title: Sample Enrichment for Bioanalytical Assessment of Disinfected Drinking Water: Concentrating the Polar, the Volatiles, and the Unknowns.
abstract: Enrichment methods used in sample preparation for the bioanalytical assessment of disinfected drinking water result in the loss of volatile and hydrophilic disinfection byproducts (DBPs) and hence likely tend to underestimate biological effects. We developed and evaluated methods that are compatible with bioassays, for extracting nonvolatile and volatile DBPs from chlorinated and chloraminated drinking water to minimize the loss of analytes. For nonvolatile DBPs, solid-phase extraction (SPE) with TELOS ENV as solid phase performed superior compared to ten other sorbents. SPE yielded >70% recovery of nonpurgeable adsorbable organic halogens (AOX). For volatile DBPs, cryogenic vacuum distillation performed unsatisfactorily. Purge and cold-trap with crushed ice serving as condensation nuclei achieved recoveries of 50-100% for trihalomethanes and haloacetonitriles and approximately 60-90% for purged AOX from tap water. We compared the purgeable versus the nonpurgeable fraction by combining purge-and-trap extraction with SPE. The purgeable DBP fraction enriched with the purge-and-trap method exerted a lower oxidative stress response in mammalian cells than the nonpurgeable DBPs enriched with SPE after purging, while contributions of both fractions to bacterial cytotoxicity was more variable. 37 quantified DBPs explained almost the entire AOX in the purge-and-trap extracts, but <16% in the SPE extracts demonstrating that the nonpurgeable fraction is dominated by unknown DBPs.
authors.affiliation: National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.|Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Duebendorf, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Stalter
authors.affiliation: National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
authors.forename: Leon I
authors.initials: LI
authors.lastname: Peters
authors.affiliation: National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
authors.forename: Elissa
authors.initials: E
authors.lastname: O'Malley
authors.affiliation: National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
authors.forename: Janet Yat-Man
authors.initials: JY
authors.lastname: Tang
authors.affiliation: Advanced Water Management Centre (AWMC), The University of Queensland , Brisbane, Queensland 4072, Australia.
authors.forename: Marion
authors.initials: M
authors.lastname: Revalor
authors.affiliation: Advanced Water Management Centre (AWMC), The University of Queensland , Brisbane, Queensland 4072, Australia.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Farré
authors.affiliation: National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.
authors.forename: Kalinda
authors.initials: K
authors.lastname: Watson
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Duebendorf, Switzerland.|School of Architecture, Civil and Environmental Engineering (ENAC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , CH-1015 Lausanne, Switzerland.
authors.forename: Urs
authors.initials: U
authors.lastname: von Gunten
authors.affiliation: National Research Centre for Environmental Toxicology (Entox), The University of Queensland , Brisbane, Queensland 4108, Australia.|Department of Cell Toxicology, UFZ - Helmholtz Centre for Environmental Research , 04318 Leipzig, Germany.|Environmental Toxicology, Center for Applied Geosciences, Eberhard Karls University , 72074 Tübingen, Germany.
authors.forename: Beate I
authors.initials: BI
authors.lastname: Escher
mesh_terms: D000818:Animals; D004202:Disinfectants; D004203:Disinfection; D060766:Drinking Water; D006801:Humans; D022882:Trihalomethanes; D014874:Water Pollutants, Chemical; D018508:Water Purification
chemical_list: D004202:Disinfectants; D060766:Drinking Water; D022882:Trihalomethanes; D014874:Water Pollutants, Chemical