title: Naked-eye fingerprinting of single nucleotide polymorphisms on psoriasis patients.
abstract: We report a low-cost test, based on gold nanoparticles, for the colorimetric (naked-eye) fingerprinting of a panel of single nucleotide polymorphisms (SNPs), relevant for the personalized therapy of psoriasis. Such pharmacogenomic tests are not routinely performed on psoriasis patients, due to the high cost of standard technologies. We demonstrated high sensitivity and specificity of our colorimetric test by validating it on a cohort of 30 patients, through a double-blind comparison with two state-of-the-art instrumental techniques, namely reverse dot blotting and sequencing, finding 100% agreement. This test offers high parallelization capabilities and can be easily generalized to other SNPs of clinical relevance, finding broad utility in diagnostics and pharmacogenomics.
authors.affiliation: Nanobiointeractions & Nanodiagnostics, Istituto Italiano di Tecnologia (IIT), Via Morego, 30-16163 - Genova, Italy. pierpaolo.pompa@iit.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Valentini
authors.forename: Alessandra
authors.initials: A
authors.lastname: Marsella
authors.forename: Paolo
authors.initials: P
authors.lastname: Tarantino
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mauro
authors.forename: Silvia
authors.initials: S
authors.lastname: Baglietto
authors.forename: Maurizio
authors.initials: M
authors.lastname: Congedo
authors.forename: Pier
authors.initials: P
authors.lastname: Paolo Pompa
mesh_terms: D003124:Colorimetry; D016172:DNA Fingerprinting / Q000379:methods*; D004311:Double-Blind Method; D005260:Female; D006046:Gold; D015236:HLA-C Antigens / Q000235:genetics; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011565:Psoriasis / Q000235:genetics*; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3 / Q000235:genetics
chemical_list: D015236:HLA-C Antigens; C491620:HLA-C*06:02 antigen; D006046:Gold; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3