title: Paracrine Factors from Irradiated Peripheral Blood Mononuclear Cells Improve Skin Regeneration and Angiogenesis in a Porcine Burn Model.
abstract: Burn wounds pose a serious threat to patients and often require surgical treatment. Skin grafting aims to achieve wound closure but requires a well-vascularized wound bed. The secretome of peripheral blood mononuclear cells (PBMCs) has been shown to improve wound healing and angiogenesis. We hypothesized that topical application of the PBMC secretome would improve the quality of regenerating skin, increase angiogenesis, and reduce scar formation after burn injury and skin grafting in a porcine model. Full-thickness burn injuries were created on the back of female pigs. Necrotic areas were excised and the wounds were covered with split-thickness mesh skin grafts. Wounds were treated repeatedly with either the secretome of cultured PBMCs (Sec(PBMC)), apoptotic PBMCs (Apo-Sec(PBMC)), or controls. The wounds treated with Apo-Sec(PBMC) had an increased epidermal thickness, higher number of rete ridges, and more advanced epidermal differentiation than controls. The samples treated with Apo-Sec(PBMC) had a two-fold increase in CD31+ cells, indicating more angiogenesis. These data suggest that the repeated application of Apo-Sec(PBMC) significantly improves epidermal thickness, angiogenesis, and skin quality in a porcine model of burn injury and skin grafting.
authors.affiliation: Division of Plastic and Reconstructive Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.|Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hacker
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria.
authors.forename: Rainer
authors.initials: R
authors.lastname: Mittermayr
authors.affiliation: Division of Plastic and Reconstructive Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Nickl
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.|Department of Trauma Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Haider
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Diana
authors.initials: D
authors.lastname: Lebherz-Eichinger
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Lucian
authors.initials: L
authors.lastname: Beer
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Mitterbauer
authors.affiliation: Division of Rheumatology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Harald
authors.initials: H
authors.lastname: Leiss
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Zimmermann
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schweiger
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Keibl
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Helmut
authors.initials: H
authors.lastname: Hofbauer
authors.affiliation: Red Cross Blood Transfusion Service of Upper Austria, Krankenhausstraße 7, 4017 Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Gabriel
authors.affiliation: Division of Cardiology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Mariann
authors.initials: M
authors.lastname: Pavone-Gyöngyösi
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstraße 13, 1200 Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.affiliation: Department of Dermatology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Erwin
authors.initials: E
authors.lastname: Tschachler
authors.affiliation: Department of Dermatology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Mildner
authors.affiliation: Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Waehringer Guertel 18-20, 1090 Vienna, Austria.|Division of Thoracic Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Hendrik Jan
authors.initials: HJ
authors.lastname: Ankersmit
mesh_terms: D000818:Animals; D002056:Burns / Q000628:therapy*; D004195:Disease Models, Animal; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000528:radiation effects*; D018919:Neovascularization, Physiologic; D012038:Regeneration; D012879:Skin Physiological Phenomena; D016038:Skin Transplantation; D013552:Swine; D016896:Treatment Outcome
chemical_list: D036341:Intercellular Signaling Peptides and Proteins