title: Oncomirs miRNA-221/222 and Tumor Suppressors miRNA-199a/195 Are Crucial miRNAs in Liver Cancer: A Systematic Analysis.
abstract: The high mortality rate of hepatocellular carcinoma (HCC) is partly due to a lack of good diagnostic markers and treatment strategies. Recently, several microRNA (miRNA) profiling studies were conducted with HCC; however, their inconsistency means that their diagnostic or therapeutic value is debatable.
  
  
  
  This study aims to systematically evaluate the consistency of miRNAs from multiple independent studies.
  
  
  
  A systematic analysis of miRNAs from eligible publications was conducted, followed by real-time PCRs. The targets of highly consistent miRNAs were collected using online programs, followed by enrichment analyses for gene ontology terms and Kyoto encyclopedia of genes and genomes pathways.
  
  
  
  In total, 241 differentially expressed miRNAs were reported in 13 HCC profiling studies, of which 137 were upregulated and 104 downregulated. Among consistently upregulated miRNAs (cutoff > fourfold), miRNA-222, miRNA-21, miRNA-221, miRNA-210, and miRNA-224 were found increased in 8, 6, 6, 5, and 5 different studies, respectively. Among 137 downregulated miRNAs, miRNA-195, miRNA-199a, miRNA-125b, and miRNA-99a were reported in 8, 8, 5, and 5 studies, respectively. These results were confirmed by real-time PCR. Enrichment analyses demonstrated that programmed cell death and proliferation play important roles during the interplay of miRNA with HCC.
  
  
  
  miRNAs most consistently related to HCC are oncomirs miRNA-221/222 and tumor suppressors miRNA-199a/195.
authors.affiliation: College of Life Science, Shihezi University, 48 Dongmingxin St., Urumqi, 830011, Xinjiang, People's Republic of China.|School of Medicine, Hangzhou Normal University, 16 Xuelin Road, Xiasha Higher Education Park, Hangzhou, 310036, Zhejiang, People's Republic of China.
authors.forename: Yanhu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou St., Hangzhou, 310015, Zhejiang, People's Republic of China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Di
authors.affiliation: IHRC Inc., 2 Ravinia Drive, Suite 1750, Atlanta, GA, 30346, USA.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou St., Hangzhou, 310015, Zhejiang, People's Republic of China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Cai
authors.affiliation: Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou St., Hangzhou, 310015, Zhejiang, People's Republic of China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Tan
authors.affiliation: Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou St., Hangzhou, 310015, Zhejiang, People's Republic of China.
authors.forename: Liangwen
authors.initials: L
authors.lastname: Xu
authors.affiliation: Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou St., Hangzhou, 310015, Zhejiang, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou St., Hangzhou, 310015, Zhejiang, People's Republic of China. Louguoqiang2012@126.com.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Lou
authors.affiliation: Affiliated Hospital, Hangzhou Normal University, 126 Wenzhou St., Hangzhou, 310015, Zhejiang, People's Republic of China. yutaoyan@yahoo.com.|School of Medicine, Hangzhou Normal University, 16 Xuelin Road, Xiasha Higher Education Park, Hangzhou, 310036, Zhejiang, People's Republic of China. yutaoyan@yahoo.com.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Yan
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D035683:MicroRNAs; C552722:MIRN195 microRNA, human; C524940:MIRN21 microRNA, human; C525709:MIR221, human; C525710:MIR222, human; C546140:MIRN224 microRNA, human; C561679:MIRN99 microRNA, human; C531408:mirn199 microRNA, human
keywords: Differential expression; Hepatocellular carcinoma; Systematic study; miRNA