title: Preventive effect of dietary quercetin on disuse muscle atrophy by targeting mitochondria in denervated mice.
abstract: Quercetin is a major dietary flavonoid in fruits and vegetables. We aimed to clarify the preventive effect of dietary quercetin on disuse muscle atrophy and the underlying mechanisms. We established a mouse denervation model by cutting the sciatic nerve in the right leg (SNX surgery) to lack of mobilization in hind-limb. Preintake of a quercetin-mixed diet for 14days before SNX surgery prevented loss of muscle mass and atrophy of muscle fibers in the gastrocnemius muscle (GM). Phosphorylation of Akt, a key phosphorylation pathway of suppression of protein degradation, was activated in the quercetin-mixed diet group with and without SNX surgery. Intake of a quercetin-mixed diet suppressed the generation of hydrogen peroxide originating from mitochondria and elevated mitochondrial peroxisome proliferator-activated receptor-γ coactivator 1α mRNA expression as well as NADH dehydrogenase 4 expression in the GM with SNX surgery. Quercetin and its conjugated metabolites reduced hydrogen peroxide production in the mitochondrial fraction obtained from atrophied muscle. In C2C12 myotubes, quercetin reached the mitochondrial fraction. These findings suggest that dietary quercetin can prevent disuse muscle atrophy by targeting mitochondria in skeletal muscle tissue through protecting mitochondria from decreased biogenesis and reducing mitochondrial hydrogen peroxide release, which can be related to decreased hydrogen peroxide production and/or improvements on antioxidant capacity of mitochondria.
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramotocho, Tokushima, 770-8503, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Mukai
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramotocho, Tokushima, 770-8503, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Matsui
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramotocho, Tokushima, 770-8503, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fujikura
authors.affiliation: Department of Biochemical Science and Technology Faculty of Agriculture, Kagoshima University, 1-12-24, Korimoto, Kagoshima, 890-0065, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Matsumoto
authors.affiliation: Department of Biochemical Science and Technology Faculty of Agriculture, Kagoshima University, 1-12-24, Korimoto, Kagoshima, 890-0065, Japan.
authors.forename: De-Xing
authors.initials: DX
authors.lastname: Hou
authors.affiliation: Institute for Health Care Science, Suntory Wellness Ltd, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Oksaka, 618-8503, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kanzaki
authors.affiliation: Institute for Health Care Science, Suntory Wellness Ltd, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Oksaka, 618-8503, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shibata
authors.affiliation: Bioorganic Research Institute, Suntory Foundation for Life Sciences.
authors.forename: Manabu
authors.initials: M
authors.lastname: Horikawa
authors.affiliation: Research Institute, Suntory Global Innovation Center, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Oksaka, 618-8503, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Iwasa
authors.affiliation: Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Hirasaka
authors.affiliation: Department of Nutritional Physiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramotocho, Tokushima, 770-8503, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nikawa
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramotocho, Tokushima, 770-8503, Japan. Electronic address: terao@tokushima-u.ac.jp.
authors.forename: Junji
authors.initials: J
authors.lastname: Terao
mesh_terms: D000818:Animals; D003714:Denervation; D004032:Diet; D051379:Mice; D008810:Mice, Inbred C57BL; D008931:Mitochondria, Muscle / Q000187:drug effects*; D009133:Muscular Atrophy / Q000517:prevention & control*; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D011794:Quercetin
keywords: Antioxidant; Disuse muscle atrophy; Mitochondria; Oxidative stress; Polyphenol; Quercetin