title: Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses.
abstract: Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Waggoner
authors.forename: Lionel
authors.initials: L
authors.lastname: Gresh
authors.forename: Alisha
authors.initials: A
authors.lastname: Mohamed-Hadley
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ballesteros
authors.forename: Maria Jose Vargas
authors.initials: MJ
authors.lastname: Davila
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Tellez
authors.forename: Malaya K
authors.initials: MK
authors.lastname: Sahoo
authors.forename: Angel
authors.initials: A
authors.lastname: Balmaseda
authors.forename: Eva
authors.initials: E
authors.lastname: Harris
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Pinsky
mesh_terms: D002646:Chikungunya virus / Q000302:isolation & purification*; D003716:Dengue Virus / Q000302:isolation & purification*; D016679:Genome, Viral; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D000071244:Zika Virus / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral
keywords: Zika virus; arboviruses; chikungunya virus; dengue virus; multiplex molecular diagnostics; real-time RT-PCR; vector-borne infections; viruses