title: New flow cytometry approaches in equine andrology.
abstract: Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the number of tests that can be potentially used in the evaluation of sperm physiology has increased accordingly. In this review, recent advances in the evaluation of stallion spermatozoa will be discussed. These new techniques in flow cytometry are able to simultaneously measure damage to different sperm regions and/or changes in functionality.
authors.affiliation: University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Cáceres, Spain. Electronic address: fjuanpvega@unex.es.
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Peña
authors.affiliation: Reproduction and Obstetrics, Department of Animal Medicine and Surgery, University of León, León, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ortega Ferrusola
authors.affiliation: University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Cáceres, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Martín Muñoz
mesh_terms: D000818:Animals; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D006736:Horses / Q000502:physiology*; D008297:Male; D055101:Semen Analysis / Q000662:veterinary*
keywords: Multiparametric flow cytometry; Spermatozoon; Stallion