title: Correlation Study Between IL-28B Gene Polymorphism (rs8099917SNP) and Sustained Virological Response in Iranian Patients with Chronic Hepatitis C.
abstract: BACKGROUND
  The current standard treatment for hepatitis C is a combination of pegylated interferon alpha and ribavirin (peg-IFNα/RBV). Recent studies have shown that single nucleotide polymorphisms (SNPs) near the interleukin 28B (IL28B) gene coding for IFN-λ3 were associated with the antiviral treatment response. Therefore, in this study, we determined the distribution of the rs8099917 (T/G) polymorphism with sustained virological response (SVR) to chronic hepatitis C virus infection among Iranian patients.
  
  
  METHODS
  This cross-sectional study was performed on 150 blood samples based on 93 patients with chronic HCV genotypes 1 and 3 including 71 SVR positive, 22 negative, and 57 healthy individual controls. DNA was extracted from the samples and the frequency of the polymorphism was analyzed the using PCR-RFLP method. Finally, the products were detected on 3.5% agarose gel electrophoresis.
  
  
  RESULTS
  The analysis of the data for G/T polymorphism showed that the GG genotype was identified in 6 patients of 71 who achieved SVR, while the GT heterozygous was found in 33 patients and SVR was achieved in 19. Finally, the TT was detected in 53 patients and 7 patients were resistant to treatment.
  
  
  CONCLUSIONS
  The results showed significant effects of G allele carriers on susceptibility to HCV infection com-pared to the other allele (T) in our studied population (p = 0.013, OR = 2.23, 95% CI = 1.18-4.21), but we did not find a significant correlation for SVR to therapy in patients with genotype TT (p = 0.055, OR = 0.48, 95% CI = 0.23-1.01). However, further studies with more samples are necessary.
authors.forename: Mona Sadat
authors.initials: MS
authors.lastname: Larijani
authors.forename: Seyed Mehdi
authors.initials: SM
authors.lastname: Sadat
authors.forename: Mehri
authors.initials: M
authors.lastname: Nikbin
authors.forename: Seyedeh Solmaz
authors.initials: SS
authors.lastname: Talebi
authors.forename: Foozieh
authors.initials: F
authors.lastname: Javadi
authors.forename: Nasir
authors.initials: N
authors.lastname: Mohajel
authors.forename: Azam
authors.initials: A
authors.lastname: Bolhassani
authors.forename: Mahtab
authors.initials: M
authors.lastname: Daneshvar
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Aghasadeghi
authors.forename: Mohammad Hassan
authors.initials: MH
authors.lastname: Pouriayevali
authors.forename: Maryam
authors.initials: M
authors.lastname: Mapar
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000821:virology; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda