title: Functional polymorphisms of the cyclooxygenase-2 gene and prognosis of hepatocellular carcinoma - a cohort study in Chinese people.
abstract: Cyclooxygenase-2 (COX-2) influences carcinogenesis through regulation of angiogenesis, apoptosis, cytokine expression, and immune response suppression. It has been well established that COX-2 is overexpressed in a variety of human cancers, such as hepatocellular carcinoma (HCC). In this study, we aimed to evaluate the association between COX-2 polymorphisms and prognosis of HCC. We genotyped 200 HCC patients of Chinese Han descent for COX-2 gene polymorphisms (-765G>C and -1195G>A) using PCR-RFLP. Data were statistically analyzed using the Kaplan-Meier method and the Cox's proportional hazard regression model. We found that patients with the COX-2 -1195AG and -1195AG + AA genotypes demonstrated significantly decreased disease-free survival (DFS) as compared with those carrying the -1195GG genotype (P < 0.05). However, the COX-2 -765G>C polymorphism was not associated with DFS (P > 0.05). Moreover, by Cox regression analysis, blood alpha fetoprotein ≤400 ng/mL before the operation and the -1195G>A polymorphism were found to be of prognostic significance (P < 0.05), while the -765G>C polymorphism was not (P > 0.05). In summary, post-operation progression of HCC is more likely to occur in patients with the -1195AG genotype and the A allele. On the other hand, the -765G>C polymorphism is not an independent influence factor of HCC prognosis.
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: R
authors.initials: R
authors.lastname: Liang
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Yan
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Yuan
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: First Department of Chemotherapy, Affiliated Tumour Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics*; D002681:China; D015331:Cohort Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis
chemical_list: C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2