title: Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic Response.
abstract: NK cell heterogeneity has primarily been studied either on the population level, measuring average responses, or on the single cell level by flow cytometry, providing static snapshots. These approaches have certain drawbacks, not enabling dynamic observations of single cells over extended periods of time. One of the primary limitations of single cell imaging has been throughput; it has been challenging to collect data for many cells due to their dynamic nature and migrating out of the field of view. Spatially confining cells combined with automated fluorescence microscopy enables the simultaneous monitoring of many NK cells in parallel for extended periods of time (>12 h). Such an approach allows us to dissect how the sum of individual NK cell responses translates to the global average response typically observed.
authors.affiliation: Department of Applied Physics, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Bruno
authors.initials: B
authors.lastname: Vanherberghen
authors.affiliation: Department of Applied Physics, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Frisk
authors.affiliation: Microbiology, Tumor and Cell Biology, Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Elin
authors.initials: E
authors.lastname: Forslund
authors.affiliation: Department of Applied Physics, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Per E
authors.initials: PE
authors.lastname: Olofsson
authors.affiliation: Department of Applied Physics, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Karolin
authors.initials: K
authors.lastname: Guldevall
authors.affiliation: Department of Applied Physics, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden. onfelt@kth.se.|Microbiology, Tumor and Cell Biology, Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden. onfelt@kth.se.
authors.forename: Björn
authors.initials: B
authors.lastname: Önfelt
mesh_terms: D002465:Cell Movement; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology*; D046208:Microchip Analytical Procedures; D008856:Microscopy, Fluorescence; D059010:Single-Cell Analysis / Q000379:methods*; D059008:Time-Lapse Imaging / Q000379:methods*
keywords: Cell migration; Cell motility; Cytotoxicity; Fluorescence; Microchip; Natural killer cells; Single cell imaging