title: Resident corneal c-fms(+) macrophages and dendritic cells mediate early cellular infiltration in adenovirus keratitis.
abstract: The cornea contains a heterogeneous population of antigen-presenting cells with the capacity to contribute to immune responses. Adenovirus keratitis is a severe corneal infection with acute and chronic phases. The role of resident corneal antigen-presenting cells in adenovirus keratitis has not been studied. We utilized transgenic MaFIA mice in which c-fms expressing macrophages and dendritic cells can be induced to undergo apoptosis, in a mouse model of adenovirus keratitis. Clinical keratitis and recruitment of myeloperoxidase and CD45(+) cells were diminished in c-fms depleted, adenovirus infected mice, as compared to controls, consistent with a role for myeloid-lineage cells in adenovirus keratitis.
authors.affiliation: Howe Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA, USA.
authors.forename: Mirja
authors.initials: M
authors.lastname: Ramke
authors.affiliation: Howe Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA, USA.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Howe Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA, USA.
authors.forename: Emma Caroline
authors.initials: EC
authors.lastname: Materne
authors.affiliation: Howe Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA, USA.
authors.forename: Jaya
authors.initials: J
authors.lastname: Rajaiya
authors.affiliation: Howe Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA, USA. Electronic address: james_chodosh@meei.harvard.edu.
authors.forename: James
authors.initials: J
authors.lastname: Chodosh
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology / Q000473:pathology*; D000818:Animals; D054360:Chemokine CXCL1 / Q000378:metabolism; D003315:Cornea / Q000166:cytology / Q000276:immunology; D003319:Corneal Stroma / Q000166:cytology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D015828:Eye Infections, Viral / Q000276:immunology / Q000473:pathology* / Q000821:virology; D007634:Keratitis / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism
chemical_list: D054360:Chemokine CXCL1; D016186:Receptor, Macrophage Colony-Stimulating Factor
keywords: Adenovirus; Adenovirus keratitis; Dendritic cell; Epidemic keratoconjunctivitis; Host-pathogen interaction; Leukocyte; MaFIA mice; Macrophage; Neutrophil