title: Anti prostate cancer using PEGylated bombesin containing, cabazitaxel loading nano-sized drug delivery system.
abstract: BACKGROUND
  Prostate cancer (PCa) is the second most-frequently diagnosed cancer in men. Cabazitaxel was approved for the treatment of patients with hormone-refractory metastatic prostate cancer previously treated with a docetaxel-containing regimen.
  
  
  OBJECTIVE
  In this study, bombesin (BN), a ligand reported to specifically target GRP overexpressing prostate tumor, was applied for the construction of lipid-polymer hybrid nanoparticles (LPNs), and used for the targeted delivery of cabazitaxel (CAB) to prostate cancer.
  
  
  METHODS
  BN-polyethylene glycol-1,2-Distearoyl-sn-glycero-3-phosphoethanolamine (BN-PEG-DSPE) was synthesized. CAB loaded, BN-PEG-DSPE contained LPNs (BN-CAB-LPNs) were prepared. Their particle size, zeta potential and drug encapsulation efficiency (EE) were evaluated. In vitro cytotoxicity study of BN-CAB-LPNs was tested in LNCaP human prostatic cancer cell line (LNCaP cells). In vivo anti-tumor efficacy of the carriers was evaluated on mice bearing prostate cancer model.
  
  
  RESULTS
  The optimum BN-CAB-LPNs formulations had a particle size of 184.9 nm and a 26.5 mV positive surface charge. The growth of LNCaP cells in vitro was obviously inhibited. BN-CAB-LPNs also displayed better anti-tumor activity than the other formulations in vivo.
  
  
  CONCLUSIONS
  The results demonstrated that BN-CAB-LPNs can sufficiently deliver CAB to the cancer cells and enhance the anti-tumor capacity. Thus, BN-CAB-LPNs can be proved to be a superior nanomedicine which can achieve better therapeutic efficacy of prostate tumor.
authors.affiliation: a Department of Oncology , Linyi People's Hospital , Linyi , Shandong , P. R. China ;
authors.forename: Wenjun
authors.initials: W
authors.lastname: Chen
authors.affiliation: b Department of Gerontology , Linyi People's Hospital , Linyi , Shandong , P. R. China.
authors.forename: Miao
authors.initials: M
authors.lastname: Guo
authors.affiliation: b Department of Gerontology , Linyi People's Hospital , Linyi , Shandong , P. R. China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Wang
keywords: Bombesin; PEGylated; cabazitaxel; nano-sized drug delivery system; prostate cancer