title: Prevalence of Antibodies against Neospora caninum in Père David's Deer ( Elaphurus davidianus ) in Beijing, China.
abstract: Neospora caninum is an apicomplexan protozoan and causes neuromuscular disorders in canids and abortions in cattle worldwide. We screened sera samples from 49 free-ranging Père David's deer ( Elaphurus davidianus ) in a nature reserve in Beijing, China, for antibodies against N. caninum using indirect fluorescence antibody tests and western blot tests. Antibodies were found in 27% of the deer. Western blot analysis revealed antibody reactivity against immunodominant N. caninum antigens of 16, 25, and 37 kDa in size together with other visible bands.
authors.affiliation: 1 Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China;|2 National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China;
authors.forename: Fangjie
authors.initials: F
authors.lastname: Han
authors.affiliation: 1 Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China;|2 National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China;
authors.forename: Yong
authors.initials: Y
authors.lastname: Fu
authors.affiliation: 3 Beijing Museum of Natural History, Beijing, 100050, China;
authors.forename: Changsheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: 1 Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China;|2 National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China;
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: 1 Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China;|2 National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China;
authors.forename: Jianhai
authors.initials: J
authors.lastname: Xu
authors.affiliation: 1 Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China;|2 National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China;|4 State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China;
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D000068476:Beijing / Q000453:epidemiology; D003048:Coccidiosis / Q000097:blood / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D003670:Deer / Q000097:blood*; D016036:Seroepidemiologic Studies
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan
keywords: Antibody prevalence; China; Neospora caninum; Père David's deer