title: Development of the 19 X-STR loci multiplex system and genetic analysis of a Zhejiang Han population in China.
abstract: The 19 X-STRs multiplex system is a PCR-based amplification kit that facilitates simultaneous amplification of 19 X-chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103,DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X-12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines - Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X-STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing.
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, P. R., China.|Department of Forensic Medicine, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R., China.
authors.forename: XingYi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Criminal Investigation, Zhejiang Provincial Public Security Bureau, Hangzhou, P. R., China.
authors.forename: WeiWei
authors.initials: W
authors.lastname: Wu
authors.affiliation: AGCUScienTch Incorporation, Wuxi, P. R., China.
authors.forename: LinLi
authors.initials: L
authors.lastname: Chen
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, P. R., China.
authors.forename: ChangHui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, P. R., China.|Department of Forensic Medicine, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R., China.
authors.forename: XiaoFang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Forensic Medicine, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R., China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, P. R., China.|Department of Forensic Medicine, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R., China.
authors.forename: XingLin
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Forensic Medicine, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R., China.
authors.forename: HuiJun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou, P. R., China.|Department of Forensic Medicine, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P. R., China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D002681:China; D041321:Chromosomes, Human, X; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005820:Genetic Testing; D005828:Genetics, Population / Q000379:methods*; D006801:Humans; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
keywords: DNA multiplex; Developmental validation; Forensic Science; X short tandem repeat