title: Slx4 scaffolding in homologous recombination and checkpoint control: lessons from yeast.
abstract: Homologous recombination-mediated DNA repair is essential for maintaining genome integrity. It is a multi-step process that involves resection of DNA ends, strand invasion, DNA synthesis and/or DNA end ligation, and finally, the processing of recombination intermediates such as Holliday junctions or other joint molecules. Over the last 15 years, it has been established that the Slx4 protein plays key roles in the processing of recombination intermediates, functioning as a scaffold to coordinate the action of structure-specific endonucleases. Recent work in budding yeast has uncovered unexpected roles for Slx4 in the initial step of DNA-end resection and in the modulation of DNA damage checkpoint signaling. Here we review these latest findings and discuss the emerging role of yeast Slx4 as an important coordinator of DNA damage signaling responses and a regulator of multiple steps in homologous recombination-mediated repair.
authors.affiliation: Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: José R
authors.initials: JR
authors.lastname: Cussiol
authors.affiliation: Department of Biosciences, University of Milan, 20133, Milan, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Dibitetto
authors.affiliation: Department of Biosciences, University of Milan, 20133, Milan, Italy.
authors.forename: Achille
authors.initials: A
authors.lastname: Pellicioli
authors.affiliation: Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, USA. mbs266@cornell.edu.
authors.forename: Marcus B
authors.initials: MB
authors.lastname: Smolka
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D059447:Cell Cycle Checkpoints; D018797:Cell Cycle Proteins / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D059765:Homologous Recombination; D010766:Phosphorylation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C097185:DPB11 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D004706:Endodeoxyribonucleases; C505436:SLX4 protein, S cerevisiae
keywords: Checkpoint; Dpb11; Homologous recombination; Resection; Slx4