title: Prediction of preeclampsia with angiogenic biomarkers. Results from the prospective Odense Child Cohort.
abstract: OBJECTIVE
  We aimed to investigate how maternal serum soluble Fms-like kinase 1 (sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio prospectively associate to preeclampsia (PE) and clinical subtypes.
  
  
  METHODS
  In an unselected cohort of 1909 pregnant women, sFlt-1 and PlGF were measured with KRYPTOR assays in gestational weeks (GW) 8-14 and 20-34. Associations to PE were assessed by receiver operating characteristics and logistic regression.
  
  
  RESULTS
  Concentrations of sFlt-1, PlGF, and sFlt-1/PlGF in GW20-34 were predictive of PE development, but not in GW8-14. PlGF outperformed sFlt-1/PlGF ratio with an area under curve (AUC) of 0.755 vs. 0.704, p = 0.002. The highest AUC values for PlGF and sFlt-1/PlGF ratio were seen for severe early-onset PE (0.901 and 0.883). Negative predictive values were high for all PE types, but positive predictive values were low.
  
  
  CONCLUSIONS
  PlGF and sFlt-1/PlGF had good predictive value for PE at GW20-34 in a population-based unselected cohort, however with low positive predictive value.
authors.affiliation: a HCA Research, Hans Christian Andersen Children's Hospital, Odense University Hospital , Odense , Denmark.|b Institute of Clinical Research, Faculty of Health Science, University of Southern Denmark , Odense , Denmark.
authors.forename: Louise Bjørkholt
authors.initials: LB
authors.lastname: Andersen
authors.affiliation: c Department of Obstetrics and Gynecology , Odense University Hospital , Odense , Denmark.|d Experimental and Clinical Research Center Max-Delbrueck Center and Charité Berlin , Berlin , Germany.|e Department of Cardiology, Helios-Klinikum Berlin , Berlin , Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Dechend
authors.affiliation: b Institute of Clinical Research, Faculty of Health Science, University of Southern Denmark , Odense , Denmark.|c Department of Obstetrics and Gynecology , Odense University Hospital , Odense , Denmark.
authors.forename: Jan Stener
authors.initials: JS
authors.lastname: Jørgensen
authors.affiliation: a HCA Research, Hans Christian Andersen Children's Hospital, Odense University Hospital , Odense , Denmark.|b Institute of Clinical Research, Faculty of Health Science, University of Southern Denmark , Odense , Denmark.
authors.forename: Birgitte Møller
authors.initials: BM
authors.lastname: Luef
authors.affiliation: f Center for Clinical Epidemiology, Odense University Hospital , Odense , Denmark.
authors.forename: Jan
authors.initials: J
authors.lastname: Nielsen
authors.affiliation: g Department of Clinical Immunology , Odense University Hospital , Odense , Denmark.|h Odense Patient Data Explorative Network (OPEN) , Odense University Hospital , Odense , Denmark.|i Odense Child Cohort, Hans Christian Andersen Children's Hospital , Odense University Hospital , Odense , Denmark.
authors.forename: Torben
authors.initials: T
authors.lastname: Barington
authors.affiliation: a HCA Research, Hans Christian Andersen Children's Hospital, Odense University Hospital , Odense , Denmark.|b Institute of Clinical Research, Faculty of Health Science, University of Southern Denmark , Odense , Denmark.
authors.forename: Henrik Thybo
authors.initials: HT
authors.lastname: Christesen
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D005865:Gestational Age; D006801:Humans; D000072483:Placenta Growth Factor / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011446:Prospective Studies; D012307:Risk Factors; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*
chemical_list: D015415:Biomarkers; D000072483:Placenta Growth Factor; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: PlGF; prediction; preeclampsia; prenatal screening; sFlt-1