title: The transcriptome of mouse central nervous system myelin.
abstract: Rapid nerve conduction in the CNS is facilitated by insulation of axons with myelin, a specialized oligodendroglial compartment distant from the cell body. Myelin is turned over and adapted throughout life; however, the molecular and cellular basis of myelin dynamics remains elusive. Here we performed a comprehensive transcriptome analysis (RNA-seq) of myelin biochemically purified from mouse brains at various ages and find a surprisingly large pool of transcripts enriched in myelin. Further computational analysis showed that the myelin transcriptome is closely related to the myelin proteome but clearly distinct from the transcriptomes of oligodendrocytes and brain tissues, suggesting a highly selective incorporation of mRNAs into the myelin compartment. The mRNA-pool in myelin displays maturation-dependent dynamic changes of composition, abundance, and functional associations; however ageing-dependent changes after 6 months were minor. We suggest that this transcript pool enables myelin turnover and the local adaptation of individual pre-existing myelin sheaths.
authors.affiliation: Institute of Molecular Biology (IMB), 55128 Mainz, Germany.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Thakurela
authors.affiliation: Institute of Molecular Biology (IMB), 55128 Mainz, Germany.
authors.forename: Angela
authors.initials: A
authors.lastname: Garding
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.
authors.forename: Ramona B
authors.initials: RB
authors.lastname: Jung
authors.affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg-University, 55128 Mainz, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Müller
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Goebbels
authors.affiliation: Institute of Physiology, University Medical Center of the Johannes Gutenberg-University, 55128 Mainz, Germany.
authors.forename: Robin
authors.initials: R
authors.lastname: White
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.
authors.forename: Hauke B
authors.initials: HB
authors.lastname: Werner
authors.affiliation: Institute of Molecular Biology (IMB), 55128 Mainz, Germany.
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Tiwari
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000378:metabolism*; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger