title: NMR analysis on the sialic acid-binding mechanism of an R-type lectin mutant by natural evolution-mimicry.
abstract: A sialic acid-binding lectin (SRC) was created from the C-terminal domain of an R-type N-acetyl lactosamine-binding lectin (EW29Ch) by natural evolution-mimicry. Here, we clarified its sialic acid-binding mechanism using NMR spectroscopy. The NMR analysis showed differences between conformations of the 6'-sialyllactose-bound SRC in the solution state and that in the crystal state, and differences between the internal motion of the loop region in subdomain γ in SRC and that of the corresponding region in EW29Ch. The NMR analysis thus provided useful information to explain the manner of binding to 6'-sialyllactose in solution, which the previous X-ray crystal structure analysis lacked.
authors.affiliation: Food Research Institute, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Hemmi
authors.affiliation: Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.|Glycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.affiliation: Glycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Unno
authors.affiliation: Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
mesh_terms: D037161:Galectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D000072417:Protein Domains
chemical_list: C403777:6'-sialyllactose; C542531:EW29 lectin, Lumbricus terrestris; D037161:Galectins; D019158:N-Acetylneuraminic Acid; D007785:Lactose
keywords: NMR spectroscopy; R-type sialic acid-binding lectin; natural evolution-mimicry