title: Regulation of Vibrio mimicus metalloprotease (VMP) production by the quorum-sensing master regulatory protein, LuxR.
abstract: Vibrio mimicus is an estuarine bacterium, while it can cause severe diarrhea, wound infection, and otitis media in humans. This pathogen secretes a relatively important toxin named V. mimicus metalloprotease (VMP). In this study, we clarified regulation of the VMP production according to the quorum-sensing master regulatory protein named LuxR. First, the full length of luxR gene, encoding LuxR, was detected in V. mimicus strain E-37, an environmental isolate. Next, the putative consensus binding sequence of LuxR protein could be detected in the upstream (promoter) region of VMP encoding gene, vmp. Finally, the effect of disruption of luxR gene on the expression of vmp and production of VMP was evaluated. Namely, the expression of vmp was significantly diminished by luxR disruption and the production of VMP was severely altered. Taken together, here we report that VMP production is under the positive regulation of the quorum-sensing master regulatory protein, LuxR.
authors.affiliation: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-Naka, Kita-Ku, Okayama, Japan.|Chemical Industries Development Company, Assiut, Egypt.
authors.forename: El-Shaymaa
authors.initials: ES
authors.lastname: Abdel-Sattar
authors.affiliation: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-Naka, Kita-Ku, Okayama, Japan.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Miyoshi
authors.affiliation: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-Naka, Kita-Ku, Okayama, Japan. elgamel3a@mans.edu.eg.|Faculty of Pharmacy, Department of Microbiology and Immunology, Mansoura University, Elgomhouria Street, Mansoura, Egypt. elgamel3a@mans.edu.eg.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Elgaml
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D045726:Metalloproteases / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D053038:Quorum Sensing / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D044164:Vibrio mimicus / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012097:Repressor Proteins; D015534:Trans-Activators; C064778:LuxR autoinducer binding proteins; D045726:Metalloproteases
keywords: LuxR protein; Metalloprotease; Quorum-sensing; Vibrio mimicus