title: Effects of naringin on learning and memory dysfunction induced by gp120 in rats.
abstract: The aim of the present study was to investigate the effects of naringin on learning and memory dysfunction induced by HIV-1-enveloped protein gp120 in rats, and to identify its potential mechanisms of action. Learning and memory ability was evaluated via Morris water maze test, P2X7 receptor and P65 protein expressions in the rat hippocampus were detected by western blot analysis, and P2X7 mRNA expression in the hippocampus was measured by RT-PCR. We also recorded P2X7 agonist BzATP-activated current in the hippocampus via patch clamp technique. The results showed that naringin treatment (30mg/kg/day) markedly decreased the escape latency and target platform errors of rats treated with gp120 (50ng/day), and further, that naringin treatment significantly decreased the expression of P2X7 and P65 protein and P2X7 mRNA in the hippocampus of gp120-treated rats. In addition, naringin treatment reduced BzATP-activated current in the hippocampus of gp120-treated rats. These results altogether demonstrated that naringin can improve gp120-induced learning and memory dysfunction via mechanisms involving the inhibition of P2X7 expression in the hippocampus.
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Qin
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Second Clinical Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Second Clinical Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China.
authors.forename: Dalei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang 330006, PR China. Electronic address: xuchangshui@ncu.edu.cn.
authors.forename: Changshui
authors.initials: C
authors.lastname: Xu
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004195:Disease Models, Animal; D044950:Flavanones / Q000494:pharmacology / Q000627:therapeutic use*; D005786:Gene Expression Regulation / Q000187:drug effects; D015699:HIV Envelope Protein gp120; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007859:Learning Disabilities / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced* / Q000188:drug therapy*; D017628:Microglia / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism
chemical_list: D044950:Flavanones; D015699:HIV Envelope Protein gp120; D010975:Platelet Aggregation Inhibitors; D058920:Purinergic P2X Receptor Antagonists; D058486:Receptors, Purinergic P2X7; C033901:3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate; C005274:naringin
keywords: Gp120; HIV-1 associated dementia; Naringin; P2X(7) receptor