title: Characterisation of fucoidan extracted from Malaysian Sargassum binderi.
abstract: Fucoidan is a sulphated polysaccharide that consists mainly of fucose, normally found in brown seaweeds. In this study, fucoidan was extracted from Sargassum binderi (Fsar) from Malaysia and subsequently characterised. The chemical characteristics of Fsar were found to be different than those of commercial food grade fucoidan (Fysk) and those of previously studied fucoidans. NMR analysis proposed that the main structure of Fsar is →3)fuc-2-OSO3(-)(1→3)fuc(1→. The molecular weight (47.87kDa) and degree of sulphation (0.20) of Fsar were higher than those of Fysk, at 27.98kDa and 0.15, respectively. However, Fsar's polydispersity index (1.12) and fucose content (34.50%) were lower than those of Fysk, at 1.88 and 43.30%, respectively. Both Fsar and Fysk showed similar thermo-gravimetric properties with four mass losses, amorphous in nature and negative optical rotations. Results show that Fsar has fundamental characteristics of fucoidan with different structural conformation i.e. variation in glycosidic linkages and sulphate group orientation.
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: joe@ukm.edu.my.
authors.forename: Seng Joe
authors.initials: SJ
authors.lastname: Lim
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: wanaidawm@ukm.edu.my.
authors.forename: Wan Mustapha
authors.initials: WM
authors.lastname: Wan Aida
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: maskatmy@gmail.com.
authors.forename: Mohamad Yusof
authors.initials: MY
authors.lastname: Maskat
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: jalifah@ukm.edu.my.
authors.forename: Jalifah
authors.initials: J
authors.lastname: Latip
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: kaybadri@ukm.edu.my.
authors.forename: Khairiah Haji
authors.initials: KH
authors.lastname: Badri
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: drosmanhassan@gmail.com.
authors.forename: Osman
authors.initials: O
authors.lastname: Hassan
authors.affiliation: School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: bohari@ukm.edu.my.
authors.forename: Bohari M
authors.initials: BM
authors.lastname: Yamin
mesh_terms: D005643:Fucose / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008296:Malaysia; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification*; D044647:Sargassum / Q000737:chemistry* / Q000254:growth & development; D012624:Seaweed / Q000737:chemistry* / Q000254:growth & development
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D005643:Fucose; C007789:fucoidan
keywords: Characterisation; Fucoidan; Sargassum binderi; Structure analysis