title: Protective effects of maternal methyl donor supplementation on adult offspring of high fat diet-fed dams.
abstract: Obesity has become a global public health problem associated with metabolic dysfunction and chronic disorders. It has been shown that the risk of obesity and the DNA methylation profiles of the offspring can be affected by maternal nutrition, such as high-fat diet (HFD) consumption. The aim of this study was to investigate whether metabolic dysregulation and physiological abnormalities in offspring caused by maternal HFD can be alleviated by the treatment of methyl donors during pregnancy and lactation of dams. Female C57BL/6 mice were assigned to specific groups and given different nutrients (control diet, Control+Met, HFD and HFD+Met) throughout gestation and lactation. Offspring of each group were weaned onto a control diet at 3 weeks of age. Physiological (weight gain and adipose composition) and metabolic (plasma biochemical analyses) outcomes were assessed in male and female adult offspring. Expression and DNA methylation profiles of obesogenic-related genes including PPAR γ, fatty acid synthase, leptin and adiponectin were also detected in visceral fat of offspring. The results showed that dietary supplementation with methyl donors can prevent the adverse effects of maternal HFD on offspring. Changes in the expression and DNA methylation of obesogenic-related genes indicated that epigenetic regulation may contribute to the effects of maternal dietary factors on offspring outcomes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province 264003, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jiao
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province 264003, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, Guangdong Province 523808, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province 264003, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province 264003, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yue
authors.affiliation: Department of Biochemistry and Molecular Biology, Guizhou Medical University, Guiyang, Guizhou Province 550004, People's Republic of China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Ou
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province 264003, People's Republic of China; Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York, NY 10027, USA. Electronic address: ouhailong@gmc.edu.cn.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Yan
mesh_terms: D050154:Adiposity; D000818:Animals; D019175:DNA Methylation; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements; D005260:Female; D047109:Fetal Development; D018507:Gene Expression Regulation, Developmental; D050152:Intra-Abdominal Fat / Q000378:metabolism / Q000473:pathology; D007774:Lactation; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D008715:Methionine / Q000627:therapeutic use*; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000503:physiopathology; D063766:Pediatric Obesity / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000209:etiology / Q000503:physiopathology; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D014886:Weaning; D015430:Weight Gain
chemical_list: D008715:Methionine
keywords: Adipokine; DNA methylation; Epigenetic; High-fat diet; Methyl supplementation; Obesity