title: The transcription factor SlDof22 involved in ascorbate accumulation and salinity stress in tomato.
abstract: Ascorbic acid (AsA) is an important antioxidant and its biosynthesis in plants has extensively been investigated. However, the key regulatory factors controlling the accumulation of AsA remain elusive. Here we report that tomato SlDof22, a member of the Dof family, negatively regulated AsA accumulation in tomato. RNA interference (RNAi) of SlDof22 in transgenic lines induced AsA levels, and affected the expression of genes in the D-mannose/L-galactose pathway and AsA recycling. In addition, SlSOS1 was significantly down-regulated in SlDof22 RNAi plants which resulted in reduced tolerance to salt stress. We further found that SlDof22 could bind to the promoter sequence of SlSOS1 gene by yeast one-hybrid analysis. Taken together, our data suggested that the Dof transcription factor SIDof22 involved in ascorbate accumulation and salt stress response in tomato.
authors.affiliation: Development and Collaborative Innovation Center of Plant Germplasm Resources, College of Life and Environment Science, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cai
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Shu
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Hanxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: yyzhang@mail.hzau.edu.cn.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D001205:Ascorbic Acid
keywords: Ascorbic acid; Dof protein; Salt overly sensitive; Tomato; Transcription factor