title: DNA barcoding of three species (Canis aureus, Canis lupus and Vulpes vulpes) of Canidae.
abstract: Sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene have been used for DNA barcoding and determining the genetic diversity of mammal species. In the current study, our intention was to test the validity of COI barcodes for detecting genetic divergence and to reveal whether or not there is a genetic variation at this marker within canids. Three species (Canis aureus, Canis lupus and Vulpes vulpes) from the family Canidae were selected for DNA barcoding using samples collected from Iran and Turkey. All three species had unique barcoding sequences and none of the sequences were shared among these species. The mean sequence divergences within and among the species were 0.61% and 12.32%, respectively, which fell into the mean divergence ranges found in some mammal groups. The genetic diversity of these three canid species was relatively higher than that found in previously reported studies.
authors.affiliation: a Graduate School of Natural and Applied Sciences , Erciyes University , Kayseri , Turkey.
authors.forename: Eren
authors.initials: E
authors.lastname: Aksöyek
authors.affiliation: b Department of Agricultural Biotechnology, Faculty of Agriculture , Erciyes University , Kayseri , Turkey.|c Genome and Stem Cell Center, GENKOK, Erciyes University , Kayseri , Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: İbiş
authors.affiliation: c Genome and Stem Cell Center, GENKOK, Erciyes University , Kayseri , Turkey.|d Department of Biology, Faculty of Sciences , Erciyes University , Kayseri , Turkey.
authors.forename: Servet
authors.initials: S
authors.lastname: Özcan
authors.affiliation: e Department of Biology, Faculty of Science , University of Zanjan , Zanjan , Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Moradi
authors.affiliation: d Department of Biology, Faculty of Sciences , Erciyes University , Kayseri , Turkey.
authors.forename: Coşkun
authors.initials: C
authors.lastname: Tez
mesh_terms: D000818:Animals; D046028:Canidae / Q000145:classification* / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D007492:Iran; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity; D014421:Turkey
chemical_list: D003576:Electron Transport Complex IV
keywords: COI; Canidae; DNA barcoding; Iran; Turkey