title: [Levels of surfactant proteins A and D in bronchoalveolar lavage fluid of children with pneumonia and their relationships with clinical characteristics].
abstract: OBJECTIVE
  To observe the levels of pulmonary surfactant proteins A and D (SP-A, SP-D) in bronchoalveolar lavage fluid (BALF) of children with pneumonia, and to explore their relationships with clinical characteristics.
  
  
  METHODS
  Thirty-five children with pneumonia were enrolled in this study. Differential cell counts were obtained by Countstar counting board. The levels of SP-A and SP-D in BALF were detected using ELISA.
  
  
  RESULTS
  In children with pneumonia, SP-D levels were significantly higher than SP-A levels (P<0.001). SP-D levels were negatively correlated with the neutrophil percentage in BALF (r(s)=-0.5255, P<0.01). SP-D levels in BALF in children with increased blood C-reactive protein levels (>8 mg/L) were significantly lower than in those with a normal level of C-reactive protein (P<0.05). Compared with those in children without wheezing, SP-D levels in children with wheezing were significantly lower (P<0.01). There was no correlation between SP-A levels and clinical characteristics.
  
  
  CONCLUSIONS
  SP-D levels in BALF are significantly higher than SP-A levels, and have a certain correlation with clinical characteristics in children with pneumonia. As a protective factor, SP-D plays a more important role than SP-A in regulating the immune and inflammatory responses.
authors.affiliation: Pediatric Research Institute of Children's Hospital of Chongqing Medical University, Chongqing 400014, China. Emliu186@126.com.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Shou-Yan
authors.initials: SY
authors.lastname: Zheng
authors.forename: Luo
authors.initials: L
authors.lastname: Ren
authors.forename: Qiu-Yan
authors.initials: QY
authors.lastname: Xiao
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Long
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.forename: Qu-Bei
authors.initials: QB
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Deng
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Xie
authors.forename: En-Mei
authors.initials: EM
authors.lastname: Liu
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002097:C-Reactive Protein / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011014:Pneumonia / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis*; D037663:Pulmonary Surfactant-Associated Protein D / Q000032:analysis*
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D002097:C-Reactive Protein