title: Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis.
abstract: Angiogenesis is a complex process of critical importance during development and in physiological and pathophysiological conditions. There is considerable research interest in studying the angiogenesis cascade and consequently a need for a physiologically valid, quantitative, and cost-effective assay. In this chapter, we describe the stem cell spheroid-based sprout assay in three-dimensional fibrin scaffold which allows fast and easy screening of pro- and anti-angiogenic effects of substances with a high degree of reproducibility.
authors.affiliation: Department of Physiology, Faculty of Medicine, Justus-Liebig University, Aulweg 129, 35392, Giessen, Germany.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Sharifpanah
authors.affiliation: Department of Physiology, Faculty of Medicine, Justus-Liebig University, Aulweg 129, 35392, Giessen, Germany. heinrich.sauer@physiologie.med.uni-giessen.de.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Sauer
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology*; D005337:Fibrin / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D054457:Tissue Scaffolds
chemical_list: D005337:Fibrin
keywords: Angiogenesis; Embryonic stem cells; Fibrin scaffold; In vitro model; Sprout assay; Three-dimensional culture