title: MTUS1 tumor suppressor and its miRNA regulators in fibroadenoma and breast cancer.
abstract: Breast cancer is major public health problem predominantly effects female population. Current therapeutic approaches to deal with breast cancer are still lack of effectiveness. Thus, identifying/developing novel strategies to fight against breast cancer is very important. The frequent deletions at 8p21.3-22 chromosomal location nearby D8S254 marker enabled the discovery of a novel tumor suppressor gene, MTUS1. Subsequently, MTUS1 was demonstrated to be less expressed in a variety cancer types including breast cancer. Also, it is obvious that gene expression is widely regulated by miRNAs. Here, we aimed to report differential expression of MTUS1 and its regulatory miRNAs in breast cancer and fibroadenoma tissues. Dynamic analysis of MTUS1 expression levels and its miRNAs regulators were attained by Fluidigm 96×96 Dynamic Array Expression chips and reactions were performed in Fluidigm BioMark™ HD System qPCR. Consequently, MTUS1 mRNA levels were significantly diminished in breast cancer tissues and elevated in fibroadenoma tissues. Also, among MTUS1 targeting miRNAs, miR-183-5p was identified to be overexpressed in breast cancer and down-regulated in fibroadenoma tissues. Also, expression levels of MTUS1 and miR-183-5p were well correlated with clinical parameters. In particular, MTUS1 expression was found to be diminished and miR-183-5p expression was elevated with the advancing stage. In conclusion, as a potential therapeutic target, miR-183-5p can be a chief regulator of MTUS1 and MTUS1-miR-183-5p axis may have significant influence in the pathology of breast cancer.
authors.affiliation: Mugla Sitki Kocman University, Faculty of Medicine, Department of Medical Genetics, Mugla, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kara
authors.affiliation: Bahcesehir University, School of Medicine, Department of General Surgery, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kaplan
authors.affiliation: Adiyaman University, Faculty of Medicine, Department of Medical Biology, Adiyaman, Turkey. Electronic address: i.bozgeyik@gmail.com.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Bozgeyik
authors.affiliation: Mugla Sitki Kocman University, Faculty of Medicine, Department of General Surgery, Mugla, Turkey.
authors.forename: Onder
authors.initials: O
authors.lastname: Ozcan
authors.affiliation: Mugla Sitki Kocman University, Faculty of Medicine, Department of Pathology, Mugla, Turkey.
authors.forename: Ozgur Ilhan
authors.initials: OI
authors.lastname: Celik
authors.affiliation: University of Gaziantep, Faculty of Medicine, Department of Medical Biology and Genetics, Gaziantep, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Bozgeyik
authors.affiliation: Adiyaman University, Faculty of Medicine, Department of Medical Biology, Adiyaman, Turkey.
authors.forename: Onder
authors.initials: O
authors.lastname: Yumrutas
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D018226:Fibroadenoma / Q000235:genetics* / Q000473:pathology*; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D059467:Transcriptome; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C533449:MIRN183 microRNA, human; C474762:MTUS1 protein, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins
keywords: Breast cancer; Fibroadenoma; MTUS1; let-7; miR-183-5p; miRNA