title: A Flow SPR Immunosensor Based on a Sandwich Direct Method.
abstract: In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10(-3) and 10(-1) M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes.
authors.affiliation: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Mauro.tomassetti@uniroma1.it.
authors.forename: Mauro
authors.initials: M
authors.lastname: Tomassetti
authors.affiliation: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. giorgia.conta@libero.it.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Conta
authors.affiliation: Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. luigi.campanella@uniroma1.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Campanella
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. gabriele.favero@uniroma1.it.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. gabriella.sanzo@uniroma1.it.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sanzò
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. franco.mazzei@uniroma1.it.
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. riccarda.antiochia@uniroma1.it.
authors.forename: Riccarda
authors.initials: R
authors.lastname: Antiochia
mesh_terms: D000667:Ampicillin; D000900:Anti-Bacterial Agents; D015374:Biosensing Techniques; D007118:Immunoassay; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin
keywords: ampicillin; flow immunosensor SPR; sandwich method