title: Modified flotation method, an effective technique for recovering helminth eggs in soil.
abstract: There is no established method for recovering helminth eggs in soil has yet to be established. Here, we introduce a novel method for their recovery based on a centrifugal flotation approach using a sucrose solution with a specific gravity of 1.20, with a coverslip being placed on the sucrose-filled centrifuge tube during centrifugation. The recovery of zoonotic Toxocara eggs from soil was more effective when this method was compared with the traditional flotation method. This method detects not only zoonotic Toxocara eggs, but Ascaris lumbricoides, Trichuris trichiura, and other human parasite eggs also, indicating that it can be used for epidemiological studies both in developed and developing countries.
authors.affiliation: Nishikyo Health Center, Kyoto City, 1-2 Katsura Ushitoracho, Nishikyo-ku, Kyoto 615-8083, Japan. Electronic address: saori-h.6321@hotmail.co.jp.
authors.forename: Saori
authors.initials: S
authors.lastname: Horiuchi
authors.affiliation: Department of Parasitology, Faculty of Nursing, Kobe Women's University, 4-7-2 Minatojima-nakamachi, Chuo-ku, Kobe 650-0046, Japan. Electronic address: s-uga@yg.kobe-wu.ac.jp.
authors.forename: Shoji
authors.initials: S
authors.lastname: Uga
keywords: Flotation method; Helminth egg; Recovery; Soil; Sucrose; Toxocara