title: Temperature induced modulation of lipid oxidation and lipid accumulation in palmitate-mediated 3T3-L1 adipocytes and 3T3-L1 adipocytes.
abstract: Human skin temperature can vary widely depending on anatomical location and ambient temperature. It is also known that local changes in skin and subcutaneous temperature can affect fat metabolism. This study aimed to explore the potential effects of surrounding thermal environment on fat by investigating cell viability, lipid oxidation, and lipid accumulation in 3T3-L1 adipocytes and palmitate-treated adipocytes after 4h incubation. No significant differences of viability in 3T3-L1 adipocytes were detected under different temperature conditions. Despite no significant increase being observed under warm temperature (39°C) conditions, a similarly significant suppression of intracellular reactive oxygen species (ROS) and lipid peroxidation were found in 3T3-L1 adipocytes and palmitate-treated adipocytes under 4h exposure to cooler temperatures of 31-33°C (P<0.01). ROS, chemically reactive molecules containing oxygen, are currently understood to be a major contributor to oxidantive stress in obesity. Additionally, cooler temperatures (31-33°C) could improve the size of lipid droplets in 3T3-L1 adipocytes (P<0.01), but no significant effect was generated by temperature change on lipid droplets in palmitate-treated adipocytes. In the palmitate-induced adiposity model, although excessive ROS and lipid peroxidation has been attenuated by temperature decrease (P<0.01), it still does not positively modulate lipid droplet size (P>0.05) and remedy the palmitate damage induced cell death (P<0.01). These findings provide preliminary support for potential interventions based on temperature manipulation for cell metabolism of adipocytes.
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, United Kingdom.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Lin
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, United Kingdom. Electronic address: henry.yili@manchester.ac.uk.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Polly Hangmei
authors.initials: PH
authors.lastname: Leung
authors.affiliation: School of Materials, The University of Manchester, Manchester M13 9PL, United Kingdom.
authors.forename: Jiashen
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Junyan
authors.initials: J
authors.lastname: Hu
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival; D066292:Lipid Droplets / Q000378:metabolism; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D051379:Mice; D010168:Palmitates / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013696:Temperature
chemical_list: D010168:Palmitates; D017382:Reactive Oxygen Species
keywords: Adipocytes; Cell viability; Lipid droplet size; Oxidative stress; Temperature