title: Evaluation of the role of the GPIb-IX-V receptor complex in development of the platelet storage lesion.
abstract: OBJECTIVE
  In mice, loss of sialic acid resulting in shedding of glycoprotein (GP) Ibα and GPV has been linked to platelet survival. The aim of this study was to determine whether loss of sialic acid and the GPIb-IX-V complex contributes to development of the platelet storage lesion (PSL) in human platelet concentrates (PCs).
  
  
  METHODS
  PCs (stored in plasma (with or without Mirasol treatment); PAS-C or PAS-E) were stored at room temperature. Flow cytometry was used to monitor membrane expression of the GPIb-IX-V complex, CD62P, surface glycans and PS exposure. The functionality of stored platelets was determined employing aggregometry and ristocetin-induced VWF binding.
  
  
  RESULTS
  Storage time of PCs in blood banks is limited to 7 days. During this time period, a minor but gradually increasing subpopulation of GPIbα-negative platelets was observed. Also, ristocetin-induced VWF binding was impaired in a small population of platelets. Mean surface expression of GPIbα and GPV remained stable until day 9, whereas CD62P expression increased; also a rapid decrease in ADP-induced aggregation was observed for PAS-C, PAS-E and Mirasol-treated PCs. Upon prolonged storage (>9 days), a slow decline in surface expression of GPIbα and GPV was observed; no major changes were observed in surface sialylation with the exception of Mirasol-treated platelets.
  
  
  CONCLUSIONS
  In a small population of stored platelets, changes in GPIbα occur from day 2 onwards. Loss of sialic acid and subsequent shedding of GPIbα and GPV is not an early event during the development of the PSL.
authors.affiliation: Department of Plasma Proteins, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Rijkers
authors.affiliation: Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands.
authors.forename: P F
authors.initials: PF
authors.lastname: van der Meer
authors.affiliation: Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands.
authors.forename: I J
authors.initials: IJ
authors.lastname: Bontekoe
authors.affiliation: Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands.
authors.forename: B B
authors.initials: BB
authors.lastname: Daal
authors.affiliation: Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands.|Department of Blood Cell Research, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: de Korte
authors.affiliation: Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: F W G
authors.initials: FW
authors.lastname: Leebeek
authors.affiliation: Department of Plasma Proteins, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands. j.voorberg@sanquin.nl.
authors.forename: J
authors.initials: J
authors.lastname: Voorberg
authors.affiliation: Department of Plasma Proteins, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands.|Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: A J G
authors.initials: AJ
authors.lastname: Jansen
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001793:Blood Preservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D011485:Protein Binding; D012310:Ristocetin / Q000494:pharmacology; D014841:von Willebrand Factor / Q000737:chemistry / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D019007:P-Selectin; D019038:Platelet Glycoprotein GPIb-IX Complex; C032193:glycoprotein receptor GPIb-IX; D014841:von Willebrand Factor; D012310:Ristocetin; D019158:N-Acetylneuraminic Acid
keywords: aggregation; glycoprotein Ibα; mirasol; platelet storage lesion; sialic acid