title: Synergistic radical scavenging potency of curcumin-in-β-cyclodextrin-in-nanomagnetoliposomes.
abstract: Curcumin is a highly potent nutraceutical associated with various health benefits. However, its hydrophobic nature affects its bioavailability and bioactivity, and limits nutraceutical applications. Drug-in-cyclodextrin-in-liposome has the ability to mask the hydrophobic nature of drug and achieve better encapsulation. Also, encapsulating iron oxide nanoparticles (IONPs) within liposomes endow additional beneficial functionalities of IONPs. In the present study, curcumin-β-cyclodextrin inclusion complex (IC) and IONPs were co-encapsulated within liposomes (curcumin-in-β-cyclodextrin-in-nanomagnetoliposomes) to achieve the synergistic antioxidant potential of curcumin and IONPs. IC of curcumin-β-cyclodextrin was prepared by a simple rapid method and successful inclusion was confirmed by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). Mean diameter of IONPs was found to be 180nm and X-ray diffraction pattern confirmed the formation of hematite nanoparticles. Band gap energy calculated using absorption spectra was 2.25eV, which falls in close proximity with the theoretically calculated values of hematite. Mean diameter of curcumin-in-β-cyclodextrin-in-nanomagnetoliposomes was 67nm and encapsulation efficiency of curcumin was found to be 71%. Further, the co-encapsulated particles possessed significantly low IC50 value (64.7791μg/ml, p<0.01) compared to conventional curcumin liposome and IONPs, indicating its synergistically enhanced radical scavenging property.
authors.affiliation: Centre for Food Nanotechnology, Department of Food Engineering, CSIR-Central Food Technological Research Institute, Mysore, 570 020, Karnataka, India.
authors.forename: W
authors.initials: W
authors.lastname: Aadinath
authors.affiliation: Centre for Food Nanotechnology, Department of Food Engineering, CSIR-Central Food Technological Research Institute, Mysore, 570 020, Karnataka, India.
authors.forename: Anu
authors.initials: A
authors.lastname: Bhushani
authors.affiliation: Centre for Food Nanotechnology, Department of Food Engineering, CSIR-Central Food Technological Research Institute, Mysore, 570 020, Karnataka, India. Electronic address: anandhram@cftri.res.in.
authors.forename: C
authors.initials: C
authors.lastname: Anandharamakrishnan
mesh_terms: D003474:Curcumin / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D008081:Liposomes; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D008081:Liposomes; D058185:Magnetite Nanoparticles; D047392:beta-Cyclodextrins; D003474:Curcumin
keywords: Curcumin; Inclusion complex; Iron oxide nanoparticles; Liposomes; Radical scavenging activity