title: Competition between hydrogen bonding and protein aggregation in neuronal-like cells under exposure to 50 Hz magnetic field.
abstract: To investigate the role of hydrogen bonding and protein unfolding in human SH-SY5Y neuronal-like cells under exposure to a 50 Hz magnetic field (MF) at the intensity of 1 mT.
  
  
  
  Neuronal-like cells were placed into an incubator in a 5% CO2/95% air humidified at the temperature of 37.1 °C and exposed for 4 h to a 50 Hz MF at 1 mT. The exposure system consisted of two Helmholtz coils driven by AC voltage at 50 Hz. Exposed and control samples were studied using Fourier Transform Infrared (FTIR) Spectroscopy.
  
  
  
  The vibration bands of the methylene group increased significantly after 4 h of exposure. A significant shift to low energies of the Amide I band and an increase in the intensity of the parallel and antiparallel β-sheet structures with respect to the α-helix component were observed after exposure. The Amide II frequency did not change significantly whereas a relative increase of its integrated area with respect to Amide I mode occurred after exposure.
  
  
  
  These results can be explained assuming that both the mechanisms of protein aggregation as well as the increase in hydrogen bonding occurred in neuronal-like cells under exposure to a 50 Hz MF.
authors.affiliation: a Department of Mathematics and Computing, Physics and Earth Sciences , University of Messina , Messina , Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Calabrò
mesh_terms: D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D004560:Electricity; D006801:Humans; D006860:Hydrogen Bonding / Q000528:radiation effects*; D060526:Magnetic Fields; D009474:Neurons / Q000378:metabolism* / Q000528:radiation effects*; D066329:Protein Aggregates / Q000502:physiology / Q000528:radiation effects*; D058767:Protein Unfolding / Q000528:radiation effects*; D011829:Radiation Dosage
chemical_list: D066329:Protein Aggregates
keywords: FTIR spectroscopy; Magnetic field; SH-SY5Y neuronal-like cells; hydrogen bonding; protein aggregation