title: Effective asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate by recombinant E. coli CCZU-A13 in [Bmim]PF6-hydrolyzate media.
abstract: It was the first report that the concentrated hydrolyzates from the enzymatic hydrolysis of dilute NaOH (3wt%)-soaking rice straw at 30°C was used to form [Bmim]PF6-hydrolyzate (50:50, v/v) media for bioconverting ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl (R)-4-chloro-3-hydroxybutanoate [(R)-CHBE] (>99% e.e.) with recombinant E. coli CCZU-A13. Compared with pure glucose, the hydrolyzates could promote both initial reaction rate and the intracellular NADH content. Furthermore, emulsifier OP-10 (20mM) was employed to improve the reductase activity. Moreover, Hp-β-cyclodextrin (0.01mol Hp-β-cyclodextrin/mol COBE) was also added into this bioreaction system for enhancing the biosynthesis of (R)-CHBE from COBE by E. coli CCZU-A13 whole-cells. The yield of (R)-CHBE (>99% e.e.) from 800mM COBE was obtained at 100% in the [Bmim]PF6-hydrolyzate (50:50, v/v) media by supplementation of OP-10 (20mM) and Hp-β-CD (8mM). In conclusion, an effective strategy for the biosynthesis of (R)-CHBE was successfully demonstrated.
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, Changzhou University, Changzhou, China; Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, USA. Electronic address: heyucai2001@163.com.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Tao
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Di
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Lin-Bing
authors.initials: LB
authors.lastname: Zhang
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Zhang
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Gang-Gang
authors.initials: GG
authors.lastname: Chong
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Jiang
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China; Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, USA.
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
mesh_terms: D000090:Acetoacetates / Q000378:metabolism*; D001711:Biotransformation; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D052578:Ionic Liquids / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: C511345:4-chloro-3-hydroxybutyric acid; D000090:Acetoacetates; D003470:Culture Media; D006885:Hydroxybutyrates; D052578:Ionic Liquids; C111982:ethyl 4-chloro-3-oxobutanoate
keywords: Dilute NaOH pretreatment; E. coli CCZU-A13; Ethyl (R)-4-chloro-3-hydroxybutanoate; Ethyl 4-chloro-3-oxobutanoate; Rice straw