title: AcEBP1, an ErbB3-Binding Protein (EBP1) from halophyte Atriplex canescens, negatively regulates cell growth and stress responses in Arabidopsis.
abstract: An ErbB-3-binding protein gene AcEBP1, also known as proliferation-associated 2G4 gene (PA2G4s) belonging to the M24 superfamily, was obtained from the saltbush Atriplex canescens. Subcellular localization imaging showed the fusion protein AcEBP1-eGFP was located in the nucleus of epidermal cells in Nicotiana benthamiana. The AcEBP1 gene expression levels were up-regulated under salt, osmotic stress, and hormones treatment as revealed by qRT-PCR. Overexpression of AcEBP1 in Arabidopsis demonstrated that AcEBP1 was involved in root cell growth and stress responses (NaCl, osmotic stress, ABA, low temperature, and drought). These phenotypic data were correlated with the expression patterns of stress responsive genes and PR genes. The AcEBP1 transgenic Arabidopsis plants also displayed increased sensitivity under low temperature and evaluated resistance to drought stress. Together, these results demonstrate that AcEBP1 negatively affects cell growth and is a regulator under stress conditions.
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062 Jilin, China. Electronic address: lijingtao789@126.com.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062 Jilin, China. Electronic address: chrisyu_gang@126.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Yu
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062 Jilin, China. Electronic address: xinhua_sun@126.com.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062 Jilin, China. Electronic address: zhangxianghui@jlu.edu.cn.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062 Jilin, China. Electronic address: jlliu@jlu.edu.cn.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Plant Science, Jilin University, Changchun, 130062 Jilin, China. Electronic address: panhongyu@jlu.edu.cn.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Pan
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology*; D027466:Atriplex / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D007211:Indoles; D009997:Osmotic Pressure / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D020893:Receptor, ErbB-3 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D055051:Salt-Tolerant Plants / Q000378:metabolism / Q000502:physiology*; D017421:Sequence Analysis; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D007211:Indoles; D010940:Plant Proteins; C016060:phyriaviolin; D020893:Receptor, ErbB-3
keywords: Abiotic stress; AcEBP1; Arabidopsis; Atriplex canescens; Overexpressing