title: Activation of focal adhesion kinase through an interaction with β4 integrin contributes to tumorigenicity of colon cancer.
abstract: High expression of either β4 integrin or focal adhesion kinase (FAK) has been reported in human colon cancer. However, it remains unclear how β4 integrin together with FAK contributes to the tumorigenicity of colon cancer. Here, we demonstrate that the co-overexpression of β4 integrin and FAK positively correlates with advanced stages of human colon cancer. Activated β4 integrin interacts with FAK and subsequently induces FAK phosphorylation at Tyr397. Furthermore, ablation of the β4 integrin/FAK complex and/or FAK activation impair colon cancer cell proliferation, anchorage-independent growth, and tumorigenicity. Our data indicate that the β4 integrin/FAK complex and subsequent FAK activation are essential regulators during the tumorigenicity of colon cancer, and we suggest an alternative strategy for colon cancer therapy.
authors.affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Tai
authors.affiliation: Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.|Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: I-Rue
authors.initials: IR
authors.lastname: Lai
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Peng
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.|Center for Biotechnology, National Taiwan University, Taipei, Taiwan.
authors.forename: Shih-Torng
authors.initials: ST
authors.lastname: Ding
authors.affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.|Center for Biotechnology, National Taiwan University, Taipei, Taiwan.
authors.forename: Tang-Long
authors.initials: TL
authors.lastname: Shen
mesh_terms: D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D039663:Integrin beta4 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000235:genetics
chemical_list: C059834:ITGB4 protein, human; D039663:Integrin beta4; D009363:Neoplasm Proteins; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human
keywords: FAK; human colon cancer; β4 integrin