title: The effects of IGF1 on the melanogenesis in alpaca melanocytes in vitro.
abstract: In order to investigate the effects of the insulin-like growth factor 1(IGF-1) on alpaca melanocyte in vitro, different dosees of IGF1 (0, 10, 20, 40 ng/ml) were added in the medium of alpaca melanocyte. The RTCA machine was used to monitor the proliferation, quantitative real-time PCR, and western blot to test the relative gene expression, ELISA to test cAMP production, and spectrum method to test the melanin production. The results showed that compared to the normal melanocyte, the proliferation of melanocytes was increased within 60 h following adding IGF1. It also showed that cAMP content produced by melanocytes was increased, microphthalmia-associtated transcription factor (MITF), tyrosinase (TYR) and tyrosinase-related protein 2 (TYRP2) expression was increased, and melanin production with most obvious change in 10 ng/ml supplementary group, when compared with the control group. The results suggested that IGF1 with the dose of 10 ng/ml had the important effects on the melanogenesis in alpaca melanocyte by the cAMP pathway.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China.
authors.forename: Shuaipeng
authors.initials: S
authors.lastname: Hu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China.
authors.forename: Kaiyuan
authors.initials: K
authors.lastname: Ji
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China.
authors.forename: Xuexian
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China.
authors.forename: Junzhen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China. ruiwenfan@163.com.
authors.forename: Ruiwen
authors.initials: R
authors.lastname: Fan
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Shanxi, Taigu, 030801, China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Dong
mesh_terms: D000818:Animals; D002161:Camelids, New World; D000242:Cyclic AMP / Q000096:biosynthesis; D066298:In Vitro Techniques / Q000379:methods*; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects*; D053858:Metabolic Networks and Pathways / Q000187:drug effects
chemical_list: D008543:Melanins; D007334:Insulin-Like Growth Factor I; D000242:Cyclic AMP
keywords: Alpaca; IGF1; Melanocyte; Melanogenesis