title: In-capillary derivatization with o-phthalaldehyde in the presence of 3-mercaptopropionic acid for the simultaneous determination of monosodium glutamate, benzoic acid, and sorbic acid in food samples via capillary electrophoresis with ultraviolet detection.
abstract: For the rapid simultaneous determination of monosodium glutamate (MSG), benzoic acid (BA), and sorbic acid (SA) in canned food and other processed food samples, we developed a method that combines in-capillary derivatization with separation by capillary electrophoresis. This method employs the rapid derivatization of MSG with o-phthalaldehyde (OPA) in the presence of 3-mercaptopropionic acid (3-MPA) and enables the detection of the resulting OPA-MSG derivative and of non-derivatized BA and SA at 230nm. The composition of the background electrolyte and the parameters of derivatization and separation are as follows: 25mM borax containing 5mM OPA and 6mM 3-MPA, separation voltage 25mV, injection at 30mbar for 20s, and column temperature 25°C. Because of the high reaction rate and suitably adapted effective electrophoretic mobilities, band broadening due to the derivatization reaction at the start of the separation process is kept to a minimum. The optimized method is validated with respect to LOD, LOQ, linearity, recovery, and precision. This method can be applied to real samples such as soy, fish, oyster and sweet and sour chili sauces after application of appropriate clean-up steps. Mechanisms of zone broadening and zone focusing are discussed showing the validity of the employed theoretical approach regarding the dependence of the peak shape for OPA-MSG on the concentration of MSG in the sample.
authors.affiliation: Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia; Centre for Bio-Diversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia. Electronic address: hninpa@utar.edu.my.
authors.forename: Hnin-Pwint
authors.initials: HP
authors.lastname: Aung
authors.affiliation: Department of Chemistry, University of Marburg, Hans-Meerwein-Strasse, D-35032 Marburg, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Pyell
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry*; D000818:Animals; D019817:Benzoic Acid / Q000032:analysis*; D002212:Capsicum; D003212:Condiments / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods; D005399:Fishes; D005504:Food Analysis / Q000379:methods*; D007202:Indicators and Reagents; D010125:Ostreidae / Q000737:chemistry; D012970:Sodium Glutamate / Q000032:analysis*; D013011:Sorbic Acid / Q000032:analysis*; D045730:Soy Foods / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D009764:o-Phthalaldehyde / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D009764:o-Phthalaldehyde; D019817:Benzoic Acid; D015097:3-Mercaptopropionic Acid; D012970:Sodium Glutamate; D013011:Sorbic Acid
keywords: Benzoic acid; Capillary electrophoresis; In-capillary derivatization; Monosodium glutamate; Sorbic acid; o-Phthalaldehyde