title: miR-486-5p inhibits cell growth of papillary thyroid carcinoma by targeting fibrillin-1.
abstract: Previous studies have demonstrated that miR-486-5p functions as a tumor suppressor or oncogene in various types of cancer. In the present study, we showed that miR-486-5p was significantly down-regulated in papillary thyroid carcinoma (PTC) tissues and cell lines, whereas miR-486-5p down-regulation inhibited PTC cell proliferation and increased apoptosis. Conversely, under-expression of miR-486-5p enhanced PTC cell proliferation and decreased apoptosis. Fibrillin-1 (FBN1) was shown to be a direct target of miR-486-5p and inversely regulated by miR-486-5p. FBN1 silencing led to decreased PTC cell proliferation and enhanced apoptosis in vitro, similar to that mediated by miR-486-5p. Furthermore, miR-486-5p over-expression or FBN1 knock-down inhibited, while up-regulation of FBN1 boosted xenograft tumor formation in vivo. Our data suggest that miR-486-5p induces PTC cell growth inhibition and apoptosis by targeting and suppressing FBN1. Thus, miR-486-5p/FBN1 might provide a promising therapeutic target for PTC treatment.
authors.affiliation: Medical Care Department for Personnel, Urumchi General Hospital of Lanzhou Military Region, Urumchi, Xinjiang 830000, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Endocrinology, Urumchi General Hospital of Lanzhou Military Region, Urumchi, Xinjiang 830000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Pharmacy, Urumchi General Hospital of Lanzhou Military Region, Urumchi, Xinjiang 830000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Urumchi Chinese Medicine Hospital, Urumchi, Xinjiang 830000, China.
authors.forename: Deqiang
authors.initials: D
authors.lastname: Deng
authors.affiliation: Department of Endocrinology, Shanghai Punan Hospital, Shanghai 200125, China.
authors.forename: Lianyong
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, The People's Hospital in Altay Region, Altay, Xinjiang 836500, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Mei
authors.affiliation: Department of Endocrinology, Urumchi General Hospital of Lanzhou Military Region, Urumchi, Xinjiang 830000, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Endocrinology, Urumchi General Hospital of Lanzhou Military Region, Urumchi, Xinjiang 830000, China. Electronic address: jianqing0991@163.com.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Tian
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology*; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D000071838:Fibrillin-1 / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020413:3' Untranslated Regions; D000071838:Fibrillin-1; C560945:MIRN486 microRNA, human; D035683:MicroRNAs
keywords: Apoptosis; Papillary thyroid carcinoma; Proliferation; miR-486-5p