title: Expression of salicylic acid-related genes in Brassica oleracea var. capitata during Plasmodiophora brassicae infection.
abstract: Brassica oleracea var. capitata (cabbage) is an important vegetable crop in Asian countries such as Korea, China, and Japan. Cabbage production is severely affected by clubroot disease caused by the soil-borne plant pathogen Plasmodiophora brassicae. During clubroot development, methyl salicylate (MeSA) is biosynthesized from salicylic acid (SA) by methyltransferase. In addition, methyl salicylate esterase (MES) plays a major role in the conversion of MeSA back into free SA. The interrelationship between MES and methytransferases during clubroot development has not been fully explored. To begin to examine these relationships, we investigated the expression of MES genes in disease-susceptible and disease-resistant plants during clubroot development. We identified three MES-encoding genes potentially involved in the defense against pathogen attack. We found that SS1 was upregulated in both the leaves and roots of B. oleracea during P. brassicae infection. These results support the conclusion that SA biosynthesis is suppressed during pathogen infection in resistant plants. We also characterized the expression of a B. oleracea BSMT gene, which appears to be involved in glycosylation rather than MeSA biosynthesis. Our results provide insight into the functions and interactions of genes for MES and methyltransferase during infection. Taken together, our findings indicate that MES genes are important candidates for use to control clubroot diseases.
authors.affiliation: Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.|Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.
authors.forename: Ranjith Kumar
authors.initials: RK
authors.lastname: Manoharan
authors.affiliation: Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.|Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.
authors.forename: Ashokraj
authors.initials: A
authors.lastname: Shanmugam
authors.affiliation: Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.|Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.
authors.forename: Indeok
authors.initials: I
authors.lastname: Hwang
authors.affiliation: Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.|Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Park
authors.affiliation: Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.|Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonam 57922, Republic of Korea.
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000469:parasitology*; D018556:Crops, Agricultural / Q000235:genetics / Q000378:metabolism / Q000469:parasitology; D060467:Disease Resistance; D017343:Genes, Plant; D006790:Host-Parasite Interactions; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D056909:Plasmodiophorida / Q000502:physiology*; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism*
chemical_list: D012459:Salicylates; D008780:Methyltransferases; C033069:methyl salicylate; D020156:Salicylic Acid
keywords: acide salicylique; clubroot; hernie du chou; methyl salicylate esterase; methyltransferase; méthyltransférase; salicylate de méthyle estérase; salicylic acid