title: Fabrication and biocompatibility of poly(l-lactic acid) and chitosan composite scaffolds with hierarchical microstructures.
abstract: The scaffold microstructure is crucial to reconstruct tissue normal functions. In this article, poly(l-lactic acid) and chitosan fiber (PLLA/CTSF) composite scaffolds with hierarchical microstructures both in fiber and pore sizes were successfully fabricated by combining thermal induced phase separation and salt leaching techniques. The composite scaffolds consisted of a nanofibrous PLLA matrix with diameter of 50-500nm, and chitosan fibers with diameter of about 20μm were homogenously distributed in the PLLA matrix as a microsized reinforcer. The composite scaffolds also had high porosity (>94%) and hierarchical pore size, which were consisted of both micropores (50nm-10μm) and macropores (50-300μm). By tailoring the microstructure and chemical composition, the mechanical property, pH buffer and protein adsorption capacity of the composite scaffold were improved significantly compared with those of PLLA scaffold. Cell culture results also revealed that the PLLA/CTSF composite scaffolds supported MG-63 osteoblast proliferation and penetration.
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China. Electronic address: taolou72@aliyun.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Lou
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Physics & Collaborative Innovation Center for Low-Dimensional Nanomaterials and Optoelectronic Devices, Qingdao University, Qingdao 266071, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Miao
authors.affiliation: College of Physics & Collaborative Innovation Center for Low-Dimensional Nanomaterials and Optoelectronic Devices, Qingdao University, Qingdao 266071, China. Electronic address: yunzelong@163.com.
authors.forename: Yun-Ze
authors.initials: YZ
authors.lastname: Long
authors.affiliation: Department of Osteology, No. 401 Hospital of P. L. A., Qingdao 266071, China.
authors.forename: Hai-Lei
authors.initials: HL
authors.lastname: Yin
authors.affiliation: College of Physics & Collaborative Innovation Center for Low-Dimensional Nanomaterials and Optoelectronic Devices, Qingdao University, Qingdao 266071, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Song
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C033616:poly(lactide); D048271:Chitosan
keywords: Chitosan; Hierarchical; Microstructure; PLLA; Scaffold