title: Laminin-111 Peptides Conjugated to Fibrin Hydrogels Promote Formation of Lumen Containing Parotid Gland Cell Clusters.
abstract: Previous studies showed that mouse submandibular gland cells form three-dimensional structures when grown on Laminin-111 gels. The use of Laminin-111 for tissue bioengineering is complicated due to its lack of purity. By contrast, the use of synthetic peptides derived from Laminin-111 is beneficial due to their high purity and easy manipulation. Two Laminin-111 peptides have been identified for salivary cells: the A99 peptide corresponding to the α1 chain from Laminin-111 and the YIGSR peptide corresponding to the β1 chain from Laminin-111, which are important for cell adhesion and migration. We created three-dimensional salivary cell clusters using a modified fibrin hydrogel matrix containing immobilized Laminin-111 peptides. Results indicate that the YIGSR peptide improved morphology and lumen formation in rat parotid Par-C10 cells as compared to cells grown on unmodified fibrin hydrogel. Moreover, a combination of both peptides not only allowed the formation of functional three-dimensional salivary cell clusters but also increased attachment and number of cell clusters. In summary, we demonstrated that fibrin hydrogel decorated with Laminin-111 peptides supports attachment and differentiation of salivary gland cell clusters with mature lumens.
authors.affiliation: School of Dentistry, ‡Department of Bioengineering, The University of Utah , Salt Lake City, Utah 84108, United States.|Department of Chemical and Biological Engineering, ∥Department of Biomedical Engineering, School of Engineering and Applied Sciences, ⊥Center of Bioinformatics and Life Sciences, University at Buffalo, The State University of New York , Buffalo, New York 14260, United States.
authors.forename: Kihoon
authors.initials: K
authors.lastname: Nam
authors.affiliation: School of Dentistry, ‡Department of Bioengineering, The University of Utah , Salt Lake City, Utah 84108, United States.|Department of Chemical and Biological Engineering, ∥Department of Biomedical Engineering, School of Engineering and Applied Sciences, ⊥Center of Bioinformatics and Life Sciences, University at Buffalo, The State University of New York , Buffalo, New York 14260, United States.
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Jones
authors.affiliation: School of Dentistry, ‡Department of Bioengineering, The University of Utah , Salt Lake City, Utah 84108, United States.|Department of Chemical and Biological Engineering, ∥Department of Biomedical Engineering, School of Engineering and Applied Sciences, ⊥Center of Bioinformatics and Life Sciences, University at Buffalo, The State University of New York , Buffalo, New York 14260, United States.
authors.forename: Pedro
authors.initials: P
authors.lastname: Lei
authors.affiliation: School of Dentistry, ‡Department of Bioengineering, The University of Utah , Salt Lake City, Utah 84108, United States.|Department of Chemical and Biological Engineering, ∥Department of Biomedical Engineering, School of Engineering and Applied Sciences, ⊥Center of Bioinformatics and Life Sciences, University at Buffalo, The State University of New York , Buffalo, New York 14260, United States.
authors.forename: Stelios T
authors.initials: ST
authors.lastname: Andreadis
authors.affiliation: School of Dentistry, ‡Department of Bioengineering, The University of Utah , Salt Lake City, Utah 84108, United States.|Department of Chemical and Biological Engineering, ∥Department of Biomedical Engineering, School of Engineering and Applied Sciences, ⊥Center of Bioinformatics and Life Sciences, University at Buffalo, The State University of New York , Buffalo, New York 14260, United States.
authors.forename: Olga J
authors.initials: OJ
authors.lastname: Baker
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D005337:Fibrin / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism; D010306:Parotid Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D051381:Rats
chemical_list: D020100:Hydrogels; D007797:Laminin; D010446:Peptide Fragments; D005337:Fibrin