title: Antifungal Activity of Eucalyptus Oil against Rice Blast Fungi and the Possible Mechanism of Gene Expression Pattern.
abstract: Eucalyptus oil possesses a wide spectrum of biological activity, including anti-microbial, fungicidal, herbicidal, acaricidal and nematicidal properties. We studied anti-fungal activities of the leaf oil extracted from Eucalyptus. grandis × E. urophylla. Eleven plant pathogenic fungi were tested based on the mycelium growth rates with negative control. The results showed that Eucalyptus oil has broad-spectrum inhibitory effects toward these fungi. Remarkable morphological and structural alterations of hypha have been observed for Magnaporthe grisea after the treatment. The mRNA genome array of M. grisea was used to detect genes that were differentially expressed in the test strains treated by the Eucalyptus oil than the normal strains. The results showed 1919 genes were significantly affected, among which 1109 were down-regulated and 810 were up-regulated (p < 0.05, absolute fold change >2). According to gene ontology annotation analysis, these differentially expressed genes may cause abnormal structures and physiological function disorders, which may reduce the fungus growth. These results show the oil has potential for use in the biological control of plant disease as a green biopesticide.
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China. zhouzhou124@126.com.|College of Life Sciences and College of Resources, Sichuan Agricultural University, Ya'an 625000, China. zhouzhou124@126.com.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China. lmm201411@163.com.
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Li
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China. huanglijie1104@163.com.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Huang
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China. bioyang@163.com.
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Yang
authors.affiliation: College of Life Sciences and College of Resources, Sichuan Agricultural University, Ya'an 625000, China. roundtree318@hotmail.com.
authors.forename: Shu
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China. bailinhan@scu.edu.cn.
authors.forename: Lin-Han
authors.initials: LH
authors.lastname: Bai
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D005052:Eucalyptus / Q000737:chemistry*; D005656:Fungal Proteins / Q000096:biosynthesis; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D025301:Hyphae / Q000235:genetics / Q000254:growth & development; D020082:Magnaporthe / Q000187:drug effects* / Q000472:pathogenicity; D058977:Molecular Sequence Annotation; D025282:Mycelium / Q000235:genetics / Q000254:growth & development; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D010938:Plant Oils
keywords: E. grandis × E. urophylla oil; M. grisea; antifungal effects; biological control; mRNA Genome Array