title: Beet western yellows virus infects the carnivorous plant Nepenthes mirabilis.
abstract: Although poleroviruses are known to infect a broad range of higher plants, carnivorous plants have not yet been reported as hosts. Here, we describe the first polerovirus naturally infecting the pitcher plant Nepenthes mirabilis. The virus was identified through bioinformatic analysis of NGS transcriptome data. The complete viral genome sequence was assembled from overlapping PCR fragments and shown to share 91.1 % nucleotide sequence identity with the US isolate of beet western yellows virus (BWYV). Further analysis of other N. mirabilis plants revealed the presence of additional BWYV isolates differing by several insertion/deletion mutations in ORF5.
authors.affiliation: Plant Advanced Technologies SA, 13, Rue du bois de la Champelle, 54500, Vandœuvre-lès-Nancy, France.
authors.forename: Sissi
authors.initials: S
authors.lastname: Miguel
authors.affiliation: INRA UMR 1121, Laboratoire Agronomie et Environnement, 2 avenue de la forêt de Haye TSA 40602, 54518, Vandœuvre-lès-Nancy, France.|Laboratoire Agronomie et Environnement, Université de Lorraine UMR 1121, 2 avenue de la forêt de Haye TSA 40602, 54518, Vandœuvre-lès-Nancy, France.
authors.forename: Flore
authors.initials: F
authors.lastname: Biteau
authors.affiliation: Plant Advanced Technologies SA, 13, Rue du bois de la Champelle, 54500, Vandœuvre-lès-Nancy, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Mignard
authors.affiliation: UMR Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, 71 Avenue, Edouard Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France.
authors.forename: Armelle
authors.initials: A
authors.lastname: Marais
authors.affiliation: UMR Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, 71 Avenue, Edouard Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Candresse
authors.affiliation: UMR Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, 71 Avenue, Edouard Bourlaux, CS 20032, 33882, Villenave d'Ornon Cedex, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Theil
authors.affiliation: INRA UMR 1121, Laboratoire Agronomie et Environnement, 2 avenue de la forêt de Haye TSA 40602, 54518, Vandœuvre-lès-Nancy, France.|Laboratoire Agronomie et Environnement, Université de Lorraine UMR 1121, 2 avenue de la forêt de Haye TSA 40602, 54518, Vandœuvre-lès-Nancy, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bourgaud
authors.affiliation: INRA UMR 1121, Laboratoire Agronomie et Environnement, 2 avenue de la forêt de Haye TSA 40602, 54518, Vandœuvre-lès-Nancy, France. Alain.Hehn@univ-lorraine.fr.|Laboratoire Agronomie et Environnement, Université de Lorraine UMR 1121, 2 avenue de la forêt de Haye TSA 40602, 54518, Vandœuvre-lès-Nancy, France. Alain.Hehn@univ-lorraine.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Hehn
mesh_terms: D000818:Animals; D001483:Base Sequence; D027461:Beta vulgaris / Q000821:virology; D052679:Luteoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019684:Magnoliopsida / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: BWYV; Carnivorous plant; NGS; Nepenthes; Polerovirus; Transcriptome