title: Fluorescent Cell Imaging in Regenerative Medicine.
abstract: Fluorescent protein imaging, a promising tool in biological research, incorporates numerous applications that can be of specific use in the field of regenerative medicine. To enhance tissue regeneration efforts, scientists have been developing new ways to monitor tissue development and maturation in vitro and in vivo. To that end, new imaging tools and novel fluorescent proteins have been developed for the purpose of performing deep-tissue high-resolution imaging. These new methods, such as intra-vital microscopy and Förster resonance energy transfer, are providing new insights into cellular behavior, including cell migration, morphology, and phenotypic changes in a dynamic environment. Such applications, combined with multimodal imaging, significantly expand the utility of fluorescent protein imaging in research and clinical applications of regenerative medicine.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.; Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest Baptist Health, Winston-Salem, NC, USA.
authors.forename: Etai
authors.initials: E
authors.lastname: Sapoznik
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.
authors.forename: Guoguang
authors.initials: G
authors.lastname: Niu
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.
authors.forename: Sean V
authors.initials: SV
authors.lastname: Murphy
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, USA.; Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest Baptist Health, Winston-Salem, NC, USA.
authors.forename: Shay
authors.initials: S
authors.lastname: Soker
keywords: fluorescent protein; live-cell imaging; microscopy; regenerative medicine; tissue engineering