title: Enzymatic hydrolysis of starry triggerfish (Abalistes stellaris) muscle using liver proteinase from albacore tuna (Thunnus alalunga).
abstract: Proteinases from liver extract from albacore tuna (Thunnus alalunga) were used to produce protein hydrolysate from starry triggerfish (Abalistes stellaris) muscle. Hydrolysis conditions for preparing protein hydrolysate from starry triggerfish muscle were optimized. Enzyme level, reaction time and fish muscle/buffer ratio significantly affected the hydrolysis (p < 0.05). Optimum conditions for triggerfish muscle hydrolysis were 5.5 % liver extract, 40 min reaction time and fish muscle/buffer ratio of 1:3 (w/v). The freeze-dried protein hydrolysate was characterized with respect to chemical composition, amino acid composition and color. The product contained 91.73 % protein, 2.04 % lipid and 6.48 % ash. The protein hydrolysate exhibited high amount of essential amino acids (45.62 %). It was light yellow in color (L (*) = 82.94, a (*) = 0.84, b (*) = 22.83). The results indicate that the extract from liver of albacore tuna could be used to produce fish protein hydrolysate and protein hydrolysate from starry triggerfish muscle may potentially serve as a good source of desirable peptide and amino acids.
authors.affiliation: Biotechnology Program, Faculty of Technology and Community Development, Phatthalung Campus, Thaksin University, Phatthalung, 93210 Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Sripokar
authors.affiliation: Department of Food Technology, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, 80160 Thailand.
authors.forename: M
authors.initials: M
authors.lastname: Chaijan
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Benjakul
authors.affiliation: Laboratory of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611 Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kishimura
authors.affiliation: Department of Food Science and Technology, Faculty of Technology and Community Development, Phatthalung Campus, Thaksin University, Phatthalung, 93210 Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Klomklao
keywords: Industrial application; Protein hydrolysate; Proteinases; Tuna