title: The role of transketolase and octulose in the resurrection plant Craterostigma plantagineum.
abstract: Phylogenetic analysis revealed that Craterostigma plantagineum has two transketolase genes (transketolase 7 and 10) which are separated from the other transketolase genes including transketolase 3 from C. plantagineum We obtained recombinant transketolase 3, 7, and 10 of C. plantagineum and showed that transketolase 7 and 10 of C. plantagineum, but not transketolase 3, catalyse the formation of octulose-8-phosphate in vitro Transketolase 7 and 10 of C. plantagineum performed the exchange reaction that produces octulose-8-phosphate using glucose-6-phosphate and fructose-6-phosphate as substrates. Octulose is localized in the cytosol and phloem exudate analysis showed that octulose was the dominant sugar exported from the leaves to the roots.
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, 53115 Bonn, Germany.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Institute of Cellular and Molecular Botany, Department of Ecophysiology, University of Bonn, Kirschallee 1, 53115 Bonn, Germany.
authors.forename: Thomas Vitus
authors.initials: TV
authors.lastname: Linnemann
authors.affiliation: Institute of Cellular and Molecular Botany, Department of Ecophysiology, University of Bonn, Kirschallee 1, 53115 Bonn, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Schreiber
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, 53115 Bonn, Germany dbartels@uni-bonn.de.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Bartels
mesh_terms: D050260:Carbohydrate Metabolism; D032181:Craterostigma / Q000201:enzymology / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014174:Transketolase / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D014174:Transketolase
keywords: Octulose; photosynthesis; resurrection plants; sugar transport; transketolase.