title: Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations.
abstract: ♦ BACKGROUND: Permanent stimulation of the peritoneum during peritoneal dialysis (PD) is likely to result in increased expression of genes encoding proteins involved in inflammation and tissue remodeling. Peritoneal fibrosis and neoangiogenesis may develop. ♦ OBJECTIVE: To assess highly expressed genes potentially in volved in peritoneal alterations during PD treatment using an animal model. ♦ METHODS: A PD catheter was implanted in 36 male Wistar rats after 70% nephrectomy. The rats were divided into 3 groups, exposed to dialysis solution for 8 weeks, and sacrificed 2 weeks later. Group B was exposed to a buffer, group D was exposed to a 3.86% glucose-based dialysis solution, and in group D+H, a second hit of intraperitoneal blood on top of the dialysis solution was given to induce the development of peritoneal sclerosis. Before sacrifice, peritoneal function was assessed. Omental tissue was obtained for analysis of gene expression using RT-qPCR. ♦ RESULTS: Fibrosis scores, vessel counts, and peritoneal function parameters were not different between the groups. Genes involved in the transforming growth factor beta signaling pathway, cell proliferation, angiogenesis, and inflammation were more expressed (p < 0.05) in the D+H group. Almost no differences were found between the control groups. We identified 4 genes that were related to peritoneal transport. ♦ CONCLUSION: Already a mid-term peritoneal exposure, when no microscopical and functional alterations are present, provokes activation of gene pathways of cell proliferation, fibrosis, neoangiogenesis, and inflammation.
authors.affiliation: Department of Nephrology, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic alena.parikova@ikem.cz.
authors.forename: Alena
authors.initials: A
authors.lastname: Parikova
authors.affiliation: Division of Nephrology Department of Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Anniek
authors.initials: A
authors.lastname: Vlijm
authors.affiliation: Department of Transplant Laboratory, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Brabcova
authors.affiliation: Division of Nephrology Department of Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Marijke
authors.initials: M
authors.lastname: de Graaff
authors.affiliation: Division of Nephrology Department of Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Dirk G
authors.initials: DG
authors.lastname: Struijk
authors.affiliation: Department of Nephrology, Transplant Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Viklicky
authors.affiliation: Division of Nephrology Department of Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Krediet
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D016001:Confidence Intervals; D015314:Dialysis Solutions / Q000494:pharmacology; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007676:Kidney Failure, Chronic / Q000628:therapy*; D008297:Male; D009389:Neovascularization, Pathologic / Q000235:genetics*; D009392:Nephrectomy / Q000379:methods; D010530:Peritoneal Dialysis / Q000009:adverse effects / Q000379:methods*; D056627:Peritoneal Fibrosis / Q000235:genetics* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D015314:Dialysis Solutions
keywords: Peritoneal dialysis; angiogenesis; fibrosis; gene expression; inflammation; peritoneal membrane alterations; peritoneal transport