title: Proteomic Assessment of the Relevant Factors Affecting Pork Meat Quality Associated withLongissimus dorsiMuscles in Duroc Pigs.
abstract: Meat quality is a complex trait influenced by many factors, including genetics, nutrition, feeding environment, animal handling, and their interactions. To elucidate relevant factors affecting pork quality associated with oxidative stress and muscle development, we analyzed protein expression in high qualitylongissimus dorsimuscles (HQLD) and low qualitylongissimus dorsimuscles (LQLD) from Duroc pigs by liquid chromatographytandem mass spectrometry (LC-MS/MS)-based proteomic analysis. Between HQLD (n = 20) and LQLD (n = 20) Duroc pigs, 24 differentially expressed proteins were identified by LC-MS/MS. A total of 10 and 14 proteins were highly expressed in HQLD and LQLD, respectively. The 24 proteins have putative functions in the following seven categories: catalytic activity (31%), ATPase activity (19%), oxidoreductase activity (13%), cytoskeletal protein binding (13%), actin binding (12%), calcium ion binding (6%), and structural constituent of muscle (6%). Silver-stained image analysis revealed significant differential expression of lactate dehydrogenase A (LDHA) between HQLD and LQLD Duroc pigs. LDHA was subjected toin vitrostudy of myogenesis under oxidative stress conditions and LDH activity assay to verification its role in oxidative stress. No significant difference of mRNA expression level of LDHA was found between normal and oxidative stress condition. However, LDH activity was significantly higher under oxidative stress condition than at normal condition usingin vitromodel of myogenesis. The highly expressed LDHA was positively correlated with LQLD. Moreover, LDHA activity increased by oxidative stress was reduced by antioxidant resveratrol. This paper emphasizes the importance of differential expression patterns of proteins and their interaction for the development of meat quality traits. Our proteome data provides valuable information on important factors which might aid in the regulation of muscle development and the improvement of meat quality inlongissimus dorsimuscles of Duroc pigs under oxidative stress conditions.
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Jin Hyoung
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Ra Ham
authors.initials: RH
authors.lastname: Lee
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.|National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Jeon
authors.affiliation: Pohang Center for Evaluation of Biomaterials, Pohang 37668, Korea.
authors.forename: Seon-Min
authors.initials: SM
authors.lastname: Park
authors.affiliation: Pohang Center for Evaluation of Biomaterials, Pohang 37668, Korea.
authors.forename: Jae-Cheon
authors.initials: JC
authors.lastname: Shin
authors.affiliation: Aging Research Institute, Korea Research Institute of Bioscience & BioTechnology, Daejeon 34141, Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea.
authors.forename: Hyun-Sung
authors.initials: HS
authors.lastname: Kang
authors.affiliation: Department of Animal Science, College of Agricultural and Life Science, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Nag-Jin
authors.initials: NJ
authors.lastname: Choi
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea.
authors.forename: Kang Seok
authors.initials: KS
authors.lastname: Seo
authors.affiliation: Department of Pharmacy, Keimyung University, Daegu 704-701, Korea.
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Cho
authors.affiliation: Department of Biomedical Engineering, Konyang University, Daejeon 35365, Korea.
authors.forename: MinSeok S
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Applied Bioscience, CHA University, Seongnam 463-836, Korea.
authors.forename: Sungho
authors.initials: S
authors.lastname: Ko
authors.affiliation: Department of Prosthodontics, School of Dentistry and Institute of Oral Bio-Science and Research Institute of Clinical Medicine, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Seo
authors.affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Seung-Youp
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Mokpo 534-729, Korea.|The China-US (Henan) Hormel Cancer Institute, Zhengzhou, Henan 127, China.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Shim
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Chae
keywords: Duroc; Liquid Chromatography-tandem Mass Spectrometry; Longissimus dorsi Muscle; Meat Quality; Oxidative Stress