title: Automated hollow-fiber liquid-phase microextraction followed by liquid chromatography with mass spectrometry for the determination of benzodiazepine drugs in biological samples.
abstract: In this study, two-phase hollow-fiber liquid-phase microextraction and three-phase hollow-fiber liquid-phase microextraction based on two immiscible organic solvents were compared for extraction of oxazepam and Lorazepam. Separations were performed on a liquid chromatography with mass spectrometry instrument. Under optimal conditions, three-phase hollow-fiber liquid-phase microextraction based on two immiscible organic solvents has a better extraction efficiency. In a urine sample, for three-phase hollow fiber liquid-phase microextraction based on two immiscible organic solvents, the calibration curves were found to be linear in the range of 0.6-200 and 0.9-200 μg L(-1) and the limits of detection were 0.2 and 0.3 μg L(-1) for oxazepam and lorazepam, respectively. For two-phase hollow fiber liquid-phase microextraction, the calibration curves were found to be linear in the range of 1-200 and 1.5-200 μg L(-1) and the limits of detection were 0.3 and 0.5 μg L(-1) for oxazepam and lorazepam, respectively. In a urine sample, for three-phase hollow-fiber-based liquid-phase microextraction based on two immiscible organic solvents, relative standard deviations in the range of 4.2-4.5% and preconcentration factors in the range of 70-180 were obtained for oxazepam and lorazepam, respectively. Also for the two-phase hollow-fiber liquid-phase microextraction, preconcentration factors in the range of 101-257 were obtained for oxazepam and lorazepam, respectively.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Nazaripour
authors.affiliation: Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Yadollah
authors.initials: Y
authors.lastname: Yamini
authors.affiliation: Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Ebrahimpour
authors.affiliation: Nuclear Science and Technology Research Institute, AEOI, Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Fasihi
mesh_terms: D001331:Automation; D001569:Benzodiazepines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D059627:Liquid Phase Microextraction; D013058:Mass Spectrometry; D016062:Porosity
chemical_list: D001569:Benzodiazepines
keywords: Automated extraction; Benzodiazepines; Hollow fiber; Liquid chromatography; Microextraction