title: Methylation of DAPK and THBS1 genes in esophageal gastric-type columnar metaplasia.
abstract: OBJECTIVE
  To explore methylation of DAPK, THBS1, CDH-1, and p14 genes, and Helicobacter pylori (H. pylori) status in individuals harboring esophageal columnar metaplasia.
  
  
  METHODS
  Distal esophageal mucosal samples obtained by endoscopy and histologically diagnosed as gastric-type (non-specialized) columnar metaplasia, were studied thoroughly. DNA was extracted from paraffin blocks, and methylation status of death-associated protein kinase (DAPK), thrombospondin-1 (THBS1), cadherin-1 (CDH1), and p14 genes, was examined using a methyl-sensitive polymerase chain reaction (MS-PCR) and sodium bisulfite modification protocol. H. pylori cagA status was determined by PCR.
  
  
  RESULTS
  In total, 68 subjects (33 females and 35 males), with a mean age of 52 years, were included. H. pylori cagA positive was present in the esophageal gastric-type metaplastic mucosa of 18 individuals. DAPK, THSB1, CDH1, and p14 gene promoters were methylated by MS-PCR in 40 (58.8%), 33 (48.5%), 46 (67.6%), and 23 (33.8%) cases of the 68 esophageal samples. H. pylori status was associated with methylation of DAPK (P = 0.003) and THBS1 (P = 0.019).
  
  
  CONCLUSIONS
  DNA methylation occurs in cases of gastric-type (non-specialized) columnar metaplasia of the esophagus, and this modification is associated with H. pylori cagA positive infection.
authors.affiliation: Roberto Herrera-Goepfert, José Luis Mosqueda-Vargas, Department of Pathology, Instituto Nacional de Cancerología (INCan), 10480 México, DF, México.
authors.forename: Roberto
authors.initials: R
authors.lastname: Herrera-Goepfert
authors.affiliation: Roberto Herrera-Goepfert, José Luis Mosqueda-Vargas, Department of Pathology, Instituto Nacional de Cancerología (INCan), 10480 México, DF, México.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Oñate-Ocaña
authors.affiliation: Roberto Herrera-Goepfert, José Luis Mosqueda-Vargas, Department of Pathology, Instituto Nacional de Cancerología (INCan), 10480 México, DF, México.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Mosqueda-Vargas
authors.affiliation: Roberto Herrera-Goepfert, José Luis Mosqueda-Vargas, Department of Pathology, Instituto Nacional de Cancerología (INCan), 10480 México, DF, México.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Herrera
authors.affiliation: Roberto Herrera-Goepfert, José Luis Mosqueda-Vargas, Department of Pathology, Instituto Nacional de Cancerología (INCan), 10480 México, DF, México.
authors.forename: Clementina
authors.initials: C
authors.lastname: Castro
authors.affiliation: Roberto Herrera-Goepfert, José Luis Mosqueda-Vargas, Department of Pathology, Instituto Nacional de Cancerología (INCan), 10480 México, DF, México.
authors.forename: Julia
authors.initials: J
authors.lastname: Mendoza
authors.affiliation: Roberto Herrera-Goepfert, José Luis Mosqueda-Vargas, Department of Pathology, Instituto Nacional de Cancerología (INCan), 10480 México, DF, México.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: González-Barrios
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000235:genetics; D015703:Antigens, CD; D001426:Bacterial Proteins / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D015820:Cadherins / Q000235:genetics; D019175:DNA Methylation; D064550:Death-Associated Protein Kinases / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D005260:Female; D016480:Helicobacter pylori / Q000235:genetics; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies; D019700:Thrombospondin 1 / Q000235:genetics*; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D015703:Antigens, CD; D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D019700:Thrombospondin 1; D025803:Tumor Suppressor Protein p14ARF; C080866:cagA protein, Helicobacter pylori; C000611726:thrombospondin-1, human; D064550:Death-Associated Protein Kinases
keywords: DNA methylation; Death-associated protein kinase; Esophageal columnar metaplasia; Helicobacter pylori; Thrombospondin-1; cagA