title: Recent adaptations of fluorescence techniques for the determination of mechanistic parameters of helicases and translocases.
abstract: Helicases and translocases are nucleic acid (NA)-based molecular motors that use the free energy liberated during the nucleoside triphosphate (NTP, usually ATP) hydrolysis cycle for unidirectional translocation along their NA (DNA, RNA or heteroduplex) substrates. Determination of the kinetic and thermodynamic parameters of their mechanoenzymatic cycle serves as a basis for the exploration of their physiological behavior and various cellular functions. Here we describe how recent adaptations of fluorescence-based solution kinetic methods can be used to determine practically all important mechanistic parameters of NA-based motor proteins. We outline practically useful analysis procedures for equilibrium, steady-state and transient kinetic data. This analysis can be used to quantitatively characterize the enzymatic steps of the NTP hydrolytic cycle, the binding site size, stoichiometry and energetics of protein-NA interactions, the rate and processivity of translocation along and unwinding of NA strands, and the mechanochemical coupling between these processes. The described methods yield insights into the functional role of the enzymes, and also greatly aid the design and interpretation of single-molecule experiments as well as the engineering of enzymatic properties for biotechnological applications.
authors.affiliation: ELTE-MTA "Momentum" Motor Enzymology Research Group, Department of Biochemistry, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary. Electronic address: mate.gyimesi@ttk.elte.hu.
authors.forename: Máté
authors.initials: M
authors.lastname: Gyimesi
authors.affiliation: ELTE-MTA "Momentum" Motor Enzymology Research Group, Department of Biochemistry, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary. Electronic address: gabor.harami@gmail.com.
authors.forename: Gábor M
authors.initials: GM
authors.lastname: Harami
authors.affiliation: ELTE-MTA "Momentum" Motor Enzymology Research Group, Department of Biochemistry, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary. Electronic address: srablue@gmail.com.
authors.forename: Zsuzsa S
authors.initials: ZS
authors.lastname: Kocsis
authors.affiliation: ELTE-MTA "Momentum" Motor Enzymology Research Group, Department of Biochemistry, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary. Electronic address: mihaly.kovacs@ttk.elte.hu.
authors.forename: Mihály
authors.initials: M
authors.lastname: Kovács
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000235:genetics*; D001665:Binding Sites; D004247:DNA / Q000235:genetics; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics*; D005453:Fluorescence; D006868:Hydrolysis; D007700:Kinetics; D009696:Nucleic Acids / Q000737:chemistry / Q000235:genetics*; D012313:RNA / Q000235:genetics; D013816:Thermodynamics
chemical_list: D009696:Nucleic Acids; D012313:RNA; D000255:Adenosine Triphosphate; D004247:DNA; D004265:DNA Helicases
keywords: ATP; DNA; Helicase; Hydrolysis; Kinetics; Mechanism; NTP; Nucleic acid; RNA; Thermodynamics; Translocase; Translocation; Unwinding