title: High-efficiency extraction of nucleosides based on the combination of self-assembly ionic liquid layer and boronic acid-functionalized attapulgite.
abstract: Boronate affinity materials have been widely used for selective capture of cis-diol-containing molecules, but most do not have sufficient extraction efficiency. We have prepared boronic acid-functionalized attapulgite, and then it was coated with imidazolium-based ionic liquid, 1-dodecyl-3-methylimidazolium bromide (C12mimBr), via physical self-assembly process. The extraction efficiency of the material increased dramatically after coated with C12mimBr, and its enrichment ratios for nucleosides increased by 9- to 282-fold. Besides, the C12mimBr-coated adsorbent did not lose selectivity and was able to capture cis-diols in the presence of a 100-fold excess of interferences. C12mimBr-coated material was applied to selective enrichment of nucleosides from human urine. The limits of detection and the limits of quantification were in the range of 0.06-0.46ngmL(-1) and 0.20-1.53ngmL(-1), respectively. Reproducibility of the method was determined with relative standard deviations≤9.9%. The recoveries of the target nucleosides from spiked human urine were in the range of 87.8-109.6%. In our preception, self-assembly ionic liquid layer can serve as a promising alternative to improve the extraction efficiency of boronate affinity materials.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Shuxia
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address: zhanghx@lzu.edu.cn.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001897:Boronic Acids; D006801:Humans; D052578:Ionic Liquids; D017616:Magnesium Compounds; D009705:Nucleosides / Q000302:isolation & purification*; D015203:Reproducibility of Results; D017655:Silicon Compounds
chemical_list: D001897:Boronic Acids; D052578:Ionic Liquids; D017616:Magnesium Compounds; D009705:Nucleosides; D017655:Silicon Compounds; C026325:attapulgite
keywords: Boronate affinity; Extraction efficiency; Ionic liquid; Nucleoside; Self-assembly