title: Screening and chromosome localization of two cotton BAC clones.
abstract: Two bacterial artificial chromosome (BAC) clones (350B21 and 299N22) of Pima 90-53 cotton [Gossypium barbadense Linnaeus, 1753 (2n=4x=52)] were screened from a BAC library using SSR markers. Strong hybridization signals were detected at terminal regions of all A genome (sub-genome) chromosomes, but were almost absent in D genome (sub-genome) chromosomes with BAC clone 350B21 as the probe. The results indicate that specific sequences, which only exist at the terminal parts of A genome (sub-genome) chromosomes with a huge repeat number, may be contained in BAC clone 350B21. When utilizing FISH with the BAC clone 299N22 as probe, a pair of obvious signals was detected on chromosome 13 of D genome (sub-genome), while strong dispersed signals were detected on all A genome (sub-genome) chromosomes. The results showed that peculiar repetitive sequence, which was distributed throughout all A genome (sub-genome) chromosomes, may exist in BAC clone 299N22. The absence of the repetitive sequences, which exist in the two BAC clones, in D genome may account for the genome-size variation between A and D genomes. In addition, the microcolinearity analysis of the clone 299N22 and its homologous region on Gossypium raimondii Ulbrich, 1932 chromosome 13 (D513) indicated that the clone 299N22 might come from A sub-genome of sea island cotton (Gossypium barbadense), and a huge number of small deletions, illegitimate recombination, translocation and rearrangements may have occurred during the genus evolution. The two BAC clones studied here can be used as cytological markers but will be also be helpful to research in cotton genome evolution and comparative genomics.
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Xinglei
authors.initials: X
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.lastname: Yanyan Zhao
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Fei
authors.initials: F
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China; Anyang Institute of Technology, Anyang, Henan, 455000, China.
authors.forename: Renhai
authors.initials: R
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000,China.
authors.forename: Kunbo
authors.initials: K
authors.lastname: Wang
keywords: BAC; Cotton; FISH; cytological marker; microcolinearity