title: Type I collagen and its daughter peptides for targeting mucosal healing in ulcerative colitis: A new treatment strategy.
abstract: Ulcerative colitis, particularly the chronic persistent form is characterized by the presence of active inflammation and extensive areas of ulceration in the colonic mucosa. The existing treatment protocol aims at only reducing intestinal inflammation, rather than targeting mucosal ulceration. In this study, type I collagen and its daughter peptides called collagen hydrolysate, highly popular reconstructive materials for tissue engineering applications, are hypothesized as healing matrices to target the recuperation of internal mucosal ulceration. The clinical assessments on day 10 of dextran sodium sulfate induced colitis in mice model revealed that both the collagen (1.56±0.29) and collagen hydrolysate treatments (1.33±0.33) showed a significant reduction in the rectal bleeding compared to the reference mesalamine treatment (2.50±0.33) and untreated negative control (2.40±0.40). VEGF, a potent angiogenic growth factor, over expressed during UC was down-regulated by collagen hydrolysate (1.06±0.25) and collagen (1.76±0.45) to a greater extent than by mesalamine (2.59±0.51) and untreated control (4.17±0.15). The down-regulation of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6 also follows the same pattern. Histological observations were in accordance with the clinical indicators. Both collagen and collagen hydrolysate treatments showed significant reduction in mucosal damage score and facilitated faster regeneration of damaged mucosa.
authors.affiliation: Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai, Tamil Nadu, India.
authors.forename: Satiesh Kumar
authors.initials: SK
authors.lastname: Ramadass
authors.affiliation: Siddha Central Research Institute, Central Council for Research in Siddha, Govt. Anna Hospital Campus, Arumbakkam, Chennai 600106, India.
authors.forename: Sugin Lal
authors.initials: SL
authors.lastname: Jabaris
authors.affiliation: Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai, Tamil Nadu, India.
authors.forename: Ramesh Kannan
authors.initials: RK
authors.lastname: Perumal
authors.affiliation: Pondicherry Centre for Biological Sciences, Pondicherry, India.
authors.forename: Villianur Ibrahim
authors.initials: VI
authors.lastname: HairulIslam
authors.affiliation: Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai, Tamil Nadu, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Gopinath
authors.affiliation: Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai, Tamil Nadu, India. Electronic address: madhan@clri.res.in.
authors.forename: Balaraman
authors.initials: B
authors.lastname: Madhan
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D024042:Collagen Type I / Q000494:pharmacology / Q000627:therapeutic use*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000235:genetics; D016264:Dextran Sulfate; D016503:Drug Delivery Systems; D005346:Fibroblast Growth Factors / Q000235:genetics; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D024042:Collagen Type I; D016207:Cytokines; D010455:Peptides; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D005346:Fibroblast Growth Factors; D016264:Dextran Sulfate
keywords: Collagen; Collagen hydrolysate; Mucosal damage; Ulcerative colitis