title: Hydatid cyst fluid promotes peri-cystic fibrosis in cystic echinococcosis by suppressing miR-19 expression.
abstract: Echinococcus granulosus infection causes cystic echinococcosis (CE); the generation of liver fibrosis around the parasitic larval cyst (metacestode) may play a major role in the spontaneous limitation of the parasitic growth; however, fibrogenesis has received little attention in CE. It has been reported that miR-19b plays a role in various diseases, including infectious diseases, by regulating fibrogenesis. However, its function in the development of liver fibrosis in E. granulosus infection is unknown.
  
  
  
  The expression of miR-19b and genes that are involved in liver fibrosis were analysed in E. granulosus-infected human livers using qRT-PCR. The role of miR-19b on hepatic stellate cells (LX-2 cells in vitro) treated with hydatid cyst fluid (HCF) was then analysed by 3-(4, 5-dimet-hylthiazol-2-yl)-2, 4-diphenyl-tetrazolium bromide (MTT) assay, qRT-PCR, Western blot and flow cytometry.
  
  
  
  The results showed that the expression of miR-19 was significantly reduced in the pericystic collagen-rich liver tissue of CE patients, compared to normal liver. Incubation of LX-2 cells (in vitro) with HCF induced a decreased proliferation of these cells and a reduced expression of miR-19, inversely correlated with the expression of collagen 1A1 and TGF-β receptor II (TβRII). Conversely, overexpression of miR-19 by LX-2 cells inhibited the proliferation of these cells and led to decreased TβRII expression.
  
  
  
  Our study provides new evidence for the intervention of miRNAs in the regulation of fibrosis in infectious diseases; it suggests that E. granulosus can inhibit miR-19 liver expression and promote fibrosis through the increase in TβRII, the activation of hepatic stellate cells and extracellular matrix production.
authors.affiliation: Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Chuanshan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890298), The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Tuergan
authors.initials: T
authors.lastname: Ali
authors.affiliation: Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Bi
authors.affiliation: Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Lü
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890298), The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Shao
authors.affiliation: WHO-Collaborating Centre for the Prevention and Treatment of Human Echinococcosis, Department of Parasitology, University of Franche-Comté (EA 3181) and University Hospital, Besançon, France.
authors.forename: Dominique A
authors.initials: DA
authors.lastname: Vuitton
authors.affiliation: State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890298), The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. Dr.wenhao@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Wen
authors.affiliation: Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. renyong_lin@sina.com.
authors.forename: Renyong
authors.initials: R
authors.lastname: Lin
mesh_terms: D000328:Adult; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020420:Cyst Fluid / Q000502:physiology; D003550:Cystic Fibrosis / Q000469:parasitology*; D004443:Echinococcosis / Q000469:parasitology*; D004446:Echinococcus / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000469:parasitology*; D008297:Male; D035683:MicroRNAs / Q000187:drug effects* / Q000235:genetics; D008875:Middle Aged; D055815:Young Adult
chemical_list: C549214:MIRN19A microRNA, human; D035683:MicroRNAs
keywords: Echinococcus granulosus; HSC; Liver fibrosis; TβRII; miR-19