title: Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal.
abstract: The lipopolysaccharide of Escherichia coli O156 was degraded under mild acidic and alkaline conditions and the resulting polysaccharides were studied by sugar analysis and (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: where Rpyr indicates R-configurated pyruvic acid acetal. Minor O-acetyl groups also were present and tentatively localized on the Gal residues. The gene cluster for biosynthesis of the O-antigen of E. coli O156 was analyzed and shown to be consistent with the O-polysaccharide structure.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Duan
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Guo
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: yknirel@gmail.com.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*; D011773:Pyruvates / Q000737:chemistry*
chemical_list: D019081:O Antigens; D011773:Pyruvates; C064986:pyruvic acid acetal
keywords: Bacterial polysaccharide structure; Escherichia coli; O-antigen; O-antigen gene cluster; O-polysaccharide