title: Distinct characteristics of nasal polyps with and without eosinophilia.
abstract: BACKGROUND
  Eosinophilic and noneosinophilic Nasal polyps (NPs) are different subtypes of NPs and require different treatment methods.
  
  
  OBJECTIVE
  To compare the histologic characteristics, mRNA and protein expression between Nasal Polyps with and without eosinophilia.
  
  
  METHODS
  NPs tissues were obtained from eighty-six NPs patients during surgery. Eosinophilic and noneosinophilic NPs were distinguished according to immunochemical results of the specimen. The histological, mRNA and protein expression features were compared between the two groups.
  
  
  RESULTS
  In eosinophilic NPs, we observed a significantly higher GATA-3, IL-5, IL-4, IL-13 mRNA and protein expression. In noneosinophilic NPs, IL-17, IL-23 and RORc mRNA and protein expression were increased. Immunohistochemistry tests showed, more mast cells and less neutrophils in eosinophilic NPs compared with noneosinophilic NPs. Eosinophilic NPs patient presented more severe symptom scores when compared to noneosinophilic NPs.
  
  
  CONCLUSIONS
  We demonstrate for the first time that Th2 is the predominant reaction in eosinophilic NPs while Th17 is the predominant reaction in noneosinophilic NPs. Our study may provide new treatment strategy for NPs.
authors.affiliation: Guangzhou Medical College, Guangzhou Women and Children's Medical Center, Department of Otolaryngology, Guangzhou, China.
authors.forename: Changzhi
authors.initials: C
authors.lastname: Sun
authors.affiliation: China Three Gorges University, RenHe Hospital, Department of Otolaryngology, Yichang, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Guangzhou Medical College, Guangzhou Women and Children's Medical Center, Department of Otolaryngology, Guangzhou, China. Electronic address: meishano_o@163.com.
authors.forename: Renzhong
authors.initials: R
authors.lastname: Luo
mesh_terms: D000328:Adult; D002908:Chronic Disease; D016207:Cytokines / Q000276:immunology; D004802:Eosinophilia / Q000150:complications / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009298:Nasal Polyps / Q000150:complications / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012220:Rhinitis / Q000150:complications / Q000276:immunology*; D012720:Severity of Illness Index; D012852:Sinusitis / Q000150:complications / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014157:Transcription Factors
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: Células Th17; Células Th2; Nasal polyps; Pólipos nasais; Th17 cells; Th2 cells