title: Whole Genome Sequencing-Based Molecular Epidemiologic Analysis of Autochthonous Dengue Virus Type 1 Strains Circulating in Japan in 2014.
abstract: Cases of autochthonous infections of dengue virus type 1 (DENV-1) were detected in Japan after a 70-year period devoid of dengue outbreaks. We previously showed that E gene sequences are identical in 11 of the 12 DENV-1 strains autochthonous to Japan. However, the E sequence represents only 14% of the DENV-1 genome. In the present study, we have sequenced the entire genome of 6 autochthonous DENV-1 strains that were isolated from patients during the 2014 outbreak. Sequencing of 5 Yoyogi group strains with identical E sequences and 1 Shizuoka strain with a different E sequence revealed that the first Yoyogi group strain differed from the Shizuoka strain by 18 amino acid residues. Furthermore, 2 Yoyogi group strains had different genomic sequences while the other 3 had identical genomes. Phylogenetic analyses indicated that the Hyogo strain, a Yoyogi group strain, was the first to diverge from the other 4 Yoyogi group strains. The E gene sequence of the Yoyogi group strains exhibits the highest homology to those of the strains isolated in Malaysia and Singapore between 2013 and 2014. The patient infected with the Hyogo strain visited Malaysia before the onset of dengue fever, suggesting that this was a case of dengue infection imported from Malaysia.
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tajima
authors.forename: Eri
authors.initials: E
authors.lastname: Nakayama
authors.forename: Akira
authors.initials: A
authors.lastname: Kotaki
authors.forename: Meng Ling
authors.initials: ML
authors.lastname: Moi
authors.forename: Makiko
authors.initials: M
authors.lastname: Ikeda
authors.forename: Kazumi
authors.initials: K
authors.lastname: Yagasaki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Saito
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Shibasaki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
mesh_terms: D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D017720:Molecular Epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology