title: Sphingomonas hankyongensis sp. nov. isolated from tap water.
abstract: A Gram reaction-negative, strictly aerobic, non-motile, translucent and rod-shaped bacterium (designated W1-2-4(T)) isolated from tap water was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-4(T) was observed to grow optimally at 25-30 °C and at pH 6.5 on nutrient agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W1-2-4(T) belongs to the genus Sphingomonas and is most closely related to the Sphingomonas fennica K101(T) (95.3 % similarity). The G+C content of genomic DNA was 67.1 mol%. Chemotaxonomic data [major ubiquinone-Q-10, major polyamine-homospermidine, major fatty acids-summed feature 8 (comprising C18:1 ω7c/ω6c), C16:0 and C14:0 2OH] supported the affiliation of strain W1-2-4(T) to the genus Sphingomonas. Strain W1-2-4(T) could be differentiated genotypically and phenotypically from the recognized species of the genus Sphingomonas. The novel isolate therefore represents a novel species, for which the name Sphingomonas hankyongensis sp. nov. is proposed, with the type strain W1-2-4(T) (=KACC 18308(T) = LMG 28595(T)).
authors.affiliation: Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.|Genomic Informatics Center, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.
authors.forename: Sung-Sik
authors.initials: SS
authors.lastname: Yun
authors.affiliation: Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.|Genomic Informatics Center, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.
authors.forename: Muhammad Zubair
authors.initials: MZ
authors.lastname: Siddiqi
authors.affiliation: Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.|Genomic Informatics Center, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.
authors.forename: Soon-Youl
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Konyang University, 121 Daehak-ro, Nonsan, Chungnam, Republic of Korea.
authors.forename: Minseok S
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.|Genomic Informatics Center, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea.
authors.forename: KangDuk
authors.initials: K
authors.lastname: Choi
authors.affiliation: Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea. wandra@hknu.ac.kr.|Genomic Informatics Center, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea. wandra@hknu.ac.kr.
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D060766:Drinking Water / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014451:Ubiquinone / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D060766:Drinking Water; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2
keywords: 16S rRNA sequence; Polyphasic taxonomy; Sphingomonas hankyongensis; Tap water