title: Developmental toxicity of bisphenol A diglycidyl ether (epoxide resin badge) during the early life cycle of a native amphibian species.
abstract: Bisphenol A diglycidyl ether (BADGE) is used in packaging materials, in epoxy adhesives, and as an additive for plastics, but it is also a potential industrial wastewater contaminant. The aim of the present study was to evaluate the adverse effects of BADGE on Rhinella arenarum by means of standardized bioassays at embryo-larval development. The results showed that BADGE was more toxic to embryos than to larvae at all exposure times. At acute exposure, lethality rates of embryos exposed to concentrations of 0.0005 mg/L BADGE and greater were significantly higher than rates in the vehicle control, whereas lethality rates of larvae were significantly higher in concentrations of 10 mg/L BADGE and greater. The toxicity then increased significantly, with 96-h median lethal concentrations (LC50s) of 0.13 mg/L and 6.9 mg/L BADGE for embryos and larvae, respectively. By the end of the chronic period, the 336-h LC50s were 0.04 mg/L and 2.2 mg/L BADGE for embryos and larvae, respectively. This differential sensitivity was also ascertained by the 24-h pulse exposure experiments, in which embryos showed a stage-dependent toxicity, with blastula being the most sensitive stage and S.23 the most resistant. The most important sublethal effects in embryos were cell dissociation and delayed development, whereas the main abnormalities observed in larvae related to neurotoxicity, as scare response to stimuli and narcotic effect. Environ Toxicol Chem 2016;35:3031-3038. © 2016 SETAC.
authors.affiliation: Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
authors.forename: Ianina
authors.initials: I
authors.lastname: Hutler Wolkowicz
authors.affiliation: Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
authors.forename: Gabriela V
authors.initials: GV
authors.lastname: Svartz
authors.affiliation: Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
authors.forename: Carolina M
authors.initials: CM
authors.lastname: Aronzon
authors.affiliation: Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
authors.forename: Christina
authors.initials: C
authors.lastname: Pérez Coll
mesh_terms: D000818:Animals; D001001:Anura / Q000254:growth & development / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D004852:Epoxy Compounds / Q000633:toxicity*; D004853:Epoxy Resins / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D007928:Lethal Dose 50; D008018:Life Cycle Stages / Q000187:drug effects*; D008853:Microscopy; D018675:Toxicity Tests; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004852:Epoxy Compounds; D004853:Epoxy Resins; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds
keywords: Bisphenol A diglycidyl ether; Neurotoxicity; Stage-dependent toxicity; Standardized toxicity bioassays; Teratogenesis