title: A Sensitive, Colorimetric, High-Throughput Loop-Mediated Isothermal Amplification Assay for the Detection of Plasmodium knowlesi.
abstract: The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.
authors.affiliation: University of Queensland, Brisbane, Australia. QIMR Berghofer Medical Research Institute, Brisbane, Australia. sumudu.britton@qimrberghofer.edu.au.
authors.forename: Sumudu
authors.initials: S
authors.lastname: Britton
authors.affiliation: Australian Army Malaria Institute, Brisbane, Australia.
authors.forename: Qin
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: Menzies School of Health Research, Charles Darwin University, Darwin, Australia.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Grigg
authors.affiliation: Clinical Research Centre, Queen Elizabeth Hospital, Kota Kinabalu, Sabah, Malaysia. Jesselton Medical Center, Kota Kinabalu, Sabah, Malaysia.
authors.forename: Timothy
authors.initials: T
authors.lastname: William
authors.affiliation: Menzies School of Health Research, Charles Darwin University, Darwin, Australia.
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Anstey
authors.affiliation: University of Queensland, Brisbane, Australia. QIMR Berghofer Medical Research Institute, Brisbane, Australia.
authors.forename: James S
authors.initials: JS
authors.lastname: McCarthy
mesh_terms: D000818:Animals; D003124:Colorimetry; D017931:DNA Primers / Q000235:genetics; D003955:Diagnostic Tests, Routine; D000882:Haplorhini / Q000469:parasitology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008288:Malaria / Q000175:diagnosis*; D008296:Malaysia; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016790:Plasmodium knowlesi / Q000235:genetics / Q000302:isolation & purification*; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers