title: Genes, environment and gene expression in colon tissue: a pathway approach to determining functionality.
abstract: Genetic and environmental factors have been shown to work together to alter cancer risk. In this study we evaluate previously identified gene and lifestyle interactions in a candidate pathway that were associated with colon cancer risk to see if these interactions altered gene expression. We analyzed non-tumor RNA-seq data from 144 colon cancer patients who had genotype, recent cigarette smoking, diet, body mass index (BMI), and recent aspirin/non-steroidal anti-inflammatory use data. Using a false discovery rate of 0.1, we evaluated differential gene expression between high and low levels of lifestyle exposure and genotypes using DESeq2. Thirteen pathway genes and 17 SNPs within those genes were associated with altered expression of other genes in the pathway. BMI, NSAIDs use and dietary components of the oxidative balance score (OBS) also were associated with altered gene expression. SNPs previously identified as interacting with these lifestyle factors, altered expression of pathway genes. NSAIDs interacted with 10 genes (15 SNPs) within those genes to alter expression of 28 pathway genes; recent cigarette smoking interacted with seven genes (nine SNPs) to alter expression of 27 genes. BMI interacted with FLT1, KDR, SEPN1, TERT, TXNRD2, and VEGFA to alter expression of eight genes. Three genes (five SNPs) interacted with OBS to alter expression of 12 genes. These data provide support for previously identified lifestyle and gene interactions associated with colon cancer in that they altered expression of key pathway genes. The need to consider lifestyle factors in conjunction with genetic factors is illustrated.
authors.affiliation: Department of Internal Medicine, 383 Colorow, University of Utah School of Medicine Salt Lake, USA.
authors.forename: Martha L
authors.initials: ML
authors.lastname: Slattery
authors.affiliation: Department of Internal Medicine, 383 Colorow, University of Utah School of Medicine Salt Lake, USA.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Pellatt
authors.affiliation: Department of Internal Medicine, 383 Colorow, University of Utah School of Medicine Salt Lake, USA.
authors.forename: Roger K
authors.initials: RK
authors.lastname: Wolff
authors.affiliation: Department of Internal Medicine, 383 Colorow, University of Utah School of Medicine Salt Lake, USA.
authors.forename: Abbie
authors.initials: A
authors.lastname: Lundgreen
keywords: BMI; Gene expression; NSAIDs; colon cancer; diet; inflammation