title: Production of an anti-Candida peptide via fed batch and ion exchange chromatography.
abstract: Interest in peptides as diagnostic and therapeutic materials require their manufacture via either a recombinant or synthetic route. This study examined the former, where a recombinant fusion consisting of an antifungal peptide was expressed and isolated from Escherichia coli. Fed batch fermentation with E. coli harboring an arabinose-inducible plasmid produced the 12 residue anti-Candida peptide fused to the N-terminal of Green Fluorescent Protein (GFPUV ). The purification of the fusion protein, using ion-exchange chromatography, was monitored by using the intrinsic fluorescence of GFPUV . The recombinant antifungal peptide was successfully released by cyanogen bromide-induced cleavage of the fusion protein. The recombinant peptide showed the expected antifungal activity. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:865-871, 2016.
authors.affiliation: Ralph E. Martin Dept. of Chemical Engineering, University of Arkansas, Fayetteville, AR.
authors.forename: Rudra Palash
authors.initials: RP
authors.lastname: Mukherjee
authors.affiliation: Ralph E. Martin Dept. of Chemical Engineering, University of Arkansas, Fayetteville, AR.
authors.forename: Robert
authors.initials: R
authors.lastname: Beitle
authors.affiliation: Dept. of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Jayanthi
authors.affiliation: Dept. of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR.
authors.forename: T K S
authors.initials: TK
authors.lastname: Kumar
authors.affiliation: Dept. of Biological Sciences, University of Arkansas, Fayetteville, AR.
authors.forename: David S
authors.initials: DS
authors.lastname: McNabb
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques; D002176:Candida albicans / Q000187:drug effects*; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D010455:Peptides; D011994:Recombinant Proteins
keywords: antifungal peptide; cyanogen bromide; fed batch