title: Case-Control Study on Impact of the Telomerase Reverse Transcriptase Gene Polymorphism and Additional Single Nucleotide Polymorphism (SNP)- SNP Interaction on Non-Small Cell Lung Cancers Risk in Chinese Han Population.
abstract: OBJECTIVE
  To investigate the impact of telomerase reverse transcriptase (TERT) gene polymorphism and additional SNP-SNP interaction on non-small cell lung cancer (NSCLC) risk in Chinese population.
  
  
  METHODS
  A total of 828 participants (526 males, 302 females), with a mean age of 71.3 ± 15.7 years old, were selected, including 410 NSCLC patients and 418 normal participants. Logistic regression was performed to investigate association between single nucleotide polymorphism (SNP) and NSCLC risk. Generalized multifactor dimensionality reduction (GMDR) was used to analysis the interaction among four SNPs.
  
  
  RESULTS
  Non-small cell lung cancer risk was significantly higher in carriers of G allele of the rs2736100 polymorphism than those with TT (TG + GG vs. TT, adjusted OR (95%CI = 1.68 (1.28-2.07). In addition, we also found that NSCLC risk was also significantly higher in carriers of A allele of the rs2736098 polymorphism than those with GG (GA + AA vs. GG, adjusted OR (95%CI) = 1.52 (1.19-1.93). GMDR analysis indicated that there was a significant two-locus model (P = 0.0100) involving rs2736098 and rs2736100, indicating a potential gene-gene interaction between rs2736098 and rs2736100. Overall, the two-locus models had a cross-validation consistency of 10 of 10, and had the testing accuracy of 62.17%. We found that patients with GA or AA of rs2736098 and TG or GG of rs2736100 genotype have the highest NSCLC risk, compared to patients with GG of rs2736098 and TT of rs2736100 genotype, OR (95%CI) was 2.52 (1.68-3.68), after covariates adjustment.
  
  
  CONCLUSIONS
  Minor allele of rs2736098 and rs2736100 in TERT gene and interaction between the two SNP were associated with increased risk of NSCLC risk.
authors.affiliation: Department of Internal Medicine, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Xing
authors.affiliation: Department of Senile Disease, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jian-Cong
authors.initials: JC
authors.lastname: Lin
authors.affiliation: Department of Senile Disease, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Zhu
authors.affiliation: Department of Internal Medicine, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Chang-Ran
authors.initials: CR
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. niummxp216@163.com.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Niu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012189:Retrospective Studies; D012307:Risk Factors; D019098:Telomerase / Q000235:genetics*
chemical_list: D019098:Telomerase
keywords: interaction; non-small cell lung cancer; polymorphism; single nucleotide polymorphism; telomerase reverse transcriptase