title: Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes.
abstract: The present investigation was conducted to study the effects of experimental Clostridium perfringens type D enterotoxaemia in teddy goats. Clinical signs started to appear after 30 min of experimental infection like anorexia, diarrhea, dehydration, frothing and dyspnea. Gross lesions consisted of severe congestion in tissues of varying intensity with enlarged mesenteric lymph nodes while histological examination revealed edema of lungs, kidney, and lymph nodes and to some extent in brain along with hemorrhages in lungs and intestines. Clostridium perfringens type D carrying alpha and epsilon toxin genes were amplified with amplicon size about 247 bp and 665 bp, respectively. Human erythrocytes showed the highest hemolysis, 68%, followed by mice, 57%, against culture supernatants. The percentage of hemolysis was significantly higher at 37°C as compared to 25°C except for rabbit and dog.
authors.affiliation: Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan;
authors.forename: A
authors.initials: A
authors.lastname: Ali Nasir
authors.affiliation: Department of Pathology, College of Veterinary Science, Jhang Campus, Jhang, Pakistan;
authors.forename: M
authors.initials: M
authors.lastname: Younus
authors.affiliation: Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan;
authors.forename: A
authors.initials: A
authors.lastname: Rashid
authors.affiliation: Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan;
authors.forename: S
authors.initials: S
authors.lastname: Abdul Khaliq
authors.affiliation: Department of Pathology, College of Veterinary Science, Jhang Campus, Jhang, Pakistan;
authors.forename: E
authors.initials: E
authors.lastname: Khan
authors.affiliation: Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan;
authors.forename: S H
authors.initials: SH
authors.lastname: Shah
authors.affiliation: Department of Media Sterilization, University of Veterinary and Animal Sciences, Lahore, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Aslam
authors.affiliation: Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan;
authors.forename: M A
authors.initials: MA
authors.lastname: Ghumman
authors.affiliation: Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan;
authors.forename: M H
authors.initials: MH
authors.lastname: Joiya
keywords: Alpha and Epsilon genes; Clostridium perfringens type D; Enterotoxaemia ELISA; Hemolysis