title: Complete sequence of human mitochondrial DNA obtained by combining multiple displacement amplification and next-generation sequencing on a single oocyte.
abstract: Mitochondrial DNA (mtDNA) plays a key role in the development of a competent oocyte. In this study, the complete mtDNA sequence obtained for the first time by multiple displacement amplification approach in combination with next-generation sequencing from a single human oocyte is reported (GenBank accession no. KT364276). The analysis of oocyte mitochondrial mutations could provide a better understanding of the genetic variants correlated with the oocyte quality.
authors.affiliation: a Research and Development Biotechnology Unit , Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" , Teramo , Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Ancora
authors.affiliation: a Research and Development Biotechnology Unit , Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" , Teramo , Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Orsini
authors.affiliation: b Facoltà di Medicina Veterinaria , Università di Teramo , Teramo , Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Colosimo
authors.affiliation: a Research and Development Biotechnology Unit , Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" , Teramo , Italy.
authors.forename: Maurilia
authors.initials: M
authors.lastname: Marcacci
authors.affiliation: b Facoltà di Medicina Veterinaria , Università di Teramo , Teramo , Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Russo
authors.affiliation: c Casa di Cura Privata Synergo Pierangeli, Spatocco , Chieti , Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: De Santo
authors.affiliation: d Scuola di Medicina e Scienze della Salute , Università "G. D'Annunzio" Chieti-Pescara , Chieti , Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: D'Aurora
authors.affiliation: d Scuola di Medicina e Scienze della Salute , Università "G. D'Annunzio" Chieti-Pescara , Chieti , Italy.
authors.forename: Liborio
authors.initials: L
authors.lastname: Stuppia
authors.affiliation: b Facoltà di Medicina Veterinaria , Università di Teramo , Teramo , Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Barboni
authors.affiliation: a Research and Development Biotechnology Unit , Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" , Teramo , Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Cammà
authors.affiliation: d Scuola di Medicina e Scienze della Salute , Università "G. D'Annunzio" Chieti-Pescara , Chieti , Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Gatta
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D004272:DNA, Mitochondrial; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009154:Mutation; D009865:Oocytes
chemical_list: D004272:DNA, Mitochondrial
keywords: Heteroplasmic mutations; human oocyte; mitochondrial DNA; next-generation sequencing