title: Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis.
abstract: Piceatannol is a polyphenolic analog of resveratrol that selectively inhibits the non-receptor tyrosine kinase-Syk. This study investigates the potential ability of piceatannol to attenuate liver fibrosis and protect hepatocytes from injury. Thioacetamide was injected in adult male mice (100 mg/kg, i.p., 3 times/week) for 8 weeks. Piceatannol (1 or 5 mg/kg per day) was administered by oral gavage during the last 4 weeks. Liver function biomarkers, tissue malondialdehyde (MDA), cytokeratin-18 (CK18), hepatocyte growth factor (HGF), and interleukin-10 (IL-10) were measured. Necroinflammation, fibrosis, expression of transforming growth factor (TGF)-β1, and α-smooth muscle actin (SMA) were scored by histopathological examination and immunohistochemistry. Obtained results showed ability of piceatannol (1 mg/kg) to restore liver function and reduce inflammation. It significantly (p < 0.001) reduced MDA, CK18, TGF-β1, and α-SMA expression, and increased HGF and IL-10. It can be concluded that piceatannol at low dose can inhibit TGF-β1 induced hepatocytes apoptosis and exerts an anti-inflammatory effect attenuating fibrosis progression.
authors.affiliation: a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Hazem
authors.initials: H
authors.lastname: Abd-Elgawad
authors.affiliation: a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Nashwa
authors.initials: N
authors.lastname: Abu-Elsaad
authors.affiliation: b Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Amr
authors.initials: A
authors.lastname: El-Karef
authors.affiliation: a Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Tarek
authors.initials: T
authors.lastname: Ibrahim
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D008297:Male; D051379:Mice; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D013853:Thioacetamide / Q000633:toxicity*
chemical_list: C553307:HGF protein, mouse; C556062:IL10 protein, mouse; D020011:Protective Agents; D013267:Stilbenes; D013853:Thioacetamide; D016753:Interleukin-10; D017228:Hepatocyte Growth Factor; C041525:3,3',4,5'-tetrahydroxystilbene
keywords: HGF; IL-10; cytokeratin-18; cytokératine-18; hepatocytes; piceatannol