title: Bovine serum albumin interacts with silver nanoparticles with a "side-on" or "end on" conformation.
abstract: As the nanoparticles (NPs) enter into the biological interface, they have to encounter immediate and first exposure to many proteins of different concentrations. The physicochemical interaction of NPs and proteins is greatly influenced not only by the number and type of proteins; but also the surface chemistry of NPs. To analyze the effects of NPs on proteins, the interaction between bovine serum albumin (BSA) and silver nanoparticles (AgNPs) at different concentrations were investigated. The interaction, BSA conformations, kinetics and adsorption were analyzed by UV-Visible spectrophotometer, dynamic light scattering (DLS), FT-IR spectroscopy and fluorescence quenching. DLS, FTIR and UV-visible spectrophotometric analysis confirms the interaction with minor alterations in size of the protein. Fluorescence quenching analysis confirms the side-on or end-on interaction of 1.5 molecules of BSA to AgNP. Further, pseudo-second order kinetics was determined with equilibrium contact-time of 30 min. The data of the present study determines the detailed evaluation of BSA adsorption on AgNP along with mechanism, kinetics and isotherm of the adsorption.
authors.affiliation: Nano-food Research Group, Instrumental and Food Analysis Laboratory, Industrial Biotechnology Division, School of BioSciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors.forename: Nandita
authors.initials: N
authors.lastname: Dasgupta
authors.affiliation: Nano-food Research Group, Instrumental and Food Analysis Laboratory, Industrial Biotechnology Division, School of BioSciences and Technology, VIT University, Vellore, Tamil Nadu, India; Research Wing, Veer Kunwar Singh Memorial Trust, Chapra, Bihar, India; Xpert Arena Technological Services Pvt. Ltd., Chapra, India. Electronic address: shivenduranjan@gmail.com.
authors.forename: Shivendu
authors.initials: S
authors.lastname: Ranjan
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
authors.forename: Dhabaleswar
authors.initials: D
authors.lastname: Patra
authors.affiliation: Division of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Srivastava
authors.affiliation: Institute of Life Sciences, School of Science and Technology, Ahmedabad University, Ahmedabad, Gujarat, India.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Nano-food Research Group, Instrumental and Food Analysis Laboratory, Industrial Biotechnology Division, School of BioSciences and Technology, VIT University, Vellore, Tamil Nadu, India. Electronic address: cramlingam@vit.ac.in.
authors.forename: Chidambaram
authors.initials: C
authors.lastname: Ramalingam
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D000067493:Dynamic Light Scattering; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D012710:Serum Albumin, Bovine; D012834:Silver
keywords: Bovine serum albumin; Langmuir; Nanoparticle; Order of reaction; Protein interaction; Silver