title: Breviscapine attenuatted contrast medium-induced nephropathy via PKC/Akt/MAPK signalling in diabetic mice.
abstract: Contrast medium-induced nephropathy (CIN) remains a major cause of iatrogenic, drug-induced renal injury. Recent studies reveal that Breviscapine can ameliorate diabetic nephropathy in mice. Yet it remains unknown if Breviscapine could reduce CIN in diabetic mice. In this study, male C57/BL6J mice were randomly divided into 7 groups: control, diabetes mellitus, CIN, diabetes mellitus+CIN, diabetes mellitus+Breviscapine, CIN+Breviscapine and diabetes mellitus+CIN+Breviscapine. Model of CIN was induced by tail intravenous administration of iopromide and model of diabetes mellitus was induced by Streptozotocin intraperitoneally. Breviscapine was administered intragastrically for 4 weeks. Renal function parameters, kidney histology, markers of renal fibrosis, phosphorylation of protein kinase C/Akt/mitogen activated protein kinases were measured by western blot. We found out that diabetes mellitus aggravated CIN damage. Renal histological analysis showed Breviscapine reduced of renal fibrosis and tubular damage. Breviscapine was also shown markedly to ameliorate CIN fibrotic markers expression, reduced proteinuria and serum creatinine. Furthermore, Breviscapine decreased phosphorylation of PKCβII, Akt, JNK1/2 and p38. Therefore, Breviscapine treatment could ameliorate the development of CIN in diabetic mice, which was partly attributed to its suppression of renal fibrosis via phosphorylation of PKCβII/Akt/JNK1/2/p38 signalling.
authors.affiliation: Department of Cardiology, The Third Clinical Institute Affiliated To Wenzhou Medical University No. 57 Canghou Street, Wenzhou 325000, Zhejiang Province, PR China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University No. 3 East Qingchun Road, Hangzhou 310016, Zhejiang Province, PR China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Third Clinical Institute Affiliated To Wenzhou Medical University No. 57 Canghou Street, Wenzhou 325000, Zhejiang Province, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The Third Clinical Institute Affiliated To Wenzhou Medical University No. 57 Canghou Street, Wenzhou 325000, Zhejiang Province, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Cardiology, The Third Clinical Institute Affiliated To Wenzhou Medical University No. 57 Canghou Street, Wenzhou 325000, Zhejiang Province, PR China.
authors.forename: Youyang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Cardiology, The Third Clinical Institute Affiliated To Wenzhou Medical University No. 57 Canghou Street, Wenzhou 325000, Zhejiang Province, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University No. 3 East Qingchun Road, Hangzhou 310016, Zhejiang Province, PR China.
authors.forename: Zhida
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University No. 3 East Qingchun Road, Hangzhou 310016, Zhejiang Province, PR China.
authors.forename: Jialin
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University No. 3 East Qingchun Road, Hangzhou 310016, Zhejiang Province, PR China.
authors.forename: Shengyu
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University No. 3 East Qingchun Road, Hangzhou 310016, Zhejiang Province, PR China.
authors.forename: Jiefang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University No. 3 East Qingchun Road, Hangzhou 310016, Zhejiang Province, PR China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Fu
keywords: Contrast medium-induced nephropathy; PKC/Akt/MAPK signalling; breviscapine; diabetes mellitus