title: MHC class I BFIV gene polymorphisms in four Chinese native chicken breeds.
abstract: The major histocompatibility complex (MHC) includes the most polymorphic genes in vertebrates, and balancing selection has been proposed as a main evolutionary force. Here we present one of the first data sets examining the genetic characteristics of chicken MHC I BFIV molecules in four Chinese native breeds, sourced from different regions in China. In all, 89 BFIV alleles were isolated from 102 individuals sampled, and 13 repeated alleles were observed. No significant correlation was found between genetic differentiation and geographical distance in the phylogenetic tree. BFIV genes exhibited a high level of nucleotide polymorphisms, and most of the polymorphic sites were located in the peptide-binding region (PBR) encoded in exons 2 and 3. A comparison of the three-dimensional structures of PBRs in chicken BFIV and human HLA-A molecules revealed evident structural and functional similarities. The results suggested that MHC I molecules had similar structural features in different species.
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China. Electronic address: daiyin2020@163.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Xue-Lan
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Tang
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
authors.forename: Xiao-Miao
authors.initials: XM
authors.lastname: Hu
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
authors.forename: Xue-Huai
authors.initials: XH
authors.lastname: Shen
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China. Electronic address: 1693805400@qq.com.
authors.forename: Dan-Jun
authors.initials: DJ
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D017951:Antigen Presentation; D000941:Antigens / Q000378:metabolism; D001947:Breeding; D002645:Chickens / Q000276:immunology*; D002681:China; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011485:Protein Binding; D040681:Structural Homology, Protein
chemical_list: D000941:Antigens; D015395:Histocompatibility Antigens Class I; D010455:Peptides
keywords: Chinese native chicken; MHC class I BFIV gene; Peptide-binding region; Polymorphism