title: Genetic Features of MCR-1-Producing Colistin-Resistant Escherichia coli Isolates in South Africa.
abstract: A series of colistin-resistant Escherichia coli clinical isolates was recovered from hospitalized and community patients in South Africa. Seven clonally unrelated isolates harbored the mcr-1 gene located on different plasmid backbones. Two distinct plasmids were fully sequenced, and identical 2,600-bp-long DNA sequences defining a mcr-1 cassette were identified. Promoter sequences responsible for the expression of mcr-1, deduced from the precise identification of the +1 transcription start site for mcr-1, were characterized.
authors.affiliation: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland INSERM European Unit (LEA) (INSERM, Paris, France), University of Fribourg, Fribourg, Switzerland laurent.poirel@unifr.ch.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.affiliation: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Kieffer
authors.affiliation: Department of Clinical Microbiology, Ampath National Laboratory Services, Milpark Hospital, Johannesburg, South Africa.
authors.forename: Adrian
authors.initials: A
authors.lastname: Brink
authors.affiliation: Department of Clinical Microbiology and Molecular Biology, Ampath National Laboratory Services, National Referral Laboratory, Centurion, South Africa.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Coetze
authors.affiliation: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Jayol
authors.affiliation: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland INSERM European Unit (LEA) (INSERM, Paris, France), University of Fribourg, Fribourg, Switzerland University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D013019:South Africa; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; D003091:Colistin