title: The Wound Healing Effects of Adiponectin Eye Drops after Corneal Alkali Burn.
abstract: To investigate the wound healing effect of adiponectin eye drops following corneal alkali burn.
  
  
  
  A chemical burn was induced using 0.1 M NaOH in both adenovirus 12-SV40 hybrid-transformed human corneal epithelial (HCE-2) cells and C57BL/6 mice. The injured HCE-2 and mice were then treated using either 0.1% hyaluronic acid (HA) or adiponectin at 0.0001%, 0.001%, or 0.01% concentration. The viability of the HCE-2 cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The wound healing rate of the HCE-2 cells was evaluated using a migration assay 4, 8, 12, 24, and 48 h after chemical injury. In mice, corneal epithelial defects and degree of haze were analyzed 6 h and 1, 2, 3, 6, and 7 days after chemical injury. Seven days after injury, the concentrations of IL-1β and transforming growth factor-β (TGF-β) in the cornea were measured using enzyme-linked immunosorbent assay, and histological analysis was also performed.
  
  
  
  The viability of HCE-2 cells was not affected by adiponectin at any of the concentrations used. In HCE-2 cells treated using either 0.001% or 0.01% adiponectin, the wound healing rate after 4 h was significantly faster than in the control and HA-treated groups. With regard to mice, the 0.001% and 0.01% adiponectin-treated groups showed a significant improvement in epithelial defect parameters and haze scores at 3, 5, and 7 days after chemical injury. A significant decrease in IL-1β and TGF-β levels was observed in the 0.001% and 0.01% adiponectin-treated groups compared to the other groups. Histologically, corneal thickness and the inflammatory cells were also decreased in the adiponectin-treated groups.
  
  
  
  Topical adiponectin (both 0.001% and 0.01%) increased epithelial migration and improved clinical signs and inflammation on the ocular surface after alkali burn, suggesting that adiponectin can promote wound healing in the cornea.
authors.affiliation: a Xiamen Eye Center of Xiamen University , Xiamen , Fujian , China.|b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.
authors.forename: Zhengri
authors.initials: Z
authors.lastname: Li
authors.affiliation: b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.
authors.forename: Lian
authors.initials: L
authors.lastname: Cui
authors.affiliation: b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.
authors.forename: Jee Myung
authors.initials: JM
authors.lastname: Yang
authors.affiliation: b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.
authors.forename: Hyo Seok
authors.initials: HS
authors.lastname: Lee
authors.affiliation: b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.
authors.forename: Ji Suk
authors.initials: JS
authors.lastname: Choi
authors.affiliation: c Department of Ophthalmology , Ulsan University Hospital, University of Ulsan College of Medicine , Ulsan , South Korea.
authors.forename: Je Moon
authors.initials: JM
authors.lastname: Woo
authors.affiliation: d Microbiology and Functionality Research Group, R & D Division , World Institute of Kimchi , Gwangju , South Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Lim
authors.affiliation: b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.
authors.forename: Kyung Chul
authors.initials: KC
authors.lastname: Yoon
mesh_terms: D052242:Adiponectin / Q000008:administration & dosage*; D000468:Alkalies / Q000633:toxicity; D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy* / Q000473:pathology; D002452:Cell Count; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects* / Q000473:pathology; D065306:Corneal Injuries / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D005126:Eye Burns / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009883:Ophthalmic Solutions; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D052242:Adiponectin; D000468:Alkalies; D009883:Ophthalmic Solutions
keywords: Adiponectin; corneal alkali burn; corneal epithelial cells; inflammation; wound healing