title: MicroRNA‑126 is downregulated in thyroid cancer cells, and regulates proliferation, migration and invasion by targeting CXCR4.
abstract: MicroRNA-126 (miR-126) has previously been reported to be downregulated in various types of cancer; however, at present, there are no studies of miR‑126 in human thyroid cancer. The present study aimed to determine the expression and effects of miR‑126 on thyroid cancer. The expression levels of miR‑126 were detected in human thyroid cancer tissues, matched normal adjacent tissues and human thyroid cancer cell lines using quantitative polymerase chain reaction. In addition, post‑transfection of human thyroid cancer cells with miR‑126 mimics, cell proliferation, migration and invasion assays, western blot analysis and luciferase assays were conducted. The results demonstrated that miR‑126 was downregulated in thyroid cancer tissues and cells. Furthermore, upregulation of miR‑126 inhibited cell proliferation, migration and invasion in thyroid cancer cells. The present study also provided evidence suggesting that miR‑126 may directly target C‑X‑C chemokine receptor type 4 (CXCR4) in thyroid cancer. These results indicated that miR‑126 may be investigated as a target for the treatment of thyroid cancer.
authors.affiliation: Department of Environmental Pollution and Health, State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Laboratory of Virus Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 102488, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Environmental Pollution and Health, State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, P.R. China.
authors.forename: Zhanlu
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Department of Environmental Pollution and Health, State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Occupational and Environmental Medicine, School of Public Health, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Environmental Pollution and Health, State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, P.R. China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Environmental Pollution and Health, State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, P.R. China.
authors.forename: Xianliang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D034622:RNA Interference; D019718:Receptors, CXCR4 / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000235:genetics*; D014162:Transfection
chemical_list: C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D019718:Receptors, CXCR4