title: Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin.
abstract: The hair follicle (HF) is an ideal system for studying the biology and regulation of adult stem cells (SCs). This dynamic mini organ is replenished by distinct pools of SCs, which are located in the permanent portion of the HF, a region known as the bulge. These multipotent bulge SCs were initially identified as slow cycling label retaining cells; however, their isolation has been made feasible after identification of specific cell markers, such as CD34 and keratin 15 (K15). Here, we describe a robust method for isolating bulge SCs and epidermal keratinocytes from mouse HFs utilizing fluorescence activated cell-sorting (FACS) technology. Isolated hair follicle SCs (HFSCs) can be utilized in various in vivo grafting models and are a valuable in vitro model for studying the mechanisms that govern multipotency, quiescence and activation.
authors.affiliation: Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology.
authors.forename: Despina
authors.initials: D
authors.lastname: Soteriou
authors.affiliation: Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology.
authors.forename: Lana
authors.initials: L
authors.lastname: Kostic
authors.affiliation: Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology.
authors.forename: Egor
authors.initials: E
authors.lastname: Sedov
authors.affiliation: Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology.
authors.forename: Yahav
authors.initials: Y
authors.lastname: Yosefzon
authors.affiliation: Strang Laboratory of Apoptosis and Cancer Biology, Howard Hughes Medical Institute, The Rockefeller University; steller@rockefeller.edu.
authors.forename: Hermann
authors.initials: H
authors.lastname: Steller
authors.affiliation: Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology; yfuchs@technion.ac.il.
authors.forename: Yaron
authors.initials: Y
authors.lastname: Fuchs
mesh_terms: D000818:Animals; D000078404:Epidermal Cells; D018859:Hair Follicle / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology*; D051379:Mice; D039902:Multipotent Stem Cells; D013234:Stem Cells / Q000166:cytology*