title: Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
abstract: In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation. However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions. The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum. The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
authors.affiliation: Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, 28 Jagiellońska St., 40-032, Katowice, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Gawecki
authors.affiliation: Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, 28 Jagiellońska St., 40-032, Katowice, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sala
authors.affiliation: Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, 28 Jagiellońska St., 40-032, Katowice, Poland.
authors.forename: Ewa U
authors.initials: EU
authors.lastname: Kurczyńska
authors.affiliation: Department of Animal Histology and Embryology, University of Silesia, 9 Bankowa St., 40-007, Katowice, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Świątek
authors.affiliation: Department of Plant Cytology and Embryology, Jagiellonian University in Kraków, 9 Gronostajowa St., 30-387, Kraków, Poland. bartosz.plachno@uj.edu.pl.
authors.forename: Bartosz J
authors.initials: BJ
authors.lastname: Płachno
mesh_terms: D060832:Apomixis; D056625:Endosperm / Q000166:cytology; D000939:Epitopes / Q000378:metabolism*; D007150:Immunohistochemistry; D009088:Mucoproteins / Q000378:metabolism*; D056771:Ovule / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D010368:Pectins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D031205:Taraxacum / Q000196:embryology / Q000378:metabolism* / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000939:Epitopes; D009088:Mucoproteins; D010940:Plant Proteins; D011134:Polysaccharides; C006619:arabinogalactan proteins; C007916:hemicellulose; D010368:Pectins
keywords: Apomixis; Arabinogalactan proteins; Asteraceae; Dandelions; Gynoecium; Hemicellulose; Ovule; Pectins; Taraxacum; Transmitting tissue