title: Effect of calcium phosphate ceramic substrate geometry on mesenchymal stromal cell organization and osteogenic differentiation.
abstract: The composition of calcium phosphate (CaP) ceramics in combination with surface features have been shown to influence biological performance, and micro- and nano-scale topography is known to stimulate osteogenic differentiation of mesenchymal stromal cells (MSCs). In view of this, adipose tissue derived MSCs were cultured on CaP disks featuring hemispherical concavities of various sizes (440, 800 or 1800 μm diameter). It was hypothesized that (i) surface concavities would promote cell proliferation, cellular organization within the concavities, and osteogenic differentiation, as a result of a more pronounced 3D micro-environment and CaP nucleation in concavities, and (ii) MSC proliferation and osteogenic differentiation would increase with smaller concavity size due to more rapidly occurring 3D cell-cell interactions. We found that concavities indeed affect cell proliferation, with 440 μm concavities increasing cell proliferation to a larger extent compared to 800 and 1800 μm concavities as well as planar surfaces. Additionally, concavity size influenced 3D cellular organization within the concavity volume. Interestingly, concavity size promoted osteogenic differentiation of cells, as evidenced by increased osteocalcin gene expression in 440 μm concavities, and osteocalcin staining predominantly for 440 and 800 μm concavities, but not for 1800 μm concavities and only slightly for planar surface controls.
authors.affiliation: Department of Biomaterials (309), Radboud University Medical Center, Philips van Leijdenlaan 25, 6525 EX Nijmegen, The Netherlands.
authors.forename: Eva R
authors.initials: ER
authors.lastname: Urquia Edreira
authors.forename: Astghik
authors.initials: A
authors.lastname: Hayrapetyan
authors.forename: Joop G C
authors.initials: JG
authors.lastname: Wolke
authors.forename: Huib J E
authors.initials: HJ
authors.lastname: Croes
authors.forename: Alexey
authors.initials: A
authors.lastname: Klymov
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: Jeroen J J P
authors.initials: JJ
authors.lastname: van den Beucken
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D015675:Osteocalcin