title: Beclin 1 promotes apoptosis and decreases invasion by upregulating the expression of ECRG4 in A549 human lung adenocarcinoma cells.
abstract: Although Beclin 1 has been demonstrated to exert an important role in cell autophagy during carcinogenesis, its biological function in lung cancer has yet to be fully elucidated. A previous study by our laboratory identified that knockdown of Beclin 1 promoted cell growth and inhibited apoptosis in the A549 lung cancer cell line. In the present study, a Beclin 1 lentiviral expression vector was constructed, and an A549 cell line was established with a steady expression of Beclin 1. Furthermore, the effect of Beclin 1 overexpression on cell invasion and apoptosis, changes in the activities of the apoptosis‑associated caspases‑3 and ‑9, and the overexpression of esophageal cancer‑related gene 4 (ECRG4) were examined. The results demonstrated that the overexpression of Beclin 1 in A549 cells reduced cell invasion by Matrigel invasion assay and promoted apoptosis by flow cytometric analysis (P<0.01) compared with Lenex‑packaged lentiviral particles and non‑transfected control groups. Furthermore, the overexpression of Beclin 1 in A549 cells increased the activities of caspases‑3 and ‑9 and the expression of ECRG4 (P<0.01) compared with Lenex‑packaged lentiviral particles and non‑transfected control groups. In conclusion, the overexpression of Beclin 1 promoted apoptosis and decreased invasion by upregulating the expression of ECRG4 in A549 lung adenocarcinoma cells. Therefore, the selection of Beclin l as a target for gene therapy represents a more effective method for the treatment of lung cancer.
authors.affiliation: Department of Medical Oncology, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Wang
authors.affiliation: Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Hongkun
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Medical Oncology, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430023, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Life Sciences College, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Weida
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430023, P.R. China.
authors.forename: Guihong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Basic Medical College, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Medical Oncology, Zhengzhou University People's Hospital (Henan Provincial People's Hospital), Zhengzhou, Henan 450003, P.R. China.
authors.forename: Linwei
authors.initials: L
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis / Q000235:genetics*; D000071186:Beclin-1 / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics*; D014161:Transduction, Genetic; D025521:Tumor Suppressor Proteins
chemical_list: D000071186:Beclin-1; C476999:ECRG4 protein, human; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D053148:Caspase 3; D053453:Caspase 9