title: Diversity and effect of Trichoderma spp. associated with green mold disease on Lentinula edodes in China.
abstract: Lentinula edodes, one of the most important edible mushrooms in China, is affected heavily by the infection of green mold that overgrows mushroom mycelia. We collected the diseased samples from main L. edodes cultivation regions in China to characterize the pathogen and to study the effect of Trichoderma spp. on L. edodes species. We identified six Trichoderma species, that is, T. harzianum, T. atroviride, T. viride, T. pleuroticola, T. longibrachiatum, and T. oblongisporum based on the internal transcribed spacer or tef1-α sequences and morphology characteristics. In confrontation cultures on Petri plates or in tubes, and in L. edodes cultures in a medium containing Trichoderma metabolites, L. edodes mycelia were not only distorted and swollen, but also inhibited by Trichoderma isolates. It is not possible that adjusting pH value or temperature is used for controlling L. edodes green disease, because the growth of most of Trichoderma isolates and L. edodes shared similar pH and temperature conditions.
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd, Wuhan, Hubei, 430070, China.|Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China.
authors.forename: Gangzheng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd, Wuhan, Hubei, 430070, China.|Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China.
authors.forename: Xiantao
authors.initials: X
authors.lastname: Cao
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd, Wuhan, Hubei, 430070, China.|Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd, Wuhan, Hubei, 430070, China.|Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China.
authors.forename: Mengpei
authors.initials: M
authors.lastname: Guo
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd, Wuhan, Hubei, 430070, China.|Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China.
authors.forename: Changhao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd, Wuhan, Hubei, 430070, China.|Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China.
authors.forename: Lianlian
authors.initials: L
authors.lastname: Yan
authors.affiliation: Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd, Wuhan, Hubei, 430070, China.|Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China.
authors.forename: Yinbing
authors.initials: Y
authors.lastname: Bian
mesh_terms: D002681:China; D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D058889:Molecular Typing / Q000379:methods; D016533:Mycological Typing Techniques / Q000379:methods; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D020942:Shiitake Mushrooms; D014242:Trichoderma / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic; D020648:Peptide Elongation Factor 1
keywords: Effect of Trichoderma spp. on L. edodes; Lentinula edodes; Trichoderma spp.; green mold; species diversity and distribution