title: Electrochemical behavior of an anticancer drug 5-fluorouracil at methylene blue modified carbon paste electrode.
abstract: A novel sensor for the determination of 5-fluorouracil was constructed by electrochemical deposition of methylene blue on surface of carbon paste electrode. The electrode surface morphology was studied using Atomic force microscopy and XRD. The electrochemical activity of modified electrode was characterized using cyclic voltammetry and differential pulse method. The developed sensor shows impressive enlargement in sensitivity of 5-fluorouracil determination. The peak currents obtained from differential pulse voltammetry was linear with concentration of 5-fluorouracil in the range 4×10(-5)-1×10(-7)M and detection limit and quantification limit were calculated to be 2.04nM and 6.18nM respectively. Further, the sensor was successfully applied in pharmaceutical and biological fluid sample analysis.
authors.affiliation: Department of Chemistry, K.L.E. Institute of Technology, Gokul, Hubli, affiliated to Visvesvaraya Technological University, 580030, Karnataka, India.
authors.forename: Shikandar D
authors.initials: SD
authors.lastname: Bukkitgar
authors.affiliation: Department of Chemistry, K.L.E. Institute of Technology, Gokul, Hubli, affiliated to Visvesvaraya Technological University, 580030, Karnataka, India. Electronic address: dr.npshetti@gmail.com.
authors.forename: Nagaraj P
authors.initials: NP
authors.lastname: Shetti
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis* / Q000652:urine; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D005472:Fluorouracil / Q000032:analysis* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008751:Methylene Blue / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D002244:Carbon; D008751:Methylene Blue; D005472:Fluorouracil
keywords: 5-Fluorouracil; Electrochemical behavior; Methylene blue modified electrode; Reaction mechanism