title: Influence of phenol on ammonia removal in an intermittent aeration bioreactor treating biologically pretreated coal gasification wastewater.
abstract: A laboratory-scale intermittent aeration bioreactor was investigated to treat biologically pretreated coal gasification wastewater that was mainly composed of NH3-N and phenol. The results showed that increasing phenol loading had an adverse effect on NH3-N removal; the concentration in effluent at phenol loading of 40mgphenol/(L·day) was 7.3mg/L, 36.3% of that at 200mg phenol/(L·day). The enzyme ammonia monooxygenase showed more sensitivity than hydroxylamine oxidoreductase to the inhibitory effect of phenol, with 32.2% and 10.5% activity inhibition, respectively at 200mg phenol/(L·day). Owing to intermittent aeration conditions, nitritation-type nitrification and simultaneous nitrification and denitrification (SND) were observed, giving a maximum SND efficiency of 30.5%. Additionally, ammonia oxidizing bacteria (AOB) and denitrifying bacteria were the main group identified by fluorescent in situ hybridization. However, their relative abundance represented opposite variations as phenol loading increased, ranging from 30.1% to 17.5% and 7.6% to 18.2% for AOB and denitrifying bacteria, respectively.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: han13946003379@163.com.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Han
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Shengyong
authors.initials: S
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D000641:Ammonia / Q000032:analysis* / Q000737:chemistry; D019149:Bioreactors / Q000382:microbiology*; D003031:Coal; D000066428:Coal Industry; D058440:Denitrification / Q000187:drug effects; D058465:Nitrification / Q000187:drug effects; D019800:Phenol / Q000633:toxicity*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000641:Ammonia; D003031:Coal; D019800:Phenol; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Coal gasification wastewater; Denitrification; Fluorescent in situ hybridization; Nitritation-type nitrification; Phenol