title: [MiR-181a Promotes Proliferation of Human Acute Myeloid Leukemia Cells by Targeting ATM].
abstract: OBJECTIVE
  To investigate miR-181a function and regulation mechanism by identifying miR-181a target genes in acute myeloid leukemia (AML).
  
  
  METHODS
  The HL-60 cells of human AML was transfected by small molecular analog miR-181a, the cell proliferation was detected by CCK-8 method after electroporation in HL-60 cell lines. Target genes of miR-181a were predicted and analyzed by the bioinformatics software and database. Target genes were confirmed by HL-60 cell line and the patient leukemia cells.
  
  
  RESULTS
  Overexpressed miR-181a in HL-60 cell line significantly enhanced cell proliferation compared with that in control (P < 0.05). Dual luciferase reporter gene assay showed that miR-181a significantly suppressed the reporter gene activity containing ATM 3'-UTR by about 56.8% (P < 0.05), but it didn't suppress the reporter gene activity containing 3'-UTR ATM mutation. Western blot showed that miR-181a significantly downregulated the expression of ATM in human leukemia cells. It is also found that miR-181a was significantly increased in AML, which showed a negative correlation with ATM expression.
  
  
  CONCLUSIONS
  miR-181a promotes cell proliferation in AML by regulating the tumor suppressor ATM, thus it plays the role as oncogene in pathogenesis of AML.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, Guangdong Province, China. E-mail: jy1016aa@163.com.
authors.forename: Jia-Ye
authors.initials: JY
authors.lastname: Hua
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, Guangdong Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, Guangdong Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, Guangdong Province, China.
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guanghzou 510515, Guangdong Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Hematology Laboratory, Guangzhou Women and Children Medical Center, Guangzhou 510623, Guangdong Province, China.
authors.forename: Mu-Xia
authors.initials: MX
authors.lastname: Yan
mesh_terms: D064007:Ataxia Telangiectasia Mutated Proteins / Q000378:metabolism*; D049109:Cell Proliferation; D015536:Down-Regulation; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: C540193:MIrn181 microRNA, human; D035683:MicroRNAs; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins