title: Histochemical analysis of collagen fibers in giant cell fibroma and inflammatory fibrous hyperplasia.
abstract: OBJECTIVE
  The aim was to investigate collagen fibers in giant cell fibroma, inflammatory fibrous hyperplasia, and oral normal mucosa.
  
  
  METHODS
  Sixty-six cases were stained with picrosirius red. The slides were observed under polarization, followed by the measurement of the area and the percentage of the type I and type III collagens. The age and gender were obtained from the clinical records.
  
  
  RESULTS
  No differences could be observed in both the area and percentage of the type I and type III collagens within the categories of lesions and normal mucosa. In the giant cells fibroma, a greater area and percentage of type I collagen could be identified in individuals of less than 41.5 years (p<0.05).
  
  
  CONCLUSIONS
  The distribution of type I and type III collagen fibers in the studied lesions followed a similar pattern to that observed in the normal mucosa, indicating a normal collagen maturation process of type III to I. The study supports that multinucleated and stellate cells of the giant cell fibroma appear to be functional within collagen types III and I turnover. The greater amount of type I collagen identified in giant cell fibroma in individuals of less than 41.5 years reinforce the neoplastic nature of lesion.
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Mônica Jarema
authors.initials: MJ
authors.lastname: Schmidt
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Tschoeke
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Lúcia
authors.initials: L
authors.lastname: Noronha
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Rafaela Scariot de
authors.initials: RS
authors.lastname: Moraes
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6667 sala 3202-D, Pampulha, 31.270-901, Belo Horizonte, MG, Brazil.
authors.forename: Ricardo Alves
authors.initials: RA
authors.lastname: Mesquita
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Ana Maria Trindade
authors.initials: AM
authors.lastname: Grégio
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Luciana Reis Azevedo
authors.initials: LR
authors.lastname: Alanis
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Sérgio Aparecido
authors.initials: SA
authors.lastname: Ignácio
authors.affiliation: Department of Oral Pathology, School of Dentistry, Universidade Federal da Bahia, Av. Araújo Pinho, 62, Canela, 40110-150, Salvador, BA, Brazil.
authors.forename: Jean Nunes Dos
authors.initials: JN
authors.lastname: Santos
authors.affiliation: Department of Stomatology, School of Dentistry, Universidade Federal do Paraná, Rua Prefeito Lothário Meissner 632 Campus II, Jardim Botânico, 80210170 - Curitiba, PR, Brazil.
authors.forename: Antonio Adilson Soares de
authors.initials: AA
authors.lastname: Lima
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Teixeira Suelen
authors.initials: TS
authors.lastname: Luiz
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil.
authors.forename: Arielli Carine
authors.initials: AC
authors.lastname: Michels
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6667 sala 3202-D, Pampulha, 31.270-901, Belo Horizonte, MG, Brazil.
authors.forename: Maria Cássia Ferreira
authors.initials: MC
authors.lastname: Aguiar
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: alinecristinabatista@yahoo.com.br.
authors.forename: Aline Cristina Batista Rodrigues
authors.initials: AC
authors.lastname: Johann
mesh_terms: D000328:Adult; D005260:Female; D024022:Fibrillar Collagens / Q000378:metabolism*; D005350:Fibroma / Q000378:metabolism* / Q000473:pathology; D005870:Giant Cell Tumors / Q000378:metabolism* / Q000473:pathology; D015726:Giant Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D024022:Fibrillar Collagens
keywords: Collagen; Fibroma; Picrosirius red