title: Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments.
abstract: Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenhouse. Mercury chloride and Plant Preservative Mixture™ were used in the surface sterilizing stage, better results being achieved with Plant Preservative Mixture™ incorporation in culture medium, at any concentration. Polyvinylpyrrolidone, activated charcoal, cysteine, ascorbic acid or citric acid were added to the culture medium to avoid oxidation. After 30 days of culture, polyvinylpirrolidone and ascorbic acid gave better results, eliminating oxidation in most explants. For shoot multiplication, benzylaminopurine was used in concentrations of 4.4 and 8.8 µM in Woody Plant Medium, resulting in an average of 4.43 and 4.68 shoots per explant, respectively, after 90 days. Indole-3-butyric acid and α-naphthalene acetic acid were used to induce root formation, reaching a maximum rooting rate of 24% with 20µM α-naphthalene acetic acid. For acclimatization. the rooted plants were transferred to Plantmax® substrate and cultured in a greenhouse, reaching 79% of survival after 30 days and 60% after one year.
authors.affiliation: Departamento de Botânica, Universidade Federal do Paraná, Universidade Federal do Paraná, PR , Brazil, Programa de Pós-graduação em Agronomia (Produção Vegetal), Laboratório de Micropropagação, Departamento de Botânica, Universidade Federal do Paraná - UFPR, Av. Coronel Francisco Heráclito dos Santos, s/n, Jardim das Américas, CEP 81530-900, Curitiba, PR, Brazil.
authors.forename: S S
authors.initials: SS
authors.lastname: Silveira
authors.affiliation: Departamento de Botânica, Universidade Federal do Paraná, Universidade Federal do Paraná, PR , Brazil, Programa de Pós-graduação em Botânica, Laboratório de Micropropagação, Departamento de Botânica, Universidade Federal do Paraná - UFPR, Av. Coronel Francisco Heráclito dos Santos, s/n, Jardim das Américas, CP 19031, CEP 81531-980, Curitiba, PR, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Cordeiro-Silva
authors.affiliation: Embrapa Florestas, Embrapa Florestas, PR , Brazil, Embrapa Florestas, Estrada da Ribeira, Km 111, CEP 83411-000, Colombo, PR, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Degenhardt-Goldbach
authors.affiliation: Departamento de Botânica, Universidade Federal do Paraná, Universidade Federal do Paraná, PR , Brazil, Programa de Pós-graduação em Botânica, Laboratório de Micropropagação, Departamento de Botânica, Universidade Federal do Paraná - UFPR, Av. Coronel Francisco Heráclito dos Santos, s/n, Jardim das Américas, CP 19031, CEP 81531-980, Curitiba, PR, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Quoirin
mesh_terms: D029759:Calophyllum / Q000254:growth & development*; D003470:Culture Media; D018517:Plant Roots / Q000254:growth & development*; D018520:Plant Shoots / Q000254:growth & development*; D012639:Seeds / Q000254:growth & development*
chemical_list: D003470:Culture Media