title: Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma.
abstract: In the present study, we aimed to examine whether SET domain-containing methyltransferases are up-regulated in different classes of renal cell carcinoma. We immunoblotted against SET domain and quantified the expression of these modular domains. Furthermore, we examined the expression of Rad51, the key protein that confers genomic stability. There was enhanced expression of SET domain-containing histone methyltransferases in whole lysates of all classes of renal carcinoma. In metastatic high grade clear cell carcinoma, this expression was more pronounced. Though we could not demonstrate direct correlation, we showed that epigenetic modification by methylation is associated with decreased genomic translation of Rad51.
authors.affiliation: Department of Urology, The First Hospital of Jilin University, Jilin 130021, China.
authors.forename: Si
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Gynaecology, The First Hospital of Jilin University, Jilin 130021, China.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Obstetrics, The First Hospital of Jilin University, Jilin 130021, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Urology, The Third Hospital of Jilin University, Jilin 130031, China.
authors.forename: Kaichen
authors.initials: K
authors.lastname: Wang
authors.affiliation: Intensive Care Unit, The First Hospital of Jilin University, Jilin 130021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Nephrology, The First Hospital of Jilin University, Jilin 130021, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Nephrology, The First Hospital of Jilin University, Jilin 130021, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Nephrology, The First Hospital of Jilin University, Jilin 130021, China yingxu_jilin@126.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D042822:Genomic Instability / Q000235:genetics; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics; D006657:Histones / Q000235:genetics; D006801:Humans; D008297:Male; D008780:Methyltransferases / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D051135:Rad51 Recombinase / Q000235:genetics*
chemical_list: D006657:Histones; D000076983:Histone Methyltransferases; D008780:Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; D051135:Rad51 Recombinase
keywords: epigenetic; genome instability; histone; metastasis; methyltransferase; renal cell cancer