title: MicroRNA-34a inhibits cell proliferation and induces cell apoptosis of glioma cells via targeting of Bcl-2.
abstract: Glioblastoma is a highly malignant brain tumor, characterized by the poor prognosis and high recurrence rates. Despite therapeutic strategies including surgery, radiotherapy and chemotherapy, the median survival of patients is only 14.6 months. MicroRNAs (miRNAs) have been considered as a novel type of gene regulator. Previous studies have demonstrated that the expression of miRNA‑34a (miR‑34a) is significantly associated with the grade and prognosis of glioma. However, the exact function of miR‑34a on glioma progression and underlying mechanisms remain to be elucidated. The present study investigated the function of miR‑34a in U87 human glioma cells by exogenously transfecting cells with an miR‑34a mimic. Overexpression of miR‑34a inhibited proliferation, and induced apoptosis of U87 cells. The current study also demonstrated that B‑cell lymphoma 2 (Bcl‑2) was the target gene of miR‑34a, as demonstrated by luciferase assays. Furthermore, restoring the expression of Bcl‑2 was indicated to partially block the miR‑34a‑induced apoptosis. Thus, data from the present study identified miR‑34a as a tumor suppressor in glioma by, at least partially, targeting Bcl‑2. This may provide future novel diagnostic and therapeutic strategies for human glioma.
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Duan
authors.affiliation: Department of Pediatric Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Kejun
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Duan
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics*; D001665:Binding Sites; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D034622:RNA Interference
chemical_list: D020413:3' Untranslated Regions; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D020169:Caspases