title: Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines.
abstract: Plant-derived natural products are known to have cancer chemo-preventive and chemo-therapeutic properties. Plant extracts or their active constituents are used as folk medicine in traditional therapies by 80% of the world population. The aim of the present study was to determine the anti-proliferative potential of Fumaria vaillantii extracts on three different cancer cell lines including malignant melanoma SKMEL-3, human breast adenocarcinoma MCF-7 and human myelogenous leukemia K562 as well as human gingival fibroblast (HGF) as normal cell line. Anti-proliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flowcytometry and annexin methods. Total phenolics and flavonoids were determined by Folin-Ciocalteu and aluminum chloride methods. Chloroform fraction had the lowest IC50 value at 72 h (0.1 μg/ml) in MCF-7 cells. Flowcytometry and annexin-V analysis indicated that the chloroform fraction induced necrosis in MCF-7 cells. In addition, the colorimetric methods showed that the methanolic fraction possessed the highest amount of total phenolics (33.03 ± 0.75 mg/g of dry powder) and flavonoids (10.5 ± 2.0 mg/g of dry powder). The collective data demonstrated that F. vaillantii chloroform fraction may contain effective compounds with chemo-therapeutic potential act through an apoptotic independent pathway.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, I.R. Iran.
authors.forename: Fatemeh Haji Abbas
authors.initials: FH
authors.lastname: Tabrizi
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, I.R. Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Irian
authors.affiliation: National Cell Bank of Pasteur Institute of Iran, Tehran, I.R. Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Amanzadeh
authors.affiliation: National Cell Bank of Pasteur Institute of Iran, Tehran, I.R. Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Heidarnejad
authors.affiliation: Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, I.R. Iran.
authors.forename: Hoda
authors.initials: H
authors.lastname: Gudarzi
authors.affiliation: Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, I.R. Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Salimi
keywords: Apoptosis; Cancer; Flowcytometry; Fumaria vaillantii; MTT assay