title: Overexpression of Annexin II Receptor-Induced Autophagy Protects Against Apoptosis in Uveal Melanoma Cells.
abstract: Uveal melanoma is the most common primary malignant intraocular tumor in adults and still lacks effective systemic therapies. Annexin A2 receptor (AXIIR), a receptor for Annexin II, was demonstrated to play an important role in multiple cells, but its role in uveal melanoma cells remains exclusive. Herein, the authors reported that overexpression of AXIIR was able to reduce cell viability and activate apoptosis apparently in the Mum2C uveal melanoma cell line. Meanwhile, overexpression of AXIIR could induce autophagy and increase autophagy flux. After autophagy was inhibited by chloroquine, enhanced apoptosis and cytotoxicity could be detected. In summary, these data highlighted the crucial role of AXIIR in reducing Mum2C cell viability through inducing apoptosis, while autophagy played a protective role in this process. Interference of this gene may be a promising method for uveal melanoma therapy and combination with specific inhibitor of autophagy may serve as a supplementary.
authors.affiliation: 1 Department of Ophthalmology, Changhai Hospital, Second Military Medical University , Shanghai, China .
authors.forename: Yuelu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1 Department of Ophthalmology, Changhai Hospital, Second Military Medical University , Shanghai, China .
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Song
authors.affiliation: 1 Department of Ophthalmology, Changhai Hospital, Second Military Medical University , Shanghai, China .
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: 2 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University , Shanghai, China .
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: 2 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University , Shanghai, China .
authors.forename: Hailin
authors.initials: H
authors.lastname: Tang
authors.affiliation: 2 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University , Shanghai, China .
authors.forename: Zhongtian
authors.initials: Z
authors.lastname: Qi
authors.affiliation: 2 Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University , Shanghai, China .
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.affiliation: 1 Department of Ophthalmology, Changhai Hospital, Second Military Medical University , Shanghai, China .
authors.forename: Shihong
authors.initials: S
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000502:physiology; D001343:Autophagy / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018000:Receptors, Peptide / Q000096:biosynthesis* / Q000235:genetics; D014604:Uveal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000098943:Uveal Melanoma
chemical_list: D018000:Receptors, Peptide; C514873:annexin II receptor, human
keywords: Annexin II receptor; apoptosis; autophagy; human uveal melanoma