title: Phase behavior of lysozyme solutions in the liquid-liquid phase coexistence region at high hydrostatic pressures.
abstract: We present results from small-angle X-ray scattering and turbidity measurements on the effect of high hydrostatic pressure on the phase behavior of dense lysozyme solutions in the liquid-liquid phase separation region, and characterize the underlying intermolecular protein-protein interactions as a function of temperature and pressure under charge-screening conditions (0.5 M NaCl). A reentrant liquid-liquid phase separation region is observed at elevated pressures, which may originate in the pressure dependence of the solvent-mediated protein-protein interaction. A temperature-pressure-concentration phase diagram was constructed for highly concentrated lysozyme solutions over a wide range of temperatures, pressures and protein concentrations including the critical region of the liquid-liquid miscibility gap.
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, D-44221 Dortmund, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Schulze
authors.affiliation: ESRF - The European Synchrotron, 38043 Grenoble, France.
authors.forename: Johannes
authors.initials: J
authors.lastname: Möller
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, D-44221 Dortmund, Germany.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Weine
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, D-44221 Dortmund, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Julius
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, D-44221 Dortmund, Germany.
authors.forename: Nico
authors.initials: N
authors.lastname: König
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, D-44221 Dortmund, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Nase
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, D-44221 Dortmund, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Paulus
authors.affiliation: Fakultät Physik/DELTA, TU Dortmund, D-44221 Dortmund, Germany.
authors.forename: Metin
authors.initials: M
authors.lastname: Tolan
authors.affiliation: Physikalische Chemie, Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn Str. 4a, 44227 Dortmund, Germany. roland.winter@tu-dortmund.de.
authors.forename: Roland
authors.initials: R
authors.lastname: Winter
mesh_terms: D006874:Hydrostatic Pressure; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D009391:Nephelometry and Turbidimetry; D044367:Phase Transition; D060066:Protein Interaction Maps; D053838:Scattering, Small Angle; D012965:Sodium Chloride / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D012996:Solutions; D012965:Sodium Chloride; D009113:Muramidase