title: Lipogenesis in myoblasts and its regulation of CTRP6 by AdipoR1/Erk/PPARγ signaling pathway.
abstract: The induced lipogenesis and its regulation in C2C12 myoblasts remain largely unclear. Here, we found that the cocktail method could significantly induce lipogenesis through regulating lipid metabolic genes and Erk1/2 phosphorylation in myoblasts. Meanwhile, the expression and secretion of CTRP6 were increased during ectopic lipogenesis. Moreover, CTRP6 knockdown down-regulated the levels of lipogenic genes and phosphorylated Erk1/2 (p-Erk1/2) in the early lipogenic stage, whereas up-regulated p-Erk1/2 in the terminal differentiation. Interestingly, the effect of CTRP6 siRNA was attenuated by U0126 (a special p-Erk1/2 inhibitor) in myoblasts. Furthermore, AdipoR1, not AdipoR2, was first identified as a receptor of CTRP6 during the process of mitotic clonal expansion. Collectively, we suggest that CTRP6 mediates the ectopic lipogenesis through AdipoR1/Erk/PPARγ signaling pathway in myoblasts. Our findings will shed light on the novel biological function of CTRP6 during myoblast lipogenesis and provide a hopeful direction of improving meat quality of domestic animal by lipogenic regulation in skeletal muscle myoblasts.
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wu
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Cunzhen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China pwj1226@nwsuaf.edu.cn gsyang999@hotmail.com.
authors.forename: Weijun
authors.initials: W
authors.lastname: Pang
authors.affiliation: Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China pwj1226@nwsuaf.edu.cn gsyang999@hotmail.com.
authors.forename: Gongshe
authors.initials: G
authors.lastname: Yang
mesh_terms: D054392:Adipokines / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D055785:Gene Knockdown Techniques; D050155:Lipogenesis / Q000235:genetics / Q000502:physiology*; D020935:MAP Kinase Signaling System; D051379:Mice; D008954:Models, Biological; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D054419:Receptors, Adiponectin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D054392:Adipokines; C000606745:CTRP6 protein, mouse; D047495:PPAR gamma; D034741:RNA, Small Interfering; D054419:Receptors, Adiponectin; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse
keywords: AdipoR1/Erk/PPARγ signaling pathway; C2C12 myoblast; CTRP6; lipogenesis