title: Assessment of platelet function in healthy cats in response to commonly prescribed antiplatelet drugs using three point-of-care platelet function tests.
abstract: Objectives The objective was to determine if decreased platelet function could be detected after treatment with aspirin and/or clopidogrel in healthy cats using three point-of-care platelet function tests that evaluate platelet function by different methods: Multiplate (by impedance), Platelet Function Analyzer 100 (by mechanical aperture closure) and Plateletworks (by platelet counting). Methods Thirty-six healthy cats were randomly assigned to receive one of three oral treatments over an 8 day period: (1) aspirin 5 mg q72h; (2) aspirin 20.25 mg q72h; or (3) clopidogrel 18.75 mg q24h. Cats treated with 5 and 20.25 mg aspirin also received clopidogrel on days 4-8. Platelet aggregation in response to adenosine diphosphate and collagen ± arachidonic acid was assessed on days 1 (baseline), 4 and 8. Aspirin and clopidogrel metabolites were measured by high-performance liquid chromatography. Platelet function in response to treatment was analyzed by ANCOVA, linear regression and Spearman correlation. Results The only solitary aspirin effect was detected using Plateletworks with collagen in cats treated with 20.25 mg. The only effect detected by Multiplate was using arachidonic acid in cats treated with both aspirin 20.25 mg and clopidogrel. All clopidogrel treatment effects were detected by Platelet Function Analyzer 100, Plateletworks (adenosine diphosphate) and Plateletworks (collagen). Drug metabolites were present in all cats, but concentrations were minimally correlated to platelet function test results. Conclusions and relevance Platelet Function Analyzer 100 and Plateletworks using adenosine diphosphate ± collagen agonists may be used to detect decreased platelet function in response to clopidogrel treatment. Either aspirin is not as effective an antiplatelet drug as clopidogrel, or the tests used were not optimal to measure aspirin effect. Cats with heart disease are commonly prescribed antiplatelet drugs to decrease the risk of aortic thromboembolism. Platelet Function Analyzer 100 and Plateletworks may be useful for confirming clopidogrel treatment in these cats.
authors.affiliation: 1 Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Kimberly K
authors.initials: KK
authors.lastname: Ho
authors.affiliation: 1 Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Anthony Cg
authors.initials: AC
authors.lastname: Abrams-Ogg
authors.affiliation: 2 Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: R Darren
authors.initials: RD
authors.lastname: Wood
authors.affiliation: 1 Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: M Lynne
authors.initials: ML
authors.lastname: O'Sullivan
authors.affiliation: 3 Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Gordon M
authors.initials: GM
authors.lastname: Kirby
authors.affiliation: 1 Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Shauna L
authors.initials: SL
authors.lastname: Blois
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology*; D001780:Blood Coagulation Tests / Q000662:veterinary; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002415:Cats / Q000097:blood*; D000077144:Clopidogrel; D005260:Female; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D010979:Platelet Function Tests / Q000662:veterinary; D019095:Point-of-Care Systems; D013988:Ticlopidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D010975:Platelet Aggregation Inhibitors; D000077144:Clopidogrel; D013988:Ticlopidine; D001241:Aspirin