title: Direct electrical and mechanical characterization of in situ generated DNA between the tips of silicon nanotweezers (SNT).
abstract: Previously, we reported the application of micromachined silicon nanotweezers (SNT) integrated with a comb-drive actuator and capacitive sensors for capturing and mechanical characterization of DNA bundles. Here, we demonstrate direct DNA amplification on such a MEMS structure with subsequent electrical and mechanical characterization of a single stranded DNA (ssDNA) bundle generated between the tips of SNT via isothermal rolling circle amplification (RCA) and dielectrophoresis (DEP). An in situ generated ssDNA bundle was visualized and evaluated via electrical conductivity (I-V) and mechanical frequency response measurements. Colloidal gold nanoparticles significantly enhanced (P < 0.01) the electrical properties of thin ssDNA bundles. The proposed technology allows direct in situ synthesis of DNA with a predefined sequence on the tips of a MEMS sensor device, such as SNT, followed by direct DNA electrical and mechanical characterization. In addition, our data provides a "proof-of-principle" for the feasibility of the on-chip label free DNA detection device that can be used for a variety of biomedical applications focused on sequence specific DNA detection.
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. momo@iis.u-tokyo.ac.jp and NeuroInDx. Inc., 20725 S. Western Ave., Suite 100, Torrance, CA 90501, USA. skarsten@neuroindx.com.
authors.forename: Stanislav L
authors.initials: SL
authors.lastname: Karsten
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. momo@iis.u-tokyo.ac.jp.
authors.forename: Momoko
authors.initials: M
authors.lastname: Kumemura
authors.affiliation: LIMMS/CNRS-IIS (UMI 2820), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Laurent
authors.initials: L
authors.lastname: Jalabert
authors.affiliation: LIMMS/CNRS-IIS (UMI 2820), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lafitte
authors.affiliation: NeuroInDx. Inc., 20725 S. Western Ave., Suite 100, Torrance, CA 90501, USA. skarsten@neuroindx.com.
authors.forename: Lili C
authors.initials: LC
authors.lastname: Kudo
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. momo@iis.u-tokyo.ac.jp and LIMMS/CNRS-IIS (UMI 2820), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan and SMMiL-E: Institut pour la Recherche sur le Cancer de Lille, Boulevard du Pr Jules Leclercq, 59 000 Lille, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Collard
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. momo@iis.u-tokyo.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fujita
mesh_terms: D004277:DNA, Single-Stranded / Q000235:genetics*; D004560:Electricity; D055595:Mechanical Phenomena; D036103:Nanotechnology / Q000295:instrumentation*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D012825:Silicon
chemical_list: D004277:DNA, Single-Stranded; D012825:Silicon