title: Isolation and Transfection of Primary Culture Bovine Retinal Pericytes.
abstract: This protocol describes an enzymatic approach for isolating homogeneous cultures of pericytes from retinas of bovine source. In summary, retinas are dissected, washed, digested, filtered, cultured in specific media to select for pericytes, and finally expanded for a low passage culture of about 14 million bovine retinal pericytes (BRP) within 4-6 weeks. This protocol also describes a liposomal-based technique for transfection of BRPs.
authors.affiliation: Department of Ophthalmology, Schepens Eye Research Institute, Mass Eye and Ear Infirmary, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA.
authors.forename: Vincent A
authors.initials: VA
authors.lastname: Primo
authors.affiliation: Department of Ophthalmology, Schepens Eye Research Institute, Mass Eye and Ear Infirmary, Harvard Medical School, 20 Staniford Street, Boston, MA, 02114, USA. Joseph_Arboleda@meei.harvard.edu.
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Arboleda-Velasquez
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D020286:Pericytes / Q000166:cytology*; D010957:Plasmids / Q000235:genetics; D012171:Retinal Vessels / Q000166:cytology*; D014162:Transfection / Q000379:methods*
chemical_list: D008081:Liposomes; D049452:Green Fluorescent Proteins
keywords: Bovine; Isolation; Pericyte; Primary culture; Retina