title: Multiepitope Templates Imprinted Particles for the Simultaneous Capture of Various Target Proteins.
abstract: To achieve the simultaneous capture of various target proteins, the multiepitope templates imprinted particles were developed by phase inversion-based poly(ether sulfone) (PES) self-assembly. Herein, with the top three high-abundance proteins in the human plasma, serum albumin, immunoglobulin G, and transferrin, as the target proteins, their N-terminal peptides were synthesized as the epitope templates. After the preorganization of three epitopes and PES in dimethylacetamide, the multiepitope templates imprinted particles were formed in water through self-assembly, by which the simultaneous recognition of three target proteins in human plasma was achieved with high selectivity. Furthermore, the binding kinetics study proved that the adsorption mechanism in this imprinting system toward three epitope templates was the same as that on the single-epitope imprinting polymer. These results demonstrate that our proposed multiepitope templates imprinting strategy might open a new era of artificial antibodies to achieve the recognition of various targets simultaneously.
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Kaiguang
authors.initials: K
authors.lastname: Yang
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences , Beijing, 100049, China.
authors.forename: Senwu
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences , Beijing, 100049, China.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , 457 Zhongshan Road, Dalian, 116023, China.|University of Chinese Academy of Sciences , Beijing, 100049, China.
authors.forename: Lukuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000939:Epitopes; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007700:Kinetics; D054802:Molecular Imprinting; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D000075462:Serum Albumin, Human / Q000032:analysis*; D013450:Sulfones / Q000138:chemical synthesis / Q000737:chemistry; D014168:Transferrin / Q000032:analysis*
chemical_list: D000939:Epitopes; D007074:Immunoglobulin G; D011108:Polymers; D013450:Sulfones; D014168:Transferrin; C022840:polyether sulfone; D000075462:Serum Albumin, Human