title: Differential remodelling of peroxisome function underpins the environmental and metabolic adaptability of diplonemids and kinetoplastids.
abstract: The remodelling of organelle function is increasingly appreciated as a central driver of eukaryotic biodiversity and evolution. Kinetoplastids including Trypanosoma and Leishmania have evolved specialized peroxisomes, called glycosomes. Glycosomes uniquely contain a glycolytic pathway as well as other enzymes, which underpin the physiological flexibility of these major human pathogens. The sister group of kinetoplastids are the diplonemids, which are among the most abundant eukaryotes in marine plankton. Here we demonstrate the compartmentalization of gluconeogenesis, or glycolysis in reverse, in the peroxisomes of the free-living marine diplonemid, Diplonema papillatum Our results suggest that peroxisome modification was already under way in the common ancestor of kinetoplastids and diplonemids, and raise the possibility that the central importance of gluconeogenesis to carbon metabolism in the heterotrophic free-living ancestor may have been an important selective driver. Our data indicate that peroxisome modification is not confined to the kinetoplastid lineage, but has also been a factor in the success of their free-living euglenozoan relatives.
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Jorge
authors.initials: J
authors.lastname: Morales
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Muneaki
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, UK School of Earth Sciences, University of Bristol, Bristol BS8 1TG, UK.
authors.forename: Tom A
authors.initials: TA
authors.lastname: Williams
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hirawake-Mogi
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Makiuchi
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Tsubouchi
authors.affiliation: Division of Proteomics and Biomolecular Science, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kaga
authors.affiliation: Division of Proteomics and Biomolecular Science, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Taka
authors.affiliation: Division of Proteomics and Biomolecular Science, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fujimura
authors.affiliation: Department of Cellular and Molecular Neuropathology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Koike
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mita
authors.affiliation: Laboratoire de Microbiologie Fondamentale et Pathogénicité (MFP) UMR 5234, Université de Bordeaux, Bordeaux, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bringaud
authors.affiliation: Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida 5101, Venezuela.
authors.forename: Juan L
authors.initials: JL
authors.lastname: Concepción
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: T Martin
authors.initials: TM
authors.lastname: Embley
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan tnara@juntendo.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nara
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D056898:Euglenozoa / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005943:Gluconeogenesis; D008830:Microbodies; D010427:Pentose Phosphate Pathway; D020675:Peroxisomes / Q000378:metabolism*; D010802:Phylogeny; D015398:Signal Transduction; D014349:Trypanosoma cruzi / Q000166:cytology* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004798:Enzymes; D002244:Carbon
keywords: diplonemids; glycolysis; kinetoplastids; metabolic compartmentalization; organelle evolution; peroxisomes