title: A novel chimeric influenza virosome containing Vesicular stomatitis G protein as a more efficient gene delivery system.
abstract: OBJECTIVE
  To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome.
  
  
  RESULTS
  A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along with its own hemagglutinin protein. To optimize the transfection efficiency of both chimeric and influenza cationic virosomes, HEK cells were transfected with plasmid DNA and virosomes and the transfection efficiency was assessed by FACS analysis. The chimeric virosome was significantly more efficient in mediating transfection for all amounts of DNA and virosomes compared to the influenza virosome.
  
  
  CONCLUSIONS
  Chimeric influenza virosome, including VSV-G, is superior to the conventional influenza virosome for gene delivery.
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Yahya
authors.initials: Y
authors.lastname: Mohammadzadeh
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Rasouli
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Mohammad Hasan Samiee
authors.initials: MH
authors.lastname: Aref
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Nasim Sadat Seyed
authors.initials: NS
authors.lastname: Tabib
authors.affiliation: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Abdoli
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Peyvand
authors.initials: P
authors.lastname: Biglari
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Saleh
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Tabatabaeian
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran.
authors.forename: Masoumeh Tavassoti
authors.initials: MT
authors.lastname: Kheiri
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran. jamali@pasteur.ac.ir.
authors.forename: Abbas
authors.initials: A
authors.lastname: Jamali
mesh_terms: D018014:Gene Transfer Techniques; D014162:Transfection; D054243:Vesicular Stomatitis / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D022701:Virosomes / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D022701:Virosomes
keywords: Chimeric virosome; Gene delivery system; Hemagglutinin; Influenza virosome; Transfection efficiency; Vesicular stomatitis G protein