title: Long-Term Lysosomes Tracking with a Water-Soluble Two-Photon Phosphorescent Iridium(III) Complex.
abstract: Lysosomes are the stomachs of the cells that degrade endocytosis and intracellular biomacromolecules and participate in various other cellular processes, such as apoptosis and cell migration. The ability of long-term tracking of lysosomes is very important to understand the details of lysosomal functions and to evaluate drug and gene delivery systems. For studying lysosomes, we designed and synthesized a water-soluble triscyclometalated iridium(III) complex (Ir-lyso) attaching morpholine moieties. The phosphorescent intensity of Ir-lyso is responsive to pH and decreases with an increase in the pH but not quenching in high pH. With excellent two-photon properties, Ir-lyso was used to light up the lysosomes in living cells and 3D tumor spheroids. Moreover, Ir-lyso could label lysosomes more than 4 days, so we developed this complex to act as a long-term probe for tracking lysosomes during cell migration and apoptosis. To the best of our knowledge, this is the first paradigm of metal complexes as the two-photon phosphorescent probe for long-term lysosomes tracking.
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Kangqiang
authors.initials: K
authors.lastname: Qiu
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Huaiyi
authors.initials: H
authors.lastname: Huang
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Bingyang
authors.initials: B
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Yukang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Huang
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Liangnian
authors.initials: L
authors.lastname: Ji
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chao
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D056831:Coordination Complexes; D007495:Iridium / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements; D008247:Lysosomes / Q000378:metabolism*; D017785:Photons; D014867:Water / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D049408:Luminescent Agents; D014867:Water; D007495:Iridium
keywords: 3D tumor spheroid; iridium(III) complex; long-term tracking; lysosomes; two-photon imaging