title: Flyception: imaging brain activity in freely walking fruit flies.
abstract: Genetically encoded calcium sensors have enabled monitoring of neural activity in vivo using optical imaging techniques. Linking neural activity to complex behavior remains challenging, however, as most imaging systems require tethering the animal, which can impact the animal's behavioral repertoire. Here, we report a method for monitoring the brain activity of untethered, freely walking Drosophila melanogaster during sensorially and socially evoked behaviors to facilitate the study of neural mechanisms that underlie naturalistic behaviors.
authors.affiliation: Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, California, USA.
authors.forename: Dhruv
authors.initials: D
authors.lastname: Grover
authors.affiliation: Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, California, USA.
authors.forename: Takeo
authors.initials: T
authors.lastname: Katsuki
authors.affiliation: Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, California, USA.|Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.|Department of Cognitive Science, University of California, San Diego, La Jolla, California, USA.
authors.forename: Ralph J
authors.initials: RJ
authors.lastname: Greenspan
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D004331:Drosophila melanogaster / Q000502:physiology*; D005456:Fluorescent Dyes; D009424:Nervous System Physiological Phenomena; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D061848:Optical Imaging / Q000379:methods*; D016138:Walking / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium