title: Analysis of acrylamide in food products by microchip electrophoresis with on-line multiple-preconcentration techniques.
abstract: In this paper, a microchip electrophoresis method based on on-line multiple-preconcentration techniques combining field-amplified sample stacking and reversed-field stacking was developed for highly efficient analysis of acrylamide in food products. The related mechanism as well as important parameters governing separation and preconcentration have been investigated in order to obtain maximum resolution and sensitivity. The best separation was achieved using a 100mM borate solution at pH 9.3 as running buffer, and a sensitivity enhancement factor of 432 was obtained using this concentration method under optimal conditions. The detection limit of acrylamide was 1ng/mL, which was comparable to those previously obtained using CE methods with on-line preconcentration techniques and was 41-700 times lower than those previously reported CE methods without concentration process. The proposed method also gave satisfactory and reliable results in the analysis of acrylamide in potato chips and French fries.
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China.
authors.forename: Minglei
authors.initials: M
authors.lastname: Wu
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China.
authors.forename: Wujuan
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China.
authors.forename: Guan
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China.
authors.forename: Pingang
authors.initials: P
authors.lastname: He
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China. Electronic address: qjwang@chem.ecnu.edu.cn.
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D001331:Automation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection
chemical_list: D020106:Acrylamide
keywords: Acrylamide; Food; Microchip electrophoresis; Multiple-preconcentration