title: Detection and differentiation of wild-type and vaccine strains of canine distemper virus by a duplex reverse transcription polymerase chain reaction.
abstract: Canine distemper virus (CDV) is the cause of canine distemper (CD) which is a severe and highly contagious disease in dogs. In the present study, a duplex reverse transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of CDV. Four primers were designed to detect and discriminate the two viruses by generating 638- and 781-bp cDNA products, respectively. Furthermore, the duplex RT-PCR method was used to detect 67 field samples suspected of CD from Guangdong province in China. Results showed that, 33 samples were to be wild-type-like. The duplex RT-PCR method exhibited high specificity and sensitivity which could be used to effectively detect and differentiate wild-type and vaccine CDV, indicating its use for clinical detection and epidemiological surveillance.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tian He District, Guangzhou 510642, China; ; Department of Animal Science, College of Yingdong Agricultural Science and Engineering, Shaoguan University, Shaoguan 512005, China;
authors.forename: X Y
authors.initials: XY
authors.lastname: Dong
authors.affiliation: MSc in Virus, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tian He District, Guangzhou 510642, China;
authors.forename: W H
authors.initials: WH
authors.lastname: Li
authors.affiliation: BSc in Virus, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tian He District, Guangzhou 510642, China;
authors.forename: J L
authors.initials: JL
authors.lastname: Zhu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tian He District, Guangzhou 510642, China;
authors.forename: W J
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: MSc in Vaccine, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tian He District, Guangzhou 510642, China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Zhao
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tian He District, Guangzhou 510642, China;
authors.forename: Y W
authors.initials: YW
authors.lastname: Luo
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, South China Agricultural University, Tian He District, Guangzhou 510642, China;
authors.forename: J D
authors.initials: JD
authors.lastname: Chen
keywords: Canine distemper virus; Differentiation; Duplex RT-PCR; Sensitivity; Specificity