title: Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9.
abstract: CRISPR-derived RNA guided endonucleases (RGENs) have been widely used for both gene knockout and knock-in at the level of single or multiple genes. RGENs are now available for forward genetic screens at genome scale, but single guide RNA (sgRNA) selection at this scale is difficult.
  
  
  
  We develop an online tool, Cas-Database, a genome-wide gRNA library design tool for Cas9 nucleases from Streptococcus pyogenes (SpCas9). With an easy-to-use web interface, Cas-Database allows users to select optimal target sequences simply by changing the filtering conditions. Furthermore, it provides a powerful way to select multiple optimal target sequences from thousands of genes at once for the creation of a genome-wide library. Cas-Database also provides a web application programming interface (web API) for advanced bioinformatics users.
  
  
  
  Free access at http://www.rgenome.net/cas-database/
  
  
  
  sangsubae@hanyang.ac.kr or jskim01@snu.ac.kr
  
  
  
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Department of Chemistry, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Jeongbin
authors.initials: J
authors.lastname: Park
authors.affiliation: Center for Genome Engineering, Institute for Basic Science, Seoul 151-747, South Korea Department of Chemistry, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Hanyang University, Seoul 133-791, South Korea Institute for Materials Design, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Sangsu
authors.initials: S
authors.lastname: Bae
mesh_terms: D064113:CRISPR-Cas Systems; D030541:Databases, Genetic; D055786:Gene Knockout Techniques; D015723:Gene Library; D020407:Internet; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics*; D013297:Streptococcus pyogenes / Q000201:enzymology
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems