title: Pleiotropic effect of anionic phospholipids absence on mitochondrial morphology and cell wall integrity in strictly aerobic Kluyveromyces lactis yeasts.
abstract: Cardiolipin and phosphatidylglycerol are anionic phospholipids localized to the inner mitochondrial membrane. In this study, it is demonstrated by fluorescence and transmission electron microscopy that atp2.1pgs1Δ mutant mitochondria lacking anionic phospholipids contain fragmented and swollen mitochondria with a completely disorganized inner membrane. In the second part of this study, it was shown that the temperature sensitivity of the atp2.1pgs1Δ mutant was not suppressed by the osmotic stabilizer glucitol but by glucosamine, a precursor of chitin synthesis. The atp2.1pgs1Δ mutant was hypersensitive to Calcofluor White and caffeine, resistant to Zymolyase, but its sensitivity to caspofungin was the same as the strains with the standard PGS1 gene. The distribution of chitin in the mutant cell wall was impaired. The glucan level in the cell wall of the atp2.1pgs1Δ mutant was reduced by 4-8 %, but the level of chitin was almost double that in the wild-type strain. The cell wall of the atp2.1pgs1Δ mutant was about 20 % thinner than the wild type, but its morphology was not significantly altered.
authors.affiliation: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Ilkovicova 6, Mlynska dolina B-2, Bratislava, 842 15, Slovak Republic.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Bardelcikova
authors.affiliation: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Ilkovicova 6, Mlynska dolina B-2, Bratislava, 842 15, Slovak Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Drozdikova
authors.affiliation: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Ilkovicova 6, Mlynska dolina B-2, Bratislava, 842 15, Slovak Republic. obernauerova@fns.uniba.sk.
authors.forename: Margita
authors.initials: M
authors.lastname: Obernauerova
mesh_terms: D000332:Aerobiosis; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure*; D017353:Gene Deletion; D005936:Glucans / Q000032:analysis; D007716:Kluyveromyces / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure*; D010743:Phospholipids / Q000172:deficiency*; D013696:Temperature
chemical_list: D005936:Glucans; D010743:Phospholipids