title: Biological characteristics of renal cancer cells after CTP-mediated cancer suppressor gene NPRL2 protein treatment.
abstract: Nitrogen permease regulator like-2 (NPRL2) has been proved to be a useful suppressor gene in treating many cancers containing renal cancer based on experiments. Transgenic technology which transfect exogenous NPRL2 gene into cancer cell was used in these experiments. However, this technology has defects, such as gene mutation and loss. Cytoplasmic transduction peptide (CTP) can be used to avoid these defects because it can directly mediate proteins to penetrate cell membrane and specifically locate in cytoplasm. In this article, CTP was used to directly mediate NPRL2 protein into the renal cancer cell line 786-O, then cell proliferation was detected by the CCK-8 method, cell cycle and apoptosis were detected by flow cytometry, cell invasion and migration ability were detected by the Transwell assay. Bcl-xl, Cyt-c and caspase-3 were detected by real-time fluorescent quantitative PCR and Western blot for the analysis of the related mechanism. The result showed that CTP successfully mediated NPRL2 protein into renal cancer cells and the growth of cells was significantly inhibited. The mechanism may be NPRL2 down-regulating the expression of Bcl-xl which can up-regulate Cyt-c and further activate caspase-3, and then a cascade reaction is caused for cell apoptosis on the classic mitochondrial apoptosis pathway.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zeng
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Shi
authors.forename: Zheng-Yong
authors.initials: ZY
authors.lastname: Yuan
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
mesh_terms: D002292:Carcinoma, Renal Cell / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D045304:Cytochromes c / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism*; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: C492816:NPRL2 protein, human; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; D051020:bcl-X Protein; D045304:Cytochromes c; D053148:Caspase 3