title: Is the ISAC 112 Microarray Useful in the Diagnosis of Pollinosis in Spain?
abstract: BACKGROUND
  Multiple sensitization is frequent among pollen-allergic patients. The goal of this study was to determine the diagnostic accuracy of the ImmunoCAP ISAC 112 (ISAC112) microarray in allergy to pollen from several taxa and its clinical utility in a Spanish population.
  
  
  METHODS
  Specific IgE was determined in 390 pollen-allergic patients using the ISAC112 microarray. Diagnostic accuracy (sensitivity, specificity, predictive values, and area under the ROC curve) was calculated for the diagnosis of allergy to pollen from grass (n=49), cypress (n=75), olive tree (n=33), plane tree (n=63), and pellitory of the wall (n=17) and compared with that of the singleplex ImmunoCAP immunoassay.
  
  
  RESULTS
  The sensitivity of the ISAC112 microarray ranged from 68.2% for allergy to plane tree pollen to 93.9% for allergy to grass pollen. The specificity was >90%. The AUC for the diagnosis of allergy to plane tree pollen was 0.798, whereas the AUC for the remaining cases was ≥0.876. The accuracy of ISAC112 was higher than that of ImmunoCAP for plane tree pollen and similar for the remaining pollens. The frequency of sensitization to most species-specific allergenic components and profilins varied between the different geographical regions studied. A total of 73% of pollen-allergic patients were sensitized to species-specific components of more than 1 pollen type.
  
  
  CONCLUSIONS
  The ISAC112 microarray is an accurate tool for the diagnosis of allergy to pollen from grass, cypress, olive tree, plane tree, and pellitory of the wall. The features of the ISAC112 microarray are similar or superior (in the case of plane tree pollen) to those of ImmunoCAP. This microarray is particularly useful for the etiologic diagnosis of pollinosis in patients sensitized to multiple pollen species whose pollination periods overlap.
authors.affiliation: Allergology Service, Complejo Hospitalario de Navarra, Pamplona, Spain.|IdiSNA, Navarra Institute for Health Research, Spain.
authors.forename: B E
authors.initials: BE
authors.lastname: García
authors.affiliation: IdiSNA, Navarra Institute for Health Research, Spain.|Allergology and Clinical Immunology Department, Clínica Universidad de Navarra, Pamplona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Martínez-Aranguren
authors.affiliation: Allergology and Clinical Immunology Department, Clínica Universidad de Navarra, Pamplona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Bernard Alonso
authors.affiliation: Allergology Service, Hospital de Basurto, Bilbao, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Gamboa
authors.affiliation: Allergology Service, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Feo Brito
authors.affiliation: Unitat d'Alèrgia Servei Pneumologia i Alèrgia Respiratoria Universitat de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (CIBERES), Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bartra
authors.affiliation: Allergology Service, Hospital Infanta Leonor, Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Blanca-López
authors.affiliation: Allergology Service, IBIMA, Hospital Regional Universitario de Malaga, UMA, Malaga, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Gómez
authors.affiliation: Allergology Service, Hospital Virgen del Puerto, Plasencia, Cáceres, Spain.
authors.forename: M I
authors.initials: MI
authors.lastname: Alvarado
authors.affiliation: Allergology Service, Hospital General Universitario de Alicante, Alicante, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Fernández
authors.affiliation: Allergology Service, Hospital de Alcorcón, Alcorcón, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Alonso
authors.affiliation: Allergology Service, Hospital de Fuenlabrada, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gonzalez-Mancebo
authors.affiliation: Allergology Service, Complejo Hospitalario Torrecárdenas, Almería, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Moya
authors.affiliation: Allergology Service, Hospital de A Coruña, A Coruña, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Parra
authors.affiliation: Allergology Service, Hospital Universitario Ramon y Cajal, IRYCIS, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Terrados
authors.affiliation: Allergology Service, Complejo Hospitalario de Navarra, Pamplona, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Sola
authors.affiliation: IdiSNA, Navarra Institute for Health Research, Spain.|Allergology and Clinical Immunology Department, Clínica Universidad de Navarra, Pamplona, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Goikoetxea
authors.affiliation: IdiSNA, Navarra Institute for Health Research, Spain.|Allergology and Clinical Immunology Department, Clínica Universidad de Navarra, Pamplona, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
mesh_terms: D000328:Adult; D000485:Allergens / Q000145:classification / Q000276:immunology*; D019540:Area Under Curve; D005260:Female; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D046228:Microarray Analysis / Q000706:statistics & numerical data*; D008875:Middle Aged; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000145:classification / Q000276:immunology*; D011237:Predictive Value of Tests; D051304:Profilins / Q000097:blood / Q000235:genetics; D012372:ROC Curve; D012130:Respiratory Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000276:immunology* / Q000473:pathology; D013030:Spain; D013045:Species Specificity; D014197:Trees / Q000276:immunology
chemical_list: D000485:Allergens; D051304:Profilins; D007073:Immunoglobulin E
keywords: Accuracy; Microarray; Molecular diagnosis; Pollen allergy