title: Nanostructured glycopolymer augmented liposomes to elucidate carbohydrate-mediated targeting.
abstract: Carbohydrate receptors on alveolar macrophages are attractive targets for receptor-mediated delivery of nanostructured therapeutics. In this study, we employed reversible addition fragmentation chain transfer polymerization to synthesize neoglycopolymers, consisting of mannose- and galactose methacrylate-based monomers copolymerized with cholesterol methacrylate for use in functional liposome studies. Glycopolymer-functional liposomes were employed to elucidate macrophage mannose receptor (CD206) and macrophage galactose-type lectin (CD301) targeting in both primary macrophage and immortal macrophage cell lines. Expression of CD206 and CD301 was observed to vary significantly between cell lines (murine alveolar macrophage, murine bone marrow-derived macrophage, RAW264.7, and MH-S), which has significant implications in in vitro targeting and uptake studies. Synthetic glycopolymers and glycopolymer augmented liposomes demonstrated specific receptor-mediated uptake in a manner dependent on carbohydrate receptor expression. These results establish a platform capable of probing endogenous carbohydrate receptor-mediated targeting via glycofunctional nanomaterials.
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA.
authors.forename: Hye-Nam
authors.initials: HN
authors.lastname: Son
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Hill
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA.
authors.forename: Selvi
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA.
authors.forename: Fang-Yi
authors.initials: FY
authors.lastname: Su
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Stayton
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Convertine
authors.affiliation: Department of Bioengineering, The University of Washington, Seattle, WA, USA. Electronic address: dratner@uw.edu.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Ratner
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002460:Cell Line; D004337:Drug Carriers; D006801:Humans; D037102:Lectins; D008081:Liposomes; D008264:Macrophages; D016676:Macrophages, Alveolar; D008358:Mannose; D051379:Mice
chemical_list: D004337:Drug Carriers; D037102:Lectins; D008081:Liposomes; D008358:Mannose
keywords: Carbohydrate; Glycopolymer; Liposome; Macrophage