title: Effect of treatment with simvastatin on bone microarchitecture of the femoral head in an osteoporosis animal model.
abstract: The objective of this study was to evaluate the microarchitecture and trabecular bone strength at the distal region of the femur, and its biomechanical properties with simvastatin administration with two different doses in ovariectomized (OVX) rats. Ninety rats were divided into six groups to evaluate treatment with the simvastatin drug (n = 15): SH (Sham surgery), SH-5 (5 mg simvastatin), SH-20 (20 mg simvastatin), OVX, OVX-5, and OVX-20. Euthanasia was performed at three different times, five animals per period: 7, 14, and 28 days. The effectiveness of the treatments was evaluated by mechanical testing and histomorphometric analysis of the femurs. The results of analysis by the linear model of mixed effects showed 20 mg of simvastatin results in increased trabecular bone after 14 days (P = 0.039) of ingestion in ovariectomized animals. However, ingestion of 5 mg of simvastatin is able to sensitize the trabecular bone only at 28 days (P = 0.005) of ingestion. In the mechanical tests stiffness improves within 28 days (P = 0.003). Regarding maximum strength, no statistical differences were observed. According to these results, it can be concluded that for a decrease in oral intake, longer treatment times are required. Microsc. Res. Tech. 79:684-690, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Morphology, Physiology, and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. Café S/N, Monte Alegre 14040-904, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Lucas Oliveira
authors.initials: LO
authors.lastname: Monteiro
authors.affiliation: Bioengineering Laboratory, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Macedo
authors.affiliation: Bioengineering Laboratory, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Roberta Carminati
authors.initials: RC
authors.lastname: Shimano
authors.affiliation: Bioengineering Laboratory, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: Shimano
authors.affiliation: Bioengineering Laboratory, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Gabriela Rezende
authors.initials: GR
authors.lastname: Yanagihara
authors.affiliation: Department of Morphology, Physiology, and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. Café S/N, Monte Alegre 14040-904, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Junia
authors.initials: J
authors.lastname: Ramos
authors.affiliation: Department of Morphology, Physiology, and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. Café S/N, Monte Alegre 14040-904, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Marina Ribeiro
authors.initials: MR
authors.lastname: Paulini
authors.affiliation: Bioengineering Laboratory, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Fellipe Augusto
authors.initials: FA
authors.lastname: Tocchini de Figueiredo
authors.affiliation: Bioengineering Laboratory, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Miliane Gonçalves
authors.initials: MG
authors.lastname: Gonzaga
authors.affiliation: Department of Morphology, Physiology, and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. Café S/N, Monte Alegre 14040-904, Ribeirão Preto, São Paulo, Brazil.|Bioengineering Laboratory, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900, Ribeirão Preto, São Paulo, Brazil.
authors.forename: João Paulo Mardegan
authors.initials: JP
authors.lastname: Issa
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005270:Femur Head / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure*; D010024:Osteoporosis / Q000473:pathology*; D010052:Ovariectomy; D010784:Photomicrography; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000494:pharmacology*
chemical_list: D019821:Simvastatin
keywords: metabolic bone disease; ovariectomy and statins