title: Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser.
abstract: The use of small particles has expanded the capability of ultrashort pulsed laser optoinjection technology toward simultaneous treatment of multiple cells. The microfluidic platform is one of the attractive systems that has obtained synergy with laser-based technology for cell manipulation, including optoinjection. We have demonstrated the delivery of molecules into suspended-flowing cells in a microfluidic channel by using biodegradable polymer microspheres and a near-infrared femtosecond laser pulse. The use of polylactic-co-glycolic acid microspheres realized not only a higher optoinjection ratio compared to that with polylactic acid microspheres but also avoids optical damage to the microfluidic chip, which is attributable to its higher optical intensity enhancement at the localized spot under a microsphere. Interestingly, optoinjection ratios to nucleus showed a difference for adhered cells and suspended cells. The use of biodegradable polymer microspheres provides high throughput optoinjection; i.e., multiple cells can be treated in a short time, which is promising for various applications in cell analysis, drug delivery, and ex vivo gene transfection to bone marrow cells and stem cells without concerns about residual microspheres.
authors.affiliation: Keio University, Department of Electronics and Electrical Engineering, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Ishii
authors.affiliation: Keio University, Department of Electronics and Electrical Engineering, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Ariyasu
authors.affiliation: Keio University, Department of Electronics and Electrical Engineering, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Mitsuhashi
authors.affiliation: Laser Zentrum Hannover e.V., Biomedical Optics Department, Hollerithallee 8, Hannover D- 30419, Germany.
authors.forename: Dag
authors.initials: D
authors.lastname: Heinemann
authors.affiliation: Laser Zentrum Hannover e.V., Biomedical Optics Department, Hollerithallee 8, Hannover D- 30419, GermanycGottfried Wilhelm Leibniz University Hannover, Institute of Quantum Optics, Am Welfengarten 1, Hannover 30167, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Heisterkamp
authors.affiliation: Keio University, Department of Electronics and Electrical Engineering, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Terakawa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D011108:Polymers
chemical_list: D011108:Polymers