title: Screening of human bocavirus in surgically excised cancer specimens.
abstract: Human bocavirus (HBoV) is a prevalent virus worldwide and is mainly associated with respiratory disorders. Recently, it was detected in several disease conditions, including cancers. Colorectal cancer (CRC) is the third main cause of cancers worldwide. Risk factors that initiate cell transformation include nutritional, hereditary and infectious causes. The aim of the current study was to screen for the presence of HBoV in solid tumors of colorectal cancer and to determine the genotypes of the detected strains. Surgically excised and paraffin-embedded colorectal cancer tissue specimens from 101 male and female patients with and without metastasis were collected over the last four years. Pathological analysis and tumor stages were determined. The presence of HBoV was screened by polymerase chain reaction, and the genotype of the detected HBoV was determined by direct gene sequencing. Most of the examined specimens were adenocarcinoma with mucinous activity in many of them. Twenty-four out of 101 (23.8 %) CRC tissue specimens were found to contain HBoV-1. Low sequence diversity was recorded in the detected strains. The virus was detected in both male and female patients with an age range of 30-75 years. It is proposed that HBoV-1 could play a potential role in the induction of CRC.
authors.affiliation: College of Medicine, Taif University, Al-Taif, Saudi Arabia. asa@tu.edu.sa.|Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt. asa@tu.edu.sa.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: Abdel-Moneim
authors.affiliation: Department of Surgical Oncology, Faculty of Medicine, Menofia University, Monufia, Egypt.
authors.forename: Hosam A
authors.initials: HA
authors.lastname: El-Fol
authors.affiliation: Department of Clinical Pathology National Cancer Institute, Cairo University, Cairo, Egypt.
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: Kamel
authors.affiliation: Department of Pathology, National Research Institute, Cairo University, Cairo, Egypt.
authors.forename: Ahmed S A
authors.initials: AS
authors.lastname: Soliman
authors.affiliation: Department of Biology, College of Science, Taif University, Al-Taif, Saudi Arabia.|Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
authors.forename: Emad A
authors.initials: EA
authors.lastname: Mahdi
authors.affiliation: Department of Surgical Oncology, Faculty of Medicine, Menofia University, Monufia, Egypt.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: El-Gammal
authors.affiliation: College of Medicine, Taif University, Al-Taif, Saudi Arabia.
authors.forename: Taha Z M
authors.initials: TZ
authors.lastname: Mahran
mesh_terms: D000328:Adult; D000368:Aged; D003106:Colon / Q000473:pathology / Q000601:surgery / Q000821:virology; D015179:Colorectal Neoplasms / Q000473:pathology / Q000601:surgery / Q000821:virology*; D005260:Female; D056661:Human bocavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000473:pathology / Q000601:surgery / Q000821:virology*; D010802:Phylogeny