title: Genetic diversity of the captive Asian tapir population in Thailand, based on mitochondrial control region sequence data and the comparison of its nucleotide structure with Brazilian tapir.
abstract: The Asian tapir (Tapirus indicus) has been classified as Endangered on the IUCN Red List of Threatened Species (2008). Genetic diversity data provide important information for the management of captive breeding and conservation of this species. We analyzed mitochondrial control region (CR) sequences from 37 captive Asian tapirs in Thailand. Multiple alignments of the full-length CR sequences sized 1268 bp comprised three domains as described in other mammal species. Analysis of 16 parsimony-informative variable sites revealed 11 haplotypes. Furthermore, the phylogenetic analysis using median-joining network clearly showed three clades correlated with our earlier cytochrome b gene study in this endangered species. The repetitive motif is located between first and second conserved sequence blocks, similar to the Brazilian tapir. The highest polymorphic site was located in the extended termination associated sequences domain. The results could be applied for future genetic management based in captivity and wild that shows stable populations.
authors.affiliation: a Graduate School of Life Sciences , Ritsumeikan University , Kusatsu , Shiga Japan.|b The Graduate School, Kasetsart University, Jatujak , Bangkok , Thailand.|c Faculty of Veterinary Medicine , Kasetsart University , Nakhon Pathom , Thailand.
authors.forename: Yuttamol
authors.initials: Y
authors.lastname: Muangkram
authors.affiliation: a Graduate School of Life Sciences , Ritsumeikan University , Kusatsu , Shiga Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Amano
authors.affiliation: c Faculty of Veterinary Medicine , Kasetsart University , Nakhon Pathom , Thailand.
authors.forename: Worawidh
authors.initials: W
authors.lastname: Wajjwalku
authors.affiliation: c Faculty of Veterinary Medicine , Kasetsart University , Nakhon Pathom , Thailand.
authors.forename: Tanu
authors.initials: T
authors.lastname: Pinyopummintr
authors.affiliation: c Faculty of Veterinary Medicine , Kasetsart University , Nakhon Pathom , Thailand.
authors.forename: Nikorn
authors.initials: N
authors.lastname: Thongtip
authors.affiliation: c Faculty of Veterinary Medicine , Kasetsart University , Nakhon Pathom , Thailand.
authors.forename: Nongnid
authors.initials: N
authors.lastname: Kaolim
authors.affiliation: c Faculty of Veterinary Medicine , Kasetsart University , Nakhon Pathom , Thailand.
authors.forename: Manakorn
authors.initials: M
authors.lastname: Sukmak
authors.affiliation: d Bureau of Conservation, Research and Education , Zoological Park Organization, Dusit , Bangkok , Thailand.
authors.forename: Sumate
authors.initials: S
authors.lastname: Kamolnorranath
authors.affiliation: d Bureau of Conservation, Research and Education , Zoological Park Organization, Dusit , Bangkok , Thailand.
authors.forename: Boripat
authors.initials: B
authors.lastname: Siriaroonrat
authors.affiliation: d Bureau of Conservation, Research and Education , Zoological Park Organization, Dusit , Bangkok , Thailand.
authors.forename: Wanlaya
authors.initials: W
authors.lastname: Tipkantha
authors.affiliation: e Khao Kheow Open Zoo , Sriracha , Chonburi , Thailand.
authors.forename: Umaporn
authors.initials: U
authors.lastname: Maikaew
authors.affiliation: f Nakhon Ratchasima Zoo , Muang , Nakhon Ratchasima , Thailand.
authors.forename: Warisara
authors.initials: W
authors.lastname: Thomas
authors.affiliation: g Song Khla Zoo , Muang , Song Khla , Thailand.
authors.forename: Kanda
authors.initials: K
authors.lastname: Polsrila
authors.affiliation: h Chiang Mai Zoo , Muang , Chiang Mai , Thailand.
authors.forename: Kwanreaun
authors.initials: K
authors.lastname: Dongsaard
authors.affiliation: i Dusit Zoo, Dusit , Bangkok , Thailand.
authors.forename: Saowaphang
authors.initials: S
authors.lastname: Sanannu
authors.affiliation: j Safari World Public Company Limited , Klongsamwa, Bangkok , Thailand.
authors.forename: Anuwat
authors.initials: A
authors.lastname: Wattananorrasate
mesh_terms: D000818:Animals; D001938:Brazil; D004272:DNA, Mitochondrial / Q000235:genetics*; D056727:Endangered Species; D014644:Genetic Variation; D008928:Mitochondria / Q000235:genetics; D010527:Perissodactyla / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*; D013785:Thailand
chemical_list: D004272:DNA, Mitochondrial
keywords: D-loop; Malayan tapir; Tapirus indicus; genetic variation; mitochondrial DNA