title: Transcriptional Regulation of Cytosolic Sulfotransferase 1C2 by Vitamin D Receptor in LS180 Human Colorectal Adenocarcinoma Cells.
abstract: The factors that regulate expression of genes in the 1C family of human cytosolic sulfotransferases (SULT1C) are not well understood. In a recent study evaluating the effects of a panel of transcription factor activators on SULT1C family member expression in LS180 human colorectal adenocarcinoma cells, we found that SULT1C2 expression was significantly increased by 1α,25-dihydroxyvitamin D3 (VitD3) treatment. The objective of our current study was to identify the mechanism responsible for VitD3-mediated activation of SULT1C2 transcription. VitD3 treatment of LS180 cells activated transcription of a transfected luciferase reporter plasmid that contained ∼5 kilobase pairs (kbp) of the SULT1C2 gene, which included 402 nucleotides (nt) of the noncoding exon 1, all of intron 1, and 21 nt of exon 2. Although computational analysis of the VitD3-responsive region of the SULT1C2 gene identified a pregnane X receptor (PXR)-binding site within exon 1, the transfected 5 kbp SULT1C2 reporter was not activated by treatment with rifampicin, a prototypical PXR agonist. However, deletion or mutation of the predicted PXR-binding site abolished VitD3-mediated SULT1C2 transcriptional activation, identifying the site as a functional vitamin D response element (VDRE). We further demonstrated that vitamin D receptor (VDR) can interact directly with the SULT1C2 VDRE sequence using an enzyme-linked immunosorbent assay-based transcription factor binding assay. In conclusion, VitD3-inducible SULT1C2 transcription is mediated through a VDRE in exon 1. These results suggest a role for SULT1C2 in VitD3-regulated physiologic processes in human intestine.
authors.affiliation: Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan.
authors.forename: Kathleen G
authors.initials: KG
authors.lastname: Barrett
authors.affiliation: Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan.
authors.forename: Hailin
authors.initials: H
authors.lastname: Fang
authors.affiliation: Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Kocarek
authors.affiliation: Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan m.runge-morris@wayne.edu.
authors.forename: Melissa
authors.initials: M
authors.lastname: Runge-Morris
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D001665:Binding Sites; D002117:Calcitriol / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018167:Receptors, Calcitriol / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D015238:Sulfotransferases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D015533:Transcriptional Activation / Q000187:drug effects*; D014162:Transfection; D030181:Vitamin D Response Element
chemical_list: D018167:Receptors, Calcitriol; C579457:VDR protein, human; C513319:SULT1C2 protein, human; D015238:Sulfotransferases; D002117:Calcitriol