title: Fibronectin-1 expression is increased in aggressive thyroid cancer and favors the migration and invasion of cancer cells.
abstract: In this study we analyzed the expression levels of markers of epithelial-to-mesenchymal transition (EMT) in several papillary thyroid carcinomas (PTCs) and the relation with tumor genotypes and clinicopathological characteristics. The role of fibronectin-1 (FN1) was investigated by analyzing the effects of FN1 silencing in two human thyroid cancer cell lines. Most of EMT markers were significantly over-expressed in a group of 36 PTCs. In particular, FN1 mRNA levels were higher in tumor vs non-tumor tissue (117.3, p < 0.001) and also in aggressive and BRAF(V600E) samples. Similar results were observed (and confirmed at the protein level) when FN1 expression was analyzed in a validation group of 50 PTCs and six lymph node (LN) metastases. Silencing of FN1 in TPC-1 and BCPAP thyroid cancer cells significantly reduced proliferation, adhesion, migration, and invasion in both cell lines. Collectively, our data indicate that FN1 overexpression is an important determinant of thyroid cancer aggressiveness.
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161, Rome, Italy.
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Sponziello
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161, Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rosignolo
authors.affiliation: Department of Health Sciences, "Magna Graecia" University of Catanzaro, 88100, Catanzaro, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Celano
authors.affiliation: Department of Health Sciences, "Magna Graecia" University of Catanzaro, 88100, Catanzaro, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Maggisano
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161, Rome, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Pecce
authors.affiliation: Department of Health Sciences, "Magna Graecia" University of Catanzaro, 88100, Catanzaro, Italy.
authors.forename: Roberta Francesca
authors.initials: RF
authors.lastname: De Rose
authors.affiliation: Department of Health Sciences, "Magna Graecia" University of Catanzaro, 88100, Catanzaro, Italy.
authors.forename: Giovanni Enrico
authors.initials: GE
authors.lastname: Lombardo
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161, Rome, Italy.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Durante
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161, Rome, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Filetti
authors.affiliation: Institute of Medical Genetics, "S. Maria della Misericordia" University Hospital, 33100, Udine, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Damante
authors.affiliation: Department of Health Sciences, "Magna Graecia" University of Catanzaro, 88100, Catanzaro, Italy. Electronic address: d.russo@unicz.it.
authors.forename: Diego
authors.initials: D
authors.lastname: Russo
authors.affiliation: Department of Health Sciences, "Magna Graecia" University of Catanzaro, 88100, Catanzaro, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Bulotta
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D011446:Prospective Studies; D012333:RNA, Messenger / Q000235:genetics; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C067625:FN1 protein, human; D005353:Fibronectins; D012333:RNA, Messenger
keywords: BRAF; EMT; Fibronectin; Thyroid cancer