title: Efficacy of solvent/detergent plasma after storage at 2-8 °C for 5 days in comparison to other plasma products to improve factor V levels in factor V deficient plasma.
abstract: OBJECTIVE
  Factor V (FV) plays an important role in coagulation. As no purified concentrate is available to restore critical FV levels, the main blood product used to replace FV is plasma. The aim of the present in vitro study was to compare the efficacy of the different available plasma products on the reversal of moderate and severe FV deficiency as assessed by ROTEM® and FV levels.
  
  
  METHODS
  Five different plasma products (6 batches of each) were compared to determine their effectiveness in replacing FV in plasma moderately or severely deficient in FV. Effectiveness was measured using the ROTEM® EXTEM clotting time (CT) and a factor V assay.
  
  
  RESULTS
  FFP, plasma frozen within 24 hours (FP24), Octaplas (solvent/detergent treated pooled plasma), as well as Octaplas and FP24 thawed and stored for 5 days (Octaplas TP and TP), were all used for in vitro replacement of FV. TP was significantly less effective at reversing a prolonged EXTEM CT and FV levels in FV deficient plasma than other tested products. There were no significant differences in EXTEM CT between Octaplas and Octaplas TP, while factor V activity was significantly lower in the Octaplas TP. There was no significant difference between Octaplas and FFP for EXTEM CT or FV activity.
  
  
  CONCLUSIONS
  Octaplas and Octaplas TP appear to have an equivalent ability to improve the EXTEM CT and could be considered as a treatment alternative to FFP in patients with FV deficiency.
authors.affiliation: Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Cushing
authors.affiliation: Unilabs, Coagulation Lab & Centre for Perioperative Thrombosis and Haemostasis, Zurich, Switzerland.
authors.forename: Lars
authors.initials: L
authors.lastname: Asmis
authors.affiliation: Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Carmencita
authors.initials: C
authors.lastname: Calabia
authors.affiliation: Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Jacob H
authors.initials: JH
authors.lastname: Rand
authors.affiliation: Department of Anesthesia, Zurich University Children's Hospital, Zurich, Switzerland. Electronic address: thorsten.haas@kispi.uzh.ch.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Haas
mesh_terms: D001793:Blood Preservation; D003902:Detergents / Q000737:chemistry*; D005165:Factor V / Q000737:chemistry / Q000378:metabolism*; D005166:Factor V Deficiency / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D010949:Plasma / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003902:Detergents; D005165:Factor V
keywords: Factor V; Fresh frozen plasma; Plasma; Thromboelastometry; Trauma