title: Analysis of the causes of discrepancies in troponin I concentrations as measured by ARCHITECT High-Sensitive Troponin I ST and STACIA CLEIA cTnI.
abstract: Background Recently developed reagents for the highly sensitive measurement of cardiac troponin I are useful for early diagnosis of acute coronary syndrome. However, differences in measured values between these new reagents and previously used reagents have not been well studied. In this study, we aimed to compare the values between ARCHITECT High-Sensitive Troponin I ST (newly developed reagents), ARCHITECT Troponin I ST and STACIA CLEIA cardiac troponin I (two previously developed reagent kits). Methods Gel filtration high-performance liquid chromatography was used to analyse the causes of differences in measured values. Results The measured values differed between ARCHITECT High-Sensitive Troponin I ST and STACIA CLEIA cardiac troponin I reagents (r = 0.82). Cross-reactivity tests using plasma with added skeletal-muscle troponin I resulted in higher reactivity (2.17-3.03%) for the STACIA CLEIA cardiac troponin I reagents compared with that for the ARCHITECT High-Sensitive Troponin I ST reagents (less than 0.014%). In addition, analysis of three representative samples using gel filtration high-performance liquid chromatography revealed reagent-specific differences in the reactivity against each cardiac troponin I complex; this could explain the differences in values observed for some of the samples. Conclusion The newly developed ARCHITECT High-Sensitive Troponin I ST reagents were not affected by the presence of skeletal-muscle troponin I in the blood and may be useful for routine examinations.
authors.affiliation: 1 Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.affiliation: 1 Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.|2 Department of Infection Control and Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.affiliation: 1 Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Mochizuki
authors.affiliation: 1 Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Asanuma
authors.affiliation: 1 Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.|2 Department of Infection Control and Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood / Q000175:diagnosis*; D057205:Automation, Laboratory; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D042241:Early Diagnosis; D006801:Humans; D018482:Muscle, Skeletal / Q000737:chemistry; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D019210:Troponin I / Q000097:blood*
chemical_list: D011933:Reagent Kits, Diagnostic; D019210:Troponin I
keywords: ARCHITECT; STACIA; Troponin I; cross-reactivity; dissociation; troponin complex