title: Lipid peroxidation in the pathogenesis of neuroborreliosis.
abstract: This study analyzed the onset of lipid peroxidation (LPO) in neuroborreliosis and the effects of ceftriaxone therapy on LPO. Twenty-two patients with early neuroborreliosis and 22 healthy subjects were studied. LPO in the cerebrospinal fluid (CSF), as well as the plasma and urine was estimated by the levels of reactive aldehydes: 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal, malondialdehyde, and 4-oxononenal, F2-isoprostanes and A4/J4-neuroprostanes (NPs). The plasma level of 4-HNE-protein adducts arachidonic acid (AA), docosahexaenoic acid (DHA) and vitamin E was determined. Additionally, enzymatic activities of phospholipase A2 (PLA2), platelet-activating factor acetylhydrolase (PAF-AH) and glutathione peroxidase (GSH-Px) were determined. A decrease of AA, DHA levels and GSH-Px activity in plasma was associated with a significant increase of aldehydes in the CSF, plasma and urine. Similarly, the increase of F2-isoprostanes and NPs in the CSF and plasma was associated with the decreased activity of PLA2 and PAF-AH. Ceftriaxone therapy cured patients and reduced the levels of F2-isoprostanes, NPs and reactive aldehydes. However, the activities of PLA2 and PAF-AH increased. Pathophysiological association of neuroborreliosis with systemic LPO was revealed. Effective antibiotic therapy attenuated LPO. Biomarkers of LPO could be useful to monitor the onset of neuroborreliosis and show the effectiveness of pharmacotherapy.
authors.affiliation: Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Żurawia 14, 15-540 Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Moniuszko-Malinowska
authors.affiliation: Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2d, 15-222 Bialystok, Poland. Electronic address: wojciech.luczaj@umb.edu.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Łuczaj
authors.affiliation: Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2d, 15-222 Bialystok, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Jarocka-Karpowicz
authors.affiliation: Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Żurawia 14, 15-540 Bialystok, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Pancewicz
authors.affiliation: Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Żurawia 14, 15-540 Bialystok, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Zajkowska
authors.affiliation: LabOS, Rudjer Boskovic Institute, Zagreb, Croatia.
authors.forename: Luka
authors.initials: L
authors.lastname: Andrisic
authors.affiliation: LabOS, Rudjer Boskovic Institute, Zagreb, Croatia.
authors.forename: Neven
authors.initials: N
authors.lastname: Zarkovic
authors.affiliation: Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2d, 15-222 Bialystok, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Skrzydlewska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000447:Aldehydes / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D025065:Borrelia burgdorferi / Q000378:metabolism* / Q000472:pathogenicity; D002443:Ceftriaxone / Q000008:administration & dosage; D004281:Docosahexaenoic Acids / Q000378:metabolism; D028441:F2-Isoprostanes / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D005260:Female; D005979:Glutathione Peroxidase / Q000235:genetics; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D020852:Lyme Neuroborreliosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000188:drug therapy* / Q000652:urine; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D054467:Phospholipases A2 / Q000097:blood
chemical_list: D000447:Aldehydes; D028441:F2-Isoprostanes; C063409:4-hydroxyhexenal; D004281:Docosahexaenoic Acids; D008315:Malondialdehyde; D002443:Ceftriaxone; D005979:Glutathione Peroxidase; D054467:Phospholipases A2; C027576:4-hydroxy-2-nonenal
keywords: 4-Hydroxy-2-nonenal; CSF; F(2)-isoprostaneas; Fatty acids; Lipid peroxidation; Neuroborreliosis; Neuroprostanes; Phospholipases; Plasma; Reactive aldehydes; Urine