title: EphA2 silencing promotes growth, migration, and metastasis in salivary adenoid cystic carcinoma: in vitro and in vivo study.
abstract: EphA2 is associated with tumor growth and distant metastasis in numerous human tumors. Considering the controversial effects of EphA2 in different tumors and the lack of reports in salivary adenoid cystic carcinoma (SACC), we evaluated the effects of EphA2 inhibition by short hairpin RNA on SACC through in vivo and in vitro researches for the first time. Real-time reverse transcriptase-PCR and western blot analysis were conducted to verify the interference effect on SACC cells. Using Cell Counting Kit-8, wound healing, Transwell and Matrigel adhesion assays, we confirm that inhibition of EphA2 promotes the migration, invasion and adhesion ability of SACC cells. In vivo research, we prove that silencing of EphA2 significantly accelerates tumor growth and lung metastasis ability by establishing xenograft models in mice, including subcutaneous inoculation and tail vein injection. In addition, immunostaining of EphA2, E-cadherin and Slug from 40 specimens and in vitro simulation of perineural invasion (PNI) assay imply that suppression of EphA2 partially contribute to epithelial-mesenchymal transition and enhancement of PNI in SACC. In conclusion, all the data suggest that EphA2 may act as a tumor suppressor in SACC progression.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University Wuhan, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong, University of Science and Technology Wuhan, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhao
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University Wuhan, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University Wuhan, China.
authors.forename: Ting-Lin
authors.initials: TL
authors.lastname: Yan
authors.affiliation: Department of Stomatology, Liuzhou People's Hospital Guangxi, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Song
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University Wuhan, China.
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Huang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University Wuhan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University Wuhan, China.
authors.forename: Xiao-Cheng
authors.initials: XC
authors.lastname: Zhou
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan University Wuhan, China.
authors.forename: Er-Hui
authors.initials: EH
authors.lastname: Jiang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan UniversityWuhan, China; Department of Oral and Maxillofacial-Head and Neck Oncology, School and Hospital of Stomatology, Wuhan UniversityWuhan, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Shao
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, Wuhan UniversityWuhan, China; Department of Oral and Maxillofacial-Head and Neck Oncology, School and Hospital of Stomatology, Wuhan UniversityWuhan, China.
authors.forename: Zheng-Jun
authors.initials: ZJ
authors.lastname: Shang
keywords: EphA2; RNA interference; Salivary adenoid cystic carcinoma; perineural invasion; tumor progression