title: Structure and Activities of the Eukaryotic RNA Exosome.
abstract: The composition of the multisubunit eukaryotic RNA exosome was described more than a decade ago, and structural studies conducted since that time have contributed to our mechanistic understanding of factors that are required for 3'-to-5' RNA processing and decay. This chapter describes the organization of the eukaryotic RNA exosome with a focus on presenting results related to the noncatalytic nine-subunit exosome core as well as the hydrolytic exo- and endoribonuclease Rrp44 (Dis3) and the exoribonuclease Rrp6. This is achieved in large part by describing crystal structures of Rrp44, Rrp6, and the nine-subunit exosome core with an emphasis on how these molecules interact to endow the RNA exosome with its catalytic activities.
authors.affiliation: Structural Biology Program, Sloan-Kettering Institute, New York, USA; Louis V. Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, USA.
authors.forename: Elizabeth V
authors.initials: EV
authors.lastname: Wasmuth
authors.affiliation: Structural Biology Program, Sloan-Kettering Institute, New York, USA. Electronic address: limac@mskcc.org.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Lima
keywords: 3′-to-5′ RNA decay; Cold-shock domain; Endoribonuclease; Exoribonuclease; Exosome; Hydrolytic; KH domain; Multisubunit; PH domain; PIN domain; Phosphorolytic; RNA binding; RNA degradation; RNA processing; RNase; S1 domain; Structure