title: Study on anti-inflammatory efficacy and correlative ingredients with pharmacodynamics detected in acute inflammation rat model serum from Caulis Lonicerae japonicae.
abstract: BACKGROUND
  Caulis Lonicerae japonicae (CLJ) is often used for the treatment of inflammation such as acute fever, headache, respiratory infection and epidemic diseases. Nevertheless, domestic and foreign researches simply fail to focus on reports of CLJ, especially its anti-inflammatory effects and correlative components.
  
  
  OBJECTIVE
  In this study, we investigated anti-inflammatory effects and serum components of Caulis Lonicerae japonicae (CLJ) in models of acute inflammation and correlative analysis between anti-inflammatory effects and serum components of CLJ in rat serum to analyze the changes in the relative contents of components in serum with time and in the corresponding values to characterize active fractions of CLJ and identify the major active components of CLJ in rats.
  
  
  METHODS
  Active fractions of CLJ were screened using xylene-induced ear oedema mice model. Anti-inflammatory effects were evaluated using carrageenan-induced paw oedema rat model, and then correlative components in rat serum were demonstrated with HPLC-QTOF/MS/MS method explored.
  
  
  METHODS
  HPLC-QTOF/MS/MS was developed to analyze the components absorbed in rat serum after oral administration of CLJ.
  
  
  RESULTS
  Ethyl acetate extracts (ECLJ) and n-butanol extracts (BCLJ) of CLJ were preliminarily screened as active fractions of CLJ (EBCLJ) using xylene-induced ear oedema mice model, and effectively inhibited edema and values of interleukin-1 (IL-l), nuclear factor-kappaB (NF-kB), tumor necrosis factor-α (TNF-α) activities and prostaglandinE2 (PGE2) productions using carrageenan-induced paw oedema rat model. 33 peaks were found in total ion current chromatograms of EBCLJ, and 27 components were observed in EBCLJ-treated rat serum, only 11 of which were correlated with anti-inflammatory effects.
  
  
  CONCLUSIONS
  The present study contributes to the study on the pharmacodynamic basis of CLJ and provides potent evidence for developing CLJ as a safe and promising natural drug in inflammation treatment.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Tang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China. Electronic address: njxlyl2002@163.com.
authors.forename: Lian
authors.initials: L
authors.lastname: Yin
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China.
authors.forename: Yida
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China.
authors.forename: Fangli
authors.initials: F
authors.lastname: Zhao
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Cui
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China.
authors.forename: Le
authors.initials: L
authors.lastname: Shi
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 210023 Nanjing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002681:China; D004195:Disease Models, Animal; D007249:Inflammation / Q000188:drug therapy*; D031239:Lonicera / Q000737:chemistry*; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts
keywords: Active fractions; Anti-inflammatory effect; Caulis Lonicerae japonicae; Correlation analysis between anti-inflammatory effects and serum components; Serum components