title: The TcTASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs.
abstract: In regions where Chagas disease is endemic, canine Trypanosoma cruzi infection is highly correlated with the risk of transmission of the parasite to humans. Herein we evaluated the novel TcTASV protein family (subfamilies A, B, C), differentially expressed in bloodstream trypomastigotes, for the detection of naturally infected dogs. A gene of each TcTASV subfamily was cloned and expressed. Indirect enzyme-linked immunosorbent assays (ELISA) were developed using recombinant antigens individually or mixed together. Our results showed that dogs with active T. cruzi infection differentially reacted against the TcTASV-C subfamily. The use of both TcTASV-C plus TcTASV-A proteins (Mix A+C-ELISA) enhanced the reactivity of sera from dogs with active infection, detecting 94% of the evaluated samples. These findings agree with our previous observations, where the infected animals exhibited a quick anti-TcTASV-C antibody response, coincident with the beginning of parasitaemia, in a murine model of the disease. Results obtained in the present work prove that the Mix A+C-ELISA is a specific, simple and cheap technique to be applied in endemic areas in screening studies. The Mix A+C-ELISA could help to differentially detect canine hosts with active infection and therefore with high impact in the risk of transmission to humans.
authors.affiliation: Cátedra de Química Biológica,Facultad de Ciencias Naturales,Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Floridia-Yapur
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Monje Rumi
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Ragone
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lauthier
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Tomasini
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Alberti D'Amato
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Diosque
authors.affiliation: Cátedra de Química Biológica,Facultad de Ciencias Naturales,Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Cimino
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: J D
authors.initials: JD
authors.lastname: Marco
authors.affiliation: Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Barroso
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo A. Ugalde" (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET,Av. 25 de Mayo,Partido de San Martín,Buenos Aires (1650),Argentina.
authors.forename: D O
authors.initials: DO
authors.lastname: Sanchez
authors.affiliation: Cátedra de Química Biológica,Facultad de Ciencias Naturales,Universidad Nacional de Salta,Av. Bolivia 5150,Salta (4400),Argentina.
authors.forename: J R
authors.initials: JR
authors.lastname: Nasser
authors.affiliation: Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo A. Ugalde" (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET,Av. 25 de Mayo,Partido de San Martín,Buenos Aires (1650),Argentina.
authors.forename: V
authors.initials: V
authors.lastname: Tekiel
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D014355:Chagas Disease / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods / Q000662:veterinary; D051379:Mice; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan
keywords: TcTASV antigens; Trypanosoma cruzi; dogs; immunodiagnosis