title: Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants.
abstract: In this study, we constructed dual-transgene vectors (pDT1, pDT7, and pDT7G) that simultaneously co-expressed two genes in plants. ACTIN2 and UBQ10 promoters were used to control the expression of these two genes. The 4×Myc, 3×HA, and 3×Flag reporter genes allowed for the convenient identification of a tunable co-expression system in plants, whereas the dexamethasone (Dex) inducible reporter gene C-terminus of the glucocorticoid receptor (cGR) provided Dex-dependent translocation of the fusion gene between the nucleus and cytoplasm. The function of pDT vectors was validated using four pairwise genes in Nicotiana benthamiana or Arabidopsis thaliana. The co-expression efficiency of two genes from the pDT1 and pDT7G vectors was 35% and 42%, respectively, which ensured the generation of sufficient transgenic materials. These pDT vectors are simple, reliable, efficient, and time-saving tools for the co-expression of two genes through a single transformation event and can be used in the study of protein-protein interactions or multi-component complexes.
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China; Department of Molecular, Cell & Developmental Biology, University of California, Los Angeles, CA 90095, USA.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Molecular, Cell & Developmental Biology, University of California, Los Angeles, CA 90095, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Plant Sciences, Jilin University, Changchun 130062, China.
authors.forename: Mingdi
authors.initials: M
authors.lastname: Bian
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China.
authors.forename: Reqing
authors.initials: R
authors.lastname: He
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Yan
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China. Electronic address: xiaoyzhao@hnu.edu.cn.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China. Electronic address: xmL05@hnu.edu.cn.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D063188:Rana clamitans / Q000235:genetics; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics*
keywords: Co-expression; Dual-transgene vector; pDT1; pDT7; pDT7G