title: Increased expression of endocytosis-Related proteins in rat hippocampus following 10-day electroconvulsive seizure treatment.
abstract: Although electroconvulsive therapy (ECT) is clinically used for severe depression and drug-resistant Parkinson's disease, its exact biological background and mechanism have not yet been fully elucidated. Two potential explanations have been presented so far to explain the increased neuroplastic and resilient profiles of multiple ECT administrations. One is the alteration of central neurotransmitter receptor densities and the other is the expressional upregulation of brain derived neurotrophic factor in various brain regions with enhanced hippocampal neurogenesis and mossy fiber sprouting. In the present report, western blot analyses revealed significantly upregulated expression of various endocytosis-related proteins following 10-day electroconvulsive seizure (ECS) treatment in rat hippocampal homogenates and hippocampal lipid raft fractions extracted using an ultracentrifugation procedure. Upregulated proteins included endocytosis-related scaffolding proteins (caveolin-1, flotillin-1, and heavy and light chains of clathrin) and small GTPases (Rab5, Rab7, Rab11, and Rab4) specifically expressed on various types of endosomes. Two scaffolding proteins, caveolin-1 and flotillin-1, were also increased in the lipid raft fraction. Together with our previous finding of increased autophagy-related proteins in the hippocampal region, the present results suggest membrane trafficking machinery is enhanced following 10-day ECS treatment. We consider that the membrane trafficking machinery that transports functional proteins in the neuronal cells and from or into the synaptic membranes is one of the new candidates supporting the cellular and behavioral neuroplastic profiles of ECS treatments in animal experiments and ECT administrations in clinical settings.
authors.affiliation: Department of Psychiatry, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Enomoto
authors.affiliation: Division of Behavioral Sciences, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: Department of Psychiatry, National Defense Medical College, Tokorozawa, Saitama, Japan; Department of Psychiatry, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan. Electronic address: nibrin@ndmc.ac.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Nibuya
authors.affiliation: Department of Psychiatry, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Toda
authors.affiliation: Department of Psychiatry, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Aihide
authors.initials: A
authors.lastname: Yoshino
authors.affiliation: Department of Psychiatry, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Suzuki
authors.affiliation: Department of Chest Surgery, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.affiliation: Department of Radiology, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D002966:Clathrin / Q000378:metabolism; D004565:Electroconvulsive Therapy; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D033921:Vesicular Transport Proteins / Q000378:metabolism
chemical_list: D002966:Clathrin; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; D020559:Monomeric GTP-Binding Proteins
keywords: Antidepressant; Autophagy; Electroconvulsive seizure; Endocytosis; Neural plasticity; Resilience; Stress