title: Influence of iron on modulation of the antioxidant system in rat brains exposed to lead.
abstract: The objective of this study was to evaluate markers of oxidative stress in the brains of rats exposed to lead acetate (Pb(C2H3O2)2), either associated or not associated with ferrous sulfate (FeSO4). A total of 36 weaning rats (Rattus norvegicus) were divided into 6 groups of six animals and exposed to lead acetate for six weeks. In the control group (control), the animals received deionized water. The Pb260 and Pb260 + Fe received 260 µM lead acetate, and the Pb1050 and Pb1050 + Fe received 1050 µM lead acetate. The Pb260 + Fe and Pb1050 + Fe were supplemented with 20 mg of ferrous sulfate/Kg body weight every 2 days. Group Fe received deionized water and ferrous sulfate. The rat brains were collected to analyze the enzymatic activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and the concentration of reduced glutathione (GSH), lipid peroxidation (TBARS), and total antioxidant substance (TAS) (DPPH•technique). The activity of SOD and GPx in the experimental groups decreased compared to the control, together with the concentration of GSH (p < 0.05). For CAT analysis, SOD tended to increase in concentration in the experimental groups without a concomitant exposure to FeSO4, whereas GPx showed a slight tendency to increase in activity compared to the control. For TAS-DPPH•, there was a decrease in the experimental groups (p < 0.05). According to the results, SOD, GPx, and GSH were affected by lead acetate and exposure to ferrous sulfate changed this dynamic. However, further studies are needed to verify whether ferrous sulfate acts as a protectant against the toxic effects of lead. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 813-822, 2017.
authors.affiliation: Department of Biological Sciences/Biochemistry, Bauru School of Dentistry - University of São Paulo. Alameda Dr. Octavio Pinheiro Brisolla, 9-75 Bauru-SP, 17012-901, Brazil.
authors.forename: Maria Cecília de Freitas
authors.initials: MC
authors.lastname: Ferreira
authors.affiliation: Department of Biological Sciences/Biochemistry, Bauru School of Dentistry - University of São Paulo. Alameda Dr. Octavio Pinheiro Brisolla, 9-75 Bauru-SP, 17012-901, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Zucki
authors.affiliation: Department of Biological Sciences/Biochemistry, Bauru School of Dentistry - University of São Paulo. Alameda Dr. Octavio Pinheiro Brisolla, 9-75 Bauru-SP, 17012-901, Brazil.
authors.forename: Josilene Luciene
authors.initials: JL
authors.lastname: Duarte
authors.affiliation: Department of Biological Sciences/Biochemistry, Bauru School of Dentistry - University of São Paulo. Alameda Dr. Octavio Pinheiro Brisolla, 9-75 Bauru-SP, 17012-901, Brazil.
authors.forename: Flávia Godoy
authors.initials: FG
authors.lastname: Iano
authors.affiliation: Department of Chemistry, Faculty of Sciences, São Paulo State University, Av. Engenheiro Luiz Edmundo Carrijo Coube, 1401 Bauru-SP 17033-360, Brazil.
authors.forename: Valdecir Farias
authors.initials: VF
authors.lastname: Ximenes
authors.affiliation: Department of Biological Sciences/Biochemistry, Bauru School of Dentistry - University of São Paulo. Alameda Dr. Octavio Pinheiro Brisolla, 9-75 Bauru-SP, 17012-901, Brazil.
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
authors.affiliation: Department of Biological Sciences/Biochemistry, Bauru School of Dentistry - University of São Paulo. Alameda Dr. Octavio Pinheiro Brisolla, 9-75 Bauru-SP, 17012-901, Brazil.
authors.forename: Rodrigo Cardoso de
authors.initials: RC
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005296:Ferrous Compounds / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009942:Organometallic Compounds / Q000032:analysis / Q000097:blood / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005296:Ferrous Compounds; D009942:Organometallic Compounds; C020748:ferrous sulfate; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; C008261:lead acetate
keywords: antioxidants; brain; catalase; ferrous sulfate; lead