title: Lentivirus-mediated knockdown of M-phase phosphoprotein 8 inhibits proliferation of colon cancer cells.
abstract: M-phase phosphoprotein 8 (MPP8) has been reported to be overexpressed in various human carcinoma cells and was associated with tumor malignant characters. However, its functional role in colon cancer (CRC) is still unclear. In the present study, lentivirus-mediated short hairpin RNAs were designed to silence the MPP8 gene in CRC cells including RKO and SW1116 cells. The fluorescence microscopy was used to determine the knockdown efficiency of MPP8 by observing lentivirus-mediated green fluorescent protein expression. MPP8 expression in infected RKO and SW1116 was evaluated by real-time PCR and Western blot analysis. Cell proliferation was assessed by MTT assay and colony formation. Flow cytometry was applied to measure cell cycle and apoptosis. Transwell assay was used to determine the effect of MMP8 silencing on cell migration. Our results demonstrated that loss of MPP8 inhibited cell proliferation and migration and promoted cell apoptosis. These results indicate that MPP8 plays an important role in the proliferation and metastasis of CRC cells and suggest that silencing of MPP8 may be an effective therapeutic approach for the treatment of CRC.
authors.affiliation: Department of Gastroenterology, Taizhou Central Hospital, Taizhou, People's Republic of China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Colorectal, Second Affiliated Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Tongjun
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Hand & Foot Surgery, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Colorectal, Second Affiliated Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Taizhou Central Hospital, Taizhou, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, Taizhou Central Hospital, Taizhou, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000378:metabolism*; D010750:Phosphoproteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: C547278:MPHOSPH8 protein, human; D010750:Phosphoproteins
keywords: M-phase phosphoprotein 8 (MPP8); cell proliferation; colon cancer