title: Intrahepatic Xenograft of Cutaneous T-Cell Lymphoma Cell Lines: A Useful Model for Rapid Biological and Therapeutic Evaluation.
abstract: Cutaneous T-cell lymphomas (CTCLs) are a heterogeneous group of diseases primarily involving the skin that could have an aggressive course with circulating blood cells, especially in Sézary syndrome and transformed mycosis fungoides. So far, few CTCL cell lines have been adapted for in vivo experiments and their tumorigenicity has not been adequately assessed, hampering the use of a reproducible model for CTCL biological evaluation. In fact, both patient-derived xenografts and cell line xenografts at subcutaneous sites failed to provide a robust tool, because engraftment was dependent on mice strain and cell line subtype. Herein, we describe an original method of intrahepatic injection into adult NOD.Cg-Prkdc(scid)Il2rg(tm1Wjl)/SzJ mice liver of both aggressive (My-La, HUT78, HH, MAC2A, and MAC2B) and indolent (FE-PD and MAC1) CTCL cell lines. Six of the seven CTCL cell lines were grafted with a high rate of success (80%). Moreover, this model provided a quick (15 days) and robust assay for in vivo evaluation of CTCL cell lines tumorigenicity and therapeutic response in preclinical studies. Such a reproducible model can be therefore used for further functional studies and in vivo drug testing.
authors.affiliation: Cutaneous Lymphoma Oncogenesis Team, INSERM U1053 BordeAux Research in Translational Oncology, Bordeaux University, Bordeaux, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Andrique
authors.affiliation: Cutaneous Lymphoma Oncogenesis Team, INSERM U1053 BordeAux Research in Translational Oncology, Bordeaux University, Bordeaux, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Poglio
authors.affiliation: Cutaneous Lymphoma Oncogenesis Team, INSERM U1053 BordeAux Research in Translational Oncology, Bordeaux University, Bordeaux, France.
authors.forename: Martina
authors.initials: M
authors.lastname: Prochazkova-Carlotti
authors.affiliation: Department of Dermatology, Boston University and Roger Williams Medical Center, Providence, Rhode Island.
authors.forename: Marshall Edward
authors.initials: ME
authors.lastname: Kadin
authors.affiliation: Histology Platform Service Mixed Unit TransBioMed Core, Bordeaux University, Bordeaux, France.
authors.forename: Alban
authors.initials: A
authors.lastname: Giese
authors.affiliation: Cutaneous Lymphoma Oncogenesis Team, INSERM U1053 BordeAux Research in Translational Oncology, Bordeaux University, Bordeaux, France.
authors.forename: Yamina
authors.initials: Y
authors.lastname: Idrissi
authors.affiliation: Cutaneous Lymphoma Oncogenesis Team, INSERM U1053 BordeAux Research in Translational Oncology, Bordeaux University, Bordeaux, France; Department of Dermatology, University Hospital Center Bordeaux, Bordeaux, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Beylot-Barry
authors.affiliation: Cutaneous Lymphoma Oncogenesis Team, INSERM U1053 BordeAux Research in Translational Oncology, Bordeaux University, Bordeaux, France; Tumor Bank and Tumor Biology Laboratory, University Hospital Center Bordeaux, Pessac, France. Electronic address: jp.merlio@u-bordeaux.fr.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Merlio
authors.affiliation: Cutaneous Lymphoma Oncogenesis Team, INSERM U1053 BordeAux Research in Translational Oncology, Bordeaux University, Bordeaux, France.
authors.forename: Edith
authors.initials: E
authors.lastname: Chevret
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000637:transplantation*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver; D016410:Lymphoma, T-Cell, Cutaneous / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D023041:Xenograft Model Antitumor Assays / Q000379:methods*