title: Growth inhibition of human gastric adenocarcinoma cells in vitro by STO-609 is independent of calcium/calmodulin-dependent protein kinase kinase-beta and adenosine monophosphate-activated protein kinase.
abstract: Adenosine monophosphate (AMP)-activated protein kinase is a recently identified downstream target of calcium/calmodulin-dependent protein kinase kinase-beta, and is involved in the regulation of cell metabolism and cell proliferation. STO-609 is a selective antagonist of calcium/calmodulin-dependent protein kinase kinase-beta. In the present study, we found that STO-609 suppressed AMP-activated protein kinase activity, reduced expression of Akt and ERK, and increased cell apoptosis in SNU-1 and N87 cells but not normal gastric epithelial cells (CCL-241). Interestingly, we found such effects of STO-609 on gastric cancer cells were not affected after the knock-down of CaMKK-β and AMPK. In conclusion, STO-609 is an effective cytotoxic agent for gastric adenocarcinoma in vivo.
authors.affiliation: Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Dacheng
authors.initials: D
authors.lastname: Wen
authors.affiliation: Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory for Molecular and Chemical Genetics of Critical Human Diseases of Jilin Province, The Second Hospital of Jilin University Changchun, P. R. China.
authors.forename: Longfei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Tianzhou
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University Changchun 130041, P. R. China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin UniversityChangchun 130041, P. R. China; Department of General Surgery, China-Japan Union Hospital of Jilin UniversityChangchun, P. R. China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Fang
keywords: AMP-activated protein kinase; Adenocarcinoma; apoptosis; calcium/calmodulin-dependent protein kinase kinase-beta; gastric carcinoma; short hairpin RNA