title: Ectopic expression of a grape aspartic protease gene, AP13, in Arabidopsis thaliana improves resistance to powdery mildew but increases susceptibility to Botrytis cinerea.
abstract: The grape aspartic protease gene, AP13 was previously reported to be responsive, in Chinese wild Vitis quinquangularis cv. 'Shang-24', to infection by Erysiphe necator, the causal agent of powdery mildew disease, as well as to treatment with salicylic acid in V. labrusca×V. vinifera cv. 'Kyoho'. In the current study, we evaluated the expression levels of AP13 in 'Shang-24' in response to salicylic acid (SA), methyl jasmonate (MeJA) and ethylene (ET) treatments, as well as to infection by the necrotrophic fungus, Botrytis cinerea, and the transcript levels of VqAP13 decreased after B. cinerea infection and MeJA treatment, but increased following ET and SA treatments. Transgenic Arabidopsis thaliana lines over-expressing VqAP13 under the control of a constitutive promoter showed enhanced resistance to powdery mildew and to the bacterium Pseudomonas syringae pv. tomato DC3000, and accumulated more callose than wild type plants, while the resistance of transgenic A. thaliana lines to B. cinerea inoculation was reduced. In addition, the expression profiles of various disease resistance- related genes in the transgenic A. thaliana lines following infection by different pathogens were compared to the equivalent profiles in the wild type plants. The results suggest that VqAP13 action promotes the SA dependent signal transduction pathway, but suppresses the JA signal transduction pathway.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; Guangxi Academy of Agricultures Sciences, Nanning, Guangxi 530007, China. Electronic address: guorongrong3434@163.com.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: tumingstar@163.com.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Tu
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: 18700808875@163.com.
authors.forename: Xianhang
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: yzhjiao@163.com.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: wanran2012@nwsuaf.edu.cn.
authors.forename: Ran
authors.initials: R
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: lizhi@nwsuaf.edu.cn.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: wangyuejin@nwsuaf.edu.cn.
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: wangxiping@nwsuaf.edu.cn.
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000382:microbiology* / Q000502:physiology; D001203:Ascomycota / Q000472:pathogenicity*; D057055:Aspartic Acid Proteases / Q000235:genetics / Q000502:physiology*; D020171:Botrytis / Q000472:pathogenicity*; D060467:Disease Resistance / Q000235:genetics / Q000502:physiology*; D004198:Disease Susceptibility / Q000201:enzymology; D000066630:Ectopic Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D010935:Plant Diseases / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000382:microbiology / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D027843:Vitis / Q000201:enzymology* / Q000502:physiology
chemical_list: D057055:Aspartic Acid Proteases
keywords: Arabidopsis thaliana; Aspartic protease; Disease resistance; Jasmonic acid; Salicylic acid; VqAP13