title: Hematopoietic deletion of transferrin receptor 2 in mice leads to a block in erythroid differentiation during iron-deficient anemia.
abstract: Iron metabolism and erythropoiesis are inherently interlinked physiological processes. Regulation of iron metabolism is mediated by the iron-regulatory hormone hepcidin. Hepcidin limits the amount of iron released into the blood by binding to and causing the internalization of the iron exporter, ferroportin. A number of molecules and physiological stimuli, including erythropoiesis, are known to regulate hepcidin. An increase in erythropoietic demand decreases hepcidin, resulting in increased bioavailable iron in the blood. Transferrin receptor 2 (TFR2) is involved in the systemic regulation of iron metabolism. Patients and mice with mutations in TFR2 develop hemochromatosis due to inappropriate hepcidin levels relative to body iron. Recent studies from our laboratory and others have suggested an additional role for TFR2 in response to iron-restricted erythropoiesis. These studies used mouse models with perturbed systemic iron metabolism: anemic mice lacking matriptase-2 and Tfr2, or bone marrow transplants from iron-loaded Tfr2 null mice. We developed a novel transgenic mouse model which lacks Tfr2 in the hematopoietic compartment, enabling the delineation of the role of Tfr2 in erythroid development without interfering with its role in systemic iron metabolism. We show that in the absence of hematopoietic Tfr2 immature polychromatic erythroblasts accumulate with a concordant reduction in the percentage of mature erythroid cells in the spleen and bone marrow of anemic mice. These results demonstrate that erythroid Tfr2 is essential for an appropriate erythropoietic response in iron-deficient anemia. These findings may be of relevance in clinical situations in which an immediate and efficient erythropoietic response is required. Am. J. Hematol. 91:812-818, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Membrane Transport Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia.|School of Medicine, the University of Queensland, Brisbane, Queensland, 4006, Australia.
authors.forename: Gautam
authors.initials: G
authors.lastname: Rishi
authors.affiliation: Membrane Transport Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia.
authors.forename: Eriza S
authors.initials: ES
authors.lastname: Secondes
authors.affiliation: Membrane Transport Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Wallace
authors.affiliation: Membrane Transport Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia.|School of Medicine, the University of Queensland, Brisbane, Queensland, 4006, Australia.
authors.forename: V Nathan
authors.initials: VN
authors.lastname: Subramaniam
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000209:etiology / Q000473:pathology*; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D002454:Cell Differentiation; D041905:Erythroid Cells / Q000473:pathology; D004920:Erythropoiesis; D017353:Gene Deletion; D051379:Mice; D008822:Mice, Transgenic; D011990:Receptors, Transferrin / Q000235:genetics* / Q000502:physiology; D013154:Spleen / Q000473:pathology
chemical_list: D011990:Receptors, Transferrin; C503641:TFR2 protein, mouse