title: Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats.
abstract: Understanding the relationship between brain function and behavior remains a major challenge in neuroscience. Photoacoustic tomography (PAT) is an emerging technique that allows for noninvasive in vivo brain imaging at micrometer-millisecond spatiotemporal resolution. In this article, a novel, miniaturized 3D wearable PAT (3D-wPAT) technique is described for brain imaging in behaving rats. 3D-wPAT has three layers of fully functional acoustic transducer arrays. Phantom imaging experiments revealed that the in-plane X-Y spatial resolutions were ~200 μm for each acoustic detection layer. The functional imaging capacity of 3D-wPAT was demonstrated by mapping the cerebral oxygen saturation via multi-wavelength irradiation in behaving hyperoxic rats. In addition, we demonstrated that 3D-wPAT could be used for monitoring sensory stimulus-evoked responses in behaving rats by measuring hemodynamic responses in the primary visual cortex during visual stimulation. Together, these results show the potential of 3D-wPAT for brain study in behaving rodents.
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Tang
authors.affiliation: Child Health Research Institute, Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Jason E
authors.initials: JE
authors.lastname: Coleman
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Dai
authors.affiliation: J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.|School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
authors.forename: Huabei
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001931:Brain Mapping; D059907:Functional Neuroimaging / Q000295:instrumentation / Q000379:methods*; D018496:Hyperoxia / Q000503:physiopathology; D008297:Male; D019047:Phantoms, Imaging; D010775:Photic Stimulation / Q000379:methods; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014054:Tomography / Q000295:instrumentation / Q000379:methods*; D014159:Transducers; D014793:Visual Cortex / Q000502:physiology*