title: Sodium butyrate-induced apoptosis and ultrastructural changes in MCF-7 breast cancer cells.
abstract: This study investigated the effects of sodium butyrate (NaB) on Michigan Cancer Foundation-7 (MCF-7) breast cancer cells and analyzed the relevant mechanism. Here, we demonstrated that a certain concentration of NaB effectively induced MCF-7 cell apoptosis. Cell counting kit-8 (CCK-8) assay was used to detect cell viability and the apoptosis rate. Western blotting was used to detect changes in the Bcl-2 expression level. We observed cell shape changes with microscopy. Immunofluorescence revealed some apoptotic nuclei. Electron microscopy revealed thick nucleoli, chromatin margination, reduced mitochondria, and dramatic vacuoles. Collectively, our findings elucidated the morphological mechanism by which NaB changed the ultrastructure of MCF-7 cells.
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences , Wuhan University , Wuhan , Hubei , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences , Wuhan University , Wuhan , Hubei , China.
authors.forename: Peng-Chao
authors.initials: PC
authors.lastname: Hu
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences , Wuhan University , Wuhan , Hubei , China.
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Ma
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences , Wuhan University , Wuhan , Hubei , China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences , Wuhan University , Wuhan , Hubei , China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Gao
authors.affiliation: b Department of Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center , Wuhan University , Wuhan , Hubei , China.
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Zhang
authors.affiliation: a Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences , Wuhan University , Wuhan , Hubei , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wei
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000648:ultrastructure*; D020148:Butyric Acid / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005455:Fluorescent Antibody Technique; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission
chemical_list: D000970:Antineoplastic Agents; D056572:Histone Deacetylase Inhibitors; D020148:Butyric Acid
keywords: MCF-7; sodium butyrate; ultrastructural changes