title: Protective effects of thoracic epidural anesthesia on hypoxia-induced acute lung injury in rabbits.
abstract: The mechanism underlying the effect of thoracic epidural anesthesia (TEA) on hypoxia-induced acute lung injury (ALI) is currently unknown. In the present study, a rabbit acute lung injury model was established to investigate the effects of TEA on inflammatory factors, pulmonary surfactant and ultrastructure. A total of 56 rabbits were randomly assigned to four groups (n=14 per group): Control group (Group C), hypoxia group (Group H), sevoflurane group (Group S) and combined sevoflurane-epidural anesthesia group (Group ES). The ALI model was considered to have been successfully induced when the ratio of arterial oxygen partial pressure to fractional inspired oxygen was <300. The correct placement of a catheter for TEA was confirmed using epidurography. ALI was maintained for 3 h. Arterial blood samples were collected from all groups during spontaneous breathing (T0) and at 3 h after ALI induction (T5) in order to evaluate the serum levels of interleukin (IL)-6, IL-8 and IL-10. Bronchoalveolar lavage fluid was harvested to determine the total phospholipid, saturated phosphatidylcholine and total protein levels. Furthermore, the dry/wet weight ratio and the mRNA expression levels of IL-6, IL-8 and IL-10 in the lung tissue were determined using ELISA. In addition, light and transmission electron microscopy and histological techniques were used to examine the morphology of alveolar type II cells in the rat lung tissue. The results indicate that changes of serum IL-6, IL-8 and IL-10 levels following ALI were consistent with the changes in the mRNA expression levels of IL-6, IL-8 and IL-10 in the lung tissue. TEA attenuated these changes and thus reduced the severity of the ALI. In addition, TEA improved the alveolar structure, reduced the number of polymorphonuclear cells and mitigated the damage of lamellar bodies. In summary, the results of the present study indicate that TEA reduces lung tissue damage by inhibiting systemic and local inflammation, decreasing the inactivation of pulmonary surfactant and improving the alveolar ultrastructure following ALI.
authors.affiliation: Department of Anesthesiology, Ear, Eye, Nose and Throat Hospital, Fudan University, Shanghai 200031, P.R. China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cang
authors.affiliation: Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Zhanggang
authors.initials: Z
authors.lastname: Xue
keywords: anesthesia; epidural; inflammation; pulmonary surfactants; rabbit; respiratory distress syndrome