title: Antiangiogenic Effect of Bevacizumab: Application of Arterial Spin-Labeling Perfusion MR Imaging in a Rat Glioblastoma Model.
abstract: The usefulness of arterial spin-labeling for the evaluation of the effect of the antiangiogenic therapy has not been elucidated. Our aim was to evaluate the antiangiogenic effect of bevacizumab in a rat glioblastoma model based on arterial spin-labeling perfusion MR imaging.
  
  
  
  DSC and arterial spin-labeling perfusion MR imaging were performed by using a 9.4T MR imaging scanner in nude rats with glioblastoma. Rats were randomly assigned to the following 3 groups: control, 3-day treatment, and 10-day treatment after bevacizumab injection. One-way analysis of variance with a post hoc test was used to compare perfusion parameters (eg, normalized CBV and normalized CBF from DSC MR imaging and normalized CBF based on arterial spin-labeling) with microvessel area on histology. The Pearson correlations between perfusion parameters and microvessel area were also determined.
  
  
  
  All of the normalized CBV from DSC, normalized CBF from DSC, normalized CBF from arterial spin-labeling, and microvessel area values showed significant decrease after treatment (P < .001, P < .001, P = .005, and P < .001, respectively). In addition, normalized CBV and normalized CBF from DSC and normalized CBF from arterial spin-labeling strongly correlated with microvessel area (correlation coefficient, r = 0.911, 0.869, and 0.860, respectively; P < .001 for all).
  
  
  
  Normalized CBF based on arterial spin-labeling and normalized CBV and normalized CBF based on DSC have the potential for evaluating the effect of antiangiogenic therapy on glioblastomas treated with bevacizumab, with a strong correlation with microvessel area.
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Departments of Radiology (T.J.Y., H.R.C., S.H.C., H.K., J.-h.K., C.-H.S., M.H.H.).
authors.forename: T J
authors.identifier: 0000-0001-8441-4574
authors.initials: TJ
authors.lastname: Yun
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Departments of Radiology (T.J.Y., H.R.C., S.H.C., H.K., J.-h.K., C.-H.S., M.H.H.).
authors.forename: H R
authors.identifier: 0000-0003-2107-5813
authors.initials: HR
authors.lastname: Cho
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Departments of Radiology (T.J.Y., H.R.C., S.H.C., H.K., J.-h.K., C.-H.S., M.H.H.) choiseunghong@gmail.com.
authors.forename: S H
authors.identifier: 0000-0002-0412-2270
authors.initials: SH
authors.lastname: Choi
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Departments of Radiology (T.J.Y., H.R.C., S.H.C., H.K., J.-h.K., C.-H.S., M.H.H.).
authors.forename: H
authors.identifier: 0000-0003-2693-9983
authors.initials: H
authors.lastname: Kim
authors.affiliation: Pathology (J.-K.W.).
authors.forename: J-K
authors.identifier: 0000-0003-1459-8093
authors.initials: JK
authors.lastname: Won
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Department of Radiology (S.-W.P.), Seoul National University Boramae Medical Center, Seoul, Republic of Korea.
authors.forename: S-W
authors.identifier: 0000-0002-5063-2685
authors.initials: SW
authors.lastname: Park
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Departments of Radiology (T.J.Y., H.R.C., S.H.C., H.K., J.-h.K., C.-H.S., M.H.H.).
authors.forename: J-H
authors.identifier: 0000-0002-6349-6950
authors.initials: JH
authors.lastname: Kim
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Departments of Radiology (T.J.Y., H.R.C., S.H.C., H.K., J.-h.K., C.-H.S., M.H.H.).
authors.forename: C-H
authors.identifier: 0000-0003-0039-5746
authors.initials: CH
authors.lastname: Sohn
authors.affiliation: From the Department of Radiology (T.J.Y., H.R.C., S.H.C., H.K., S.-W.P., J.-h.K., C.-H.S., M.H.H.), Seoul National University College of Medicine, Republic of Korea Departments of Radiology (T.J.Y., H.R.C., S.H.C., H.K., J.-h.K., C.-H.S., M.H.H.) Neurosurgery (M.H.H.), Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: M H
authors.identifier: 0000-0001-5476-3482
authors.initials: MH
authors.lastname: Han
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000068258:Bevacizumab / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging*; D000071937:Cerebral Blood Volume / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000187:drug effects; D005909:Glioblastoma / Q000098:blood supply* / Q000000981:diagnostic imaging*; D008297:Male; D010477:Perfusion; D011897:Random Allocation; D051381:Rats; D013113:Spin Labels
chemical_list: D020533:Angiogenesis Inhibitors; D013113:Spin Labels; D000068258:Bevacizumab