title: Sensitive electrochemical detection of rutin and isoquercitrin based on SH-β-cyclodextrin functionalized graphene-palladium nanoparticles.
abstract: In this study, a sensitive electrochemical method based on thio-β-cyclodextrin functionalized graphene/palladium nanoparticles (SH-β-CD-Gr/PdNPs) was developed to detect rutin and isoquercitrin, respectively. The obtained SH-β-CD-Gr/PdNPs were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The results confirm that SH-β-CD and PdNPs were successfully deposited on the surface of graphene nanosheets (GNs). The peak currents of rutin and isoquercitrin on the SH-β-CD-Gr/PdNPs modified glassy carbon electrode (GCE) are dramatically increased compared with that on the bare GCE and SH-β-CD-Gr/GCE. It indicated that the nanocomposite integrated the excellent electric conductivity and electrocatalytic activity of graphene and PdNPs, as well as the host-guest recognition and enrichment ability of SH-β-CD. Under optimum conditions, differential pulse voltammetry (DPV) was used to measure the peak currents of the two drugs. The linear response ranges for rutin and isoquercitrin are 1.0×10-9-3.0×10-5molL-1and 5.0×10-12-5.0×10-6molL-1, with low detection limits of 3.0×10-10molL-1and 1.6×10-12molL-1, respectively. The proposed method might offer a new possibility for electrochemical analysis of rutin and isoquercitrin in pharmaceuticals or human serum owing to its low cost, simplicity, high sensitivity and good stability.
authors.affiliation: Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address: guoyj@sxu.edu.cn.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Guo
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010165:Palladium / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis; D012431:Rutin / Q000032:analysis*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D013438:Sulfhydryl Compounds; D047392:beta-Cyclodextrins; C016527:isoquercitrin; D012431:Rutin; D010165:Palladium; D006108:Graphite; D011794:Quercetin; C031215:betadex
keywords: Electrochemical detection; Graphene; Isoquercitrin; Palladium nanoparticles; Rutin; SH-β-Cyclodextrin