title: Increase in pro-apoptotic Bax expression and decrease in anti-apoptotic Bcl-2 expression in newborns with necrotizing enterocolitis.
abstract: The aim of the present study was to find out if there is an increase in the expression of pro-apoptotic Bax and reduction in expression of anti-apoptotic Blc-2A1 in newborn intestines with necrotizing enterocolitis (NEC).
  
  
  
  We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry.
  
  
  
  We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry.
  
  
  
  Our data in humannewborns suggest that alteration of the balance between pro-apoptotic Bax expression and anti-apoptotic Bcl-2A1 expression in the site of injury is a possible mechanism in the pathogenesis of NEC.
authors.affiliation: Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey. drufukates@gmail.com.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Ates
authors.affiliation: Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey.
authors.forename: Gulnur
authors.initials: G
authors.lastname: Gollu
authors.affiliation: Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey.
authors.forename: Gonul
authors.initials: G
authors.lastname: Kucuk
authors.affiliation: Ankara University School of Medicine, Department of Histology and Embryology, Ankara, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Billur
authors.affiliation: Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey.
authors.forename: Meltem
authors.initials: M
authors.lastname: Bingol-Kologlu
authors.affiliation: Zekai Tahir Burak Woman Health Education and Research Hospital, Department of Pediatric Surgery, Ankara, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Yılmaz
authors.affiliation: Zekai Tahir Burak Woman Health Education and Research Hospital, Department of Pediatric Surgery, Ankara, Turkey.
authors.forename: Hulya
authors.initials: H
authors.lastname: Ozkan-Ulu
authors.affiliation: Ankara University School of Medicine, Department of Histology and Embryology, Ankara, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Bayram
authors.affiliation: Gazi University, School of Medicine, Immunology Research Center, Ankara, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Bagriacik
authors.affiliation: Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Dindar
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D020345:Enterocolitis, Necrotizing / Q000378:metabolism*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D015778:Minor Histocompatibility Antigens / Q000096:biosynthesis* / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000502:physiology; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000502:physiology
chemical_list: C096816:BCL2-related protein A1; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein
keywords: Apoptosis; Gene expression; Necrotizing enterocolitis; Newborn