title: Larsenimonas suaedae sp. nov., a moderately halophilic, endophytic bacterium isolated from the halophyte Suaeda salsa.
abstract: A moderately halophilic, Gram-stain-negative, non-endospore-forming endophytic bacterium designated strain ST307T was isolated from the euhalophyte Suaeda salsa in Dongying, China. Strain ST307T was aerobic, rod-shaped, motile and orange-yellow-pigmented. The organism grew at NaCl concentrations of 0.6-20 % (w/v) (optimum 5-6 %, w/v), at temperatures of 5-45 °C (optimum 35 °C) and at pH 5-9 (optimum pH 7-8). It accumulated poly-β-hydroxybutyric acid and produced exopolysaccharides. The major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. The predominant lipoquinone was ubiquinone Q-9. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a glycoaminolipid and a phosphoglycoaminolipid. The DNA G+C content was 60.5 mol%. Phylogenetic analyses of 16S rRNA gene sequences and concatenated atpA, rpoD and secA gene sequences revealed that the strain represents a member of the genus Larsenimonas. The closest related type strain was Larsenimonas salina M1-18T. Mean DNA-DNA relatedness values between strain ST307T and the related species L. salina M1-18T, Chromohalobacter beijerinckii DSM 7218T, C. canadensis DSM 6769T, C. israelensis DSM 6768T, C. marismortui CGMCC 1.2321T, C. nigrandesensis DSM 14323T, C. salexigens DSM 3043T and C. sarecensis DSM 15547T were 15±2-45±1 %. On the basis of phenotypic, chemotaxonomic and molecular features, strain ST307T clearly represents a novel species of the genus Larsenimonas. The name Larsenimonassuaedae sp. nov. is proposed, with ST307T (=CGMCC 1.8902T=DSM 22428T) as the type strain.
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Xia
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Hong-Zhen
authors.initials: HZ
authors.lastname: Wu
authors.affiliation: Medical College, Shandong Xiehe University, Jinan 250107, PR China.
authors.forename: Chun-Xiao
authors.initials: CX
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Guo-Yan
authors.initials: GY
authors.lastname: Zhao
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Wang
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, PR China.
authors.forename: Mei-Xue
authors.initials: MX
authors.lastname: Dai
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019663:Chenopodiaceae / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D044129:Halomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006885:Hydroxybutyrates / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D011091:Polyesters / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010743:Phospholipids; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C030571:ubiquinone 9