title: Post-conditioning anti-PUMA treatment protects mice against mice heart I/R injury.
abstract: PUMA is a pro-apoptotic gene, which has been found to be critical to the pathogenesis during heart ischemia-reperfusion injury (IRI). We investigate whether anti-PUMA protect mice from acute heart failure.
  
  
  
  Mice were subjected to 30 min ischemia and 24 hrs reperfusion in the presence or absence of anti-PUMA. Treated mice were evaluated for heart PUMA protein and mRNA expression, and apoptosis by terminal deoxy nucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) staining.
  
  
  
  In mice, anti-PUMA post conditioning markedly reduced PUMA mRNA and protein expression in the heart 4-fold. Hearts from mice that received anti-PUMA had substantially fewer heart muscles apoptosis by TUNEL staining. Anti-PUMA post-conditioning greatly reduced infarct size to 14.4±3.7%, from 38.2±3.9% in the untreated I/R group. Furthermore, survival experiments revealed that more than 90% of control mice died from lethal I/R, whereas 20% of the anti-PUMA post-treated mice survived until the end of the 10-day observation period.
  
  
  
  This study confirms the importance of PUMA-mediated apoptosis in heart ischemia-reperfusion injury. Silencing PUMA by recombinant PUMA has therapeutic promise to limit ischemia-reperfusion injury.
authors.affiliation: Department of Anesthesia, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. chuhaichenqd@126.com.
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: W-L
authors.initials: WL
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Ma
authors.forename: H-C
authors.initials: HC
authors.lastname: Chu
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D006321:Heart; D020287:In Situ Nick-End Labeling; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism; D025521:Tumor Suppressor Proteins
chemical_list: D051017:Apoptosis Regulatory Proteins; C488588:PUMA protein, mouse; D025521:Tumor Suppressor Proteins