title: Brucella abortus RB51 in milk of vaccinated adult cattle.
abstract: The aim of this study was to evaluate the shedding of Brucella abortus in the milk of cows vaccinated with a full dose of RB51 during lactation. Eighteen cows, nine previously vaccinated with S19 as calves and nine non-vaccinated, were immunized subcutaneously with 1.3×10(10)CFU of B. abortus RB51, 30-60days after parturition. Milk samples from all animals were collected daily until day 7, and at weekly interval for the next 9 weeks after vaccination. To evaluate the shedding of B. abortus, milk samples were submitted for culture and PCR. No B. abortus was isolated from any sample tested. Only one sample, collected on first day after vaccination from a cow previously vaccinated, was faintly positive in the PCR. In conclusion, the public health hazard associated with milk consumption from cows vaccinated with RB51 in post-partum is very low, despite vaccination with the full dose and regardless of previous S19 vaccination.
authors.affiliation: Laboratório de Bacteriologia Aplicada, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Avenida Antônio Carlos 6627, Caixa Postal 567, 31270-901, Belo Horizonte, MG, Brazil. Electronic address: karinalmi@gmail.com.
authors.forename: Karina Leite
authors.initials: KL
authors.lastname: Miranda
authors.affiliation: Laboratório de Bacteriologia Aplicada, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Avenida Antônio Carlos 6627, Caixa Postal 567, 31270-901, Belo Horizonte, MG, Brazil. Electronic address: poesterf@terra.com.br.
authors.forename: Fernando Padilla
authors.initials: FP
authors.lastname: Poester
authors.affiliation: Laboratório de Bacteriologia Aplicada, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Avenida Antônio Carlos 6627, Caixa Postal 567, 31270-901, Belo Horizonte, MG, Brazil; Departamento de Medicina Veterinária, Universidade Federal de Lavras - UFLA, Avenida Dr. Sylvio Menicucci 1001, Caixa Postal 3037, 37200-000, Lavras, MG, Brazil. Electronic address: elaine.dorneles@dmv.ufla.br.
authors.forename: Elaine Maria Seles
authors.initials: EM
authors.lastname: Dorneles
authors.affiliation: Laboratório de Bacteriologia Aplicada, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Avenida Antônio Carlos 6627, Caixa Postal 567, 31270-901, Belo Horizonte, MG, Brazil. Electronic address: thiago.violacaipira@gmail.com.
authors.forename: Thiago Magalhães
authors.initials: TM
authors.lastname: Resende
authors.affiliation: Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina - UDESC, Avenida Luís de Camões 2090, 88520-000, Lages, SC, Brazil. Electronic address: adilvaz@gmail.com.
authors.forename: Adil Knackfuss
authors.initials: AK
authors.lastname: Vaz
authors.affiliation: Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina - UDESC, Avenida Luís de Camões 2090, 88520-000, Lages, SC, Brazil. Electronic address: sandra@cav.udesc.br.
authors.forename: Sandra Maria
authors.initials: SM
authors.lastname: Ferraz
authors.affiliation: Laboratório de Bacteriologia Aplicada, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Avenida Antônio Carlos 6627, Caixa Postal 567, 31270-901, Belo Horizonte, MG, Brazil. Electronic address: alage@vet.ufmg.br.
authors.forename: Andrey Pereira
authors.initials: AP
authors.lastname: Lage
mesh_terms: D000818:Animals; D002004:Brucella Vaccine / Q000008:administration & dosage*; D002003:Brucella abortus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control* / Q000821:virology; D003612:Dairying; D005260:Female; D008892:Milk / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary
chemical_list: D002004:Brucella Vaccine
keywords: Brucellosis; Cattle; Milk; RB51; Vaccination; Vaccine