title: Detection of malondialdehyde in processed meat products without interference from the ingredients.
abstract: Our aim was to develop a method for accurate quantification of malondialdehyde (MDA) in meat products. MDA content of uncured ground pork (Control); ground pork cured with sodium nitrite (Nitrite); and ground pork cured with sodium nitrite, sodium chloride, sodium pyrophosphate, maltodextrin, and a sausage seasoning (Mix) was measured by the 2-thiobarbituric acid (TBA) assay with MDA extraction by trichloroacetic acid (method A) and two high-performance liquid chromatography (HPLC) methods: i) HPLC separation of the MDA-dinitrophenyl hydrazine adduct (method B) and ii) HPLC separation of MDA (method C) after MDA extraction with acetonitrile. Methods A and B could not quantify MDA accurately in groups Nitrite and Mix. Nevertheless, MDA in groups Control, Nitrite, and Mix was accurately quantified by method C with good recovery. Therefore, direct MDA quantification by HPLC after MDA extraction with acetonitrile (method C) is useful for accurate measurement of MDA content in processed meat products.
authors.affiliation: Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, South Korea. Electronic address: samooel@cnu.ac.kr.
authors.forename: Samooel
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, South Korea. Electronic address: kichang@scnu.kr.
authors.forename: Ki Chang
authors.initials: KC
authors.lastname: Nam
authors.affiliation: Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, South Korea. Electronic address: cheorun@snu.ac.kr.
authors.forename: Cheorun
authors.initials: C
authors.lastname: Jo
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008315:Malondialdehyde / Q000032:analysis* / Q000737:chemistry*; D008461:Meat Products / Q000032:analysis*; D009573:Nitrites / Q000737:chemistry*; D013552:Swine; D013858:Thiobarbiturates / Q000032:analysis*
chemical_list: D000097:Acetonitriles; D009573:Nitrites; D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid; C032159:acetonitrile
keywords: HPLC; Lipid oxidation; Malondialdehyde; Meat products; Nitrite