title: Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil.
abstract: The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, Brazil. joanamascarenhas@iec.pa.gov.br.
authors.forename: Joana D P
authors.initials: JD
authors.lastname: Mascarenhas
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, Brazil.
authors.forename: Delana A M
authors.initials: DA
authors.lastname: Bezerra
authors.affiliation: Laboratório Nacional Agropecuário (LANAGRO), Ministério da Agricultura, Belém, Brazil.
authors.forename: René R
authors.initials: RR
authors.lastname: Silva
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, Brazil.
authors.forename: Mayara J M
authors.initials: MJ
authors.lastname: Silva
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, Brazil.
authors.forename: Edivaldo C Sousa
authors.initials: EC
authors.lastname: Júnior
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, Brazil.
authors.forename: Luana S
authors.initials: LS
authors.lastname: Soares
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000378:metabolism; D001938:Brazil; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens; D005243:Feces / Q000821:virology*; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C061332:VP6 protein, Rotavirus
keywords: Broiler; RT-PCR; Rotavirus F; Rotavirus G