title: Potent in vivo anticancer activity and stability of liposomes encapsulated with semi-purified Job's tear (Coix lacryma-jobi Linn.) extracts on human colon adenocarcinoma (HT-29) xenografted mice.
abstract: The in vivo anticancer activity and stability of liposomes encapsulated with semi-purified Job's tear (Coix lacryma-jobi Linn.) extracts (S5L), prepared by supercritical carbon dioxide fluid technique, on human colon adenocarcinoma (HT29) xenografted mice were investigated. For the stability and the physicochemical characteristics, S5L showed a high stability of pH, good dispersibility, small particle size and stable zeta potential. Liposomes can protect linoleic acid in the extract comparing with the free S5. S5L kept at 4 °C for 3 months showed the highest linoleic acid content of 63.50%, whereas at 45 °C, the lowest linoleic acid content of 42.66% was observed. The anticancer activity and toxicity on xenografted mice were observed for 14 days. At the end of the experiment, the relative tumor volume (RTV) in the S5L-treated xenografted mice showed a significant RTV reduction. The high dose of S5 and S5L were potent with the highest inhibition of tumor growth of 48.67 and 54.75%, which was 86.94% and 97.81% of 5-fluorouracil, respectively. The apoptotic activity was shown in xenografted mice treated with S5 at medium and high dose, S5L, 5-fluorouracil and commercial product. All treated xenografted mice showed no toxic signs and symptoms, abnormality of internal organs histopathology and blood chemistry. This study has demonstrated the high physicochemical stability of liposomes encapsulated with semi-purified Job's tear extract and their potent anticancer activity on human colon adenocarcinoma xenografted model with the potential for further development to anticolon cancer drug.
authors.affiliation: a Faculty of Pharmacy , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Mathukorn
authors.initials: M
authors.lastname: Sainakham
authors.affiliation: a Faculty of Pharmacy , Chiang Mai University , Chiang Mai , Thailand.|b Manose Health and Beauty Research Center , Meung Chiang Mai , Thailand.|c Faculty of Science and Technology , North Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Aranya
authors.initials: A
authors.lastname: Manosroi
authors.affiliation: d Department of Pure and Applied Chemistry , Faculty of Science and Technology, Tokyo University of Science , Chiba , Japan , and.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Abe
authors.affiliation: e Faculty of Medicine , Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Worapaka
authors.initials: W
authors.lastname: Manosroi
authors.affiliation: a Faculty of Pharmacy , Chiang Mai University , Chiang Mai , Thailand.|b Manose Health and Beauty Research Center , Meung Chiang Mai , Thailand.|c Faculty of Science and Technology , North Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Jiradej
authors.initials: J
authors.lastname: Manosroi
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D031742:Coix / Q000737:chemistry*; D003106:Colon / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy*; D004355:Drug Stability; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D010936:Plant Extracts; D005472:Fluorouracil
keywords: Colon cancer; Job’s tears; liposome; potent anticancer activity; xenografted mice