title: Making Bunyaviruses Talk: Interrogation Tactics to Identify Host Factors Required for Infection.
abstract: The identification of host cellular genes that act as either proviral or antiviral factors has been aided by the development of an increasingly large number of high-throughput screening approaches. Here, we review recent advances in which these new technologies have been used to interrogate host genes for the ability to impact bunyavirus infection, both in terms of technical advances as well as a summary of biological insights gained from these studies.
authors.affiliation: Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. ariblett@mail.med.upenn.edu.
authors.forename: Amber M
authors.initials: AM
authors.lastname: Riblett
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. domsr@email.chop.edu.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Doms
mesh_terms: D002044:Bunyaviridae Infections / Q000276:immunology* / Q000473:pathology*; D005820:Genetic Testing / Q000379:methods; D057166:High-Throughput Screening Assays; D054884:Host-Pathogen Interactions; D006801:Humans; D016851:Orthobunyavirus / Q000472:pathogenicity*
keywords: RNAi screening; affinity purification mass spectrometry; bunyavirus; haploid genetic screening; high-throughput screening; host-pathogen interaction; yeast two-hybrid