title: Testicular transcript responses in rare minnow Gobiocypris rarus following different concentrations bisphenol A exposure.
abstract: Bisphenol A (BPA) is widely spread in the environment. It can cause various reproductive disrupting effects on different organisms, including fish. To investigate the effect of BPA at different concentrations comprehensively, RNA-seq was performed on the testicular mRNA libraries of adult male rare minnow Gobiocypris rarus that exposed to 0, 1, 15 and 225 μg/L BPA for 7 days. Meanwhile, biological indicators and sex steroid hormone levels were investigated. Result showed that (1) BPA at all three concentrations affected the expression of genes related to testicular steroid hormone biosynthesis, blood-testis barrier, proteolysis, and lipid transport and metabolism. (2) BPA at 1 μg/L induced gene expression in renin-angiotensin system pathway and possibly initiate membrane form of estrogen receptor (mER); 1 and 15 μg/L BPA inhibited tRNA processing-related genes expression; 15 and 225 μg/L BPA decreased hemostasis and blood coagulation-related gene expression. The present study indicated that BPA did influence rare minnow testicular gene expressing, and the effect BPA effects varied with concentration.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Jiancao
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China. Electronic address: zzwang@nwsuaf.edu.cn.
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D003530:Cyprinidae / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000235:genetics*; D008297:Male; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics; D012084:Renin-Angiotensin System / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D012739:Gonadal Steroid Hormones; D010636:Phenols; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; RNA-seq; Rare minnow Gobiocypris rarus; Sex steroid hormone; Testis