title: Analysis of α1 and α2 globin genes among patients with hemoglobin Adana in Malaysia.
abstract: Hemoglobin (Hb) Adana [HBA2: c179G>A (or HBA1); p.Gly60Asp] is a non-deletional α-thalassemia variant found in Malaysia. An improvement in the molecular techniques in recent years has made identification of Hb Adana much easier. For this study, a total of 26 Hb Adana α-thalassemia intermedia and 10 Hb Adana trait blood samples were collected from patients. Common deletional and non-deletional α-thalassemia genotypes were determined using multiplex gap polymerase chain reaction (PCR) and multiplex ARMS PCR techniques. Identification of the Hb Adana location on the α-globin gene was carried out using genomic sequencing and the location of the mutation was confirmed via restriction fragment length polymorphism-PCR. Among the 36 samples, 24 (66.7%) had the -α(3.7)/α(Cd59)α mutation, while the -α(3.7)/α(Cd59)α mutation accounted for 2 samples (5.6%) and the remaining 10 (27.8%) samples were α/α(Cd59)α. All 36 samples were found to have the Hb Adana mutation on the α2-globin gene. The position of the α-globin gene mutation found in our cases was similar to that reported in Indonesia (16%) but not to that in Turkey (0.6%). Our results showed that the Hb Adana mutation was preferentially present in the α2-globin genes in Malays compared to the other ethnicities in Malaysia. Thus, the Malays might have similar ancestry based on the similarities in the Hb Adana position.
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: T Y
authors.initials: TY
authors.lastname: Lee
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: M I
authors.initials: MI
authors.lastname: Lai
authors.affiliation: Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: P
authors.initials: P
authors.lastname: Ismail
authors.affiliation: Malaysian Research Institute on Ageing, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: V
authors.initials: V
authors.lastname: Ramachandran
authors.affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: J A M A
authors.initials: JA
authors.lastname: Tan
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia.
authors.forename: L K
authors.initials: LK
authors.lastname: Teh
authors.affiliation: Department of Pathology, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia.
authors.forename: R
authors.initials: R
authors.lastname: Othman
authors.affiliation: Department of Pathology, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia.
authors.forename: N H
authors.initials: NH
authors.lastname: Hussein
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: E
authors.initials: E
authors.lastname: George
mesh_terms: D006441:Hemoglobin A / Q000235:genetics*; D006455:Hemoglobins, Abnormal / Q000235:genetics*; D006801:Humans; D008296:Malaysia; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D017085:alpha-Thalassemia / Q000208:ethnology / Q000235:genetics*
chemical_list: D006455:Hemoglobins, Abnormal; C083925:hemoglobin Adana; D006441:Hemoglobin A