title: Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
abstract: The fission yeast model system Schizosaccharomyces pombe is used to study fundamental biological processes. To continue to fill gaps in the Sz. pombe gene deletion collection, we constructed a set of 90 haploid gene deletion strains covering many previously uncharacterized genes. To begin to understand the function of these genes, we exposed this collection of strains to a battery of stress conditions. Using this information in combination with microscopy, proteomics and mini-chromosome loss assays, we identified genes involved in cell wall integrity, cytokinesis, chromosome segregation and DNA metabolism. This subset of non-essential gene deletions will add to the toolkits available for the study of biological processes in Sz. pombe. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Chen
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Janel R
authors.initials: JR
authors.lastname: Beckley
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Liping
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Feoktistova
authors.affiliation: Genome Technology Center, Stanford University School of Medicine, Palo Alto, CA, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Jensen
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Rhind
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Gould
mesh_terms: D002455:Cell Division / Q000502:physiology*; D002473:Cell Wall / Q000502:physiology*; D015825:Chromosomes, Fungal / Q000502:physiology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029702:Schizosaccharomyces pombe Proteins
keywords: DNA metabolism; Schizosaccharomyces pombe; cytokinesis; fission yeast; gene deletions; stress sensitivities