title: CYP1A2-163C/A (rs762551) polymorphism and bladder cancer risk: a case-control study.
abstract: To date, no study has investigated the association between CYP1A2-163C/A polymorphism and bladder cancer risk in a Chinese population. Here, we extracted genomic DNA from peripheral white blood cells, and differentiated CYP1A2 alleles by polymerase chain reaction-based restriction fragment length polymorphism methods. Differences in genotype frequencies between the cases and controls were evaluated using a chi-square test. The odds ratio (OR) and its 95% confidence interval (CI) were calculated using an unconditional logistic regression model. This revealed that the -163A allele was present at a significantly increased frequency in bladder cancer patients compared to healthy controls (44.10 vs 22.25%, P < 0.001). The prevalence of CC genotype, CA genotype, and AA genotype was 34.91, 41.98, and 23.11% in bladder cancer patients, and 64.00, 27.50, and 8.5% in the controls, respectively. Therefore, significant differences in the frequencies of -163 genotypes were found between bladder cancer patients and controls (P < 0.001). We found that the AA genotype was significantly associated with increased bladder cancer risk (OR = 3.72; 95%CI = 1.55-7.16; P = 0.02), and the -163A carriers were at increased risk of bladder cancer in a multivariate COX regression model (OR = 4.89, 95%CI = 2.78-10.87, P = 0.01). We conclude that the CYP1A2-163C/A polymorphism is associated with increased susceptibility to bladder cancer in the Chinese population.
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, China.|Department of Urology, Zaozhuang Municipal Hospital, Zaozhuang, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Song
authors.affiliation: Department of Urology, Zaozhuang Municipal Hospital, Zaozhuang, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: J C
authors.initials: JC
authors.lastname: Ren
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Xu
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: C504573:CYP1A2 protein, human; D019388:Cytochrome P-450 CYP1A2