title: Analysis of the biocompatibility of perfluoropolyether dimethacrylate network using an organotypic method.
abstract: In this work, we have investigated the potential of perfluoropolyether (PFPE) polymers for use in biomaterial applications, especially in cell culture and tissue engineering. PFPE substrates were synthesized by the photocuring of liquid PFPE urethane dimethacrylate. These surfaces were then modified by ECM protein coatings and microstructuration, to promote cell adhesion and migration. The surface properties of PFPE and PDMS (used as a reference) samples were studied by static contact angle measurements and AFM imaging. Both polymer surfaces were hydrophobic, having sessile air-water contact angles superior to 100°. Collagen and fibronectin coatings were found to change the wettability of PFPE and PDMS samples. The biological testing of substrates was done using a liver organotypic culture to evaluate the migration and density of liver cells. The results over seven days of culture demonstrated that the migration and density of cells cultured under untreated PFPE were higher than the migration and density of cells cultured under PDMS. ECM protein coatings enhanced cell migration from liver explants cultured on PFPE or PDMS. Furthermore, these coatings were more efficient in the case of a PFPE sample. From a second series of tests, in which the PFPE was microstructured, it was found that microstructures promoted the formation of a 3D cell layer. These results indicate that PFPE polymers have a potential for use in the development of biomaterials for tissue engineering and cell culture.
authors.affiliation: CNRS UMR 7338, Laboratoire de Biomécanique et Bioingénierie, Sorbonne Universités, Université de Technologie de Compiègne, France. Electronic address: rachid.jellali@utc.fr.
authors.forename: Rachid
authors.initials: R
authors.lastname: Jellali
authors.affiliation: CNRS UMR 7338, Laboratoire de Biomécanique et Bioingénierie, Sorbonne Universités, Université de Technologie de Compiègne, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Duval
authors.affiliation: CNRS UMR 7338, Laboratoire de Biomécanique et Bioingénierie, Sorbonne Universités, Université de Technologie de Compiègne, France; CNRS UMI 2820, Laboratory for Integrated Micro Mechatronic System, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro ku, Tokyo 153 8505, Japan. Electronic address: eric.leclerc@utc.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Leclerc
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003094:Collagen / Q000737:chemistry; D004987:Ethers / Q000737:chemistry* / Q000494:pharmacology; D005353:Fibronectins / Q000737:chemistry; D005466:Fluorocarbons / Q000737:chemistry* / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D004987:Ethers; D005353:Fibronectins; D005466:Fluorocarbons; D011108:Polymers; C078113:perfluoropolyether; D014867:Water; D003094:Collagen
keywords: ECM proteins; Liver; Microstructuration; Organotypic cultures; Perfluoropolyethers; Tissue engineering