title: Lipidomic Profiling of Adipose Tissue Reveals an Inflammatory Signature in Cancer-Related and Primary Lymphedema.
abstract: Cancer-related and primary lymphedema (LE) are associated with the production of adipose tissue (AT). Nothing is known, however, about the lipid-based molecules that comprise LE AT. We therefore analyzed lipid molecules in lipoaspirates and serum obtained from LE patients, and compared them to lipoaspirates from cosmetic surgery patients and healthy control cohort serum. LE patient serum analysis demonstrated that triglycerides, HDL- and LDL-cholesterol and lipid transport molecules remained within the normal range, with no alterations in individual fatty acids. The lipidomic analysis also identified 275 lipid-based molecules, including triacylglycerides, diacylglycerides, fatty acids and phospholipids in AT oil and fat. Although the majority of lipid molecules were present in a similar abundance in LE and non-LE samples, there were several small changes: increased C20:5-containing triacylglycerides, reduced C10:0 caprinic and C24:1 nervonic acids. LE AT oil also contained a signature of increased cyclopropane-type fatty acids and inflammatory mediators arachidonic acid and ceramides. Interestingly C20:5 and C22:6 omega-3-type lipids are increased in LE AT, correlating with LE years. Hence, LE AT has a normal lipid profile containing a signature of inflammation and omega-3-lipids. It remains unclear, however, whether these differences reflect a small-scale global metabolic disturbance or effects within localised inflammatory foci.
authors.affiliation: Department of Clinical Medicine, Faculty of Medicine & Health Science, Macquarie University, Sydney, NSW, Australia.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Sedger
authors.affiliation: Metabolomics Australia, Bio21 Institute, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Dedreia L
authors.initials: DL
authors.lastname: Tull
authors.affiliation: Metabolomics Australia, Bio21 Institute, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: McConville
authors.affiliation: Metabolomics Australia, Bio21 Institute, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: David P
authors.initials: DP
authors.lastname: De Souza
authors.affiliation: Metabolomics Australia, Bio21 Institute, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Thusitha W T
authors.initials: TW
authors.lastname: Rupasinghe
authors.affiliation: Metabolomics Australia, Bio21 Institute, The University of Melbourne, Melbourne, VIC, Australia.|School of Chemistry, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Spencer J
authors.initials: SJ
authors.lastname: Williams
authors.affiliation: Metabolomics Australia, Bio21 Institute, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Dayalan
authors.affiliation: Daniel Lanzer Clinic, Malvern, Melbourne, VIC, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lanzer
authors.affiliation: Macquarie University Hospital, North Ryde, Sydney, NSW, Australia.
authors.forename: Helen
authors.initials: H
authors.lastname: Mackie
authors.affiliation: Macquarie University Hospital, North Ryde, Sydney, NSW, Australia.
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Lam
authors.affiliation: Department of Clinical Medicine, Faculty of Medicine & Health Science, Macquarie University, Sydney, NSW, Australia.|Macquarie University Hospital, North Ryde, Sydney, NSW, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Boyages
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001692:Biological Transport; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood*; D008209:Lymphedema / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism*; D008297:Male; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D009369:Neoplasms / Q000150:complications*; D010743:Phospholipids / Q000378:metabolism; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D005227:Fatty Acids; D018836:Inflammation Mediators; D008055:Lipids; D010743:Phospholipids