title: An Efficient Protection-Free One-Pot Chemical Synthesis of Modified Nucleoside-5'-Triphosphates.
abstract: A simple, reliable, and an efficient "one-pot, three step" chemical method for the synthesis of modified nucleoside triphosphates such as 5-methylcytidine-5'-triphosphate (5-MeCTP), pseudouridine-5'-triphosphate (pseudoUTP) and N(1)-methylpseudouridine-5'-triphosphate (N(1)-methylpseudoUTP) starting from the corresponding nucleoside is described. The overall reaction involves the monophosphorylation of nucleoside, followed by the reaction with pyrophosphate and subsequent hydrolysis of the cyclic intermediate to furnish the corresponding NTP in moderate yields with high purity (>99.5%).
authors.affiliation: a Life Sciences Solutions Group, Thermo Fisher Scientific , Austin , Texas , USA.
authors.forename: Muthian
authors.initials: M
authors.lastname: Shanmugasundaram
authors.affiliation: a Life Sciences Solutions Group, Thermo Fisher Scientific , Austin , Texas , USA.
authors.forename: Annamalai
authors.initials: A
authors.lastname: Senthilvelan
authors.affiliation: a Life Sciences Solutions Group, Thermo Fisher Scientific , Austin , Texas , USA.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: a Life Sciences Solutions Group, Thermo Fisher Scientific , Austin , Texas , USA.
authors.forename: Anilkumar R
authors.initials: AR
authors.lastname: Kore
mesh_terms: D017553:Phosphorus Compounds / Q000737:chemistry; D010766:Phosphorylation; D012265:Ribonucleotides / Q000138:chemical synthesis*
chemical_list: D017553:Phosphorus Compounds; D012265:Ribonucleotides; C013196:phosphoryl chloride
keywords: Ribonucleotides; gene therapy; mRNA; one-pot synthesis