title: A Phosphate Starvation-Inducible Ribonuclease of Bacillus licheniformis.
abstract: The BLi03719 protein of Bacillus licheniformis DSM13 belongs to the most abundant extracellular proteins under phosphate starvation conditions. In this study, the function of this phosphate starvation inducible protein was determined. An amino-acid sequence analysis of the BLi03719-encoding gene showed a high similarity with genes encoding the barnase of Bacillus amyloliquefaciens FZB42 and binase-like RNase of Bacillus pumilus SARF-032. The comparison of the control strain and a BLi03719-deficient strain revealed a strongly reduced extracellular ribonuclease activity of the mutant. Furthermore, this knockout mutant exhibited delayed growth with yeast RNA as an alternative phosphate and carbon source. These results suggest that BLi03719 is an extracellular ribonuclease expressed in B. licheniformis under phosphate starvation conditions. Finally, a BLi03719 mutant showed an advantageous effect on the overexpression of the heterologous amyE gene under phosphate-limited growth conditions.
authors.affiliation: Center for Molecular Biology, Institute of Research and Development, Duy Tan University, Danang, Vietnam.
authors.forename: Thanh Trung
authors.initials: TT
authors.lastname: Nguyen
authors.affiliation: Center for Molecular Biology, Institute of Research and Development, Duy Tan University, Danang, Vietnam.
authors.forename: Minh Hung
authors.initials: MH
authors.lastname: Nguyen
authors.affiliation: Center for Molecular Biology, Institute of Research and Development, Duy Tan University, Danang, Vietnam.
authors.forename: Huy Thuan
authors.initials: HT
authors.lastname: Nguyen
authors.affiliation: Faculty of Food Science and Technology, Vietnam National University of Agriculture, Hanoi, Vietnam.
authors.forename: Hoang Anh
authors.initials: HA
authors.lastname: Nguyen
authors.affiliation: Clinical Laboratory, National Hospital of Tropical Diseases, Hanoi, Vietnam.
authors.forename: Thi Hoi
authors.initials: TH
authors.lastname: Le
authors.affiliation: Pharmaceutical Biotechnology, Institute of Pharmacy, Ernst-Moritz-Arndt-University, 17489 Greifswald, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schweder
authors.affiliation: Pharmaceutical Biotechnology, Institute of Pharmacy, Ernst-Moritz-Arndt-University, 17489 Greifswald, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Jürgen
mesh_terms: D000595:Amino Acid Sequence; D000069976:Bacillus licheniformis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins; D004722:Endoribonucleases / Q000235:genetics; D005798:Genes, Bacterial; D010710:Phosphates / Q000378:metabolism*; D012260:Ribonucleases / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017384:Sequence Deletion
chemical_list: D001426:Bacterial Proteins; D010710:Phosphates; D004722:Endoribonucleases; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease; C022543:ribonuclease T(2)
keywords: Bacillus licheniformis; barnase; binase; phosphate starvation; ribonuclease