title: Toxicological evaluation of essential oil from the leaves of Croton tetradenius (Euphorbiaceae) on Aedes aegypti and Mus musculus.
abstract: For control of Aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. Herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of Croton tetradenius on Ae. aegypti larvae and adults. We also evaluated the acute toxicity in Mus musculus. The essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. Female mice were used for assessing toxicity according to the Organization for Economic Cooperation and Development's Test Guideline 423/2001. Doses administered to mice orally and intraperitoneally were 5, 50, 300, and 2000 mg kg(-1). There was a greater toxic effect on larvae (LC50 = 0.152 mg mL(-1) and LC90 = 0.297 mg mL(-1)) and on adults (LC50 = 1.842 mg mL(-1) and LC90 = 3.156 mg mL(-1)) of Ae. aegypti after 24 h of exposure, when compared to other periods of exposure. Chemical analysis revealed 26 components, with camphor (25.49 %) as the major component. The acute toxicity via the intraperitoneal route identified an LD50 = 200 mg kg(-1) and by the oral route an LD50 = 500 mg kg(-1). Thus, the essential oil of C. tetradenius presents insecticidal potential for Ae. aegypti and has high safety threshold at the concentrations evaluated in this study.
authors.affiliation: Mestranda em Ciências Ambientais - Programa de Pós-Graduação em Ciências Ambientais (PPGCA), Universidade Estadual do Sudoeste da Bahia (UESB), Itapetinga, BA, Brazil. karine.lobo@hotmail.com.
authors.forename: Karine da Silva
authors.initials: Kda S
authors.lastname: Carvalho
authors.affiliation: Núcleo de Pesquisa em Química Aplicada/UESB, Praça Primavera, 40, Bairro Primavera, 45700-000, Itapetinga, BA, Brazil.
authors.forename: Sandra Lúcia da Cunha
authors.initials: SL
authors.lastname: E Silva
authors.affiliation: Departamento de Antibióticos, Universidade Federal de Pernambuco, Av. Arthur de Sá, s/n, 50740-521, Recife, PE, Brazil.
authors.forename: Ivone Antonia
authors.initials: IA
authors.lastname: de Souza
authors.affiliation: Núcleo de Pesquisa em Química Aplicada/UESB, Praça Primavera, 40, Bairro Primavera, 45700-000, Itapetinga, BA, Brazil.
authors.forename: Simone Andrade
authors.initials: SA
authors.lastname: Gualberto
authors.affiliation: Núcleo de Pesquisa em Química Aplicada/UESB, Praça Primavera, 40, Bairro Primavera, 45700-000, Itapetinga, BA, Brazil.
authors.forename: Rômulo Carlos Dantas
authors.initials: RC
authors.lastname: da Cruz
authors.affiliation: Departamento de Química/ICE, Universidade Federal Rural do Rio de Janeiro/UFRRJ, Br 465, Km 7 Seropédica, 23890-000, Rio de Janeiro, RJ, Brazil.
authors.forename: Frances Regiane
authors.initials: FR
authors.lastname: Dos Santos
authors.affiliation: Departamento de Química/ICE, Universidade Federal Rural do Rio de Janeiro/UFRRJ, Br 465, Km 7 Seropédica, 23890-000, Rio de Janeiro, RJ, Brazil.
authors.forename: Mário Geraldo
authors.initials: MG
authors.lastname: de Carvalho
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D029802:Croton / Q000737:chemistry*; D005260:Female; D007306:Insecticides / Q000302:isolation & purification / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D051379:Mice; D009822:Oils, Volatile / Q000302:isolation & purification / Q000633:toxicity*; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D007306:Insecticides; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: Acute toxicity; Caatinga; Plants; Vector control