title: Simultaneous determination of 22 cephalosporins drug residues in pork muscle using liquid chromatography-tandem mass spectrometry.
abstract: A simple, sensitive and reliable analytical method was developed for the simultaneous determination of 22 common cephalosporins from the first generation to the fourth generation in pork muscle by liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method. Under the optimized extraction conditions, samples were directly purified through membrane filtration to separate all 22 cephalosporins and the critical pairs of each parent drug were completely separated. Variables affecting the LC-MS/MS were optimized to get a better separation. The excellent selectivity and sensitivity achieved in multiple reactions monitoring (MRM) mode allowed satisfactory confirmation and quantitation for the 22 cephalosporins. The linear range of the 22 cephalosporins is 0.06-100.0μg/L with good correlation coefficients (r(2)>0.9920). The limits of detection (LODs) and limits of quantitation (LOQs) of these compounds were in the range 0.04-3.0μg/L and 0.06-10.0μg/kg, respectively. The average intra-day recoveries at 3 spiked levels (LOQ, 2LOQ, 4LOQ) were all in the range 83.6-113.0% with RSDs (n=6) lower than 6.5%. The method of LC-MS/MS developed in this study was initially applied to the research of 22 cephalosporins in 12 retail pork samples and proved to be accurate, sensitive, minimum sample pre-treatment, convenient and practical.
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Li
authors.affiliation: CAS key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China; School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Shen
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China; College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China; School of pharmacy, China Medical University, Shenyang 110013, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Electronic address: fengzhang@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002511:Cephalosporins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D000069466:Red Meat / Q000032:analysis*; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D002511:Cephalosporins
keywords: Cephalosporins; Liquid chromatography; Muscle; Tandem mass spectrometry