title: Site-Selective Synthesis of (15)N- and (13)C-Enriched Flavin Mononucleotide Coenzyme Isotopologues.
abstract: Flavin mononucleotide (FMN) is a coenzyme for numerous proteins involved in key cellular and physiological processes. Isotopically labeled flavin is a powerful tool for studying the structure and mechanism of flavoenzyme-catalyzed reactions by a variety of techniques, including NMR, IR, Raman, and mass spectrometry. In this report, we describe the preparation of labeled FMN isotopologues enriched with (15)N and (13)C isotopes at various sites in the pyrazine and pyrimidine rings of the isoalloxazine core of the cofactor from readily available precursors by a five-step chemo-enzymatic synthesis.
authors.affiliation: Department of Chemistry, University of Utah , 315 South 1400 East, Salt Lake City, Utah 84112, United States.
authors.forename: Syam Sundar
authors.initials: SS
authors.lastname: Neti
authors.affiliation: Department of Chemistry, University of Utah , 315 South 1400 East, Salt Lake City, Utah 84112, United States.
authors.forename: C Dale
authors.initials: CD
authors.lastname: Poulter
mesh_terms: D002247:Carbon Isotopes; D003067:Coenzymes / Q000138:chemical synthesis / Q000737:chemistry; D005486:Flavin Mononucleotide / Q000138:chemical synthesis* / Q000737:chemistry; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes; D013059:Spectrum Analysis, Raman
chemical_list: D002247:Carbon Isotopes; D003067:Coenzymes; D009587:Nitrogen Isotopes; D005486:Flavin Mononucleotide