title: Elevated serum IL-35 and increased expression of IL-35-p35 or -EBI3 in CD4(+)CD25(+) T cells in patients with active tuberculosis.
abstract: Despite the recent appreciation of interleukin 35 (IL-35) function in inflammatory diseases, little is known for IL-35 response in patients with active tuberculosis (ATB). In the current study, we demonstrated that ATB patients exhibited increases in serum IL-35 and in mRNA expression of both subunits of IL-35 (p35 and EBI3) in white blood cells and peripheral blood mononuclear cells. Consistently, anti-TB drug treatment led to reduction in serum IL-35 level and p35 or EBI3 expression. TB infection was associated with expression of p35 or EBI3 protein in CD4(+) but not CD8(+) T cells. Most p35(+)CD4(+) T cells and EBI3(+)CD4(+) T cells expressed Treg-associated marker CD25. Our findings may be important in understanding immune pathogenesis of TB. IL-35 in the blood may potentially serve as a biomarker for immune status and prognosis in TB.
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
authors.affiliation: Dongguan 6th People's Hospital Dongguan 523008, China.
authors.forename: Gan-Bin
authors.initials: GB
authors.lastname: Liu
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsNo. 1 Xincheng Road, Dongguan 523808, China; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical CollegeNo. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Jun-Ai
authors.initials: JA
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Fu
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Xiang
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Gao
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Yuan-Bin
authors.initials: YB
authors.lastname: Lu
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsNo. 1 Xincheng Road, Dongguan 523808, China; Department of Clinical Laboratory, Affiliated Hospital of Guangdong Medical collegeZhanjiang 524001, China.
authors.forename: Xian-Jing
authors.initials: XJ
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine Guangzhou 510120, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsNo. 1 Xincheng Road, Dongguan 523808, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of MedicineChicago 60612, Illinois, United States of America.
authors.forename: Wan-Dang
authors.initials: WD
authors.lastname: Wang
authors.affiliation: Dongguan 6th People's Hospital Dongguan 523008, China.
authors.forename: Lai-Long
authors.initials: LL
authors.lastname: Yi
authors.affiliation: Department of Medicine, University of Maryland School of Medicine Baltimore, Maryland 21201, United States of America.
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Zhong
authors.affiliation: Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine Chicago 60612, Illinois, United States of America.
authors.forename: Zheng W
authors.initials: ZW
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsNo. 1 Xincheng Road, Dongguan 523808, China; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical CollegeNo. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Jun-Fa
authors.initials: JF
authors.lastname: Xu
keywords: CD4+CD25+ T cells; EBI3; Human active pulmonary tuberculosis; interleukin-35; p35