title: Role of MTHFR C677T and MTR A2756G polymorphisms in thyroid and breast cancer development.
abstract: Folate metabolism is essential for DNA synthesis and repair. Alterations in genes that participate in folate metabolism can be associated with several types of malignant neoplasms, including thyroid and breast cancer. In the present case-control study, we examined the association between methylenetetrahydrofolate reductase (MTHFR C677T, rs1801133) and methionine synthase (MTR A2756G, rs1805087) polymorphisms and risk for thyroid and breast cancer. Polymerase chain reaction-restriction fragment length technique was used to determine the specific genotypes in the genes of interest. Statistical analysis was performed by multiple logistic regression test. We found an association between MTHFR C677T polymorphism and risks to both thyroid (OR = 2.50; 95%CI = 1.15-5.46; P = 0.02) and breast cancer (OR = 2.53; 95%CI = 1.08-5.93; P = 0.03). Tobacco consumption and high body mass index were also associated with thyroid cancer. In addition, increased age (≥50 years) and alcohol consumption were found to be associated with breast cancer. Our results indicated that MTHFR C677T is significantly associated with thyroid and breast cancer risks. Thus, these factors may be used as potential prognostic markers for thyroid and breast cancers.
authors.affiliation: Unidade de Pesquisa em Genética e Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: T
authors.initials: T
authors.lastname: Zara-Lopes
authors.affiliation: Unidade de Pesquisa em Genética e Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: A P A
authors.initials: AP
authors.lastname: Gimenez-Martins
authors.affiliation: Unidade de Pesquisa em Genética e Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: C H V
authors.initials: CH
authors.lastname: Nascimento-Filho
authors.affiliation: Unidade de Pesquisa em Genética e Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: M M U
authors.initials: MM
authors.lastname: Castanhole-Nunes
authors.affiliation: Unidade de Pesquisa em Genética e Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: A L S
authors.initials: AL
authors.lastname: Galbiatti-Dias
authors.affiliation: Departamento de Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Hospital de Base de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: J A
authors.initials: JA
authors.lastname: Padovani-Júnior
authors.affiliation: Departamento de Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Hospital de Base de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: J V
authors.initials: JV
authors.lastname: Maniglia
authors.affiliation: Departamento de Ginecologia e Obstetrícia, Hospital de Base de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
authors.forename: J L E
authors.initials: JL
authors.lastname: Francisco
authors.affiliation: Unidade de Pesquisa em Genética e Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.|Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto.
authors.forename: E C
authors.initials: EC
authors.lastname: Pavarino
authors.affiliation: Unidade de Pesquisa em Genética e Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.|Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto.
authors.forename: E M
authors.initials: EM
authors.lastname: Goloni-Bertollo
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D001938:Brazil; D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics; D005492:Folic Acid / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D005492:Folic Acid; D005287:Ferredoxin-NADP Reductase; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; C585801:MTR protein, human