title: p53- and Caspase-3-Independent Mechanism of Acetaminophen Effect on Human Neural Cells.
abstract: Acetaminophen in a concentration of 5 mM increased the expression of JNK, HIF1A (hypoxiainduced factor), and CASP3, which indicated development of oxidative stress and apoptotic cell death. Acetaminophen in a concentration of 10 mM did not induce expression of HIF1A and CASP3, but reduced expression of chaperone HSP90, which attested to activation of a caspase-3-independent mechanism of cell death. The methods of preventing acetaminophen intoxication are discussed.
authors.affiliation: BioClinicum Research and Development Complex, Moscow, Russia. a.aleksandrova@bioclinicum.com.
authors.forename: A V
authors.initials: AV
authors.lastname: Aleksandrova
authors.affiliation: BioClinicum Research and Development Complex, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Senyavina
authors.affiliation: BioClinicum Research and Development Complex, Moscow, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Maltseva
authors.affiliation: BioClinicum Research and Development Complex, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Khutornenko
authors.affiliation: BioClinicum Research and Development Complex, Moscow, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Sakharov
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology*; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D009474:Neurons / Q000187:drug effects*; D018384:Oxidative Stress; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D018712:Analgesics, Non-Narcotic; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D000082:Acetaminophen; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: acetaminophen; cell death mechanisms; neural cells