title: Spermatogenesis-associated proteins at different developmental stages of buffalo testicular seminiferous tubules identified by comparative proteomic analysis.
abstract: The testicular seminiferous tubules contain Sertoli cells and different types of spermatogenic cells. They provide the microenvironment for spermatogenesis, but the precise molecular mechanism of spermatogenesis is still not well known. Here, we have employed tandem mass tag coupled to LC-MS/MS with the high-throughput quantitative proteomics technology to explore the protein expression from buffalo testicular seminiferous tubules at three different developmental stages (prepuberty, puberty, and postpuberty). The results show 304 differentially expressed proteins with a ≥2-fold change, and bioinformatics analysis indicates that 27 of these may be associated with spermatogenesis. Expression patterns of seven selected proteins were verified via Western blot and quantitative RT-PCR analysis, and further cellular localizations of these proteins by immunohistochemical or immunofluorescence analysis. Taken together, the results provide potential molecular markers of spermatogenesis and provide a rich resource for further studies on male reproduction regulation.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Pan
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Fu-Mei
authors.initials: FM
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Huan-Jing
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Yang-Qing
authors.initials: YQ
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, P. R. China.|Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Nanning, Guangxi, P. R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D054758:A Kinase Anchor Proteins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002020:Buffaloes; D002853:Chromatography, Liquid; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D058977:Molecular Sequence Annotation; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012671:Seminiferous Tubules / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D012741:Sexual Maturation / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics*; D053719:Tandem Mass Spectrometry; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D054758:A Kinase Anchor Proteins; D006360:Heat-Shock Proteins; D020543:Proteome; D020741:rho GTP-Binding Proteins
keywords: Animal proteomics; Buffalo; Spermatogenesis-associated proteins; Tandem mass tag; Testicular seminiferous tubules