title: Recombinant Newcastle disease virus (NDV/Anh-IL-2) expressing human IL-2 as a potential candidate for suppresses growth of hepatoma therapy.
abstract: Newcastle disease virus (NDV) have shown oncolytic therapeutic efficacy in preclinical study and are currently approved for clinical trials. NDV Anhinga strain which is a mesogenic strain should be classified as lytic strain and has a therapeutic efficacy in hepatocellular cancer. In this study, we evaluated the capacity of NDV Anhinga strain to elicit immune reaction in vivo and the possibility for using as a vaccine vector for expressing tumor therapeutic factors. Interleukin-2 (IL-2) could boost the immune response against the tumor cells. Therefore, we use NDV Anhinga strain as backbone to construct a recombinant virus (NDV/Anh-IL-2) expressing IL-2. The virus growth curve showed that the production of recombinant NDV/Anh-IL-2 was slightly delayed compared to the wild type. The NDV/Anh-IL-2 strain could express soluble IL-2 and effectively inhibit the growth of hepatocellular carcinoma in vivo. 60 days post-treatment, mice which were completely cured by previous treatment were well protected when rechallenged with the same tumor cell. From the H&E-stained sections, intense infiltration of lymphocyte was observed in the NDV Anhinga strain treated group, especially in NDV/Anh-IL-2 group. The NDV Anhinga strain could not only kill the tumor directly, but could also elicit immune reaction and a potent immunological memory when killing tumor in vivo. In conclusion, the Anhinga strain could be an effective vector for tumor therapy; the recombinant NDV/Anh-IL-2 strain expressing soluble IL-2 is a promising candidate for hepatoma therapy.
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China.
authors.forename: Jinjiao
authors.initials: J
authors.lastname: He
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: An
authors.affiliation: Department of Stomach, Spleen and Portal Hypertension, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Str, Nangang District, Harbin, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China.
authors.forename: Yunye
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Yan
authors.affiliation: School of Life Science, Henan Normal University, 46 Jianshe Road E., Xinxiang, PR China.
authors.forename: Xianlong
authors.initials: X
authors.lastname: Ye
authors.affiliation: School of Life Science, Henan Normal University, 46 Jianshe Road E., Xinxiang, PR China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Qi
authors.affiliation: School of Food Science and Technology, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China.
authors.forename: Shenglong
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China.
authors.forename: Jiechao
authors.initials: J
authors.lastname: Yin
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China. Electronic address: deshangli@163.com.
authors.forename: Deshan
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Life Science, Northeast Agriculture University, Mucai Street 59, Xiangfang District, Harbin, PR China. Electronic address: wangwenfei@neau.edu.cn.
authors.forename: Wenfei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D019496:Cancer Vaccines; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000628:therapy*; D002460:Cell Line; D045744:Cell Line, Tumor; D002645:Chickens; D006224:Cricetinae; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D051379:Mice; D009522:Newcastle disease virus / Q000235:genetics*; D047368:Tumor Burden
chemical_list: D019496:Cancer Vaccines; C508594:IL2 protein, human; D007376:Interleukin-2
keywords: Hepatocellular carcinoma; Hepatoma; IL-2; Newcastle disease virus; Oncolysis