title: Identification and characterization of a library of surfactins and fengycins from a marine endophytic Bacillus sp.
abstract: An endophytic bacterial strain from a marine green alga, Ulva lactuca, was isolated and identified by 16S rRNA gene sequencing method. The bacterial isolate was found to secrete two major families of cyclic depsilipopeptides, surfactins, and fengycins. Sequencing of the isolated lipopeptides was carried out using the MSndata obtained from an electrospray ionization (ESI) ion trap mass spectrometer coupled to an HPLC system. The assigned sequences were confirmed by a chemical derivatization approach involving esterification followed by mass spectrometric analysis. Distinction of leucine residues from isoleucine was established through a combined electron transfer dissociation-collision-induced dissociation (ETD-CID) method. The fengycins described in this study were found to cause significant delay of growth of two plants, Vigna radiata (mung bean) and Oryza sativa (rice). To the best of our knowledge, this is the first study describing identification and characterization of cyclic peptides from an endophytic Bacillus sp. isolated from marine algae.
authors.affiliation: Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana P.O. Kollam, Kerala, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Nair
authors.affiliation: Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana P.O. Kollam, Kerala, India.
authors.forename: Muralidharan
authors.initials: M
authors.lastname: Vanuopadath
authors.affiliation: Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana P.O. Kollam, Kerala, India.
authors.forename: Bipin G
authors.initials: BG
authors.lastname: Nair
authors.affiliation: Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana P.O. Kollam, Kerala, India.
authors.forename: Jayashree Gopalakrishna
authors.initials: JG
authors.lastname: Pai
authors.affiliation: Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Clappana P.O. Kollam, Kerala, India.
authors.forename: Sudarslal Sadasivan
authors.initials: SS
authors.lastname: Nair
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D060026:Endophytes / Q000302:isolation & purification; D015723:Gene Library; D020459:Genes, rRNA; D055666:Lipopeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000254:growth & development; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry* / Q000302:isolation & purification; D044445:Ulva / Q000382:microbiology; D000070658:Vigna / Q000187:drug effects / Q000254:growth & development
chemical_list: D055666:Lipopeptides; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents; C049972:fengycin; C009365:surfactin peptide
keywords: Collision-induced dissociation; Cyclic depsipeptide; Cyclic lipopeptide; Electron transfer dissociation; Marine endophyte; Mass spectrometry