title: Label-free bimodal waveguide immunosensor for rapid diagnosis of bacterial infections in cirrhotic patients.
abstract: Spontaneous bacterial peritonitis is an acute bacterial infection of ascitic fluid; it has a high incidence in cirrhotic patients and it is associated with high mortality. In such a situation, early diagnosis and treatment is crucial for the survival of the patient. However, bacterial analysis in ascitic fluid is currently based on culture methods, which are time-consuming and laborious. We report here the application of a photonic interferometer biosensor based on a bimodal waveguide (BiMW) for the rapid and label-free detection of bacteria directly in ascitic fluid. The device consists of a straight waveguide in which two modes of the same polarization interfere while interacting with the external medium through their evanescent fields. A bimolecular event occurring on the sensor area of the device (e.g. capturing bacteria) will differently affect each light mode, inducing a variation in the phase of the light exiting at the output of the waveguide. In this work, we demonstrate the quantitative detection of Bacillus cereus in buffer medium and Escherichia coli in undiluted ascitic fluid from cirrhotic patients. In the case of Bacillus cereus detection, the device was able to specifically detect bacteria at relevant concentrations in 12.5min and in the case of Escherichia coli detection, the analysis time was 25min. Extrapolation of the data demonstrated that the detection limits of the biosensor could reach few bacteria per milliliter. Based on the results obtained, we consider that the BiMW biosensor is positioned as a promising new clinical tool for user-friendly, cost-effective and real-time microbiological analysis.
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group. Catalan Institute of Nanoscience and Nanotechnology (ICN2). CSIC and CIBER-BBN, Campus UAB, Ed-ICN2, 08193 Barcelona, Bellaterra, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Maldonado
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group. Catalan Institute of Nanoscience and Nanotechnology (ICN2). CSIC and CIBER-BBN, Campus UAB, Ed-ICN2, 08193 Barcelona, Bellaterra, Spain.
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: González-Guerrero
authors.affiliation: Microelectronic Institute of Barcelona (IMB-CNM), CSIC, Campus UAB, 08193 Barcelona, Bellaterra, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Domínguez
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group. Catalan Institute of Nanoscience and Nanotechnology (ICN2). CSIC and CIBER-BBN, Campus UAB, Ed-ICN2, 08193 Barcelona, Bellaterra, Spain. Electronic address: laura.lechuga@icn2.cat.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Lechuga
mesh_terms: D001202:Ascitic Fluid / Q000382:microbiology; D001409:Bacillus cereus / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000150:complications* / Q000175:diagnosis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000150:complications / Q000175:diagnosis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D007368:Interferometry / Q000295:instrumentation*; D057230:Limit of Detection; D008099:Liver / Q000382:microbiology; D008103:Liver Cirrhosis / Q000150:complications* / Q000382:microbiology*
keywords: Ascitic fluid; Bacillus Cereus; Bimodal waveguide biosensor; Escherichia coli; Label-free immunoassay