title: Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides.
abstract: An actinomycete strain, designated YIM T102(T), was isolated from the rhizospheric soil of Psammosilene tunicoides W. C. Wu et C. Y. Wu collected from Lijiang, Yunnan Province, China. The taxonomic position of the new isolate was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM T102(T) belongs to the genus Streptomyces. Strain YIM T102(T) was most closely related to Streptomyces eurocidicus NRRL B-1676(T) with a pairwise 16S rRNA gene sequence similarity of 98.9 %. However, DNA-DNA relatedness value between strain YIM T102(T) and S. eurocidicus NBRC 13491(T) was found to be 37.8 ± 1.8 %. The menaquinone composition detected for strain YIM T102(T) was MK-9 (H6) and MK-9 (H8), while the major fatty acids were summed feature 4 (38.0 %), anteiso-C15:0 (13.1 %), iso-C16:0 (10.1 %), summed feature 3 (9.8 %) and C16:0 (9.0 %) and iso-C15:0 (5.2 %). The whole-cell hydrolysates contained galactose, glucose, ribose and mannose, along with LL-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 70.7 mol%. Strain YIM T102(T) also exhibited antagonistic activity against Alternaria alternata, Alternaria brassicae and Colletotrichum nicotianae Averna, based on the findings from the comparative analyses of phenotypic and genotypic characteristics; it is proposed that strain YIM T102 represents a novel species of the genus Streptomyces, for which the name Streptomyces zhihengii sp. nov. is proposed. The type strain is YIM T102(T) (=KCTC 39115(T) = DSM 42176(T) = CGMCC 4.7248(T)).
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China.|College of Landscape Architecture, Southwest Forestry University, Kunming, 650224, China.
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Huang
authors.affiliation: College of Landscape Architecture, Southwest Forestry University, Kunming, 650224, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Fei
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
authors.forename: Nimaichand
authors.initials: N
authors.lastname: Salam
authors.affiliation: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.affiliation: College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.|Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62511, Egypt.
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: College of Landscape Architecture, Southwest Forestry University, Kunming, 650224, China. haiquanl@163.com.
authors.forename: Hai-Quan
authors.initials: HQ
authors.lastname: Huang
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China. liwenjun3@mail.sysu.edu.cn.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D029748:Caryophyllaceae / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid
keywords: Lijiang; Psammosilene tunicoides; Rhizosphere soil; Streptomyces zhihengii sp. nov.