title: Platelets are not hyperreactive in patients with ovarian cancer.
abstract: Paraneoplastic thrombocytosis has been reported in different types of solid tumors, including ovarian epithelial cancer, and found to be associated with a worse outcome. Although the effect of cancer on increasing platelet counts is well documented, the effect of cancer on platelet functions is not well known. We compared in vitro aggregation response of platelets isolated from 34 patients with ovarian cancer to those of platelets from 19 patients with benign ovarian tumors. Aggregation studies were conducted in a light transmission aggregometer, using both a high and a low dose of ADP and collagen. We evaluated platelet preactivation by measuring the plasma concentration of β-thromboglobulin (β-TG) and platelet factor-4 (PF-4) as markers of platelet α granule secretion, using ELISA. We found that ovarian cancer is not associated with an enhanced aggregation response of platelets to ADP or collagen, and plasma concentration of β-TG and PF-4 is not higher in patients with ovarian cancer compared to those in patients with benign ovarian tumors.
authors.affiliation: a Section of Benign Hematology , The University of Texas MD Anderson Cancer Center , Houston , Texas , USA.
authors.forename: Shuju
authors.initials: S
authors.lastname: Feng
authors.affiliation: a Section of Benign Hematology , The University of Texas MD Anderson Cancer Center , Houston , Texas , USA.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Kroll
authors.affiliation: b Department of Gynecologic Oncology and Reproductive Medicine , The University of Texas MD Anderson Cancer Center , Houston , Texas , USA.
authors.forename: Alpa M
authors.initials: AM
authors.lastname: Nick
authors.affiliation: b Department of Gynecologic Oncology and Reproductive Medicine , The University of Texas MD Anderson Cancer Center , Houston , Texas , USA.|c Center for RNA Interference and Non-Coding RNA , The University of Texas MD Anderson Cancer Center , Houston , Texas USA.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sood
authors.affiliation: a Section of Benign Hematology , The University of Texas MD Anderson Cancer Center , Houston , Texas , USA.
authors.forename: Vahid
authors.initials: V
authors.lastname: Afshar-Kharghan
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000097:blood* / Q000150:complications; D015539:Platelet Activation; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D013922:Thrombocytosis / Q000209:etiology
chemical_list: D000244:Adenosine Diphosphate; D003094:Collagen
keywords: Ovarian cancer; platelet; platelet aggregation; platelet factor-4; β-thromboglobulin