title: Hydroxycamptothecin shows antitumor efficacy on HeLa cells via autophagy activation mediated apoptosis in cervical cancer.
abstract: OBJECTIVE
  To investigate the effect and the mechanism of anti-tumor agent hydroxycamptothecin (HCPT) on HeLa cells in cervical cancer.
  
  
  METHODS
  Autophagy and apoptosis were detected by western blotting and the transfection of GFP-LC3 shRNA as well as Hoechst staining.
  
  
  RESULTS
  The authors found that the expression of the regulators of Beclin-1, p62, and microtubule-associated protein 1 light chain 3 (LC3) upregulated and then triggered the occurrence of cell autophagy. On the other hand, HCPT could induce to the formation of autophagy and resulted in cell apoptosis after autophagy.
  
  
  CONCLUSIONS
  HCPT can alter cell autophagy and then trigger cell apoptosis to achieve antitumor effects.
authors.forename: Y X
authors.initials: YX
authors.lastname: Cheng
authors.forename: Q F
authors.initials: QF
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Pan
authors.forename: J L
authors.initials: JL
authors.lastname: Huang
authors.forename: B L
authors.initials: BL
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Hu
authors.forename: M Q
authors.initials: MQ
authors.lastname: Li
authors.forename: Ch
authors.initials: Ch
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000187:drug effects / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1; D015153:Blotting, Western; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000187:drug effects / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism; D002583:Uterine Cervical Neoplasms; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; C494826:BAX protein, human; C000595707:BCL2 protein, human; C491997:BECN1 protein, human; D000071186:Beclin-1; C477012:MAP1LC3A protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; C118585:P62 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D016601:RNA-Binding Proteins; D051028:bcl-2-Associated X Protein; C527042:hydroxycamptothecinum; C505407:CASP3 protein, human; D053148:Caspase 3; D002166:Camptothecin