title: Antioxidant activity of bulbs and aerial parts of Crocus caspius, impact of extraction methods.
abstract: Crocus genus (Iridaceae) is comprises approximately 80 species. In this study in vitro antioxidant activities of extracts from C. caspius bulbs and aerial parts were investigated. Ultrasonically assisted extraction (US), percolation method (PE) and polyphenolic fraction (PP) were used. Antioxidant activities were evaluated with five different tests. Aerial parts US extract with high levels of phenol and flavonoids were the most potent extract in DPPH radical scavenging than others. Aerial parts PE extract had shown very potent reducing power, which was so better than other extracts (p<0.01). Aerial parts PP fraction showed very good Fe(2+) chelating ability. Aerial parts US extract were the most potent extract in scavenging of H(2)O(2). Bulb PP fraction with IC(50)=22.8±0.7 µg ml(-1) was the most potent fraction in nitric oxide scavenging. The results improved high levels of antioxidant activities of C. caspius bulbs and aerial parts in all tested models.
authors.affiliation: Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Masomeh
authors.initials: M
authors.lastname: Khalili
authors.affiliation: Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Fathi
authors.affiliation: Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Ebrahimzadeh
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D027622:Crocus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000737:chemistry; D007502:Iron Chelating Agents / Q000494:pharmacology; D009569:Nitric Oxide / Q000737:chemistry; D010084:Oxidation-Reduction; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D010946:Plants, Medicinal; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl