title: Genotype, environment and G × E interaction influence (1,3;1,4)-β-d-glucan fine structure in barley (Hordeum vulgare L.).
abstract: BACKGROUND
  The structure of β-glucan influences its use in cereal-based foods and feed. The objective of this study was to determine the effect of environment (E) and genotype (G) on β-glucan fine structure and its genetic control in two-row spring barley with normal starch characteristics.
  
  
  RESULTS
  A population of 89 recombinant inbred lines, derived from the cross of two-row spring barley genotypes Merit × H93174006 (H92076F1 × TR238), was characterized for concentration and structure of grain β-glucan in two environments. Results showed that concentrations of β-glucan, DP3, DP4 and DP3 + DP4 were positively correlated with each other, suggesting no preference for DP3 or DP4 subunit production in high- or low-β-glucan lines. The concentrations of β-glucan, DP3, DP4 and DP3:DP4 ratios were significantly influenced by genotype and environment. However, only DP3:DP4 ratio showed a significant effect of G × E interaction. Association mapping of candidate markers in 119 barley genotypes showed that marker CSLF6_4105 was associated with β-glucan concentration, whereas Bmac504 and Bmac211 were associated with DP3:DP4 ratio. Bmac273e was associated with both β-glucan concentration and DP3:DP4 ratio.
  
  
  CONCLUSIONS
  The grain β-glucan concentration and DP3:DP4 ratio are strongly affected by genotype and environment. Single-marker analyses suggested that the genetic control of β-glucan concentration and DP3:DP4 ratio was linked to separate chromosomal regions on barley genome. © 2016 Society of Chemical Industry.
authors.affiliation: Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
authors.forename: Aron T
authors.initials: AT
authors.lastname: Cory
authors.affiliation: Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
authors.forename: Manu P
authors.initials: MP
authors.lastname: Gangola
authors.affiliation: Alberta Innovates - Technology Futures, Vegreville, Alberta, Canada.
authors.forename: Anthony
authors.initials: A
authors.lastname: Anyia
authors.affiliation: Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
authors.forename: Monica
authors.initials: M
authors.lastname: Båga
authors.affiliation: Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
authors.forename: Ravindra N
authors.initials: RN
authors.lastname: Chibbar
mesh_terms: D000416:Alberta; D000531:Altitude; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002240:Carbohydrate Sequence; D002482:Cellulose / Q000235:genetics / Q000378:metabolism; D002980:Climate; D003433:Crosses, Genetic; D004040:Dietary Carbohydrates / Q000032:analysis* / Q000378:metabolism; D059647:Gene-Environment Interaction; D005819:Genetic Markers; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D001467:Hordeum / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D009753:Nutritive Value; D000069600:Plant Breeding; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013379:Substrate Specificity; D013780:Tetroses / Q000378:metabolism; D014306:Trioses / Q000378:metabolism; D047071:beta-Glucans / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D004040:Dietary Carbohydrates; D005819:Genetic Markers; D010940:Plant Proteins; D013780:Tetroses; D014306:Trioses; D047071:beta-Glucans; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase
keywords: (1,3;1,4)-β-glucan; association mapping; barley; cellotetraose; cellotriose