title: Association of ICAM-1 K469E polymorphism with dengue infection in North Indian population.
abstract: Dengue infection is caused by flavivirus is one of the leading cause of mortality. There are certain factors which play role in the transformation of a mild form of the disease (DF) into a severe form (DHF) but the most important ones are: viral strain virulence, host genetics, and host immune status. In severe dengue infection, plasma leakage occurs due to vascular endothelial cell activation through expression of adhesion molecule like intercellular cell adhesion molecule-1 (ICAM-1). A total of 100 dengue patients (DF; n = 53 and DHF/DSS; n = 47) and 200 healthy controls were included in the study. ICAM-1 K469E genotyping was done by polymerase chain reaction-restriction fragment length polymorphism (PCR- RFLP). Expression of ICAM-1 mRNA was done by Real time reverse transcription- PCR (rRT-PCR). Patients with homozygous genotype (EE) have 3.22 fold risk (P = 0.008) of developing severe form of disease (DHF/DSS) as compared to other genotypes. Patients with DHF/DSS exhibit higher expression of ICAM-1 mRNA as compared to dengue fever and controls (P = 0.001 and < 0.001). Patients (DHF/DSS) with homozygous (EE) genotype exhibit higher expression of ICAM-1 mRNA when compared with wild type (KK) genotype (P = 0.005). This study suggests a possible association between the ICAM-1 polymorphism and the disease severity.
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, U.P 226014, India; Amity Institute of Biotechnology, Amity University, Lucknow, U.P 226028, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, U.P 226014, India.
authors.forename: Satyendra K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, U.P 226014, India; Amity Institute of Biotechnology, Amity University, Lucknow, U.P 226028, India.
authors.forename: Kavita
authors.initials: K
authors.lastname: Kakkar
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, U.P 226014, India.
authors.forename: Nikky
authors.initials: N
authors.lastname: Nyari
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, U.P 226014, India.
authors.forename: Dharamveer
authors.initials: D
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, U.P 226014, India. Electronic address: tndhole@gmail.com.
authors.forename: Tapan N
authors.initials: TN
authors.lastname: Dhole
authors.affiliation: Department of Hematology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, U.P 226014, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kashyap
authors.affiliation: Amity Institute of Biotechnology, Amity University, Lucknow, U.P 226028, India.
authors.forename: Saba
authors.initials: S
authors.lastname: Hasan
mesh_terms: D000328:Adult; D003715:Dengue / Q000235:genetics* / Q000473:pathology*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018570:Risk Assessment; D055815:Young Adult
chemical_list: D018799:Intercellular Adhesion Molecule-1
keywords: Endothelial cells; Genotypes; Intercellular adhesion molecule-1; Severe dengue; Single nucleotide polymorphism