title: Regulation of neural stem cells by choroid plexus cells population.
abstract: The choroid plexus is a tissue on the central nervous system responsible for producing cerebrospinal fluid, maintaining homeostasis and neural stem cells support; though, all of its functions still unclear. This study aimed to demonstrate the niches of choroid plexus cells for a better understanding of the cell types and functions, using the porcine as the animal model. The collected material was analyzed by histology, immunohistochemistry, and cell culture. The cell culture was characterizated by immunocytochemistry and flow cytometry. Our results showed OCT-4, TUBIII, Nestin, CD45, CD73, CD90 positive expression and GFAP, CD105 negative expression, also methylene blue histological staining confirmed the presence of telocytes cells. We realized that the choroid plexus is a unique and incomparable tissue with different niches of cells as pluripotent, hematopoietic, neuronal progenitors and telocyte cells, which provide its complexity, differentiated functionality and responsibility on brain balance and neural stem cells regulation.
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP, Brazil. Electronic address: kelly.roballo@usp.br.
authors.forename: Kelly C S
authors.initials: KC
authors.lastname: Roballo
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP, Brazil. Electronic address: natgoncalves@usp.br.
authors.forename: Natalia J N
authors.initials: NJ
authors.lastname: Gonçalves
authors.affiliation: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, SP, Brazil. Electronic address: nairagodoy@gmail.com.
authors.forename: Naira C G
authors.initials: NC
authors.lastname: Pieri
authors.affiliation: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, SP, Brazil. Electronic address: alinesouza25@hotmail.com.
authors.forename: Aline F
authors.initials: AF
authors.lastname: Souza
authors.affiliation: Department of Animal Reproduction, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Sao Paulo, SP, Brazil. Electronic address: andrefc@usp.br.
authors.forename: André F C
authors.initials: AF
authors.lastname: Andrade
authors.affiliation: Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP, Brazil. Electronic address: ceambrosio@usp.br.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Ambrósio
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000166:cytology* / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D013552:Swine; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D064231:Nestin; D050814:Octamer Transcription Factor-3; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens; D015720:5'-Nucleotidase
keywords: Brain; Fetuses; Functionality; Pluripotent cell; Swine