title: Novel imaging tools for investigating the role of immune signalling in the brain.
abstract: The importance of neuro-immune interactions in both physiological and pathophysiological states cannot be overstated. As our appreciation for the neuroimmune nature of the brain and spinal cord grows, so does our need to extend the spatial and temporal resolution of our molecular analysis techniques. Current imaging technologies applied to investigate the actions of the neuroimmune system in both health and disease states have been adapted from the fields of immunology and neuroscience. While these classical techniques have provided immense insight into the function of the CNS, they are however, inherently limited. Thus, the development of innovative methods which overcome these limitations are crucial for imaging and quantifying acute and chronic neuroimmune responses. Therefore, this review aims to convey emerging novel and complementary imaging technologies in a form accessible to medical scientists engaging in neuroimmune research.
authors.affiliation: School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia; ARC Centre of Excellence for Nanoscale BioPhotonics, Australia.
authors.forename: Jonathan Henry W
authors.initials: JHW
authors.lastname: Jacobsen
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Australia; Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia.
authors.forename: Lindsay M
authors.initials: LM
authors.lastname: Parker
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Australia; Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia.
authors.forename: Arun V
authors.initials: AV
authors.lastname: Everest-Dass
authors.affiliation: ARC Centre of Excellence for Nanoscale BioPhotonics, Australia; Institute for Photonics and Advanced Sensing and School of Physical Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Erik P
authors.initials: EP
authors.lastname: Schartner
authors.affiliation: School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia; ARC Centre of Excellence for Nanoscale BioPhotonics, Australia; Institute for Photonics and Advanced Sensing and School of Physical Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsiminis
authors.affiliation: School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia; ARC Centre of Excellence for Nanoscale BioPhotonics, Australia.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Staikopoulos
authors.affiliation: School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia; ARC Centre of Excellence for Nanoscale BioPhotonics, Australia.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Hutchinson
authors.affiliation: School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia; ARC Centre of Excellence for Nanoscale BioPhotonics, Australia.
authors.forename: Sanam
authors.initials: S
authors.lastname: Mustafa
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000276:immunology*; D004660:Encephalitis / Q000000981:diagnostic imaging* / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D053758:Nanoparticles / Q000008:administration & dosage; D059906:Neuroimaging / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: Imaging tools; Immune signalling; Immunohistochemistry; MALDI imaging; Nanoparticles; Neuroinflammation; RNA-based probes; Raman spectroscopy; Tissue clearing