title: Sustained Anti-inflammatory Effect of Resveratrol-Phospholipid Complex Embedded Polymeric Patch.
abstract: Resveratrol-phospholipid complex (Phytosome®) (RSVP) was found better aqueous soluble and permeable than free resveratrol (RSV). RSVPs were incorporated in polymeric patch prepared by solvent casting method using Eudragit RL 100, PVP K30, and PEG 400 for application on dermal sites for sustained treating of inflammation. Prepared patches were evaluated for various physicochemical properties, surface morphology by SEM, TEM, and compatibility of patch components by FT-IR and DSC studies. Optimized formulation (F9) gave 95.79 ± 3.02% drug release and 51.36% (4.28 ± 0.48 mg/cm2) skin permeation after 24 h. Skin extract when examined for drug accumulation showed 38.31 ± 2.42% drug content. FE-SEM images of the patch taken after drug release and skin permeation studies showed that RSVPs in polymeric patch are stable and retain their structure after 24 h long exposure to physiologic environment. Sustained anti-inflammatory effect was established in carrageenan-induced paw edema model in which test formulation gave 84.10% inhibition of inflammation at 24 h as compared to 39.58% for standard diclofenac sodium gel. The CLSM study confirmed the localization of RSVPs for a longer period, thus enabling drug targeting to the dermis for sustained effect. Skin irritation test on rabbit revealed that the patches are safe for skin application. Histological observations suggested that after exposure to the permeants, the SC integrity had not altered and no evidence of presence of inflammatory cells found. RSVP (Phytosome®) containing patches abled to give sustained therapeutic effect that may be useful in treating acute and chronic inflammation.
authors.affiliation: Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, 781017, India. bhupenkalita7@gmail.com.|Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, 786004, India. bhupenkalita7@gmail.com.
authors.forename: Bhupen
authors.initials: B
authors.lastname: Kalita
authors.affiliation: Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, 786004, India.
authors.forename: Malay K
authors.initials: MK
authors.lastname: Das
authors.affiliation: Department of Anatomy and Histology, College of Veterinary Science, Guwahati, 781022, India.
authors.forename: Munmun
authors.initials: M
authors.lastname: Sarma
authors.affiliation: Department of Anatomy and Histology, College of Veterinary Science, Guwahati, 781022, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Deka
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000279:Administration, Cutaneous; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D002351:Carrageenan / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D010539:Permeability; D010743:Phospholipids / Q000008:administration & dosage* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects; D013267:Stilbenes / Q000008:administration & dosage* / Q000737:chemistry; D057968:Transdermal Patch
chemical_list: D000180:Acrylic Resins; D000893:Anti-Inflammatory Agents; D003692:Delayed-Action Preparations; D010743:Phospholipids; D011108:Polymers; D013267:Stilbenes; C050528:Eudragit RS; D011092:Polyethylene Glycols; D002351:Carrageenan; D000077185:Resveratrol
keywords: anti-inflammatory; patch; phospholipid complex; phytosome®; resveratrol