title: [THE TECHNIQUE OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR SIMULTANEOUS DIAGNOSTIC OF INHERENT HYPERPLASIA OF ADRENAL GLANDS TYPE I AND II].
abstract: The article considers the technique of high-performance liquid chromatography making it possible simultaneously detect cortisol, cortisone and secondary steroids in serum for consequent analysis of common reversed-phase high-performance liquid chromatography with ultraviolet under 240 nm. The liquid-liquid extraction from alkaline medium in diethyl ether The separation using column of 150x4.6 size ODS 3.5 mkm in isocratic mode. The eluent acetonitrile--0.02 M phosphate buffer pH 8.0--isopropanol (40:60:1). The application of proposed technique managed to separate cortisol, cortisone, dexamethasone, corticosterone, 11-desoxicortisol, testosterone, desoxicorticosterone, 17α-gidroxiprogesterone and androstendion in 20 minutes. The simplicity, reproducibility and sufficient selectivity and sensitivity of technique permit implement it in clinical practice for simultaneous diagnostic of inherent hyperplasia of adrenal glands type I and II.
authors.forename: A A
authors.initials: AA
authors.lastname: Dutov
authors.forename: D A
authors.initials: DA
authors.lastname: Nikitin
authors.forename: Yu L
authors.initials: YL
authors.lastname: Lukyanova
authors.forename: N A
authors.initials: NA
authors.lastname: Shemiakina
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000311:Adrenal Glands / Q000378:metabolism / Q000473:pathology*; D000735:Androstenedione / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003345:Corticosterone / Q000097:blood; D003348:Cortisone / Q000097:blood*; D003350:Cortodoxone / Q000097:blood; D003900:Desoxycorticosterone / Q000097:blood; D003907:Dexamethasone / Q000097:blood; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D006908:Hydroxyprogesterones / Q000097:blood; D006965:Hyperplasia / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000097:blood
chemical_list: D000097:Acetonitriles; D006908:Hydroxyprogesterones; C030228:11-hydroxyprogesterone; D013739:Testosterone; D000735:Androstenedione; D003900:Desoxycorticosterone; D003907:Dexamethasone; D003348:Cortisone; D003345:Corticosterone; D003350:Cortodoxone; D006854:Hydrocortisone; C032159:acetonitrile