title: Influence of nitrogen depletion in the growth of N. oleoabundans on the release of cellular components after beadmilling.
abstract: Cell disruption by bead milling of Neochloris oleoabundans grown under nitrogen repleted (NR) and nitrogen depleted (ND) conditions was evaluated based on the release of biochemicals and biomass to the supernatant. Additionally, energy consumption for cell disruption was calculated per kg of released component. Bead milling of NR cells resulted on average in 34% (w/w) release of biochemicals into the supernatant, with a similar composition as the untreated microalgal biomass. With ND cells, the release was higher and more selective, i.e. 57%, 59%, 68% and 56% (w/w) of respectively biomass, proteins, carbohydrates and lipids whereof 92%, 57% and 46% (w/w) respectively of phospho-, glyco- and neutral lipids.
authors.affiliation: Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium; Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV Wageningen, Netherlands.
authors.forename: Emre
authors.initials: E
authors.lastname: Günerken
authors.affiliation: Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium. Electronic address: els.dhondt@vito.be.
authors.forename: Els
authors.initials: E
authors.lastname: D'Hondt
authors.affiliation: Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV Wageningen, Netherlands.
authors.forename: Michel
authors.initials: M
authors.lastname: Eppink
authors.affiliation: Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium.
authors.forename: Kathy
authors.initials: K
authors.lastname: Elst
authors.affiliation: Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV Wageningen, Netherlands.
authors.forename: Rene
authors.initials: R
authors.lastname: Wijffels
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000460:Chlorophyta / Q000254:growth & development* / Q000378:metabolism; D004734:Energy Metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism
chemical_list: D008055:Lipids; D011506:Proteins; D009584:Nitrogen
keywords: Biochemical release; Cell disruption; Microalgae; Nitrogen depletion