title: Antiprotozoal activity of major constituents from the bioactive fraction of Verbesina encelioides.
abstract: The bioactive petroleum ether fraction of Verbesina encelioides, previously studied by the authors, was chosen for the isolation of antiprotozoal metabolites. Pseudotaraxasterol-3β-acetate (1), benzyl 2,6-dimethoxy benzoate (2), 16β-hydroxy-pseudotaraxasterol-3β-palmitate (3) and pseudotaraxasterol (4), in addition to β-sitosterol glucoside (5) and β-sitosterol galactoside (6) were isolated and identified based on one-dimensional and two-dimensional spectral analysis. This is the first report describing (3) and (6) in genus Verbesina. The isolated compounds were tested in vitro against Plasmodium falciparum, Trypanosoma brucei, Trypanosoma cruzi and Leishmania infantum. Cytotoxicity was evaluated on MRC-5 cells. Compound 1 showed moderate to weak activity against L. infantum T. brucei and P. falciparum and was inactive against T. cruzi. Compound 3 showed moderate activity against L. infantum, compound 4 revealed weak activity against T. cruzi, while 5 and 6 were inactive against all tested protozoa. All compounds were non-cytotoxic. The isolated constituents showed less antiprotozoal activity than the crude fraction.
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Cairo University , Cairo , Egypt.
authors.forename: Shahira M
authors.initials: SM
authors.lastname: Ezzat
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Cairo University , Cairo , Egypt.
authors.forename: Maha M
authors.initials: MM
authors.lastname: Salama
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Cairo University , Cairo , Egypt.
authors.forename: Engy A
authors.initials: EA
authors.lastname: Mahrous
authors.affiliation: b Laboratory for Microbiology, Parasitology and Hygiene (LMPH) , Antwerp University , Antwerp , Belgium.
authors.forename: Louis
authors.initials: L
authors.lastname: Maes
authors.affiliation: c Functional Food Center , Korea Institute of Science and Technology , Gangneung , Republic of Korea.
authors.forename: Cheol-Ho
authors.initials: CH
authors.lastname: Pan
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Cairo University , Cairo , Egypt.
authors.forename: Essam
authors.initials: E
authors.lastname: Abdel-Sattar
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D018314:Leishmania infantum / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects; D014344:Trypanocidal Agents / Q000494:pharmacology; D014346:Trypanosoma brucei brucei / Q000187:drug effects; D014349:Trypanosoma cruzi / Q000187:drug effects; D031208:Verbesina / Q000737:chemistry*
chemical_list: D000962:Antimalarials; D000981:Antiprotozoal Agents; D010936:Plant Extracts; D014344:Trypanocidal Agents
keywords: Antimalarial; antileishmanial; antitrypanosomal; cytotoxicity