title: Gelatin Directly Enhances Neurogenic Differentiation Potential in Bone Marrow-Derived Mesenchymal Stem Cells Without Stimulation of Neural Progenitor Cell Proliferation.
abstract: Gelatin has been reported to induce generation of mesenchymal stem cells (MSCs) with enhanced potential of differentiation into neuronal lineage cells. However, the presence of various cell types besides MSCs in bone marrow has raised doubts about the effects of gelatin. In the following report, we determined whether gelatin can directly enhance neurogenic differentiation potential in MSCs without proliferation of neural progenitor cells (NPCs). MSCs comprised a high proportion of bone marrow-derived primary cells (BMPCs) and gelatin induced significant increases in MSC proliferation during primary culture, and the proportion of MSCs was maintained at more than 99% throughout the subculture. However, NPCs comprised a low percentage of BMPCs and a decrease in proliferation was detected despite gelatin treatment during the primary culture, and the proportion of subcultured NPCs gradually decreased. In a similar manner, MSCs exposed to gelatin during primary culture showed more enhanced neurogenic differentiation ability than those not exposed to gelatin. Together, these results demonstrate that gelatin directly enhances neurogenic differentiation in bone marrow-derived MSCs without stimulating NPC proliferation.
authors.affiliation: 1 Department of Animal Life Science, Kangwon National University , Chuncheon, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Lee
authors.affiliation: 1 Department of Animal Life Science, Kangwon National University , Chuncheon, Korea.
authors.forename: Na Rae
authors.initials: NR
authors.lastname: Han
authors.affiliation: 2 Division of Applied Animal Science, Kangwon National University , Chuncheon, Korea.
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: 3 Division of Animal Resource Science, Kangwon National University , Chuncheon, Korea.
authors.forename: Jung Im
authors.initials: JI
authors.lastname: Yun
authors.affiliation: 4 Department of Neurosurgery, Kangwon National University Hospital, School of Medicine, Kangwon National University , Chuncheon, Korea.
authors.forename: Choonghyo
authors.initials: C
authors.lastname: Kim
authors.affiliation: 1 Department of Animal Life Science, Kangwon National University , Chuncheon, Korea.|3 Division of Animal Resource Science, Kangwon National University , Chuncheon, Korea.
authors.forename: Kyu Hyun
authors.initials: KH
authors.lastname: Park
authors.affiliation: 1 Department of Animal Life Science, Kangwon National University , Chuncheon, Korea.|2 Division of Applied Animal Science, Kangwon National University , Chuncheon, Korea.
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Lee
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005269:Femur / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005434:Flow Cytometry; D005780:Gelatin / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D003994:Bucladesine; D005780:Gelatin