title: The Sponge Implant Model of Angiogenesis.
abstract: The host response observed after the application of an appropriate stimulus, such as mechanical injury or injection of neoplastic or normal tissue implants, has allowed the cataloging of a number of molecules and cells involved in the vascularization of normal repair or neoplastic tissue. Implantation of sponge matrices has been adopted as a model for the accurate quantification of angiogenic and fibrogenic responses, as they may occur during wound healing, in vivo. Such implants are particularly useful because they offer scope for modulating the environment within which angiogenesis occurs. Sponge implantation model has been optimized and adapted to characterize essential components and their roles in blood vessels formation in a variety of physiological and pathological conditions. As a direct consequence of advances in genetic manipulation, mouse models (i.e., knockouts, SCID, nude) have provided resources to delineate the mechanisms regulating the healing associated with implants. Here we outline the usefulness of the sponge implant model of angiogenesis and detailed description of the methodology.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Campus Pampulha, Cx. Post. 486, CEP 31270-901, Belo Horizonte, MG, Brazil. andrades@icb.ufmg.br.
authors.forename: Silvia Passos
authors.initials: SP
authors.lastname: Andrade
authors.affiliation: Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Campus Pampulha, Cx. Post. 486, CEP 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Mônica Alves Neves Diniz
authors.initials: MA
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D051379:Mice; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000473:pathology; D018919:Neovascularization, Physiologic; D011145:Polyvinyls / Q000008:administration & dosage*; D019736:Prostheses and Implants; D051381:Rats
chemical_list: D011145:Polyvinyls; C026699:ivalon sponge
keywords: Angiogenesis; Blood flow; Chemokine; Collagen; Cytokine; Intraperitoneal adhesion; Myeloperoxidase; N-Acetylglucosaminidase; Sponge model