title: Comparison of nerve regenerative efficacy between decellularized nerve graft and nonwoven chitosan conduit.
abstract: BACKGROUND
  Recently decellularized nerves with various methods are reported as highly functional nerve grafts for the treatment of nerve defects.
  
  
  OBJECTIVE
  To evaluate the efficacy of decellularized allogeneic nerve, compared with oriented chitosan mesh tube, and an autologous nerve.
  
  
  METHODS
  Sciatic nerves harvested from Sprague-Dawley (SD) rats were decellularized in combination with Sodium dodecyl sulfate and Triton X-100. A graft into the sciatic nerve in Wistar rats was performed with the decellularized SD rat sciatic nerves or oriented chitosan nonwoven nanofiber mesh tubes (15 mm in length, N=5 in each group). A portion of sciatic nerve of Wistar rat was cut, reversed and re-sutured in-situ as a control. Nerve functional and histological evaluations were performed 25 weeks postoperatively.
  
  
  RESULTS
  It was revealed that functional, electrophysiological and histological recoveries in the decellularized nerve group match those in the autograft group. Recovery of sensory function and nerve maturation in the decellularized nerve group were superior to those in the chitosan mesh tube group.
  
  
  CONCLUSIONS
  Nerve regeneration in the decellularized nerves could match that in the autografts and is somehow superior to artificial chitosan mesh tube. Detergents wash of SDS and Triton X-100 could obtain highly functional nerve grafts from allografts.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kusaba
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Terada-Nakaishi
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.|Department of Orthopaedic Surgery, Sakurakai Hospital, 2-13-1 Senju-Sakuragi, Adachi-ku, Tokyo 120-0045, Japan. E-mail: itoso.gene@gmail.com.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Itoh
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nozaki
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Nagai
authors.affiliation: Research Center for Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo 113-8510, Japan.
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ichinose
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takakuda
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D008297:Male; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration; D017830:Octoxynol / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology* / Q000601:surgery / Q000637:transplantation*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol; D048271:Chitosan
keywords: Decellularized nerve; chitosan mesh tube; peripheral nerve regeneration