title: Ratiometric two-photon excited photoluminescence of quantum dots triggered by near-infrared-light for real-time detection of nitric oxide release in situ.
abstract: Probe-donor integrated nanocomposites were developed from conjugating silica-coated Mn(2+):ZnS quantum dots (QDs) with MoS2 QDs and photosensitive nitric oxide (NO) donors (Fe4S3(NO)7(-), RBS). Under excitation with near-infrared (NIR) light at 808 nm, the Mn(2+):ZnS@SiO2/MoS2-RBS nanocomposites showed the dual-emissive two-photon excited photoluminescence (TPEPL) that induced RBS photolysis to release NO in situ. NO caused TPEPL quenching of Mn(2+):ZnS QDs, but it produced almost no impact on the TPEPL of MoS2 QDs. Hence, the nanocomposites were developed as a novel QDs-based ratiometric TPEPL probe for real-time detection of NO release in situ. The ratiometric TPEPL intensity is nearly linear (R(2) = 0.9901) with NO concentration in the range of 0.01∼0.8 μM, which corresponds to the range of NO release time (0∼15 min). The detection limit was calculated to be approximately 4 nM of NO. Experimental results confirmed that this novel ratiometric TPEPL probe possessed high selectivity and sensitivity for the detection of NO against potential competitors, and especially showed high detection performance for NIR-light triggered NO release in tumor intracellular microenvironments. These results would promote the development of versatile probe-donor integrated systems, also providing a facile and efficient strategy to real-time detect the highly controllable drug release in situ, especially in physiological microenvironments.
authors.affiliation: Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, College of Chemistry and Chemical Engineering, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, College of Chemistry and Chemical Engineering, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China. Electronic address: guirijun@qdu.edu.cn.
authors.forename: Rijun
authors.initials: R
authors.lastname: Gui
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, PR China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D057230:Limit of Detection; D049449:Luminescence; D053761:Nanocomposites; D009569:Nitric Oxide / Q000032:analysis*; D017785:Photons; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods; D059016:Tumor Microenvironment
chemical_list: D005456:Fluorescent Dyes; D009569:Nitric Oxide
keywords: Near-infrared; Nitric oxide; Quantum dots; Ratiometric; Two-photon photoluminescence