title: Multiresidue method for simultaneous analysis of aflatoxin M1, avermectins, organophosphate pesticides and milbemycin in milk by ultra-performance liquid chromatography coupled to tandem mass spectrometry.
abstract: A method developed for the simultaneous analysis of aflatoxin M1, abamectin, doramectin, eprinomectin, ivermectin, moxidectin, acephate, azinphos-ethyl, azinphos-methyl, diazinon, methamidophos, methidathion, mevinphos, pirimiphos-ethyl and pirimiphos-methyl in whole raw milk, based on the QuEChERS method for extraction and clean-up, with detection and quantification by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) is described. The method was validated according to parameters of the Analytical Quality Assurance Manual from the Brazilian Ministry of Agriculture and Commission Decision 2002/657/EC, and proved suitable for analysis of these analytes within the proposed working range, with recovery values between 77% and 110%, a standard deviation lower than 20%, limits of detection between 0.05 and 0.99 µg l(-)(1), and limits of quantification between 0.15 and 1.98 µg l(-1). Samples from animals treated with abamectin, doramectin, ivermectin and diazinon were analysed by the validated method. Residues of aflatoxin M1 were also found in field samples at levels below the established maximum residue limit.
authors.affiliation: a Laboratório de Resíduos e Contaminantes , Embrapa Agroindústria de Alimentos , Rio de Janeiro , Brazil.
authors.forename: Marianna Ramos
authors.initials: MR
authors.lastname: Dos Anjos
authors.affiliation: a Laboratório de Resíduos e Contaminantes , Embrapa Agroindústria de Alimentos , Rio de Janeiro , Brazil.
authors.forename: Izabela Miranda de
authors.initials: IM
authors.lastname: Castro
authors.affiliation: a Laboratório de Resíduos e Contaminantes , Embrapa Agroindústria de Alimentos , Rio de Janeiro , Brazil.
authors.forename: Maria de Lourdes Mendes de
authors.initials: Mde L
authors.lastname: Souza
authors.affiliation: b Instituto de Química, LAB-RES/LADETEC , Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Virgínia Verônica
authors.initials: VV
authors.lastname: de Lima
authors.affiliation: b Instituto de Química, LAB-RES/LADETEC , Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Francisco Radler
authors.initials: FR
authors.lastname: de Aquino-Neto
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007559:Ivermectin / Q000031:analogs & derivatives / Q000032:analysis; D018942:Macrolides / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D010755:Organophosphates / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D018942:Macrolides; D010755:Organophosphates; D010573:Pesticide Residues; D016607:Aflatoxin M1; D007559:Ivermectin; C019264:avermectin; C027837:moxidectin
keywords: QuEChERS; UPLC-MS/MS; aflatoxin M1; macrocyclic lactones; milk; organophosphate pesticides