title: [Human Umbilical Cord-derived Mesenchymal Stem Cells Secrete Interleukin-6 to Influence Differentiation of Leukemic Cells].
abstract: OBJECTIVE
  To investigate the effect of human umbilical cord-derived mesenchymal stem cells (UC-MSC) on the differentiation of leukemic cells.
  
  
  METHODS
  The co-culture system of UC-MSC with acute promyelocytic leukemic cell line NB4 cells was constructed in vitro,and the differentiation status of the leukemic cells was assessed by cell morphology,nitroblue tetrazolium reduction test,and cell surface differentiation marker CD11b.
  
  
  RESULTS
  UC-MSC induced the granulocytic differentiation of NB4 cells. When UC-MSC and a small dose of all-trans retinoic acid were applied together,the differentiation-inducing effect was enhanced in an additive manner. Interleukin (IL)-6Ra neutralization attenuated differentiation and exogenous IL-6-induced differentiation of leukemic cells.
  
  
  CONCLUSIONS
  UC-MSC can promotd granulocytic differentiation of acute promyelocytic leukemia cells by way of IL-6 and presented additive effect when combined with a small dose of all-trans retinoic acid.
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,CAMS and PUMC,Tianjin 300020,China;
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,CAMS and PUMC,Tianjin 300020,China;
authors.forename: Feng-xia
authors.initials: FX
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,CAMS and PUMC,Tianjin 300020,China;
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,CAMS and PUMC,Tianjin 300020,China;
authors.forename: Fang-yun
authors.initials: FY
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,CAMS and PUMC,Tianjin 300020,China;
authors.forename: Ying
authors.initials: Y
authors.lastname: Chi
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,CAMS and PUMC,Tianjin 300020,China;
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Hospital of Blood Diseases,CAMS and PUMC,Tianjin 300020,China;
authors.forename: Zhong-chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D002454:Cell Differentiation; D045744:Cell Line, Tumor; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014470:Umbilical Cord / Q000166:cytology
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D014212:Tretinoin