title: Preparation, characterization, and cytotoxic effects of liposomal nanoparticles containing cisplatin: an in vitro study.
abstract: Cisplatin is a chemotherapeutic agent used for treating various malignancies. The study aimed to prepare pegylated liposomal cisplatin and evaluate its efficacy against human breast cancer cell line MCF-7. Drug-loaded nanoparticles were synthesized by reverse phase evaporation technique. The study is highlighted by extensive characterization of nanoparticles in terms of nanoparticle morphology, type of drug entrapment, cisplatin retention capability, and cytotoxicity effects. The size, size distribution, and zeta potential of nanodrug were estimated 142 nm, 0.33, and -22 mV, respectively. Drug-loading efficiency was equal to 48% that occurred physically. Furthermore, high retention capability (39% of drug was released after 72 h) with significantly enhanced cytotoxicity of nanodrug (1.75 times more than the standard drug) confirmed the potency of liposomal nanoparticles as proper cisplatin carrier.
authors.affiliation: Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Donya
authors.initials: D
authors.lastname: Poy
authors.affiliation: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran. azimakbarzadeh21@gamil.com.
authors.forename: Azim
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: Department of Microbiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Hasan
authors.initials: H
authors.lastname: Ebrahimi Shahmabadi
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza, Iran.
authors.forename: Meysam
authors.initials: M
authors.lastname: Ebrahimifar
authors.affiliation: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Farhangi
authors.affiliation: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Farahnak Zarabi
authors.affiliation: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Akbari
authors.affiliation: Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Saffari
authors.affiliation: Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran. rahel_siamy@yahoo.com.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Siami
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D002945:Cisplatin / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity*; D010316:Particle Size
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D002945:Cisplatin
keywords: atomic force microscopy; cisplatin; cytotoxicity; drug delivery; pegylated liposome