title: Changes of MDA and SOD in Brain Tissue after Secondary Brain Injury with Seawater Immersion in Rats.
abstract: OBJECTIVE
  To investigate the variation and significance of malondialdehyde (MDA) and superoxide dismutase (SOD) in brain tissue after secondary brain injury (SBI) with seawater immersion in rats.
  
  
  METHODS
  We randomly divided 163 male Sprague Dawley rats into 4 groups, as normal (Group A), SBI (Group B), SBI with physiological saline immersion (Group C) and SBI with seawater immersion (Group D) groups. The animal model of ischemic SBI with seawater immersion was established based on the Marmarou's model of diffuse brain injury. The water content, and the MDA and SOD contents of brain tissue were detected at 1, 3, 6, 12, 24 and 48 hours after the injury.
  
  
  RESULTS
  Compared to group A, there were significant changes of various indicators in group D after injury at 1 hour after injury (P < 0.05). The water content and MDA contents in brain tissue were persistently elevated and significantly higher than that in groups B and C at each time phase (P < 0.05). The SOD content showed a persistent decline and was significantly lower than that in groups B and C at each time phase (P < 0.05). The SOD content was negatively correlated with the MDA content with a correlation coefficient of -0.992 (P < 0.01).
  
  
  CONCLUSIONS
  The SBI with seawater immersion is faster and more serious than the simple SBI.
authors.affiliation: Xiamen University, Fuzhou General Hospital, Department of Neurosurgery, Fuzhou, Fujian Province, China.
authors.forename: Liang-Feng
authors.initials: LF
authors.lastname: Wei
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Zhang
authors.forename: Shou-Sen
authors.initials: SS
authors.lastname: Wang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Jing
authors.forename: Zhao-Cong
authors.initials: ZC
authors.lastname: Zheng
authors.forename: Jin-Xi
authors.initials: JX
authors.lastname: Gao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001923:Brain Chemistry; D001930:Brain Injuries / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology*; D007101:Immersion / Q000503:physiopathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012623:Seawater; D013482:Superoxide Dismutase / Q000378:metabolism*; D014867:Water / Q000378:metabolism
chemical_list: D000975:Antioxidants; D014867:Water; D008315:Malondialdehyde; D013482:Superoxide Dismutase