title: Mg(2+)-Dependent Control of the Spatial Arrangement of Rhodococcus erythropolis PR4 Cells in Aqueous-Alkane Two Phase Culture Containing n-Dodecane.
abstract: We recently reported that a close relationship exists between alkane carbon-chain length, cell growth, and translocation frequency in Rhodococcus. In the present study, we examined the regulation of the spatial arrangement of cells in aqueous-alkane two phase cultures. An analysis of the effects of minerals on cell localization revealed that changes in the concentration of MgSO4 in two phase cultures containing n-dodecane (C12) altered cell localization from translocation to adhesion and vice versa. Our results indicate that the spatial arrangement of cells in two phase culture systems is controlled through the regulation of MgSO4 concentrations.
authors.affiliation: Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University.
authors.forename: Hayato
authors.initials: H
authors.lastname: Takihara
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Akase
authors.forename: Michio
authors.initials: M
authors.lastname: Sunairi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Iwabuchi
mesh_terms: D000473:Alkanes / Q000378:metabolism*; D001422:Bacterial Adhesion; D002273:Carcinogens / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D008278:Magnesium Sulfate / Q000378:metabolism*; D012240:Rhodococcus / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology
chemical_list: D000473:Alkanes; D002273:Carcinogens; D003470:Culture Media; C007548:n-dodecane; D008278:Magnesium Sulfate