title: Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus regius).
abstract: The meagre (Argyrosomus regius) is taking on increasing importance in the aquaculture industry. In view of the limited supply of fish oil (FO) as a feed ingredient, the study of the capacity to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from alternative dietary oil sources is important. We analyzed changes in fatty acid (FA) desaturase 2 (fads2) and FA elongase 5 (elovl5) mRNA levels in livers and brains in response to FO replacement with a blend of vegetable oils (VO) and selenium (Se) supplementation. Fish were fed for 60 days with either a diet containing FO or a diet including VO, each supplemented or not with organic Se. Results showed that fads2 and elovl5 transcription was higher in liver when fish were fed VO diets. The brain mRNA levels of both genes were not affected by the dietary replacement of FO by VO. FA composition in the liver and skeletal muscle was altered by FO replacement, particularly by decreasing eicosapentaenoic acid and docosahexaenoic acid contents. The α-linolenic, linoleic, and arachidonic acid contents increased in both liver and brain of fish fed VO diets. The effect of Se supplementation on lipid metabolism was evident only in fish fed FO, showing a decrease in the transcription of hepatic fads2. Results indicate that the total replacement of FO by VO in diets modulates the expression of genes involved in LC-PUFA biosynthesis in meagre, affecting the FA profile of the fish flesh.
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.|ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Francisca
authors.initials: F
authors.lastname: Silva-Brito
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal. lmagnoni@ciimar.up.pt.|IIB-INTECH - Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (CONICET), Chascomús, Argentina. lmagnoni@ciimar.up.pt.
authors.forename: Leonardo J
authors.initials: LJ
authors.lastname: Magnoni
authors.affiliation: UFPB - Universidade Federal da Paraíba, Paraíba, Brazil.|CCTA - Centro de Ciencias e Tecnologia Agroalimentar, UFCG - Universidade Federal de Campina Grande, Paraíba, Brazil.
authors.forename: Sthelio Braga
authors.initials: SB
authors.lastname: Fonseca
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.|ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Peixoto
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.|FCUP - Faculty of Sciences, University of Porto, Porto, Portugal.
authors.forename: L Filipe C
authors.initials: LF
authors.lastname: Castro
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Cunha
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.|ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Rodrigo Otávio
authors.initials: RO
authors.lastname: de Almeida Ozório
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.|ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Fernando Antunes
authors.initials: FA
authors.lastname: Magalhães
authors.affiliation: CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, University of Porto, Rua dos Bragas n.289, 4050-123, Porto, Portugal.|ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: José Fernando Magalhães
authors.initials: JF
authors.lastname: Gonçalves
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage; D019587:Dietary Supplements; D044943:Fatty Acid Desaturases / Q000235:genetics*; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D010473:Perciformes / Q000235:genetics*; D010938:Plant Oils / Q000008:administration & dosage* / Q000494:pharmacology; D012643:Selenium / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D029941:Fish Proteins; D010938:Plant Oils; D044943:Fatty Acid Desaturases; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases; D012643:Selenium
keywords: Fatty acid desaturases; Fatty acid elongase; Fish oil replacement; Meagre