title: The effect of the melatonin on cryopreserved mouse testicular cells.
abstract: BACKGROUND
  After improvements in various cancer treatments, life expectancy has been raised, but success in treatment causes loss of fertility in many of the survived young men. Cryopreservation of immature testicular tissues or cells introduced as the only way to preserve fertility. However, freezing has some harmful effects. Melatonin, a pineal gland hormone, has receptors in reproductive systems of different species. It is assumed that melatonin has free radical scavenger properties.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the effects of melatonin on the cryopreserved testicular cells in mouse.
  
  
  METHODS
  Cells from 7- 10 days old NMRI mice testes were isolated using two step enzymatic digestion. The testicular cells were divided into two groups randomly and cryopreserved in two different freezing media with and without the addition of 100 µm melatonin. Finally, apoptosis of the cells was assayed by flow cytometry. Also, lactate dehydrogenase activity test was performed to assess the cytotoxicity.
  
  
  RESULTS
  The results of lactate dehydrogenase showed the nearly cytotoxic effect of melatonin. The results of flow cytometry showed increase in apoptosis in the cryopreserved cells in the media containing melatonin compared to the control group.
  
  
  CONCLUSIONS
  The present study shows that melatonin has an apoptotic effect on cryopreserved mouse testicular cells.
authors.affiliation: Cellular and Molecular research Center, School of Medicine. Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Ghasem
authors.initials: G
authors.lastname: Saki
authors.affiliation: Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mehri
authors.initials: M
authors.lastname: Mirhoseini
authors.affiliation: Infertility and Perinatology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Hemadi
authors.affiliation: Petroleum and Environmental Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khodadadi
authors.affiliation: Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Beygom Talebpour Amiri
keywords: Apoptosis; Melatonin; Mouse; Testicular cells