title: Development of a DNA-Templated Peptide Probe for Photoaffinity Labeling and Enrichment of the Histone Modification Reader Proteins.
abstract: Histone post-translational modifications (HPTMs) provide signal platforms to recruit proteins or protein complexes to regulate gene expression. Therefore, the identification of these recruited partners (readers) is essential to understand the underlying regulatory mechanisms. However, it is still a major challenge to profile these partners because their interactions with HPTMs are rather weak and highly dynamic. Herein we report the development of a HPTM dual probe based on DNA-templated technology and a photo-crosslinking method for the identification of HPTM readers. By using the trimethylation of histone H3 lysine 4, we demonstrated that this HPTM dual probe can be successfully utilized for labeling and enrichment of HPTM readers, as well as for the discovery of potential HPTM partners. This study describes the development of a new chemical proteomics tool for profiling HPTM readers and can be adapted for broad biomedical applications.
authors.affiliation: Department of Chemistry, Nankai University, Weijin Road 94#, Tianjin, 300071, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Chemistry, Nankai University, Weijin Road 94#, Tianjin, 300071, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Lu
authors.affiliation: College of Pharmacy, Nankai University, Weijin Road 94#, Tianjin, 300071, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Jin
authors.affiliation: Tianjin Key Laboratory of Medical Epigenetics, 2011 Collaborative Innovation Center of Tianjin for Medical, Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Qixiangtai Road 22#, 300070, Tianjin, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Tian
authors.affiliation: Tianjin Key Laboratory of Medical Epigenetics, 2011 Collaborative Innovation Center of Tianjin for Medical, Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Qixiangtai Road 22#, 300070, Tianjin, China.
authors.forename: Zhenchang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Tianjin Key Laboratory of Medical Epigenetics, 2011 Collaborative Innovation Center of Tianjin for Medical, Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Qixiangtai Road 22#, 300070, Tianjin, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Chen
authors.affiliation: Tianjin Key Laboratory of Medical Epigenetics, 2011 Collaborative Innovation Center of Tianjin for Medical, Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Qixiangtai Road 22#, 300070, Tianjin, China.
authors.forename: Guijin
authors.initials: G
authors.lastname: Zhai
authors.affiliation: Department of Chemistry, Nankai University, Weijin Road 94#, Tianjin, 300071, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, Nankai University, Weijin Road 94#, Tianjin, 300071, China.
authors.forename: Xiwen
authors.initials: X
authors.lastname: He
authors.affiliation: Institut für Biochemie und Molekularbiologie, Universität Freiburg, Stefan-Meier-Straße 17, Freiburg, 79104, Germany.
authors.forename: Enguo
authors.initials: E
authors.lastname: Fan
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Medical Epigenetics, 2011 Collaborative Innovation Center of Tianjin for Medical, Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Qixiangtai Road 22#, 300070, Tianjin, China. kzhang@tmu.edu.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
mesh_terms: D004247:DNA / Q000737:chemistry*; D006367:HeLa Cells; D042421:Histone Code; D006657:Histones / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000032:analysis; D008745:Methylation; D010455:Peptides / Q000737:chemistry*; D019920:Photoaffinity Labels / Q000737:chemistry*; D055668:Photochemical Processes; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods
chemical_list: D006657:Histones; D010455:Peptides; D019920:Photoaffinity Labels; D004247:DNA; D008239:Lysine
keywords: epigenetics; histones; photo-crosslinking; protein modifications; proteomics