title: Effects of monomethoxypolyethylene glycol-chitosan nanoparticle-mediated dual silencing of livin and survivin genes in prostate cancer PC-3M cells.
abstract: Monomethoxypolyethylene glycol-chitosan (mPEG-CS) nanoparticles were used as interfering RNA carriers to transfect human prostate cancer PC-3M cells to evaluate the effects of livin and survivin gene silencing on the proliferation and apoptosis. mPEG-CS nanoparticles with sizes of approximately 60 nm were first synthesized by ionic crosslinking. Through electrostatic adsorption, mPEG-CS-livin short hairpin RNA (shRNA), mPEG-CS-survivin shRNA, and mPEG-CS-(livin shRNA + survivin shRNA) nanoparticles were then prepared to transfect PC-3M cells. The mRNA and protein expression levels of livin and survivin were measured by reverse transcription-PCR and western blotting, respectively. The inhibitory effects of down-regulated livin and survivin gene expression on the cell proliferation were evaluated by MTT assay. Cell apoptosis was assessed visually using Hoechst staining. Livin and survivin expression levels in all shRNA interference groups were effectively down-regulated at both the mRNA and protein levels. Dual silencing of livin and survivin genes markedly inhibited cell proliferation and facilitated apoptosis, with better outcomes than those of individual shRNA treatments. mPEG-CS nanoparticle-mediated dual shRNA interference of livin and survivin genes significantly reduced the expression levels in PC-3M cells, inhibited proliferation, and promoted apoptosis. As these effects were superior to single interference, this method may have synergistic effects.
authors.affiliation: Department of Urology, Ruijin Hospital Luwan Branch of Jiaotong University, Shanghai, China.
authors.forename: A Q
authors.initials: AQ
authors.lastname: Yang
authors.affiliation: Department of Urology, Ruijin Hospital Luwan Branch of Jiaotong University, Shanghai, China.
authors.forename: P J
authors.initials: PJ
authors.lastname: Wang
authors.affiliation: Department of Urology, Ruijin Hospital Luwan Branch of Jiaotong University, Shanghai, China.
authors.forename: T
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Urology, Ruijin Hospital Luwan Branch of Jiaotong University, Shanghai, China.
authors.forename: W L
authors.initials: WL
authors.lastname: Zhou
authors.affiliation: Department of Urology, UCI Medical Center, Orange, CA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Landman
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048271:Chitosan; D018698:Glutamic Acid; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D011092:Polyethylene Glycols; D011471:Prostatic Neoplasms / Q000378:metabolism*; D034622:RNA Interference; D000077022:Survivin
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C107489:BIRC5 protein, human; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D000077022:Survivin; D018698:Glutamic Acid; D011092:Polyethylene Glycols; D048271:Chitosan