title: Reinforcement of freeze-dried chitosan scaffolds with multiphasic calcium phosphate short fibers.
abstract: The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM). The mechanical properties of the scaffolds were assessed by compression test. The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds. The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively. The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid. The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
authors.affiliation: Bioceramics and Implants Laboratory, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439955941, Islamic Republic of Iran. Electronic address: mohamadiz@ut.ac.ir.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Mohammadi
authors.affiliation: Bioceramics and Implants Laboratory, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439955941, Islamic Republic of Iran. Electronic address: asmesgar@ut.ac.ir.
authors.forename: Abdorreza Sheikh-Mehdi
authors.initials: AS
authors.lastname: Mesgar
authors.affiliation: Bioceramics and Implants Laboratory, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439955941, Islamic Republic of Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Rasouli-Disfani
mesh_terms: D001672:Biocompatible Materials; D002130:Calcium Phosphates / Q000737:chemistry*; D002131:Calcium Pyrophosphate / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D048271:Chitosan; D002131:Calcium Pyrophosphate
keywords: Bone scaffold; Calcium phosphate short fibers; Chitosan; Composite; Mechanical reinforcment