title: Effect of tris(3-hydroxy-4-pyridinonate) iron(III) complexes on iron uptake and storage in soybean (Glycine max L.).
abstract: Iron deficiency chlorosis (IDC) is a serious environmental problem affecting the growth of several crops in the world. The application of synthetic Fe(III) chelates is still one of the most common measures to correct IDC and the search for more effective Fe chelates remains an important issue. Herein, we propose a tris(3-hydroxy-4-pyridinonate) iron(III) complex, Fe(mpp)3, as an IDC corrector. Different morphological, biochemical and molecular parameters were assessed as a first step towards understanding its mode of action, compared with that of the commercial fertilizer FeEDDHA. Plants treated with the pyridinone iron(III) complexes were significantly greener and had increased biomass. The total Fe content was measured using ICP-OES and plants treated with pyridinone complexes accumulated about 50% more Fe than those treated with the commercial chelate. In particular, plants supplied with compound Fe(mpp)3 were able to translocate iron from the roots to the shoots and did not elicit the expression of the Fe-stress related genes FRO2 and IRT1. These results suggest that 3,4-HPO iron(III) chelates could be a potential new class of plant fertilizing agents.
authors.affiliation: Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Carla S
authors.initials: CS
authors.lastname: Santos
authors.affiliation: Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal; GreenUP/CITAB-UP & DGAOT, Faculty of Sciences, University of Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 7, 4485-661 Vairão, Portugal.
authors.forename: Susana M P
authors.initials: SM
authors.lastname: Carvalho
authors.affiliation: REQUIMTE-UCIBIO, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, 4069-007 Porto, Portugal.
authors.forename: Andreia
authors.initials: A
authors.lastname: Leite
authors.affiliation: REQUIMTE-UCIBIO, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, 4069-007 Porto, Portugal.
authors.forename: Tânia
authors.initials: T
authors.lastname: Moniz
authors.affiliation: Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Mariana
authors.initials: M
authors.lastname: Roriz
authors.affiliation: Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: António O S S
authors.initials: AO
authors.lastname: Rangel
authors.affiliation: REQUIMTE-UCIBIO, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal. Electronic address: mcrangel@fc.up.pt.
authors.forename: Maria
authors.initials: M
authors.lastname: Rangel
authors.affiliation: Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal. Electronic address: mvasconcelos@porto.ucp.pt.
authors.forename: Marta W
authors.initials: MW
authors.lastname: Vasconcelos
mesh_terms: D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D038181:FMN Reductase / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D008024:Ligands; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008903:Minerals / Q000378:metabolism; D063245:Plant Development / Q000187:drug effects; D010935:Plant Diseases; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology*; D013025:Glycine max / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013057:Spectrum Analysis
chemical_list: D005290:Ferric Compounds; D007502:Iron Chelating Agents; D008024:Ligands; D008903:Minerals; D010940:Plant Proteins; D011725:Pyridines; C000616031:tris(2-methyl-3-hydroxy-4-pyridinone)iron(III); D002734:Chlorophyll; D008315:Malondialdehyde; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase
keywords: 3-Hydroxy-4-pyridinones; Chlorosis; FRO2; Fe-chelates; Ferritin; Glycine max; IRT1