title: Anti-Müllerian hormone reduces growth rate without altering follicular survival in isolated caprine preantral follicles cultured in vitro.
abstract: The aim of the present study was to evaluate the effect of anti-Müllerian hormone (AMH), with and without FSH, on the in vitro development of isolated caprine preantral follicles, as well as follicular steroid production and mRNA levels of AMH, hormone receptors (AMH and FSH), CYP19A1 (cytochrome P450, family 19, subfamily A, polypeptide 1), CYP17 (cytochrome P450, family 17, subfamily A, polypeptide 1), HSD3B (3-beta-hydroxysteroid dehydrogenase) and Myc (myelocytomatosis oncogene). Isolated secondary follicles were cultured in minimum essential medium alpha (α-MEM+) alone or supplemented with 50ng mL-1AMH and/or 100ng mL-1FSH added sequentially on different days of culture. Follicles were cultured for a total of 18 days, with different media during the first (Days 0-9) and second (Days 10-18) halves of the culture period, resulting in six treatment groups, as follows: α-MEM+/α-MEM+, FSH/FSH, AMH/AMH, AMH+FSH/AMH+FSH, AMH/FSH, and FSH/AMH. Follicle development was evaluated on the basis of follicular growth, oocyte maturation and steroid secretion. There was a decrease in follicular growth rate in the AMH, AMH+FSH and AMH/FSH treatment groups compared with α-MEM+ and FSH treatment groups (P<0.05). However, the different culture conditions had no effect on rates of meiotic resumption and steroid secretion (P>0.05). Moreover, follicles cultured in the presence of FSH had lower levels of AMH receptor type II (AMHRII) mRNA compared with non-cultured control (freshly isolated follicles), and the AMH and AMH/FSH treatment groups. In conclusion, AMH reduces the follicular growth rate of isolated goat preantral follicles in vitro without affecting follicular survival.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: R M P
authors.initials: RMP
authors.lastname: Rocha
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Lima
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: I R
authors.initials: IR
authors.lastname: Brito
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: G M
authors.initials: GM
authors.lastname: Silva
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: H H V
authors.initials: HHV
authors.lastname: Correia
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: N A
authors.initials: NA
authors.lastname: Ribeiro de Sá
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: A C A
authors.initials: ACA
authors.lastname: Ferreira
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: A D
authors.initials: AD
authors.lastname: Sales
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: C H
authors.initials: CH
authors.lastname: Lobo
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B-1090 Brussels, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Smitz
authors.affiliation: Department of Animal Sciences, University of Illinois, 1207 West Gregory Drive, Urbana, IL 61801, USA.
authors.forename: M B
authors.initials: MB
authors.lastname: Wheeler
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles -LAMOFOPA, Faculty of Veterinary Medicine, State University of Ceará, Av. Paranjana 1700, Campus do Itaperi, Fortaleza, 60740-903 CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000003:Abattoirs; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D001938:Brazil; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003433:Crosses, Genetic; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006041:Goats; D006801:Humans; D009866:Oogenesis / Q000187:drug effects; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D011962:Receptors, FSH / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D018000:Receptors, Peptide / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D013739:Testosterone / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D011962:Receptors, FSH; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; D011994:Recombinant Proteins; C084621:anti-Mullerian hormone receptor; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone