title: Proper autophagy is indispensable for angiogenesis during chick embryo development.
abstract: People have known that autophagy plays a very important role in many physiological and pathological events. But the role of autophagy on embryonic angiogenesis still remains obscure. In this study, we demonstrated that Atg7, Atg8 and Beclin1 were expressed in the plexus vessels of angiogenesis at chick yolk sac membrane and chorioallantoic membrane. Interfering in autophagy with autophagy inducer or inhibitor could restrict the angiogenesis in vivo, which might be driven by the disorder of angiogenesis-related gene expressions, and also lead to embryonic hemorrhage, which was due to imperfection cell junctions in endothelial cells including abnormal expressions of tight junction, adheren junction and desmosome genes. Using HUVECs, we revealed that cell viability and migration ability changed with the alteration of cell autophagy exposed to RAPA or 3-MA. Interestingly, tube formation assay showed that HUVECs ability of tube formation altered with the change of Atg5, Atg7 and Atg8 manipulated by the transfection of their corresponding siRNA or plasmids. Moreover, the lost cell polarity labeled by F-actin and the absenced β-catenin in RAPA-treated and 3-MA-treated cell membrane implied intracellular cytoskeleton alteration was induced by the activation and depression of autophagy. Taken together, our current experimental data reveal that autophagy is really involved in regulating angiogenesis during embryo development.
authors.affiliation: a Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College , Jinan University , Guangzhou , China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Lu
authors.affiliation: a Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College , Jinan University , Guangzhou , China.
authors.forename: Yu-Xun
authors.initials: YX
authors.lastname: Shi
authors.affiliation: a Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College , Jinan University , Guangzhou , China.
authors.forename: Zheng-Lai
authors.initials: ZL
authors.lastname: Ma
authors.affiliation: a Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College , Jinan University , Guangzhou , China.
authors.forename: Guang
authors.initials: G
authors.lastname: Wang
authors.affiliation: b Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering , Jinan University , Guangzhou , China.
authors.forename: Langxia
authors.initials: L
authors.lastname: Liu
authors.affiliation: c Division of Cell and Developmental Biology , University of Dundee , Dundee , UK.
authors.forename: Manli
authors.initials: M
authors.lastname: Chuai
authors.affiliation: d Key Laboratory of Medical Cell Biology , China Medical University , Shenyang , China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Song
authors.affiliation: e School of Biological Sciences , University of East Anglia , Norwich , UK.
authors.forename: Andrea
authors.initials: A
authors.lastname: Münsterberg
authors.affiliation: d Key Laboratory of Medical Cell Biology , China Medical University , Shenyang , China.
authors.forename: Liu
authors.initials: L
authors.lastname: Cao
authors.affiliation: a Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College , Jinan University , Guangzhou , China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D016888:Angiodysplasia / Q000235:genetics / Q000473:pathology; D000818:Animals; D001343:Autophagy / Q000187:drug effects / Q000235:genetics; D000071183:Autophagy-Related Proteins / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D004730:Endothelium, Vascular / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055785:Gene Knockdown Techniques; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000235:genetics; D020123:Sirolimus / Q000494:pharmacology
chemical_list: D000071183:Autophagy-Related Proteins; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; C025946:3-methyladenine; D000225:Adenine; D020123:Sirolimus
keywords: Atg; angiogenesis; autophagy; chorioallantoic membrane; yolk sac membrane