title: Translin and Trax differentially regulate telomere-associated transcript homeostasis.
abstract: Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.
authors.forename: Natalia
authors.initials: N
authors.lastname: Gomez-Escobar
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.
authors.forename: Nasser
authors.initials: N
authors.lastname: Almobadel
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.
authors.forename: Othman
authors.initials: O
authors.lastname: Alzahrani
authors.affiliation: Computational Biotechnology and Bioinformatics Group, Institute of Molecular Biotechnology, Graz University of Technology, Graz, Austria.|Omics Center Graz, BioTechMed Graz, Graz, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Feichtinger
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.
authors.forename: Vicente
authors.initials: V
authors.lastname: Planells-Palop
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Alshehri
authors.affiliation: Computational Biotechnology and Bioinformatics Group, Institute of Molecular Biotechnology, Graz University of Technology, Graz, Austria.|Omics Center Graz, BioTechMed Graz, Graz, Austria.
authors.forename: Gerhard G
authors.initials: GG
authors.lastname: Thallinger
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.
authors.forename: Jane A
authors.initials: JA
authors.lastname: Wakeman
authors.affiliation: North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor, Gwynedd, United Kingdom.
authors.forename: Ramsay J
authors.initials: RJ
authors.lastname: McFarlane
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000235:genetics / Q000378:metabolism*; D059505:Telomere Homeostasis; D059467:Transcriptome; D014162:Transfection
chemical_list: C481643:Ago1 protein, S pombe; D060565:Argonaute Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D012331:RNA, Fungal; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; C502801:TRAX protein, S pombe; C095563:TSN protein, human; C498305:TSNAX protein, human; C502800:tsn1 protein, S pombe
keywords: C3PO; Chromosome Section; TERRA; Translin; Trax; telomeres