title: Expanding an Efficient, Electrically Driven and CNT-Tagged P450 System into the Third Dimension: A Nanowired CNT-Containing and Enzyme-Stabilising 3 D Sol-Gel Electrode.
abstract: Although electrochemically catalysed P450 reactions have been described, their efficiency and applicability remained limited. This is mostly due to low enzyme activity, laborious protein immobilisation and the small electrode surface. We established a novel protein immobilisation method for a determined orientation and electrical wiring of the enzyme without post-expression modification. By genetic introduction of an anchor-peptide our method is applicable for screening medium to large mutant libraries and detection by an electrode system. The system was expanded by using wired carbon nanotubes within a sol-gel matrix to create a three dimensional electrode.
authors.affiliation: NMI Natural and Medical Sciences Institute, University of Tübingen, Markwiesenstrasse 55, 72770, Reutlingen, Germany.
authors.forename: Enrique C
authors.initials: EC
authors.lastname: Llaudet
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany.
authors.forename: Dominique
authors.initials: D
authors.lastname: Darimont
authors.affiliation: NMI Natural and Medical Sciences Institute, University of Tübingen, Markwiesenstrasse 55, 72770, Reutlingen, Germany.
authors.forename: Ramona
authors.initials: R
authors.lastname: Samba
authors.affiliation: NMI Natural and Medical Sciences Institute, University of Tübingen, Markwiesenstrasse 55, 72770, Reutlingen, Germany.
authors.forename: Ilona
authors.initials: I
authors.lastname: Matiychyn
authors.affiliation: NMI Natural and Medical Sciences Institute, University of Tübingen, Markwiesenstrasse 55, 72770, Reutlingen, Germany. Martin.Stelzle@NMI.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Stelzle
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Weissenborn
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany. Bernhard.Hauer@itb.uni-stuttgart.de.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hauer
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004867:Equipment Design; D057166:High-Throughput Screening Assays; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D053770:Nanowires; D044367:Phase Transition
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D003577:Cytochrome P-450 Enzyme System
keywords: P450; assay; bio-electrochemistry electrode; electrochemistry; enzyme catalysis; nanotubes