title: In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart.
abstract: During embryogenesis, the epicardial contribution to coronary vasculature development has been very well established. Cells derived from the epicardium differentiate into smooth muscle cells, fibroblasts and endothelial cells that contribute to the formation of coronary vessels. Here we have established an in vitro culture method for embryonic epicardial cells. Using genetic labelling, we have demonstrated that the majority of the migrating cells in our explant culture are of epicardial origin. Epicardial explant cells also retain the expression of epicardial markers (Wt1 and Tbx18). Furthermore, we provide evidence that epicardial explant cells undergo epithelial to mesenchymal transition (EMT), migrate and differentiate into smooth muscle cells after Transforming growth factor beta 1 (TGF-β1) treatment in a manner indistinguishable from that of epicardial cells in vivo. In conclusion, we provide a novel method for the culture of embryonic epicardial cells, which will help to explore the role of specific genes in epicardial cell biology.
authors.affiliation: Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School.
authors.forename: Sindhu
authors.initials: S
authors.lastname: Ramesh
authors.affiliation: Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School.
authors.forename: Anamika
authors.initials: A
authors.lastname: Singh
authors.affiliation: Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School.
authors.forename: Dasan M
authors.initials: DM
authors.lastname: Cibi
authors.affiliation: Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School; National Heart Research Institute Singapore, National Heart Centre Singapore; The Hatter Cardiovascular Insititute, University College London.
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Hausenloy
authors.affiliation: Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School; National Heart Research Institute Singapore, National Heart Centre Singapore; manvendra.singh@duke-nus.edu.sg.
authors.forename: Manvendra K
authors.initials: MK
authors.lastname: Singh
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D003331:Coronary Vessels / Q000166:cytology / Q000196:embryology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D038081:Organogenesis; D010496:Pericardium / Q000166:cytology* / Q000196:embryology; D011247:Pregnancy