title: 7SKiing on chromatin: Move globally, act locally.
abstract: RNA polymerase II (Pol II) pausing at promoter-proximal regions is a highly regulated step in the transcription cycle. Pause release is facilitated by the P-TEFb kinase, which phosphorylates Pol II and negative elongation factors. Recent studies suggest that P-TEFb (as part of the inhibitory 7SK snRNP) is recruited to promoter-proximal regions through interaction with KAP1/TRIM28/TIF1β to facilitate 'on-site' kinase activation and transcription elongation. Here, I discuss features of this model and future challenges to further hone our understanding of transcriptional regulation including Pol II pausing and pause release.
authors.affiliation: a Department of Microbiology , The University of Texas Southwestern Medical Center , Dallas , TX , USA.
authors.forename: Iván
authors.initials: I
authors.lastname: D'Orso
mesh_terms: D002843:Chromatin / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation; D042925:Positive Transcriptional Elongation Factor B / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D014157:Transcription Factors; C118437:negative elongation factor; D042925:Positive Transcriptional Elongation Factor B; D012319:RNA Polymerase II
keywords: 7SK snRNP; KAP1/TRIM28; P-TEFb; RNA polymerase II; transcription elongation