title: In Vitro Antiviral Activity of Germacrone Against Porcine Reproductive and Respiratory Syndrome Virus.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is one of the most serious diseases affecting the swine industry worldwide; however, there is no efficient control strategies against PRRSV at present. Therefore, development of new antiviral treatment strategies is urgently needed. As reported, germacrone can efficiently impair influenza virus replication. In this study, we exploited whether germacrone has the potential to inhibit PRRSV infection. Our results showed that the germacrone significantly inhibited replication of PRRSV in vitro and repressed the synthesis of viral RNA and protein. However, it did not block PRRSV binding and entry. Further studies confirmed that germacrone impaired PRRSV replication at an early stage, and inhibited infection of both classic and highly pathogenic type II PRRSV strains. Collectively, our findings imply that the germacrone has the potential to be used as an anti-PRRSV drug.
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Jiaping
authors.initials: J
authors.lastname: Feng
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Bai
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Cui
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Han
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Jiexiong
authors.initials: J
authors.lastname: Xie
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Shi
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China. guihongzh@scau.edu.cn.|Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. guihongzh@scau.edu.cn.
authors.forename: Guihong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000188:drug therapy / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000502:physiology; D045788:Sesquiterpenes, Germacrane / Q000494:pharmacology*; D013552:Swine; D053586:Virus Internalization / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D045788:Sesquiterpenes, Germacrane; C048393:germacrone