title: Detection of Superior Markers for Polymerase Chain Reaction Diagnosis of Breast Cancer Micrometastasis in Sentinel Lymph Nodes.
abstract: Breast cancer is the most prevalent type of cancer among women around the world, and mortality is primarily caused by micro-metastatic disease. The complex mechanisms of breast cancer invasion and metastasis are intrinsically related to the malignant cell type so that early detection of micro-metastases can help prolongation of survival for patient. The aim of the present research work was evaluation of the expression status of mammoglobin protein as a candidate molecular marker in the negative sentinel lymph node (SLN). Fifty tumor specimens, and 50 normal adjacent breast tissue samples from the same patients were selected on the basis of having more than 10% tumor content for RNA extraction from SLNs. Tumor samples and normal adjacent breast tissue were archived in the form of frozen fresh tissue in liquid nitrogen. Real-time PCR was performed on a Bioner life express gradient thermal cycler system. Mammoglobin gene overexpression in breast cancer metastasis was investigated. Single marker results were mammaglobin 66.7% and CK19 50.0%, with 58.3% for the two in combination. Due to improved outcome with at least 3 genes (83.3%), it seems, triple marker evaluation will be most likely useful for detecting micro-metastases instead of studying separate genes.
authors.affiliation: Department of Medical Laboratory Science, Midwifery-Nursing Institute, Islamic Azad University of Chalous Branch, Chalous, Iran E-mail: Movafagh.a@sbmu.ac.ir.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Alizadeh Shargh
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
authors.forename: Nosratolah
authors.initials: N
authors.lastname: Zarghami
authors.forename: Arezou
authors.initials: A
authors.lastname: Sayad
authors.forename: Neda
authors.initials: N
authors.lastname: Mansouri
authors.forename: Mohammad
authors.initials: M
authors.lastname: Taheri
authors.forename: Atefeh
authors.initials: A
authors.lastname: Heidary Pour
authors.forename: Mostafa
authors.initials: M
authors.lastname: Iranpour
authors.forename: Hamid
authors.initials: H
authors.lastname: Ghaedi
authors.forename: Vahid
authors.initials: V
authors.lastname: Montazeri
authors.forename: Nilofar
authors.initials: N
authors.lastname: Massoudi
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.forename: S A
authors.initials: SA
authors.lastname: Mortazavi-Tabatabaei
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008207:Lymphatic Metastasis; D061206:Neoplasm Micrometastasis; D009363:Neoplasm Proteins / Q000235:genetics*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D000071036:Sentinel Lymph Node / Q000378:metabolism / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins