title: Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies.
abstract: OBJECTIVE
  The aim of this study was to evaluate radiofrequency-induced dextran-coated lanthanum strontium manganese oxide nanoparticles-mediated hyperthermia to be used for tumor regression in mice.
  
  
  METHODS
  Nanoparticles were injected intra-tumorally in melanoma-bearing C57BL/6J mice and were subjected to radiofrequency treatment.
  
  
  RESULTS
  Hyperthermia treatment significantly inhibited tumor growth (~84%), increased survival (~50%), and reduced tumor proliferation in mice. Histopathological examination demonstrated immense cell death in treated tumors. DNA fragmentation, increased terminal deoxynucleotidyl transferase-dUTP nick end labeling signal, and elevated levels of caspase-3 and caspase-6 suggested apoptotic cell death. Enhanced catalase activity suggested reactive oxygen species-mediated cell death. Enhanced expression of heat shock proteins 70 and 90 in treated tumors suggested the possible development of "antitumor immunity".
  
  
  CONCLUSIONS
  The dextran-coated lanthanum strontium manganese oxide-mediated hyperthermia can be used for the treatment of cancer.
authors.affiliation: Centre for Nanobioscience, Agharkar Research Institute, Pune, India.
authors.forename: Reihaneh
authors.initials: R
authors.lastname: Haghniaz
authors.affiliation: Centre for Nanobioscience, Agharkar Research Institute, Pune, India.
authors.forename: Rinku D
authors.initials: RD
authors.lastname: Umrani
authors.affiliation: Centre for Nanobioscience, Agharkar Research Institute, Pune, India.
authors.forename: Kishore M
authors.initials: KM
authors.lastname: Paknikar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation; D003911:Dextrans / Q000737:chemistry*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D006979:Hyperthermia, Induced; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000473:pathology; D007811:Lanthanum / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D017895:Manganese Compounds / Q000737:chemistry*; D008546:Melanoma, Experimental / Q000473:pathology / Q000628:therapy; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D013324:Strontium / Q000737:chemistry*; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D000975:Antioxidants; D003911:Dextrans; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D017895:Manganese Compounds; D010087:Oxides; C103829:lanthanum oxide; C027424:manganese oxide; D007811:Lanthanum; D053148:Caspase 3; D020169:Caspases; D013324:Strontium
keywords: MRI; heat shock proteins; lanthanum strontium manganese oxide nanoparticles; melanoma; survival; tumor regression