title: Tabebuia roseoalba: In Vivo Hypouricemic and Anti-inflammatory Effects of Its Ethanolic Extract and Constituents.
abstract: Tabebuiaspecies have several uses in folk medicine, including their use to treat inflammation and rheumatism. The aim of this study was to investigate whether the ethanolic extract of leaves fromTabebuia roseoalbaand isolated compounds could be useful to decrease serum uric acid levels and restrain the gout inflammatory process. The compoundsα-amyrin,β-amyrin, sitosterol, and stigmasterol were isolated from the ethanolic extract. Rutin and caffeic acid were identified in the ethanolic extract by HPLC analysis. The anti-hyperuricemic effect, liver xanthine oxidoreductase inhibition, and anti-inflammatory activity of the ethanolic extract and isolated compounds were evaluated on hyperuricemic mice and on paw edema induced by monosodium urate crystals in mice. The ethanolic extract of leaves fromT. roseoalba, β-amyrin, and stigmasterol were able to reduce serum uric acid levels in hyperuricemic mice through inhibition of liver xanthine oxidase activity and significantly decreased the paw edema induced by monosodium urate crystals. The antioxidant activity of the ethanolic extract and its ability to inhibit xanthine oxidase were also evaluatedin vitro. The ethanolic extract of leaves fromT. roseoalbashowed significant antioxidant activity in the three evaluated assays. Results were analyzed using GraphPad Prism 5.01. One-way ANOVA followed by Student's Newman-Keul's test was used to determine the significant differences between groups. The results show that the ethanolic extract of leaves fromT. roseoalba, β-amyrin, and stigmasterol can be promising agents for the treatment for gouty arthritis, hyperuricemia, and inflammation. Stigmasterol,β-amyrin, and rutin contribute to the observed effects of the ethanolic extract of leaves fromT. roseoalba.
authors.affiliation: Departamento de Química, Instituto Federal de Minas Gerais-Campus Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Zilma Schimith
authors.initials: ZS
authors.lastname: Ferraz-Filha
authors.affiliation: Laboratório de Plantas Medicinais, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Marcela Carolina de Paula
authors.initials: MC
authors.lastname: Michel Araújo
authors.affiliation: Laboratório de Plantas Medicinais, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Fernanda Cristina
authors.initials: FC
authors.lastname: Ferrari
authors.affiliation: Laboratório de Plantas Medicinais, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Iális Paiva Andrade Rodrigues
authors.initials: IP
authors.lastname: Dutra
authors.affiliation: Laboratório de Plantas Medicinais, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
authors.forename: Dênia Antunes
authors.initials: DA
authors.lastname: Saúde-Guimarães
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis; D000975:Antioxidants / Q000032:analysis; D015210:Arthritis, Gouty / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D006074:Gout Suppressants / Q000032:analysis; D033461:Hyperuricemia / Q000188:drug therapy*; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D029663:Tabebuia / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D006074:Gout Suppressants; D010936:Plant Extracts