title: N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors level in hfat-1 transgenic pigs.
abstract: BACKGROUND
  The consumption of n-3 polyunsaturated fatty acids (PUFAs) is important to human health, especially in cases of cardiovascular disease. Although beneficial effects of n-3 PUFAs have been observed in a number of studies, the mechanisms involved in these effects have yet to be discovered.
  
  
  METHODS
  We generated hfat-1 transgenic pigs with traditional somatic cell nuclear transfer (SCNT) technology. The fatty acid composition in ear tissue of pigs were detected with gas chromatography. The cholesterol, triglycerides (TAG) and inflammation mediators in circulation were investigated.
  
  
  RESULTS
  The hfat-1 transgenic pigs were developed which accumulate high levels of n-3 PUFAs than wild-types pigs. Gas chromatography results demonstrated that the total n-3 PUFAs in the ear tissues of the transgenic founders were 2-fold higher than the wild-type pigs. A lipid analysis demonstrated that the levels of TAG in the transgenic pigs were decreased significantly. The basal levels of the inflammation mediators tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) in transgenic pigs were inhibited markedly compared with the wild-type pigs.
  
  
  CONCLUSIONS
  These results suggest that n-3 PUFAs accumulation in vivo may have beneficial effects on vascular and hfat-1 transgenic pigs may be a useful tool for investigating the involved mechanisms.
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Daxin
authors.initials: D
authors.lastname: Pang
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yuan
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Ren
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Model Animal Engineering Research Center, College of Animal Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China. xiaochuntang@jlu.edu.cn.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015820:Cadherins / Q000235:genetics*; D018932:Chemokine CCL2 / Q000235:genetics; D002784:Cholesterol / Q000097:blood / Q000235:genetics; D008076:Cholesterol, HDL / Q000097:blood / Q000235:genetics; D015525:Fatty Acids, Omega-3 / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000178:diet therapy* / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D034421:Sus scrofa; D014280:Triglycerides / Q000097:blood* / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D015820:Cadherins; D018932:Chemokine CCL2; D008076:Cholesterol, HDL; C502403:FAT1 protein, human; D015525:Fatty Acids, Omega-3; D015850:Interleukin-6; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D002784:Cholesterol
keywords: Inflammation; Transgenic pig- fat-1; Triglyceride; n-3 polyunsaturated fatty acids