title: Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology.
abstract: The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants, and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyond genome editing. Here, we review the most recent applications of the CRISPR/Cas toolkit in plants and also discuss key factors for improving CRISPR/Cas performance and strategies for reducing the off-target effects. Novel technical breakthroughs in mammalian research regarding the CRISPR/Cas toolkit will also be incorporated into this review in hope to stimulate prospective users from the plant research community to fully explore the potential of these technologies.
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Zhenxiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: lijfeng3@mail.sysu.edu.cn.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Li
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D018745:Genome, Plant / Q000235:genetics; D023281:Genomics; D006801:Humans; D010944:Plants / Q000235:genetics*
keywords: CRISPR/Cas technology; Gene knockout; Genome editing; Plants