title: Tracking Effector and Memory NK Cells During MCMV Infection.
abstract: In C57BL/6 mice, NK cells expressing the activating receptor Ly49H proliferate robustly in response to mouse cytomegalovirus (MCMV) infection. The expansion of Ly49H(+) NK cells peaks at approximately 1 week post-infection, and is then followed by a distinct contraction phase that ultimately leaves a small but long-lived pool of MCMV-experienced Ly49H(+) NK cells that are capable of mediating enhanced memory-like responses during subsequent encounters with MCMV. Here we describe an adoptive transfer model in which the expansion, contraction, and memory cell persistence of transferred Ly49H(+) NK cells are tracked in congenic C57BL/6 hosts following MCMV infection.
authors.affiliation: Department of Microbiology, Biochemistry and Moleculor Genetics, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, New York, NJ, 07103, USA.
authors.forename: Aimee M
authors.initials: AM
authors.lastname: Beaulieu
authors.affiliation: Immunology Department, Memorial Sloan-Kettering Cancer Center, 408 East 69th Street, ZRC-1402, New York, NY, 10065, USA. sunj@mskcc.org.
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Sun
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000378:metabolism; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology / Q000637:transplantation*; D051379:Mice; D008810:Mice, Inbred C57BL; D018146:Muromegalovirus / Q000276:immunology; D055650:NK Cell Lectin-Like Receptor Subfamily A / Q000378:metabolism
chemical_list: C527096:Klra8 protein, mouse; D055650:NK Cell Lectin-Like Receptor Subfamily A
keywords: Innate lymphocytes; MCMV; Memory; NK cells