title: Lipidomics Analyses Reveal Temporal and Spatial Lipid Organization and Uncover Daily Oscillations in Intracellular Organelles.
abstract: Cells have evolved mechanisms to handle incompatible processes through temporal organization by circadian clocks and by spatial compartmentalization within organelles defined by lipid bilayers. Recent advances in lipidomics have led to identification of plentiful lipid species, yet our knowledge regarding their spatiotemporal organization is lagging behind. In this study, we quantitatively characterized the nuclear and mitochondrial lipidome in mouse liver throughout the day, upon different feeding regimens, and in clock-disrupted mice. Our analyses revealed potential connections between lipid species within and between lipid classes. Remarkably, we uncovered diurnal oscillations in lipid accumulation in the nucleus and mitochondria. These oscillations exhibited opposite phases and readily responded to feeding time. Furthermore, we found that the circadian clock coordinates the phase relation between the organelles. In summary, our study provides temporal and spatial depiction of lipid organization and reveals the presence and coordination of diurnal rhythmicity in intracellular organelles.
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Rona
authors.initials: R
authors.lastname: Aviram
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Gal
authors.initials: G
authors.lastname: Manella
authors.affiliation: Department of Biological Services, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Naama
authors.initials: N
authors.lastname: Kopelman
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Adi
authors.initials: A
authors.lastname: Neufeld-Cohen
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Ziv
authors.initials: Z
authors.lastname: Zwighaft
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Meytar
authors.initials: M
authors.lastname: Elimelech
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Yaarit
authors.initials: Y
authors.lastname: Adamovich
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel.
authors.forename: Marina
authors.initials: M
authors.lastname: Golik
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL 32827, USA.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL 32827, USA.
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel. Electronic address: gad.asher@weizmann.ac.il.
authors.forename: Gad
authors.initials: G
authors.lastname: Asher
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002940:Circadian Rhythm / Q000235:genetics; D005247:Feeding Behavior; D005838:Genotype; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D018345:Mice, Knockout; D008930:Mitochondria, Liver / Q000378:metabolism*; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism; D010507:Periodicity; D010641:Phenotype; D013997:Time Factors
chemical_list: C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins