title: The protective effect of North Schisandra Lignans on vascular endothelial cell oxidation injuries.
abstract: BACKGROUND
  In this study, the authors cultivated ECV-304 in vitro and incubated cells with H2O2, established injury models, and induced oxidized endothelial cell apoptosis. This model makes it possible to choose suitable concentrations of North Schisandra Lignans.
  
  
  OBJECTIVE
  To study the protective effects of North Schisandra Lignans on human umbilical vein endothelial cell injuries.
  
  
  METHODS
  Endothelial cell growth and proliferation activity were detected through the MTT method. The colorimetric method was used to determine superoxide dismutase (SOD) activity in the cell culture solution, as well as malondialdehyde (MDA) content in the cell.
  
  
  RESULTS
  North Schisandra Lignans noticeably decreased ECV-304 cell injury induced by H2O2. Moderate and high concentrations of North Schisandra Lignans could significantly lower MDA content and heighten SOD activity. These differences were significant compared to the H2O2 group (P< 0.05).
  
  
  CONCLUSIONS
  North Schisandra Lignans had an obvious protective effect on ECV-304 injured by H2O2$. The mechanism decreases MDA production and heightened SOD activity.
authors.affiliation: Beihua University Faculty of Medicine, Beihua University, Jilin, China.
authors.forename: Ruowen
authors.initials: R
authors.lastname: Zhang
authors.affiliation: The First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Jilin Gynecology and Obstetrics Hospital, Jilin, China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Dong
authors.affiliation: Pathogen Biology Department, College of Basic Medicine, Beihua University, Jilin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000473:pathology*; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D008315:Malondialdehyde; D018384:Oxidative Stress / Q000187:drug effects*; D032141:Schisandra
chemical_list: D000975:Antioxidants; D017705:Lignans; D008315:Malondialdehyde
keywords: North Schisandra Lignans; malondialdehyde; superoxide dismutase; vascular endothelial cell