title: Effect of thermal pretreatment on the biogas production and microbial communities balance during anaerobic digestion of urban and industrial waste activated sludge.
abstract: The effect of thermal pre-treatment on the microbial populations balance and biogas production was studied during anaerobic digestion of waste activated sludge (WAS) coming from urban (US: urban sludge) and industrial (IS: industrial sludge) wastewater treatment plants (WWTP). The highest biogas yields of 0.42l/gvolatile solid (VS) removed and 0.37l/gVS removed were obtained with urban and industrial sludge pre-treated at 120°C, respectively. Fluorescent in situ hybridization (FISH) was used to quantify the major Bacteria and Archaea groups. Compared to control trails without pretreatment, Archaea content increased from 34% to 86% and from 46% to 83% for pretreated IS and US, respectively. In fact, the thermal pre-treatment of WAS enhanced the growth of hydrogen-using methanogens (HUMs), which consume rapidly the H2 generated to allow the acetogenesis. Therefore, the stable and better performance of digesters was observed involving the balance and syntrophic associations between the different microbial populations.
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia; Faculty of Marine and Environmental Sciences, University of Cádiz, Pol. Río San Pedro s/n, 11510 Puerto Real (Cádiz), Spain.
authors.forename: Hajer
authors.initials: H
authors.lastname: Ennouri
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia.
authors.forename: Baligh
authors.initials: B
authors.lastname: Miladi
authors.affiliation: Faculty of Marine and Environmental Sciences, University of Cádiz, Pol. Río San Pedro s/n, 11510 Puerto Real (Cádiz), Spain.
authors.forename: Soraya Zahedi
authors.initials: SZ
authors.lastname: Diaz
authors.affiliation: Faculty of Sciences, University of Cádiz, Pol. Río San Pedro s/n, 11510 Puerto Real (Cádiz), Spain.
authors.forename: Luis Alberto Fernández
authors.initials: LAF
authors.lastname: Güelfo
authors.affiliation: Faculty of Marine and Environmental Sciences, University of Cádiz, Pol. Río San Pedro s/n, 11510 Puerto Real (Cádiz), Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Solera
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Université de Carthage, LR: LETMi, INSAT, B.P. 676, 1080 Tunis, Tunisia. Electronic address: hassibbouallagui@yahoo.fr.
authors.forename: Hassib
authors.initials: H
authors.lastname: Bouallagui
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels / Q000032:analysis / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D007220:Industrial Waste; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D056804:Biofuels; D007220:Industrial Waste; D012722:Sewage; D062065:Wastewater
keywords: Anaerobic digestion; Fluorescent in situ hybridization; Industrial sludge; Microbial balance; Thermal pre-treatment; Urban sludge