title: Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae).
abstract: OBJECTIVE
  Microsatellite markers can be used to evaluate population structure and genetic diversity in native populations of Indigofera pseudotinctoria (Fabaceae) and assess genetic disturbance caused by nonnative plants of the same species.
  
  
  RESULTS
  We developed 14 markers for I. pseudotinctoria using next-generation sequencing and applied them to test two native populations, totaling 77 individuals, and a transplanted population, imported from a foreign country, of 17 individuals. The mean number of alleles was 3.310, observed heterozygosity was 0.242, and expected heterozygosity was 0.346. The fixation index in the transplanted population was 0.469, which was higher than in the native populations (0.154 and 0.158). In addition, the transplanted population contains one allele that is not shared by the native population.
  
  
  CONCLUSIONS
  Microsatellite markers can be useful for evaluating genetic diversity within and between populations and for studying population genetics in I. pseudotinctoria and related species.
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Otao
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan.
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Uehara
keywords: 454 next-generation sequencing; Fabaceae; Indigofera pseudotinctoria; invasive species; microsatellites