title: Synthesis and characterization of imprinted sorbent for separation of gramine from bovine serum albumin.
abstract: The aim of this study was to develop an efficient sorbent for separation of N,N-dimethyl-3-aminomethylindole (gramine) from bovine serum albumin. An imprinting technology was involved in the synthesis of polymers from nine different functional monomers in the presence of ethylene glycol dimethacrylate as a cross-linker. The analysis of binding capacities showed that the highest specificity towards gramine was achieved when 4-vinylbenzoic acid was used as the functional monomer in methanol to form the bulk imprinted polymer, MIP1 (imprinting factor equal to 21.3). The Scatchard analysis of MIP1 showed two classes of binding sites with the dissociation constants Kd equal to 0.105 and 6.52μmolL(-1). The composition and morphology of polymers were defined by (13)C CP/MAS NMR, BET and SEM-EDS analyses. The recognition mechanism of MIP1 was tested using the structurally related bioanalytes, and the dominant role of indole moiety and ethylamine side chain was revealed. A new MISPE protocol was optimized for separation of gramine. The total recoveries on MIP1 were equal to 94±12 % from standard solutions and 85±11 % from bovine serum albumin.
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Luliński
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Klejn
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland. Electronic address: dmaciejewska@wum.edu.pl.
authors.forename: Dorota
authors.initials: D
authors.lastname: Maciejewska
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D026121:Indole Alkaloids; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D052616:Solid Phase Extraction; D013343:Styrenes / Q000737:chemistry
chemical_list: D000470:Alkaloids; D026121:Indole Alkaloids; D011108:Polymers; D013343:Styrenes; D012710:Serum Albumin, Bovine; C007884:gramine; C105064:4-vinylbenzoic acid
keywords: Gramine; Molecularly imprinted polymer; Solid phase extraction