title: Probiotic properties of lactic acid bacteria isolated from human milk.
abstract: OBJECTIVE
  The objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.
  
  
  RESULTS
  The strains were tested for their ability to inhibit growth of Enterococcus faecalis, Salmonella enterica subsp. enterica serotype Enteritidis, Listeria monocytogenes, Staphylococcus aureus and Escherichia coli, as well as for susceptibility to antimicrobial agents and for acid pH and bile salt tolerance. Gram-positive and catalase-negative were selected and identified as Enterococcus (83·3%) after sequencing the 16S rDNA gene. All the isolates inhibited growth of Ent. faecalis and S. serotype Enteritidis, 97% inhibited growth of L. monocytogenes and Staph. aureus and 78·8% inhibited growth of E. coli. Most of the isolates were resistant to gentamicin (50%) and vancomycin (47%). Twelve isolates grew when subjected to pH 3·0 and 0·1% bile salts. At lower pH (2·5-2·0), Ent. faecalis F1 and Weissella confusa F8 were more efficient.
  
  
  CONCLUSIONS
  It was possible to isolate from human milk the lactic acid bacteria with potential for use as probiotics.
  
  
  CONCLUSIONS
  Lactic acid bacteria isolated of nursing mothers have probiotic properties.
authors.affiliation: Center of Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Cruz das Almas, Brazil.
authors.forename: N A
authors.initials: NA
authors.lastname: Reis
authors.affiliation: Nucleus of Research in Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Brazil.
authors.forename: M A F
authors.initials: MA
authors.lastname: Saraiva
authors.affiliation: Center of Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Cruz das Almas, Brazil.
authors.forename: E A A
authors.initials: EA
authors.lastname: Duarte
authors.affiliation: Center of Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Cruz das Almas, Brazil.
authors.forename: E A
authors.initials: EA
authors.lastname: de Carvalho
authors.affiliation: Center of Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Cruz das Almas, Brazil.
authors.forename: B B
authors.initials: BB
authors.lastname: Vieira
authors.affiliation: Center of Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo da Bahia, Cruz das Almas, Brazil.
authors.forename: N S
authors.initials: NS
authors.lastname: Evangelista-Barreto
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008895:Milk, Human / Q000382:microbiology*; D019936:Probiotics / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D019344:Lactic Acid
keywords: Enterococcus; antagonism; human milk; lactic acid bacteria; probiotics