title: Grape seed and skin extract protects kidney from doxorubicin-induced oxidative injury.
abstract: The study investigated the protective effect of grape seed and skin extract (GSSE) against doxorubicin-induced renal toxicity in healthy rats. Animals were treated with GSSE or not (control), for 8 days, administered with doxorubicin (20mg/kg) in the 4th day, and renal function as well as oxidative stress parameters were evaluated. Data showed that doxorubicin induced renal toxicity by affecting renal architecture and plasma creatinine. Doxorubicin also induced an oxidative stress characterized by an increase in malondialdehyde (MDA), calcium and H(2)O(2) and a decrease in catalase (CAT) and superoxide dismutase (SOD). Unexpectedly doxorubicin increased peroxidase (POD) and decreased carbonyl protein and plasma urea. Treatment with GSSE counteracted almost all adverse effects induced by doxorubicin. Data suggest that doxorubicin induced an oxidative stress into rat kidney and GSSE exerted antioxidant properties, which seem to be mediated by the modulation of intracellular calcium.
authors.affiliation: Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis, Campus Universitaire El Manar II-2092 Tunis, Tunisie.
authors.forename: Meherzia
authors.initials: M
authors.lastname: Mokni
authors.affiliation: Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis, Campus Universitaire El Manar II-2092 Tunis, Tunisie.
authors.forename: Sonia
authors.initials: S
authors.lastname: Hamlaoui
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie.
authors.forename: Safwen
authors.initials: S
authors.lastname: Kadri
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie.
authors.forename: Ferid
authors.initials: F
authors.lastname: Limam
authors.affiliation: Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis, Campus Universitaire El Manar II-2092 Tunis, Tunisie.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Amri
authors.affiliation: Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis, Campus Universitaire El Manar II-2092 Tunis, Tunisie.
authors.forename: Lamjed
authors.initials: L
authors.lastname: Marzouki
authors.affiliation: Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie.
authors.forename: Ezzedine
authors.initials: E
authors.lastname: Aouani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D002118:Calcium / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D019610:Cytoprotection; D004195:Disease Models, Animal; D004317:Doxorubicin; D005260:Female; D005638:Fruit; D056604:Grape Seed Extract / Q000302:isolation & purification / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010946:Plants, Medicinal; D050050:Protein Carbonylation / Q000187:drug effects; D017208:Rats, Wistar; D013997:Time Factors; D014508:Urea / Q000097:blood; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D056604:Grape Seed Extract; D004317:Doxorubicin; D014508:Urea; D003404:Creatinine; D002118:Calcium