title: Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats.
abstract: An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm(2)) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Shakya
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Naveen
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Remya
authors.initials: R
authors.lastname: Vellachi
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Dayamon D
authors.initials: DD
authors.lastname: Mathew
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Prasoon
authors.initials: P
authors.lastname: Dubey
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Kiranjeet
authors.initials: K
authors.lastname: Singh
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Sonal
authors.initials: S
authors.lastname: Shrivastava
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Sameer
authors.initials: S
authors.lastname: Shrivastava
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Maiti
authors.affiliation: Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Anwarul
authors.initials: A
authors.lastname: Hasan
authors.affiliation: Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: K P
authors.initials: KP
authors.lastname: Singh