title: MiR-186 inhibited aerobic glycolysis in gastric cancer via HIF-1α regulation.
abstract: Deregulation of microRNAs in human malignancies has been well documented, among which microRNA-186 (miR-186) has an antiproliferative role in some carcinomas. Here we demonstrate that low expression of miR-186 facilitates aerobic glycolysis in gastric cancer. MiR-186 suppresses cell proliferation induced by hypoxia inducible factor 1 alpha (HIF-1α) in gastric cancer cell lines MKN45 and SGC7901. Cellular glycolysis, including cellular glucose uptake, lactate, ATP/ADP and NAD+/NADH ratios, are also inhibited by miR-186. The negative regulation of miR-186 on HIF-1α effects its downstream targets, including programmed death ligand 1 and two glycolytic key enzymes, hexokinase 2 and platelet-type phosphofructokinase. The antioncogenic effects of miR-186 are proved by in vivo xenograft tumor experiment. The results demonstrate that the miR-186/HIF-1α axis has an antioncogenic role in gastric cancer.
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The Fourth Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Fourth Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: R
authors.initials: R
authors.lastname: Bai
authors.affiliation: Department of General Surgery, The Fourth Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: K
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Tian