title: Salbutamol Residues in Plasma, Urine and Hair of Heifers After a Single Dose and Throughout.
abstract: The objective of this study was to evaluate the salbutamol residues in the plasma, urine and hair of heifers after a single dose. Three heifers were given a single oral dose of salbutamol hydrochloride (150 μg/kg bodyweight). The salbutamol concentrations were measured in the plasma, urine (before and after hydrolysis with β-glucuronidase/arylsulfatase) and hair samples with ultra-performance liquid chromatography-tandem mass spectrometry. In the unhydrolyzed samples, the peak concentrations of salbutamol occurred in the plasma and urine at 12 and 8 h after drug administration, respectively, but were below the limit of quantification (LOQ = 0.2 ng/mL) at 48 and 120 h after administration, respectively. However, in the hydrolyzed samples, the salbutamol concentration was 1.1 ng/mL in the plasma 72 h after its administration and 0.7 ng/mL in the urine 168 h after its administration. Thus, the concentrations of salbutamol were significantly higher in the hydrolyzed samples than that in the unhydrolyzed samples (P < 0.01). The concentrations of salbutamol in the black and white hair 24 h after its administration were 1.7 and 1.0 ng/g, respectively. These results indicate that hair may be a target tissue for detecting the misuse of salbutamol after a single dose and that the primary forms of salbutamol in the plasma and urine samples from heifers are its sulfate and glucuronide conjugates.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, NO. 2 of Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, NO. 2 of Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Liang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, NO. 2 of Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
authors.forename: Chuanyou
authors.initials: C
authors.lastname: Su
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, NO. 2 of Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
authors.forename: Chaohua
authors.initials: C
authors.lastname: Tang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, NO. 2 of Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, NO. 2 of Yuanmingyuan West Road, Haidian District, Beijing 100193, China zhjmxms@sina.com.
authors.forename: Junmin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China, Ministry of Agriculture, NO. 2 of Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
authors.forename: Qingshi
authors.initials: Q
authors.lastname: Meng
mesh_terms: D000420:Albuterol / Q000097:blood / Q000378:metabolism* / Q000652:urine; D000818:Animals; D001993:Bronchodilator Agents / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002417:Cattle; D005260:Female; D006197:Hair / Q000378:metabolism; D010949:Plasma / Q000378:metabolism; D014556:Urine
chemical_list: D001993:Bronchodilator Agents; D000420:Albuterol