title: Bacillus licheniformis Contains Two More PerR-Like Proteins in Addition to PerR, Fur, and Zur Orthologues.
abstract: The ferric uptake regulator (Fur) family proteins include sensors of Fe (Fur), Zn (Zur), and peroxide (PerR). Among Fur family proteins, Fur and Zur are ubiquitous in most prokaryotic organisms, whereas PerR exists mainly in Gram positive bacteria as a functional homologue of OxyR. Gram positive bacteria such as Bacillus subtilis, Listeria monocytogenes and Staphylococcus aureus encode three Fur family proteins: Fur, Zur, and PerR. In this study, we identified five Fur family proteins from B. licheniformis: two novel PerR-like proteins (BL00690 and BL00950) in addition to Fur (BL05249), Zur (BL03703), and PerR (BL00075) homologues. Our data indicate that all of the five B. licheniformis Fur homologues contain a structural Zn2+ site composed of four cysteine residues like many other Fur family proteins. Furthermore, we provide evidence that the PerR-like proteins (BL00690 and BL00950) as well as PerRBL (BL00075), but not FurBL (BL05249) and ZurBL (BL03703), can sense H2O2 by histidine oxidation with different sensitivity. We also show that PerR2 (BL00690) has a PerR-like repressor activity for PerR-regulated genes in vivo. Taken together, our results suggest that B. licheniformis contains three PerR subfamily proteins which can sense H2O2 by histidine oxidation not by cysteine oxidation, in addition to Fur and Zur.
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Ji
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Shin-Yeong
authors.initials: SY
authors.lastname: Ju
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Yoon-Mo
authors.initials: YM
authors.lastname: Yang
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Ryu
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kwon
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Young-Bin
authors.initials: YB
authors.lastname: Won
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Yeh-Eun
authors.initials: YE
authors.lastname: Lee
authors.affiliation: Department of Biology, California State University Fresno, Fresno, California, 93740-8034, United States of America.
authors.forename: Hwan
authors.initials: H
authors.lastname: Youn
authors.affiliation: Department of Life Science and Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000069976:Bacillus licheniformis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010084:Oxidation-Reduction; D012097:Repressor Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D006861:Hydrogen Peroxide; D015032:Zinc