title: Physiological influence of silica on germinating pollen as shown by Raman spectroscopy.
abstract: The process of silicification in plants and the biochemical effects of silica in plant tissues are largely unknown. To study the molecular changes occurring in growing cells that are exposed to higher than normal concentration of silicic acid, Raman spectra of germinating pollen grains of three species (Pinus nigra, Picea omorika, and Camellia japonica) were analyzed in a multivariate classification approach that takes into account the variation of biochemical composition due to species, plant tissue structure, and germination condition. The results of principal component analyses of the Raman spectra indicate differences in the utilization of stored lipids, a changed mobilization of storage carbohydrates in the pollen grain bodies, and altered composition and/or structure of cellulose of the developing pollen tube cell walls. These biochemical changes vary in the different species.
authors.affiliation: Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489, Berlin, Germany.|BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Joester
authors.affiliation: Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489, Berlin, Germany.|BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Seifert
authors.affiliation: BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Emmerling
authors.affiliation: Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489, Berlin, Germany.|BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Straße 11, 12489, Berlin, Germany.
authors.forename: Janina
authors.initials: J
authors.lastname: Kneipp
mesh_terms: D028244:Camellia; D016000:Cluster Analysis; D015999:Multivariate Analysis; D028222:Picea; D028223:Pinus; D011058:Pollen / Q000254:growth & development* / Q000378:metabolism*; D025341:Principal Component Analysis; D012822:Silicon Dioxide / Q000378:metabolism*; D013045:Species Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D012822:Silicon Dioxide
keywords: Camellia japonica; Picea omorika; Pinus nigra; Raman spectroscopy; Silica; pollen tube; principal component analysis