title: Characterization of a Novel Polerovirus Infecting Maize in China.
abstract: A novel virus, tentatively named Maize Yellow Mosaic Virus (MaYMV), was identified from the field-grown maize plants showing yellow mosaic symptoms on the leaves collected from the Yunnan Province of China by the deep sequencing of small RNAs. The complete 5642 nucleotide (nt)-long genome of the MaYMV shared the highest nucleotide sequence identity (73%) to Maize Yellow Dwarf Virus-RMV. Sequence comparisons and phylogenetic analyses suggested that MaYMV represents a new member of the genus Polerovirus in the family Luteoviridae. Furthermore, the P0 protein encoded by MaYMV was demonstrated to inhibit both local and systemic RNA silencing by co-infiltration assays using transgenic Nicotiana benthamiana line 16c carrying the GFP reporter gene, which further supported the identification of a new polerovirus. The biologically-active cDNA clone of MaYMV was generated by inserting the full-length cDNA of MaYMV into the binary vector pCB301. RT-PCR and Northern blot analyses showed that this clone was systemically infectious upon agro-inoculation into N. benthamiana. Subsequently, 13 different isolates of MaYMV from field-grown maize plants in different geographical locations of Yunnan and Guizhou provinces of China were sequenced. Analyses of their molecular variation indicate that the 3' half of P3-P5 read-through protein coding region was the most variable, whereas the coat protein- (CP-) and movement protein- (MP-)coding regions were the most conserved.
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. sophiachen920@126.com.
authors.forename: Sha
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. jgzzero@163.com.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. jgzzero@163.com.
authors.forename: Guangzhuang
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. wujx@zju.edu.cn.
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China. haoasliu@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. yjqian@zju.edu.cn.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. zzhou@zju.edu.cn.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. zzhou@zju.edu.cn.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002681:China; D003001:Cloning, Molecular; D016000:Cluster Analysis; D020868:Gene Silencing; D014644:Genetic Variation; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D052679:Luteoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D059386:Reverse Genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D003313:Zea mays / Q000821:virology*
keywords: Maize yellow mosaic virus; deep sequencing; infectious clone; molecular variation; sRNA; suppressor