title: Cold-inducible RNA binding protein regulates mucin expression induced by cold temperatures in human airway epithelial cells.
abstract: Mucus overproduction is an important manifestation of chronic airway inflammatory diseases, however, the mechanisms underlying the association between cold air and mucus overproduction remain unknown. We found that the expression of the cold-inducible RNA binding protein (CIRP) was increased in patients with chronic obstructive pulmonary disease (COPD). In the present study, we tested whether CIRP was involved in inflammatory factors and mucin5AC (MUC5AC) expression after cold stimulation and investigated the potential signaling pathways involved in this process. We found that CIRP was highly expressed in the bronchi of COPD patients. The expression of CIRP, interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) were increased, and the CIRP was localized in cytoplasm after cold stimulation. MUC5AC mRNA and protein expression levels were elevated in a temperature- and time-dependent manner after cold stimulation and were associated with the phosphorylation of ERK and NF-κB, which reflected their activation. These responses were suppressed by knockdown of CIRP with a specific siRNA or the ERK and NF-κB inhibitors. These results demonstrated that CIRP was expressed in the bronchi of human COPD patients and was involved in inflammatory factors and MUC5AC expression after cold stimulation through the ERK and NF-κB pathways.
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Road, Yuzhong District, Chongqing 400010, China.
authors.forename: DanHua
authors.initials: D
authors.lastname: Ran
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Road, Yuzhong District, Chongqing 400010, China.
authors.forename: LingXiu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Road, Yuzhong District, Chongqing 400010, China.
authors.forename: WenYue
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Road, Yuzhong District, Chongqing 400010, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Affiliated Hospital of Hainan Medical University, Haikou 570102, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Han
authors.affiliation: Affiliated Hospital of Hainan Medical University, Haikou 570102, China.
authors.forename: HuaPing
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, No. 74, Linjiang Road, Yuzhong District, Chongqing 400010, China; Affiliated Hospital of Hainan Medical University, Haikou 570102, China. Electronic address: zxd999@263.net.
authors.forename: XiangDong
authors.initials: X
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D003080:Cold Temperature; D003593:Cytoplasm / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D053583:Interleukin-1beta / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009077:Mucins / Q000378:metabolism*; D010766:Phosphorylation; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012137:Respiratory System / Q000378:metabolism*; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C491390:CIRBP protein, human; C508585:IL1B protein, human; D053583:Interleukin-1beta; D009077:Mucins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D014409:Tumor Necrosis Factor-alpha; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Cold temperature; Cold-inducible RNA binding protein; Extracellular signal-regulated kinase; Interleukin-1β; Nuclear factor κB; Tumor necrosis factor α; mucin5AC