title: Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi.
abstract: The biosynthetic machinery of the first fungal ribosomally synthesized and post-translationally modified peptide (RiPP) ustiloxin B was elucidated through a series of gene inactivation and heterologous expression studies. The results confirmed an essential requirement for novel oxidases possessing the DUF3328 motif for macrocyclization, and highly unique side-chain modifications by three oxidases (UstCF1F2) and a pyridoxal 5'-phosphate (PLP)-dependent enzyme (UstD). These findings provide new insight into the expression of the RiPP gene clusters found in various fungi.
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Japan.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Minami
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Igarashi
authors.affiliation: Biomedicinal Information Research Center (BIRC), Japan Biological Informatics Consortium (JBIC), Koto-ku, Tokyo, 818, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Izumikawa
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan.
authors.forename: Myco
authors.initials: M
authors.lastname: Umemura
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Koto-ku, Tokyo, 135-0064, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Nagano
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, 062-8517, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Machida
authors.affiliation: Biomedicinal Information Research Center (BIRC), Japan Biological Informatics Consortium (JBIC), Koto-ku, Tokyo, 818, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Kawahara
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Koto-ku, Tokyo, 135-0064, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shin-Ya
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Japan. hoik@sci.hokudai.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Oikawa
mesh_terms: D053898:Biosynthetic Pathways; D005658:Fungi / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010456:Peptides, Cyclic / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
chemical_list: D010456:Peptides, Cyclic; C089077:ustiloxin B; D010088:Oxidoreductases
keywords: RiPPs; biosynthesis; heterologous expression; natural products; ustiloxins