title: [Inhibitory effects of pseudolaric acid B on inflammatory response and M1 phenotype polarization in RAW264.7 macrophages induced by lipopolysaccharide].
abstract: OBJECTIVE
  To investigate the effects of pseudolaric acid B (PLAB) on the inflammatory response and M1 phenotype polarization in RAW264.7 cells induced by lipopolysaccharide (LPS) and the related mechanisms.
  
  
  METHODS
  The inflammatory model in vitro was made using RAW264.7 cells stimulated by LPS, and then was treated with 0.5 μmol/L PLAB and 1 μmol/L GW9662, a peroxisome proliferators-activated receptor γ (PPARγ) antagonist. The cell cycle was tested by flow cytometry. The mRNA expressions of PPARγ and M1 phenotype markers interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) were measured by real-time PCR. The expression levels of signal molecules involved in nuclear factor-κB (NF-κB) signal pathway were detected by Western blotting.
  
  
  RESULTS
  PLAB markedly decreased the expressions of IL-1β and TNF-α mRNAs induced by LPS and increased PPARγ mRNA level. Moreover, the expressions of NF-κB p65, pNF-κB p65, IKKα, IKKβ, pIKKα/β, IκBα and pIκBα decreased in PLAB-treated cells. Meanwhile, RAW264.7 cells were arrested in G0 and G2 phase after the treatment with PLAB. However, the effects of PLAB on RAW264.7 cells could be reversed by GW9662 obviously.
  
  
  CONCLUSIONS
  PLAB could inhibit the inflammatory response and M1 phenotype polarization in RAW264.7 cells induced by LPS via modulating cell cycle and NF-κB/PPARγ signal pathway.
authors.affiliation: Postgraduate Training Base, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Liaoning Medical University, Tianjin 300162; Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162, China.
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162; Department of Pathogenic Organism and Immunology, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300309, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Ji
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162, China. *Corresponding author, E-mail: xzhou@live.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics College of the Chinese People' s Armed Police Force, Tianjin 300162, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Li
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016764:Cell Polarity / Q000187:drug effects*; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D016328:NF-kappa B / Q000502:physiology; D047495:PPAR gamma / Q000502:physiology; D010641:Phenotype; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha; C058391:pseudolaric acid B