title: Rapid and Efficient Stable Gene Transfer to Mesenchymal Stromal Cells Using a Modified Foamy Virus Vector.
abstract: Mesenchymal stromal cells (MSCs) hold great promise for regenerative medicine. Stable ex vivo gene transfer to MSCs could improve the outcome and scope of MSC therapy, but current vectors require multiple rounds of transduction, involve genotoxic viral promoters and/or the addition of cytotoxic cationic polymers in order to achieve efficient transduction. We describe a self-inactivating foamy virus vector (FVV), incorporating the simian macaque foamy virus envelope and using physiological promoters, which efficiently transduces murine MSCs (mMSCs) in a single-round. High and sustained expression of the transgene, whether GFP or the lysosomal enzyme, arylsulphatase A (ARSA), was achieved. Defining MSC characteristics (surface marker expression and differentiation potential), as well as long-term engraftment and distribution in the murine brain following intracerebroventricular delivery, are unaffected by FVV transduction. Similarly, greater than 95% of human MSCs (hMSCs) were stably transduced using the same vector, facilitating human application. This work describes the best stable gene transfer vector available for mMSCs and hMSCs.
authors.affiliation: Jefferiss Research Trust laboratories, Department of Medicine, Imperial College London, London, UK.
authors.forename: Nathan Paul
authors.initials: NP
authors.lastname: Sweeney
authors.affiliation: Department of Pathology and Laboratory Medicine, Western University, Ontario, Canada.|Department of Biochemistry and Pediatrics, Western University, Ontario, Canada.
authors.forename: Cathy
authors.initials: C
authors.lastname: Regan
authors.affiliation: Department of Pathology and Laboratory Medicine, Western University, Ontario, Canada.|Department of Biochemistry and Pediatrics, Western University, Ontario, Canada.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Haemato-Oncology, King's College London, London, UK.
authors.forename: Antonio
authors.initials: A
authors.lastname: Galleu
authors.affiliation: Department of Haemato-Oncology, King's College London, London, UK.
authors.forename: Francesco
authors.initials: F
authors.lastname: Dazzi
authors.affiliation: Institute of Virology, Technische Universität Dresden, Dresden, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Lindemann
authors.affiliation: Department of Pathology and Laboratory Medicine, Western University, Ontario, Canada.|Department of Biochemistry and Pediatrics, Western University, Ontario, Canada.
authors.forename: Charles Anthony
authors.initials: CA
authors.lastname: Rupar
authors.affiliation: Jefferiss Research Trust laboratories, Department of Medicine, Imperial College London, London, UK.
authors.forename: Myra Olga
authors.initials: MO
authors.lastname: McClure
mesh_terms: D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D023061:Gene Order; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D011401:Promoter Regions, Genetic; D016092:Spumavirus / Q000235:genetics*; D014161:Transduction, Genetic; D019076:Transgenes