title: A Belgian Serosurveillance/Seroprevalence Study of Diphtheria, Tetanus and Pertussis Using a Luminex xMAP Technology-Based Pentaplex.
abstract: Serosurveillance and seroprevalence studies are an essential tool to monitor vaccine-preventable diseases. We have developed a magnetic bead-based pentaplex immunoassay (MIA) for the simultaneous detection of IgG antibodies against diphtheria toxin (DT), tetanus toxin (TT), pertussis toxin (PT), filamentous hemagglutinin (FHA) and pertactin (Prn). The in-house pentaplex MIA showed a good correlation with commercial ELISAs with correlation coefficients between 0.89 for PT and 0.98 for TT. Intra- and inter-assay variability was <10%. A total of 670 anonymized serum samples collected in 2012 in Belgian adults (ages 20-29.9 years) were analyzed. Geometric mean concentrations (GMC) were 0.2 (0.13-0.29) IU/mL for DT, 0.63 (0.45-0.82) IU/mL for TT, 3.9 (2.6-5.8) IU/mL for PT, 16.3 (11.7-22.7) IU/mL for FHA and 15.4 (10.1-23.6) IU/mL for Prn. Antibody concentrations were below the protective level of 0.1 IU/mL in 26.4% of the sera for DT and in 8.6% of the sera for TT. Anti-PT IgG concentrations indicative of recent pertussis infection (>125 IU/mL) were detected in 1.2% of the subjects. High anti-PT antibodies were not correlated with high antibodies against any of the four other vaccine antigens. This pentaplex MIA will be used for a new large-scale Belgian serosurveillance/seroprevalence study of diphtheria, tetanus and pertussis.
authors.affiliation: National Reference Centre <i>Bordetella</i> and <i>Toxigenic Corynebacteria</i>, Service Immunology, Scientific Institute of Public Health (WIV-ISP, Site Ukkel), 1180 Brussels, Belgium. RaissaNadege.CABORE@wiv-isp.be.
authors.forename: Raissa Nadège
authors.initials: RN
authors.lastname: Caboré
authors.affiliation: National Reference Centre <i>Bordetella</i> and <i>Toxigenic Corynebacteria</i>, University Hospital VUB, UZ Brussels, 1090 Jette, Belgium. Denis.Pierard@uzbrussel.be.
authors.forename: Denis
authors.initials: D
authors.lastname: Piérard
authors.affiliation: National Reference Centre <i>Bordetella</i> and <i>Toxigenic Corynebacteria</i>, Service Immunology, Scientific Institute of Public Health (WIV-ISP, Site Ukkel), 1180 Brussels, Belgium. kris.huygen@wiv-isp.be.
authors.forename: Kris
authors.initials: K
authors.lastname: Huygen
keywords: diphtheria; multiplex; pertussis; seroprevalence; serosurveillance; tetanus