title: A single mouse monoclonal antibody, E58 modulates multiple IgE epitopes on group 1 cedar pollen allergens.
abstract: We recently described a dominant role for conformational epitopes on the group 1 allergen of the mountain cedar (Juniperus ashei, Cupressaceae), Jun a 1, in pollen hypersensitivity in South Central U.S.A. Since these epitopes are surface exposed and are likely to be flexible, they may be susceptible to molecular or physical perturbations. This may make Jun a 1 a potential target for new forms of therapy for cedar pollinosis. Here, we describe a mouse monoclonal antibody, termed E58, which binds to the group 1 allergens of the cedar pollens from three highly populated regions of the world (central U.S.A., France and Japan). Upon binding to these allergens, E58 strongly reduces the binding of patient's IgE antibodies to these dominant allergens. This characteristic of E58, and potentially other similar antibodies, suggests an opportunity to develop preventative or therapeutic agents that may inhibit cedar pollen sensitization or prevent their allergic reactions.
authors.affiliation: Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0366, USA; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-1068, USA.
authors.forename: Randall M
authors.initials: RM
authors.lastname: Goldblum
authors.affiliation: Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0366, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Ning
authors.affiliation: Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0366, USA.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Judy
authors.affiliation: Sealy Center for Structural Biology and Molecular Biophysics, Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-1068, USA.
authors.forename: Luis Marcelo F
authors.initials: LM
authors.lastname: Holthauzen
authors.affiliation: Isis Clinical Research, LLC, 6836 Austin Center Blvd. Ste 180, Austin, TX 78731, USA.
authors.forename: Julius
authors.initials: J
authors.lastname: van Bavel
authors.affiliation: Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0366, USA.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kamijo
authors.affiliation: Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0366, USA. Electronic address: tmidoro@utmb.edu.
authors.forename: Terumi
authors.initials: T
authors.lastname: Midoro-Horiuti
mesh_terms: D000485:Allergens; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D052179:Antigens, Plant / Q000276:immunology*; D028203:Cedrus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D051379:Mice; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D020349:Surface Plasmon Resonance
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D018985:Epitopes, B-Lymphocyte; D010940:Plant Proteins; C121951:jun a 1 protein, Juniperus ashei; D007073:Immunoglobulin E
keywords: Allergen structure; Cedar pollen allergy; Conformational epitope; IgE epitope; Jun a 1; Monoclonal antibody