title: Nicotinamide N-methyltransferase: a potential biomarker for worse prognosis in gastric carcinoma.
abstract: In clinical practice, cancer stage (or grade) and some biomarkers, such as carcinoembryonic antigen (CEA) and CA199, are widely used to predict the prognosis of gastric carcinoma patients. Due to the limited role of prognostic indicators for gastric carcinoma, this condition remains one of the most fatal human malignancies with a dismal prognosis. Nicotinamide N-methyltransferase (NNMT, EC.2.1.1.1), a metabolizing enzyme, is involved in the development and progression of various carcinomas. However, the prognostic and biological functions of NNMT in gastric carcinoma are not yet clear. In the present study, NNMT was found to be overexpressed at the mRNA and protein levels in gastric carcinoma tissues compared with adjacent tissues. Importantly, the survival analysis verified that NNMT expression is an independent prognostic factor for overall survival of gastric cancer patients. Moreover, NNMT expression was related to primary tumor size, lymph node metastasis, distant metastasis, and TNM (tumor, node, and metastasis) stage. We also demonstrated that knockdown of NNMT inhibits cellular proliferation, invasion and migration in vitro and in vivo. Overall, the results of this study suggest that NNMT is a promising prognostic predictor for gastric cancer patients and could be used as a new target for gastric cancer therapy.
authors.affiliation: Department of Oncology, Second Affiliated Hospital, Nanjing Medical UniversityJiangsu, China; Nanjing Medical UniversityJiangsu, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Nanjing Medical University Jiangsu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Nanjing Medical University Jiangsu, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Huang
authors.affiliation: Nanjing Medical University Jiangsu, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yong
authors.affiliation: Nanjing Medical University Jiangsu, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Shen
authors.affiliation: Nanjing Medical University Jiangsu, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Nanjing Medical UniversityJiangsu, China; Huadong Medical Institute of BiotechniquesNanjing 210029, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Oncology, Second Affiliated Hospital, Nanjing Medical University Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ni
authors.affiliation: Nanjing Medical UniversityJiangsu, China; Jiangsu Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical UniversityNanjing 210029, China.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Feng
keywords: Nicotinamide N-methyltransferase; gastric carcinoma; prognosis; tissue microarray