title: Mass Spectrometry for Paper-Based Immunoassays: Toward On-Demand Diagnosis.
abstract: Current analytical methods, either point-of-care or centralized detection, are not able to meet recent demands of patient-friendly testing and increased reliability of results. Here, we describe a two-point separation on-demand diagnostic strategy based on a paper-based mass spectrometry immunoassay platform that adopts stable and cleavable ionic probes as mass reporter; these probes make possible sensitive, interruptible, storable, and restorable on-demand detection. In addition, a new touch paper spray method was developed for on-chip, sensitive, and cost-effective analyte detection. This concept is successfully demonstrated via (i) the detection of Plasmodium falciparum histidine-rich protein 2 antigen and (ii) multiplexed and simultaneous detection of cancer antigen 125 and carcinoembryonic antigen.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States.
authors.forename: Suming
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States.
authors.forename: Qiongqiong
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States.
authors.forename: Abraham K
authors.initials: AK
authors.lastname: Badu-Tawiah
mesh_terms: D002272:Carcinoembryonic Antigen / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D016778:Malaria, Falciparum / Q000175:diagnosis; D013058:Mass Spectrometry / Q000379:methods*; D010209:Paper; D010963:Plasmodium falciparum / Q000737:chemistry; D011506:Proteins / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000099053:Histidine-Rich Glycoprotein
chemical_list: D002272:Carcinoembryonic Antigen; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein