title: [Preliminary analysis on spectrum-efficient correlation model for anti-influenza virus of Lonicerae Japonicae Flos by partial least squares method].
abstract: Forty batches of Lonicerae Japonica Fse i collected extensively and prepared as the test solution. Their chromatographic fingerprints and anti-influenza virus IC50 value (half maximal inhibitory concentration) were determined respectively. Then Unscrambler software was used, and spectrum-efficient correlation analysis was done for chromatographic fingerprints data and IC50 data by partial least squares regression method, to establish spectrum-efficient correlation model for anti-influenza virus of Lonicerae Japonicae Flos. Then the other 10 batches of Lonicerae Japonicae Flos were used to verify the model and explore the adaptability of this spectrum-efficient correlation model based on partial least squares regression method. The mathematical model obtained R2 of 0.969489 and RM-SEC of 0.070691 for calibration set; R2 of 0.959042 and RMSECV of 0.084005 for cross validation set. The verification experiment results showed that the relative error between the predicted values and measured values was within 10% in all 10 hatches, and within 5% in 80% of them. The results showed that the established spectrum-efficient correlation model could be used to evaluate the biological activity of anti-influenza virus of Lonicerae Japonicae Flos by determining its HPLC fingerprints.
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Mi
authors.forename: Young-xiang
authors.initials: YX
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Meng
authors.forename: Xiu-hai
authors.initials: XH
authors.lastname: Wang
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Tao
authors.forename: Zhen-zhon
authors.initials: ZZ
authors.lastname: Wang
mesh_terms: D000998:Antiviral Agents / Q000032:analysis / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D016018:Least-Squares Analysis; D031239:Lonicera / Q000737:chemistry*; D015394:Molecular Structure; D009975:Orthomyxoviridae / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal