title: Rapid Detection of the GSTM3 A/B Polymorphism Using Real-time PCR with TaqMan(®) Probes.
abstract: Glutathione S-transferases (GSTs) are a group of phase II detoxification enzymes, which catalyze the conjugation of glutathione (GSH) with carcinogens, among other xenobiotics. The GSTM3 gene is part of the GSTs gene family, and its polymorphism A/B has been associated with risk and protective effects of several cancers. This genetic variant is a deletion of 3 bp (AGG) in intron 6. Previous association studies have performed genotyping using techniques such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In this study, we took advantage of the TaqMan(®) probes features and developed a reliable, faster, more simple and economic method to identify the 3-bp deletion. Our allelic discrimination method was able to distinguish between homozygous A/A, heterozygous A/B and homozygous B/B samples, as shown by TaqMan(®) based real-time PCR. Results were validated by Sanger Sequencing. In conclusion, we developed a specific and rapid method to detect the 3-bp deletion from the GSTM3 A/B polymorphism.
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, División Ciencias de la Salud, San Pedro Garza García, Nuevo León, México.
authors.forename: Denisse A
authors.initials: DA
authors.lastname: Martínez-Treviño
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, División Ciencias de la Salud, San Pedro Garza García, Nuevo León, México.
authors.forename: María G
authors.initials: MG
authors.lastname: Moreno-Treviño
authors.affiliation: Facultad de Medicina, Unidad Saltillo, Universidad Autónoma de Coahuila, Saltillo, Coahuila, México.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Salinas-Santander
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, División Ciencias de la Salud, San Pedro Garza García, Nuevo León, México.
authors.forename: Luisa
authors.initials: L
authors.lastname: Wohn
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, División Ciencias de la Salud, San Pedro Garza García, Nuevo León, México.
authors.forename: Sarahí
authors.initials: S
authors.lastname: Herrera-González
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, División Ciencias de la Salud, San Pedro Garza García, Nuevo León, México.
authors.forename: Marcelino
authors.initials: M
authors.lastname: Aguirre-Garza
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, División Ciencias de la Salud, San Pedro Garza García, Nuevo León, México.
authors.forename: O Carolina
authors.initials: OC
authors.lastname: Rojas
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, División Ciencias de la Salud, San Pedro Garza García, Nuevo León, México. Electronic address: rafael.reyesleon@udem.edu.
authors.forename: Rafael B R
authors.initials: RB
authors.lastname: León-Cachón
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D015342:DNA Probes; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007438:Introns; D007527:Isoenzymes / Q000235:genetics; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction; D017384:Sequence Deletion
chemical_list: D015342:DNA Probes; D007527:Isoenzymes; D005982:Glutathione Transferase; C416969:glutathione transferase M3-3
keywords: Glutathione S-transferases; PCR-RFLP; Polymorphism; Real-time PCR; TaqMan(®) probes