title: Comparison of antioxidant properties of different therapeutic albumin preparations.
abstract: Albumin displays several important functions for homeostasis amongst which the maintenance of the plasma redox-state. The study aim was to compare the redox state of pharmaceutical human albumin preparations since it reflects the oxidation-reduction status of the surrounding environment. Using an array of analytical methods, four commercially available albumins were compared with respect to their structural characteristics (cobalt ion binding, glycation, spectrophotometric and fluorometric profiles) and their ability to scavenge hydroxyl, peroxyl or free radicals. The different albumins exhibited a similar structural profile as well as hydroxyl and peroxyl scavenging activities. By contrast, the albumin from LFB (Vialebex(®)) possessed a significantly higher capacity to transfer electrons to DPPH, as compared with other albumins that was correlated with the level of free cysteine-34. Commercially available albumins differed for some of their antioxidant properties. The albumin preparation possessing the highest level of free cysteine-34 exhibited the highest antioxidant potential.
authors.affiliation: LFB Biotechnologies, Loos, France. Electronic address: plantierj@lfb.fr.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Plantier
authors.affiliation: LFB Biotechnologies, Loos, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Duretz
authors.affiliation: LFB Biotechnologies, Loos, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Devos
authors.affiliation: LFB Biotechnologies, Loos, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Urbain
authors.affiliation: LFB Biotechnologies, Loos, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Jorieux
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D005609:Free Radicals / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D013053:Spectrophotometry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D012709:Serum Albumin; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D003545:Cysteine
keywords: DPPH; Free cysteine; Human albumin; Redox potential; Therapeutic preparation