title: Furan-PNA: a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA.
abstract: We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy. After introducing furan-containing building blocks into a PNA sequence, hybridization and furan-oxidation based crosslinking to DNA is investigated. The structure of the crosslinked products is characterized and preliminary investigations concerning the application of these systems to double stranded DNA are shown.
authors.affiliation: Department of Chemistry, University of Parma, Parco Area delle Scienze 17/A, Parma 43124, Italy. alex.manicardi@unipr.it.
authors.forename: A
authors.initials: A
authors.lastname: Manicardi
authors.forename: E
authors.initials: E
authors.lastname: Gyssels
authors.forename: R
authors.initials: R
authors.lastname: Corradini
authors.forename: A
authors.initials: A
authors.lastname: Madder
mesh_terms: D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics; D005663:Furans / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D005663:Furans; D020135:Peptide Nucleic Acids