title: MicroRNA-10b regulates epithelial-mesenchymal transition by modulating KLF4/Notch1/E-cadherin in cisplatin-resistant nasopharyngeal carcinoma cells.
abstract: Epithelial-mesenchymal transition (EMT) is an initiating event in tumor cell invasion and metastasis that contributes to therapeutic resistance to compounds including cisplatin. MicroRNAs (miRNAs) have been associated with EMT as well as resistance to standard therapies. However, the underlying mechanisms by which miRNAs control the development of resistance to cisplatin (DDP), and the accompanying EMT-like properties are required to elucidate. Here we show that microRNA-10b (miR-10b) is up-regulated in HNE1/DDP cells, and inhibition of miR-10b expression reversed the EMT phenotype. However, over-expression of miR-10b was able to promote the acquisition of an EMT phenotype in HNE1 cells. Additionally, we identified that miR-10b expression inversely correlates with KLF4, which then controls expression of Notch1. Knock-down of Notch1 inhibited cell migration, invasion, and reversed EMT in HNE1/DDP cells, which was dependent on miR-10b. In summary, our results reveal that miR-10b regulates EMT by modulating KLF4/Notch1/E-cadherin expression, which promotes invasion and migration of nasal pharyngeal carcinoma cells.
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals Bengbu 233030, Anhui, P. R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, The Fifth Affiliated Hospital of Sun Yat-sen University Zhuhai 519000, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Hong
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals Bengbu 233030, Anhui, P. R. China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Bengbu Medical College No 287, Chang-Huai Road, Bengbu 233000, Anhui, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of ENT, The First Affiliated Hospital of Bengbu Medical College No 287, Chang-Huai Road, Bengbu 233000, Anhui, P. R. China.
authors.forename: Shiyin
authors.initials: S
authors.lastname: Ma
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals Bengbu 233030, Anhui, P. R. China.
authors.forename: Surong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals Bengbu 233030, Anhui, P. R. China.
authors.forename: Mengxiao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical SchoolBoston, MA 02215, USA; The Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow UniversitySuzhou, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical PharmaceuticalsBengbu 233030, Anhui, P. R. China; Priority Research Center for Cancer Research, University of NewcastleNewcastle, NSW 2308, Australia.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals Bengbu 233030, Anhui, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
keywords: EMT; KLF4; Notch1; invasion; miR-10b; miRNA