title: Effect of hypoxia on UDP-glucuronosyl transferase mRNA expression in human hepatocarcinoma functional liver celL4 cell line.
abstract: Although hypoxic conditions have been reported to affect the expression levels of various enzymes like cytochrome P450, the effect of hypoxia for UDP-glucuronosyl transferase (UGT) expression has been unclear. We evaluated the mRNA expression of UGTs (UGT1A1·1A6·1A9·2B7) in a functional liver cell-4 (FLC-4) cell line by three-dimensional culture under hypoxic conditions (37 °C, 1% O₂, 5% CO₂) fo 7 days. The mRNA expression of UGT1A1·1A6·1A9·2B7 decreased significantly after 3 days and that of UGT1A1·1A6·1A9 decreased significantly after 7 days. Hypoxic conditions affect the expression levels of UGT enzymes, thus the adjustment of dosage and interval should be considered in drug therapy that metabolized by UGT.
authors.forename: R
authors.initials: R
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Matsura
authors.forename: R
authors.initials: R
authors.lastname: Kamiya
authors.forename: C
authors.initials: C
authors.lastname: Oishi
authors.forename: Y
authors.initials: Y
authors.lastname: Kagawa
authors.forename: F
authors.initials: F
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ijiri
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D014453:Glucuronosyltransferase