title: The Investigation of a Disintegrin and Metalloproteinase with ThromboSpondin Motifs (ADAMTS) 1, 5 and 16 in Thoracic Aortic Aneurysms and Dissections.
abstract: BACKGROUND
  Disintegrin-like and Metalloproteinase with Thrombospondin Motifs (ADAMTS) proteins that are fundamentally located in the extracellular matrix (ECM) have critical roles on different cellular processes by altering the ECM architecture. It has been known that expression of some members of these proteinases increases in aneurismal and dissectional aortic tissue. The purpose of this study is to investigate ADAMTS1, 5, 16 and tissue inhibitors of metalloproteinases-1, -2 (TIMP-1, -2) levels in aortic tissue obtained from patients with thoracic aortic aneurysms and dissections and to achieve new insights about the function of ADAMTS family members.
  
  
  METHODS
  We investigated ADAMTS1, 5, and 16 expression in human thoracic aortic aneurysms (TAA) (n = 22), thoracic aortic dissections (TAD) (n = 12), and thoracic aortas from age-matched control organ donors (n = 6) (a total number of 34 cases and 6 controls). The expression levels of ADAMTS proteins were determined by Western blot technique using anti-ADAMTS1, ADAMTS5, ADAMTS16, TIMP-1 and TIMP-2 antibodies.
  
  
  RESULTS
  ADAMTS1, 5, and 16 protein expressions were significantly higher in thoracic aortic aneurysm and dissection tissues compared to control aortic tissues. Furthermore, TIMP-1 protein levels decreased in TAA and TAD tissues, TIMP-2 did not change.
  
  
  CONCLUSIONS
  Under the light of our findings, increased expression of ADAMTS1, 5, and 16 proteins may promote deceleration in thoracic aortic aneurysm progression. This is the first study that demonstrates ADAMTS5 and ADAMTS16 proteolytic activity in aneurysm and dissection.
authors.forename: Mahmut Fatih
authors.initials: MF
authors.lastname: Güneş
authors.forename: Mehmet Beşir
authors.initials: MB
authors.lastname: Akpinar
authors.forename: Ismail
authors.initials: I
authors.lastname: Cömertoğlu
authors.forename: Sümeyya
authors.initials: S
authors.lastname: Akyol
authors.forename: Bora
authors.initials: B
authors.lastname: Demirçelik
authors.forename: Özgül Malçok
authors.initials: ÖM
authors.lastname: Gürel
authors.forename: Büşra
authors.initials: B
authors.lastname: Aynekin
authors.forename: Haci Kemal
authors.initials: HK
authors.lastname: Erdemli
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ateş
authors.forename: Beyhan
authors.initials: B
authors.lastname: Eryonucu
authors.forename: Kadir
authors.initials: K
authors.lastname: Demircan
mesh_terms: D051722:ADAM Proteins / Q000032:analysis*; D000071096:ADAMTS Proteins; D000071097:ADAMTS1 Protein; D000071116:ADAMTS5 Protein; D000368:Aged; D000784:Aortic Dissection / Q000378:metabolism*; D017545:Aortic Aneurysm, Thoracic / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human; C510098:ADAMTS16 protein, human; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human