title: Optimising selective excitation pulses to maximise saturation transfer difference NMR spectroscopy.
abstract: A simple method is presented that optimizes the STD NMR Gaussian pulse to deliver significant increases in STD amplification factors with minimal perturbation of the ligand. This approach is practically demonstrated using the wheat-germ agglutinin/N-acetyl-D-glucosamine protein-ligand system.
authors.affiliation: School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK.
authors.forename: Nathan B
authors.initials: NB
authors.lastname: Ley
authors.affiliation: School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Rowe
authors.affiliation: School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Williamson
authors.affiliation: School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Howard