title: Lateral Thalamic Eminence: A Novel Origin for mGluR1/Lot Cells.
abstract: A unique population of cells, called "lot cells," circumscribes the path of the lateral olfactory tract (LOT) in the rodent brain and acts to restrict its position at the lateral margin of the telencephalon. Lot cells were believed to originate in the dorsal pallium (DP). We show that Lhx2 null mice that lack a DP show a significant increase in the number of mGluR1/lot cells in the piriform cortex, indicating a non-DP origin of these cells. Since lot cells present common developmental features with Cajal-Retzius (CR) cells, we analyzed Wnt3a- and Dbx1-reporter mouse lines and found that mGluR1/lot cells are not generated in the cortical hem, ventral pallium, or septum, the best characterized sources of CR cells. Finally, we identified a novel origin for the lot cells by combining in utero electroporation assays and histochemical characterization. We show that mGluR1/lot cells are specifically generated in the lateral thalamic eminence and that they express mitral cell markers, although a minority of them express ΔNp73 instead. We conclude that most mGluR1/lot cells are prospective mitral cells migrating to the accessory olfactory bulb (OB), whereas mGluR1+, ΔNp73+ cells are CR cells that migrate through the LOT to the piriform cortex and the OB.
authors.affiliation: Instituto de Neurociencias (Consejo Superior de Investigaciones Científicas - Universidad Miguel Hernández, CSIC - UMH), San Juan de Alicante, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Ruiz-Reig
authors.affiliation: Instituto de Neurociencias (Consejo Superior de Investigaciones Científicas - Universidad Miguel Hernández, CSIC - UMH), San Juan de Alicante, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Andrés
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.|Current address: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Huilgol
authors.affiliation: Department of Neurobiology, University of Chicago, Chicago, IL, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Grove
authors.affiliation: Université catholique de Louvain, Institute of Neuroscience, Brussels, Belgium.
authors.forename: Fadel
authors.initials: F
authors.lastname: Tissir
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.
authors.forename: Shubha
authors.initials: S
authors.lastname: Tole
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK.
authors.forename: Thomas
authors.initials: T
authors.lastname: Theil
authors.affiliation: Instituto de Neurociencias (Consejo Superior de Investigaciones Científicas - Universidad Miguel Hernández, CSIC - UMH), San Juan de Alicante, Spain.
authors.forename: Eloisa
authors.initials: E
authors.lastname: Herrera
authors.affiliation: Instituto de Neurociencias (Consejo Superior de Investigaciones Científicas - Universidad Miguel Hernández, CSIC - UMH), San Juan de Alicante, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Fairén
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D011247:Pregnancy; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000378:metabolism; D013788:Thalamus / Q000166:cytology* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000072160:Tumor Protein p73 / Q000235:genetics / Q000378:metabolism
chemical_list: C103403:Dbx1 protein, mouse; C491198:Eomes protein, mouse; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C489784:Lhx2 protein, mouse; D009419:Nerve Tissue Proteins; D018094:Receptors, Metabotropic Glutamate; D020825:T-Box Domain Proteins; D014157:Transcription Factors; C000606368:Trp73 protein, mouse; D000072160:Tumor Protein p73; C104077:metabotropic glutamate receptor type 1
keywords: Cajal–Retzius cells; Tbr2; accessory olfactory bulb; lateral thalamic eminence; lot cells