title: miR-132 targeting E2F5 suppresses cell proliferation, invasion, migration in ovarian cancer cells.
abstract: Accumulating evidence showed that microRNA-132 (miR-132) are involved in development and progression of several types of cancers, however, the function and underlying molecular mechanism of miR-132 in ovarian cancer remains unclear. In this study we investigated the biological roles and molecular mechanism of miR-132 in ovarian cancer. Here, we found that that the expression levels of miR-132 were dramatically decreased in ovarian cancer cell lines and clinical ovarian cancer tissue samples. Then, we found that introduction of miR-132 significantly suppressed the proliferation, colony formation, migration and invasion of ovarian cancer cells. Mechanism investigation revealed that miR-132 inhibited the expression of transcription factor E2F5 by specifically targeting its mRNA 3'UTR. Moreover, the expression level of E2F5 was significantly increased in ovarian cancer tissues than in the adjacent normal tissues, and its expression was inversely correlated with miR-132 expression in clinical ovarian cancer tissues. Additionally, silencing E2F5 was able to inhibit the proliferation, colony formation, migration and invasion of ovarian cancer cells, parallel to the effect of miR-132 overexpression on the ovarian cancer cells. Meanwhile, overexpression of E2F5 reversed the inhibition effect mediated by miR-132 overexpression. These results indicate that miR-132 suppresses the cell proliferation, invasion, migration in ovarian cancer cells by targeting E2F5.
authors.affiliation: Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510623, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Anesthesiology, Shanxi Cancer Hospital Taiyuan 030013, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Hou
authors.affiliation: Department of Pediatric Emergency, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510623, China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Xiong
authors.affiliation: Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510623, China.
authors.forename: Jun-Xiang
authors.initials: JX
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510623, China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510623, China.
authors.forename: Yong-Ying
authors.initials: YY
authors.lastname: Pan
authors.affiliation: Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510623, China.
authors.forename: Xing-Rong
authors.initials: XR
authors.lastname: Song
keywords: E2F5; Ovarian cancer; invasion; migration; proliferation