title: Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep.
abstract: OBJECTIVE
  This study was aimed to detect ovine pulmonary adenocarcinoma (OPA) in sheep flocks affected with pulmonary disorders at organized farm.
  
  
  METHODS
  A total of 75 sheep died naturally were thoroughly examined for the lesions of OPA during necropsy. Tissue sections from affected portion of the lungs from each animal were collected aseptically and divided into two parts; one each for polymerase chain reaction (PCR) and another for histopathology.
  
  
  RESULTS
  On PCR examination of lung tissues, six sheep (8%) were found to be positive for JSRV. Two of them were 3-6 months of age and did not show clinical signs/gross lesions of OPA. Four adult sheep positive on PCR revealed characteristic lesions of OPA on gross and histopathological examination.
  
  
  CONCLUSIONS
  In the absence of known specific antibody response to the infection with JSRV, there is no diagnostic serological test available. The PCR assay employed in this study on lung tissues, using primers based on the U3 region of the viral long terminal repeat for JSRV would be helpful in the screening of preclinical and clinical cases of OPA in sheep.
authors.affiliation: Animal Health Division, ICAR-Central Sheep and Wool Research Institute, Avikanagar, Malpura, Tonk, Rajasthan, India.
authors.forename: Ganesh G
authors.initials: GG
authors.lastname: Sonawane
authors.affiliation: ICAR-National Research Centre on Equines, Hisar, Haryana, India.
authors.forename: Bhupendra Nath
authors.initials: BN
authors.lastname: Tripathi
authors.affiliation: Animal Biotechnology Section, ICAR-Central Sheep and Wool Research Institute, Avikanagar, Malpura, Tonk, Rajasthan, India.
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Animal Health Division, ICAR-Central Sheep and Wool Research Institute, Avikanagar, Malpura, Tonk, Rajasthan, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Kumar
keywords: diagnosis; lung tissues; ovine pulmonary adenomatosis; sheep