title: Genetic Diversity of Artybash Virus in the Laxmann's Shrew (Sorex caecutiens).
abstract: Although based on very limited M and L segment sequences, Artybash virus (ARTV) was proposed previously as a unique hantavirus harbored by the Laxmann's shrew (Sorex caecutiens). To verify this conjecture, lung tissues from 68 Laxmann's shrews, captured during 2006 to 2014 in eastern Siberia, Russia, and Hokkaido, Japan, were analyzed for ARTV RNA using reverse transcription polymerase chain reaction (RT-PCR). ARTV RNA was detected in six Laxmann's shrews. Pairwise alignment and comparison of partial- and full-length S, M, and L segment sequences from these Laxmann's shrews, as well as phylogenetic analyses, using maximum likelihood and Bayesian methods indicated that ARTV was distinct from other soricine shrew-borne hantaviruses and representative hantaviruses harbored by rodents, moles, and bats. Taxonomic identity of the ARTV-infected Laxmann's shrews was confirmed by full-length cytochrome b mitochondrial DNA sequence analysis. Our data indicate that the hantavirus previously known as Amga virus (MGAV) represents genetic variants of ARTV. Thus, the previously proposed designation of ARTV/MGAV should be replaced by ARTV.
authors.affiliation: 1 Infectious Disease Surveillance Center, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Satoru
authors.initials: S
authors.lastname: Arai
authors.affiliation: 2 Department of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, University of Hawaii at Manoa , Honolulu, Hawaii.
authors.forename: Hae Ji
authors.initials: HJ
authors.lastname: Kang
authors.affiliation: 2 Department of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, University of Hawaii at Manoa , Honolulu, Hawaii.
authors.forename: Se Hun
authors.initials: SH
authors.lastname: Gu
authors.affiliation: 3 Institute of Low Temperature Science, Hokkaido University , Sapporo, Japan .
authors.forename: Satoshi D
authors.initials: SD
authors.lastname: Ohdachi
authors.affiliation: 4 Department of Biology and Museum of Southwestern Biology, University of New Mexico , Albuquerque, New Mexico .
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Cook
authors.affiliation: 5 State Research Center of Virology and Biotechnology "Vector," Koltsovo , Russia .
authors.forename: Liudmila N
authors.initials: LN
authors.lastname: Yashina
authors.affiliation: 1 Infectious Disease Surveillance Center, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Keiko
authors.initials: K
authors.lastname: Tanaka-Taya
authors.affiliation: 6 Institute of Systematics and Ecology of Animals , Novosibirsk, Russia .
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Abramov
authors.affiliation: 7 Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan .
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.affiliation: 1 Infectious Disease Surveillance Center, National Institute of Infectious Diseases , Tokyo, Japan .|8 Kawasaki City Institute for Public Health , Kanagawa, Japan .
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okabe
authors.affiliation: 1 Infectious Disease Surveillance Center, National Institute of Infectious Diseases , Tokyo, Japan .
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
authors.affiliation: 2 Department of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, University of Hawaii at Manoa , Honolulu, Hawaii.
authors.forename: Richard
authors.initials: R
authors.lastname: Yanagihara
mesh_terms: D000818:Animals; D014644:Genetic Variation; D006476:Orthohantavirus / Q000235:genetics* / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000302:isolation & purification*; D012426:Russia; D012788:Shrews / Q000821:virology*
chemical_list: D012367:RNA, Viral
keywords: Hantavirus; Japan; RT-PCR; Russia; Shrew.