title: Electron Paramagnetic Resonance of a Single NV Nanodiamond Attached to an Individual Biomolecule.
abstract: Electron paramagnetic resonance (EPR), an established and powerful methodology for studying atomic-scale biomolecular structure and dynamics, typically requires in excess of 10(12) labeled biomolecules. Single-molecule measurements provide improved insights into heterogeneous behaviors that can be masked in ensemble measurements and are often essential for illuminating the molecular mechanisms behind the function of a biomolecule. Here, we report EPR measurements of a single labeled biomolecule. We selectively label an individual double-stranded DNA molecule with a single nanodiamond containing nitrogen-vacancy centers, and optically detect the paramagnetic resonance of nitrogen-vacancy spins in the nanodiamond probe. Analysis of the spectrum reveals that the nanodiamond probe has complete rotational freedom and that the characteristic timescale for reorientation of the nanodiamond probe is slow compared with the transverse spin relaxation time. This demonstration of EPR spectroscopy of a single nanodiamond-labeled DNA provides the foundation for the development of single-molecule magnetic resonance studies of complex biomolecular systems.
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Richelle M
authors.initials: RM
authors.lastname: Teeling-Smith
authors.affiliation: Samsung Electronics, San #24 Nongseo-Dong, Giheung-Gu, Yongin-City, Gyonggi-Do, Korea.
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Jung
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Scozzaro
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Cardellino
authors.affiliation: Columbus Nanoworks, Columbus, Ohio.
authors.forename: Isaac
authors.initials: I
authors.lastname: Rampersaud
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Justin A
authors.initials: JA
authors.lastname: North
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Marek
authors.initials: M
authors.lastname: Šimon
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Vidya P
authors.initials: VP
authors.lastname: Bhallamudi
authors.affiliation: Columbus Nanoworks, Columbus, Ohio.
authors.forename: Arfaan
authors.initials: A
authors.lastname: Rampersaud
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Ezekiel
authors.initials: E
authors.lastname: Johnston-Halperin
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Poirier
authors.affiliation: Department of Physics, The Ohio State University, Columbus, Ohio. Electronic address: hammel@mps.ohio-state.edu.
authors.forename: P Chris
authors.initials: PC
authors.lastname: Hammel
mesh_terms: D004247:DNA / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D058612:Nanodiamonds / Q000737:chemistry*
chemical_list: D058612:Nanodiamonds; D004247:DNA