title: Anoxybacillusgeothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station.
abstract: A novel endospore-forming bacterium designated strain GSsed3T was isolated from deposits clogging aboveground filters from the geothermal power platform of Groß Schönebeck in northern Germany. The novel isolate was Gram-staining-positive, facultatively anaerobic, catalase-positive and oxidase-positive. Optimum growth occurred at 60 °C, 0.5 % (w/v) NaCl and pH 7-8. Analysis of the 16S rRNA gene sequence similarity indicated that strain GSsed3T belonged to the genus Anoxybacillus, and showed 99.8 % sequence similarity to Anoxybacillus rupiensis R270T, 98.2 % similarity to Anoxybacillus tepidamans GS5-97T, 97.9 % similarity to Anoxybacillus voinovskiensis TH13T, 97.7 % similarity to Anoxybacillus caldiproteolyticus DSM 15730T and 97.6 % similarity to Anoxybacillus amylolyticus MR3CT. DNA-DNA hybridization (DDH) indicated only 16 % relatedness to Anoxybacillus rupiensis DSM 17127T. Furthermore, DDH estimation based on genomes analysis indicated only 19.9 % overall nucleotide similarity to Anoxybacillus amylolyticus DSM 15939T. The major respiratory menaquinone was MK-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unknown phosphoglycolipid and one unknown phospholipid. The predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0, C16 : 0, iso-C16 : 0 and anteiso-C17 : 0. The peptidoglycan type was A1γ meso-Dpm-direct. The genomic DNA G+C content of the strain was 46.9 mol%. The phenotypic, genotypic and chemotaxonomic characterization indicated that strain GSsed3T differs from related species of the genus. Therefore, strain GSsed3T is considered to be a representative of a novel species of the genus Anoxybacillus, for which the name Anoxybacillus geothermalis sp. nov. is proposed. The type strain of Anoxybacillus geothermalis is GSsed3T (=CCOS808T =ATCC BAA2555T).
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Sevasti
authors.initials: S
authors.lastname: Filippidou
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Marion
authors.initials: M
authors.lastname: Jaussi
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Junier
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Tina
authors.initials: T
authors.lastname: Wunderlin
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Nicole
authors.initials: N
authors.lastname: Jeanneret
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Fabio
authors.initials: F
authors.lastname: Palmieri
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Palmieri
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Roussel-Delif
authors.affiliation: Helmholtz Centre Potsdam, German Research Centre for Geosciences GFZ, Telegrafenberg, D-14473 Potsdam, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Vieth-Hillebrand
authors.affiliation: Helmholtz Centre Potsdam, German Research Centre for Geosciences GFZ, Telegrafenberg, D-14473 Potsdam, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Vetter
authors.affiliation: Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, 87545, USA.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Chain
authors.affiliation: Helmholtz Centre Potsdam, German Research Centre for Geosciences GFZ, Telegrafenberg, D-14473 Potsdam, Germany.
authors.forename: Simona
authors.initials: S
authors.lastname: Regenspurg
authors.affiliation: Laboratory of Microbiology, Institute of Biology, University of Neuchatel, Emile-Argand 11, CH-2000, Switzerland.
authors.forename: Pilar
authors.initials: P
authors.lastname: Junier
mesh_terms: D058677:Anoxybacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005858:Germany; D060587:Groundwater / Q000382:microbiology*; D008903:Minerals; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011210:Power Plants; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008903:Minerals; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8