title: Food restriction attenuates oxidative stress in brown adipose tissue of striped hamsters acclimated to a warm temperature.
abstract: It has been suggested that the up-regulation of uncoupling proteins (UCPs) decreases reactive oxygen species (ROS) production, in which case there should be a negative relationship between UCPs expression and ROS levels. In this study, the effects of temperature and food restriction on ROS levels and metabolic rate, UCP1 mRNA expression and antioxidant levels were examined in the brown adipose tissue (BAT) of the striped hamsters (Cricetulus barabensis). The metabolic rate and food intake of hamsters which had been restricted to 80% of ad libitum food intake, and acclimated to a warm temperature (30°C), decreased significantly compared to a control group. Hydrogen peroxide (H2O2) levels were 42.9% lower in food restricted hamsters than in the control. Malonadialdehyde (MDA) levels of hamsters acclimated to 30°C that were fed ad libitum were significantly higher than those of the control group, but 60.1% lower than hamsters that had been acclimated to the same temperature but subject to food restriction. There were significantly positive correlations between H2O2 and, MDA levels, catalase activity, and total antioxidant capacity. Cytochrome c oxidase activity and UCP1 mRNA expression significantly decreased in food restricted hamsters compared to the control. These results suggest that warmer temperatures increase oxidative stress in BAT by causing the down-regulation of UCP1 expression and decreased antioxidant activity, but food restriction may attenuate the effects.
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang 325035, China.
authors.forename: Ji-Ying
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang 325035, China.
authors.forename: Xiao-Ya
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: School of Agricultural Science, Liaocheng University, Liaocheng, Shandong 252059, China.
authors.forename: Gui-Ying
authors.initials: GY
authors.lastname: Wang
authors.affiliation: School of Agricultural Science, Liaocheng University, Liaocheng, Shandong 252059, China.
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Wang
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang 325035, China. Electronic address: zhao73@foxmail.com.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Zhao
mesh_terms: D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000502:physiology*; D000818:Animals; D001481:Basal Metabolism; D002374:Catalase / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus / Q000502:physiology*; D015536:Down-Regulation; D004435:Eating; D003576:Electron Transport Complex IV / Q000378:metabolism; D005215:Fasting / Q000502:physiology*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D000071256:Uncoupling Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000071256:Uncoupling Protein 1; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D003576:Electron Transport Complex IV
keywords: Antioxidants; Brown adipose tissue (BAT); Food restriction; Oxidative stress; Striped hamster; Temperature; Uncoupling protein