title: [Establishment and validation of a neonatal pig model of hemolytic jaundice].
abstract: OBJECTIVE
  To establish a neonatal pig model of hemolytic jaundice.
  
  
  METHODS
  Twelve seven-day-old purebred Yorkshire pigs were randomly divided into an experimental group and a control group (n=6 each). Immunization of New Zealand white rabbits was used to prepare rabbit anti-porcine red blood cell antibodies, and rabbit anti-porcine red blood cell serum was separated. The neonatal pigs in the experimental group were given an intravenous injection of rabbit anti-porcine red blood cell serum (5 mL), and those in the control group were given an intravenous injection of normal saline (5 mL). Venous blood samples were collected every 6 hours for routine blood test and liver function evaluation.
  
  
  RESULTS
  The experimental group had a significantly higher serum bilirubin level than the control group at 18 hours after the injection of rabbit anti-porcine red blood cell serum (64±30 μmol/L vs 20±4 μmol/L; P<0.05). In the experimental group, the serum bilirubin level reached the peak at 48 hours (275±31 μmol/L), and decreased significantly at 96 hours after the injection (95±17 μmol/L), but all significantly higher than that in the control group (P<0.05). At 18 hours after the injection, the experimental group had a significantly lower red blood cell (RBC) count than the control group [(4.58±0.32)×10(12)/L vs (5.09±0.44)×10(12)/L; P<0.05]; at 24 hours, the experimental group showed further reductions in RBC count and hemoglobin level and had significantly lower RBC count and hemoglobin level than the control group [RBC: (4.21±0.24)×10(12)/L vs (5.11±0.39)×10(12)/L, P<0.05; hemoglobin: 87±3 g vs 97±6 g, P<0.05]. The differences in RBC count and hemoglobin level between the two groups were largest at 36-48 hours.
  
  
  CONCLUSIONS
  The neonatal pig model of hemolytic jaundice simulates the pathological process of human hemolytic jaundice well and provides good biological and material bases for further investigation of neonatal hemolysis.
authors.affiliation: Department of Neonatology, Suzhou Municipal Hospital, Suzhou, Jiangsu 215002, China. wangsn@126.com.
authors.forename: Yong-Fu
authors.initials: YF
authors.lastname: Li
authors.forename: Yue-Lan
authors.initials: YL
authors.lastname: Ma
authors.forename: Ling
authors.initials: L
authors.lastname: Nie
authors.forename: Shuan
authors.initials: S
authors.lastname: Chen
authors.forename: Mei-Fang
authors.initials: MF
authors.lastname: Jin
authors.forename: San-Lan
authors.initials: SL
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001663:Bilirubin / Q000097:blood; D004195:Disease Models, Animal; D004906:Erythrocyte Count; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D007565:Jaundice / Q000209:etiology*; D008297:Male; D011817:Rabbits; D013552:Swine
chemical_list: D006454:Hemoglobins; D001663:Bilirubin