title: Darwinolide, a New Diterpene Scaffold That Inhibits Methicillin-Resistant Staphylococcus aureus Biofilm from the Antarctic Sponge Dendrilla membranosa.
abstract: A new rearranged spongian diterpene, darwinolide, has been isolated from the Antarctic Dendroceratid sponge Dendrilla membranosa. Characterized on the basis of spectroscopic and crystallographic analysis, the central seven-membered ring is hypothesized to originate from a ring-expansion of a spongian precursor. Darwinolide displays 4-fold selectivity against the biofilm phase of methicillin-resistant Staphylococcus aureus compared to the planktonic phase and may provide a scaffold for the development of therapeutics for this difficult to treat infection.
authors.affiliation: Department of Chemistry, University of South Florida , 4202 East Fowler Avenue, CHE205, Tampa, Florida 33620, United States.|Center for Drug Discovery and Innovation, University of South Florida , 3720 Spectrum Boulevard, Suite 303, Tampa, Florida 33612, United States.
authors.forename: Jacqueline L
authors.initials: JL
authors.lastname: von Salm
authors.affiliation: Department of Chemistry, University of South Florida , 4202 East Fowler Avenue, CHE205, Tampa, Florida 33620, United States.|Center for Drug Discovery and Innovation, University of South Florida , 3720 Spectrum Boulevard, Suite 303, Tampa, Florida 33612, United States.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Witowski
authors.affiliation: Center for Drug Discovery and Innovation, University of South Florida , 3720 Spectrum Boulevard, Suite 303, Tampa, Florida 33612, United States.|Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida , 4202 East Fowler Avenue, ISA2015, Tampa, Florida 33620, United States.
authors.forename: Renee M
authors.initials: RM
authors.lastname: Fleeman
authors.affiliation: Department of Biology, University of Alabama at Birmingham , Birmingham, Alabama 35294, United States.
authors.forename: James B
authors.initials: JB
authors.lastname: McClintock
authors.affiliation: Department of Biology, University of Alabama at Birmingham , Birmingham, Alabama 35294, United States.
authors.forename: Charles D
authors.initials: CD
authors.lastname: Amsler
authors.affiliation: Center for Drug Discovery and Innovation, University of South Florida , 3720 Spectrum Boulevard, Suite 303, Tampa, Florida 33612, United States.|Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida , 4202 East Fowler Avenue, ISA2015, Tampa, Florida 33620, United States.
authors.forename: Lindsey N
authors.initials: LN
authors.lastname: Shaw
authors.affiliation: Department of Chemistry, University of South Florida , 4202 East Fowler Avenue, CHE205, Tampa, Florida 33620, United States.|Center for Drug Discovery and Innovation, University of South Florida , 3720 Spectrum Boulevard, Suite 303, Tampa, Florida 33612, United States.
authors.forename: Bill J
authors.initials: BJ
authors.lastname: Baker
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011161:Porifera / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D004224:Diterpenes