title: Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.
abstract: Catenation links between sister chromatids are formed progressively during DNA replication and are involved in the establishment of sister chromatid cohesion. Topo IV is a bacterial type II topoisomerase involved in the removal of catenation links both behind replication forks and after replication during the final separation of sister chromosomes. We have investigated the global DNA-binding and catalytic activity of Topo IV in E. coli using genomic and molecular biology approaches. ChIP-seq revealed that Topo IV interaction with the E. coli chromosome is controlled by DNA replication. During replication, Topo IV has access to most of the genome but only selects a few hundred specific sites for its activity. Local chromatin and gene expression context influence site selection. Moreover strong DNA-binding and catalytic activities are found at the chromosome dimer resolution site, dif, located opposite the origin of replication. We reveal a physical and functional interaction between Topo IV and the XerCD recombinases acting at the dif site. This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle.
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR-CNRS 7241, Paris, France.|Université Paris-Saclay, Gif-sur-Yvette, France.
authors.forename: Hafez
authors.identifier: 0000-0002-0969-3785
authors.initials: H
authors.lastname: El Sayyed
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR-CNRS 7241, Paris, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Le Chat
authors.affiliation: Laboratoire de Microbiologie et de Génétique Moléculaires (LMGM), CNRS-Université Toulouse III, Toulouse, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Lebailly
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR-CNRS 7241, Paris, France.|Université Paris-Saclay, Gif-sur-Yvette, France.
authors.forename: Elise
authors.identifier: 0000-0001-8537-5183
authors.initials: E
authors.lastname: Vickridge
authors.affiliation: Laboratoire de Microbiologie et de Génétique Moléculaires (LMGM), CNRS-Université Toulouse III, Toulouse, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Pages
authors.affiliation: Laboratoire de Microbiologie et de Génétique Moléculaires (LMGM), CNRS-Université Toulouse III, Toulouse, France.
authors.forename: Francois
authors.initials: F
authors.lastname: Cornet
authors.affiliation: UMR 7238, Computational and quantitative biology, Institut de biologie Paris Seine, Paris, France.
authors.forename: Marco
authors.initials: M
authors.lastname: Cosentino Lagomarsino
authors.affiliation: Center for Interdisciplinary Research in Biology (CIRB), Collège de France, UMR-CNRS 7241, Paris, France.
authors.forename: Olivier
authors.identifier: 0000-0003-2804-2192
authors.initials: O
authors.lastname: Espéli
mesh_terms: D001665:Binding Sites / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002842:Chromatids / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D012854:Sister Chromatid Exchange / Q000235:genetics
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D029968:Escherichia coli Proteins; C533037:MatP protein, E coli; C473360:XerC protein, E coli; D019426:Integrases; C473361:XerD protein, E coli; D027101:DNA Topoisomerase IV