title: Selective solid-phase extraction of catecholamines from plasma using nanofibers doped with crown ether and their quantitation by HPLC with electrochemical detection.
abstract: A simple and convenient preparation method has been developed for polymeric crown ethers containing the dibenzo-18-crown-6 subunit and electrospun composite nanofibers composed of polymeric crown ethers with polystyrene (PCE-PS). Furthermore, this composite nanofiber was used as a sorbent for selective extraction of plasma catecholamines, viz. dopamine (DA), norepinephrine (NE), and epinephrine (E). After the protein deposition of the plasma sample, the supernatant was adjusted to neutral pH with buffer solution. Then, the mixture was loaded and pushed through a column packed with composite nanofiber sorbent, and the analytes were eluted with 50 μL of acetic acid. The effectiveness of the plasma sample cleanup method was verified by high-performance liquid chromatography with electrochemical detection (HPLC-ECD). The PCE-PS packed column may prove clinically useful, as it provides a convenient and selective method for evaluation of human plasma catecholamines. Graphical Abstract Scheme showing the selective packed fiber SPE of catecholamines from spiked plasma using nanofibers doped with ploymeric crown ether.
authors.affiliation: School of Public Health, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: LiQin
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: XingHua
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Public Health, Tianjin Medical University, Tianjin, 300070, China. shenjun@tmu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.affiliation: School of Public Health, Tianjin Medical University, Tianjin, 300070, China. zhangwanqi@tmu.edu.cn.
authors.forename: WanQi
authors.initials: W
authors.lastname: Zhang
mesh_terms: D065966:Absorption, Physicochemical; D001774:Blood Chemical Analysis / Q000379:methods; D002395:Catecholamines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D043844:Crown Ethers / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D002395:Catecholamines; D043844:Crown Ethers
keywords: Packed fiber solid-phase extraction; Plasma catecholamine; Polymeric crown ether