title: Sdt97: A Point Mutation in the 5' Untranslated Region Confers Semidwarfism in Rice.
abstract: Semidwarfism is an important agronomic trait in rice breeding programs. The semidwarf mutant gene Sdt97 was previously described. However, the molecular mechanism underlying the mutant is yet to be elucidated. In this study, we identified the mutant gene by a map-based cloning method. Using a residual heterozygous line (RHL) population, Sdt97 was mapped to the long arm of chromosome 6 in the interval of nearly 60 kb between STS marker N6 and SNP marker N16 within the PAC clone P0453H04. Sequencing of the candidate genes in the target region revealed that a base transversion from G to C occurred in the 5' untranslated region of Sdt97 qRT-PCR results confirmed that the transversion induced an obvious change in the expression pattern of Sdt97 at different growth and developmental stages. Plants transgenic for Sdt97 resulted in the restoration of semidwarfism of the mutant phenotype, or displayed a greater dwarf phenotype than the mutant. Our results indicate that a point mutation in the 5' untranslated region of Sdt97 confers semidwarfism in rice. Functional analysis of Sdt97 will open a new field of study for rice semidwarfism, and also expand our knowledge of the molecular mechanism of semidwarfism in rice.
authors.affiliation: Department of Rice Breeding, Tianjin Crop Institute, Tianjin P.R., 300384 China tongjiping@sina.com goodrice@263.net.
authors.forename: Jiping
authors.initials: J
authors.lastname: Tong
authors.affiliation: Department of Rice Breeding, Tianjin Crop Institute, Tianjin P.R., 300384 China.
authors.forename: Zhengshu
authors.initials: Z
authors.lastname: Han
authors.affiliation: Department of Rice Breeding, Tianjin Crop Institute, Tianjin P.R., 300384 China.
authors.forename: Aonan
authors.initials: A
authors.lastname: Han
authors.affiliation: Department of Rice Breeding, Tianjin Crop Institute, Tianjin P.R., 300384 China tongjiping@sina.com goodrice@263.net.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Rice Breeding, Shangdong Rice Institute, Shandong Province, P.R. 250100 China.
authors.forename: Shiyong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Crop Molecular Biology, Institute of Crop Sciences of CAAS, Beijing P.R., 10081 China.
authors.forename: Binying
authors.initials: B
authors.lastname: Fu
authors.affiliation: Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province P.R., 430072 China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Rice Breeding, Tianjin Crop Institute, Tianjin P.R., 300384 China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Su
authors.affiliation: Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province P.R., 430072 China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Rice Breeding, Tianjin Crop Institute, Tianjin P.R., 300384 China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province P.R., 430072 China.
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D001947:Breeding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056726:Genetic Association Studies; D005838:Genotype; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D030821:Plants, Genetically Modified; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable
chemical_list: D020121:5' Untranslated Regions
keywords: Sdt97; complement test; map-based cloning; qRT-PCR; rice (Oryza sativa L.); semidwarfism; transversion