title: Challenges in liquid chromatographic characterization of proteins.
abstract: Various liquid chromatographic techniques are considered standard analytical methods in proteins characterization. These methods provide essential information for drug approval, for biological and life sciences. On the other hand, there are some issues and challenges which have to be taken into account when analyzing these biopharmaceuticals. The aim of this review to summarize the most recent knowledge relating to the following topics: i) sample stability and complexity ii) adsorption problems: instrument inertness iii) adsorption problems: recovery from the stationary phase and iv) challenges in method development. This information is supposed to help practicing chromatographers in the emerging field of therapeutic protein chromatography.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, 1111 Budapest, Hungary. Electronic address: bobaly.balazs@gmail.com.
authors.forename: Balázs
authors.initials: B
authors.lastname: Bobály
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, 1111 Budapest, Hungary. Electronic address: sipkoeniko@gmail.com.
authors.forename: Enikő
authors.initials: E
authors.lastname: Sipkó
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, 1111 Budapest, Hungary. Electronic address: fekete@mail.bme.hu.
authors.forename: Jenő
authors.initials: J
authors.lastname: Fekete
mesh_terms: D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000627:therapeutic use; D059451:Biosimilar Pharmaceuticals / Q000737:chemistry / Q000627:therapeutic use; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D055808:Drug Discovery; D006801:Humans; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D000911:Antibodies, Monoclonal; D059451:Biosimilar Pharmaceuticals; D011506:Proteins
keywords: Adsorption; HIC; IEX; Method development; Protein chromatography; RPLC; SEC; Sample stability