title: Production of 3-D Airway Organoids From Primary Human Airway Basal Cells and Their Use in High-Throughput Screening.
abstract: The ability of human airway basal cells to serve as progenitor cells in the conducting airway makes them an attractive target in a number of respiratory diseases associated with epithelial remodeling. This unit describes a protocol for the culture of 'bronchospheres', three-dimensional (3-D) organoids that are derived from primary human airway basal cells. Mature bronchospheres are composed of functional multi-ciliated cells, mucin-producing goblet cells, and airway basal cells. In contrast to existing methods used for the culture of well-differentiated human airway epithelial cells, bronchospheres do not require growth on a permeable support and can be cultured in 384-well assay plates. The system provides a mechanism for investigating the regulation of basal cell fate during airway epithelial morphogenesis, as well as a basis for studying the function of the human airway epithelium in high-throughput assays. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts.
authors.forename: Marc
authors.initials: M
authors.lastname: Hild
authors.affiliation: Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts.
authors.forename: Aron B
authors.initials: AB
authors.lastname: Jaffe
mesh_terms: D001980:Bronchi / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D021621:Imaging, Three-Dimensional; D009693:Nucleic Acid Hybridization; D009940:Organoids / Q000166:cytology*; D012313:RNA / Q000302:isolation & purification; D015203:Reproducibility of Results; D018874:Spheroids, Cellular / Q000166:cytology; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D012313:RNA
keywords: bronchosphere; ciliated cell; goblet cell; high throughput screening; mucociliary