title: Complete NMR assignment of a bisecting hybrid-type oligosaccharide transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase.
abstract: This study describes the complete nuclear magnetic resonance (NMR) spectral assignment of a bisecting hybrid-type oligosaccharide 1, transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M). Through (1)H- and (13)C-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments, we determine the structure of the glycoside linkage formed by the Endo-M transglycosylation, i.e., the connection between GlcNAc and GlcNAc in oligosaccharide 1.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan. Electronic address: yamanoi1119@gmail.com.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanoi
authors.affiliation: Technology Joint Management Office, Tokai University, 4-1-1 Kitanakame, Hiratsuka, Kanagawa 259-1292, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Oda
authors.affiliation: Department of Human Ecology, Wayo Women's University, Chiba 272-8533, Japan.
authors.forename: Kaname
authors.initials: K
authors.lastname: Katsuraya
authors.affiliation: Department of Applied Chemistry, School of Engineering, Institute of Glycoscience, Tokai University, 4-1-1 Kitanakame, Hiratsuka, Kanagawa 259-1292, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Inazu
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Ishikawa 921-8836, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D002240:Carbohydrate Sequence; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism*; D009089:Mucor / Q000201:enzymology*; D009844:Oligosaccharides / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D009844:Oligosaccharides; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
keywords: Bisecting hybrid-type oligosaccharide; Endo-M; Endo-β-N-acetylglucosaminidase; NMR spectral assignment; Transglycosylation product