title: Molecular phylogeny and biotechnological potential of bacterial endophytes associated with Malpighia emarginata.
abstract: Acerola (Malpighia emarginata) is a shrub native to tropical and subtropical climates, which has great commercial interest due to the high vitamin C content of its fruit. However, there are no reports of the endophytic community of this plant species. The aim of this study was to verify the genetic diversity of the leaf endophytic bacterial community of two varieties (Olivier & Waldy Cati 30) of acerola, and to evaluate their biotechnological ability by assessing their in vitro control of pathogenic fungi and the enzymatic production of cellulase, xylanase, amylase, pectinase, protease, lipase, esterase, and chitinase. In total, 157 endophytic bacteria were isolated from the leaves of two varieties of the plant at 28° and 37°C. Phylogenetic analysis confirmed the molecular identification of 58 bacteria, 39.65% of which were identified at the species level. For the first time, the genus Aureimonas was highlighted as an endophytic bacterium. Furthermore, 12.82% of the isolates inhibited the growth of all phytopathogens evaluated and at least one of the above-mentioned enzymes was produced by 64.70% of the endophytes, demonstrating that M. emarginata isolates have potential use in biotechnological studies.
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: V
authors.initials: V
authors.lastname: Specian
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: A T
authors.initials: AT
authors.lastname: Costa
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: A C
authors.initials: AC
authors.lastname: Felber
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: J C
authors.initials: JC
authors.lastname: Polonio
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: J L
authors.initials: JL
authors.lastname: Azevedo
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: J A
authors.initials: JA
authors.lastname: Pamphile
mesh_terms: D000681:Amylases / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D002480:Cellulase / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D060026:Endophytes / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D007218:Industrial Microbiology; D008049:Lipase / Q000378:metabolism; D029581:Malpighiaceae / Q000382:microbiology*; D064307:Microbiota; D010447:Peptide Hydrolases / Q000378:metabolism; D010802:Phylogeny; D011096:Polygalacturonase / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism
chemical_list: D008049:Lipase; D000681:Amylases; D014995:Xylosidases; D002688:Chitinases; D011096:Polygalacturonase; D002480:Cellulase; D010447:Peptide Hydrolases