title: Three-step channel conformational changes common to DNA packaging motors of bacterial viruses T3, T4, SPP1, and Phi29.
abstract: The DNA packaging motor of dsDNA bacterial viruses contains a head-tail connector with a channel for the genome to enter during assembly and to exit during host infection. The DNA packaging motor of bacterial virus phi29 was recently reported to use the "One-way revolving" mechanism for DNA packaging. This raises a question of how dsDNA is ejected during infection if the channel acts as a one-way inward valve. Here we report a three step conformational change of the portal channel that is common among DNA translocation motors of bacterial viruses T3, T4, SPP1, and phi29. The channels of these motors exercise three discrete steps of gating, as revealed by electrophysiological assays. The data suggest that the three step channel conformational changes occur during DNA entry process, resulting in a structural transition in preparation for DNA movement in the reverse direction during ejection.
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Department of Physiology & Cell Biology, College of Medicine, and Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA; College of Pharmacy, Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY, USA.
authors.forename: Shaoying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Department of Physiology & Cell Biology, College of Medicine, and Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA; College of Pharmacy, Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY, USA.
authors.forename: Zhouxiang
authors.initials: Z
authors.lastname: Ji
authors.affiliation: College of Pharmacy, Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY, USA.
authors.forename: Erfu
authors.initials: E
authors.lastname: Yan
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Department of Physiology & Cell Biology, College of Medicine, and Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA; College of Pharmacy, Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY, USA.
authors.forename: Farzin
authors.initials: F
authors.lastname: Haque
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Department of Physiology & Cell Biology, College of Medicine, and Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA; College of Pharmacy, Nanobiotechnology Center, Markey Cancer Center, University of Kentucky, Lexington, KY, USA. Electronic address: guo.1091@osu.edu.
authors.forename: Peixuan
authors.initials: P
authors.lastname: Guo
mesh_terms: D017103:Bacillus Phages / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017903:Bacteriophage T3 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017122:Bacteriophage T4 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D042003:DNA Packaging; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019065:Virus Assembly
chemical_list: D004279:DNA, Viral
keywords: Biomotor; Nano channel; Nanobiotechnology; Nanomotor; Nanopore; Nanotechnology; Single channel conductance; Viral assembly