title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα.
abstract: CDDO-Me has exhibited a potent anticancer effect in human esophageal squamous cell carcinoma (ESCC) cells in our previous study, but the molecular interactome remains elusive. We applied the approach of stable-isotope labeling by amino acids in cell culture (SILAC) to assess the proteomic responses of CDDO-Me treatment in human ESCC Ec109 cells. The data were subsequently validated using Western blot assay. The results of our study revealed that CDDO-Me increased the expression level of 543 protein molecules, but decreased the expression level of 709 protein molecules in Ec109 cells. Among these modulated protein molecules, calcium/calmodulin-dependent protein kinase type II subunit α (CaMKIIα) was highly expressed in all tested ESCC cell lines, whereas its expression levels were substantially lower in normal control cell line. Its silencing by small interfering RNA inhibited CDDO-Me induced apoptosis and autophagy in ESCC cells. Collectively, these data demonstrate that the therapeutic response of CDDO-Me in the human ESCC cells is mediated by CaMKIIα.
authors.affiliation: Department of Radiation Oncology, General Hospital of Ningxia Medical UniversityYinchuan 750004, Ningxia, China; Cancer Institute, Ningxia Medical UniversityYinchuan 750004, Ningxia, China.
authors.forename: Yan-Yang
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Graduated School, Ningxia Medical University Yinchuan 750004, Ningxia, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Radiation Oncology, General Hospital of Ningxia Medical UniversityYinchuan 750004, Ningxia, China; Cancer Institute, Ningxia Medical UniversityYinchuan 750004, Ningxia, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Radiation Oncology, General Hospital of Ningxia Medical UniversityYinchuan 750004, Ningxia, China; Cancer Institute, Ningxia Medical UniversityYinchuan 750004, Ningxia, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Hai
authors.affiliation: Department of Radiation Oncology, General Hospital of Ningxia Medical UniversityYinchuan 750004, Ningxia, China; Cancer Institute, Ningxia Medical UniversityYinchuan 750004, Ningxia, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhe
keywords: CDDO-Me; CaMKIIα; SILAC; esophageal squamous cell carcinoma