title: Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts.
abstract: Ellagic acid (EA), an antioxidant polyphenolic constituent of plant origin, has been reported to possess diverse pharmacological properties, including anti-inflammatory, anti-tumor and immunomodulatory activities. This work aimed to clarify the skin anti-photoaging properties of EA in human dermal fibroblasts. The skin anti-photoaging activity was evaluated by analyzing the reactive oxygen species (ROS), matrix metalloproteinase-2 (MMP-2), total glutathione (GSH) and superoxide dismutase (SOD) activity levels as well as cell viability in dermal fibroblasts under UV-B irradiation. When fibroblasts were exposed to EA prior to UV-B irradiation, EA suppressed UV-B-induced ROS and proMMP-2 elevation. However, EA restored total GSH and SOD activity levels diminished in fibroblasts under UV-B irradiation. EA had an up-regulating activity on the UV-B-reduced Nrf2 levels in fibroblasts. EA, at the concentrations used, was unable to interfere with cell viabilities in both non-irradiated and irradiated fibroblasts. In human dermal fibroblasts, EA plays a defensive role against UV-B-induced oxidative stress possibly through an Nrf2-dependent pathway, indicating that this compound has potential skin antiphotoaging properties.
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Beomyeol
authors.initials: B
authors.lastname: Baek
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Su Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Kim
authors.affiliation: Shebah Biotech Inc., Chuncheon 24398, Korea.
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Lim
authors.affiliation: Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
keywords: Ellagic acid; Glutathione; Superoxide dismutase; UV-B radiation