title: Comparative Immunophenotypic Characteristics, Proliferative Features, and Osteogenic Differentiation of Stem Cells Isolated from Human Permanent and Deciduous Teeth with Bone Marrow.
abstract: To find out differences and similarities in phenotypic, proliferative, and trans-differentiation properties of stem cells isolated from pulp of deciduous (SHEDs) and permanent (DPSCs) teeth with human bone marrow stem cells (BMSCs), we examined the expression of mesenchymal and embryonic stem cell markers in relation to the proliferation and osteogenic differentiation potentials of these cells. In this way, after isolating SHEDs, DPSCs, and BMSCs, cell proliferation was evaluated and population doubling time was calculated accordingly. Expression patterns of mesenchymal, hematopoietic, and embryonic stem cell markers were assessed followed by examining differentiation potential toward osseous tissue through alizarin red staining and qRT-PCR. Based on the results, the proliferation rates of SHEDs and DPSCs were significantly higher than that of BMSCs (P < 0.0001). High expression of mesenchymal stem cell markers and weak expression of hematopoietic markers were observed in all the three groups. The mean expression of OCT-4 was significantly higher in SHEDs and DPSCs (P = 0.028), while the expression of SSEA-4 was lower (P = 0.006) compared to BMSCs. Osteogenic differentiation potential of SHEDs was greater than DPSCs; however, it was lower than that of BMSCs. Conclusively, the distinctive immunophenotyping, proliferation rate, and differentiation pattern of SHEDs and DPSCs discriminate these cells from BMSCs. Furthermore, dissimilarity in differentiation potential is evidence implying that SHEDs might be more primitive stem cell population compared to DPSCs.
authors.affiliation: Department of Dental Biomaterials, School of Dentistry/Dental Research Centre, Dentistry Research Institute, Tehran University of Medical Sciences, Ghods St., Keshavarz Blvd, P.O. Box: 14155-5583, Tehran, 14174, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Aghajani
authors.affiliation: Department of Dental Biomaterials, School of Dentistry/Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, North Karegar St, Tehran, Iran.
authors.forename: Tabassom
authors.initials: T
authors.lastname: Hooshmand
authors.affiliation: Reproductive Biotechnology Research Centre, Avicenna Research Institute, ACECR, P.O. Box 19615-1177, Tehran, Islamic Republic of Iran.
authors.forename: Manijeh
authors.initials: M
authors.lastname: Khanmohammadi
authors.affiliation: Reproductive Biotechnology Research Centre, Avicenna Research Institute, ACECR, P.O. Box 19615-1177, Tehran, Islamic Republic of Iran.
authors.forename: Sayeh
authors.initials: S
authors.lastname: Khanjani
authors.affiliation: Reproductive Biotechnology Research Centre, Avicenna Research Institute, ACECR, P.O. Box 19615-1177, Tehran, Islamic Republic of Iran.
authors.forename: Haleh
authors.initials: H
authors.lastname: Edalatkhah
authors.affiliation: Nanobiotechnology Research Centre, Avicenna Research Institute, ACECR, Tehran, Iran.
authors.forename: Amir-Hassan
authors.initials: AH
authors.lastname: Zarnani
authors.affiliation: Reproductive Biotechnology Research Centre, Avicenna Research Institute, ACECR, P.O. Box 19615-1177, Tehran, Islamic Republic of Iran. kazemnejad_s@yahoo.com.
authors.forename: Somaieh
authors.initials: S
authors.lastname: Kazemnejad
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D003782:Dental Pulp / Q000166:cytology*; D019229:Dentition, Permanent; D005786:Gene Expression Regulation; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010012:Osteogenesis; D055746:Stage-Specific Embryonic Antigens / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology*; D014094:Tooth, Deciduous / Q000166:cytology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4
keywords: Dental pulp stem cell; Immunophenotyping; Mesenchymal stem cells; Osteogenic Differentiation; Stem cells of human exfoliated deciduous teeth