title: Simultaneous Stabilization and Multimerization of a Peptide α-Helix by Stapling Polymerization.
abstract: Maintaining specific conformations of peptide ligands is crucial for improving the efficacy of biological interactions. Here, a one-pot polymerization strategy for stabilizing the α-helical conformation of peptides while simultaneously constructing multimeric ligands is presented. The new method, termed stapling polymerization, uses radical polymerization between acryloylated peptide side chains and vinylic monomers. Studies with model peptides indicate that i, i+7 crosslinking is effective for the helix stabilization, whereas i, i+4 crosslinking is not. The stapling polymerization results in the formation of peptide-polyacrylamide conjugates that include ≈3-16 peptides in a single conjugate. This stapling polymerization provides a simple but powerful methodology to fabricate multimeric α-helices that can further be developed to modulate multivalent biomacromolecular interactions.
authors.affiliation: Department of Materials Science & Engineering, Yonsei University, 50 Yonsei-ro, Seoul, 03722, South Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Materials Science & Engineering, Yonsei University, 50 Yonsei-ro, Seoul, 03722, South Korea.
authors.forename: Sanghun
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Materials Science & Engineering, Yonsei University, 50 Yonsei-ro, Seoul, 03722, South Korea.
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Lim
mesh_terms: D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D058105:Polymerization; D055550:Protein Stability; D017433:Protein Structure, Secondary
chemical_list: D010455:Peptides
keywords: biopolymers; peptides; polyacrylamides; stapling polymerization; α-helices