title: miR-937 contributes to the lung cancer cell proliferation by targeting INPP4B.
abstract: Lung cancer is the leading cause of cancer death worldwide, microRNAs play critical role in the initiation and development of lung cancer. Here, we used MTT assay, colony formation assay, soft agar growth assay and BrdU incorporation assay to investigate miR-937's role in lung cancer. We found that miR-937 was upregulated in lung cancer tissues and cells. Overexpression of miR-937 in A549 promoted anchorage -dependent and -independent growth, whereas knockdown of miR-937 reduced this effect. Meanwhile, we also found miR-937 overexpression increased CCND1 and c-Myc levels in both mRNA and protein levels, knockdown of miR-937 reduced this effect, confirming miR-937 promoted cell proliferation. Mechanism analyses found polyphosphate 4-phosphatase type II (INPP4B) was the target of miR-937, miR-937 directly bound to the 3'UTR of INPP4B, knockdown of INPP4B in A549 with miR-937 inhibitor promoted anchorage -dependent and -independent growth, suggesting miR-937 contributed to cell proliferation of lung cancer by inhibiting INPP4B, it might be a valuable target for lung cancer therapy.
authors.affiliation: Department of Geriatric Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Daxiong
authors.initials: D
authors.lastname: Zeng
authors.affiliation: Department of Respiratory Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
authors.forename: Yantian
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Respiratory Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China. Electronic address: xuxiao81179@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Respiratory Medicine, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China. Electronic address: xuguopeng2046@foxmail.com.
authors.forename: Guopeng
authors.initials: G
authors.lastname: Xu
mesh_terms: D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C000614456:MIRN937 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D010744:Phosphoric Monoester Hydrolases; C053153:phosphatidylinositol-3,4-bisphosphate 4-phosphatase
keywords: Cell proliferation; INPP4B; Lung cancer; miR-935