title: Dietary Supplementation of Ginger and Turmeric Rhizomes Modulates Platelets Ectonucleotidase and Adenosine Deaminase Activities in Normotensive and Hypertensive Rats.
abstract: Hypertension is associated with platelet alterations that could contribute to the development of cardiovascular complications. Several studies have reported antiplatelet aggregation properties of ginger (Zingiber officinale) and turmeric (Curcuma longa) with limited scientific basis. Hence, this study assessed the effect of dietary supplementation of these rhizomes on platelet ectonucleotidase and adenosine deaminase (ADA) activities in Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME) induced hypertensive rats. Animals were divided into seven groups (n = 10): normotensive control rats; induced (l-NAME hypertensive) rats; hypertensive rats treated with atenolol (10 mg/kg/day); normotensive and hypertensive rats treated with 4% supplementation of turmeric or ginger, respectively. After 14 days of pre-treatment, the animals were induced with hypertension by oral administration of l-NAME (40 mg/kg/day). The results revealed a significant (p < 0.05) increase in platelet ADA activity and ATP hydrolysis with a concomitant decrease in ADP and AMP hydrolysis of l-NAME hypertensive rats when compared with the control. However, dietary supplementation with turmeric or ginger efficiently prevented these alterations by modulating the hydrolysis of ATP, ADP and AMP with a concomitant decrease in ADA activity. Thus, these activities could suggest some possible mechanism of the rhizomes against hypertension-derived complications associated to platelet hyperactivity. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology Akure, Private Mail Bag 704, Akure, 340001, Nigeria.|Department of Biochemistry, Afe Babalola University, Private Mail Bag 5454, Ado-Ekiti, Nigeria.|Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Ayodele Jacob
authors.initials: AJ
authors.lastname: Akinyemi
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Gustavo Roberto
authors.initials: GR
authors.lastname: Thomé
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Jucimara
authors.initials: J
authors.lastname: Baldissarelli
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Lizielle Souza
authors.initials: LS
authors.lastname: de Oliveira
authors.affiliation: Health Basic Sciences Institute, Department of Physiology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Jeferson Ferraz
authors.initials: JF
authors.lastname: Goularte
authors.affiliation: Health Basic Sciences Institute, Department of Physiology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Adriane
authors.initials: A
authors.lastname: Belló-Klein
authors.affiliation: Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology Akure, Private Mail Bag 704, Akure, 340001, Nigeria.
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D001794:Blood Pressure / Q000187:drug effects; D030024:Curcuma; D019587:Dietary Supplements; D020939:Zingiber officinale; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome
chemical_list: D000243:Adenosine Deaminase; D000251:Adenosine Triphosphatases; C027201:ectoATPase; D019331:NG-Nitroarginine Methyl Ester
keywords: ADA; Curcuma longa; Zingiber officinale; ectonucleotidases; hypertension; platelet