title: A comparative cytogenetic study of Drosophila parasitoids (Hymenoptera, Figitidae) using DNA-binding fluorochromes and FISH with 45S rDNA probe.
abstract: Karyotypes of Leptopilina boulardi (Barbotin, Carton et Keiner-Pillault, 1979) (n = 9), L. heterotoma (Thomson, 1862) (n = 10), L. victoriae Nordlander, 1980 (n = 10) and Ganaspis xanthopoda (Ashmead, 1896) (n = 9) (Hymenoptera, Figitidae) were studied using DNA-binding ligands with different base specificity [propidium iodide (PI), chromomycin A3 (CMA3) and 4',6-diamidino-2-phenylindole (DAPI)], and fluorescence in situ hybridization (FISH) with a 45S rDNA probe. Fluorochrome staining was similar between the different fluorochromes, except for a single CMA3- and PI-positive and DAPI-negative band per haploid karyotype of each species. FISH with 45S rDNA probe detected a single rDNA site in place of the bright CMA3-positive band, thus identifying the nucleolus organizing region (NOR). Chromosomal locations of NORs were similar for both L. heterotoma and L. victoriae, but strongly differed in L. boulardi as well as in G. xanthopoda. Phylogenetic aspects of NOR localization in all studied species are briefly discussed.
authors.affiliation: Botanical Garden, Moscow State University, Moscow, Russia, 119234. vegokhman@hotmail.com.
authors.forename: Vladimir E
authors.initials: VE
authors.lastname: Gokhman
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia, 119991.
authors.forename: Nadezhda L
authors.initials: NL
authors.lastname: Bolsheva
authors.affiliation: Biology Department MR526, The City College of the City University of New York, 138th Street and Convent Avenue, New York, NY, 10031, USA.
authors.forename: Shubha
authors.initials: S
authors.lastname: Govind
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia, 119991.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Muravenko
mesh_terms: D000818:Animals; D003582:Cytogenetics; D015342:DNA Probes; D005456:Fluorescent Dyes; D006927:Hymenoptera / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D059785:Karyotype; D012335:RNA, Ribosomal / Q000235:genetics*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D012335:RNA, Ribosomal; C088179:RNA, ribosomal, 45S
keywords: 45S rDNA; DNA-binding fluorochromes; FISH; Figitidae; Hymenoptera; Karyotypes