title: Bioengineering novel floating nanoparticles for protein and drug delivery.
abstract: Gas vesicle nanoparticles (GVNPs) are hollow protein nanoparticles produced byHalobacteriumsp. NRC-1 which are being engineered for protein delivery. To advance the bioengineering potential of GVNPs, a strain of NRC-1 deleted for thegvpC gene (ΔgvpC) was constructed and a synthetic gene coding forGaussia princepsluciferase was fused to an abbreviatedgvpC gene on an expression plasmid. When introduced into theΔgvpC strain, an active GvpC-luciferase fusion protein bound to GVNPs resulted. These results represent both a technical improvement in the GVNP display system and its expansion for the display of active enzymes.
authors.affiliation: University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA.
authors.forename: Priya
authors.initials: P
authors.lastname: DasSarma
authors.affiliation: University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA.
authors.forename: Ram
authors.initials: R
authors.lastname: Karan
authors.affiliation: University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA ; PuKyong National University, YongSoro 45, Busan 608-737, Korea.
authors.forename: Jong-Myoung
authors.initials: JM
authors.lastname: Kim
authors.affiliation: University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA ; University of Baltimore, 1420 N. Charles St., Baltimore, MD 21201, USA.
authors.forename: Wolf
authors.initials: W
authors.lastname: Pecher
authors.affiliation: University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA.
authors.forename: Shiladitya
authors.initials: S
authors.lastname: DasSarma
keywords: Gas vesicle naoparticles (GVNPs); Gaussia princeps; enzyme display; luciferase; vaccine