title: Comparative Transcriptome Analysis Reveals Different Silk Yields of Two Silkworm Strains.
abstract: Cocoon and silk yields are the most important characteristics of sericulture. However, few studies have examined the genes that modulate these features. Further studies of these genes will be useful for improving the products of sericulture. JingSong (JS) and Lan10 (L10) are two strains having significantly different cocoon and silk yields. In the current study, RNA-Seq and quantitative polymerase chain reaction (qPCR) were performed on both strains in order to determine divergence of the silk gland, which controls silk biosynthesis in silkworms. Compared with L10, JS had 1375 differentially expressed genes (DEGs; 738 up-regulated genes and 673 down-regulated genes). Nine enriched gene ontology (GO) terms were identified by GO enrichment analysis based on these DEGs. KEGG enrichment analysis results showed that the DEGs were enriched in three pathways, which were mainly associated with the processing and biosynthesis of proteins. The representative genes in the enrichment pathways and ten significant DEGs were further verified by qPCR, the results of which were consistent with the RNA-Seq data. Our study has revealed differences in silk glands between the two silkworm strains and provides a perspective for understanding the molecular mechanisms determining silk yield.
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.|The Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang Jiangsu 212018, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Qin
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors.forename: Huanjun
authors.initials: H
authors.lastname: Yu
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yu
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.|The Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang Jiangsu 212018, China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Hou
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China.|The Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang Jiangsu 212018, China.
authors.forename: Muwang
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D012831:Bombyx / Q000145:classification / Q000235:genetics*; D013045:Species Specificity; D059467:Transcriptome