title: Detection of five potentially periodontal pathogenic bacteria in peri-implant disease: A comparison of PCR and real-time PCR.
abstract: The aim of this study was to compare the microbial analysis methods of polymerase chain reaction (PCR) and real-time PCR (RT-PCR) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. Therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using PCR (DNA-strip technology) and RT-PCR (fluorescent dye SYBR green-system): Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Treponema denticola (Td), Tanerella forsythia (Tf), and Fusobacterium nucleatum (Fn). There were no significant correlations between the bacterial and disease patterns, so the benefit of using microbiological tests for the diagnosis of peri-implant diseases is questionable. Correlations between the methods were highest for Tf (Kendall's Tau: 0.65, Spearman: 0.78), Fn (0.49, 0.61) and Td (0.49, 0.59). For Aa (0.38, 0.42) and Pg (0.04, 0.04), lower correlation values were detected. Accordingly, conventional semi-quantitative PCR seems to be sufficient for analyzing potentially periodontal pathogenic bacterial species.
authors.affiliation: Dept. of Cariology, Endodontology and Periodontology, University of Leipzig, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schmalz
authors.affiliation: Dept. of Preventive Dentistry, Periodontology and Cariology, University Medical Centre Goettingen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Tsigaras
authors.affiliation: Dental Practice Hanau & Alzenau, Germany; Dept. of Prosthodontics, University Medical Centre Goettingen, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Rinke
authors.affiliation: Clinical Research Organisation, Hamm, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Kottmann
authors.affiliation: Dept. of Cariology, Endodontology and Periodontology, University of Leipzig, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Haak
authors.affiliation: Dept. of Cariology, Endodontology and Periodontology, University of Leipzig, Germany. Electronic address: dirk.ziebolz@medizin.uni-leipzig.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Ziebolz
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D015921:Dental Implants; D006801:Humans; D057873:Peri-Implantitis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013280:Stomatitis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D015921:Dental Implants
keywords: Microbiological findings; PCR; Peri-implant mucositis; Peri-implantitis; Real-time PCR