title: Improving the synthesis of phenolic polymer using Coprinus cinereus peroxidase mutant Phe230Ala.
abstract: The F230A mutant of Coprinus cinereus peroxidase (CiP), which has a high stability against radical-inactivation, was previously reported. In the present study, the radical-robust F230A mutant was applied to the oxidative polymerization of phenol. The F230A mutant exhibited better polymerization activities than the wild-type CiP in the presence of water-miscible alcohols i.e., methanol, ethanol, and isopropanol despite its lower stability against alcohols. In particular, the F230A mutant showed a higher consumption of phenol (40%) and yielded phenolic polymer of larger molecular weight (8850Da) in a 50% (v/v) isopropanol-buffer mixture compared with the wild-type CiP (2% and 1519Da, respectively). In addition, the wild-type CiP and F230A mutant had no significant differences in enzyme inactivation by physical adsorption on the polymeric products or by heat incubation, and showed comparable kinetic parameters. These results indicate that high radical stability of the F230A mutant and improved solubility of phenolic polymers in alcohol-water cosolvent systems may synergistically contribute to the production of the high molecular weight phenolic polymer.
authors.affiliation: Interdisciplinary Program of Bioengineering, School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea; Center for Bio-Based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Center for Bio-Based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea.
authors.forename: Jeong Chan
authors.initials: JC
authors.lastname: Joo
authors.affiliation: Center for Bio-Based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea.
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Song
authors.affiliation: Interdisciplinary Program of Bioengineering, School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: yjyoo@snu.ac.kr.
authors.forename: Young Je
authors.initials: YJ
authors.lastname: Yoo
authors.affiliation: Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Republic of Korea. Electronic address: metalkim@kw.ac.kr.
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Kim
mesh_terms: D019943:Amino Acid Substitution; D055162:Biocatalysis; D003302:Coprinus / Q000201:enzymology* / Q000235:genetics*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009195:Peroxidase / Q000235:genetics* / Q000378:metabolism*; D059808:Polyphenols / Q000096:biosynthesis* / Q000737:chemistry; D015202:Protein Engineering; D012997:Solvents
chemical_list: D005656:Fungal Proteins; D059808:Polyphenols; D012997:Solvents; D009195:Peroxidase
keywords: Coprinus cinereus; Enzymatic polymerization; Peroxidase; Phenol; Radical-robust mutant