title: Antioxidant effect of Legalon(r) SIL in ischemia-reperfusion injury of rat skeletal muscle.
abstract: OBJECTIVE
  To evaluated the potential antioxidant agent Legalon (r) SIL (silibinin-C-2',3-bis(hydrogensuccinat)) in the skeletal muscle of rats.
  
  
  METHODS
  IRI was achieved via tourniquet application in Wistar-albino rats. Experimental groups were chosen as (i) sham control, (ii) IRI (3+2 h), (iii) IRI and Legalon (r) SIL-50 (50 mg/kg/i.p.), (iv) IRI and Legalon (r) SIL-100 (100 mg/kg/i.p.), and (v) IRI and Legalon (r) SIL-200 (200 mg/kg/ i.p.). Muscle viability (evaluated by triphenyltetrazolium chloride dye method), malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase were assessed in muscle samples using a spectrophotometer.
  
  
  RESULTS
  Although viability of the injured limb non-significantly declined in the IRI group, administration of Legalon (r) SIL did not prevent injury. However, dramatic increase observed in malondialdehyde levels in the IRI group was prohibited by Legalon (r) SIL in a statistically significant manner. In comparison with the sham-control group, IRI and Legalon (r) SIL administration did not cause any significant alterations in the levels of superoxide dismutase, catalase, and glutathione peroxidase.
  
  
  CONCLUSIONS
  Although Legalon (r) SIL was not sufficient to prevent muscle injury in terms of viability, it is found to be an effective option to reduce reactive oxygen species-induced cell injury.
authors.affiliation: School of Medicine, Kahramanmaraş Sütçü İmam University, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Ergün
authors.affiliation: School of Medicine, Kahramanmaraş Sütçü İmam University, Turkey.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Üremiş
authors.affiliation: School of Medicine, Kahramanmaraş Sütçü İmam University, Turkey.
authors.forename: Metin
authors.initials: M
authors.lastname: Kılınç
authors.affiliation: Department of Traumatology & Orthopedics, Üsküdar State Hospital, İstanbul, Turkey.
authors.forename: Tuğrul
authors.initials: T
authors.lastname: Alıcı
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000032:analysis / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000032:analysis / Q000187:drug effects; D007511:Ischemia / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000737:chemistry / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000032:analysis; D012016:Reference Values; D015427:Reperfusion Injury / Q000517:prevention & control*; D015203:Reproducibility of Results; D012838:Silymarin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000032:analysis / Q000187:drug effects; D014022:Tissue Survival / Q000187:drug effects
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D012838:Silymarin; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase