title: Electroporation of siRNA to Silence Gene Expression in Primary NK Cells.
abstract: Gene silencing through siRNA is an effective experimental tool to unravel molecular mechanisms involved in cellular processes. Here we describe a method to silence gene expression in primary human natural killer (NK) cells by transfecting ON-TARGETplus SMART pool siRNA using an electroporation-based method called Nucleofection(®). The technique yields effective silencing of the target gene without any off-target effects.
authors.affiliation: Division of Pediatrics, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, 77030, USA.
authors.forename: Prasad V
authors.initials: PV
authors.lastname: Phatarpekar
authors.affiliation: Division of Pediatrics, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, 77030, USA.
authors.forename: Dean A
authors.initials: DA
authors.lastname: Lee
authors.affiliation: Division of Pediatrics, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, 77030, USA. sssomanchi@mdanderson.org.
authors.forename: Srinivas S
authors.initials: SS
authors.lastname: Somanchi
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D018274:Electroporation / Q000379:methods*; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering
keywords: Electroporation; Gene silencing; Human primary NK cell; Nucleofection; ON-TARGETplus; SMART pool siRNA; siRNA