title: Analysis of Plasma microRNA Associated with Hemolysis.
abstract: We analyzed the effect of hemolysis on microRNA profi le of blood plasma. It was found that hemolysis of ~0.05% erythrocytes in a sample signifi cantly affected the concentration of 9 microRNA: hsa-miR-486-5p, hsa-miR-16-5p, hsa-miR-451a, hsa-miR-106a-5p, hsa-miR-17-5p, hsa-miR-93-5p, hsa-miR-20a-5p, hsa-miR-107, and hsa-miR-20b-5p. The effect of hemolysis on plasma content of miR-17 family microRNA was demonstrated.
authors.affiliation: BioClinicum Research and Development Center, Moscow, Russia. mshkurnikov@imscs.msu.ru.|National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia. mshkurnikov@imscs.msu.ru.
authors.forename: M Yu
authors.initials: MY
authors.lastname: Shkurnikov
authors.affiliation: BioClinicum Research and Development Center, Moscow, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Knyazev
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: K A
authors.initials: KA
authors.lastname: Fomicheva
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Mikhailenko
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: K M
authors.initials: KM
authors.lastname: Nyushko
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E K
authors.initials: EK
authors.lastname: Saribekyan
authors.affiliation: BioClinicum Research and Development Center, Moscow, Russia.
authors.forename: T R
authors.initials: TR
authors.lastname: Samatov
authors.affiliation: National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: B Ya
authors.initials: BY
authors.lastname: Alekseev
mesh_terms: D015415:Biomarkers / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D035683:MicroRNAs / Q000097:blood*
chemical_list: D015415:Biomarkers; D006454:Hemoglobins; D035683:MicroRNAs
keywords: blood plasma; hemolysis; microRNA; microarray analysis