title: Binding Sites in the EFG1 Promoter for Transcription Factors in a Proposed Regulatory Network: A Functional Analysis in the White and Opaque Phases of Candida albicans.
abstract: In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.
authors.affiliation: Developmental Studies Hybridoma Bank, Department of Biology, University of Iowa, Iowa 52242.
authors.forename: Claude
authors.initials: C
authors.lastname: Pujol
authors.affiliation: Developmental Studies Hybridoma Bank, Department of Biology, University of Iowa, Iowa 52242.
authors.forename: Thyagarajan
authors.initials: T
authors.lastname: Srikantha
authors.affiliation: Developmental Studies Hybridoma Bank, Department of Biology, University of Iowa, Iowa 52242.
authors.forename: Yang-Nim
authors.initials: YN
authors.lastname: Park
authors.affiliation: Developmental Studies Hybridoma Bank, Department of Biology, University of Iowa, Iowa 52242.
authors.forename: Karla J
authors.initials: KJ
authors.lastname: Daniels
authors.affiliation: Developmental Studies Hybridoma Bank, Department of Biology, University of Iowa, Iowa 52242 david-soll@uiowa.edu.
authors.forename: David R
authors.initials: DR
authors.lastname: Soll
mesh_terms: D000483:Alleles; D001665:Binding Sites; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D017930:Genes, Reporter; D009154:Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C121438:EFG1 protein, Candida albicans; D005656:Fungal Proteins; D014157:Transcription Factors
keywords: cis-acting sequences; differential gene expression; white-opaque transition