title: miR-133b inhibits glioma cell proliferation and invasion by targeting Sirt1.
abstract: MicroRNAs (miRs) are a class of small non-coding RNAs that function as mediators of gene expression. Dysregulations of miRs have been implicated in the development and progression of glioma. In the present study, we investigated the role of miR-133b in mediating the proliferation and invasion of glioma cells, and the potential mechanism. Real-time RT-PCR results showed that miR-133b expression was significantly decreased in glioma tissues compared with normal brain tissues. Luciferase reporter assay further identified silent information regulator 1 (Sirt1) as a novel direct target of miR-133b in glioma U87 cells. Overexpression of miR-133b suppressed Sirt1 expression and reduced the proliferation and invasion of U87 cells, which could be partly rescued by forced expression of Sirt1. In addition, the Sirt1 mRNA level was significantly higher in glioma tissues than in normal brain tissues, and was inversely correlated with miR-133b level in glioma tissues. In summary, our study sheds light on the regulatory mechanism of miR-133b in glioma growth and metastasis via direct mediation of Sirt1 expression, and suggests that Sirt1 may serve as a potential therapeutic target for glioma.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Chuntao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Zhenyan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, 410008 Hunan, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C548234:MIRN133 microRNA, human; D035683:MicroRNAs; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: glioma; invasion; microRNA-133b; proliferation; silent information regulator 1