title: Fragile sites of 45S rDNA of Lolium multiflorum are not hotspots for chromosomal breakages induced by X-ray.
abstract: Sites of 45S rDNA of Lolium are regions denominated fragile sites (FSs), constituting regions slightly stained with DAPI due to increased DNA unpacking in metaphasic chromosomes. Considered to be fragile regions in the genome, the FSs might be more responsive to induced breaks and result in chromosomal fragments and rearrangements, unless repairing mechanisms such as recombination or de novo telomere formation play a role at the break site of the DNA. Thus, this study aimed at investigating if SFs from Lolium are hotspots for the occurrence of breakages induced by X-ray and if they are regions favorable to synthesize new telomeres, using Hordeum vulgare as a comparative model. Lolium multiflorum and H. vulgare seedlings were irradiated with 20 and 50 Gy X-ray and evaluated one day following the irradiation and at 7-days intervals for a 28-days period, using FISH technique with 45S rDNA and Arabidopsis-type telomere probes in order to investigate the presence of chromosomal breakages and new telomere formation. H. vulgare did not survive after a few days of irradiation due to the increased rate of abnormalities. L. multiflorum also exhibited chromosomal abnormalities following the exposure, yet over the 28-days trial it had a decrease in the chromosomal damage rate and formation of de novo telomere has not been detected along this time. Despite being considered to be fragile regions in the genome, the 45S rDNA sites of Lolium are not hotspots to chromosomal breakages after the induction of breakages.
authors.affiliation: Department of Biology, Federal University of Lavras - UFLA, Lavras, Minas Gerais, Brazil.
authors.forename: Laiane Corsini
authors.initials: LC
authors.lastname: Rocha
authors.affiliation: Embrapa Dairy Cattle/Embrapa Temperate Agriculture, Juiz de Fora/Pelotas, Minas Gerais State/Rio Grande do Sul State, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mittelmann
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, 06466, StadtSeeland, Germany. houben@ipk-gatersleben.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Houben
authors.affiliation: Department of Biology, Federal University of Lavras - UFLA, Lavras, Minas Gerais, Brazil. vhtechio@gmail.com.
authors.forename: Vânia Helena
authors.initials: VH
authors.lastname: Techio
mesh_terms: D019457:Chromosome Breakage; D043283:Chromosome Fragile Sites / Q000528:radiation effects*; D017343:Genes, Plant; D008129:Lolium / Q000166:cytology / Q000235:genetics* / Q000528:radiation effects; D008677:Metaphase; D018749:RNA, Plant / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D014965:X-Rays
chemical_list: D018749:RNA, Plant; D012335:RNA, Ribosomal; C088179:RNA, ribosomal, 45S
keywords: Barley; Chromosomal abnormalities; DNA repair; De novo telomere formation; Ionizing radiation; Ryegrass