title: Proteometabolomic analysis of transgenic tomato overexpressing oxalate decarboxylase uncovers novel proteins potentially involved in defense mechanism against Sclerotinia.
abstract: Oxalic acid (OA) plays dual role in fungal pathogenicity in a concentration dependent manner. While at higher concentration it induces programmed cell death leading to fungal invasion, low oxalate build resistance in plant. Although OA has been identified as a virulence determinant for rot disease caused by Sclerotinia sp., our understanding of how oxalate downregulation impart host immunity is limited. We have earlier shown that ectopic expression of oxalate decarboxylase (FvOXDC) specifically degrades OA in tomato (Solanum lycopersicum). To elucidate low oxalate regulated molecular mechanism imparting immunity, a comparative proteomics approach has been applied to E8.2-OXDC tomato fruit displaying fungal resistance. Mass spectrometric analyses identified 92 OXDC-responsive immunity related protein spots (ORIRPs) presumably associated with acid metabolism, defense signaling and endoplasmic reticulum stress. Metabolome study indicated increased abundance of some of the organic acids paralleling the proteomic analysis. Further, we interrogated the proteome data using network analysis that identified modules enriched in known and novel immunity-related prognostic proteins centered around 14-3-3, translationally controlled tumor protein, annexin and chaperonin. Taken together, our data demonstrate that low oxalate may act as metabolic and immunity determinant through translational reprogramming.
  
  
  
  Although OA plays critical role as fungal elicitor, our understanding of how oxalate downregulation by decarboxylative degradation impart immunity is limited. Our study confirms the impact of oxalate down-regulation on overall cellular physiology and provides new perspectives to study plant immunity. The network representation may facilitate the prioritization of candidate proteins for patho-stress tolerance in crop plant. These findings are of great importance for future work towards functional determination and exploitation of target proteins in crop improvement program.
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Kanika
authors.initials: K
authors.lastname: Narula
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Arunima
authors.initials: A
authors.lastname: Sinha
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Rajgourab
authors.initials: R
authors.lastname: Ghosh
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Jawa
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India. Electronic address: subhrac@hotmail.com.
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
mesh_terms: D001203:Ascomycota / Q000187:drug effects / Q000276:immunology*; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D055432:Metabolomics; D019815:Oxalic Acid / Q000378:metabolism; D057865:Plant Immunity / Q000187:drug effects; D010940:Plant Proteins / Q000276:immunology; D030821:Plants, Genetically Modified / Q000276:immunology* / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000276:immunology; D040901:Proteomics; D019076:Transgenes
chemical_list: D010940:Plant Proteins; D020543:Proteome; D019815:Oxalic Acid; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase
keywords: Comparative proteomics and protein network; Immunity; Metabolomics; Oxalate decarboxylase; Oxalic acid; Tomato