title: Impaired NK cells' activity and increased numbers of CD4 + CD25+ regulatory T cells in multidrug-resistant Mycobacterium tuberculosis patients.
abstract: Multidrug-resistant tuberculosis (MDR-TB) often causes persistent infection and chemotherapy failure, which brings heavy burden of society and family. Many immune cell subsets and regulatory mechanisms may operate throughout the various stages of infection. The presence of regulatory T cells (Tregs) is thought to be an important mechanism that TB successfully evades the immune system. Tregs play a central role in the prevention of autoimmunity and in the control of immune responses. The role of Tregs in MDR-TB infection and persistence is inadequately documented. The current study was designed to determine whether CD4 + CD25+ regulatory T cells may modulate innate immunity (such as NK cells) against human tuberculosis. Our results indicated that the numbers of CD4 + CD25+ Treg cells increased in MDR-TB patients' blood, and the cytokine production of IL-10 increased from MDR-patients compared with healthy subjects, along with the lower activity and low CD69 expression of NK cells in patients. These results suggested that immunity to MDR-TB patients induced circulating CD4 + CD25+ T regulatory cells expansion, which may be related to the persistence of Mycobacterium tuberculosis (M. tb) infection, and to the balance between effectors immune responses and suppression immune responses.
authors.affiliation: Changsha Central Hospital, China. Electronic address: fanrenhua04@sina.com.
authors.forename: Renhua
authors.initials: R
authors.lastname: Fan
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Yangen
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Changsha Central Hospital, China. Electronic address: yangli@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Yanke
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Southeast University Medicine School, China.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Feng
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yin
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Manjiao
authors.initials: M
authors.lastname: Fu
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Changsha Central Hospital, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002450:Cell Communication; D049109:Cell Proliferation; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood / Q000276:immunology*; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D037181:Lectins, C-Type / Q000097:blood / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000472:pathogenicity; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D055815:Young Adult; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D016753:Interleukin-10; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C508609:IL10 protein, human; C508017:IL2RA protein, human
keywords: CD4 + CD25+ regulatory T cells; CD69; Multidrug-resistant tuberculosis; NK cells' activity