title: '2A-Like' Signal Sequences Mediating Translational Recoding: A Novel Form of Dual Protein Targeting.
abstract: We report the initial characterization of an N-terminal oligopeptide '2A-like' sequence that is able to function both as a signal sequence and as a translational recoding element. Owing to this translational recoding activity, two forms of nascent polypeptide are synthesized: (i) when 2A-mediated translational recoding has not occurred: the nascent polypeptide is fused to the 2A-like N-terminal signal sequence and the fusion translation product is targeted to the exocytic pathway, and, (ii) a translation product where 2A-mediated translational recoding has occurred: the 2A-like signal sequence is synthesized as a separate translation product and, therefore, the nascent (downstream) polypeptide lacks the 2A-like signal sequence and is localized to the cytoplasm. This type of dual-functional signal sequence results, therefore, in the partitioning of the translation products between the two sub-cellular sites and represents a newly described form of dual protein targeting.
authors.affiliation: Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, Scotland, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Roulston
authors.affiliation: Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, Scotland, UK.
authors.forename: Garry A
authors.initials: GA
authors.lastname: Luke
authors.affiliation: Spanish Medicines Agency (AEMPS), Parque Empresarial "Las Mercedes", Campezo 1 - Edificio 8, 28022, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: de Felipe
authors.affiliation: Oakland Innovation, Harston Mill, Harston, Cambridge, CB22 7GG, UK.
authors.forename: Lin
authors.initials: L
authors.lastname: Ruan
authors.affiliation: James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cope
authors.affiliation: Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, Scotland, UK.
authors.forename: John
authors.initials: J
authors.lastname: Nicholson
authors.affiliation: Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, Scotland, UK.
authors.forename: Andriy
authors.initials: A
authors.lastname: Sukhodub
authors.affiliation: Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, Scotland, UK.
authors.forename: Jens
authors.initials: J
authors.lastname: Tilsner
authors.affiliation: Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, Scotland, UK.
authors.forename: Martin D
authors.initials: MD
authors.lastname: Ryan
mesh_terms: D006801:Humans; D009842:Oligopeptides / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology*; D021382:Protein Sorting Signals / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology*; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; D021382:Protein Sorting Signals
keywords: 2A; dual protein targeting; secretory pathway; signal sequence; translational recoding