title: Comparison of in Vitro Cytotoxicity and Apoptogenic Activity of Magnesium Chloride and Cisplatin as Conventional Chemotherapeutic Agents in the MCF-7 Cell Line.
abstract: Breast cancer is the most common malignancy and also the second leading cause of cancer death among women and also in women that have a high mortality. Previous studies showed that magnesium (Mg) has cytotoxic effects on malignant cell lines. However, the anti-cancer effects of Mg on MCF-7 breast cancer cells are uncertain. This study was aimed at the comparison of the cytotoxic effect of Mg salt (MgCl2) and cisplatin on MCF-7 cells and fibroblasts (as normal cells). After treatment with various concentrations of MgCl2, and cisplatin as a positive control for 24 and 48 hours (h), cytotoxicity activity was measured by MTT assay. In addition, apoptosis was determined by annexin V/propidium iide assay. Both cisplatin and the MgCl2 exhibited dose-dependent cytotoxic effects in the MCF-7 cell line, although the LD50 of the Mg was significantly higher when compared to cispaltin (40 μg/ml vs. 20 μg/ml). Regarding annexin V/propidium results, treatment of MCF-7 cells with LD50 concentrations of cisplatin and Mg showed 59% and 44% apoptosis at 24h, respectively. Finally, the results indicated that Mg has cytotoxic effects on MCF-7 cells, but less than cisplatin as a conventional chemotherapeutic agent. However, regarding the side effects of chemotherapy drugs, it seems that Mg can be considered as a supplement for the treatment of breast cancer.
authors.affiliation: Department of Surgery,Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran E-mail: yekta.parsa@gmail.com.
authors.forename: Seyed Abbas
authors.initials: SA
authors.lastname: Mirmalek
authors.forename: Ehsan
authors.initials: E
authors.lastname: Jangholi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jafari
authors.forename: Soheila
authors.initials: S
authors.lastname: Yadollah-Damavandi
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Javidi
authors.forename: Yekta
authors.initials: Y
authors.lastname: Parsa
authors.forename: Tina
authors.initials: T
authors.lastname: Parsa
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Salimi-Tabatabaee
authors.forename: Hossein
authors.initials: H
authors.lastname: Ghasemzadeh Kolagar
authors.forename: Saeed
authors.initials: S
authors.lastname: Khazaei Jalil
authors.forename: Reza
authors.initials: R
authors.lastname: Alizadeh-Navaei
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D061986:MCF-7 Cells; D015636:Magnesium Chloride / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D015636:Magnesium Chloride; D002945:Cisplatin