title: Protective effect of Mimosa pudica L. in an L-arginine model of acute necrotising pancreatitis in rats.
abstract: Mimosa pudica is used in traditional medicine for treating various disorders such as inflammatory conditions, diarrhoea, insomnia, alopecia, urogenital infections and wounds. The present study investigated the effect of M. pudica extract (MPE) on L-arginine-induced acute necrotising pancreatitis in rats. The ethanolic extract of M. pudica leaves was studied for the presence of quercetin and gallic acid using high-performance liquid chromatography. Four groups were employed-normal control rats, L-arginine control rats (two intraperitoneal [i.p.] injections of 2 g/kg at an interval of 1 h), MPE-treated rats (400 mg/kg orally) and melatonin-treated rats (positive control 10 mg/kg i.p.), which were further divided into subgroups according to time points (24 h, 3 days and 14 days). Serum amylase, lipase, tumour necrosis factor-α (TNF-α), pancreatic amylase, nucleic acid content, protein, transforming growth factor-β1 (TGF-β1), thiobarbituric reactive substances, glutathione, nitrite/nitrate, collagen content and histopathological examination were carried out. MPE significantly improved acute necrotising pancreatitis by modulating diagnostic markers of pancreatitis such as serum lipase and pancreatic amylase, inflammation (TNF-α), and oxidative and nitrosative stress. Moreover, MPE administration induced regenerative changes in the pancreas evidenced by increased levels of pancreatic proteins, nucleic acid content and histopathology report. In addition, MPE improved TGF-β1 and collagen levels thereby preventing fibrosis. The current investigation indicates the novel role of MPE in reducing the severity of acute necrotising pancreatitis by plausible mechanisms such as anti-inflammatory and anti-fibrotic activity and by promoting repair and regeneration of the pancreas.
authors.affiliation: Department of RIC, IKG Punjab Technical University, Kapurthala, 144601, Punjab, India.|Sri Sai College of Pharmacy, Badhani, Pathankot, 145001, Punjab, India.
authors.forename: Jagdeep
authors.initials: J
authors.lastname: Kaur
authors.affiliation: Department of Life Sciences and Technology, Punjab Institute of Technology, IKG Punjab Technical University, Kapurthala, 144603, Punjab, India.
authors.forename: Shabir
authors.initials: S
authors.lastname: Sidhu
authors.affiliation: Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh, 160 014, India.
authors.forename: Kanwaljit
authors.initials: K
authors.lastname: Chopra
authors.affiliation: Sri Sai College of Pharmacy, Badhani, Pathankot, 145001, Punjab, India. masihkhan@gmail.com.
authors.forename: M U
authors.initials: MU
authors.lastname: Khan
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D031298:Mimosa; D019283:Pancreatitis, Acute Necrotizing / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001120:Arginine
keywords: L-arginine; Mimosa pudica; Pancreatitis; Regeneration