title: Detection of beta-tubulin in the cytoplasm of the interphasic Entamoeba histolytica trophozoites.
abstract: It is known that the microtubules (MT) of Entamoeba histolytica trophozoites form an intranuclear mitotic spindle. However, electron microscopy studies and the employment of anti-beta-tubulin (β-tubulin) antibodies have not exhibited these cytoskeletal structures in the cytoplasm of these parasites. The purpose of this work was to detect β-tubulin in the cytoplasm of interphasic E. histolytica trophozoites. Activated or non-activated HMI-IMSS-strain E. histolytica trophozoites were used and cultured for 72 h at 37 °C in TYI-S-33 medium, and then these were incubated with the anti-β-tubulin antibody of E. histolytica. The anti-β-tubulin antibody reacted with the intranuclear mitotic spindle of E. histolytica-activated trophozoites as control. In contrast, in non-activated interphasic parasites, anti-β-tubulin antibody reacted with diverse puntiform structures in the cytoplasm and with ring-shaped structures localized in the cytoplasm, cellular membrane and endocytic stomas. In this work, for the first time, the presence of β-tubulin is shown in the cytoplasm of E. histolytica trophozoites.
authors.affiliation: División de Investigación en Salud, Unidad Médica de Alta Especialidad (UMAE), Hospital de Especialidades, Centro Médico Nacional General de División "Manuel Ávila Camacho", Instituto Mexicano del Seguro Social (IMSS), Calle 2 Norte 2004, Col. Centro, 72000 Puebla, Mexico; Laboratorio de Investigación en Inmunobiología, Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), Calle 13 Sur 2706, Col. Volcanes, 72410 Puebla, Mexico. Electronic address: gom_cond@yahoo.com.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gómez-Conde
authors.affiliation: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Av. Instituto Politécnico Nacional 2508, Delegación Gustavo A. Madero, Col.San Pedro Zacatenco, 07360 México, D.F., Mexico. Electronic address: mavargas@cinvestav.mx.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Vargas-Mejía
authors.affiliation: Laboratorio de Investigación en Inmunobiología, Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), Calle 13 Sur 2706, Col. Volcanes, 72410 Puebla, Mexico. Electronic address: diazorea@yahoo.com.mx.
authors.forename: María Alicia
authors.initials: MA
authors.lastname: Díaz-Orea
authors.affiliation: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Av. Instituto Politécnico Nacional 2508, Delegación Gustavo A. Madero, Col.San Pedro Zacatenco, 07360 México, D.F., Mexico. Electronic address: rohernan@cinvestav.mx.
authors.forename: Rosaura
authors.initials: R
authors.lastname: Hernández-Rivas
authors.affiliation: Laboratorio de Investigación en Inmunobiología, Facultad de Medicina, Benemérita Universidad Autónoma de Puebla (BUAP), Calle 13 Sur 2706, Col. Volcanes, 72410 Puebla, Mexico. Electronic address: marielcardenasperea@yahoo.com.mx.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Cárdenas-Perea
authors.affiliation: Servicio de Traumatología y Ortopedia, Hospital Regional 1(o) de Octubre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado (ISSSTE), Av. Instituto Politécnico Nacional 1669, Gustavo A. Madero, Col. Magdalena de las Salinas, Del. Gustavo A. Madero, 07760 México D.F., Mexico. Electronic address: tayger.19@hotmail.com.
authors.forename: Tayde
authors.initials: T
authors.lastname: Guerrero-González
authors.affiliation: Laboratorio de Medicina Genómica, Hospital Regional 1 de Octubre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado (ISSSTE), Avenida Instituto Politécnico Nacional N° 1669, Gustavo A. Madero, Col. Magdalena de las Salinas, Del. Gustavo A. Madero, 07760 México, D.F., Mexico. Electronic address: jantgonzalez@issste.gob.mx.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: González-Barrios
authors.affiliation: Jefatura de División de Investigación en Salud, UMAE, Hospital de Traumatología, Instituto Mexicano del Seguro Social, Diagonal Defensores de la República y 6 Poniente, Col. Amor, 72140 Puebla, Mexico. Electronic address: alvaro.montielj@imss.gob.mx.
authors.forename: Álvaro José
authors.initials: ÁJ
authors.lastname: Montiel-Jarquín
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D004748:Entamoeba histolytica / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D015151:Immunoblotting; D007399:Interphase; D051379:Mice; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry; D008941:Spindle Apparatus / Q000648:ultrastructure; D053058:Trophozoites / Q000737:chemistry; D014404:Tubulin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D014404:Tubulin
keywords: Cytoplasm; Endocytic estomas; Entamoeba histolytica; β-Tubulin