title: A simultaneous two-colour detection method of human IgG- and IgE-reactive proteins from lactic acid bacteria.
abstract: Whole cell extracts of two Lactobacillus strains were tested with primary antibodies from two pooled sera from allergic patients. Fluorescently labelled anti-human IgG and anti-human IgE secondary antibodies applied in Western blotting, together with an appropriate image acquisition protocol facilitated imagining bacterial proteins that reacted with human IgG and IgE.
authors.affiliation: Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Str. 10, 10-748 Olsztyn, Poland. Electronic address: l.markiewicz@pan.olsztyn.pl.
authors.forename: Lidia Hanna
authors.initials: LH
authors.lastname: Markiewicz
authors.affiliation: Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Str. 10, 10-748 Olsztyn, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Szymkiewicz
authors.affiliation: Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Str. 10, 10-748 Olsztyn, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Szyc
authors.affiliation: Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Str. 10, 10-748 Olsztyn, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Wróblewska
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D015153:Blotting, Western / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D006967:Hypersensitivity; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007778:Lactobacillus / Q000737:chemistry*; D007780:Lacticaseibacillus casei / Q000737:chemistry; D048668:Lactobacillus delbrueckii / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007073:Immunoglobulin E
keywords: Bacterial proteins; Human serum; IgE; IgG; Immunoreactivity; Lactobacillus