title: Purification of Pyridylaminated Oligosaccharides Using 1,2-Dichloroethane Extraction.
abstract: Fluorescence derivatization of the oligosaccharides released from glycoconjugates is widely used for precise structural characterization. To ensure labeling of the oligosaccharides, a large excess of fluorescence reagents is usually added to the reaction tube. Therefore, any excess reagents and by-products of the labeling reaction should be removed by several column chromatographies, including using a cellulose cartridge or spin columns. However, these purification steps are often time-consuming, expensive, and laborious. In this study, we found that 1,2-dichloroethane extraction could effectively and easily purify pyridylaminated oligosaccharides with a high recovery rate.
authors.affiliation: College of Science and Technology, Nihon University.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Aya
authors.initials: A
authors.lastname: Okano
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kabayama
authors.forename: Atsuyoshi
authors.initials: A
authors.lastname: Nishina
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanigawa
authors.forename: Katsushi
authors.initials: K
authors.lastname: Nishimura
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kushi