title: Fatal Cowpox Virus Infection in an Aborted Foal.
abstract: The article describes the isolation of a cowpox virus (CPXV) isolate originating from a horse. The skin of a foal, aborted in the third trimester, displayed numerous cutaneous papules. The histological examination showed A-type inclusion bodies within the lesion, typical for CPXV infections. This suspicion was confirmed by real-time PCR where various organs were analyzed. From skin samples, virus isolation was successfully performed. Afterwards, the whole genome of this new isolate "CPXV Amadeus" was sequenced by next-generation technology. Phylogenetic analysis clearly showed that "CPXV Amadeus" belongs to the "CPXV-like 1" clade. To our opinion, the study provides important additional information on rare accidental CPXV infections. From the natural hosts, the voles, species such as rats, cats, or different zoo animals are occasionally infected, but until now only two horse cases are described. In addition, there are new insights toward congenital CPXV infections.
authors.affiliation: 1 Institute of Diagnostic Virology , Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany .
authors.forename: Annika
authors.initials: A
authors.lastname: Franke
authors.affiliation: 2 Department of Veterinary Pathology, Freie Universität Berlin , Berlin, Germany .
authors.forename: Olivia
authors.initials: O
authors.lastname: Kershaw
authors.affiliation: 1 Institute of Diagnostic Virology , Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany .
authors.forename: Maria
authors.initials: M
authors.lastname: Jenckel
authors.affiliation: 2 Department of Veterinary Pathology, Freie Universität Berlin , Berlin, Germany .
authors.forename: Lydia
authors.initials: L
authors.lastname: König
authors.affiliation: 1 Institute of Diagnostic Virology , Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany .
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
authors.affiliation: 1 Institute of Diagnostic Virology , Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany .
authors.forename: Bernd
authors.initials: B
authors.lastname: Hoffmann
authors.affiliation: 1 Institute of Diagnostic Virology , Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany .
authors.forename: Donata
authors.initials: D
authors.lastname: Hoffmann
mesh_terms: D000034:Abortion, Veterinary; D000818:Animals; D015605:Cowpox / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D015608:Cowpox virus / Q000235:genetics / Q000302:isolation & purification*; D017809:Fatal Outcome; D005333:Fetus / Q000821:virology*; D016679:Genome, Viral; D006734:Horse Diseases / Q000473:pathology / Q000821:virology*; D006736:Horses; D010802:Phylogeny
keywords: Cowpox virus; Virology; Zoonosis