title: Kudiezi Injection(®) Alleviates Blood-Brain Barrier Disruption After Ischemia-Reperfusion in Rats.
abstract: This study was designed to examine the effect of KDZ, on the BBB disruption in rat underwent MCAO and reperfusion.
  
  
  
  Male Sprague-Dawley rats (260-280 g) were subjected to 60 minutes MCAO followed by reperfusion. KDZ (4 mL/kg) was administrated before ischemia. The Evans blue extravasation, albumin leakage, brain water content, TJ proteins, caveolin-1, p-caveolin-1, Src, and p-Src were evaluated. Neurological scores, cerebral infarction, and CBF were assessed. The binding affinity of KDZ to Src was examined.
  
  
  
  I/R evoked a range of insults including Evans blue extravasation, albumin leakage, brain water content increase, CBF decrease, cerebral infarction, and neurological deficits, all of which were attenuated by KDZ. Meanwhile, KDZ inhibited TJ proteins down-expression, expression of caveolin-1, phosphorylation of caveolin-1 and Src after I/R. In addition, SPR revealed binding of KDZ to Src with high affinity.
  
  
  
  KDZ protects BBB from disruption and improves cerebral outcomes following I/R via preventing the degradation of TJ proteins, caveolin-1 expression, and inhibiting p-caveolin-1 and p-Src, which were most likely attributable to the ability of its main ingredients to bind to Src and inhibit its phosphorylation.
authors.affiliation: Department of Traditional Chinese Medicine, Peking University People's Hospital, Beijing, China.
authors.forename: Fu-Qin
authors.initials: FQ
authors.lastname: Chen
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Chun-Shui
authors.initials: CS
authors.lastname: Pan
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Mao
authors.affiliation: Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.
authors.forename: Hong-Na
authors.initials: HN
authors.lastname: Mu
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Wang
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Chuan-She
authors.initials: CS
authors.lastname: Wang
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Fan
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Yuan-Chen
authors.initials: YC
authors.lastname: Cui
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Yun-Pei
authors.initials: YP
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, Peking University People's Hospital, Beijing, China.
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Department of Traditional Chinese Medicine, Peking University People's Hospital, Beijing, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Bai
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.|Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.|Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.|Beijing microvascular institute of integration of Chinese and western medicine, Beijing, China.
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Han
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000473:pathology*; D051242:Caveolin 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000473:pathology* / Q000517:prevention & control; D062725:Tight Junction Proteins / Q000187:drug effects; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D062725:Tight Junction Proteins; D019061:src-Family Kinases
keywords: Ixeris sonchifolia Hance; Src; albumin leakage; caveolin-1; tight junction proteins