title: The effects of elevated carbon dioxide and temperature levels on tilapia (Oreochromis mossambicus): Respiratory enzymes, blood pH and hematological parameters.
abstract: Oreochromis mossambicus were exposed to two different temperature and carbon dioxide partial pressure levels for about two weeks, as the ambient (Control; 25°C, 3.3mg/L CO2), high CO2 (25°C, 14mg/L CO2), high temperature (30°C, 3mg/L CO2) and combined (30°C, 14.1mg/L CO2) groups. No mortality was observed during the experiments. As a result of the study, elevated CO2 concentrations cause negative effects on the hematological parameters. At the end of the study, while the blood Carbonic Anhydrase (CA) activity, in the high CO2 group (25°C, 14mg/L CO2), statistically increased at the 7th day compared to the control group, it decreased at the 14th day (p<0.05). In addition, the blood CA activity, in the combined (30°C, 14.1mg/L CO2) group, showed a decrease at the 14th day compared to the control group (p<0.05). At the end of study, unlike the blood CA activity, gill, liver and kidney CA activity showed an increase in the tissues compared to the control groups (p<0.05). Furthermore, the Na(+), K(+)-ATPase activities were stimulated significantly in the gills in both high CO2 and temperature groups at day 7, but it showed a significant amount of inhibition at the 14th day compared to the control groups. Overall, increasing carbon dioxide concentration in different temperatures has negative effects on the hematological parameters and respiratory enzyme of the tilapia fish. In addition, it is observed that the fish survive at negative conditions with adaptation mechanisms.
authors.affiliation: Department of Basic Sciences, Marine Sciences and Technology Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Turkey. Electronic address: hasankaya@comu.edu.tr.
authors.forename: Hasan
authors.initials: H
authors.lastname: Kaya
authors.affiliation: Department of Basic Sciences, Marine Sciences and Technology Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Olcay
authors.initials: O
authors.lastname: Hisar
authors.affiliation: Department of Aquaculture, Marine Sciences and Technology Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Sevdan
authors.initials: S
authors.lastname: Yılmaz
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Gürkan
authors.affiliation: Department of Food Engineering, Engineering Faculty, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
authors.forename: Şükriye Aras
authors.initials: ŞA
authors.lastname: Hisar
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000097:blood / Q000378:metabolism; D029941:Fish Proteins / Q000097:blood / Q000378:metabolism; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D006403:Hematologic Tests; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013696:Temperature; D017210:Tilapia / Q000097:blood / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Blood pH; Carbonic anhydrase; Elevated carbon dioxide and temperature; Hematology; K(+)-ATPase; Na(+); Oreochromis mossambicus