title: Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using ante-mortem techniques.
abstract: OBJECTIVE
  The aim of this study was to investigate the prevalence of bovine tuberculosis (TB) and detection of Mycobacterium bovis in cattle from an organized dairy farm.
  
  
  METHODS
  A total of 121 animals (93 females and 28 males) of 1 year and above were studied for the prevalence of bovine TB using single intradermal comparative cervical tuberculin (SICCT) test, bovine gamma-interferon (γ-IFN) enzyme immunoassay, and polymerase chain reactions (PCRs).
  
  
  RESULTS
  Out of total 121 animals, 17 (14.04%) animals were positive reactors to SICCT test while only one (0.82%) animal for γ-IFN assay. By PCR, Mycobacterium TB complex was detected in 19 (15.70%) animals out of which 4 (3.30%) animal were also positive for M. bovis.
  
  
  CONCLUSIONS
  Diagnosis of bovine TB can be done in early stage in live animals with multiple approaches like skin test followed by a molecular technique like PCR which showed promising results.
authors.affiliation: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India.
authors.forename: Gursimran
authors.initials: G
authors.lastname: Filia
authors.affiliation: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India.
authors.forename: Geeta Devi
authors.initials: GD
authors.lastname: Leishangthem
authors.affiliation: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Mahajan
authors.affiliation: Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana, Punjab, India.
authors.forename: Amarjit
authors.initials: A
authors.lastname: Singh
keywords: Mycobacterium bovis; Mycobacterium tuberculosis complex; bovine gamma interferon assay; bovine tuberculosis; polymerase chain reaction; single intradermal comparative cervical tuberculin test