title: Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: an innovative technique in food supplement quality control.
abstract: Recently, the fingerprint approach using chromatography has become one of the most effective tools for quality assessment of herbal medicines and food supplements: due to the complexity of the chromatographic fingerprint and the irreproducibility of chromatographic instruments and experimental conditions, chemometric approach is employed to deal with the chromatographic fingerprint. The study was aimed at developing new analytical methods for the multivariate phytochemical fingerprinting of bioactive compounds in eight tree-species bud-preparations, commonly used in phytotherapy. Methods was used to identify and quantify the main bioactive compounds (polyphenols, organic acids and vitamins), and obtain a specific botanical profile in order to assess the contribution of each single bioactive class to the total bud preparation phytocomplex. A chemometric approach was used to distinguish among different genotypes assuring the identity, safety and quality of the botanical raw materials. The established protocol was simple, sensitive and reliable and it could be used for the evaluation and quality control of bud-extracts and natural food supplements: the proposed method was successfully applied to the characterization of commercial bud-preparations, demonstrating to be an effective tool for the fingerprinting of this plant material. The new approach developed in this study represents a good alternative for improving the classification results of herbal materials with complex chromatograms. It should be necessary to develop a "multivariate chromatographic fingerprint", in order to differentiate the herbal preparations according to their genotype, avoiding substitutions, changes or adulterations with other species or synthetic drugs.
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Braccini 2, 10095 Grugliasco, TO Italy.
authors.forename: D
authors.initials: D
authors.lastname: Donno
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Genova, Viale Cembrano 4, 16148 Genoa, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Boggia
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Genova, Viale Cembrano 4, 16148 Genoa, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Zunin
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Braccini 2, 10095 Grugliasco, TO Italy.
authors.forename: A K
authors.initials: AK
authors.lastname: Cerutti
authors.affiliation: Geal Pharma, Strada Rivà 20, 10060 Bricherasio, TO Italy.
authors.forename: M
authors.initials: M
authors.lastname: Guido
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Braccini 2, 10095 Grugliasco, TO Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Mellano
authors.affiliation: Polytechnic of Rijeka, Trpimirova 2, 51000 Rijeka, Croatia.
authors.forename: Z
authors.initials: Z
authors.lastname: Prgomet
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, Largo Braccini 2, 10095 Grugliasco, TO Italy.
authors.forename: G L
authors.initials: GL
authors.lastname: Beccaro
keywords: HPLC; Multivariate fingerprinting; Natural food-product; Raw material control