title: Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants.
abstract: The first transgenes were introduced in a plant genome more than 30 years ago. Since then, the capabilities of the plant scientific community to engineer the genome of plants have progressed at an unparalleled speed. Plant genetic engineering has become a central technology that has dramatically incremented our basic knowledge of plant biology and has enabled the translation of this knowledge into a number of increasingly complex and sophisticated biotechnological applications, which in most cases rely on the simultaneous co-expression of multiple recombinant proteins from different origins. To meet the new challenges of modern plant biotechnology, the plant scientific community has developed a vast arsenal of innovative molecular tools and genome engineering strategies. In this chapter we review a variety of tools, technologies, and strategies developed to transfer and simultaneously co-express multiple transgenes and proteins in a plant host. Their potential advantages, disadvantages, and future prospects are also discussed.
authors.affiliation: Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra-Cerdanyola del Vallès, 08193, Barcelona, Spain. albertferrer@ub.edu.|Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain. albertferrer@ub.edu.
authors.forename: Albert
authors.initials: A
authors.lastname: Ferrer
authors.affiliation: Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra-Cerdanyola del Vallès, 08193, Barcelona, Spain.|Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain.
authors.forename: Monserrat
authors.initials: M
authors.lastname: Arró
authors.affiliation: Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra-Cerdanyola del Vallès, 08193, Barcelona, Spain.|Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Manzano
authors.affiliation: Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra-Cerdanyola del Vallès, 08193, Barcelona, Spain.|Department of Plant Physiology, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Altabella
mesh_terms: D000818:Animals; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D046912:Multiprotein Complexes; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
chemical_list: D046912:Multiprotein Complexes; D010940:Plant Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins
keywords: Chloroplast transformation; Multigene transfer; Multiprotein expression; Plant biotechnology; Plant transformation