title: Thiamine Deficiency Increases Ca2+Current and CaV1.2 L-type Ca2+Channel Levels in Cerebellum Granular Neurons.
abstract: Thiamine (vitamin B1) is co-factor for three pivotal enzymes for glycolytic metabolism: pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and transketolase. Thiamine deficiency leads to neurodegeneration of several brain regions, especially the cerebellum. In addition, several neurodegenerative diseases are associated with impairments of glycolytic metabolism, including Alzheimer's disease. Therefore, understanding the link between dysfunction of the glycolytic pathway and neuronal death will be an important step to comprehend the mechanism and progression of neuronal degeneration as well as the development of new treatment for neurodegenerative states. Here, using an in vitro model to study the effects of thiamine deficiency on cerebellum granule neurons, we show an increase in Ca2+current density and CaV1.2 expression. These results indicate a link between alterations in glycolytic metabolism and changes to Ca2+dynamics, two factors that have been implicated in neurodegeneration.
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Bloco K4, Sala #167, Belo Horizonte, MG, CEP 31270-901, Brazil.
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Moreira-Lobo
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Bloco K4, Sala #167, Belo Horizonte, MG, CEP 31270-901, Brazil. jcruz@icb.ufmg.br.
authors.forename: Jader S
authors.initials: JS
authors.lastname: Cruz
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Bloco K4, Sala #167, Belo Horizonte, MG, CEP 31270-901, Brazil.
authors.forename: Flavia R
authors.initials: FR
authors.lastname: Silva
authors.affiliation: Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Bloco K4, Sala #167, Belo Horizonte, MG, CEP 31270-901, Brazil.
authors.forename: Fabíola M
authors.initials: FM
authors.lastname: Ribeiro
authors.affiliation: Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31270-901, Brazil.
authors.forename: Christopher
authors.initials: C
authors.lastname: Kushmerick
authors.affiliation: Center for Mathematics, Computing and Cognition (CMCC), Universidade Federal do ABC - UFABC, Rua Arcturus, 03 - Jardim Antares, Bloco Delta; 2º Andar; Sala: 248, São Bernardo do Campo, SP, CEP 09606-070, Brazil. oliveira.fernando@ufabc.edu.br.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D002531:Cerebellum / Q000473:pathology*; D015151:Immunoblotting; D015640:Ion Channel Gating; D009474:Neurons / Q000378:metabolism*; D017208:Rats, Wistar; D012032:Refractory Period, Electrophysiological; D013832:Thiamine Deficiency / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D020746:Calcium Channels, L-Type; D002118:Calcium
keywords: Ca2+ current; Cerebellum; Granular neuron; Neurodegeneration; Thiamine