title: Incidence of Sperm Surface Autoantibodies and Relationship with Routine Semen Parameters and Sperm Kinematics.
abstract: Antisperm antibodies (ASA) are associated with male subfertility. However, results on sperm surface autoantibodies are controversial, the relationship between ASA and semen parameters (WHO, 2010) is unknown, and data on ASA and sperm kinematics are scarce.
  
  
  
  A retrospective study carried out in men undergoing routine semen analysis (WHO 2010), ASA evaluation (direct SpermMAR(™) (IgG) test), and computer-assisted sperm analysis (CASA).
  
  
  
  A 2.6% and a 5.9% incidence of ASA-positive cases were found (cut-off 50% and 10%, respectively; n = 7492). ASA-positive samples had lower (P < 0.0001) sperm concentration, count, motility, and hypo-osmotic swelling (HOS) test score. HOS results did not correlate with sperm vitality in normozoospermic samples with high ASA levels. In unselected samples, ASA-positive samples (cut-off 50%) showed decreased sperm kinematics (VSL, VAP, LIN, ALH, STR, BCF, WOB), but in normozoospermic samples, ASA-positive and ASA-negative subgroups had similar CASA results.
  
  
  
  ASA evaluation is highly relevant in full semen assessment.
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME; CONICET-FIBYME), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Gustavo Luis
authors.initials: GL
authors.lastname: Verón
authors.affiliation: Laboratorio de Andrología y Reproducción, Córdoba, Argentina.
authors.forename: Rosa Isabel
authors.initials: RI
authors.lastname: Molina
authors.affiliation: Laboratorio de Andrología y Reproducción, Córdoba, Argentina.
authors.forename: Andrea Daniela
authors.initials: AD
authors.lastname: Tissera
authors.affiliation: Centro Integral de Ginecología, Obstetricia y Reproducción (CIGOR), Córdoba, Argentina.
authors.forename: Gustavo Martín
authors.initials: GM
authors.lastname: Estofan
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME; CONICET-FIBYME), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Clara Isabel
authors.initials: CI
authors.lastname: Marín-Briggiler
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME; CONICET-FIBYME), Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Mónica Hebe
authors.initials: MH
authors.lastname: Vazquez-Levin
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D013081:Sperm Motility / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G
keywords: Antisperm antibodies; MAR test; computer-assisted sperm analysis; human sperm; hypo-osmotic swelling test; semen parameters