title: Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens.
abstract: In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used fast protein liquid chromatography (FPLC)-fractionated extracts of F. hepatica excretory/secretory antigens (E/S Ags) for serodiagnosis of human and sheep fasciolosis. To develop an improved diagnostic method, we fractionated F. hepatica E/S Ags by anion exchange chromatography on a Sepharose CL-6B column and then tested the serodiagnostic values of the fractions. We used sera from F. hepatica-infected human and sheep as positive controls. Sera from patients with hydatidosis and strongyloidiasis were used for cross-reactivity studies. Enzyme-linked immunosorbent assays (ELISA) of the second FPLC peak, containing 20, 25, and 70 kDa proteins, discriminated between F. hepatica-infected and uninfected human and sheep samples. Fractionation of F. hepatica E/S Ags by FPLC is a fast and reproducible way of obtaining antigens useful for serodiagnosis of human and sheep fasciolosis with acceptable sensitivity and specificity. Graphical abstract ᅟ.
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
authors.forename: Kobra
authors.initials: K
authors.lastname: Mokhtarian
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran. Akhlaghi.l@iums.ac.ir.
authors.forename: Lame
authors.initials: L
authors.lastname: Akhlaghi
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Meamar
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Razmjou
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Shahrekord University of Medical Science, Shahrekord, Iran.
authors.forename: Kourosh
authors.initials: K
authors.lastname: Manouchehri Naeini
authors.affiliation: Immunology Research Center, Iran University of Medical Science (IUMS), Tehran, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Gholami
authors.affiliation: Immunology Research Center, Iran University of Medical Science (IUMS), Tehran, Iran.|Department of Immunology, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Najafi Samei
authors.affiliation: Immunology Research Center, Iran University of Medical Science (IUMS), Tehran, Iran. Falak.r@IUMS.ac.ir.|Department of Immunology, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran. Falak.r@IUMS.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Falak
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000662:veterinary; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005210:Fasciola hepatica / Q000276:immunology; D005211:Fascioliasis / Q000175:diagnosis* / Q000662:veterinary; D006801:Humans; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods* / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth
keywords: Fasciolosis; Fast protein liquid chromatography; Fractionated excretory/secretory antigens