title: Role of mesenchymal stem cells versus angiotensin converting enzyme inhibitor in kidney repair.
abstract: OBJECTIVE
  The current study sought to clarify the role of bone marrow derived mesenchymal stem cells (BM-MSCs) and adipose tissue derived mesenchymal stem cells (AD-MSCs) in repressing nephropathy in the experimental model. Moreover, the aim of this work was extended to compare between stem cells role and angiotensin converting enzyme inhibitor in kidney repair.
  
  
  METHODS
  Isolation and preparation of MSCs culture, flow cytometry using CD34, CD44 and CD105 cell surface markers, biochemical analyses for determination of serum creatinine, urea, transforming growth factor β (TGF-β), cystatin C (CYS-C) and urinary N-Acetyl-ß-D-Glucosaminidase (UNAG), and histopathological investigation of kidney tissue sections were performed.
  
  
  RESULTS
  The results of the present study revealed that single intravenous infusion of MSCs either derived from bone marrow or adipose tissue was able to enhance renal reparative processes through significantly decreased serum creatinine, urea, TGF-β and CYS-C levels as well as UNAG level and significantly increase glomerular filtration rate. Additionally, the histopathological investigations of kidney tissues showed that MSCs have significant regenerative effects as evidenced by the decrease in focal inflammatory cells infiltration, focal interstitial nephritis and congested glomeruli as well as degenerated tubules.
  
  
  CONCLUSIONS
  The current data provided distinct evidence about the favourable impact of AD-MSCs and BM-MSCs in attenuation of cyclosporine-induced nephropathy in rats through their ability to promote functional and structural kidney repair via transdifferentiation.
authors.affiliation: Hormones Department, National Research Centre, Dokki, Giza, Egypt.
authors.forename: Hanaa H
authors.initials: HH
authors.lastname: Ahmed
authors.affiliation: Chemistry Department, Faculty of Science, Damietta University, Damietta, Egypt.
authors.forename: Elshahat A
authors.initials: EA
authors.lastname: Toson
authors.affiliation: Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt.
authors.forename: Hatem A
authors.initials: HA
authors.lastname: El-Mezayen
authors.affiliation: Department of Medical Biochemistry, Unit of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Laila A
authors.initials: LA
authors.lastname: Rashed
authors.affiliation: Chemistry Department, Faculty of Science, Damietta University, Damietta, Egypt.
authors.forename: Eslam S
authors.initials: ES
authors.lastname: Elsherbiny
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001854:Bone Marrow Cells / Q000502:physiology*; D019070:Cell Lineage; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D016572:Cyclosporine; D004195:Disease Models, Animal; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology / Q000628:therapy*; D017706:Lisinopril / Q000494:pharmacology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010012:Osteogenesis; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects*; D051876:Sex-Determining Region Y Protein / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D015415:Biomarkers; D051876:Sex-Determining Region Y Protein; C497747:Sry protein, rat; D016572:Cyclosporine; D017706:Lisinopril
keywords: N-Acetyl-ß-D-glucosaminidase; angiotensin converting enzyme inhibitors; cyclosporine; mesenchymal stem cells; nephrotoxicity; transforming growth factor β