title: Transcriptome and selected metabolite analyses reveal points of sugar metabolism in jackfruit (Artocarpus heterophyllus Lam.).
abstract: Artocarpus heterophyllus Lam., commonly known as jackfruit, produces the largest tree-borne fruit known thus far. The edible part of the fruit develops from the perianths, and contains many sugar-derived compounds. However, its sugar metabolism is poorly understood. A fruit perianth transcriptome was sequenced on an Illumina HiSeq 2500 platform, producing 32,459 unigenes with an average length of 1345nt. Sugar metabolism was characterized by comparing expression patterns of genes related to sugar metabolism and evaluating correlations with enzyme activity and sugar accumulation during fruit perianth development. During early development, high expression levels of acid invertases and corresponding enzyme activities were responsible for the rapid utilization of imported sucrose for fruit growth. The differential expression of starch metabolism-related genes and corresponding enzyme activities were responsible for starch accumulated before fruit ripening but decreased during ripening. Sucrose accumulated during ripening, when the expression levels of genes for sucrose synthesis were elevated and high enzyme activity was observed. The comprehensive transcriptome analysis presents fundamental information on sugar metabolism and will be a useful reference for further research on fruit perianth development in jackfruit.
authors.affiliation: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Wanning, Hainan 571533, China. Electronic address: hulis_catas@163.com.
authors.forename: Lisong
authors.initials: L
authors.lastname: Hu
authors.affiliation: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Wanning, Hainan 571533, China. Electronic address: wugang0225@sina.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Wanning, Hainan 571533, China. Electronic address: haochy79@163.com.
authors.forename: Chaoyun
authors.initials: C
authors.lastname: Hao
authors.affiliation: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Wanning, Hainan 571533, China. Electronic address: wangyangyuhuan82@163.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
authors.affiliation: Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Wanning, Hainan 571533, China. Electronic address: t664065649@163.com.
authors.forename: Lehe
authors.initials: L
authors.lastname: Tan
mesh_terms: D031622:Artocarpus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000235:genetics / Q000502:physiology*; D005638:Fruit / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology; D018749:RNA, Plant / Q000302:isolation & purification / Q000502:physiology; D059467:Transcriptome
chemical_list: D018749:RNA, Plant
keywords: Fruit perianth development; Jackfruit; Sugar metabolism; Transcriptome