title: Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules.
abstract: The present investigation evaluated the ability of an experimental di-calcium phosphate (DCP) desensitising agent used alone or combined with phytosphingosine (PHS) to occlude dentine tubules and resist a citric acid (CA) or artificial saliva (AS) challenge. Three groups of human dentine specimens (DS) were treated with the following: (1) PHS alone, (2) DCP or (3) a combination of PHS and DCP. Dentine hydraulic conductance of DS was evaluated using a digital flow sensor at 6.9 kPa. The average fluid volume for each of the treated DS was used to calculate the total dentine permeability reduction (%P) prior to and following CA immersion for 1 min or AS immersion for 4 weeks. The treated DS were subjected to both scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy analysis. Statistically significant differences (%P) were identified between the groups by ANOVA and Fisher's multiple comparison test (p < 0.05), respectively. Interestingly, both PHS and DCP appeared to work synergistically. DS treated with DCP or PHS/DCP demonstrated a significant reduction (%P) prior to and following CA or AS challenge (p < 0.05). Both the SEM and FTIR analyses showed consistent brushite crystals occluding the dentine tubules. Conversely, the application of PHS alone failed to demonstrate any significant reduction of dentine permeability (p > 0.05) or show any evidence of occlusion of the dentine tubules. DCP can be used alone or combined with PHS to decrease the dentine permeability as well as to resist a CA and AS challenge. These results would, therefore, suggest that DCP may be a suitable treatment option for dentine hypersensitivity.
authors.affiliation: Departamento de Odontologx00ED;a, Facultad de Ciencias de la Salud, Universidad CEU-Cardenal Herrera, Valencia, Spain.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sauro
authors.forename: Ching-Yang
authors.initials: CY
authors.lastname: Lin
authors.forename: Floris J
authors.initials: FJ
authors.lastname: Bikker
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cama
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.forename: José M
authors.initials: JM
authors.lastname: Soria
authors.forename: Alessia
authors.initials: A
authors.lastname: D''Onofrio
authors.forename: David
authors.initials: D
authors.lastname: Gillam
mesh_terms: D000704:Analysis of Variance; D002130:Calcium Phosphates / Q000494:pharmacology* / Q000627:therapeutic use; D019343:Citric Acid / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003806:Dentin Permeability / Q000187:drug effects; D003807:Dentin Sensitivity / Q000188:drug therapy*; D004357:Drug Synergism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012464:Saliva, Artificial / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D002130:Calcium Phosphates; D057133:Dentin Desensitizing Agents; D012464:Saliva, Artificial; D019343:Citric Acid; C012491:phytosphingosine; C485829:calcium phosphate, dibasic, anhydrous; D013110:Sphingosine; C494366:calcium phosphate, dibasic, dihydrate