title: Antioxidant and anti-aging activities of polysaccharides from Calocybe indica var. APK2.
abstract: The crude polysaccharides were extracted from the fruiting bodies of Calocybe indica (CIP). The antioxidant activities of CIP were evaluated both in vitro and in vivo. Chemical characteristics of the polysaccharides were investigated. In in vitro antioxidant assay, CIP showed noticeable 2,2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl radical scavenging activities, reducing power and lipid peroxidation inhibition. Chemical analysis showed the presence of carbohydrate, protein and the FTIR spectra revealed the presence of general characteristic absorption peak of the polysaccharides. For in vivo antioxidant activity, two different doses of CIP were orally administrated over a period of 6 weeks in a d-galactose (d-gal) induced aged mice model. Significantly lowered activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), levels of glutathione (GSH) and elevated malondialdehyde (MDA) levels were observed in brain and serum of d-galactose induced rats, when compared to control rats. Administration of CIP significantly raised the activities of SOD, CAT, GPx, levels of GSH and lowered the levels of MDA in mice brain and serum in a dose-dependent manner. The results suggested that CIP had potent antioxidant activity and could minimize the occurrence of age-associated disorders associated with involvement of free radicals.
authors.affiliation: Department of Biochemistry, School of Biosciences, Periyar University, Salem 636011, Tamil Nadu, India; Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, India. Electronic address: sudhagovindan67@gmail.com.
authors.forename: Sudha
authors.initials: S
authors.lastname: Govindan
authors.affiliation: Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, India.
authors.forename: Elizabeth Elcy Rani
authors.initials: EE
authors.lastname: Johnson
authors.affiliation: Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, India.
authors.forename: Jabapramila
authors.initials: J
authors.lastname: Christopher
authors.affiliation: Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, India.
authors.forename: Jayasakthi
authors.initials: J
authors.lastname: Shanmugam
authors.affiliation: Department of Biotechnology, CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India.
authors.forename: Vinothkumar
authors.initials: V
authors.lastname: Thirumalairaj
authors.affiliation: Department of Biochemistry, Government Arts College for Women, Krishnagiri, Tamil Nadu, India.
authors.forename: Jayanthi
authors.initials: J
authors.lastname: Gopalan
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides
keywords: Anioxidants; Anti-aging; Calocybe indica; Lipid peroxidation; Polysaccharides