title: [Identification and in vitro antifungal susceptibility of vaginal Candida spp. isolates to fluconazole, clotrimazole and nystatin].
abstract: OBJECTIVE
  The aim of this study was to identify and determine the in vitro antifungal susceptibility testing to clotrimazole, fluconazole, and nystatin of 145 clinical isolates of Candida spp.
  
  
  METHODS
  M27-A3 microdilution method was used to determine minimal inhibitory concentrations (MIC) and partial MICs (MIC50 and MIC90) of drugs. A total of 145 isolates were studied, 126 were C. albicans, 16 C. glabrata, 2 C. parapsilosis y 1 C. tropicalis.
  
  
  RESULTS
  MIC50 and MIC90 for FLZ against C. albicans were 0.25 mg/L and 1 mg/L respectively and for C. glabrata was achieved at 8 mg/L and 16 mg/L for fluconazole. Five isolates of C. albicans and one isolate of C. tropicalis were in vitro resistant to fluconazole (M27-S4). In C. albicans MIC50 and MIC90 for clotrimazole were of 0.03 mg/L and 0.06 mg/L, respectively. These values for C. glabrata were 0.25 mg/L and 1 mg/L, respectively. Five C. glabrata and 1 C. tropicalis were in vitro resistant to clotrimazole. MIC50 and MIC90 of nystatin were of 1 mg/L and 2 mg/L, respectively for C. albicans and C. glabrata.
  
  
  CONCLUSIONS
  In this study, C. albicans is the most frequently isolated yeast, followed by C. glabrata. The antifungals tested were found to be in vitro active for the isolates, except for 6 isolates for fluconazole and 6 to clotrimazole.
authors.forename: M C
authors.initials: MC
authors.lastname: Diaz
authors.forename: R
authors.initials: R
authors.lastname: Camponovo
authors.forename: I
authors.initials: I
authors.lastname: Araya
authors.forename: A
authors.initials: A
authors.lastname: Cerda
authors.forename: M P
authors.initials: MP
authors.lastname: Santander
authors.affiliation: Alfonso-Javier Carrillo-Muñoz, Dpto. Microbiología-Micología. ACIAM. Barcelona. P.O. Box 10178. E-08080 Barcelona, Spain. acarrillo@aciam.es.
authors.forename: A J
authors.initials: AJ
authors.lastname: Carrillo-Muñoz
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002181:Candidiasis, Vulvovaginal / Q000453:epidemiology / Q000382:microbiology*; D003022:Clotrimazole / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009761:Nystatin / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D009761:Nystatin; D015725:Fluconazole; D003022:Clotrimazole