title: Association Between Amplification and Expression of C-MYC Gene and Clinicopathological Characteristics of Stomach Cancer.
abstract: BACKGROUND
  The incidence rate of gastric cancer in western countries has shown a remarkable decline in the recent years while it is still the most common cancer among males in Iran. The proto-oncogene MYC, located at 8q24.1, regulates almost 15% of human genes and is activated in 20% of all tumors. The amplification of MYC and overexpression of its protein product are observed in 15 - 30% of gastric neoplasias.
  
  
  OBJECTIVE
  The objective of this study was to find the preferences of Chromogenic In Situ Hybridization (CISH) and Immunohistochemistry (IHC) in diagnosis and prognosis of gastric cancer.
  
  
  METHODS
  We studied 102 samples of gastric cancer in Iran and all the patients had undergone primary surgical resection at the Cancer Institute Hospital, Tehran University of Medical Sciences. The CISH and IHC techniques were applied for all our samples. All of the samples had adenocarcinoma gastric cancer and were selected randomly. Also, the type of study was cross sectional. The sample size was 100 patients.
  
  
  RESULTS
  Our data revealed that both diffuse and intestinal types of gastric cancer occurred significantly more in males than females. Our results showed that there was an indication of some correlation between grades and CISH, although the difference was not significant. Our data also showed that CISH positive patients (43%) were more frequent compared to IHC positive patients (14.7%). There was a correlation between CISH and IHC. These results revealed that there was a significant difference between grades and IHC. There was also no statistical difference between CISH amplification in diffuse and intestinal types.
  
  
  CONCLUSIONS
  From the results, it could be concluded that for administration of the treatment of stomach cancer, and progress and prognosis of tumor, which is important for patients and clinicians, the CISH is a better and more feasible test than IHC, in regards to sensitivity and specificity.
authors.affiliation: Department of Medical Genetics, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Malihea
authors.initials: M
authors.lastname: Khaleghian
authors.affiliation: Department of Pathology, Immunohistochemistry Laboratory, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Issa
authors.initials: I
authors.lastname: Jahanzad
authors.affiliation: Department of Medical Genetics, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shakoori
authors.affiliation: Department of Pathology, Iran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Amirnader
authors.initials: A
authors.lastname: Emami Razavi
authors.affiliation: Department of Medical Genetics, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Cyrus
authors.initials: C
authors.lastname: Azimi
keywords: Adenocarcinoma; C-MYC; Chromogenic In Situ hybridization (CISH); Immunohistochemistry (IHC); Stomach