title: DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis.
abstract: High-quality human DNA samples and associated information of individuals are necessary for biomedical research. Biobanks act as a support infrastructure for the scientific community by providing a large number of high-quality biological samples for specific downstream applications. For this purpose, biobank methods for sample preparation must ensure the usefulness and long-term functionality of the products obtained. Quality indicators are the tool to measure these parameters, the purity and integrity determination being those specifically used for DNA. This study analyzes the quality indicators in DNA samples derived from 118 frozen human tissues in optimal cutting temperature (OCT) reactive, 68 formalin-fixed paraffin-embedded (FFPE) tissues, 119 frozen blood samples, and 26 saliva samples. The results obtained for DNA quality are discussed in association with the usefulness for downstream applications and availability of the DNA source in the target study. In brief, if any material is valid, blood is the most approachable option of prospective collection of samples providing high-quality DNA. However, if diseased tissue is a requisite or samples are available, the recommended source of DNA would be frozen tissue. These conclusions will determine the best source of DNA, according to the planned downstream application. Furthermore our results support the conclusion that a complete procedure of DNA quantification and qualification is necessary to guarantee the appropriate management of the samples, avoiding low confidence results, high costs, and a waste of samples.
authors.affiliation: 1 Andalusian Public Health System Biobank, Armilla, Spain .|2 Instituto de Investigación Biosanitaria de Granada , Armilla, Spain .
authors.forename: Gema
authors.initials: G
authors.lastname: Lucena-Aguilar
authors.affiliation: 1 Andalusian Public Health System Biobank, Armilla, Spain .|2 Instituto de Investigación Biosanitaria de Granada , Armilla, Spain .
authors.forename: Ana María
authors.initials: AM
authors.lastname: Sánchez-López
authors.affiliation: 1 Andalusian Public Health System Biobank, Armilla, Spain .
authors.forename: Cristina
authors.initials: C
authors.lastname: Barberán-Aceituno
authors.affiliation: 1 Andalusian Public Health System Biobank, Armilla, Spain .|2 Instituto de Investigación Biosanitaria de Granada , Armilla, Spain .
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Carrillo-Ávila
authors.affiliation: 3 Laboratory of Molecular Biology, Fundación Instituto Valenciano de Oncología , Valencia, Spain .|4 Biobank, Fundación Instituto Valenciano de Oncología , Valencia, Spain .
authors.forename: José Antonio
authors.initials: JA
authors.lastname: López-Guerrero
authors.affiliation: 1 Andalusian Public Health System Biobank, Armilla, Spain .|2 Instituto de Investigación Biosanitaria de Granada , Armilla, Spain .
authors.forename: Rocío
authors.initials: R
authors.lastname: Aguilar-Quesada
mesh_terms: D018070:Biological Specimen Banks / Q000592:standards; D015925:Cryopreservation / Q000379:methods / Q000592:standards*; D004247:DNA / Q000032:analysis / Q000592:standards*; D005557:Formaldehyde; D020869:Gene Expression Profiling; D006801:Humans; D016612:Paraffin Embedding; D011446:Prospective Studies; D016707:Tissue Fixation / Q000379:methods / Q000592:standards*
chemical_list: D005557:Formaldehyde; D004247:DNA