title: Biotype Characterization, Developmental Profiling, Insecticide Response and Binding Property of Bemisia tabaci Chemosensory Proteins: Role of CSP in Insect Defense.
abstract: Chemosensory proteins (CSPs) are believed to play a key role in the chemosensory process in insects. Sequencing genomic DNA and RNA encoding CSP1, CSP2 and CSP3 in the sweet potato whitefly Bemisia tabaci showed strong variation between B and Q biotypes. Analyzing CSP-RNA levels showed not only biotype, but also age and developmental stage-specific expression. Interestingly, applying neonicotinoid thiamethoxam insecticide using twenty-five different dose/time treatments in B and Q young adults showed that Bemisia CSP1, CSP2 and CSP3 were also differentially regulated over insecticide exposure. In our study one of the adult-specific gene (CSP1) was shown to be significantly up-regulated by the insecticide in Q, the most highly resistant form of B. tabaci. Correlatively, competitive binding assays using tryptophan fluorescence spectroscopy and molecular docking demonstrated that CSP1 protein preferentially bound to linoleic acid, while CSP2 and CSP3 proteins rather associated to another completely different type of chemical, i.e. α-pentyl-cinnamaldehyde (jasminaldehyde). This might indicate that some CSPs in whiteflies are crucial to facilitate the transport of fatty acids thus regulating some metabolic pathways of the insect immune response, while some others are tuned to much more volatile chemicals known not only for their pleasant odor scent, but also for their potent toxic insecticide activity.
authors.affiliation: Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Jinan, China.
authors.forename: Guoxia
authors.initials: G
authors.lastname: Liu
authors.affiliation: Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Jinan, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ma
authors.affiliation: Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Jinan, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xie
authors.affiliation: Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Jinan, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xuan
authors.affiliation: Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Jinan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Guo
authors.affiliation: Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Jinan, China.
authors.forename: Zhongxue
authors.initials: Z
authors.lastname: Fan
authors.affiliation: University of Tartu, Institute of Computer Science, 2 Liivi, Tartu, Estonia.
authors.forename: Balaji
authors.initials: B
authors.lastname: Rajashekar
authors.affiliation: University of Nantes, Protein Engineering and Functionality Unit, UMR CNRS 6286, 2 La Houssinière, Nantes, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Arnaud
authors.affiliation: University of Nantes, Protein Engineering and Functionality Unit, UMR CNRS 6286, 2 La Houssinière, Nantes, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Offmann
authors.affiliation: Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Jinan, China.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Picimbon
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002999:Clone Cells; D020224:Expressed Sequence Tags; D005453:Fluorescence; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006430:Hemiptera / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000276:immunology*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D008024:Ligands; D019787:Linoleic Acid / Q000378:metabolism; D062105:Molecular Docking Simulation; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity; D010078:Oxazines / Q000633:toxicity; D010802:Phylogeny; D000077922:Thiamethoxam; D013844:Thiazoles / Q000633:toxicity; D013997:Time Factors
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D008024:Ligands; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D013844:Thiazoles; D000077922:Thiamethoxam; D000171:Acrolein; D019787:Linoleic Acid; C012843:cinnamaldehyde