title: [Study of pharmacokinetics of digoxin in ovariectomized rats model].
abstract: This study aims to investigate the change of plasma concentration of digoxin (DIG) in rats with ovariectomy. Twelve female SD rats were randomly assigned into ovariectomized group and sham group (n = 6). All rats plasma was collected after a single dose of 2 mg x kg(-1) DIG administrated orally, serum DIG concentration was determined by LC-MS/MS. The level of P-gp in the intestinal was analyzed by Western blotting. Pharmacokinetic calculations were performed on each individual using DAS 2.0 practical pharmacokinetic software. Compared with the sham group, C(max) of ovariectomized group decreased significantly (P < 0.01). There was no significant difference of AUC(0-t), and the level of P-gp was elevated in ovariectomized group. It was found that C(max) of DIG was significantly reduced after ovariectomy, and the change was associated with the decreased level of estrogen, which contributes to the increased level of P-gp.
authors.forename: Yong-wen
authors.initials: YW
authors.lastname: Jin
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Qin
authors.forename: Zhi
authors.initials: Z
authors.lastname: Rao
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Zhang
authors.forename: Yan Rong
authors.initials: YR
authors.lastname: Ma
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Wei
authors.forename: Xin-an
authors.initials: XA
authors.lastname: Wu
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004077:Digoxin / Q000097:blood* / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D004967:Estrogens / Q000097:blood; D005260:Female; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004967:Estrogens; D004077:Digoxin