title: Prediction of the impact of coding missense and nonsense single nucleotide polymorphisms on HD5 and HBD1 antibacterial activity against Escherichia coli.
abstract: Defensins confer host defense against microorganisms and are important for human health. Single nucleotide polymorphisms (SNPs) in defensin gene-coding regions could lead to less active variants. Using SNP data available at the dbSNP database and frequency information from the 1000 Genomes Project, two DEFA5 (L26I and R13H) and eight DEFB1 (C35S, K31T, K33R, R29G, V06I, C12Y, Y28* and C05*) missense and nonsense SNPs that are located within mature regions of the coded defensins were retrieved. Such SNPs are rare and population restricted. In order to assess their antibacterial activity against Escherichia coli, two linear regression models were used from a previous work, which models the antibacterial activity as a function of solvation potential energy, using molecular dynamics data. Regarding only the antibacterial predictions, for HD5, no biological differences between wild-type and its variants were observed; while for HBD1, the results suggest that the R29G, K31T, Y28* and C05* variants could be less active than the wild-type one. The data here reported could lead to a substantial improvement in knowledge about the impact of missense SNPs in human defensins and their world distribution. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 633-644, 2016.
authors.affiliation: Programa De Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil.|Centro De Análises Proteômicas E Bioquímicas, Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil.
authors.forename: William F
authors.initials: WF
authors.lastname: Porto
authors.affiliation: Programa De Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil.|Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA.
authors.forename: Diego O
authors.initials: DO
authors.lastname: Nolasco
authors.affiliation: Programa De Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil.|Centro De Análises Proteômicas E Bioquímicas, Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil.
authors.forename: Állan S
authors.initials: ÁS
authors.lastname: Pires
authors.affiliation: Programa De Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil.
authors.forename: Rinaldo W
authors.initials: RW
authors.lastname: Pereira
authors.affiliation: Programa De Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil. ocfranco@gmail.com.|Centro De Análises Proteômicas E Bioquímicas, Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil. ocfranco@gmail.com.|S-Inova Biotech, Pos-Graduação Em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil. ocfranco@gmail.com.
authors.forename: Octávio L
authors.initials: OL
authors.lastname: Franco
authors.affiliation: Programa De Pós-Graduação Em Ciências Genômicas E Biotecnologia, Universidade Católica De Brasília, Brasília, DF, Brazil.
authors.forename: Sérgio A
authors.initials: SA
authors.lastname: Alencar
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects*; D006801:Humans; D056004:Molecular Dynamics Simulation; D020641:Polymorphism, Single Nucleotide; D023084:alpha-Defensins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D023083:beta-Defensins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; C500225:DEFA5 protein, human; C406407:DEFB1 protein, human; D023084:alpha-Defensins; D023083:beta-Defensins
keywords: 1000 genomes project; DEFA5; DEFB1; molecular dynamics; protein solvation potential energy