title: Isobolographic Analysis of the Interaction Between Tapentadol and Ketorolac in a Mouse Model of Visceral Pain.
abstract: Preclinical Research The aim of this experimental assay was to assess the antinociceptive interaction between tapentadol and ketorolac in the acetic acid-induced writhing model in mice. Tapentadol (5.62-31.6 mg/kg ip) or ketorolac (5.62-31.6 mg/kg ip) were administered 15 min before the acetic acid administration. The ED50 values of the individual drugs were determined and different proportions (tapentadol-ketorolac in 1:1, 3:1, and 1:3) were assayed in combination in the writhing test. Isobolographic analysis and the interaction index demonstrated an antinociceptive synergistic interaction between tapentadol and ketorolac in all combination. Thus, the experimental ED50 values were lower when compared with their theoretical ED50 values. These data suggest that the tapentadol-ketorolac combination produces an antinociceptive synergistic interaction in the mouse acetic acid-induced writhing model. Drug Dev Res 77 : 187-191, 2016.   © 2016 Wiley Periodicals, Inc.
authors.affiliation: Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, México.
authors.forename: Juan R
authors.initials: JR
authors.lastname: Zapata-Morales
authors.affiliation: Facultad de Medicina, Departamento de Farmacología, Universidad Autónoma de San Luis Potosí, S.L.P., México.
authors.forename: Othoniel H
authors.initials: OH
authors.lastname: Aragon-Martinez
authors.affiliation: Facultad de Odontología, Departamento de Farmacología, Universidad Autónoma de Baja California, campus Mexicali, Mexicali, B.C., México.
authors.forename: Tely
authors.initials: T
authors.lastname: Adriana Soto-Castro
authors.affiliation: Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, México.
authors.forename: Ángel J
authors.initials: ÁJ
authors.lastname: Alonso-Castro
authors.affiliation: Departamento de Investigación, Centro de Educación Avanzada, Tepic, Nayarit, México.
authors.forename: Demian I
authors.initials: DI
authors.lastname: Castañeda-Santana
authors.affiliation: Facultad de Odontología, Departamento de Farmacología, Universidad Autónoma de Baja California, campus Mexicali, Mexicali, B.C., México.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Isiordia-Espinoza
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D020910:Ketorolac / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D000077432:Tapentadol; D059265:Visceral Pain / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000701:Analgesics, Opioid; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010636:Phenols; D000077432:Tapentadol; D019342:Acetic Acid; D020910:Ketorolac
keywords: acute visceral pain test; isobolographic analysis; ketorolac; tapentadol