title: A simple improved desolvation method for the rapid preparation of albumin nanoparticles.
abstract: The current study tried to establish a simple and fast method for the preparation of BSA and HSA nanoparticles, based on an improved desolvation procedure under the aspect of a controllable particle size around 100nm for drug delivery applications. The Procedure used for the nanoparticles preparation was simplified by using a designed apparatus for controlling the addition of ethanol and it was used instead of conventional tubing pump which enabled the preparation of nanoparticles under defined conditions. By using EDC as cross-linker instead of glutharaldehyde, the time of nanoparticles preparation procedure was reduced to 3h. Several factors of the preparation process, such as the volume of the albumin solution, desolvating agent volume, the amount of cross-linker, the presence of salts and protein concentration were evaluated. Nanoparticles with smaller size were obtained under experimental conditions without the presence of salts or the use of buffers, 250mg of protein/4ml water, 5mg cross-linker, the addition of 4 and 8ml ethanol by using the designed apparatus to the HSA and BSA solution, respectively. By using this improved method, BSA and HSA nanoparticles of the size around 100nm and polydispersity below 0.2 were obtained.
authors.affiliation: Biotechnology Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran; School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jahanban-Esfahlan
authors.affiliation: Biotechnology Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran; School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Siavoush
authors.initials: S
authors.lastname: Dastmalchi
authors.affiliation: School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: davaran@tbzmed.ac.ir.
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Davaran
mesh_terms: D000818:Animals; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012492:Salts / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000648:ultrastructure; D012996:Solutions
chemical_list: D003432:Cross-Linking Reagents; D012492:Salts; D012709:Serum Albumin; D012996:Solutions; D012710:Serum Albumin, Bovine; D000431:Ethanol
keywords: Desolvation technique; Drug delivery; Protein