title: Analysis of esophageal cancer cell lines exposed to X-ray based on radiosensitivity influence by tumor necrosis factor-α.
abstract: Assess the effects of tumor necrosis factor-α (TNF-α) in enhancing the radiosensitivity of esophageal cancer cell line in vitro. Three esophageal cancer cell line cells were exposed to X-ray with or without TNF-α treatment. MTT assay was used to evaluate the cell growth curve, and flow cytometry was performed to assess the cell apoptosis. The radiosensitizing effects of TNF-α were detected by cell colony formation assay. Western blotting was applied to observe the expression of NF-κB and caspase-3 protein in the exposed cells. Our results indicated that cellular inhibition rate increased over time, the strongest is combined group (P < 0.05). Western blotting showed that the decline expression of NF-κB protein was stated between only rhTNF-α and only X-ray radiation group and the maximum degree was manifested in combined group. Caspase-3 protein content expression just works opposite. Three kinds of cells in the NF-κB protein were similar without rhTNF-α. Then SEG1 NF-κB protein content was reduced more than other two kinds. We concluded that the cells treated with TNF-α showed significantly suppressed cell proliferation, increasing the cell apoptosis, and caspase-3 protein expression after X-ray exposure. TNF-α can enhance the radiosensitivity of esophageal cancer to enhancing the effect of the former.
authors.affiliation: Department of Oncology of Northern Jiangsu People's Hospital, Yangzhou, Jiangsu Province, China.
authors.forename: Buhai
authors.initials: B
authors.lastname: Wang
authors.affiliation: Research Center of Cancer Prevention and Treatment, Medical College of Yangzhou University.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Research Center of Cancer Prevention and Treatment, Medical College of Yangzhou University.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008954:Models, Biological; D011836:Radiation Tolerance / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay; D014965:X-Rays
chemical_list: D000970:Antineoplastic Agents; D014409:Tumor Necrosis Factor-alpha
keywords: Tumor necrosis factor-α; esophageal cancer; radiation-sensitizing agents; rhTNF-α