title: Microwell arrays reveal cellular heterogeneity during the clonal expansion of transformed human cells.
abstract: We developed micromolded microwell arrays to study the proliferation and senescence of single cells. Microwell arrays were designed to be compatible with conventional cell culture protocols to simplify cell loading, cell culture, and imaging. We demonstrated the utility of these arrays by measuring the proliferation and senescence of isogenic cells which expressed or had been depleted of the human Werner syndrome protein. Our results allowed us to reveal cell-to-cell heterogeneity in proliferation in WRN+ and WRN-depleted fibroblasts during clonal growth.
authors.affiliation: Departments of Bioengineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Tim C
authors.initials: TC
authors.lastname: Chang
authors.affiliation: Departments of Pathology, University of Washington, Seattle, WA 98195, USA.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Tang
authors.affiliation: Departments of Biostatistics, University of Washington, Seattle, WA 98195, USA.
authors.forename: William Jen Hoe
authors.initials: WJ
authors.lastname: Koh
authors.affiliation: Departments of Bioengineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Alexander J E
authors.initials: AJ
authors.lastname: Rettie
authors.affiliation: Departments of Biostatistics, University of Washington, Seattle, WA 98195, USA.
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Emond
authors.affiliation: Departments of Pathology, University of Washington, Seattle, WA 98195, USA; Departments of Genome Sciences, University of Washington, Seattle, WA 98195, USA.
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Monnat
authors.affiliation: Departments of Bioengineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Albert
authors.initials: A
authors.lastname: Folch