title: Delipidation-based solid-phase extraction pretreatment technique for plasma broad-coverage metabolomic profiling to reveal the potential pathogenesis of yeast-induced fever in rats.
abstract: During the process of metabolomics profiling by using ultra high performance liquid chromatography coupled with time-of flight mass spectrometry, blood sample pretreatment is a crucial step for biomarker discovery. Herein, in order to prevent the potential loss of metabolites and ion suppression phenomena caused by the proteins and phospholipids contained in blood fluids, a delipidation-based solid-phase extraction pretreatment technique for plasma broad-coverage metabolomic profiling was performed. This technique can be summarized as a single extraction, a single elution of solid-phase extraction plate, followed by four times measuring with electrospray ionization in positive and negative ion mode, respectively. This approach significantly increased the number of features detected in plasma, and 1572 features in positive mode and 1352 features in negative mode were detected, respectively. Besides, the stability and repeatability of the approach were greatly improved. For these advantages, the approach was employed to elucidate the potential pathogenesis of yeast-induced fever in rats. The biomarkers associated with the pathogenesis of fever were shown to be related to amino acids metabolism and lipid metabolism. The delipidation-based solid-phase extraction pretreatment approach can provide a useful tool to reveal the pathological mechanisms of such systemic pathological process.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Guo
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Gao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Lu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Fu
authors.affiliation: Waters Technologies (Shanghai) Ltd, Shanghai, P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Bai
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D005334:Fever / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000097:blood* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000472:pathogenicity; D052616:Solid Phase Extraction
chemical_list: D010743:Phospholipids; D011506:Proteins
keywords: Biomarkers; Metabolomics; Solid-phase extraction; Ultra high performance liquid chromatography; Yeast-induced fever