title: Peptide Tag-Induced Horseradish Peroxidase-Mediated Preparation of a Streptavidin-Immobilized Redox-Sensitive Hydrogel.
abstract: Several methods have recently been reported for the preparation of redox-sensitive hydrogels using enzymatic reactions, which are useful for encapsulating sensitive materials such as proteins and cells. However, most of the reported hydrogels is difficult to add further function efficiently, limiting the application of the redox-sensitive hydrogels. In this study, peptide sequences of HHHHHHC and GGGGY (Y-tag) were genetically fused to the N- and C-termini of streptavidin (C-SA-Y), respectively, and C-SA-Y was mixed with horseradish peroxidase and thiol-functionalized 4-arm polyethylene glycol to yield a redox-sensitive C-SA-Y immobilized hydrogel (C-SA-Y gel). The C-SA-Y immobilized in the hydrogel retained its affinity for biotin, allowing for the incorporation of proteins and small molecules to hydrogel via biotin. C-SA-Y gel was further prepared within a water-in-oil (w/o) emulsion system to yield a nanosized hydrogel, to which any intracellular and cytotoxic agent can be modified, making it a potential drug delivery carrier.
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mishina
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Minamihata
authors.affiliation: Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba Center fifth, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Kousuke
authors.initials: K
authors.lastname: Moriyama
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.|Department of Bioengineering, School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D001704:Biopolymers / Q000737:chemistry* / Q000235:genetics; D003432:Cross-Linking Reagents / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005349:Fibroins / Q000737:chemistry* / Q000235:genetics; D005353:Fibronectins / Q000737:chemistry* / Q000235:genetics; D006735:Horseradish Peroxidase / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry*; D010084:Oxidation-Reduction; D010957:Plasmids; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D019809:Streptavidin / Q000737:chemistry*; D013696:Temperature
chemical_list: D001704:Biopolymers; D003432:Cross-Linking Reagents; D005353:Fibronectins; D020100:Hydrogels; D011993:Recombinant Fusion Proteins; C089172:silk-like protein polymer; D005349:Fibroins; D019809:Streptavidin; D006735:Horseradish Peroxidase; D003545:Cysteine