title: Encapsulation of anti-carbonic anhydrase IX antibody in hydrogel microspheres for tumor targeting.
abstract: Encapsulation is a well-established method of biomaterial protection, controlled release, and efficient delivery. Here we evaluated encapsulation of monoclonal antibody M75 directed to tumor biomarker carbonic anhydrase IX (CA IX) into alginate microbeads (SA-beads) or microcapsules made of sodium alginate, cellulose sulfate, and poly(methylene-co-guanidine) (PMCG). M75 antibody release was quantified using ELISA and its binding properties were assessed by immunodetection methods. SA-beads showed rapid M75 antibody release in the first hour, followed by steady release during the whole experiment of 7 days. In contrast, the M75 release from PMCG capsules was gradual, reaching the maximum concentration on the 7th day. The release was more efficient at pH 6.8 compared to pH 7.4. The released antibody could recognize CA IX, and target the CA IX-positive cells in 3D spheroids. In conclusion, SA-beads and PMCG microcapsules can be considered as promising antibody reservoirs for targeting of cancer cells.
authors.affiliation: a Department of Molecular Medicine , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia.|b Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute , Brno , Czech Republic , and.
authors.forename: Martina
authors.initials: M
authors.lastname: Takacova
authors.affiliation: c Department for Biomaterials Research , Polymer Institute, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Hlouskova
authors.affiliation: a Department of Molecular Medicine , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Miriam
authors.initials: M
authors.lastname: Zatovicova
authors.affiliation: a Department of Molecular Medicine , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Martin
authors.initials: M
authors.lastname: Benej
authors.affiliation: a Department of Molecular Medicine , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Olga
authors.initials: O
authors.lastname: Sedlakova
authors.affiliation: a Department of Molecular Medicine , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Juraj
authors.initials: J
authors.lastname: Kopacek
authors.affiliation: a Department of Molecular Medicine , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Pastorek
authors.affiliation: c Department for Biomaterials Research , Polymer Institute, Slovak Academy of Sciences , Bratislava , Slovakia.
authors.forename: Igor
authors.initials: I
authors.lastname: Lacik
authors.affiliation: a Department of Molecular Medicine , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences , Bratislava , Slovakia.|b Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute , Brno , Czech Republic , and.
authors.forename: Silvia
authors.initials: S
authors.lastname: Pastorekova
mesh_terms: D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology / Q000493:pharmacokinetics*; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000008:administration & dosage; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D000071231:Carbonic Anhydrase IX / Q000276:immunology* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D018874:Spheroids, Cellular / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C475102:M75 monoclonal antibody; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX
keywords: Antibody delivery; cancer; carbonic anhydrase IX; controlled release; encapsulation