title: Metabolomic study of aging in mouse plasma by gas chromatography-mass spectrometry.
abstract: Metabolomic analysis of aging was performed in plasma samples of young (8 weeks) and old (72 weeks) mice as ethoxycarbonyl/methoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry (GC-MS). As new approaches, study of altered metabolism from aging was attempted by simultaneous profiling analysis of amino acids (AAs), organic acids (OAs) and fatty acids (FAs) by GC-MS in a single run combined with pattern analysis. As a result, 27 amino acids (AAs), 17 organic acids (OAs) and 24 fatty acids (FAs) were positively screened with large variations in plasma samples. Among altered metabolites, levels of six AAs (proline, methionine, 4-hydroxyproline, pipecolic acid, glutamic acid, α-aminoadipic acid) as neurotransmetters and nutrients, five OAs (2-hydroxybutyric acid, 2-hydroxyglutaric acid, cis-aconitic acid citric acid, isocitric acid) including intermediate metabolites in the TCA cycle, and three n-3 polyunsaturated FAs (PUFAs) of α-octadecatrienoic acid, eicosapentaenoic acid and docosahexaenoic acid as potential biomarkers were significantly different between young and old groups. Their levels were normalized to the corresponding mean values of the young group and then plotted into star symbol patterns, which were clearly distinct compared with numerical data and readily distinguishable for young and old groups. Thus, the present metabolomic screening and the star pattern recognition method might be useful for understanding the complexity of biochemical events in aging.
authors.affiliation: College of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Chan
authors.initials: C
authors.lastname: Seo
authors.affiliation: College of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Yun-Ho
authors.initials: YH
authors.lastname: Hwang
authors.affiliation: College of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Youngbae
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Center for Anti-Aging Industry, Pusan National University, Busan, Republic of Korea.
authors.forename: Bo Sun
authors.initials: BS
authors.lastname: Joo
authors.affiliation: College of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Yee
authors.affiliation: Center for Anti-Aging Industry, Pusan National University, Busan, Republic of Korea; Department of Biomedical Informatics, Pusan National University School of Medicine, Yangsan, Republic of Korea. Electronic address: Kimcm@pusan.ac.kr.
authors.forename: Cheol Min
authors.initials: CM
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Sunchon National University, Suncheon, Republic of Korea. Electronic address: paik815@sunchon.ac.kr.
authors.forename: Man-Jeong
authors.initials: MJ
authors.lastname: Paik
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000596:Amino Acids / Q000097:blood / Q000378:metabolism; D000818:Animals; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000596:Amino Acids; D005227:Fatty Acids
keywords: Aging; Amino acid; Fatty acid; Metabolomics; Organic acid