title: A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression.
abstract: The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain reaction (RT-PCR) and clone sequencing methods. The novel GALNTL5 isoform was derived from the complete transcript, GALNTL5-complete, via alternative splicing (AS). The pattern of the splice variant was exon skipping. Bovine GALNTL5 transcripts were expressed in the testis, as demonstrated by RT-PCR. The expression levels of both transcripts were higher in adult testes than in calf testes (P < 0.05). In addition, prediction analysis showed that the GALNTL5-AS transcript only encoded 122 amino acids and lost its glycosyltransferase 1 and Gal/GalNAc-T motifs, which may result in a dysfunctional protein compared with the predominant transcript GALNTL5-complete. This study improves our understanding of the bovine GALNTL5 gene function during bull sperm formation.
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.|College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Ju
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Huang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Wang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: G W
authors.initials: GW
authors.lastname: Yang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Zhong
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
mesh_terms: D017398:Alternative Splicing; D020816:Amino Acid Motifs; D000818:Animals; D002417:Cattle; D005091:Exons; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D017350:N-Acetylgalactosaminyltransferases