title: Short hairpin rna targeting insulin-like growth fator binding protein-3 restores the bioavailability of insulin-like growth factor-1 in diabetic rats.
abstract: OBJECTIVE
  To investigate whether intracavernosal injection of short hairpin RNA for IGFBP-3 could improve erectile function in streptozotocin-induced diabetic rats.
  
  
  METHODS
  After 12 weeks of IGFBP-3 short hairpin RNA injection treatment, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 and IGF-1 at mRNA and protein levels were detected by quantitative real-time PCR analysis and Western blot, respectively. The concentration of cavernous cyclic guanosine monophosphate was detected by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  At 12 weeks after intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic group (P<0.05). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. At the same time, cavernous IGF-1 expression was significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic group (P<0.01). Cavernous cyclic guanosine monophosphate concentration was significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic group (P<0.01).
  
  
  CONCLUSIONS
  Gene transfer of IGFBP-3 shRNA could improve erectile function via the restoration of cavernous IGF-1 bioavailability and an increase of cavernous cGMP concentration in the pathogenesis of erectile dysfunction in streptozotocin-induced diabetic rats.
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Zhang-Yan
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Zhong
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Cheng
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Chang-Mao
authors.initials: CM
authors.lastname: Liu
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xing
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Sun
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Liu
authors.affiliation: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D001682:Biological Availability; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D007172:Erectile Dysfunction / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology*; D007267:Injections; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000493:pharmacokinetics*; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000187:drug effects*; D008297:Male; D010413:Penis / Q000187:drug effects*; D034741:RNA, Small Interfering / Q000493:pharmacokinetics*; D011897:Random Allocation; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013311:Streptozocin
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D034741:RNA, Small Interfering; C548904:insulin-like growth factor-1, rat; D013311:Streptozocin; D007334:Insulin-Like Growth Factor I