title: Osteoporosis Recovery by Antrodia camphorata Alcohol Extracts through Bone Regeneration in SAMP8 Mice.
abstract: Antrodia camphorata has previously demonstrated the efficacy in treating cancer and anti-inflammation. In this study, we are the first to evaluate Antrodia camphorata alcohol extract (ACAE) for osteoporosis recovery in vitro with preosteoblast cells (MC3T3-E1) and in vivo with an osteoporosis mouse model established in our previous studies, ovariectomized senescence accelerated mice (OVX-SAMP8). Our results demonstrated that ACAE treatment was slightly cytotoxic to preosteoblast at 25 μg/mL, by which the osteogenic gene expression (RUNX2, OPN, and OCN) was significantly upregulated with an increased ratio of OPG to RANKL, indicating maintenance of the bone matrix through inhibition of osteoclastic pathway. Additionally, evaluation by Alizarin Red S staining showed increased mineralization in ACAE-treated preosteoblasts. For in vivo study, our results indicated that ACAE inhibits bone loss and significantly increases percentage bone volume, trabecular bone number, and bone mineral density in OVX-SAMP8 mice treated with ACAE. Collectively, in vitro and in vivo results showed that ACAE could promote osteogenesis and prevent bone loss and should be considered an evidence-based complementary and alternative medicine for osteoporosis therapy through the maintenance of bone health.
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei 110, Taiwan; School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Hen-Yu
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan.
authors.forename: Chiung-Fang
authors.initials: CF
authors.lastname: Huang
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Chun-Hao
authors.initials: CH
authors.lastname: Li
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Chen
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Hong-Jian
authors.initials: HJ
authors.lastname: Wei
authors.affiliation: Department of Food and Nutrition, Providence University, Taichung 433, Taiwan.
authors.forename: Ming-Fu
authors.initials: MF
authors.lastname: Wang
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan; Department of Biotechnology, Asia University, Taichung 413, Taiwan.
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.affiliation: Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei 106, Taiwan; College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Mei-Leng
authors.initials: ML
authors.lastname: Cheong
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan; Institute of Medicine, Fu Jen Catholic University, Taipei 242, Taiwan.
authors.forename: Win-Ping
authors.initials: WP
authors.lastname: Deng