title: Quantitative proteomic analysis of milk fat globule membrane (MFGM) proteins in human and bovine colostrum and mature milk samples through iTRAQ labeling.
abstract: Milk fat globule membrane (MFGM) proteins have many functions. To explore the different proteomics of human and bovine MFGM, MFGM proteins were separated from human and bovine colostrum and mature milk, and analyzed by the iTRAQ proteomic approach. A total of 411 proteins were recognized and quantified. Among these, 232 kinds of differentially expressed proteins were identified. These differentially expressed proteins were analyzed based on multivariate analysis, gene ontology (GO) annotation and KEGG pathway. Biological processes involved were response to stimulus, localization, establishment of localization, and the immune system process. Cellular components engaged were the extracellular space, extracellular region parts, cell fractions, and vesicles. Molecular functions touched upon were protein binding, nucleotide binding, and enzyme inhibitor activity. The KEGG pathway analysis showed several pathways, including regulation of the actin cytoskeleton, focal adhesion, neurotrophin signaling pathway, leukocyte transendothelial migration, tight junction, complement and coagulation cascades, vascular endothelial growth factor signaling pathway, and adherens junction. These results enhance our understanding of different proteomes of human and bovine MFGM across different lactation phases, which could provide important information and potential directions for the infant milk powder and functional food industries.
authors.affiliation: College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China. yxqsyau@126.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Cong
authors.forename: Xiuming
authors.initials: X
authors.lastname: Peng
authors.forename: Junrui
authors.initials: J
authors.lastname: Wu
authors.forename: Rina
authors.initials: R
authors.lastname: Wu
authors.forename: Biao
authors.initials: B
authors.lastname: Liu
authors.forename: Wenhui
authors.initials: W
authors.lastname: Ye
authors.forename: Xiqing
authors.initials: X
authors.lastname: Yue
mesh_terms: D008841:Actin Cytoskeleton; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D003126:Colostrum / Q000737:chemistry* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D022001:Focal Adhesions; D055951:Functional Food; D063990:Gene Ontology; D006017:Glycolipids / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D055633:Immune System Phenomena; D007774:Lactation / Q000378:metabolism; D007962:Leukocytes; D066292:Lipid Droplets; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D008894:Milk Proteins / Q000378:metabolism; D008895:Milk, Human / Q000737:chemistry* / Q000378:metabolism; D015999:Multivariate Analysis; D009414:Nerve Growth Factors; D009711:Nucleotides; D011485:Protein Binding; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry; D019108:Tight Junctions; D057705:Transendothelial and Transepithelial Migration; D042461:Vascular Endothelial Growth Factor A
chemical_list: D004791:Enzyme Inhibitors; D006017:Glycolipids; D006023:Glycoproteins; D008565:Membrane Proteins; D008894:Milk Proteins; D009414:Nerve Growth Factors; D009711:Nucleotides; D020543:Proteome; D042461:Vascular Endothelial Growth Factor A; C110367:milk fat globule