title: Evaluation of the antidiabetic and antioxidant properties of Morinda lucida stem bark extract in streptozotocin intoxicated rats.
abstract: The present research evaluated the antidiabetic and antioxidant properties of M. lucida stem bark (50 and 500mg/kg) and glibenclamide (25mg/kg, standard drug) in acute (Oral glucose tolerance test) and sub-acute (Streptozotocin 60mg/kg, i.p. diabetic model) administration. A group of healthy rats constituted the normal control. The sub-acute experiment lasted 28 days during which water, food intake and weight gain were measured and biochemical parameters analyzed in both plasma and erythrocytes at the end of the experiment. The chemical substances present in M. lucida bark extract were determined. In the Oral glucose tolerance test, the reduction of blood glucose level was statistically significant for both M. lucida extracts and glibenclamide. However, in the diabetic rats acute administration of 500mg/kg extract had better blood sugar lowering effect than glibenclamide, which was better than 50mg/kg extract. Streptozotocin diabetic animal model was characterized by a decrease in weight gain, erythrocyte SOD and CAT activities and an increase in water and food consumption, lipid peroxidation, cholesterol, triglycerides, plasma glucose, creatinine and urea concentrations, and transaminases activities. M. lucida extract and glibenclamide significantly prevented the alteration of these parameters, thus indicating a corrective effect on diabetes and its complications. This study justifies the traditional claim and provides a rationale for the use of M. lucida to treat diabetes. Its antioxidant properties may serve to curb oxidative stress and hence prevent the diabetic complications related to oxidative stress. Chemical substances, which may be accountable for the antidiabetic and antioxidant properties of M. lucida were detected in the aqueous extract of M. lucida bark.
authors.affiliation: Department of Animal Biology and Physiology, University of Yaoundé I, Cameroon.
authors.forename: Ulrich L F
authors.initials: UL
authors.lastname: Domekouo
authors.affiliation: Laboratoire de Physiologie Animale de l'Ecole Normale Supérieure, Université de Yaoundé I, Cameroun.
authors.forename: Frida
authors.initials: F
authors.lastname: Longo
authors.affiliation: Centre for the Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroun.
authors.forename: Protus A
authors.initials: PA
authors.lastname: Tarkang
authors.affiliation: Centre for the Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroun.
authors.forename: Alembert T
authors.initials: AT
authors.lastname: Tchinda
authors.affiliation: Centre for the Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroun.
authors.forename: Nole
authors.initials: N
authors.lastname: Tsabang
authors.affiliation: Centre for the Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroun.
authors.forename: Nehemie T
authors.initials: NT
authors.lastname: Donfagsiteli
authors.affiliation: Centre for the Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroun.
authors.forename: Victorine
authors.initials: V
authors.lastname: Tamze
authors.affiliation: Department of Animal Biology and Physiology, University of Yaoundé I, Cameroon.
authors.forename: Pierre
authors.initials: P
authors.lastname: Kamtchouing
authors.affiliation: Centre for the Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroun.
authors.forename: Gabriel A
authors.initials: GA
authors.lastname: Agbor
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005951:Glucose Tolerance Test; D005905:Glyburide / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D032066:Morinda / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D013311:Streptozocin; D013997:Time Factors
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D008055:Lipids; D010936:Plant Extracts; D013311:Streptozocin; D005905:Glyburide