title: Anti-inflammatory effects of alpinone 3-acetate from Alpinia japonica seeds.
abstract: We aimed to investigate the bioactive components of Alpinia japonica as anti-inflammatory compounds using searches of the Alpinia genus, and subsequently demonstrated that alpinone 3-acetate markedly inhibits 12-O-tetradecanoyiphorbol 13-acetate-induced inflammation in a mouse model of ear edema. To assess other bioactivities of alpinone 3-acetate, we performed translatome analyses and compared them with those of hydrocortisone. Polysome-associated mRNAs were prepared from alpinone 3-acetate- or hydrocortisone-treated and control cells from 12-O-tetradecanoyiphorbol 13-acetate-induced THP-1-derived macrophages cultured in the presence of Escherichia coli O-111 lipopolysaccharide. Subsequent microarray analysis revealed that alpinone 3-acetate and hydrocortisone upregulated and downregulated the same 155 and 41 genes, respectively. Moreover, direct comparisons of translationally regulated genes indicated 5 and 10 gene probes that were upregulated and downregulated by alpinone 3-acetate and hydrocortisone, respectively. In conclusion, assays of 12-O-tetradecanoyiphorbol 13-acetate-induced inflammation ear edema in mice and polysome profiling of alpinone 3-acetate bioactivities indicated similar medicinal possibilities to those of hydrocortisone.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo, Togane, Chiba, 283-8555, Japan. tomohito@jiu.ac.jp.
authors.forename: Tomohito
authors.initials: T
authors.lastname: Kakegawa
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo, Togane, Chiba, 283-8555, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Miyazaki
authors.affiliation: School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba, 274-8555, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Yasukawa
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005419:Flavonoids / Q000737:chemistry*; D007249:Inflammation / Q000139:chemically induced*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D012639:Seeds / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; C438066:alpinone
keywords: Alpinia japonica; Alpinone 3-acetate; Anti-inflammation; Hydrocortisone; Polysome; Translatome