title: Carbonic anhydrase IX deposits are associated with increased ascending aortic dilatation.
abstract: OBJECTIVE
  Carbonic anhydrase IX (CA IX) expression is induced by local hypoxia. We studied whether CA IX deposits associate with ascending aortic dilatation.
  
  
  METHODS
  Aortic wall histology, CA IX expression, presence of leukocytes, plasma cells, macrophages, endothelial cells, smooth muscle cells, cell proliferation, elastin and collagen were studied in histological specimens collected from 30 patients who underwent surgery for ascending aorta. The samples were grouped according to presence of CA IX deposits.
  
  
  RESULTS
  Twenty out of 30 patients had CA IX-positive deposits within the adventitia, whereas 10 specimens remained negative. Adventitial inflammation was increased in CA IX-positive samples as compared with CA IX-negative ones (p < 0.01). The mean diameter of the ascending aorta at the sinotubular junction increased significantly in patients with CA IX-positive staining as compared with CA IX-negative cases (63 ± 3 vs 53 ± 2 mm, p < 0.02). Receiver operating characteristic curve analysis confirmed the association of CA IX positivity with increased ascending aortic dilatation (AUC 0.766; S.E. 0.090; p = 0.020; 95% C.I. 0.590-0.941).
  
  
  CONCLUSIONS
  Positive CA IX staining in certain aortic specimens suggests that increased CA activity may contribute to ascending aortic dilatation.
authors.affiliation: b School of Medicine , University of Tampere , Tampere , Finland ;
authors.forename: Eetu
authors.initials: E
authors.lastname: Niinimaki
authors.affiliation: b School of Medicine , University of Tampere , Tampere , Finland ;
authors.forename: Petteri
authors.initials: P
authors.lastname: Muola
authors.affiliation: b School of Medicine , University of Tampere , Tampere , Finland ;|c Department of Pathology, Fimlab Laboratories, Tampere University Hospital and Tampere University Medical School , Tampere , Finland ;
authors.forename: Seppo
authors.initials: S
authors.lastname: Parkkila
authors.affiliation: c Department of Pathology, Fimlab Laboratories, Tampere University Hospital and Tampere University Medical School , Tampere , Finland ;
authors.forename: Ivana
authors.initials: I
authors.lastname: Kholová
authors.affiliation: c Department of Pathology, Fimlab Laboratories, Tampere University Hospital and Tampere University Medical School , Tampere , Finland ;
authors.forename: Hannu
authors.initials: H
authors.lastname: Haapasalo
authors.affiliation: d Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences , Bratislava , Slovak Republic ;
authors.forename: Silvia
authors.initials: S
authors.lastname: Pastorekova
authors.affiliation: d Department of Molecular Medicine, Institute of Virology , Slovak Academy of Sciences , Bratislava , Slovak Republic ;
authors.forename: Jaromir
authors.initials: J
authors.lastname: Pastorek
authors.affiliation: c Department of Pathology, Fimlab Laboratories, Tampere University Hospital and Tampere University Medical School , Tampere , Finland ;
authors.forename: Timo
authors.initials: T
authors.lastname: Paavonen
authors.affiliation: a Heart Center , Tampere University Hospital , Tampere , Finland ;|e Heart Center , Turku University Hospital , Turku , Finland.
authors.forename: Ari
authors.initials: A
authors.lastname: Mennander
mesh_terms: D000368:Aged; D001011:Aorta / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D001018:Aortic Diseases / Q000201:enzymology / Q000209:etiology / Q000473:pathology; D000071231:Carbonic Anhydrase IX / Q000032:analysis / Q000378:metabolism; D004108:Dilatation, Pathologic / Q000201:enzymology / Q000209:etiology / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D011897:Random Allocation; D013223:Statistics as Topic; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D000071231:Carbonic Anhydrase IX
keywords: Ascending aortic dilatation; CA IX; inflammation