title: Quantum dot-NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo.
abstract: A small luciferase protein (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair. The conjugate showed 76% BRET efficiency and lymph node mapping was successfully performed. The cRGD peptide was conjugated to QD-Nluc for tumor targeting. The self-illuminating QD-Nluc showed excellent energy transfer in a living system and offered an optimal tumor-to-background ratio (>85).
authors.affiliation: Departments of Radiology and Medical Physics, University of Wisconsin-Madison, WI 53705, USA. wcai@uwhealth.org.
authors.forename: Anyanee
authors.initials: A
authors.lastname: Kamkaew
authors.affiliation: Departments of Radiology and Medical Physics, University of Wisconsin-Madison, WI 53705, USA. wcai@uwhealth.org.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Departments of Radiology and Medical Physics, University of Wisconsin-Madison, WI 53705, USA. wcai@uwhealth.org.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: England
authors.affiliation: Departments of Radiology and Medical Physics, University of Wisconsin-Madison, WI 53705, USA. wcai@uwhealth.org and Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Departments of Radiology and Medical Physics, University of Wisconsin-Madison, WI 53705, USA. wcai@uwhealth.org and University of Wisconsin Carbone Cancer Center, Madison, WI 53705, USA.
authors.forename: Weibo
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D060807:Bioluminescence Resonance Energy Transfer Techniques / Q000379:methods; D001932:Brain Neoplasms / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry*; D033364:Decapoda; D005456:Fluorescent Dyes / Q000737:chemistry; D005909:Glioblastoma / Q000000981:diagnostic imaging*; D006801:Humans; D008156:Luciferases / Q000737:chemistry*; D049449:Luminescence; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D051379:Mice; D018613:Microscopy, Confocal; D010456:Peptides, Cyclic / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D010456:Peptides, Cyclic; C507169:cyclic arginine-glycine-aspartic acid peptide; D008156:Luciferases