title: An unbiased nuclear proteomics approach reveals novel nuclear protein components that participates in MAMP-triggered immunity.
abstract: (MAMP)-triggered immunity (MTI) is the first layer of molecular defense encountered by pathogens. Genetic screens have contributed to our knowledge of MTI, but are limited to phenotype-causing mutations. Here we attempt to identify novel factors involved in the early event leading to plant MTI by comparing the nuclear proteomes of two Arabidopsis genotypes treated with chitosan. Our approach revealed that following chitosan treatment, cerk1 plants had many nuclear accumulating proteins in common, but also some unique ones, when compared with Col-0 plants. Analysis of the identified proteins revealed a nuclear accumulation of DNA-modifying enzymes, RNA-binding proteins and ribosomal proteins. Our results demonstrate that nuclear proteomic is a valid, phenotype-independent approach to uncover factor involved in cellular processes.
authors.affiliation: a Department of Chemistry , Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières , Trois-Rivières , QC , Canada.
authors.forename: Zainab
authors.initials: Z
authors.lastname: Fakih
authors.affiliation: a Department of Chemistry , Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières , Trois-Rivières , QC , Canada.
authors.forename: Md Bulbul
authors.initials: MB
authors.lastname: Ahmed
authors.affiliation: a Department of Chemistry , Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières , Trois-Rivières , QC , Canada.
authors.forename: Claire
authors.initials: C
authors.lastname: Letanneur
authors.affiliation: a Department of Chemistry , Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières , Trois-Rivières , QC , Canada.
authors.forename: Hugo
authors.initials: H
authors.lastname: Germain
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D048271:Chitosan / Q000494:pharmacology; D002853:Chromatography, Liquid; D063990:Gene Ontology; D009687:Nuclear Proteins / Q000378:metabolism*; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000378:metabolism*; D057865:Plant Immunity / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; D000069452:Pathogen-Associated Molecular Pattern Molecules; D048271:Chitosan; C525882:CERK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: Arabidopsis; MAMP triggered immunity; cerk1; chitosan; nucleus; proteomic