title: Evaluation of an integrated biorefinery based on fractionation of spent sulphite liquor for the production of an antioxidant-rich extract, lignosulphonates and succinic acid.
abstract: Spent sulphite liquor (SSL) has been used for the production of lignosulphonates (LS), antioxidants and bio-based succinic acid. Solvent extraction of SSL with isopropanol led to the separation of approximately 80% of the total LS content, whereas the fermentations carried out using the pretreated SSL with isopropanol led to the production of around 19g/L of succinic acid by both Actinobacillus succinogenes and Basfia succiniciproducens. Fractionation of SSL via nanofiltration to separate the LS and solvent extraction using ethyl acetate to separate the phenolic compounds produced a detoxified sugar-rich stream that led to the production of 39g/L of succinic acid by B. succiniciproducens. This fractionation scheme resulted also in the production of 32.4g LS and 1.15g phenolic-rich extract per 100g of SSL. Both pretreatment schemes removed significant quantities of metals and heavy metals. This novel biorefinery concept could be integrated in acidic sulphite pulping mills.
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Alexandri
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece.
authors.forename: Harris
authors.initials: H
authors.lastname: Papapostolou
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece.
authors.forename: Michael
authors.initials: M
authors.lastname: Komaitis
authors.affiliation: Avecom NV, Industrieweg 122P, 9032 Wondelgem, Belgium.
authors.forename: Lutgart
authors.initials: L
authors.lastname: Stragier
authors.affiliation: Avecom NV, Industrieweg 122P, 9032 Wondelgem, Belgium.
authors.forename: Willy
authors.initials: W
authors.lastname: Verstraete
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece.
authors.forename: Georgios P
authors.initials: GP
authors.lastname: Danezis
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece.
authors.forename: Constantinos A
authors.initials: CA
authors.lastname: Georgiou
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece.
authors.forename: Seraphim
authors.initials: S
authors.lastname: Papanikolaou
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece. Electronic address: akoutinas@aua.gr.
authors.forename: Apostolis A
authors.initials: AA
authors.lastname: Koutinas
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000188:Actinobacillus / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis*; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D005591:Chemical Fractionation / Q000379:methods*; D005285:Fermentation; D005374:Filtration; D008031:Lignin / Q000031:analogs & derivatives* / Q000032:analysis; D036103:Nanotechnology; D012997:Solvents; D019802:Succinic Acid / Q000032:analysis*; D013447:Sulfites / Q000737:chemistry*; D013451:Sulfonic Acids / Q000032:analysis*; D014865:Waste Disposal, Fluid
chemical_list: D000085:Acetates; D000975:Antioxidants; D002241:Carbohydrates; D012997:Solvents; D013447:Sulfites; D013451:Sulfonic Acids; C007650:ethyl acetate; C001545:lignosulfuric acid; D008031:Lignin; D019802:Succinic Acid; D019840:2-Propanol
keywords: Antioxidants; Integrated biorefinery; Lignosulphonates; Spent sulphite liquor; Succinic acid