title: Adipose‑derived mesenchymal stem cell‑facilitated TRAIL expression in melanoma treatment in vitro.
abstract: Adipose-derived stem cells (ADSCs) may be useful as an efficient vehicle in cell-based gene therapy of human diseases due to their ability to migrate to disease lesions. This study investigated the ability of ADSC‑harbored human tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) cDNA to facilitate TRAIL expression and induce A375 melanoma cell apoptosis as observed using a Transwell co‑culture system. A cell migration assay was used to observe ADSC migration ability. In addition, TRAIL protein expression was successfully detected by western blot analysis in ADSCs after stable transfection of TRAIL cDNA. The Transwell co‑culture system data showed that TRAIL-ADSCs could induce A375 cell apoptosis in a dose‑dependent manner. At the gene level, the killing activity of TRAIL-ADSCs was associated with activation of caspase‑4 and caspase‑8. Collectively, the data from the current study provides preclinical support of ADSC‑facilitated TRAIL expression in the treatment of melanoma. Further investigation is required to evaluate and confirm the in vivo ability of TRAIL-ADSCs in therapy of melanoma in animal models.
authors.affiliation: Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Hai Xia
authors.initials: HX
authors.lastname: Jing
authors.affiliation: Department of Dermatology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: De Jian
authors.initials: de J
authors.lastname: Duan
authors.affiliation: Department of Dermatology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Qing Mei
authors.initials: QM
authors.lastname: Hu
authors.affiliation: Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Tie Chi
authors.initials: TC
authors.lastname: Lei
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D020169:Caspases / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism* / Q000628:therapy; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand; D020169:Caspases