title: The role of adrenergic activation on murine luteal cell viability and progesterone production.
abstract: Sympathetic innervations exist in mammalian CL. The action of catecholaminergic system on luteal cells has been the focus of a variety of studies. Norepinephrine (NE) increased progesterone secretion of cattle luteal cells by activating β-adrenoceptors. In this study, murine luteal cells were treated with NE and isoprenaline (ISO). We found that NE increased the viability of murine luteal cells and ISO decreased the viability of luteal cells. Both NE and ISO promoted the progesterone production. Nonselective β-adrenergic antagonist, propranolol reversed the effect of ISO on cell viability but did not reverse the effect of NE on cell viability. Propranolol blocked the influence of NE and ISO on progesterone production. These results reveal that the increase of luteal cell viability induced by NE is not dependent on β-adrenergic activation. α-Adrenergic activation possibly contributes to it. Both NE and ISO increased progesterone production through activating β-adrenergic receptor. Further study showed that CyclinD2 is involved in the increase of luteal cell induced by NE. 3β-Hydroxysteroid dehydrogenase, LHR, steroidogenic acute regulatory protein (StAR), and PGF2α contribute to the progesterone production induced by NE and ISO.
authors.affiliation: Department of Microbiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Huaide
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Hu
authors.affiliation: Joint programme of Nanchang University and Queen Mary University of London, London, UK.
authors.forename: Riqiang
authors.initials: R
authors.lastname: Bao
authors.affiliation: Joint programme of Nanchang University and Queen Mary University of London, London, UK.
authors.forename: Qiming
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Cell Biology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: zhangcp81@gmail.com.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000494:pharmacology; D018663:Adrenergic Agents / Q000494:pharmacology*; D000818:Animals; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival; D056742:Cyclin D2 / Q000235:genetics / Q000378:metabolism; D015237:Dinoprost / Q000235:genetics / Q000378:metabolism; D005260:Female; D007545:Isoproterenol / Q000008:administration & dosage / Q000494:pharmacology; D008184:Luteal Cells / Q000187:drug effects*; D051379:Mice; D009638:Norepinephrine / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology
chemical_list: D000199:Actins; D018663:Adrenergic Agents; C486196:Ccnd2 protein, mouse; D056742:Cyclin D2; D011374:Progesterone; D011433:Propranolol; D015237:Dinoprost; D053148:Caspase 3; D007545:Isoproterenol; D009638:Norepinephrine
keywords: Isoprenaline; Luteal cell; Norepinephrine; Progesterone