title: The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment.
abstract: In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSCs) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to non-textured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 h time points.
authors.affiliation: University of Nottingham, School of Pharmacy, Division of Drug Delivery and Tissue Engineering, Boots Science Building, University Park, Nottingham, NG7 2RD, UK. Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Technology İstanbul, 34668, Turkey.
authors.forename: Gökçen
authors.initials: G
authors.lastname: Yaşayan
authors.forename: Xuan
authors.initials: X
authors.lastname: Xue
authors.forename: Pamela
authors.initials: P
authors.lastname: Collier
authors.forename: Philip
authors.initials: P
authors.lastname: Clarke
authors.forename: Morgan R
authors.initials: MR
authors.lastname: Alexander
authors.forename: Maria
authors.initials: M
authors.lastname: Marlow
mesh_terms: D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D010051:Ovarian Neoplasms; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid