-l-Phe membranes were shown to be reusable many times without significant loss of the adsorption capacity.
authors.affiliation: a Department of Chemistry , Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Semra
authors.initials: S
authors.lastname: Akgönüllü
authors.affiliation: a Department of Chemistry , Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Handan
authors.initials: H
authors.lastname: Yavuz
authors.affiliation: a Department of Chemistry , Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D059985:Cryogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D054802:Molecular Imprinting; D009994:Osmolar Concentration; D010649:Phenylalanine / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D059985:Cryogels; D014867:Water; D010649:Phenylalanine
keywords: Affinity cryogel; PHEMA; amino acid imprinting; chiral separation; l-phenylalanine