title: Identification and functional analysis of interferon regulatory factor 3 in Lateolabrax maculatus.
abstract: The interferon (IFN) regulatory factor 3 (IRF3) is a member of the IFN regulatory transcription factor family, which binds to the IFN-stimulated response element (ISRE) within the promoter of IFN genes and IFN-stimulated genes. In this study, the IRF3 cDNA of sea perch Lateolabrax maculatus (SpIRF3) was identified, which contained 1781 bp with an open reading frame of 1398 bp that coded a 465 amino acid protein. The SpIRF3 protein shared conserved characterizations with its homologues and displayed the conserved DNA-binding domain, IRF association domain, serine-rich C-terminal domain, and tryptophan residue cluster. Phylogenetic analysis illustrated that SpIRF3 belonged to the IRF3 subfamily. Subcellular localization analysis showed that SpIRF3 mainly resided in the cytoplasm without stimuli but translocated into nuclei in the presence of poly I:C. Real-time PCR data indicated that SpIRF3 was transcriptionally up-regulated by poly I:C stimulation in various organs. Moreover, reporter assay revealed that SpIRF3 functioned as a modulator in triggering the IFN response by inducing the activity of IFN and ISRE-containing promoter. These data revealed that SpIRF3 was a potential molecule in the IFN immune defense system against viral infection.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Xiao-Wu
authors.initials: XW
authors.lastname: Chen
authors.affiliation: Department of Surgical Oncology and Institute of Clinical Medicine, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China; Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, TX 75390-9133, USA.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Wang
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address: yhbi@shou.edu.cn.
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Bi
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, TX 75390-9133, USA. Electronic address: gu-yifeng@hotmail.com.
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Gu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D050835:Interferon Regulatory Factors / Q000235:genetics / Q000378:metabolism*; D007372:Interferons / Q000378:metabolism*; D010471:Perches / Q000276:immunology*; D010802:Phylogeny; D011070:Poly I-C / Q000276:immunology; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport; D020218:Response Elements / Q000235:genetics
chemical_list: D030161:Avian Proteins; D029941:Fish Proteins; C094532:IRF-3 protein, Gallus gallus; D050835:Interferon Regulatory Factors; D007372:Interferons; D011070:Poly I-C
keywords: IRF3 (Interferon regulatory factor 3); ISGs (IFN-stimulated genes); ISRE (Interferon-stimulated response element); Lateolabrax maculatus