title: Evaluation of the Tosoh G8 Analyzer and Comparison with the Trinity Biotech Premier Hb9210 Analyzer for the Measurement of HbA₁c.
abstract: BACKGROUND
  The aim of our study was to assess the analytical performance of the Tosoh HLC-723G8 automated analyzer and to compare it with the Trinity Biotech Premier Hb9210 analyzer for the measurement of hemoglobin A₁c (HbA₁c).
  
  
  METHODS
  A total of 101 patients with pre-diabetes or diabetes mellitus were included in the study. HbA₁c, was measured by both an ion-exchange high-performance liquid chromatography (IE-HPLC) method and a boronate affinity chromatography method. Statistical analysis was performed using Deming regression. Bland-Altman plots were used to calculate mean difference (bias).
  
  
  RESULTS
  The CV% values of IE-HPLC and boronate affinity methods for within run and between days were lower than 2.0%. High correlation was found (y = 1.0045x + 0.2111; r = 0.9941) between the two methods. The method shows no interference from carbamylated hemoglobin.
  
  
  CONCLUSIONS
  Both systems showed acceptable performance and are suitable for clinical application in the analysis of HbA₁c. However, laboratories should be aware of the limitations of their methods and the availability of more accurate and precise HbA₁c, determination methods.
authors.forename: Fatma
authors.initials: F
authors.lastname: Ucar
authors.forename: Gonul
authors.initials: G
authors.lastname: Erden
authors.forename: Seyda
authors.initials: S
authors.lastname: Ozdemir
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Yildiz
authors.forename: Abdullah Ercan
authors.initials: AE
authors.lastname: Arzuhal
authors.forename: Ismail
authors.initials: I
authors.lastname: Temel
mesh_terms: D000328:Adult; D000368:Aged; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006403:Hematologic Tests / Q000295:instrumentation*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human