title: Regulation of allergic inflammation by the ectoenzyme E-NPP3 (CD203c) on basophils and mast cells.
abstract: Adenosine 5'-triphosphate (ATP) is released from dying or damaged cells, as well as from activated cells. Once secreted, extracellular ATP induces several immune responses via P2X and P2Y receptors. Basophils and mast cells release ATP upon FcεRI-crosslinking, and ATP activates basophils and mast cells in an autocrine manner. Nucleotide-converting ectoenzymes, such as E-NTPD1, E-NTPD7, and E-NPP3, inhibit ATP-dependent immune responses by hydrolyzing ATP, thereby contributing to immune response regulation. E-NPP3 is a well-known activation marker for human basophils. E-NPP3's physiologic function has recently been disclosed in mice. E-NPP3 is rapidly induced on basophils and mast cells after FcεRI-crosslinking and hydrolyzes extracellular ATP on cell surfaces to prevent ATP-dependent excess activation of basophils and mast cells. In the absence of E-NPP3, basophils and mast cells are overactivated and mice suffer from severe chronic allergic inflammation. Thus, the ATP-hydrolyzing ectoenzymes E-NPP3 has a nonnegligible role in the regulation of basophil- and mast cell-mediated allergic responses.
authors.affiliation: Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka, 565-0871, Japan.
authors.forename: Shih Han
authors.initials: SH
authors.lastname: Tsai
authors.affiliation: Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka, 565-0871, Japan. ktakeda@ongene.med.osaka-u.ac.jp.|Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo, 100-0004, Japan. ktakeda@ongene.med.osaka-u.ac.jp.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Takeda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001491:Basophils / Q000276:immunology* / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism*; D007527:Isoenzymes; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011485:Protein Binding; D011755:Pyrophosphatases / Q000378:metabolism*; D011983:Receptors, Purinergic / Q000378:metabolism
chemical_list: C427944:ENPP3 protein, human; D007527:Isoenzymes; D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases
keywords: ATP; Allergy; Basophil; E-NPP3; Mast cell