title: High-sensitivity chemiluminescent immunoassay investigation and application for the detection of T-2 toxin and major metabolite HT-2 toxin.
abstract: BACKGROUND
  T-2 toxin is a widely distributed mycotoxin in cereals. HT-2 toxin is the major metabolite, which is also a contaminant in cereals. T-2 toxin and HT-2 toxin have been identified as having carcinogenic, hepatotoxic, teratogenic and immunotoxic properties. To reduce the risk of contamination, a rapid, highly sensitive and inexpensive assay for the detection is required.
  
  
  RESULTS
  In this study a high-sensitivity chemiluminescent enzyme-linked immunoassay (CL-ELISA) of T-2 toxin and HT-2 toxin was developed. With the help of the chemiluminescent substrate, this protocol showed a highly sensitive character with an IC50as low as 33.28 ng mL-1and 27.27 ng mL-1for T-2 and HT-2, respectively. In addition, this method had no cross-reaction with other structurally related mycotoxins.
  
  
  CONCLUSIONS
  These results indicated that the developed CL-ELISA could be applied for the detection of T-2 toxin and HT-2 toxin in actual samples without complicated steps. © 2016 Society of Chemical Industry.
authors.affiliation: College of Life Science, Yantai University, Yantai 264005, P.R. China.
authors.forename: Yanshen
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Science, Yantai University, Yantai 264005, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Yantai University, Yantai 264005, P.R. China.|Hua Hai Property & Casualty Insurance Co., Ltd, Yantai, Shandong 264005, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Mao
authors.affiliation: College of Life Science, Yantai University, Yantai 264005, P.R. China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Science, Jining 272033, P.R. China.
authors.forename: Gongzhen
authors.initials: G
authors.lastname: Liu
authors.affiliation: College of Life Science, Yantai University, Yantai 264005, P.R. China.
authors.forename: Liting
authors.initials: L
authors.lastname: Song
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, P.R. China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Science, Yantai University, Yantai 264005, P.R. China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Life Science, Yantai University, Yantai 264005, P.R. China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: You
authors.affiliation: Logistics University Of People's Armed Police Forced Brigade Two, Tianjin 300000, P.R. China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Cao
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D002274:Carcinogens, Environmental / Q000032:analysis* / Q000378:metabolism; D002681:China; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements; D000432:Methanol / Q000737:chemistry; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D002274:Carcinogens, Environmental; D012997:Solvents; D013605:T-2 Toxin; C012351:HT-2 toxin; D000432:Methanol
keywords: CL-ELISA; HT-2 toxin; T-2 toxin; high-sensitivity; metabolite