title: Antinociceptive, antioxidant and phytochemical studies of Pakistani medicinal plants.
abstract: The aim of the current study was to evaluate the antinociceptive activity of the selected Pakistani medicinal plants (Chenopodium botrys, Micromeria biflora and Teucrium stocksianum) in-vivo followed by their antioxidant potential against 1,1-diphenyl-2-picrylhidrazyl (DPPH) in-vitro. The results demonstrated profound antinociceptive effect of both the crude methanolic extract of Chenopodium botrys (CBM) and subsequent aqueous fraction (CBW) of C. botrys with 80.76% and 84% pain relief in acetic acid induced writhing test at 100 mg/kg i.p respectively. Similarly the crude methanolic extract of Micromeria biflora (MBM) and its subsequent aqueous fraction (MBW) with 66.46% 78.08% pain reversal in acetic acid induced writhing test respectively at 100mg/kg i.p. However, the crude methanolic extract and isolated water fraction of Teucrium stocksianum (TS) did not show any significant effect at test doses. Both the crude extracts and aqueous fractions of selected medicinal plants exhibited marked scavenging effects on DPPH and therefore strongly support the antinociceptive activity. Phytochemical analysis indicated the presence of various classes of natural products (alkaloids, terpenoids, flavonoids etc.) and thus the current finding can be attributed to the presence of these compounds. In short, our findings provide a strong scientific background to the folk uses C. botrys and M. biflora in the management of various painful conditions.
authors.affiliation: Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, Pakistan.
authors.forename: Ghias
authors.initials: G
authors.lastname: Uddin
authors.affiliation: Department of Geology, University of Swabi, Anbar, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.affiliation: H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan.
authors.forename: Bina Shaheen
authors.initials: BS
authors.lastname: Siddiqui
authors.affiliation: Department of Pharmacy, Abdul Wali Khan University Mardan, Pakistan.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.affiliation: Department of Botany Islamia College University, Peshawar, Pakistan.
authors.lastname: Barkatullah
authors.affiliation: Department of Botany Islamia College University, Peshawar, Pakistan.
authors.forename: Rooh
authors.initials: R
authors.lastname: Ullah
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D027462:Chenopodium / Q000737:chemistry*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D019686:Lamiaceae / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D008807:Mice, Inbred BALB C; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control*; D010154:Pakistan; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D031367:Teucrium / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000700:Analgesics; D000975:Antioxidants; D001713:Biphenyl Compounds; D064209:Phytochemicals; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; C040618:teucrium extract; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl; D019342:Acetic Acid; D000432:Methanol