title: An engineered non-oxidative glycolysis pathway for acetone production in Escherichia coli.
abstract: OBJECTIVE
  To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli.
  
  
  RESULTS
  Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical acetone yield from 1 to 1.5 mol acetone/mol glucose. By inserting the fxpk gene encoding phosphoketolase from Bifidobacterium adolescentis into the genome, we constructed a NOG pathway in E.coli. The resulting strain produced 47 mM acetone from glucose under aerobic conditions in shake-flasks. The yield of acetone was improved from 0.38 to 0.47 mol acetone/mol glucose which is a significant over the parent strain.
  
  
  CONCLUSIONS
  Guided by computational analysis of metabolic networks, we introduced a NOG pathway into E. coli and increased the yield of acetone, which demonstrates the importance of modeling analysis for the novel metabolic engineering strategies.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, People's Republic of China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, People's Republic of China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, People's Republic of China. ma_hw@tib.cas.cn.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Ma
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000096:Acetone / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology; D060847:Metabolic Engineering
chemical_list: D000096:Acetone
keywords: Acetone; Escherichia coli; Non-oxidative glycolysis; Phosphoketolase