title: A signal amplification electrochemical aptasensor for the detection of breast cancer cell via free-running DNA walker.
abstract: Herein, a signal magnification electrochemical aptasensor for the detection of breast cancer cell via free-running DNA walker is constructed. Theoretically, just one DNA walker, released by target cell-responsive reaction, can automatically cleave all D-RNA (a chimeric DNA/RNA oligonucleotide with a cleavage point rArU) anchored on electrode into shorter produces, giving rise to considerably detectable signal finally. Under the optimal conditions, the electrochemical signal decreased linearly with the concentration of MCF-7 cell. The linear range is from 0 to 500 cells mL(-1) with a detection limit of 47 cellsmL(-1). In a word, this approach may have advantages over traditional reported DNA machines for bioassay, particularly in terms of ease of operation, cost efficiency, free of labeling and of complex track design, which may hold great potential for wide application.
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Cai
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
authors.forename: Yaokun
authors.initials: Y
authors.lastname: Xia
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian Province 350002, PR China.
authors.forename: Huanghao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China. Electronic address: cjh_huaxue@126.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D000328:Adult; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D021881:DNA, Catalytic / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D012313:RNA / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; C002612:DNA-like RNA; D012313:RNA
keywords: Breast cancer cell detection; DNA walker; Ease of operation; Electrochemical aptasensor; Signal amplification