title: Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae).
abstract: OBJECTIVE
  The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami's urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae).
  
  
  RESULTS
  Ten microsatellites were developed for each species. For E. tampensis sampled from the natural population, allele numbers ranged from one to four, with an average observed heterozygosity (H o) of 0.314 and average expected heterozygosity (H e) of 0.281. For the individuals from cultivation, allele numbers ranged from one to six, with an average H o of 0.35 and an average H e of 0.224. For C. punctatum, allele numbers ranged from one to three, with an average H o of 0.257 and an average H e of 0.272.
  
  
  CONCLUSIONS
  These microsatellites will be used to assess the genetic diversity of natural and cultivated populations with the intention of guiding genetic breeding under the Million Orchid Project.
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA; Department of Biology, University of Miami, 1301 Memorial Drive, Coral Gables, Florida 33126 USA.
authors.forename: Joanna
authors.initials: J
authors.lastname: Weremijewicz
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Jasmin I
authors.initials: JI
authors.lastname: Almonte
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Vanessa S
authors.initials: VS
authors.lastname: Hilaire
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Frank D
authors.initials: FD
authors.lastname: Lopez
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Lu
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Marrero
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Martinez
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Edson A
authors.initials: EA
authors.lastname: Zarate
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Ana K
authors.initials: AK
authors.lastname: Lam
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Samantha A N
authors.initials: SA
authors.lastname: Ferguson
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Nicolas Z
authors.initials: NZ
authors.lastname: Petrakis
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Kelsey A
authors.initials: KA
authors.lastname: Peeples
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Ebony D
authors.initials: ED
authors.lastname: Taylor
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Natalie M
authors.initials: NM
authors.lastname: Leon
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Carolina
authors.initials: C
authors.lastname: Valdes
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Hass
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Reeve
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA.
authors.forename: Danielle T
authors.initials: DT
authors.lastname: Palow
authors.affiliation: Fairchild Tropical Botanic Garden, 10901 Old Cutler Road, Coral Gables, Florida 33156 USA; Department of Biological Sciences, Florida International University, 11200 SW 8th Street, Miami, Florida 33199 USA.
authors.forename: Jason L
authors.initials: JL
authors.lastname: Downing
keywords: Cyrtopodium punctatum; Encyclia tampensis; Orchidaceae; commercially exploited plants; microsatellites; native Florida orchids