title: HspB8 mediates neuroprotection against OGD/R in N2A cells through the phosphoinositide 3-kinase/Akt pathway.
abstract: In a previous study, we found that Heat shock protein B8 (HspB8) overexpression could prevent the apoptosis and reduced cell viability induced by OGD/R and showed that the neuroprotective effect of HspB8 was mediated by inhibition of the mitochondrial apoptotic pathway. In recent study, HspB8 has been shown to protect the heart against ischemia/reperfusion (I/R) injury via activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. However, whether this protective effect applied to brain I/R injury remained unexplored. To further test the mechanism of HspB8's effects in brain, we used oxygen-glucose deprivation followed by reperfusion (OGD/R), an in vitro model of ischemia to examine the involvement of PI3K/Akt signaling by treating mouse neuroblastoma cells (N2A cells) (untransfected or transfected with an HspB8 expression vector) with the PI3K inhibitor LY294002 before OGD/R. Our results revealed that the apoptosis-suppressing effect of HspB8 was mediated by the PI3K/Akt pathway. Therefore, HspB8 protected the N2A cells against OGD/R insult, possibly by activating the PI3K/Akt signaling pathway.
authors.affiliation: Department of Neurology, 2nd Xiangya Hospital, Central South University, China. Electronic address: huzhiping2014@163.com.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Neurology, 2nd Xiangya Hospital, Central South University, China. Electronic address: binbin617@163.com.
authors.forename: Binbin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Emergency, 1st Xiangya Hospital, Central South University, China. Electronic address: moxiaoyes@136.com.
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Mo
authors.affiliation: Department of Neurology, 2nd Xiangya Hospital, Central South University, China. Electronic address: 85707398@qq.com.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000378:metabolism*; D045744:Cell Line, Tumor; D005947:Glucose / Q000378:metabolism; D050886:HSP20 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D051379:Mice; D018832:Molecular Chaperones; D009124:Muscle Proteins / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D010766:Phosphorylation; D061251:Primary Cell Culture; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D050886:HSP20 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C490206:Hspb8 protein, mouse; D018832:Molecular Chaperones; D009124:Muscle Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose; D010100:Oxygen
keywords: HspB8; Neuroprotection OGD/R; PI3K/Akt Pathway