title: Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I.
abstract: Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes is crucial for proper cell division. The mechanisms that facilitate kinetochore capture by microtubules are still unclear. In the present study, we combine experiments and theory to explore the mechanisms of kinetochore capture at the onset of meiosis I in fission yeast. We show that kinetochores on homologous chromosomes move together, microtubules are dynamic and pivot around the spindle pole, and the average capture time is 3-4 minutes. Our theory describes paired kinetochores on homologous chromosomes as a single object, as well as angular movement of microtubules and their dynamics. For the experimentally measured parameters, the model reproduces the measured capture kinetics and shows that the paired configuration of kinetochores accelerates capture, whereas microtubule pivoting and dynamics have a smaller contribution. Kinetochore pairing may be a general feature that increases capture efficiency in meiotic cells.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Gheorghe
authors.initials: G
authors.lastname: Cojoc
authors.affiliation: Max Planck Institute for the Physics of Complex Systems, Nöthnitzerstr. 38, 01187 Dresden, Germany.|Interdisciplinary Research Institute, Université des Sciences et des Technologies de Lille (USTL), CNRS USR 3078, 50, Avenue Halley, 59568 Villeneuve d'Ascq, France.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Florescu
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Krull
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Anna H
authors.initials: AH
authors.lastname: Klemm
authors.affiliation: Department of Physics, Faculty of Science, University of Zagreb, Bijenička cesta 32, 10000 Zagreb, Croatia.
authors.forename: Nenad
authors.initials: N
authors.lastname: Pavin
authors.affiliation: Max Planck Institute for the Physics of Complex Systems, Nöthnitzerstr. 38, 01187 Dresden, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Jülicher
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.|Division of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia.
authors.forename: Iva M
authors.initials: IM
authors.lastname: Tolić
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D003198:Computer Simulation; D018386:Kinetochores / Q000378:metabolism*; D008540:Meiosis; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors; D059008:Time-Lapse Imaging