title: Effects of experimental left varicocele repair on hypoxia-inducible factor-1α and vascular endothelial growth factor expressions and angiogenesis in rat testis.
abstract: This study was aimed to investigate the effects of experimental left varicocele (ELV) repair on hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expressions and angiogenesis in rat testis. ELVs were surgically created in 26 adult male Sprague-Dawley rats. Thirty days after surgery, ELV repair was performed in 13 of the rats. All rats subsequently underwent orchiectomy 30 days after the last laparotomy. Histology of ELV-repaired testicles was compared to that of the unrepaired (ELV) group. The frequency of positive HIF-1α findings was significantly lower in the ELV-repaired than in the ELV group. The frequency of positive VEGF findings was also lower in the ELV-repaired than in the ELV group, although the difference was not statistically significant (P = 0.238). The mean microvessel density in ELV-repair group was significantly lower than that in the ELV group (P = 0.002). Our study demonstrated that ELV repair may protect tissues from hypoxia and hypoxia-related pathophysiologic events, such as angiogenesis, in rat testis.
authors.affiliation: Department of Urology, Baskent University Adana Medical and Research Center, Adana, Turkey.
authors.forename: M R
authors.initials: MR
authors.lastname: Goren
authors.affiliation: Department of Urology, Baskent University Adana Medical and Research Center, Adana, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Kilinc
authors.affiliation: Department of Pathology, Baskent University Adana Medical and Research Center, Adana, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Kayaselcuk
authors.affiliation: Department of Urology, Baskent University Adana Medical and Research Center, Adana, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Ozer
authors.affiliation: Department of Urology, Baskent University School of Medicine, Ankara, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Oguzulgen
authors.affiliation: Department of Urology, Baskent University School of Medicine, Ankara, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Hasirci
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D000860:Hypoxia / Q000150:complications; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008297:Male; D009389:Neovascularization, Pathologic / Q000209:etiology / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D014646:Varicocele / Q000473:pathology / Q000601:surgery*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat
keywords: HIF-1α protein; hypoxia; varicocele; varicocelectomy; vascular endothelial growth factor