title: Comparison of Zirconium Phosphonate-Modified Surfaces for Immobilizing Phosphopeptides and Phosphate-Tagged Proteins.
abstract: Different routes for preparing zirconium phosphonate-modified surfaces for immobilizing biomolecular probes are compared. Two chemical-modification approaches were explored to form self-assembled monolayers on commercially available primary amine-functionalized slides, and the resulting surfaces were compared to well-characterized zirconium phosphonate monolayer-modified supports prepared using Langmuir-Blodgett methods. When using POCl3 as the amine phosphorylating agent followed by treatment with zirconyl chloride, the result was not a zirconium-phosphonate monolayer, as commonly assumed in the literature, but rather the process gives adsorbed zirconium oxide/hydroxide species and to a lower extent adsorbed zirconium phosphate and/or phosphonate. Reactions giving rise to these products were modeled in homogeneous-phase studies. Nevertheless, each of the three modified surfaces effectively immobilized phosphopeptides and phosphopeptide tags fused to an affinity protein. Unexpectedly, the zirconium oxide/hydroxide modified surface, formed by treating the amine-coated slides with POCl3/Zr(4+), afforded better immobilization of the peptides and proteins and efficient capture of their targets.
authors.affiliation: Chimie et Interdisciplinarité: Synthèse Analyse Modélisation (CEISAM), Université de Nantes, CNRS, UMR 6230 , 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Forato
authors.affiliation: Department of Chemistry, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: CRMD-CNRS, 1B rue de la férollerie, 45071 Orléans Cedex 2, France.
authors.forename: Roland
authors.initials: R
authors.lastname: Benoit
authors.affiliation: CNRS, CEMHTI UPR3079, Université de Orléans , F-45071 Orléans, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Fayon
authors.affiliation: Fonctionnalité et Ingénierie des Protéines (UFIP), Université de Nantes, CNRS, UMR 6286 , 2 rue de la Houssinière BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Cathy
authors.initials: C
authors.lastname: Charlier
authors.affiliation: Fonctionnalité et Ingénierie des Protéines (UFIP), Université de Nantes, CNRS, UMR 6286 , 2 rue de la Houssinière BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Amina
authors.initials: A
authors.lastname: Fateh
authors.affiliation: Fonctionnalité et Ingénierie des Protéines (UFIP), Université de Nantes, CNRS, UMR 6286 , 2 rue de la Houssinière BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Defontaine
authors.affiliation: Fonctionnalité et Ingénierie des Protéines (UFIP), Université de Nantes, CNRS, UMR 6286 , 2 rue de la Houssinière BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Tellier
authors.affiliation: Department of Chemistry, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Talham
authors.affiliation: Chimie et Interdisciplinarité: Synthèse Analyse Modélisation (CEISAM), Université de Nantes, CNRS, UMR 6230 , 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Clémence
authors.initials: C
authors.lastname: Queffélec
authors.affiliation: Chimie et Interdisciplinarité: Synthèse Analyse Modélisation (CEISAM), Université de Nantes, CNRS, UMR 6230 , 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Bujoli
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D016945:Sulfolobus acidocaldarius / Q000737:chemistry*; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry*
chemical_list: D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; D063065:Organophosphonates; D010748:Phosphopeptides; D010750:Phosphoproteins; C095116:Sac7 protein, Sulfolobus; D015040:Zirconium