title: Inactivation thermodynamics and iso-kinetic profiling for evaluating operational suitability of milk clotting enzyme immobilized in composite polymer matrix.
abstract: Milk clotting enzyme (MCE) was immobilized in alginate-pectate interwoven gel with the yield of 73%. The encapsulated enzyme retained most of the protein load while soluble enzyme lost major proportion of activity after few hours. The immobilized enzyme showed high operational stability by retaining 40% activity even after 10 uses. The narrow optimal working pH of soluble enzyme changed to a broader range after encapsulation and a shift in optimum temperature from 45 to 50°C was also recorded for encapsulated enzyme. Studies on isokinetic temperature showed that immobilized enzyme is more thermo-stable at higher temperature. Immobilization, therefore, not only improved the catalytic properties and stability but also its suitability in food processes like cheese preparation with reduced cost and time.
authors.affiliation: Department of Chemistry & Biochemistry , CCS Haryana Agricultural University, Hisar 125004, India.
authors.forename: Rajesh Kumari
authors.initials: RK
authors.lastname: Narwal
authors.affiliation: ICAR-Central Institute of Post-harvest Engineering & Technology, Abohar 152116, India. Electronic address: buddingbiochemist@gmail.com.
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
authors.affiliation: Department of Chemistry & Biochemistry , CCS Haryana Agricultural University, Hisar 125004, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Pal
authors.affiliation: Department of Chemistry & Biochemistry , CCS Haryana Agricultural University, Hisar 125004, India.
authors.forename: Sarla Popli
authors.initials: SP
authors.lastname: Malhotra
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune 411007, India.
authors.forename: Satish
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Molecular Biology & Biotechnology, Maharana Pratap University of Agriculture and Technology, Udaipur-313001, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Saharan
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology*; D002384:Catalysis; D002417:Cattle; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D006358:Hot Temperature; D007700:Kinetics; D008892:Milk / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized
keywords: Encapsulation; Inactivation thermodynamics; Iso-kinetic profiling; Milk clotting enzyme