title: Ameliorating effect of Allium Sativum on high-fat diet induced fatty liver in albino rats.
abstract: OBJECTIVE
  To assess the hepatoprotective effect provided by fresh garlic on fatty liver induced by high-fat diet.
  
  
  METHODS
  This experimental study was carried out at BMSI, JPMC from October to November 2008. Thirty adult albino rats, 200-240 gram weight, were divided into three groups. Group A received control diet, Group B received high-fat diet (20 mg butter/100 gm diet) and Group C received high-fat diet with fresh garlic (20 mg butter with 6 gm fresh garlic/100 gm diet). The groups were further divided on the basis of duration of treatment, four weeks and eight weeks respectively. The rats were sacrificed, liver removed, weighed and relative liver weight calculated. Hepatic tissue was processed and tissue slides stained with haematoxylin and eosin.
  
  
  RESULTS
  There was significant increase in relative liver weight in group B animals as compared to the control animals, which decreased significantly in group C. Haematoxylin and eosin stained sections revealed ballooned hepatocytes having vesicular appearance with pyknotic nuclei in high-fat group which were preserved to a great extent in group C animals.
  
  
  CONCLUSIONS
  This study has shown that use of fresh garlic along with high-fat diet prevents its damaging effects on liver to a great extent.
authors.affiliation: Dr. Ayesha Qamar, M Phil, Department of Anatomy, Bahria University Medical and Dental College, Karachi - Pakistan.
authors.forename: Aisha
authors.initials: A
authors.lastname: Qamar
authors.affiliation: Dr. Ambreen Usmani, M Phil, Department of Anatomy, Bahria University Medical and Dental College, Karachi - Pakistan.
authors.forename: Ambreen
authors.initials: A
authors.lastname: Usmani
authors.affiliation: Dr. Humera Waqar, MBBS, Department of Anatomy, Bahria University Medical and Dental College, Karachi - Pakistan.
authors.forename: Humera
authors.initials: H
authors.lastname: Waqar
authors.affiliation: Dr. Asma Siddiqui, M Phil, Department of Anatomy, Bahria University Medical and Dental College, Karachi - Pakistan.
authors.forename: Asma
authors.initials: A
authors.lastname: Siddiqui
authors.affiliation: Dr. Hemant Kumar, M Phil, Department of Anatomy, Hamdard University, Karachi - Pakistan.
authors.forename: Hemant
authors.initials: H
authors.lastname: Kumar
keywords: Fatty liver; Garlic; High-fat diet