title: Introducing dengue vaccine: Implications for diagnosis in dengue vaccinated subjects.
abstract: Diagnosis of dengue virus infections is complicated by preference for different diagnostic tests in different post onset days of illness and the presence of multiple serotypes leading to secondary and tertiary infections. The sensitivity of the most commonly employed diagnostic assays such as anti dengue IgM capture (MAC) ELISA and non structural protein (NS) 1 capture ELISA are lower in secondary and subsequent infections. Introduction of dengue vaccine in endemic regions will affect the way how dengue is diagnosed in vaccinated subjects. This viewpoint article discusses implications of introduction of dengue vaccine on the diagnosis of dengue infections in vaccinated subjects and the strategies that are needed to tackle the issue.
authors.affiliation: Dengue/Chikungunya Group, National Institute of Virology (Indian Council of Medical Research), 20A Dr Ambedkar Road, Pune 411001, Maharashtra, India. Electronic address: alagarasu@gmail.com.
authors.forename: Kalichamy
authors.initials: K
authors.lastname: Alagarasu
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D003715:Dengue / Q000175:diagnosis* / Q000517:prevention & control; D053059:Dengue Vaccines / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D012698:Serologic Tests; D017361:Viral Nonstructural Proteins / Q000097:blood
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D053059:Dengue Vaccines; D007075:Immunoglobulin M; D017361:Viral Nonstructural Proteins
keywords: Dengue; Dengvaxia; Diagnosis; IgM; NS1 antigen ELISA