title: Characterisation of the bacterial community structures in the intestine of Lampetra morii.
abstract: The metagenomic analysis and 16S rDNA sequencing method were used to investigate the bacterial community in the intestines of Lampetra morii. The bacterial community structure in L. morii intestine was relatively simple. Eight different operational taxonomic units were observed. Chitinophagaceae_unclassified (26.5 %) and Aeromonas spp. (69.6 %) were detected as dominant members at the genus level. The non-dominant genera were as follows: Acinetobacter spp. (1.4 %), Candidatus Bacilloplasma (2.5 %), Enterobacteria spp. (1.5 %), Shewanella spp. (0.04 %), Vibrio spp. (0.09 %), and Yersinia spp. (1.8 %). The Shannon-Wiener (H) and Simpson (1-D) indexes were 0.782339 and 0.5546, respectively. The rarefaction curve representing the bacterial community richness and Shannon-Wiener curve representing the bacterial community diversity reached asymptote, which indicated that the sequence depth were sufficient to represent the majority of species richness and bacterial community diversity. The number of Aeromonas in lamprey intestine was two times higher after stimulation by lipopolysaccharide than PBS. This study provides data for understanding the bacterial community harboured in lamprey intestines and exploring potential key intestinal symbiotic bacteria essential for the L. morii immune response.
authors.affiliation: Lamprey Research Center, School of Life Science, Liaoning Normal University, Liushu South Street No. 1, Dalian, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Lamprey Research Center, School of Life Science, Liaoning Normal University, Liushu South Street No. 1, Dalian, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Xie
authors.affiliation: Lamprey Research Center, School of Life Science, Liaoning Normal University, Liushu South Street No. 1, Dalian, China. liqw@263.net.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D000069196:Gastrointestinal Microbiome; D007422:Intestines / Q000382:microbiology*; D007798:Lampreys / Q000382:microbiology*; D056186:Metagenomics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Aeromonas; Bacterial community; Intestine; Lamprey; Metagenomics