title: Ball mill assisted rapid mechanochemical extraction method for natural products from plants.
abstract: A ball mill assisted mechanochemical extraction method was developed to extract compounds of natural product (NP) from plant using ionic liquid (IL). A small volume ball mill, also known as PastPrep(®) Homogenizer, which is often used for high-speed lysis of biological samples and for other applications, was used to dramatically increase the speed, completeness and reproducibility of the extraction process at room temperature to preserve the chemical integrity of the extracted compounds. In this study, tanshinones were selected as target compounds to evaluate the performance of this extraction method. Factors affecting the extraction efficiency, such as the duration, IL concentration and solid/liquid ratio were systematically optimized using the response surface methodology. Under the optimized conditions, the described method was more efficient and much faster than the conventional extraction methods such as methanol based ultrasound assisted extraction (UAE) and heat reflux extraction (HRE) that consumes a lot more organic solvent. In addition, the natural products of interest were enriched by anion metathesis of ionic liquids, combining extraction and preconcentration in the same process. The extractant was analyzed by HPLC and LC-MS. The reproducibility (RSD, n=5), correlation coefficient (r(2)) of the calibration curve, and the limit of detection, were determined to be in the range of 4.7-5.2%, 0.9992-0.9995, and 20-51ng/mL, respectively.
authors.affiliation: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: biwentao@njnu.edu.cn.
authors.forename: Wentao
authors.initials: W
authors.lastname: Bi
authors.affiliation: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Huang
authors.affiliation: Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China; Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada. Electronic address: david-chen@njnu.edu.cn.
authors.forename: David Da Yong
authors.initials: Dda Y
authors.lastname: Chen
mesh_terms: D045784:Abietanes / Q000302:isolation & purification*; D001688:Biological Products / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D052578:Ionic Liquids; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D012997:Solvents; D000069453:Ultrasonic Waves
chemical_list: D045784:Abietanes; D001688:Biological Products; D052578:Ionic Liquids; D010936:Plant Extracts; D012997:Solvents; C021751:tanshinone
keywords: Ball mill assisted mechanochemical extraction; Ionic liquid; Natural product from plant; Sample preparation; Tanshinones