title: Identification of a distinct subpopulation of fibroblasts from murine dermis: CD73(-) CD105(+) as potential marker of dermal fibroblasts subset with multipotency.
abstract: Skin dermis includes various types of multipotent stromal cells (MSCs) and a subpopulation of dermal fibroblasts that exhibit the ability to differentiate. However, characterization of this dermal fibroblast subtype remains less understood. In this study, we isolated dermal cells from the skin of newborn C57/B6 mice and investigated their characteristics. Isolated murine dermal cells exhibited a fibroblast phenotype as judged by accepted criteria including a lack of MSC-related antigens and the differentiation potential of MSCs, and the positive expression of fibroblast markers. A comparative analysis demonstrated that CD73(-) CD105(+) but not CD73(-) CD105(-) dermal fibroblasts exhibited some of the functional properties of MSCs. Furthermore, the multipotent phenotype of CD73(-) CD105(+) cells was diminished by treatment of CD105 siRNA and shRNA, indicating that CD105 expression was critical for the retention of differentiation potential of those cells. Overall, these results suggest that CD73(-) CD105(+) cells are a distinct subset of dermal fibroblasts with multipotency and that their surface antigens could help to classify this subpopulation. These cells may contribute to the regeneration of damaged tissue.
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Lee
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Sehwan
authors.initials: S
authors.lastname: Shim
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Hye-Yun
authors.initials: HY
authors.lastname: Shin
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Won-Suk
authors.initials: WS
authors.lastname: Jang
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Sun-Joo
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Young-Woo
authors.initials: YW
authors.lastname: Jin
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Seung-Sook
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Laboratory of Radiation Exposure & Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Science, 75 Nowon-ro, Nowon-gu, Seoul, Republic of Korea.
authors.forename: Sunhoo
authors.initials: S
authors.lastname: Park
mesh_terms: D015720:5'-Nucleotidase / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D000071063:Endoglin / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000145:classification / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D015415:Biomarkers; D000071063:Endoglin; C502004:Eng protein, mouse; D015720:5'-Nucleotidase
keywords: dermis; fibroblasts; multipotent stromal cells; skin