title: Developing a fluorescence-coupled capillary electrophoresis based method to probe interactions between QDs and colorectal cancer targeting peptides.
abstract: As is well known, quantum dots (QDs) have become valuable probes for cancer imaging. In particular, QD-labeled targeting peptides are capable of identifying cancer or tumors cells. A new colorectal cancer targeting peptide, cyclo(1, 9)-CTPSPFSHC, has strong targeting ability and also shows great potential in the identification and treatment of colon cancer. Herein, we synthesized a dual functional polypeptide, cyclo(1, 9)-CTPSPFSHCD2 G2 DP9 G3 H6 (H6 -TCP), to investigate its interaction with QDs inside the capillary. Fluorescence-coupled CE was adopted and applied to characterize the self-assembly of H6 -TCP onto QDs. It was indicated that the formation of the assembly was affected by H6 -TCP/QD molar ratio and sampling time. This novel in-capillary assay greatly reduced the sample consumption and the detection time, which was beneficial for the environment. It is expected that this kind of detection method could find more applications to provide more useful information for cancer diagnosis and detection of harm and hazardous substances/organisms in the environment in the future.
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Ding
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Minli
authors.initials: M
authors.lastname: Fu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.|Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Linhong
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore.
authors.forename: Li-Qian
authors.initials: LQ
authors.lastname: Gao
mesh_terms: D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D006639:Histidine; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry*; D009842:Oligopeptides; D010455:Peptides / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry
chemical_list: C471213:His-His-His-His-His-His; D015335:Molecular Probes; D009842:Oligopeptides; D010455:Peptides; D006639:Histidine
keywords: CE; Quantum dots; Self-assembly; Targeting peptide