title: Photoinduced apoptosis using a peptide carrying a photosensitizer.
abstract: A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.
authors.affiliation: Department of Medical Bioengineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Medical Bioengineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Fujiwara
authors.affiliation: Department of Applied Chemistry, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Kitamatsu
authors.affiliation: Department of Medical Bioengineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan. Electronic address: ohtsuk@okayama-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016466:CHO Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D055668:Photochemical Processes; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011806:Quinolinium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: C481052:Alexa fluor 546; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D010455:Peptides; D017319:Photosensitizing Agents; D011806:Quinolinium Compounds
keywords: Apoptosis; Bim; Cell-penetrating peptide; Photosensitizer