title: Molecular Characterization and Sex Distribution of Chemosensory Receptor Gene Family Based on Transcriptome Analysis of Scaeva pyrastri.
abstract: Chemosensory receptors play key roles in insect behavior. Thus, genes encoding these receptors have great potential for use in integrated pest management. The hover fly Scaeva pyrastri (L.) is an important pollinating insect and a natural enemy of aphids, mainly distributed in the Palearctic and Nearctic regions. However, a systematic identification of their chemosensory receptor genes in the antennae has not been reported. In the present study, we assembled the antennal transcriptome of S. pyrastri by using Illumina sequencing technology. Analysis of the transcriptome data identified 60 candidate chemosensory genes, including 38 for odorant receptors (ORs), 16 for ionotropic receptors (IRs), and 6 for gustatory receptors (GRs). The numbers are similar to those of other Diptera species, suggesting that we were able to successfully identify S. pyrastri chemosensory genes. We analyzed the expression patterns of all genes by using reverse transcriptase PCR (RT-PCR), and found that some genes exhibited sex-biased or sex-specific expression. These candidate chemosensory genes and their tissue expression profiles provide information for further studies aimed at fully understanding the molecular basis behind chemoreception-related behaviors in S. pyrastri.
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Li
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei, China.
authors.forename: Xiu-Yun
authors.initials: XY
authors.lastname: Zhu
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei, China.
authors.forename: Dao-Gui
authors.initials: DG
authors.lastname: Deng
authors.affiliation: College of Life Sciences, Huaibei Normal University, Huaibei, China.
authors.forename: Ya-Nan
authors.identifier: 0000-0001-7038-2292
authors.initials: YN
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D004175:Diptera / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D017678:Sex Distribution; D059467:Transcriptome / Q000235:genetics
chemical_list: D019476:Insect Proteins