title: Streptococcus mutans photoinactivation using a combination of a high potency photopolymerizer and rose bengal.
abstract: BACKGROUND
  Long treatment periods to reach a substantial inactivation of microorganisms are one of the critical challenges in the photodynamic therapy field.
  
  
  METHODS
  Planktonic suspensions of Streptococcus mutans were treated in different groups: presence of rose bengal (RB at 2μM) and light exposure by a new high potency photopolymerizer (L at wavelength=440-480nm; dosimetry=96J/cm(2) - 40s of irradiation; potency density=1200mW/cm(2); dosage=48J) - RB+L+ (PDT), just with dye - RB+L-, just with light - RB-L+ and absence of light and dye RB-L- (control group). Aliquots of each group were transferred to Petri dishes to colony counting (CFU/mL) with the data transformed in log10. The results were submitted to ANOVA and Tukey test at 5%.
  
  
  RESULTS
  PDT group presented total eradication of microorganisms showing statistical difference with all the other groups (5.82log reduction≅99%).
  
  
  CONCLUSIONS
  The high potency photopolymerizer in pulsed emission at an extra short irradiation and low concentration of rose bengal could be considered as a progressive alternative to the control of S. mutans suspensions.
authors.affiliation: Dentistry Program, CEUMA University, R. Josué Montello, n.01, São Luís, 65075120, Brazil. Electronic address: haddaliz@hotmail.com.
authors.forename: Hadda Lyzandra Austríaco
authors.initials: HL
authors.lastname: Leite
authors.affiliation: Master Program in Dentistry, CEUMA University, R. Josué Montello, n.01, São Luís, 65075120, Brazil. Electronic address: salmaaraujo12@hotmail.com.
authors.forename: Salma Ivanna Araújo
authors.initials: SI
authors.lastname: Cavalcante
authors.affiliation: Master Program in Parasitic Biology, CEUMA University, R. Josué Montello, n.01, São Luís, 65075120, Brazil. Electronic address: edmsousa@hotmail.com.
authors.forename: Eduardo Martins
authors.initials: EM
authors.lastname: de Sousa
authors.affiliation: Master Program in Dentistry, CEUMA University, R. Josué Montello, n.01, São Luís, 65075120, Brazil. Electronic address: lets.mg@gmail.com.
authors.forename: Letícia Machado
authors.initials: LM
authors.lastname: Gonçalves
authors.affiliation: Master Program in Dentistry, CEUMA University, R. Josué Montello, n.01, São Luís, 65075120, Brazil. Electronic address: marcobpaschoal@hotmail.com.
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Paschoal
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004359:Drug Therapy, Combination / Q000379:methods; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011829:Radiation Dosage; D012395:Rose Bengal / Q000008:administration & dosage*; D013242:Sterilization / Q000379:methods*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*
chemical_list: D017319:Photosensitizing Agents; D012395:Rose Bengal
keywords: Bacteria; Light; Photochemotherapy; Rose bengal