title: In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules.
abstract: We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol.
authors.affiliation: Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States.
authors.forename: Kyung-Ae
authors.initials: KA
authors.lastname: Yang
authors.affiliation: Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
authors.affiliation: Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States; Department of Biomedical Engineering, Columbia University, New York, NY 10032, United States. Electronic address: mns18@cumc.columbia.edu.
authors.forename: Milan N
authors.initials: MN
authors.lastname: Stojanovic
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods*; D009690:Nucleic Acid Conformation; D052156:SELEX Aptamer Technique / Q000379:methods*; D054852:Small Molecule Libraries / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D054852:Small Molecule Libraries
keywords: Aptamer; Biosensor; SELEX; Small molecules; Structure-switching