title: Effects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by Klebsiella pneumoniae J2B.
abstract: The current study investigates the impact of mutation of 2,3-butanediol (BDO) formation pathway on glycerol metabolism and 1,3-propanediol (PDO) production by lactate dehydrogenase deficient mutant of Klebsiella pneumoniae J2B. To this end, BDO pathway genes, budA, budB, budC and budO (whole-bud operon), were deleted from K. pneumoniae J2B ΔldhA and the mutants were studied for glycerol metabolism and alcohols (PDO, BDO) production. ΔbudO-mutant-only could completely abolish BDO production, but with reductions in cell growth and PDO production. By modifying the culture medium, the ΔbudO mutant could recover its performance on the flask scale. However, in bioreactor experiments, the ΔbudO mutant accumulated a significant amount of pyruvate (>73mM) in the late phase and PDO production stopped concomitantly. Glycolytic intermediates of glycerol, especially glyceraldehyde-3-phosphate (G3P) was highly inhibitory to glycerol dehydratase (GDHt); its accumulation, followed by pyruvate accumulation, was assumed to be responsible for the ΔbudO mutant's low PDO production.
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea; Nottingham BBSRC/EPSRC Synthetic Biology Research Centre, Centre for Biomolecular Sciences, University Park, The University of Nottingham, Nottingham NG7 2RD, United Kingdom.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea.
authors.forename: Meetu
authors.initials: M
authors.lastname: Durgapal
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea.
authors.forename: Mugesh
authors.initials: M
authors.lastname: Sankaranarayanan
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea.
authors.forename: Ashok
authors.initials: A
authors.lastname: Somasundar
authors.affiliation: R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Chelladurai
authors.initials: C
authors.lastname: Rathnasingh
authors.affiliation: R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea.
authors.forename: HyoHak
authors.initials: H
authors.lastname: Song
authors.affiliation: R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Doyoung
authors.initials: D
authors.lastname: Seung
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea. Electronic address: parksh@pusan.ac.kr.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
mesh_terms: D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000502:physiology*; D002072:Butylene Glycols / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D009154:Mutation / Q000502:physiology*; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D011409:Propylene Glycols; C026978:2,3-butylene glycol; C041787:1,3-propanediol; D005990:Glycerol
keywords: 1,3-Propanediol; 2,3-Butanediol; Glycerol; Klebsiella pneumoniae J2B; ΔbudO mutant