title: Synthesis of amino-rich silica-coated magnetic nanoparticles for the efficient capture of DNA for PCR.
abstract: Magnetic separation has great advantages over traditional bio-separation methods and has become popular in the development of methods for the detection of bacterial pathogens, viruses, and transgenic crops. Functionalization of magnetic nanoparticles is a key factor for efficient capture of the target analytes. In this paper, we report the synthesis of amino-rich silica-coated magnetic nanoparticles using a one-pot method. This type of magnetic nanoparticle has a rough surface and a higher density of amino groups than the nanoparticles prepared by a post-modification method. Furthermore, the results of hydrochloric acid treatment indicated that the magnetic nanoparticles were stably coated. The developed amino-rich silica-coated magnetic nanoparticles were used to directly adsorb DNA. After magnetic separation and blocking, the magnetic nanoparticles and DNA complexes were used directly for the polymerase chain reaction (PCR), without onerous and time-consuming purification and elution steps. The results of real-time quantitative PCR showed that the nanoparticles with higher amino group density resulted in improved DNA capture efficiency. The results suggest that amino-rich silica-coated magnetic nanoparticles are of great potential for efficient bio-separation of DNA prior to detection by PCR.
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Yalong
authors.initials: Y
authors.lastname: Bai
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: USDA-MOST Joint Research Center for Food Safety & Molecular Characterization of Foodborne Pathogens Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
authors.forename: George C
authors.initials: GC
authors.lastname: Paoli
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Shi
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wang
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China; School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Lida
authors.initials: L
authors.lastname: Zhang
authors.affiliation: MOST-USDA Joint Research Center for Food Safety & Bor Luh Food Safety Center, School of Agriculture and Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: xmshi@sjtu.edu.cn.
authors.forename: Xianming
authors.initials: X
authors.lastname: Shi
mesh_terms: D000588:Amines; D004269:DNA, Bacterial / Q000378:metabolism*; D007501:Iron / Q000032:analysis; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
chemical_list: D000588:Amines; D004269:DNA, Bacterial; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D007501:Iron
keywords: Amino-rich; Bio-separation; DNA; Magnetic nanoparticles; PCR