title: Diastolic dysfunction and cardiac troponin I decrease in aging hearts.
abstract: Cardiac tropnoin I (cTnI) plays a critical role in the regulation of diastolic function, and its low expression may result in cardiac diastolic dysfunction, which is the most common form of cardiovascular disorders in older adults. In this study, cTnI expression levels were determined in mice at various ages and cardiac function was measured and compared between young adult mice (3 and 10 months) and older mice (18 months). The data indicated that the cTnI levels reached a peak high in young adult hearts (3 months), but decreased in older hearts (18 months). Furthermore, the older hearts showed a significant diastolic dysfunction observed by P-V loop and echocardiography measurements. To further define the mechanism underlying the cTnI decrease in aging hearts, we tested DNA methylation and histone acetylation modifications of cTnI gene. We found that acetylation of histone near the promoter region of cTnI gene played an important role in regulation of cTnI expression in the heart at different ages. Our study indicates that epigenetic modification caused cTnI expression decrease is one of the possible causes that result in a reduced cTnI level and diastolic dysfunction in the older hearts.
authors.affiliation: Heart Centre, Children's Hospital of Chongqing Medical University, Chongqing, PR China; Key Laboratory of Developmental Disease in Childhood (Chongqing Medical University), Ministry of Education, Chongqing, PR China; Key Laboratory of Pediatrics in Chongqing, PR China; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, PR China.
authors.forename: B
authors.initials: B
authors.lastname: Pan
authors.affiliation: Heart Centre, Children's Hospital of Chongqing Medical University, Chongqing, PR China; Key Laboratory of Developmental Disease in Childhood (Chongqing Medical University), Ministry of Education, Chongqing, PR China; Key Laboratory of Pediatrics in Chongqing, PR China; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, PR China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Xu
authors.affiliation: Heart Centre, Children's Hospital of Chongqing Medical University, Chongqing, PR China; Key Laboratory of Developmental Disease in Childhood (Chongqing Medical University), Ministry of Education, Chongqing, PR China; Key Laboratory of Pediatrics in Chongqing, PR China; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Developmental Disease in Childhood (Chongqing Medical University), Ministry of Education, Chongqing, PR China; Key Laboratory of Pediatrics in Chongqing, PR China; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, PR China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Developmental Disease in Childhood (Chongqing Medical University), Ministry of Education, Chongqing, PR China; Key Laboratory of Pediatrics in Chongqing, PR China; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Biomedical Science, Charlie E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biomedical Science, Charlie E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA.
authors.forename: C
authors.initials: C
authors.lastname: Nan
authors.affiliation: Department of Biomedical Science, Charlie E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Science, Charlie E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA.
authors.forename: W
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Biomedical Science, Charlie E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA. Electronic address: xhuang@health.fau.edu.
authors.forename: X P
authors.initials: XP
authors.lastname: Huang
authors.affiliation: Heart Centre, Children's Hospital of Chongqing Medical University, Chongqing, PR China. Electronic address: jietian@cqmu.edu.cn.
authors.forename: J
authors.initials: J
authors.lastname: Tian
mesh_terms: D000375:Aging; D000818:Animals; D009202:Cardiomyopathies / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D003971:Diastole; D004452:Echocardiography; D044127:Epigenesis, Genetic; D006321:Heart / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D019210:Troponin I / Q000502:physiology*
chemical_list: D019210:Troponin I
keywords: Diastolic dysfunction; Epigenetics; Gene expression; Histone acetylation; Troponin