title: Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects.
abstract: OBJECTIVE
  The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2.
  
  
  METHODS
  Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (µCT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals.
  
  
  RESULTS
  Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a two-fold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The µCT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks.
  
  
  CONCLUSIONS
  Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.
authors.affiliation: R&D Institute, Korea Tooth Bank, Seoul, Korea.
authors.forename: In-Woong
authors.identifier: 0000-0002-4628-3662
authors.initials: IW
authors.lastname: Um
authors.affiliation: Department of Medicine, Korea University Graduate School, Seoul, Korea.
authors.forename: Suk-Hyun
authors.identifier: 0000-0002-0328-8551
authors.initials: SH
authors.lastname: Hwang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Section of Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Young-Kyun
authors.identifier: 0000-0002-7268-3870
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, Cheonan, Korea.
authors.forename: Moon-Young
authors.identifier: 0000-0001-9596-7481
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Department of Dentistry, Korea University Anam Hospital, Seoul, Korea.
authors.forename: Sang-Ho
authors.identifier: 0000-0002-4243-788X
authors.initials: SH
authors.lastname: Jun
authors.affiliation: Department of Dentistry, Korea University Anam Hospital, Seoul, Korea.
authors.forename: Jae-Jun
authors.identifier: 0000-0001-6903-5955
authors.initials: JJ
authors.lastname: Ryu
authors.affiliation: Department of Dentistry, Korea University Ansan Hospital, Ansan, Korea.
authors.forename: Hyon-Seok
authors.identifier: 0000-0002-8493-4657
authors.initials: HS
authors.lastname: Jang
keywords: Demineralized dentin matrix; Histomorphometric analysis; Microcomputed tomography; Recombinant human bone morphogenetic protein-2