title: Protective effect of the methanolic extract of malva parviflora l. leaves on acetic acid-induced ulcerative colitis in rats.
abstract: OBJECTIVE
  Inflammatory bowel disease (IBD) is a general term describing chronic, idiopathic relapsing, inflammatory conditions of the gastrointestinal tract of unknown etiology. Previous studies have indicated that Malva parviflora leaf extract possesses anti-inflammatory, antioxidant, and antiulcerogenic activity. activity. This work aimed to investigatee the anti-inflammatory effect of the methanolic (MEMP) and aqueous (AEMP) extracts of M. parviflora leaves on acetic acid-induced colitis in rats.
  
  
  METHODS
  42 male Wistar albino rats were divided into seven groups (n = 6). Group I: Normal saline control group with no colitis; Group II: Acetic acid colitis group; Group III: 100 mg/kg/5 d MEMP; Group IV: 200 mg/kg/5 d.MEMP; Group V: 100 mg/kg/5 d AEMP; Group VI: 200 mg/kg/5 d AEMP; Group VII: Prednisolone group (2 mg/kg/5 d). Treatments were followed by induction of colitis using intrarectal instillation of 2 mL of 4% acetic acid. Colon damage was evaluated macroscopically (spleen weight/body weight, colon weight/length ratio) and the histological changes were also recorded.
  
  
  RESULTS
  The results of this study showed that acetic acid caused severe inflammation of the colon and a significant increase in spleen weight/body weight, and an increase in colon weight/length ratio compared with normal control group. Pretreatment with MEMP and AEMP for 5 days followed by induction of colitis resulted in a significant attenuation of spleen weight and colon weight/length ratio compared with acetic acid control group. Methanolic extract provided better anticolitic effect than aqueous extract; the effect was prominent at the dose of 200 mg/kg. Histopathological findings confirmed the protective effect of the MEMP.
  
  
  CONCLUSIONS
  In conclusion, MEMP could ameliorate mucosal damage in experimentally induced colitis when given orally.
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, University of Tripoli, Tripoli, Libya.
authors.forename: Aisha
authors.initials: A
authors.lastname: Dugani
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, University of Tripoli, Tripoli, Libya.
authors.forename: Bushra
authors.initials: B
authors.lastname: Dakhil
authors.affiliation: Department of Histology and Medical Genetic, University of Tripoli, Tripoli, Libya.
authors.forename: Soad
authors.initials: S
authors.lastname: Treesh
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D008297:Male; D028501:Malva / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D019342:Acetic Acid; D000432:Methanol