title: Imprinted gene OsFIE1 modulates rice seed development by influencing nutrient metabolism and modifying genome H3K27me3.
abstract: Imprinted Polycomb group (PcG) genes play a critical role in seed development in Arabidopsis. However, the role of the imprinted gene in cereal plants remains obscure. Here, a transgenic approach was conducted to study the function of the imprinted gene Oryza sativa Fertilization-Independent Endosperm 1 (OsFIE1) during seed development in rice (Oryza sativa ssp. japonica 'ZhongHua11'). RNAi of OsFIE1 and homozygous T-DNA insertion mutant osfie1 led to smaller seeds, delayed embryo development, smaller aleurone layer cells, and decreased seed set rate. OsFIE1 was specifically expressed in endosperm, and mRNA of OsFIE1 was also enriched in the inner seed coat together with the corresponding PcG members OsiEZ1 and OsCLF. Meanwhile, the contents of seed storage proteins and Ile, Leu, and Val were decreased, accompanied by the down-regulation of multiple transcription factors, storage protein synthesis and amino acid metabolism-related genes in OsFIE1-RNAi lines and osfie1. Western blot analysis showed that the complex OsFIE1-PcG in endosperm regulated the expression of target genes by genome H3K27me3 modification. We conclude that the OsFIE1-PcG complex, which was enriched in the inner seed coat and endosperm linked the development of embryo and endosperm by influencing transcription factors and nutrient metabolism and induced a highly differential effect when compared with the OsFIE2-PcG complex.
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.|Key Laboratory of Plant Physiology and Development Regulation, Guizhou Normal University, Guiyang, 550001, China.|School of Life Science, Guizhou Normal University, Guiyang, 550001, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zhanhua
authors.initials: Z
authors.lastname: Lu
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Koulong
authors.initials: K
authors.lastname: Xie
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Dongying
authors.initials: D
authors.lastname: Wang
authors.affiliation: CSIRO Plant Industry, PO Box 1600, Canberra, ACT, 2601, Australia.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. yaojlmy@mail.hzau.edu.cn.
authors.forename: Jialing
authors.initials: J
authors.lastname: Yao
mesh_terms: D056625:Endosperm / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins
keywords: Oryza sativa; PcG; aleurone layer; imprinted gene; seed development