title: Development and validation of a point-of-care test for detecting hantavirus antibodies in human and rodent samples.
abstract: Hantaviruses are etiologic agents of a zoonotic disease transmitted mainly from wild rodents to humans, causing Hemorrhagic Fever with Renal Syndrome in Eurasia and the Hantavirus Cardiopulmonary Syndrome in the Americas (HCPS), reaching a lethality rate of 40% in Brazil. Hantavirus diagnostic and seroprevalence are often based on the presence of IgM and IgG antibodies against the virus. Here we propose a rapid test assay able to identify hantavirus antibodies with sensibility and specificity similar to ELISA assays. We analyzed five groups of samples, including healthy human population and small mammals of endemic areas, suspected cases of HCPS, patients with non-related infections and a serum panel from a different geographical region. The test presented good rates of sensibility (87-100%) and specificity (97-100%) for all groups, being a promising tool suitable for both rodent and human hantavirus epidemiological surveys.
authors.affiliation: Instituto Carlos Chagas-Fiocruz/PR, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba, PR, Brazil.
authors.forename: Andrea Cristine
authors.initials: AC
authors.lastname: Koishi
authors.affiliation: Instituto Carlos Chagas-Fiocruz/PR, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba, PR, Brazil.
authors.forename: Mateus Nóbrega
authors.initials: MN
authors.lastname: Aoki
authors.affiliation: Instituto Carlos Chagas-Fiocruz/PR, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba, PR, Brazil.
authors.forename: Taissa Ricciardi
authors.initials: TR
authors.lastname: Jorge
authors.affiliation: Instituto Carlos Chagas-Fiocruz/PR, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba, PR, Brazil.
authors.forename: Andréia Akemi
authors.initials: AA
authors.lastname: Suzukawa
authors.affiliation: Instituto Carlos Chagas-Fiocruz/PR, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba, PR, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Zanluca
authors.affiliation: Instituto Nacional de Enfermedades Virales Humanas Dr. J.I.Maiztegui, 2700, Pergamino, Argentina.
authors.forename: Silvana
authors.initials: S
authors.lastname: Levis
authors.affiliation: Instituto Carlos Chagas-Fiocruz/PR, Rua Prof. Algacyr Munhoz Mader, 3775, Curitiba, PR, Brazil. Electronic address: clsantos@fiocruz.br.
authors.forename: Claudia Nunes
authors.initials: CN
authors.lastname: Duarte Dos Santos
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006476:Orthohantavirus / Q000276:immunology*; D018778:Hantavirus Infections / Q000453:epidemiology* / Q000662:veterinary*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D019095:Point-of-Care Systems; D012377:Rodentia; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral
keywords: Hantavirus; Hantavirus cardiopulmonary syndrome (HCPS); Human seroprevalence; Rapid test; Rodents