title: [Effects of cyclooxygenase-2 selective inhibitor celecoxib on the expression of major vault protein in rats with status epilepticus].
abstract: OBJECTIVE
  To study the effect of cyclooxygenase -2 selective inhibitor celecoxib on the expression of major vault protein ( MVP) in the brain of rats with status epilepticus and its possible roles in the treatment of refractory epilepsy.
  
  
  METHODS
  Sixty adult male Sprague-Dawley rats were randomly assigned to blank control (n=16), epilepsy model (n=22) and celecoxib treatment groups (n=22). After the status epilepticus was induced in rats by injecting lithium and pilocarpine, each group had 16 rats enrolled as subjects. Immunohistochemical method and Western blot method were used to detect the expression of MVP in the frontal cortex and hippocampus.
  
  
  RESULTS
  The expression of MVP was significantly higher in the epilepsy model group than in the control group (P<0.01). The expression of MVP in the celecoxib treatment group was significantly decreased compared with the epilepsy model group, but it was still higher than in the control group (P<0.01).
  
  
  CONCLUSIONS
  Celecoxib could decrease the expression of MVP in brain tissue of rats with status epilepticus, suggesting that it is promising for the treatment of intractable epilepsy.
authors.affiliation: Department of Pediatrics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. runningtortoise@163.com.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Song
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Jiang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D000068579:Celecoxib / Q000494:pharmacology* / Q000627:therapeutic use; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000188:drug therapy* / Q000378:metabolism; D020394:Vault Ribonucleoprotein Particles / Q000032:analysis*; D000099187:Major Vault Protein
chemical_list: D000068579:Celecoxib; D052246:Cyclooxygenase 2 Inhibitors; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein