title: Generation of induced pluripotent stem cells from human mesenchymal stem cells of parotid gland origin.
abstract: The technology to reprogram human somatic cells to pluripotent state allows the generation of patient-specific induced pluripotent stem cells (iPSCs) and holds a great promise for regenerative medicine and autologous transplantation. Here we, for the first time, identified mesenchymal stem cells isolated from parotid gland (hPMSCs) as a suitable candidate for iPSC production. In the present study, hPMSCs were isolated from parotid gland specimens in patients with squamous cell carcinoma of the oral cavity. The mesenchymal stem cell properties of cultured hPMSCs were confirmed by expression of surface markers and induced differentiation into osteogenic, chondrogenic and adipogenic cell lineages. hPMSCs were then reprogrammed to pluripotent cells by episomal vector-mediated transduction of reprogramming factors (OCT3/4, SOX2, KLF4, c-MYC, LIN28 and TP53 shRNA). The resulting hPMSC-iPSCs showed similar characteristics as human embryonic stem cells (ESCs) with regard to morphology, pluripotent markers, global gene expression, and methylation status of pluripotent cell-specific genes OCT4 and NANOG. These hPMSC-iPSCs were able to differentiate into cells of all three germ layers both in vitro and in vivo. Our results indicate that hPMSCs could be an alternative cell source for generation of iPSCs and have the potential to be used in cell-based regenerative medicine.
authors.affiliation: Department of Stomatology, Beijing Friendship Hospital, Capital Medical University Beijing, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Stomatology, Beijing Friendship Hospital, Capital Medical University Beijing, PR China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Stomatology, Beijing Friendship Hospital, Capital Medical University Beijing, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Meng
authors.affiliation: Department of Stomatology, Beijing Friendship Hospital, Capital Medical University Beijing, PR China.
authors.forename: Bianhong
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Stomatology, Beijing Friendship Hospital, Capital Medical University Beijing, PR China.
authors.forename: Jinghong
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Beijing Cellapy Biotechnology Co., LTD Beijing, PR China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Stem Cell Research Center and Department of Cell Biology, School of Basic Medical Sciences, Peking University Beijing, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
keywords: Induced pluripotent stem cell; mesenchymal stem cell; parotid gland; pluripotency; regenerative medicine