title: Novel electrochemical sensing platform for quantitative monitoring of Hg(II) on DNA-assembled graphene oxide with target recycling.
abstract: This work designs a new electrochemical sensing platform for the quantitative monitoring of mercury ion (Hg(2+)) on poly-T(15) oligonucleotide-functionalized graphene oxide by coupling with DNase I-assisted target recycling amplification. The assay was carried out on the basis of T-Hg(2+)-T coordination chemistry by using target-induced dissociation of indicator-labeled poly-T(15) oligonucleotide from graphene oxide nanosheets. The electronic signal was amplified through DNase I-triggered target recycling. Experimental results indicated that the amperometric response of DNA-based sensing platform deceased with the increasing Hg(2+) concentration in the sample, and has a detection limit of 0.12nM with a dynamic working range of up to 50nM. Our strategy afforded exquisite selectivity for Hg(2+) against other environmentally related metal ions. More significantly, this methodology displayed high reproducibility and acceptable accuracy, thus representing an optional sensing scheme for the screening of Hg(2+) in environmental water samples.
authors.affiliation: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China. Electronic address: minghualu88@yeah.net.
authors.forename: Minghua
authors.initials: M
authors.lastname: Lu
authors.affiliation: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Niu
authors.affiliation: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China.
authors.forename: Youmei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002413:Cations, Divalent / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D008958:Models, Molecular; D015203:Reproducibility of Results; D013941:Thymine / Q000737:chemistry; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D002413:Cations, Divalent; D014874:Water Pollutants, Chemical; D014867:Water; D006108:Graphite; D004247:DNA; D003850:Deoxyribonuclease I; D008628:Mercury; D013941:Thymine
keywords: DNase I; Electrochemical sensor; Graphene oxide; Mercury ion; Poly-T oligonucleotide; Target recycling