title: Comparison of the expression of human equilibrative nucleotide transporter 1 (hENT1) and ribonucleotide reductase subunit M1 (RRM1) genes in seven non-Hodgkin lymphoma cell lines.
abstract: We investigated the variability in the expression of human equilibrative nucleoside transporter 1 (hENT1) and ribonucleotide reductase subunit M1 (RRM1) in non-Hodgkin lymphoma cell lines. hENT1 and RRM1 mRNA expression levels in natural killer (NK) cells and seven non-Hodgkin lymphoma cell lines (YTS, SNK-6, Jeko-1, ly-1, Raji, Karpas, and Jurket) were studied using reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) and the results were compared using the Student t-test. mRNA expression of hENT1 was detectable in YTS, SNK-6, Jeko-1, ly-1, Raji, Karpas, Jurket, and NK cells, which revealed variability in gene expression. There were significant differences in the mRNA expression values of hENT1 (P = 0.021) and RRM1 (P = 0.002) compared to those in NK cells. mRNA expression of both hENT1 and RRM1 was closely associated with non-Hodgkin lymphoma cell proliferation. Differential expression analysis of hENT1 and RRM1 in non-Hodgkin lymphoma cell lines may provide novel drug leads for precision medicine.
authors.affiliation: Shangqiu Medical College, Shangqiu, China.|The First Clinical Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Zhao
authors.affiliation: Shangqiu Medical College, Shangqiu, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: The First Clinical Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: F
authors.initials: F
authors.lastname: Shi
authors.affiliation: The First Clinical Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Zhang
authors.affiliation: The First Clinical Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: W L
authors.initials: WL
authors.lastname: Xue
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D033721:Equilibrative Nucleoside Transporter 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000378:metabolism; D012262:Ribonucleoside Diphosphate Reductase; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D033721:Equilibrative Nucleoside Transporter 1; C438649:SLC29A1 protein, human; D025521:Tumor Suppressor Proteins; C475286:RRM1 protein, human; D012262:Ribonucleoside Diphosphate Reductase