title: Biological influence of Ca/P ratio on calcium phosphate coatings by sol-gel processing.
abstract: The objective of this work has been to develop low temperature sol-gel glass coatings to modify the substrate surface and to evaluate their bioactivity and biocompatibility. Glasses, based on SiO2·CaO·P2O5, were synthesized by the sol-gel technique using tetraethyl orthosilicate, calcium nitrate tetrahydrate and triethyl phosphate as precursors of SiO2, CaO and P2O5, respectively. Those materials, still in the sol phase, have been used to coat substrates by means of the dip-coating technique. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) has been used for characterize coatings and a microstructural analysis has been obtained using scanning electron microscopy (SEM). The potential applications of the coatings in the biomedical field were evaluated by bioactivity and biocompatibility tests. The coated substrate was immersed in simulated body fluid (SBF) for 21days and the hydroxyapatite deposition on its surface was subsequently evaluated via SEM-EDXS analysis, as an index of bone-bonding capability. In order to study the cell behavior and response to our silica based materials, prepared via the sol-gel method, with various Ca/P ratio and coating substrate, we have used the human osteoblast-like U2OS cell line.
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, Italy. Electronic address: michelina.catauro@unina2.it.
authors.forename: M
authors.initials: M
authors.lastname: Catauro
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Papale
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via L. De Crecchio 7, 80138 Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Sapio
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via L. De Crecchio 7, 80138 Naples, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Naviglio
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D017610:Calcium Compounds / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry; D005782:Gels / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009566:Nitrates / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D017553:Phosphorus Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D005782:Gels; D009566:Nitrates; D010087:Oxides; D017553:Phosphorus Compounds; C012500:phosphorus pentoxide; D012822:Silicon Dioxide; D017886:Durapatite; C016538:lime; C059948:calcium nitrate
keywords: Bioactivity test; Cytotoxicity evaluation; SiO(2)-CaO-P(2)O(5) glasses; Sol–gel synthesis