title: Synthesis of the Peptide Ac-Wahx-KTTKS and Evaluation of the Ability to Induce In Vitro Collagen Synthesis.
abstract: In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac- Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 µM for HaCaT and 500 µM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx- KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.
authors.forename: Danila A S
authors.initials: DA
authors.lastname: Guglielmi
authors.forename: Ariane M
authors.initials: AM
authors.lastname: Martinelli
authors.forename: Nathália C
authors.initials: NC
authors.lastname: Rissi
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Cilli
authors.forename: Christiane P
authors.initials: CP
authors.lastname: Soares
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP, Araraquara-Jaú Interstate Highway, Km 1, 14801-902 Araraquara, SP, Brazil. leila@fcfar.unesp.br.
authors.forename: Leila A
authors.initials: LA
authors.lastname: Chiavacci
mesh_terms: D000107:Acetylation; D045744:Cell Line, Tumor; D003094:Collagen / Q000096:biosynthesis / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D009842:Oligopeptides / Q000009:adverse effects / Q000138:chemical synthesis* / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects* / Q000473:pathology
chemical_list: D009842:Oligopeptides; C525923:lysyl-threonyl-threonyl-lysyl-serine; D003094:Collagen