title: Development and characterization of EST-SSR markers for Catalpa bungei (Bignoniaceae).
abstract: OBJECTIVE
  Catalpa bungei (Bignoniaceae) is a deciduous tree native to China. We developed microsatellite markers for C. bungei to investigate its population genetics.
  
  
  RESULTS
  One hundred seventy-seven expressed sequence tag (EST)-simple sequence repeat (SSR) primer pairs were isolated and characterized using next-generation sequencing. Thirty of these primer pairs were polymorphic loci in 52 individuals of C. bungei. The number of alleles ranged from two to 18 with observed and expected heterozygosity values of 0.05-1.00 and 0.18-0.95, respectively. The fixation index ranged from -1.00 to 1.00 with an average of 0.32. No linkage disequilibrium was detected in any pair of loci. All markers showed good amplification results in four species (C. bungei, C. fargesii, C. duclouxii, and C. ovata) except three loci.
  
  
  CONCLUSIONS
  These polymorphic markers are expected to be helpful in further studies on the systematics and phylogeography of C. bungei and related species.
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Ma
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Shu'an
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Linfang
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Rutong
authors.initials: R
authors.lastname: Yang
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu Province, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
keywords: Bignoniaceae; Catalpa bungei; RNA-seq; expressed sequence tags; population genetics; simple sequence repeats (SSRs)