title: The endochitinase ChiA Btt of Bacillus thuringiensis subsp. tenebrionis DSM-2803 and its potential use to control the phytopathogen Colletotrichum gloeosporioides.
abstract: Bacillus thuringiensis subsp. tenebrionis DSM-2803 has been studied extensively and spore/crystal mixtures of this strain are used widely in commercial products to control coleopteran pests. The endochitinase chiA Btt gene of B. thuringiensis subsp. tenebrionis DSM-2803 was cloned and expressed in Escherichia coli. The recombinant 6x-histidine tagged protein (rChiA Btt, ~74 kDa), was purified by a HiTrap Ni affinity column. The Kmof rChiA Btt was 0.847 μmol L-1and its optimal activity occurred at pH 7 and ~40°C. Most divalent cations reduced endochitinase activity but only Hg+2abolished activity of the enzyme. We report for the first time the characterization of a chitinase synthesized by B. thuringiensis subsp. tenebrionis DSM-2803, and show that the purified rChiA74 Btt reduced the radial growth and increased the hyphal density of Colletotrichium gloeosporioides, the etiological agent of "anthracnose" in plants.
authors.affiliation: Aniversidad Autónoma de Coahuila, Escuela de Ciencias Biológicas, Torreón, Coahuila, 27104, México.|Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México.
authors.forename: Norma M
authors.initials: NM
authors.lastname: de la Fuente-Salcido
authors.affiliation: Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México.|Departamento de Alimentos, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México.
authors.forename: Luz E
authors.initials: LE
authors.lastname: Casados-Vázquez
authors.affiliation: Aniversidad Autónoma de Coahuila, Escuela de Ciencias Biológicas, Torreón, Coahuila, 27104, México.
authors.forename: Ada P
authors.initials: AP
authors.lastname: García-Pérez
authors.affiliation: Tecnológico de Monterrey Campus Querétaro, Epigmenio González 500 Fracc, San Pablo, Querétaro, Qro, 76130, México.
authors.forename: Uriel E
authors.initials: UE
authors.lastname: Barboza-Pérez
authors.affiliation: Department of Natural and Mathematical Sciences, California Baptist University, 8432 Magnolia Avenue, Riverside, 92504, California.|Department of Entomology, University of California, Riverside, California, 92521.
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Bideshi
authors.affiliation: Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México.|Departamento de Alimentos, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México.
authors.forename: Rubén
authors.initials: R
authors.lastname: Salcedo-Hernández
authors.affiliation: Universidad Autónoma de Querétaro. DIPA, PROPAC, Facultad de Química, Querétaro, Qro, 76010, México.
authors.forename: Blanca E
authors.initials: BE
authors.lastname: García-Almendarez
authors.affiliation: Posgrado en Biociencias, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México. josebar@ugto.mx.|Departamento de Alimentos, División de Ciencias de la Vida, Universidad de Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato, 36500, México. josebar@ugto.mx.
authors.forename: José E
authors.initials: JE
authors.lastname: Barboza-Corona
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D001413:Bacillus thuringiensis / Q000201:enzymology*; D061046:Biological Control Agents; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D020231:Colletotrichum / Q000187:drug effects*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D061046:Biological Control Agents; D011994:Recombinant Proteins; D002688:Chitinases
keywords: 
  Colletotrichium gloeosporioides
  ; Bacillus thuringiensis subsp. tenebrionis; ChiA Btt; DSM-2803; endochitinase; phytopathogen