title: Evaluation of genetic diversity amongst Descurainia sophia L. genotypes by inter-simple sequence repeat (ISSR) marker.
abstract: Descurainia sophia is a valuable medicinal plant in family of Brassicaceae. To determine the range of diversity amongst D. sophia in Iran, 32 naturally distributed plants belonging to six natural populations of the Iranian plateau were investigated by inter-simple sequence repeat (ISSR) markers. The average percentage of polymorphism produced by 12 ISSR primers was 86 %. The PIC values for primers ranged from 0.22 to 0.40 and Rp values ranged between 6.5 and 19.9. The relative genetic diversity of the populations was not high (Gst =0.32). However, the value of gene flow revealed by the ISSR marker was high (Nm = 1.03). UPGMA clustering method based on Jaccard similarity coefficient grouped the genotypes into two major clusters. Graph results from Neighbor-Net Network generated after a 1000 bootstrap test using Jaccard coefficient, and STRUCTURE analysis confirmed the UPGMA clustering. The first three PCAs represented 57.31 % of the total variation. The high levels of genetic diversity were observed within populations, which is useful in breeding and conservation programs. ISSR is found to be an eligible marker to study genetic diversity of D. sophia.
authors.affiliation: Faculty of Agriculture, Department of Biotechnology, Bu Ali-Sina University, Hamedan, 6517838695 Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Saki
authors.affiliation: Faculty of Agriculture, Department of Biotechnology, Bu Ali-Sina University, Hamedan, 6517838695 Iran.
authors.forename: Hedayat
authors.initials: H
authors.lastname: Bagheri
authors.affiliation: Faculty of Agriculture, Department of Biotechnology, Bu Ali-Sina University, Hamedan, 6517838695 Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Deljou
authors.affiliation: Agriculture Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, Mahdasht Road, Karaj, 315351897 Iran.
authors.forename: Mehrshad
authors.initials: M
authors.lastname: Zeinalabedini
keywords: Descurainia sophia; Genetic diversity; ISSR marker; Iranian plateau