title: E-Prostanoid 2 Receptor Overexpression Promotes Mesenchymal Stem Cell Attenuated Lung Injury.
abstract: Mesenchymal stem cells (MSCs) represent a promising approach for the treatment of acute respiratory distress syndrome (ARDS). However, their low efficiency in homing to injured lung tissue limits their therapeutic effect. Prostaglandin E2 (PGE2) biosynthesis substantially enhances the inflammatory response of the tissue. Moreover, it also facilitates the migration of MSCs by activating the E-prostanoid 2 (EP2) receptor in vitro. Given these observations, it would seem reasonable that PGE2 might act as a chemokine to promote the migration of MSCs through activation of the EP2 receptor. Herein, we confirmed that PGE2 was significantly increased in lung tissue as a result of stimulation by LPS. In addition, we constructed a lentiviral vector carrying the EP2 gene, which was successfully transduced into MSCs (MSCs-EP2). Near-infrared imaging and immunofluorescence showed that compared with MSCs-GFP, MSCs-EP2 significantly enhanced MSC homing to injured lung tissue. Moreover, the diminished amounts of Evans blue in homogeneous lung parenchyma in vivo indicated, in comparison with MSCs-GFP, that MSCs-EP2 significantly decreased LPS-induced pulmonary vascular permeability. In addition, administration of MSCs-EP2 largely decreased the levels of interleukin-1β and tumor necrosis factor-α compared with that observed after administration of MSCs-GFP at both 24 and 72 hr. Our results suggested that treatment with MSCs-EP2 markedly enhanced MSC homing to damaged lung tissue and, in addition, improved both lung inflammation and permeability. Thus, MSCs and EP2 combination gene therapy could markedly facilitate MSC homing to areas of inflammation, representing a novel strategy for MSC-based gene therapy in inflammatory diseases.
authors.affiliation: Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
authors.forename: Jibin
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
authors.forename: Xiuping
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
authors.forename: Haibo
authors.initials: H
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D015316:Genetic Therapy; D008070:Lipopolysaccharides / Q000633:toxicity; D055370:Lung Injury / Q000209:etiology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000235:genetics*; D014161:Transduction, Genetic
chemical_list: D008070:Lipopolysaccharides; D058307:Receptors, Prostaglandin E, EP2 Subtype