title: IgA response and protection following nasal vaccination of chickens with Newcastle disease virus DNA vaccine nanoencapsulated with Ag@SiO2 hollow nanoparticles.
abstract: Newcastle disease caused by ND virus (NDV) is a highly contagious disease of birds. Vaccine for effective protection of poultry animals from NDV infection is urgently needed. Mucosal immunity plays a very important role in the antiviral immune response. In this study, a NDV F gene-containing DNA vaccine encapsulated in Ag@SiO2 hollow nanoparticles (pFDNA-Ag@SiO2-NPs) with an average diameter of 500 nm were prepared to assess the mucosal immune response. These nanoparticles exhibited low cytotoxicity and did not destroy the bioactivity of plasmid DNA, which could be expressed in vitro. The plasmid DNA was sustainably released after an initial burst release. In vivo immunization showed that the intranasal immunization of chickens with pFDNA-Ag@SiO2-NPs induced high titers of serum antibody, significantly promoted lymphocyte proliferation and induced higher expression levels of IL-2 and IFN-γ in a dose-dependent manner. These results indicated that the Ag@SiO2 hollow nanoparticles could serve as an efficient and safe delivery carrier for NDV DNA vaccine to induce mucosal immunity. This study has provided promising results for the further development of mucosal vaccines encapsulated in inorganic nanoparticles.
authors.affiliation: School of Biological Science and Technology, University of Jinan, Jinan 250022, P. R.China.|Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, P. R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, P. R. China.|Department of Avian Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Rong
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, P. R. China.|Department of Avian Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Kang
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, P. R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, Laboratory of Physical Chemistry, Key Laboratory of Chemical Engineering Process &Technology for High-efficiency Conversion, School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, P. R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, Laboratory of Physical Chemistry, Key Laboratory of Chemical Engineering Process &Technology for High-efficiency Conversion, School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, P. R. China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Department of Avian Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Li
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D002645:Chickens; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007376:Interleukin-2; D019444:Vaccines, DNA; D014765:Viral Vaccines; D012834:Silver; D012822:Silicon Dioxide; D007371:Interferon-gamma