title: Flow cytometry community fingerprinting and amplicon sequencing for the assessment of landfill leachate cellulolytic bioaugmentation.
abstract: Flow cytometry (FCM) is a high throughput single cell technology that is actually becoming widely used for studying phenotypic and genotypic diversity among microbial communities. This technology is considered in this work for the assessment of a bioaugmentation treatment in order to enhance cellulolytic potential of landfill leachate. The experimental results reveal the relevant increase of leachate cellulolytic potential due to bioaugmentation. Cytometric monitoring of microbial dynamics along these assays is then realized. The flow FP package is used to establish microbial samples fingerprint from initial 2D cytometry histograms. This procedure allows highlighting microbial communities' variation along the assays. Cytometric and 16S rRNA gene sequencing fingerprinting methods are then compared. The two approaches give same evidence about microbial dynamics throughout digestion assay. There are however a lack of significant correlation between cytometric and amplicon sequencing fingerprint at genus or species level. Same phenotypical profiles of microbiota during assays matched to several 16S rRNA gene sequencing ones. Flow cytometry fingerprinting can thus be considered as a promising routine on-site method suitable for the detection of stability/variation/disturbance of complex microbial communities involved in bioprocesses.
authors.affiliation: University of Liège, Gembloux Agro-Bio Tech, Microbial Processes and Interactions (MiPI), Passage des déportés 2, Gembloux, B-5030, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: Kinet
authors.affiliation: University of Liège, Gembloux Agro-Bio Tech, Microbial Processes and Interactions (MiPI), Passage des déportés 2, Gembloux, B-5030, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Dzaomuho
authors.affiliation: University of Liège, Gembloux Agro-Bio Tech, Microbial Processes and Interactions (MiPI), Passage des déportés 2, Gembloux, B-5030, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Baert
authors.affiliation: Fundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine, University of Liège, Sart-Tilman, B43b, Liège B-4000, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Taminiau
authors.affiliation: Fundamental and Applied Research for Animal & Health (FARAH), Food Science Department, Faculty of Veterinary Medicine, University of Liège, Sart-Tilman, B43b, Liège B-4000, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Daube
authors.affiliation: Quality Partner S.A., Rue Hayeneux, 62, Herstal, B-4040, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Nezer
authors.affiliation: Computer Science and Modeling, Applied Statistics, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2, Gembloux, B-5030, Belgium.
authors.forename: Y
authors.initials: Y
authors.lastname: Brostaux
authors.affiliation: University of Liege, Applied Geophysics, Department ArGEnCo, Engineering Faculty, B52, B-4000 Liege, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Nguyen
authors.affiliation: University of Liege, Applied Geophysics, Department ArGEnCo, Engineering Faculty, B52, B-4000 Liege, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Dumont
authors.affiliation: Artechno S.A., Rue Herman Méganck, 21, Isnes, B-5032, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Thonart
authors.affiliation: University of Liège, Gembloux Agro-Bio Tech, Microbial Processes and Interactions (MiPI), Passage des déportés 2, Gembloux, B-5030, Belgium. Electronic address: f.delvigne@ulg.ac.be.
authors.forename: F
authors.initials: F
authors.lastname: Delvigne
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D002482:Cellulose / Q000032:analysis*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000502:physiology; D004784:Environmental Monitoring; D005434:Flow Cytometry; D005784:Gene Amplification / Q000502:physiology; D017422:Sequence Analysis, DNA; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; D014874:Water Pollutants, Chemical; D002482:Cellulose
keywords: Amplicon sequencing; Anaerobic digestion; Bioaugmentation; Cellulose; Flow cytometry; Landfill; Leachate