title: SPOP Regulates Endometrial Stromal Cell Decidualization in Mice.
abstract: Ubiquitination is a regulatory mechanism that occurs after protein translation. To date, few studies have reported on ubiquitination during embryo implantation. We used real-time quantitative polymerase chain reaction, immunohistochemistry, and Western blotting analyses to analyze the expression of speckle-type pox virus and zinc finger (POZ) protein (SPOP; an adapter of E3 ligases of ubiquitination) in mouse uteri during early pregnancy and pseudopregnancy using an artificially induced decidualization model and a steroid hormone-processing model. At the same time, we established an artificially induced decidualization in vitro model. We observed that SPOP regulates endometrial stromal cell decidualization in mice and that hormones regulate the expression of SPOP. This study suggests that ubiquitination may be involved in embryonic implantation.
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Molecular Medical Laboratory, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Qiubo
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Rufei
authors.initials: R
authors.lastname: Gao
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China.
authors.forename: Yingxiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Yuzhong District, Chongqing, People's Republic of China cqmu_liuxueqing@126.com.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003656:Decidua / Q000378:metabolism / Q000502:physiology; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism / Q000502:physiology*; D004967:Estrogens / Q000008:administration & dosage / Q000502:physiology; D005260:Female; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000008:administration & dosage / Q000502:physiology; D011388:Prolactin / Q000031:analogs & derivatives / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism / Q000502:physiology*; D017154:Stromal Cells / Q000378:metabolism / Q000502:physiology*; D043743:Ubiquitin-Protein Ligase Complexes; D054875:Ubiquitination
chemical_list: C489796:Dtprp protein, mouse; D004967:Estrogens; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D011374:Progesterone; D011388:Prolactin; C509762:Spop protein, mouse; D043743:Ubiquitin-Protein Ligase Complexes
keywords: SPOP; decidualization; hormones; ubiquitination