title: Voltammetric analysis of Pinus needles with physiological, phylogenetic, and forensic applications.
abstract: Polyphenolic compounds are electrochemically active components of vegetal matter which were targeted under simple experimental conditions to produce voltammetric profiles characterizing the metabolite composition. Application to bivariate and multivariate chemometric techniques permits to discriminate the species and age of plant leaves, illustrated here for the case of six Pinus species from two different subgenera. Such responses, associated with the electrochemical oxidation of polyphenolic compounds (quercetin, gallic acid, ellagic acid, among others), define a voltammetric profile which varies systematically with the age of the leaves for the different species. The application of this methodology for phylogenetic studies, plant physiology, forensic science, and chemoecology is discussed. Graphical Abstract Image of Pinus in a typical Mediterranean forest; Courtesy of the Botanic Garden of the University of Valencia.
authors.affiliation: Institut de Restauració del Patrimoni, Universitat Politècnica de València, Camí de Vera 14, 46022, València, Spain.
authors.forename: Annette S
authors.initials: AS
authors.lastname: Ortiz-Miranda
authors.affiliation: Institut für Botanik und Landschaftsökologie, Botanischer Garten, Universität Greifswald, Soldmannstraße 15, 17489, Greifswald, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: König
authors.affiliation: Institut für Biochemie, Lehrstuhl für Analytische Chemie und Umweltchemie, Universität Greifswald, Felix-Hausdorff-Str. 4, 17487, Greifswald, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Kahlert
authors.affiliation: Institut für Biochemie, Lehrstuhl für Analytische Chemie und Umweltchemie, Universität Greifswald, Felix-Hausdorff-Str. 4, 17487, Greifswald, Germany.
authors.forename: Fritz
authors.initials: F
authors.lastname: Scholz
authors.affiliation: Institut de Restauració del Patrimoni, Universitat Politècnica de València, Camí de Vera 14, 46022, València, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Osete-Cortina
authors.affiliation: Institut de Restauració del Patrimoni, Universitat Politècnica de València, Camí de Vera 14, 46022, València, Spain.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Doménech-Carbó
authors.affiliation: Departament de Química Analítica, Universitat de València, Dr. Moliner 50, 46100, Burjassot València, Spain. antonio.domenech@uv.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Doménech-Carbó
mesh_terms: D003217:Conductometry / Q000379:methods*; D044707:Forensic Sciences / Q000379:methods*; D008422:Materials Testing; D028223:Pinus / Q000737:chemistry* / Q000145:classification* / Q000502:physiology; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry* / Q000145:classification / Q000502:physiology; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010936:Plant Extracts; D059808:Polyphenols
keywords: Chemoecology; Chemotaxonomy; Forensic sciences; Pinus species; Voltammetry