title: Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats.
abstract: Non-insulin-dependent diabetes mellitus (NIDDM) is associated with chronic inflammatory activity and disrupted insulin signaling, leading to insulin resistance (IR). The present study investigated the benefits of neurolytic celiac plexus block (NCPB) on IR in a rat NIDDM model. Goto-Kakizaki rats fed a high-fat, high-glucose diet to induce signs of NIDDM were randomly divided into NCPB and control groups; these received daily bilateral 0.5% lidocaine or 0.9% saline injections into the celiac plexus, respectively. Following 14 and 28 daily injections, rats were subject to oral glucose tolerance tests (OGTTs) or sacrificed for the analysis of serum free fatty acids (FFAs), serum inflammatory cytokines and skeletal muscle insulin signaling. Compared with controls, rats in the NCPB group demonstrated significantly (P<0.05) lower baseline, 60-min and 120-min OGTT values, lower 120-min serum insulin, lower IR [higher insulin sensitivity index (ISI1) and lower ISI2) and lower serum FFAs, tumor necrosis factor-α, interleukin (IL)-1β and IL-6. Conversely, NCPB rats exhibited higher basal and insulin-stimulated skeletal muscle glucose uptake and higher skeletal muscle insulin receptor substrate-1 (IRS-1) and glucose transporter type 4 expression. There were no differences between the groups in insulin receptor β (Rβ) or Akt expression; however Rβ-Y1162/Y1163 and Akt-S473 phosphorylation levels were higher and IRS-1-S307 phosphorylation were lower in NCPB rats than in the controls. These results indicate that NCPB improved insulin signaling and reduced IR, possibly by inhibiting inflammatory cytokine release.
authors.affiliation: Department of Anesthesiology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Medical Laboratory Center, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: General Surgery Center, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Urology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.affiliation: Medical Laboratory Center, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Development and Regeneration Key Laboratory of Sichuan, Chengdu Medical College, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Bingyin
authors.initials: B
authors.lastname: Su
authors.affiliation: Department of ICU, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors.forename: Fuxiang
authors.initials: F
authors.lastname: Li
keywords: Goto-Kakizaki rats; insulin resistance; insulin signal transduction pathway; neurolytic celiac plexus block