title: Cariogenicity features of Streptococcus mutans in presence of rubusoside.
abstract: BACKGROUND
  One promising way of reducing caries is by using sucrose substitutes in food. rubusoside is a prototype sweet substance isolated from the leaves of the plant Rubrus suavissimus S. Lee. (Rosaceae), and is rated sweeter than sucrose. The purpose of this study was to investigate the effects of rubusoside on Streptococcus mutans growth, acidogenicity, and adherence to glass in vitro.
  
  
  METHODS
  The effects of rubusoside on the growth and glass surface adhering of Streptococcus mutans were investigated by measuring the optical density of the culture at 540 nm with a spectrophotometer. Rubusoside influence on Streptococcus mutans acidogenicity was determined by measuring the pH of the culture. Sucrose, glucose, maltose, fructose and xylitol were designed to compare with rubusoside.
  
  
  RESULTS
  S. mutans growth in the rubusoside-treated group was significantly lower than that in the sucrose, glucose, maltose and fructose groups (p < 0.05) except for xylitol group (p > 0.05). Sucrose-treated S. mutans exhibited the highest adherence to glass, and rubusoside-treated S. mutans exhibited the lowest. S. mutans adherence to a glass surface and acidogenicity with sucrose were significantly reduced by rubusoside.
  
  
  CONCLUSIONS
  Rubusoside may have some potential as a non-cariogenic, non-caloric sweetener.
authors.affiliation: College of Stomatology, Zhengzhou University, Nanyang Road 169-10#, Zhengzhou, 453002, China. cjp605@zzu.edu.cn.
authors.forename: Jinpu
authors.initials: J
authors.lastname: Chu
authors.affiliation: College of Stomatology, Zhengzhou University, Nanyang Road 169-10#, Zhengzhou, 453002, China.
authors.forename: Tieting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Stomatology, Guangxi Medical University, Shuangyong Road 10#, Nanning, 530021, China.
authors.forename: Kexin
authors.initials: K
authors.lastname: He
mesh_terms: D003731:Dental Caries; D045786:Diterpenes, Kaurane; D005960:Glucosides; D013295:Streptococcus mutans / Q000472:pathogenicity*; D013395:Sucrose
chemical_list: D045786:Diterpenes, Kaurane; D005960:Glucosides; D013395:Sucrose; C056177:rubusoside
keywords: Non-cariogenic; Rubusoside; Streptococcus mutans; Sugar substitute