title: Vioserpin, a serine protease inhibitor from Gloeobacter violaceus possibly regulated by heparin.
abstract: Serine peptidase inhibitor (serpin) is the name given to the superfamily of proteins with wide range of biological functions, and that the main feature is the inhibition of serine proteases. Here we describe the inhibitory characterization of a serpin from Gloeobacter violaceus that we named vioserpin. The serpin presented a high specificity to inhibit trypsin-like enzymes with a rapid inhibition rate constant (2.1 × 10(6) M(-1) s(-1)). We also demonstrated that the inhibitory activity of the vioserpin is influenced by the concentration of heparin, and this finding may throw a new light on understanding the molecular evolution of serpins.
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Jocélia P C
authors.initials: JP
authors.lastname: Oliveira
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Natália
authors.initials: N
authors.lastname: Salazar
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Zani
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Lucas R
authors.initials: LR
authors.lastname: de Souza
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Passos
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Aquiles M
authors.initials: AM
authors.lastname: Sant'Ana
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Regiane A
authors.initials: RA
authors.lastname: de Andrade
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saude Animal, Universidade de São Paulo, São Paulo, SP, Brazil; Medicina Veterinária e Bem Estar Animal, Universidade de Santo Amaro, Rua Prof. Enéas de Siqueira Neto, 340, São Paulo, SP, Brazil.
authors.forename: Arlei
authors.initials: A
authors.lastname: Marcili
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil.
authors.forename: Marcia A
authors.initials: MA
authors.lastname: Sperança
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus 3, São Bernardo do Campo, SP, Brazil. Electronic address: puzer@ufabc.edu.br.
authors.forename: Luciano
authors.initials: L
authors.lastname: Puzer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D000458:Cyanobacteria; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D051379:Mice; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D006493:Heparin; D014357:Trypsin
keywords: Bacterial serpin; Gloeobacter violaceus; Protease inhibitor; Serine peptidase; Serpin; Vioserpin