title: Expressions of lipoprotein receptors and cholesterol efflux regulatory proteins during luteolysis in bovine corpus luteum.
abstract: The corpus luteum (CL) synthesises and secretes progesterone (P4), which is essential for the establishment and maintenance of pregnancy in mammals. P4 is synthesised from cholesterol. Cholesterol is internalised by low-density lipoprotein receptor (LDLR) and/or scavenger receptor B1 (SR-BI), and is effluxed by ATP-binding cassette (ABC) transporter A1 (ABCA1) and G1 (ABCG1). To test the hypothesis that lipoprotein receptors and ABC transporters are involved in functional luteolysis, we examined the expression of LDLR, SR-BI, ABCA1 and ABCG1 in bovine CL during the luteal stages and after injection of prostaglandin (PG) F2αon Day 10 after ovulation. Expression of LDLR and SR-BI mRNA and protein was lower in the regressed luteal than late luteal stage. Injection of cows with a PGF2αdid not affect LDLR mRNA and protein levels in the CL. Although expression of SR-BI mRNA did not change, SR-BI protein expression decreased 12 and 24h after PGF2αinjection. The overall findings of the present study suggest that the decreased expression of SR-BI induced by PGF2αis one of the factors responsible for the continuous decrease in P4 production during functional luteolysis.
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University, Tsushima Naka Kita-ku 1-1-1, Okayama 700-8530, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Horihata
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Tsushima Naka Kita-ku 1-1-1, Okayama 700-8530, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Yoshioka
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Tsushima Naka Kita-ku 1-1-1, Okayama 700-8530, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sano
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Tsushima Naka Kita-ku 1-1-1, Okayama 700-8530, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Tsushima Naka Kita-ku 1-1-1, Okayama 700-8530, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Skarzynski
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University, Tsushima Naka Kita-ku 1-1-1, Okayama 700-8530, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics* / Q000378:metabolism*; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1 / Q000235:genetics; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism*; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D008183:Luteal Phase / Q000235:genetics / Q000378:metabolism; D003341:Luteolysis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D018110:Receptors, Lipoprotein / Q000235:genetics* / Q000378:metabolism*; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism
chemical_list: D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D012333:RNA, Messenger; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D051122:Scavenger Receptors, Class B; D011374:Progesterone; D015237:Dinoprost