title: Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan.
abstract: A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated as NCCP-1340(T), was isolated from a hot water spring, Tatta Pani, Pakistan. Cells of strain NCCP-1340(T) were observed to be aerobic, rod shaped, catalase and urease positive but H2S production and oxidase negative. Growth was observed at pH 6.0-8.0 (optimum pH 7.0) and at 20-40 °C (optimum 37 °C). The strain could tolerate 0-8 % NaCl (optimum 2 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NCCP-1340(T) belongs to the genus Nocardioides and is closely related to Nocardioides iriomotensis JCM 17985(T) (96.8 %), Nocardioides daedukensis KCTC 19601(T) (96.6 %), Nocardioides jensenii KCTC 9134(T) (96.1 %) and Nocardioides daejeonensis KCTC 19772(T) (96.1 %). The DNA-DNA relatedness values of strain NCCP-1340(T) with N. iriomotensis JCM 17985(T), N. daedukensis KCTC 19601(T) and N. jensenii KCTC 9134(T) were found to be less than 53 %. The DNA G+C content of strain NCCP-1340(T) was determined to be 71.8 mol  %. The affiliation of strain NCCP-1340(T) to the genus Nocardioides was further supported by chemotaxonomic data which showed the presence of MK-8(H4) as major menaquinone system; iso-C16:0, C17:0, C16:0 10-methyl, iso-C15:0 and C 15:0 as major cellular fatty acids; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and unidentified glycolipids and polar lipids in the polar lipids profile. The cell wall peptidoglycan contained LL-diaminopimelic acid as the diagnostic amino acid. On the basis of physiological and biochemical characteristics and the phylogenetic analyses, strain NCCP-1340(T) can be distinguished from the closely related taxa and thus represents a novel species of the genus Nocardioides, for which the name Nocardioides pakistanensis sp. nov. is proposed with the type strain NCCP-1340(T) (= DSM 29942(T) = JCM 30630(T)).
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China.|Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan.|Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Arshia
authors.initials: A
authors.lastname: Amin
authors.affiliation: Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan. iftikharnarc@hotmail.com.|PARC Institute of Advance Studies in Agriculture (PIASA), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan. iftikharnarc@hotmail.com.
authors.forename: Iftikhar
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China.
authors.forename: Neeli
authors.initials: N
authors.lastname: Habib
authors.affiliation: Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan.|PARC Institute of Advance Studies in Agriculture (PIASA), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan.
authors.forename: Saira
authors.initials: S
authors.lastname: Abbas
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Bioproducts Research Chair (BRC) College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010154:Pakistan; D010641:Phenotype; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D012965:Sodium Chloride; D003960:Diaminopimelic Acid
keywords: Hot water spring; Nocardioides pakistanensis sp. nov.; Tatta Pani Pakistan