title: [Transformation of icariin by immobilized β-glucosidase and snailase].
abstract: This study was performed to prepare immobilized β-glucosidase and snailase, then optimize and compare the process conditions for conversion of icariin. Immobilized β-glucosidase and snailase were prepared using crosslink-embedding method. The best conditions of the preparation process were optimized by single factor analysis and the properties of immobilized β-glucosidase and snailase were investigated. The reaction conditions including temperature, pH, substrate ratio, substrate concentration, reaction time and reusing times of the conversion of icariin using immobilized β-glucosidase or snailase were optimized. Immobilized β-glucosidase and snailase exhibited better heat stabilities and could remain about 60% activity after storage at 4 degrees C for 4 weeks. The optimized conditions for the conversion of icariin were as follows, the temperature of 50 degrees C, pH of 5.0, enzyme and substrate ratio of 1 : 1, substrate concentration of 0.1 mg x mL(-1), reaction time of 6 h for β-glucosidase and 2 h for snailase, respectively. In 5 experiments, the average conversion ratio of immobilized β-glucosidase and snailase was 70.76% and 74.97%. The results suggest an effect of promoted stabilities, prolonged lifetimes in both β-glucosidase and snailase after immobilization. The immobilized β-glucosidase and snailase exhibited a higher conversion rate and reusability compared to the free β-glucosidase and snailase. Moreover, the conversion rate of immobilized snailase was higher than that of immobilized β-glucosidase. The process of icariin conversion using immobilized β-glucosidase and snailase was moderate and feasible, which suggests that immobilized enzymes may hold a promise for industrial usage.
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.forename: Yi-hua
authors.initials: YH
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Cong-Yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D006868:Hydrolysis; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005419:Flavonoids; D001617:beta-Glucosidase; C056599:icariin