title: The Effects of Thermal Preconditioning on Oncogenic and Intraspinal Cord Growth Features of Human Glioma Cells.
abstract: The adult rodent spinal cord presents an inhibitory environment for donor cell survival, impeding efficiency for xenograft-based modeling of gliomas. We postulated that mild thermal preconditioning may influence the fate of the implanted tumor cells. To test this hypothesis, high-grade human astrocytoma G55 and U87 cells were cultured under 37C and 38.5C to mimic regular experimental or core body temperatures of rodents, respectively. In vitro, the 38.5C-conditioned cells, relative to 37C, grew slightly faster. Compared to U87 cells, G55 cells demonstrated a greater response to the temperature difference. Hyperthermal culture markedly increased production of Hsp27 in most G55 cells, but only promoted transient expression of cancer stem cell marker CD133 in a small cell subpopulation. We subsequently transplanted G55 cells following 37C or 38.5C culture into the C2 or T10 spinal cord of adult female immunodeficient rats (3 rats/each locus/per temperature; total: 12 rats). Systematic analyses revealed that 38.5C-preconditioned G55 cells grew more malignantly at either C2 or T10 as determined by tumor size, outgrowth profile, resistance to bolus intratumor administration of 5-fluorouracil (0.1 mol), and posttumor survival (p0.05; n=6/group). Therefore, thermal preconditioning of glioma cells may be an effective way to influence the in vitro and in vivo oncological contour of glioma cells. Future studies are needed for assessing the potential oncogenic modifying effect of hyperthermia regimens on glioma cells.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zeng
authors.forename: Inbo
authors.initials: I
authors.lastname: Han
authors.forename: Muhammad
authors.initials: M
authors.lastname: Abd-El-Barr
authors.forename: Zaid
authors.initials: Z
authors.lastname: Aljuboori
authors.forename: Jamie E
authors.initials: JE
authors.lastname: Anderson
authors.forename: John H
authors.initials: JH
authors.lastname: Chi
authors.forename: Ross D
authors.initials: RD
authors.lastname: Zafonte
authors.forename: Yang D
authors.initials: YD
authors.lastname: Teng
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001254:Astrocytoma / Q000378:metabolism / Q000473:pathology; D001831:Body Temperature / Q000502:physiology*; D001932:Brain Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013120:Spinal Cord Neoplasms / Q000378:metabolism / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000071916:AC133 Antigen; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins