title: Glucocorticoid receptor mediated the propofol self-administration by dopamine D1 receptor in nucleus accumbens.
abstract: Propofol, a widely used anesthetic, can cause addictive behaviors in both human and experimental animals. In the present study, we examined the involvement of glucocorticoid receptor (GR) signaling in the molecular process by which propofol may cause addiction. The propofol self-administration model was established by a fixed ratio 1 (FR1) schedule of reinforced dosing over successive 14days in rats. On day 15, the rats were treated with dexamethasone, a GR agonist (10-100μg/kg), or RU486, a GR antagonist (10-100μg/kg) at 1h prior to the last training. The animal behaviors were recorded automatically by the computer. The expression of dopamine D1 receptor in the nucleus accumbens (NAc) was examined by Western blot and the concentrations of plasma corticosterone were measured by enzyme-linked immunosorbent assay (ELISA). To further examine the specificity of GR in the process, mineralocorticoid receptor (MR) antagonist, spironolactone, and dexamethasone plus MR agonist, aldosterone, were also tested. Administration of dexamethasone (100μg/kg) or RU486 (⩾10mg/kg) significantly attenuated the rate of propofol maintained active nose-poke responses and infusions, which were accompanied by reductions in both plasma corticosterone level and the expression of D1 receptor in the NAc. Neither spironolactone alone nor dexamethasone combined with aldosterone affected the propofol-maintaining self-administrative behavior, indicating GR, but not MR, modulates the propofol reward in rats. In addition, neither the food-maintaining sucrose responses under FR1 schedule nor the locomotor activity was affected by any doses of dexamethasone or RU486 tested. These findings provide evidence that GR signaling may play an important role in propofol reward.
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Zhanglei
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Zhichuan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Gaolong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Wenxuan
authors.initials: W
authors.lastname: Lin
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Sicong
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Benfu
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: raygee0828@163.com.
authors.forename: Ren-Shan
authors.initials: RS
authors.lastname: Ge
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: lianqingquanmz@163.com.
authors.forename: Qingquan
authors.initials: Q
authors.lastname: Lian
mesh_terms: D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D003345:Corticosterone / Q000097:blood; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005938:Glucocorticoids / Q000494:pharmacology; D006727:Hormone Antagonists / Q000494:pharmacology; D006993:Hypnotics and Sedatives / Q000008:administration & dosage*; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000378:metabolism; D015742:Propofol / Q000008:administration & dosage*; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018161:Receptors, Mineralocorticoid / Q000819:agonists / Q000378:metabolism; D012646:Self Administration; D019966:Substance-Related Disorders / Q000188:drug therapy / Q000378:metabolism*
chemical_list: D005938:Glucocorticoids; D006727:Hormone Antagonists; D006993:Hypnotics and Sedatives; D000451:Mineralocorticoid Receptor Antagonists; D017447:Receptors, Dopamine D1; D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; D015735:Mifepristone; D000450:Aldosterone; D003907:Dexamethasone; D003345:Corticosterone; D015742:Propofol
keywords: D1 receptor; corticosterone; dexamethasone; propofol; self-administration