title: Effects of thymoquinone against cisplatin-induced cardiac injury in rats.
abstract: OBJECTIVE
  T o investigate the possible protective effect of thymoquinone (TQ) in cisplatin (CP) induced myocardial injury.
  
  
  METHODS
  A total of 28 adult male Wistar-Albino rats were randomly and equally divided into four groups as follows: Group 1 (control), Group 2 (CP at 15 mg/kg dose), Group 3 (TQ 40 mg/kg/day for two days prior to CP injection and on third day, CP at 15 mg/kg dose was intraperitoneally administered and TQ treatment continued until fifth day) and Group 4 (TQ at 40mg/kg/day dose for five days).
  
  
  RESULTS
  There was a significant increment in CP group in terms of congestion, edema and pycnotic nuclei in myocardial fibers, comparing with other groups. TQ group exhibited significant increase in expression of antiapoptotic protein Bcl-2, comparing with CP group (p<0.05). In only CP administered group, expression of antiapoptotic protein Bcl-2 was lowest comparing with other groups.
  
  
  CONCLUSIONS
  Established data indicate that cisplatin is cardiotoxic and thymoquinone may be useful in treating CP-induced cardiac injury.
authors.affiliation: Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Fahri
authors.initials: F
authors.lastname: Adalı
authors.affiliation: Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Yucel
authors.initials: Y
authors.lastname: Gonul
authors.affiliation: Faculty of Medicine, Dumlupınar University, Kütahya, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kocak
authors.affiliation: Department of Embryology, Zekai Tahir Burak Women's Health Education and Research Hospital, Ankara, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Yuksel
authors.affiliation: Department of Cardiology, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Gulay
authors.initials: G
authors.lastname: Ozkececi
authors.affiliation: Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Ozdemir
authors.affiliation: Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Kamil
authors.initials: K
authors.lastname: Tunay
authors.affiliation: Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Mehmet Fatih
authors.initials: MF
authors.lastname: Bozkurt
authors.affiliation: Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Ozlem Gulec
authors.initials: OG
authors.lastname: Sen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D016227:Benzoquinones / Q000494:pharmacology* / Q000627:therapeutic use; D009202:Cardiomyopathies / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D066126:Cardiotoxicity / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D002945:Cisplatin / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000187:drug effects; D011897:Random Allocation; D017208:Rats, Wistar; D012016:Reference Values; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D016227:Benzoquinones; D019253:Proto-Oncogene Proteins c-bcl-2; C003466:thymoquinone; D002945:Cisplatin