title: Monitoring microstructural variations of fresh skeletal muscle tissues by Mueller matrix imaging.
abstract: Recently many attempts have been made for extracting the structural information of myofibrils as indicators for diseases of skeletal muscle. In this paper we adopt wide-field illumination and take the backscattering Mueller matrix images of bovine skeletal muscle tissues during the 24-hour experimental time after the animal's death. The 2D images of Mueller matrix elements and their frequency distribution histograms (FDHs) reveal rich qualitative information on the changes in the microstructures of the skeletal muscle. The temporal variations of the sample are quantitatively analyzed using two Mueller matrix transformation (MMT) parameters. The characteristic features of the temporal plots are attributed to the rigor mortis and proteolysis processes. For a deeper insight on the relationship between the features of the MMT parameters and the microstructures during the rigor mortis and proteolysis processes, Monte Carlo (MC) simulations are carried out based on sphere-cylinder birefringence model (SCBM). The good agreement between the experimental and MC simulated results show that the FDHs and MMT parameters can describe more clearly the characteristic microstructural features of skeletal muscle tissues. The techniques are useful for the characterization of physiological status of tissues, or quantitative assessment of meat qualities in food industry.
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
authors.forename: Honghui
authors.initials: H
authors.lastname: He
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.|Department of Biomedical Engineering, Tsinghua University, Beijing, 100084, China.
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.|Department of Physics, Tsinghua University, Beijing, 100084, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Chang
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.|Department of Biomedical Engineering, Tsinghua University, Beijing, 100084, China.
authors.forename: Donghong
authors.initials: D
authors.lastname: Lv
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
authors.forename: Chaijie
authors.initials: C
authors.lastname: Duan
authors.affiliation: Division of Advanced Manufacturing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zeng
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: He
authors.affiliation: Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.|Department of Physics, Tsinghua University, Beijing, 100084, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ma
mesh_terms: D000818:Animals; D001718:Birefringence; D002417:Cattle; D007090:Image Interpretation, Computer-Assisted; D008027:Light; D008853:Microscopy / Q000379:methods*; D009010:Monte Carlo Method; D018482:Muscle, Skeletal / Q000648:ultrastructure*; D012542:Scattering, Radiation
keywords: Monte Carlo simulations ; Mueller matrix; microstructure; polarization; scattering