title: Myocardin inhibits estrogen receptor alpha-mediated proliferation of human breast cancer MCF-7 cells via regulating MicroRNA expression.
abstract: Myocardin is frequently repressed during human malignant transformation, and restoration of myocardin expression in sarcoma cells contributes to the inhibition of malignant growth. However, its role in breast carcinoma has barely been addressed. Here, we reported that myocardin could inhibit the proliferation of MCF-7 cells. Notably, we show that myocardin inhibited ERα-mediated proliferation of breast cancer MCF-7 via impairing ER-dependent transcriptional activation, mainly through the inhibition of the activity of ERα. Importantly, the molecular mechanism for the inhibition of the ERα-mediated proliferation is that myocardin inhibited the transcription and expression of ERα-induced PCNA, Ki-67, and E2F1 to impair ERα-mediated proliferation of breast cancer MCF-7. Interestingly, myocardin significantly enhanced the transcription and expression of miR-885 depending on the CArG box in miR-885 promoter, and miR-885 targeted the 3' untranslated regions (UTR) of E2F1 to silence the expression of E2F1. Thus, our data provided important and novel insights into how myocardin may deeply influence ERα-mediated breast cancer proliferation. In conclusion, myocardin could be seen as a breast cancer tumor suppressor so that it will provide new ideas for the treatment of breast cancer. © 2016 IUBMB Life, 68(6):477-487, 2016.
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.
authors.forename: Da-Lin
authors.initials: DL
authors.lastname: Lu
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.
authors.forename: Jia-Peng
authors.initials: JP
authors.lastname: Li
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.
authors.forename: Cheng-Xi
authors.initials: CX
authors.lastname: Yu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: De-Liang
authors.initials: DL
authors.lastname: Zheng
authors.affiliation: Medical College, Nanchang University, Jiangxi, People's Republic of China.|Wuhan General Hospital, Guangzhou Military Command, Hubei, People's Republic of China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Hu
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Liao
authors.affiliation: Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, People's Republic of China.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Tong-Cun
authors.initials: TC
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D000099251:Myocardin
chemical_list: D020413:3' Untranslated Regions; D050687:E2F1 Transcription Factor; D047628:Estrogen Receptor alpha; D019394:Ki-67 Antigen; D035683:MicroRNAs; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D015534:Trans-Activators; D000099251:Myocardin; C493753:E2F1 protein, human; C506487:ESR1 protein, human; C574934:MIRN885 microRNA, human
keywords: breast cancer; estrogen receptor alpha; microRNAs; myocardin