title: Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold.
abstract: Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH-MDH and G6pDH-LDH) through the control of NAD(+) substrate channeling by specifically shifting NAD(+) between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.|The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Ke
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.
authors.forename: Minghui
authors.initials: M
authors.lastname: Liu
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.
authors.forename: Shuoxing
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Qi
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.
authors.forename: Yuhe Renee
authors.initials: YR
authors.lastname: Yang
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.
authors.forename: Shaun
authors.initials: S
authors.lastname: Wootten
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA. hao.yan@asu.edu.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. cyyang@xmu.edu.cn.
authors.forename: Chaoyong James
authors.identifier: 0000-0002-2374-5342
authors.initials: CJ
authors.lastname: Yang
authors.affiliation: Center for Molecular Design and Biomimetics, Biodesign Institute, School of Molecular Sciences at, Arizona State University, Tempe, Arizona, 85287, USA. yan_liu@asu.edu.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
mesh_terms: D004247:DNA / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry*; D009243:NAD / Q000737:chemistry; D050997:Nanomedicine; D013379:Substrate Specificity; D058615:Synthetic Biology
chemical_list: D009097:Multienzyme Complexes; D009243:NAD; D004247:DNA
keywords: DNA origami; NADH; enzymes; self-assembly; substrate channeling