title: Intestinal Expression of Genes Encoding Inflammatory Mediators and Gelatinases During Arcobacter Butzleri Infection of Gnotobiotic Il-10 Deficient Mice.
abstract: We have previously shown that Arcobacter butzleri induces intestinal, extra-intestinal, and systemic immune responses in perorally infected gnotobiotic IL-10(-/-) mice in a strain-dependent fashion. Here, we present a comprehensive survey of small and large intestinal expression profiles of inflammatory and regulatory mediators as well as of the matrix-degrading gelatinases MMP-2 and MMP-9 following murine A. butzleri infection. Gnotobiotic IL-10(-/-) mice were infected with A. butzleri strains CCUG 30485 or C1 of human and chicken origin, respectively. At day 6 following A. butzleri infection, mucin-2 mRNA, an integral part of the intestinal mucus layer, was downregulated in the colon, whereas TNF and IL-23p19 mRNA were upregulated in the ileum. Furthermore, IFN-γ, IL-17A, IL-1β, and IL-22 mRNA were upregulated in both colonic and ileal ex vivo biopsies at day 6 post strain CCUG 30485 infection. These changes were accompanied by downregulated colonic MMP-9 levels, whereas both MMP-2 and MMP-9 mRNA were upregulated in the ileum. In conclusion, these data indicate that A. butzleri infection induces changes in the expression of genes involved in pro-inflammatory and regulatory immune responses as well as in tissue degradation.
authors.affiliation: Department of Microbiology and Hygiene, Charité - University Medicine Berlin , Berlin, Germany.
authors.forename: Markus M
authors.initials: MM
authors.lastname: Heimesaat
authors.affiliation: Institute of Food Hygiene, Free University Berlin , Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Alter
authors.affiliation: Department of Microbiology and Hygiene, Charité - University Medicine Berlin , Berlin, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Bereswill
authors.affiliation: Institute of Food Hygiene, Free University Berlin , Berlin, Germany.
authors.forename: Greta
authors.initials: G
authors.lastname: Gölz
keywords: Arcobacter butzleri; Campylobacterales; IL-22/IL-18 axis; IL-23/Th17 axis; gelatinases; gnotobiotic IL-10–/– mouse infection model; host defence; intestinal tract; mucosal immunology; pro-inflammatory immune responses