title: A Bilobalide-Producing Endophytic Fungus, Pestalotiopsis uvicola from Medicinal Plant Ginkgo biloba.
abstract: For screening bilobalide (BB)-producing endophytic fungi from medicinal plant Ginkgo biloba, a total of 57 fungal isolates were isolated from the internal stem, root, leaf, and bark of the plant G. biloba. Fermentation processes using BB-producing fungi other than G. biloba may become a novel way to produce BB, which is a terpene trilactones exhibiting neuroprotective effects. In this study, a BB-producing endophytic fungal strain GZUYX13 was isolated from the leaves of G. biloba grown in the campus of Guizhou University, Guiyang city, Guizhou province, China. The strain produced BB when grown in potato dextrose liquid medium. The amount of BB produced by this endophytic fungus was quantified to be 106 μg/L via high-performance liquid chromatography (HPLC), substantially lower than that produced by the host tissue. The fungal BB which was analyzed by thin layer chromatography (TLC) and HPLC was proven to be identical to authentic BB. The strain GZUYX13 was identified as Pestalotiopsis uvicola via morphology and ITS rDNA phylogeny. To the best of our knowledge, this is the first report concerning the isolation and identification of endophytic BB-producing Pestalotiopsis spp. from the host plant, which further proved that endophytic fungi have the potential to produce bioactive compounds.
authors.affiliation: Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, 550025, China.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Qian
authors.affiliation: Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, 550025, China. jckang@gzu.edu.cn.|Department of Pharmacy, College of Pharmacy, Guizhou University, Guiyang, 550025, China. jckang@gzu.edu.cn.
authors.forename: Ji-Chuan
authors.initials: JC
authors.lastname: Kang
authors.affiliation: Department of Tobacco Sciences, College of Tobacco Sciences, Guizhou University, Guiyang, 550025, China.
authors.forename: Yi-Kai
authors.initials: YK
authors.lastname: Luo
authors.affiliation: Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, 550025, China.|Department of Pharmacy, College of Pharmacy, Guizhou University, Guiyang, 550025, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhao
authors.affiliation: Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, 550025, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Plant Protection and Quarantine Station of Guiyang, Guiyang, 550081, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Geng
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005663:Furans / Q000378:metabolism*; D020441:Ginkgo biloba / Q000382:microbiology*; D046934:Ginkgolides / Q000378:metabolism*; D018515:Plant Leaves / Q000382:microbiology; D010946:Plants, Medicinal / Q000382:microbiology*; D013098:Xylariales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D005663:Furans; D046934:Ginkgolides; C073710:bilobalide