title: Multimodal Nonlinear Optical Imaging of Live Cells Using Plasmon-Coupled DNA-Mediated Gold Nanoprism Assembly.
abstract: Multiphoton excitation microscopy techniques are the emerging nonlinear optical (NLO) imaging methods to watch the biological world due its ability to penetrate deep into living tissues. Driven by the need to develop multimodal NLO imaging probe, current article reports the design of DNA-mediated gold nanoprisms assembly based optical antennas to enhance multiphoton imaging capability in biological II window. Reported experimental data show a unique way to enhance second harmonic generation (SHG) and two-photon fluorescence (TPF) properties by several orders of magnitudes via plasmon coupled organization into gold nanoprism assembly structures. Experimental and theoretical modeling data using finite difference time domain (FDTD) simulations indicate that huge enhancement of SHG and TPF properties are mainly due to the electric quadrupole contribution and electric field enhancement. Using 1100 nm biological II window light, reported results demonstrated that antibody conjugated assembly structures are capable of exhibiting highly selective and very bright multimodal SHG and TPF imaging of human Hep G2 liver cancer cells.
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Sudarson Sekhar
authors.initials: SS
authors.lastname: Sinha
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Stacy
authors.initials: S
authors.lastname: Jones
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Teresa
authors.initials: T
authors.lastname: Demeritte
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Suhash Reddy
authors.initials: SR
authors.lastname: Chavva
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Jasmine
authors.initials: J
authors.lastname: Burrell
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Avijit
authors.initials: A
authors.lastname: Pramanik
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA;
authors.forename: Paresh Chandra
authors.initials: PC
authors.lastname: Ray