title: Inhibitory mechanism of butylated hydroxyanisole against infection of Fusarium proliferatum based on comparative proteomic analysis.
abstract: Fusarium proliferatum as a filamentous fungal pathogen can produce mycotoxins that can contaminate postharvest fruits and thus impact risks on human health. The extracellular proteomes of F. proliferatum grown in the absence and presence of butylated hydroxyanisole (BHA) were analyzed comparatively. A total of 66 significantly different expressed secreted proteins were identified by LC-ESI-MS/MS analysis. The BHA treatment suppressed the accumulation of some pathogenic factors such as aspartic protease, cell wall degradation enzymes, porin, superoxide dismutase and glyceraldehyde-3-phosphate dehydrogenase. On the contrary, the BHA treatment increased the abundances of some proteins, such as ATP binding cassette transporter substrate-binding protein and lipopolysaccharide-assembly lipoprotein, involved in the growth of F. proliferatum. These findings suggest that BHA treatment could influence the pathogenic ability of F. proliferatum via inhibiting the levels of virulence factors and cell wall degradation-associated enzymes. Moreover, the induction of the growth-related proteins after the BHA treatment suggests that the livelihood of F. proliferatum might depend on the cost of reduced pathogenic ability. This study has provided some evidence for understanding the complicated mechanisms of F. proliferatum infection in an effort to develop new targets for the control of this fungal pathogen.
  
  
  
  To better understand the inhibitory mechanism of F. proliferatum by butylated hydroxyanisole (BHA) treatment, a comprehensive proteomic analysis of the secreted proteins of F. proliferatum was firstly conducted. Among the 66 identified spots, 34 and 32 proteins were down- and up-accumulated significantly by BHA treatment, respectively. Many of the identified key protein species were involved in the pathogenic ability and the growth of F. proliferatum. This study is helpful for broadening our knowledge of the pathogenic mechanism of F. proliferatum.
authors.affiliation: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Qijie
authors.initials: Q
authors.lastname: Jian
authors.affiliation: Zhong Shan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gong
authors.affiliation: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Duan
authors.affiliation: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. Electronic address: ymjiang@scbg.ac.cn.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002853:Chromatography, Liquid; D005638:Fruit / Q000382:microbiology; D060585:Fusariosis / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*; D005670:Fusarium / Q000737:chemistry / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D010940:Plant Proteins / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry; D014774:Virulence / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D020543:Proteome; D002083:Butylated Hydroxyanisole
keywords: Antifungal agent; Butylated hydroxyanisole; Fusarium proliferatum; Pathogenic ability; Proteomic; Secretome