title: Polymorphism in the A2M gene associated with high-quality milk in Murrah buffaloes (Bubalus bubalis).
abstract: The study of genes associated with host defense mechanisms, such as the A2M gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. The aim of this study was to identify polymorphisms in the A2M gene in Murrah buffaloes (Bubalus bubalis), and investigate their associations with milk yield, fat and protein production, fat and protein percentages, and somatic cell count. Hair follicle samples of 136 animals were collected for DNA extraction, and polymorphisms were identified by polymerase chain reactions and sequencing. Statistical analyses were performed to ascertain the allelic and genotypic frequencies, the Hardy-Weinberg equilibrium, and association analysis was conducted between the polymorphisms and the traits studied. Comparative analysis between buffalo and bovine sequences revealed seven nucleotide substitutions. Alignments among the buffalo sequences identified three single nucleotide polymorphisms (SNPs), including one in exon 29, g.241A>G, which was used in subsequent statistical analyses. A Hardy-Weinberg test indicated that this SNP was in equilibrium in this population, and was significantly associated (P < 0.05) with fat production and fat and protein percentages. Therefore, this SNP can be used as a molecular marker for these traits.
authors.forename: A C
authors.initials: AC
authors.lastname: Freitas
authors.affiliation: Universidade Tecnológica Federal do Paraná, Dois Vizinhos, PR, Brasil.
authors.forename: G M F
authors.initials: GM
authors.lastname: de Camargo
authors.forename: R R
authors.initials: RR
authors.lastname: Aspilcueta-Borquis
authors.forename: N B
authors.initials: NB
authors.lastname: Stafuzza
authors.affiliation: Departamento de Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, SP, Brasil.
authors.forename: G C
authors.initials: GC
authors.lastname: Venturini
authors.forename: F
authors.initials: F
authors.lastname: Tanamati
authors.forename: N A
authors.initials: NA
authors.lastname: Hurtado-Lugo
authors.forename: C C
authors.initials: CC
authors.lastname: Barros
authors.forename: H
authors.initials: H
authors.lastname: Tonhati
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D005260:Female; D063427:Food Quality; D007774:Lactation / Q000235:genetics; D008297:Male; D008892:Milk / Q000378:metabolism* / Q000592:standards; D020641:Polymorphism, Single Nucleotide; D000511:alpha-Macroglobulins / Q000235:genetics*
chemical_list: D000511:alpha-Macroglobulins