title: Clinical implications of cytosine deletion of exon 5 of P53 gene in non small cell lung cancer patients.
abstract: OBJECTIVE
  Lung cancer is considered to be the most common cancer in the world. In humans, about 50% or more cancers have a mutated tumor suppressor p53 gene thereby resulting in accumulation of p53 protein and losing its function to activate the target genes that regulate the cell cycle and apoptosis. Extensive research conducted in murine cancer models with activated p53, loss of p53, or p53 missense mutations have facilitated researchers to understand the role of this key protein. Our study was aimed to evaluate the frequency of cytosine deletion in nonsmall cell lung cancer (NSCLC) patients.
  
  
  METHODS
  One hundred NSCLC patients were genotyped for P53 (exon5, codon168) cytosine deletion leading to loss of its function and activate the target genes by allele-specific polymerase chain reaction. The P53 cytosine deletion was correlated with all the clinicopathological parameters of the patients.
  
  
  RESULTS
  59% cases were carrying P53 cytosine deletion. Similarly, the significantly higher incidence of cytosine deletion was reported in current smokers (75%) in comparison to exsmoker and nonsmoker. Significantly higher frequency of cytosine deletion was reported in adenocarcinoma (68.08%) than squamous cell carcinoma (52.83%). Also, a significant difference was reported between p53 cytosine deletion and metastasis (64.28%). Further, the majority of the cases assessed for response carrying P53 cytosine deletion were found to show faster disease progression.
  
  
  CONCLUSIONS
  The data suggests that there is a significant association of the P53 exon 5 deletion of cytosine in codon 168 with metastasis and staging of the disease.
authors.affiliation: Prince Fahd Bin Sultan Research Chair Cancer Molecular Genetics, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Saudi Arabia, KSA.
authors.forename: Rashid
authors.initials: R
authors.lastname: Mir
authors.affiliation: Cancer Genetic Lab, Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: Mirza
authors.initials: M
authors.lastname: Masroor
authors.affiliation: Prince Fahd Bin Sultan Research Chair Cancer Molecular Genetics, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Saudi Arabia, KSA.
authors.forename: Jamsheed
authors.initials: J
authors.lastname: Javid
authors.affiliation: Cancer Genetic Lab, Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: Imtiyaz
authors.initials: I
authors.lastname: Ahamad
authors.affiliation: Cancer Genetic Lab, Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: Shazia
authors.initials: S
authors.lastname: Farooq
authors.affiliation: Cancer Genetic Lab, Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: Prasant
authors.initials: P
authors.lastname: Yadav
authors.affiliation: Cancer Genetic Lab, Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: Mariyam
authors.initials: M
authors.lastname: Zuberi
authors.affiliation: Department of Radiation oncology, SKIMS, Jammu and Kashmir, India.
authors.forename: Maqbool
authors.initials: M
authors.lastname: Lone
authors.affiliation: Cancer Genetic Lab, Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: P C
authors.initials: PC
authors.lastname: Ray
authors.affiliation: Cancer Genetic Lab, Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: Alpana
authors.initials: A
authors.lastname: Saxena
keywords: Nonsmall cell lung cancer; P53 (exon5, codon168) cytosine deletion; adeno-carcinoma; allele specific oligonucleotide; squamous cell carcinoma