title: NDRG1 Controls Gastric Cancer Migration and Invasion through Regulating MMP-9.
abstract: The purpose of this study is to detect the clinical significance of NDRG1 and its relationship with MMP-9 in gastric cancer metastatic progression. 101 cases of gastric cancer specimens were utilized to identify the protein expression of NDRG1 and MMP-9 by immunohistochemistry, their clinical significance was also analyzed. The suppression by siRNA-NDRG1 was employed to detect the role of NDRG1 in gastric cancer progression and its relationship with MMP-9. NDRG1 expression was correlated inversely with the degree of tumor cell differentiation (p < 0.01), invasion depth (p < 0.05), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), whereas MMP-9 was positive correlated with the degree of tumor cell differentiation (p < 0.01), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), but not correlated with invasion depth (p>0.05). Furthermore, cell proliferation and invasion effect were remarkably enhanced when NDRG1 was silencing, but MMP-9 expression was increased. NDRG1 silencing enhances gastric cancer cells progression through upregulating MMP-9. It suggests that NDRG1 may inhibit the metastasis of gastric cancer via regulating MMP-9.
authors.affiliation: Department of Gastrointestinal Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.|Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.|Department of Radiotherapy, The Second Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Chang
authors.affiliation: Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Radiotherapy, The Second Hospital, Hebei Medical University, Shijiazhuang, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Xue
authors.affiliation: Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Jinguo
authors.initials: J
authors.lastname: Ma
authors.affiliation: Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.affiliation: Department of Gastrointestinal Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Shuanglong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhi
authors.affiliation: Department of Gastrointestinal Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, China. daidq63@163.com.|Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, China. daidq63@163.com.|Department of Gastrointestinal Surgery and Cancer Center, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110032, China. daidq63@163.com.
authors.forename: Dongqiu
authors.initials: D
authors.lastname: Dai
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D000099259:N-myc Downstream-Regulated Gene 1 Protein
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D020780:Matrix Metalloproteinase 9; D000099259:N-myc Downstream-Regulated Gene 1 Protein
keywords: Cell differentiation; Gastric cancer; MMP-9; Metastasis; NDRG1