title: Interleukin 10 protects primary melanocyte by activation of Stat-3 and PI3K/Akt/NF-κB signaling pathways.
abstract: Vitiligo is a common melanocytopenic disorder of the skin, with acquired focal depigmentation. Normal human skin relies on melanocytes to provide photoprotection and thermoregulation by producing melanin. Interleukin 10 (IL-10) is a pleiotropic immunoregulatory cytokine drawing more and more researchers' attention. The present study was conducted to investigate the effects of IL-10 on melanocytes and elucidate the underlying mechanisms. We proved that IL-10 play no role in regulating melanogenesis of normal human foreskin-derived epidermal melanocytes (NHEM). IL-10 stimulation activated the JAK/Stat-3 and PI3K/Akt signaling pathways. Moreover, IL-10 treatment increased translocation of p65 NF-κB into the nuclear compartment, and up-regulated expression of the pro-survival proteins Bcl-2 and Bcl-xL. IL-10 restored anti-apoptotic proteins expression and suppressed cytochrome c release in H2O2-induced apoptosis. In conclusion, IL-10 may provide pro-survival cues to melanocytes and be applied in the treatment of vitiligo and other depigmenting disorders.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Ling
authors.affiliation: Laboratory Animal Center, Xiamen University, Xiamen, Fujian 361102, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Urological Surgical, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Bainian
authors.initials: B
authors.lastname: Feng
authors.affiliation: Department of Clinical Laboratory Science, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, PR China. Electronic address: pffzjtg2015@163.com.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Ping
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D008544:Melanocytes / Q000378:metabolism* / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism*; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: C000595707:BCL2 protein, human; C494811:BCL2L1 protein, human; C508609:IL10 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; C498143:RELA protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D051996:Transcription Factor RelA; D051020:bcl-X Protein; D016753:Interleukin-10; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: IL-10; Melanogenesis; PI3K/Akt/NF-κB signaling pathways; Protective effect