title: Toxic effects of cisplatin cytostatic drug in mussel Mytilus galloprovincialis.
abstract: Antineoplastic drugs used in chemotherapy were detected in aquatic environment: despite the very low concentrations (ng L(-1) to ug L(-1)), due to their potent mechanism of action they could have adverse effects on non-target aquatic organisms particularly under chronic exposure. Cisplatin (CDDP) is one of the most effective anticancer drug currently in use but information on its ecotoxicological effects is very limited. In this study, Mytilus galloprovincialis was used to investigate the toxic effects related to CDDP exposure. Mussels were exposed to cisplatin (100 ng L(-1)) for 14 days: antioxidant (superoxide dismutase, catalase, total and selenium-dependent glutathione peroxidase) and phase II (glutathione-S-transferase) enzymes activities, oxidative damage (lipid peroxidation), genotoxicity (DNA damage) and neurotoxicity (acetylcholinesterase) was evaluated. Results indicate that CDDP at tested concentration induce changes in the antioxidant capacity, oxidative stress in target organs (digestive gland and gills) as well as DNA damage in mussel hemocytes and neurotoxicity representing a risk for non-target organisms.
authors.affiliation: Instituto de Ciencias Marinas de Andalucía (CSIC), Campus Rio San Pedro, 11510, Puerto Real, Cádiz, Spain; CIMA - Centro de Investigação Marinha e Ambiental, Universidade do Algarve, Campus de Gambelas, 8005-397, Faro, Portugal. Electronic address: chiara.trombini@icman.csic.es.
authors.forename: Chiara
authors.initials: C
authors.lastname: Trombini
authors.affiliation: CIMA - Centro de Investigação Marinha e Ambiental, Universidade do Algarve, Campus de Gambelas, 8005-397, Faro, Portugal. Electronic address: taina-garcia@hotmail.com.
authors.forename: Taina
authors.initials: T
authors.lastname: Garcia da Fonseca
authors.affiliation: CIMA - Centro de Investigação Marinha e Ambiental, Universidade do Algarve, Campus de Gambelas, 8005-397, Faro, Portugal. Electronic address: mdbm93@gmail.com.
authors.forename: Matilde
authors.initials: M
authors.lastname: Morais
authors.affiliation: CIMA - Centro de Investigação Marinha e Ambiental, Universidade do Algarve, Campus de Gambelas, 8005-397, Faro, Portugal. Electronic address: thiagorochabio20@gmail.com.
authors.forename: Thiago Lopes
authors.initials: TL
authors.lastname: Rocha
authors.affiliation: Instituto de Ciencias Marinas de Andalucía (CSIC), Campus Rio San Pedro, 11510, Puerto Real, Cádiz, Spain. Electronic address: julian.blasco@csic.es.
authors.forename: Julián
authors.initials: J
authors.lastname: Blasco
authors.affiliation: CIMA - Centro de Investigação Marinha e Ambiental, Universidade do Algarve, Campus de Gambelas, 8005-397, Faro, Portugal. Electronic address: mbebian@ualg.pt.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Bebianno
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D054697:Cytostatic Agents / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D049878:Mytilus / Q000502:physiology*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D054697:Cytostatic Agents; D014874:Water Pollutants, Chemical; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D002945:Cisplatin
keywords: Biomarkers; Cisplatin; Genotoxicity; Mussel; Oxidative stress