title: Biolistic transformation of Scoparia dulcis L.
abstract: Here, we report for the first time, the optimized conditions for microprojectile bombardment-mediated genetic transformation in Vassourinha (Scoparia dulcis L.), a Plantaginaceae medicinal plant species. Transformation was achieved by bombardment of axenic leaf segments with Binary vector pBI121 harbouring β-glucuronidase gene (GUS) as a reporter and neomycin phosphotransferase II gene (npt II) as a selectable marker. The influence of physical parameters viz., acceleration pressure, flight distance, gap width & macroprojectile travel distance of particle gun on frequency of transient GUS and stable (survival of putative transformants) expressions have been investigated. Biolistic delivery of the pBI121 yielded the best (80.0 %) transient expression of GUS gene bombarded at a flight distance of 6 cm and rupture disc pressure/acceleration pressure of 650 psi. Highest stable expression of 52.0 % was noticed in putative transformants on RMBI-K medium. Integration of GUS and npt II genes in the nuclear genome was confirmed through primer specific PCR. DNA blot analysis showed more than one transgene copy in the transformed plantlet genomes. The present study may be used for metabolic engineering and production of biopharmaceuticals by transplastomic technology in this valuable medicinal plant.
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Kota
authors.initials: K
authors.lastname: Srinivas
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Narra
authors.initials: N
authors.lastname: Muralikrishna
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Kalva Bharath
authors.initials: KB
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Ellendula
authors.initials: E
authors.lastname: Raghu
authors.affiliation: Department of Biotechnology, Telangana University, Nizamabad, India.
authors.forename: Aileni
authors.initials: A
authors.lastname: Mahender
authors.affiliation: Department of Biotechnology, Telangana University, Nizamabad, India.
authors.forename: Kasula
authors.initials: K
authors.lastname: Kiranmayee
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Velivela
authors.initials: V
authors.lastname: Yashodahara
authors.affiliation: Department of Biotechnology, Kakatiya University, Warangal, 506009 India.
authors.forename: Abbagani
authors.initials: A
authors.lastname: Sadanandam
keywords: Axenic leaf explants; Biolistic transformation; GUS assay; Scoparia dulcis L.; Stable expression