title: Anti-tumor and immunomodulatory activities of an exopolysaccharide from Rhizopus nigricans on CT26 tumor-bearing mice.
abstract: This study was aimed to investigate the anti-tumor and immunomodulatory activities of an exopolysaccharide (EPS) from Rhizopus nigricans. Our results showed EPS could significantly inhibit the tumor growth and increase the immune organs index of CT26 tumor-bearing mice. EPS treatment increased the productions of interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) levels in serum. The increase of percentage of CD8(+) cytotoxic T cells among total spleen T lymphocyte was also observed. Furthermore, EPS remarkably stimulate spleen lymphocytes proliferation in the absence or presence of mitogens. In addition, we found that EPS had synergistic effect with chemotherapy and improved immunosuppressive effect induced by 5-Fu. In summary, these findings indicated that the antitumor effects of EPS might be partly due to immune function activation and it might have potential to be used in the treatment for colorectal cancer.
authors.affiliation: Department of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Chemistry and life Science, Guizhou Normal College, Guiyang 550018, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
authors.forename: Guochuang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Yanghui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Department of Pharmacy, Wannan Medical College, Wuhu 241000, China; Anhui Province Key Laboratory of active biological macro-molecules, Wannan Medical College, Wuhu 241000, China; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wannan Medical College, Wuhu 241000, China; School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China. Electronic address: ksc313@126.com.
authors.forename: Kaoshan
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000276:immunology / Q000628:therapy*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007376:Interleukin-2 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011135:Polysaccharides, Bacterial / Q000627:therapeutic use*; D012233:Rhizopus / Q000276:immunology*; D047368:Tumor Burden / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D007166:Immunosuppressive Agents; D007376:Interleukin-2; D011135:Polysaccharides, Bacterial; D014409:Tumor Necrosis Factor-alpha; D005472:Fluorouracil
keywords: Antitumor activity; Exopolysaccharide; Immunomodulatory; Rhizopus nigricans