title: Comprehensive quantitative analysis of Chinese patent drug YinHuang drop pill by ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry.
abstract: YinHuang drop pill (YHDP) is a new preparation, derived from the traditional YinHuang (YH) decoction. Since drop pills are one of the newly developed forms of Chinese patent drugs, not much research has been done regarding the quality and efficacy. This study aims to establish a comprehensive quantitative analysis of the chemical profile of YHDP. ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to identify 34 non-sugar small molecules including 15 flavonoids, 9 phenolic acids, 5 saponins, 1 iridoid, and 4 iridoid glycosides in YHDP samples, and 26 of them were quantitatively determined. Sugar composition of YHDP in terms of fructose, glucose and sucrose was examined via a high performance liquid chromatography-evaporative light scattering detector on an amide column (HPLC-NH2P-ELSD). Macromolecules were examined by high performance gel permeation chromatography coupled with ELSD (HPGPC-ELSD). The content of the drop pill's skeleton component PEG-4000 was also quantified via ultra-high performance liquid chromatography coupled with charged aerosol detector (UHPLC-CAD). The results showed that up to 73% (w/w) of YHDP could be quantitatively determined. Small molecules accounted for approximately 5%, PEG-4000 represented 68%, while no sugars or macromolecules were found. Furthermore, YHDP showed no significant differences in terms of daily dosage, compared to YinHuang granules and YinHuang oral liquid; however, it has a higher small molecules content compared to YinHuang lozenge.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Tin-Long
authors.initials: TL
authors.lastname: Wong
authors.affiliation: State Key Lab of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences,Hong Kong, China.
authors.forename: Ya-Qi
authors.initials: YQ
authors.lastname: An
authors.affiliation: State Key Lab of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences,Hong Kong, China.
authors.forename: Bing-Chao
authors.initials: BC
authors.lastname: Yan
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Rui-Qi
authors.initials: RQ
authors.lastname: Yue
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Tian-Bo
authors.initials: TB
authors.lastname: Zhang
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Hing-Man
authors.initials: HM
authors.lastname: Ho
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Tian-Jing
authors.initials: TJ
authors.lastname: Ren
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Hau-Yee
authors.initials: HY
authors.lastname: Fung
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Chung-Hang
authors.initials: CH
authors.lastname: Leung
authors.affiliation: Zhejiang WeiKang Pharmaceutical Co., Ltd, China.
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Liu
authors.affiliation: State Key Lab of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences,Hong Kong, China. Electronic address: pujianxin@mail.kib.ac.cn.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Pu
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. Electronic address: simonhan@hkbu.edu.hk.
authors.forename: Quan-Bin
authors.initials: QB
authors.lastname: Han
authors.affiliation: State Key Lab of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences,Hong Kong, China.
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Sun
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Chinese medicine formulas; Quality evaluation; Quantitative analysis; YinHuang drop pill