title: Transition of Immunohistochemical Expression of E-Cadherin and Vimentin from Premalignant to Malignant Lesions of Oral Cavity and Oropharynx.
abstract: OBJECTIVE
  We sought to study the expression of epithelial-to-mesenchymal transition markers E-cadherin and vimentin in precancerous lesions of the oral cavity and oropharynx and to use the specific pattern of expression to predict invasiveness.
  
  
  METHODS
  This cross-sectional study looked at 87 cases of oral and oropharyngeal lesions obtained between December 2012 and November 2014 in the Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, India. Fifty-three biopsies from the buccal mucosa, tongue, and pharynx and 34 resected oral specimens were evaluated for premalignant and malignant lesions using hematoxylin and eosin and immunohistochemical stains. Immunohistochemical expression of epithelial marker E-cadherin and mesenchymal marker vimentin was evaluated wherever possible. Slides were examined for staining pattern (cytoplasmic or membrane), proportion, and intensity of staining of tumor cells. Patients follow-up and therapy related changes were also studied.
  
  
  RESULTS
  There were 64 premalignant and 23 malignant cases in our study with 65 (74.7%) cases seen in males and 22 (25.3%) cases seen in females. The majority of malignant cases, (n = 15; 64.2%) were seen in the fifth and sixth decades of life while most of the premalignant lesions (n = 36; 56.4%) were seen in the fourth and fifth decade. Amongst the 64 premalignant oral lesions, leukoplakia comprised of 14 cases (21.9%), of which three cases had associated mild to moderate dysplasia. The majority of premalignant lesions showed strong E-cadherin expression and decreased expression of vimentin with negative and weak expression in both dysplasias and carcinoma in situ (p = 0.013). E-cadherin expression was significantly reduced in invasive carcinomas compared to dysplasias and carcinoma in situ and the difference in immunoreactivity was statistically significant (p < 0.050). Vimentin expression increased as the tumor progressed from dysplasias to carcinoma in situ to invasive carcinomas (p < 0.050).
  
  
  CONCLUSIONS
  Invasiveness of cancer can be analyzed using E-cadherin and vimentin immunohistochemical stains, which can help in predicting tumor behavior. These biomolecules can also be used as biomarkers for further research on the microinvasion of oral cancers for early diagnosis.
authors.affiliation: Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Kafil
authors.initials: K
authors.lastname: Akhtar
authors.affiliation: Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Anjum
authors.initials: A
authors.lastname: Ara
authors.affiliation: Department of Radiotherapy, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Shahid A
authors.initials: SA
authors.lastname: Siddiqui
authors.affiliation: Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Rana K
authors.initials: RK
authors.lastname: Sherwani
keywords: E-cadherin; Immunohistochemistry; Oral Neoplasms; Precancerous Conditions; Vimentin