title: Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Midgut Response to BmNPV in Susceptible and Near-Isogenic Resistant Strains.
abstract: Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens causing severe economic losses in sericulture. However, the molecular mechanism of silkworm resistance to BmNPV remains largely unknown. Here, the recurrent parent P50 (susceptible strain) and the near-isogenic line BC9 (resistance strain) were used in a comparative transcriptome study examining the response to infection with BmNPV. A total of 14,300 unigenes were obtained from two different resistant strains; of these, 869 differentially expressed genes (DEGs) were identified after comparing the four transcriptomes. Many DEGs associated with protein metabolism, cytoskeleton, and apoptosis may be involved in the host response to BmNPV infection. Moreover, some immunity related genes were also altered following BmNPV infection. Specifically, after removing genetic background and individual immune stress response genes, 22 genes were found to be potentially involved in repressing BmNPV infection. These genes were related to transport, virus replication, intracellular innate immune, and apoptosis. Our study provided an overview of the molecular mechanism of silkworm resistance to BmNPV infection and laid a foundation for controlling BmNPV in the future.
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Xue-Yang
authors.initials: XY
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Hai-Zhong
authors.initials: HZ
authors.lastname: Yu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Geng
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Jia-Ping
authors.initials: JP
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yu
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Shang-Zhi
authors.initials: SZ
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, People's Republic of China.
authors.forename: Dong-Qiong
authors.initials: DQ
authors.lastname: Fei
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000276:immunology; D012831:Bombyx / Q000235:genetics* / Q000276:immunology / Q000821:virology; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics* / Q000276:immunology; D007814:Larva / Q000235:genetics* / Q000276:immunology / Q000821:virology; D058977:Molecular Sequence Annotation; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity / Q000502:physiology*; D059467:Transcriptome / Q000276:immunology*; D014779:Virus Replication / Q000276:immunology
chemical_list: D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D019476:Insect Proteins