title: Sleep restriction reduces the survival time and aggravates the neurological dysfunction and memory impairments in an animal model of cerebral hypoperfusion.
abstract: Cerebral blood flow is associated with the cerebrovascular prognosis. Sleep restriction (SR) may be a limiting factor of the prognosis after a cerebrovascular event, impairing the neurological recovery. We aimed to investigate the effects of SR on mortality rate and on behavioral and histological parameters of animals submitted to permanent cerebral hypoperfusion. Sixty male Wistar rats were distributed in 4 groups, according to the protocol of common carotid artery occlusion (CCAO) and SR: nSR+nCCAO, SR+nCCAO, nSR+CCAO, and SR+CCAO. The groups SR+nCCAO and SR+CCAO were submitted to SR during 10 days. The cerebral hypoperfusion was induced by the permanent CCAO. Neurological function and memory were assessed over 14 days of cerebral hypoperfusion. Analysis of neuropathological alterations were performed in the CA1 region of hippocampus. The mortality rate was 40% in the nSR+CCAO and SR+CCAO groups. SR significantly reduced the survival time of animals submitted to CCAO. After 7 and 14 days of cerebral hypoperfusion, 11% and 33% of the nSR+CCAO and SR+CCAO animals showed severe neurological dysfunction, respectively. A significant association between a high frequency of memory impairments with the group SR+CCAO was observed. The neuropathological alterations in CA1 region of hippocampus were similar among the groups. SR potentiates the negative effects of cerebral hypoperfusion conditions, suggesting that SR could be a factor associated with a worse prognosis after a cerebrovascular event.
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Lenise Jihe
authors.initials: LJ
authors.lastname: Kim
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil; Departamento de Neurologia e Neurocirurgia, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address: fernandomorgadinho@hotmail.com.
authors.forename: Fernando Morgadinho
authors.initials: FM
authors.lastname: Coelho
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Araujo
authors.affiliation: Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Roberto Carlos
authors.initials: RC
authors.lastname: Tedesco
authors.affiliation: Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Rodrigo Barbosa
authors.initials: RB
authors.lastname: Souza
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Tufik
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Monica Levy
authors.initials: ML
authors.lastname: Andersen
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D056547:CA1 Region, Hippocampal / Q000473:pathology; D002540:Cerebral Cortex / Q000098:blood supply* / Q000503:physiopathology*; D003345:Corticosterone / Q000097:blood; D004195:Disease Models, Animal; D008297:Male; D008569:Memory Disorders / Q000150:complications / Q000503:physiopathology*; D057567:Memory, Long-Term / Q000502:physiology; D008570:Memory, Short-Term / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D016019:Survival Analysis
chemical_list: D003345:Corticosterone
keywords: Cerebral hypoperfusion; Memory; Mortality rate; Neurological function; Sleep restriction