title: PNPLA3 Expression Is Related to Liver Steatosis in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease.
abstract: Recent reports suggest a role for the Patatin-like phospholipase domain-containing protein 3 (PNPLA3) in the pathology of non-alcoholic fatty liver disease (NAFLD). Lipid deposition in the liver seems to be a critical process in the pathogenesis of NAFLD. The aim of the present work was to evaluate the association between the liver PNPLA3 expression, key genes of lipid metabolism, and the presence of NAFLD in morbidly obese women. We used real-time polymerase chain reaction (PCR) analysis to analyze the hepatic expression of PNPLA3 and lipid metabolism-related genes in 55 morbidly obese subjects with normal liver histology (NL, n = 18), simple steatosis (SS, n = 20), and non-alcoholic steatohepatitis (NASH, n = 17). Liver biopsies were collected during bariatric surgery. We observed that liver PNPLA3 expression was increased in NAFLD than in NL. It was also upregulated in SS than in NL. Interestingly, we found that the expression of PNPLA3 was significantly higher in severe than mild SS group. In addition, the expression of the transcription factors LXRα, PPARα, and SREBP2 was positively correlated with PNPLA3 liver expression. Regarding rs738409 polymorphism, GG genotype was positive correlated with the presence of NASH. In conclusion, our results show that PNPLA3 could be related to lipid accumulation in liver, mainly in the development and progression of simple steatosis.
authors.affiliation: Group de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Institut Investigació Sanitària Pere Virgili (IISPV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. gemma.aragones@iispv.cat.
authors.forename: Gemma
authors.initials: G
authors.lastname: Aragonès
authors.affiliation: Group de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Institut Investigació Sanitària Pere Virgili (IISPV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. tauguet.hj23.ics@gencat.cat.|Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. tauguet.hj23.ics@gencat.cat.
authors.forename: Teresa
authors.initials: T
authors.lastname: Auguet
authors.affiliation: Group de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Institut Investigació Sanitària Pere Virgili (IISPV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. sandra.armengol@urv.cat.
authors.forename: Sandra
authors.initials: S
authors.lastname: Armengol
authors.affiliation: Group de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Institut Investigació Sanitària Pere Virgili (IISPV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. alba.berlanga@urv.cat.
authors.forename: Alba
authors.initials: A
authors.lastname: Berlanga
authors.affiliation: Group de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Institut Investigació Sanitària Pere Virgili (IISPV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. esther.guiu@urv.cat.
authors.forename: Esther
authors.initials: E
authors.lastname: Guiu-Jurado
authors.affiliation: Group de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Institut Investigació Sanitària Pere Virgili (IISPV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. caguilar.hj23.ics@gencat.cat.
authors.forename: Carmen
authors.initials: C
authors.lastname: Aguilar
authors.affiliation: Servei Anatomia Patològica, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. mgonzalez.hj23.ics@gencat.cat.
authors.forename: Salomé
authors.initials: S
authors.lastname: Martínez
authors.affiliation: Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Avinguda Doctor Josep Laporte, 2, 43204 Tarragona, Spain. fatima.sabench@urv.cat.
authors.forename: Fátima
authors.initials: F
authors.lastname: Sabench
authors.affiliation: Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. aporras.hj23.ics@gencat.cat.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Porras
authors.affiliation: Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. drgorrin@yahoo.es.
authors.forename: Maikel Daniel
authors.initials: MD
authors.lastname: Ruiz
authors.affiliation: Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Avinguda Doctor Josep Laporte, 2, 43204 Tarragona, Spain. mhernandezg@grupsagessa.com.
authors.forename: Mercé
authors.initials: M
authors.lastname: Hernández
authors.affiliation: Servei Anatomia Patològica, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. jsirvent.hj23.ics@gencat.cat.
authors.forename: Joan Josep
authors.initials: JJ
authors.lastname: Sirvent
authors.affiliation: Servei de Cirurgia, Hospital Sant Joan de Reus, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Avinguda Doctor Josep Laporte, 2, 43204 Tarragona, Spain. ddelcastillo@grupsagessa.com.
authors.forename: Daniel
authors.initials: D
authors.lastname: Del Castillo
authors.affiliation: Group de Recerca GEMMAIR (AGAUR)-Medicina Aplicada, Institut Investigació Sanitària Pere Virgili (IISPV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), 43007 Tarragona, Spain. crichart.hj23.ics@gencat.cat.|Servei Medicina Interna, Hospital Universitari Joan XXIII Tarragona, Mallafré Guasch, 4, 43007 Tarragona, Spain. crichart.hj23.ics@gencat.cat.
authors.forename: Cristóbal
authors.initials: C
authors.lastname: Richart
mesh_terms: D000483:Alleles; D015331:Cohort Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D008099:Liver / Q000378:metabolism / Q000473:pathology; D000071518:Liver X Receptors / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000150:complications / Q000235:genetics / Q000473:pathology*; D009767:Obesity, Morbid / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
chemical_list: D008049:Lipase; D000071518:Liver X Receptors; D008565:Membrane Proteins; D047493:PPAR alpha; D051782:Sterol Regulatory Element Binding Protein 2; C533682:PNPLA3 protein, human; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
keywords: PNPLA3; fatty acid metabolism; morbid obesity; non-alcoholic fatty liver disease; non-alcoholic steatohepatitis; simple steatosis