title: Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells.
abstract: In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: De Groote
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Sasker
authors.initials: S
authors.lastname: Grootjans
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Saskia
authors.initials: S
authors.lastname: Lippens
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Chantal
authors.initials: C
authors.lastname: Eichperger
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Leurs
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Irene
authors.initials: I
authors.lastname: Kahr
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Giel
authors.initials: G
authors.lastname: Tanghe
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Bruggeman
authors.affiliation: Department of Biomedical Molecular Biology, BCCM/LMBP Plasmid Collection, Ghent University, Belgium.
authors.forename: Wouter
authors.initials: W
authors.lastname: De Schamphelaire
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Corinne
authors.initials: C
authors.lastname: Urwyler
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenabeele
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Jurgen
authors.initials: J
authors.lastname: Haustraete
authors.affiliation: Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.|Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Declercq
mesh_terms: D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D014161:Transduction, Genetic; D019076:Transgenes / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins
keywords: Gateway; Tet-On; co-inducible expression; lentiviral vectors