title: Immunoreactivity of glucose transporter 8 is localized in the epithelial cells of the choroid plexus and in ependymal cells.
abstract: High fructose intake is known to be associated with increased plasma triglyceride concentration, impaired glucose tolerance, insulin resistance, and high blood pressure. In addition, excess fructose intake is also thought to be a risk factor for dementia. Previous immunohistochemical studies have shown the presence of glucose transporter 5 (GLUT5), a major transporter of fructose, in the epithelial cells of the choroid plexus and ependymal cells in the brains of humans, rats, and mice, while GLUT2, a minor transporter of fructose, was localized in the ependymal cells of rat brain. In this study, immunoreactivity for the fructose transporter GLUT8 was observed in the cytoplasm of the epithelial cells in the choroid plexus and in the ependymal cells of the brains of humans and mice. These structures were not immunoreactive for GLUT7, GLUT11, and GLUT12. Our findings support the hypothesis of the transport of intravascular fructose through the epithelial cells of the choroid plexus and the ependymal cells.
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Murakami
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Chiba
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tsuboi
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan.
authors.forename: Machi
authors.initials: M
authors.lastname: Kawauchi
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan.|Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Fujihara
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan.|Department of Perinatology and Gynecology, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Mashima
authors.affiliation: Department of Perinatology and Gynecology, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kanenishi
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan. masaueno@med.kagawa-u.ac.jp.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
mesh_terms: D000818:Animals; D002831:Choroid Plexus / Q000166:cytology* / Q000378:metabolism; D004805:Ependyma / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative / Q000032:analysis* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H
chemical_list: D051246:Glucose Transport Proteins, Facilitative; C405325:SLC2A8 protein, human; C498628:Slc2a8 protein, mouse
keywords: Choroid plexus; Ependymal cell; Fructose; GLUT8