title: A real-time polymerase chain reaction method for the identification of four commercially important salmon and trout species.
abstract: A duplex quantitative real-time PCR (qPCR) assay was developed for rapid and accurate identification of four commercially important salmon and trout species (Oncorhynchus keta, Oncorhynchus nerka, Oncorhynchus mykiss, and Salmo salar) commonly used for production process of fish in China. The assays targeting the mitochondrial control region (CR) and 16S rRNA gene were able to simultaneously discriminate four target species and the family Salmonidae from processed as well as fresh fish. The qPCR efficiency of each reaction was calculated according to the standard curve, and the method was validated by amplification DNA extracted from single or artificial mixtures prepared with the reference salmon and trout species. Testing of 11 commercial salmon and trout products by the established qPCR assay demonstrated that it was really a useful and academic technique to identify four commercially important salmon and trout species.
authors.affiliation: a Institute of Seafood, Zhejiang Gongshang University , Hangzhou , Zhejiang , PR China.
authors.forename: Junli
authors.initials: J
authors.lastname: Feng
authors.affiliation: b Zhejiang Entry-Exit Inspection and Quarantine Bureau , Hangzhou , Zhejiang , PR China , and.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: b Zhejiang Entry-Exit Inspection and Quarantine Bureau , Hangzhou , Zhejiang , PR China , and.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xie
authors.affiliation: a Institute of Seafood, Zhejiang Gongshang University , Hangzhou , Zhejiang , PR China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Dai
authors.affiliation: c Institute of Bioengineering, Zhejiang Sci-Tech University , Hangzhou , Zhejiang , PR China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004272:DNA, Mitochondrial; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S; D012045:Regulatory Sequences, Nucleic Acid; D012474:Salmon / Q000145:classification* / Q000235:genetics; D014337:Trout / Q000145:classification* / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S
keywords: Control region; quantitative real-time PCR; salmon; species identification