title: Suppressive Effects of Copper Sulfate Accumulation on the Spermatogenesis of Rats.
abstract: This study investigated the effect of copper sulfate (CuSO4) in the rat spermatogenesis. Forty male rats, weighing 70-80 g, were randomly divided into four groups: control group (CG, 0 mg/kg BW), low-dose group (LG, 100 mg/kg BW), mid-dose group (MG, 200 mg/kg BW), and high-dose group (HG, 400 mg/kg BW). Rats were administered CuSO4by gavage for 30 days. A variety of measurements were taken including the testis coefficients, the sperm count, the abnormal malformation rate, testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) concentrations in the serum. In addition, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA) concentration in the testis were determined. The results showed that in the CuSO4-treated groups, the testis coefficients, sperm count, T, LH, and FSH concentrations, and GSH-Pxand SOD activities decreased, while the abnormal malformation rate and MDA concentration increased, compared with the CG. It indicates that CuSO4exposure impairs the sperm quality and inhibits secretion of sex hormone and gonadotropin, and testis anti-oxidative function, suppressing the rat spermatogenesis.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.|Police Dog Technical School of Ministry of Public Security, Shenyang, 110034, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: C C
authors.initials: CC
authors.lastname: Zhuang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: F B
authors.initials: FB
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. liyanfei@neau.edu.cn.
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
mesh_terms: D000818:Animals; D019327:Copper Sulfate / Q000493:pharmacokinetics / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D012739:Gonadal Steroid Hormones / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012739:Gonadal Steroid Hormones; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D019327:Copper Sulfate
keywords: Anti-oxidative function; Copper sulfate; Rat; Sex hormone; Spermatogenesis