title: Immortalization and characterization of a new canine mammary tumour cell line FR37-CMT.
abstract: Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37-CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD-SCID mice. FR37-CMT cell line has a stellate or fusiform shape, displays the ability to reorganize the collagen matrix, expresses vimentin, CD44 and shows the loss of E-cadherin which is considered a fundamental event in epithelial to mesenchymal transition (EMT). The up-regulation of ZEB1, the detection of phosphorylated ERK1/2 and the downregulation of DICER1 and miR-200c are also in accordance with the mesenchymal characteristics of FR37-CMT cell line. FR37-CMT shows a higher resistance to cisplatin (IC50>50 µM) and to doxorubicin (IC50>5.3 µM) compared with other CMT cell lines. These results support the use of FR37-CMT as a new CMT model that may assist the understanding of the molecular mechanisms underlying EMT, CMT drug resistance, fostering the development of novel therapies targeting CMT.
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.|Centro de Química Estrutural, Complexo I, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: L R
authors.identifier: http://orcid.org/0000-0002-8637-346X
authors.initials: LR
authors.lastname: Raposo
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Roma-Rodrigues
authors.affiliation: Centro de Investigação em Biociências e Tecnologias da Saúde (CBiOS), Faculdade Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias (ULHT) Lisbon, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Faísca
authors.affiliation: Centro de Investigação em Biociências e Tecnologias da Saúde (CBiOS), Faculdade Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias (ULHT) Lisbon, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Alves
authors.affiliation: Oncovet Veterinary Clinic, Lisbon, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Henriques
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: M C
authors.initials: MC
authors.lastname: Carvalheiro
authors.affiliation: Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: M L
authors.initials: ML
authors.lastname: Corvo
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: P V
authors.initials: PV
authors.lastname: Baptista
authors.affiliation: Centro de Química Estrutural, Complexo I, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: A J
authors.initials: AJ
authors.lastname: Pombeiro
authors.affiliation: UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.|Centro de Química Estrutural, Complexo I, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: A R
authors.initials: AR
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003094:Collagen / Q000378:metabolism; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D018960:Hyaluronan Receptors / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014746:Vimentin / Q000378:metabolism
chemical_list: D018960:Hyaluronan Receptors; D014746:Vimentin; D003094:Collagen
keywords: canine mammary tumours; cisplatin; doxorubicin; epithelial to mesenchymal transition; miRNAs