title: Biological correlation between glucose transporters, Ki-67 and 2-deoxy-2-[18F]-fluoro-D-glucose uptake in diffuse large B-cell lymphoma and natural killer/T-cell lymphoma.
abstract: The purpose of this study was to investigate the association between cellular 2-deoxy-2-[18F]-fluoro-D-glucose ((18)F-FDG) uptake and the expression of several subtypes of glucose transporters (GLUT) and Ki-67 in diffuse large B-cell lymphoma (DLBCL) and natural killer (NK)/T-cell lymphoma (NKTCL). Cell lines were histologically determined to be DLBCL (Raji cells) and NKTCL (Daudi cells), and uptake after pretreatment with (18)F-FDG was determined. Real-time polymerase chain reaction was performed to detect the expression levels of GLUTs 1, 2, 3, 4, and 7 and Ki-67, and to evaluate their association with (18)F-FDG uptake in DLBCL and NKTCL cells. The uptake rates of (18)F-FDG ranged from 18 to 46% (average 30 ± 10.20%) in Raji cells and 25 to 48% (average 35.6 ± 7.57%) in Daudi cells. In DLBCL cells, the expression levels of GLUTs 1, 3, and 7 were significantly correlated with cellular (18)F-FDG uptake rates (Spearman's rank correlation coefficient of 0.667, 0.516, and 0.468, respectively; P < 0.05). In NKTCL cells, the expression levels of GLUTs 1 and 3 were observed to be significantly correlated with cellular (18)F-FDG uptake rates (Spearman's rho of 0.756 and 0.498, respectively; P < 0.05). Ki-67 played no role in (18)F-FDG uptake in Raji or Daudi cells. In conclusion, the data acquired through this preliminary study indicate that GLUT 1 and GLUT 3 contribute to 18F-FDG uptake in DLBCL and NKTCL.
authors.affiliation: Department of Nuclear Medicine, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, Suzhou, China.|Department of Nuclear Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Zhai
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D005947:Glucose / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000378:metabolism; D016403:Lymphoma, Large B-Cell, Diffuse / Q000378:metabolism*; D016399:Lymphoma, T-Cell / Q000378:metabolism*; D055611:Natural Killer T-Cells / Q000378:metabolism
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D019394:Ki-67 Antigen; D019788:Fluorodeoxyglucose F18; D005947:Glucose