title: Noninvasive prenatal testing (NIPT) in twin pregnancies with treatment of assisted reproductive techniques (ART) in a single center.
abstract: OBJECTIVE
  The objective of the study is to report the performance of noninvasive prenatal testing (NIPT) in twin pregnancies after the treatment of assisted reproductive technology (ART).
  
  
  METHODS
  In two years period, 565 pregnant women with ART twin pregnancies were prospectively tested by NIPT for screening for trisomy 21 (T21), 18 (T18), and 13 (T13) by sequencing cell-free DNA in maternal plasma. Positive NIPT results were confirmed by karyotyping, while negative results were interviewed after delivery. Pregnant decision based on NIPT and confirmation results was discussed during post-test counseling.
  
  
  RESULTS
  In total of 565 cases, NIPT had a failure rate of 0.9% (5/565). Four cases of T21 were identified by NIPT and confirmed by karyotyping, resulting in 100% (95%CI 39.8%-100%) positive predictive value. Among 556 cases with NIPT negative results, 506 cases (91.0%) were confirmed by follow-up of postnatal phenotypes, while 33 cases (5.9%) had adverse pregnant outcomes with unconfirmed reasons because of the lack of cytogenetic samples. The remaining 17 cases (3.1%) refused follow-up. No false negative result was reported.
  
  
  CONCLUSIONS
  With apparently high positive predictive value and low false positive rate, NIPT has the potential to be used as a good alternative approach of conventional prenatal screening at the first trimester in ART twin pregnancy. © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China.|Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, Changsha, China.|Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Tan
authors.affiliation: BGI-Shenzhen, Shenzhen, China.|China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China.|Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, Changsha, China.|Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
authors.affiliation: Clinical laboratory of BGI Health, BGI-Shenzhen, Shenzhen, China.
authors.forename: Meili
authors.initials: M
authors.lastname: Fu
authors.affiliation: Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Li
authors.affiliation: BGI-Shenzhen, Shenzhen, China.|China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen, China.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Yin
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China.|Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, Changsha, China.|Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Clinical Laboratory of BGI Health, BGI-Wuhan, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China.|Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, Changsha, China.|Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Clinical Laboratory of BGI Health, BGI-Wuhan, Wuhan, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Peng
authors.affiliation: Clinical laboratory of BGI Health, BGI-Shenzhen, Shenzhen, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: BGI-Shenzhen, Shenzhen, China.|China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen, China.|Section of Molecular Disease Biology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Clinical laboratory of BGI Health, BGI-Shenzhen, Shenzhen, China.
authors.forename: Fuman
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Clinical laboratory of BGI Health, BGI-Shenzhen, Shenzhen, China.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, Changsha, China.|Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Lu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China.|Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, Changsha, China.|Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gong
authors.affiliation: BGI-Shenzhen, Shenzhen, China.|Clinical laboratory of BGI Health, BGI-Shenzhen, Shenzhen, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000328:Adult; D002681:China; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D004247:DNA / Q000097:blood* / Q000235:genetics; D004314:Down Syndrome / Q000175:diagnosis / Q000235:genetics; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007621:Karyotyping; D011237:Predictive Value of Tests; D011247:Pregnancy; D059285:Pregnancy, Twin; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted; D017422:Sequence Analysis, DNA / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic; D014314:Trisomy / Q000175:diagnosis / Q000235:genetics; D000073839:Trisomy 13 Syndrome; D000073842:Trisomy 18 Syndrome; D055815:Young Adult
chemical_list: D004247:DNA