title: Diagnostic accuracy of presepsin (sCD14-ST) and procalcitonin for prediction of bacteraemia and bacterial DNAaemia in patients with suspected sepsis.
abstract: Early diagnosis and prompt targeted therapy are essential for septic patients' outcome. Procalcitonin (PCT) has been shown to predict bacteraemia and bacterial DNAaemia. Presepsin, the circulating soluble form of CD14 subtype, increases in response to bacterial infections, and is considered a new, emerging, early marker for sepsis. We evaluated the diagnostic accuracy of presepsin in predicting bacteraemia and bacterial DNAaemia in 92 patients with suspected sepsis, and we compared it with that of PCT and C-reactive protein (CRP). Presepsin median values were significantly higher in bacteraemic vs non-bacteraemic patients [1290 pg ml-1, interquartile range (IQR) 1005-2041 vs 659 pg ml-1, IQR 381-979; P<0.001] and in patients with vs patients without bacterial DNAaemia (1297 pg ml-1, IQR 1001-2046 vs 665 pg ml-1, IQR 381-940; P<0.001). Receiver operating characteristics analysis showed an area under the curve (AUC) for presepsin of 0.788 [95 % confidence interval (CI): 0.687-0.889; P<0.001] in predicting bacteraemia and of 0.777 (95 % CI: 0.676-0.878; P<0.001) in predicting bacterial DNAaemia, lower, but not significantly different, than those of PCT (0.876, P=0.12 and 0.880, P=0.07, respectively). Both biomarkers performed significantly better than CRP, which had an AUC for bacteraemia of 0.602 and for DNAaemia of 0.632 (all P values <0.05). In conclusion, in patients with suspected sepsis, presepsin and PCT showed a good diagnostic accuracy in predicting both bacteraemia and bacterial DNAaemia, superior to CRP.
authors.affiliation: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors.forename: Christian
authors.initials: C
authors.lastname: Leli
authors.affiliation: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Ferranti
authors.affiliation: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Marrano
authors.affiliation: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors.forename: Zainab Salim
authors.initials: ZS
authors.lastname: Al Dhahab
authors.affiliation: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bozza
authors.affiliation: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors.forename: Elio
authors.initials: E
authors.lastname: Cenci
authors.affiliation: Microbiology Section, Department of Experimental Medicine, University of Perugia, Perugia, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Mencacci
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000032:analysis; D002116:Calcitonin / Q000097:blood*; D004269:DNA, Bacterial / Q000097:blood*; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012372:ROC Curve; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004269:DNA, Bacterial; D018950:Lipopolysaccharide Receptors; D010446:Peptide Fragments; C000594167:presepsin protein, human; D002116:Calcitonin; D002097:C-Reactive Protein