title: Global transcriptional regulation by H-NS and its biological influence on the virulence of Enterohemorrhagic Escherichia coli.
abstract: As a global transcriptional regulator, H-NS, the histone-like nucleoid-associated DNA-binding and bridging protein, plays a wide range of biological roles in bacteria. In order to determine the role of H-NS in regulating gene transcription and further find out the biological significance of this protein in Enterohemorrhagic Escherichia coli (EHEC), we conducted transcriptome analysis of hns mutant by RNA sequencing. A total of 983 genes were identified to be regulated by H-NS in EHEC. 213 and 770 genes were down-regulated and up-regulated in the deletion mutant of hns, respectively. Interestingly, 34 of 97 genes on virulence plasmid pO157 were down-regulated by H-NS. Although the deletion mutant of hns showed a decreased survival rate in macrophage compared with the wild type strain, it exhibited the higher ability to colonize mice gut and became more virulent to BALB/c mice. The BALB/c mice infected with the deletion mutant of hns showed a lower survival rate, and a higher bacterial burden in the gut, compared with those infected with wild type strain, especially when the gut microbiota was not disturbed by antibiotic administration. These findings suggest that H-NS plays an important role in virulence of EHEC by interacting with host gut microbiota.
authors.affiliation: Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Baoshan
authors.initials: B
authors.lastname: Wan
authors.affiliation: Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tao
authors.affiliation: Shanghai East Hospital, Shanghai, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Zhou
authors.affiliation: Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: yfyao@sjtu.edu.cn.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Yao
authors.affiliation: Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: nijinjing45@163.com.
authors.forename: Jinjing
authors.initials: J
authors.lastname: Ni
mesh_terms: D000818:Animals; D000898:Antibiosis; D058491:Bacterial Load; D001483:Base Sequence; D004195:Disease Models, Animal; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005260:Female; D036781:Fimbriae Proteins / Q000172:deficiency / Q000235:genetics*; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D054884:Host-Pathogen Interactions; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion; D016019:Survival Analysis; D059467:Transcriptome; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins; C529458:FimG protein, E coli; D036781:Fimbriae Proteins
keywords: Enterohemorrhagic Escherichia coli; H-NS; Virulence