title: Triplex-forming peptide nucleic acid modified with 2-aminopyridine as a new tool for detection of A-to-I editing.
abstract: RNA editing from adenosine to inosine (A-to-I editing) is one of the mechanisms that regulate and diversify the transcriptome. Here, a triplex-forming peptide nucleic acid (PNA) modified with a 2-aminopyridine nucleobase was applied for the recognition of the A-to-I editing event in double-stranded RNAs. The triplex-forming PNA enabled sequence-specific detection of single nucleobase editing at sub-nanomolar concentration.
authors.affiliation: Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan. sugimoto@konan-u.ac.jp.
authors.forename: Chiara
authors.initials: C
authors.lastname: Annoni
authors.forename: Tamaki
authors.initials: T
authors.lastname: Endoh
authors.forename: Dziyana
authors.initials: D
authors.lastname: Hnedzko
authors.forename: Eriks
authors.initials: E
authors.lastname: Rozners
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D000241:Adenosine / Q000032:analysis*; D000631:Aminopyridines / Q000737:chemistry*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007288:Inosine / Q000032:analysis*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000631:Aminopyridines; D020135:Peptide Nucleic Acids; D007288:Inosine; D000241:Adenosine; C032439:alpha-aminopyridine