title: Interleukin 8 serum concentration, but not lactate dehydrogenase activity, positively correlates to CD34 antigen in melanoma tumors.
abstract: CD34 promotes melanoma cell motility, negative regulation of cellular response to hypoxia and positive regulation of vasculogenesis. Interleukin 8 (IL-8) is responsible for angiogenic response in endothelial cells, increases proliferation, metastasis, and survival of melanoma cells. The aim of our study was evaluation of relationship between CD34 immunoexpression and IL-8 serum concentrations in melanoma patients. The study was conducted on patients with melanoma that were divided in: Clark II (17 patients - 19.3%), Clark III (33 patients - 37.5%), Clark IV (22 patients - 25%), and Clark V (16 patients - 18.2%) levels. CD34 expression was absent for Clark II melanomas, and positive for Clark III (6.1%), Clark IV (40.9%), and Clark V (56.2%). The CD34 immune-mark was highly positive only for Clark IV and V levels. Interleukin 8 (IL-8) had high values (above 15 pg/mL) for all patients with melanoma (58.9% - Clark II; 87.8% - Clark III; 90.9% - Clark IV and 93.7% - Clark V). We have found a strong and statistically significant correlation between CD34 and IL-8 for Clark IV (r = 0.54, P < 0.05) and Clark V (r = 0.72, P < 0.05) melanomas. CD34 and IL-8 are associated with poor prognosis of melanoma, metastasis, and neoangiogenesis.
authors.affiliation: a Carol Davila Clinical Hospital of Nephrology , Bucharest , Romania.|b Carol Davila University of Medicine and Pharmacy , Bucharest , Romania.
authors.forename: Corina Daniela
authors.initials: CD
authors.lastname: Ene Nicolae
authors.affiliation: c Victor Babes Hospital of Tropical and Infectious Diseases , Research in Dermatology , Bucharest , Romania.
authors.forename: Ilinca
authors.initials: I
authors.lastname: Nicolae
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000097:blood* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; C508606:CXCL8 protein, human; D016209:Interleukin-8; D007770:L-Lactate Dehydrogenase
keywords: angiogenesis; interleukin 8; lactate dehydrogenase; melanoma; metastasis