title: Dibutyltin-induced alterations of interleukin 1beta secretion from human immune cells.
abstract: Dibutyltin (DBT) is used to stabilize polyvinyl chloride plastics (including pipes that distribute drinking water) and as a de-worming agent in poultry. DBT is found in human blood, and DBT exposures alter the secretion of tumor necrosis factor alpha and interferon gamma from lymphocytes. Interleukin (IL)-1β is a proinflammatory cytokine that regulates cellular growth, tissue restoration and immune response regulation. IL-1β plays a role in increasing invasiveness of certain tumors. This study reveals that exposures to DBT (24 h, 48 h and 6 days) modify the secretion of IL-1β from increasingly reconstituted preparations of human immune cells (highly enriched human natural killer cells, monocyte-depleted [MD] peripheral blood mononuclear cells [PBMCs], PBMCs, granulocytes and a preparation combining both PBMCs and granulocytes). DBT altered IL-1β secretion from all cell preparations. Higher concentrations of DBT (5 and 2.5 μm) decreased the secretion of IL-1β, while lower concentrations of DBT (0.1 and 0.05 μm) increased the secretion of IL-1β. Selected signaling pathways were examined in MD-PBMCs to determine if they play a role in DBT-induced elevations of IL-1β secretion. Pathways examined were IL-1β converting enzyme (caspase 1), mitogen-activated protein kinases and nuclear factor kappa B. Caspase 1 and mitogen-activated protein kinase pathways appear to be utilized by DBT in increasing IL-1β secretion. These results indicate that DBT alters IL-1β secretion from human immune cells in an ex. vivo system utilizing several IL-1β regulating signaling pathways. Thus, DBT may have the potential to alter IL-1β secretion in an in vivo system. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Biological Sciences, Tennessee State University, Nashville, TN, USA.
authors.forename: Shyretha
authors.initials: S
authors.lastname: Brown
authors.affiliation: Department of Chemistry, Tennessee State University, Nashville, TN, USA.
authors.forename: Shahin
authors.initials: S
authors.lastname: Tehrani
authors.affiliation: Department of Chemistry, Tennessee State University, Nashville, TN, USA.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Whalen
mesh_terms: D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D004785:Environmental Pollutants / Q000633:toxicity*; D006098:Granulocytes / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D009947:Organotin Compounds / Q000633:toxicity*
chemical_list: D004785:Environmental Pollutants; D053583:Interleukin-1beta; D009947:Organotin Compounds; C056189:di-n-butyltin
keywords: MD-PBMCs; NK cells; PBMCs; dibutyltin; granulocytes; interleukin 1 beta