title: The complete nucleotide sequence and genomic characterization of tropical soda apple mosaic virus.
abstract: We report the first complete genome sequence of tropical soda apple mosaic virus (TSAMV), a tobamovirus originally isolated from tropical soda apple (Solanum viarum) collected in Okeechobee, Florida. The complete genome of TSAMV is 6,350 nucleotides long and contains four open reading frames encoding the following proteins: i) 126-kDa methyltransferase/helicase (3354 nt), ii) 183-kDa polymerase (4839 nt), iii) movement protein (771 nt) and iv) coat protein (483 nt). The complete genome sequence of TSAMV shares 80.4 % nucleotide sequence identity with pepper mild mottle virus (PMMoV) and 71.2-74.2 % identity with other tobamoviruses naturally infecting members of the Solanaceae plant family. Phylogenetic analysis of the deduced amino acid sequences of the 126-kDa and 183-kDa proteins and the complete genome sequence place TSAMV in a subcluster with PMMoV within the Solanaceae-infecting subgroup of tobamoviruses.
authors.affiliation: Biology Department, Indian River State College, 3209 Virginia Ave., Fort Pierce, FL, 34981, USA.
authors.forename: Kornelia
authors.initials: K
authors.lastname: Fillmer
authors.affiliation: USDA, ARS, US Horticultural Research Laboratory, 2001 South Rock Rd, Fort Pierce, FL, 34945, USA. scott.adkins@ars.usda.gov.
authors.forename: Scott
authors.initials: S
authors.lastname: Adkins
authors.affiliation: Biology Department, Indian River State College, 3209 Virginia Ave., Fort Pierce, FL, 34981, USA.
authors.forename: Patchara
authors.initials: P
authors.lastname: Pongam
authors.affiliation: Biology Department, Indian River State College, 3209 Virginia Ave., Fort Pierce, FL, 34981, USA. tdelia@irsc.edu.
authors.forename: Tom
authors.initials: T
authors.lastname: D'Elia
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D023281:Genomics; D027845:Malus / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017898:Tobamovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins