title: [High resolution melting and its application in identity study of traditional Chinese medicine].
abstract: High resolution melting (HRM), based on melting curve analysis, requires not only saturating dyes that fluoresce in the presence of double-stranded DNA, but also higher resolution detection equipment. The melting curve is a novel method for sequence matching, genotyping and mutation scanning. The technology is simple, accurate, rapid, closed-tube, low-cost, and high-throughput, which make it gain more and more applications. This review article presents the basic principles, key factors and both the advantage and limitations of HRM. The potential application is discussed in the study of molecular identity of traditional Chinese medicine.
authors.forename: Kang
authors.initials: K
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000145:classification*; D060005:Genotyping Techniques / Q000379:methods*; D008516:Medicine, Chinese Traditional; D009691:Nucleic Acid Denaturation
chemical_list: D004365:Drugs, Chinese Herbal