title: Label-Free Quantitative Proteomics Reveals Differences in Molecular Mechanism of Atherosclerosis Related and Non-Related to Chronic Kidney Disease.
abstract: The major cause of mortality in patients with chronic kidney disease (CKD) is atherosclerosis related to traditional and non-traditional risk factors. However, the understanding of the molecular specificity that distinguishes the risk factors for classical cardiovascular disease (CVD) and CKD-related atherosclerosis (CKD-A) is far from complete. In this study we investigated the disease-related differences in the proteomes of patients with atherosclerosis related and non-related to CKD. Plasma collected from patients in various stages of CKD, CVD patients without symptoms of kidney dysfunction, and healthy volunteers (HVs), were analyzed by a coupled label-free and mass spectrometry approach. Dysregulated proteins were confirmed by an enzyme-linked immunosorbent assay (ELISA). All proteomic data were correlated with kidney disease development and were subjected to bioinformatics analysis. One hundred sixty-two differentially expressed proteins were identified. By directly comparing the plasma proteomes from HVs, CKD, and CVD patients in one study, we demonstrated that proteins involved in inflammation, blood coagulation, oxidative stress, vascular damage, and calcification process exhibited greater alterations in patients with atherosclerosis related with CKD. These data indicate that the above nontraditional risk factors are strongly specific for CKD-A and appear to be less essential for the development of "classical" CVD.
authors.affiliation: European Centre for Bioinformatics and Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. magdalena.luczak@put.poznan.pl.|Institute of Chemical Technology and Engineering, Poznan University of Technology, Piotrowo 3, 60-965 Poznan, Poland. magdalena.luczak@put.poznan.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Luczak
authors.affiliation: Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland. suszynska@yahoo.co.uk.
authors.forename: Joanna
authors.initials: J
authors.lastname: Suszynska-Zajczyk
authors.affiliation: European Centre for Bioinformatics and Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. lukasmar@ibch.poznan.pl.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Marczak
authors.affiliation: Department of Clinical Biochemistry and Laboratory Medicine, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland. doforman@ump.edu.pl.
authors.forename: Dorota
authors.initials: D
authors.lastname: Formanowicz
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland. epawliczak@wp.pl.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pawliczak
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland. wanic.kossowska.maria@gmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Wanic-Kossowska
authors.affiliation: European Centre for Bioinformatics and Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. mackis@ibch.poznan.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Stobiecki
mesh_terms: D050197:Atherosclerosis / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D053495:Osteopontin / Q000378:metabolism; D054464:Peroxiredoxins / Q000378:metabolism; D025341:Principal Component Analysis; D040901:Proteomics; D051436:Renal Insufficiency, Chronic / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D012307:Risk Factors; D053719:Tandem Mass Spectrometry; D060749:alpha-2-HS-Glycoprotein / Q000378:metabolism
chemical_list: D060749:alpha-2-HS-Glycoprotein; D053495:Osteopontin; D054464:Peroxiredoxins
keywords: atherosclerosis; cardiovascular disease; chronic kidney disease; label-free quantitative proteomics