title: Kaurane and abietane diterpenoids from the roots of Tripterygium wilfordii and their cytotoxic evaluation.
abstract: Kaurane diterpenoids and abietane diterpenoids are the major anti-tumor components in Tripterygium wilfordii (Celastraceae). Extraction of Tripterygium wilfordii afforded a new diterpenoid and twenty known diterpenoids belonging to the abietane and ent-kaurane families. The structures of all these compounds were characterized by spectroscopic techniques. All compounds were evaluated using the MTT method for in vitro cytotoxicity against six human cancer cell lines (HepG2, Hep3B, Bcap37, U251, MCF-7 and A549). Compounds 7 and 12 exhibited marked cytotoxicity against a six cell lines with IC50 values in the range 5.10-23.30μM. The structure-cytotoxic activity relationships of these diterpenoids are also discussed.
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 11016, PR China; Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Gao
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 11016, PR China; Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 11016, PR China; Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, PR China. Electronic address: xiaoxiao270@163.com.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Huang
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 11016, PR China; Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, PR China. Electronic address: songsj99@163.com.
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Song
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry*; D000091402:Prohibitins; D013329:Structure-Activity Relationship; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane; C000717331:PHB2 protein, human; D000091402:Prohibitins
keywords: Aromatic abietane; Celastraceae; Cytotoxicity; Diterpenoids; Ent-kaurane; Tripterygium wilfordii