title: Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells.
abstract: Mass cytometry is a novel platform for high-dimensional phenotypic and functional analysis of single cells. This system uses elemental metal isotopes conjugated to monoclonal antibodies to evaluate up to 42 parameters simultaneously on individual cells with minimal overlap between channels. The platform can be customized for analysis of both phenotypic and functional markers. Here, we will describe methods to stain, collect, and analyze intracellular functional markers and surface phenotypic markers on natural killer cells.
authors.affiliation: Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Kay
authors.affiliation: Stanford Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Dara M
authors.initials: DM
authors.lastname: Strauss-Albee
authors.affiliation: Stanford Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA. cblish@stanford.edu.|Department of Medicine, Stanford University School of Medicine, 300 Pasteur Dr., Lane Building, L134, Stanford, CA, 94305-5107, USA. cblish@stanford.edu.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Blish
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007554:Isotopes; D007694:Killer Cells, Natural / Q000166:cytology* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D010641:Phenotype; D059010:Single-Cell Analysis
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007554:Isotopes
keywords: CyTOF; Flow cytometry; Intracellular cytokine staining; Mass cytometry; Natural killer cell