title: Cytocompatible cellulose hydrogels containing trace lignin.
abstract: Sugarcane bagasse was used as a cellulose resource to prepare transparent and flexible cellulose hydrogel films. On the purification process from bagasse to cellulose, the effect of lignin residues in the cellulose was examined for the properties and cytocompatibility of the resultant hydrogel films. The cellulose was dissolved in lithium chloride/N,N-dimethylacetamide solution and converted to hydrogel films by phase inversion. In the purification process, sodium hydroxide (NaOH) treatment time was changed from 1 to 12h. This resulted in cellulose hydrogel films having small amounts of lignin from 1.62 to 0.68%. The remaining lignin greatly affected hydrogel properties. Water content of the hydrogel films was increased from 1153 to 1525% with a decrease of lignin content. Moreover, lower lignin content caused weakening of tensile strength from 0.80 to 0.43N/mm(2) and elongation from 45.2 to 26.5%. Also, similar tendency was observed in viscoelastic behavior of the cellulose hydrogel films. Evidence was shown that the lignin residue was effective for the high strength of the hydrogel films. In addition, scanning probe microscopy in the morphological observation was suggested that the trace lignin in the cellulose hydrogel affected the cellulose fiber aggregation in the hydrogel network. The trace of lignin in the hydrogels also influenced fibroblast cell culture on the hydrogel films. The hydrogel film containing 1.68% lignin showed better fibroblast compatibility as compared to cell culture polystyrene dish used as reference.
authors.affiliation: Department of Materials Science and Technology, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakasone
authors.affiliation: Department of Materials Science and Technology, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan. Electronic address: takaomi@nagaoakut.ac.jp.
authors.forename: Takaomi
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D002482:Cellulose / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D031786:Saccharum / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D002482:Cellulose; D008031:Lignin; C027433:bagasse
keywords: Cellulose; Cytocompatibility; Hydrogel; Lignin; Sugarcane bagasse