title: ABC-B transporter genes in Dirofilaria immitis.
abstract: Dirofilaria immitis is a filarial nematode causing infection and heartworm disease in dogs and other canids, cats, and occasionally in humans. Prevention with macrocyclic lactones (ML) is recommended during the mosquito transmission season. Recently, ML resistance has been reported. ABC-B transporter genes are thought to be involved in the mechanism of ML resistance in other nematodes. This study aimed to identify all the ABC-B transporter genes in D. immitis using as a reference the nDi.2.2 D. immitis whole genome, which is not completely annotated. Using bioinformatic tools and PCR amplification on pooled D. immitis genomic DNA and on pooled cDNA, nine ABC transporter genes including one pseudogene were characterized. Bioinformatic and phylogenetic analyses allowed identification of three P-glycoproteins (Pgps) (Dim-pgp-3 Dim-pgp-10, Dim-pgp-11), of two ABC-B half transporter genes (one ortholog of Cel-haf-4 and Cel-haf-9; and one ortholog of Cel-haf-1 and Cel-haf-3), of one ABC half transporter gene (ortholog of Cel-haf-5) that contained an ABC-C motif, and of one additional half transporter that would require functional study for characterization. The number of ABC-B transporter genes identified was lower than in Caenorhabditis elegans and Haemonchus contortus. Further studies are needed to understand their possible role in ML resistance in D. immitis. These ABC transporters constitute a base for ML resistance investigation in D. immitis and advance our understanding of the molecular biology of this parasite.
authors.affiliation: Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte-Anne-de-Bellevue, H9X3V9, QC, Canada.
authors.forename: Catherine
authors.initials: C
authors.lastname: Bourguinat
authors.affiliation: Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte-Anne-de-Bellevue, H9X3V9, QC, Canada.
authors.forename: Hua
authors.initials: H
authors.lastname: Che
authors.affiliation: Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte-Anne-de-Bellevue, H9X3V9, QC, Canada.
authors.forename: Thangadurai
authors.initials: T
authors.lastname: Mani
authors.affiliation: Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte-Anne-de-Bellevue, H9X3V9, QC, Canada.
authors.forename: Kathy
authors.initials: K
authors.lastname: Keller
authors.affiliation: Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte-Anne-de-Bellevue, H9X3V9, QC, Canada. Electronic address: roger.prichard@mcgill.ca.
authors.forename: Roger K
authors.initials: RK
authors.lastname: Prichard
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D017173:Caenorhabditis elegans / Q000235:genetics; D019295:Computational Biology; D004183:Dirofilaria immitis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D004351:Drug Resistance; D049751:Genome, Helminth; D006189:Haemonchus / Q000235:genetics; D007783:Lactones / Q000494:pharmacology; D047028:Macrocyclic Compounds / Q000494:pharmacology*; D016133:Polymerase Chain Reaction
chemical_list: D018528:ATP-Binding Cassette Transporters; D000871:Anthelmintics; D007783:Lactones; D047028:Macrocyclic Compounds
keywords: Dirofilaria immitis; Genetic identification; P-glycoprotein