title: Medical ozone treatment ameliorates the acute distal colitis in rat.
abstract: OBJECTIVE
  To investigate the effect of medical ozone treatment on the experimental acute distal colitis in rats.
  
  
  METHODS
  Eighteen rats were randomly distributed into three equal groups; control, acute distal colitis (ADC) without and with medical ozone treatment. Rats in the control group were taken saline. ADC was performed by rectal way with 4% acetic acid in groups 2 and 3, and the group 3 was treated with medical ozone for three weeks both rectally and intraperitoneally. At the twenty second day the distal colons samples were obtained for malondialdehyde and myeloperoxidase, blood samples were obtained to measure the levels of TNF-α and IL-1β levels. Histolopatological examination was evaluated with Ki-67, IL-1β and VEGF immunostaining densities.
  
  
  RESULTS
  There was significant increase in tissue MDA, MPO activity, TNF-α and IL-1β after ozone administration. There was also a significant difference at immunostaining densities of histopathological examination.
  
  
  CONCLUSIONS
  Medical ozone treatment ameliorated the experimental acute distal colitis induced by acetic acid in rats. Its possible effect is by means of decreasing inflammation, edema, and affecting the proliferation and the vascularization.
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Arif
authors.initials: A
authors.lastname: Aslaner
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Tuğrul
authors.initials: T
authors.lastname: Çakır
authors.affiliation: Department of Biochemistry, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Seçkin Özgür
authors.initials: SÖ
authors.lastname: Tekeli
authors.affiliation: Department of Histology and Embryology, Akdeniz University, Antalya, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Avcı
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Doğan
authors.affiliation: Department of Biochemistry, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Feyza
authors.initials: F
authors.lastname: Tekeli
authors.affiliation: Department of Histology and Embryology, Akdeniz University, Antalya, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Soylu
authors.affiliation: Department of Gastroenterology, Haydarpaşa Training and Research Hospital, Istanbul, Turkey.
authors.forename: Cebrail
authors.initials: C
authors.lastname: Akyüz
authors.affiliation: Faculty of Medicine, Inonü University, Malatya, Turkey.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Koç
authors.affiliation: Department of Histology and Embryology, Akdeniz University, Antalya, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Üstünel
authors.affiliation: Department of Biochemistry, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Necat
authors.initials: N
authors.lastname: Yılmaz
mesh_terms: D019342:Acetic Acid; D000208:Acute Disease; D000818:Animals; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000473:pathology; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010083:Oxidants, Photochemical / Q000627:therapeutic use*; D010126:Ozone / Q000627:therapeutic use*; D009195:Peroxidase / Q000032:analysis; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D053583:Interleukin-1beta; D010083:Oxidants, Photochemical; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D008315:Malondialdehyde; D010126:Ozone; D009195:Peroxidase; D019342:Acetic Acid