title: Polymorphisms in the CHIT1 gene: Associations with colorectal cancer.
abstract: Colorectal cancer (CRC) is one of the most common solid tumors worldwide, often associated with inflammation. The microbes in the human intestine have a key role in inflammations and CRC. Chitotriose renders growth advantage to some bacteria, especially some pathogens, and thus has a role in inflammations. The enzyme chitotriosidase, encoded by the CHIT1 gene of the host, may degrade chitotriose with different efficiencies depending on the alleles. We sequenced the CHIT1 gene for 320 Chinese Han CRC patients and 404 normal controls, and focused on variations rs61745299 and rs35920428 within the CHIT1 gene for their possible roles in CRC. Statistical analyses were conducted using Chi-Square Tests as implemented in SPSS (version 19.0). Multiple sequence alignment was conducted using the Vector NTI, and protein expression levels were analyzed by western blotting. The two variations, rs61745299 and rs35920428 within the CDS region of CHIT1 gene, were associated with the risk of CRC (both with P values < 0.001). Western blotting analysis showed that the variations increased the expression levels of the CHIT1 and C-reaction protein genes in the cancer tissue. We conclude that the two variations of CHIT1, rs61745299 and rs35920428, increase expression of the gene and are associated with CRC in Chinese Han populations.
authors.affiliation: Genomics Research Center, State-Province Key Laboratory of Biopharmaceutical Engineering, Harbin Medical University, Harbin, China.|Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Heilongjiang, China.
authors.forename: Fei-Feng
authors.initials: FF
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yan
authors.affiliation: Department of Colorectal Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Zhi-Xun
authors.initials: ZX
authors.lastname: Zhao
authors.affiliation: Department of Colorectal Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Colorectal Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Song
authors.affiliation: Department of Colorectal Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xing-Wang
authors.initials: XW
authors.lastname: Zhao
authors.affiliation: Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Heilongjiang, China.|Department of Colorectal Surgery, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Xi-Shan
authors.initials: XS
authors.lastname: Wang
authors.affiliation: Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Heilongjiang, China.|Department of Colorectal Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Gui-Yu
authors.initials: GY
authors.lastname: Wang
authors.affiliation: Genomics Research Center, State-Province Key Laboratory of Biopharmaceutical Engineering, Harbin Medical University, Harbin, China.|Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Heilongjiang, China.|Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Canada.
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Liu
mesh_terms: D000368:Aged; D000483:Alleles; D002681:China; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D000069196:Gastrointestinal Microbiome; D005787:Gene Frequency; D005838:Genotype; D006596:Hexosaminidases / Q000235:genetics*; D006801:Humans; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D020641:Polymorphism, Single Nucleotide; D012306:Risk
chemical_list: D006596:Hexosaminidases; C086400:chitotriosidase
keywords: C-reaction protein; CHIT1; colorectal cancer; gene expression; microbe