title: Brownian Dynamics of Electrostatically Adhering Small Vesicles to a Membrane Surface Induces Domains and Probes Viscosity.
abstract: Using single-particle tracking, we investigate the interaction of small unilamellar vesicles (SUVs) that are electrostatically tethered to the freestanding membrane of a giant unilamellar vesicle (GUV). We find that the surface mobility of the GUV-riding SUVs is Brownian, insensitive to the bulk viscosity, vesicle size, and vesicle fluidity but strongly altered by the viscosity of the underlying membrane. Analyzing the diffusional behavior of SUVs within the Saffman-Delbrück model for the dynamics of membrane inclusions supports the notion that the mobility of the small vesicles is coupled to that of dynamically induced lipid clusters within the target GUV membrane. The reversible binding also offers a nonperturbative means for measuring the viscosity of biomembranes, which is an important parameter in cell physiology and function.
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Drive 637553, Singapore.
authors.forename: Seyed R
authors.initials: SR
authors.lastname: Tabaei
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Drive 637553, Singapore.
authors.forename: Jurriaan J J
authors.initials: JJ
authors.lastname: Gillissen
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Drive 637553, Singapore.
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Kim
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Drive 637553, Singapore.
authors.forename: James C S
authors.initials: JC
authors.lastname: Ho
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Drive 637553, Singapore.
authors.forename: Bo
authors.initials: B
authors.lastname: Liedberg
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Drive 637553, Singapore.|Department of Biomedical Engineering and Department of Chemical Engineering and Materials Science, University of California , Davis, California 95616, United States.
authors.forename: Atul N
authors.initials: AN
authors.lastname: Parikh
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University , 50 Nanyang Drive 637553, Singapore.|School of Chemical and Biomedical Engineering, Nanyang Technological University , 62 Nanyang Drive 637459, Singapore.
authors.forename: Nam-Joon
authors.initials: NJ
authors.lastname: Cho