title: Isolation and characterization of microsatellite markers in Atrina vexillum Born.
abstract: Atrina vexillum Born is an economically valuable species, widely distributed in the coastal waters of temperate and tropical areas of the Asia Pacific region. Twenty one novel microsatellite loci were identified in the genome of A. vexillum Born using the protocol for fast isolation by amplified fragment length polymorphism of sequence containing repeats. Thirty-two wild type individuals were used to evaluate the degree of polymorphism of these markers. We identified 13 polymorphic and 8 monomorphic loci with the number of alleles per locus and the polymorphism information content ranging from 2 to 5 and 0.141 to 0.664, respectively. The observed and expected heterozygosity varied from 0.1250 to 0.7000 and 0.1223 to 0.6216, respectively. Two loci deviated significantly from Hardy-Weinberg equilibrium (HWE) after Bonferroni correction, whereas the other loci were in HWE. These loci are expected to provide useful information for population genetic studies of A. vexillum Born.
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Ning
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Li
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Huang
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Mao
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
authors.forename: B B
authors.initials: BB
authors.lastname: Li
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen, China.
authors.forename: J B
authors.initials: JB
authors.lastname: Shangguan
mesh_terms: D000483:Alleles; D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D005819:Genetic Markers; D016678:Genome; D006579:Heterozygote; D018895:Microsatellite Repeats; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D005819:Genetic Markers