title: Molecular characterization of Orf virus in goats in Gabon, Central Africa.
abstract: BACKGROUND
  Orf or contagious ecthyma is a zoonotic viral infection with a potential serious health threat for the small ruminants industry as well as humans. It is currently emerging in new territories.
  
  
  RESULTS
  Eight suspected clinical cases of pustular dermatitis in goats occurred in the rural area of Tebe, in south-eastern Gabon, in January 2013. The orf virus (ORFV) was detected by high-throughput sequencing on sera, buccal swabs and scab pool samples. It was confirmed in six out of eight sick goats by using specific PCR targeting the major envelope protein (B2L) and the orf virus interferon resistance (VIR) genes. Phylogenetic analysis revealed that the Gabonese strain and South Korean strains evolved from a common ancestor, suggesting an Asian origin of the ORFV' Gabonese strain.
  
  
  CONCLUSIONS
  This study provides the molecular detection of the ORFV strain involved in the cases of pustular dermatitis in goats and highlights its circulation in Gabon.
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon. gael_maganga@yahoo.fr.|Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), Franceville, Gabon. gael_maganga@yahoo.fr.
authors.forename: Gael D
authors.initials: GD
authors.lastname: Maganga
authors.affiliation: French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Maisons-Alfort, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Relmy
authors.affiliation: French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Maisons-Alfort, France.
authors.forename: Labib
authors.initials: L
authors.lastname: Bakkali-Kassimi
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon.
authors.forename: Barthélémy
authors.initials: B
authors.lastname: Ngoubangoye
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon.
authors.forename: Thierry
authors.initials: T
authors.lastname: Tsoumbou
authors.affiliation: Institut Pasteur, Plate-forme Génomique - Pôle Biomics, 28 rue du Dr. Roux, 75724, Paris, France.
authors.forename: Christiane
authors.initials: C
authors.lastname: Bouchier
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon.
authors.forename: Nadine
authors.initials: N
authors.lastname: N'Dilimabaka
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon.|Institut de Recherche pour le Développement (IRD), Maladies Infectieuses et vecteurs: Ecologie, génétique, Evolution et Contrôle (IRD 224 - CNRS 5290 6 UM1- UM2), Montpellier, France.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Leroy
authors.affiliation: French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Maisons-Alfort, France.
authors.forename: Stéphan
authors.initials: S
authors.lastname: Zientara
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), BP769, Franceville, Gabon.|Centre National de la Recherche Scientifique (CNRS), UMR3569, 25 rue du docteur Roux, 75724, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Berthet
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004474:Ecthyma, Contagious / Q000821:virology*; D019143:Evolution, Molecular; D005681:Gabon; D014644:Genetic Variation; D006041:Goats / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009923:Orf virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017385:Sequence Homology; D012756:Sheep / Q000821:virology*
keywords: Goats; High-throughput sequencing; Orf virus; PCR