title: In vivo evaluation of hybrid patches composed of PLA based copolymers and collagen/chondroitin sulfate for ligament tissue regeneration.
abstract: Biomaterials for soft tissues regeneration should exhibit sufficient mechanical strength, demonstrating a mechanical behavior similar to natural tissues and should also promote tissues ingrowth. This study was aimed at developing new hybrid patches for ligament tissue regeneration by synergistic incorporation of a knitted structure of degradable polymer fibers to provide mechanical strength and of a biomimetic matrix to help injured tissues regeneration. PLA- Pluronic®(PLA-P) and PLA-Tetronic®(PLA-T) new copolymers were shaped as knitted patches and were associated with collagen I (Coll) and collagen I/chondroitine-sulfate (Coll CS) 3-dimensional matrices. In vitro study using ligamentocytes showed the beneficial effects of CS on ligamentocytes proliferation. Hybrid patches were then subcutaneously implanted in rats for 4 and 12 weeks. Despite degradation, patches retained strength to answer the mechanical physiological needs. Tissue integration capacity was assessed with histological studies. We showed that copolymers, associated with collagen and chondroitin sulfate sponge, exhibited very good tissue integration and allowed neotissue synthesis after 12 weeks in vivo. To conclude, PLA-P/CollCS and PLA-T/CollCS hybrid patches in terms of structure and composition give good hopes for tendon and ligament regeneration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1778-1788, 2017.
authors.affiliation: Arficial Biopolymers Department, Max Mousseron Institute of Biomolecules (IBMM), UMR CNRS 5247, University of Montpellier, Faculty of Pharmacy, Montpellier, 34093, France.
authors.forename: Coline
authors.initials: C
authors.lastname: Pinese
authors.affiliation: National Institute of Applied Science, MATEIS, UMR 5510, INSA de Lyon, Université Claude Bernard Lyon 1, 69100, Villeurbanne, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Gagnieu
authors.affiliation: Arficial Biopolymers Department, Max Mousseron Institute of Biomolecules (IBMM), UMR CNRS 5247, University of Montpellier, Faculty of Pharmacy, Montpellier, 34093, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Nottelet
authors.affiliation: BIOM'UP, 69800, Saint Priest, France.
authors.forename: Capucine
authors.initials: C
authors.lastname: Rondot-Couzin
authors.affiliation: Arficial Biopolymers Department, Max Mousseron Institute of Biomolecules (IBMM), UMR CNRS 5247, University of Montpellier, Faculty of Pharmacy, Montpellier, 34093, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Hunger
authors.affiliation: Arficial Biopolymers Department, Max Mousseron Institute of Biomolecules (IBMM), UMR CNRS 5247, University of Montpellier, Faculty of Pharmacy, Montpellier, 34093, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Coudane
authors.affiliation: Arficial Biopolymers Department, Max Mousseron Institute of Biomolecules (IBMM), UMR CNRS 5247, University of Montpellier, Faculty of Pharmacy, Montpellier, 34093, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Garric
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D008023:Ligaments, Articular / Q000166:cytology / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D012038:Regeneration / Q000187:drug effects*
chemical_list: D011091:Polyesters; C033616:poly(lactide); D002809:Chondroitin Sulfates; D003094:Collagen
keywords: PLA-Pluronic; biomaterials; collagen; ligament; soft tissues reinforcement