title: Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case-control study and meta-analysis.
abstract: Tumor suppressor p53 is a critical player in the fight against cancer as it controls the cell cycle check point, apoptotic pathways and genomic stability. It is known to be the most frequently mutated gene in a wide variety of human cancers. Single-nucleotide polymorphism of p53 at codon72 leading to substitution of proline (Pro) in place of arginine (Arg) has been identified as a risk factor for development of many cancers, including nasopharyngeal carcinoma (NPC). However, the association of this polymorphism with NPC across the published literature has shown conflicting results. We aimed to conduct a case-control study for a possible relation of p53 codon72 Arg>Pro polymorphism with NPC risk in underdeveloped states of India, combine the result with previously available records from different databases and perform a meta-analysis to draw a more definitive conclusion. A total of 70 NPC patients and 70 healthy controls were enrolled from different hospitals of north-eastern India. The p53 codon72 Arg>Pro polymorphism was typed by polymerase chain reaction, which showed an association with NPC risk. In the meta-analysis consisting of 1842 cases and 2330 controls, it was found that individuals carrying the Pro allele and the ProPro genotype were at a significantly higher risk for NPC as compared with those with the Arg allele and the ArgArg genotype, respectively. Individuals with a ProPro genotype and a combined Pro genotype (ProPro+ArgPro) also showed a significantly higher risk for NPC over a wild homozygote ArgArg genotype. Additionally, the strength of each study was tested by power analysis and genotype distribution by Hardy-Weinberg equilibrium. The outcome of the study indicated that both allele frequency and genotype distribution of p53 codon72 Arg>Pro polymorphism were significantly associated with NPC risk. Stratified analyses based on ethnicity and source of samples supported the above result.
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Sahu
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: S
authors.initials: S
authors.lastname: Chakrabarti
authors.affiliation: Department of Radiation Oncology, Civil Hospital, Dimapur, Nagaland, India.
authors.forename: S D
authors.initials: SD
authors.lastname: Roy
authors.affiliation: Dr B. Borooah Cancer Institute, ENT Department, Guwahati, Assam, India.
authors.forename: N
authors.initials: N
authors.lastname: Baishya
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: R R
authors.initials: RR
authors.lastname: Reddy
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: S
authors.initials: S
authors.lastname: Suklabaidya
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: S
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: S
authors.initials: S
authors.lastname: Maji
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: A
authors.initials: A
authors.lastname: Suryanwanshi
authors.affiliation: Department of Biotechnology, Regional Medical Research Centre for Tribals, Jabalpur, India.
authors.forename: S
authors.initials: S
authors.lastname: Rajasubramaniam
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: M
authors.initials: M
authors.lastname: Asthana
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.|Central University of Jharkhand, Brambe, Ranchi, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Panda
authors.affiliation: Shri Ramachandra Bhanj Medical College, Cuttack, Department of Gastroentrology, Odisha, India.
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Siksha Vhabana, Visva Bharati, Santiniketan, Bolpur, India.
authors.forename: S
authors.initials: S
authors.lastname: Ganguly
authors.affiliation: Department of Biotechnology, Siksha Vhabana, Visva Bharati, Santiniketan, Bolpur, India.
authors.forename: O P
authors.initials: OP
authors.lastname: Shaw
authors.affiliation: Department of Biotechnology, Siksha Vhabana, Visva Bharati, Santiniketan, Bolpur, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Bichhwalia
authors.affiliation: Department of Biotechnology, Siksha Vhabana, Visva Bharati, Santiniketan, Bolpur, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Sahoo
authors.affiliation: Department of Biotechnology, Siksha Vhabana, Visva Bharati, Santiniketan, Bolpur, India.
authors.forename: N R
authors.initials: NR
authors.lastname: Chattopadhyay
authors.affiliation: Department of Biotechnology, Siksha Vhabana, Visva Bharati, Santiniketan, Bolpur, India.
authors.forename: K
authors.initials: K
authors.lastname: Chatterjee
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.|School of Biotechnology, KIIT University, Bhubaneswar, India.
authors.forename: C N
authors.initials: CN
authors.lastname: Kundu
authors.affiliation: Dr B. Borooah Cancer Institute, ENT Department, Guwahati, Assam, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Das
authors.affiliation: Cachar Cancer Hospital & Research Centre, Silchar, Assam, India.
authors.forename: R
authors.initials: R
authors.lastname: Kannan
authors.affiliation: Civil Hospital, Aizawl, Mizoram, India.
authors.lastname: Zorenpuii
authors.affiliation: Civil Hospital, Aizawl, Mizoram, India.
authors.forename: E
authors.initials: E
authors.lastname: Zomawia
authors.affiliation: Department of Radiation Oncology, Civil Hospital, Dimapur, Nagaland, India.
authors.forename: S A
authors.initials: SA
authors.lastname: Sema
authors.affiliation: Regional Institute of Medical Sciences, Department of Radiotherapy, Imphal, Manipur, India.
authors.forename: Y I
authors.initials: YI
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Assam Central University, Silchar, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Ghosh
authors.affiliation: Guwahati Medical College & Hospital, Guwahati, Assam, India.
authors.forename: K
authors.initials: K
authors.lastname: Sharma
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Das
authors.affiliation: Division of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.|Department of Biotechnology, Siksha Vhabana, Visva Bharati, Santiniketan, Bolpur, India.
authors.forename: T
authors.initials: T
authors.lastname: Choudhuri