title: Placental microRNA expression in pregnancies complicated by superimposed pre‑eclampsia on chronic hypertension.
abstract: Pre-eclampsia (PE) is a complication of pregnancy that affects 5‑8% of women after 20 weeks of gestation. It is usually diagnosed based on the de novo onset of hypertension and proteinuria. Preexisting hypertension in women developing PE, also known as superimposed PE on chronic hypertension (SPE), leads to elevated risk of maternal and fetal mortality. PE is associated with an altered microRNA (miRNA) expression pattern in the placenta, suggesting that miRNA deregulation is involved in the pathogenesis of PE. Whether and how the miRNA expression pattern is changed in the SPE placenta remains unclear. The present study analyzed the placental miRNA expression profile in pregnancies complicated by SPE. miRNA expression profiles in SPE and normal placentas were investigated using an Ion Torrent sequencing system. Sequencing data were processed using a comprehensive analysis pipeline for deep miRNA sequencing (CAP‑miRSeq). A total of 22 miRNAs were identified to be deregulated in placentas from patients with SPE. They included 16 miRNAs previously known to be associated with PE and 6 novel miRNAs. Among the 6 novel miRNAs, 4 were upregulated (miR‑518a, miR‑527, miR‑518e and miR‑4532) and 2 downregulated (miR‑98 and miR‑135b) in SPE placentas compared with controls. The present results suggest that SPE is associated with specific alterations in the placental miRNA expression pattern, which differ from alterations detected in PE placentas, and therefore, provide novel targets for further investigation of the molecular mechanisms underlying SPE pathogenesis.
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Elena S
authors.initials: ES
authors.lastname: Vashukova
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Glotov
authors.affiliation: Computer Technologies Laboratory, ITMO University, St. Petersburg 197101, Russian Federation.
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Fedotov
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Efimova
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Pakin
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Mozgovaya
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Pendina
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Tikhonov
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Alla S
authors.initials: AS
authors.lastname: Koltsova
authors.affiliation: D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, St. Petersburg 199034, Russian Federation.
authors.forename: Vladislav S
authors.initials: VS
authors.lastname: Baranov
mesh_terms: D000328:Adult; D001794:Blood Pressure; D016022:Case-Control Studies; D019295:Computational Biology / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D006973:Hypertension / Q000150:complications*; D035683:MicroRNAs / Q000235:genetics*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000209:etiology* / Q000503:physiopathology*; D011247:Pregnancy
chemical_list: D035683:MicroRNAs