title: MicroRNA let-7b induces lens epithelial cell apoptosis by targeting leucine-rich repeat containing G protein-coupled receptor 4 (Lgr4) in age-related cataract.
abstract: Owing to a rapidly aging population, vision impairment caused by age-related cataract has become very common. Age-related cataract has also become one of the principal causes of blindness, and apoptosis of lens epithelial cells contributes to non-congenital cataract development. Previous studies have reported that microRNA let-7b (let-7b) is upregulated in cataractous lens epithelial cells, and the expression level of let-7b is positively associated with N, C and P cataract scores. However, the role of let-7b in the development of age-related cataract remains unclear. Here, we observed that the expression level of let-7b in the anterior lens capsules of age-related cataract was significantly higher than that in the normal anterior lens capsules. We performed ultraviolet (UV) irradiation to induce lens epithelial cell apoptosis. The results showed that the expression level of let-7b in lens epithelial cells which were treated by UV irradiation was significantly higher than that in the control, and let-7b promoted UV irradiation-induced apoptosis. Furthermore, we showed that leucine-rich repeat containing G protein-coupled receptor 4 (Lgr4) was a direct target of let-7b, and let-7b modulated lens epithelial cell apoptosis by directly targeting Lgr4. These findings will offer new insights into our understanding of the molecular mechanisms underlying the pathogenesis of cataract.
authors.affiliation: Eye Hospital of the Second Bethume Hospital, Jilin University, Changchun 130051, PR China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Eye Hospital of the Second Bethume Hospital, Jilin University, Changchun 130051, PR China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Eye Hospital of the Second Bethume Hospital, Jilin University, Changchun 130051, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Eye Hospital of the Second Bethume Hospital, Jilin University, Changchun 130051, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Eye Hospital of the Second Bethume Hospital, Jilin University, Changchun 130051, PR China. Electronic address: ms01zhao@163.com.
authors.forename: Meisheng
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects; D002386:Cataract / Q000378:metabolism* / Q000503:physiopathology; D002460:Cell Line; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism* / Q000528:radiation effects; D007930:Leucine / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects; D015854:Up-Regulation
chemical_list: C411562:LGR4 protein, human; D035683:MicroRNAs; D043562:Receptors, G-Protein-Coupled; C487931:mirnlet7 microRNA, human; D007930:Leucine
keywords: Age-related cataract; Lens epithelial cells; Lgr4; MicroRNA let-7b; Ultraviolet irradiation