title: Berberine modulates cisplatin sensitivity of human gastric cancer cells by upregulation of miR-203.
abstract: Chemotherapeutic resistance is the main reason of the failure in clinical treatment of gastric cancer. Berberine (BER) is the active compound of traditional Chinese medicine Huang Lian. The aim of this present study is to evaluate the effect of BER on cisplatin resistance in gastric cancer cells and to investigate its possible mechanism. Gastric cancer cell lines SGC-7901 and BGC-823 and their respective cisplatin-resistant variants SGC-7901/DDP and BGC-823/DDP were used in this study. We found that BER treatment significantly reversed cisplatin sensitivity and induced caspase-dependent apoptosis in SGC-7901/DDP and BGC-823/DDP cells; BER treatment induced miR-203 expression, and overexpression of miR-203 mimicked the cisplatin-sensitizing effect of BER. Importantly, we showed that miR-203 was able to target the 3'UTR of Bcl-w. Therefore, we conclude that BER treatment reduces cisplatin resistance of gastric cancer cells by modulating the miR-203/Bcl-w apoptotic axis. BER may be a novel agent to enhance chemotherapeutic responses in cisplatin-resistant gastric cancer patients.
authors.affiliation: Department of Laparoscopic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. yheyi2016@126.com.
authors.forename: He-Yi
authors.initials: HY
authors.lastname: You
authors.affiliation: Department of Laparoscopic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Xue-Meng
authors.initials: XM
authors.lastname: Xie
authors.affiliation: Department of Laparoscopic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Laparoscopic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Heng-Liang
authors.initials: HL
authors.lastname: Zhu
authors.affiliation: Department of Laparoscopic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Fei-Zhao
authors.initials: FZ
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D001599:Berberine / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; C491073:BCL2L2 protein, human; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D001599:Berberine; D002945:Cisplatin
keywords: Apoptosis; Bcl-w; Berberine; Gastric cancer; MicroRNA-203