title: Phyto-mediated nanostructured carriers based on dual vegetable actives involved in the prevention of cellular damage.
abstract: The growing scientific interest in exploitation of vegetable bioactives has raised a number of questions regarding their imminent presence in pharmaceutical formulations. This study intends to demonstrate that a dual combination between vegetable oil (e.g. thistle oil, safflower oil, sea buckthorn oil) and a carrot extract represents an optimal approach to formulate safe carrier systems that manifest cell regeneration effect and promising antioxidant and anti-inflammatory activity. Inclusion of both natural actives into lipid carriers imparted a strong negative charge on the nanocarrier surface (up to -45mV) and displayed average sizes of 70nm to 140nm. The entrapment efficiency of carrot extract into nanostructured carriers ranged between 78.3 and 88.3%. The in vitro release study has demonstrated that the entrapment of the extract represents a viable way for an equilibrated release of carotenoids. Besides the excellent antioxidant properties (e.g. scavenging up to 98% of the free oxygen radicals), the results of cellular integrity (e.g. cell viability of 133%) recommend these nanocarriers based on dual carrot extract-bioactive oil as a promising trend for the treatment of certain disorders in which oxidative stress plays a prominent role. In addition, the lipid nanocarriers based on safflower oil and sea buckthorn oil demonstrated an anti-inflammatory effect on LPS induced THP-1 macrophages, by inhibiting the secretion of two pro-inflammatory cytokines, IL-6 and TNF-α.
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Istrati
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania.
authors.forename: I
authors.initials: I
authors.lastname: Lacatusu
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania.
authors.forename: N
authors.initials: N
authors.lastname: Bordei
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania.
authors.forename: G
authors.initials: G
authors.lastname: Badea
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania.
authors.forename: O
authors.initials: O
authors.lastname: Oprea
authors.affiliation: National Institute of Research and Development for Biological Sciences, Splaiul Independentei Street No. 296, 060031 Bucharest, Romania.
authors.forename: L M
authors.initials: LM
authors.lastname: Stefan
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania.
authors.forename: R
authors.initials: R
authors.lastname: Stan
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania. Electronic address: nicoleta.badea@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Badea
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Polizu Street No. 1, 011061 Bucharest, Romania.
authors.forename: A
authors.initials: A
authors.lastname: Meghea
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D018552:Daucus carota / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D031281:Hippophae / Q000737:chemistry*; D006801:Humans; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D012450:Safflower Oil / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004337:Drug Carriers; D010936:Plant Extracts; D012450:Safflower Oil
keywords: Carrot extract; Cell regenerative effect; In vitro antioxidant activity; Nanostructured carriers; Safflower oil