title: Molecular characterisation and antifungal susceptibility of clinical Trichosporon isolates in India.
abstract: In Asian countries, Trichosporon infection is a well-known disease in Japan. In India, the infection is increasingly recognised. The study was conducted to characterise the clinical Trichosporon isolates from India by phenotypic and molecular techniques. A total of 31 Trichosporon clinical isolates, recovered from patients of 14 hospitals across India were sequenced (ITS and IGS1 regions of rDNA). In vitro drug susceptibility testing of the isolates was performed against amphotericin-B, fluconazole, itraconazole, voriconazole and posaconazole. IGS1, rather than ITS sequences, correctly identified the isolates: Trichosporon asahii, 20; Trichosporon ovoides, 3; Trichosporon inkin, 2; Trichosporon asteroides, 1; Trichosporon mucoides, 1; Trichosporon loubieri, 1; Trichosporon debeurmannianum, 1; and Trichosporon dermatis, 1. Trichosporon asahii genotype III was the most common type, followed by genotype I and VII. Both these targets did not help to identify one Trichosporon to the species level. Trichosporon debeurmannianum, T. dermatis and T. asteroides were isolated for the first time from a human disease in India. The minimum inhibitory concentrations for voriconazole and posaconazole were within effective range. The study highlights the presence of wide range of Trichosporon species causing infection in India. Voriconazole or posaconazole may be the better drugs to treat such patients.
authors.affiliation: Department of Microbiology, Jawaharlal Nehru Medical College and Associate Groups of Hospitals, Ajmer, India.
authors.forename: Vijaylatha
authors.initials: V
authors.lastname: Rastogi
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
authors.forename: Prasanna
authors.initials: P
authors.lastname: Honnavar
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
authors.forename: Shivaprakash M
authors.initials: SM
authors.lastname: Rudramurthy
authors.affiliation: Department of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, India.
authors.forename: Umabala
authors.initials: U
authors.lastname: Pamidi
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
authors.forename: Anup
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
authors.forename: Arunaloke
authors.initials: A
authors.lastname: Chakrabarti
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D017964:Itraconazole / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D010641:Phenotype; D017422:Sequence Analysis, DNA; D014230:Triazoles / Q000494:pharmacology; D014250:Trichosporon / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D060586:Trichosporonosis / Q000453:epidemiology / Q000382:microbiology; D065819:Voriconazole / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D014230:Triazoles; D017964:Itraconazole; C101425:posaconazole; D000666:Amphotericin B; D015725:Fluconazole; D065819:Voriconazole
keywords: Trichosporon; antifungal susceptibility; genotyping; identification; systemic infection