title: In vitro angiotensin I converting enzyme inhibition by a peptide isolated from Chiropsalmus quadrigatus Haeckel (box jellyfish) venom hydrolysate.
abstract: The anti-angiotensin I converting enzyme activity of box jellyfish, Chiropsalmus quadrigatus Haeckel venom hydrolysate was studied. The venom extract was obtained by centrifugation and ultrasonication. Protein concentration of 12.99 μg/mL was determined using Bradford assay. The pepsin and papain hydrolysate was tested for its toxicity by Limit test following the OECD Guideline 425 using 5 female Sprague-Dawley rats. Results showed that the hydrolysate is nontoxic with an LD50 above 2000 mg/kg. In vitro angiotensin I converting enzyme (ACE) inhibitory activity was determined using ACE kit-WST. Isolation of ACE inhibitory peptides using column chromatography with SP-Sephadex G-25 yielded 8 pooled fractions with fraction 3 (86.5%) exhibiting the highest activity. This was followed by reverse phase - high performance liquid chromatography (RP-HPLC) with an octadecyl silica column (Inertsil ODS-3) using methanol:water 15:85 at a flow rate of 1.0 mL/min. Among the 13 fractions separated with the RP-HPLC, fraction 3.5 exhibited the highest ACE inhibitory activity (84.1%). The peptide sequence ACPGPNPGRP (IC50 2.03 μM) from fraction 3.5 was identified using Matrix-assisted laser desorption/ionization with time-of-flight tandem mass spectroscopy analysis (MALDI-TOF/MS).
authors.affiliation: The Graduate School, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; College of Nursing, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines. Electronic address: sopamela23@gmail.com.
authors.forename: Pamela Berilyn T
authors.initials: PB
authors.lastname: So
authors.affiliation: The Graduate School, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; Research Center for the Natural and Applied Sciences, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines.
authors.forename: Peter
authors.initials: P
authors.lastname: Rubio
authors.affiliation: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li, 320, Taiwan.
authors.forename: Stephen
authors.initials: S
authors.lastname: Lirio
authors.affiliation: The Graduate School, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; Research Center for the Natural and Applied Sciences, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; College of Science, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines.
authors.forename: Allan Patrick
authors.initials: AP
authors.lastname: Macabeo
authors.affiliation: Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li, 320, Taiwan.
authors.forename: Hsi-Ya
authors.initials: HY
authors.lastname: Huang
authors.affiliation: The Graduate School, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; Research Center for the Natural and Applied Sciences, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; Faculty of Pharmacy, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines.
authors.forename: Mary Jho-Anne T
authors.initials: MJ
authors.lastname: Corpuz
authors.affiliation: The Graduate School, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; Research Center for the Natural and Applied Sciences, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines; College of Nursing, University of Santo Tomas, España Boulevard, 1015, Manila, Philippines. Electronic address: oliver_7592@yahoo.com.
authors.forename: Oliver B
authors.initials: OB
authors.lastname: Villaflores
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D003064:Cnidarian Venoms / Q000737:chemistry*; D005260:Female; D006868:Hydrolysis; D066298:In Vitro Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D003064:Cnidarian Venoms
keywords: ACE; Box jellyfish; Chiropsalmus quadrigatus; Hypertension; Venom