title: Discovery of biomarkers for oxidative stress based on cellular metabolomics.
abstract: Oxidative stress has a close relationship with various pathologic physiology phenomena and the potential biomarkers of oxidative stress may provide evidence for clinical diagnosis or disease prevention. Metabolomics was employed to identify the potential biomarkers of oxidative stress. High-performance liquid chromatography-diode array detector, mass spectrometry and partial least squares discriminate analysis were used in this study. The 10, 15 and 13 metabolites were considered to discriminate the model group, vitamin E-treated group and l-glutathione-treated group, respectively. Some of them have been identified, namely, malic acid, vitamin C, reduced glutathione and tryptophan. Identification of other potential biomarkers should be conducted and their physiological significance also needs to be elaborated.
authors.affiliation: a Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University , Lanzhou , China ;|b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province , Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou , China ;|c Center of Resource Chemical & New Material , Qingdao , China.
authors.forename: Ningli
authors.initials: N
authors.lastname: Wang
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province , Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou , China ;|c Center of Resource Chemical & New Material , Qingdao , China.
authors.forename: Jianteng
authors.initials: J
authors.lastname: Wei
authors.affiliation: a Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University , Lanzhou , China ;
authors.forename: Yewei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province , Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou , China ;|c Center of Resource Chemical & New Material , Qingdao , China.
authors.forename: Dong
authors.initials: D
authors.lastname: Pei
authors.affiliation: a Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University , Lanzhou , China ;
authors.forename: Qingping
authors.initials: Q
authors.lastname: Hu
authors.affiliation: a Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University , Lanzhou , China ;
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province , Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou , China ;|c Center of Resource Chemical & New Material , Qingdao , China.
authors.forename: Duolong
authors.initials: D
authors.lastname: Di
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D016002:Discriminant Analysis; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D013058:Mass Spectrometry; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D018384:Oxidative Stress; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D014810:Vitamin E; D005978:Glutathione
keywords: Biomarkers; metabolomics; oxidative stress