title: IgE anti-Haemophilus influenzae type b (Hib) antibodies detected in serum of Hib-vaccinated asthmatic and non-asthmatic pediatric patients.
abstract: BACKGROUND
  Haemophilus influenzae type b (Hib) bacterium causes severe illness in infants and children, but has largely been eliminated by introducing a universal Hib conjugate vaccine. While effects of certain vaccinations on atopic disease have been studied, little is known about the relationship between Hib vaccination and diseases of altered immunoglobulin E (IgE) regulation (asthma or atopy). As such, it is necessary to provide more evidence concerning Hib vaccination as a possible risk factor for atopic disease.
  
  
  METHODS
  Total serum IgE and IgE-and IgG-anti-Hib antibody responses were studied in Hib vaccinated asthmatic (N.=14) and non-asthmatic children (N=26) (VaccZyme™ Human Anti Hib Enzyme Immunoassay Kit). Data are reported as mean optical density (OD) values.
  
  
  RESULTS
  We found that: 1) total serum IgE levels were higher in asthmatic compared with non-asthmatic subjects (389±125 vs. 125±129, P<0.001); 2) IgE and IgG anti-Hib antibody responses were similar in both asthmatic and non-asthmatic subjects (0.722±0.279 and 0.681±0.280, respectively; P=0.65; 0.450±0.505 and 0.573±0.779, respectively; P=0.580).
  
  
  CONCLUSIONS
  The universal Hib vaccine antigen did not result in either increased IgE, or IgG anti-Hib antibody responses in asthmatic or non-asthmatics subjects. Thus, in this cohort, no association between Hib vaccination and asthma status was identified.
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Medical Center, Brooklyn, NY, USA - tamar.smith-norowitz@downstate.edu.
authors.forename: Tamar A
authors.initials: TA
authors.lastname: Smith-Norowitz
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Tehila A
authors.initials: TA
authors.lastname: Saadia
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Natalie
authors.initials: N
authors.lastname: Banniettis
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Yitzchok M
authors.initials: YM
authors.lastname: Norowitz
authors.affiliation: Department of Medicine, Division of Allergy and Immunology, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Rauno
authors.initials: R
authors.lastname: Joks
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Margaret R
authors.initials: MR
authors.lastname: Hammerschlag
authors.affiliation: Department of Pathology, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Helen G
authors.initials: HG
authors.lastname: Durkin
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
authors.forename: Stephan
authors.initials: S
authors.lastname: Kohlhoff
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000097:blood*; D001249:Asthma / Q000276:immunology*; D016667:Bacterial Capsules / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D005260:Female; D006192:Haemophilus Infections / Q000517:prevention & control; D018073:Haemophilus Vaccines / Q000008:administration & dosage* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008297:Male; D014611:Vaccination; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D007074:Immunoglobulin G; D007073:Immunoglobulin E