title: Mannose-binding lectin gene (MBL2) polymorphisms related to the mannose-binding lectin low levels are associated to dengue disease severity.
abstract: Dengue is the main arbovirosis in the tropical and subtropical areas of the world. The majority of infected individuals present an asymptomatic outcome while others progress to dengue fever (DF) or dengue haemorrhagic fever (DHF). Dengue infection evolution to severe outcomes is in part, related to innate immunity response. The MBL2 gene encodes for a pathogen recognition pattern molecule, the mannose-binding lectin (MBL). Variant alleles at promoter and structural regions of the MBL2 are related to serum MBL levels and function. Due to the important inflammatory modulation role of MBL, MBL2 polymorphisms could influence dengue progression. Therefore, this study investigated associations of MBL2 polymorphisms and serum MBL levels in patients with dengue. Genotyping of promoter and structural regions of MBL2 was performed by real-time PCR using Taqman® probes in 161 patients presenting DF or DHF outcome. For the serum MBL determination a commercial ELISA kit was used. The variant OO genotype and O allele were associated with DHF (p=0.008 and p=0.009 respectively). Haplotypes correlated to MBL low levels were associated with DHF (p=0.04). Our results support the hypothesis that patients carrying genotypes or haplotypes of low production of MBL would be more susceptible to DHF.
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco (UPE), Brazil.
authors.forename: Gabriela G
authors.initials: GG
authors.lastname: Figueiredo
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco (UPE), Brazil.
authors.forename: Renata D
authors.initials: RD
authors.lastname: Cezar
authors.affiliation: Faculdade de Ciências Médicas, Universidade de Pernambuco (UPE), Brazil.
authors.forename: Naishe M
authors.initials: NM
authors.lastname: Freire
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco (UPE), Brazil.
authors.forename: Vanessa G
authors.initials: VG
authors.lastname: Teixeira
authors.affiliation: Faculdade de Ciências Médicas, Universidade de Pernambuco (UPE), Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Baptista
authors.affiliation: Centro de Pesquisa Aggeu Magalhães (CPqAM/FIOCRUZ-PE), Brazil.
authors.forename: Marli
authors.initials: M
authors.lastname: Cordeiro
authors.affiliation: Colegiado de Farmácia, Universidade Federal do Vale do São Francisco (UNIVASF), Brazil.
authors.forename: Rodrigo F
authors.initials: RF
authors.lastname: Carmo
authors.affiliation: Centro de Pesquisa Aggeu Magalhães (CPqAM/FIOCRUZ-PE), Brazil; Instituto do Fígado de Pernambuco (IFP), Brazil.
authors.forename: Luydson Richardson Silva
authors.initials: LR
authors.lastname: Vasconcelos
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Pernambuco (UPE), Brazil. Electronic address: patricia.moura@upe.br.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Moura
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D019595:Severe Dengue / Q000235:genetics*; D055815:Young Adult
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin
keywords: Dengue; MBL2 gene; Mannose-Binding Lectin; Polymorphism