title: Typing and surface charges of the variable loop regions of PorB from Neisseria meningitidis.
abstract: PorB is a pan-Neisserial major outer membrane protein with a trimeric β-barrel structure. Each monomer presents eight periplasmic turns and eight surface exposed loop regions with sequence variability. PorB induces activation of host cell responses via a TLR2-dependent mechanism likely mediated by electrostatic interactions between TLR2 and PorB surface exposed loops. Variability in the loop amino acid sequence is known to influence cell responses to PorB in vitro, particularly for the residues in L5 and L7. In this work, the sequence of the porB gene and the electrostatic surface charges of PorB from 35 invasive meningococcal isolates belonging to the main clonal complexes identified in Italy and from five carriage genomes available on the website http://pubmlst.org/neisseria/ were examined. Analysis of the porB encoding regions from the invasive meningococci has identified four new alleles and a potential association between porB alleles, serogroup, and clonal complexes. Through computer-based modeling and analysis of the electrostatic surface charges of PorB from these strains, loop charge segregation between PorB from invasive serogroups B and C was observed. Specifically, loops 1, 4, and 7 were negatively charged and L2 and L8 were mostly neutral in serogroup B isolates, while an overall homogeneous positive surface charge was present in PorB from invasive serogroup C strains. A higher PorB sequence variability was observed among carriage genomes, and a general prevalence of negative loop surface charges. The surface charge differences in PorB from serogroups B and C invasive and carriage strains may, in part, influence the outcomes of Neisseriae interactions with host cells. © 2016 IUBMB Life, 68(6):488-495, 2016.
authors.affiliation: Department of Infectious, Parasitic & Immuno-Mediated Diseases, Istituto Superiore Di Sanità, Rome, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Stefanelli
authors.affiliation: Department of Infectious, Parasitic & Immuno-Mediated Diseases, Istituto Superiore Di Sanità, Rome, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Neri
authors.affiliation: Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Tanabe
authors.affiliation: Department of Infectious, Parasitic & Immuno-Mediated Diseases, Istituto Superiore Di Sanità, Rome, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Fazio
authors.affiliation: Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, MA, USA.
authors.forename: Paola
authors.initials: P
authors.lastname: Massari
mesh_terms: D000483:Alleles; D008958:Models, Molecular; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D018272:Porins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D065288:Serogroup
chemical_list: D018272:Porins; C057699:porin protein, Neisseria
keywords: Neisseria meningitides; PorB