title: Optimization of infrared-assisted extraction of Bletilla striata polysaccharides based on response surface methodology and their antioxidant activities.
abstract: Bletilla striata polysaccharides (BSP) have attracted extensive research interest due to their potential medical application. Herein, infrared-assisted technique is employed for the first time to extract BSP from B. striata (Thunb.) Reichb.f. based on a Box-Behnken design (BBD) and response surface methodology, with the optimum extraction parameters as follows: 75°C extraction temperature, 2.5h extraction time; and water to solid ratio (53ml/g). Based on it, 43.95±0.26% yield of crude BSP was obtained. Subsequently, crude BSP was further decolorized, deproteinized, freeze-dried, and purified by a DEAE-52 cellulose column. Furthermore, the micro-structure and a triple-helical structure of BSP were characterized. Fourier transform infrared spectra confirmed its polysaccharide characterization via typical peaks. In addition, the significant in vitro antioxidant profiles of BSP were demonstrated by superoxide anion radical-scavenging assay, hydroxyl radical scavenging assay, DPPH free radical scavenging activity and chelation of ferrous ions. Taken together, this study provide an efficient extraction technique for BSP as a promising natural antioxidant.
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST, Chengdu 611137, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST, Chengdu 611137, China.
authors.forename: Chunxue
authors.initials: C
authors.lastname: Li
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST, Chengdu 611137, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, China. Electronic address: ruizeng028@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST, Chengdu 611137, China. Electronic address: chaomeifu@126.com.
authors.forename: Chaomei
authors.initials: C
authors.lastname: Fu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism; D007259:Infrared Rays; D029595:Orchidaceae / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D017665:Hydroxyl Radical
keywords: Antioxidant activity; Bletilla striata polysaccharides; Infrared-assisted extraction; Optimization; Response surface methodology