title: A novel biosensor based on the direct electrochemistry of horseradish peroxidase immobilized in the three-dimensional flower-like Bi2WO6 microspheres.
abstract: Three-dimensional flower-like Bi2WO6 microspheres (3D-Bi2WO6 MSs) have been synthesized through a simple hydrothermal method. The morphology and structure of 3D-Bi2WO6 MSs were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The 3D-Bi2WO6 MSs subsequently were used to immobilize horseradish peroxidase (HRP) and fabricate a mediator-free biosensor for the detection of H2O2. Spectroscopic and electrochemical results reveal that 3D-Bi2WO6 MSs constitute an excellent immobilization matrix with biocompatibility for enzymes. Meanwhile, due to unique morphology of the flower-like microspheres, the direct electron transfer of HRP is facilitated and the prepared biosensors display good performances for the detection of H2O2 with a wide linear range, including two linear sections: 0.5-100μM (R(2)=0.9983) and 100-250μM (R(2)=0.9981), as well as an extremely low method detection limit of 0.18μM.
authors.affiliation: School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021. PR China. Electronic address: liuhui@sust.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021. PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.affiliation: School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021. PR China.
authors.forename: Congyue
authors.initials: C
authors.lastname: Duan
authors.affiliation: School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021. PR China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021. PR China.
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001729:Bismuth / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D008863:Microspheres; D017973:Tungsten Compounds / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D017973:Tungsten Compounds; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C021101:tungstic(VI) acid; D001729:Bismuth
keywords: Bi(2)WO(6); Biosensor; Enzyme immobilization; Hydrogen peroxide; Three-dimensional flower-like microspheres