title: Effects of Local Administration of Platelet Rich Plasma on Functional Recovery after Bridging Sciatic Nerve Defect Using Silicone Rubber Chamber; An Experimental Study.
abstract: OBJECTIVE
  To determine the effects of local administration of platelet rich plasma (PRP) on peripheral nerve regeneration in rat sciatic nerve transection model.
  
  
  METHODS
  Forty-five male white Wistar rats were randomized into three experimental groups (n=15): Normal control group (NC), silicon group (SIL), PRP treated group (SIL/PRP). In NC group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In SIL group left sciatic nerve was exposed the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. Proximal and distal stumps were each inserted into a silicone conduit and filled with 10 µL phosphate buffered solution. In SIL/PRP group silicon conduit was filled with 20 µL PRP. Each group was subdivided into three subgroups of five animals each and were studied 4, 8, 12 weeks after surgery.
  
  
  RESULTS
  The animals were comparable regarding the baseline characteristics. Behavioral testing, sciatic nerve functional study and gastrocnemius muscle mass showed earlier regeneration of axons in SIL/PRP than in SIL group.
  
  
  CONCLUSIONS
  Local administration of PRP combined with silicon grafting could accelerate functional recovery of peripheral nerve.  Easily available growth factors and bioactive proteins present in PRP may have clinical implications for the surgical management of patients after nerve transection.
authors.affiliation: Department of Surgery and Diagnostic Imaging, Urmia University, Urmia, Iran.
authors.forename: Sedighe
authors.initials: S
authors.lastname: Abbasipour-Dalivand
authors.affiliation: Department of Surgery and Diagnostic Imaging, Urmia University, Urmia, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: Department of Internal Medicine and Clinical Pathology, Urmia University, Urmia, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Mohammadi
keywords: Functional recovery; Local administration; Peripheral nerve repair; Platelet rich plasma (PRP); Sciatic