title: [Construction of Nude Mouse Model of Triple Negative Breast Cancer and Value of Thermal Tomography].
abstract: OBJECTIVE
  To explore the early detection of breast cancer by ultrasonic imaging and thermal tomography of luciferase or green fluorescent protein (GFP)-labeled MDA-MB-231 breast cancer cell line-xenografts in nude mice.
  
  
  METHODS
  Fluorescence-tagged lentiviral vectors were transfected into the triple-negative breast cancer cell line MDA-MB-231. These cells were implanted either subcutaneously under the right breast pad or intravenously into the tail vein of nude BALB/C mice. Thermal tomography and ultrasound imaging were used to detect tumor formation and to monitor tumor growth and metastasis in vivo.
  
  
  RESULTS
  Triple negative breast cancer cell line-xenografts were used to successfully construct an orthotopic nude mice model of breast cancer metastasis in the peritoneum. Thermal tomography and ultrasound imaging were used together to detect small tumors. Thermal tomography imaging detected small tumors earlier than ultrasound imaging.
  
  
  CONCLUSIONS
  Thermal tomography can be used to monitor changes in tumor growth and detect abnormal tissue. Therefore, it can serve as a convenient,rapid,sensitive, and reliable technique for early screening of human breast cancer.
authors.affiliation: Department of South Building Ultrasound,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Huo
authors.affiliation: Department of Health,Inner Mongolia Bayinnaoer City Linhe People's Hospital,Bayannur,Inner Mongolia 015000,China.
authors.forename: Jian-bin
authors.initials: JB
authors.lastname: Huo
authors.affiliation: Department of South Building Ultrasound,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Wan
authors.affiliation: Department of South Building Ultrasound,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Jun-lai
authors.initials: JL
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014057:Tomography, X-Ray Computed; D064726:Triple Negative Breast Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000473:pathology; D014463:Ultrasonography