title: Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography.
abstract: Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown. In this study, affinity chromatography was conducted by coupling periplocoside E-semi-succinic acid ester with epoxy amino hexyl (EAH) sepharose 4B. Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) was performed to analyze the fraction eluted by periplocoside E. Eight binding proteins (luciferin 4-monooxygenase, aminopeptidase N, aminopeptidase N3, nicotinamide adenine dinucleotide health (NADH) dehydrogenase subunit 5, phosphatidylinositol 3-phosphate 3-phosphatase myotubularin, actin, uncharacterized family 31 glucosidase KIAA1161, and 2OG-Fe(2) oxygenase superfamily protein) were obtained and identified through liquid chromatography/quadrupole-time of flight-mass spectrometry (LC/Q-TOF-MS) analysis of the midgut epithelium cells of Mythimna separata larvae. Aminopeptidase N and N3 are potential putative targets of periplocosides. This study establishes the foundation for further research on the mechanism of action and target localization of periplocosides in agricultural pests.
authors.affiliation: Key Laboratory of Botanical Pesticide R &amp; D in Shaanxi Province, Yangling 712100, Shaanxi, China. fengmx2010@126.com.|Institute of Pesticide Science, College of Plant Protection, Northwest A &amp; F University, Yangling 712100, Shaanxi, China. fengmx2010@126.com.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Feng
authors.affiliation: Key Laboratory of Botanical Pesticide R &amp; D in Shaanxi Province, Yangling 712100, Shaanxi, China. hezhenyubobo@163.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: He
authors.affiliation: Institute of Pesticide Science, College of Plant Protection, Northwest A &amp; F University, Yangling 712100, Shaanxi, China. yuanquanerm@163.com.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Pesticide Science, College of Plant Protection, Northwest A &amp; F University, Yangling 712100, Shaanxi, China. xiufangyan@163.com.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Yan
authors.affiliation: Key Laboratory of Botanical Pesticide R &amp; D in Shaanxi Province, Yangling 712100, Shaanxi, China. nwzjw@nwsuaf.edu.cn.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Botanical Pesticide R &amp; D in Shaanxi Province, Yangling 712100, Shaanxi, China. huzhaonong@nwsuaf.edu.cn.|Institute of Pesticide Science, College of Plant Protection, Northwest A &amp; F University, Yangling 712100, Shaanxi, China. huzhaonong@nwsuaf.edu.cn.
authors.forename: Zhaonong
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Key Laboratory of Botanical Pesticide R &amp; D in Shaanxi Province, Yangling 712100, Shaanxi, China. wuwenjun@nwsuaf.edu.cn.|Institute of Pesticide Science, College of Plant Protection, Northwest A &amp; F University, Yangling 712100, Shaanxi, China. wuwenjun@nwsuaf.edu.cn.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004952:Esters; D019476:Insect Proteins / Q000302:isolation & purification*; D007306:Insecticides; D007422:Intestines / Q000737:chemistry; D007814:Larva; D007915:Lepidoptera; D008871:Microvilli; D009844:Oligosaccharides; D011283:Pregnenes
chemical_list: D002352:Carrier Proteins; D004952:Esters; D019476:Insect Proteins; D007306:Insecticides; D009844:Oligosaccharides; D011283:Pregnenes; C509135:periplocoside E
keywords: Mythimna separata; affinity chromatography; binding protein; periplocosides E