title: TGF-β3-induced miR-494 inhibits macrophage polarization via suppressing PGE2 secretion in mesenchymal stem cells.
abstract: Abnormal macrophage polarization at the maternal-fetal interface may contribute to the development of Preeclampsia (PE). The reason why macrophage polarization changed in PE is still unclear. Decidual mesenchymal stem cells (dMSCs) could regulate macrophage polarization. However, miRNA in dMSCs of PE were maladjusted. Therefore, we speculated that miRNA may affect dMSC-regulated macrophage polarization. In this study, we found that miR-494-overexpressed dMSCs inhibit M2 macrophage polarization and this inhibitory effect is mediated by miR-494-reduced PGE2 secretion. Furthermore, we proved that miR-494 is induced by TGF-β3. In summary, our findings suggest that the high expression of TGF-β3 in PE decidua stimulates miR-494 in dMSCs and attenuates the regulation of MSC switching the macrophage toward M2 type, contributing to an immune imbalance at maternal-fetal interface.
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Zhao
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, China.
authors.forename: Huishuang
authors.initials: H
authors.lastname: Miao
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, China.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, China.|Jiangsu Key Laboratory of Molecular Medicine, Nanjing, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, China.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, China.|Jiangsu Key Laboratory of Molecular Medicine, Nanjing, China.
authors.forename: Yayi
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000328:Adult; D016764:Cell Polarity / Q000235:genetics*; D002478:Cells, Cultured; D003656:Decidua / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008262:Macrophage Activation / Q000235:genetics; D008264:Macrophages / Q000502:physiology*; D033261:Maternal-Fetal Relations; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D011225:Pre-Eclampsia / Q000276:immunology / Q000473:pathology; D011247:Pregnancy; D053782:Transforming Growth Factor beta3 / Q000235:genetics / Q000502:physiology*; D055815:Young Adult
chemical_list: C547168:MIRN494 microRNA, human; D035683:MicroRNAs; D053782:Transforming Growth Factor beta3; D015232:Dinoprostone
keywords: TGF-β3; macrophage polarization; mesenchymal stem cell; miR-494; preeclampsia