title: Production of ω-hydroxy octanoic acid with Escherichia coli.
abstract: The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like precursors. For this, we used the model organism Escherichia coli and increased primarily the release of the octanoic acid precursors by overexpressing the plant thioesterase FatB2 from Cuphea hookeriana in a β-oxidation deficient strain, which lead to the production of 2.32mM (8.38mggcww(-1)) octanoic acid in 24h. In order to produce the corresponding ω-hydroxy derivative, we additionally expressed the engineered self-sufficient monooxygenase fusion protein CYP153AMaq(G307A)-CPRBM3 within the octanoic acid producing strain. With this, we finally produced 234μM (0.95mggcww(-1)) ω-hydroxy octanoic acid in a 20h fed-batch set-up.
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Marko
authors.initials: M
authors.lastname: Kirtz
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Janosch
authors.initials: J
authors.lastname: Klebensberger
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Konrad B
authors.initials: KB
authors.lastname: Otte
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Sven M
authors.initials: SM
authors.lastname: Richter
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany. Electronic address: bernhard.hauer@itb.uni-stuttgart.de.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hauer
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D002210:Caprylates / Q000032:analysis / Q000378:metabolism*; D031562:Cuphea / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism
chemical_list: D002210:Caprylates; D010940:Plant Proteins; D013869:Thiolester Hydrolases
keywords: E. coli; Fatty acids; Monooxygenase; Octanoic acid; Thioesterase; ω-hydroxy octanoic acid