title: [Regulation of SIRT1 by SREBP-1 in Adipogenic Differentiation of Mesenchymal Stem Cells].
abstract: OBJECTIVE
  To study the regulation of SIRT1 by transcription factor SREBP-1 in adipogeneic differentiation of bone marrow mesenchymal stem cells (BMMSC).
  
  
  METHODS
  Oil red O staining was used to identify the adipogenic differentiation of BMMSC; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 gene were detected by RT-PCR; the expession level of SREBP-1 was determined by Western-blot. The chromatin immunoprecipitation (ChIP) assay was used to investigate the binding of SREBP-1 to SIRT1 promoter.
  
  
  RESULTS
  BMMSC exposed to adipogenesis inducing medium become mature adipocytes at day 14; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 genes were up-regulated in adipocyte differentiation of BMMSC; the protein level of SREBP-1 was higher obviously; SIRT1 gene sequences was succesfully amplified from the genomic DNA immunoprecipitated by SREBP-1 antibody.
  
  
  CONCLUSIONS
  SREBP-1 can bind to the promoter region of the SIRT1 gene in adipogenesis of BMMSC, and may be involved in the transcriptional regulation of the SIRT1 gene.
authors.affiliation: Basic Medical College, Jiujiang University, Jiujiang, 332000, Jiangxi Province, China.|Medicine Graduate School, Nanchang University, Nanchang 330006, Jiangxi Province, China.|Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Hu
authors.affiliation: Basic Medical College, Jiujiang University, Jiujiang, 332000, Jiangxi Province, China. E-mail: caojun002@aliyun.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: Basic Medical College, Jiujiang University, Jiujiang, 332000, Jiangxi Province, China.
authors.forename: Chang-Chang
authors.initials: CC
authors.lastname: Yin
authors.affiliation: Medicine Graduate School, Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Wu
authors.affiliation: Basic Medical College, Jiujiang University, Jiujiang, 332000, Jiangxi Province, China.|Medicine Graduate School, Nanchang University, Nanchang 330006, Jiangxi Province, China.|Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.affiliation: Basic Medical College, Jiujiang University, Jiujiang, 332000, Jiangxi Province, China.|Medicine Graduate School, Nanchang University, Nanchang 330006, Jiangxi Province, China.|Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Zi-Yao
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011401:Promoter Regions, Genetic; D056564:Sirtuin 1 / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1; C447939:SIRT1 protein, human; D056564:Sirtuin 1