title: Ultrastructural alterations of oligodendrocytes in prefrontal white matter in schizophrenia: A post-mortem morphometric study.
abstract: Neuroimaging studies showed abnormalities in frontal white matter (WM) in schizophrenia that were associated with clinical symptoms. Previously, we reported ultrastructural alterations of myelinated fibers and reduction in the numerical density of oligodendrocytes in BA 10 WM in patients with schizophrenia. We aimed to perform a qualitative and morphometric study of the ultrastructure of oligodendrocytes in BA 10 WM in schizophrenia and in normal controls.
  
  
  
  The study was performed using electron microscopy and morphometry. Size, volume density (Vv) and the number (N) of organelles of oligodendrocytes were estimated in 21 patients with schizophrenia and 20 normal controls. The data were examined using the Kolmogorov-Smirnov test for normality. Pearson correlation analysis was performed to assess possible correlations between the parameters measured and age, post-mortem interval, neuroleptic treatment and duration of the disease. Comparisons between the schizophrenia patients and controls were performed using ANCOVA tests.
  
  
  
  We found oligodendrocyte swelling, vacuolation, paucity of ribosomes and mitochondria and accumulation of lipofuscin granules in schizophrenia as compared to controls. Morphometry detected a significant reduction in Vv and N of mitochondria and the increase in Vv and N of lipofuscin granules and vacuoles in oligodendrocytes in the schizophrenic group as compared to controls.
  
  
  
  Alterations of oligodendrocytes in schizophrenia provide evidence for the disturbance of their energy, lipid and protein metabolism in prefrontal WM. Oligodendrocyte abnormalities might disturb axonal integrity and circuitry and contribute to the pathophysiology of schizophrenia.
authors.affiliation: Laboratory of Clinical Neuropathology, Mental Health Research Center, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Vikhreva
authors.affiliation: Laboratory of Clinical Neuropathology, Mental Health Research Center, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Rakhmanova
authors.affiliation: Laboratory of Clinical Neuropathology, Mental Health Research Center, Moscow, Russia.
authors.forename: D D
authors.initials: DD
authors.lastname: Orlovskaya
authors.affiliation: Laboratory of Clinical Neuropathology, Mental Health Research Center, Moscow, Russia. Electronic address: uranovan@mail.ru.
authors.forename: N A
authors.initials: NA
authors.lastname: Uranova
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D008928:Mitochondria / Q000648:ultrastructure; D009836:Oligodendroglia / Q000648:ultrastructure*; D017397:Prefrontal Cortex / Q000648:ultrastructure*; D012559:Schizophrenia / Q000473:pathology*; D014617:Vacuoles / Q000648:ultrastructure; D066127:White Matter / Q000648:ultrastructure*
keywords: Morphometry; Oligodendrocyte; Postmortem; Schizophrenia; Ultrastructure; White matter