title: Inhibition of mammalian target of rapamycin activation in the rostral anterior cingulate cortex attenuates pain-related aversion in rats.
abstract: Pain is a complex experience that comprises both sensory and affective dimensions. Mammalian target of rapamycin (mTOR) plays an important role in the modulation of neuronal plasticity associated with the pathogenesis of pain sensation. However, the role of mTOR in pain affect is unclear. Using a formalin-induced conditioned place avoidance (F-CPA) test, the current study investigated the effects of the mTOR specific inhibitor rapamycin on noxious stimulation induced aversion in the rostral anterior cingulate cortex (rACC). Intraplantar injection of 5% formalin was associated with significant activation of mTOR, as well as p70 ribosomal S6 protein (p70S6K), its downstream effector, in the rACC. The inhibition of mTOR activation with rapamycin disrupted pain-related aversion; however, this inhibition did not affect formalin-induced spontaneous nociceptive behaviors in rats. These findings demonstrated for the first time that mTOR and its downstream pathway in the rACC contribute to the induction of pain-related negative emotion.
authors.affiliation: Department of Anesthesia, Ningbo NO. 2 Hospital, Ningbo 315010, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Anesthesia, Yinzhou Hospital Affiliated to Medical School of Ningbo University, Ningbo 315040, PR China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Anesthesia, Hangzhou Hospital Affiliated to Nanjing Medical University, Hangzhou First People's Hospital, Hangzhou 310006, PR China.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Anesthesia, Hangzhou Hospital Affiliated to Nanjing Medical University, Hangzhou First People's Hospital, Hangzhou 310006, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Xiao
authors.affiliation: Department of Anesthesia, Ningbo NO. 2 Hospital, Ningbo 315010, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Meng
authors.affiliation: Department of Anesthesia, Ningbo NO. 2 Hospital, Ningbo 315010, PR China.
authors.forename: Jinwei
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Anesthesia, Ningbo NO. 2 Hospital, Ningbo 315010, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Anesthesia, Ningbo NO. 2 Hospital, Ningbo 315010, PR China.
authors.forename: Ruichun
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Anesthesia, Ningbo NO. 2 Hospital, Ningbo 315010, PR China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Anesthesia, Ningbo NO. 2 Hospital, Ningbo 315010, PR China. Electronic address: 13858222873@163.com.
authors.forename: Junping
authors.initials: J
authors.lastname: Chen
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D004644:Emotions / Q000187:drug effects / Q000502:physiology; D005557:Formaldehyde; D006179:Gyrus Cinguli / Q000187:drug effects / Q000378:metabolism*; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D059226:Nociceptive Pain / Q000188:drug therapy / Q000378:metabolism* / Q000523:psychology; D058748:Pain Perception / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology; D013037:Spatial Behavior / Q000187:drug effects / Q000502:physiology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000700:Analgesics; D005557:Formaldehyde; C546845:mTOR protein, rat; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus
keywords: Anterior cingulate cortex; Conditioned place avoidance; Conditioned place preference; Mammalian target of rapamycin; Pain-related aversion; Rapamycin