title: Synergistic activity between an antimicrobial polyacrylamide and daptomycin versus Staphylococcus aureus biofilm.
abstract: Antibiotic resistance of bacteria growing in biofilms compared to their planktonic counterparts enhances the difficulty to eradicate biofilm-associated infections. In the last decade, combination antibiotic therapy has emerged as an attractive strategy for treating biofilm infections, even if in most of tolerant biofilms the optimal combinations are still unknown. In this study, an antimicrobial cationic polyacrylamide was used in combination with daptomycin or moxifloxacin against mature biofilms of Staphylococcus aureus clinical isolates to examine a possible improvement of the antibiofilm activity of the two antibiotics. The polymer did not have an effect on moxifloxacin but significantly increased the antibiofilm efficacy of daptomycin. These findings are presumably related to the different mechanism of action of the two drugs. In summary, our data highlighted the ability of polycations to increase daptomycin antibiofilm activity providing a potential strategy to eradicate biofilms in industrial or medical settings.
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: Wafi
authors.initials: W
authors.lastname: Siala
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: Françoise
authors.initials: F
authors.lastname: Van Bambeke
authors.affiliation: School of Pharmacy, University of Nottingham, NG7 2RD, UK.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Taresco
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Piozzi
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy iolanda.francolini@uniroma1.it.
authors.forename: Iolanda
authors.initials: I
authors.lastname: Francolini
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D017576:Daptomycin / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D004357:Drug Synergism; D006801:Humans; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000890:Anti-Infective Agents; D017576:Daptomycin; D000099166:Polyacrylamides
keywords: antibiotic resistance; daptomycin; drug/polymer combinations; microbial biofilms; moxifloxacin; polycations