title: Experimental study on inhibition of the growth of human adenoid cystic cancer cells by RNA interference targeting against survivingene.
abstract: OBJECTIVE
  To observe the influence of RNA interference targeting against survivin gene on the biological behaviors of human adenoid cystic cancer (ACC) cells and propose the action mechanism.
  
  
  METHODS
  Specific siRNA (small interfering RNA) was constructed and transfected into ACC-2 cells using liposomes. The expressions of survivin and Caspase-3 in the transfected ACC-2 cells were detected by Western Blot and RT-PCR. Cell apoptosis was detected by transmission electron microscopy, TUNEL method and flow cytometry; ultrastructural changes and cell cycles were observed.
  
  
  RESULTS
  Recombinant siRNA interference plasmid specifically targeting against survivin gene was constructed successfully. Survivin protein expression in the transfected ACC-2 cells was downregulated significantly, while Caspase-3 protein and mRNA expressions were upregulated and cell proliferation was inhibited considerably.
  
  
  CONCLUSIONS
  Recombinant siRNA interference plasmid inhibited survivin mRNA and protein expressions at high efficiency, thereby inhibiting the proliferation of ACC cells.
authors.affiliation: Department of Stomatology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Stomatology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Congji
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Stomatology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Stomatology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Xia
keywords: Human adenoid cystic cancer cells; cell apoptosis; cell cycle; gene expression; proliferation; siRNA; survivin