title: Imaging Approaches to Investigate Myonuclear Positioning in Drosophila.
abstract: In the skeletal muscle, nuclei are positioned at the periphery of each myofiber and are evenly distributed along its length. Improper positioning of myonuclei has been correlated with muscle disease and decreased muscle function. Several mechanisms required for regulating nuclear position have been identified using the fruit fly, Drosophila melanogaster. The conservation of the myofiber between the fly and vertebrates, the availability of advanced genetic tools, and the ability to visualize dynamic processes using fluorescent proteins in vivo makes the fly an excellent system to study myonuclear positioning. This chapter describes time-lapse and fixed imaging methodologies using both the Drosophila embryo and the larva to investigate mechanisms of myonuclear positioning.
authors.affiliation: Graduate Program in Basic and Applied Biology (GABBA), Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal.|Program in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA.
authors.forename: Mafalda
authors.initials: M
authors.lastname: Azevedo
authors.affiliation: Program in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA.|Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520, USA.
authors.forename: Victoria K
authors.initials: VK
authors.lastname: Schulman
authors.affiliation: Program in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA.|Biology Department, Boston College, 140 Commonwealth Avenue, Chestnut Hill, MA, 02467, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Folker
authors.affiliation: Program in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA.|Weill Graduate School at Cornell Medical College, 430 East 67th Street, New York, NY, 10021, USA.
authors.forename: Mridula
authors.initials: M
authors.lastname: Balakrishnan
authors.affiliation: Program in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA. m-baylies@ski.mskcc.org.|Weill Graduate School at Cornell Medical College, 430 East 67th Street, New York, NY, 10021, USA. m-baylies@ski.mskcc.org.
authors.forename: Mary
authors.initials: M
authors.lastname: Baylies
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004330:Drosophila; D004625:Embryo, Nonmammalian; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000379:methods; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D059008:Time-Lapse Imaging
keywords: Drosophila; Embryo; Larvae; Muscle; Nuclear movement