title: [Effects of fine particulate matter from on bronchial epithelial cells].
abstract: BACKGROUND
  Ambient air contains a variety of pollutants including solid particles. Human bronchial epithelial cells (HBEC) play a key role following inhalation of these toxic particles. In a cell culture system of HBEC, we investigated the effects of several fractions of Lyons air-derived particles on cell viability/proliferation and production of the inflammatory mediators IL-8 and TGF-β.
  
  
  METHODS
  Air particles were collected in Lyon (France) by filtration of the air and impaction on filters located on a tapered element oscillating microbalance. Several diameter-based fractions of particles were prepared. HBEC were then exposed to different concentrations of these fractions.
  
  
  RESULTS
  HBEC survival was not altered after 48hours of culture in the presence of particles regardless of their diameter and concentration. A dose-dependent inhibitory effect on cell growth was observed for all fractions. The particles caused an increase of both TGF-ß and Il-8 protein levels as a function of their diameters and/or concentrations.
  
  
  CONCLUSIONS
  Air-derived particle fractions exhibit both an inhibitory effect on cell proliferation and a pro-inflammatory activity on HBEC.
authors.affiliation: Service de pneumologie, centre hospitalier Lyon Sud, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Aloui
authors.affiliation: Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France; Service de pneumologie, hôpital de la Croix-Rousse, 69000 Lyon, France.
authors.forename: F
authors.initials: F
authors.lastname: Magne
authors.affiliation: Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France; Service de pneumologie, hôpital de la Croix-Rousse, 69000 Lyon, France.
authors.forename: G
authors.initials: G
authors.lastname: Devouassoux
authors.affiliation: Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France.
authors.forename: J
authors.initials: J
authors.lastname: Deverchere
authors.affiliation: Direction de l'écologie urbaine, mairie de Lyon, 69205 Lyon cedex 01, France.
authors.forename: P
authors.initials: P
authors.lastname: Ritter
authors.affiliation: Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France.
authors.forename: A
authors.initials: A
authors.lastname: Bentaher
authors.affiliation: Inserm U1111, CNRS UMR5308, inflammation et immunité de l'épithélium respiratoire, faculté de médecine et de Maïeutique Lyon Sud - Charles-Mérieux, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite, France; Service de pneumologie, centre hospitalier Lyon Sud, 165, chemin du Grand-Revoyet, 69495 Pierre-Bénite cedex, France. Electronic address: yves.pacheco@univ-lyon1.fr.
authors.forename: Y
authors.initials: Y
authors.lastname: Pacheco
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D005602:France; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D010316:Particle Size; D052638:Particulate Matter / Q000009:adverse effects / Q000494:pharmacology*; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000393:Air Pollutants; C508606:CXCL8 protein, human; D016209:Interleukin-8; D052638:Particulate Matter; D016212:Transforming Growth Factor beta
keywords: Bronchial epithelial cells; Cellules épithéliales bronchiques; Cytokines; Fine particles; IL-8; Particules fines; TGF-β