title: Selective and sensitive detection of MiRNA-21 based on gold-nanorod functionalized polydiacetylene microtube waveguide.
abstract: Development of rapid, highly selective and sensitive miRNA detection in a complex biological environment has attracted considerable attention. Herein, we describe a novel two step method to construct gold-nanorod functionalized polydiacetylene (PDA) microtube for miRNA detection. In PDA microtube, with a one-dimensional (1D) waveguide nature, the excitation position and emission out-coupling position are far apart, thus helpful in reducing contribution of auto-fluorescence from biological sample. The use of specially designed toehold-mediated strand displacement reaction enables the reliable and selective discrimination of miRNA sequences with high sequence homology. Based on the condensing enrichment effect, the detection limit of the proposed PDA microtube system is as low as 0.01nM, and it can be applied directly to detect disease-specific miRNA targets in human serum. This PDA microtube waveguide system can be further integrated into the chip for the potential applications in minimally invasive, portable clinical diagnostic equipment.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Qiu
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
authors.forename: Mengqiao
authors.initials: M
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China.
authors.forename: Qijin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
authors.forename: Pei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Ming
authors.affiliation: Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, PR China. Electronic address: dgzhang@ustc.edu.cn.
authors.forename: Douguo
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Division of Gastroenterology, Affiliated Provincial Hospital, Anhui Medical University, No.17 Lu Jiang Road, Hefei, Anhui 230001, PR China. Electronic address: yuyuemd@yahoo.com.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yu
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, iChEM, University of Science and Technology of China, Hefei, Anhui 230026 PR China. Electronic address: gangzou@ustc.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Zou
authors.affiliation: Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States.
authors.forename: Ramachandram
authors.initials: R
authors.lastname: Badugu
authors.affiliation: Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Lakowicz
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis / Q000097:blood*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D006046:Gold
keywords: Biosensing; MicroRNA; Optical waveguide; Polydiacetylene