title: New lipid-dependent Malassezia species from parrots.
abstract: BACKGROUND
  All the currently recognized Malassezia species have been isolated from mammals. However, only a few of them have been isolated from birds. In fact, birds have been less frequently studied as carriers of Malassezia yeasts than mammals.
  
  
  OBJECTIVE
  In this study we describe two new taxa, Malassezia brasiliensis sp. nov. and Malassezia psittaci sp. nov.
  
  
  METHODS
  The isolates studied in this publication were isolated from pet parrots from Brazil. They were characterized using the current morphological and physiological identification scheme. DNA sequencing and analysis of the D1/D2 regions of the 26S rRNA gene, the ITS-5.8S rRNA gene sequences and the β-tubulin gene were also performed.
  
  
  RESULTS
  The strains proposed as new species did not completely fit the phenotypic profiles of any the described species. The validation of these new species was supported by analysis of the genes studied. The multilocus sequence analysis of the three loci provides robust support to delineate these species.
  
  
  CONCLUSIONS
  These studies confirm the separation of these two new species from the other species of the genus Malassezia, as well as the presence of lipid-dependent Malassezia yeasts on parrots.
authors.affiliation: Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain. Electronic address: javier.cabanes@uab.es.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Cabañes
authors.affiliation: Laboratory of Molecular and Cellular Biology, Veterinary Medicine, Paulista University, São Paulo, Brazil.
authors.forename: S Dall' Acqua
authors.initials: SD
authors.lastname: Coutinho
authors.affiliation: Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Puig
authors.affiliation: Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: M Rosa
authors.initials: MR
authors.lastname: Bragulat
authors.affiliation: Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Castellá
mesh_terms: D000818:Animals; D001502:Beak / Q000382:microbiology; D001938:Brazil; D003470:Culture Media / Q000494:pharmacology; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008055:Lipids / Q000494:pharmacology*; D008290:Malassezia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010311:Parrots / Q000382:microbiology*; D010802:Phylogeny; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014404:Tubulin / Q000235:genetics
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D008055:Lipids; D014404:Tubulin
keywords: Aves; Birds; Especiación; Loros; Malassezia brasiliensis; Malassezia psittaci; Parrots; Speciation