title: Characterization and morphological comparison of human dura mater, temporalis fascia, and pericranium for the correct selection of an autograft in duraplasty procedures.
abstract: OBJECTIVE
  The objective of this study was to characterize and compare the morphological characteristics of the dura mater, the pericranium, and the temporal fascia to ascertain the most adequate tissue to use as a dura graft.
  
  
  METHODS
  20 dura mater, 20 pericranium and 20 temporalis fascia samples were analyzed. Each of the samples was stained with hematoxylin and eosin, orcein, Van Gieson, Masson's trichrome and Verhoeff-Van Gieson (600 slides in total) for a general morphological evaluation, as well as a quantitative, morphometric and densitometric analysis of elastic fibers present in each of the tissues.
  
  
  RESULTS
  The micro-densitometric analysis of the tissues indicated that the area occupied by the elastic fibers showed values of 1.766 ± 1.376, 4.580 ± 3.041, and 8.253 ± 4.467 % for the dura mater, the temporalis fascia and the pericranium, respectively (p < 0.05, all pairs). The values observed in the analysis of the density intensity were 3.42E+06 ± 2.57E+06, 1.41E+07 ± 1.28E+07, and 1.63E+07 ± 9.19E+06 for the dura mater, the temporalis fascia and the pericranium, respectively (p < 0.05), dura mater vs. temporalis fascia and dura mater vs. pericranium).
  
  
  CONCLUSIONS
  This is the first study to compare the dura mater with tissues for dural autograft and to quantify the elastic component present in these tissues. The results indicate that the temporalis fascia is a better dural graft because of its intrinsic tissue properties.
authors.affiliation: Department of Human Anatomy, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Ave. Madero s/n Col. Mitras Centro, C.P.64460, Monterrey, Nuevo León, Mexico. rodolfot59@hotmail.com.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Morales-Avalos
authors.affiliation: Department of Histology, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Monterrey, Nuevo León, Mexico.
authors.forename: Adolfo
authors.initials: A
authors.lastname: Soto-Domínguez
authors.affiliation: Department of Histology, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Monterrey, Nuevo León, Mexico.
authors.forename: Jaime
authors.initials: J
authors.lastname: García-Juárez
authors.affiliation: Department of Histology, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Monterrey, Nuevo León, Mexico.|Centro de Investigación Biomédica del Noreste (CIBIN), IMSS, Monterrey, Nuevo León, Mexico.
authors.forename: Odila
authors.initials: O
authors.lastname: Saucedo-Cardenas
authors.affiliation: Department of Human Anatomy, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Ave. Madero s/n Col. Mitras Centro, C.P.64460, Monterrey, Nuevo León, Mexico.
authors.forename: José R
authors.initials: JR
authors.lastname: Bonilla-Galvan
authors.affiliation: Department of Human Anatomy, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Ave. Madero s/n Col. Mitras Centro, C.P.64460, Monterrey, Nuevo León, Mexico.
authors.forename: Marcela
authors.initials: M
authors.lastname: Cardenas-Serna
authors.affiliation: Department of Human Anatomy, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Ave. Madero s/n Col. Mitras Centro, C.P.64460, Monterrey, Nuevo León, Mexico.
authors.forename: Santos
authors.initials: S
authors.lastname: Guzmán-López
authors.affiliation: Department of Human Anatomy, Faculty of Medicine, Universidad Autonoma de Nuevo León (U.A.N.L.), Ave. Madero s/n Col. Mitras Centro, C.P.64460, Monterrey, Nuevo León, Mexico.
authors.forename: Rodrigo E
authors.initials: RE
authors.lastname: Elizondo-Omaña
mesh_terms: D000328:Adult; D000368:Aged; D064592:Autografts / Q000637:transplantation*; D002102:Cadaver; D003720:Densitometry; D004388:Dura Mater / Q000601:surgery* / Q000637:transplantation; D005205:Fascia / Q000637:transplantation*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010521:Periosteum / Q000637:transplantation*; D019651:Plastic Surgery Procedures / Q000379:methods*; D012886:Skull / Q000033:anatomy & histology; D013524:Surgical Flaps / Q000637:transplantation*; D013703:Temporal Muscle / Q000033:anatomy & histology; D055815:Young Adult
keywords: Dura mater; Duraplasty; Elastic fibers; Morphology; Pericranium; Temporalis fascia