title: A fully battery-powered inexpensive spectrophotometric system for high-sensitivity point-of-care analysis on a microfluidic chip.
abstract: A cost-effective b[combining low line]a[combining low line]ttery-powered s[combining low line]pectrophotometric s[combining low line]ystem (BASS) was developed for quantitative point-of-care (POC) analysis on a microfluidic chip. By using methylene blue as a model analyte, we first compared the performance of the BASS with a commercial spectrophotometric system, and further applied the BASS for loop-mediated isothermal amplification (LAMP) detection and subsequent quantitative nucleic acid analysis which exhibited a comparable limit of detection to that of Nanodrop. Compared to the commercial spectrophotometric system, our spectrophotometric system is lower-cost, consumes less reagents, and has higher detection sensitivity. Most importantly, it does not rely on external power supplies. All these features make our spectrophotometric system highly suitable for a variety of POC analyses, such as field detection.
authors.affiliation: Department of Chemistry, University of Texas at El Paso, 500 West University Ave, El Paso, Texas 79968, USA.
authors.forename: Maowei
authors.initials: M
authors.lastname: Dou
authors.forename: Juan
authors.initials: J
authors.lastname: Lopez
authors.forename: Misael
authors.initials: M
authors.lastname: Rios
authors.forename: Oscar
authors.initials: O
authors.lastname: Garcia
authors.forename: Chuan
authors.initials: C
authors.lastname: Xiao
authors.forename: Michael
authors.initials: M
authors.lastname: Eastman
authors.forename: XiuJun
authors.initials: X
authors.lastname: Li