title: Chemical constituents from Inonotus obliquus and their antitumor activities.
abstract: Four new lanostane-type triterpenes (inonotusanes D-G, 1-4), including a 24,25,26,27-tetranorlanostane, together with 11 known compounds (5-15), including 7 lanostane derivatives, 2 steroids and 2 aromatic compounds, were isolated from the sclerotia of Inonotus obliquus. Their structures were elucidated by 1D and 2D NMR spectroscopy and HRMS. To our knowledge, 1 is the first 24,25,26,27-tetranorlanostane-type triterpenoid from fungus, and this is the first time that 31-member lanostane-type triterpenes, 5 and 6, have been isolated from the sclerotia of I. obliquus instead of from its submerged culture. 7 and 8 are also new isolates of this genus. Compounds 1, 8, 12 and 13 exhibited strong cytotoxicity against the 4T1 (mouse breast cancer) cell line, with IC50 9.40, 7.79, 9.06 and 9.31 μM, respectively. 8, 12 and 13 also exhibited strong cytotoxicity against the the MCF-7 (human breast cancer) cell line, with IC50 8.35-9.01 μM.
authors.affiliation: Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China.|Institute of Pharmacy, Henan University, Jinming Street, Kaifeng, 475004, China.
authors.forename: Fenqin
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China.|School of Chinese Materia Medica and Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin, 300193, China.
authors.forename: Guiyang
authors.initials: G
authors.lastname: Xia
authors.affiliation: Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Pharmacy, Henan University, Jinming Street, Kaifeng, 475004, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Institute of Pharmacy, Henan University, Jinming Street, Kaifeng, 475004, China.
authors.forename: Zhanfang
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, China. fengqiu20070118@163.com.|School of Chinese Materia Medica and Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin, 300193, China. fengqiu20070118@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Institute of Pharmacy, Henan University, Jinming Street, Kaifeng, 475004, China. hanghang1998@126.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Han
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D025282:Mycelium; D008517:Phytotherapy; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D001688:Biological Products; D014315:Triterpenes; C000615792:inonotusane D
keywords: Antitumor; Inonotus obliquus; Inonotusane D; Inonotusane E; Inonotusane F; Inonotusane G; Triterpenoids