title: Alphaherpesvirus DNA replication in dissociated human trigeminal ganglia.
abstract: Analysis of the frequency and PCR-quantifiable abundance of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) DNA in multiple biological replicates of cells from dissociated randomly distributed human trigeminal ganglia (TG) of four subjects revealed an increase in both parameters and in both viruses during 5 days of culture, with no further change by 10 days. Dissociated TG provides a platform to analyze initiation of latent virus DNA replication within 5 days of culture.
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA. randall.cohrs@ucdenver.edu.|Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, 80045, USA. randall.cohrs@ucdenver.edu.
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Cohrs
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
authors.forename: Hussain
authors.initials: H
authors.lastname: Badani
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Bos
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Scianna
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
authors.forename: Ian
authors.initials: I
authors.lastname: Hoskins
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
authors.forename: Nicholas L
authors.initials: NL
authors.lastname: Baird
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th Avenue, Mail Stop B182, Aurora, CO, 80045, USA.|Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, 80045, USA.
authors.forename: Don
authors.initials: D
authors.lastname: Gilden
mesh_terms: D000293:Adolescent; D000328:Adult; D001344:Autopsy; D004261:DNA Replication; D005260:Female; D018259:Herpesvirus 1, Human / Q000502:physiology*; D014645:Herpesvirus 3, Human / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D046509:Tissue Culture Techniques; D012668:Trigeminal Ganglion / Q000821:virology*; D014775:Virus Activation; D017735:Virus Latency; D014779:Virus Replication
keywords: DNA; HSV-1; Human trigeminal ganglia; Reactivation; VZV