title: Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm.
abstract: Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post-thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250-μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm. In addition, higher post-thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen-thawed process.
authors.affiliation: Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran.
authors.forename: M S
authors.initials: MS
authors.lastname: Aramli
authors.affiliation: Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Golshahi
authors.affiliation: Rajaee Sturgeon Propagation Center, Sari, Mazandaran, Iran.
authors.forename: R M
authors.initials: RM
authors.lastname: Nazari
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Golpour
authors.affiliation: Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Aramli
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005399:Fishes / Q000502:physiology*; D005973:Glutamine / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D005973:Glutamine