title: DNA barcoding and phylogeny of Calidris and Tringa (Aves: Scolopacidae).
abstract: The avian genera Calidris and Tringa are the largest of the widespread family of Scolopacidae. The phylogeny of members of the two genera is still a matter of controversial. Mitochondrial cytochrome c oxidase subunit I (COI) can serve as a fast and accurate marker for the identification and phylogeny of animal species. In this study, we analyzed the COI barcodes of thirty-one species of the two genera. All the species had distinct COI sequences. Two hundred and twenty-one variable sites were identified. Kimura two-parameter distances were calculated between barcodes. Neighbor-joining and maximum likelihood methods were used to construct phylogenetic trees. All the species could be discriminated by their distinct clades in the phylogenetic trees. The phylogenetic trees grouped all the species of Calidris and Tringa into different monophyletic clade, respectively. COI data showed a well-supported phylogeny for Calidris and Tringa species.
authors.affiliation: a School of Life Sciences , Jinggangshan University , Ji'an , Jiangxi Province , China.
authors.forename: Zuhao
authors.initials: Z
authors.lastname: Huang
authors.affiliation: b Jiangxi Academy of Forestry , Nanchang , Jiangxi Province , PR China.
authors.forename: Feiyun
authors.initials: F
authors.lastname: Tu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics; D001717:Birds / Q000145:classification* / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics; D010802:Phylogeny
chemical_list: D030161:Avian Proteins; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV
keywords: COI; Calidris; DNA barcoding; Tringa; phylogeny