title: Friedmanniella endophytica sp. nov., an endophytic actinobacterium isolated from bark of Kandelia candel.
abstract: A coccus-shaped, non-spore-forming actinobacterium, designated strain 4Q3S-3T, was isolated from surface-sterilized bark of the mangrove plant Kandelia candel collected from Cotai Ecological Zones in Macao, China, and tested by a polyphasic approach to clarify its taxonomic position. This actinobacterium was Gram-stain-positive and aerobic. Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested. Strain 4Q3S-3T grew optimally without NaCl at 28-30 °C, pH 7.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain 4Q3S-3T belonged to the genus Friedmanniella and shared the highest 16S rRNA gene sequence similarity with Friedmanniellaflava W6T (96.57 %). The DNA G+C content of strain 4Q3S-3T was 69.5 mol%. The cell-wall peptidoglycan contained ll-2,6-diaminopimelic acid, and MK-9(H4) was the predominant menaquinone. The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, unidentified glycolipid, amino lipids and two unidentified phospholipids. The predominant fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. Based on phylogenetic, phenotypic and chemotaxonomic data, strain 4Q3S-3T represents a novel species of the genus Friedmanniella, for which the name Friedmanniella endophytica sp. nov. is proposed. The type strain is 4Q3S-3T (=DSM 100723T=CGMCC 4.7307T).
authors.affiliation: Research Center for Medicine & Biology, Zunyi Medical University, Zunyi 563003, PR China.|Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Tuo
authors.affiliation: Research Center for Medicine & Biology, Zunyi Medical University, Zunyi 563003, PR China.|Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Fei-Na
authors.initials: FN
authors.lastname: Li
authors.affiliation: Faculty of Science and Technology, Department of Civil and Environmental Engineering, University of Macau, Macao, PR China.
authors.forename: Inchio
authors.initials: I
authors.lastname: Lou
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
authors.forename: Simon Ming-Yuen
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Cheng-Hang
authors.initials: CH
authors.lastname: Sun
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D043982:Rhizophoraceae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid