title: Dehydroepiandrosterone protects male and female hippocampal neurons and neuroblastoma cells from glucose deprivation.
abstract: Dehydroepiandrosterone (DHEA) modulates neurogenesis, neuronal function, neuronal survival and metabolism, enhancing mitochondrial oxidative capacity. Glucose deprivation and hypometabolism have been implicated in the mechanisms that mediate neuronal damage in neurological disorders, and some studies have shown that these mechanisms are sexually dimorphic. It was also demonstrated that DHEA is able to attenuate the hypometabolism that is related to some neurodegenerative diseases, eliciting neuroprotective effects in different experimental models of neurodegeneration. The aim of this study was to evaluate the effect of DHEA on the viability of male and female hippocampal neurons and SH-SY5Y neuroblastoma cells exposed to glucose deprivation. It was observed that after 12h of pre-treatment, DHEA was able to protect SH-SY5Y cells from glucose deprivation for 6h (DHEA 10(-12), 10(-8) and 10(-6)M) and 8h (DHEA 10(-8)M). In contrast, DHEA was not neuroprotective against glucose deprivation for 12 or 24h. DHEA (10(-8)M) also protected SH-SY5Y cells when added together or even 1h after the beginning of glucose deprivation (6h). Furthermore, DHEA (10(-8)M) also protected primary neurons from both sexes against glucose deprivation. In summary, our findings indicate that DHEA is neuroprotective against glucose deprivation in human neuroblastoma cells and in male and female mouse hippocampal neurons. These results suggest that DHEA could be a promising candidate to be used in clinical studies aiming to reduce neuronal damage in people from both sexes.
authors.affiliation: Laboratório de Interação Neuro-Humoral, Department of Physiology, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil; Instituto Cajal, CSIC, Avenida Doctor Arce, 37, 28002 Madrid, Spain. Electronic address: clauvmarques@yahoo.com.br.
authors.forename: Claudia
authors.initials: C
authors.lastname: Vieira-Marques
authors.affiliation: Laboratório de Interação Neuro-Humoral, Department of Physiology, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil; Instituto Cajal, CSIC, Avenida Doctor Arce, 37, 28002 Madrid, Spain.
authors.forename: Bruno Dutra
authors.initials: BD
authors.lastname: Arbo
authors.affiliation: Instituto Cajal, CSIC, Avenida Doctor Arce, 37, 28002 Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Ruiz-Palmero
authors.affiliation: Instituto Cajal, CSIC, Avenida Doctor Arce, 37, 28002 Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Ortiz-Rodriguez
authors.affiliation: Instituto Cajal, CSIC, Avenida Doctor Arce, 37, 28002 Madrid, Spain.
authors.forename: Samar
authors.initials: S
authors.lastname: Ghorbanpoor
authors.affiliation: Laboratório de Interação Neuro-Humoral, Department of Physiology, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Kucharski
authors.affiliation: Instituto Cajal, CSIC, Avenida Doctor Arce, 37, 28002 Madrid, Spain.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Arevalo
authors.affiliation: Instituto Cajal, CSIC, Avenida Doctor Arce, 37, 28002 Madrid, Spain.
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Garcia-Segura
authors.affiliation: Laboratório de Interação Neuro-Humoral, Department of Physiology, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
authors.forename: Maria Flávia M
authors.initials: MF
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D061251:Primary Cell Culture
chemical_list: D018696:Neuroprotective Agents; D003687:Dehydroepiandrosterone; D005947:Glucose
keywords: Apoptosis; DHEA; Ischemia; Neurodegenerative diseases; Neuroprotection