title: Investigation of Hemoglobin/Gold Nanoparticle Heterolayer on Micro-Gap for Electrochemical Biosensor Application.
abstract: In the present study, we fabricated a hemoglobin/gold nanoparticle (Hb/GNP) heterolayer immobilized on the Au micro-gap to confirm H₂O₂ detection with a signal-enhancement effect. The hemoglobin which contained the heme group catalyzed the reduction of H₂O₂. To facilitate the electron transfer between hemoglobin and Au micro-gap electrode, a gold nanoparticle was introduced. The Au micro-gap electrode that has gap size of 5 µm was fabricated by conventional photolithographic technique to locate working and counter electrodes oppositely in a single chip for the signal sensitivity and reliability. The hemoglobin was self-assembled onto the Au surface via chemical linker 6-mercaptohexanoic acid (6-MHA). Then, the gold nanoparticles were adsorbed onto hemoglobin/6-MHA heterolayers by the layer-by-layer (LbL) method. The fabrication of the Hb/GNP heterolayer was confirmed by atomic force microscopy (AFM) and surface-enhanced Raman spectroscopy (SERS). The redox property and H₂O₂ detection of Hb/GNP on the micro-gap electrode was investigated by a cyclic voltammetry (CV) experiment. Taken together, the present results show that the electrochemical signal-enhancement effect of a hemoglobin/nanoparticle heterolayer was well confirmed on the micro-scale electrode for biosensor applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea. nanotlee@gmail.com.|Research Center for Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea. nanotlee@gmail.com.
authors.forename: Taek
authors.initials: T
authors.lastname: Lee
authors.affiliation: School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 156-756, Korea. thkim0512@gmail.com.
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea. iverson@naver.com.
authors.forename: Jinho
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Chemical Engineering, Hoseo University, 20, Hoseo-ro 79beon-gil, Baebang-Eup, Asan City, Chungnam 336-795, Korea. yhchung@hoseo.edu.
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Chung
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea. jylee72@sogang.ac.kr.|Research Center for Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea. jylee72@sogang.ac.kr.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea. jwchoi@sogang.ac.kr.|Research Center for Integrated Biotechnology, Sogang University, 35 Baekbeom-ro (Sinsu-dong), Mapo-gu, Seoul 121-742, Korea. jwchoi@sogang.ac.kr.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold; D006454:Hemoglobins / Q000032:analysis*; D006861:Hydrogen Peroxide; D053768:Metal Nanoparticles; D053758:Nanoparticles; D015203:Reproducibility of Results
chemical_list: D006454:Hemoglobins; D006046:Gold; D006861:Hydrogen Peroxide
keywords: Au micro-gap; cyclic voltammetry; electrochemical biosensor; gold nanoparticle; hemoglobin