title: Osthole inhibits histamine-dependent itch via modulating TRPV1 activity.
abstract: Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as an antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied the molecular mechanism of osthole in histamine-dependent itch by behavioral test, Ca(2+) imaging, and electrophysiological experiments. First, osthole clearly remitted the scratching behaviors of mice induced with histamine, HTMT, and VUF8430. Second, in cultured dorsal root ganglion (DRG) neurons, osthole showed a dose-dependent inhibitory effect to histamine. On the same neurons, osthole also decreased the response to capsaicin and histamine. In further tests, the capsaicin-induced inward currents were inhibited by osthole. These results revealed that osthole inhibited histamine-dependent itch by modulating TRPV1 activity. This study will be helpful in understanding how osthole exerts anti-pruritus effects and suggests that osthole may be a useful treatment medicine for histamine-dependent itch.
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Niu-Niu
authors.initials: NN
authors.lastname: Yang
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Shi
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yu
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Wang
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Zhu
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Yuan
authors.affiliation: Jishou University, Jishou, 410023, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
authors.forename: Tana
authors.initials: T
authors.lastname: Gegen
authors.affiliation: Jishou University, Jishou, 410023, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: He
authors.affiliation: Jishou University, Jishou, 410023, China.
authors.forename: Kehua
authors.initials: K
authors.lastname: Tang
authors.affiliation: College of Life Science, Nanjing Normal University, Nanjing, 210046, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lan
authors.affiliation: College of Basic Medicine, Guangxi University of Chinese Medicine, 13 WuHe Road, Nanning 530299, China.
authors.forename: Guan-Yi
authors.initials: GY
authors.lastname: Wu
authors.affiliation: College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.|Jishou University, Jishou, 410023, China.|The Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, China.
authors.forename: Zong-Xiang
authors.initials: ZX
authors.lastname: Tang
mesh_terms: D000818:Animals; D000982:Antipruritics / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D003374:Coumarins / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology; D006632:Histamine; D015640:Ion Channel Gating / Q000187:drug effects*; D008297:Male; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011537:Pruritus / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D050916:TRPV Cation Channels / Q000378:metabolism*
chemical_list: D000982:Antipruritics; D002121:Calcium Channel Blockers; D003374:Coumarins; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D006632:Histamine; D002211:Capsaicin; D002118:Calcium; C046627:osthol