title: Metallic/bimetallic magnetic nanoparticle functionalization for immobilization of α-amylase for enhanced reusability in bio-catalytic processes.
abstract: Novel magnetic nanoparticles coated with silica and gold were synthesized for immobilization of α-amylase enzyme and characterized with Fourier transform infrared spectroscopy, transmission electron microscopy. Effect of various limiting factors such as substrate concentration, temperature, and pH on the catalytic activity of enzyme was investigated. The optimum pH for free and immobilized enzyme was found unaffected (7.0), whereas optimum temperature for the enzyme activity was increased from 60°C for free enzyme to 80°C for immobilized counterpart. The gains in catalytic attributes concomitant to ease of recovery of the enzyme reflect the potential of the approach and the product to be useful for the enzymatic bioprocessing. The Michaelis-Menten constant (Km) value of the immobilized α-amylase was higher than that of free α-amylase, whereas maximum velocity (Vmax), and turn over number (Kcat), values were almost similar. Immobilized α-amylase maintained 60% of the enzyme activity even after recycling ten times.
authors.affiliation: National Agri-food Biotechnology Institute (NABI), C-127, I Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Singh
authors.affiliation: National Agri-food Biotechnology Institute (NABI), C-127, I Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India.
authors.forename: Kanak
authors.initials: K
authors.lastname: Rakshit
authors.affiliation: National Agri-food Biotechnology Institute (NABI), C-127, I Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Rathee
authors.affiliation: National Agri-food Biotechnology Institute (NABI), C-127, I Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India.
authors.forename: Stanzin
authors.initials: S
authors.lastname: Angmo
authors.affiliation: National Agri-food Biotechnology Institute (NABI), C-127, I Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India.
authors.forename: Shimayali
authors.initials: S
authors.lastname: Kaushal
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Garg
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Chung
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh 160014, India.
authors.forename: Rajat
authors.initials: R
authors.lastname: Sandhir
authors.affiliation: Center for Innovative and Applied Bioprocessing (CIAB), C-127, II Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India.
authors.forename: Rajender S
authors.initials: RS
authors.lastname: Sangwan
authors.affiliation: National Agri-food Biotechnology Institute (NABI), C-127, I Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India. Electronic address: nitin@nabi.res.in.
authors.forename: Nitin
authors.initials: N
authors.lastname: Singhal
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D055162:Biocatalysis; D000067493:Dynamic Light Scattering; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008670:Metals / Q000737:chemistry*; D059027:Recycling; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D008670:Metals; D012822:Silicon Dioxide; D000516:alpha-Amylases
keywords: Enzyme immobilization; Kinetics; Magnetic nanoparticles; α-Amylase