title: 3D composites based on the blends of chitosan and collagen with the addition of hyaluronic acid.
abstract: 3D porous composites based on blends of chitosan, collagen and hyaluronic acid were obtained through the lyophilization process. Mechanical properties, swelling behavior and thermal stability of the blends were studied. Moreover, SEM images were taken and the structure of the blends was studied. Biological properties of the materials obtained were investigated by analyzing of proliferation rate of fibroblast cells incubated with biomaterial extract using MTT assay (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide). The results showed that the properties of 3D composites based on the blends of chitosan and collagen were altered after the addition 1%, 2% and 5% of hyaluronic acid. Mechanical properties and thermal stability of chitosan/collagen blends were improved in the presence of hyaluronic acid in the composite. New 3D materials based on the blends of chitosan, collagen and hyaluronic acid were non-toxic and did not significantly affect cell morphology.
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, Gagarin 7, 87-100 Toruń, Poland. Electronic address: as@chem.umk.pl.
authors.forename: Alina
authors.initials: A
authors.lastname: Sionkowska
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, Gagarin 7, 87-100 Toruń, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Kaczmarek
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, Gagarin 7, 87-100 Toruń, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Lewandowska
authors.affiliation: Nicolaus Copernicus University in Torun, Faculty of Chemistry, Department of Chemistry of Biomaterials and Cosmetics, Gagarin 7, 87-100 Toruń, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Grabska
authors.affiliation: Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Faculty of Medicine, Chair of Urology, Department of Regenerative Medicine, Karlowicza 24 Street, 85-092 Bydgoszcz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Pokrywczyńska
authors.affiliation: Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Faculty of Medicine, Chair of Urology, Department of Regenerative Medicine, Karlowicza 24 Street, 85-092 Bydgoszcz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kloskowski
authors.affiliation: Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Faculty of Medicine, Chair of Urology, Department of Regenerative Medicine, Karlowicza 24 Street, 85-092 Bydgoszcz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Drewa
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D055595:Mechanical Phenomena; D016062:Porosity; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid; D003094:Collagen; D048271:Chitosan
keywords: Chitosan; Collagen; Composites; Hyaluronic acid; Mechanical properties