title: Fabrication of a liquid-gated enzyme field effect device for sensitive glucose detection.
abstract: This study presents fabrication of a liquid-gated enzyme field effect device and its implementation as a glucose biosensor. The device consisted of four electrodes on a glass substrate with a channel functionalized by carboxylated multi-walled carbon nanotubes-polyaniline nanocomposite (MWCNTCOOH/PAn) and glucose oxidase. The resistance of functionalized channel increased with increasing the concentration of glucose when an electric field was applied to the liquid gate. The most effective and stable performance was obtained at the applied electric field of 100 mV. The device resistance, R, exhibited a linear relationship with the logarithm of glucose concentration in the range between 0.005 and 500 mM glucose. The detection limit (S/N = 3) for glucose was about 0.5 μM. Large effective area and good conductivity properties of MWCNTCOOH/PAn nanocomposite were the key features of the fabricated sensitive and stable glucose biosensor.
authors.affiliation: College of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box 45195-1159, Gava Zang, Zanjan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Fathollahzadeh
authors.affiliation: Department of Electrical and Computer Engineering, University of Tehran, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: College of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box 45195-1159, Gava Zang, Zanjan, Iran; Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran. Electronic address: haghighi@iasbs.ac.ir.
authors.forename: B
authors.initials: B
authors.lastname: Haghighi
authors.affiliation: Department of Electrical and Computer Engineering, University of Tehran, Tehran, Iran. Electronic address: Kolahdouz@ut.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Kolahdouz
authors.affiliation: Department of Electrical and Computer Engineering, University of Tehran, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Fathipour
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000378:metabolism*; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis; D005949:Glucose Oxidase / Q000378:metabolism*; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning
chemical_list: D001786:Blood Glucose; D005949:Glucose Oxidase
keywords: Diabetes; Glucose; Glucose biosensor; Liquid-gated enzyme field effect device; MWCNTs-polyaniline nanocomposite