title: Fluorescence Detection of DNA Based on Non-covalent π-π Stacking Interaction between 1-Pyrenebutanoic Acid and Hypericin.
abstract: Herein, we applied hypericin (Hyp) for the detection of DNA on streptavidin-functionalized magnetic beads (SA-MB) through non-covalent π-π stacking interaction between 1-pyrenebutanoic acid (PBA) and Hyp. Briefly, the target DNA (tDNA) was hybridized to morpholino oligonucleotide (MO) that was immobilized on the surface of SA-MB. PBA was incorporated to the backbone of tDNA by means of phosphate-zirconium-carboxylate coordination reaction. Then, fluorescent Hyp was linked to PBA via π-π stacking interaction. Under the optimal conditions, this biosensor displayed a good linear relationship between the fluorescence intensity and the tDNA concentrations in the range from 1 to 100 nM with a low detection limit of 2.9 nM. The mismatch type in a specific DNA sequence can also be efficiently discriminated. Compared with some other reports, this method is more convenient, and has great potential for practical applications.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Hu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Jinming
authors.initials: J
authors.lastname: Kong
mesh_terms: D000873:Anthracenes; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D060526:Magnetic Fields; D015394:Molecular Structure; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D017319:Photosensitizing Agents / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D000873:Anthracenes; D017319:Photosensitizing Agents; D011721:Pyrenes; C002218:1-pyrenebutyrate; D010569:Perylene; C004965:hypericin; D004247:DNA