title: SiO2 nanoparticles modified CPE as a biosensor for determination of i-motif DNA/Tamoxifen interaction.
abstract: Cytosine-rich DNA sequences can form a highly ordered structure known as i-motif in slightly acidic solutions. The stability of the folded i-motif structure is a good strategy to inhibit the telomerase reaction in cancer cells. The electrochemical biosensor was prepared by modifying carbon paste electrode with SiO2 nanoparticles to investigate drugs which can stabilize this structure. Tamoxifen (Tam), an antiestrogen hormonal agent for treatment of breast cancer, was chosen as the model ligand and its interaction with i-motif structure was examined. The interaction between i-motif DNA and Tam was studied in PBS buffer and [Fe(CN)6](3-) through the cyclic voltammetry and square wave voltammetry methods. The oxidation peak of Tam, due to the i-motif DNA/Tam interaction, was observed after i-motif immobilized on the surface of the electrode. The i-motif formation was investigated by circular dichroism spectroscopy and the results showed that this structure can certainly be made with pH around 4.5, but its stability reduced by going to the more alkaline pH. The selectivity which was studied in the presence of complementary strand demonstrated that i-motif structure could be stabilized in acidic pH even in the presence of its complementary strand.
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Heydari
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran. Electronic address: j.raoof@umz.ac.ir.
authors.forename: Jahan Bakhsh
authors.initials: JB
authors.lastname: Raoof
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ojani
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Bagheryan
mesh_terms: D015374:Biosensing Techniques; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry; D059372:Nucleotide Motifs / Q000235:genetics*; D012822:Silicon Dioxide / Q000737:chemistry; D013629:Tamoxifen / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use
chemical_list: D013629:Tamoxifen; D012822:Silicon Dioxide; D003596:Cytosine; D004247:DNA
keywords: Anti-cancer; I-motif DNA; Tamoxifen