title: Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron.
abstract: In the Drosophila olfactory system, odorant information is sensed by olfactory sensory neurons and relayed from the primary olfactory center, the antennal lobe (AL), to higher olfactory centers via olfactory projection neurons (PNs). A major portion of the AL is constituted with dendrites of four groups of PNs, anterodorsal PNs (adPNs), lateral PNs (lPNs), lateroventral PNs (lvPNs) and ventral PNs (vPNs). Previous studies have been focused on the development and function of adPNs and lPNs, while the investigation on those of lvPNs and vPNs received less attention. Here, we study the molecular and cellular mechanisms underlying the morphogenesis of a putative male-pheromone responding vPN, the DA1 vPN. Using an intersection strategy to remove background neurons labeled within a DA1 vPN-containing GAL4 line, we depicted morphological changes of the DA1 vPN that occurs at the pupal stage. We then conducted a pilot screen using RNA interference knock-down approach to identify cell surface molecules, including Down syndrome cell adhesion molecule 1 and Semaphorin-1a, that might play essential roles for the DA1 vPN morphogenesis. Taken together, by revealing molecular and cellular basis of the DA1 vPN morphogenesis, we should provide insights into future comprehension of how vPNs are assembled into the olfactory neural circuitry.
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.|Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Hung-Chang
authors.initials: HC
authors.lastname: Shen
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Wei
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Sao-Yu
authors.initials: SY
authors.lastname: Chu
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Pei-Chi
authors.initials: PC
authors.lastname: Chung
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Tsai-Chi
authors.initials: TC
authors.lastname: Hsu
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.|Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Hung-Hsiang
authors.initials: HH
authors.lastname: Yu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015815:Cell Adhesion Molecules; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008297:Male; D009024:Morphogenesis / Q000235:genetics*; D019006:Neural Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009833:Olfactory Pathways / Q000378:metabolism* / Q000648:ultrastructure; D018034:Olfactory Receptor Neurons / Q000378:metabolism* / Q000648:ultrastructure; D011679:Pupa / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D012724:Sex Attractants / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D012903:Smell / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C491736:Dscam1 protein, Drosophila; C557617:GAL4 protein, Drosophila; D019006:Neural Cell Adhesion Molecules; D034741:RNA, Small Interfering; D039961:Semaphorins; D012724:Sex Attractants; D014157:Transcription Factors; C477531:semaphorin 1a, Drosophila