title: Purification, structural characterization and in vitro antioxidant activity of a novel polysaccharide from Boshuzhi.
abstract: A novel polysaccharide, designated GCPB-1b, was obtained from the alkaline extract of the submerged fermentation culturing mycelium powder of Boshuzhi, Ganoderma capense. This polysaccharide was purified by ion-exchange and gel-permeation column chromatography and is a glucan (MW 2847Da). GCPB-1b has a specific optical rotation of [α]D(25)=+154° (c 1.0, H2O). Based on monosaccharide analysis, partial acid hydrolysis, periodic acid oxidation-Smith degradation, methylation analysis, Fourier transform-infrared spectroscopy (FT-IR), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy, the backbone of GCPB-1b was composed of (1→4)-linked-α-d-glucose residues and (1→4,6)-linked-α-d-glucose residues. Furthermore, GCPB-1b had 1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging ability (EC50 3.23μM) according to antioxidant activity tests which was greater than other antioxidants. These data suggest that GCPB-1b holds promise as an anti-aging functional food.
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, PR China; Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, PR China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Fansheng
authors.initials: F
authors.lastname: Kong
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Naisheng
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, PR China; Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, PR China. Electronic address: ycybridge@163.com.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yan
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Huachong
authors.initials: H
authors.lastname: Lv
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D032902:Ganoderma / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: 1-Diphenyl-2-picryl-hydrazyl (PubChem CID: 74358); Ascorbic acid (PubChem CID: 54670067); Boshuzhi; Characterization; Chloroform (PubChem CID: 6212); Dimethyl sulfoxide (PubChem CID: 679); Ganoderma capense; Hydroxylamine hydrochloride (PubChem CID: 443297); Methanol (PubChem CID: 887); Polysaccharide; Pyridine (PubChem CID: 1049); Radical-scavenging activity; Sodium chloride (PubChem CID: 5234); Sodium hydroxide (PubChem CID: 14798); Trifluoroacetic acid (PubChem CID: 6422)