title: [Increased apoptosis and down-regulation of RhoA in HepG2 cells infected by Listeria monocytogenes].
abstract: OBJECTIVE
  To explore the apoptosis of HepG2 cells infected by Listeria monocytogenes EGD strain (Lm-EGD) as well as Rho family small GTPases RhoA expression.
  
  
  METHODS
  HepG2 cells were infected with Lm-EGD (MOI=10 and MOI=100) and collected 1 hour and 20 hours after infection. After harvesting, the apoptosis of HepG2 cells was determined by flow cytometry combined with annexin V-FITC/PI assay. RhoA and caspase 3 mRNAs were analyzed by reverse-transcription PCR. The caspase 3 activity was detected by colorimetric assay. And Western blotting was used to detect RhoA expression in HepG2 cells.
  
  
  RESULTS
  Lm invasion promoted HepG2 cell apoptosis and down-regulated RhoA mRNA and protein expression. Additionally, caspase 3 expression was up-regulated following Lm infection.
  
  
  CONCLUSIONS
  Lm infection could promote host cell apoptosis and down-regulate RhoA expression.
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xinye
authors.initials: X
authors.lastname: Han
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Danhua
authors.initials: D
authors.lastname: Su
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Fu
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xugan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China. *Corresponding author, E-mail: chensxia@ujs.edu.cn.
authors.forename: Shengxia
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D015536:Down-Regulation; D056945:Hep G2 Cells; D006801:Humans; D008089:Listeria monocytogenes / Q000472:pathogenicity*; D012333:RNA, Messenger / Q000032:analysis; D020742:rhoA GTP-Binding Protein / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; C066737:RHOA protein, human; C505407:CASP3 protein, human; D053148:Caspase 3; D020742:rhoA GTP-Binding Protein