title: Generation of Rat Monoclonal Antibodies Against Human Pancreatic Ductal Adenocarcinoma Cells.
abstract: Pancreatic ductal adenocarcinoma is an aggressive tumor with a poor prognosis. Biomarkers that can detect the tumor in its early stages when it may be amenable to curative resection might improve prognosis. To discover novel markers expressed in primary pancreatic cancer, we generated a panel of monoclonal antibodies against pancreatic ductal adenocarcinoma cell line BxPC3 using a rat medial iliac lymph node method. The antigen recognized by 1B5A5 was expressed on the cell surface and secreted into the conditioned medium of BxPC3 cells, and characterized as glycoproteins with molecular mass between 60 and 90 kDa. A wide range of molecular weights of 1B5A5 antigen in several pancreatic cancer cell lines were observed. Immunohistochemistry using a human multiple organ tumor tissue array showed an enhanced expression of 1B5A5 antigen in pancreas, lung, stomach, breast, urinary bladder, colon, and cervix uteri cancers. Immunoprecipitation followed by proteomic analyses identified CEACAM6 as a 1B5A5 antigen. In addition, western blot analysis results indicated that the 1B5A5 epitope is located within an amino-terminal domain of CEACAM6. These results raised the possibility that our approach could lead to discovery of novel biomarkers for the early stage of cancers in a relatively short period of time.
authors.affiliation: 1 Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd. , Osaka, Japan .
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Higashi
authors.affiliation: 2 Department of Bioengineering, Graduate School of Engineering, Osaka City University , Osaka, Japan .
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Fujii
authors.affiliation: 1 Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd. , Osaka, Japan .
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kushida
authors.affiliation: 3 Laboratory of Toxicology, Faculty of Pharmacy, Osaka Ohtani University , Osaka, Japan .
authors.forename: Keita
authors.initials: K
authors.lastname: Yamada
authors.affiliation: 1 Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd. , Osaka, Japan .
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: 1 Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd. , Osaka, Japan .
authors.forename: Koichi
authors.initials: K
authors.lastname: Saito
authors.affiliation: 2 Department of Bioengineering, Graduate School of Engineering, Osaka City University , Osaka, Japan .
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000276:immunology; D014408:Biomarkers, Tumor / Q000276:immunology*; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D058851:GPI-Linked Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D011379:Prognosis; D051381:Rats; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C504117:CEACAM6 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins