title: Metabolite Profiling of Sugarcane Genotypes and Identification of Flavonoid Glycosides and Phenolic Acids.
abstract: Sugarcane is an important agricultural crop in the economy of tropical regions, and Brazil has the largest cultivated acreage in the world. Sugarcane accumulates high levels of sucrose in its stalks. Other compounds produced by sugarcane are currently not of economic importance. To explore potential coproducts, we have studied the chemical diversity of sugarcane genotypes, via metabolite profiling of leaves by NMR and LC-DAD-MS. Metabolites were identified via in-house and public databases. From the analysis of 60 HPLC-fractionated extracts, LC-DAD-MS detected 144 metabolites, of which 56 were identified (MS-MS and (1)H NMR), including 19 phenolics and 25 flavones, with a predominance of isomeric flavone C-glycosides. Multivariate analysis of the profiles from genotypes utilized in Brazilian breeding programs revealed clustering according to sugar, phenolic acid, and flavone contents.
authors.affiliation: Instituto de Quı́mica, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP) , Rua Prof. Francisco Degni 55, CEP 14800-060 Araraquara, São Paulo, Brazil.
authors.forename: Isabel D
authors.initials: ID
authors.lastname: Coutinho
authors.affiliation: Plant Biology and Crop Science Department, Rothamsted Research , Harpenden AL5 2JQ, United Kingdom.
authors.forename: John M
authors.initials: JM
authors.lastname: Baker
authors.affiliation: Plant Biology and Crop Science Department, Rothamsted Research , Harpenden AL5 2JQ, United Kingdom.
authors.forename: Jane L
authors.initials: JL
authors.lastname: Ward
authors.affiliation: Plant Biology and Crop Science Department, Rothamsted Research , Harpenden AL5 2JQ, United Kingdom.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Beale
authors.affiliation: Instituto Agronômico - Centro de Cana , Rodovia Antonio Duarte Nogueira Km 321, CP 206, CEP 14032-800 Ribeirão Preto, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Creste
authors.affiliation: Instituto de Quı́mica, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP) , Rua Prof. Francisco Degni 55, CEP 14800-060 Araraquara, São Paulo, Brazil.
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Cavalheiro
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005838:Genotype; D006027:Glycosides / Q000737:chemistry* / Q000378:metabolism; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000378:metabolism; D055432:Metabolomics; D031786:Saccharum / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D006027:Glycosides; D062385:Hydroxybenzoates; C017616:phenolic acid
keywords: amino acids; flavones; metabolite profiling; phenolic acid; sugarcane