title: Antioxidant and Myocardial Preservation Activities of Natural Phytochemicals from Mung Bean (Vigna radiata L.) Seeds.
abstract: Mung bean (Vigna radiata L.) seeds (MBS) contain abundant nutrients with biological activities. This study was aimed to isolate key bioactive components from MBS with antioxidant and myocardial preservation activities. A new flavonoid C-glycoside, isovitexin-6″-O-α-l-glucoside, and 14 known compounds were obtained. Their structures were identified by extensive 1D and 2D NMR and FT-ICR-MS spectroscopic analyses. The antioxidant activities of these compounds were evaluated. Compounds 1-5 and 7-10 displayed 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(•+)) scavenging activity, but only 5 and 7 exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH(•)) scavenging activity. The myocardial preservation effect of 2, 3, and MBS were investigated by measuring the serum levels of LDH, CK, and AST as well as the tissue level of MDA and SOD. The results demonstrated that 2, 3, and MBS had a significant protective effect against ISO-induced myocardial ischemia. MBS can be regarded as a potential new source of antioxidants and myocardial preservation agents.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, China. zhiguo-yu@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, China. zhiguo-yu@163.com
authors.forename: Jiawei
authors.initials: J
authors.lastname: Chang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, China. zhiguo-yu@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, China. zhiguo-yu@163.com
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Dalian Weida Pharmacy Co., Limited , 12-7 Zhuhai Street, Xigang District, Dalian 116011, China.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, China. zhiguo-yu@163.com
authors.forename: Yunli
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, China. zhiguo-yu@163.com
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy / Q000517:prevention & control*; D064209:Phytochemicals / Q000008:administration & dosage* / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D000070658:Vigna / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts
keywords: antioxidant; functional foods; isovitexin; mung bean seeds; myocardial preservation; vitexin