title: Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats.
abstract: OBJECTIVE
  This study aimed to investigate the difference among the expression of serotonin receptors (5-HT3, 5-HT4, and 5-HT7receptors) in colonic tissue of chronic diarrhea rats.
  
  
  METHODS
  A rat model of chronic diarrhea was established by lactose diet. The expression of 5-HT3, 5-HT4, and 5-HT7receptors in the colonic tissue was detected using immunohistochemistry, real-time PCR and Western blotting techniques.
  
  
  RESULTS
  There is no significant difference on the protein expression of 5-HT3receptor between the normal group and the chronic diarrhea model group. The mRNA expression of 5-HT3receptor in the chronic diarrhea model group was significantly lower than that in the normal group (n = 10; P< 0.01). The protein and mRNA expression of 5-HT4receptor in the chronic diarrhea model group were significantly higher than those in the normal group (n = 10; P< 0.05, P< 0.01). On the contrary, the protein and mRNA expressions of 5-HT7receptor in the chronic diarrhea model group were significantly decreased compared with the normal group (n = 10; P< 0.01, P< 0.01).
  
  
  CONCLUSIONS
  The results suggested the receptors of 5-HT4and 5-HT7may be involved in inducing diarrhea by lactose diet.
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Gastroenterology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Huishu
authors.initials: H
authors.lastname: Guo
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D007785:Lactose / Q000008:administration & dosage; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011985:Receptors, Serotonin; D007785:Lactose