title: Cratylia mollis lectin nanoelectrode for differential diagnostic of prostate cancer and benign prostatic hyperplasia based on label-free detection.
abstract: The research for new biomarkers of cancer has studied the role of fetuin glycoprotein on the metastatic disease diagnosis. Cratylia mollis is a lectin with high finity to fetuin, and used here to differentiate prostate cancer and benign prostatic hyperplasia. A label-free electrochemical nanosensor based on assembled carboxylated carbon nanotubes (COOH-CNTs) and poly-L-lysine (PLL) film was developed and applied to serum samples of prostate cancer positive for Gleason score. The electrode analytical response to fetuin in PBS samples, obtained by square wave voltammetry, exhibited a linear range from 0.5 to 25µgmL(-1), with a high correlation coefficient (r=0.994, p<0.001) and low limit of detection (0.017µgmL(-1)). The lectin nanoelectrode showed a good repeatability (1.24% RSD) and reproducibility (4.24% RSD). A pool of serum samples from prostate cancer patients with known the Gleason score were tested showing a significant statistically correlation. Thus, the lectin nanoelectrode was able to distinguish the degree of staging prostate cancer, providing the diagnostic differentiation of benign and malign hyperplasia. To the best of our knowledge, it is the first biosensor for this application using a lectin.
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Recife, Brazil; Protein Biochemistry Laboratory, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Priscila M S
authors.initials: PMS
authors.lastname: Silva
authors.affiliation: Protein Biochemistry Laboratory, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Amanda L R
authors.initials: ALR
authors.lastname: Lima
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Bárbara V M
authors.initials: BVM
authors.lastname: Silva
authors.affiliation: Protein Biochemistry Laboratory, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Luana C B B
authors.initials: LCBB
authors.lastname: Coelho
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Recife, Brazil. Electronic address: rosa.dutra@ufpe.br.
authors.forename: Rosa F
authors.initials: RF
authors.lastname: Dutra
authors.affiliation: Protein Biochemistry Laboratory, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Maria T S
authors.initials: MTS
authors.lastname: Correia
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D003937:Diagnosis, Differential; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D007887:Fabaceae / Q000737:chemistry*; D060748:Fetuins / Q000032:analysis*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D057230:Limit of Detection; D008297:Male; D037742:Nanotubes, Carbon / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D011467:Prostate / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000097:blood / Q000175:diagnosis*; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D015203:Reproducibility of Results
chemical_list: D060748:Fetuins; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D037121:Plant Lectins; D011107:Polylysine
keywords: Carbon nanotube; Cratylia mollis lectin; Electrochemical biosensor; Gleason score; Poly-L-lysine; Prostate cancer