title: Supramolecular Ensembles Formed between Charged Conjugated Polymers and Glycoprobes for the Fluorogenic Recognition of Receptor Proteins.
abstract: This paper describes the simple construction of a unique class of supramolecular ensembles formed by electrostatic self-assembly between charged conjugated polymers and fluorophore-coupled glycoligands (glycoprobes) for the selective fluorogenic detection of receptor proteins at both the molecular and cellular levels. We show that positively and negatively charged diazobenzene-containing poly(p-phenylethynylenes) (PPEs) can be used to form stable fluorogenic probes with fluorescein-based (negatively charged) and rhodamine B based (positively charged) glycoprobes by electrostatic interaction. The structures of the ensembles have been characterized by spectroscopic and microscopic techniques. The supramolecular probes formed show quenched fluorescence in an aqueous buffer solution, which can be specifically recovered, in a concentration-dependent manner, through competitive complexation with a selective protein receptor, over a range of other unselective proteins. The ensembles also show selective fluorescence enhancement with a live cell that expresses the glycoligand receptor but not a control cell without receptor expression.
authors.affiliation: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, P. R. China.
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Dou
authors.affiliation: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, P. R. China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Zeng
authors.affiliation: The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University , Shenzhen 518055, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lv
authors.affiliation: The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University , Shenzhen 518055, P. R. China.
authors.forename: Jiatao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, P. R. China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: He
authors.affiliation: Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, P. R. China.
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Chen
authors.affiliation: The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University , Shenzhen 518055, P. R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Tan
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers; D011506:Proteins; D019793:Fluorescein
keywords: conjugated polymer; fluorescence; lectin; live cell; probe; receptor