title: DNA barcoding of Amblyceps congeners (Siluriformes: Amblycipitidae) from Brahmaputra drainage in northeast India.
abstract: The genus Amblyceps is recognized with 18 valid species inhabiting in fast flowing rivers mostly in South and Southeast Asia. We generated 10 mtCOI sequences for four species of Amblyceps from Brahmaputra River drainage that along with conspecific database sequences were analysed in MEGA and Mr. Bayes while Automatic Barcode Gap Discover (ABGD) was performed at web interface. Lowest between species K2P ranged from 8.7% to 16.4% compared to highest within species K2P of only 2.2%. Molecular data clearly delineated studied Amblyceps congeners into four distinct species by all the three methods of analysis employed that was congruent with the morphology based identification at species level, except a minor morphological variation observed for A. mangois specimens. Our results agree with the recent resurrection of A. arunchalensis from synonym of A. mangois. We provide three new sequences for A. laticeps and identified a mislabelled sequence of A. apangi in database.
authors.affiliation: a Centre for DNA Taxonomy, Molecular Systematics Division , Zoological Survey of India , Kolkata , West Bengal , India.
authors.forename: Boni
authors.initials: B
authors.lastname: Amin Laskar
authors.affiliation: a Centre for DNA Taxonomy, Molecular Systematics Division , Zoological Survey of India , Kolkata , West Bengal , India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Kumar
authors.affiliation: b Center with Potential for Excellence in Biodiversity , Rajiv Gandhi University , Doimukh , Arunachal Pradesh , India.
authors.forename: Achom
authors.initials: A
authors.lastname: Darshan
authors.affiliation: a Centre for DNA Taxonomy, Molecular Systematics Division , Zoological Survey of India , Kolkata , West Bengal , India.
authors.forename: Shantanu
authors.initials: S
authors.lastname: Kundu
authors.affiliation: b Center with Potential for Excellence in Biodiversity , Rajiv Gandhi University , Doimukh , Arunachal Pradesh , India.|c Fishery and Aquatic Ecology Laboratory, Department of Zoology , Rajiv Gandhi University , Doimukh , Arunachal Pradesh , India.
authors.forename: Debangshu
authors.initials: D
authors.lastname: Narayan Das
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D002397:Catfishes / Q000145:classification* / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D054629:Genome, Mitochondrial; D007194:India; D010802:Phylogeny; D045483:Rivers; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: Amblyceps; Bayesian analysis; mtCOI; neighbor joining; ornamental fish