title: Communication of cAMP by connexin43 gap junctions regulates osteoblast signaling and gene expression.
abstract: Connexin43 (Cx43) containing gap junctions play an important role in bone homeostasis, yet little is known about the second messengers communicated by Cx43 among bone cells. Here, we used MC3T3-E1 pre-osteoblasts and UMR106 rat osteosarcoma cells to test the hypothesis that cAMP is a second messenger communicated by bone cells through Cx43 containing gap junctions in a manner that is sufficient to impact osteoblast function. Overexpression of Cx43 markedly enhanced the activity of a cAMP-response element driven transcriptional luciferase reporter (CRE-luc) and increased phospho-CREB and phospho-ERK1/2 levels following expression of a constitutively active Gsα or by treatment with prostaglandin E2 (PGE2), 3-Isobutyl-1-methyl xanthine (IBMX) or forskolin. The Cx43-dependent potentiation of signaling in PGE2 treated cells was not accompanied by a further increase in cAMP levels, suggesting that the cAMP was shared between cells rather than Cx43 enhancing cAMP production. To support this, we developed a novel assay in which one set of cells expressing constitutively active Gsα (donor cells) were co-cultured with a second set of cells expressing a CRE-luc reporter (acceptor cells). Using this assay, activation of a CRE-luc reporter in the acceptor cells was both Cx43- and cell contact-dependent, indicating communication of cAMP among cells. Finally, we showed that Cx43 increased the cAMP-dependent mRNA expression of receptor activator of nuclear factor kappa B ligand (RANKL) and enhanced the repression of the sclerostin mRNA, implying a potential mechanism for the modulation of tissue remodeling. In total, these data demonstrate that Cx43 can communicate cAMP between cells and, more importantly, that the communicated cAMP is sufficient to impact signal transduction cascades and the expression of key bone effector molecules between interconnected cells.
authors.affiliation: Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Aditi
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Hidayah
authors.initials: H
authors.lastname: Anderson
authors.affiliation: Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Atum M
authors.initials: AM
authors.lastname: Buo
authors.affiliation: Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Megan C
authors.initials: MC
authors.lastname: Moorer
authors.affiliation: Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Margaret
authors.initials: M
authors.lastname: Ren
authors.affiliation: Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD, USA. Electronic address: jstains@som.umaryland.edu.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Stains
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D045744:Cell Line, Tumor; D018031:Connexin 43 / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism*; D005786:Gene Expression Regulation; D006706:Homeostasis / Q000235:genetics; D051379:Mice; D050505:Mutant Proteins / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D015398:Signal Transduction
chemical_list: D018031:Connexin 43; D017362:Cyclic AMP Response Element-Binding Protein; D050505:Mutant Proteins; D000242:Cyclic AMP; D015232:Dinoprostone
keywords: Connexin; Cyclic AMP (cAMP); G protein; Gap junction; Osteoblast; Prostaglandin E2