title: Thiol-ene Clickable Poly(glycidol) Hydrogels for Biofabrication.
abstract: In this study we introduce linear poly(glycidol) (PG), a structural analog of poly(ethylene glycol) bearing side chains at each repeating unit, as polymer basis for bioink development. We prepare allyl- and thiol-functional linear PG that can rapidly be polymerized to a three-dimensionally cross-linked hydrogel network via UV mediated thiol-ene click reaction. Influence of polymer concentration and UV irradiation on mechanical properties and swelling behavior was examined. Thiol-functional PG was synthesized in two structural variations, one containing ester groups that are susceptible to hydrolytic cleavage, and the other one ester-free and stable against hydrolysis. This allowed the preparation of degradable and non-degradable hydrogels. Cytocompatibility of the hydrogel was demonstrated by encapsulation of human bone marrow-derived mesenchymal stem cells (hBMSCs). Rheological properties of the hydrogels were adjusted for dispense plotting by addition of high molecular weight hyaluronic acid. The optimized formulation enabled highly reproducible plotting of constructs composed of 20 layers with an overall height of 3.90 mm.
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Stichler
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Jungst
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.
authors.forename: Martha
authors.initials: M
authors.lastname: Schamel
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.
authors.forename: Ilona
authors.initials: I
authors.lastname: Zilkowski
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kuhlmann
authors.affiliation: Department of Trauma, Hand, Plastic and Reconstructive Surgery, University of Würzburg, Oberdürrbacher Str. 6, 97080, Würzburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Böck
authors.affiliation: Department of Trauma, Hand, Plastic and Reconstructive Surgery, University of Würzburg, Oberdürrbacher Str. 6, 97080, Würzburg, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Blunk
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Teßmar
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany. juergen.groll@fmz.uni-wuerzburg.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Groll
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D057930:Click Chemistry; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011409:Propylene Glycols / Q000737:chemistry / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D020100:Hydrogels; D011409:Propylene Glycols; C502907:polyglycidol
keywords: Bioink; Bioprinting; Dispense plotting; Hyaluronic acid; Poly(glycidol)