title: Thermoresponsive poly[tri(ethylene glycol) monoethyl ether methacrylate]-peptide surfaces obtained by radiation grafting-synthesis and characterisation.
abstract: This report demonstrates the feasibility of radiation grafting for the preparation of polymer layers functionalised with short peptide ligands which promote cell adhesion. Thermoresponsive poly [tri(ethylene glycol) monoethyl ether methacrylate] (PTEGMA) layers were synthesised on a polypropylene substrate by post-irradiation grafting. A cell adhesion moiety, the CF-IKVAVK peptide modified with a methacrylamide function and a fluorescent label were introduced to the surface during the polymerisation process. The amount of CF-IKVAVK was easily controlled by changing its concentration in the reaction mixture. The changes in the surface composition, morphology, philicity and thickness at each step of the polypropylene functionalisation confirmed that the surface modification procedures were successful. The increase in environmental temperature above the cloud point temperature of PTEGMA caused a decrease in surface philicity. The obtained PTEGMA and PTEGMA-peptide surfaces above TCP were tested as scaffolds for fibroblast sheet culture and temperature induced detachment.
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Wróblewskiego 15, 93-590 Lodz, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Adamus
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Wróblewskiego 15, 93-590 Lodz, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Komasa
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Wróblewskiego 15, 93-590 Lodz, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Kadłubowski
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Wróblewskiego 15, 93-590 Lodz, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Ulański
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Wróblewskiego 15, 93-590 Lodz, Poland.
authors.forename: J M
authors.initials: JM
authors.lastname: Rosiak
authors.affiliation: Center for Burn Treatment, Jana Pawla II, 41-100 Siemianowice Slaskie, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kawecki
authors.affiliation: Center for Burn Treatment, Jana Pawla II, 41-100 Siemianowice Slaskie, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Klama-Baryła
authors.affiliation: Centre of Polymer and Carbon Materials of the Polish Academy of Sciences, M. Curie-Skłodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dworak
authors.affiliation: Centre of Polymer and Carbon Materials of the Polish Academy of Sciences, M. Curie-Skłodowskiej 34, 41-819 Zabrze, Poland. Electronic address: btrzebicka@cmpw-pan.edu.pl.
authors.forename: B
authors.initials: B
authors.lastname: Trzebicka
authors.affiliation: Centre of Polymer and Carbon Materials of the Polish Academy of Sciences, M. Curie-Skłodowskiej 34, 41-819 Zabrze, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Szweda
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry; D011827:Radiation; D012867:Skin / Q000166:cytology; D060327:Solid-Phase Synthesis Techniques; D021241:Spectrometry, Mass, Electrospray Ionization; D018629:Spectrometry, Mass, Secondary Ion; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature
chemical_list: D008689:Methacrylates; D010455:Peptides; D011109:Polymethacrylic Acids; C000614246:poly(tri(ethylene glycol) monoethyl ether methacrylate); D011092:Polyethylene Glycols
keywords: IKVAV; Peptides promoting cells' adhesion; Poly[tri(ethylene glycol)monoethyl ether methacrylate]; Radiation grafting; Surface modification; Thermoresponsive surfaces