title: Noise exposure modulates cochlear inner hair cell ribbon volumes, correlating with changes in auditory measures in the FVB/nJ mouse.
abstract: Cochlear neuropathy resulting from unsafe noise exposure is a life altering condition that affects many people. This hearing dysfunction follows a conserved mechanism where inner hair cell synapses are lost, termed cochlear synaptopathy. Here we investigate cochlear synaptopathy in the FVB/nJ mouse strain as a prelude for the investigation of candidate genetic mutations for noise damage susceptibility. We used measurements of auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE) to assess hearing recovery in FVB/nJ mice exposed to two different noise levels. We also utilized confocal fluorescence microscopy in mapped whole mount cochlear tissue, in conjunction with deconvolution and three-dimensional modeling, to analyze numbers, volumes and positions of paired synaptic components. We find evidence for significant synapse reorganization in response to both synaptopathic and sub-synaptopathic noise exposures in FVB/nJ. Specifically, we find that the modulation in volume of very small synaptic ribbons correlates with the presence of reduced ABR peak one amplitudes in both levels of noise exposures. These experiments define the use of FVB/nJ mice for further genetic investigations into the mechanisms of noise damage. They further suggest that in the cochlea, neuronal-inner hair cell connections may dynamically reshape as part of the noise response.
authors.affiliation: Department of Neuroscience, University of Rochester School of Medicine and Dentistry, Box 603, 601 Elmwood Avenue, Rochester, NY, 14642, USA.
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Paquette
authors.affiliation: Department of Neuroscience, University of Rochester School of Medicine and Dentistry, Box 603, 601 Elmwood Avenue, Rochester, NY, 14642, USA.
authors.forename: Felicia
authors.initials: F
authors.lastname: Gilels
authors.affiliation: Department of Neuroscience, University of Rochester School of Medicine and Dentistry, Box 603, 601 Elmwood Avenue, Rochester, NY, 14642, USA.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: White
mesh_terms: D000818:Animals; D006199:Hair Cells, Auditory, Inner / Q000502:physiology*; D006320:Hearing Tests; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009622:Noise; D013569:Synapses / Q000473:pathology*; D000160:Vestibulocochlear Nerve Diseases / Q000473:pathology*