title: Age Estimation in Living Egyptians Using Signal Joint T-cell Receptor Excision Circle Rearrangement.
abstract: Age estimation is one of the challenges in forensic sciences. There are many techniques to estimate the age. Molecular biology approach is one of these techniques. Signal joint T-cell receptor excision circles gene (sjTRECs), is one of this approach. We aimed to use sjTRECs as a suitable marker for age estimation among Egyptian population. TaqMan qPCR approach was used to quantify sjTREC levels in blood samples obtained from 153 healthy Egyptian individuals ranging from a few weeks to 70 years. Our results showed a significant negative correlation between sjTREC levels and age with p ≤ 0.05. Moreover, the individual's age can be determined through this formula Age = -30.671+ (-5.998Y) (Y is dCtTBP - sjTREC) with standard error ±7.35 years. Within the forensic context, sjTREC' levels can be used to estimate the Egyptian individual's age accurately.
authors.affiliation: Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Kasr Al Ainy St., Cairo, 11562, Egypt.
authors.forename: Samah F
authors.initials: SF
authors.lastname: Ibrahim
authors.affiliation: Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Kasr Al Ainy St., Cairo, 11562, Egypt.
authors.forename: Iman F
authors.initials: IF
authors.lastname: Gaballah
authors.affiliation: Medical Biochemistry, Faculty of Medicine, Cairo University, Kasr Al Ainy St., Cairo, 11562, Egypt.
authors.forename: Laila A
authors.initials: LA
authors.lastname: Rashed
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging; D004247:DNA; D004534:Egypt; D053803:Forensic Genetics; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D013950:Thymus Gland
chemical_list: D004247:DNA
keywords: DNA typing; Egyptian; age estimation; forensic science; polymerase chain reaction; signal joint T-cell receptor excision circles