title: Bioinformatics analysis and construction of phylogenetic tree of aquaporins from Echinococcus granulosus.
abstract: Cyst echinococcosis caused by the matacestodal larvae of Echinococcus granulosus (Eg), is a chronic, worldwide, and severe zoonotic parasitosis. The treatment of cyst echinococcosis is still difficult since surgery cannot fit the needs of all patients, and drugs can lead to serious adverse events as well as resistance. The screen of target proteins interacted with new anti-hydatidosis drugs is urgently needed to meet the prevailing challenges. Here, we analyzed the sequences and structure properties, and constructed a phylogenetic tree by bioinformatics methods. The MIP family signature and Protein kinase C phosphorylation sites were predicted in all nine EgAQPs. α-helix and random coil were the main secondary structures of EgAQPs. The numbers of transmembrane regions were three to six, which indicated that EgAQPs contained multiple hydrophobic regions. A neighbor-joining tree indicated that EgAQPs were divided into two branches, seven EgAQPs formed a clade with AQP1 from human, a "strict" aquaporins, other two EgAQPs formed a clade with AQP9 from human, an aquaglyceroporins. Unfortunately, homology modeling of EgAQPs was aborted. These results provide a foundation for understanding and researches of the biological function of E. granulosus.
authors.affiliation: Department of Pathogenic Biology, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pathogenic Biology, Chongqing Medical University, Chongqing, 400016, People's Republic of China. yebina@sohu.com.|Research Center for Molecule Medicine and Tumor, Chongqing Medical University, Chongqing, People's Republic of China. yebina@sohu.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Ye
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D055808:Drug Discovery / Q000379:methods*; D004443:Echinococcosis / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D010766:Phosphorylation; D010802:Phylogeny
chemical_list: D020346:Aquaporins; D008565:Membrane Proteins
keywords: Aquaporins; Bioinformatics; Echinococcus granulosus (Eg); Phylogenetic tree