title: Physicochemical characterization of a navy bean (Phaseolus vulgaris) protein fraction produced using a solvent-free method.
abstract: A solvent-free electrostatic separation method was employed to separate navy bean flour (NBF) into protein-rich (PR) and starch-rich (SR) fractions. The physicochemical properties of NBF and separated fractions were compared to proteins (navy bean isolate (NBI) and 7S globulin) prepared using a wet process. Gel electrophoresis confirmed that the protein distribution in the isolated fractions was similar to that of NBF. The protein profile of NBI and 7S globulin was found to be devoid of certain proteins that were found in the NBF and PR fraction. Amino acid analysis revealed that the NBI and 7S globulin had a lower content of sulfur-containing amino acids compared to NBF and the electrostatically isolated fractions. CD and fluorescence spectroscopy confirmed that denaturation of the proteins during the acid precipitation is likely. This novel solvent-free electrostatic separation process preserves the native protein structure found in NBF and improves the recovery of some of the smaller MW proteins.
authors.affiliation: Department of Chemical Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada; Advanced CERT Canada Inc., 55 King Street West, Kitchener, ON N2G 4W1, Canada.
authors.forename: Mousa
authors.initials: M
authors.lastname: Jafari
authors.affiliation: Advanced CERT Canada Inc., 55 King Street West, Kitchener, ON N2G 4W1, Canada; School of Engineering Technology, McMaster University, Hamilton, ON L8S 0A3, Canada.
authors.forename: Amin Reza
authors.initials: AR
authors.lastname: Rajabzadeh
authors.affiliation: Department of Chemical Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
authors.forename: Solmaz
authors.initials: S
authors.lastname: Tabtabaei
authors.affiliation: Genomics and Biotechnology, London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marsolais
authors.affiliation: Department of Chemical Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada. Electronic address: rllegge@uwaterloo.ca.
authors.forename: Raymond L
authors.initials: RL
authors.lastname: Legge
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D005433:Flour / Q000032:analysis*; D005916:Globulins / Q000032:analysis*; D027805:Phaseolus / Q000737:chemistry*; D013213:Starch / Q000032:analysis*
chemical_list: D000596:Amino Acids; D005916:Globulins; D013213:Starch
keywords: Electrostatic separation; Isoelectric precipitation; Navy bean; Phaseolus vulgaris; Physicochemical characterization; Protein isolate; Solvent-free fractionation