title: The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts.
abstract: We investigated the effects of different implant biomaterials on cultured canine bone marrow stromal cells (BMSC) undergoing differentiation into osteoblasts (dBMSC). BMSC were isolated from canine humerus by marrow aspiration, cultured and differentiated on calcium phosphate scaffold (CPS), hydroxyapatite, hydroxyapatite in gel form and titanium mesh. We used the MTT method to determine the effects of osteogenic media on proliferation. The characteristics of dBMSC were assessed using alizarin red (AR), immunocytochemistry and osteoblastic markers including alkaline phosphatase/von Kossa (ALP/VK), osteocalcin (OC) and osteonectin (ON), and ELISA. The morphology of dBMSC on the biomaterials was investigated using inverted phase contrast microscopy and scanning electron microscopy. We detected expression of ALP/VK, AR, OC and ON by day 7 of culture; expression increased from day 14 until day 21. CPS supported the best adhesion, cell spreading, proliferation and differentiation of BMSCs. The effects of the biomaterials depended on their surface properties. Expression of osteoblastic markers showed that canine dBMSCs became functional osteoblasts. Tissue engineered stem cells can be useful clinically for autologous implants for treating bone wounds.
authors.affiliation: a Department of Biology , Faculty of Life Science, Celal Bayar University , Manisa , Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Özdal-Kurt
authors.affiliation: b Department of Histology and Embryology , Faculty of Medicine, Celal Bayar University , Manisa , Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Tuğlu
authors.affiliation: b Department of Histology and Embryology , Faculty of Medicine, Celal Bayar University , Manisa , Turkey.|f Experimental Health Science Research Center, Near East University , Lefkosa , North Cyprus.
authors.forename: H S
authors.initials: HS
authors.lastname: Vatansever
authors.affiliation: c Veterinary Department of Konak Municipality , Izmir , Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Tong
authors.affiliation: d Endodontology, School of Dentistry, Ege University , Izmir , Turkey.
authors.forename: B H
authors.initials: BH
authors.lastname: Şen
authors.affiliation: e Endodontology, School of Dentistry, Katip Celebi University , Izmir , Turkey.
authors.forename: S I
authors.initials: SI
authors.lastname: Deliloğlu-Gürhan
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D004195:Disease Models, Animal; D004285:Dogs; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D013194:Staining and Labeling
chemical_list: D000880:Anthraquinones; D001672:Biocompatible Materials; D015415:Biomarkers; C010078:alizarin
keywords: biomaterials; bone marrow; canine; differentiation; implants; osteoblast; stromal cells; tissue engineering