title: Bio-scaffolds produced from irradiated squid pen and crab chitosan with hydroxyapatite/β-tricalcium phosphate for bone-tissue engineering.
abstract: In this study, bio-scaffolds have been developed using irradiated chitosan from different sources - squid pen (RS) and crab shell (RC) - with hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) at a chitosan/HA/β-TCP ratio of 50/30/20. The bio-scaffolds were prepared at two different freezing temperature (-20°C and -80°C) followed by lyophilisation. To enhance the mechanical properties, the bio-scaffolds were cross-linked using sodium tripolyphosphate (TPP) followed by lyophilisation. The composition and morphology of the bio-scaffolds were characterized using XRD, SEM, TEM and μ-CT. The pore size of the porous scaffolds ranged from 90 to 220μm and the scaffolds had 70-80% porosity. The scaffolds had a water uptake ratio of more than 10, and a controlled biodegradation in the range of 30-40%. These results suggest that the physical and biological properties of chitosan-based bio-scaffolds can be a promising biomaterial for bone-tissue regeneration.
authors.affiliation: Department of Food Sciences, University of Otago, Dunedin, New Zealand. Electronic address: amin.shavandi@postgrad.otago.ac.nz.
authors.forename: Amin
authors.initials: A
authors.lastname: Shavandi
authors.affiliation: Department of Food Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Alaa El-Din A
authors.initials: AE
authors.lastname: Bekhit
authors.affiliation: Department of Physics, University of Otago, Dunedin, New Zealand.
authors.forename: Zhifa
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Applied Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: M Azam
authors.initials: MA
authors.lastname: Ali
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D003386:Brachyura; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D049832:Decapodiformes; D006244:Hardness; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D010006:Osteoblasts / Q000502:physiology; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C485817:beta-tricalcium phosphate; C074950:hydroxyapatite-beta tricalcium phosphate; D048271:Chitosan
keywords: Hydroxyapatite; Irradiation; Squid pen chitosan