title: Protective effects of hyperoside against H2O2-induced apoptosis in human umbilical vein endothelial cells.
abstract: The vascular endothelium is important in the physiological homeostasis of blood vessels. Increasing evidence demonstrates that oxidative stress‑induced endothelial damage is involved in the pathogenesis of several cardiovascular diseases, including atherosclerosis. Hyperoside, one of major active components from Apocynum venetum L. (Luo‑Bu‑Ma), which is a traditional Chinese herbal medicine commonly used for the prevention of cardiovascular diseases, exhibits diverse bioactivities, including anti‑inflammatory and antioxidant effects. In the present study, the protective effects of hyperoside against hydrogen peroxide (H2O2)‑induced apoptosis of human umbilical vein endothelial cells (HUVECs) were investigated. The results demonstrated that hyperoside significantly prevented the loss of cell viability, the increase of endothelial Ca2+ content and apoptosis in H2O2‑induced HUVECs. Additionally, reverse transcription-polymerase chain reaction and western blot analysis revealed that hyperoside significantly decreased the mRNA expression levels of B‑cell lymphoma (Bcl)‑2 associated X protein (Bax), cleaved caspase‑3 and phosphorylated‑p38, while increasing the mRNA expression of Bcl‑2 in H2O2‑induced HUVECs. The present findings suggested that hyperoside has protective effects against H2O2‑induced apoptosis in HUVECs and serves a key role in the prevention of cardiovascular diseases.
authors.affiliation: Institute of Chinese Medicine Prescriptions, Shanxi Province Academy of Traditional Chinese Medicine, Taiyuan, Shanxi 030012, P.R. China.
authors.forename: Xu-Liang
authors.initials: XL
authors.lastname: Hao
authors.affiliation: Institute of Chinese Medicine Prescriptions, Shanxi Province Academy of Traditional Chinese Medicine, Taiyuan, Shanxi 030012, P.R. China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Kang
authors.affiliation: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Jian-Kuan
authors.initials: JK
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: En-Li
authors.initials: EL
authors.lastname: Liu
authors.affiliation: Institute of Chinese Medicine Prescriptions, Shanxi Province Academy of Traditional Chinese Medicine, Taiyuan, Shanxi 030012, P.R. China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C021304:hyperoside; D011794:Quercetin; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases; D053148:Caspase 3; D002118:Calcium