title: IL-17 induces EMT via Stat3 in lung adenocarcinoma.
abstract: Epithelial-mesenchymal transition (EMT) plays a vital role in lung inflammatory diseases, including lung cancer. However, the role and mechanism of action of the proinflammatory cytokine IL-17 in EMT in lung adenocarcinoma remain unresolved. In our study, we discovered that the expression of N-cadherin, Vimentin, Snail1, Snail2, and Twist1 was positively correlated with IL-17 expression, while E-cadherin expression was negatively correlated with IL-17 expression in human lung adenocarcinoma tissues. Moreover, we confirmed that IL-17 promoted EMT in A549 and Lewis lung carcinoma (LLC) cells in vitro by upregulating N-cadherin, Vimentin, Snail1, Snail2, and Twist1 expression and downregulating E-cadherin expression. Stat3 was activated in IL-17-treated A549 and LLC cells, and Stat3 inhibition or siRNA knockdown notably reduced IL-17-induced EMT in A549 and LLC cells. Thus, IL-17 promotes EMT in lung adenocarcinoma via Stat3 signaling; these observations suggest that targeting IL-17 and EMT are potential novel therapeutic strategies for lung cancer.
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Jieli
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Jinshuo
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Duan
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Hu
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Tao
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Respiratory Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology 1277 Jiefang Avenue, Wuhan 430022, Hubei, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jin
keywords: IL-17; Snail1; Snail2; Stat3; Twist1; lung adenocarcinoma