title: Point-of-Care Detection of β-Lactamase in Milk with a Universal Fluorogenic Probe.
abstract: The illegal addition of β-lactamase (Bla) in milk to disguise β-lactam antibiotics has been a serious issue in the milk industry worldwide. Herein, we report a method for point-of-care detection of Bla based on a probe, Tokyo Green-tethered β-lactam (CDG-1), as a common substrate of various Blas (Bla A, B...) which can enzymatically convert CDG-1 (low fluorescence) to Tokyo Green (high fluorescence). This approach allows rapid screening of a broad spectrum of Blas in real milk samples within 15 min without any pretreatment. Combined with the immuno-magnetic separation, we achieved sensitive and quantitative detection of Bla (10(-5) U/mL), which provides a universal platform for screening and determining Blas in complex samples with high efficiency and accuracy.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , 11 BeiYiTiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , 11 BeiYiTiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Xianyu
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , 11 BeiYiTiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , 11 BeiYiTiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Wenfu
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University , 1201 Welch Road, Stanford, California 94305-5484, United States.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Rao
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , 11 BeiYiTiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D019095:Point-of-Care Systems; D047090:beta-Lactams / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D047090:beta-Lactams