title: Quantitative blood flow velocity imaging using laser speckle flowmetry.
abstract: Laser speckle flowmetry suffers from a debated quantification of the inverse relation between decorrelation time (τc) and blood flow velocity (V), i.e. 1/τc = αV. Using a modified microcirculation imager (integrated sidestream dark field - laser speckle contrast imaging [SDF-LSCI]), we experimentally investigate on the influence of the optical properties of scatterers on α in vitro and in vivo. We found a good agreement to theoretical predictions within certain limits for scatterer size and multiple scattering. We present a practical model-based scaling factor to correct for multiple scattering in microcirculatory vessels. Our results show that SDF-LSCI offers a quantitative measure of flow velocity in addition to vessel morphology, enabling the quantification of the clinically relevant blood flow, velocity and tissue perfusion.
authors.affiliation: Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands.|ARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney 2109, NSW Australia.
authors.forename: Annemarie
authors.initials: A
authors.lastname: Nadort
authors.affiliation: Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands.
authors.forename: Koen
authors.initials: K
authors.lastname: Kalkman
authors.affiliation: Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands.
authors.forename: Ton G
authors.initials: TG
authors.lastname: van Leeuwen
authors.affiliation: Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands.
authors.forename: Dirk J
authors.initials: DJ
authors.lastname: Faber
mesh_terms: D001783:Blood Flow Velocity; D006801:Humans; D007834:Lasers; D061848:Optical Imaging / Q000379:methods*; D012212:Rheology / Q000379:methods*