title: Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing.
abstract: To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The number of alleles per locus ranged from 1 to 9; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.751, respectively. These markers should be useful tools for genetic variation and conservation studies of B. schreberi.
authors.affiliation: College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, China.
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Long
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
authors.forename: Q F
authors.initials: QF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.
authors.forename: K
authors.initials: K
authors.lastname: Liao
mesh_terms: D000483:Alleles; D056727:Endangered Species; D006579:Heterozygote; D019684:Magnoliopsida / Q000235:genetics*; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic