title: Mangiferin attenuates DSS colitis in mice: Molecular docking and in vivo approach.
abstract: Inflammation, oxidative stress and altered mucosal barrier permeability are potential etiopathological or triggering factors for inflammatory bowel disease (IBD). In this study, the therapeutic potential of Mangiferin was investigated in vivo in mouse model of colitis and also attempts were made to understand mechanistic insights of Mangiferin in IBD. In present study, colitis was induced by administration of 5% DSS for 11 days, followed by 3 days of DSS free period. On day 14, animals were sacrificed and colon tissues were taken for biochemical and histological analysis. Therapeutic treatment with Mangiferin after colitis induction (i.e. day 5) ameliorated symptoms of colitis (presence of blood in stools, body weight loss and diarrhea) as evidenced by reduced DAI score, attenuated the levels of catalase (CAT), reduced glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO). It also decreased the colonic pro-inflammatory mediators tumor necrosis factor (TNF-α), interleukin 1β (IL-1β) levels, matrix metalloproteinase-9 (MMP-9) activity and histopathological score. Molecular docking of Mangiferin against TNF-α and MMP-9 was evaluated using GLIDE software. Mangiferin demonstrated the glide score of -8.04 kcal/mol for TNF-α and -9.97 kcal/mol for MMP-9, which indicated its binding potential with TNF-α and MMP-9. In conclusion, Mangiferin reduces colonic damage in a murine model of colitis, alleviates the oxidative and inflammatory events partly through directly influencing the activity of TNF-α and MMP-9 and therefore might have therapeutic usefulness in the management of inflammatory bowel disease.
authors.affiliation: Department of Pharmacology, Torrent Pharmaceuticals Ltd, Gandhinagar, Gujarat, India; Department of Pharmacology, School of Pharmacy, RK University, Rajkot, India. Electronic address: sahilsomani_87@yahoo.co.in.
authors.forename: Sahil
authors.initials: S
authors.lastname: Somani
authors.affiliation: Department of Pharmacology, Torrent Pharmaceuticals Ltd, Gandhinagar, Gujarat, India. Electronic address: shitalkumarzambad@torrentpharma.com.
authors.forename: Shitalkumar
authors.initials: S
authors.lastname: Zambad
authors.affiliation: Department of Pharmacology, B K Mody Government Pharmacy College, Rajkot, India. Electronic address: ketanmodipharmacy@gmail.com.
authors.forename: Ketan
authors.initials: K
authors.lastname: Modi
mesh_terms: D000818:Animals; D001665:Binding Sites; D001835:Body Weight / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D062105:Molecular Docking Simulation; D017434:Protein Structure, Tertiary; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000378:metabolism; D044004:Xanthones / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D044004:Xanthones; C013592:mangiferin; D008315:Malondialdehyde; D016264:Dextran Sulfate; D002374:Catalase; D013482:Superoxide Dismutase; D020780:Matrix Metalloproteinase 9; D005978:Glutathione
keywords: Colitis; Dextran sulfate sodium; Mangiferin; Matrix metalloproteinase-9