title: Detection and isolation of Bluetongue virus from commercial vaccine batches.
abstract: In this report we describe the detection and identification of Bluetongue virus (BTV) contaminations in commercial vaccines. BTV RNA was detected in vaccine batches of Lumpy skin disease (LSD) and Sheep pox (SP) using quantitative PCR (qPCR) for VP1 and NS3 genes. Both batches were positive for VP1 and NS3 in qPCR. The LSD vaccine-derived sample was positive for VP1 and VP2 in conventional PCR. The SP vaccine-derived sample was examined by amplification of VP1, VP4, VP6, VP7, NS2 and NS3 gene segments in conventional PCR. The SP vaccine-derived sample was further propagated in embryonated chicken eggs (ECE) and Vero cells. Preliminary sequence analysis showed that the LSD vaccine-derived sequence was 98-99% similar to BTV9. Analysis of the six genomic segments from the SP vaccine-derived isolate showed the highest similarity to BTV26 (66.3-97.8%). These findings are particularly important due to the effect of BTV on cattle and sheep, for which the vaccines are intended. They also demonstrate the necessity of rigorous vaccine inspection and strict vaccine production control.
authors.affiliation: Division of Virology, Kimron Veterinary Institute, Bet Dagan, PO Box 12, 50250, Israel.
authors.forename: Velizar
authors.initials: V
authors.lastname: Bumbarov
authors.affiliation: Division of Virology, Kimron Veterinary Institute, Bet Dagan, PO Box 12, 50250, Israel.
authors.forename: Natalia
authors.initials: N
authors.lastname: Golender
authors.affiliation: Division of Virology, Kimron Veterinary Institute, Bet Dagan, PO Box 12, 50250, Israel. Electronic address: orane@moag.gov.il.
authors.forename: Oran
authors.initials: O
authors.lastname: Erster
authors.affiliation: Division of Virology, Kimron Veterinary Institute, Bet Dagan, PO Box 12, 50250, Israel.
authors.forename: Yevgeny
authors.initials: Y
authors.lastname: Khinich
mesh_terms: D000818:Animals; D001820:Bluetongue virus / Q000302:isolation & purification*; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004340:Drug Contamination; D005814:Genes, Viral; D060888:Real-Time Polymerase Chain Reaction; D014709:Vero Cells; D014765:Viral Vaccines / Q000032:analysis*
chemical_list: D014765:Viral Vaccines
keywords: Bluetongue virus; NS3; Real-time PCR; VP1; Vaccine safety