title: An ATP Aptasensor Based on the Peroxidase-like Activity of Hemin/Graphene Oxide Nanosheets.
abstract: In the present work, a sensitive electrochemical aptasensor was designed for the detection of adenosine triphosphate (ATP) with hemin/graphene oxide nanosheets (HGNs). Firstly, the ATP aptamer was self-assembled on gold electrode surface, and then HGNs were captured to the modified electrode by π-π stacking. The captured HGNs could catalyze the disproportionation reaction of H2O2, and produced a detectable electrochemical signal by chronoamperometry. ATP was competitively bound to aptamer which led to the release of HGNs from the electrode surface after adding ATP. The decrease of the electrochemical signal, which was calculated by the difference of amperometric responses before and after incubation of ATP, provided a quantitative signal for ATP detection. A linear correlation was achieved between the difference of the amperometric responses and the logarithmic concentration of ATP ranging from 0.5 to 100 nM with a detection limit of 0.08 nM. Besides, the aptasensor also exhibited good selectivity toward ATP against other analogs.
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Tao
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Lu
authors.forename: Jianmeng
authors.initials: J
authors.lastname: Zhu
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Bai
authors.forename: Jinyu
authors.initials: J
authors.lastname: Meng
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lu
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006427:Hemin / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D036103:Nanotechnology; D010087:Oxides / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D052157:Aptamers, Nucleotide; D010087:Oxides; D006427:Hemin; D006046:Gold; D006108:Graphite; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide