title: Variation in fungal microbiome (mycobiome) and aflatoxins during simulated storage of in-shell peanuts and peanut kernels.
abstract: Internal transcribed spacer 2 (ITS2) sequencing was used to characterize the peanut mycobiome during 90 days storage at five conditions. The fungal diversity in in-shell peanuts was higher with 110 operational taxonomic units (OTUs) and 41 genera than peanut kernels (91 OTUs and 37 genera). This means that the micro-environment in shell is more suitable for maintaining fungal diversity. At 20-30 d, Rhizopus, Eurotium and Wallemia were predominant in in-shell peanuts. In peanut kernels, Rhizopus (>30%) and Eurotium (>20%) were predominant at 10-20 d and 30 d, respectively. The relative abundances of Rhizopus, Eurotium and Wallemia were higher than Aspergillus, because they were xerophilic and grew well on substrates with low water activity (aw). During growth, they released metabolic water, thereby favoring the growth of Aspergillus. Therefore, from 30 to 90 d, the relative abundance of Aspergillus increased while that of Rhizopus, Eurotium and Wallemia decreased. Principal Coordinate Analysis (PCoA) revealed that peanuts stored for 60-90 days and for 10-30 days clustered differently from each other. Due to low aw values (0.34-0.72) and low levels of A. flavus, nine of 51 samples were contaminated with aflatoxins.
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Fuguo
authors.initials: F
authors.lastname: Xing
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ding
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Jonathan Nimal
authors.initials: JN
authors.lastname: Selvaraj
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Yueju
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000348:Aflatoxins / Q000378:metabolism*; D010367:Arachis / Q000382:microbiology*; D015169:Colony Count, Microbial; D021901:DNA, Intergenic; D005516:Food Microbiology; D061353:Food Storage / Q000379:methods*; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000072761:Mycobiome; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D000348:Aflatoxins; D021901:DNA, Intergenic