title: Viscoelastic lithography for fabricating self-organizing soft micro-honeycomb structures with ultra-high aspect ratios.
abstract: High-aspect ratio micro- and nano-structures have been used for the production of a variety of applications. In this paper, we describe a simple and cost-effective approach to fabricate an arrayed microarchitecture with an ultra-high aspect ratio using soft materials. The shapes and sizes of the honeycomb structure can be easily modulated by changing the dimensions and position of the base mould pattern and the pressure. The honeycomb structure is used to prepare a drug delivery patch and a microwell array to form cell spheroids without cell loss. The honeycomb structures prepared using natural ECM (collagen-Matrigel) materials are successfully fabricated. The hepatocytes and endothelial cells are seeded and co-cultured in the ECM-based micro-honeycomb to prepare a 3D liver model successfully mimicking an ultrastructure of liver and providing enhanced liver function.
authors.affiliation: Department of Biomedical Engineering, College of Health Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, Korea.|Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 olympic-Ro, Songpa-gu, Seoul 05505, Korea.
authors.forename: Gi Seok
authors.initials: GS
authors.lastname: Jeong
authors.affiliation: Department of Biomedical Engineering, College of Health Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, Korea.|Department of Bioengineering, School of Engineering and Medicine, Stanford University, 443 Via Ortega, Stanford, California 94305, USA.
authors.forename: Da Yoon
authors.initials: da Y
authors.lastname: No
authors.affiliation: KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Korea.
authors.forename: JaeSeo
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Mechanical Engineering, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, Korea.
authors.forename: Junghyo
authors.initials: J
authors.lastname: Yoon
authors.affiliation: School of Mechanical Engineering, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, Korea.
authors.forename: Seok
authors.initials: S
authors.lastname: Chung
authors.affiliation: Department of Biomedical Engineering, College of Health Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, Korea.|KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Korea.
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee