title: The lipopeptide 6-2 produced by Bacillus amyloliquefaciens anti-CA has potent activity against the biofilm-forming organisms.
abstract: Both the whole cells and protoplasts of Pseudomonas aeruginosa PAO1 and Bacillus cereus, two biofilm-forming bacteria, were disrupted by the lipopeptide 6-2 produced by Bacillus amyloliquefaciens anti-CA. The lipopeptide 6-2 could also effectively inhibit the formation of biofilms and disperse pre-formed biofilms. Live/dead staining of the biofilms grown in the absence or presence of the lipopeptide 6-2 showed that more dead bacterial cells in the presence of the lipopeptide than those in the absence of the lipopeptide and biofilm formation was greatly reduced by the lipopeptide 6-2. Expression of the PslC gene related to exopolysaccharides in P. aeruginosa PAO1 was also inhibited. All these results demonstrated that the lipopeptide 6-2 produced by B. amyloliquefaciens anti-CA had a high activity against biofilm-forming bacteria. The lipopeptide 6-2 also killed the larvae of Balanus amphitrite and inhibit the germination of Laminaria japonica spore and growth of protozoa, all of which were the fouling organisms in marine environments.
authors.affiliation: School of Pharmacy, Weifang Medical University, Weifang 261042, China. Electronic address: songsbob@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.affiliation: School of Pharmacy, Weifang Medical University, Weifang 261042, China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: Wang
authors.affiliation: Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
authors.forename: Guang-Lei
authors.initials: GL
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Weifang Medical University, Weifang 261042, China.
authors.forename: Wan-Zhong
authors.initials: WZ
authors.lastname: Li
authors.affiliation: School of Pharmacy, Weifang Medical University, Weifang 261042, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yan
mesh_terms: D000069977:Bacillus amyloliquefaciens; D018441:Biofilms; D055666:Lipopeptides; D011550:Pseudomonas aeruginosa
chemical_list: D055666:Lipopeptides
keywords: Anti-biofilm activity; B. amyloliquefaciens; Biofilm formation; Lipopeptide; Pseudomonas aeruginosa