title: Prevalence of Bovine Mastitis Pathogens in Bulk Tank Milk in China.
abstract: The objectives of this study were to estimate the herd prevalence of major mastitis pathogens in bulk tank milk (BTM) in China dairy herds, to determine the relationship between the presence of mastitis pathogens and bulk tank milk somatic cell counts (BTSCC), and to investigate the impact of different dairy cattle farming modes and region on bacterial species. BTM samples collected from 894 dairy herds in China were examined for the presence of mastitis pathogens. The Flinders Technology Associates (FTA) cards were used for BTM sample collection, storage, and transportation and bacterial DNA amplification by real-time PCR. Among contagious pathogens, Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus dysgalactiae were detected in 50.1, 92.2, and 72.3% of the 894 BTM samples, respectively. Among environmental pathogens, E. coli, Streptococcus uberis, Enterococcus spp., Klebsiella spp., Serratia marcescens, Corynebacterium bovis, and Arcanobacterium pyogenes were detected in 28.6, 8.9, 35.7, 20.0, 1.3, 17.0, and 67.2% of the BTM samples, respectively. Staphylococcal β-lactamase gene was detected in 61.7% of the BTM samples. The presence of Staphylococcus aureus and Arcanobacterium pyogenes were significantly associated with high BTSCC, respectively. Significant differences were found in presence of Staphylococcus aureus, Streptococcus agalactiae, and Streptococcus dysgalactiae in BTM sampled from the small household farms, dairy-farming communities, and large-scaled dairy farms. There were significant differences in the presence of Streptococcus agalactiae, Streptococcus dysgalactiae, Arcanobacterium pyogenes, staphylococcal β-lactamase gene, Staphylococcus spp., Klebsiella spp., Enterococcus spp., and Streptococcus uberis in BTM among Inner Mongolia, Heilongjiang, and Hebei province. In conclusion, contagious mammary pathogens are predominated among pathogens in BTM samples in China.
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safty Control, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Yanliang
authors.identifier: 0000-0001-8183-8208
authors.initials: Y
authors.lastname: Bi
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safty Control, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Ya Jing
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Laboratorios Hipra S.A., Amer Giron, Spain.
authors.forename: Yun
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Laboratorios Hipra S.A., Amer Giron, Spain.
authors.forename: Roger
authors.initials: R
authors.lastname: Guix Vallverdú
authors.affiliation: Laboratorios Hipra S.A., Amer Giron, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Maldonado García
authors.affiliation: Laboratorios Hipra S.A., Amer Giron, Spain.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safty Control, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safty Control, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Cao
mesh_terms: D000818:Animals; D002417:Cattle; D002681:China / Q000453:epidemiology; D003612:Dairying / Q000379:methods; D005260:Female; D008414:Mastitis, Bovine / Q000453:epidemiology* / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction