title: Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats.
abstract: The pandemic tendency of obesity and its strong association with serious co-morbidities have elicited interest in the underlying mechanisms of these pathologies. Lipid homeostasis, closely involved in obesity, has been reported to be regulated by multiple pathways. mTORC1 is emerging as a critical regulator of lipid metabolism. Here, we describe that the consumption of soy isoflavones, with a structural similarity to that of estradiol, could mitigate obesity through an AKT/mTORC1 pathway. Fed with soy isoflavones, the diet-induced obesity (DIO) male rats exhibited decreased body weight, accompanied with suppressed lipogenesis and adipogenesis, as well as enhanced lipolysis and β‑oxidation. The phosphorylation of AKT and S6 were decreased after soy isoflavone treatment in vivo and in vitro, suggesting an inhibition effect of soy isoflavones on mTORC1 activity. Our study reveals a potential mechanism of soy isoflavones regulating lipid homeostasis, which will be important for obesity treatment.
authors.affiliation: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. huangchao@sicau.edu.cn.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. huangchao@sicau.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. pangdejiang@gmail.com.
authors.forename: Dejiang
authors.initials: D
authors.lastname: Pang
authors.affiliation: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. lqhbiology@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. lqhbiology@163.com.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. chenxl3984@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. chenxl3984@163.com.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. gaoqi12@126.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. gaoqi12@126.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. shiliangqin87@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. shiliangqin87@163.com.
authors.forename: Liangqin
authors.initials: L
authors.lastname: Shi
authors.affiliation: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. liuwt1986@126.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. liuwt1986@126.com.
authors.forename: Wentao
authors.initials: W
authors.lastname: Liu
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. yuanfengzou@sicau.edu.cn.
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. lilixia905@163.com.
authors.forename: Lixia
authors.initials: L
authors.lastname: Li
authors.affiliation: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. chzhli75@163.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. chzhli75@163.com.
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Chen
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D007529:Isoflavones / Q000008:administration & dosage* / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D000076222:Mechanistic Target of Rapamycin Complex 1; D046912:Multiprotein Complexes / Q000378:metabolism*; D009765:Obesity / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013025:Glycine max / Q000737:chemistry*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D007529:Isoflavones; D046912:Multiprotein Complexes; D010936:Plant Extracts; D019301:Oleic Acid; D000076222:Mechanistic Target of Rapamycin Complex 1; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases
keywords: lipid metabolism; mTORC1; obesity; soy isoflavones