title: Derivation of porcine pluripotent stem cells for biomedical research.
abstract: Pluripotent stem cells including embryonic stem cells (ESCs), embryonic germ cells (EGCs), and induced pluripotent stem cells (iPSCs) are capable of self-renew and limitlessly proliferating in vitro with undifferentiated characteristics. They are able to differentiate in vitro, spontaneously or responding to suitable signals, into cells of all three primary germ layers. Consequently, these pluripotent stem cells will be valuable sources for cell replacement therapy in numerous disorders. However, the promise of human ESCs and EGCs is cramped by the ethical argument about destroying embryos and fetuses for cell line creation. Moreover, there are still carcinogenic risks existing toward the goal of clinical application for human ESCs, EGCs, and iPSCs. Therefore, a suitable animal model for stem cell research will benefit the further development of human stem cell technology. The pigs, on the basis of their similarity in anatomy, immunology, physiology, and biochemical properties, have been wide used as model animals in the study of various human diseases. The development of porcine pluripotent stem cell lines will hold the opportunity to provide an excellent material for human counterpart to the transplantation in biomedical research and further development of cell-based therapeutic strategy.
authors.affiliation: Institute of Biomedical Science, National Sun Yat-sen University, Taiwan.
authors.forename: Yow-Ling
authors.initials: YL
authors.lastname: Shiue
authors.affiliation: Division of Physiology, Livestock Research Institute, Council of Agriculture, Taiwan.
authors.forename: Jenn-Rong
authors.initials: JR
authors.lastname: Yang
authors.affiliation: Division of Physiology, Livestock Research Institute, Council of Agriculture, Taiwan.
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Liao
authors.affiliation: Division of Physiology, Livestock Research Institute, Council of Agriculture, Taiwan.
authors.forename: Ting-Yung
authors.initials: TY
authors.lastname: Kuo
authors.affiliation: Department of Medical Research, Buddhist Tzu Chi General Hospital, Taiwan.
authors.forename: Chia-Hsin
authors.initials: CH
authors.lastname: Liao
authors.affiliation: Division of Physiology, Livestock Research Institute, Council of Agriculture, Taiwan.
authors.forename: Ching-Hsun
authors.initials: CH
authors.lastname: Kang
authors.affiliation: Southern Taiwan University, Taiwan.
authors.forename: Chein
authors.initials: C
authors.lastname: Tai
authors.affiliation: Department of Animal Science, University of California, Davis, USA.
authors.forename: Gary B
authors.initials: GB
authors.lastname: Anderson
authors.affiliation: Division of Physiology, Livestock Research Institute, Council of Agriculture, Taiwan; Southern Taiwan University, Taiwan; National Chung Kung Univeristy, Taiwan. Electronic address: lrchen@mail.tlri.gov.tw.
authors.forename: Lih-Ren
authors.initials: LR
authors.lastname: Chen
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000941:ethics / Q000379:methods*; D003584:Cytological Techniques; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D057905:Stem Cell Research / Q000941:ethics*; D013552:Swine
keywords: Animal model; Biomedical research; Embryonic stem cell; Induced pluripotent stem cell; Pig