title: Genomic copy number alterations in 33 malignant peritoneal mesothelioma analyzed by comparative genomic hybridization array.
abstract: Malignant peritoneal mesotheliomas (MPM) are rare, accounting for approximately 8% of cases of mesothelioma in France. We performed comparative genomic hybridization (CGH) on frozen MPM samples using the Agilent Human Genome CGH 180 K array. Samples were taken from a total of 33 French patients, comprising 20 men and 13 women with a mean (range) age of 58.4 (17-76) years. Asbestos exposure was reported in 8 patients (24.2%). Median (range) overall survival (OS) was 39 (0-119) months. CGH analysis demonstrated the presence of chromosomal instability in patients with MPM, with a genomic pattern that was similar to that described for pleural mesothelioma, including the loss of chromosomal regions 3p21, 9p21, and 22q12. In addition, novel genomic copy number alterations were identified, including the 15q26.2 region and the 8p11.22 region. Median OS was associated with a low peritoneal cancer index (P=.011), epithelioid subtype (P=.038), and a low number of genomic aberrations (P=.015), all of which constitute good prognostic factors for MPM. Our results provide new insights into the genetic and genomic background of MPM. Although pleural and peritoneal mesotheliomas have different risk factors, different therapeutics, and different prognosis; these data provide support to combine pleural and peritoneal mesothelioma in same clinical assays.
authors.affiliation: Department of Surgery, Hospices Civils de Lyon (HCL) Cancer Institute, Lyon 1 University, 69310 Pierre-Bénite, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Chirac
authors.affiliation: Department of Medical Oncology, HCL Cancer Institute, Lyon 1 University, 69310 Pierre-Bénite, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Maillet
authors.affiliation: Lille University, Centre Hospitalier et Universitaire (CHU) Lille, Institut Recherche Sur Le Cancer (IRCL), Structural and Functional Genomics Core Facility, 59000 Lille, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Leprêtre
authors.affiliation: Department of Pathology, HCL Cancer Institute, Lyon 1 University, 69577 Bron, France; Equipe Mixte de Recherche 3738, Lyon 1 University, Faculty of Medicine, 69310 Pierre-Bénite, France; Réseau National de Prise en Charge des Tumeurs Rares du Péritoine (RENAPE), HCL Cancer Institute and Lyon 1 University, Lyon, 69310 Pierre-Bénite, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Isaac
authors.affiliation: Department of Surgery, Hospices Civils de Lyon (HCL) Cancer Institute, Lyon 1 University, 69310 Pierre-Bénite, France; Equipe Mixte de Recherche 3738, Lyon 1 University, Faculty of Medicine, 69310 Pierre-Bénite, France; Réseau National de Prise en Charge des Tumeurs Rares du Péritoine (RENAPE), HCL Cancer Institute and Lyon 1 University, Lyon, 69310 Pierre-Bénite, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Glehen
authors.affiliation: Lille University, Centre Hospitalier et Universitaire (CHU) Lille, Institut Recherche Sur Le Cancer (IRCL), Structural and Functional Genomics Core Facility, 59000 Lille, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Figeac
authors.affiliation: Equipe Mixte de Recherche 3738, Lyon 1 University, Faculty of Medicine, 69310 Pierre-Bénite, France; Réseau National de Prise en Charge des Tumeurs Rares du Péritoine (RENAPE), HCL Cancer Institute and Lyon 1 University, Lyon, 69310 Pierre-Bénite, France; HCL, Pôle Information Médicale Evaluation Recherche (IMER), 69310 Pierre-Bénite, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Villeneuve
authors.affiliation: Department of Medical Oncology, HCL Cancer Institute, Lyon 1 University, 69310 Pierre-Bénite, France; HCL, Biostatistics Unit, Lyon, Center National de la Recherche, Scientifique Unités Mixtes de Recherche (CNRS UMR) 5558, Biometry and Evolutionary Biology Laboratory, Health and Biostatistics Team, 69100 Villeurbanne, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Péron
authors.affiliation: InVentiv Medical Communications, London WC2H 8AL, UK.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gibson
authors.affiliation: Mésothéliomes Malins Pleuraux et Tumeurs Péritonéales Rares (MESOPATH)-IM@EC, CHU Caen, 14003 Caen, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Galateau-Sallé
authors.affiliation: Department of Surgery, Hospices Civils de Lyon (HCL) Cancer Institute, Lyon 1 University, 69310 Pierre-Bénite, France; Equipe Mixte de Recherche 3738, Lyon 1 University, Faculty of Medicine, 69310 Pierre-Bénite, France; Réseau National de Prise en Charge des Tumeurs Rares du Péritoine (RENAPE), HCL Cancer Institute and Lyon 1 University, Lyon, 69310 Pierre-Bénite, France.
authors.forename: François-Noël
authors.initials: FN
authors.lastname: Gilly
authors.affiliation: Department of Pathology, HCL Cancer Institute, Lyon 1 University, 69577 Bron, France; Equipe Mixte de Recherche 3738, Lyon 1 University, Faculty of Medicine, 69310 Pierre-Bénite, France; Réseau National de Prise en Charge des Tumeurs Rares du Péritoine (RENAPE), HCL Cancer Institute and Lyon 1 University, Lyon, 69310 Pierre-Bénite, France. Electronic address: marie.brevet01@chu-lyon.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Brevet
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001194:Asbestos / Q000009:adverse effects; D014408:Biomarkers, Tumor / Q000235:genetics*; D043171:Chromosomal Instability; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations; D005260:Female; D005602:France; D005784:Gene Amplification; D018628:Gene Dosage; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D019570:Inhalation Exposure / Q000009:adverse effects; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D008297:Male; D008654:Mesothelioma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D000086002:Mesothelioma, Malignant; D008875:Middle Aged; D010534:Peritoneal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D010641:Phenotype; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D012307:Risk Factors; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D001194:Asbestos
keywords: Asbestos; Comparative genomic hybridization; Copy number alterations; Malignant peritoneal mesothelioma; VEGF-B