title: Ethanol extract of Cotinus coggygria leaves accelerates wound healing process in diabetic rats.
abstract: BACKGROUND
  Cotinus coggygria Scop. (Anacardiaceae) leaves that were used as wound healing in traditional Balkan and Anatolian folk medicine, could be potentially effective in treating diabetic wounds.
  
  
  OBJECTIVE
  This study investigates biochemical and histological effects of ethanol extract of C. coggygria (CCE) on excision wound model in diabetic rats.
  
  
  METHODS
  This study was conducted on diabetic Wistar albino rats, which were injected by a single dose (50 mg/kg i.p.) streptozotocin. Afterward an excision wound model was created in all animals; diabetic control rats were applied topically simple ointment and diabetic treatment rats were applied topically 5% (w/w) ointment with CC, once a day during the experimental period. Malondialdehyde, glutathione and hydroxyproline levels in wound tissues were investigated at the end of 3rd, 7th, and 14th days. Histopathological examination was also performed.
  
  
  RESULTS
  Hydroxyproline content was significantly increased in the CCE treated group versus control after the 3rd and 7th days (15.33 versus 11.83; 19.67 versus 15.67 mg/g, p < 0.05; respectively). A statistically significant elevation in glutathione at the end of 3rd, 7th, and 14th days (5.13 versus 1.58, p < 0.05; 4.72 versus 1.88, p < 0.05; 3.83 versus 1.88 μmol/g, p < 0.05, respectively) and a statistically significant decrease in malondialdehyde level at the end of 7th day (4.49 versus 1.48 nmol/g, p < 0.05) were determined in the treated group versus control group. These results were also supported by histological analyses.
  
  
  CONCLUSIONS
  These findings indicate that CCE accelerated the cutaneous wound healing process in diabetic wounds, in confirmation of its traditional use.
authors.affiliation: a Department of Biochemistry, Faculty of Pharmacy , Marmara University , İstanbul , Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Aksoy
authors.affiliation: b Department of Pharmacognosy , Marmara University , İstanbul , Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Sen
authors.affiliation: c Department of Clinical Pharmacy , Marmara University , İstanbul , Turkey.
authors.forename: Mesut
authors.initials: M
authors.lastname: Sancar
authors.affiliation: a Department of Biochemistry, Faculty of Pharmacy , Marmara University , İstanbul , Turkey.
authors.forename: Turgut
authors.initials: T
authors.lastname: Sekerler
authors.affiliation: d Department of Histology-Embryology, Faculty of Medicine , Marmara University , İstanbul , Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Akakin
authors.affiliation: b Department of Pharmacognosy , Marmara University , İstanbul , Turkey.
authors.forename: Leyla
authors.initials: L
authors.lastname: Bitis
authors.affiliation: a Department of Biochemistry, Faculty of Pharmacy , Marmara University , İstanbul , Turkey.
authors.forename: Fikriye
authors.initials: F
authors.lastname: Uras
authors.affiliation: e Department of Pharmaceutical Botany, Faculty of Pharmacy , Istanbul University , Istanbul , Turkey.
authors.forename: Sukran
authors.initials: S
authors.lastname: Kultur
authors.affiliation: c Department of Clinical Pharmacy , Marmara University , İstanbul , Turkey.
authors.forename: Fikret Vehbi
authors.initials: FV
authors.lastname: Izzettin
mesh_terms: D027926:Anacardiaceae; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D020556:Neutrophil Infiltration; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D017208:Rats, Wistar; D013311:Streptozocin; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D008315:Malondialdehyde; D013311:Streptozocin; D005978:Glutathione
keywords: Anacardiaceae; diabetes; excision wound; hydroxyproline