title: Anticentromere antibody-positive primary Sjögren's syndrome: Epitope analysis of a subset of anticentromere antibody-positive patients.
abstract: OBJECTIVE
  Anticentromere antibody (ACA) is generally considered to be a serological marker for systemic sclerosis (SSc). ACA-positive patients with primary Sjögren's syndrome (pSS) have also been reported. ACA often recognizes centromere proteins (CENPs): CENP-A, CENP-B, and CENP-C, and sometimes reacts to heterochromatin protein 1 (HP1)α. We compared the reactivity against six different epitopes for three ACA-positive clinical subgroups: 29 patients with pSS, 36 SSc patients with sicca symptoms, and 28 SSc patients without sicca symptoms.
  
  
  METHODS
  We utilized enzyme-linked immunosorbent assays (ELISAs) with recombinant proteins covering six different epitope regions of ACA (the amino terminus (Nt) of CENP-A, CENP-B, and CENP-C, the carboxyl terminus (Ct) of CENP-B and CENP-C, and HP1α).
  
  
  RESULTS
  The patients with pSS were found to have IgG-class autoantibodies against CENP-C-Nt and HP1α, and IgA-class autoantibodies against CENP-C-Ct with significantly higher frequencies than the SSc patients with or without sicca symptoms. The positive predictive value and the negative predictive value of the combination of these three autoantibodies for pSS were 73% and 82%, respectively, for pSS.
  
  
  CONCLUSIONS
  Based on the result that reactivities against CENP-C and HP1α in patients with pSS differ from those in patients with SSc, we propose ACA-positive pSS as a clinical subset of SS that is independent of SSc.
authors.affiliation: a Department of Dermatology , Nagoya University Graduate School of Medicine , Nagoya , Aichi , Japan.
authors.forename: Noriyo
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: a Department of Dermatology , Nagoya University Graduate School of Medicine , Nagoya , Aichi , Japan.
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Muro
authors.affiliation: b Division of Rheumatology , Kanazawa University Hospital , Kanazawa , Ishikawa , Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: c Rheumatic Disease Center, Kurashiki Medical Center , Kurashiki, Okayama , Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Nishiyama
authors.affiliation: d Division of Environmental Medicine , National Defense Medical College Research Institute , Saitama , Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Takada
authors.affiliation: e Division of Rheumatology, Department of Internal Medicine , Hyogo College of Medicine , Nishinomiya , Hyogo , Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sekiguchi
authors.affiliation: e Division of Rheumatology, Department of Internal Medicine , Hyogo College of Medicine , Nishinomiya , Hyogo , Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Hashimoto
authors.affiliation: f Department of Rheumatology and Clinical Immunology , Kyoto University Graduate School of Medicine , Kyoto , Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ohmura
authors.affiliation: g Division of Immunology and Rheumatology, Department of Medicine 3 , Hamamatsu University School of Medicine , Hamamatsu , Shizuoka , Japan , and.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Shimoyama
authors.affiliation: h Department of Pathology , Tsurumi University School of Dental Medicine , Yokohama , Kanagawa , Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Saito
authors.affiliation: b Division of Rheumatology , Kanazawa University Hospital , Kanazawa , Ishikawa , Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kawano
authors.affiliation: a Department of Dermatology , Nagoya University Graduate School of Medicine , Nagoya , Aichi , Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Akiyama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D015415:Biomarkers; D002503:Centromere / Q000276:immunology*; D000076247:Centromere Protein A; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D012859:Sjogren's Syndrome / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; D015415:Biomarkers; C000715594:CBX5 protein, human; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D000939:Epitopes; D011994:Recombinant Proteins; C538950:anticentromere antibody; D000090266:Chromobox Protein Homolog 5
keywords: Anticentromere antibody; Centromere protein C; Epitope; Heterochromatin protein 1α; Primary Sjögren's syndrome