title: Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of WaterborneEscherichia coli.
abstract: BacterialEscherichia coliisolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified asE. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % ofE. coliisolates. These results were coupled with significant molecular diversity of theE. colistrains revealed in BOXA1R-based rep-PCR technique.
authors.affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wolny-Koładka
authors.affiliation: Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059 Cracow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lenart-Boroń
keywords: Antibiotic resistance; Disk-diffusion method; ESBL; Escherichia coli; PCR; Water