title: Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry.
abstract: Proteomic analysis was employed to map the seed storage protein network in landrace and cultivated chickpea accessions. Protein extracts were separated by two-dimensional gel electrophoresis (2D-GE) across a broad range 3.0-10.0 immobilized pH gradient (IPG) strips. Comparative elucidation of differentially expressed proteins between two diverse geographically originated chickpea accessions was carried out using 2D-GE coupled with mass spectrometry. A total of 600 protein spots were detected in these accessions. In-gel protein expression patterns revealed three protein spots as upregulated and three other as downregulated. Using trypsin in-gel digestion, these differentially expressed proteins were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) which showed 45% amino acid homology of chickpea seed storage proteins with Arabidopsis thaliana.
authors.affiliation: School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, India.
authors.forename: Pramod Kumar
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Department of Bioscience & Biotechnology, Banasthali University, Banasthali 304022, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Shrivastava
authors.affiliation: Defense Research & Development Establishment, Gwalior 474011, India.
authors.forename: Krishna
authors.initials: K
authors.lastname: Chaturvedi
authors.affiliation: Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
authors.forename: Bechan
authors.identifier: 0000-0002-7835-9406
authors.initials: B
authors.lastname: Sharma
authors.affiliation: School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, India.
authors.forename: Sameer S
authors.identifier: 0000-0002-4405-5930
authors.initials: SS
authors.lastname: Bhagyawant