title: Association of miR-146aC>G, miR-149C>T, miR-196a2T>C, and miR-499A>G polymorphisms with risk of recurrent implantation failure in Korean women.
abstract: OBJECTIVE
  The aim of this study was to investigate the association of microRNA polymorphisms (miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G) in Korean patients with recurrent implantation failure (RIF).
  
  
  METHODS
  We conducted a case-control study of 354 Korean women: 120 patients with RIF and 234 healthy controls with at least one live birth and no history of pregnancy loss.
  
  
  RESULTS
  The combined miR-146aCG+GG/miR-196a2CC genotype was more frequent in patients than in controls (P<0.05), and apparently conferred increased susceptibility. Conversely, genotype-based multifactor dimensionality reduction analysis, revealed that the G-T-T-A (miR-146a/-149/-196a2/-499) and G-T-T inferred genotypes (miR-146a/-149/-196a2) were significantly less frequent in patients, which suggested potential protective effects. The expression of miR-146a for the GG homozygote was significantly lower (P<0.05) than expression of the CC homozygote from both the pre, mature and sequences of miR-146a-3p (P<0.05 each). The expression of miR-196a2 for the CC homozygote was also lower than the TT homozygote from the mature sequence of miR-196a2-3p (P<0.05).
  
  
  CONCLUSIONS
  These results suggest that the polymorphisms in miR-146a and miR-196a2 could alter their target mRNA expression. Our findings suggest that expression levels of miR-146aC>G, miR-196a2T>C and putative gene-gene interaction between miR-146a, miR-196a2, miR-149 may be involved in RIF development in Korean women.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, 463-712, South Korea.
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Biological Science, College of Natural Science, Kongju National University, Gongju, South Korea.
authors.forename: Ki Wha
authors.initials: KW
authors.lastname: Chung
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea.
authors.forename: Jung Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea.
authors.forename: Hyogeun
authors.initials: H
authors.lastname: Jang
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea.
authors.forename: Jung Ki
authors.initials: JK
authors.lastname: Yoo
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea.
authors.forename: Youngsok
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea.
authors.forename: Jung Jae
authors.initials: JJ
authors.lastname: Ko
authors.affiliation: Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.
authors.forename: Ji Hyang
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Endocrinology and Diabetes, School of Medicine, Fukuoka University, 814-0180, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishi
authors.affiliation: Department of Endocrinology and Diabetes, School of Medicine, Fukuoka University, 814-0180, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yanase
authors.affiliation: Fertility Center of CHA Gangnam Medical Center, CHA University, Gangnam, 135-081, South Korea. Electronic address: wooslee@cha.ac.kr.
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-400, South Korea; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, 463-712, South Korea. Electronic address: nkkim@cha.ac.kr.
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Kim
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D056910:Republic of Korea; D055815:Young Adult
chemical_list: D035683:MicroRNAs
keywords: MicroRNA; Recurrent implantation failure; Single nucleotide polymorphism