title: Periplocin Extracted from Cortex Periplocae Induced Apoptosis of Gastric Cancer Cells via the ERK1/2-EGR1 Pathway.
abstract: OBJECTIVE
  Periplocin is extracted from the traditional herbal medicine cortex periplocae, which has been reported to suppress the growth of cancer cells. However, little is known about its effect on gastric cancer cells.
  
  
  METHODS
  Gastric cancer cells were treated with periplocin, and cell viability was assessed using MTS assay. Flow cytometry and TUNEL staining were performed to evaluate apoptosis, and protein expression was examined by western blotting. Microarray analysis was used to screen for changes in related genes.
  
  
  RESULTS
  We found that periplocin had an inhibitory effect on gastric cancer cell viability in a dose-dependent manner. Periplocin inhibited cell viability via the ERK1/2-EGR1 pathway to induce apoptosis. Periplocin also inhibited the growth of tumor xenografts and induced apoptosis in vivo.
  
  
  CONCLUSIONS
  Our results show that periplocin inhibits the proliferation of gastric cancer cells and induces apoptosis in vitro and in vivo, indicating its potential to be used as an antitumor drug.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Lian-Mei
authors.initials: LM
authors.lastname: Zhao
authors.forename: Su-Li
authors.initials: SL
authors.lastname: Dai
authors.forename: Wen-Xuan
authors.initials: WX
authors.lastname: Cui
authors.forename: Hui-Lai
authors.initials: HL
authors.lastname: Lv
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Bao-En
authors.initials: BE
authors.lastname: Shan
mesh_terms: D000818:Animals; D029062:Apocynaceae / Q000737:chemistry* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology; D014183:Transplantation, Heterologous
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D010936:Plant Extracts; D012503:Saponins; C502381:periplocin; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3