title: Ultraviolet B irradiation in the prevention of alloimmunization in a dog platelet transfusion model.
abstract: Alloimmune platelet (PLT) refractoriness remains a significant problem for chronically transfused patients with thrombocytopenia.
  
  
  
  In a dog PLT transfusion model, we evaluated ultraviolet B irradiation (UV-B) of donor PLTs-either alone or in combination with centrifuge leukoreduction (C-LR) or filtration leukoreduction (F-LR)-to prevent refractoriness to donor PLTs and to induce tolerance to standard (STD) PLTs from the same donor or to tertiary donors.
  
  
  
  Recipient acceptance rates for C-LR donor PLT transfusions were 14%, F-LR were 33%, and UV-B irradiated were 45% with no significant differences among the treatments given to the donor's PLTs. Adding UV-B irradiation to C-LR or F-LR PLTs increased acceptance rates to 50 and 68% (p = 0.02 and p = 0.05), respectively, comparing single treatments to the combined treatments. After a recipient had accepted any type of UV-B-treated donor PLTs, specific tolerance to subsequent transfusions of the same donor's STD PLTs averaged 65%. Nonspecific tolerance to third-party donor's STD PLTs averaged 36% if they had accepted their initial donor's treated PLTs but was only 4% (p < 0.001) if they had rejected these PLTs.
  
  
  
  Combining UV-B irradiation with a method of leukoreduction produces additive effects on prevention of alloimmune PLT refractoriness.
authors.affiliation: Research Institute, Puget Sound Blood Center.|University of Washington School of Medicine.
authors.forename: Sherrill J
authors.initials: SJ
authors.lastname: Slichter
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington.
authors.forename: Kraig
authors.initials: K
authors.lastname: Abrams
authors.affiliation: Research Institute, Puget Sound Blood Center.
authors.forename: Irena
authors.initials: I
authors.lastname: Gettinger
authors.affiliation: Research Institute, Puget Sound Blood Center.
authors.forename: Todd
authors.initials: T
authors.lastname: Christoffel
authors.affiliation: Ascendant Laboratories and the Washington Center for Pain Management, Bellevue, Washington.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Gaur
authors.affiliation: Etubics Corporation, Seattle, Washington.
authors.forename: Yvette
authors.initials: Y
authors.lastname: Latchman
authors.affiliation: Research Institute, Puget Sound Blood Center.
authors.forename: Karen
authors.initials: K
authors.lastname: Nelson
authors.affiliation: Research Institute, Puget Sound Blood Center.
authors.forename: Esther
authors.initials: E
authors.lastname: Pellham
authors.affiliation: Research Institute, Puget Sound Blood Center.
authors.forename: S Lawrence
authors.initials: SL
authors.lastname: Bailey
authors.affiliation: Research Institute, Puget Sound Blood Center.
authors.forename: Doug
authors.initials: D
authors.lastname: Bolgiano
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000276:immunology; D004285:Dogs; D007108:Immune Tolerance / Q000528:radiation effects; D007518:Isoantibodies / Q000276:immunology*; D047589:Leukocyte Reduction Procedures; D023421:Models, Animal; D017713:Platelet Transfusion / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D007518:Isoantibodies