title: Integration of Lupinus angustifolius L. (narrow-leafed lupin) genome maps and comparative mapping within legumes.
abstract: Narrow-leafed lupin (Lupinus angustifolius L.) has recently been considered a reference genome for the Lupinus genus. In the present work, genetic and cytogenetic maps of L. angustifolius were supplemented with 30 new molecular markers representing lupin genome regions, harboring genes involved in nitrogen fixation during the symbiotic interaction of legumes and soil bacteria (Rhizobiaceae). Our studies resulted in the precise localization of bacterial artificial chromosomes (BACs) carrying sequence variants for early nodulin 40, nodulin 26, nodulin 45, aspartate aminotransferase P2, asparagine synthetase, cytosolic glutamine synthetase, and phosphoenolpyruvate carboxylase. Together with previously mapped chromosomes, the integrated L. angustifolius map encompasses 73 chromosome markers, including 5S ribosomal DNA (rDNA) and 45S rDNA, and anchors 20 L. angustifolius linkage groups to corresponding chromosomes. Chromosomal identification using BAC fluorescence in situ hybridization identified two BAC clones as narrow-leafed lupin centromere-specific markers, which served as templates for preliminary studies of centromere composition within the genus. Bioinformatic analysis of these two BACs revealed that centromeric/pericentromeric regions of narrow-leafed lupin chromosomes consisted of simple sequence repeats ordered into tandem repeats containing the trinucleotide and pentanucleotide simple sequence repeats AGG and GATAC, structured into long arrays. Moreover, cross-genus microsynteny analysis revealed syntenic patterns of 31 single-locus BAC clones among several legume species. The gene and chromosome level findings provide evidence of ancient duplication events that must have occurred very early in the divergence of papilionoid lineages. This work provides a strong foundation for future comparative mapping among legumes and may facilitate understanding of mechanisms involved in shaping legume chromosomes.
authors.affiliation: Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, Poznań, 60-479, Poland. kwyr@igr.poznan.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wyrwa
authors.affiliation: Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, Poznań, 60-479, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Książkiewicz
authors.affiliation: Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, Poznań, 60-479, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Szczepaniak
authors.affiliation: Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, Poznań, 60-479, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Susek
authors.affiliation: Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Z. Noskowskiego 12/14, Poznań, 61-704, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Podkowiński
authors.affiliation: Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, Poznań, 60-479, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Naganowska
mesh_terms: D001219:Aspartate Aminotransferases / Q000235:genetics; D001217:Aspartate-Ammonia Ligase / Q000235:genetics; D002503:Centromere / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D031297:Lupinus / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D009586:Nitrogen Fixation / Q000235:genetics; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012230:Rhizobiaceae / Q000235:genetics; D026801:Synteny / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D005819:Genetic Markers; D008565:Membrane Proteins; D010940:Plant Proteins; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; C088179:RNA, ribosomal, 45S; C045625:nodulin; D001219:Aspartate Aminotransferases; D010730:Phosphoenolpyruvate Carboxylase; D001217:Aspartate-Ammonia Ligase; D005974:Glutamate-Ammonia Ligase
keywords: Lupinus angustifolius; centromeres; chromosome markers; integrated map; rDNA; synteny