title: The Engrailed-1 Gene Stimulates Brown Adipogenesis.
abstract: As a thermogenic organ, brown adipose tissue (BAT) has received a great attention in treating obesity and related diseases. It has been reported that brown adipocyte was derived from engrailed-1 (EN1) positive central dermomyotome. However, functions of EN1 in brown adipogenesis are largely unknown. Here we demonstrated that EN1 overexpression increased while EN1 knockdown decreased lipid accumulation and the expressions of key adipogenic genes including PPARγ2 and C/EBPα and mitochondrial OXPHOS as well as BAT specific marker UCP1. Taken together, our findings clearly indicate that EN1 is a positive regulator of brown adipogenesis.
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Critical Care Medicine and Emergency Room, Luhe Hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Fangxiong
authors.initials: F
authors.lastname: Shi
authors.affiliation: Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wanzhu
authors.initials: W
authors.lastname: Jin