title: MicroRNA-217 inhibits cell proliferation and invasion by targeting Runx2 in human glioma.
abstract: MircroRNA-217 (miR-217) has been showed to involve in the initiation and development of human cancers, and is recognize as a tumor suppressor miRNA in several tumors. However, the clinical significance and its underlying role in human glioma remain unclear. Herein, we found that the expression of miR-217 was significantly down-regulated in glioma tissues as compared with adjacent normal brain tissues. Clinical association analysis disclosed that low-expression of miR-217 was evidently negative associated with advanced tumor stage (grade III + IV) in glioma. Further function assays showed that miR-217 inhibited proliferation, colony formation, invasion and migration of glioma cells. Notably, runt-related transcription factors 2 (Runx2) was identified as a functional target of miR-217 in glioma. Furthermore, an inverse correlation between miR-217 and Runx2 expression was observed in glioma tissues. Downregulation of Runx2 has similar with inhibition effect of overexpression of miR-217, and upregulation of Runx2 reversed the effects of overexpressing of miR-217. Taken together, these results suggest a critical role of miR-217 in suppressing proliferation, migration, and invasion of glioma by targeting Runx2.
authors.affiliation: Department of Radiotherapy, China-Japan Union Hospital of Jilin University Changchun130033, P.R. China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Nuclear Medicine, The First Hospital of Jilin University Changchun 130021, P.R. China.
authors.forename: Hongguang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Radiotherapy, China-Japan Union Hospital of Jilin University Changchun130033, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Neurosurgery, The First Hospital of Jilin University Changchun 130021, P.R. China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Xu
keywords: Glioma; Runx2; invasion; miR-217; proliferation