title: Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens.
abstract: The production of chemicals alongside fuels will be essential to enhance the feasibility of lignocellulosic biorefineries. Succinic acid (SA), a naturally occurring C4-diacid, is a primary intermediate of the tricarboxylic acid cycle and a promising building block chemical that has received significant industrial attention. Basfia succiniciproducens is a relatively unexplored SA-producing bacterium with advantageous features such as broad substrate utilization, genetic tractability, and facultative anaerobic metabolism. Here B. succiniciproducens is evaluated in high xylose-content hydrolysates from corn stover and different synthetic media in batch fermentation. SA titers in hydrolysate at an initial sugar concentration of 60g/L reached up to 30g/L, with metabolic yields of 0.69g/g, and an overall productivity of 0.43g/L/h. These results demonstrate that B. succiniciproducens may be an attractive platform organism for bio-SA production from biomass hydrolysates.
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.
authors.forename: Davinia
authors.initials: D
authors.lastname: Salvachúa
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.
authors.forename: Holly
authors.initials: H
authors.lastname: Smith
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.
authors.forename: Peter C
authors.initials: PC
authors.lastname: St John
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Mohagheghi
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Peterson
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.
authors.forename: Brenna A
authors.initials: BA
authors.lastname: Black
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Dowe
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA. Electronic address: gregg.beckham@nrel.gov.
authors.forename: Gregg T
authors.initials: GT
authors.lastname: Beckham
mesh_terms: D000188:Actinobacillus / Q000187:drug effects / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D018533:Biomass; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D019802:Succinic Acid / Q000378:metabolism*; D013464:Sulfuric Acids / Q000494:pharmacology; D003313:Zea mays / Q000737:chemistry
chemical_list: D013464:Sulfuric Acids; C036909:lignocellulose; D008031:Lignin; D019802:Succinic Acid; D005947:Glucose; C033158:sulfuric acid
keywords: Actinobacillus succinogenes; Biochemical; Biorefinery; Corn stover; Xylose