title: The Effect of the Molecular Architecture on the Antioxidant Properties of Chitosan Gallate.
abstract: To elucidate the structure-antioxidant activity relationships of chitosan gallate (CG), a series of CG derivatives with different degrees of substitution (DS's) and molecular weights (MWs) were synthesized from chitosan (CS) and gallic acid (GA) via a free radical graft reaction. A higher MW led to a lower DS of CG. The structures of CG were characterized by FT-IR and ¹H NMR, and results showed that GA was mainly conjugated to the C-2 and C-6 positions of the CS chain. The antioxidant activity (the DPPH radical scavenging activity and reducing power) were enhanced with an increased DS and a decreased MW of CG. A correlation between antioxidant activities and the DS and MW of CG was also established. In addition, a suitable concentration (0~250 μg/mL) of CG with different MWs (32.78~489.32 kDa) and DS's (0~92.89 mg·GAE/g CG) has no cytotoxicity. These results should provide a guideline to the application of CG derivatives in food or pharmacology industries.
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R &amp; D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China. chwu0283@163.com.|Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan. chwu0283@163.com.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wu
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R &amp; D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China. zacamille@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, the University of Melbourne, Parkville, Victoria 3010, Australia. zhongxiang.fang@unimelb.edu.au.
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Fang
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R &amp; D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China. yqhu@zju.edu.cn.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R &amp; D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China. chenshiguo210@163.com.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
authors.affiliation: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan. sugawara@kais.kyoto-u.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sugawara
authors.affiliation: College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R &amp; D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China. psu@zju.edu.cn.
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; C547764:chitosan gallate; D017665:Hydroxyl Radical; D005707:Gallic Acid; D048271:Chitosan
keywords: antioxidant activity; chitosan gallate; gallic acid; grafting; molecular architecture