title: Analysis of lipoprotein profiles of healthy cats by gel-permeation high-performance liquid chromatography.
abstract: Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as a method to obtain a feline lipoprotein profile. The lipoprotein profiles showed large and small peaks, which corresponded to high-density lipoprotein (HDL) and low-density lipoprotein (LDL), respectively, whereas very low-density lipoprotein (VLDL) and chylomicron (CM) were only marginally detected. This profile was very similar to that of dogs reported previously. Healthy cats also had a small amount of cholesterol-rich particles distinct from the normal LDL or HDL profile. There was no difference in lipoprotein profiles between the sexes, but males had a significantly larger LDL particle size (P=0.015). This study shows the feasibility of GP-HPLC for obtaining accurate lipoprotein profiles with small sample volumes and provides valuable reference data for healthy cats that should facilitate diagnoses.
authors.affiliation: Veterinary Internal Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan, Musashino, Tokyo 180-8602, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Mizutani
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
authors.forename: Hiroko
authors.initials: H
authors.lastname: Okuda
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Arai
authors.forename: Koji
authors.initials: K
authors.lastname: Kuriyama
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mori
authors.forename: Itaru
authors.initials: I
authors.lastname: Yoshimura
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Koyama
mesh_terms: D000818:Animals; D002415:Cats / Q000097:blood* / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D002914:Chylomicrons / Q000097:blood; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male
chemical_list: D002914:Chylomicrons; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D002784:Cholesterol