title: Oxidative damage in gills and liver in Nile tilapia (Oreochromis niloticus) exposed to diazinon.
abstract: Agricultural activity demands the use of pesticides for plague control and extermination. In that matter, diazinon is one of the most widely used organophosphorus pesticides (OPs). Despite its benefits, the use of OPs in agricultural activities can also have negative effects since the excessive use of these substances can represent a major contamination problem for water bodies and organisms that inhabit them. The aim of this paper was to evaluate oxidative damage in lipids and proteins of Nile tilapia (Oreochromis niloticus) exposed acutely to diazinon (0.97, 1.95 and 3.95ppm) for 12 or 24h. The evaluation of oxidative damage was determined by quantifying lipid hydroperoxides (Fox method) and oxidized proteins (DNPH method). The data from this study suggest that diazinon induces a concentration-dependent oxidative damage in proteins, but not lipids, of the liver and gills of Nile tilapia. Furthermore, the treatment leads to a decrease in the concentration of total proteins, which can have serious consequences in cell physiology and fish development.
authors.affiliation: Universidad Autónoma de Nayarit, Laboratorio de Inmunotoxicología, Cd de la Cultura Amado Nervo s/n, z.p. 63000, Tepic Nayarit, México.
authors.forename: G A
authors.initials: GA
authors.lastname: Toledo-Ibarra
authors.affiliation: Universidad Autónoma de Nayarit, Laboratorio de Inmunotoxicología, Cd de la Cultura Amado Nervo s/n, z.p. 63000, Tepic Nayarit, México.
authors.forename: K J G
authors.initials: KJG
authors.lastname: Díaz Resendiz
authors.affiliation: Universidad Autónoma de Nayarit, Unidad Académica de Ciencias Químico-Biológicas y Farmacéuticas, Cd de la Cultura Amado Nervo s/n, z.p 63000 Tepic, Nayarit, México; Centro Nayarita de Innovación y Transferencia de Tecnología A.C. (CENiT2), Laboratorio de Contaminación Biológica, Cd Industrial, Tepic Nayarit, México.
authors.forename: G H
authors.initials: GH
authors.lastname: Ventura-Ramón
authors.affiliation: Hospital ISSSTE "Aquiles Calles Ramírez", Paseo de la Loma s/n, Tepic, Nayarit, México.
authors.forename: F
authors.initials: F
authors.lastname: González-Jaime
authors.affiliation: Laboratorio de Toxicología Ambiental, Escuela Nacional de Ciencias Biológicas- Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Unidad Profesional Zacatenco, Ciudad de México.
authors.forename: A
authors.initials: A
authors.lastname: Vega-López
authors.affiliation: Departamento de Psicoinmunología, Instituto Nacional de Psiquiatría "Ramón de la Fuente", Calzada México-Xochimilco 101, Col. San Lorenzo Huipilco, Tlalpan, 14370, Ciudad de México, México.
authors.forename: E
authors.initials: E
authors.lastname: Becerril-Villanueva
authors.affiliation: Departamento de Psicoinmunología, Instituto Nacional de Psiquiatría "Ramón de la Fuente", Calzada México-Xochimilco 101, Col. San Lorenzo Huipilco, Tlalpan, 14370, Ciudad de México, México.
authors.forename: L
authors.initials: L
authors.lastname: Pavón
authors.affiliation: Universidad Autónoma de Nayarit, Laboratorio de Inmunotoxicología, Cd de la Cultura Amado Nervo s/n, z.p. 63000, Tepic Nayarit, México. Electronic address: ivan_giron@hotmail.com.
authors.forename: M I
authors.initials: MI
authors.lastname: Girón-Pérez
mesh_terms: D000818:Animals; D023681:Cichlids / Q000378:metabolism*; D003976:Diazinon / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000378:metabolism; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010575:Pesticides / Q000633:toxicity; D050050:Protein Carbonylation / Q000187:drug effects; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D029941:Fish Proteins; D008054:Lipid Peroxides; D010575:Pesticides; D014874:Water Pollutants, Chemical; D003976:Diazinon
keywords: Diazinon; Gills; Liver; Nile tilapia; Organophosphorus pesticide; Oxidative damage