title: HDL subpopulations containing apoA-I without apoA-II (LpA-I) in patients with angiographically proven coronary artery disease.
abstract: High density lipoproteins (HDL) can be divided into two metabolically distinct fractions, one containing apolipoprotein (Apo) A-I but not ApoA-II [apolipoprotein A-I; lipoprotein (Lp) A-I] and the other containing both ApoA-I and ApoA-II (LpA-I/A-II). LpA-I fraction which, seeming to be more cardioprotective than LpA-I/A-II particles, is itself heterogeneous. Preβ1-HDL is a minor subfraction of LpA-I and the initial acceptor of cellular cholesterol in the process of reverse cholesterol transport. The aim of the study was to determine the usefulness of the determination of LpA-I fractions as indicators for the atherosclerotic process.
  
  
  
  The study included 112 patients with angiographically-documented coronary artery disease (CAD+) and 51 patients with negative results of coronary angiography (CAD-). We evaluated LpA-I concentration in serum in HDL2and HDL3fractions as well as Preβ1-HDL concentration. Furthermore, we analyzed the association of the assessed parameters with the extent and severity of CAD assessed by Gensini score.
  
  
  
  CAD+ patients were characterized by a lower concentration of serum LpA-I by 19%, LpA-I in HDL2by 26%, higher level of Preβ1-HDL by 27%, and elevated Preβ1-HDL/LpA-I values by 62%. Univariate correlation analysis indicated that serum LpA-I and HDL-cholesterol concentrations were negatively correlated with Gensini score (R=-0.279; p=0.002, R=-0.227; p=0.016, respectively) whereas Preβ1-HDL/LpA-I values were positively correlated with the severity of CAD (R=0.529; p<0.001). In multiple linear regression, after adjusting for age, gender, preexisting hypertension, diabetes, and statin therapy, only the Preβ1-HDL/LpA-I values remained an independent determinant of atherosclerosis severity (β=-0.499; p<0.001).
  
  
  
  Our results show a lower level of LpA-I and higher concentration of Preβ1-HDL in the CAD+ patients compared to the CAD- group. We suggest that the distribution of LpA-I is different in CAD and the Preβ1-HDL/LpA-I ratio may have additional value in assessing anti-atherogenic potential of HDL particles and it is likely to become a clinically valuable indicator of atherosclerosis development.
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Poland. Electronic address: agakuchta@gumed.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kuchta
authors.affiliation: Clinic of Internal Medicine, Connective Tissue Diseases and Geriatrics, Medical University of Gdańsk, Poland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Strzelecki
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ćwiklińska
authors.affiliation: Clinic of Cardiology, Medical University of Gdańsk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Gruchała
authors.affiliation: Clinic of Internal Medicine, Connective Tissue Diseases and Geriatrics, Medical University of Gdańsk, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Zdrojewski
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kortas-Stempak
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Wieczorek
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Gliwińska
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Dąbkowski
authors.affiliation: Department of Clinical Chemistry, Medical University of Gdańsk, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Jankowski
mesh_terms: D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood*; D016633:Apolipoprotein A-II; D008076:Cholesterol, HDL / Q000097:blood*; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood* / Q000000981:diagnostic imaging; D005260:Female; D053442:High-Density Lipoproteins, Pre-beta / Q000097:blood; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D008875:Middle Aged
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D008076:Cholesterol, HDL; D053442:High-Density Lipoproteins, Pre-beta; D008075:Lipoproteins, HDL
keywords: Apolipoprotein A-I; Coronary artery disease; High density lipoprotein; Preβ1-high density lipoprotein