title: Cell interaction with modified nanotubes formed on titanium alloy Ti-6Al-4V.
abstract: Nanotubes with diameters ranging from 40 to 60nm were prepared by electrochemical oxidation of the Ti-6Al-4V alloy in electrolyte containing ammonium sulphate and ammonium fluoride. The nanotubes were further modified with calcium and phosphate ions or were heat treated. Polished Ti-6Al-4V alloy served as a reference sample. The spreading of human osteoblast-like cells was similar on all nanotube samples but lower than on polished samples. The number of initially adhered cells was higher on non-modified nanotubes, but the final cell number was the highest on Ca-enriched nanotubes and the lowest on heat-treated nanotubes. However, these differences were relatively small and less pronounced than the differences in the concentration of specific molecular markers of cell adhesion and differentiation, estimated by their intensity of immunofluorescence staining. The concentration of vinculin, i.e. a protein of focal adhesion plaques, was the lowest on nanotubes modified with calcium. Collagen I, an early marker of osteogenic cell differentiation, was also the lowest on samples modified with calcium and was highest on polished samples. Alkaline phosphatase, a middle marker of osteogenic differentiation, was observed in lowest concentration on nanotubes modified with phosphorus and the highest on heat-treated samples. Osteocalcin concentrations, a late marker of osteogenic cell differentiation, were similar on all tested samples, although they tended to be the highest on heat-treated samples. Thus, osteogenic differentiation can be modulated by various additional treatments of nanotube coatings on Ti-6Al-4V implants.
authors.affiliation: University of Chemistry and Technology, Prague, Department of Metals and Corrosion Engineering, Technicka 5, Prague 6, Czech Republic. Electronic address: moravech@vscht.cz.
authors.forename: Hynek
authors.initials: H
authors.lastname: Moravec
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Vandrovcova
authors.affiliation: University of Chemistry and Technology, Prague, Department of Metals and Corrosion Engineering, Technicka 5, Prague 6, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Chotova
authors.affiliation: University of Chemistry and Technology, Prague, Department of Metals and Corrosion Engineering, Technicka 5, Prague 6, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Fojt
authors.affiliation: University of Chemistry and Technology, Prague, Department of Metals and Corrosion Engineering, Technicka 5, Prague 6, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Pruchova
authors.affiliation: University of Chemistry and Technology, Prague, Department of Metals and Corrosion Engineering, Technicka 5, Prague 6, Czech Republic.
authors.forename: Ludek
authors.initials: L
authors.lastname: Joska
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
mesh_terms: D000497:Alloys; D002118:Calcium / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology; D016596:Vinculin / Q000737:chemistry
chemical_list: D000497:Alloys; D024042:Collagen Type I; D015675:Osteocalcin; D016596:Vinculin; C031462:titanium alloy (TiAl6V4); D014025:Titanium; D002118:Calcium
keywords: Cell interaction; Electrochemical oxidation; Hydrothermal modification; Protein adsorption; Thermal treatment; Titanium