title: Particulate Oxidative Burden as a Predictor of Exhaled Nitric Oxide in Children with Asthma.
abstract: BACKGROUND
  Epidemiological studies have provided strong evidence that fine particulate matter (PM2.5; aerodynamic diameter ≤ 2.5 μm) can exacerbate asthmatic symptoms in children. Pro-oxidant components of PM2.5 are capable of directly generating reactive oxygen species. Oxidative burden is used to describe the capacity of PM2.5 to generate reactive oxygen species in the lung.
  
  
  OBJECTIVE
  In this study we investigated the association between airway inflammation in asthmatic children and oxidative burden of PM2.5 personal exposure.
  
  
  METHODS
  Daily PM2.5 personal exposure samples (n = 249) of 62 asthmatic school-aged children in Montreal were collected over 10 consecutive days. The oxidative burden of PM2.5 samples was determined in vitro as the depletion of low-molecular-weight antioxidants (ascorbate and glutathione) from a synthetic model of the fluid lining the respiratory tract. Airway inflammation was measured daily as fractional exhaled nitric oxide (FeNO).
  
  
  RESULTS
  A positive association was identified between FeNO and glutathione-related oxidative burden exposure in the previous 24 hr (6.0% increase per interquartile range change in glutathione). Glutathione-related oxidative burden was further found to be positively associated with FeNO over 1-day lag and 2-day lag periods. Results further demonstrate that corticosteroid use may reduce the FeNO response to elevated glutathione-related oxidative burden exposure (no use, 15.8%; irregular use, 3.8%), whereas mold (22.1%), dust (10.6%), or fur (13.1%) allergies may increase FeNO in children with versus children without these allergies (11.5%). No association was found between PM2.5 mass or ascorbate-related oxidative burden and FeNO levels.
  
  
  CONCLUSIONS
  Exposure to PM2.5 with elevated glutathione-related oxidative burden was associated with increased FeNO.
  
  
  BACKGROUND
  Maikawa CL, Weichenthal S, Wheeler AJ, Dobbin NA, Smargiassi A, Evans G, Liu L, Goldberg MS, Godri Pollitt KJ. 2016. Particulate oxidative burden as a predictor of exhaled nitric oxide in children with asthma. Environ Health Perspect 124:1616-1622; http://dx.doi.org/10.1289/EHP175.
authors.affiliation: Department of Environmental Health Sciences, School of Public Health and Health Sciences, University of Massachusetts, Amherst, Massachusetts, USA.
authors.forename: Caitlin L
authors.initials: CL
authors.lastname: Maikawa
authors.forename: Scott
authors.initials: S
authors.lastname: Weichenthal
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Wheeler
authors.forename: Nina A
authors.initials: NA
authors.lastname: Dobbin
authors.forename: Audrey
authors.initials: A
authors.lastname: Smargiassi
authors.forename: Greg
authors.initials: G
authors.lastname: Evans
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Mark S
authors.initials: MS
authors.lastname: Goldberg
authors.forename: Krystal J Godri
authors.initials: KJ
authors.lastname: Pollitt