title: ADAMTS4 and Oxidative/Antioxidative Status in Preterm Premature Rupture of Membranes.
abstract: OBJECTIVE
  To determine the function of a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS4), total oxidant status (TOS), total antioxidant status (TAS), and aryl esterase (ARES) in preterm premature rupture of membranes (PPROM) and to investigate the association with premature rupture of membranes (PROMs).
  
  
  METHODS
  58 pregnant women were included in this prospective study which comprised 29 PPROM patients as the study group and 29 patients, having healthy amniotic membranes, as the control group. ADAMTS4, TAS, TOS, and ARES levels were studied in the amniotic membrane homogenates of the patients.
  
  
  RESULTS
  ADAMTS4, TAS TOS, and ARES levels of amniotic membrane lysates were significantly different between PPROM and control groups (p < 0.001, p < 0.001, p = 0.008 and p = 0.002, respectively). Increased amniotic membrane ADAMTS4 (OR: 1.051 95% CI 1.006-1.098, p = 0.024) and TOS (OR: 12.777 95% CI 1.595-102.323, p = 0.016) were found to be significantly associated with the increased risk of PPROM.
  
  
  CONCLUSIONS
  ADAMTS4, TOS, and ARES levels were higher and TAS level was lower in PPROM patients than the normal healthy control group which had healthy amniotic membranes at term. As a result, ADAMTS4 may have a role in the pathogenesis by causing increased oxidative and inflammatory environment in PPROM.
authors.affiliation: a Department of Perinatology , Zekai Tahir Burak Women's Health Education and Research Hospital , Ankara , Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Ozler
authors.affiliation: a Department of Perinatology , Zekai Tahir Burak Women's Health Education and Research Hospital , Ankara , Turkey.
authors.forename: Efser
authors.initials: E
authors.lastname: Oztas
authors.affiliation: b Department of Obstetrics and Gynecology, Liv Hospital , Ankara , Turkey.
authors.forename: Basak Gumus
authors.initials: BG
authors.lastname: Guler
authors.affiliation: c Department of Biochemistry , Gaziantep 25th Aralik State Hospital , Gaziantep , Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Ergin
authors.affiliation: a Department of Perinatology , Zekai Tahir Burak Women's Health Education and Research Hospital , Ankara , Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Uygur
authors.affiliation: a Department of Perinatology , Zekai Tahir Burak Women's Health Education and Research Hospital , Ankara , Turkey.
authors.forename: Aykan
authors.initials: A
authors.lastname: Yucel
authors.affiliation: d Department of Clinical Biochemistry , Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey.
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
authors.affiliation: a Department of Perinatology , Zekai Tahir Burak Women's Health Education and Research Hospital , Ankara , Turkey.
authors.forename: Nuri
authors.initials: N
authors.lastname: Danisman
mesh_terms: D000071121:ADAMTS4 Protein / Q000032:analysis / Q000096:biosynthesis*; D000328:Adult; D000650:Amnion / Q000378:metabolism*; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D047928:Premature Birth / Q000209:etiology / Q000378:metabolism; D011446:Prospective Studies
chemical_list: D000975:Antioxidants; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human
keywords: ADAMTS4; ARES; PPROM; TAS; TOS; amniotic membranes