title: Use of the Hollow Fiber Assay to Evaluate Agents That Target the Tumor Neovasculature.
abstract: In vivo preclinical assays are required to screen potential agents that target the tumor vasculature. Here a hollow fiber-based assay for the quantification of neovasculature in the presence or absence of an agent that potentially targets tumor neovasculature is described. The neovasculature is developed as a consequence of the presence of tumor cells encapsulated in hollow fibers, which are transplanted sub-cutaneously in the dorsal flanks of mice.
authors.affiliation: Institute of Cancer Therapeutics, University of Bradford, Richmond Road, Bradford, BD7 1DP, UK.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Cooper
authors.affiliation: Institute of Cancer Therapeutics, University of Bradford, Richmond Road, Bradford, BD7 1DP, UK. S.D.Shnyder@Bradford.ac.uk.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Shnyder
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000098:blood supply / Q000188:drug therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents
keywords: Angiogenesis; CD-31; Cancer therapy; Hollow fiber assay; Preclinical screening; Tumor neovasculature; Vascular-targeting agents