title: Growth inhibition and oxidative damage of Microcystis aeruginosa induced by crude extract of Sagittaria trifolia tubers.
abstract: Aquatic macrophytes are considered to be promising in controlling harmful cyanobacterial blooms. In this research, an aqueous extract of Sagittaria trifolia tubers was prepared to study its inhibitory effect on Microcystis aeruginosa in the laboratory. Several physiological indices of M. aeruginosa, in response to the environmental stress, were analyzed. Results showed that S. trifolia tuber aqueous extract significantly inhibited the growth of M. aeruginosa in a concentration-dependent way. The highest inhibition rate reached 90% after 6 day treatment. The Chlorophyll-a concentration of M. aeruginosa cells decreased from 343.1 to 314.2μg/L in the treatment group. The activities of superoxide dismutase and peroxidase and the content of reduced glutathione in M. aeruginosa cells initially increased as a response to the oxidative stress posed by S. trifolia tuber aqueous extract, but then decreased as time prolonged. The lipid peroxidation damage of the cyanobacterial cell membranes was reflected by the malondialdehyde level, which was notably higher in the treatment group compared with the controls. It was concluded that the oxidative damage of M. aeruginosa induced by S. trifolia tuber aqueous extract might be one of the mechanisms for the inhibitory effects.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China. Electronic address: lijiang1304@163.com.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China. Electronic address: liuyunguo204@163.com.
authors.forename: Yunguo
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Hunan East Dongting Lake National Nature Reserve Administration, Yueyang 414000, China.
authors.forename: Pingyang
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Cai
authors.affiliation: School of Architecture and Art, Central South University, Changsha 410082, China; School of Metallurgy and Environment, Central South University, Changsha 410083, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China.
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China.
authors.forename: Xinjiang
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Environmental Science and Engineering Research, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Tan
mesh_terms: D002734:Chlorophyll; D000077194:Chlorophyll A; D000458:Cyanobacteria; D005978:Glutathione / Q000378:metabolism; D057097:Harmful Algal Bloom / Q000187:drug effects*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D046931:Microcystis / Q000187:drug effects*; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D034903:Sagittaria; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D008315:Malondialdehyde; D010088:Oxidoreductases; D013482:Superoxide Dismutase; D005978:Glutathione; D000077194:Chlorophyll A
keywords: Antioxidant response; Cyanobacterial inhibition; Microcystis aeruginosa; Oxidative damage; Sagittaria trifolia