title: Inhibition of the superantigenic activities of Staphylococcal enterotoxin A by an aptamer antagonist.
abstract: Staphylococcal enterotoxin A (SEA) is an important component of Staphylococcus aureus pathogenesis. SEA induces T lymphocytes activation and proliferation, resulting in the release of a large number of inflammatory cytokines. Blocking the toxic cascade triggered by SEA may be an effective strategy for the treatment of SEA-induced diseases. Through a systematic evolution of ligands by exponential enrichment process, we obtained an aptamer (S3) that could bind SEA with both high affinity and specificity, with a Kd value 36.93 ± 7.29 nM (n = 3). This aptamer antagonist effectively inhibited SEA-mediated human peripheral blood mononuclear cells proliferation and inflammatory cytokines (IFN-γ, TNF-α, IL-2 and IL-6) secretion. Moreover, PEGylated S3 significantly reduced mortality in murine lethal toxic shock models established by lipopolysaccharide-potentiated SEA. Therefore, this novel aptamer antagonist has the potential to become a new strategy for treating S. aureus infections and SEA-induced diseases.
authors.affiliation: Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, People's Republic of China; Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou 350025, People's Republic of China; Fujian University of Traditional Chinese Medicine, Fuzhou 350025, People's Republic of China; Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou 350025, People's Republic of China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.affiliation: Fujian University of Traditional Chinese Medicine, Fuzhou 350025, People's Republic of China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou 350025, People's Republic of China.
authors.forename: Xianhui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, People's Republic of China; Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou 350025, People's Republic of China; Fujian University of Traditional Chinese Medicine, Fuzhou 350025, People's Republic of China; Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou 350025, People's Republic of China.
authors.forename: Xiangyue
authors.initials: X
authors.lastname: Yang
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, People's Republic of China. Electronic address: cyyang@xmu.edu.cn.
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
authors.affiliation: Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, People's Republic of China; Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou 350025, People's Republic of China; Fujian University of Traditional Chinese Medicine, Fuzhou 350025, People's Republic of China; Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou 350025, People's Republic of China. Electronic address: lanxp@sina.com.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Lan
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D052158:Aptamers, Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D004768:Enterotoxins / Q000037:antagonists & inhibitors* / Q000276:immunology; D005260:Female; D006801:Humans; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000942:Antigens, Bacterial; D052158:Aptamers, Peptide; D004768:Enterotoxins; D008024:Ligands; C026066:enterotoxin A, Staphylococcal
keywords: Aptamer; SELEX; Staphylococcal enterotoxin A; Superantigen