title: The Toolbox for Conditional Zebrafish Cancer Models.
abstract: Here we describe the conditional zebrafish cancer toolbox, which allows for fine control of the expression of oncogenes or downregulation of tumor suppressors at the spatial and temporal level. Methods such as the Gal4/UAS or the Cre/lox systems paved the way to the development of elegant tumor models, which are now being used to study cancer cell biology, clonal evolution, identification of cancer stem cells and anti-cancer drug screening. Combination of these tools, as well as novel developments such as the promising genome editing system through CRISPR/Cas9 and clever application of light reactive proteins will enable the development of even more sophisticated zebrafish cancer models. Here, we introduce this growing toolbox of conditional transgenic approaches, discuss its current application in zebrafish cancer models and provide an outlook on future perspectives.
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
authors.forename: Marie
authors.initials: M
authors.lastname: Mayrhofer
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany. maria.mione@kit.edu.
authors.forename: Marina
authors.initials: M
authors.lastname: Mione
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004195:Disease Models, Animal; D006728:Hormones / Q000502:physiology; D009369:Neoplasms / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D062308:Optogenetics; D015027:Zebrafish
chemical_list: D006728:Hormones
keywords: Cancer models; Cre/lox; ER/tamoxifen; Gal4/UAS; Heat shock; Inducible systems; Tet-On; Transgenic methods; Zebrafish