title: Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein.
abstract: Chikungunya virus (CHIKV), a tropical pathogen, has re-emerged and has massive outbreaks abruptly all over the world. Containing many dominant epitopes, the envelope E2 protein of CHIKV has been explored for the vaccination or diagnosis. In the present study, the antigenicity of a recombinant expressed intrinsically disorder domain (IUD) of E2 was tested for the detection of the antibody against CHIKV through western blot method. The gene of the IUD of E2 was inserted into 2 different vectors and expressed as recombinant GST-E2 and recombinant MBP-E2 fusion protein, respectively. Two kinds of fusion proteins were tested with 30 CHIKV patient sera and 30 normal sera, respectively. Both proteins were detected by 25 patients sera (83.3%) and 1 normal serum (3.3%). This test showed a relatively high sensitivity and very high specificity of the recombinant E2 proteins to be used as diagnostic antigens against CHIKV infection.
authors.affiliation: Department of Parasitology, College of Medicine, Catholic University of Korea, Seoul 06591, Korea.
authors.forename: Zhaoshou
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Biochemistry, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Jihoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Parasitology, College of Medicine, Catholic University of Korea, Seoul 06591, Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Ahn
authors.affiliation: Genbody Inc., Cheonan 31116, Korea.
authors.forename: Chom-Kyu
authors.initials: CK
authors.lastname: Chong
authors.affiliation: Bahia Farma Diagnostics, Cep 43.700-000, Simoes, Bahia, Brazil.
authors.forename: Ronaldo F
authors.initials: RF
authors.lastname: Dias
authors.affiliation: Department of Parasitology, College of Medicine, Catholic University of Korea, Seoul 06591, Korea.
authors.forename: Ho-Woo
authors.initials: HW
authors.lastname: Nam
mesh_terms: D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology*; D065632:Chikungunya Fever / Q000175:diagnosis* / Q000821:virology; D002646:Chikungunya virus / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D058619:Maltose-Binding Proteins / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D005982:Glutathione Transferase
keywords: Chikungunya virus (CHIKV); GST-E2; MBP-E2; envelope E2 protein; intrinsically unstructured domain (IUD); patient sera; western blot