title: Comparison of the effect of bone marrow cells infusion through the portal vein and inferior vena cava combined with short-term rapamycin on allogeneic islet grafts in diabetic rats.
abstract: OBJECTIVE
  The study aimed to compare the impact of allogeneic bone marrow cells (BMCs) infusion through the inferior vena cava (IVC) and portal vein (PV) combined with rapamycin on allogeneic islet grafts in diabetic rats.
  
  
  METHODS
  Recipient diabetic Wistar rats were infused with islets from Sprague-Dawley rats through the PV. PKH26-labeled BMCs of Sprague-Dawley rats were infused to recipients through the PV or IVC, followed by administration of rapamycin for 4 days. Blood glucose level was measured to evaluate the survival time of the islets. Lymphocytes separated from blood, BMCs, thymus, liver, spleen and lymph node were analyzed by flow cytometry. The peripheral blood smear, BMCs smear and frozen sections of tissues were observed by a fluorescence microscope.
  
  
  RESULTS
  The survival time of the islets was significantly prolonged by the BMCs infusion combined with rapamycin. The rats receiving BMCs infusion through the PV induced a significantly longer survival time of the islets, and increased mixed chimeras of allogeneic BMCs in the thymus, liver, spleen and lymph node compared with the rats receiving BMCs infusion through the IVC. The amount of the mixed chimeras on day 14 was lower than that on day 7 after islet transplantation. Furthermore, PV transplantation had significantly more mixed chimera than IVC transplantation in all analyzed organs or tissues.
  
  
  CONCLUSIONS
  BMCs infusion combined with rapamycin prolongs the islets survival and induces mixed chimeras of BMCs. PV infusion of BMCs might be a more effective strategy than IVC infusion of BMCs.
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Qingzhen
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Ruibin
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Wanjun
authors.initials: W
authors.lastname: Ren
authors.affiliation: Department of Nephrology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001786:Blood Glucose; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D004195:Disease Models, Animal; D006085:Graft Survival; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D020123:Sirolimus / Q000008:administration & dosage*; D014018:Tissue Distribution; D014184:Transplantation, Homologous / Q000379:methods; D014682:Vena Cava, Inferior
chemical_list: D001786:Blood Glucose; D020123:Sirolimus
keywords: Bone marrow cell; Islet grafts; Rapamycin