title: Assessment of the effects of oseltamivir and indomethacin on dopamine, 5-HIAA, and some oxidative stress markers in stomach and brain of Salmonella typhimurium-infected rats.
abstract: The purpose of this study was to measure the effect of oseltamivir and indomethacin on dopamine and 5-HIAA levels and some oxidative biomarkers in brain and stomach of young rats in conditions of infection.
  
  
  
  Female Sprague Dawley rats in absence or presence of a live culture of Salmonella typhimurium (S.Typh), were treated as follows: PBS, group 1 (control); oseltamivir (100 mg/kg), group 2; indomethacin (67 µg/kg) group 3; oseltamivir (100 mg/kg) + indomethacin (67 µg/kg), group 4. The drugs were administered intraperitoneally every 24 hr for 5 days while S. Typh was give orally in the first and third day. C-reactive proteins was measured in blood on sacrifice, and from brain extract, dopamine and 5-HIAA levels as well as GSH, calcium, and H2O2 and total ATPase activity were measured by validated methods.
  
  
  
  Dopamine increased significantly in cortex and cerebellum/medulla oblongata of groups that received indomethacin and oseltamivir. 5-HIAA increased significantly in all groups that received S.Typh. H2O2 decreased significantly in cortex regions of animals that received oseltamivir and indomethacin in presence of S.Typh. Total ATPase increased significantly in cortex and hemispheres of groups that received oseltamivir as well as in cerebellum/medulla oblongata and stomach of animals that received oseltamivir and indomethacin combined with S.Typh. GSH increased and calcium decreased significantly in stomach of animals that received oseltamivir or indomethacin alone or combined with S.Typh.
  
  
  
  These results demonstrate the association between inflammatory response, oxidative stress, dopaminergic, and serotonergic metabolism in an experimental inflammatory animal model.
authors.affiliation: Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), Mexico.
authors.forename: David Calderón
authors.initials: DC
authors.lastname: Guzmán
authors.affiliation: Laboratorio de Bacteriología Experimental INP, Mexico.
authors.forename: Maribel Ortiz
authors.initials: MO
authors.lastname: Herrera
authors.affiliation: Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), Mexico.
authors.forename: Norma Osnaya
authors.initials: NO
authors.lastname: Brizuela
authors.affiliation: Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), Mexico.
authors.forename: Gerardo Barragán
authors.initials: GB
authors.lastname: Mejía
authors.affiliation: Laboratorio de Farmacología INP, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Francisca Trujillo
authors.initials: FT
authors.lastname: Jiménez
authors.affiliation: Laboratorio de Farmacología INP, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Ernestina Hernández
authors.initials: EH
authors.lastname: García
authors.affiliation: Laboratorio de Farmacología INP, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Hugo Juárez
authors.initials: HJ
authors.lastname: Olguín
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D006861:Hydrogen Peroxide; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology*; D053139:Oseltamivir / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012486:Salmonella typhimurium; D013270:Stomach / Q000382:microbiology; D014435:Typhoid Fever
chemical_list: D053139:Oseltamivir; D006897:Hydroxyindoleacetic Acid; D006861:Hydrogen Peroxide; D004298:Dopamine; D007213:Indomethacin