title: Flavonoid profile and antileukemic activity of Coreopsis lanceolata flowers.
abstract: Coreopsis lanceolata is a perennial plant belonging to the Asteraceae family. In this study, flavonoid profile and antileukemic potential of yellow flowers of the plant were investigated. The total flavonoid content in EtOAc fraction of the flower methanol extract was found to be 420mg/g and showed the inhibition of cell proliferation and possible induction of apoptosis in human leukemia HL-60 cells. Our phytochemical research led to the isolation of rare flavonoids including a flavanone, chalcones, and aurones; in particular, 4-methoxylanceoletin demonstrated the potent antiproliferative activity. Comparison with other Asteraceaeous flowers by UPLC-MS analysis indicated that the isolates are characteristic constituents of C. lanceolata.
authors.affiliation: Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Chemistry Education, Islamic University of Kalimantan, Jl. Adhyaksa No. 2 Kayu Tangi, Banjarmasin 70123, Indonesia.
authors.forename: Antoni
authors.initials: A
authors.lastname: Pardede
authors.affiliation: Department of Materials Science and Technology, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Koharu
authors.initials: K
authors.lastname: Mashita
authors.affiliation: Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Materials Science and Technology, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ninomiya
authors.affiliation: Division of Anaerobe Research, Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan; United Graduate School of Drug Discovery and Medicinal Information Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Materials Science and Technology, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: koketsu@gifu-u.ac.jp.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Koketsu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D031945:Coreopsis / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids
keywords: 4-Methoxylanceoletin; Antileukemic activity; Aurone; Chalcone; Coreopsis lanceolata