title: Susceptibility of isogeneic ginbuna Carassius auratus langsdorfii Temminck et Schlegel to cyprinid herpesvirus-2 (CyHV-2) as a model species.
abstract: Herpesviral haematopoietic necrosis (HVHN), caused by cyprinid herpesvirus-2 (CyHV-2), has affected the commercial production of the goldfish Carassius auratus and gibelio carp Carassius auratus gibelio. High water temperature treatments are reported to reduce the mortality rate of infected goldfish and elicit immunity in the survivors. To define the mechanism by which this intervention induces resistance, clonal ginbuna Carassius auratus langsdorfii, which is closely related to both species and has been used in fish immunology, may represent a promising model species. In this study, we investigated the susceptibility of clonal ginbuna strains to CyHV-2 and the effect of high water temperature treatment on infected ginbuna and goldfish. Experimental intraperitoneal infection with CyHV-2 at 25 °C caused 100% mortality in ginbuna strains, which was accompanied by histopathological changes typical of HVHN. Both infected ginbuna S3n strain and goldfish, exposed to high temperature for 6 days [shifting from 25 °C (permissive) to 34 °C (non-permissive)], showed reduced mortalities after the 1st inoculation, and subsequent 2nd virus challenge to 0%, indicating induction of immunity. It was concluded that ginbuna showed a similar susceptibility and disease development in CyHV-2 infection compared to goldfish, suggesting that ginbuna can be a useful fish model for the study of CyHV-2 infection and immunity.
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nanjo
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yoshii
authors.affiliation: Saitama Fisheries Research Institute, Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fukuda
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Kato
authors.affiliation: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sano
mesh_terms: D000818:Animals; D002460:Cell Line; D004266:DNA Virus Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D004267:DNA Viruses / Q000502:physiology*; D060467:Disease Resistance; D004198:Disease Susceptibility / Q000276:immunology / Q000401:mortality / Q000662:veterinary / Q000821:virology; D005393:Fish Diseases / Q000276:immunology / Q000401:mortality / Q000821:virology*; D006054:Goldfish; D006358:Hot Temperature / Q000009:adverse effects*; D009336:Necrosis / Q000276:immunology / Q000401:mortality / Q000662:veterinary / Q000821:virology; D014867:Water
chemical_list: D014867:Water
keywords: 
  Carassius auratus
  ; 
  Carassius auratus langsdorfii
  ; cyprinid herpesvirus-2; high water temperature treatment; immunity; susceptibility