title: Methionine-choline deprivation alters liver and brain acetylcholinesterase activity in C57BL6 mice.
abstract: Choline and methionine are precursors of acetylcholine, whose hydrolysis is catalyzed by acetylcholinesterase (AChE). Considering the possibility of their common deficiency, we investigated the influence of methionine-choline deprivation on AChE activity in liver and various brain regions (hypothalamus, hippocampus, cerebral cortex and striatum) in mice fed with methionine-choline deficient (MCD) diet. Male C57BL/6 mice (n = 28) were randomly and equally divided into following groups: control group fed with standard diet for 6 weeks (C) and groups fed with MCD diet for 2 weeks (MCD2), 4 weeks (MCD4) and for 6 weeks (MCD6). After the diet, mice were sacrificied and AChE activity in liver and brain was determined spectrophotometrically. Hepatic AChE activity was higher in MCD2, MCD4 and MCD6 compared to control (p < 0.01), with most prominent increase in MCD6. AChE activity in hypothalamus was higher in MCD4 and MCD6 vs. control (p < 0.05 and p < 0.01, respectively), as well as in MCD6 compared to MCD4 (p < 0.01). In hippocampus, increase in AChE activity was shown in MCD6 compared to control (p < 0.01). In cortex and striatum, increase in AChE activity was noted in MCD6 compared to control (p < 0.05). Our findings indicate the increase of hepatic and brain AChE activity in mice caused by methionine-choline deprivation.
authors.affiliation: Institute of Pathophysiology "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Dr Subotića 9, 11000 Belgrade, Serbia. danibovuc@med.bg.ac.rs.
authors.forename: Danijela B
authors.initials: DB
authors.lastname: Vučević
authors.forename: Ivana B
authors.initials: IB
authors.lastname: Cerović
authors.forename: Dušan R
authors.initials: DR
authors.lastname: Mladenović
authors.forename: Milena N
authors.initials: MN
authors.lastname: Vesković
authors.forename: Ivana
authors.initials: I
authors.lastname: Stevanović
authors.forename: Bojan Z
authors.initials: BZ
authors.lastname: Jorgačević
authors.forename: Rada
authors.initials: R
authors.lastname: Ješić Vukićević
authors.forename: Tatjana S
authors.initials: TS
authors.lastname: Radosavljević
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002796:Choline Deficiency / Q000378:metabolism*; D004789:Enzyme Activation; D008099:Liver / Q000201:enzymology*; D008297:Male; D008715:Methionine / Q000172:deficiency*; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D008715:Methionine; D000110:Acetylcholinesterase