title: The Corneal Micropocket Assay: A Model of Angiogenesis and Lymphangiogenesis.
abstract: The cornea is a transparent tissue that lacks blood and lymphatic vessels. In addition, the cornea is readily accessible, which makes it convenient for direct visualization of angiogenesis and lymphangiogenesis. The corneal micropocket assay is a commonly used quantitative technique, in which a growth factor containing pellet is micro-surgically implanted into the cornea of a rodent. Subsequently, the growth of the preexisting limbal vessels toward the growth factor is visualized by live microscopy or immunohistochemistry. Recently, there has been significant interest in the process of lymphangiogenesis and the factors that regulate it. To facilitate these studies, we introduce a novel technique for visualization of the immune response during growth factor induced angiogenesis and lymphangiogenesis in the cornea.
authors.affiliation: Center for Excellence in Functional and Molecular Imaging, Brigham and Women's Hospital, 75 Francis Street, Boston, MA, 02115, USA.|Department of Radiology, Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Nakao
authors.affiliation: Center for Excellence in Functional and Molecular Imaging, Brigham and Women's Hospital, 75 Francis Street, Boston, MA, 02115, USA. ahm@bwh.harvard.edu.|Department of Radiology, Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA. ahm@bwh.harvard.edu.
authors.forename: Ali
authors.initials: A
authors.lastname: Hafezi-Moghadam
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003315:Cornea / Q000098:blood supply / Q000187:drug effects* / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000494:pharmacology; D053583:Interleukin-1beta / Q000378:metabolism; D042583:Lymphangiogenesis; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D020556:Neutrophil Infiltration
chemical_list: D015815:Cell Adhesion Molecules; D036341:Intercellular Signaling Peptides and Proteins; D053583:Interleukin-1beta; D006631:Amine Oxidase (Copper-Containing); C492678:semicarbazide-sensitive amine oxidase-vascular adhesion protein-1, mouse
keywords: Conjunctiva; Cornea; Growth factor; Imaging; LYVE-1; Leukocyte recruitment; Limbal vessel; Lymphangiogenesis; VEGF