title: Oxidized Dextran Enhances Alternative Activation of Macrophages in Mice of Opposite Lines.
abstract: Differences in peritoneal macrophage polarization in mice of opposite lines CBA and C57Bl/6 and the effects of 60 kDa oxidized dextran were studied. Macrophages of C57Bl/6 mice demonstrated a phenotype close to M1, with increasing expression of CD86 costimulatory molecule and unchanged CD206 expression in response to activation. Macrophages of CBA mice demonstrated higher plasticity in response to activating agents; expression of the markers increased irrespectively on stimulated receptor (TLR-4 or mannose receptor) and both CD86 (classical activation) and CD206 (alternative activation) increased. Macrophage response to addition of oxidized dextran (60 kDa) to the culture medium could be characterized as potentiation of their alternative activation: expression of CD86 in CBA mice in response to LPS and LPS+IL-4 and in C57Bl/6 mice in response to IFN-γ and LPS+IFN-γ decreased, while expression of CD206 by intact macrophages of CBA mice and by macrophages stimulated by IFN-γ and IL-4 increased under the effect of 60 kDa oxidized dextran.
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Chechushkov
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: P M
authors.initials: PM
authors.lastname: Kozhin
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: N S
authors.initials: NS
authors.lastname: Zaitseva
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Lemza
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia. lemen@centercem.ru.
authors.forename: E B
authors.initials: EB
authors.lastname: Men'shchikova
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Troitskii
authors.affiliation: Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.|Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Shkurupy
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D016764:Cell Polarity; D003911:Dextrans / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D037181:Lectins, C-Type / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D003911:Dextrans; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface
keywords: 60 kDa oxidized dextran; C57Bl/6 and CBA mice; alternative activation; classical activation; peritoneal macrophages