title: The effects of bevacizumab on intestinal anastomotic healing in rabbits.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of the preoperative administration of BV on the healing process of intestinal anastomosis in a rabbit model.
  
  
  METHODS
  Twenty male white rabbits were randomly divided into two groups. The control group received saline 1 week before surgery, and the BV group received intravenous BV 1 week before surgery. Each rabbit underwent an enteroenterostomy and a colocolostomy. On postoperative day 7, the bursting pressures of the anastomoses, CD31 and α-smooth muscle actin (α-SMA) staining by immunohistochemistry, the gene expression of α-SMA, and collagen deposition using Picrosirius Red at the site of anastomosis were evaluated.
  
  
  RESULTS
  The bursting pressure of small bowel anastomoses was significantly lower in the BV group than in the control group (control 184 ± 10 mmHg vs. BV 140 ± 9 mmHg; p = 0.004). The microvessel counts in the anastomotic tissue were significantly lower in the BV group than in the control group in both the small bowel (p = 0.023) and colon (p = 0.008). The expression of α-SMA, and the degree of collagen deposition decreased in the anastomotic tissue in the BV group compared with the control group.
  
  
  CONCLUSIONS
  The preoperative use of BV may therefore negatively affect the rigidity of intestinal anastomosis.
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan. yyoko@med.nagoya-u.ac.jp.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Yokoyama
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Uehara
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kokuryo
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Junpei
authors.initials: J
authors.lastname: Yamaguchi
authors.affiliation: Department of Pathology, Japanese Red Cross Nagoya Daini Hospital, Nagoya, Japan.
authors.forename: Toyonori
authors.initials: T
authors.lastname: Tsuzuki
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Nagino
mesh_terms: D000199:Actins / Q000378:metabolism; D000714:Anastomosis, Surgical; D020533:Angiogenesis Inhibitors / Q000008:administration & dosage* / Q000009:adverse effects*; D000818:Animals; D000068258:Bevacizumab / Q000008:administration & dosage* / Q000009:adverse effects*; D003094:Collagen / Q000378:metabolism; D003125:Colostomy; D004766:Enterostomy; D007275:Injections, Intravenous; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects; D057234:Preoperative Period; D011817:Rabbits; D000072836:Surgical Wound / Q000378:metabolism / Q000503:physiopathology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000199:Actins; D020533:Angiogenesis Inhibitors; D000068258:Bevacizumab; D003094:Collagen
keywords: Anastomotic leakage; Angiogenesis; Bevacizumab; Intestinal anastomotic healing; α-Smooth muscle actin