title: Serogenotyping and antimicrobial susceptibility testing of Salmonella spp. isolated from retail meat samples in Lagos, Nigeria.
abstract: Microarray-based serogenotyping, antimicrobial susceptibility tests and the detection of relevant resistance genes were performed on isolates of Salmonella spp. from retail meat samples obtained in Lagos, Nigeria. Out of 151 meat samples, 33 Salmonella isolates were obtained. Nine different Salmonella serovars (S. Amoutive, S. Bargny, S. Drac, S. Ealing, S. Urbana, S. Hadar, S. Nyborg, S. Anatum and S. Havana) were identified by microarray-based serogenotyping and confirmed afterwards using classical serotyping. Antibiotic susceptibility tests with 17 antibiotics showed that almost all isolates were fully susceptible to this panel. The results of this study indicated a high prevalence of Salmonella in retail meat, the presence of some previously rather rarely described Serovars in retail meat samples from Lagos, and a need to monitor for Salmonella and their antibiotic resistance determinants. The microarray-based system used herein proved to be perfectly suited as epidemiological tool to replace classical serotyping.
authors.affiliation: Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria. Electronic address: stellaismith@yahoo.com.
authors.forename: Stella
authors.initials: S
authors.lastname: Smith
authors.affiliation: Alere Technologies GmbH, Jena, Germany; InfectoGnostics Research Campus, Jena, Germany. Electronic address: sascha.braun@clondiag.com.
authors.forename: Sascha
authors.initials: S
authors.lastname: Braun
authors.affiliation: College of Medicine, University of Lagos, Lagos, Nigeria. Electronic address: akintimehinopeoluwas@yahoo.com.
authors.forename: Faith
authors.initials: F
authors.lastname: Akintimehin
authors.affiliation: Public Health Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria. Electronic address: wuratoun@yahoo.co.uk.
authors.forename: Toun
authors.initials: T
authors.lastname: Fesobi
authors.affiliation: Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria. Electronic address: mosesbamidele@yahoo.com.
authors.forename: Moses
authors.initials: M
authors.lastname: Bamidele
authors.affiliation: College of Medicine, University of Lagos, Lagos, Nigeria. Electronic address: aocoker@hotmail.com.
authors.forename: Akitoye
authors.initials: A
authors.lastname: Coker
authors.affiliation: Alere Technologies GmbH, Jena, Germany; InfectoGnostics Research Campus, Jena, Germany; TU Dresden, Institute for Medical Microbiology and Hygiene, Dresden, Germany. Electronic address: stefan.monecke@clondiag.com.
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
authors.affiliation: Alere Technologies GmbH, Jena, Germany; InfectoGnostics Research Campus, Jena, Germany. Electronic address: ralf@clondiag.com.
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D060005:Genotyping Techniques / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009549:Nigeria; D012475:Salmonella / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D012703:Serotyping / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents
keywords: Africa; DNA-based microarray; Genotyping; Retail meat; Salmonella; Serogenotyping; Serotyping