title: Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses.
abstract: In this study, we evaluated the immune responses of avian metapneumovirus harboring chicken Fc molecule. Stable Vero cells expressing chicken Fc chimera on its surface (Vero-cFc) were established, and we confirmed that aMPV grown in Vero-cFc incorporated host derived chimera Fc into the aMPV virions. Immunization of chicken with aMPV-cFc induced higher level of antibodies and inflammatory cytokines; (Interferon (IFN)-γ and Interleukin (IL)-1β) compared to those of aMPV. The increased levels of antibodies and inflammatory cytokines in chicken immunized with aMPV-cFc were statistically significantly (p<0.05) to that of aMPV and control. The aMPV-cFc group also generated the highest neutralizing antibody response. After challenges, chickens immunized with aMPV-cFc showed much less pathological signs in nasal turbinates and trachea so that we could confirm aMPV-cFc induced higher protection than that of aMPV. The greater ability of aMPV harboring chicken Fc to that of aMPV presented it as a possible vaccine candidate.
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Sarita
authors.initials: S
authors.lastname: Paudel
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Maheswaran
authors.initials: M
authors.lastname: Easwaran
authors.affiliation: Komipharm Co., Ltd., Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Jang
authors.affiliation: Komipharm Co., Ltd., Republic of Korea.
authors.forename: Ho-Kyoung
authors.initials: HK
authors.lastname: Jung
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Komipharm Co., Ltd., Republic of Korea.
authors.forename: Joo-Hun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea. Electronic address: shin0089@cnu.ac.kr.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Shin
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002645:Chickens / Q000276:immunology* / Q000821:virology; D002522:Chlorocebus aethiops; D015870:Gene Expression; D007114:Immunization; D007141:Immunoglobulin Fc Fragments / Q000235:genetics*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000276:immunology; D029121:Metapneumovirus / Q000187:drug effects / Q000254:growth & development / Q000276:immunology; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D010957:Plasmids / Q000737:chemistry / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007141:Immunoglobulin Fc Fragments; D053583:Interleukin-1beta; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma
keywords: Antibody response; Avian metapneumovirus (aMPV); Chicken Fc; Cytokines; Neutralizing antibodies; Vaccines