title: Chimeric foot-and-mouth disease virus serotype O displaying a serotype Asia1 antigenic epitope at the surface.
abstract: OBJECTIVE
  To determine whether the G-H loop of foot-and-mouth disease virus (FMDV) serotype O can function as a target structure to harbour and display serotype Asia1 antigenic epitope at the surface.
  
  
  RESULTS
  Using reverse genetics, FMDV serotype O IND R2/1975 displaying a FMDV serotype Asia1 B cell epitope at the capsid surface was constructed. The epitope-inserted recombinant chimeric virus was genetically stable up to ten serial passages in cell culture and exhibited growth properties similar to the parental serotype O virus. Furthermore, the surface-displayed Asia1 epitope able to react with serotype Asia1 specific antibodies in a competitive ELISA. Importantly, the recombinant chimeric virus showed neutralizing activity to both serotype O and Asia1 polyclonal antibodies.
  
  
  CONCLUSIONS
  The capsid protein of FMDV serotype O can effectively display potent epitope of other serotypes, making this an attractive approach for the design of new generation bi-valent FMD vaccines.
authors.affiliation: ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteswar, Nainital, Uttarakhand, 263138, India. jkubiswal@gmail.com.
authors.forename: Jitendra K
authors.initials: JK
authors.lastname: Biswal
authors.affiliation: ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteswar, Nainital, Uttarakhand, 263138, India.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Ranjan
authors.affiliation: ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteswar, Nainital, Uttarakhand, 263138, India.
authors.forename: Bramhadev
authors.initials: B
authors.lastname: Pattnaik
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D065288:Serogroup
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes
keywords: Asia1 epitope; Chimeric foot-and-mouth disease virus; Epitope insertion; Foot-and-mouth disease virus; Reverse genetics