title: The endothelial nitric oxide synthase (eNOS) polymorphism in otitis media with effusion (OME).
abstract: OBJECTIVE
  Otitis media with effusion (OME) is the most common disease after viral infections of upper respiratory tract (URTI) in children. Studies indicate the important role of nitric oxide (NO) in the etiology of hearing loss. However, there is no study that focuses on the role of nitric oxide synthase (eNOS) polymorphisms in the cases with OME. The aim of the present study is to evaluate the eNOS polymorphisms in the pediatric patients with OME.
  
  
  METHODS
  Eighty-nine patients who are diagnosed with otitis media with effusion and 85 healthy subjects who are compatible in terms of age and gender were included in the study. All patients in the study were subjected to complete ear, nose, throat (ENT) and audiological examinations. DNA analysis was performed with polymerase chain reaction (PCR) technique from the blood samples. The PCR product was cut by restriction fragment length polymorphism (RFLP) with BanII enzyme and checked by agarose gel electrophoresis.
  
  
  RESULTS
  As a result of genetic analysis, there is no significant difference between patients and the controls in terms of eNOS Glu298Asp polymorphism (G/G, G/T, T/T). When these groups were compared in terms of allele distributions, a significant relationship was found between the patients and the controls (P=0.037).
  
  
  CONCLUSIONS
  To the best of our knowledge, G allele was identified as predisposing to the development of OME and this is the first report indicates the correlation between the eNOS G894T polymorphism and OME in Turkey.
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Otorhinolaryngology, Hatay, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ates
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Otorhinolaryngology, Hatay, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Cevik
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Physiology, Hatay, Turkey; Mustafa Kemal University, School of Medicine, Department of Molecular Biochemistry and Genetics, Hatay, Turkey. Electronic address: drecepfatih@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Dokuyucu
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Otorhinolaryngology, Hatay, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Berber
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Otorhinolaryngology, Hatay, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Colak
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Medical Biology, Hatay, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Izmirli
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D010034:Otitis Media with Effusion / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014421:Turkey
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: Otitis media with effusion; Serous otitis; Turkey; eNOS polymorphism