title: [Expression Patterns of the Proteins Associated with Cell Junctions in Mouse Testes].
abstract: OBJECTIVE
  To study on the expression patterns of proteins associated with cell junctions in the developing mouse testes.
  
  
  METHODS
  The expression levels of reproductive related cell lines spermatogonia cell line GC1 spg, spermatocyte cell line GC2 spg, leydig cell line TM3, and sertoli cell line TM4, primary sertoli cells, and 1-6-week mouse testes were analyzed using Western blot.
  
  
  RESULTS
  The sertoli cell junction-associated membrane proteins adhesion molecule A, Occludin and Claudin, and the sertoli-germ cell junction-associated membrane proteins junctional adhesion molecule C, Nectin-3, and E-cadherin were stage-specific in the seminiferous tubules in the mouse testes. The adaptor proteins associated with cell juctions zonula occludens-1, zonula occludens-2, Afadin, Β-catenin, and CD2-associated protein were not stage-specific in the seminiferous tubules in the mouse testes.
  
  
  CONCLUSIONS
  In the seminiferous tubules in the mouse testes, the membrane proteins associated with cell junctions are stage-specific. However, the expressions of adaptor proteins associated with cell junctions do not obviously change.
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College,Beijing 100005,China;
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College,Beijing 100005,China;
authors.forename: Yan-yun
authors.initials: YY
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College,Beijing 100005,China;
authors.forename: Shi-ying
authors.initials: SY
authors.lastname: Miao
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College,Beijing 100005,China;
authors.forename: Lin-fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D064200:Cdh1 Proteins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism; D000075983:Nectins; D012671:Seminiferous Tubules / Q000166:cytology* / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology; D013737:Testis / Q000166:cytology*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*; D062827:Zonula Occludens-2 Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C114545:CD2-associated protein; C495267:CTNNB1 protein, mouse; D064200:Cdh1 Proteins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C577585:Fzr1 protein, mouse; D008565:Membrane Proteins; D008840:Microfilament Proteins; C000619014:NECTIN3 protein, human; C000619017:Nectin3 protein, mouse; D000075983:Nectins; C569043:Tjp1 protein, mouse; C569047:Tjp2 protein, mouse; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; C108835:afadin; D051176:beta Catenin