title: Microwave-Assisted Rapid Enzymatic Synthesis of Nucleic Acids.
abstract: Herein we report microwave-induced enhancement of the reactions catalyzed by Escherichia coli DNA polymerase I and avian myeloblastosis virus-reverse transcriptase. The reactions induced by microwaves result in a highly selective synthesis of nucleic acids in 10-50 seconds. In contrast, same reactions failed to give desired reaction products when carried out in the same time periods, but without microwave irradiation. Each of the reactions was carried out for different duration of microwave exposure time to find the optimum reaction time. The products produced by the respective enzyme upon microwave irradiation of the reaction mixtures were identical to that produced by the conventional procedures. As the microwave-assisted reactions are rapid, microwave could be a useful alternative to the conventional and time consuming procedures of enzymatic synthesis of nucleic acids.
authors.affiliation: a CSIR-Institute of Genomics and Integrative Biology , Delhi , India.
authors.forename: Rakha
authors.initials: R
authors.lastname: Hari Das
authors.affiliation: a CSIR-Institute of Genomics and Integrative Biology , Delhi , India.|b Academy of Scientific and Innovative Research Anusandhan Bhawan , New Delhi , India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ahirwar
authors.affiliation: a CSIR-Institute of Genomics and Integrative Biology , Delhi , India.
authors.forename: Saroj
authors.initials: S
authors.lastname: Kumar
authors.affiliation: a CSIR-Institute of Genomics and Integrative Biology , Delhi , India.|b Academy of Scientific and Innovative Research Anusandhan Bhawan , New Delhi , India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Nahar
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D055162:Biocatalysis; D004247:DNA / Q000138:chemical synthesis*; D004256:DNA Polymerase I / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000737:chemistry*; D008872:Microwaves; D012333:RNA, Messenger / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry*; D011980:Receptors, Progesterone / Q000235:genetics; D048348:Reverse Transcription
chemical_list: D029968:Escherichia coli Proteins; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I
keywords: Avian myeloblastosis virus-reverse transcriptase; DNA synthesis; E. coli DNA polymerase I; Microwave; cDNA synthesis