title: Differential effects of bisphenol A and estradiol on rat spermatogenesis' establishment.
abstract: Several studies have highlighted the negative effects of bisphenol A (BPA), a chemical compound with estrogenic activity, on reproductive health. To elucidate the impact of BPA on spermatogenesis' establishment and mechanisms of action of BPA and 17β-estradiol (E2), as both can be found in the environment, we exposed rats to BPA (50μg/kg bw/day of BPA), E2 (20μg/kg bw/day of E2) and BPA+E2 from 15 to 30days post-partum. Histological and gene expression studies revealed that BPA and BPA+E2 exposures promoted spermatogenesis establishment whereas E2 alone delayed it. Then, a decrease in gene expression of blood-testis-barrier (BTB) proteins was observed in all treated groups. Therefore, our study has demonstrated a differential effect of BPA and E2 exposures on spermatogenesis establishment in prepubertal rats and a deleterious effect of these chemicals on BTB establishment. Thus, the effects of BPA seem to be mediated by receptors other than estrogen receptors.
authors.affiliation: Normandie Univ., France; UNICAEN, EA2608, F-14032 Caen, France; INRA USC 1377, F-14032 Caen, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Brouard
authors.affiliation: Normandie Univ., France; UNICAEN, EA2608, F-14032 Caen, France; INRA USC 1377, F-14032 Caen, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Guénon
authors.affiliation: Normandie Univ., France; UNICAEN, EA2608, F-14032 Caen, France; INRA USC 1377, F-14032 Caen, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Bouraima-Lelong
authors.affiliation: Normandie Univ., France; UNICAEN, EA2608, F-14032 Caen, France; INRA USC 1377, F-14032 Caen, France. Electronic address: christelle.delalande@unicaen.fr.
authors.forename: Christelle
authors.initials: C
authors.lastname: Delalande
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000097:blood / Q000633:toxicity*; D004967:Estrogens / Q000097:blood / Q000633:toxicity*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004958:Estradiol; D004967:Estrogens; D010636:Phenols; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A (BPA); Blood testis barrier (BTB); Estradiol (E2); Spermatogenesis