title: Relationship of the rs1799752 polymorphism of the angiotensin-converting enzyme gene and the rs699 polymorphism of the angiotensinogen gene to the process of in-stent restenosis in a population of Polish patients with stable coronary artery disease.
abstract: OBJECTIVE
  The renin-angiotensin-aldosterone system may influence in-stent restenosis (ISR) via angiotensin II, which stimulates the production of growth factors for smooth muscle cells. The aim of this work is to assess the influence of the rs1799752 polymorphism of the angiotensin-converting enzyme (ACE) gene and the rs699 polymorphism of the angiotensinogen (AGT) gene on the ISR in Polish patients with stable coronary artery disease (SCAD) who underwent stent implantation.
  
  
  METHODS
  Two hundred and sixty-five patients with SCAD were included in the study. All patients underwent stent implantation upon admission to the hospital and had subsequent coronary angiography performed. The patients were divided into two groups - those with significant ISR (n=53) and those without ISR (n=212). The ACE polymorphism was assessed using the classical PCR method and the AGT polymorphism was determined using the TaqMan method for SNP genotyping.
  
  
  RESULTS
  No difference in the frequency of angiographically significant ISR occurrence associated with the different ACE and AGT gene polymorphisms was observed. In a multivariable analysis, after correction for clinical variables, the relationship between the ACE and AGT genotypes within the scope of the analyzed polymorphisms and the process of restenosis was not found using a dominant, recessive and log-additive model. Late lumen loss was also independent of the genotypes of the polymorphisms before and after correction with angiographic variables.
  
  
  CONCLUSIONS
  The rs1799752 polymorphism and the rs699 polymorphism had no relationship with the occurrence of angiographically significant ISR and late lumen loss in a group of Polish patients who underwent metal stent implantation.
authors.affiliation: 2nd Department of Cardiology and Angiology, Silesian Center for Heart Diseases, Zabrze, Poland; Genomics Laboratory, Kardio-Med Silesia Science and Technology Park, Zabrze, Poland. Electronic address: tadeusz.osadnik@sccs.pl.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Osadnik
authors.affiliation: Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Joanna Katarzyna
authors.initials: JK
authors.lastname: Strzelczyk
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland; Genomics Laboratory, Kardio-Med Silesia Science and Technology Park, Zabrze, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Fronczek
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Bujak
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Reguła
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Gonera
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Gawlita
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kurek
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Wasilewski
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Lekston
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Gierlotka
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Hawranek
authors.affiliation: Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Ostrowska
authors.affiliation: Department of Medical and Molecular Biology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wiczkowski
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Lech
authors.initials: L
authors.lastname: Poloński
authors.affiliation: 3rd Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gąsior
mesh_terms: D000368:Aged; D000808:Angiotensinogen / Q000235:genetics*; D015331:Cohort Studies; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000150:complications / Q000201:enzymology* / Q000235:genetics*; D023903:Coronary Restenosis / Q000150:complications / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011044:Poland; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015607:Stents
chemical_list: D000808:Angiotensinogen; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A
keywords: Angiotensin-converting enzyme; Angiotensinogen; In-stent restenosis; Polymorphism