title: Rapid and facile ratiometric detection of an anthrax biomarker by regulating energy transfer process in bio-metal-organic framework.
abstract: A ratiometric fluorescent sensor based on luminescent bio-metal-organic framework was prepared by exchanging both Tb(3+) and Eu(3+) cations into anionic bio-MOF-1. Due to a highly efficient energy transfer from Tb(3+) to Eu(3+) (>89%), emission color of Tb/Eu@bio-MOF-1 was orange-red even though Tb(3+) was the dominant content in this Tb/Eu co-doping material. More interestingly, this energy transfer process could be modulated by dipicolinic acid (DPA), an unique biomarker for bacillus spores. With DPA addition, corresponding DPA-to-Tb(3+) energy transfer was gradually enhanced while the energy transfer from Tb(3+) to Eu(3+) was significantly weakened. By regulating the energy transfer process in Tb/Eu@bio-MOF-1, visual colorimetric sensing of DPA in porous MOF was realized for the first time. Detection limit of Tb/Eu@bio-MOF-1 for DPA was 34nM, which was much lower than an infectious dosage of Bacillus anthracis spores (60μM) for human being. Besides, Tb/Eu@bio-MOF-1 showed a remarkable selectivity over other aromatic ligands and amino acids. More importantly, this porous ratiometric sensor worked equally well in human serum. These particularly attractive features of Tb/Eu@bio-MOF-1 made the direct, rapid and naked-eye detection of DPA for practical application possible.
authors.affiliation: State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Yihe
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China. Electronic address: libinteacher@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China. Electronic address: mahp@ciomp.ac.cn.
authors.forename: Heping
authors.initials: H
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, PR China; State Key Labortory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Youxuan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000881:Anthrax / Q000097:blood* / Q000175:diagnosis; D001408:Bacillus anthracis / Q000302:isolation & purification*; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods; D003124:Colorimetry / Q000379:methods; D004735:Energy Transfer; D005063:Europium / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry*; D010848:Picolinic Acids / Q000032:analysis / Q000097:blood*; D016062:Porosity; D013725:Terbium / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D049408:Luminescent Agents; D009942:Organometallic Compounds; D010848:Picolinic Acids; D013725:Terbium; D005063:Europium; C004860:dipicolinic acid
keywords: Colorimetric sensor; Dipicolinate acid; Energy transfer; Lanthanide; Metal-organic frameworks