title: Simultaneous photoelectrochemical and visualized immunoassay of β-human chorionic gonadotrophin.
abstract: Herein, on the basis of the alkaline phosphate (ALP) induced reaction, a simultaneous photoelectrochemical (PEC) and visualized immunoassay has been established for the detection of β-human chorionic gonadotrophin (β-HCG). Specifically, in the proposed system, ALP stimulated the oxidative hydrolyzing transformation of 5-bromo-4-chloro-3-indoyl phosphate (BCIP) to an indigo precipitation, generating an insulating layer that impeded the interfacial mass and electron transfer and thus the photocurrent production. Meanwhile, a visualized detection could be performed according to the change of color intensity. Upon proper experimental conditions, the protocol possessed a detection range of 0.5-1000IU/L with a detection limit of (0.20±0.011)IU/L toward β-HCG. With high sensitivity and specificity, this work presents the first general protocol for simultaneous PEC and visualized detection, which could be easily extended to addressing numerous other targets.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Zheng-Yuan
authors.initials: ZY
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, Nanjing 210023, China. Electronic address: zww@nju.edu.cn.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, Nanjing University, Nanjing 210023, China. Electronic address: hychen@nju.edu.cn.
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry; D006063:Chorionic Gonadotropin / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry / Q000648:ultrastructure; D018036:Selenium Compounds / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D019187:Cadmium Compounds; D006063:Chorionic Gonadotropin; D018036:Selenium Compounds; C058667:cadmium selenide; D000469:Alkaline Phosphatase
keywords: Alkaline phosphate; Immunoassay; Photoelectrochemical; Visualized detection; β-Human chorionic gonadotrophin