title: Arsenic processing of yeast isolates IIB-As1 & IIB-As2 and production of glutathione under stress conditions.
abstract: Four arsenic resistant yeast were isolated from the industrial wastewater. Two strains IIB-As1 and IIB-As2 identified as Candida tropicalis and Saccharomyces cerevisiae, respectively. IIB-As1 and IIB-As2 showed maximum arsenic resistance. IIB-As1 showed maximum growth at 35 °C whereas it was 30 °C for IIB-As2. The yeast isolate showed typical growth curves, but arsenic extended the lag phase. Glutathione plays an important role in metal tolerance. In the present study, As increased the level glutathione and non-protein thiols in yeast isolates. Removal of As from supernatant was analyzed using the atomic absorption spectrophotometer. They removed arsenic from the medium after 72 h of incubation. Both yeast strains efficiently removed arsenic from the industrial effluent when used individually or in consortia.
authors.affiliation: Institute of Industrial Biotechnology, GC University, Lahore, Pakistan. bushramuneer11@yahoo.co.uk.
authors.forename: Bushra
authors.initials: B
authors.lastname: Muneer
authors.affiliation: Institute of Industrial Biotechnology, GC University, Lahore, Pakistan.
authors.forename: Tayyaba
authors.initials: T
authors.lastname: Lali
authors.affiliation: Department of Zoology, University of the Punjab, Quaid-I-Azam Campus, Lahore, Pakistan.
authors.forename: Muhammad J
authors.initials: MJ
authors.lastname: Iqbal
authors.affiliation: Department of Zoology, University of the Punjab, Quaid-I-Azam Campus, Lahore, Pakistan.
authors.forename: Farah R
authors.initials: FR
authors.lastname: Shakoori
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-I-Azam Campus, Lahore, Pakistan.
authors.forename: Abdul R
authors.initials: AR
authors.lastname: Shakoori
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D041022:Candida tropicalis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004361:Drug Tolerance; D004785:Environmental Pollutants / Q000378:metabolism*; D004787:Environmental Pollution; D005978:Glutathione / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D004785:Environmental Pollutants; D012337:RNA, Ribosomal, 18S; D005978:Glutathione; D001151:Arsenic
keywords: Arsenic processing; Bioremediation; Candida tropicalis; Glutathione; Growth optimization; Saccharomyces cerevisiae