title: COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma.
abstract: The correlation between nuclear factor-kappa B (NF-κB) and COMMD7 in hepatocellular carcinoma (HCC) development remained unclear. Here, our clinicopathological data showed that COMMD7 is overexpressed in HCC with a correlation to NF-κB. Using HepG2 and SMMC-7721 cells that aberrantly overexpressed COMMD7, we found that NF-κB directly binds with COMMD7 promoter and serves as an activator for COMMD7 transcription by luciferase reporter assay, chromatin immunoprecipitation (ChIP), and electrophoretic mobility shift assay (EMSA). In both HepG2 cells and SMMC-7721 cells, the silencing of COMMD7 significantly inhibited the cell proliferation, whereas NF-κB silencing inhibited the expression of COMMD7 and further inhibited cell proliferation. In addition, cell apoptosis was promoted by COMMD7 silencing, and further promoted by NF-κB silencing. Cell migration and invasion were also inhibited by COMMD7 silencing, and further inhibited by NF-κB silencing. Thus, COMMD7 is correlated with a novel NF-κB positive feedback loop in hepatocellular carcinoma. Developing strategies for the treatment of HCC should consider the correlation between NF-κB and COMMD7, so as to improve the specificity and sensitivity of therapy and to reduce toxicity.
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Deng
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Huang
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Nan
authors.initials: N
authors.lastname: You
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Tang
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liang
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Na
authors.initials: N
authors.lastname: Mi
authors.affiliation: Department of Hepatobiliary Surgery, Xinqiao Hospital of Third Military Medical University, Chongqing, 400037, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D017209:Apoptosis; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D025461:Feedback, Physiological; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C554642:COMMD7 protein, human; D016328:NF-kappa B
keywords: COMMD7; apoptosis; hepatocellular carcinoma; nuclear factor-kappa B; proliferation