title: The wiring diagram of a glomerular olfactory system.
abstract: The sense of smell enables animals to react to long-distance cues according to learned and innate valences. Here, we have mapped with electron microscopy the complete wiring diagram of the Drosophila larval antennal lobe, an olfactory neuropil similar to the vertebrate olfactory bulb. We found a canonical circuit with uniglomerular projection neurons (uPNs) relaying gain-controlled ORN activity to the mushroom body and the lateral horn. A second, parallel circuit with multiglomerular projection neurons (mPNs) and hierarchically connected local neurons (LNs) selectively integrates multiple ORN signals already at the first synapse. LN-LN synaptic connections putatively implement a bistable gain control mechanism that either computes odor saliency through panglomerular inhibition, or allows some glomeruli to respond to faint aversive odors in the presence of strong appetitive odors. This complete wiring diagram will support experimental and theoretical studies towards bridging the gap between circuits and behavior.
authors.affiliation: Department of Physics, Harvard University, Cambridge, United States.|Center for Brain Science, Harvard University, Cambridge, United States.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Berck
authors.affiliation: EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.|Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Avinash
authors.initials: A
authors.lastname: Khandelwal
authors.affiliation: Department of Physics, Harvard University, Cambridge, United States.|Center for Brain Science, Harvard University, Cambridge, United States.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Claus
authors.affiliation: Department of Physics, Harvard University, Cambridge, United States.|Center for Brain Science, Harvard University, Cambridge, United States.
authors.forename: Luis
authors.initials: L
authors.lastname: Hernandez-Nunez
authors.affiliation: Department of Physics, Harvard University, Cambridge, United States.|Center for Brain Science, Harvard University, Cambridge, United States.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Si
authors.affiliation: Department of Physics, Harvard University, Cambridge, United States.
authors.forename: Christopher J
authors.identifier: 0000-0001-8746-0680
authors.initials: CJ
authors.lastname: Tabone
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: James W
authors.initials: JW
authors.lastname: Truman
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Rick D
authors.initials: RD
authors.lastname: Fetter
authors.affiliation: EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.|Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Matthieu
authors.identifier: 0000-0002-2267-0262
authors.initials: M
authors.lastname: Louis
authors.affiliation: Department of Physics, Harvard University, Cambridge, United States.|Center for Brain Science, Harvard University, Cambridge, United States.
authors.forename: Aravinthan Dt
authors.initials: AD
authors.lastname: Samuel
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
authors.forename: Albert
authors.identifier: 0000-0003-4941-6536
authors.initials: A
authors.lastname: Cardona
mesh_terms: D000818:Animals; D004330:Drosophila / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009434:Neural Pathways / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D066194:Olfactory Cortex / Q000648:ultrastructure
keywords: D. melanogaster; Drosophila; connectomics; electron microscopy; neural circuits; neuroscience; olfaction