title: Characteristics of peripheral blood CD4+CD25+ regulatory T cells and related cytokines in severe atopic dermatitis.
abstract: Regulatory T cells (Tregs) have been suggested to play a role in the pathogenesis of atopic dermatitis (AD). However, alterations in the ability of Tregs remain to be determined. To investigate the expression of various surface receptors on CD4(+)CD25(high) regulatory T cells and to investigate their capacity for inhibiting the proliferation of CD4(+) CD25(-) effector T cells (Teffs). Peripheral blood samples were obtained from 15 patients with severe atopic dermatitis (AD) and 20 control subjects. FACs was then carried out to analyze the expression levels of FoxP3, CD152 (CTLA-4), CD39, CD73, CD223 (LAG-3), CCR4, CCR5, and CCR10 on Tregs. The proliferative responses of Teffs were assessed in the absence or presence of autologous Tregs and the TGF-β1 and IL-10 levels in the culture supernatant and sera were detected by enzyme-linked immunosorbent assay (ELISA). The CD152, CD39, CD73, CCR4, and CCR5 expression levels on Tregs were higher in patients with severe AD than in the controls. Tregs showed an attenuated suppressive function of the proliferation of autologous Teffs in severe AD. The concentrations of IL-10 and TGF-β in the culture supernatants of Tregs were lower in the AD group than in the control. The attenuated ability of Tregs to suppress Teff proliferation may be responsible for the autoimmune reaction of severe AD.
authors.affiliation: Department of Dermatology.
authors.forename: Yun-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Dermatology.
authors.forename: Ao-Xue
authors.initials: AX
authors.lastname: Wang
authors.affiliation: Department of Dermatology.
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Dermatology.
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.affiliation: Department of Clinical Laboratory, the 2nd Affiliated Hospital of Dalian Medical University No.467 Zhongshan Road Dalian, 116027, Liaoning Province P R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Dermatology.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Tu
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D001081:Apyrase / Q000032:analysis; D060908:CTLA-4 Antigen / Q000032:analysis; D016022:Case-Control Studies; D049109:Cell Proliferation; D002478:Cells, Cultured; D003876:Dermatitis, Atopic / Q000097:blood*; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis; D058851:GPI-Linked Proteins / Q000032:analysis; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000378:metabolism*; D008297:Male; D054447:Receptors, CCR10 / Q000032:analysis; D054398:Receptors, CCR4 / Q000032:analysis; D019713:Receptors, CCR5 / Q000032:analysis; D012720:Severity of Illness Index; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry* / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000378:metabolism*; D000093322:Lymphocyte Activation Gene 3 Protein
chemical_list: D015703:Antigens, CD; C515706:CCR10 protein, human; C515354:CCR4 protein, human; C584933:CCR5 protein, human; D060908:CTLA-4 Antigen; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D058851:GPI-Linked Proteins; D054447:Receptors, CCR10; D054398:Receptors, CCR4; D019713:Receptors, CCR5; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D015720:5'-Nucleotidase; C548241:NT5E protein, human; D001081:Apyrase; C067869:CD39 antigen; D000093322:Lymphocyte Activation Gene 3 Protein; C000720326:Lag3 protein, human
keywords: atopic dermatitis; effector T cells; regulatory T cells