title: Application of a quantitative (1)H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan.
abstract: Quantitative (1)H-NMR ((1)H-qNMR) was applied to the determination of paeonol concentration in Moutan cortex, Hachimijiogan, and Keishibukuryogan. Paeonol is a major component of Moutan cortex, and its purity was calculated from the ratio of the intensity of the paeonol H-3' signal at δ 6.41 ppm in methanol-d 4 or 6.40 ppm in methanol-d 4 + TFA-d to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material grade. As a result, the paeonol content in two lots of Moutan cortex as determined by (1)H-qNMR was found to be 1.59 % and 1.62 %, respectively, while the paeonol content in Hachimijiogan and Keishibukuryogan was 0.15 % and 0.22 %, respectively. The present study demonstrated that the (1)H-NMR method is useful for the quantitative analysis of crude drugs and Kampo formulas.
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.|National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Tanaka
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.
authors.forename: Hikari
authors.initials: H
authors.lastname: Shibata
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan. anagatsu@kinjo-u.ac.jp.
authors.forename: Akito
authors.initials: A
authors.lastname: Nagatsu
mesh_terms: D000098:Acetophenones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D020835:Medicine, Kampo; D029596:Paeonia / Q000737:chemistry; D011786:Quality Control
chemical_list: D000098:Acetophenones; D004365:Drugs, Chinese Herbal; C054591:hachimijiogan; C054593:keishibukuryogan; C038881:moutan cortex; C013638:paeonol
keywords: Hachimijiogan; Keishibukuryogan; Moutan cortex; Paeonol; Quantitative 1H-NMR; Reagent purity