title: Site-directed spin labeling of proteins for distance measurements in vitro and in cells.
abstract: Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.
authors.affiliation: Department of Chemistry, Zukunftskolleg, and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany. malte.drescher@uni-konstanz.de.
authors.forename: P
authors.initials: P
authors.lastname: Roser
authors.affiliation: Department of Chemistry, Zukunftskolleg, and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany. malte.drescher@uni-konstanz.de.
authors.forename: M J
authors.initials: MJ
authors.lastname: Schmidt
authors.affiliation: Department of Chemistry, Zukunftskolleg, and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany. malte.drescher@uni-konstanz.de.
authors.forename: M
authors.initials: M
authors.lastname: Drescher
authors.affiliation: Department of Chemistry and Chemical Biology, Technical University of Dortmund, Otto-Hahn-Str. 6, 44227 Dortmund, Germany. daniel.summerer@tu-dortmund.de.
authors.forename: D
authors.initials: D
authors.lastname: Summerer
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D011506:Proteins; D013113:Spin Labels