title: 3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening.
abstract: 2D cell culture and preclinical animal models have traditionally been implemented for investigating the underlying cellular mechanisms of human disease progression. However, the increasing significance of 3D vs. 2D cell culture has initiated a new era in cell culture research in which 3D in vitro models are emerging as a bridge between traditional 2D cell culture and in vivo animal models. Additive manufacturing (AM, also known as 3D printing), defined as the layer-by-layer fabrication of parts directed by digital information from a 3D computer-aided design file, offers the advantages of simultaneous rapid prototyping and biofunctionalization as well as the precise placement of cells and extracellular matrix with high resolution. In this review, we highlight recent advances in 3D printing of tissue engineered constructs that recapitulate the physical and cellular properties of the tissue microenvironment for investigating mechanisms of disease progression and for screening drugs.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, 2301 Vanderbilt Place, Nashville, TN, 37232, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Vanderburgh
authors.affiliation: Department of Veterans Affairs, Tennessee Valley Healthcare System, 1235 MRB IV, 2222 Pierce Ave, Nashville, TN, 37232, USA.|Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Cancer Biology, Vanderbilt University, Nashville, TN, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Sterling
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, 2301 Vanderbilt Place, Nashville, TN, 37232, USA. scott.guelcher@vanderbilt.edu.|Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN, USA. scott.guelcher@vanderbilt.edu.|Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA. scott.guelcher@vanderbilt.edu.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Guelcher
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008954:Models, Biological; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*
keywords: 3D printing; Additive Manufacturing; Bioprinting; Drug screening; Tissue engineered constructs