title: MicroRNA-7 inhibits neuronal apoptosis in a cellular Parkinson's disease model by targeting Bax and Sirt2.
abstract: Parkinson's disease (PD) is the second most common age-related neurodegenerative disease. MicroRNA-7 (miR-7) displays neuroprotective properties against PD. However, the biological roles of miR-7 and its underlying molecular mechanisms in PD remain unclear. We demonstrated herein that 1-methyl-4-phenylpyridinium ion (MPP(+)) confers toxic effects on dopaminergic neuron in a dose-dependent manner in a cellular PD model, although this phenomenon is attenuated by miR-7 treatment. Introduction of miR-7 inhibits MPP(+)-induced neuronal apoptosis as reflected by the reduced terminal transferase-mediated dUTP nick end labeling-positive rate, mitochondrial permeability potential, caspase 3 activity, and nucleosomal enrichment factor. Bax and sirtuin 2 (Sirt2) are the direct targets of miR-7. Moreover, the effects of miR-7 were counteracted by Bax and Sirt2 overexpression, respectively. The altered molecular expressions downstream of Bax and Sirt2 are also involved in miR-7 regulation of the MPP(+)-triggered neuronal apoptosis. These findings have implications on the potential application of miR-7 in PD treatment.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou 450014, China.
authors.forename: Shize
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Pharmacy, The First People's Hospital of Shangqiu 476100, Henan, China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou 450014, China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Zhai
authors.affiliation: Department of Neurology, Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou 450007, China.
authors.forename: Ruyan
authors.initials: R
authors.lastname: Xu
authors.affiliation: Department of Neurology, Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou 450007, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou 450007, China.
authors.forename: Songyao
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou 450007, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Neurology, Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou 450007, China.
authors.forename: Liujie
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Zhengzhou University Zhengzhou 450014, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Lou
keywords: Bax; MicroRNA-7; Parkinson’s disease; Sirt2; apoptosis