title: 5-HT1A receptor gene silencers Freud-1 and Freud-2 are differently expressed in the brain of rats with genetically determined high level of fear-induced aggression or its absence.
abstract: Serotonin 5-HT1A receptor is known to play a crucial role in the mechanisms of genetically defined aggression. In its turn, 5-HT1A receptor functional state is under control of multiple factors. Among others, transcriptional factors Freud-1 and Freud-2 are known to be involved in the repression of 5-HT1A receptor gene expression. However, implication of these factors in the regulation of behavior is unclear. Here, we investigated the expression of 5-HT1A receptor and silencers Freud-1 and Freud-2 in the brain of rats selectively bred for 85 generations for either high level of fear-induced aggression or its absence. It was shown that Freud-1 and Freud-2 levels were different in aggressive and nonaggressive animals. Freud-1 protein level was decreased in the hippocampus, whereas Freud-2 protein level was increased in the frontal cortex of highly aggressive rats. There no differences in 5-HT1A receptor gene expression were found in the brains of highly aggressive and nonaggressive rats. However, 5-HT1A receptor protein level was decreased in the midbrain and increased in the hippocampus of highly aggressive rats. These data showed the involvement of Freud-1 and Freud-2 in the regulation of genetically defined fear-induced aggression. However, these silencers do not affect transcription of the 5-HT1A receptor gene in the investigated rats. Our data indicate the implication of posttranscriptional rather than transcriptional regulation of 5-HT1A receptor functional state in the mechanisms of genetically determined aggressive behavior. On the other hand, the implication of other transcriptional regulators for 5-HT1A receptor gene in the mechanisms of genetically defined aggression could be suggested.
authors.affiliation: Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Kondaurova
authors.affiliation: Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Ilchibaeva
authors.affiliation: Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia.
authors.forename: Anton S
authors.initials: AS
authors.lastname: Tsybko
authors.affiliation: Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia.
authors.forename: Rimma V
authors.initials: RV
authors.lastname: Kozhemyakina
authors.affiliation: Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia.
authors.forename: Nina K
authors.initials: NK
authors.lastname: Popova
authors.affiliation: Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, Russia. Electronic address: naumenko2002@bionet.nsc.ru.
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Naumenko
mesh_terms: D000374:Aggression / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D005239:Fear / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D013045:Species Specificity
chemical_list: C476534:Cc2d1a protein, rat; C000625144:Cc2d1b protein, rat; D012333:RNA, Messenger; D012097:Repressor Proteins; D044282:Receptor, Serotonin, 5-HT1A
keywords: 5-HT(1A) receptor; 5-HT(1A) receptor gene silencers Freud-1 and Freud-2; Aggression; Gene expression; Protein level; Rats