title: Transformation of tobacco plants by Yali PPO-GFP fusion gene and observation of subcellular localization.
abstract: To explore the subcellular localization of Polyphenol oxidase (PPO) from Pyrus bretschneideri, the 1779 bp cDNA of PPO gene excluding the termination codon TAA was cloned and fused with GFP to construct a binary vector pBI121-PPO-GFP. Then, the binary vector was transformed into Nicotiana tabacum by the tumefanciens-mediated method. Using confocal laser scanning microscopy, green fluorescent signals were localized in chloroplasts of the transformed Nicotiana tabacum cell, suggesting that the Polyphenol oxidase from Pyrus bretschneideri was a chloroplast protein.
authors.affiliation: College of Biology Science and Engineering, Hebei University of Economics and Business Shijiazhuang 050061, Hebei, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: College of Biology Science and Engineering, Hebei University of Economics and Business Shijiazhuang 050061, Hebei, China.
authors.forename: Gui-Qin
authors.initials: GQ
authors.lastname: Li
authors.affiliation: Hebei Women's Vocational College Shijiazhuang 050091, Hebei, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Dong
authors.affiliation: College of Biology Science and Engineering, Hebei University of Economics and Business Shijiazhuang 050061, Hebei, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
keywords: GFP; PPO; Yali; gene fusion; subcellular localization; transformation