title: Upregulation of CDK7 in gastric cancer cell promotes tumor cell proliferation and predicts poor prognosis.
abstract: CDK7 has been known as a component of CDK activating kinase (CAK) complex, the complex was composed of CDK7, Cyclin H and RING finger protein Mat1 that contribute to cell cycle progression by phosphorylating other CDKs. In addition, the complex is also an essential component of general transcription factor TFIIH which controls transcription via activating RNA polymerase II by serines 5 and 7 phosphorylation of the carboxyl-terminal domain (CTD) of its largest subunit. However, the role of CDK7 in the pathogenesis of gastric cancer has not been identified. Our study showed that CDK7 was significantly upregulated and positively correlated with tumor grade, infiltration depth, lymph node, Ki-67, and predicted poor prognosis in 173 gastric cancer specimens by immunohistochemistrical analyses. Furthermore, in vitro results indicated that CDK7 promoted proliferation of gastric cancer cells by CCK8, clone formation analyses and flow cytometric analyses, while CDK7 knockdown led to decreased cell proliferation. Our study will provide a theoretical basis for the study of CDK7 in gastric cancer.
authors.affiliation: Department of Laboratory Medicine, Nantong Tumor Hospital, Nantong 226363, Jiangsu, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Manhua
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Oncology, Nantong Tumor Hospital, Nantong 226363, Jiangsu, China.
authors.forename: Xunlei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ke
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Oncology, Nantong Tumor Hospital, Nantong 226363, Jiangsu, China.
authors.forename: Jinzhang
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Shan
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China. Electronic address: baobojun6@163.com.
authors.forename: Bojun
authors.initials: B
authors.lastname: Bao
authors.affiliation: Department of Oncology, Nantong Tumor Hospital, Nantong 226363, Jiangsu, China. Electronic address: leiyang.53@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation; D000095043:Cyclin-Dependent Kinase-Activating Kinase
chemical_list: D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D018844:Cyclin-Dependent Kinases; D000095043:Cyclin-Dependent Kinase-Activating Kinase; C000722416:CDK7 protein, human
keywords: CDK7; Gastric cancer; Proliferation