title: Performance of the HSV OligoGen kit for the diagnosis of herpes simplex virus type 1 and 2.
abstract: PCR methods are nowadays between the most rapid and sensitive methods for screening and diagnosing herpes simplex virus (HSV) type 1 and 2. The aim of this study was to analyze the reliability, accuracy, and usefulness of the new assay HSV OligoGen kit in comparison with the Roche LightCycler HSV ½ Qual Kit assay for the detection of HSV in clinical samples. For this analysis, a prospective study was designed for detection of HSV-1 and HSV-2 including 110 ulcer specimens, 48 urine, 48 endocervical, 43 cerebral spinal fluids, 4 urethral and 3 pharyngeal swabs that were sent from a regional STI clinic or an Intensive Clinical Unit, both in Seville, Spain. In comparison to the Roche LightCycler HSV ½ Qual Kit assay, sensitivity, specificity, positive and negative predicative values, and kappa value for HSV detection using the HSV OligoGen kit were 96.2%, 100%, 100%, 98.3%, and 0.97 for HSV-1, respectively. For HSV-2, the corresponding values were 98.3%, 100%, 100%, 99.5%, and 0.98, respectively. Statistical data obtained in this study confirms the usefulness and reliable results of this new assay.
authors.affiliation: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: manuel.parra.exts@juntadeandalucia.es.
authors.forename: Manuel
authors.initials: M
authors.lastname: Parra-Sánchez
authors.affiliation: Operon Inmuno&Molecular Diagnostics, Cuarte de Huerva, Zaragoza, Spain. Electronic address: a.marcuello@operon.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Marcuello López
authors.affiliation: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: sylvyes_@hotmail.com.
authors.forename: Silvia
authors.initials: S
authors.lastname: García-Rey
authors.affiliation: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: natilespa@gmail.com.
authors.forename: Ismail
authors.initials: I
authors.lastname: Zakariya-Yousef Breval
authors.affiliation: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: samuel.bernal.sspa@juntadeandalucia.es.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bernal Martínez
authors.affiliation: Center of Sexually Transmitted Infections of Seville, Seville, Spain. Electronic address: isabel.pueyo.sspa@juntadeandalucia.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Pueyo Rodríguez
authors.affiliation: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: estrella.martin.sspa@juntadeandalucia.es.
authors.forename: Estrella
authors.initials: E
authors.lastname: Martín-Mazuelos
authors.affiliation: Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain. Electronic address: jcpalomares@us.es.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Palomares Folía
mesh_terms: D000328:Adult; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis*; D006561:Herpes Simplex / Q000175:diagnosis*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013030:Spain; D055815:Young Adult
keywords: Diagnostic test; Genital herpes infection; HSV-1; HSV-2; Sexually transmitted infections