title: Effectiveness of Periodic Treatment of Quercetin against Influenza A Virus H1N1 through Modulation of Protein Expression.
abstract: Kimchi, a traditional fermented food regularly consumed in Korea, contains various types of antimicrobial compounds. Among the tested compounds present in common spices used in Kimchi, quercetin showed the highest selectivity index against influenza A virus (IAV) H1N1. In this study, the effect of pretreatment and periodic treatment with quercetin against IAV in Madin-Darby canine kidney cells was observed. Compared to pretreatment, periodic treatment resulted in significantly higher cell viability but lower relative expression of the IAV PA gene and total apoptosis and cell death. To explain the mechanisms underlying the antiviral effects of quercetin treatment, a comparative proteomic analysis was performed in four samples (mock, quercetin-treated, IAV-infected, and quercetin-treated IAV-infected). Among the 220 proteins, 56 proteins were classified nonhierarchically into three clusters and were differentially modulated by quercetin treatment in IAV-infected cells. Post-translational modifications were identified in 68 proteins. In conclusion, periodic treatment with quercetin is effective in reducing IAV infection, and differentially regulates the expression of key proteins, including heat shock proteins, fibronectin 1, and prohibitin to reduce IAV replication.
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.|Bioenergy Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Bipin
authors.initials: B
authors.lastname: Vaidya
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Cho
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Kyung-Seo
authors.initials: KS
authors.lastname: Oh
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Song Hak
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Yeong O
authors.initials: YO
authors.lastname: Kim
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Jeong
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Thoa Thi
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Hygienic Safety and Analysis Center, World Institute of Kimchi , Gwangju 61755, South Korea.
authors.forename: Sung Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: In Seon
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Korea Basic Science Institute , Daejeon 34133, South Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Food Science and Technology, BK21 Plus Program, and Foodborne Virus Research Center, Chonnam National University , Gwangju 61186, South Korea.|Bioenergy Research Center, Chonnam National University , Gwangju 61186, South Korea.
authors.forename: Duwoon
authors.initials: D
authors.lastname: Kim
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004285:Dogs; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D007251:Influenza, Human / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D011794:Quercetin / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D011506:Proteins; D011794:Quercetin
keywords: heat shock protein; influenza A virus; post-translational modification; proteomics; quercetin