title: Expression of Vitis amurensis NAC26 in Arabidopsis enhances drought tolerance by modulating jasmonic acid synthesis.
abstract: The growth and fruit quality of grapevines are widely affected by abnormal climatic conditions such as water deficits, but many of the precise mechanisms by which grapevines respond to drought stress are still largely unknown. Here, we report that VaNAC26, a member of the NAC transcription factor family, was upregulated dramatically during cold, drought and salinity treatments in Vitis amurensis, a cold and drought-hardy wild Vitis species. Heterologous overexpression of VaNAC26 enhanced drought and salt tolerance in transgenic Arabidopsis. Higher activities of antioxidant enzymes and lower concentrations of H2O2 and O2 (-) were found in VaNAC26-OE lines than in wild type plants under drought stress. These results indicated that scavenging by reactive oxygen species (ROS) was enhanced by VaNAC26 in transgenic lines. Microarray-based transcriptome analysis revealed that genes related to jasmonic acid (JA) synthesis and signaling were upregulated in VaNAC26-OE lines under both normal and drought conditions. VaNAC26 showed a specific binding ability on the NAC recognition sequence (NACRS) motif, which broadly exists in the promoter regions of upregulated genes in transgenic lines. Endogenous JA content significantly increased in the VaNAC26-OE lines 2 and 3. Our data suggest that VaNAC26 responds to abiotic stresses and may enhance drought tolerance by transcriptional regulation of JA synthesis in Arabidopsis.
authors.affiliation: Beijing Key Laboratory of Grape Sciences and Enology, Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Linchuan
authors.initials: L
authors.lastname: Fang
authors.affiliation: Beijing Key Laboratory of Grape Sciences and Enology, Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Lingye
authors.initials: L
authors.lastname: Su
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Cell and Development Biology, College of Life Science, State Key Laboratory of Plant Hybrid Rice, Wuhan University, Wuhan 430072, China.
authors.forename: Xinbo
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cell and Development Biology, College of Life Science, State Key Laboratory of Plant Hybrid Rice, Wuhan University, Wuhan 430072, China.
authors.forename: Mengxiang
authors.initials: M
authors.lastname: Sun
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Sospeter Karanja
authors.initials: SK
authors.lastname: Karungo
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Fang
authors.affiliation: National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Chu
authors.affiliation: Beijing Key Laboratory of Grape Sciences and Enology, Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China shhli@ibcas.ac.cn xinhaiping215@hotmail.com.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China shhli@ibcas.ac.cn xinhaiping215@hotmail.com.
authors.forename: Haiping
authors.initials: H
authors.lastname: Xin
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology; D003517:Cyclopentanes / Q000378:metabolism*; D003681:Dehydration; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D017343:Genes, Plant / Q000502:physiology; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000502:physiology; D030821:Plants, Genetically Modified; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D027843:Vitis / Q000378:metabolism* / Q000502:physiology
chemical_list: D000975:Antioxidants; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D014157:Transcription Factors; C011006:jasmonic acid
keywords: Drought stress; JA biosynthesis; ROS; VaNAC26; Vitis amurensis.; transcriptional regulation