title: Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology.
abstract: Commonly used tests based on wild-type viruses, such as immunostaining, cannot meet the demands for rapid detection of viral replication, high-throughput screening for antivirals, as well as for tracking viral proteins or virus transport in real time. Notably, the development of replicating-competent reporter-expressing viruses (RCREVs) has provided an excellent option to detect directly viral replication without the use of secondary labeling, which represents a significant advance in virology. This article reviews the applications of RCREVs in diagnostic and molecular virology, including rapid neutralization tests, high-throughput screening systems, identification of viral receptors and virus-host interactions, dynamics of viral infections in vitro and in vivo, vaccination approaches and others. However, there remain various challenges associated with RCREVs, including pathogenicity alterations due to the insertion of a reporter gene, instability or loss of the reporter gene expression, or attenuation of reporter signals in vivo. Despite all these limitations, RCREVs have become powerful tools for both basic and applied virology with the development of new technologies for generating RCREVs, the inventions of novel reporters and the better understanding of regulation of viral replication.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. 15204662904@126.com.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. lilianfeng124603@163.com.
authors.forename: Lian-Feng
authors.initials: LF
authors.lastname: Li
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. yushaoxiong1987@hotmail.com.
authors.forename: Shaoxiong
authors.initials: S
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. wangxiao2037@126.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. tdzlk1991@163.com.
authors.forename: Lingkai
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. yujiahui2014@126.com.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. xielibao2015@163.com.
authors.forename: Libao
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Chemistry, College of Arts and Sciences, Georgia State University, Atlanta, GA 30302, USA. wli22@gsu.edu.
authors.forename: Weike
authors.initials: W
authors.lastname: Li
authors.affiliation: University of Karachi, Karachi 75270, Pakistan. razimalikhan@gmail.com.
authors.forename: Razim
authors.initials: R
authors.lastname: Ali
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China. huajiqiu@hvri.ac.cn.
authors.forename: Hua-Ji
authors.initials: HJ
authors.lastname: Qiu
mesh_terms: D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D014773:Virology / Q000379:methods*; D014779:Virus Replication; D014780:Viruses / Q000235:genetics* / Q000254:growth & development*
keywords: high-throughput screening; molecular virology; replicating-competent virus; reporter