title: Photonic reagents for concentration measurement of flu-orescent proteins with overlapping spectra.
abstract: By exploiting photonic reagents (i.e., coherent control by shaped laser pulses), we employ Optimal Dynamic Discrimination (ODD) as a novel means for quantitatively characterizing mixtures of fluorescent proteins with a large spectral overlap. To illustrate ODD, we simultaneously measured concentrations of in vitro mixtures of Enhanced Blue Fluorescent Protein (EBFP) and Enhanced Cyan Fluorescent Protein (ECFP). Building on this foundational study, the ultimate goal is to exploit the capabilities of ODD for parallel monitoring of genetic and protein circuits by suppressing the spectral cross-talk among multiple fluorescent reporters.
authors.affiliation: Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Alexei
authors.initials: A
authors.lastname: Goun
authors.affiliation: Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Denys I
authors.initials: DI
authors.lastname: Bondar
authors.affiliation: Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Ali O
authors.initials: AO
authors.lastname: Er
authors.affiliation: Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Quine
authors.affiliation: Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Herschel A
authors.initials: HA
authors.lastname: Rabitz
mesh_terms: D000465:Algorithms; D002457:Cell Extracts; D004926:Escherichia coli / Q000378:metabolism; D007202:Indicators and Reagents; D007834:Lasers; D008164:Luminescent Proteins / Q000378:metabolism*; D017785:Photons; D013050:Spectrometry, Fluorescence
chemical_list: D002457:Cell Extracts; D007202:Indicators and Reagents; D008164:Luminescent Proteins