title: A Novel Nanosilver/Nanosilica Hydrogel for Bone Regeneration in Infected Bone Defects.
abstract: Treating bone defects in the presence of infection is a formidable clinical challenge. The use of a biomaterial with the dual function of bone regeneration and infection control is a novel therapeutic approach to this problem. In this study, we fabricated an innovative, dual-function biocomposite hydrogel containing nanosilver and nanosilica (nAg/nSiO2) particles and evaluated its characteristics using FT-IR, SEM, swelling ratio, and stiffness assays. The in vitro antibacterial analysis showed that this nAg/nSiO2 hydrogel inhibited both Gram-positive and Gram-negative bacteria. In addition, this nontoxic material could promote osteogenic differentiation of rat bone marrow stromal cells (BMSCs). We then created infected bone defects in rat calvaria in order to evaluate the function of the hydrogel in vivo. The hydrogel demonstrated effective antibacterial ability while promoting bone regeneration in these defects. Our results indicate that this nAg/nSiO2 hydrogel has the potential to both control infection and to promote bone healing in contaminated defects.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.|Department of Mechanical Engineering, Faculty of Engineering and Department of Biochemistry & Genetics, Faculty of Medicine and Manitoba Institute of Child Health, University of Manitoba , Winnipeg, Manitoba R3E 3P4, Canada.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
authors.forename: Yunshu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
authors.forename: Yongyue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Mechanical Engineering, Faculty of Engineering and Department of Biochemistry & Genetics, Faculty of Medicine and Manitoba Institute of Child Health, University of Manitoba , Winnipeg, Manitoba R3E 3P4, Canada.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Xing
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000592:standards; D001861:Bone Regeneration / Q000187:drug effects*; D002478:Cells, Cultured; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D012822:Silicon Dioxide / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D012834:Silver; D012822:Silicon Dioxide
keywords: bone defects; hydrogel; infection; nanosilica; nanosilver