title: De novo assembly and annotation of the Antarctic copepod (Tigriopus kingsejongensis) transcriptome.
abstract: The whole transcriptome of the Antarctic copepod (Tigriopus kingsejongensis) was sequenced using Illumina RNA-seq. De novo assembly was performed with 64,785,098 raw reads using Trinity, which assembled into 81,653 contigs. TransDecoder found 38,250 candidate coding contigs which showed homology to other species by BLAST analysis. Functional gene annotation was performed by Gene Ontology (GO), InterProScan, and KEGG pathway analyses. Finally, we identified a number of expressed gene catalog for T. kingsejongensis that is a useful model animal for gene information-based polar research to uncover molecular mechanisms of environmental adaptation on harsh environments. In particular, we observed highly developing lipid metabolism in T. kingsejongensis directly compared to those of the Far East Pacific coast copepod Tigriopus japonicus at the transcriptome level.
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Hui-Su
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Lee
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Jeonghoon
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Inha University, Incheon 22212, South Korea.
authors.forename: Gi-Sik
authors.initials: GS
authors.lastname: Min
authors.affiliation: Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, South Korea. Electronic address: sangheekim@kopri.re.kr.
authors.forename: Sanghee
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address: jslee2@skku.edu.
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D033342:Copepoda / Q000235:genetics*; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D017423:Sequence Analysis, RNA; D059467:Transcriptome
keywords: Antarctic; Copepod; Lipid metabolism; RNA-seq; Tigriopus kingsejongensis; Transcriptome