title: Plantago lagopus B Chromosome Is Enriched in 5S rDNA-Derived Satellite DNA.
abstract: B chromosomes are supernumerary dispensable parts of the karyotype which appear in some individuals of some populations in some species. Using advanced sequencing technology, we in silico characterized the high-copy DNA composition of Plantago lagopus with and without B chromosomes. The nuclear genome (2.46 pg/2C) was found to be relatively rich in repetitive sequences, with highly and moderately repeated elements making up 68% of the genome. Besides a centromere-specific marker, we identified a B-specific satellite and a repeat enriched in polymorphic A chromosome segments. The B-specific tandem repeat PLsatB originated from sequence amplification including 5S rDNA fragments.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Stadt Seeland, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Kumke
authors.forename: Jiří
authors.initials: J
authors.lastname: Macas
authors.forename: Jörg
authors.initials: J
authors.lastname: Fuchs
authors.forename: Lothar
authors.initials: L
authors.lastname: Altschmied
authors.forename: Jasmeet
authors.initials: J
authors.lastname: Kour
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Dhar
authors.forename: Andreas
authors.initials: A
authors.lastname: Houben
mesh_terms: D002503:Centromere / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D003198:Computer Simulation; D004275:DNA, Ribosomal / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D010943:Plantago / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D004276:DNA, Satellite; D012341:RNA, Ribosomal, 5S