title: pH modulates arsenic toxicity in Bacillus licheniformis DAS-2.
abstract: The toxic characteristics of arsenic species, As(V) and As(III) result in ecological risks. Arsenic tolerant bacterium was isolated and identified as the Bacillus licheniformis DAS-2 through 16SrDNA sequencing. B. licheniformis DAS-2 was efficient to tolerate and remove both the As(V)[MIC 8mM] and As(III)[MIC 6mM] from the growth medium. The potential for the removal/uptake of arsenic from the 3, 5 and 7mM As(V) enriched growth media was 100%, 60% and 35% respectively and from the 1, 3 and 5mM As(III) enrichment it was 100%, 99% and 58% respectively at neutral pH. 80% of uptake As(V) was reduced to As(III) in 3mM As(V) enrichment which was gradually decreased to only 17% at 7mM As(V) enrichment at neutral pH. The arsenic toxicity in B. licheniformis DAS-2 was found modulated by pH and was examined through alteration in growth, uptake/removal, reduction and measurement of chemical toxicity.
authors.affiliation: Laboratory of Environment and Biotechnology, Department of Botany, Patna Science College, Patna University, Patna 800005, India.
authors.forename: K
authors.initials: K
authors.lastname: Tripti
authors.affiliation: Laboratory of Environment and Biotechnology, Department of Botany, Patna Science College, Patna University, Patna 800005, India. Electronic address: shardendu77@rediffmail.com.
authors.lastname: Shardendu
mesh_terms: D001149:Arsenates / Q000378:metabolism* / Q000633:toxicity*; D018053:Arsenites / Q000378:metabolism* / Q000633:toxicity*; D000069976:Bacillus licheniformis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration
chemical_list: D001149:Arsenates; D018053:Arsenites; C015001:arsenite; C025657:arsenic acid
keywords: Arsenate [As(V)]; Arsenite [As(III)]; Bacillus licheniformis DAS-2; pH