title: Optimization and microbial community analysis for production of biogas from solid waste residues of palm oil mill industry by solid-state anaerobic digestion.
abstract: This study investigated the improvement of biogas production from solid-state anaerobic digestion (SS-AD) of oil palm biomass by optimizing of total solids (TS) contents, feedstock to inoculum (F:I) ratios and carbon to nitrogen (C:N) ratios. Highest methane yield from EFB, OPF and OPT of 358, 280 and 324m(3)CH4ton(-1)VS, respectively, was achieved at TS content of 16%, C:N ratio of 30:1 and F:I ratio of 2:1. The main contribution to methane from biomass was the degradation of cellulose and hemicellulose. The highest methane production of 72m(3)CH4ton(-1) biomass was achieved from EFB. Bacteria community structure in SS-AD process of oil palm biomass was dominated by Ruminococcus sp. and Clostridium sp., while archaea community was dominated by Methanoculleus sp. Oil palm biomass has great potential for methane production via SS-AD.
authors.affiliation: Biotechnology Program, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand.
authors.forename: Wantanasak
authors.initials: W
authors.lastname: Suksong
authors.affiliation: Chemistry Division, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand.
authors.forename: Prawit
authors.initials: P
authors.lastname: Kongjan
authors.affiliation: Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Poonsuk
authors.initials: P
authors.lastname: Prasertsan
authors.affiliation: Division of Environmental Science and Engineering, Graduated school of Science and Engineering, Yamaguchi University, Yamaguchi 755-8611, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Imai
authors.affiliation: Biotechnology Program, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand; Research Center in Energy and Environment, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand. Electronic address: sompong.o@gmail.com.
authors.forename: Sompong
authors.initials: S
authors.lastname: O-Thong
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D008697:Methane / Q000096:biosynthesis; D009584:Nitrogen / Q000032:analysis; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D012037:Refuse Disposal / Q000379:methods*; D062611:Solid Waste / Q000032:analysis*
chemical_list: D056804:Biofuels; D010938:Plant Oils; D062611:Solid Waste; D000073878:Palm Oil; D009584:Nitrogen; D008697:Methane
keywords: Biogas production; Empty fruit bunches; Microbial community analysis; Oil palm biomass; Solid-state anaerobic digestion