title: Rhizome extracts of Curcuma zedoaria Rosc induce caspase dependant apoptosis via generation of reactive oxygen species in filarial parasite Setaria digitata in vitro.
abstract: Human lymphatic filariasis (LF) is mainly caused by filarial parasite Wuchereria bancrofti and is the second leading cause of long term and permanent disability in tropical countries. To date, incapability to eliminate long lived adult parasites by current drugs remains the major challenge in the elimination of LF. Hence, in the current study, the efficacy of rhizome extracts of Curcuma zedoaria (a plant traditionally used in Sri Lanka in the management of LF) was evaluated as an effective filaricide in vitro. Sequential solvent extracts of C. zedoaria rhizomes were screened for in vitro antifilarial activity at 0.01-1 mg/mL concentrations by motility inhibition assay and 3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide (MTT) reduction assay using cattle parasite Setaria digitata as a model organism. Exposure of parasites to hexane and chloroform extracts of C. zedoaria caused a dose dependant reduction in motility and viability of microfilariae (IC50 = 72.42 μg/mL for hexane extract, 191.14 μg/mL for chloroform extract) and adult parasites (IC50 = 77.07 μg/mL for hexane extract, 259.87 μg/mL for chloroform extract). Both extracts were less toxic to human peripheral blood mononuclear cells when compared to filariae. A dose dependant increase in caspase 3/CED 3 and a decrease in total protein content, cyclooxygenase (COX) and protein tyrosine phosphatase (PTP) activities were observed in adult parasites treated with hexane or chloroform extract. A significant degree of chromatin condensation and apoptotic body formation were also observed in these worms by Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) staining respectively. Dose dependant chromosomal DNA laddering was observed in treated adult worms but not in microfilariae in response to both extracts. Oxidative stress parameters such as reduction in reduced glutathione (GSH) levels and increase in glutathione s transferase (GST), superoxide dismutase (SOD) and catalase activities, increased reactive oxygen levels (ROS) and lipid peroxidation were also observed indicating that an apoptotic event is induced by reactive oxygen species.
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 03, Sri Lanka. Electronic address: senathilake@gmail.com.
authors.forename: K S
authors.initials: KS
authors.lastname: Senathilake
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 03, Sri Lanka. Electronic address: erick@ibmbb.cmb.ac.lk.
authors.forename: E H
authors.initials: EH
authors.lastname: Karunanayake
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 03, Sri Lanka. Electronic address: sam@ibmbb.cmb.ac.lk.
authors.forename: S R
authors.initials: SR
authors.lastname: Samarakoon
authors.affiliation: Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 03, Sri Lanka. Electronic address: kamani@ibmbb.cmb.ac.lk.
authors.forename: K H
authors.initials: KH
authors.lastname: Tennekoon
authors.affiliation: Department of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka. Electronic address: edilip.desilva@gmail.com.
authors.forename: E D
authors.initials: ED
authors.lastname: de Silva
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002417:Cattle; D030024:Curcuma / Q000737:chemistry*; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D020128:Inhibitory Concentration 50; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D010529:Peritoneal Cavity / Q000469:parasitology; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D000069292:Pyroptosis / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D027343:Rhizome / Q000737:chemistry*; D017182:Setaria Nematode / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D053148:Caspase 3
keywords: Anthelmintic; Curcuma; Elephantiasis; Filariasis; Microfilaria; Parasite; Setaria nematode