title: Secondary Metabolites from an Actinomycete from Vietnam's East Sea.
abstract: Analysis of an antimicrobial extract prepared from culture broth of the marine-derived actinomycete Nocardiopsis sp. (strain G057) led to the isolation of twelve compounds, 1-12. Compound 1 (2-[(2R-hydroxypropanoyl)amino]benzamide) was found to be a new enantiomeric isomer while compounds 2 (3-acetyl-4-hydroxycinnoline) and 3 (3,3'-bis-indole) were isolated from a natural source for the first time. The structures of 1-12 were determined by analyses of MS and 2D NMR data. All compounds were evaluated for their antimicrobial activity against a panel of clinically significant microorganisms. Compound 1 selectively inhibited Escherichia coli (MIC: 16 µg/mL). Compounds 2 and 3 exhibited antimicrobial activity against several strains of both Gram-positive and Gram-negative bacteria, and the yeast Candida albicans. Cytotoxic evaluation of compounds 1-3 against four cancer cell lines (KB, LU-1, HepG-2 and MCF-7) indicated that compound 3 produced a weak inhibition against KB and LU cell lines. Two remaining compounds, 1 and 2 were not cytotoxic, even at the concentration of 128 µg/mL.
authors.forename: Quyen Vu
authors.initials: QV
authors.lastname: Thi
authors.forename: Van Hieu
authors.initials: VH
authors.lastname: Tran
authors.forename: Huong Doan Thi
authors.initials: HD
authors.lastname: Mai
authors.forename: Cong Vinh
authors.initials: CV
authors.lastname: Le
authors.forename: Min Le Thi
authors.initials: Mle T
authors.lastname: Hong
authors.forename: Brian T
authors.initials: BT
authors.lastname: Murphy
authors.forename: Van Minh
authors.initials: VM
authors.lastname: Chau
authors.forename: Van Cuong
authors.initials: VC
authors.lastname: Pham
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009792:Oceans and Seas; D012623:Seawater / Q000382:microbiology; D014744:Vietnam; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents