title: Novel nanocarrier for oral Hepatitis B vaccine.
abstract: Oral vaccination is a safe, cost effective and non-invasive method suitable for mass immunization. We fabricated nanoparticle (NP) with 14kd polycaprolactone (PCL) entrapping hepatitis B surface antigen (HBsAg) stabilized with Pluronics® F127 and used it as oral delivery vehicle. We evaluated its efficacy for specific antibody production and compared with parenteral routes of immunization in mice. We found a superior antibody response with a higher titer of anti-HBsAg antibody till 2 months following single oral administration compared to other routes of immunization and conventional alum-based HBsAg vaccine. The NPs with the antigen were found in the macrophages in small intestinal villi, peripheral lymph nodes and other reticulo-endothelial organs 2 months after oral administration. This study suggests the efficacy of the current nanocarrier system for efficient antigen presentation disseminated in peripheral lymphoid tissues following oral administration with a prolonged antibody response, which can minimize the requirement of booster dose.
authors.affiliation: Department of pathology, All India Institute of medical sciences,Ansari nagar, New Delhi 110029, India. Electronic address: dindaaiims@gmail.com.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Dinda
authors.affiliation: Department of pathology, All India Institute of medical sciences,Ansari nagar, New Delhi 110029, India.
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Bhat
authors.affiliation: Department of pathology, All India Institute of medical sciences,Ansari nagar, New Delhi 110029, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Department of pathology, All India Institute of medical sciences,Ansari nagar, New Delhi 110029, India.
authors.forename: Sarat K
authors.initials: SK
authors.lastname: Kottarath
authors.affiliation: Department of pathology, All India Institute of medical sciences,Ansari nagar, New Delhi 110029, India.
authors.forename: Chandravilas K
authors.initials: CK
authors.lastname: Prashant
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D001672:Biocompatible Materials; D004337:Drug Carriers / Q000008:administration & dosage; D005260:Female; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000008:administration & dosage*; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D008264:Macrophages / Q000276:immunology; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage*; D020442:Poloxamer; D014611:Vaccination / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D020442:Poloxamer
keywords: Hepatitis B; Nanoparticles; Oral Vaccine; Pluronic F127; Polycaprolactone