title: Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood.
abstract: Enzyme-linked immunosorbent assay (ELISA) is a promising method to detect small amount of proteins in biological samples. The devices providing a platform for reduced sample volume and assay time as well as full automation are required for potential use in point-of-care-diagnostics. Recently, we have demonstrated ultrasensitive detection of serum proteins, C-reactive protein (CRP) and cardiac troponin I (cTnI), utilizing a lab-on-a-disc composed of TiO2 nanofibrous (NF) mats. It showed a large dynamic range with femto molar (fM) detection sensitivity, from a small volume of whole blood in 30 min. The device consists of several components for blood separation, metering, mixing, and washing that are automated for improved sensitivity from low sample volumes. Here, in the video demonstration, we show the experimental protocols and know-how for the fabrication of NFs as well as the disc, their integration and the operation in the following order: processes for preparing TiO2 NF mat; transfer-printing of TiO2 NF mat onto the disc; surface modification for immune-reactions, disc assembly and operation; on-disc detection and representative results for immunoassay. Use of this device enables multiplexed analysis with minimal consumption of samples and reagents. Given the advantages, the device should find use in a wide variety of applications, and prove beneficial in facilitating the analysis of low abundant proteins.
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea.
authors.forename: Yang-Seok
authors.initials: YS
authors.lastname: Park
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea.
authors.forename: Vijaya
authors.initials: V
authors.lastname: Sunkara
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea; Current Affiliation is Agency for Defense Development (ADD), Daejeon, Republic of Korea.
authors.forename: Won Seok
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea; Current Affiliation is KOGAS (Korea Gas Corporation) Research Institute, Daegu, Republic of Korea.
authors.forename: Ja-Ryoung
authors.initials: JR
authors.lastname: Han
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea; Center for Soft and Living Matter, Institute for Basic Science (IBS), UNIST-gil 50, Ulsan, Republic of Korea; ykcho@unist.ac.kr.
authors.forename: Yoon-Kyoung
authors.initials: YK
authors.lastname: Cho
mesh_terms: D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D019210:Troponin I / Q000097:blood / Q000302:isolation & purification*
chemical_list: D015415:Biomarkers; D019210:Troponin I; D002097:C-Reactive Protein