title: Analysis of lysosomal membrane proteins exposed to melanin in HeLa cells.
abstract: OBJECTIVE
  There have been developed to use targeting ability for antimicrobial, anticancerous, gene therapy and cosmetics through analysis of various membrane proteins isolated from cell organelles.
  
  
  METHODS
  It was examined about the lysosomal membrane protein extracted from lysosome isolated from HeLa cell treated by 100 ppm melanin for 24 hours in order to find associated with targeting ability to melanin using by 2-dimensional electrophoresis.
  
  
  RESULTS
  The result showed 14 up-regulated (1.5-fold) and 13 down-regulated (2.0-fold) spots in relation to melanin exposure.
  
  
  CONCLUSIONS
  It has been found that lysosomal membrane proteins are associated with melanin to decolorize and quantity through cellular activation of lysosome.
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea.
authors.forename: Seung Hyuck
authors.identifier: 0000-0002-9023-6812
authors.initials: SH
authors.lastname: Bang
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea.
authors.forename: Dong Jun
authors.identifier: 0000-0002-4209-0302
authors.initials: DJ
authors.lastname: Park
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, Korea.
authors.forename: Yang-Hoon
authors.identifier: 0000-0002-3406-4868
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea.
authors.forename: Jiho
authors.identifier: 0000-0001-6025-7746
authors.initials: J
authors.lastname: Min
keywords: 2-Dimensional electrophoresis; HeLa cell; Lysosomal membrane proteins; Lysosome; Melanin