title: Cytotoxic Aporphines from Artabotrys crassifolius.
abstract: Artabotrys crassifolius Hook. f. & Thomson is a medicinal plant used in Malaysia. The cytotoxic effects of the hexane, chloroform and ethanol extracts of the leaves and bark were examined in vitro against MCF-7, MDA-468 and HCT-116 cells. The chloroform extract of the bark inhibited the growth of all cell lines with GI₅₀ values ranging from 4.2 µg/mL to 9.4 µg/mL. Silica gel column chromatography of this extract yielded artabotrine, liridine, atherospermidine and lysicamine. Artabotrine and lysicamine inhibited the growth of HCT-116 and MCF-7 cells with GI₅₀ values ranging from 3.3 µM to 3.9 µM. These alkaloids were not toxic to human embryonic kidney cells (HEK297) up to a concentration of 50 µg/mL.
authors.forename: Tan Kok
authors.initials: TK
authors.lastname: Kwan
authors.forename: Fiona
authors.initials: F
authors.lastname: Shipton
authors.forename: Nadiah Syafiqah Nor
authors.initials: NS
authors.lastname: Azman
authors.forename: Shahadat
authors.initials: S
authors.lastname: Hossan
authors.forename: Khoo Ten
authors.initials: KT
authors.lastname: Jin
authors.forename: Christophe
authors.initials: C
authors.lastname: Wiart
mesh_terms: D029061:Annonaceae / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic