title: A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro.
abstract: Angiogenesis, an integral part of many physiological and pathological processes, is a tightly regulated multistep process. Angiogenesis assays are used to clarify the molecular mechanisms and screen for pharmacological inhibitors. However, most in vitro angiogenesis models measure only one aspect of this process, whereas in vivo assays are complex and difficult to interpret. The ex vivo aortic ring model allows the study of many key features of angiogenesis, such as endothelial activation, branching, and remodeling as well as later steps such as pericyte acquisition. This model can be modified to include genetic manipulation and can be used to assess the pro- or anti-angiogenic effects of compounds in a relatively controlled system.
authors.affiliation: Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Theodor-Stern-Kai 7, Frankfurt am Main, Frankfurt, D-60590, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Zippel
authors.affiliation: Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Theodor-Stern-Kai 7, Frankfurt am Main, Frankfurt, D-60590, Germany.
authors.forename: Yindi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Institute for Vascular Signalling, Centre for Molecular Medicine, Goethe University, Theodor-Stern-Kai 7, Frankfurt am Main, Frankfurt, D-60590, Germany. fleming@em.uni-frankfurt.de.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Fleming
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology* / Q000648:ultrastructure; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology*; D051379:Mice; D018613:Microscopy, Confocal; D018919:Neovascularization, Physiologic; D051381:Rats; D046509:Tissue Culture Techniques
keywords: Angiogenesis; Endothelium; Mouse aorta; Organ culture; Pericyte recruitment; Sprouting