title: Smart Nanotransformers with Unique Enzyme-Inducible Structural Changes and Drug Release Properties.
abstract: We previously reported a high aspect ratio peptide nanofiber that could be effectively delivered to tumors with minimal nonspecific uptake by other organs. The peptidic nature offers the design flexibility of smart formulation with unique responsiveness. Two new formulations that behave congruously as nanotransformers (NTFs) are reported herein. NTF1 and NTF2 could biomechanically remodel upon enzyme activation to generate a degradable and an aggregable effect, respectively, within the lysosomal compartment. These NTFs were further evaluated as carriers of mertansine (DM1), a microtubule inhibitor. DM1-loaded NTF1 could be degraded by cathepsin B (CathB) to release the same active metabolite, as previously described in the lysosomal degradation of antibody-DM1 conjugate. In contrast, CathB only partially digested DM1-loaded NTF2 and induced aggregate formation to become a storage reservoir with slow payload release property. The DM1-loaded NTF1 exhibited a comparable cytotoxicity to the free drug and was more effective than the NTF2 formulation in eradicating triple negative breast cancer. Our data suggested that biological transformers with distinct enzyme-induced structural changes and payload release profiles could be designed for the intracellular delivery of cytotoxic and imaging agents.
authors.affiliation: Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine , 413 East 69th Street, New York, New York 10021, United States.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Bellat
authors.affiliation: Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine , 413 East 69th Street, New York, New York 10021, United States.
authors.forename: Hyun Hee
authors.initials: HH
authors.lastname: Lee
authors.affiliation: Department of Medicine, Weill Cornell Medicine , 425 East 61st Street, New York, New York 10065, United States.
authors.forename: Linda
authors.initials: L
authors.lastname: Vahdat
authors.affiliation: Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine , 413 East 69th Street, New York, New York 10021, United States.
authors.forename: Benedict
authors.initials: B
authors.lastname: Law
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000201:enzymology; D002401:Cathepsin B / Q000378:metabolism*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D005260:Female; D006801:Humans; D008453:Maytansine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D050257:Tubulin Modulators / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D004337:Drug Carriers; D009842:Oligopeptides; D050257:Tubulin Modulators; D008453:Maytansine; D002401:Cathepsin B