title: Anesthetic isoflurane attenuates activated microglial cytokine-induced VSC4.1 motoneuronal apoptosis.
abstract: Isoflurane (ISO) exhibits neuroprotective effects against inflammation and apoptosis. However, the role of ISO in motoneuronal apoptosis induced by activated microglia remains poorly studied. We investigated the protective effects of ISO on the apoptosis of ventral spinal cord 4.1 (VSC4.1) motoneurons induced by lipopolysaccharide (LPS)-activated BV-2 microglia. Results indicated that ISO inhibited NF-κB activation and pro-inflammatory cytokine release in LPS-treated BV-2 microglia. Conditioned medium (CM) from activated BV-2 cells treated by ISO directly prevented VSC4.1 motoneurons from LPS-CM-induced neuronal apoptosis, as determined by the following: reductions in caspase-8, caspase-9, and caspase-3 activities; downregulation of pro-apoptotic procaspase-8, cleaved (cl)-caspase-8, procaspase-9, cl-caspase-9, caspase-3, cl-caspase-3, Bid, Bax, and cytochrome c expression; and upregulation of anti-apoptotic Bcl-2 expression in LPS-CM-cultured VSC4.1 motoneurons. Findings demonstrated that ISO inhibits BV-2 microglia activation and alleviates VSC4.1 motoneuronal apoptosis induced by microglial activation. These effects suggest that ISO can be used as an alternative agent for reducing neuronal apoptosis.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Xinxiang, Henan, People's Republic of China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Xinxiang, Henan, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Xinxiang, Henan, People's Republic of China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Xinxiang, Henan, People's Republic of China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Xinxiang, Henan, People's Republic of China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Xinxiang, Henan, People's Republic of China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Xinxiang, Henan, People's Republic of China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Yue
keywords: BV-2 microglia; Isoflurane; LPS; NF-κB; VSC4.1 motoneuron; apoptosis