title: Electrochemical studies of biocatalytic anode of sulfonated graphene/ferritin/glucose oxidase layer-by-layer biocomposite films for mediated electron transfer.
abstract: In this study, a bioanode was developed by using layer-by-layer (LBL) assembly of sulfonated graphene (SG)/ferritin (Frt)/glucose oxidase (GOx). The SG/Frt biocomposite was used as an electron transfer elevator and mediator, respectively. Glucose oxidase (GOx) from Aspergillus niger was applied as a glucose oxidation biocatalyst. The electrocatalytic oxidation of glucose using GOx modified electrode increases with an increase in the concentration of glucose in the range of 10-50mM. The electrochemical measurements of the electrode was carried out by using cyclic voltammetry (CV) at different scan rates (20-100mVs(-1)) in 30mM of glucose solution prepared in 0.3M potassium ferrocyanide (K4Fe(CN)6) and linear sweep voltammetry (LSV). A saturation current density of 50±2mAcm(-2) at a scan rate of 100mVs(-1) for the oxidation of 30Mm glucose is achieved.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India. Electronic address: inamuddin@rediffmail.com.
authors.lastname: Inamuddin
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Sufia Ul
authors.initials: SU
authors.lastname: Haque
authors.affiliation: Department of Chemistry, College of Science, Building #5, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mu
authors.initials: M
authors.lastname: Naushad
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D055162:Biocatalysis; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000378:metabolism; D005293:Ferritins; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D006108:Graphite; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D006108:Graphite; D005293:Ferritins; D005949:Glucose Oxidase; D005947:Glucose
keywords: Bioanode for biofuel cell applications; Ferritin; Glucose oxidase; Layer-by layer assembly; Sulfonated graphene