title: Pendrin, an anion exchanger on lung epithelial cells, could be a novel target for lipopolysaccharide-induced acute lung injury mice.
abstract: OBJECTIVE
  The aim of this study is to evaluate the role of pendrin in acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) and to explore whether pendrin expression existing on alveolar cells.
  
  
  METHODS
  ALI C57BL/6 mice model induced by lipopolysaccharide (LPS) was established. The expression of pendrin in lung was analyzed by RT-PCR and western blotting methods, the changes of lung inflammatory parameters and pathology were observed, the cellular distribution of pendrin in the lung was determined using immunofluorescence. Statistical comparisons between groups were made by two-tailed Student's t-test.
  
  
  RESULTS
  Enhanced expression of the slc26a4 gene and production of pendrin in lungs of LPS-induced ALI mice were confirmed. In comparison with vehicle-control mice, methazolamide treatment mitigated lung inflammatory parameters and pathology. IL-6 and MCP-1 in lung tissues and BALF in methazolamide-treated mice were statistically decreased. Methazolamide treatment had significant effect on the total protein concentration in the BALF and the ratio of lung wet/dry weight. The percentage of macrophages in the BALF was increased. There was a low expression of pendrin in ATII.
  
  
  CONCLUSIONS
  Pendrin may be involved in pathological process of LPS-induced ALI. Inhibition of the pendrin function could be used to treat ALI. Airway epithelial cell may be a valuable therapeutic target for discovering and developing new drugs and/or new therapeutic strategies for the treatment of ALI/ARDS.
authors.affiliation: Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Graduate School, Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100730, P. R. China.
authors.forename: Chun-E
authors.initials: CE
authors.lastname: Jia
authors.affiliation: Department of Respiratory and Critical Care Medicine, Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing Chao-Yang Hospital-Beijing Institute of Respiratory Medicine, Capital Medical University Beijing 100020, P. R. China.
authors.forename: Dingyuan
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Respiratory and Critical Care Medicine, Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing Chao-Yang Hospital-Beijing Institute of Respiratory Medicine, Capital Medical UniversityBeijing 100020, P. R. China; Department of Respiratory and Critical Care Medicine, China-Japan Friendship HospitalBeijing 100029, P. R. China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Dai
authors.affiliation: National Clinical Research Center of Respiratory DiseasesBeijing 100730, P. R. China; Department of Cell Biology, Institute of Geriatrics, Beijing HospitalBeijing 100730, P. R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Graduate School, Peking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730, P. R. China; Department of Respiratory and Critical Care Medicine, Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing Chao-Yang Hospital-Beijing Institute of Respiratory Medicine, Capital Medical UniversityBeijing 100020, P. R. China; Department of Respiratory and Critical Care Medicine, China-Japan Friendship HospitalBeijing 100029, P. R. China; National Clinical Research Center of Respiratory DiseasesBeijing 100730, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
keywords: ALI; ATII; LPS; Pendrin; airway epithelium; methazolamide