title: Distribution analysis of profilin isoforms at transcript resolution with mRNA-seq and secondary structure in various organs of Rattus norvegicus.
abstract: Profilin (Pfn) is an actin binding protein, ubiquitously found in mammals and is essential for the actin polymerization in cells. In brain, it plays a pivotal role in neurogenesis and synapse formation by interacting with various proteins. Four Pfn isoforms have been identified in mammals. This study presents the identification and transcriptional expression of various Pfn isoforms (Pfn1, Pfn2, Pfn3 and Pfn4) in brain, heart, kidney, liver, and muscle and testis of Rattus norvegicus. Organs have been classified into groups based on some similarities. Group I includes brain and testis, Group II includes skeletal muscle and heart, while Group III includes kidney and liver. Pfn1 has been identified in all groups, Pfn2 and Pfn3 have been identified in group I, group III and in one organ (skeletal muscle) of group II. To the best of the authors knowledge, no report of Pfn1 and Pfn2 presence in testis, Pfn3 in brain, liver and skeletal muscle, Pfn4 in kidney and skeletal muscle exists to date. Transcriptional expression showed variations among expression level of different Pfn isoforms in various organs with respect to the control gene GADPH. We hypothesize that this could be attributed to profilin isoform specific mRNA structure and corresponding motifs, which generally contribute to similar or varied decay rates, cellular localization, post transcriptional regulation pattern and ligand binding.
authors.affiliation: Department of Biological sciences, Forman Christian College (A Chartered University), 54600 Lahore, Pakistan.
authors.forename: Naila
authors.initials: N
authors.lastname: Tariq
authors.affiliation: Department of Environmental Sciences, Fatima Jinnah Women University, 46000 Rawalpindi, Pakistan.
authors.forename: Zarrin
authors.initials: Z
authors.lastname: Basharat
authors.affiliation: Department of Biological sciences, Forman Christian College (A Chartered University), 54600 Lahore, Pakistan.
authors.forename: Saba
authors.initials: S
authors.lastname: Butt
authors.affiliation: Department of Biological sciences, Forman Christian College (A Chartered University), 54600 Lahore, Pakistan.
authors.forename: Deeba Noreen
authors.initials: DN
authors.lastname: Baig
mesh_terms: D000199:Actins / Q000235:genetics* / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D005786:Gene Expression Regulation; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D010179:Pancreas / Q000737:chemistry / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D051304:Profilins / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D013737:Testis / Q000737:chemistry / Q000378:metabolism; D014158:Transcription, Genetic; D054460:rho-Associated Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D051304:Profilins; D020033:Protein Isoforms; D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C578418:ROCK1 protein, rat; D054460:rho-Associated Kinases
keywords: Profilin; RNA motif; Rattus norvegicus; Sequence analysis; Transcript analysis