title: Post-transcriptional gene silencing activity of human GIGYF2.
abstract: In mammalian post-transcriptional gene silencing, the Argonaute protein AGO2 indirectly recruits translation inhibitors, deadenylase complexes, and decapping factors to microRNA-targeted mRNAs, thereby repressing mRNA translation and accelerating mRNA decay. However, the exact composition and assembly pathway of the microRNA-induced silencing complex are not completely elucidated. As the GYF domain of human GIGYF2 was shown to bind AGO2 in pulldown experiments, we wondered whether GIGYF2 could be a novel protein component of the microRNA-induced silencing complex. Here we show that full-length GIGYF2 coimmunoprecipitates with AGO2 in human cells, and demonstrate that, upon tethering to a reporter mRNA, GIGYF2 exhibits strong, dose-dependent silencing activity, involving both mRNA destabilization and translational repression.
authors.affiliation: UMR8113 LBPA, ENS Cachan, CNRS, Université Paris-Saclay, 94230, Cachan, France. Electronic address: kryszke@ens-cachan.fr.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Kryszke
authors.affiliation: UMR8113 LBPA, ENS Cachan, CNRS, Université Paris-Saclay, 94230, Cachan, France. Electronic address: badiaadjiriou34@hotmail.com.
authors.forename: Badia
authors.initials: B
authors.lastname: Adjeriou
authors.affiliation: UMR8113 LBPA, ENS Cachan, CNRS, Université Paris-Saclay, 94230, Cachan, France. Electronic address: fl@biomon.fr.
authors.forename: Feifei
authors.initials: F
authors.lastname: Liang
authors.affiliation: UMR8113 LBPA, ENS Cachan, CNRS, Université Paris-Saclay, 94230, Cachan, France. Electronic address: hong.chen@u-psud.fr.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: UMR8113 LBPA, ENS Cachan, CNRS, Université Paris-Saclay, 94230, Cachan, France. Electronic address: fdautry@ens-cachan.fr.
authors.forename: François
authors.initials: F
authors.lastname: Dautry
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D020868:Gene Silencing; D017930:Genes, Reporter; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D014176:Protein Biosynthesis; D060066:Protein Interaction Maps; D020871:RNA Stability; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; D002352:Carrier Proteins; C478016:GIGYF2 protein, human; D012333:RNA, Messenger
keywords: Argonaute 2; GIGYF2; Post-transcriptional gene silencing; RNA-induced silencing complex; TNRC6