title: Inhibition of Human Glioma Cell Proliferation Caused by Knockdown of Utrophin Using a Lentivirus-Mediated System.
abstract: BACKGROUND
  Glioma is the most devastating brain tumor worldwide. Previous studies showed that UTRN (utrophin) was related to cancers, but its role in glioma cells remains uncovered.
  
  
  METHODS
  RNAi was used to knockdown UTRN in U251 cells using lentivirus system. The knockdown efficiency was validated by real-time quantitative PCR. Cell proliferation, cell cycle, and apoptosis progression were determined by MTT, colony formation analysis, and flow cytometry analysis. Furthermore, some apoptotic markers were examined by Western blot assay.
  
  
  RESULTS
  Most cells were infected. Cell proliferation and colony formation ability were suppressed in U251 cells lacking UTRN. Moreover, there was an obvious increase in cell percentage in the G2/M phases and a significant apoptosis in U251 cells after UTRN silencing. Further investigation demonstrated that UTRN knockdown activated caspase and PARP pathways.
  
  
  CONCLUSIONS
  Knockdown of UTRN expression by shRNA evidently inhibited cell proliferation and promoted cell apoptosis in glioma cells.
authors.affiliation: 1 Department of Neurosurgery, The First Affiliated Hospital of Xiamen University , Medical College of Xiamen University, Xiamen, China .
authors.forename: Shang-Hang
authors.initials: SH
authors.lastname: Shen
authors.affiliation: 1 Department of Neurosurgery, The First Affiliated Hospital of Xiamen University , Medical College of Xiamen University, Xiamen, China .
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.affiliation: 2 Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University , Hefei, China .
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: 1 Department of Neurosurgery, The First Affiliated Hospital of Xiamen University , Medical College of Xiamen University, Xiamen, China .
authors.forename: Xi-Yao
authors.initials: XY
authors.lastname: Liu
authors.affiliation: 1 Department of Neurosurgery, The First Affiliated Hospital of Xiamen University , Medical College of Xiamen University, Xiamen, China .
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Tan
authors.affiliation: 1 Department of Neurosurgery, The First Affiliated Hospital of Xiamen University , Medical College of Xiamen University, Xiamen, China .
authors.forename: Zhan-Xiang
authors.initials: ZX
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy / Q000821:virology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D055785:Gene Knockdown Techniques / Q000379:methods; D005910:Glioma / Q000235:genetics* / Q000473:pathology / Q000628:therapy / Q000821:virology; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection; D049411:Utrophin / Q000172:deficiency / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; C483599:UTRN protein, human; D049411:Utrophin
keywords: Glioma; RNA interference; Utrophin; cell apoptosis