title: Acylated oleanane-type triterpene saponins from the flowers of Bellis perennis show anti-proliferative activities against human digestive tract carcinoma cell lines.
abstract: Seven oleanane-type triterpene saponin bisdesmosides, perennisaponins N-T (1-7), were newly isolated from a methanol extract of daisy, the flowers of Bellis perennis L. (Asteraceae). The structures were determined based on chemical and physicochemical data and confirmed using previously isolated related compounds as references. The isolates, including 13 previously reported perennisaponins A-M (8-20), exhibited anti-proliferative activities against human digestive tract carcinoma HSC-2, HSC-4, and MKN-45 cells. Among them, perennisaponin O (2, IC50 = 11.2, 14.3, and 6.9 μM, respectively) showed relatively strong activities. The mechanism of action of 2 against HSC-2 was found to involve apoptotic cell death.
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.|Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ninomiya
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.|Koshiro Co., Ltd., 2-5-8 Doshomachi, Chuo-ku, Osaka, 541-0045, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Motai
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Nishida
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.|Koshiro Co., Ltd., 2-5-8 Doshomachi, Chuo-ku, Osaka, 541-0045, Japan.
authors.forename: Niichiro
authors.initials: N
authors.lastname: Kitagawa
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yoshihara
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hayakawa
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.|Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Muraoka
authors.affiliation: Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan.
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Li
authors.affiliation: Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan.
authors.forename: Seikou
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
authors.affiliation: Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan. matsuda@mb.kyoto-phu.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
authors.affiliation: Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan. morikawa@kindai.ac.jp.|Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan. morikawa@kindai.ac.jp.
authors.forename: Toshio
authors.initials: T
authors.lastname: Morikawa
mesh_terms: D045744:Cell Line, Tumor; D035264:Flowers / Q000737:chemistry*; D005770:Gastrointestinal Neoplasms / Q000235:genetics*; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D012503:Saponins / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry*
chemical_list: D012503:Saponins; D014315:Triterpenes; C413246:oleanane; D009828:Oleanolic Acid
keywords: Acylated oleanane-type triterpene saponin; Anti-proliferative activity; Apoptosis; Asteraceae; Bellis perennis; Daisy; Perennisaponin