title: The mechanism of action of FXR1P-related miR-19b-3p in SH-SY5Y.
abstract: The biological effects of microRNAs (miRNAs) in the Fragile X Syndrome (FXS) have been widely studied. Dysregulation of miRNAs plays a critical role in the progression of nervous system diseases and in cell proliferation and differentiation. Our previous study validated that miR-19b-3p was associated with FXR1 (Fragile X related gene 1), one of homologous genes of FMR1 (Fragile X mental retardation 1). The purpose of this study was to investigate the relationship of FXR1 and miR-19b-3p, and the crucial role of miR-19b-3p in FXS and to validate whether miR-19b-3p could regulate the growth of SH-SY5Y cells. We determined that miR-19b-3p could regulate the expression of not only USP32, RAB18 and Dusp6 but also FXR1, and FXR1 could in turn regulate the expression of miR-19b-3p. What's more, the overexpression of miR-19b-3p significantly inhibited the proliferation, contributed the apoptosis and slowed down the cycle of SH-SY5Y cells. Taken together, our results indicate that miR-19b-3p plays a significant role in the molecular pathology of FXS by interacting with FXR1 and influencing the growth of SH-SY5Y cells.
authors.affiliation: Department of Biochemistry & Biology, University of South China, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China. Electronic address: luckymayun@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biochemistry & Biology, University of South China, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China. Electronic address: tianshuaismile@163.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Tian
authors.affiliation: Department of Biochemistry & Biology, University of South China, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China. Electronic address: heshuya8502@163.com.
authors.forename: Shuya
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Geriatric Medicine, Department of Respiratory, Xiangya Hospital of Central South University, Changsha 410008, PR China. Electronic address: chenqiong12@hotmail.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China. Electronic address: wangzb65@hotmail.com.
authors.forename: Zongbao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Biochemistry & Biology, University of South China, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Biochemistry & Biology, University of South China, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Fu
authors.affiliation: Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, 28 Western Changsheng Road, Hengyang City, Hunan Province 421001, PR China. Electronic address: 1622214323@qq.com.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Lei
mesh_terms: D017209:Apoptosis; D001321:Autistic Disorder / Q000235:genetics; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D054642:Dual Specificity Phosphatase 6 / Q000235:genetics; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D043222:Ubiquitin Thiolesterase / Q000235:genetics; D020691:rab GTP-Binding Proteins / Q000235:genetics
chemical_list: C096916:FXR1 protein, human; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; C484168:RAB18 protein, human; D016601:RNA-Binding Proteins; C431363:USP10 protein, human; D054642:Dual Specificity Phosphatase 6; D043222:Ubiquitin Thiolesterase; D020691:rab GTP-Binding Proteins
keywords: Dusp6; FXR1; RAB18; USP32; miR-19b