title: Investigation of Migration and Differentiation of Human Mesenchymal Stem Cells on Five-Layered Collagenous Electrospun Scaffold Mimicking Native Cartilage Structure.
abstract: Cartilage degeneration is the major cause of chronic pain, lost mobility, and reduced quality of life for over estimated 150 million osteoarthritis sufferers worldwide. Despite intensive research, none of the available therapies can restore the hyaline cartilage surface beyond just fibrous repair. To overcome these limitations, numerous cell-based approaches for cartilage repair are being explored that aim to provide an appropriate microenvironment for chondrocyte maintenance and differentiation of multipotent mesenchymal stem cells (MSCs) toward the chondrogenic lineage. Articular cartilage is composed of highly organized collagen network that entails the tissue into four distinct zones and each zone into three different regions based on differences in matrix morphology and biochemistry. Current cartilage implants cannot establish the hierarchical tissue organization that seems critical for normal cartilage function. Therefore, in this study, a structured, multilayered collagen scaffold designed for the replacement of damaged cartilage is presented that allows repopulation by host cells and synthesis of a new natural matrix. By using the electrospinning method, the potential to engineer a scaffold consisting of two different collagen types is obtained. With the developed collagen scaffold, a five-layered biomaterial is created that has the potency to induce the differentiation of human bone marrow derived MSCs toward the chondrogenic lineage.
authors.affiliation: Department Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070, Würzburg, Germany. jenny.reboredo@uni-wuerzburg.de.
authors.forename: Jenny W
authors.initials: JW
authors.lastname: Reboredo
authors.affiliation: Department Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070, Würzburg, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Weigel
authors.affiliation: Department of Orthopedic Surgery, König-Ludwig-Haus Orthopaedic Center for Musculoskeletal Research, Julius-Maximilians-University Würzburg, Brettreichstraße 11, Würzburg, 97074, Germany.
authors.forename: Andre
authors.initials: A
authors.lastname: Steinert
authors.affiliation: Department of Orthopedic Surgery, König-Ludwig-Haus Orthopaedic Center for Musculoskeletal Research, Julius-Maximilians-University Würzburg, Brettreichstraße 11, Würzburg, 97074, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Rackwitz
authors.affiliation: Department Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070, Würzburg, Germany.|Department of Orthopedic Surgery, König-Ludwig-Haus Orthopaedic Center for Musculoskeletal Research, Julius-Maximilians-University Würzburg, Brettreichstraße 11, Würzburg, 97074, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Rudert
authors.affiliation: Department Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070, Würzburg, Germany.|Translational Center Würzburg "Regenerative Therapies in Oncology and Musculoskeletal Diseases" Würzburg Branch, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Röntgenring 11, 97070, Würzburg, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Walles
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D002465:Cell Movement; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: biomaterials; cartilage tissue engineering; collagen structure; electrospinning; mesenchymal stem cells