title: SUSCEPTIBILITY LOCI FOR UMBILICAL HERNIA IN SWINE DETECTED BY GENOME-WIDE ASSOCIATION.
abstract: Umbilical hernia (UH) is a complex disorder caused by both genetic and environmental factors. UH brings animal welfare problems and severe economic loss to the pig industry. Until now, the genetic basis of UH is poorly understood. The high-density 60K porcine SNP array enables the rapid application of genome-wide association study (GWAS) to identify genetic loci for phenotypic traits at genome wide scale in pigs. The objective of this research was to identify susceptibility loci for swine umbilical hernia using the GWAS approach. We genotyped 478 piglets from 142 families representing three Western commercial breeds with the Illumina PorcineSNP60 BeadChip. Then significant SNPs were detected by GWAS using ROADTRIPS (Robust Association-Detection Test for Related Individuals with Population Substructure) software base on a Bonferroni corrected threshold (P = 1.67E-06) or suggestive threshold (P = 3.34E-05) and false discovery rate (FDR = 0.05). After quality control, 29,924 qualified SNPs and 472 piglets were used for GWAS. Two suggestive loci predisposing to pig UH were identified at 44.25MB on SSC2 (rs81358018, P = 3.34E-06, FDR = 0.049933) and at 45.90MB on SSC17 (rs81479278, P = 3.30E-06, FDR = 0.049933) in Duroc population, respectively. And no SNP was detected to be associated with pig UH at significant level in neither Landrace nor Large White population. Furthermore, we carried out a meta-analysis in the combined pure-breed population containing all the 472 piglets. rs81479278 (P = 1.16E-06, FDR = 0.022475) was identified to associate with pig UH at genome-wide significant level. SRC was characterized as plausible candidate gene for susceptibility to pig UH according to its genomic position and biological functions. To our knowledge, this study gives the first description of GWAS identifying susceptibility loci for umbilical hernia in pigs. Our findings provide deeper insights to the genetic architecture of umbilical hernia in pigs.
authors.forename: X J
authors.initials: XJ
authors.lastname: Liao
authors.forename: L
authors.initials: L
authors.lastname: Lia
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Long
authors.forename: B
authors.initials: B
authors.lastname: Yang
authors.forename: G R
authors.initials: GR
authors.lastname: Ruan
authors.forename: Y
authors.initials: Y
authors.lastname: Su
authors.forename: H S
authors.initials: HS
authors.lastname: Ai
authors.forename: W C
authors.initials: WC
authors.lastname: Zhang
authors.forename: W Y
authors.initials: WY
authors.lastname: Deng
authors.forename: S J
authors.initials: SJ
authors.lastname: Xiao
authors.forename: J
authors.initials: J
authors.lastname: Ren
authors.forename: N S
authors.initials: NS
authors.lastname: Ding
authors.forename: L S
authors.initials: LS
authors.lastname: Huang
mesh_terms: D000818:Animals; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006554:Hernia, Umbilical / Q000235:genetics* / Q000662:veterinary*; D013552:Swine / Q000235:genetics*; D013553:Swine Diseases / Q000235:genetics*