title: Cre Recombinase and Other Tyrosine Recombinases.
abstract: Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the predictable modification of genomes as they enable precise genome editing in heterologous hosts. These enzymes are ubiquitous in eubacteria, prevalent in archaea and temperate phages, present in certain yeast strains, but barely found in higher eukaryotes. As tools they find increasing use for the generation and systematic modification of genomes in a plethora of organisms. If applied in host organisms, they enable precise DNA cleavage and ligation without the gain or loss of nucleotides. Criteria directing the choice of the most appropriate T-SSR system for genetic engineering include that, whenever possible, the recombinase should act independent of cofactors and that the target sequences should be long enough to be unique in a given genome. This review is focused on recent advancements in our mechanistic understanding of simple T-SSRs and their application in developmental and synthetic biology, as well as in biomedical research.
authors.affiliation: Department of Developmental, Molecular & Chemical Biology, Tufts University School of Medicine , Boston, Massachusetts 02111, United States.
authors.forename: Gretchen
authors.initials: G
authors.lastname: Meinke
authors.affiliation: Department of Developmental, Molecular & Chemical Biology, Tufts University School of Medicine , Boston, Massachusetts 02111, United States.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bohm
authors.affiliation: Heinrich Pette Institute, Leibniz Institute for Experimental Virology , 20251 Hamburg, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Hauber
authors.affiliation: Structural Bioinformatics, BIOTEC TU Dresden , 01307 Dresden, Germany.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Pisabarro
authors.affiliation: Medical Systems Biology, UCC, Medical Faculty Carl Gustav Carus TU Dresden , 01307 Dresden, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Buchholz
mesh_terms: D004247:DNA / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000737:chemistry / Q000378:metabolism*; D019426:Integrases / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D014443:Tyrosine; D004247:DNA; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C077570:Site-specific recombinase