title: Changing the content of phenolic compounds as the response of blackcurrant (Ribes nigrum L.) leaves after blackcurrant leaf midge (Dasineura tetensi Rübs.) infestation.
abstract: Blackcurrant leaf midge (Dasineura tetensi) is one of the most common pests of blackcurrant (Ribes nigrum). The aim of this study was to investigate changes in the content of phenolic compounds in the leaves damaged by the larvae of this pest. Additionally, susceptibility of different blackcurrant cultivars to the midge attack was investigated. Qualitative and quantitative analyses of control and pest-infested blackcurrant leaves were performed using LC-PDA-QTOF/MS and UPLC-PDA-FL systems. A total of 39 types of phenolic compounds were identified in blackcurrant leaf extracts and they included 3 flavan-3-ols, 14 hydroxycinnamic acid derivatives, and 22 flavonols. Feeding of blackcurrant leaf midge on blackcurrant leaves lowered the content of leaf polyphenolic compounds. The greatest differences in polyphenolics between control and infected leaves were observed in 'Ruben', 'Fariegh', 'Foxendown', 'Ores', 'Ben Hope', 'Ben Connan' and 'Tisel' cultivars that were probably highly susceptible to the pest attack. In the other cultivars: 'Ben Finlay', 'Polares', 'Tiben', and 'Gofert' the differences in phenolics content were less pronounced, so they were probably less susceptible to D. tetensi attack. Plant polyphenolic compounds was strongly involved in pathogen-plant interaction, and their accumulation significantly decreased as a result of the pathogen attack.
authors.affiliation: Research Institute of Horticulture, Department of Pomology; St. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Piotrowski
authors.affiliation: Wroclaw University of Environmental and Life Sciences, Department of Fruit, Vegetable and Grain Technology, St. J. Chełmoński 37, 51-630 Wroclaw, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Oszmiański
authors.affiliation: Wroclaw University of Environmental and Life Sciences, Department of Fruit, Vegetable and Grain Technology, St. J. Chełmoński 37, 51-630 Wroclaw, Poland. Electronic address: aneta.wojdylo@up.wroc.pl.
authors.forename: Aneta
authors.initials: A
authors.lastname: Wojdyło
authors.affiliation: Research Institute of Horticulture, Department of Pomology; St. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland.
authors.forename: Barbara H
authors.initials: BH
authors.lastname: Łabanowska
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004175:Diptera / Q000502:physiology*; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000378:metabolism* / Q000469:parasitology; D059808:Polyphenols / Q000032:analysis; D031965:Ribes / Q000378:metabolism* / Q000469:parasitology
chemical_list: D010636:Phenols; D059808:Polyphenols
keywords: Blackcurrant; Dasineura tetensi; Flavonoids; LC-PDA-QTOF/MS and UPLC-PDA-FL; Phenolic acids; Phenolic compounds; Ribes nigrum