title: Long non-coding RNA ADNCR suppresses adipogenic differentiation by targeting miR-204.
abstract: Adipogenesis is a complex and precisely orchestrated process mediated by a network of adipogenic regulatory factors. Several studies have highlighted the relevance of lncRNAs in adipocyte differentiation, but the precise molecular mechanism has largely remained elusive. In the present study, we performed Ribo-Zero RNA-Seq to investigate both the poly(A)+and poly(A)-lncRNAs of in vitro cultured bovine preadipocytes and differentiated adipocytes. A stringent set of 2882 lncRNAs was finally identified. A comparison of the lncRNAs expression profiles revealed that 16 lncRNAs are differentially expressed during adipocyte differentiation. We focused on the most downregulated lncRNA, which we named adipocyte differentiation-associated long noncoding RNA (ADNCR). Mechanistically, ADNCR inhibited adipocyte differentiation by functioning as a competing endogenous RNA (ceRNA) for miR-204, thereby augmenting the expression of the miR-204 target gene, SIRT1, which is known to inhibit adipocyte differentiation and adipogenic gene expression by docking with NCoR and SMART to repress PPARγ activity. Our data not only provide a valuable genomic resource for the identification of lncRNAs with functional roles in adipocyte differentiation but also reveal new insights into understanding the mechanisms of adipogenic differentiation.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Mingxun
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Hanfang
authors.initials: H
authors.lastname: Cai
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Martin
authors.initials: M
authors.lastname: Plath
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, Animal Genomics & Improvement Laboratory, Beltsville, MD, United States.
authors.forename: Congjun
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.affiliation: Bureau of Animal Husbandry of Biyang County, Biyang, Henan 463700, China.
authors.forename: Fengpeng
authors.initials: F
authors.lastname: Lin
authors.affiliation: Animal Health Supervision in Henan Province, Zhengzhou, Henan 450008, China. Electronic address: postbai@sohu.com.
authors.forename: Yueyu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: chenhong1212@263.net.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015536:Down-Regulation / Q000235:genetics; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D062085:RNA, Long Noncoding / Q000502:physiology*
chemical_list: C574692:MIRN204 microRNA, mouse; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: ADNCR; Adipogenesis; Long noncoding RNAs; Ribo-Zero RNA-Seq; ceRNA