title: Immunomodulation with Self-Crosslinked Polyelectrolyte Multilayer-Based Coatings.
abstract: This study aims to design an optimal polyelectrolyte multilayer film of poly-l-lysine (PLL) and hyaluronic acid (HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of anti-inflammatory cytokines (IL1-RA and CCL18) and the decrease of pro-inflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties.
authors.affiliation: INSERM UMR 1121 , 11 rue Humann, 67085 Strasbourg, France.|Faculté de Chirurgie Dentaire, Fédération de Médecine Translationnelle de Strabourg, Fédération des Matériaux et Nanosciences d'Alsace, Université de Strasbourg , 3 rue Sainte Elisabeth, 67000 Strasbourg, France.
authors.forename: Helena
authors.initials: H
authors.lastname: Knopf-Marques
authors.affiliation: Division of Immunology, School of Life Sciences, Faculty of Medicine and Life Sciences, University of Nottingham, Queen's Medics, al Centre , Nottingham, NG7 2UH, United Kingdom.
authors.forename: Sonali
authors.initials: S
authors.lastname: Singh
authors.affiliation: Division of Immunology, School of Life Sciences, Faculty of Medicine and Life Sciences, University of Nottingham, Queen's Medics, al Centre , Nottingham, NG7 2UH, United Kingdom.
authors.forename: Su Su
authors.initials: SS
authors.lastname: Htwe
authors.affiliation: Contipro a.s., Dolni Dobrouc 401 561 02 Dolni Dobrouc, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Wolfova
authors.affiliation: Contipro a.s., Dolni Dobrouc 401 561 02 Dolni Dobrouc, Czech Republic.
authors.forename: Radovan
authors.initials: R
authors.lastname: Buffa
authors.affiliation: Laboratoire de Chimie Physique et Microbiologie pour l'Environnement CNRS UMR7564 , 405 rue de Vandoeuvre, 54600 Villers-les-Nancy, France.
authors.forename: Jalal
authors.initials: J
authors.lastname: Bacharouche
authors.affiliation: Laboratoire de Chimie Physique et Microbiologie pour l'Environnement CNRS UMR7564 , 405 rue de Vandoeuvre, 54600 Villers-les-Nancy, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Francius
authors.affiliation: INSERM UMR 1121 , 11 rue Humann, 67085 Strasbourg, France.|Faculté de Chirurgie Dentaire, Fédération de Médecine Translationnelle de Strabourg, Fédération des Matériaux et Nanosciences d'Alsace, Université de Strasbourg , 3 rue Sainte Elisabeth, 67000 Strasbourg, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Voegel
authors.affiliation: INSERM UMR 1121 , 11 rue Humann, 67085 Strasbourg, France.|Faculté de Chirurgie Dentaire, Fédération de Médecine Translationnelle de Strabourg, Fédération des Matériaux et Nanosciences d'Alsace, Université de Strasbourg , 3 rue Sainte Elisabeth, 67000 Strasbourg, France.|Institut Charles Sadron, CNRS UPR 22 , 23 rue du Lœss, 67034 Strasbourg, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Schaaf
authors.affiliation: Division of Immunology, School of Life Sciences, Faculty of Medicine and Life Sciences, University of Nottingham, Queen's Medics, al Centre , Nottingham, NG7 2UH, United Kingdom.
authors.forename: Amir M
authors.initials: AM
authors.lastname: Ghaemmaghami
authors.affiliation: INSERM UMR 1121 , 11 rue Humann, 67085 Strasbourg, France.|Protip Medical, 8 Place de l'Hôpital, 67000 Strasbourg, France.
authors.forename: Nihal Engin
authors.initials: NE
authors.lastname: Vrana
authors.affiliation: INSERM UMR 1121 , 11 rue Humann, 67085 Strasbourg, France.|Faculté de Chirurgie Dentaire, Fédération de Médecine Translationnelle de Strabourg, Fédération des Matériaux et Nanosciences d'Alsace, Université de Strasbourg , 3 rue Sainte Elisabeth, 67000 Strasbourg, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lavalle
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry*; D016207:Cytokines / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D056747:Immunomodulation / Q000187:drug effects*; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000071228:Polyelectrolytes / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D003432:Cross-Linking Reagents; D016207:Cytokines; D000071228:Polyelectrolytes; D006820:Hyaluronic Acid