title: Primary structure, conformation in aqueous solution, and intestinal immunomodulating activity of fucoidan from two brown seaweed species Sargassum crassifolium and Padina australis.
abstract: We studied the structure of fucoidans extracted from two brown seaweed species, Sargassum crassifolium and Padina australis, and their intestinal immunomodulating activity via Peyer's patch cells of C3H/HeJ mice. ESI-MS analysis indicated that the dominant structure of both fucoidans has a backbone of α-(1→4)-linked and α-(1→3)-linked l-fucose residues and sulfate groups are attached at the C-2 and C-4 positions; branches of fucoidan from S. crassifolium are galactose residues with (1→4)- linkage and branching points are at C-4 of fucose, while fucoidan from P. australis, branches are sulfated galactose-fucose disaccharides and sulfated galactose monosaccharides attached to the main chain through (1→3)- or (1→4)- linkages. According to small angle X-ray scattering (SAXS) measurements, the two fucoidans have a branched structure. We simulated them with molecular models based on our proposed primary structure. These fucoidan samples have the ability to stimulate intestinal immunological activity via Peyer's patch cells.
authors.affiliation: Faculty of Engineering, Osaka Electro-Communication University, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yuguchi
authors.affiliation: Nha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Vietnam.
authors.forename: Van Thi Thanh
authors.initials: VTT
authors.lastname: Tran
authors.affiliation: Nha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Vietnam.
authors.forename: Ly Minh
authors.initials: LM
authors.lastname: Bui
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Takebe
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Faculty of Engineering, Osaka Electro-Communication University, Japan.
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Nakajima
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kitamura
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, Vietnam. Electronic address: thuyttt@ich.vast.vn.
authors.forename: Thuy Thi Thu
authors.initials: TTT
authors.lastname: Thanh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D010581:Peyer's Patches / Q000166:cytology / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry; D053838:Scattering, Small Angle; D012624:Seaweed / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides; D014867:Water; C007789:fucoidan
keywords: Fucoidan; Intestinal immunomodulating activity; Padina australis; Sargassum crassifolium; Small angle X-ray scattering