title: Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes.
abstract: Our aim was to improve the developmental competence of bovine oocytes during their liquid storage by using additives. In vitro matured oocytes were stored for 20 h at 25°C in HEPES buffered TCM 199 medium (base medium). After storage, in vitro embryo development after in vitro fertilization was compared to those of non-stored (control) ones. Addition of 10% (v/v) newborn calf serum or 10.27 mmol/L pyruvate alone to the base medium did not improve blastocyst formation rates in stored oocytes; however, their simultaneous addition significantly improved the rate compared with those stored in base medium (P < 0.05). Supplementation of the holding medium with dithiothreitol (DTT) at any concentrations did not improve embryo development from stored oocytes. Although supplementation with cyclosporine A (CsA) significantly reduced apoptosis and membrane damage rates during storage, it did not improve the developmental competence of oocytes. 1,2-bis(2-aminophenoxy) ethane N,N,N',N'-tetraacetic acid tetrakis-acetoxymethyl ester and ruthenium red had no effect on oocyte apoptotic rates. Blastocyst formation rates in all stored groups remained significantly lower than that of the control. In conclusion, pyruvate and serum had a synergic effect to moderate the reduction of oocyte quality during storage, whereas mitochondrial membrane pore inhibitor CsA and the antioxidant DTT did not affect their developmental competence.
authors.affiliation: Embryo Technology and Stem Cell Research Center, Suranaree University of Technology, Nakhon Ratchasima, Thailand.|Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Tayita
authors.initials: T
authors.lastname: Suttirojpattana
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Tamas
authors.initials: T
authors.lastname: Somfai
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Matoba
authors.affiliation: Embryo Technology and Stem Cell Research Center, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
authors.forename: Rangsun
authors.initials: R
authors.lastname: Parnpai
authors.affiliation: Food and Fertilizer Technology Center, Taipei, Taiwan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagai
authors.affiliation: Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Geshi
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D016572:Cyclosporine / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects / Q000379:methods*; D006531:HEPES; D066298:In Vitro Techniques; D009865:Oocytes / Q000254:growth & development*; D019289:Pyruvic Acid / Q000494:pharmacology; D044967:Serum; D014021:Tissue Preservation
chemical_list: D003470:Culture Media; D016572:Cyclosporine; D019289:Pyruvic Acid; D006531:HEPES; D004229:Dithiothreitol
keywords: additives; bovine; in vitro fertilization; oocyte; storage