title: Efficient Capture and Isolation of Tumor-Related Circulating Cell-Free DNA from Cancer Patients Using Electroactive Conducting Polymer Nanowire Platforms.
abstract: Circulating cell-free DNA (cfDNA) is currently recognized as a key non-invasive biomarker for cancer diagnosis and progression and therapeutic efficacy monitoring. Because cfDNA has been detected in patients with diverse types of cancers, the use of efficient strategies to isolate cfDNA not only provides valuable insights into tumour biology, but also offers the potential for developing new cancer-specific targets. However, the challenges associated with conventional cfDNA extraction methods prevent their further clinical applications. Here, we developed a nanostructured conductive polymer platform for the efficient capture and release of circulating cfDNA and demonstrated its potential clinical utility using unprocessed plasma samples from patients with breast and lung cancers. Our results confirmed that the platform's enhanced efficiency allows tumor-specific circulating cfDNA to be recovered at high yield and purity.
authors.affiliation: 1. New Experimental Therapeutic Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;; 3. Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine;
authors.forename: SeungHyun
authors.initials: S
authors.lastname: Jeon
authors.affiliation: 1. New Experimental Therapeutic Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;; 4. Department of Medical Science, Yonsei University College of Medicine, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea;
authors.forename: HyungJae
authors.initials: H
authors.lastname: Lee
authors.affiliation: 2. Lung Cancer Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;
authors.forename: Kieun
authors.initials: K
authors.lastname: Bae
authors.affiliation: 2. Lung Cancer Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;; 5. College of Veterinary Medicine, Konkuk University, Seoul, Korea.
authors.forename: Kyong-Ah
authors.initials: KA
authors.lastname: Yoon
authors.affiliation: 1. New Experimental Therapeutic Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Lee
authors.affiliation: 1. New Experimental Therapeutic Branch, National Cancer Center, Goyang, Gyeonggi-do 410-769, South Korea;
authors.forename: Youngnam
authors.initials: Y
authors.lastname: Cho
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000302:isolation & purification; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000473:pathology; D053770:Nanowires; D011108:Polymers
chemical_list: D014408:Biomarkers, Tumor; D011108:Polymers; D004247:DNA
keywords: cancer; circulating cell-free DNA; conducting polymer; liquid biopsy; mutation detection.; nanowired structure