title: A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue.
abstract: A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.
authors.affiliation: Centre for Translational Omics, UCL Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK. Electronic address: wendy.heywood@ucl.ac.uk.
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Heywood
authors.affiliation: Histopathology Unit, Great Ormond Street Hospital for Children, Great Ormond Street, London, WC1N 3JH, UK.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Pryce
authors.affiliation: Histopathology Unit, Great Ormond Street Hospital for Children, Great Ormond Street, London, WC1N 3JH, UK.
authors.forename: Alex
authors.initials: A
authors.lastname: Virasami
authors.affiliation: Centre for Translational Omics, UCL Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK; Histopathology Unit, Great Ormond Street Hospital for Children, Great Ormond Street, London, WC1N 3JH, UK.
authors.forename: Rhian Lauren
authors.initials: RL
authors.lastname: Preece
authors.affiliation: Life Course Epidemiology and Biostatistics, UCL Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK.
authors.forename: Carol
authors.initials: C
authors.lastname: Dezateux
authors.affiliation: Centre for Translational Omics, UCL Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Mills
authors.affiliation: Histopathology Unit, Great Ormond Street Hospital for Children, Great Ormond Street, London, WC1N 3JH, UK.
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Sebire
mesh_terms: D002853:Chromatography, Liquid; D036861:Delivery, Obstetric; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*; D013048:Specimen Handling
keywords: Free peptide; LC-MS; Multivariate analysis; Placenta; Proteolysis