title: [The profile of plasma non-esterified fatty acids in children with different terms of type 1 diabetes mellitus].
abstract: Composition and quantitative content of non-esterified fatty acids (NEFA) were investigated in plasma samples of healthy children (12) and children with type 1 diabetes mellitus (DM1) (31) by gas chromatography (GC) after preliminary NEFA solid-phase extraction from plasma lipids. There was a significant (p<0.001) 1.6-fold increase in the total level of NEFA regardless of the disease duration. In the group of DM1 children with the disease period less than 1 year there was an increase in the arachidonic acid (20:4) content (30%) and the oleic acid trans-isomer (18:1) content (82%), and also a decrease in the docosahexaenoic acid (22:6 n3) content (26% ) and the docosapentaenoic acids (22:5 n-6) content (60%). In the group of DM1 children with prolonged course of this disease the altered NEFA levels returned to the normal level.
authors.affiliation: Department of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia; Institution of Biomedical Problems, Moscow, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Akmurzina
authors.affiliation: Morozov Children's Hospital, Moscow, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Petryairina
authors.affiliation: Research Institute of Human Morphology of the Russian Academy of Medical Sciences, Moscow, Russia; Institution of Biomedical Problems, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Saveliev
authors.affiliation: Department of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia; Institution of Biomedical Problems, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Selishcheva
mesh_terms: D016718:Arachidonic Acid / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D019301:Oleic Acid / Q000097:blood
chemical_list: D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D016718:Arachidonic Acid; D019301:Oleic Acid; C026219:docosapentaenoic acid
keywords: arachidonic acid; non-esterified fatty acids; solid-phase extraction; trans-fatty acid; type 1 diabetes