title: Modified serpinA1 as risk marker for Parkinson's disease dementia: Analysis of baseline data.
abstract: Early detection of dementia in Parkinson disease is a prerequisite for preventive therapeutic approaches. Modified serpinA1 in cerebrospinal fluid (CSF) was suggested as an early biomarker for differentiation between Parkinson patients with (PDD) or without dementia (PD). Within this study we aimed to further explore the diagnostic value of serpinA1. We applied a newly developed nanoscale method for the detection of serpinA1 based on automated capillary isoelectric focusing (CIEF). A clinical sample of 102 subjects including neurologically healthy controls (CON), PD and PDD patients was investigated. Seven serpinA1 isoforms of different charge were detected in CSF from all three diagnostic groups. The mean CSF signals of the most acidic serpinA1 isoform differed significantly (p < 0.01) between PDD (n = 29) and PD (n = 37) or CON (n = 36). Patients above the cut-off of 6.4 have a more than six times higher risk for an association with dementia compared to patients below the cut off. We propose this serpinA1 CIEF-immunoassay as a novel tool in predicting cognitive impairment in PD patients and therefore for patient stratification in therapeutic trials.
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Halbgebauer
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Nagl
authors.affiliation: LVR-Klinikum Essen, Department of Psychiatry and Psychotherapy, Faculty of Medicine, University of Duisburg-Essen, Germany.|Department of Psychiatry and Psychotherapy, University Medical Center (UMG), Georg-August-University, Göttingen, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Klafki
authors.affiliation: LVR-Klinikum Essen, Department of Psychiatry and Psychotherapy, Faculty of Medicine, University of Duisburg-Essen, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Haußmann
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Steinacker
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Oeckl
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Kassubek
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Elmar
authors.initials: E
authors.lastname: Pinkhardt
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Albert C
authors.initials: AC
authors.lastname: Ludolph
authors.affiliation: Department of Neurology, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland.
authors.forename: Hilkka
authors.initials: H
authors.lastname: Soininen
authors.affiliation: Department of Neurology, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland.
authors.forename: Sanna-Kaisa
authors.initials: SK
authors.lastname: Herukka
authors.affiliation: Department of Psychiatry and Psychotherapy, University Medical Center (UMG), Georg-August-University, Göttingen, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Wiltfang
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Otto
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000134:cerebrospinal fluid* / Q000737:chemistry; D003704:Dementia / Q000175:diagnosis* / Q000473:pathology*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000150:complications*; D011379:Prognosis; D018570:Risk Assessment; D000515:alpha 1-Antitrypsin / Q000134:cerebrospinal fluid* / Q000737:chemistry
chemical_list: D015415:Biomarkers; C043392:SERPINA1 protein, human; D000515:alpha 1-Antitrypsin