title: Methyltransferase and demethylase profiling studies during brown adipocyte differentiation.
abstract: Although brown adipose tissue is important with regard to energy balance, the molecular mechanism of brown adipocyte differentiation has not been extensively studied. Specifically, regulation factors at the level of protein modification are largely unknown. In this study, we examine the changes in the expression level of enzymes which are involved in protein lysine methylation during brown adipocyte differentiation. Several enzymes, in this case SUV420H2, PRDM9, MLL3 and JHDM1D, were found to be up-regulated. On the other hand, Set7/9 was significantly down-regulated. In the case of SUV420H2, the expression level increased sharply during brown adipocyte differentiation, whereas the expression of SUV420H2 was marginally enhanced during the white adipocyte differentiation. The knock-down of SUV420H2 caused the suppression of brown adipocyte differentiation, as compared to a scrambled control. These results suggest that SUV420H2, a methyltransferase, is involved in brown adipocyte differentiation, and that the methylation of protein lysine is important in brown adipocyte differentiation. [BMB Reports 2016; 49(7): 388-393].
authors.affiliation: Metabolic Regulation Research Center, KRIBB, Daejeon 34141, Korea.
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Son
authors.affiliation: Metabolic Regulation Research Center, KRIBB; Department of Functional Genomics, University of Science and Technology (UST), Daejeon 34141, Korea.
authors.forename: Won Kon
authors.initials: WK
authors.lastname: Kim
authors.affiliation: Metabolic Regulation Research Center, KRIBB, Daejeon 34141, Korea.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Oh
authors.affiliation: Metabolic Regulation Research Center, KRIBB, Daejeon 34141, Korea.
authors.forename: Anna
authors.initials: A
authors.lastname: Park
authors.affiliation: Metabolic Regulation Research Center, KRIBB, Daejeon 34141, Korea.
authors.forename: Da Som
authors.initials: da S
authors.lastname: Lee
authors.affiliation: Metabolic Regulation Research Center, KRIBB; Department of Functional Genomics, University of Science and Technology (UST), Daejeon 34141, Korea.
authors.forename: Baek Soo
authors.initials: BS
authors.lastname: Han
authors.affiliation: Metabolic Regulation Research Center, KRIBB; Department of Functional Genomics, University of Science and Technology (UST), Daejeon 34141, Korea.
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Metabolic Regulation Research Center, KRIBB; Department of Functional Genomics, University of Science and Technology (UST), Daejeon 34141, Korea.
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
mesh_terms: D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D011495:Histone-Lysine N-Methyltransferase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism; D008745:Methylation; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000071256:Uncoupling Protein 1 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; C452979:KMT2C protein, human; D047495:PPAR gamma; D034741:RNA, Small Interfering; D000071256:Uncoupling Protein 1; D056484:Jumonji Domain-Containing Histone Demethylases; C548973:KDM7A protein, human; D011495:Histone-Lysine N-Methyltransferase; C542918:KMT5C protein, human; C544431:PRDM9 protein, human; C540312:SETD7 protein, human