title: Potential of MALDI imaging for the toxicological evaluation of environmental pollutants.
abstract: Risk assessment related to the exposure of humans to chemicals released into the environment is a major concern of our modern societies. In this context, toxicology plays a crucial role to characterize the effects of this exposure on health and identify the targets of these molecules. MALDI imaging mass spectrometry (IMS) is an enabling technology for biodistribution studies of chemicals. Although the majority of published studies are presented in a pharmacological context, the concepts discussed in this review can be applied to the toxicological evaluation of chemicals released into the environment. The major asset of IMS is the simultaneous localization and identification of a parent molecule and its metabolites without labeling and without any prior knowledge. Quantification methods developed in IMS are presented with application to an environmental pollutant. IMS is effective in the localization of chemicals and endogenous species. This opens unique perspectives for the discovery of molecular alterations in metabolites and protein biomarkers that could help for a better understanding of toxicity mechanisms. Distribution studies of agrochemicals in plants by IMS can contribute to a better understanding of their mode of action and to a more effective use of these chemicals, avoiding the current concern of environmental damage.
authors.affiliation: Protim, Inserm U1085, IRSET, Campus de Beaulieu, Rennes Cedex F-35042, France; Université de Rennes I, Campus de Beaulieu, Rennes Cedex F-35042, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Lagarrigue
authors.affiliation: Departments of Biochemistry, Chemistry, Pharmacology and Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
authors.affiliation: Protim, Inserm U1085, IRSET, Campus de Beaulieu, Rennes Cedex F-35042, France; Université de Rennes I, Campus de Beaulieu, Rennes Cedex F-35042, France. Electronic address: charles.pineau@inserm.fr.
authors.forename: Charles
authors.initials: C
authors.lastname: Pineau
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004785:Environmental Pollutants / Q000493:pharmacokinetics* / Q000633:toxicity*; D006801:Humans; D010944:Plants / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants
keywords: Environmental pollutants; Imaging; MALDI; Mass spectrometry; Toxicology