title: Characterization of the genetic environment of the blaKPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital.
abstract: Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of blaKPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by blaKPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the blaKPC-2 gene. ST423, ST65, ST977, and ST11 were all detected in KPC-2-producing K. pneumoniae. Two types of blaKPC-2-bearing genetic structure were found: Tn1721-blaKPC-2-Tn3 and Tn1721-blaKPC-2-ΔTn3-IS26; and were carried in IncX and IncFII plasmids, respectively. In conclusion, the genetic environment of the blaKPC-2 gene was diverse and Tn1721-blaKPC-2-ΔTn3-IS26 was dominant in clinical K. pneumoniae isolates in Huashan Hospital. This study sheds some light on the genetic environment and should foster further studies about the mechanism of the blaKPC-2 dissemination.
authors.affiliation: Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China.
authors.forename: Pinghua
authors.initials: P
authors.lastname: Shen
authors.affiliation: Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China; Hospital of Fudan University, Department of Laboratory Medicine, Obstetrics and Gynecology, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China; Fudan University, Shanghai Medical College, Jinshan Hospital, Department of Laboratory Medicine, Shanghai, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Fudan University, Shanghai Medical College, Huashan Hospital, Department of Laboratory Medicine, Shanghai, China. Electronic address: jiangxi2154@aliyun.com.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Jiang
mesh_terms: D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C547585:beta-lactamase KPC-2, Klebsiella pneumoniae
keywords: Genetic environment; Klebsiella pneumoniae; bla(KPC)