title: Coronin 1C-free primary mouse fibroblasts exhibit robust rearrangements in the orientation of actin filaments, microtubules and intermediate filaments.
abstract: Coronin 1C is an established modulator of actin cytoskeleton dynamics. It has been shown to be involved in protrusion formation, cell migration and invasion. Here, we report the generation of primary fibroblasts from coronin 1C knock-out mice in order to investigate the impact of the loss of coronin 1C on cellular structural organisation. We demonstrate that the lack of coronin 1C not only affects the actin system, but also the microtubule and the vimentin intermediate filament networks. In particular, we show that the knock-out cells exhibit a reduced proliferation rate, impaired cell migration and protrusion formation as well as an aberrant subcellular localisation and function of mitochondria. Moreover, we demonstrate that coronin 1C specifically interacts with the non-α-helical amino-terminal domain ("head") of vimentin. Our data suggest that coronin 1C acts as a cytoskeletal integrator of actin filaments, microtubules and intermediate filaments.
authors.affiliation: Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Behrens
authors.affiliation: Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
authors.forename: Roxana
authors.initials: R
authors.lastname: Solga
authors.affiliation: Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Ziemann
authors.affiliation: Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany; Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany; Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931Cologne, Germany.
authors.forename: Raphael H
authors.initials: RH
authors.lastname: Rastetter
authors.affiliation: Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Berwanger
authors.affiliation: Institute of Neuropathology, University Hospital Erlangen, 91054 Erlangen, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Herrmann
authors.affiliation: Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany; Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany; Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931Cologne, Germany.
authors.forename: Angelika A
authors.initials: AA
authors.lastname: Noegel
authors.affiliation: Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, Germany. Electronic address: christoph.clemen@uni-koeln.de.
authors.forename: Christoph S
authors.initials: CS
authors.lastname: Clemen
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement; D005347:Fibroblasts / Q000378:metabolism*; D007382:Intermediate Filaments; D051379:Mice; D018345:Mice, Knockout; D008870:Microtubules / Q000378:metabolism*; D054378:Acetogenins
chemical_list: D015107:4-Butyrolactone; C438920:coronin acetogenin; D054378:Acetogenins
keywords: Actin; CRN2; Coronin 1C; Intermediate filaments; Knock-out; Microtubules; Migration; Mitochondria; Primary fibroblasts; Vimentin