title: A novel rabies virus lipopeptide provides a better protection by improving the magnitude of DCs activation and T cell responses.
abstract: Besides rabies virus neutralizing antibody, non-neutralizing antibody to internal vital proteins, interferon, and possibly cell-mediated immunity also have a critical role in preventing the infection by rabies virus (RV). We identified novel CTL and Th epitopes which could induce lymphocyte proliferation and IFN-γ, IL-4 production, and designed linear and branched lipopeptides with these selected CTL and Th epitopes. Compared to linear construct, branched lipopeptides, especially Lipo C, stimulate stronger phenotypic and functional maturation of DCs, as well as more efficient CD8(+) T cell responses, evaluating by using FACS, G333-341 tetramer staining and specific CTL assay. Lipo C could also assist rabies vaccine to induce an instant rabies virus neutralizing antibody production, and better protection against rabies virus challenge at early stage. These data reveal that Lipo C could be a promising component for developing novel rabies vaccines.
authors.affiliation: Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Inamullah
authors.initials: I
authors.lastname: Khan
authors.affiliation: Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China. Electronic address: nzhu@fudan.edu.cn.
authors.forename: Naishuo
authors.initials: N
authors.lastname: Zhu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D003601:Cytotoxicity Tests, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D055666:Lipopeptides / Q000276:immunology*; D008807:Mice, Inbred BALB C; D056990:Post-Exposure Prophylaxis / Q000379:methods; D011818:Rabies / Q000517:prevention & control; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology*; D016896:Treatment Outcome; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D018984:Epitopes, T-Lymphocyte; D055666:Lipopeptides; D011819:Rabies Vaccines; D014764:Viral Proteins; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: CTL; Epitope; Lipopeptide; Rabies virus; Vaccine