title: Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri.
abstract: Phosphorus is an essential element for life on earth and is also important for modern agriculture, which is dependent on inorganic fertilizers from phosphate rock. Polyphosphate is a biological polymer of phosphate residues, which is accumulated in organisms during the biological wastewater treatment process to enhance biological phosphorus removal. Here, we investigated the relationship between polyphosphate accumulation and electron-dense bodies in the green alga Parachlorella kessleri. Under sulfur-depleted conditions, in which some symporter genes were upregulated, while others were downregulated, total phosphate accumulation increased in the early stage of culture compared to that under sulfur-replete conditions. The P signal was detected only in dense bodies by energy dispersive X-ray analysis. Transmission electron microscopy revealed marked ultrastructural variations in dense bodies with and without polyphosphate. Our findings suggest that the dense body is a site of polyphosphate accumulation, and P. kessleri has potential as a phosphate-accumulating organism.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.|CREST, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Ota
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Yoshihara
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.|CREST, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Yamazaki
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takeshita
authors.affiliation: Bioimaging Center, Graduate School of Frontier Science, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Hirata
authors.affiliation: Hitachi High-Technologies Corporation, Science &Medical Systems Business Group, Nishi-shinbashi, Tokyo, 105-8717, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Konomi
authors.affiliation: Center for Omics and Bioinformatics, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8561, Japan.
authors.forename: Kenshiro
authors.initials: K
authors.lastname: Oshima
authors.affiliation: Center for Omics and Bioinformatics, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8561, Japan.
authors.forename: Masahira
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Institute of Microbiology, CAS, Centre Algatech, Laboratory of Cell Cycles of Algae, Třeboň, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Bišová
authors.affiliation: Institute of Microbiology, CAS, Centre Algatech, Laboratory of Cell Cycles of Algae, Třeboň, Czech Republic.
authors.forename: Vilém
authors.initials: V
authors.lastname: Zachleder
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, 277-8562, Japan.|CREST, Japan Science and Technology Agency, Tokyo, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawano
mesh_terms: D000460:Chlorophyta / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D004583:Electrons; D021621:Imaging, Three-Dimensional; D008055:Lipids / Q000737:chemistry*; D008954:Models, Biological; D010710:Phosphates / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013194:Staining and Labeling; D013455:Sulfur / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
chemical_list: D008055:Lipids; D010710:Phosphates; D011122:Polyphosphates; D013455:Sulfur