title: Simultaneous determination of thirteen kinds of amino acid and eight kinds of acylcarnitine in human serum by LC-MS/MS and its application to measure the serum concentration of lung cancer patients.
abstract: Easy-to-use early cancer detection methods based on metabolomics using serum samples have been developed recently. Among metabolites, amino acids and acylcarnitine are two of the most suitable candidates for diagnosing lung cancer. The purpose of the present study was to develop a novel, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to simultaneously determine 13 amino acids and 8 acylcarnitines in lung cancer patients in serum. After derivatization, the 21 analytes were separated using a C18column with gradient elution program in 14 min, obtaining recovery within 90.4-113.8% and precision within 0.3-14.8%. The method was successfully applied in concentration determination of lung cancer patients and healthy controls. The results showed that the serum concentration of lung cancer patients were significant from those of healthy controls.
authors.affiliation: Beijing Harmony Health Medical Diagnostics Co. Ltd, Beijing, China.|Department of Medicinal Chemistry in Harbin Medical University, Harbin, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Ni
authors.affiliation: Beijing Harmony Health Medical Diagnostics Co. Ltd, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Beijing Harmony Health Medical Diagnostics Co. Ltd, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Medicinal Chemistry in Harbin Medical University, Harbin, China. nijunjunmaster@163.com.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000097:blood*; D002331:Carnitine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000596:Amino Acids; C116917:acylcarnitine; D002331:Carnitine
keywords: LC-MS/MS; acylcarnitines; amino acids; lung cancer