title: Hepatitis B plasmonic biosensor for the analysis of clinical serum samples.
abstract: A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: riedel@imc.cas.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Riedel
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Surman
authors.affiliation: Biosensor Technologies, AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Simone
authors.initials: S
authors.lastname: Hageneder
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Ognen
authors.initials: O
authors.lastname: Pop-Georgievski
authors.affiliation: Molecular Diagnostics, Health and Environment Dpt., AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Christa
authors.initials: C
authors.lastname: Noehammer
authors.affiliation: Molecular Diagnostics, Health and Environment Dpt., AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Manuela
authors.initials: M
authors.lastname: Hofner
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Eduard
authors.initials: E
authors.lastname: Brynda
authors.affiliation: Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic; DWI - Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Forckenbeckstraße 50, 52074 Aachen, Germany. Electronic address: rodriguez@dwi.rwth-aachen.de.
authors.forename: Cesar
authors.initials: C
authors.lastname: Rodriguez-Emmenegger
authors.affiliation: Biosensor Technologies, AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. Electronic address: jakub.dostalek@ait.ac.at.
authors.forename: Jakub
authors.initials: J
authors.lastname: Dostálek
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D004867:Equipment Design; D006509:Hepatitis B / Q000097:blood* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D057230:Limit of Detection; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens; C045985:methacrylamide
keywords: Antifouling; Biosensors; Blood serum; Hepatitis B; Polymer brushes; Surface plasmon resonance