title: A label-free electrochemiluminescent sensor for ATP detection based on ATP-dependent ligation.
abstract: In this work, we describe a new label-free, sensitive and highly selective strategy for the electrochemiluminescent (ECL) detection of ATP at the picomolar level via ATP-induced ligation. The molecular-beacon like DNA probes (P12 complex) are self-assembled on a gold electrode. The presence of ATP leads to the ligation of P12 complex which blocks the digestion by Exonuclease III (Exo III). The protected P12 complex causes the intercalation of numerous ECL indicators (Ru(phen)3(2+)) into the duplex DNA grooves, resulting in significantly amplified ECL signal output. Since the ligating site of T4 DNA ligase and the nicking site of Exo III are the same, it involves no long time of incubation for conformation change. The proposed strategy combines the amplification power of enzyme and the inherent high sensitivity of the ECL technique and enables picomolar detection of ATP. The developed strategy also shows high selectivity against ATP analogs, which makes our new label-free and highly sensitive ligation-based method a useful addition to the amplified ATP detection arena.
authors.affiliation: Xiamen Huaxia University, Xiamen 361024, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China.
authors.forename: Chunshui
authors.initials: C
authors.lastname: Lin
authors.affiliation: Xiamen Huaxia University, Xiamen 361024, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China. Electronic address: xichen@xmu.edu.cn.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000255:Adenosine Triphosphate; D015374:Biosensing Techniques; D004247:DNA; D015342:DNA Probes; D004566:Electrodes; D008163:Luminescent Measurements
chemical_list: D015342:DNA Probes; D000255:Adenosine Triphosphate; D004247:DNA
keywords: ATP; Electrochemiluminescent (ECL); Exonuclease III; T4 DNA ligase