title: Absence of chromosomal translocations and protein expression of ALK in sinonasal adenocarcinomas.
abstract: BACKGROUND
  Chromosomal translocations at 2p23 cause overexpression of anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase involved in signalling pathways that regulate cell proliferation. This translocation occurs in 5% of lung adenocarcinoma and has been demonstrated to be useful as a therapeutic target for crizotinib. sinonasal adenocarcinomas (SNAC) are histologically similar to lung adenocarcinomas; the aim of this study was to evaluate the presence of ALK alterations in SNAC.
  
  
  METHODS
  Break-apart fluorescent in-situ hybridization was used to analyse the presence of ALK translocations in 96 tumour samples. In addition, ALK protein expression was studied by immunohistochemistry.
  
  
  RESULTS
  The samples of SNAC did not show ALK translocation. Moreover, ALK protein expression was absent in all cases.
  
  
  CONCLUSIONS
  These results suggest that ALK is not involved in SNAC.
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: Esteban
authors.initials: E
authors.lastname: Pacheco
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España. Electronic address: llorentependas@telefonica.net.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Llorente
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: Alejandro
authors.initials: A
authors.lastname: López-Hernández
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: Cristina
authors.initials: C
authors.lastname: García-Inclán
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: María
authors.initials: M
authors.lastname: Costales
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: Sira
authors.initials: S
authors.lastname: Potes Ares
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: Fernando
authors.initials: F
authors.lastname: López
authors.affiliation: Departamento de Anatomía Patológica, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: Blanca
authors.initials: B
authors.lastname: Vivanco
authors.affiliation: Departamento de Otorrinolaringología, Instituto Universitario de Oncología del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo, Asturias, España.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Hermsen
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D000077548:Anaplastic Lymphoma Kinase; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics / Q000648:ultrastructure*; D000077547:Crizotinib; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009669:Nose Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010255:Paranasal Sinus Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011720:Pyrazoles; D011725:Pyridines; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D009363:Neoplasm Proteins; D011720:Pyrazoles; D011725:Pyridines; D000077547:Crizotinib; C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases
keywords: ALK; Adenocarcinoma nasosinusal; Carcinoma nasosinusal; FISH; Sinonasal adenocarcinoma; Sinonasal carcinoma