title: Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines.
abstract: Poria cocos has a long history of medicinal use in China. Polysaccharides and their derivatives in the medicine exhibit many beneficial biological activities including anticancer, anti-inflammatory, antioxidant and antiviral activities. In this study, a new polysaccharide (PCP-II) was isolated from sclerotium of Poria cocos. Its physico-chemical characters were identified and its adjuvant activity was investigated in mice co-immunized with H1N1 influenza vaccine and hepatitis B surface antigen (HBsAg). The results revealed that PCP-II has a molecular weight of 29.0kDa. It was composed of fucose, mannose, glucose and galactose in molar ration of 1.00:1.63:0.16:6.29 respectively. Pharmacological data demonstrated that PCP-II increased antigen-specific antibody levels in mice immunized with influenza vaccine. PCP-II also elicited anti-HBsAg antibodies at significantly higher titers and generated robust and durable immunity compared to mice immunized with HBsAg-alum following two administrations. PCP-II improved proliferation of splenocytes, stimulated IL-12p70 and TNF-α productions in dendritic cells and macrophages respectively. These results suggested that PCP-II-adjuvanted vaccines enhanced humoral and cellular immunity. PCP-II could be developed as an efficacious adjuvant in human and animal vaccines.
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: wuyajun0922@sina.com.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: lishuaicl@sina.cn.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: lihaixiayes@163.com.
authors.forename: Haixia
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pharmacy, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China. Electronic address: zcz54dr@163.com.
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: majordan@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Ma
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: xn_02080589@aliyun.com.
authors.forename: Xiunan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: wujunhua1971@163.com.
authors.forename: Junhua
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: liukunlu@126.com.
authors.forename: Kunlu
authors.initials: K
authors.lastname: Liu
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: shanjunjie001@126.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Shan
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: wangyuxia1962@hotmail.com.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006514:Hepatitis B Surface Antigens / Q000276:immunology / Q000494:pharmacology*; D017325:Hepatitis B Vaccines / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D056724:Immunity, Humoral / Q000187:drug effects*; D007114:Immunization; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology / Q000494:pharmacology*; D018664:Interleukin-12 / Q000276:immunology; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D000070660:Wolfiporia / Q000737:chemistry*
chemical_list: D062610:Fungal Polysaccharides; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007252:Influenza Vaccines; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12
keywords: Adjuvant; Polysaccharide; Poria cocos; Vaccine