title: Occurrence and analysis of irp2 virulence gene in isolates of Klebsiella pneumoniae and Enterobacter spp. from microbiota and hospital and community-acquired infections.
abstract: Eighty-five isolates of Klebsiella pneumoniae and Enterobacter spp., originating from hospital- and community-acquired infections and from oropharyngeal and faecal microbiota from patients in Recife-PE, Brazil, were analyzed regarding the presence of irp2 gene. This is a Yersinia typical gene involved in the synthesis of siderophore yersiniabactin. DNA sequencing confirmed the identity of irp2 gene in five K. pneumoniae, five Enterobacter aerogenes and one Enterobacter amnigenus isolates. To our knowledge in the current literature, this is the first report of the irp2 gene in E. amnigenus, a species considered an unusual human pathogen, and in K. pneumoniae and E. aerogenes isolates from the normal microbiota and from community infections, respectively. Additionally, the analyses of nucleotide and amino acid sequences suggest the irp2 genes derived from isolates used in this study are more closely related to that of Yersinia pestis P.CE882 than to that of Yersinia enterocolitica 8081. These data demonstrated that K. pneumoniae and Enterobacter spp. from normal microbiota and from community- and hospital-acquired infections possess virulence factors important for the establishment of extra-intestinal infections.
authors.affiliation: Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, 50.732-970, Brazil. Electronic address: ana.lopes.ufpe@gmail.com.
authors.forename: Ana Catarina
authors.initials: AC
authors.lastname: Souza Lopes
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Juliana Falcão
authors.initials: JF
authors.lastname: Rodrigues
authors.affiliation: Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, 50.732-970, Brazil.
authors.forename: Adriane Borges
authors.initials: AB
authors.lastname: Cabral
authors.affiliation: Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, 50.732-970, Brazil.
authors.forename: Maíra Espíndola
authors.initials: ME
authors.lastname: da Silva
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, FIOCRUZ/MS, Recife, PE, Brazil.
authors.forename: Nilma Cintra
authors.initials: NC
authors.lastname: Leal
authors.affiliation: Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, 50.732-970, Brazil.
authors.forename: Vera Magalhães
authors.initials: VM
authors.lastname: da Silveira
authors.affiliation: Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brazil.
authors.forename: Marcos Antônio
authors.initials: MA
authors.lastname: de Morais Júnior
mesh_terms: D001938:Brazil; D017714:Community-Acquired Infections / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology*; D004754:Enterobacter / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D035942:Iron Regulatory Protein 2 / Q000032:analysis* / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000302:isolation & purification; D064307:Microbiota; D009960:Oropharynx / Q000382:microbiology; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics
chemical_list: D037521:Virulence Factors; D035942:Iron Regulatory Protein 2
keywords: Enterobacter; HPI; Klebsiella pneumoniae; irp2