title: Long-term in vivo single-cell tracking reveals the switch of migration patterns in adult-born juxtaglomerular cells of the mouse olfactory bulb.
abstract: The behavior of adult-born cells can be easily monitored in cell culture or in lower model organisms, but longitudinal observation of individual mammalian adult-born cells in their native microenvironment still proves to be a challenge. Here we have established an approach named optical cell positioning system for long-term in vivo single-cell tracking, which integrates red-green-blue cell labeling with repeated angiography. By combining this approach with in vivo two-photon imaging technique, we characterized the in vivo migration patterns of adult-born neurons in the olfactory bulb. In contrast to the traditional view of mere radial migration of adult-born cells within the bulb, we found that juxtaglomerular cells switch from radial migration to long distance lateral migration upon arrival in their destination layer. This unique long-distance lateral migration has characteristic temporal (stop-and-go) and spatial (migratory, unidirectional or multidirectional) patterns, with a clear cell age-dependent decrease in the migration speed. The active migration of adult-born cells coincides with the time period of initial fate determination and is likely to impact on the integration sites of adult-born cells, their odor responsiveness, as well as their survival rate.
authors.affiliation: Institute of Physiology II, University of Tübingen, 72074 Tübingen, Germany.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Institute of Physiology II, University of Tübingen, 72074 Tübingen, Germany.
authors.forename: Kaizhen
authors.initials: K
authors.lastname: Li
authors.affiliation: Research Department of Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Riecken
authors.affiliation: Institute of Physiology II, University of Tübingen, 72074 Tübingen, Germany.
authors.forename: Anatoliy
authors.initials: A
authors.lastname: Maslyukov
authors.affiliation: Centre for Biological Sciences, University of Southampton, SO166YD Southampton, UK.
authors.forename: Diego
authors.initials: D
authors.lastname: Gomez-Nicola
authors.affiliation: Institute of Physiology II, University of Tübingen, 72074 Tübingen, Germany.
authors.forename: Yury
authors.initials: Y
authors.lastname: Kovalchuk
authors.affiliation: Research Department of Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
authors.forename: Boris
authors.initials: B
authors.lastname: Fehse
authors.affiliation: Institute of Physiology II, University of Tübingen, 72074 Tübingen, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Garaschuk
mesh_terms: D000818:Animals; D002465:Cell Movement; D058948:Cell Tracking; D002478:Cells, Cultured; D057809:HEK293 Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology*
chemical_list: D008164:Luminescent Proteins