title: Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway.
abstract: Catalpol, an iridoid glucoside, has been reported to inhibit apoptosis of neuron and endothelial cells. In the present study, we investigated the mechanism of catalpol-mediated cardioprotection. The rat embryonic ventricular myocardial cell line (H9c2) cells were first incubated with catalpol, and then exposed to hydrogen peroxide (H2O2). The concentration of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were all determined by using commercially available kits. Apoptotic cells were assessed by Hoechst 33258 and Annexin V-fluorescein isothiocyanate binding assay. Synthesis of Bcl-2, Bax, cytochrome c and caspase-3 were analysed by real-time semiquantitative reverse transcription-PCR and Western blotting. We observed that apoptosis in H9c2 was associated with increased Bax, cytochrome c, caspase-3, decreased Bcl-2 activity after 24 h of H2O2 exposure. Catalpol pretreatment afforded a marked protection against the above H2O2-mediated cytotoxicity and apoptosis in H9c2 cells. Moreover, the catalpol pretreatment led to a great reduction in H2O2-induced MDA release and increased SOD. These findings indicated for the first time that pretreatment of H9c2 cells with catalpol can be against H2O2-induced apoptosis, and the protective effect of catalpol involves the mitochondrial-dependent caspase pathway and is associated with increased Bcl-2 and decreased Bax expression.
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Jining Medical College, Jining, Shandong 272100, China hulingai2004@126.com.
authors.forename: Ling-Ai
authors.initials: LA
authors.lastname: Hu
authors.affiliation: Department of Prosthodontics, Jining Stomatology Hospital, Jining, Shandong 272100, China.
authors.forename: Yu-Kun
authors.initials: YK
authors.lastname: Sun
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Jining Medical College, Jining, Shandong 272100, China.
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Jining Medical College, Jining, Shandong 272100, China.
authors.forename: Jin-Guo
authors.initials: JG
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics; D045304:Cytochromes c / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity; D057888:Iridoid Glucosides / Q000008:administration & dosage*; D008928:Mitochondria / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000235:genetics*
chemical_list: C494828:Bax protein, rat; D057888:Iridoid Glucosides; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C078040:catalpol; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D053148:Caspase 3
keywords: Catalpol; H9c2; apoptosis; hydrogen peroxide