title: Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.
abstract: The field of lymphatic research has benefited enormously from the discovery of "marker" proteins that permit not only the identification and quantitation of lymphatic vessels in tissue sections for tumor pathology but also the isolation of primary lymphatic endothelial cells for basic research. This chapter focuses on the use of these markers for the immunohistochemical analysis of lymphangiogenesis in both frozen and paraffin-embedded tissue sections and discusses current protocols including newer versions employing biotin tyramide amplification and their associated problems.
authors.affiliation: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, UK.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Royston
authors.affiliation: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, 2 AbD Serotec, Kidlington, Oxford OX5 1GE, UK.
authors.forename: Steven
authors.initials: S
authors.lastname: Clasper
authors.affiliation: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, UK. David.jackson@imm.ox.ac.uk.
authors.forename: David G
authors.initials: DG
authors.lastname: Jackson
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism; D049109:Cell Proliferation; D006801:Humans; D007150:Immunohistochemistry; D042583:Lymphangiogenesis; D042601:Lymphatic Vessels / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016612:Paraffin Embedding; D014021:Tissue Preservation; D014439:Tyramine / Q000378:metabolism
chemical_list: D015415:Biomarkers; C400422:biotin tyramine; D001710:Biotin; D014439:Tyramine
keywords: Chalkley grid; Immunofluorescence; LYVE-1; Lymphangiogenesis; Lymphatic; Peroxidase; Podoplanin