title: A bioengineered drug-Eluting scaffold accelerated cutaneous wound healing In diabetic mice.
abstract: Hyperglycemia in diabetic patients can greatly hinder the wound healing process. In this study we investigated if the engagement of F4/80(+) murine macrophages could accelerate the cutaneous wound healing in streptozotocin induced diabetic mice. To facilitate the engagement of macrophages, we engineered a drug-eluting electrospun scaffold with a payload of monocyte chemoattractant protein-1 (MCP-1). MCP-1 could be readily released from the scaffold within 3 days. The electrospun scaffold showed no cytotoxic effects on human keratinocytes in vitro. Full-thickness excisional cutaneous wound was created in diabetic mice. The wound fully recovered within 10 days in mice treated with the drug-eluting scaffold. In contrast, the wound took 14 days to fully recover in control groups. The use of drug-eluting scaffold also improved the re-epithelialization. Furthermore, we observed a larger population of F4/80(+) macrophages in the wound bed of mice treated with drug-eluting scaffolds on day 3. This marked increase of macrophages in the wound bed could have contributed to the accelerated wound healing. Our study shed new light on an immuno-engineering solution for wound healing management in diabetic patients.
authors.affiliation: Organ Transplant Center, Shanghai Changzheng Hospital, Shanghai 200003, China; Department of Surgery, The University of Chicago, Chicago, IL 60637, United States.
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
authors.affiliation: Organ Transplant Center, Shanghai Changzheng Hospital, Shanghai 200003, China.
authors.forename: Guoshan
authors.initials: G
authors.lastname: Ding
authors.affiliation: Organ Transplant Center, Shanghai Changzheng Hospital, Shanghai 200003, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Shi
authors.affiliation: Organ Transplant Center, Shanghai Changzheng Hospital, Shanghai 200003, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Organ Transplant Center, Shanghai Changzheng Hospital, Shanghai 200003, China.
authors.forename: Zhijia
authors.initials: Z
authors.lastname: Ni
authors.affiliation: Organ Transplant Center, Shanghai Changzheng Hospital, Shanghai 200003, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Fu
authors.affiliation: Organ Transplant Center, Shanghai Changzheng Hospital, Shanghai 200003, China. Electronic address: zhirenfu.sh@gmail.com.
authors.forename: Zhiren
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000818:Animals; D057005:Bioengineering / Q000379:methods*; D002417:Cattle; D018932:Chemokine CCL2 / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008810:Mice, Inbred C57BL; D012867:Skin / Q000187:drug effects / Q000473:pathology; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D018932:Chemokine CCL2
keywords: Diabetic wound healing; Electrospinning; Monocyte chemoattractant protein-1; Tissue engineering