title: Mechanically Stiff, Zinc Cross-Linked Nanocomposite Scaffolds with Improved Osteostimulation and Antibacterial Properties.
abstract: Nanocomposite scaffolds are studied widely due to their resemblance with the natural extracellular matrix of bone; but their use as a bone tissue engineered scaffold is clinically hampered due to low mechanical stiffness, inadequate osteoconduction, and graft associated infections. The purpose of the current study was the development of a mechanically stiff nanocomposite scaffold using biodegradable gellan and xanthan polymers reinforced with bioglass nanoparticles (nB) (Size: 20-120 nm). These nanocomposite scaffolds were cross-linked with zinc sulfate ions to improve their osteoconduction and antibacterial properties for the regeneration of a functional bone. The compressive strength and modulus of the optimized nanocomposite scaffold (1% w/v polymer reinforced with 4%w/v nB nanoparticles, cross-linked with 1.5 mM zinc sulfate) was 1.91 ± 0.31 MPa and 20.36 ± 1.08 MPa, respectively, which was comparable to the trabecular bone and very high compared to nanocomposite scaffolds reported in earlier studies. Further, in vitro simulated body fluid (SBF) study suggested deposition of biomimetic apatite on the surface of zinc cross-linked nanocomposite scaffolds confirming their bioactivity. MG 63 osteoblast-like cells cultured with the nanocomposite scaffolds responded to matrix stiffness with better adhesion, spreading and cellular interconnections compared to the polymeric gellan and xanthan scaffolds. Incorporation of bioglass nanoparticles and zinc cross-linker in nanocomposite scaffolds demonstrated 62% increment in expression of alkaline phosphatase activity (ALP) and 150% increment in calcium deposition of MG 63 osteoblast-like cells compared to just gellan and xanthan polymeric scaffolds. Furthermore, zinc cross-linked nanocomposite scaffolds significantly inhibited the growth of Gram-positive Bacillus subtilis (70% reduction) and Gram-negative Escherichia coli (81% reduction) bacteria. This study demonstrated a facile approach to tune the mechanical stiffness, bioactivity, osteoconduction potential and bacteriostatic properties of scaffolds, which marked it as a potential bone tissue engineered scaffold.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay , Mumbai 400076, India.
authors.forename: Rekha R
authors.initials: RR
authors.lastname: Sehgal
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay , Mumbai 400076, India.
authors.forename: Edmund
authors.initials: E
authors.lastname: Carvalho
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay , Mumbai 400076, India.
authors.forename: Rinti
authors.initials: R
authors.lastname: Banerjee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D015032:Zinc
keywords: antibacterial property; bioglass nanoparticles; bone tissue engineering; gellan; nanocomposite scaffolds; zinc