title: An allograft mouse model for the study of hearing loss secondary to vestibular schwannoma growth.
abstract: Vestibular schwannoma is a benign neoplasm arising from the Schwann cell sheath of the auditory-vestibular nerve. It most commonly affects both sides in the genetic condition Neurofibromatosis type 2, causing progressive high frequency sensorineural hearing loss. Here, we describe a microsurgical technique and stereotactic coordinates for schwannoma cell grafting in the vestibular nerve region that recapitulates local tumor growth in the cerebellopontine angle and inner auditory canal with resulting hearing loss. Tumor growth was monitored by bioluminescence and MRI in vivo imaging, and hearing assessed by auditory brainstem responses. These techniques, by potentially enabling orthotopic grafting of a variety of cell lines will allow studies on the pathogenesis of tumor-related hearing loss and preclinical drug evaluation, including hearing endpoints, for NF2-related and sporadic schwannomas.
authors.affiliation: House Research Institute, Center for Neural Tumor Research, Los Angeles, CA, USA.|Department of Otology and Neurotology, University Hospital of Lille, and INSERM U1008 "Controlled Drug Delivery System and Biomaterials", University of Lille, Lille, France.
authors.forename: Nicolas-Xavier
authors.initials: NX
authors.lastname: Bonne
authors.affiliation: Department of Head and Neck Surgery, University of California Los Angeles, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.|Jonsson Comprehensive Cancer Center, University of California Los Angeles, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Vitte
authors.affiliation: House Research Institute, Center for Neural Tumor Research, Los Angeles, CA, USA.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chareyre
authors.affiliation: Department of Radiology, USC Keck School of Medicine, The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors.forename: Gevorg
authors.initials: G
authors.lastname: Karapetyan
authors.affiliation: Department of Radiology, USC Keck School of Medicine, The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors.forename: Vazgen
authors.initials: V
authors.lastname: Khankaldyyan
authors.affiliation: House Research Institute, Center for Neural Tumor Research, Los Angeles, CA, USA.
authors.forename: Karo
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Radiology, USC Keck School of Medicine, The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors.forename: Rex A
authors.initials: RA
authors.lastname: Moats
authors.affiliation: Department of Head and Neck Surgery, University of California Los Angeles, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. mgiovannini@mednet.ucla.edu.|Jonsson Comprehensive Cancer Center, University of California Los Angeles, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. mgiovannini@mednet.ucla.edu.
authors.forename: Marco
authors.initials: M
authors.lastname: Giovannini
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem; D034381:Hearing Loss / Q000209:etiology / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016518:Neurofibromatosis 2 / Q000235:genetics; D009464:Neuroma, Acoustic / Q000150:complications / Q000503:physiopathology*; D014184:Transplantation, Homologous; D000159:Vestibulocochlear Nerve / Q000601:surgery
keywords: Auditory brainstem response; Mouse model; Neurofibromatosis type 2; Vestibular schwannoma