title: Sulfation pattern of fucose branches affects the anti-hyperlipidemic activities of fucosylated chondroitin sulfate.
abstract: Fucosylated chondroitin sulfates (fCSs) are glycosaminoglycans extracted from sea cucumbers, consisting of chondroitin sulfate E (CSE) backbones and sulfated fucose branches. The biological properties of fCSs could be affected by the sulfation pattern of their fucose branches. In the present study, two fCSs were isolated from sea cucumbers Isostichopus badionotus (fCS-Ib) and Pearsonothuria graeffei (fCS-Pg). Their monosaccharide compositions of glucuronic acid (GlcA), N-acetylgalactosamine (GalNAc), fucose (Fuc) and sulfate were at similar molar ratio with 1.0/0.7/0.9/3.1 for fCS-Ib and 1.0/0.8/1.5/2.6 for fCS-Pg. The two fCSs have different sulfation patterns on their fucose branches, fCS-Pg with 3,4-O-disulfation while fCS-Ib with 2,4-O-disulfation. Their antihyperlipidemic effects were compared using a high-fat high-fructose diet (HFFD)-fed C57BL/6J mice model. Both fCS-Ib and fCS-Pg had significant effects on lipid profile improvement, liver protection, blood glucose diminution and hepatic glycogen synthesis. Specifically, fCS-Pg with 3,4-O-disulfation fucose branches was more effective in reduction of blood cholesterol (TC), low density lipoprotein (LDL) and atherogenic index (AI). Our results indicate that both fCSs, especially fCS-Pg, could be used as a potential anti-hyperlipidemic drug.
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.
authors.forename: Nian
authors.initials: N
authors.lastname: Wu
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Ding
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China. Electronic address: chenshiguo210@163.com.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000116:Acetylgalactosamine / Q000737:chemistry; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000494:pharmacology*; D059305:Diet, High-Fat; D005643:Fucose / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000494:pharmacology; D008099:Liver / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D012616:Sea Cucumbers / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D000960:Hypolipidemic Agents; D013431:Sulfates; C517150:fucosylated chondroitin sulfate; D005643:Fucose; D013455:Sulfur; D020723:Glucuronic Acid; D002809:Chondroitin Sulfates; D000116:Acetylgalactosamine
keywords: Anti-hyperlipidemic; Fucosylated chondroitin sulfates; Isostichopus badionotus; Pearsonothuria graeffei; Sulfation pattern