title: Induced Pluripotent Stem Cells Meet Genome Editing.
abstract: It is extremely rare for a single experiment to be so impactful and timely that it shapes and forecasts the experiments of the next decade. Here, we review how two such experiments-the generation of human induced pluripotent stem cells (iPSCs) and the development of CRISPR/Cas9 technology-have fundamentally reshaped our approach to biomedical research, stem cell biology, and human genetics. We will also highlight the previous knowledge that iPSC and CRISPR/Cas9 technologies were built on as this groundwork demonstrated the need for solutions and the benefits that these technologies provided and set the stage for their success.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: hockemeyer@berkeley.edu.
authors.forename: Dirk
authors.initials: D
authors.lastname: Hockemeyer
authors.affiliation: The Whitehead Institute for Biomedical Research and Department of Biology, MIT, Cambridge, MA 02142, USA.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jaenisch
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics; D065150:Cellular Reprogramming / Q000235:genetics; D044127:Epigenesis, Genetic; D000072669:Gene Editing; D015894:Genome, Human; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*