title: Preparation of Ti(4+)-immobilized modified silica capillary trapping column for on-line selective enrichment of phosphopeptides.
abstract: In this work, we have developed Ti(4+)-immobilized capillary trapping column (250μm i.d.) for highly specific on-line enrichment of phosphopeptides in the bio-samples. It minimizes the sample loss and improves the effectiveness of phosphopeptide detection that the limit of detection is as low as 1fmol/μL. It is successfully applied to the detection of phosphopeptides from complex biological samples, such as skim milk, human serum and mouse brain. The results indicate that the Ti(4+)-immobilized capillary trapping column is time-effective and has the great potential of application in low-abundance phosphopeptides on-line analysis. The prepared Ti(4+)-immobilized capillary trapping column will be further used in LC/MS platform for phosphoproteome analysis.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Huili
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433, People's Republic of China. Electronic address: chdeng@fudan.edu.cn.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.affiliation: Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433, People's Republic of China. Electronic address: xmzhang@fudan.edu.cn.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D006801:Humans; D009862:Online Systems; D010748:Phosphopeptides / Q000032:analysis*; D044967:Serum; D012822:Silicon Dioxide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium
chemical_list: D010748:Phosphopeptides; D012822:Silicon Dioxide; D014025:Titanium
keywords: On-line enrichment; Phosphopeptides detection; Ti(4+)-immobilized capillary trapping column