title: Competitive Adsorption of Plasma Proteins Using a Quartz Crystal Microbalance.
abstract: Proteins that get adsorbed onto the surfaces of biomaterials immediately upon their implantation mediate the interactions between the material and the environment. This process, in which proteins in a complex mixture compete for adsorption sites on the surface, is determined by the physicochemical interactions at the interface. Competitive adsorption of bovine serum albumin (BSA), fibronectin (Fn), and collagen type I (Col I), sequentially and from mixtures, was investigated so as to understand the performances of different surfaces used in biomedical applications. A quartz crystal microbalance with dissipation was used to monitor the adsorption of these proteins onto two materials used in functional bone replacement, a titanium alloy (Ti6Al4V) and Ti6Al4V physisorbed with poly(sodium styrenesulfonate) [poly(NaSS)], and three controls, gold, poly(desaminotyrosyltyrosine ethyl ester carbonate) [poly(DTEc)], and polystyrene (PS). In experiments with individual proteins, the adsorption was the highest with Fn and Col I and the least with BSA. Also, protein adsorption was the highest on poly(NaSS) and Ti6Al4V and the least on poly(DTEc). In sequential adsorption experiments, protein exchange was observed in BSA + Fn, Fn + Col I, and BSA + Col I sequences but not in Fn + BSA and Col I + BSA because of the lower affinity of BSA to surfaces relative to Fn and Col I. Protein adsorption was the highest with Col I + Fn on hydrophobic surfaces. In experiments with protein mixtures, with BSA & Fn, Fn appears to be preferentially adsorbed; with Fn & Col I, both proteins were adsorbed, probably as multilayers; and with Col I & BSA, the total amount of protein was the highest, greater than that in sequential and individual adsorption of the two proteins, probably because of the formation of BSA and Col I complexes. Protein conformational changes induced by the adsorbing surfaces, protein-protein interactions, and affinities of proteins appear to be the important factors that govern competitive adsorption. The findings reported here will be useful in understanding the host response to surfaces used for implants.
authors.affiliation: Laboratory of Biomaterials and Specialty Polymers, LBPS-CSPBAT CNRS UMR 7244, Institut Galilée, Université Paris 13 , 93430 Villetaneuse, France.
authors.forename: Helena P
authors.initials: HP
authors.lastname: Felgueiras
authors.affiliation: New Jersey Center for Biomaterials, Rutgers University , 145 Bevier Road, Piscataway, New Jersey 08854, United States.
authors.forename: N Sanjeeva
authors.initials: NS
authors.lastname: Murthy
authors.affiliation: New Jersey Center for Biomaterials, Rutgers University , 145 Bevier Road, Piscataway, New Jersey 08854, United States.
authors.forename: Sven D
authors.initials: SD
authors.lastname: Sommerfeld
authors.affiliation: I3S - Institute for Innovation and Health, University of Porto , Rua Alfredo Allen 208, 4200-135 Porto, Portugal.|INEB - Institute of Biomedical Engineering , Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
authors.forename: M Manuela
authors.initials: MM
authors.lastname: Brás
authors.affiliation: Laboratory of Biomaterials and Specialty Polymers, LBPS-CSPBAT CNRS UMR 7244, Institut Galilée, Université Paris 13 , 93430 Villetaneuse, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Migonney
authors.affiliation: New Jersey Center for Biomaterials, Rutgers University , 145 Bevier Road, Piscataway, New Jersey 08854, United States.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kohn
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D018786:Bone Substitutes / Q000378:metabolism; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D011137:Polystyrenes / Q000737:chemistry / Q000378:metabolism; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D018786:Bone Substitutes; D011137:Polystyrenes; D012710:Serum Albumin, Bovine; D006046:Gold
keywords: bovine serum albumin; collagen type I; competitive adsorption; fibronectin; hydrophobic surfaces; poly(NaSS) surfaces