title: Doxorubicin and cisplatin induce apoptosis in ovarian stromal cells obtained from cryopreserved human ovarian tissue.
abstract: OBJECTIVE
  To investigate mechanisms by which doxorubicin (DOX) and cisplatin (CIS) cause human ovarian stroma injury.
  
  
  METHODS
  Stromal cells from human cryopreserved ovarian tissue were cultured in the presence of 1 µM DOX and 10 µM CIS. Ovarian damage induced by treatments was evaluated by 'Live/Dead' and sulforhodamine-B assays, the expression of different apoptosis markers.
  
  
  RESULTS
  Stromal cell growth was inhibited by DOX and CIS, and this effect was accompanied by apoptosis through mitochondrial pathway activation: Bax, cleaved-caspase 9, cleaved-PARP1 induction and Akt1, Bcl2, phospho-44/42-MAPK/ERK1/2 reduction were observed.
  
  
  CONCLUSIONS
  DOX and CIS induced apoptosis in human ovarian stromal cells. Knowledge of mechanisms by which the drugs act is important to identify possible ways to counteract side effects of chemotherapy on ovaries.
authors.affiliation: Gynecology & Pathophysiology of Human Reproductive Unit, Department of Medical & Surgical Sciences, University of Bologna, S Orsola-Malpighi Hospital, Bologna, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Fabbri
authors.affiliation: Gynecology & Pathophysiology of Human Reproductive Unit, Department of Medical & Surgical Sciences, University of Bologna, S Orsola-Malpighi Hospital, Bologna, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Macciocca
authors.affiliation: Gynecology & Pathophysiology of Human Reproductive Unit, Department of Medical & Surgical Sciences, University of Bologna, S Orsola-Malpighi Hospital, Bologna, Italy.
authors.forename: Rossella
authors.initials: R
authors.lastname: Vicenti
authors.affiliation: Gynecology & Pathophysiology of Human Reproductive Unit, Department of Medical & Surgical Sciences, University of Bologna, S Orsola-Malpighi Hospital, Bologna, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Paradisi
authors.affiliation: Department of Biomedicine & Prevention, University of Rome Tor Vergata, Rome, Italy.
authors.forename: Francesca Gioia
authors.initials: FG
authors.lastname: Klinger
authors.affiliation: Surgical Pathology, Department of Experimental, Diagnostic & Speciality Medicine, University of Bologna, S Orsola-Malpighi Hospital, Bologna, Italy.
authors.forename: Gianandrea
authors.initials: G
authors.lastname: Pasquinelli
authors.affiliation: Department of Biological, Geological, & Environmental Science, University of Bologna, Bologna, Italy.
authors.forename: Enzo
authors.initials: E
authors.lastname: Spisni
authors.affiliation: Gynecology & Pathophysiology of Human Reproductive Unit, Department of Medical & Surgical Sciences, University of Bologna, S Orsola-Malpighi Hospital, Bologna, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Seracchioli
authors.affiliation: Department of Biological, Geological, & Environmental Science, University of Bologna, Bologna, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Papi
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000009:adverse effects*; D015925:Cryopreservation; D004317:Doxorubicin / Q000009:adverse effects*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission; D010053:Ovary / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D004317:Doxorubicin; D002945:Cisplatin
keywords: cisplatin; doxorubicin; human ovarian stromal cells