title: Expression and function of microRNA-497 in human osteosarcoma.
abstract: The expression and function of microRNA-497 (miR-497) has previously been reported in various types of human cancer; however, miR-497 has not previously been investigated in human osteosarcoma (OS). In the present study, the expression levels of miR‑497 were analyzed by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in OS tissues and cell lines. In addition, post-transfection with miR‑497, RT‑qPCR, cell proliferation, migration and invasion assays, western blot analysis, and luciferase assays were performed in OS cell lines. The results of the present study demonstrated that miR‑497 was downregulated in OS tissues and cells compared with normal controls. Furthermore, upregulation of miR‑497 inhibited cell proliferation, migration and invasion in osteosarcoma cell lines compared with the negative control group. In addition, the present study demonstrated that miR‑497 may function by directly targeting insulin‑like growth factor 1 receptor in OS cells. These findings indicated that miR‑497 may be useful as a therapeutic target for the treatment of OS.
authors.affiliation: Department of Orthopaedic Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Orthopaedic Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Ankit
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Orthopaedic Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Fenyong
authors.initials: F
authors.lastname: Shou
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010006:Osteoblasts / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics*; D034622:RNA Interference; D017526:Receptor, IGF Type 1 / Q000235:genetics
chemical_list: C559640:MIRN497 microRNA, human; D035683:MicroRNAs; D017526:Receptor, IGF Type 1