title: Epidemiology and virulence of VIM-4 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from burn patients in eastern Algeria.
abstract: In this study, we investigated the prevalence of carbapenem-resistant Pseudomonas aeruginosa (CRPA) in burn patients from eastern Algeria, CRPA virulence factors and the molecular epidemiology of CRPA. The overall prevalence of CRPA was 48.38%. Seven (46.66%) isolates were metallo-β-lactamases (MBL) producers and contained the MBL genes blaVIM-4 (n=6) and blaVIM-2 (n=1). Risk factors for CRPA infection were urinary catheter use and intubation (p=0.008). A high percentage of virulence factors (86.6% of these isolates were able to produce protease; 73.3% of isolates has DNase; and 66.6% were haemolysin positive) was observed in CRPA isolates. Among the seven MBL-producing isolates, four had the same clonal profile. The class 1 integrons, which contained the aadA7 gene cassette, were detected in six isolates. The 16SrRNA methylase gene, rmtB, was detected in one strain. All CRPA isolates were biofilm formers. A study on the kinetics of biofilm production revealed that biofilm production increased when the concentration of imipenem or ciprofloxacin and the incubation time increased. This is the first study to report the presence of VIM-4-producing P. aeruginosa from North Africa and also of the high prevalence of CRPA isolates. Based on our study of burn unit patients, the high percentage of P. aeruginosa with virulence factors and multi-drug resistance is alarming.
authors.affiliation: Biochemistry and Applied Microbiology Laboratory, Badji Mokhtar Faculty of Sciences, Department of Biology, Annaba University, Box 12 Sidi Amar, 23000 Annaba, Algeria.
authors.forename: Samah
authors.initials: S
authors.lastname: Meradji
authors.affiliation: Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360 Casablanca, Morocco; Microbiology Laboratory, Faculty of Medicine and Pharmacy, 1 Street Hospital, 20360 Casablanca, Morocco.
authors.forename: Abouddihaj
authors.initials: A
authors.lastname: Barguigua
authors.affiliation: Resuscitation Center and Burns Treatment, University Hospital Ibn Rochd, 23000 Annaba, Algeria.
authors.forename: Mohamed Cherif
authors.initials: MC
authors.lastname: Bentakouk
authors.affiliation: Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360 Casablanca, Morocco; Microbiology Laboratory, Faculty of Medicine and Pharmacy, 1 Street Hospital, 20360 Casablanca, Morocco.
authors.forename: Kaotar
authors.initials: K
authors.lastname: Nayme
authors.affiliation: Microbiology Laboratory, Faculty of Medicine and Pharmacy, 1 Street Hospital, 20360 Casablanca, Morocco.
authors.forename: Khalid
authors.initials: K
authors.lastname: Zerouali
authors.affiliation: Microbiology Laboratory, University Hospital Dorban, 23000 Annaba, Algeria.
authors.forename: Dekhil
authors.initials: D
authors.lastname: Mazouz
authors.affiliation: Biochemistry and Applied Microbiology Laboratory, Badji Mokhtar Faculty of Sciences, Department of Biology, Annaba University, Box 12 Sidi Amar, 23000 Annaba, Algeria.
authors.forename: Houria
authors.initials: H
authors.lastname: Chettibi
authors.affiliation: Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360 Casablanca, Morocco. Electronic address: mohammed.timinouni@pasteur.ma.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Timinouni
mesh_terms: D000328:Adult; D000462:Algeria / Q000453:epidemiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development; D002056:Burns / Q000382:microbiology* / Q000401:mortality; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007440:Intubation / Q000009:adverse effects; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011446:Prospective Studies; D011552:Pseudomonas Infections / Q000453:epidemiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D012307:Risk Factors; D014546:Urinary Catheterization / Q000009:adverse effects; D014774:Virulence; D055815:Young Adult; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D001618:beta-Lactamases; C477893:beta-lactamase bla(VIM-4), Pseudomonas aeruginosa
keywords: Carbapenem resistance; Prevalence; Pseudomonas aeruginosa; Risk factors; VIM