title: Human Stem Cell-Derived Skin Progenitors Produce Alpha 2-HS Glycoprotein (Fetuin): A Revolutionary Cosmetic Ingredient.
abstract: These studies were designed to determine the effect of stem cell-derived skin lineage precursor secretions on the intrinsic and extrinsic symptoms of human skin aging.<br /> Human stem cells cultivated in balanced conditions were differentiated into skin lineage precursors, and shown to secrete large amounts of fetuin as well as multiple growth factors beneficial for human skin development and maintenance. The cell secretions were incorporated in two simple cosmetic formulations (serum and lotion) and investigated in an IRB-approved 12-week human trial that included 25 subjects in each group. Subjects were examined at 2, 4, 8, and 12 weeks by a dermatologist to evaluate safety, trans-epidermal water loss, wrinkles, firmness, radiance, texture, softness, and overall appearance. A sub-group of subjects from each group consented for biopsies for histological analyses.<br /> Protein analyses in the cell secretions revealed a high concentration of the multifunctional alpha 2-HS glycoprotein (fetuin) along with a multitude of protein factors involved in the development and maintenance of healthy human skin. Clinical investigation demonstrated significant amelioration of the clinical signs of intrinsic and extrinsic skin aging, findings that were confirmed by significant changes in skin morphology, filaggrin, aquaporin 3, and collagen I content.<br /> Our data strongly support our hypothesis that cosmetic application of stem cell-derived skin lineage precursor secretions containing fetuin and growth factors beneficial for human skin development and maintenance, positively influence intrinsic and extrinsic aging. <br /><br /> <em>J Drugs Dermatol. </em>2016;15(5):583-598.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Nistor
authors.forename: Aleksandra J
authors.initials: AJ
authors.lastname: Poole
authors.forename: Zoe
authors.initials: Z
authors.lastname: Draelos
authors.forename: Mary
authors.initials: M
authors.lastname: Lupo
authors.forename: Thomas
authors.initials: T
authors.lastname: Tzikas
authors.forename: Jerome H
authors.initials: JH
authors.lastname: Liu
authors.forename: Hans S
authors.initials: HS
authors.lastname: Keirstead
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D003358:Cosmetics / Q000008:administration & dosage*; D000091344:Filaggrin Proteins; D006801:Humans; D015595:Skin Aging / Q000187:drug effects* / Q000502:physiology; D063465:Skin Cream / Q000008:administration & dosage*; D013234:Stem Cells / Q000378:metabolism*; D060749:alpha-2-HS-Glycoprotein / Q000008:administration & dosage* / Q000378:metabolism*
chemical_list: C556354:AHSG protein, human; D003358:Cosmetics; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D060749:alpha-2-HS-Glycoprotein