title: Effect of starch on the mechanical and in vitro properties of collagen-hydroxyapatite sponges for applications in dentistry.
abstract: This study sought to improve the mechanical and blood-absorbing properties of collagen sponges, while keeping them compressible, by incorporating blended hydroxyapatite (HA)-starch. Results were compared with CollaPlug(®) (pure collagen). The elastic modulus increased from 1.5±0.2kPa for CollaPlug(®) to 49±8kPa for sponges with composition 1:4:10 (collagen:HA:starch, by weight). The modified microstructure and surface area provided by the starch granules on the sponges improved cell viability. Sponges with composition 1:4:10 maintained their blood-clotting capability with almost no change from 5 to 15min after contact with blood, while CollaPlug(®) diminished to about half its capacity to absorb blood and form clots. Incorporation of HA-starch into the sponges with composition of 1:4:10, increased the elastic modulus of the collagen-HA sponges, making them more structurally robust. The viability of cells and the blood-clotting capability increased with starch incorporation.
authors.affiliation: Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: acastro@cideteq.mx.
authors.forename: Ana B
authors.initials: AB
authors.lastname: Castro-Ceseña
authors.affiliation: Biomedical Innovation Department, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, C.P. 22860, Ensenada, Baja California, México. Electronic address: tcamacho@ciatej.mx.
authors.forename: Tanya A
authors.initials: TA
authors.lastname: Camacho-Villegas
authors.affiliation: Biomedical Innovation Department, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, C.P. 22860, Ensenada, Baja California, México. Electronic address: plugo@cicese.edu.mx.
authors.forename: Pavel H
authors.initials: PH
authors.lastname: Lugo-Fabres
authors.affiliation: Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: eevdokim@ucsd.edu.
authors.forename: Ekaterina E
authors.initials: EE
authors.lastname: Novitskaya
authors.affiliation: Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA 92093, USA; Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: jmckittrick@eng.ucsd.edu.
authors.forename: Joanna
authors.initials: J
authors.lastname: McKittrick
authors.affiliation: Biomedical Innovation Department, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, C.P. 22860, Ensenada, Baja California, México. Electronic address: alicea@cicese.mx.
authors.forename: Alexei
authors.initials: A
authors.lastname: Licea-Navarro
mesh_terms: D003813:Dentistry; D017886:Durapatite / Q000737:chemistry*; D055119:Elastic Modulus / Q000187:drug effects; D006801:Humans; D013213:Starch / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D013213:Starch; D017886:Durapatite
keywords: Blood-clotting; CollaPlug(®); Collagen; Dental filler; Sponge; Starch