title: The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.
abstract: Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatment of human diseases. Recombinant production by the baculovirus/insect cell expression system is particularly useful for expressing proteins of eukaryotic origin and their complexes. MultiBac, an advanced baculovirus/insect cell system, has been widely adopted in the last decade to produce multiprotein complexes with many subunits that were hitherto inaccessible, for academic and industrial research and development. The MultiBac system, its development and numerous applications are presented. Future opportunities for utilizing MultiBac to catalyze discovery are outlined.
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.|Unit of Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, UMI 3265, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.
authors.forename: Duygu
authors.initials: D
authors.lastname: Sari
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.|Unit of Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, UMI 3265, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.
authors.forename: Kapil
authors.initials: K
authors.lastname: Gupta
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.|Unit of Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, UMI 3265, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.
authors.forename: Deepak Balaji
authors.initials: DB
authors.lastname: Thimiri Govinda Raj
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.|Unit of Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, UMI 3265, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Aubert
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.|Unit of Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, UMI 3265, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.
authors.forename: Petra
authors.initials: P
authors.lastname: Drncová
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.|Unit of Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, UMI 3265, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Garzoni
authors.affiliation: Geneva Biotech SARL, Avenue de la Roseraie 64, 1205, Genève, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fitzgerald
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France. iberger@embl.fr.|Unit of Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, UMI 3265, 71 avenue des Martyrs, 38042, Grenoble Cedex 9, France. iberger@embl.fr.|School of Biochemistry, University of Bristol, Bristol, BS8 1TD, UK. iberger@embl.fr.
authors.forename: Imre
authors.initials: I
authors.lastname: Berger
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D030562:Databases, Protein; D055808:Drug Discovery / Q000379:methods; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006801:Humans; D008958:Models, Molecular; D046912:Multiprotein Complexes; D015202:Protein Engineering / Q000379:methods*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D011994:Recombinant Proteins; D014764:Viral Proteins
keywords: Baculovirus; Electron microscopy; Gene transfer; Insect cell expression system; Lepidoptera; MultiBac; Protein complexes; Structural biology; Synthetic biology; Vaccines; Virus-like particles (VLPs); X-ray crystallography