title: [Effect of Magnolol on Proliferation and Apoptosis of HL-60 Cells and Its Molecular Mechanism].
abstract: OBJECTIVE
  To investigate the effect of magnolol on proliferation and apoptosis of HL-60 cells and its mechanism.
  
  
  METHODS
  MTT assay was used to measure the proliferation of HL-60 cells after treatment with different concentration of magnolol (5, 10, 20, 40, 80 and 160 µg/ml). The morphological changes of HL-60 cells were examined by light microscopy, and DAPI staining was performed to observe the nuclear morphology of HL-60 cells. The early cell apoptosis was detected by flow cytometry with Annexin V-FITC/PI double-staining. RT-PCR was carried out to examine the mRNA expression of BAX and BCL-2. Western blot was performed to detect the protein expression of caspase family.
  
  
  RESULTS
  The magnolol inhibited HL-60 cell proliferation, and the inhibitory rate of cell proliferation increased significantly in a dose- and time- dependent manner (P < 0.05). HL-60 cells became small, even apoptotic bodies appeared after treatment with magnolol. In addition, nuclear condensation or fragmentation could be observed, which is the typical morphological features of apoptosis. When HL-60 cells were treated with 40 µg/ml of magnolol for 24 h, the ratio of early apoptotic cells reached to (11.7 ± 2.4) %, which was significant different from control (1.4 ± 1.1) % (P < 0.05). RT-PCR results showed that treatment of HL-60 cells with magnolol up-regulated the expression of BAX, whereas down-regulated the expression of BCL-2. Western blot results showed that the cleavages of caspase-3, -8 and -9 were significantly enhanced by magnolol.
  
  
  CONCLUSIONS
  The magnolol can significantly inhibit the proliferation of HL-60 cells and induce the apoptosis of HL-60 cells, which may occur through up-regulation of BAX, down-regulation of BCL-2 and the activation of caspases.
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.|School of Medicine and Life Sciences, Ji'nan University, Jinan 250200, Shandong Province, China.|The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250062, Shangdong Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Fang
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.|School of Medicine and Life Sciences, Ji'nan University, Jinan 250200, Shandong Province, China.
authors.forename: Xiao-Fen
authors.initials: XF
authors.lastname: Yuan
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.|School of Medicine and Life Sciences, Ji'nan University, Jinan 250200, Shandong Province, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liao
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Song
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China. E-mail: renxia2002@sohu.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Ren
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China. E-mail: jianggsh@hotmail.com.
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation; D005434:Flow Cytometry; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D001713:Biphenyl Compounds; D017705:Lignans; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C005498:magnolol; D020169:Caspases