title: CREATININE DETERMINATION IN URINE BY LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION-TANDEM MASS SPECTROMETRY METHOD.
abstract: Creatinine determination in urine is used to estimate the completeness of the 24-h urine collection, compensation for variable diuresis and as a preliminary step in protein profiling in urine. Despite the fact that a wide range of methods of measuring creatinine level in biofluids has been developed, many of them are adversely affected by interfering substances. A new liquid chromatography-tandem mass spectrometry method for creatinine determination in urine has been developed. Chromatographic separation was performed by applying C18 column and a gradient elution. Analyses were carried out on a triple quadrupole mass spectrometer equipped with an electrospray ion source. The developed method was fully validated according to the international guidelines. The quantification range of the method was 5-1500 ng/mL, which corresponds to 1-300 mg/dL in urine. Limit of detection and quantitation were 2 and 5 ng/mL, respectively. Additionally, the comparison of creatinine determination by newly developed method to the colorimetric method was performed. The method enables the determination of creatinine in urine samples with a minimal sample preparation, excellent sensitivity and prominent selectivity. Since mass spectrometry allows to measure a number of compounds simultaneously, a future perspective would be to incorporate the determination of other clinically important compounds excreted in urine.
authors.forename: Paweł
authors.initials: P
authors.lastname: Dereziński
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Klupczyńska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Sawicki
authors.forename: Zenon J
authors.initials: ZJ
authors.lastname: Kokot
mesh_terms: D015415:Biomarkers / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D003124:Colorimetry; D003404:Creatinine / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D011237:Predictive Value of Tests; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards; D016482:Urinalysis / Q000379:methods* / Q000592:standards
chemical_list: D015415:Biomarkers; D003404:Creatinine