title: Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus.
abstract: OBJECTIVE
  This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C.
  
  
  METHODS
  A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR.
  
  
  RESULTS
  The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710).
  
  
  CONCLUSIONS
  Screening by ARMS - PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.
authors.affiliation: Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Fateh
authors.affiliation: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Aghasadeghi
authors.affiliation: Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Seyed D
authors.initials: SD
authors.lastname: Siadat
authors.affiliation: Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Farzam
authors.initials: F
authors.lastname: Vaziri
authors.affiliation: Department of Immunology and Microbiology, Babol University of Medical Sciences, School of Medicine, Babol, Iran.
authors.forename: Farzin
authors.initials: F
authors.lastname: Sadeghi
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
authors.forename: Roohollah
authors.initials: R
authors.lastname: Fateh
authors.affiliation: Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Keyvani
authors.affiliation: Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Alireza H
authors.initials: AH
authors.lastname: Tasbiti
authors.affiliation: Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Shamsi
authors.initials: S
authors.lastname: Yari
authors.affiliation: Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Angila
authors.initials: A
authors.lastname: Ataei-Pirkooh
authors.affiliation: Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed H
authors.initials: SH
authors.lastname: Monavari
keywords: Hepatitis C; amplification refractory mutation system (ARMS)-PCR; direct DNA sequencing; real-time polymerase chain reaction (PCR)