title: 4-Hydroxy-2,3-Dimethyl-2-Nonen-4-Olide Has an Inhibitory Effect on Pro-Inflammatory Cytokine Production in CpG-Stimulated Bone Marrow-Derived Dendritic Cells.
abstract: This study was intended to assess the anti-inflammatory properties of 4-hydroxy-2,3-dimethyl-2-nonen-4-olide (Comp) isolated from Ulva pertusa Kjellman on production of pro-inflammatory cytokines. Comp revealed remarkable inhibitory effects on production of pro-inflammatory cytokines in bone marrow-derived dendritic cells (BMDCs). Comp pre-treatment in the CpG DNA-stimulated BMDCs exhibited strong inhibition of interleukin (IL)-12 p40 and IL-6 production with IC50 values ranging from 7.57 ± 0.2 to 10.83 ± 0.3, respectively. It revealed an inhibitory effect on the phosphorylation of ERK1/2, JNK1/2, and p38, and on activator protein (AP)-1 reporter activity. Comp displayed noteworthy inhibitory effects on phosphorylation and degradation of IκBα, and on NF-κB reporter activity. In summary, these data propose that Comp has substantial anti-inflammatory properties and warrants further study concerning its potential use as a therapeutic agent for inflammation-associated maladies.
authors.affiliation: School of Medicine and Brain Korea 21 PLUS Program, Jeju National University, Jeju 690-756, Korea. whitebrands57@yahoo.com.|Institute of Medical Science, Jeju National University, Jeju 690-756, Korea. whitebrands57@yahoo.com.
authors.forename: Zahid
authors.initials: Z
authors.lastname: Manzoor
authors.affiliation: School of Medicine and Brain Korea 21 PLUS Program, Jeju National University, Jeju 690-756, Korea. hohoje@nate.com.|Institute of Medical Science, Jeju National University, Jeju 690-756, Korea. hohoje@nate.com.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Koo
authors.affiliation: School of Medicine and Brain Korea 21 PLUS Program, Jeju National University, Jeju 690-756, Korea. irshad.qau200@gmail.com.|Institute of Medical Science, Jeju National University, Jeju 690-756, Korea. irshad.qau200@gmail.com.
authors.forename: Irshad
authors.initials: I
authors.lastname: Ali
authors.affiliation: Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 690-756, Korea. jungeun0615@nate.com.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 690-756, Korea. saeukkang@jejunu.ac.kr.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Byeon
authors.affiliation: School of Medicine and Brain Korea 21 PLUS Program, Jeju National University, Jeju 690-756, Korea. eunsyoo@jejunu.ac.kr.|Institute of Medical Science, Jeju National University, Jeju 690-756, Korea. eunsyoo@jejunu.ac.kr.
authors.forename: Eun-Sook
authors.initials: ES
authors.lastname: Yoo
authors.affiliation: School of Medicine and Brain Korea 21 PLUS Program, Jeju National University, Jeju 690-756, Korea. pharmkhk@jejunu.ac.kr.|Institute of Medical Science, Jeju National University, Jeju 690-756, Korea. pharmkhk@jejunu.ac.kr.
authors.forename: Hee-Kyoung
authors.initials: HK
authors.lastname: Kang
authors.affiliation: School of Medicine and Brain Korea 21 PLUS Program, Jeju National University, Jeju 690-756, Korea. jinwonh@jejunu.ac.kr.|Institute of Medical Science, Jeju National University, Jeju 690-756, Korea. jinwonh@jejunu.ac.kr.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Hyun
authors.affiliation: Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 690-756, Korea. namho@jejunu.ac.kr.
authors.forename: Nam-Ho
authors.initials: NH
authors.lastname: Lee
authors.affiliation: School of Medicine and Brain Korea 21 PLUS Program, Jeju National University, Jeju 690-756, Korea. yskoh7@jejunu.ac.kr.|Institute of Medical Science, Jeju National University, Jeju 690-756, Korea. yskoh7@jejunu.ac.kr.
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Koh
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects*; D018899:CpG Islands / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000187:drug effects*; D005260:Female; D007249:Inflammation / Q000188:drug therapy*; D018664:Interleukin-12 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D018808:Transcription Factor AP-1 / Q000378:metabolism; D044445:Ulva / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D015850:Interleukin-6; D016328:NF-kappa B; D010936:Plant Extracts; D018808:Transcription Factor AP-1; D000072000:NF-KappaB Inhibitor alpha; D018664:Interleukin-12
keywords: 4-hydroxy-2,3-dimethyl-2-nonen-4-olide; activator protein-1; inflammation; pro-inflammatory cytokine