title: Magnetic particle spectroscopy allows precise quantification of nanoparticles after passage through human brain microvascular endothelial cells.
abstract: Crossing the blood-brain barrier is an urgent requirement for the treatment of brain disorders. Superparamagnetic iron oxide nanoparticles (SPIONs) are a promising tool as carriers for therapeutics because of their physical properties, biocompatibility, and their biodegradability. In order to investigate the interaction of nanoparticles with endothelial cell layers in detail, in vitro systems are of great importance. Human brain microvascular endothelial cells are a well-suited blood-brain barrier model. Apart from generating optimal conditions for the barrier-forming cell units, the accurate detection and quantification of SPIONs is a major challenge. For that purpose we use magnetic particle spectroscopy to sensitively and directly quantify the SPION-specific iron content. We could show that SPION concentration depends on incubation time, nanoparticle concentration and location. This model system allows for further investigations on particle uptake and transport at cellular barriers with regard to parameters including particles' shape, material, size, and coating.
authors.affiliation: Abteilung Hämatologie und Internistische Onkologie, Universitätsklinikum Jena, Erlanger Allee 101, D-07747 Jena, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Gräfe
authors.forename: I
authors.initials: I
authors.lastname: Slabu
authors.forename: F
authors.initials: F
authors.lastname: Wiekhorst
authors.forename: C
authors.initials: C
authors.lastname: Bergemann
authors.forename: F
authors.initials: F
authors.lastname: von Eggeling
authors.forename: A
authors.initials: A
authors.lastname: Hochhaus
authors.forename: L
authors.initials: L
authors.lastname: Trahms
authors.forename: J H
authors.initials: JH
authors.lastname: Clement
mesh_terms: D001812:Blood-Brain Barrier / Q000166:cytology*; D042783:Endothelial Cells / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D060328:Magnetic Phenomena; D055806:Microvessels / Q000166:cytology*; D053758:Nanoparticles; D010316:Particle Size; D013057:Spectrum Analysis
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide