title: An oncogenic role of miR-592 in tumorigenesis of human colorectal cancer by targeting Forkhead Box O3A (FoxO3A).
abstract: A microRNA (miRNA) that functionally downregulates the expression of tumor suppressors can be defined as an oncomir. Here, we interrogate the biological significance of miR-592 in colorectal cancer (CRC).
  
  
  
  The expression of miR-592 in CRC tissues and cell lines was ascertained by qRT-PCR assay, and the expression of its target gene was determined by immunohistochemistry staining. The oncogenic role of miR-592 was assessed in terms of cell proliferation, migration, and clonogenicity in vitro, whereas the tumorigenicity was assessed by inhibiting endogenous miR-592 in CRC cells in vivo.
  
  
  
  A striking upregulation of miR-592 was observed in CRC tissues and cell lines compared to the matched adjacent non-tumor tissues and normal colon cells. Importantly, Forkhead Box O3A (FoxO3A) was identified as a novel target of miR-592. miR-592 inhibitor exhibited a significant reduction of migration, proliferation, and clonogenicity in CRC cells. These cells also displayed a decreased tumorigenicity in SCID mice relative to the control cells.
  
  
  
  These data suggest that miR-592 may promote the progression and metastasis, in part, by targeting FoxO3A in CRC. miR-592 may be a novel target for CRC treatment and antagomir-592 may inhibit the proliferation and metastasis of CRC cells.
authors.affiliation: a Human Stem Cell Institute , General Hospital of Ningxia Medical University , Yinchuan , China.|b Department of Colorectal Surgery , General Hospital of Ningxia Medical University , Yinchuan , China.|c Graduate School , Ningxia Medical University , Yinchuan , China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Fu
authors.affiliation: a Human Stem Cell Institute , General Hospital of Ningxia Medical University , Yinchuan , China.|b Department of Colorectal Surgery , General Hospital of Ningxia Medical University , Yinchuan , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.affiliation: b Department of Colorectal Surgery , General Hospital of Ningxia Medical University , Yinchuan , China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: b Department of Colorectal Surgery , General Hospital of Ningxia Medical University , Yinchuan , China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: d Department of Pathology , General Hospital of Ningxia Medical University , Yinchuan , China.
authors.forename: Ningmei
authors.initials: N
authors.lastname: Zhang
authors.affiliation: a Human Stem Cell Institute , General Hospital of Ningxia Medical University , Yinchuan , China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.affiliation: e Department of Clinical Oncology , Queen Elizabeth Hospital , Kowloon , Hong Kong.
authors.forename: William C
authors.initials: WC
authors.lastname: Cho
authors.affiliation: a Human Stem Cell Institute , General Hospital of Ningxia Medical University , Yinchuan , China.|b Department of Colorectal Surgery , General Hospital of Ningxia Medical University , Yinchuan , China.
authors.forename: Yinxue
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D000071316:Forkhead Box Protein O3 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; C000588991:MIRN592 microRNA, human; D035683:MicroRNAs
keywords: Antagomir; Forkhead Box O3A; colorectal cancer; miR-592; microRNA