title: Detection of Human Papillomavirus Genotypes and Major BRCA Mutations in Familial Breast Cancer.
abstract: Breast cancer is a multistep disease and infection with a DNA virus could play a role in one or more of the steps in this pathogenic process. High-risk human papillomaviruses (HPVs) are the causative agents of several cancers. In this study, we investigated HPV genotypes associated with breast cancer and its relationship with BRCA mutation for the detection of familial breast cancer. We analyzed 84 formalin-fixed, paraffin-embedded tissue blocks from 38 familial breast cancer and 46 nonfamilial breast cancer samples by multiplex polymerase chain reaction and clinical parameters. Overall prevalence of HPV infection was 27 of 84: 10 (37.03%) HPV-16, 9 (29.62%) HPV-18, 4 (14.81%) HPV-11, 1 (3.7%) HPV-31, 1 (3.7%) HPV-33, and 2 (7.4%) HPV35. Furthermore, 17 mtDNA4977 deletions and 5 5382insC mutations were detected from 38 familial breast cancer samples. Our results demonstrate that infection with HPV was prevalent among Iranian women with familial breast cancer and the testing of mtDNA4977 deletions and 5382insC mutations in combination with clinical parameters as major risk factors can serve in the identification of familial breast cancer.
authors.affiliation: 1 Biotechnology Academy of Gene Gostar , Karaj, Iran .
authors.forename: Parinaz
authors.initials: P
authors.lastname: Mohtasebi
authors.affiliation: 2 Department of Biology, College of Basic Sciences, Karaj Branch, Islamic Azad University , Alborz, Iran .
authors.forename: Hossein
authors.initials: H
authors.lastname: Rassi
authors.affiliation: 2 Department of Biology, College of Basic Sciences, Karaj Branch, Islamic Azad University , Alborz, Iran .
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Maleki
authors.affiliation: 1 Biotechnology Academy of Gene Gostar , Karaj, Iran .
authors.forename: Sameh
authors.initials: S
authors.lastname: Hajimohammadi
authors.affiliation: 2 Department of Biology, College of Basic Sciences, Karaj Branch, Islamic Azad University , Alborz, Iran .
authors.forename: Zahra
authors.initials: Z
authors.lastname: Bagheri
authors.affiliation: 1 Biotechnology Academy of Gene Gostar , Karaj, Iran .
authors.forename: Malihe
authors.initials: M
authors.lastname: Fakhar Miandoab
authors.affiliation: 1 Biotechnology Academy of Gene Gostar , Karaj, Iran .
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Naserbakht
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019313:BRCA1 Protein / Q000235:genetics*; D024682:BRCA2 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology / Q000821:virology; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007492:Iran; D008875:Middle Aged; D009154:Mutation; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics / Q000821:virology
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D004272:DNA, Mitochondrial