title: miR-26a inhibits the proliferation of ovarian cancer cells via regulating CDC6 expression.
abstract: MicroRNAs (miRNA) play important regulatory roles in the occurrence and development of cancers. This study aimed to investigate the effects of miR-26a on the proliferation and apoptosis of ovarian cancer cells and explore the potential mechanism. qRT-PCR was performed to measure the miR-26a expression in 46 ovarian cancer tissues, and results showed miR-26a expression reduced significantly when compared with normal ovarian tissues (P<0.05). Moreover, miR-26a expression was related to the extent of cell differentiation and clinical stage of ovarian cancer (P<0.05). miR-26a mimic was transfected into SKOV3 cells and ES2 cells, and CCK8 assay, colony formation assay and flow cytometry showed miR-26a over-expression could significantly inhibit the proliferation of ovarian cancer cells and induce their apoptosis. Bioinformatics analysis revealed Cdc6 was a target gene of miR-26a. dual-luciferase assay and validation assay showed miR-26a could act on the 3'UTR of Cdc6 to regulate Cdc6 expression. These findings suggest that miR-26a may act on the 3'UTR of Cdc6 to regulate Cdc6 expression, which then inhibit the proliferation of ovarian cancer cells and induce their apoptosis. Our findings provide a new target for the diagnosis and therapy of ovarian cancer.
authors.affiliation: Department of Pathology, Henan Provincial People's Hospital Zhengzhou, China.
authors.forename: Ting-Yi
authors.initials: TY
authors.lastname: Sun
authors.affiliation: Department of Pathology, Henan Provincial People's Hospital Zhengzhou, China.
authors.forename: Hong-Jian
authors.initials: HJ
authors.lastname: Xie
authors.affiliation: Department of Pathology, Henan Provincial People's Hospital Zhengzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Pathology, Henan Provincial People's Hospital Zhengzhou, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pathology, Henan Provincial People's Hospital Zhengzhou, China.
authors.forename: Ling-Fei
authors.initials: LF
authors.lastname: Kong
keywords: Ovarian cancer; cell division cycle 6; miR-26a; proliferation