title: Isolation and characterization of atrazine-degrading strain Shewanella sp. YJY4 from cornfield soil.
abstract: Atrazine has been used worldwide for over 50 years as a chemical herbicide. A strain of bacteria, YJY4 which utilizes atrazine as its sole nitrogen source for growth was isolated from agricultural black soil in northeastern China. 16S rDNA sequencing identified YJY4 as a Shewanella sp. PCR analysis and sequencing confirmed that YJY4 contained atrazine-degrading atzA, atzB and atzC genes. These genes revealed high similarity with those in Pseudomonas sp. ADP and Arthrobacter sp. TC1. The strain YJY4 was observed to degrade atrazine (100 mg l(-1) ) to cyanuric acid completely after 36 h. To the best of our knowledge, YJY4 was the first reported Shewanella sp. to grow in pure culture with atrazine serving as a sole source of nitrogen. Therefore, YJY4 may help with atrazine biodegradation and may become an abundant resource of atrazine degradation strains.
  
  
  CONCLUSIONS
  A new isolated strain, YJY4 showed high atrazine-degrading ability, being able to degrade 100 mg l(-1) atrazine completely in 36 h. Strain YJY4 was identified as Shewanella sp. and contained atrazine-degrading atzA, atzB and atzC genes. This study examined the degradation mechanism and metabolic ability of this strain and for the bioremediation of contaminated environments, provides more strain selection and determines the strain of atrazine bioremediation potential.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Ye
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: J B
authors.initials: JB
authors.lastname: Zhang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Gao
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: H T
authors.initials: HT
authors.lastname: Li
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: D
authors.initials: D
authors.lastname: Liang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: R M
authors.initials: RM
authors.lastname: Liu
mesh_terms: D001173:Arthrobacter / Q000235:genetics; D001280:Atrazine / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics; D006540:Herbicides / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics; D020592:Shewanella / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012987:Soil; D012988:Soil Microbiology; D014227:Triazines / Q000378:metabolism
chemical_list: D004275:DNA, Ribosomal; D006540:Herbicides; D012987:Soil; D014227:Triazines; C004632:cyanuric acid; D009584:Nitrogen; D001280:Atrazine
keywords: Shewanella sp.; atrazine; biodegradation; identify; metabolic ability