title: Elevated CETP Lipid Transfer Activity is Associated with the Risk of Venous Thromboembolism.
abstract: OBJECTIVE
  Cholesteryl ester transfer protein (CETP) is an important lipid transfer factor in plasma that enhances prothrombinase activity in purified systems. This study was conducted to test the association of plasma CETP activity with venous thrombosis (VTE) and to address the procoagulant mechanism of CETP activity in prothrombinase assays.
  
  
  METHODS
  We measured CETP lipid transfer activity in plasmas of 49 male VTE patients and in plasmas of matched controls. CETP procoagulant activity was tested in purified prothrombinase systems.
  
  
  RESULTS
  CETP lipid transfer activity levels were significantly higher in VTE patients than in controls (p=0.0008). A subset of patients carrying the CETP mutations Ala373Pro and Arg451Gln, which were also linked to the VTE risk, showed significantly higher plasma CETP activity than the noncarriers. The plasma CETP activity negatively correlated with APTT, suggesting that the CETP activity is associated with plasma coagulability. Recombinant (r) CETP bound to both factor Xa (Kd=15 nM) and Gla-domainless factor Xa (Kd=59 nM), whereas rCETP enhanced prothrombin activation by factor Xa, but not by Gla-domainless factor Xa. rCETP also required factor Va for enhancement of prothrombinase activity. When we addressed the effects of mutations in CETP on prothrombinase activity, Gln451-rCETP was found to have five-fold higher thrombin generation activity than wt-rCETP or Pro373-rCETP.
  
  
  CONCLUSIONS
  Elevated CETP lipid transfer activity in plasma was associated with the risk of VTE. Gln451-CETP, which is linked to VTE, has much higher procoagulant activity than wt-CETP. CETP might act as a physiologic procoagulant by mechanisms that involve its direct binding to factor Xa.
authors.affiliation: Department of Molecular and Experimental Medicine, The Scripps Research Institute.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Deguchi
authors.forename: Yajnavalka
authors.initials: Y
authors.lastname: Banerjee
authors.forename: Darlene J
authors.initials: DJ
authors.lastname: Elias
authors.forename: John H
authors.initials: JH
authors.lastname: Griffin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D053480:Cholesterol Ester Transfer Proteins / Q000378:metabolism*; D018592:Cross-Over Studies; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D018570:Risk Assessment; D054556:Venous Thromboembolism / Q000175:diagnosis / Q000201:enzymology / Q000209:etiology*; D055815:Young Adult
chemical_list: C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D008055:Lipids