title: Light-Driven Reconfiguration of a Xanthophyll Violaxanthin in the Photosynthetic Pigment-Protein Complex LHCII: A Resonance Raman Study.
abstract: Resonance Raman analysis of the photosynthetic complex LHCII, immobilized in a polyacrylamide gel, reveals that one of the protein-bound xanthophylls, assigned as violaxanthin, undergoes light-induced molecular reconfiguration. The phototransformation is selectively observed in a trimeric structure of the complex and is associated with a pronounced twisting and a trans-cis molecular configuration change of the polyene chain of the carotenoid. Among several spectral effects accompanying the reconfiguration there are ones indicating a carotenoid triplet state. Possible physiological importance of the light-induced violaxanthin reconfiguration as a mechanism associated with making the pigment available for enzymatic deepoxidation in the xanthophyll cycle is discussed.
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Grudzinski
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.|Department of Cell Biology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University , ul. Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Janik
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bednarska
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Welc
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.|Department of Metrology and Modelling of Agrophysical Processes, Institute of Agrophysics of Polish Academy of Sciences , Doswiadczalna 4, 20-290 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Zubik
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.|Chair and Department of Synthesis and Chemical Technology of Pharmaceutical Substances, Faculty of Pharmacy, Medical University , Chodzki 4a, 20-093 Lublin, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Sowinski
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Luchowski
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Skłodowska University , 20-033 Lublin, Poland.
authors.forename: Wieslaw I
authors.initials: WI
authors.lastname: Gruszecki
mesh_terms: D007536:Isomerism; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018613:Microscopy, Confocal; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D020836:Protein Structure, Quaternary; D013059:Spectrum Analysis, Raman; D018724:Spinacia oleracea / Q000378:metabolism; D024341:Xanthophylls / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; D024341:Xanthophylls; C005613:violaxanthin