title: Chemically Functionalized Silk for Human Bone Marrow-Derived Mesenchymal Stem Cells Proliferation and Differentiation.
abstract: To produce biocompatible, mechanically robust, and conductive materials for bone tissue engineering, chemical oxidation using sodium hyprochlorite (NaClO) was utilized to introduce carboxyl groups onto silk fibroin (SF). A final carboxyl content of 1.09 mM/g SF was obtained, corresponding to ∼47% of the primary hydroxymethyl groups on the silk. Interestingly, both infrared (IR) spectroscopy and circular dichroism (CD) spectra demonstrated that the resulting oxidized silk (OxSF) self-assembled into β-sheet structures under aqueous conditions and this contributed to the mechanical properties of the as-prepared silk-based scaffolds and the mineralized OxSF scaffolds (M-OxSF). The OxSF scaffolds had a compressive modulus of 211 ± 75 KPa in the hydrated state, 10 times higher than that of the SF scaffolds, and the modulus of the M-OxSF scaffolds was increased to 758 ± 189 KPa. Human bone marrow-derived mesenchymal stem cells (hMSCs) grown on the scaffolds during osteogenesis showed that the OxSF scaffolds supported the proliferation and differentiation of hMSCs in vitro.
authors.affiliation: Jiangsu Key Lab of Biomass-based Green Fuel & Chemicals, College of Chemical Engineering, Nanjing Forestry University , Nanjing 210037, China.|Department of Biomedical Engineering, 4 Colby Street, Tufts University , Medford, Massachusetts 02155, United States.
authors.forename: Ke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of Biomedical Engineering, 4 Colby Street, Tufts University , Medford, Massachusetts 02155, United States.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, 4 Colby Street, Tufts University , Medford, Massachusetts 02155, United States.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, 4 Colby Street, Tufts University , Medford, Massachusetts 02155, United States.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Biomedical Engineering, 4 Colby Street, Tufts University , Medford, Massachusetts 02155, United States.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.affiliation: Jiangsu Key Lab of Biomass-based Green Fuel & Chemicals, College of Chemical Engineering, Nanjing Forestry University , Nanjing 210037, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Fan
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005349:Fibroins; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D047011:Silk; D005349:Fibroins
keywords: carboxyl groups; mesenchymal stem cells; oxidation; serine; silk fibroin