title: Identification of antigenic domains in the non-structural protein of Muscovy duck parvovirus.
abstract: Muscovy duck parvovirus (MDPV) infection is widespread in many Muscovy-duck-farming countries, leading to a huge economic loss. By means of overlapping peptides expressed in Escherichia coli in combination with Western blot, antigenic domains on the non-structural protein (NSP) of MDPV were identified for the first time. On the Western blot, the fragments NS(481-510), NS (501-530), NS (521-550), NS (541-570), NS (561-590), NS (581-610) and NS (601-627) were positive (the numbers in parentheses indicate the location of amino acids), and other fragments were negative. These seven fragments were also reactive in an indirect enzyme-linked immunosorbent assay (i-ELISA). We therefore conclude that a linear antigenic domain of the NSP is located at its C-terminal end (amino acid residues 481-627). These results may facilitate future investigations into the function of NSP of MDPV and the development of immunoassays for the diagnosis of MDPV infection.
authors.affiliation: College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar, People's Republic of China. yutianfei2001@163.com.
authors.forename: Tian-Fei
authors.identifier: http://orcid.org/0000-0003-4868-8142
authors.initials: TF
authors.lastname: Yu
authors.affiliation: College of Computer and Control Engineering, Qiqihar University, Qiqihar, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yan
authors.affiliation: College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar, People's Republic of China.
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Shao
authors.affiliation: Heilongjiang Institute of Veterinary Medicine Science, Qiqihar, People's Republic of China.
authors.forename: Xing-Dong
authors.initials: XD
authors.lastname: Fan
authors.affiliation: China Institute of Veterinary Drug Control, Beijing, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Veterinary Medicine Research Center, Beijing DaBeiNong Science and Technology Group Co., Ltd. (DBN), Beijing, People's Republic of China.
authors.forename: Dan-Na
authors.initials: DN
authors.lastname: Wang
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004372:Ducks; D018604:Epitope Mapping; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins
keywords: ELISA; Epitope; Muscovy duck parvovirus; Non-structural protein; Western blot