title: An electrochemical DNA biosensor based on Oracet Blue as a label for detection of Helicobacter pylori.
abstract: An innovative method of a DNA electrochemical biosensor based on Oracet Blue (OB) as an electroactive label and gold electrode (AuE) for detection of Helicobacter pylori, was offered. A single-stranded DNA probe with a thiol modification was covalently immobilized on the surface of the AuE by forming an Au-S bond. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization by measuring the electrochemical signals of reduction of the OB binding to double-stranded DNA (ds-DNA). Our results showed that OB-based DNA biosensor has a decent potential for detection of single-base mismatch in target DNA. Selectivity of the proposed DNA biosensor was further confirmed in the presence of non-complementary and complementary DNA strands. Under optimum conditions, the electrochemical signal had a linear relationship with the concentration of the target DNA ranging from 0.3nmolL(-1) to 240.0nmolL(-1), and the detection limit was 0.17nmolL(-1), whit a promising reproducibility and repeatability.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Hajihosseini
authors.affiliation: Department of Textile and Polymer Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran. Electronic address: nasirizadeh@iauyazd.ac.ir.
authors.forename: Navid
authors.initials: N
authors.lastname: Nasirizadeh
authors.affiliation: Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad Saeid
authors.initials: MS
authors.lastname: Hejazi
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Parichereh
authors.initials: P
authors.lastname: Yaghmaei
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D016480:Helicobacter pylori / Q000302:isolation & purification*; D013194:Staining and Labeling
chemical_list: D000880:Anthraquinones; D004277:DNA, Single-Stranded; C048853:Disperse Blue 1; D006046:Gold; D004247:DNA
keywords: DNA biosensor; Oracet Blue