title: Differential expression profiles and pathways of genes in sugarcane leaf at elongation stage in response to drought stress.
abstract: Water stress causes considerable yield losses in sugarcane. To investigate differentially expressed genes under water stress, a pot experiment was performed with the sugarcane variety GT21 at three water-deficit levels (mild, moderate, and severe) during the elongation stage and gene expression was analyzed using microarray technology. Physiological parameters of sugarcane showed significant alterations in response to drought stress. Based on the expression profile of 15,593 sugarcane genes, 1,501 (9.6%) genes were differentially expressed under different water-level treatments; 821 genes were upregulated and 680 genes were downregulated. A gene similarity analysis showed that approximately 62.6% of the differentially expressed genes shared homology with functional proteins. In a Gene Ontology (GO) analysis, 901 differentially expressed genes were assigned to 36 GO categories. Moreover, 325 differentially expressed genes were classified into 101 pathway categories involved in various processes, such as the biosynthesis of secondary metabolites, ribosomes, carbon metabolism, etc. In addition, some unannotated genes were detected; these may provide a basis for studies of water-deficit tolerance. The reliability of the observed expression patterns was confirmed by RT-PCR. The results of this study may help identify useful genes for improving drought tolerance in sugarcane.
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Changning
authors.initials: C
authors.lastname: Li
authors.affiliation: Microbiology Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Nong
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Solanki
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Jinlan
authors.initials: J
authors.lastname: Xie
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Weizan
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Agriculture, State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi 530004, China.
authors.forename: Litao
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China.
authors.forename: Yangrui
authors.initials: Y
authors.lastname: Li
mesh_terms: D055864:Droughts; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031786:Saccharum / Q000235:genetics* / Q000254:growth & development; D015398:Signal Transduction / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics; D014867:Water / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014867:Water