title: Benefits of Preventive Administration of Chlorella sp. on Visceral Pain and Cystitis Induced by a Single Administration of Cyclophosphamide in Female Wistar Rat.
abstract: Chlorella sp. is a green microalgae containing nutrients, vitamins, minerals, and chlorophyll. In some communities, Chlorella sp. is a traditional medicinal plant used for the management of inflammation-related diseases. In a rat model, ROQUETTE Chlorella sp. (RCs) benefits were investigated on visceral pain and associated inflammatory parameters related to cystitis both induced by cyclophosphamide (CYP). RCs was orally administered every day from day 1-16 (250 and 500 mg/kg body weight). Six hours after an intraperitoneal injection of 200 mg/kg body weight of CYP, body temperature, general behavior, food intake, and body weight were recorded. Twenty-four hours after CYP injection, rats were tested in two behavioral tests, an open field and the aversive light stimulus avoidance conditioning test, to evaluate the influence of pain on general activity and learning ability of rats. After euthanasia, bladders were weighed, their thickness was scored, and the urinary hemoglobin was measured. RCs orally administered at the two dosages significantly reduced visceral pain and associated inflammatory parameters related to cystitis both induced by CYP injection, and improved rat behavior. To conclude, RCs demonstrated beneficial effects against visceral pain and cystitis.
authors.affiliation: 1 ETAP-Lab, Parc Technologique de Nancy-Brabois , Vandœuvre-lès-Nancy, France .
authors.forename: Sophie
authors.initials: S
authors.lastname: Hidalgo-Lucas
authors.affiliation: 1 ETAP-Lab, Parc Technologique de Nancy-Brabois , Vandœuvre-lès-Nancy, France .
authors.forename: Pascale
authors.initials: P
authors.lastname: Rozan
authors.affiliation: 2 ROQUETTE , rue de la Haute Loge, Lestrem, France .
authors.forename: Laetitia
authors.initials: L
authors.lastname: Guérin-Deremaux
authors.affiliation: 2 ROQUETTE , rue de la Haute Loge, Lestrem, France .
authors.forename: Blandine
authors.initials: B
authors.lastname: Baert
authors.affiliation: 1 ETAP-Lab, Parc Technologique de Nancy-Brabois , Vandœuvre-lès-Nancy, France .
authors.forename: Nicolas
authors.initials: N
authors.lastname: Violle
authors.affiliation: 2 ROQUETTE , rue de la Haute Loge, Lestrem, France .
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Saniez-Degrave
authors.affiliation: 1 ETAP-Lab, Parc Technologique de Nancy-Brabois , Vandœuvre-lès-Nancy, France .
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Bisson
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000008:administration & dosage*; D001835:Body Weight / Q000187:drug effects; D002708:Chlorella / Q000737:chemistry*; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000009:adverse effects; D003556:Cystitis / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D004195:Disease Models, Animal; D004435:Eating / Q000187:drug effects; D005260:Female; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D059265:Visceral Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D001685:Biological Factors; D003520:Cyclophosphamide
keywords: Chlorella sp.; behavior; cystitis induced by a chemotherapeutic agent; microalgae; oral administration; pain; rodent