title: Comparison of sepsis rats induced by caecal ligation puncture or Staphylococcus aureus using a LC-QTOF-MS metabolomics approach.
abstract: Sepsis is a whole-body inflammatory response to infection with high mortality and is treated in intensive care units (ICUs). In the present study, to identify metabolic biomarkers that can differentiate sepsis models induced by caecal ligation puncture (CLP) or Staphylococcus aureus (S. aureus), small molecular metabolites in the serum were measured by liquid chromatography quadruple time-of-flight mass spectrometry (LC-QTOF-MS) and analysed using the multivariate statistical analysis (MVA) of partial least square-discrimination analysis (PLS-DA) method. The results demonstrated that the body showed obvious metabolic disorders in the sepsis groups compared with the control group. A total of 8 potential biomarkers were identified in the CLP group, and 10 potential biomarkers were identified in the S. aureus group. These potential biomarkers primarily reflected an energy metabolism disorder, inflammatory response, oxidative stress and tissue damage, which occur during sepsis, and these markers might potentially be used to differentiate CLP from Staphylococcus aureus sepsis.
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lin
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xinru
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; Department of Anesthesiology, Shanghai Ninth People Hospital Affiliated to Shanghai Jiaotong University School of Medicine, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhenglin
authors.initials: Z
authors.lastname: Hu
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Xie
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xianpeng
authors.initials: X
authors.lastname: Zu
authors.affiliation: Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. Electronic address: xmdeng@anesthesia.org.cn.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Deng
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China; School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002432:Cecum / Q000601:surgery; D002853:Chromatography, Liquid / Q000379:methods; D003937:Diagnosis, Differential; D004195:Disease Models, Animal; D008026:Ligation; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D011677:Punctures; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis* / Q000209:etiology; D013047:Specific Pathogen-Free Organisms; D013203:Staphylococcal Infections / Q000097:blood* / Q000175:diagnosis*
chemical_list: D015415:Biomarkers
keywords: Caecal ligation puncture; Liquid chromatography-mass spectrometry; Metabolomics; Potential biomarker; Sepsis; Staphylococcus aureus