title: Regional analysis of potential polychlorinated biphenyl degrading bacterial strains from China.
abstract: Polychlorinated biphenyls (PCBs), the chlorinated derivatives of biphenyl, are one of the most prevalent, highly toxic and persistent groups of contaminants in the environment. The objective of this study was to investigate the biodegradation of PCBs in northeastern (Heilongjiang Province), northern (Shanxi Province) and eastern China (Shanghai municipality). From these areas, nine soil samples were screened for PCB-degrading bacteria using a functional complementarity method. The genomic 16S rDNA locus was amplified and the products were sequenced to identify the bacterial genera. Seven Pseudomonas strains were selected to compare the capacity of bacteria from different regions to degrade biphenyl by HPLC. Compared to the biphenyl content in controls of 100%, the biphenyl content went down to 3.7% for strain P9-324, 36.3% for P2-11, and 20.0% for the other five strains. These results indicate that a longer processing time led to more degradation of biphenyl. PCB-degrading bacterial strains are distributed differently in different regions of China.
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Shuai
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.
authors.forename: Xurun
authors.initials: X
authors.lastname: Yu
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
authors.forename: Ai-Sheng
authors.initials: AS
authors.lastname: Xiong
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China. Electronic address: feixiong@yzu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiong
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D004785:Environmental Pollutants / Q000378:metabolism*; D010802:Phylogeny; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D004785:Environmental Pollutants; D012336:RNA, Ribosomal, 16S; D012987:Soil; D011078:Polychlorinated Biphenyls
keywords: Biodegradation; Biphenyl; HPLC; Polychlorinated biphenyls; Pseudomonas sp.