title: Simultaneous quantitation of the diastereoisomers of scholarisine and 19-epischolarisine, vallesamine, and picrinine in rat plasma by supercritical fluid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study.
abstract: Dengtaiye tablet has been used to treat chronic bronchitis cough. Scholarisine, 19-epischolarisine, vallesamine, and picrinine are the representative constituents of Dengtaiye. A rapid and sensitive assay based on supercritical fluid chromatography with tandem mass spectrometry has been developed and validated for the determination of the diastereoisomers of scholarisine and 19-epischolarisine, vallesamine, and picrinine in rat plasma using lamotrigine as internal standard. The analysis in a run time of only 6 min was performed on an ACQUITY UPC(2) Trefoil(TM) BEH 2-EP column (3.0 × 150 mm, 2.5 μm) at 50ºC. The mobile phase consisting of carbon dioxide and methanol (2 mM ammonium formate) was performed as follows: 15% methanol (2 mM ammonium formate) maintained at 0-2 min, 15-19% methanol (2 mM ammonium formate) at 2-4 min, 19-15% methanol (2 mM ammonium formate) at 4-6 min. The flow rate was 1.50 mL/min. The assay was linear over the concentration ranges 50-10000 pg/mL for scholarisine, 19-epischolarisine, vallesamine, and picrinine with corresponding lower limits of quantitation of 50 pg/mL. Intra- and interday precisions were in the range 1.42-12.85% with accuracies in the range -11.71-2.48%. The method was successfully applied to a pharmacokinetic study involving a single oral administration of 108 mg/kg Dengtaiye tablet to rats.
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.
authors.forename: Chunsu
authors.initials: C
authors.lastname: Liang
authors.affiliation: Waters Corporation, Shanghai, China.
authors.forename: Yongwei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Waters Corporation, Shanghai, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.|Clinical Pharmacology Center, Research Institute of Translational Medicine, The First Hospital of Jilin University, Changchun, PR China.
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D025924:Chromatography, Supercritical Fluid; D026121:Indole Alkaloids / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D015394:Molecular Structure; D051381:Rats; D013237:Stereoisomerism; D013607:Tablets / Q000008:administration & dosage / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry
chemical_list: D026121:Indole Alkaloids; D013607:Tablets; C528220:picrinine; C528219:scholarisine A
keywords: 19-Epischolarisine; Picrinine; Scholarisine; Supercritical fluid chromatography; Vallesamine