title: Application of Goldmag immune probe in timely detection of syphilis based on GIS platform.
abstract: The purpose of this study was to apply goldmag immunoprobes into establishment of nanoparticles-based colorimetric assay as well as construction of immunochromatography quantitative and qualitative system by exploring point-of-care testing of syphilis with goldmag particles carrier-based immunoprobe and analysis of spatial data of Geographic Information System (GIS) platform. Goat anti-rabbit immunoglobulin G (IgG) was coupled on the surface of modified nanoparticles, taking N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide as the connector. Then the nanoparticles were used for colorimetric detection of goat-anti-rabbit IgG in liquid phase system. Based on the analysis of spatial data in GIS platform, we found the probe constructed based on MUA-Fe304/Au nanoparticles responded more sensitive to detection objects compared with the probe designed based on PAA-Fe3O4/Au nanoparticles, and its reaction rate constant was two times that of PAA-Fe3O4/Au nanoparticles based goldmag immunoprobe. Goldmag particles not only can be coupled with biomolecules such as antibody/antigen and glycoprotein but also possess superparamagnetism.
authors.affiliation: a Department of Gynecologic Oncology , Guangxi Medical University , Nanning , Guangxi , China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Tang
authors.affiliation: a Department of Gynecologic Oncology , Guangxi Medical University , Nanning , Guangxi , China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Liang
authors.affiliation: a Department of Gynecologic Oncology , Guangxi Medical University , Nanning , Guangxi , China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Nong
authors.affiliation: a Department of Gynecologic Oncology , Guangxi Medical University , Nanning , Guangxi , China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wu
authors.affiliation: a Department of Gynecologic Oncology , Guangxi Medical University , Nanning , Guangxi , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.affiliation: a Department of Gynecologic Oncology , Guangxi Medical University , Nanning , Guangxi , China.
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000097:blood*; D002234:Carbodiimides / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods; D052203:Ferrosoferric Oxide / Q000737:chemistry; D040362:Geographic Information Systems; D006041:Goats; D006046:Gold / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015335:Molecular Probes / Q000138:chemical synthesis*; D019095:Point-of-Care Systems; D011817:Rabbits; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D014210:Treponema pallidum / Q000737:chemistry* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002234:Carbodiimides; D007074:Immunoglobulin G; D058185:Magnetite Nanoparticles; D015335:Molecular Probes; D006046:Gold; D052203:Ferrosoferric Oxide
keywords: Geographic information system; goldmag immunoprobes; goldmag particles; point-of-care testing