title: [THE MOLECULAR TECHNIQUES OF DIAGNOSTIC OF GINGIVITIS AND PERIODONTITIS IN HIV-INFECTED PATIENTS].
abstract: The examination was carried out in the Moscow clinical infectious hospital No 2 concerning 102 patients with verified diagnosis "AIDS-infection" and seropositive according results of detection of anti-HIV-antibodies in blood serum. The study was organized to analyze rate ofcolonization of gums with virulent anaerobic bacteria in HIV-infected (polymerase chain reaction) and antibodies to HIV in gingival fluid (enzyme-linked immunosorbent assay). It is established that in HIV-infected patients, in scrape from gingival sulcus dominate anaerobic bacteria P. gigngivalis and A. ctinomycetemcomitans and in case of periodontitis--P. gingivalis and T. forsythia. The received data permits recommending the test-system "Multident-5" for polymerase chain reaction diagnostic. The reagents kit "Calypte®HIV-1/2"--for enzyme-linked immunosorbent assay gingival fluid. The results of polymerase chain reaction and enzyme-linked immunosorbent assay have no impact of concomitant stomatological (periodontitis, gingivitis) and somatic pathology.
authors.forename: V N
authors.initials: VN
authors.lastname: Tsarev
authors.forename: E N
authors.initials: EN
authors.lastname: Nikolaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Iagodina
authors.forename: Yu A
authors.initials: YA
authors.lastname: Trefilova
authors.forename: E V
authors.initials: EV
authors.lastname: Ippolitov
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005891:Gingivitis / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D015658:HIV Infections / Q000150:complications / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development / Q000472:pathogenicity; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic / Q000382:microbiology*; D048373:Treponema denticola / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D011933:Reagent Kits, Diagnostic