title: Combined toxicity of silica nanoparticles and methylmercury on cardiovascular system in zebrafish (Danio rerio) embryos.
abstract: This study was to investigate the combined toxicity of silica nanoparticles (SiNPs) and methylmercury (MeHg) on cardiovascular system in zebrafish (Danio rerio) embryos. Ultraviolet absorption analysis showed that the co-exposure system had high absorption and stability. The dosages used in this study were based on the NOAEL level. Zebrafish embryos exposed to the co-exposure of SiNPs and MeHg did not show any cardiovascular malformation or atrioventricular block, but had an inhibition effect on bradycardia. Using o-Dianisidine for erythrocyte staining, the cardiac output of zebrafish embryos was decreased gradually in SiNPs, MeHg, co-exposure groups, respectively. Co-exposure of SiNPs and MeHg enhanced the vascular endothelial damage in Tg(fli-1:EGFP) transgenic zebrafish line. Moreover, the co-exposure significantly activated the oxidative stress and inflammatory response in neutrophils-specific Tg(mpo:GFP) transgenic zebrafish line. This study suggested that the combined toxic effects of SiNPs and MeHg on cardiovascular system had more severe toxicity than the single exposure alone.
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China. Electronic address: jcduan@ccmu.edu.cn.
authors.forename: Junchao
authors.initials: J
authors.lastname: Duan
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China.
authors.forename: Hejing
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Chemistry, Renmin University of China, Beijing 100872, PR China. Electronic address: yapeiwang@ruc.edu.cn.
authors.forename: Yapei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China. Electronic address: zwsun@ccmu.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002319:Cardiovascular System / Q000187:drug effects*; D004357:Drug Synergism; D004625:Embryo, Nonmammalian; D004912:Erythrocytes / Q000187:drug effects; D008767:Methylmercury Compounds / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity*; D019055:No-Observed-Adverse-Effect Level; D017382:Reactive Oxygen Species / Q000378:metabolism; D012822:Silicon Dioxide / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D008767:Methylmercury Compounds; D017382:Reactive Oxygen Species; D012822:Silicon Dioxide
keywords: Cardiovascular system; Combined toxicity; Methylmercury; Silica nanoparticles; Zebrafish embryos