title: Investigation of juglone effects on metastasis and angiogenesis in pancreatic cancer cells.
abstract: Juglone, a natural component, is shown to have cytotoxic and apoptotic effects in several cancer cell lines. However, little is known about its effects on invasion and metastasis. In this study, we aimed to determine the antimetastatic effect of juglone in the BxPC-3 and PANC-1 pancreatic cancer cell lines. Cytotoxic effect of juglone was evaluated by using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) test. The cells were treated with juglone at <IC50 doses (5, 10, 15 and 20μM ) for 24h. After the cell adhesion and invasion analysis, expression profiles of the MMP-2, MMP-9 and Phactr-1 genes were determined by qPCR. The IC50 dose of juglone was found to be 21.05μM in the BxPC-3 cell line and 21.25μM in the PANC-1 cell line for 24h. According to the cell adhesion and invasion analysis, treatment of juglone for 24h reduced the adhesion and invasion features of pancreatic cancer cells. A significant reduction of MMP-2, MMP-9 and Phactr-1 expressions was observed in pancreatic cancer cells after the treatment of juglone at <IC50 doses. By this study, it has been shown for the first time that juglone inhibits cell invasion and metastasis in pancreatic cancer line and can be evaluated as an effective anticancer agent in pancreatic cancer.
authors.affiliation: Necmettin Erbakan University Faculty of Meram Medicine, Department of Medical Biology, Konya, Turkey. Electronic address: ebruavc.87@gmail.com.
authors.forename: Ebru
authors.initials: E
authors.lastname: Avcı
authors.affiliation: Selcuk University Faculty of Medicine, Department of Medical Biology, Konya, Turkey.
authors.forename: Hilal
authors.initials: H
authors.lastname: Arıkoğlu
authors.affiliation: Selcuk University Faculty of Medicine, Department of Medical Biology, Konya, Turkey.
authors.forename: Dudu
authors.initials: D
authors.lastname: Erkoç Kaya
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics; D009285:Naphthoquinones / Q000494:pharmacology*; D009362:Neoplasm Metastasis / Q000188:drug therapy*; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D009285:Naphthoquinones; D008666:Metalloendopeptidases; C005134:juglone
keywords: Juglone; Metastasis; Pancreatic cancer cell lines