title: Loss of nitrate reductases NIA1 and NIA2 impairs stomatal closure by altering genes of core ABA signaling components in Arabidopsis.
abstract: Nitrate reductases NIA1 and NIA2 determine NO production in plants and are critical to abscisic acid (ABA)-induced stomatal closure. However, the role for NIA1 and NIA2 in ABA signaling has not been paid much attention in nitrate reductase loss-of-function mutant nia1nia2. Recently, we have demonstrated that ABA-inhibited K(+)in current and ABA-enhanced slow anion current were absent in nia1nia2. Exogenous NO restored regulation of these channels for stomatal closure in nia1nia2. In this study, we found that mutating NIA1 and NIA2 impaired nearly all the key components of guard cell ABA signaling pathway in Arabidopsis. We also propose a simplified model for ABA signaling in the nia1nia2 mutant.
authors.affiliation: a School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University , Penrith , Australia.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: a School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University , Penrith , Australia.
authors.forename: Shengguan
authors.initials: S
authors.lastname: Cai
authors.affiliation: b Laboratory of Plant Physiology and Biophysics, Bower Building, University of Glasgow , Glasgow , United Kingdom.
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University , Penrith , Australia.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D050901:Nitrate Reductase / Q000235:genetics* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D054046:Plant Stomata / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D009569:Nitric Oxide; D000040:Abscisic Acid; C000620946:NIA1 protein, Arabidopsis; C000620947:NIA2 protein, Arabidopsis; D050901:Nitrate Reductase
keywords: Abscisic acid; gene expression; guard cell; nitric oxide; signaling transduction