title: Involvement of 15-lipoxygenase-1 in the regulation of breast cancer cell death induced by sodium butyrate.
abstract: 15-Lipoxygenase-1 (15-Lox-1) as a member of fatty acid dioxygenases family has received considerable attention as an effector of cancer cell growth. The relevance of sodium butyrate on 15-Lox-1 pathway has not been determined in breast cancer. This study is aimed to investigate the possible involvement of 15-Lox-1 in the regulation of breast cancer cell growth by sodium butyrate. MTT assay was used to assess the cytotoxicity effect and Annexin-V-FITC staining was applied for detection of apoptosis using flow cytometry. The involvement of 15-Lox-1 was examined using 15-Lox-1 specific inhibitor and enzyme gene expression level and activity was further analyzed by Real-time PCR and measurement of 13(S)-HODE. The results revealed that sodium butyrate increased the expression of 15-Lox-1 and production of 13(S)HODE. 15-Lox-1 was also involved in the sodium butyrate-induced breast cancer cell cytotoxicity and apoptosis. This study provided more evidences on the positive effectiveness of 15-Lox-1/13(S)-HODE on controlling growth of breast cancer cells.
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Salimi
authors.affiliation: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shabani
authors.affiliation: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.|Metabolic Disorders Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Nourbakhsh
authors.affiliation: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. tavakoli.m@iums.ac.ir.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Tavakoli-Yaraki
keywords: 15-Lipoxygenase-1; Apoptosis; Cell cytotoxicity; Sodium butyrate