title: [Preparation of monoclonal antibodies against His-tag and epitope analysis of cross antigens].
abstract: OBJECTIVE
  To explore the influence of His-tag on recombinant proteins in vaccination, immunization and pathogenesis.
  
  
  METHODS
  Multiple mouse monoclonal antibodies (mAb) against His-tag were prepared. The biological and immunoreactive characteristics of these mAbs and their cross-reactivity with the normal human tissues were investigated by ELISA, Western blotting and immunohistochemistry (IHC), respectively.
  
  
  RESULTS
  The binding activity of these anti-His mAbs was associated with the steric configuration of the his-tagged antigen. In addition, most of these mAbs reacted with human hemoglobin and some normal human tissues.
  
  
  CONCLUSIONS
  Anti-His antibodies could be elicited by His-tagged recombinant proteins in vivo experiments. Moreover, the functional studies of the His-tagged recombinant proteins might be affected by the reactions of anti-His6 antibodies with human hemoglobin and normal human tissues.
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China.
authors.forename: Haixiang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Wang
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China.
authors.forename: Zongli
authors.initials: Z
authors.lastname: Qi
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China. *Corresponding authors, E-mail: 1975312468@126.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Center Laboratory, Shaanxi Provincial People' s Hospital, Xi' an 710068, China. *Corresponding authors, E-mail: hjj6562@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006639:Histidine / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006639:Histidine