title: Acetonic Extract from the Feijoa sellowiana Berg. Fruit Exerts Antioxidant Properties and Modulates Disaccharidases Activities in Human Intestinal Epithelial Cells.
abstract: Feijoa sellowiana fruit has been shown to possess various biological activities, such as anti-bacterial and anti-cancer properties, in a variety of cellular models, but its activity on human intestinal epithelial cells has never been tested. The purpose of this study was to investigate the effects of the acetonic extract of F. sellowiana fruits on the viability, membrane peroxidation, disaccharidases activities and proliferation of in vitro models of human intestinal epithelial cells. To obtain this goal, Caco-2 and HT-29 cells were exposed to the acetonic extract for 24 h. Cell proliferation, viability, lactase and sucrase-isomaltase activity and H2 O2 -induced membrane lipid peroxidation were tested. We found that, compared to control conditions, the acetonic extract significantly increased lactase and sucrase-isomaltase activity in Caco-2, but not HT-29, cells, decreased proliferation, had no effects on viability and restored lipid peroxidation in both cell models. This study suggests that the acetonic extract improves lactase and sucrase-isomaltase activity, inhibits cell proliferation, have no cytotoxic effects and prevent lipid peroxidation of intestinal epithelial cells. These effects may be exploited in case of disaccharidases deficit and also as an adjuvant treatment of diseases related to oxidative stress. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Clinical and Experimental Medicine, Federico II University of Naples, Naples, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Turco
authors.affiliation: Department of Clinical and Experimental Medicine, Federico II University of Naples, Naples, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Palumbo
authors.affiliation: Department of Clinical and Experimental Medicine, Federico II University of Naples, Naples, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Andreozzi
authors.affiliation: Department of Clinical and Experimental Medicine, Federico II University of Naples, Naples, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sarnelli
authors.affiliation: Department of Biology, Federico II University of Naples, Naples, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: De Ruberto
authors.affiliation: Department of Physiology and Pharmacology 'Vittorio Erspamer', La Sapienza University of Rome, Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Esposito
authors.affiliation: Department of Biology, Federico II University of Naples, Naples, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Basile
authors.affiliation: Department of Clinical and Experimental Medicine, Federico II University of Naples, Naples, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Cuomo
mesh_terms: D000975:Antioxidants; D004186:Disaccharidases / Q000737:chemistry*; D004847:Epithelial Cells / Q000378:metabolism*; D031646:Feijoa / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D004186:Disaccharidases
keywords: disaccharidases deficiency; functional foods; lactose malabsorption; oxidative stress; sucrase-isomaltase deficiency