title: Biosynthesis of AgNPs using Carica Papaya peel extract and evaluation of its antioxidant and antimicrobial activities.
abstract: Waste fruit peel mediated synthesis of silver nanoparticles (AgNPs) is a green chemistry approach that links nanotechnology and biotechnology. Using biological medium such as peel extract for the biosynthesis of nanoparticles is an ecofriendly and emerging scientific trend. With this back drop the present study focused on the biosynthesis of AgNPs using Carica Papaya peel extract (CPPE) and evaluation of its antimicrobial potentials of the nanoparticles against different human pathogens and to investigate the free radical scavenging activity. Water soluble antioxidant constituents present in Carica Papaya peel extract were mainly responsible for the reduction of silver ions to nanosized Ag particles. UV-vis spectral analysis shows surface plasmon resonance band at 430nm. The presence of active proteins and phenolic groups present in the biomass before and after reduction was identified by Fourier transform infrared spectroscopy. X-ray diffraction study shows the average size of the silver nanoparticles is in the range of 28nm, as well as revealed their face centered cubic structure. Atomic force microscope image gives the 3D topological characteristic of silver nanoparticles and the particle size ranges from 10 to 30nm. The average particle size distribution of silver nanoparticles is 161nm (Dynamic light scattering) and the corresponding average zeta potential value is -20.5mV, suggesting higher stability of silver nanoparticles. Biologically synthesized nanoparticles efficiently inhibited pathogenic organisms both gram-positive and gram-negative bacteria. The biosynthesized nanoparticles might serve as a potent antioxidant as revealed by DPPH and ABTS+assay.
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India.
authors.forename: T
authors.initials: T
authors.lastname: Kokila
authors.affiliation: Physics Wing DDE, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India. Electronic address: psrddephyau@gmail.com.
authors.forename: P S
authors.initials: PS
authors.lastname: Ramesh
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India.
authors.forename: D
authors.initials: D
authors.lastname: Geetha
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D029441:Carica; D005638:Fruit; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D010936:Plant Extracts; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D010936:Plant Extracts; D012834:Silver
keywords: Antioxidant and antimicrobial activity; Biosynthesis; Carica Papaya peel extract; Silver nanoparticles