title: Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat.
abstract: Targeted sequence capture is a promising technology which helps reduce costs for sequencing and genotyping numerous genomic regions in large sets of individuals. Bait sequences are designed to capture specific alleles previously discovered in parents or reference populations. We studied a set of 135 RILs originating from a cross between an emmer cultivar (Dic2) and a recent durum elite cultivar (Silur). Six thousand sequence baits were designed to target Dic2 vs. Silur polymorphisms discovered in a previous RNAseq study. These baits were exposed to genomic DNA of the RIL population. Eighty percent of the targeted SNPs were recovered, 65% of which were of high quality and coverage. The final high density genetic map consisted of more than 3,000 markers, whose genetic and physical mapping were consistent with those obtained with large arrays.
authors.affiliation: Montpellier SupAgro, UMR AGAP, Montpellier, France.
authors.forename: Yan
authors.initials: Y
authors.lastname: Holtz
authors.affiliation: INRA, UMR AGAP, Montpellier, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Ardisson
authors.affiliation: Montpellier SupAgro, UMR AGAP, Montpellier, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Ranwez
authors.affiliation: INRA, UMR AGAP, Montpellier, France.
authors.forename: Alban
authors.initials: A
authors.lastname: Besnard
authors.affiliation: INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, Clermont Ferrand, France.|UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, Clermont Ferrand, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Leroy
authors.affiliation: INRA, UMR AGAP, Montpellier, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Poux
authors.affiliation: INRA, UMR AGAP, Montpellier, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Roumet
authors.affiliation: INRA, UMR AGAP, Montpellier, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Viader
authors.affiliation: INRA, UMR AGAP, Montpellier, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Santoni
authors.affiliation: Montpellier SupAgro, UMR AGAP, Montpellier, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: David
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D020451:Contig Mapping; D060005:Genotyping Techniques / Q000379:methods*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D014908:Triticum / Q000235:genetics*