title: BK polyomavirus association with colorectal cancer development.
abstract: The development of human neoplasms can be provoked by exposure to one of several viruses. Burkitt lymphoma, cervical carcinoma, and hepatocellular carcinoma are associated with Epstein-Barr, human papilloma, and hepatitis B virus infections, respectively. Over the past three decades, many studies have attempted to establish an association between colorectal cancer and viruses, with debatable results. The aim of the present research was to assess the presence of BK polyomavirus (BKV) DNA and protein in colorectal cancer samples from patients in the Western Province of Saudi Arabia. DNA extracted from archival samples of colorectal cancer tissues was analyzed for BKV sequences using polymerase chain reaction (PCR)-based techniques. In addition, expression of a BKV protein was assessed using immunohistochemical staining. None of the tumor and control samples examined tested positive for BKV DNA in PCR assays. Furthermore, immunohistochemical staining failed to detect viral proteins in both cancer and control specimens. These results may indicate that BKV is not associated with the development of colorectal adenocarcinoma in patients in the Western Province of Saudi Arabia.
authors.affiliation: Department of Pathology, Faculty of Medicine, Rabigh Branch, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: M N
authors.initials: MN
authors.lastname: Khabaz
authors.affiliation: King Fahd Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: T
authors.initials: T
authors.lastname: Nedjadi
authors.affiliation: Centre of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: M A
authors.initials: MA
authors.lastname: Gari
authors.affiliation: Department of Pathology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: J A
authors.initials: JA
authors.lastname: Al-Maghrabi
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Rabigh Branch, King Abdulaziz University, Jeddah, Saudi Arabia.|Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: H M
authors.initials: HM
authors.lastname: Atta
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Rabigh Branch, King Abdulaziz University, Jeddah, Saudi Arabia.|Liver Institute, Menoufia University, Al-Minufiya, Egypt.
authors.forename: A A
authors.initials: AA
authors.lastname: Basuni
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.|Department of Public Health and Community Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: D A
authors.initials: DA
authors.lastname: Elderwi
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012529:Saudi Arabia; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins