title: A comparative study of mucilage and pulp polysaccharides from tamarillo fruit (Solanum betaceum Cav.).
abstract: A comparative study of mucilage (locular tissue) and pulp polysaccharides from ripe tamarillo fruits (Solanum betaceum Cav.) was carried out. After aqueous and alkaline extractions and various purification steps (freeze-thaw and α-amylase - EC 3.2.1.1 treatments, Fehling precipitation and ultrafiltration through 50 kDa cut-off membrane), the obtained fractions from mucilage were analyzed by sugar composition, HPSEC, and NMR spectroscopy analyses. The results showed that the mucilage of tamarillo contains a highly methoxylated homogalacturonans mixed with type I arabinogalactans, a linear (1 → 5)-linked α-L-arabinan, and a linear (1 → 4)-β-D-xylan. A comparison with polysaccharides extracted from the pulp revealed that differences were observed in the yield and in the ratio of extracted polysaccharides. Moreover, structural differences between pulp and mucilage polysaccharides were also observed, such as in the length of side chains of the pectins, and in the degree of branching of the xylans.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, PR, Brazil.
authors.forename: Georgia Erdmann
authors.initials: GE
authors.lastname: do Nascimento
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, PR, Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, PR, Brazil. Electronic address: lucimaramcc@ufpr.br.
authors.forename: Lucimara M C
authors.initials: LM
authors.lastname: Cordeiro
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D005638:Fruit / Q000378:metabolism*; D009005:Monosaccharides / Q000032:analysis; D010368:Pectins / Q000302:isolation & purification; D062087:Plant Mucilage / Q000378:metabolism*; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D032322:Solanum / Q000378:metabolism*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D009005:Monosaccharides; D062087:Plant Mucilage; D011134:Polysaccharides; D014867:Water; C007916:hemicellulose; D010368:Pectins
keywords: Arabinan; Arabinogalactan; Mucilage; Pectins; Solanum betaceum; Tamarillo; Xylan