title: Manipulation of reproductive performance of lactating buffaloes using melatonin and controlled internal drug release device treatment during out-of-breeding season under tropical conditions.
abstract: Twelve lactating Murrah buffalo, divided into control and treatment group of six animals each, were used to study the effect of melatonin and controlled internal drug release (CIDR) device treatment on the resumption of ovarian activity during out-of-breeding season (summer solstice). Treated group implanted with melatonin (18-mg melatonin/50-kg body weight) for 45 days and then animals of both groups received CIDR for 9 days. All animals received intramuscular 500 IU eCG, at day before CIDR removal, and 10-μg GnRH at day after CIDR withdrawal. All animals were subjected to estrus detection daily. Blood samples in conjunction with transrectal ultrasonography were performed once a week to determine serum concentrations of melatonin, progesterone, and antioxidant enzyme activities, as well as to monitor the ovarian activity. Melatonin treatment resulted in an increase (P < 0.01) in the overall mean superoxide dismutase activity. Melatonin and CIDR increased the diameter of CL (P < 0.01) and plasma progesterone concentration (P < 0.05). In addition, melatonin and CIDR exhibited superior ability to maintain presence of CL at Day 21 and Day 30 after artificial insemination and achieved higher percentage of conception rate than control. In conclusion, the CIDR treatment preceded by melatonin improved the reproductive performance in lactating buffaloes during out-of-breeding season under tropical conditions.
authors.affiliation: Agricultural Research Center, Animal Production Research Institute, Giza, Egypt. Electronic address: tamereweda1174@Yahoo.com.
authors.forename: T A
authors.initials: TA
authors.lastname: Ramadan
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Phulia
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Balhara
authors.affiliation: Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Ghuman
authors.affiliation: Division of Animal Physiology and Reproduction, Central Institute for Research on Buffaloes, Hisar, Haryana, India.
authors.forename: I
authors.initials: I
authors.lastname: Singh
mesh_terms: D000282:Administration, Intravaginal; D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology*; D004340:Drug Contamination; D004343:Drug Implants; D004973:Estrus Synchronization; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007774:Lactation; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*; D012621:Seasons; D014329:Tropical Climate
chemical_list: D006063:Chorionic Gonadotropin; D004343:Drug Implants; D011374:Progesterone; D008550:Melatonin
keywords: Antioxidant enzyme activity; CIDR; Lactating buffalo; Melatonin; Ovarian activity