title: Tropoelastin enhances nitric oxide production by endothelial cells.
abstract: This study aimed to characterize the role of tropoelastin in eliciting a nitric oxide response in endothelial cells.
  
  
  
  Nitric oxide production in cells was quantified following the addition of known nitric oxide synthase pathway inhibitors such as LNAME and 1400W. The effect of eNOS siRNA knockdowns was studied using western blotting and assessed in the presence of PI3K-inhibitor, wortmannin.
  
  
  
  Tropoelastin-induced nitric oxide production was LNAME and wortmannin sensitive, while being unaffected by treatment with 1400W.
  
  
  
  Tropoelastin acts through a PI3K-specific pathway that leads to the phosphorylation of eNOS to enhance nitric oxide production in endothelial cells. This result points to the benefit of the use of tropoelastin in vascular applications, where NO production is a characteristic marker of vascular health.
authors.affiliation: School of Life & Environmental Biosciences, University of Sydney, Sydney, NSW 2006, Australia.|Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Matti A
authors.initials: MA
authors.lastname: Hiob
authors.affiliation: Centre for Heart Lung Innovation, St Paul's Hospital, Vancouver, BC, V6Z1Y6, Canada.|Department of Anesthesiology, Pharmacology & Therapeutics, University of British Columbia, Vancouver, Canada.
authors.forename: Andy E
authors.initials: AE
authors.lastname: Trane
authors.affiliation: School of Life & Environmental Biosciences, University of Sydney, Sydney, NSW 2006, Australia.|Sydney Medical School, University of Sydney, NSW 2006, Australia.|The Heart Research Institute, Sydney, NSW, 2042, Australia.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Wise
authors.affiliation: Centre for Heart Lung Innovation, St Paul's Hospital, Vancouver, BC, V6Z1Y6, Canada.|Department of Anesthesiology, Pharmacology & Therapeutics, University of British Columbia, Vancouver, Canada.
authors.forename: Pascal N
authors.initials: PN
authors.lastname: Bernatchez
authors.affiliation: School of Life & Environmental Biosciences, University of Sydney, Sydney, NSW 2006, Australia.|Charles Perkins Centre, University of Sydney, Sydney, NSW 2006, Australia.|Bosch Institute, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D014333:Tropoelastin / Q000378:metabolism*; D000077191:Wortmannin
chemical_list: D000578:Amidines; D000730:Androstadienes; D001596:Benzylamines; D004791:Enzyme Inhibitors; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014333:Tropoelastin; D000077191:Wortmannin; C104820:N-(3-(aminomethyl)benzyl)acetamidine
keywords: endothelial cells; nitric oxide; nitric oxide synthase; phosphatidylinositol 3-kinase; small interfering RNA; tropoelastin