title: Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing.
abstract: Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas. We obtained the complete genome of a CVA isolate (CVA-TA) using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The newly identified 5'-untranslated region (5'-UTR) from CVA-TA may form additional hairpin and loop structures to stabilize the CVA genome.
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, Shandong, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, Washington State University, Pullman, Washington, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, USA.
authors.forename: Weizhen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Horticulture, Washington State University, Pullman, Washington, USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Dhingra
authors.affiliation: Department of Plant Pathology, Washington State University, Pullman, Washington, USA hrp@wsu.edu qzliu001@126.com.
authors.forename: Hanu R
authors.initials: HR
authors.lastname: Pappu
authors.affiliation: Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, Shandong, China hrp@wsu.edu qzliu001@126.com.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Liu