title: Intensity Weighted Subtraction Microscopy Approach for Image Contrast and Resolution Enhancement.
abstract: We propose and demonstrate a novel subtraction microscopy algorithm, exploiting fluorescence emission difference or switching laser mode and their derivatives for image enhancement. The key novelty of the proposed approach lies in the weighted subtraction coefficient, adjusted pixel-by-pixel with respect to the intensity distributions of initial images. This method produces significant resolution enhancement and minimizes image distortions. Our theoretical and experimental studies demonstrate that this approach can be applied to any optical microscopy techniques, including label free and non-linear methods, where common super-resolution techniques cannot be used.
authors.affiliation: Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Kseniya
authors.initials: K
authors.lastname: Korobchevskaya
authors.affiliation: Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Peres
authors.affiliation: Advanced Robotics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Plasmachem GmbH, Rudower Chaussee, 29, D-12489, Berlin, Germany.
authors.forename: Alexei
authors.initials: A
authors.lastname: Antipov
authors.affiliation: Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Colin J R
authors.initials: CJ
authors.lastname: Sheppard
authors.affiliation: Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Diaspro
authors.affiliation: Nanoscopy, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bianchini