title: Anti-inflammatory effect of methanolic extract of Conyzacanadensis in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophage cells.
abstract: The aim of this study was to investigate the potential anti-inflammatory effect of Conyzacanadeusis methanol extract (CME) using a cell model of RAW264.7 murine macrophage cell stimulated with lipopolysaccharide (LPS)(1μg/ml). Co-treatment with different concentrations (10, 50 and 100μg/ml) of CME was concentration-dependently reduced the LPS-induced generation of prostaglandin E2 (PGE2), nitric oxide (NO) tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. In addition, CME also reduced the mRNA expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide (iNOS), TNF-α, IL-1β and IL-6 in LPS-stimulated RAW264.7 cells. These results suggested that CME showed an anti-inflammatory activity through reduced the mRNA expression of COX-2, iNOS, TNF-α IL-1β and IL-6 and also decreased the productions of PGE2, NO, TNF-α IL-1β and IL-6in LPS-stimulated RAW264.7 cells.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi, People's Republic of China / Department of Food Science and Nutrition, Pusan National University, Busan, South Korea.
authors.forename: Jia-Le
authors.initials: JL
authors.lastname: Song
authors.affiliation: Department of Food Science and Nutrition, Pusan National University, Busan, South Korea.
authors.forename: Ruo-Kun
authors.initials: RK
authors.lastname: Yi
authors.affiliation: Department of Pharmacy, Northern Jiangsu People's Hospital Affiliated to Yangzhou University (Clinical Medical College of Yangzhou University), Yangzhou, Jiangsu, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification; D002470:Cell Survival / Q000187:drug effects; D005591:Chemical Fractionation; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D000432:Methanol / Q000737:chemistry*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal; D000067996:RAW 264.7 Cells; D012997:Solvents / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012997:Solvents; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D000432:Methanol