title: Low glucose dependent decrease of apoptosis and induction of autophagy in breast cancer MCF-7 cells.
abstract: Cancer cells have developed a number of adaptation mechanisms involving the signal activation of the transduction pathways, which promotes the progression and metastasis. Our results showed that the percentage of apoptotic MCF-7 cells incubated in the low glucose medium for 48 h was lower in comparison to those cultured in the high glucose medium, despite the high expression of the proapoptotic transcription factor-CHOP. Furthermore, the MCF-7 cells incubated in the low glucose medium for 48 h showed a higher expression of NF-κB p100/p52 subunits compared to cells incubated in the high glucose medium. Moreover, our findings demonstrated that the shortage of glucose strongly induces autophagy in MCF-7 cells. The activation of this process is not associated with the changes in the expression of mTOR kinase. We suggest, that the antiapoptotic chaperone ORP150 induction, transcription factor NF-κB2 activation, and increased autophagy constitute mechanisms protecting the MCF-7 cells against apoptosis.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Białystok, Mickiewicza 2A, 15-222, Białystok, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Krętowski
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Białystok, Mickiewicza 2A, 15-222, Białystok, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Borzym-Kluczyk
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Białystok, Mickiewicza 2A, 15-222, Białystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Stypułkowska
authors.affiliation: Department of Biology, Medical University of Białystok, Białystok, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Brańska-Januszewska
authors.affiliation: Department of Biology, Medical University of Białystok, Białystok, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Ostrowska
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Białystok, Mickiewicza 2A, 15-222, Białystok, Poland. mapasko@gmail.com.
authors.forename: Marzanna
authors.initials: M
authors.lastname: Cechowska-Pasko
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D052003:NF-kappa B p52 Subunit / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism
chemical_list: C497249:DDIT3 protein, human; D018840:HSP70 Heat-Shock Proteins; D052003:NF-kappa B p52 Subunit; D009363:Neoplasm Proteins; C050087:oxygen-regulated proteins; D051742:Transcription Factor CHOP; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D005947:Glucose
keywords: Apoptosis; Autophagy; CHOP; NF-κB2; ORP150; mTOR