title: Relationship Between Chronic Tinnitus and Glial Cell Line-Derived Neurotrophic Factor Gene rs3812047, rs1110149, and rs884344 Polymorphisms in a Turkish Population.
abstract: Glial cell line-derived neurotrophic factor (GDNF) plays a key role in early development of central auditory pathway and the inner ear. However, the auditory pathway studies of GDNF gene polymorphisms are scarce in the literature, and the studies especially associated with tinnitus are limited. Our study aimed to identify whether GDNF gene polymorphisms play any roles in the pathophysiology of tinnitus by investigating the relationship between tinnitus and GDNF polymorphisms. A total of 52 patients with chronic tinnitus and ages ranging from 18 to 55 were admitted to the Ear-Nose-Throat outpatient clinic of Celal Bayar University Medical Faculty Hospital of Manisa, Turkey and constituted the study group. Another 42 patients of the same age range, without tinnitus symptoms and lacking any systemic disease, were also admitted to the clinic and formed the control group. The tympanometric, audiological, and psychoacoustic assessments of the subjects were performed. Deoxyribonucleic acid samples obtained using venous blood taken for routine inspections were used to investigate GDNF gene polymorphisms (rs884344, rs3812047, and rs1110149) by polymerase chain reaction-based restriction fragment length polymorphism method. No correlation could be detected between GDNF rs884344 and rs3812047 polymorphisms and subjects with tinnitus (p > 0.05). Heterozygosity was significantly lower for GDNF rs1110149 polymorphism in tinnitus subjects compared to the controls (p < 0.05). However, the allele frequencies for all 3 polymorphisms were not significantly different between tinnitus and control groups (p > 0.05). Failure to detect correlations between tinnitus and GDNF gene polymorphisms suggests this may be due to the fact that the GDNF gene has a variable expression pattern in different tissues and pathologies. Therefore, the study should be improved and its scope should be expanded by including a larger group of patients and different tissues to investigate the expression pattern of GDNF.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey. sorenay@adu.edu.tr.
authors.forename: Seda
authors.initials: S
authors.lastname: Orenay-Boyacioglu
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Coskunoglu
authors.affiliation: Karyon Biotechnology, Izmir, Turkey.
authors.forename: Zerrin
authors.initials: Z
authors.lastname: Caki
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Fethi Sirri
authors.initials: FS
authors.lastname: Cam
mesh_terms: D000328:Adult; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014012:Tinnitus / Q000235:genetics*; D014421:Turkey; D044465:White People / Q000235:genetics*; D055815:Young Adult
chemical_list: C495039:GDNF protein, human; D051100:Glial Cell Line-Derived Neurotrophic Factor
keywords: Chronic tinnitus; GDNF gene; Neurotrophic factor; Polymorphism