title: Erythrocyte deformability - A partner of the inflammatory response.
abstract: We aim to establish an in vivo animal model of acute inflammation using PAF (platelet activating factor) as inflammatory agent and to study the erythrocyte deformability changes induced by the inflammatory response. Counting the number of rolling and adherent neutrophils to endothelium after 2, 4 and 6h of intrascrotal injection of PAF we showed the induction of an inflammatory state. Blood samples are collected in order to measure the erythrocyte deformability and to quantify NO efflux from the red blood cells (RBCs). The results show an increased number of rolling and adherent neutrophils after 2h and 4h of inflammation as well as decreased values of erythrocyte deformability in the same time-points. This result is in line with the need of a low blood viscosity to the recruitment process that will improve leukocyte migration towards the endothelial wall. NO efflux from RBCs is also affected by the inflammatory response at the first hours of inflammation. This animal model demonstrates in vivo the association between an acute inflammatory response and the rheological properties of the blood, namely the RBCs deformability. For those reasons we consider this as an adequate model to study acute inflammatory responses as well as hemorheological parameters.
authors.affiliation: CSaldanha Lab, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Professor Egas Moniz, 1649-028 Lisbon, Portugal. Electronic address: anarmsilva@medicina.ulisboa.pt.
authors.forename: Ana Santos
authors.initials: AS
authors.lastname: Silva-Herdade
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Sicily, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Andolina
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Sicily, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Faggio
authors.affiliation: CSaldanha Lab, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Professor Egas Moniz, 1649-028 Lisbon, Portugal.
authors.forename: Ângelo
authors.initials: Â
authors.lastname: Calado
authors.affiliation: CSaldanha Lab, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av. Professor Egas Moniz, 1649-028 Lisbon, Portugal.
authors.forename: Carlota
authors.initials: C
authors.lastname: Saldanha
mesh_terms: D000009:Abdominal Muscles / Q000098:blood supply*; D000818:Animals; D001809:Blood Viscosity; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology*; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D000069416:Intravital Microscopy; D036904:Leukocyte Rolling; D008822:Mice, Transgenic; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D009569:Nitric Oxide / Q000097:blood; D010972:Platelet Activating Factor; D012039:Regional Blood Flow; D013314:Stress, Mechanical; D013997:Time Factors; D014699:Venules / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
chemical_list: D010972:Platelet Activating Factor; D009569:Nitric Oxide
keywords: Erythrocyte deformability; Inflammation; Intravital microscopy; Nitric oxide