title: Correlating microscopy techniques and ToF-SIMS analysis of fully grown mammalian oocytes.
abstract: The 2D-molecular thin film analysis protocol for fully grown mice oocytes is described using an innovative approach. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and optical microscopy imaging were applied to the same mice oocyte section on the same sample holder. A freeze-dried mice oocyte was infiltrated into embedding media, e.g. Epon, and then was cut with a microtome and 2 μm thick sections were transferred onto an ITO coated conductive glass. Mammalian oocytes can contain "nucleolus-like body" (NLB) units and ToF-SIMS analysis was used to investigate the NLB composition. The ion-spatial distribution in the cell components was identified and compared with the images acquired by SEM, AFM and optical microscopy. This study presents a significant advancement in cell embryology, cell physiology and cancer-cell biochemistry.
authors.affiliation: N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119991 Moscow, Russia. nadtochenko@gmail.com and Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russian Federation.
authors.forename: Alexander
authors.initials: A
authors.lastname: Gulin
authors.affiliation: N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119991 Moscow, Russia. nadtochenko@gmail.com and Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russian Federation and Institute of Problems of Chemical Physics, Russian Academy of Sciences, pr. Akademika Semenova 1, 142432 Chernogolovka, Moscow Region, Russia.
authors.forename: Victor
authors.initials: V
authors.lastname: Nadtochenko
authors.affiliation: N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119991 Moscow, Russia. nadtochenko@gmail.com.
authors.forename: Artyom
authors.initials: A
authors.lastname: Astafiev
authors.affiliation: Institute of Theoretical and Experimental Biophysics, RAS, ul. Institustskaya 3, 142290 Pushchino, Moscow Region, Russia.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pogorelova
authors.affiliation: Ecole Polytechnique Fédeérale de Lausanne, Institute of chemical sciences and engineering (ISIC), Lausanne, VD, CH 1015, Switzerland.
authors.forename: Sami
authors.initials: S
authors.lastname: Rtimi
authors.affiliation: Institute of Theoretical and Experimental Biophysics, RAS, ul. Institustskaya 3, 142290 Pushchino, Moscow Region, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pogorelov
mesh_terms: D000818:Animals; D005612:Freeze Drying; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D009865:Oocytes / Q000166:cytology*; D018629:Spectrometry, Mass, Secondary Ion