title: EFFECT OF STORAGE TIME AND STORAGE CONDITIONS ON ANTIBODY DETECTION IN BLOOD SAMPLES COLLECTED ON FILTER PAPER.
abstract: Using filter paper to collect blood from wildlife for antibody analysis can be a powerful technique to simplify the collection, transport, and storage of blood samples. Despite these advantages, there are limited data that detail how long these samples can be stored and how storage conditions affect antibody longevity. We used blood samples collected on filter paper from coyotes experimentally infected with Yersinia pestis to determine optimum sample storage conditions over time. Blood samples collected on filter paper were stored for 454 d or more in four groups: 1) at ambient temperature and at ambient relative humidity, 2) at ambient temperature with desiccant, 3) at 4 C with desiccant, and 4) at -20 C with desiccant. Samples stored at 4 C or -20 C with desiccant had detectable antibody for a longer period of time than the samples stored at room temperature.
authors.affiliation: 1   US Department of Agriculture, Wildlife Services, National Wildlife Research Center, 4101 Laporte Ave., Fort Collins, Colorado 80521, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Bevins
authors.affiliation: 2   Centers for Disease Control and Prevention, 3150 Rampart Rd., Fort Collins, Colorado 80521, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Pappert
authors.affiliation: 2   Centers for Disease Control and Prevention, 3150 Rampart Rd., Fort Collins, Colorado 80521, USA.
authors.forename: John
authors.initials: J
authors.lastname: Young
authors.affiliation: 1   US Department of Agriculture, Wildlife Services, National Wildlife Research Center, 4101 Laporte Ave., Fort Collins, Colorado 80521, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Schmit
authors.affiliation: 1   US Department of Agriculture, Wildlife Services, National Wildlife Research Center, 4101 Laporte Ave., Fort Collins, Colorado 80521, USA.
authors.forename: Dennis
authors.initials: D
authors.lastname: Kohler
authors.affiliation: 3   Colorado State University, 1683 Campus Delivery, Fort Collins, Colorado 80523-1683, USA.
authors.forename: Laurie
authors.initials: L
authors.lastname: Baeten
mesh_terms: D000818:Animals; D000835:Animals, Wild; D000906:Antibodies / Q000032:analysis*; D046029:Coyotes; D010209:Paper; D013048:Specimen Handling; D013696:Temperature; D015010:Yersinia pestis
chemical_list: D000906:Antibodies
keywords: Antibody; Nobuto; Yersinia pestis; blood; coyote; filter paper; plague