title: Immunoassay Analysis of Kanamycin in Serum Using the Tobramycin Kit.
abstract: Kanamycin is one of the aminoglycosides used in the treatment of multidrug-resistant tuberculosis. Blood concentrations of kanamycin are predictive for the treatment efficacy and the occurrence of side effects, and dose adjustments can be needed to optimize therapy. However, an immunoassay method for the quantification of kanamycin is not commercially available. We modified the existing tobramycin immunoassay to analyze kanamycin. This modified method was tested in a concentration range of 0.3 to 80.0 mg/liter for inaccuracy and imprecision. In addition, the analytical results of the immunoassay method were compared to those obtained by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method using Passing and Bablok regression. Within-day imprecision varied from 2.3 to 13.3%, and between-day imprecision ranged from 0.0 to 11.3%. The inaccuracy ranged from -5.2 to 7.6%. No significant cross-reactivity with other antimicrobials and antiviral agents was observed. The results of the modified immunoassay method were comparable with the LC-MS/MS analytical outcome. This new immunoassay method enables laboratories to perform therapeutic drug monitoring of kanamycin without the need for complex and expensive LC-MS/MS equipment.
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy and Pharmacology, Groningen, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: Dijkstra
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy and Pharmacology, Groningen, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Voerman
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy and Pharmacology, Groningen, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Greijdanus
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy and Pharmacology, Groningen, The Netherlands University of Groningen, Department of Pharmacy, Division of Pharmacokinetics, Toxicology and Targeting, Groningen, The Netherlands.
authors.forename: D J
authors.initials: DJ
authors.lastname: Touw
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Clinical Pharmacy and Pharmacology, Groningen, The Netherlands j.w.c.alffenaar@umcg.nl.
authors.forename: J W C
authors.initials: JW
authors.lastname: Alffenaar
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood / Q000276:immunology; D000998:Antiviral Agents / Q000276:immunology; D002853:Chromatography, Liquid / Q000379:methods; D003429:Cross Reactions / Q000276:immunology; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007612:Kanamycin / Q000097:blood* / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D044967:Serum / Q000276:immunology*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014031:Tobramycin / Q000276:immunology*
chemical_list: D000890:Anti-Infective Agents; D000998:Antiviral Agents; D011933:Reagent Kits, Diagnostic; D007612:Kanamycin; D014031:Tobramycin