title: Preparation and characterization of oxidized konjac glucomannan/carboxymethyl chitosan/graphene oxide hydrogel.
abstract: Polysaccharide hydrogels have been widely used as biomaterials in biomedical field. In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS). Meanwhile, different amount of graphene oxide (GO) was added as nano-additive. The hydrogels have been characterized by various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM). Through the observation of SEM, the hydrogels' scaffolds present a homogeneous interconnected pore structure after lyophilizing. In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated. The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate. Especially, the compressive strength and modulus increase 144% and 296% respectively as the GO content increase from 0 to 5mg/ml. Moreover, MTT assay was applied to evaluate the biocompatibility of hydrogels. The result indicate that hydrogels with GO show better biocompatibility. Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Yi
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tong
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Ge
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Shengqiong
authors.initials: S
authors.lastname: Zou
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Huigao
authors.initials: H
authors.lastname: Wen
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: niemin@whu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Nie
mesh_terms: D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D019245:Compressive Strength; D006108:Graphite / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D020100:Hydrogels; D008351:Mannans; C022901:(1-6)-alpha-glucomannan; D006108:Graphite; D048271:Chitosan
keywords: Carboxymethyl chitosan; Graphene oxide; Hydrogel; Mechanical properties; Oxidized konjac glucomannan; Wound healing