title: Methods to study the coupling between replicative helicase and leading-strand DNA polymerase at the replication fork.
abstract: Replicative helicases work closely with the replicative DNA polymerases to ensure that the genomic DNA is copied in a timely and error free manner. In the replisomes of prokaryotes, mitochondria, and eukaryotes, the helicase and DNA polymerase enzymes are functionally and physically coupled at the leading strand replication fork and rely on each other for optimal DNA strand separation and synthesis activities. In this review, we describe pre-steady state kinetic methods to quantify the base pair unwinding-synthesis rate constant, a fundamental parameter to understand how the helicase and polymerase help each other during leading strand replication. We describe a robust method to measure the chemical step size of the helicase-polymerase complex that determines how the two motors are energetically coupled while tracking along the DNA. The 2-aminopurine fluorescence-based method provide structural information on the leading strand helicase-polymerase complex, such as the distance between the two enzymes, their relative positions at the replication fork, and their roles in fork junction melting. The combined information garnered from these methods informs on the mutual dependencies between the helicase and DNA polymerase enzymes, their stepping mechanism, and their individual functions at the replication fork during leading strand replication.
authors.affiliation: Department of Biochemistry and Molecular Biology, Rutgers, Robert Wood Johnson Medical School, 683 Hoes Lane West, Piscataway 08854, NJ, USA.
authors.forename: Divya
authors.initials: D
authors.lastname: Nandakumar
authors.affiliation: Department of Biochemistry and Molecular Biology, Rutgers, Robert Wood Johnson Medical School, 683 Hoes Lane West, Piscataway 08854, NJ, USA. Electronic address: patelss@rutgers.edu.
authors.forename: Smita S
authors.initials: SS
authors.lastname: Patel
mesh_terms: D015075:2-Aminopurine / Q000737:chemistry; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D007700:Kinetics; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation
chemical_list: D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D015075:2-Aminopurine; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases
keywords: Bacteriophage T7 replication; Base pair unwinding-synthesis rate constant; Chemical step size; DNA polymerase; Mitochondrial DNA replication; Pre-steady state kinetics; Replicative helicase; Structural biology