title: DNA stability of Chlamydia trachomatis and Neisseria gonorrhoeae in urine.
abstract: We evaluated the DNA stability of Chlamydia trachomatis and Neisseria gonorrhoeae in 55 urine samples. Crossing threshold (Ct) values were highly similar after 3 to 14 days at room temperature (+0.002, P = 0.99). Consequently, it does not seem necessary to transfer urine specimens into a transport medium in less than 24 hours as recommended by manufacturers.
authors.affiliation: Université de Lille, UFR Médecine, F-59000 Lille, France; CHU Lille, Institut de Microbiologie, F-59000 Lille, France. Electronic address: remi.leguern@gmail.com.
authors.forename: Rémi
authors.initials: R
authors.lastname: Le Guern
authors.affiliation: CHU Lille, Institut de Microbiologie, F-59000 Lille, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Miaux
authors.affiliation: CHU Lille, Institut de Microbiologie, F-59000 Lille, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Pischedda
authors.affiliation: CHU Lille, Institut de Microbiologie, F-59000 Lille, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Herwegh
authors.affiliation: Université de Lille, UFR Médecine, F-59000 Lille, France; CHU Lille, Institut de Microbiologie, F-59000 Lille, France.
authors.forename: René
authors.initials: R
authors.lastname: Courcol
mesh_terms: D002692:Chlamydia trachomatis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000175:diagnosis*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014556:Urine / Q000737:chemistry / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: Chlamydia trachomatis; Cobas 4800 CT/NG; DNA stability; Neisseria gonorrhoeae; Sexually transmitted infections