title: Hydrogen peroxide generation by the Weissberger biogenic oxidative system during hyaluronan degradation.
abstract: By applying the enzyme catalase, our study on hyaluronan degradation confirms the generation of hydrogen peroxide using the Weissberger biogenic oxidative system (WBOS), which is composed of ascorbate and cupric ions. Dynamic viscosities of hyaluronan (HA) solutions influenced by WBOS in the absence and presence of catalase were analysed by rotational viscometry. Molar masses of HAs were determined by size-exclusion chromatography with multi-angle laser-light scattering. Our results show that catalase dose-dependently inhibited the degradation of HA macromolecules, which presumably confirms the generation of H2O2 in the reaction system. This has implications in range of biomedical applications such as arthritic joint treatment, tissue engineering, ocular and cosmetic surgery.
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Slovakia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Valachová
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Slovakia.
authors.forename: Dominika
authors.initials: D
authors.lastname: Topoľská
authors.affiliation: Istituto per lo Studio delle Macromolecole, Consiglio Nazionale delle Ricerche, Italy.
authors.forename: Raniero
authors.initials: R
authors.lastname: Mendichi
authors.affiliation: Stokes Laboratories, University of Limerick, Ireland. Electronic address: Maurice.Collins@ul.ie.
authors.forename: Maurice N
authors.initials: MN
authors.lastname: Collins
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Slovakia.
authors.forename: Vlasta
authors.initials: V
authors.lastname: Sasinková
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Slovakia.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Šoltés
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D002374:Catalase / Q000378:metabolism*; D003300:Copper / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000138:chemical synthesis*; D010084:Oxidation-Reduction
chemical_list: D003300:Copper; D006820:Hyaluronic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; D001205:Ascorbic Acid
keywords: Catalase; Glycosaminoglycans; Reactive oxygen species