title: Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells.
abstract: Regardless of the known anti-inflammatory potential of heterocyclic antidepressants, the mechanisms concerning their modulating effects are not completely known. In our earlier work, maprotiline, a heterocyclic antidepressants, considerably inhibited infiltration of polymorphonuclear cell leucocytes into the inflamed paw. To understand the mechanism involved, we evaluated the effect of vascular cell adhesion molecule (VCAM-1), intracellular adhesion molecule (ICAM-1) expression in stimulated endothelial cells. Endothelial cells were stimulated with lipopolysaccharide (LPS) in the presence and absence of maprotiline (10(-8) to 10(-6) M) and ICAM-1 and VCAM-1 expression were measured using real-time quantitative reverse transcription polymerase chain reaction. Maprotiline significantly decreased the LPS-induced expression of VCAM-1 at all applied concentrations. The expression of ICAM-1 decreased in the presence of maprotiline at 10(-6) M concentration (P<0.05). Since maprotiline inhibits the expression of adhesion molecules, ICAM-1 and VCAM-1 in LPS-stimulated human endothelial cells, it can be a possible way through which maprotiline exerts its anti-inflammatory properties.
authors.affiliation: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Laleh
authors.initials: L
authors.lastname: Rafiee
authors.affiliation: Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Valiollah
authors.initials: V
authors.lastname: Hajhashemi
authors.affiliation: Applied Physiology Research Center and Department of Physiology, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Shaghayegh Haghjooy
authors.initials: SH
authors.lastname: Javanmard
keywords: Endothelial cells; ICAM-1; Maprotiline; VCAM-1