title: In Vitro Generation of Human NK Cells Expressing Chimeric Antigen Receptor Through Differentiation of Gene-Modified Hematopoietic Stem Cells.
abstract: NK cells represent a very promising source for adoptive cellular approaches for cancer immunotherapy, and extensive research has been conducted, including clinical trials. Gene modification of NK cells can direct their specificity and enhance their function, but the efficiency of gene transfer techniques is very limited. Here we describe two protocols designed to generate mature human NK cells from gene-modified hematopoietic stem cells. These protocols use chimeric antigen receptor as the transgene, but could potentially be modified for the expression any particular transgene in human NK cells.
authors.affiliation: Department of Microbiology, Immunology & Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Lowe
authors.affiliation: Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.|Division of Hematology/Oncology, Mattel Children's Hospital-UCLA Medical Center, Los Angeles, CA, USA.
authors.forename: Laurel C
authors.initials: LC
authors.lastname: Truscott
authors.affiliation: Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. sdeoliveira@mednet.ucla.edu.|Division of Hematology/Oncology, Mattel Children's Hospital-UCLA Medical Center, Los Angeles, CA, USA. sdeoliveira@mednet.ucla.edu.
authors.forename: Satiro N
authors.initials: SN
authors.lastname: De Oliveira
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D018014:Gene Transfer Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D011946:Receptors, Antigen / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: D011946:Receptors, Antigen; D011993:Recombinant Fusion Proteins
keywords: Gene transfer; Hematopoietic stem cells; Lentiviral vector; NK cells; OP9 cell line