title: ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects.
abstract: ADAM (a disintegrin and metalloprotease) constitutes a family of multi-domain proteins that are involved in development, homeostasis, and disease. ADAM12 plays important roles in myogenesis and adipogenesis in mice; however, the precise physiological mechanisms are not known, and the function of this gene in other vertebrates has not been examined. In this study, we used a simple model vertebrate, the zebrafish, to investigate the functions of ADAM12 during development. Zebrafish adam12 is conserved with those of mammals in the synteny and the amino-acid sequence. We examined adam12 expression in zebrafish embryos by whole mount in situ hybridization and the promoter activity of the adam12 upstream sequence. We found that adam12 is strongly expressed in the cardiovascular system, erythroid progenitors, brain, and jaw cartilage during zebrafish development, and adam12-knockout zebrafish exhibited reduced body size in the juvenile stage without apparent morphological defects. Taken together, these results suggest that adam12 plays a significant role in the regulation of body growth during juvenile stage in zebrafish, although the precise molecular mechanisms await further study.
authors.affiliation: Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in Kawahara-cho 53, Sakyo-ku, Japan.
authors.forename: Yudai
authors.initials: Y
authors.lastname: Tokumasu
authors.affiliation: Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in Kawahara-cho 53, Sakyo-ku, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Iida
authors.affiliation: Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in Kawahara-cho 53, Sakyo-ku, Japan.|Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies, Japan.
authors.forename: Zi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ansai
authors.affiliation: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kinoshita
authors.affiliation: Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Shogo-in Kawahara-cho 53, Sakyo-ku, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Sehara-Fujisawa
mesh_terms: D000072199:ADAM12 Protein / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009990:Oryzias / Q000196:embryology / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins; D000072199:ADAM12 Protein
keywords: ADAM; body growth; knockout; zebrafish