title: Effects of acrylic resin monomers on porcine coronary artery reactivity.
abstract: The purpose of the present investigation was to assess the reactivity of porcine coronary arteries under in vitro conditions following their exposure to methyl methacrylate (MMA) and hydroxyethyl methacrylate (HEMA) monomers. Confirming previous studies using rat aortas, both MMA and HEMA induced acute/direct relaxation of coronary ring preparations, which was partly dependent on the endothelium. With prolonged tissue exposure, both monomers caused time- and concentration-dependent inhibition of receptor-mediated contraction of the vascular smooth muscle caused by prostaglandin F2∝ (PGF2∝), with HEMA causing more inhibition than MMA. Hydroxyethyl methacrylate, but not MMA, also produced impairment of non-receptor-mediated contraction of the coronary smooth muscle induced by KCl. On the other hand, neither HEMA nor MMA altered relaxation of the smooth muscle produced by the direct-acting pharmacological agent, sodium nitroprusside (SNP). While exposure to HEMA impaired endothelium-dependent vasorelaxation caused by bradykinin (BK), MMA markedly enhanced this endothelial-mediated response of the arteries. The enhanced endothelial response produced by MMA was linked to nitric oxide (NO) release. In conclusion, with prolonged tissue exposure, MMA causes less pronounced effects/adverse consequences on coronary smooth muscle function relative to the effect of HEMA, while enhancing vasorelaxation associated with release of NO from the endothelium. Accordingly, MMA-containing resin materials appear to be safer for human applications than materials containing HEMA.
authors.affiliation: a Department of Oral Biology , Dental College of Georgia, Augusta University , Augusta , GA , USA.
authors.forename: Worku
authors.initials: W
authors.lastname: Abebe
authors.affiliation: b Periodontal Section , DentFirst Dental Care , Norcross , GA , USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: West
authors.affiliation: c Section of Dental Material, Department of Oral Rehabilitation , Dental College of Georgia, Augusta University , Augusta , GA , USA.
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Rueggeberg
authors.affiliation: a Department of Oral Biology , Dental College of Georgia, Augusta University , Augusta , GA , USA.
authors.forename: David
authors.initials: D
authors.lastname: Pashley
authors.affiliation: a Department of Oral Biology , Dental College of Georgia, Augusta University , Augusta , GA , USA.
authors.forename: Mahmood S
authors.initials: MS
authors.lastname: Mozaffari
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D023903:Coronary Restenosis / Q000517:prevention & control; D003331:Coronary Vessels / Q000187:drug effects*; D015237:Dinoprost / Q000494:pharmacology; D004727:Endothelium / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008689:Methacrylates / Q000494:pharmacology; D020366:Methylmethacrylate / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009569:Nitric Oxide / Q000737:chemistry; D009599:Nitroprusside / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D013552:Swine; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D000180:Acrylic Resins; D008689:Methacrylates; D014665:Vasodilator Agents; D009599:Nitroprusside; D020366:Methylmethacrylate; D009569:Nitric Oxide; D011189:Potassium Chloride; C005044:hydroxyethyl methacrylate; D015237:Dinoprost; D001920:Bradykinin
keywords: HEMA; MMA; Resin monomers; methacrylate biocompatibility; vascular reactivity