title: Longitudinal study of experimental induction of AA amyloidosis in mice seeded with homologous and heterologous AA fibrils.
abstract: OBJECTIVE
  To investigate pathogenesis and kinetics of experimentally induced murine AA amyloidosis seeded with homologous (murine) and heterologous (bovine) AA fibrils.
  
  
  METHODS
  Experimental AA amyloidosis was induced by administration of inflammatory stimulus and preformed AA fibrils to a total of 111 female C57/Black mice. In this longitudinal study, heterologous (bovine) as well as homologous (murine) AA fibrils were injected intraperitoneally to mice in various combinations. Re-stimulation was done at 120 or 300 days post first inoculation. To analyze the intensity of amyloid depositions in mice organs, immunohistochemical techniques and image J software were used. Assessment of cytokines level in sera was done using a Mouse Th1/Th2/Th17 Cytokine CBA Kit.
  
  
  RESULTS
  Incidence and severity of AA amyloidosis were quite low in mice inoculated with heterologous bovine AA fibrils than homologous murine one. Homologous AA fibrils administration at first and second inoculation caused maximum amount of amyloid depositions and severe systemic form of amyloidosis. Increase in the level of pro-inflammatory cytokine IL-6 was observed after first inoculation, while second inoculation caused a further increase in the level of anti-inflammatory cytokine IL-10.
  
  
  CONCLUSIONS
  AA amyloidosis can be induced by heterologous as well as homologous AA fibrils. Severity of AA amyloidosis induced with homologous AA fibrils is higher compared to heterologous AA fibrils.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, Gifu, 501-1193, Japan.|Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Naeem
authors.initials: N
authors.lastname: Muhammad
authors.affiliation: Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Murakami
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, Gifu, 501-1193, Japan.|Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Inoshima
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, Gifu, 501-1193, Japan. ishiguna@gifu-u.ac.jp.|Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan. ishiguna@gifu-u.ac.jp.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Ishiguro
mesh_terms: D064591:Allografts; D000682:Amyloid / Q000378:metabolism*; D000686:Amyloidosis / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000097:blood; D005260:Female; D064593:Heterografts; D007668:Kidney / Q000378:metabolism; D008810:Mice, Inbred C57BL; D012835:Silver Nitrate / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism
chemical_list: D000682:Amyloid; D016207:Cytokines; D012835:Silver Nitrate
keywords: AA fibrils; Amyloidosis; Cytokine; Heterologous; Homologous; SAA