title: Sister chromatid exchange test in river buffalo lymphocytes treated in vitro with furocoumarin extracts.
abstract: Furocoumarin extracts from Psoralea morisiana, the endemic Sardinian legume species, were tested for their mutagenic potential on river buffalo blood cells. The results obtained performing the sister chromatid exchange (SCE) test in blood cultures of five river buffalo calves (exposure to furocoumarins for 72h) and five cows (exposure to furocoumarins for 3h, in the absence and presence of S9 metabolic activator) are reported. Significant differences in mean values of SCEs were observed in cells of calves compared to control cells (unexposed), but no differences in SCE mean values were found between treated and untreated cells of cows in the presence or absence of S9. SCE mean values were much higher in cells of cows (exposed and control) than in cells of calves. Indeed, in calf cells, SCE mean values/cell (±SD) were 6.66±2.45 in the control and 7.63±3.01, 9.03±3.90, 9.53±3.60 and 9.99±3.41 in treated cells at 50, 100, 200 and 400 µg/ml of furocoumarin extracts, respectively. In cow cells, grown in presence of S9, SCE mean values/cell were 11.49±4.78 and 11.65±5.19 in treated cells at 100 and 200 µg/ml of furocoumarins and 11.66±5.45 in the control. In cow cells grown in absence of S9, SCE mean values were 11.81±6.14 in the control and 12.35±7.09 and 12.01±5.43, respectively, in the presence of 100 and 200 µg/ml of furocoumarins. Despite their higher SCE values in the absence of S9, no statistically significant differences were found when these values were compared with those shown in presence of S9, suggesting no mutagenic action of furocoumarins in cows, at the doses used in this study.
authors.affiliation: National Research Council (CNR), Institute for Animal Production Systems in Mediterranean Environments (ISPAAM), Laboratory of Animal Cytogenetics and Genetics, Via Argine 1085, 80147 Naples, Italy, alessandra.iannuzzi@cnr.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Iannuzzi
authors.affiliation: National Research Council (CNR), Institute for Animal Production Systems in Mediterranean Environments (ISPAAM), Laboratory of Animal Cytogenetics and Genetics, Via Argine 1085, 80147 Naples, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Perucatti
authors.affiliation: National Research Council (CNR), Institute for Animal Production Systems in Mediterranean Environments (ISPAAM), Laboratory of Animal Cytogenetics and Genetics, Via Argine 1085, 80147 Naples, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Genualdo
authors.affiliation: National Research Council (CNR), Institute for Animal Production Systems in Mediterranean Environments (ISPAAM), Laboratory of Animal Cytogenetics and Genetics, Via Argine 1085, 80147 Naples, Italy, Dept. Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Via Leonardo Da Vinci, 44, 10095 Grugliasco Turin, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Pauciullo
authors.affiliation: CNR-ISPAAM, UOS-Sassari, Trav. La Crucca, 3, 07100 Sassari, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Melis
authors.affiliation: CNR-ISPAAM, UOS-Sassari, Trav. La Crucca, 3, 07100 Sassari, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Porqueddu
authors.affiliation: CNR-ICB, UOS-Sassari, Trav. La Crucca, 3, 07100 Sassari, Italy and.
authors.forename: Mauro
authors.initials: M
authors.lastname: Marchetti
authors.affiliation: Department of Science for Nature and Environmental Resources (DIPNET), University of Sassari, Via Muroni, 23A, 07100 Sassari, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Usai
authors.affiliation: National Research Council (CNR), Institute for Animal Production Systems in Mediterranean Environments (ISPAAM), Laboratory of Animal Cytogenetics and Genetics, Via Argine 1085, 80147 Naples, Italy.
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Iannuzzi
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D011564:Furocoumarins / Q000494:pharmacology / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology / Q000633:toxicity; D029907:Psoralea / Q000737:chemistry; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: D011564:Furocoumarins; D009153:Mutagens