title: Exosomal Long Noncoding RNAs are Differentially Expressed in the Cervicovaginal Lavage Samples of Cervical Cancer Patients.
abstract: BACKGROUND
  As the second leading cause of cancer morbidity and death in women, cervical cancer remains an important public health problem worldwide. Novel biomarkers with high sensitivity and specificity for the early detection and diagnosis of cervical cancer are urgently needed. Increasing evidence shows that long noncoding RNAs (lncRNAs) are differentially expressed in cancer tissues and may serve as diagnostic markers. In multiple tumor types, exosomes harboring lncRNAs are actively released from tumor cells. In this study, we investigate the potential association of exosomal lncRNA expression with cervical cancer.
  
  
  METHODS
  Cervicovaginal lavage specimens were collected from patients with cervical cancer and cancer-free volunteers who are HPV-positive or HPV-negative. Exosomes in these specimens were isolated by ultracentrifugation and confirmed by transmission electron microscopy. The exosomal lncRNAs HOTAIR, MALAT1, and MEG3 were quantified by qRT-PCR.
  
  
  RESULTS
  Expression of HOTAIR, MALAT1 and MEG3 was predominantly observed in cervical cancer-derived exosomes in cervicovaginal lavage samples. The expression levels of lncRNAs were significantly different in exosomes isolated from cervical cancer patients compared to normal controls.
  
  
  CONCLUSIONS
  Our data suggest that lncRNAs in exosomes isolated from cervicovaginal lavage are differentially expressed in cervical cancer patients and cancer-free volunteers. Exosomal lncRNAs may have great potential to be used for the early detection and diagnosis of cervical cancer, and serve as convenient and noninvasive biomarkers.
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Shuang-Chun
authors.initials: SC
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Luo
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Guo-Xian
authors.initials: GX
authors.lastname: Tao
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Dalian Medical University, Liaoning, China. yuanhonglab@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, China. 89434436@qq.com.
authors.forename: Da-Kang
authors.initials: DK
authors.lastname: Hu
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D055354:Exosomes / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D014625:Vaginal Neoplasms / Q000473:pathology
chemical_list: D062085:RNA, Long Noncoding
keywords: cervical cancer; cervicovaginal lavage; exosome; lncRNA