title: Proteomic Insight into the Response of Arabidopsis Chloroplasts to Darkness.
abstract: Chloroplast function in photosynthesis is essential for plant growth and development. It is well-known that chloroplasts respond to various light conditions. However, it remains poorly understood about how chloroplasts respond to darkness. In this study, we found 81 darkness-responsive proteins in Arabidopsis chloroplasts under 8 h darkness treatment. Most of the proteins are nucleus-encoded, indicating that chloroplast darkness response is closely regulated by the nucleus. Among them, 17 ribosome proteins were obviously reduced after darkness treatment. The protein expressional patterns and physiological changes revealed the mechanisms in chloroplasts in response to darkness, e.g., (1) inhibition of photosystem II resulted in preferential cyclic electron flow around PSI; (2) promotion of starch degradation; (3) inhibition of chloroplastic translation; and (4) regulation by redox and jasmonate signaling. The results have improved our understanding of molecular regulatory mechanisms in chloroplasts under darkness.
authors.affiliation: Department of Mathematics, College of Mathematics and Science, Shanghai Normal University, Shanghai, P.R. China.|Institute of Plant Gene Function, Shanghai Normal University, Shanghai, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Plant Gene Function, Shanghai Normal University, Shanghai, P.R. China.|Department of Biology, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, P.R. China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Institute of Plant Gene Function, Shanghai Normal University, Shanghai, P.R. China.|Department of Biology, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, P.R. China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Mathematics, College of Mathematics and Science, Shanghai Normal University, Shanghai, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, United States of America.
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Plant Gene Function, Shanghai Normal University, Shanghai, P.R. China.|Department of Biology, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, P.R. China.
authors.forename: Zhongnan
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Biology, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, P.R. China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dai
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000528:radiation effects; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism* / Q000528:radiation effects*; D003624:Darkness; D010788:Photosynthesis / Q000528:radiation effects; D040901:Proteomics; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013213:Starch / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002734:Chlorophyll; D013213:Starch; D012273:Ribulose-Bisphosphate Carboxylase