title: Expansion of NK Cells Using Genetically Engineered K562 Feeder Cells.
abstract: Natural killer (NK) cells can be expanded upon activation by proliferative cytokines (such as IL-2 and IL-15). The NK cell expansion can be greatly enhanced by proteins from feeder cells such as tumor cell lines or PBMCs. Therefore, coculture systems of irradiated feeder cells and NK cells in media containing IL-2 and IL-15 have been developed to generate large numbers of NK cells, although NK cell expansion protocol using anti-CD3 antibody (OKT-3) without feeder cells has also been developed. Commonly used feeder cell lines are RPMI8866, Epstein-Barr lymphoblastoid cell line (EBV-LCL), and K562. Stimulation with NK-sensitive K562 cells is known to augment NK cell proliferation to IL-2, IL-15, and IL-21 in combination.Recently, remarkable NK cell-expansion rates are achieved when genetically engineered (GE) feeder cells are used. Dr. Dario Campana's group found that membrane-bound IL-15 and 4-1BBL, coexpressed by K562 cells, acted synergistically to augment K562-specific NK stimulatory capacity, resulting in vigorous expansion of peripheral blood CD56(+) CD3(-) NK cells without concomitant growth of T lymphocytes. Here, we describe an in vitro expansion method of human NK cells among PBMCs by coculturing with GE_K562 cells.
authors.affiliation: Center for Creative Biomedical Scientists, Chonnam National University, Gwangju, South Korea.
authors.forename: Minh-Trang Thi
authors.initials: MT
authors.lastname: Phan
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Companion & Laboratory Animal Science, College of Industrial Science, Kongju National University, Yesan, Chungnam, South Korea.
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul, 135-710, South Korea. duck.cho@samsung.com.
authors.forename: Duck
authors.initials: D
authors.lastname: Cho
mesh_terms: D053330:4-1BB Ligand / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D061252:Feeder Cells / Q000166:cytology* / Q000276:immunology; D005818:Genetic Engineering; D006801:Humans; D019409:Interleukin-15 / Q000378:metabolism*; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology*; D008213:Lymphocyte Activation
chemical_list: D053330:4-1BB Ligand; D019409:Interleukin-15
keywords: Expansion; Feeder cells; K562; Natural killer cell