title: Ultrasensitive photoelectrochemical aptasensing of miR-155 using efficient and stable CH3NH3PbI3 quantum dots sensitized ZnO nanosheets as light harvester.
abstract: An ultrasensitive photoelectrochemical (PEC) aptasensor based on a novel signal amplification strategy was developed for the quantitative determination of microRNA (miR)-155. CH3NH3PbI3 quantum dots (QDs) functionalized ZnO nanosheets (NSs) were employed as the light harvester. Owing to the synergetic effect between CH3NH3PbI3 QDs and ZnO NSs, ZnO@CH3NH3PbI3 can provide an obviously increasing PEC signal by forming the heterojunction. Due to the larger steric hindrance, the sensitive decrease of the PEC signal can be achieved by the specific recognition between the primers and ssDNA of miR-155. In this sense, this developed aptasensor can achieve a high sensitivity (especially in the presence of the low concentrations of miR-155) and a wide detection range (0.01fmol/L to 20,000pmol/L). Under the optimal conditions, the proposed aptasensor offered an ultrasensitive and specific determination of miR-155 down to 0.005fmol/L. This aptassay method would open up a new promising platform at ultralow levels for early diagnose of different miRNA.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, Key Laboratory of Fluorine Chemistry and Chemical Materials of Shandong Province, University of Jinan, Jinan 250022, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.affiliation: Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Jianni
authors.initials: J
authors.lastname: Qi
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, Key Laboratory of Fluorine Chemistry and Chemical Materials of Shandong Province, University of Jinan, Jinan 250022, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, Key Laboratory of Fluorine Chemistry and Chemical Materials of Shandong Province, University of Jinan, Jinan 250022, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, Key Laboratory of Fluorine Chemistry and Chemical Materials of Shandong Province, University of Jinan, Jinan 250022, China.
authors.forename: Minhui
authors.initials: M
authors.lastname: Su
authors.affiliation: College of Pharmaceutical, Taishan Medical University, Tai'an 271016, China.
authors.forename: Baoxiu
authors.initials: B
authors.lastname: Jia
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, Key Laboratory of Fluorine Chemistry and Chemical Materials of Shandong Province, University of Jinan, Jinan 250022, China.
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, Key Laboratory of Fluorine Chemistry and Chemical Materials of Shandong Province, University of Jinan, Jinan 250022, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, Key Laboratory of Fluorine Chemistry and Chemical Materials of Shandong Province, University of Jinan, Jinan 250022, China. Electronic address: dubin61@gmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D006801:Humans; D008027:Light; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry / Q000648:ultrastructure; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D009942:Organometallic Compounds; D015034:Zinc Oxide
keywords: Heterojunction; MicroRNA; Nanosheet; PEC; Quantum dot; ZnO@CH(3)NH(3)PbI(3)