title: Antitumor and Antibacterial Derivatives of Oridonin: A Main Composition of Dong-Ling-Cao.
abstract: Isodon rubescens has been used as a traditional green tea for more than 1000 years and many medicinal functions of I. rubescens are also very useful, such as its well-known antitumor and antibacterial activities. Oridonin, a bioactive ent-kaurane diterpenoid, is the major ingredient of this medicinal tea. Herein, 22 novel oridonin derivatives were designed and synthesized. The antibacterial activity was evaluated for the first time. Compound 12 was the most promising one with MIC of 2.0 μg/mL against B. subtilis, which was nearly 3-fold stronger than positive control chloromycetin. The antiproliferative property was also assayed and compound 19 showed stronger activity than taxol. The apoptosis-inducing ability, cell cycle arrest effect at S phase and influence of mitochondrial membrane potential by 19 in CaEs-17 cancer cells were first disclosed. Based on the above results, the cell apoptosis induced by compound 19 in CaEs-17 cells was most probably involved in the intrinsic apoptotic pathway.
authors.affiliation: Key Laboratory of Structure-Based Drug Design &amp; Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. lidahong0203@163.com.|State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China. lidahong0203@163.com.|State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Processes, National Post-Doctoral Research Workstation, Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, China. lidahong0203@163.com.|State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China. lidahong0203@163.com.
authors.forename: Dahong
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Structure-Based Drug Design &amp; Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. hantong1221@163.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Han
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China. cpuxst@163.com.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Structure-Based Drug Design &amp; Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. tingtingzhou1118@hotmail.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Structure-Based Drug Design &amp; Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. kangtaotian@126.com.
authors.forename: Kangtao
authors.initials: K
authors.lastname: Tian
authors.affiliation: Key Laboratory of Structure-Based Drug Design &amp; Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. huxu105@163.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China. kgcheng2008@gmail.com.
authors.forename: Keguang
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Structure-Based Drug Design &amp; Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. lzl1030@hotmail.com.
authors.forename: Zhanlin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Structure-Based Drug Design &amp; Discovery, Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. huimhua@163.com.
authors.forename: Huiming
authors.initials: H
authors.lastname: Hua
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China. jinyixu@china.com.
authors.forename: Jinyi
authors.initials: J
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D037602:Isodon / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane; D010936:Plant Extracts; C011959:oridonin
keywords: Isodon rubescens; diterpenoid; medicinal chemistry; medicinal tea; oridonin