title: Guidelines for May-Grünwald-Giemsa staining in haematology and non-gynaecological cytopathology: recommendations of the French Society of Clinical Cytology (SFCC) and of the French Association for Quality Assurance in Anatomic and Cytologic Pathology (AFAQAP).
abstract: OBJECTIVE
  Since the guidelines of the International Committee for Standardisation in Haematology (ICSH) in 1984 and those of the European Committee for External Quality Assessment Programmes in Laboratory Medicine (EQALM) in 2004, no leading organisation has published technical recommendations for the preparation of air-dried cytological specimens using May-Grünwald-Giemsa (MGG) staining.
  
  
  METHODS
  Literature data were retrieved using reference books, baseline-published studies, articles extracted from PubMed/Medline and Google Scholar, and online-available industry datasheets.
  
  
  BACKGROUND
  The present review addresses all pre-analytical issues concerning the use of Romanowsky's stains (including MGG) in haematology and non-gynaecological cytopathology. It aims at serving as actualised, best practice recommendations for the proper handling of air-dried cytological specimens. It, therefore, appears complementary to the staining criteria of the non-gynaecological diagnostic cytology handbook edited by the United Kingdom National External Quality Assessment Service (UK-NEQAS) in February 2015.
authors.affiliation: Centre de Pathologie Est, Hospices Civils de Lyon, Université Claude Bernard Lyon 1, Bron, France. eric.piaton@chu-lyon.fr.|Société Française de Cytologie Clinique (SFCC), Paris, France. eric.piaton@chu-lyon.fr.
authors.forename: E
authors.initials: E
authors.lastname: Piaton
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Service d'Anatomie et Cytologie Pathologiques, Hôpital Universitaire Necker Enfants Malades, Université Paris Descartes, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Fabre
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Laboratoire d'Anatomo-Pathologie, Centre Hospitalier René Dubos, Cergy Pontoise, France.
authors.forename: I
authors.initials: I
authors.lastname: Goubin-Versini
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Cabinet de Pathologie, Strasbourg, France.
authors.forename: M-F
authors.initials: MF
authors.lastname: Bretz-Grenier
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Département d'Anatomie et Cytologie Pathologiques, IUC Toulouse Oncopôle, Toulouse, France.
authors.forename: M
authors.initials: M
authors.lastname: Courtade-Saïdi
authors.affiliation: Unité de Cytopathologie, Gustave Roussy, Villejuif, France.
authors.forename: S
authors.initials: S
authors.lastname: Vincent
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Service d'Anatomie et Cytologie Pathologiques, Hôpital Haut-Lévêque (CHU de Bordeaux), Pessac, France.
authors.forename: G
authors.initials: G
authors.lastname: Belleannée
authors.affiliation: Centre de Pathologie Est, Hospices Civils de Lyon, Université Claude Bernard Lyon 1, Bron, France.|Société Française de Cytologie Clinique (SFCC), Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Thivolet
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|UF de Cytologie Pathologique, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: J
authors.initials: J
authors.lastname: Boutonnat
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Cabinet de Pathologie, SCP des Drs Bloget et Declerck, Avon, France.
authors.forename: H
authors.initials: H
authors.lastname: Debaque
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Service d'Anatomie et Cytologie Pathologiques, Hôpital Tenon, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Fleury-Feith
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Unité de Cytopathologie, Gustave Roussy, Villejuif, France.
authors.forename: P
authors.initials: P
authors.lastname: Vielh
authors.affiliation: Association Française d'Assurance Qualité en Anatomie et Cytologie Pathologiques (AFAQAP), Strasbourg, France.|Département de Pathologie, Hôpital de Hautepierre, CHU de Strasbourg, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Egelé
authors.affiliation: Association Française d'Assurance Qualité en Anatomie et Cytologie Pathologiques (AFAQAP), Strasbourg, France.|Département de Pathologie, Hôpital de Hautepierre, CHU de Strasbourg, Strasbourg, France.
authors.forename: J-P
authors.initials: JP
authors.lastname: Bellocq
authors.affiliation: Association Française d'Assurance Qualité en Anatomie et Cytologie Pathologiques (AFAQAP), Strasbourg, France.|Service d'Anatomie et Cytologie Pathologiques, Hôpital Pasteur, CHU de Nice, Nice, France.
authors.forename: J-F
authors.initials: JF
authors.lastname: Michiels
authors.affiliation: Société Française de Cytologie Clinique (SFCC), Paris, France.|Association Française d'Assurance Qualité en Anatomie et Cytologie Pathologiques (AFAQAP), Strasbourg, France.|Service d'Anatomie et Cytologie Pathologiques, Université Paris Descartes, Hôpitaux Universitaires Paris Centre, site Cochin, AP-HP, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Cochand-Priollet
mesh_terms: D003581:Cytodiagnosis; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005602:France; D017408:Guidelines as Topic; D006405:Hematology / Q000379:methods* / Q000592:standards; D006801:Humans; D008751:Methylene Blue / Q000737:chemistry; D011785:Quality Assurance, Health Care; D013194:Staining and Labeling; D006113:United Kingdom
chemical_list: C043260:May-Grunwald Giemsa; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)
keywords: Giemsa; May-Grünwald; Romanowsky's stains; Wright; non-gynaecological cytopathology; quality assurance