title: An ultra-facile and label-free immunoassay strategy for detection of copper (II) utilizing chemiluminescence self-enhancement of Cu (II)-ethylenediaminetetraacetate chelate.
abstract: The establishment of facile, rapid, sensitive and cost-effective protocols for the detection of heavy metals is of great significance for human health and environmental monitoring. Hereby, an ultra-facile and label-free immunoassay strategy was designed for detecting heavy metal ion by using Cu (II) as the model analyte. Cu (II) reacted previously with ethylenediaminetetraacetate (EDTA) was captured by immobilized monoclonal antibody for Cu (II)-EDTA chelate. Then Cu (II) was detected based on the self-enhancing effect of Cu (II)-EDTA chelate to luminol-H2O2 chemiluminescence reaction. The CL intensity is linear relative with Cu (II) concentration in a very wide range of 1.0-1000ng/mL, with a detection limit of 0.33ng/mL (S/N=3). Since the specificity of this proposed strategy relied on both the specificity of monoclonal antibody and the specificity of luminol-H2O2 system, it could avoid interference from most common ions. The proposed method was used successfully to detect Cu (II) in traditional Chinese medicine and environmental water samples with acceptable recovery values of 82-113%. This proof-of-principle work demonstrated the feasibility of the label-free immunoassay for heavy metal ions, and opened a new avenue for rapid screening and field assay for drug safety, environmental monitoring and clinical diagnosis.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shu
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: fuzf@swu.edu.cn.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods; D002413:Cations, Divalent / Q000032:analysis; D003300:Copper / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004492:Edetic Acid / Q000737:chemistry*; D004784:Environmental Monitoring / Q000191:economics / Q000379:methods; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D060106:Lakes / Q000032:analysis*; D049449:Luminescence; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods*; D008165:Luminol / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D002413:Cations, Divalent; D004365:Drugs, Chinese Herbal; D008165:Luminol; D003300:Copper; D004492:Edetic Acid; D006861:Hydrogen Peroxide
keywords: Chelate; Chemiluminescence; Copper (II); Ethylenediaminetetraacetate; Immunoassay