title: TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulum.
abstract: Procollagens, pre-chylomicrons, and pre-very low-density lipoproteins (pre-VLDLs) are too big to fit into conventional COPII-coated vesicles, so how are these bulky cargoes exported from the endoplasmic reticulum (ER)? We have shown that TANGO1 located at the ER exit site is necessary for procollagen export. We report a role for TANGO1 and TANGO1-like (TALI), a chimeric protein resulting from fusion of MIA2 and cTAGE5 gene products, in the export of pre-chylomicrons and pre-VLDLs from the ER. TANGO1 binds TALI, and both interact with apolipoprotein B (ApoB) and are necessary for the recruitment of ApoB-containing lipid particles to ER exit sites for their subsequent export. Although export of ApoB requires the function of both TANGO1 and TALI, the export of procollagen XII by the same cells requires only TANGO1. These findings reveal a general role for TANGO1 in the export of bulky cargoes from the ER and identify a specific requirement for TALI in assisting TANGO1 to export bulky lipid particles.
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain Universitat Pompeu Fabra, 08002 Barcelona, Spain.
authors.forename: António J M
authors.initials: AJ
authors.lastname: Santos
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain Universitat Pompeu Fabra, 08002 Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Nogueira
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain Universitat Pompeu Fabra, 08002 Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Ortega-Bellido
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain Universitat Pompeu Fabra, 08002 Barcelona, Spain Institució Catalana de Recerca i Estudis Avançats, 08010 Barcelona, Spain vivek.malhotra@crg.eu.
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001055:Apolipoproteins B / Q000378:metabolism; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001343:Autophagy; D018938:Caco-2 Cells; D003094:Collagen / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D017353:Gene Deletion; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D050506:Mutant Chimeric Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: C497396:ARNT protein, human; D000951:Antigens, Neoplasm; D001055:Apolipoproteins B; D008079:Lipoproteins, VLDL; C109278:MIA2 protein, human; D050506:Mutant Chimeric Proteins; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D003094:Collagen