title: Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae.
abstract: In this review, we provide an overview of protein synthesis in the yeast Saccharomyces cerevisiae The mechanism of protein synthesis is well conserved between yeast and other eukaryotes, and molecular genetic studies in budding yeast have provided critical insights into the fundamental process of translation as well as its regulation. The review focuses on the initiation and elongation phases of protein synthesis with descriptions of the roles of translation initiation and elongation factors that assist the ribosome in binding the messenger RNA (mRNA), selecting the start codon, and synthesizing the polypeptide. We also examine mechanisms of translational control highlighting the mRNA cap-binding proteins and the regulation of GCN4 and CPA1 mRNAs.
authors.affiliation: Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 tdever@nih.gov kinzytg@rwjms.rutgers.edu graham.pavitt@manchester.ac.uk.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Dever
authors.affiliation: Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854.
authors.forename: Terri Goss
authors.identifier: 0000-0003-1394-9226
authors.initials: TG
authors.lastname: Kinzy
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom.
authors.forename: Graham D
authors.identifier: 0000-0002-8593-2418
authors.initials: GD
authors.lastname: Pavitt
mesh_terms: D015966:Gene Expression Regulation, Fungal; D010441:Peptide Chain Elongation, Translational; D010443:Peptide Chain Termination, Translational; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: GCN4; translation elongation; translation initiation