title: Effective method of chitosan-coated alginate nanoparticles for target drug delivery applications.
abstract: In the present study, alginate nanoparticles were firstly prepared for paclitaxel (PTX) delivery with an average size of 200 ± 21 nm. To improve the stability and targeting effect, the chitosan (CS) and folate-chitosan (FA-CS) were introduced to form PTX-loaded CS/ALG NPs and FA-CS/ALG NPs by a new double emulsion cross-linking electrostatic attraction method. The optimization chitosan concentration was 0.5% obtained from the experiment results. The CS/ALG-PTX NPs and FA-CS/ALG-PTX NPs had the average particle size of 306.9 ± 12.9 nm and 283.6 ± 19.2 nm with the zeta potential of 31.1 ± 1.3 mV and -2.98 ± 0.7 mV, and had higher drug loading and entrapment efficiencies than ALG-PTX NPs. The in vitro drug release profile along with release kinetics and mechanism from PTX-loaded NPs were studied under two simulated physiological conditions. Further, the in vitro anti-cancer activity of nanoparticles and the cellular uptake of nanoparticles on HepG2 cells were investigated. The results demonstrated that alginate, CS/ALG and FA-CS/ALG can be used as nanoformulation drug carriers by our new method, and FA-CS/ALG was a promising vehicle for anticancer drug targeted delivery system.
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, P. R. China Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing, PR China wangfang@njfu.edu.cn chaobo.huang@njfu.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, P. R. China.
authors.forename: Siqian
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yuan
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, P. R. China Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing, PR China.
authors.forename: Qinwei
authors.initials: Q
authors.lastname: Gao
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing, P. R. China Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing, PR China wangfang@njfu.edu.cn chaobo.huang@njfu.edu.cn.
authors.forename: Chaobo
authors.initials: C
authors.lastname: Huang
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size
chemical_list: D000464:Alginates; D000970:Antineoplastic Agents; D020099:Coated Materials, Biocompatible; D006603:Hexuronic Acids; D053769:Nanocapsules; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Alginate; chitosan; folate-chitosan; nanoparticles; paclitaxel