title: Bisphenol A accelerates capacitation-associated protein tyrosine phosphorylation of rat sperm by activating protein kinase A.
abstract: Bisphenol A (BPA) is a synthetic estrogen-mimic chemical. It has been shown to affect many reproductive endpoints. However, the effect of BPA on the mature sperm and the mechanism of its action are not clear yet. Here, our in vitro studies indicated that BPA could accelerate sperm capacitation-associated protein tyrosine phosphorylation in time- and dose-dependent manners. In vivo, the adult male rats exposed to a high dose of BPA could result in a significant increase in sperm activity. Further investigation demonstrated that BPA could accelerate capacitation-associated protein tyrosine phosphorylation even if sperm were incubated in medium devoid of BSA, HCO3 (-), and Ca(2+) However, this action of BPA stimulation could be blocked by H89, a highly selective blocker of protein kinase A (PKA), but not by KH7, a specific inhibitor of adenylyl cyclase. These data suggest that BPA may activate PKA to affect sperm functions and male fertility.
authors.affiliation: Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China University of Chinese Academy of Sciences, Beijing 100864, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wan
authors.affiliation: Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China University of Chinese Academy of Sciences, Beijing 100864, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Ru
authors.affiliation: Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China University of Chinese Academy of Sciences, Beijing 100864, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chu
authors.affiliation: Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Zimei
authors.initials: Z
authors.lastname: Ni
authors.affiliation: Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China zhouych@sibcb.ac.cn ylzhang@sibcb.ac.cn.
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Shoulin
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China zhouych@sibcb.ac.cn ylzhang@sibcb.ac.cn.
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects; D005307:Fertilization in Vitro / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D010636:Phenols / Q000633:toxicity*; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D017868:Cyclic AMP-Dependent Protein Kinases; D007546:Isoquinolines; D010636:Phenols; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D014443:Tyrosine; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: bisphenol A; protein kinase A; sperm; tyrosine phosphorylation