title: Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus).
abstract: The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3.
authors.affiliation: College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea - Incheon International Airport Regional Office, National Fishery Products Quality Management Service, Ministry of Oceans and Fisheries, Incheon 22382, Republic of Korea.
authors.forename: Jaeyoun
authors.initials: J
authors.lastname: Kang
authors.affiliation: College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Changnam
authors.initials: C
authors.lastname: Park
authors.affiliation: Ocean and Fisheries Research Institute, Jeju Special Self-Governing Province, Pyoseon-myeon, Segwipo-si, Jeju 63629, Republic of Korea.
authors.forename: Yeounghwan
authors.initials: Y
authors.lastname: Jang
authors.affiliation: College of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.affiliation: College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D019921:Biotinylation; D002241:Carbohydrates / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis; D004847:Epithelial Cells / Q000737:chemistry*; D005393:Fish Diseases / Q000175:diagnosis / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D005432:Flounder / Q000378:metabolism / Q000469:parasitology*; D016266:Food Parasitology; D005838:Genotype; D006790:Host-Parasite Interactions; D006984:Hypertrophy; D007124:Immunoenzyme Techniques; D037102:Lectins; D018482:Muscle, Skeletal / Q000737:chemistry / Q000469:parasitology* / Q000473:pathology; D055764:Myxozoa / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D010273:Parasitic Diseases, Animal / Q000175:diagnosis / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D016415:Sequence Alignment; D013379:Substrate Specificity
chemical_list: D002241:Carbohydrates; D004272:DNA, Mitochondrial; D037102:Lectins