title: Rebooting the microbiome.
abstract: Using a murine Salmonella model of colitis, we recently reported that mice receiving a community of defined gut microbiota (MET-1) lost less weight, had reduced systemic inflammation and splenic S. typhimurium infection, and decreased neutrophil infiltration in the cecum, compared to vehicle controls. In addition, animals receiving MET-1 exhibited preserved tight junction protein expression (Zonula occludens-1, claudin-1), suggesting important effects on barrier function. In this addendum, we describe additional in vitro experiments examining effects of MET-1, as well as in vivo experiments demonstrating that MET-1 is protective in a DSS model of colitis after administration of antibiotics. Placed in the context of our findings and those of others, we discuss differences in our findings between the Salmonella colitis and DSS colitis models, provide speculation as to which bacteria may be important in the protective effects of MET-1, and discuss potential implications for other GI diseases such as IBD.
authors.affiliation: a Department of Medicine , Division of Infectious Diseases/GI Diseases Research Unit, Queen's University , Kingston , ON , Canada.
authors.forename: Sean
authors.initials: S
authors.lastname: Munoz
authors.affiliation: a Department of Medicine , Division of Infectious Diseases/GI Diseases Research Unit, Queen's University , Kingston , ON , Canada.
authors.forename: Mabel
authors.initials: M
authors.lastname: Guzman-Rodriguez
authors.affiliation: b Department of Medicine , Division of Gastroenterology and Hepatology, University of Illinois at Chicago , Chicago , IL , USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.affiliation: b Department of Medicine , Division of Gastroenterology and Hepatology, University of Illinois at Chicago , Chicago , IL , USA.
authors.forename: Yong-Guo
authors.initials: YG
authors.lastname: Zhang
authors.affiliation: a Department of Medicine , Division of Infectious Diseases/GI Diseases Research Unit, Queen's University , Kingston , ON , Canada.
authors.forename: Curtis
authors.initials: C
authors.lastname: Noordhof
authors.affiliation: a Department of Medicine , Division of Infectious Diseases/GI Diseases Research Unit, Queen's University , Kingston , ON , Canada.
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: He
authors.affiliation: c Department of Molecular & Cellular Biology , University of Guelph , Guelph , ON , Canada.
authors.forename: Emma
authors.initials: E
authors.lastname: Allen-Vercoe
authors.affiliation: d Department of Pediatrics and Medicine , University of Chicago , Chicago , IL , USA.
authors.forename: Erika C
authors.initials: EC
authors.lastname: Claud
authors.affiliation: a Department of Medicine , Division of Infectious Diseases/GI Diseases Research Unit, Queen's University , Kingston , ON , Canada.
authors.forename: Elaine O
authors.initials: EO
authors.lastname: Petrof
mesh_terms: D000818:Animals; D016360:Clostridioides difficile / Q000502:physiology; D003015:Clostridium Infections / Q000382:microbiology* / Q000628:therapy; D003092:Colitis / Q000382:microbiology* / Q000628:therapy; D000069196:Gastrointestinal Microbiome; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL
keywords: DSS; epithelial barrier; microbial ecosystem therapeutic; microbiota; salmonella