title: A Rapid and Convenient Method for in Vivo Fluorescent Imaging of Protoscolices of Echinococcus multilocularis.
abstract: Human and animal alveolar echinococcosis (AE) are important helminth infections endemic in wide areas of the Northern hemisphere. Monitoring Echinococcus multilocularis viability and spread using real-time fluorescent imaging in vivo provides a fast method to evaluate the load of parasite. Here, we generated a kind of fluorescent protoscolices in vivo imaging model and utilized this model to assess the activity against E. multilocularis protoscolices of metformin (Met). Results indicated that JC-1 tagged E. multilocularis can be reliably and confidently used to monitor protoscolices in vitro and in vivo. The availability of this transient in vivo fluorescent imaging of E. multilocularis protoscolices constitutes an important step toward the long term bio-imaging research of the AE-infected mouse models. In addition, this will be of great interest for further research on infection strategies and development of drugs and vaccines against E. multilocularis and other cestodes.
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Sibo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Xuyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Jixue
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.|Laboratory of Transitional Medicine, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Wu
mesh_terms: D015766:Albendazole / Q000494:pharmacology*; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles; D002232:Carbocyanines; D004195:Disease Models, Animal; D004443:Echinococcosis; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000000981:diagnostic imaging* / Q000469:parasitology; D048210:Echinococcus multilocularis / Q000187:drug effects*; D005849:Gerbillinae / Q000469:parasitology; D006801:Humans; D008297:Male; D008687:Metformin / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods*; D059208:Parasite Load / Q000379:methods
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D002232:Carbocyanines; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; D008687:Metformin; D015766:Albendazole
keywords: Echinococcus multilocularis; animal model; fluorescent imaging; in vivo