title: Isolation of Human Amnion Epithelial Cells According to Current Good Manufacturing Procedures.
abstract: Different cell types can be isolated from human placental tissues, and some have been reported to retain phenotypic plasticity and characteristics that make them a promising source of cells for regenerative medicine. Among these are human amnion epithelial cells (hAECs). Adoption of current good manufacturing practices (cGMP) and enhanced quality control is essential when isolating hAECs in order to deliver a safe and effective cellular product for clinical purposes. This unit describes a detailed protocol for selective isolation of hAECs from human term placenta with little to no contamination by other cell types. A method for characterizing the heterogeneity of the hAEC suspension is also provided. The resulting cell product will be useful for clinical as well as basic research applications. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Roberto
authors.initials: R
authors.lastname: Gramignoli
authors.affiliation: Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Raghuraman C
authors.initials: RC
authors.lastname: Srinivasan
authors.affiliation: Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Kannisto
authors.affiliation: Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Strom
mesh_terms: D000650:Amnion / Q000166:cytology*; D002469:Cell Separation / Q000379:methods* / Q000592:standards*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010920:Placenta / Q000166:cytology; D011247:Pregnancy
keywords: amnion; amnion epithelial cells; cGMP; placenta