title: Epidermal growth factor loaded heparin-based hydrogel sheet for skin wound healing.
abstract: A heparin-based hydrogel sheet composed of thiolated heparin and diacrylated poly (ethylene glycol) was prepared via photo polymerization and human epidermal growth factor (hEGF) were loaded into it for the purpose of wound healing. It showed a sustained release profile of hEGF in vitro. In order to evaluate its function on wound healing in vivo, full thickness wounds were created on the dorsal surface of mice. Application of hEGF loaded heparin-based hydrogel sheet accelerated the wound closure compared to the non-treated control group, hEGF solution, and hEGF loaded PEG hydrogel sheet. Histological and immunohistological examinations also demonstrated an advanced granulation tissue formation, capillary formation, and epithelialization in wounds treated by hEGF loaded heparin-based hydrogel compared to other groups, and no biocompatibility issue was observed. In conclusion, the delivery of hEGF using the heparin-based hydrogel could accelerate the skin wound healing process.
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: chyn87fish@gist.ac.kr.
authors.forename: MeeiChyn
authors.initials: M
authors.lastname: Goh
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: ymhwang@gist.ac.kr.
authors.forename: Youngmin
authors.initials: Y
authors.lastname: Hwang
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea; Center for theragnosis, Biomedical research institute, KIST, Seoul 02792, Republic of Korea. Electronic address: gytae@gist.ac.kr.
authors.forename: Giyoong
authors.initials: G
authors.lastname: Tae
mesh_terms: D000818:Animals; D066255:EGF Family of Proteins / Q000737:chemistry / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry; D012867:Skin / Q000187:drug effects / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D066255:EGF Family of Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D006493:Heparin
keywords: Epidermal growth factor; Heparin; Hydrogel; Skin; Wound healing