title: Combination of Serum Interleukin-1β and 6 Levels in the Diagnosis of Perinatal Asphyxia.
abstract: BACKGROUND
  Perinatal asphyxia is an important cause of death, as well as permanent neurological and developmental complications. Diagnosing in time would lead to better prognosis and applying the most proper treatment. We sought to define the predictive values of serum concentrations of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in newborns with perinatal asphyxia to see if there is a relation between the short-term neurological deficit and serum IL-1β and IL-6 concentrations.
  
  
  METHODS
  This was a prospective (case-control) study conducted between March 2006 and April 2013, at the Neonatal Intensive Care Unit, Mashhad, Iran. Serum IL-1β and IL-6 levels were measured at birth in 38 consecutive uninfected neonates with perinatal asphyxia (blood pH < 7.2, low Apgar score, signs of fetal distress) and 47 randomly selected healthy newborns. The results were compared between the groups, using Chi-Square, t-tests, and Mann-Whitney tests, as well as receiver operator characteristics (ROC) curves and regression models.
  
  
  RESULTS
  Serum IL-1β and IL-6 concentrations in the infants who developed perinatal asphyxia were significantly higher compared to values in the normal infants [16.88 vs  3.34 pg/mL for IL-1β, (P = 0.006), and 88.15 vs 6.74 pg/ mL for IL-6, (P < 0.001) respectively]. The sensitivity and  specificity for the diagnosis of perinatal asphyxia using serum IL-6 were 80.5% and 81.6% respectively. The sensitivity and specificity using serum IL-1β were 71% and 89.1%, respectively.
  
  
  CONCLUSIONS
  Evaluating serum IL-6 and 1β simultaneously, could improve the sensitivity and specificity of early diagnosis of the perinatal  asphyxia. The most appropriate indicator of perinatal asphyxia is combined measurement of interleukin 1β and interleukin 6.
authors.affiliation: Department of Pediatrics, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Boskabadi
authors.affiliation: Department of Pediatrics, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.
authors.forename: Gholamali
authors.initials: G
authors.lastname: Maamouri
authors.affiliation: Department of Immunology, Immunology Research Center, Avicenna Research Institute, Mashhad, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Tavakkol Afshari
authors.affiliation: Department of Midwifery, School of Medicine, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran. maryamzakerihamidi@yahoo.co.nz.
authors.forename: Maryam
authors.initials: M
authors.lastname: Zakerihamidi
authors.affiliation: Neonatal Intensive Care Nursing, Faculty of Nursing, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Kalateh Molaee
authors.affiliation: Neonatal Intensive Care Nursing, Faculty of Nursing, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Bagheri
authors.affiliation: Biochemistry and Nutrition Research Center and Cardiovascular Research Center, Faculty of Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Parizadeh
authors.affiliation: Biochemistry and Nutrition Research Center and Cardiovascular Research Center, Faculty of Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Ghayour-Mobarhan
authors.affiliation: Orthopedic Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Moradi
authors.affiliation: Division of Medical Education, Brighton and Sussex Medical School, Mayfield House, Falmer, BN19PH, UK.
authors.forename: Gordon A A
authors.initials: GA
authors.lastname: Ferns
mesh_terms: D001034:Apgar Score; D001238:Asphyxia Neonatorum / Q000097:blood* / Q000175:diagnosis*; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal / Q000458:organization & administration*; D053583:Interleukin-1beta / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D007492:Iran; D008297:Male; D011446:Prospective Studies; D012372:ROC Curve; D012044:Regression Analysis; D012680:Sensitivity and Specificity
chemical_list: C508585:IL1B protein, human; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6