title: Ultrasonically extracted β-d-glucan from artificially cultivated mushroom, characteristic properties and antioxidant activity.
abstract: Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers has attracted the attention of the local market. The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity. Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma. The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
authors.affiliation: Manufacturing and Industrial Processes Research Division, The University of Nottingham Malaysia Campus, Faculty of Engineering, Jalan Broga, 43500 Semenyih, Selangor D.E., Malaysia.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Alzorqi
authors.affiliation: Manufacturing and Industrial Processes Research Division, The University of Nottingham Malaysia Campus, Faculty of Engineering, Jalan Broga, 43500 Semenyih, Selangor D.E., Malaysia.
authors.forename: Surya
authors.initials: S
authors.lastname: Sudheer
authors.affiliation: Graduate Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Ting-Jang
authors.initials: TJ
authors.lastname: Lu
authors.affiliation: Manufacturing and Industrial Processes Research Division, The University of Nottingham Malaysia Campus, Faculty of Engineering, Jalan Broga, 43500 Semenyih, Selangor D.E., Malaysia. Electronic address: Sivakumar.Manickam@nottingham.edu.my.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Manickam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000737:chemistry; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D000069453:Ultrasonic Waves
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005936:Glucans; D009005:Monosaccharides; D010636:Phenols; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Extraction; Ganoderma; Polysaccharides; Ultrasound; β-d-Glucans