title: The preprotein translocase YidC controls respiratory metabolism in Mycobacterium tuberculosis.
abstract: The YidC-Oxa1-Alb3 preprotein translocases play a vital role in membrane insertion of proteins in eukaryotes and bacteria. In a recent study we observed that Rv3921c, which encodes putative YidC translocase in Mycobacterium tuberculosis (Mtb), is essential for in vitro growth of bacteria. However, the exact function of this particular protein remains to identify in mycobacterial pathogens. By performing a systematic study here we show that YidC of Mtb is an envelope protein, which is required for production of ATP and maintenance of cellular redox balance. Drastic effects of depletion of Rv3921c on the expression of hypoxic genes, ATP synthases, and many proteins of central metabolic and respiratory pathways shed a significant light on the function of YidC towards controlling respiratory metabolism in Mtb. Association of YidC with proteins such as succinate dehydrogenases and ubiquinol-cytochrome C reductase further confirms its role in respiration. Finally we demonstrate that YidC is required for the intracellular survival of Mtb in human macrophages.
authors.affiliation: Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121001 India.|Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Thakur
authors.affiliation: Regional Center for Biotechnology, NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad- 121001 India.
authors.forename: Nagavara Prasad
authors.initials: NP
authors.lastname: Gantasala
authors.affiliation: Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121001 India.|Symbiosis School of Biomedical Sciences, Symbiosis International University, Lavale, Pune- 412115 (Maharashtra) India.
authors.forename: Eira
authors.initials: E
authors.lastname: Choudhary
authors.affiliation: Regional Center for Biotechnology, NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad- 121001 India.
authors.forename: Nirpendra
authors.initials: N
authors.lastname: Singh
authors.affiliation: Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi-110062, India.
authors.forename: Malik Zainul
authors.initials: MZ
authors.lastname: Abdin
authors.affiliation: Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad 121001 India.
authors.forename: Nisheeth
authors.initials: N
authors.lastname: Agarwal
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D050296:Microbial Viability; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D010084:Oxidation-Reduction; D000069816:SEC Translocation Channels / Q000378:metabolism*; D000081416:SecA Proteins
chemical_list: D001426:Bacterial Proteins; D045222:Electron Transport Chain Complex Proteins; D000069816:SEC Translocation Channels; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins