title: [Determination of benzyl glucosinolate in Lepidium meyenii from different regions by HPLC].
abstract: The content of benzyl isothiocyanate (BITC) which as the enzymatic hydrolysis product of benzyl glucosinolate through thioglucosidase was determined by HPLC. The content of benzyl isothiocyanate (BITC) which as the enzymatic hydrolysis product of benzyl glucosinolate through thioglucosidase was determined by HPLC. The chromatography condition was as follows: Kaseisorb LC ODS 2000 (4.6 mm x 150 mm, 5 min) column with the mobile phase of acetonitrile(A)-water( B) under gradient elution (0-5 min, 3%-8% A; 5-9 min, 8%-48% A; 9-23 min, 48%-62% A; 23-28 min, 62%-99% A); the flow rate was 1.0 mL x min(-1) with 10 microL injection volume; detection wavelength was 246 nm and temperature of column was 40 degrees C. The content of benzyl glucosinolate was in the range of 10.76-17.91 g x L(-1). The method is simple, accurate and good reproducibility which can be used for the determination of benzyl glucosinolate in Lepidium meyenii, effectively.
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Hong-jun
authors.initials: HJ
authors.lastname: Yin
authors.forename: Cong-cong
authors.initials: CC
authors.lastname: Si
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Hu
authors.forename: Zheng-hai
authors.initials: ZH
authors.lastname: Long
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005961:Glucosinolates / Q000032:analysis*; D029686:Lepidium / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D005961:Glucosinolates; D010936:Plant Extracts