title: The role of glutamine in Pseudomonas mediterranea in biotechnological processes.
abstract: In this work, in order to study the effect of glutamine as co-feeder on growth kinetics, biomass and PHA production in Pseudomonas mediterranea, different co-metabolic strategies were employed. Unrelated (glycerol and glucose) and related (sodium octanoate) carbon sources both in presence and absence of glutamine have been tested. For each cultural condition, we (i) evaluated growth kinetics and measured the cell metabolic activity by MTT assay, (ii) monitored PHA production and (iii) estimated the expression of phaC1 and phaC2 genes through RT-PCR. Our results show that the use of glutamine as co-feeder in P. mediterranea led to an improvement of the specific growth rate and cell metabolic activity and enhanced the uptake of all the carbon sources assayed. Moreover, the use of glutamine reduced significantly the time required for PHA production and increased biopolymer yield, as consequence of an early activation of phaC1 and phaC2.
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina Viale F. Stagno D' Alcontres, 98166, Messina Italy.
authors.forename: Maria Giovanna
authors.initials: MG
authors.lastname: Rizzo
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina Viale F. Stagno D' Alcontres, 98166, Messina Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Chines
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina Viale F. Stagno D' Alcontres, 98166, Messina Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Franco
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina Viale F. Stagno D' Alcontres, 98166, Messina Italy.
authors.forename: Marco S
authors.initials: MS
authors.lastname: Nicolò
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina Viale F. Stagno D' Alcontres, 98166, Messina Italy. Electronic address: sguglielm@unime.it.
authors.forename: Salvatore P P
authors.initials: SP
authors.lastname: Guglielmino
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D002244:Carbon / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005973:Glutamine / Q000378:metabolism*; D007700:Kinetics; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D005973:Glutamine; D002244:Carbon