title: Antibacterial Activity of Carum copticum Essential Oil Against Escherichia Coli O157:H7 in Meat: Stx Genes Expression.
abstract: This work were aimed to (a) determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Carum copticum essential oil (EO) against Escherichia. coli O157:H7 in vitro Trypticase Soy Broth, (TSB) and in ground beef; (b) evaluation of the effect of sub-inhibitory concentrations (sub-MICs) of EO on the growth of bacterium in TSB over 72 h (at 35 °C) and ground beef over 9 days (at 4 °C); and (c) investigation of gene expression involved in Shiga toxins production using relative quantitative real-time PCR method. The MIC in broth and ground beef medium were determined as 0.05 (v/v) and 1.75 % (v/w), respectively. In comparison with control cultures, the EO concentration of 0.03 % in broth caused reduction of colony counting as 1.93, 1.79, and 2.62 log10 CFU ml(-1) after 24, 48, and 72 h at 35 °C, and similarly EO (0.75 %) in ground beef resulted to reduction of colony counting as 1.03, 0.92, 1.48, and 2.12 log10 CFU g (-1) after 2, 5, 7, and 9 days at 4 °C, respectively. An increase and decrease in gene expression were observed as result of EO addition (0.03 %) to broth and (0.5 %) to ground beef was noticed, respectively.
authors.affiliation: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mahmoudzadeh
authors.affiliation: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. hedayat@sbmu.ac.ir.
authors.forename: Hedayat
authors.initials: H
authors.lastname: Hosseini
authors.affiliation: Department of Basic Sciences and Cellular and Molecular Nutrition, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Nasrollahzadeh
authors.affiliation: Department of Food Science, Faculty of Food Engineering, State University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, Caixa Postal: 6121, Campinas, São Paulo, CEP 13083-862, Brazil. mousavi@fea.unicamp.br.
authors.forename: Amin Mousavi
authors.initials: AM
authors.lastname: Khaneghah
authors.affiliation: Department of Basic Sciences and Cellular and Molecular Nutrition, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Marjan
authors.initials: M
authors.lastname: Rismanchi
authors.affiliation: Department of Food Science, Faculty of Food Engineering, State University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, Caixa Postal: 6121, Campinas, São Paulo, CEP 13083-862, Brazil.
authors.forename: Rafael Djalma
authors.initials: RD
authors.lastname: Chaves
authors.affiliation: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Shahraz
authors.affiliation: Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologists, Amol, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Azizkhani
authors.affiliation: Veterinary Faculty, Urmia University, Urmia, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Mahmoudzadeh
authors.affiliation: Department of Nutritional Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Haslberger
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D029974:Carum / Q000737:chemistry*; D002417:Cattle; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005503:Food Additives / Q000494:pharmacology*; D008460:Meat / Q000382:microbiology*; D009822:Oils, Volatile / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D022603:Shiga Toxins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D005503:Food Additives; D009822:Oils, Volatile; D010938:Plant Oils; D022603:Shiga Toxins