title: Expression of Estrogen Receptors Alpha (ER-α), Beta (ER-β), and G Protein-Coupled Receptor 30 (GPR30) in Testicular Tissue of Men with Klinefelter Syndrome.
abstract: Men with Klinefelter syndrome (KS) present severe hormonal dysregulation, particularly elevated serum estradiol concentration. Estrogens act through specific receptors and regulate testes development and spermatogenesis. Herein, we evaluated GPR30, ERα, and ERβ mRNA expression in testis of KS men and men with 46XY karyotype by reverse transcriptase and quantitative PCR. ERβ transcripts are the most abundant in testicular tissue of 46XY men. Notably, testicular GPR30 transcription in KS men was approximately 12 times higher. Since GPR30 is essential to mediate estrogen effects over steroidogenesis, our data illustrate that GPR30 may underpin the testicular alterations observed in KS men.
authors.affiliation: Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal.
authors.forename: R L
authors.initials: RL
authors.lastname: Bernardino
authors.affiliation: CICS - UBI - Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
authors.forename: M G
authors.initials: MG
authors.lastname: Alves
authors.affiliation: Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Silva
authors.affiliation: Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Barros
authors.affiliation: Department of Urology, Hospital Center of Vila Nova de Gaia, Vila Nova de Gaia, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Ferraz
authors.affiliation: Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Sousa
authors.affiliation: Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Sá
authors.affiliation: Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal.
authors.forename: P F
authors.initials: PF
authors.lastname: Oliveira
mesh_terms: D000328:Adult; D015992:Body Mass Index; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007713:Klinefelter Syndrome / Q000235:genetics*; D008297:Male; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; C109584:GPER1 protein, human; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled