title: Studying the Human Microbiota.
abstract: There are a range of methodologies available to study the human microbiota, ranging from traditional approaches such as culturing through to state-of-the-art developments in next generation DNA sequencing technologies. The advent of molecular techniques in particular has opened up tremendous new avenues for research, and has galvanised interest in the study of our microbial inhabitants. Given the dazzling array of available options, however, it is important to understand the inherent advantages and limitations of each technique so that the best approach can be employed to address the particular research objective. In this chapter we cover some of the most widely used current techniques in human microbiota research and highlight the particular strengths and caveats associated with each approach.
authors.affiliation: Microbiology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK. alan.walker@abdn.ac.uk.
authors.forename: Alan W
authors.initials: AW
authors.lastname: Walker
mesh_terms: D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007553:Isotope Labeling / Q000379:methods; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*
chemical_list: D003470:Culture Media
keywords: FISH; Metabolomics; Microbiota; PCR; Proteomics; Sequencing; Stable isotope; Techniques