title: Assessment of NK Cell Metabolism.
abstract: There has been increasing recognition of the importance of metabolism on immune cell differentiation, homeostasis, and function. Recently, our lab and others have begun to investigate the metabolic requirements for NK cell differentiation and activation. Here, we describe approaches for the in vitro assessment of NK cell metabolism. We present methods for using inhibitors to alter cellular metabolism, measurement of intracellular ATP in NK cells, assessment of real-time glycolysis and oxidative phosphorylation by an extracellular flux assay from Seahorse Biosciences, and some basic protocols for stimulation of NK cells via cytokines and receptors.
authors.affiliation: Division of Rheumatology, Department of Pediatrics, Washington University School of Medicine, 660 S. Euclid Avenue, Box 8208, St. Louis, MO, 63110, USA.
authors.forename: Molly P
authors.initials: MP
authors.lastname: Keppel
authors.affiliation: Division of Rheumatology, Department of Pediatrics, Washington University School of Medicine, 660 S. Euclid Avenue, Box 8208, St. Louis, MO, 63110, USA. cooper_m@kids.wustl.edu.
authors.forename: Megan A
authors.initials: MA
authors.lastname: Cooper
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D006019:Glycolysis; D006706:Homeostasis; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D010085:Oxidative Phosphorylation
chemical_list: D016207:Cytokines; D000255:Adenosine Triphosphate
keywords: Glycolysis; Interferon-gamma; Metabolism; Natural killer cells; Oxidative phosphorylation