title: High-Resolution Vertical Observation of Intracellular Structure Using Magnetically Responsive Microplates.
abstract: A vertical confocal observation system capable of high-resolution observation of intracellular structure is demonstrated. The system consists of magnet-active microplates to rotate, incline, and translate single adherent cells in the applied magnetic field. Appended to conventional confocal microscopes, this system enables high-resolution cross-sectional imaging with single-molecule sensitivity in single scanning.
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Teshima
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onoe
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Tottori
authors.affiliation: Department of Tropical Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105-8461, Japan.
authors.forename: Hiroka
authors.initials: H
authors.lastname: Aonuma
authors.affiliation: Graduate School of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan.
authors.forename: Takeomi
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Kanagawa Academy of Science and Technology, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa, 213-0012, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Kamiya
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ishihara
authors.affiliation: Department of Tropical Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105-8461, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanuka
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D004867:Equipment Design; D006801:Humans; D008280:Magnetics; D018613:Microscopy, Confocal / Q000295:instrumentation*
keywords: confocal microscopy; cytoskeletons; magnetic materials; parylene; single cell analysis