title: Assembling Multi-subunit Complexes Using Mammalian Expression.
abstract: In this chapter conventional and emerging new technologies for the production of complex biologics in mammalian expression systems are summarized. The essential features of the most relevant methods to generate stable production cell lines for the expression of recombinant multi-protein complexes are described. Especially the promising multiple targeted integration strategy by Flp or CRISPR/Cas9 mediated recombination and their future impact on multi-protein expression are highlighted.
authors.affiliation: Department of Structure and Function of Proteins, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Bahar
authors.initials: B
authors.lastname: Baser
authors.affiliation: Department of Structure and Function of Proteins, Helmholtz Centre for Infection Research, Braunschweig, Germany. joop.vandenheuvel@helmholtz-hzi.de.
authors.forename: Joop
authors.initials: J
authors.lastname: van den Heuvel
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D018929:Cell Culture Techniques; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D046912:Multiprotein Complexes; D015202:Protein Engineering / Q000379:methods*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D011994:Recombinant Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase
keywords: Flp/FRT recombination; Multi RMCE; MultiMam; Stable cell line; Targeted integration