title: Structural characterization and in vitro antitumor activity of an acidic polysaccharide from Angelica sinensis (Oliv.) Diels.
abstract: A water-soluble polysaccharide extracted from the roots of Angelica sinensis (Oliv.) Diels, which is a traditional Chinese medicine herb, was fractioned and purified by Sephadex G-50 gel filtration chromatography. The structural characterization and antitumor activities of the purified polysaccharide fraction, named as ASP, were evaluated in the present study. ASP, which molecular weight was determined to be 80kDa by high-performance gel-permeation chromatography, is an acidic heteropolysaccharide consisting of glucuronic acid, glucose, arabinose and galactose in ratio of 1.00:1.70:1.85:5.02. It has a backbone composed of (1→3)-linked Galp, (1→6)-linked Galp and 2-OMe-(1→6)-linked Galp with three branches attached to O-3 of 2-OMe-(1→6)-linked Galp and terminated with GlcpA and Araf, and all of Araf and the majority of Glcp are distributed in branches. Moreover, all of GlcpA were presented as (1→)-linked GlcpA in branches. In in vitro antitumor assays, ASP displayed cytotoxicity against HepG2 cells (34.32±3.50% at the concentration of 1mg/mL) and MCF-7 cells (28.90±1.50% at the concentration of 1mg/mL) in a dose-dependent manner, and ASP also showed mild inhibitory activity against A549 cells.
authors.affiliation: Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, No. 1277, Jiefang Road, 430022 Wuhan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, No. 1277, Jiefang Road, 430022 Wuhan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Nature Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, No. 13, Hangkong Road, 430030 Wuhan, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Wang
authors.affiliation: Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, No. 1277, Jiefang Road, 430022 Wuhan, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Hubei Key Laboratory of Nature Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, No. 13, Hangkong Road, 430030 Wuhan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
authors.affiliation: Hubei Key Laboratory of Nature Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, No. 13, Hangkong Road, 430030 Wuhan, China. Electronic address: zhangwkp@163.com.
authors.forename: Kai-Ping
authors.initials: KP
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D029971:Angelica sinensis / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides
keywords: Acidic polysaccharide; Angelica sinensis; Anti-tumor; Structure