title: Tubeimoside-1 suppresses breast cancer metastasis through downregulation of CXCR4 chemokine receptor expression.
abstract: To examine the effect of TBMS1on breast cancer metastasis, and investigate the potential mechanism by which Tubeimoside- 1 (TBMS1) inhibits the CXCR4 expression in breast cancer cells. The expression of CXCR4 in breast cancer cell lines was determined by immunoblotting and real-time PCR. The effect of TBMS1 on NF-κB binding activity was evaluated by EMSA assay and ChIP analysis. Cell proliferation and invasion were analyzed by MTT assay and transwell invasion assay, respectively. The effect of TBMS1 on breast cancer metastasis was further evaluated in a metastasis model of nude mice. TBMS1 suppressed the expression of CXCR4 through inhibition of NF-κB binding activity. TBMS1 inhibited CXCL12-induced invasion in breast cancer cells, while ectopic expression of CXCR4 abolished the inhibitive activity of TBMS1. TBMS1 suppressed breast cancer metastasis in the metastatic model of nude mice. TBMS1 suppressed the CXCR4-mediated metastasis of breast cancer by inhibiting NF-κB binding activity. [BMB Reports 2016; 49(9): 502-507].
authors.affiliation: Department of Human Anatomy, Medical College of Hunan Normal University, Changsha, Hunan 410006, China.
authors.forename: Yaojin
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Gynaecology and Obstetrics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Department of Thoracic Surgery, Hainan General Hospital, Haikou, Hannan 570311, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D047369:Chromatin Immunoprecipitation; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D016328:NF-kappa B / Q000378:metabolism; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D012503:Saponins / Q000633:toxicity*; D014183:Transplantation, Heterologous; D014315:Triterpenes / Q000633:toxicity*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D019718:Receptors, CXCR4; D012503:Saponins; D014315:Triterpenes; C051084:tubeimoside I