title: In vitro effect of imatinib mesylate loaded on polybutylcyanoacrylate nanoparticles on leukemia cell line K562.
abstract: The study aimed to prepare imatinib mesylate-loaded polybutylcyanoacrylate (PBCA) nanoparticles and evaluate their efficacy on leukemia cell line K562. The formulation was prepared by miniemulsion polymerization technique. Nanoparticles were characterized by dynamic light scattering (DLS), spectrophotometry, Fourier transform infrared spectroscopy (FTIR), dialysis membrane, and 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) techniques. Nanoscale particles with high encapsulation efficiency (86%) and physical entrapment of drug were observed. In addition, nanoparticles showed suitable drug retention capability and potentiate the cytotoxicity effects of imatinib mesylate. Findings of study suggested PBCA nanoparticles are promising carrier for imatinib mesylate delivery to leukemia cell line K562.
authors.affiliation: a Department of Chemical Engineering, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Leyla
authors.initials: L
authors.lastname: Hasandoost
authors.affiliation: b Department of Pilot Nanobiotechnology , Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Azim
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: a Department of Chemical Engineering, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Attar
authors.affiliation: a Department of Chemical Engineering, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Heydarinasab
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D004655:Emulsions; D004659:Enbucrilate / Q000737:chemistry*; D006801:Humans; D000068877:Imatinib Mesylate / Q000737:chemistry / Q000494:pharmacology*; D020014:K562 Cells; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004655:Emulsions; D000068877:Imatinib Mesylate; D004659:Enbucrilate
keywords: FTIR; MTT; imatinib mesylate; leukemia; miniemulsion polymerization; poly butylcyanoacrylate nanoparticles