title: Influence of Gender and SNPs in GPX1 Gene on Biomarkers of Selenium Status in Healthy Brazilians.
abstract: Selenium (Se) status varies worldwide as a result of natural variation of Se content in soils, dietary pattern, and the presence of SNPs. Further, Se status in Brazilians and its relationship between genetic variation and Se biomarkers is unknown. This work investigated the association between SNPs in glutathione peroxidase genes and biomarkers of Se status in healthy Brazilians. The study was conducted in 116 healthy adults in São Paulo, Brazil. Plasma and erythrocyte Se were measured by HGFAAS. Erythrocyte GPx (eGPx) activity was measured spectrometrically in a biochemical analyzer. Genotypes were determined by real-time PCR using Taqman(®) Assays. eGPx activity was higher in females compared with males. Lower erythrocyte Se concentrations were found in heterozygous GC carriers for GPX1 rs8179169. eGPx activity was higher in females with the common genotypes, except for rs8179169. GC carriers for rs8179169 had lower erythrocyte Se in both genders, and only male carriers of the variant alleles of both rs1050450 and rs1800668 had higher eGPx activity. In conclusion, the genotype for SNPs in GPX1 and gender affected biomarkers of Se status in this pilot study with healthy Brazilians.
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil. janainalombello@usp.br.
authors.forename: Janaina L S
authors.initials: JL
authors.lastname: Donadio
authors.affiliation: Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil. emguerra@usp.br.
authors.forename: Elvira M
authors.initials: EM
authors.lastname: Guerra-Shinohara
authors.affiliation: Department of Nutrition, Faculty of Public Health, University of São Paulo, São Paulo 01246-904, Brazil. mmrogero@usp.br.
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Rogero
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil. smfcozzo@usp.br.
authors.forename: Silvia M F
authors.initials: SM
authors.lastname: Cozzolino
mesh_terms: D000328:Adult; D001938:Brazil; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005819:Genetic Markers; D005838:Genotype; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D012643:Selenium / Q000097:blood* / Q000378:metabolism; D012737:Sex Factors; D000094765:Glutathione Peroxidase GPX1
chemical_list: D005819:Genetic Markers; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
keywords: biomarkers; glutathione peroxidase (GPx); selenium (Se); single nucleotide polymorphisms (SNPs)