title: STAT5 Activation in the Dermal Papilla Is Important for Hair Follicle Growth Phase Induction.
abstract: Hair follicles are skin appendages that undergo periods of growth (anagen), regression (catagen), and rest (telogen) regulated by their mesenchymal component, the dermal papilla (DP). On the basis of the reports of its specific expression in the DP, we investigated signal transducer and activator of transcription (STAT5) activation during hair development and cycling. STAT5 activation in the DP began in late catagen, reaching a peak in early anagen before disappearing for the rest of the cycle. This was confirmed by the expression profile of suppressor of cytokine signaling 2, a STAT5 target in the DP. This pattern of expression starts after the first postnatal hair cycle. Quantification of hair cycling using the Flash canonical Wnt signaling in vivo bioluminescence reporter found that conditional knockout of STAT5A/B in the DP targeted through Cre-recombinase under the control of the Sox18 promoter resulted in delayed anagen entry compared with control. Microarray analysis of STAT5 deletion versus control revealed key changes in tumor necrosis factor-α, Wnt, and fibroblast growth factor ligands, known for their role in inducing anagen entry. We conclude that STAT5 activation acts as a mesenchymal switch to trigger natural anagen entry in postdevelopmental hair follicle cycling.
authors.affiliation: UQ Centre for Clinical Research, The University of Queensland, Herston, Queensland, Australia.
authors.forename: Julien M D
authors.initials: JMD
authors.lastname: Legrand
authors.affiliation: UQ Centre for Clinical Research, The University of Queensland, Herston, Queensland, Australia; UQ Diamantina Institute, The University of Queensland, Woolloongabba, Queensland, Australia.
authors.forename: Edwige
authors.initials: E
authors.lastname: Roy
authors.affiliation: UQ Diamantina Institute, The University of Queensland, Woolloongabba, Queensland, Australia.
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Ellis
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia.
authors.forename: Mathias
authors.initials: M
authors.lastname: Francois
authors.affiliation: UQ Diamantina Institute, The University of Queensland, Woolloongabba, Queensland, Australia; Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Brooks
authors.affiliation: UQ Centre for Clinical Research, The University of Queensland, Herston, Queensland, Australia; UQ Diamantina Institute, The University of Queensland, Woolloongabba, Queensland, Australia. Electronic address: k.khosrotehrani@uq.edu.au.
authors.forename: Kiarash
authors.initials: K
authors.lastname: Khosrotehrani
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000235:genetics*; D018859:Hair Follicle / Q000166:cytology / Q000254:growth & development*; D006801:Humans; D008163:Luminescent Measurements; D051379:Mice; D018345:Mice, Knockout; D046228:Microarray Analysis; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D050799:STAT5 Transcription Factor / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D050799:STAT5 Transcription Factor