title: Formation of starch in plant cells.
abstract: Starch-rich crops form the basis of our nutrition, but plants have still to yield all their secrets as to how they make this vital substance. Great progress has been made by studying both crop and model systems, and we approach the point of knowing the enzymatic machinery responsible for creating the massive, insoluble starch granules found in plant tissues. Here, we summarize our current understanding of these biosynthetic enzymes, highlighting recent progress in elucidating their specific functions. Yet, in many ways we have only scratched the surface: much uncertainty remains about how these components function together and are controlled. We flag-up recent observations suggesting a significant degree of flexibility during the synthesis of starch and that previously unsuspected non-enzymatic proteins may have a role. We conclude that starch research is not yet a mature subject and that novel experimental and theoretical approaches will be important to advance the field.
authors.affiliation: Department of Biology, ETH Zurich, 8092, Zurich, Switzerland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pfister
authors.affiliation: Department of Biology, ETH Zurich, 8092, Zurich, Switzerland. szeeman@ethz.ch.
authors.forename: Samuel C
authors.initials: SC
authors.lastname: Zeeman
mesh_terms: D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D059828:Plant Cells / Q000378:metabolism*; D013213:Starch / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D013213:Starch
keywords: Amylopectin; Amylose; Arabidopsis thaliana; Protein complex formation; Protein phosphorylation