title: Characterization of Multiple Heat-Shock Protein Transcripts from Cydia pomonella: Their Response to Extreme Temperature and Insecticide Exposure.
abstract: The economically important fruit pest Cydia pomonella (L.) exhibits a strong adaptability and stress tolerance to environmental stresses. Heat-shock proteins (HSPs) play key roles in insects in coping with environmental stresses. However, little is known about the spatiotemporal expression patterns of HSPs and their response to stresses in C. pomonella. In this study, a thermal treatment-recovery test was performed, and the expression profiles of a novel isolated HSP, named CpHSP40, and six CpHSPs were determined. Third-instar larvae were able to recover from cold shock (0 °C) and heat shock (40 °C). Escherichia coli BL21 (DE3) cells harboring recombinant pET-28a (+)-CpHSP40 plasmid showed significant temperature tolerance. CpHSPs were developmentally and tissue-specifically expressed. The responses of CpHSPs to 0 and 40 °C (with or without recovery) and insecticide exposure were varied. All of these indicated that the expression of HSPs plays a role in the development and in environmental adaptation in C. pomonella.
authors.affiliation: Key Laboratory of Economical and Applied Entomology of Liaoning Province, College of Plant Protection, Shenyang Agricultural University , Shenyang 110866, People's Republic of China.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University , Yangling 712100, Shaanxi, People's Republic of China.
authors.forename: Ya-Lin
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Economical and Applied Entomology of Liaoning Province, College of Plant Protection, Shenyang Agricultural University , Shenyang 110866, People's Republic of China.
authors.forename: Xiao-Qi
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Economical and Applied Entomology of Liaoning Province, College of Plant Protection, Shenyang Agricultural University , Shenyang 110866, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: Key Laboratory of Economical and Applied Entomology of Liaoning Province, College of Plant Protection, Shenyang Agricultural University , Shenyang 110866, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
authors.affiliation: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101, People's Republic of China.
authors.forename: Ling-Yi
authors.initials: LY
authors.lastname: Jia
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009036:Moths / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013696:Temperature
chemical_list: D006360:Heat-Shock Proteins; D019476:Insect Proteins; D007306:Insecticides
keywords: adaptation; development; heat-shock proteins; stress; tolerance