title: The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse.
abstract: The use of corn smut for the production of recombinant vaccines has been recently implemented by our group. In this study, the stability and immunogenic properties of the corn smut-based cholera vaccine, based on the cholera toxin B subunit (CTB), were determined in mouse. The immunogenic potential of distinct corn smut CTB doses ranging from 1 to 30μg were assessed, with maximum humoral responses at both the systemic (IgG) and intestinal (IgA) levels at a dose of 15μg. The humoral response last for up to 70days after the third boost. Mice were fully protected against a challenge with cholera toxin after receiving three 15μg-doses. Remarkably, the corn smut-made vaccine retained its immunogenic activity after storage at room temperature for a period of 1year and no reduction on CTB was observed following exposure at 50°C for 2h. These data support the use of the corn smut-made CTB vaccine as a highly stable and effective immunogen and justify its evaluation in target animal models, such as piglet and sheep, as well as clinical evaluations in humans.
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Monreal-Escalante
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Navarro-Tovar
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico.
authors.forename: Amalia
authors.initials: A
authors.lastname: León-Gallo
authors.affiliation: Laboratorio de Biotecnología Molecular de Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC, Camino a la Presa de San José 2055, C.P. 78216, AP 3-74 Tangamanga, San Luis Potosí, Mexico.
authors.forename: Margarita
authors.initials: M
authors.lastname: Juárez-Montiel
authors.affiliation: Laboratorio de Biotecnología Molecular de Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC, Camino a la Presa de San José 2055, C.P. 78216, AP 3-74 Tangamanga, San Luis Potosí, Mexico.
authors.forename: Alicia
authors.initials: A
authors.lastname: Becerra-Flora
authors.affiliation: Laboratorio de Biotecnología Molecular de Plantas, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica AC, Camino a la Presa de San José 2055, C.P. 78216, AP 3-74 Tangamanga, San Luis Potosí, Mexico.
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Jiménez-Bremont
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí, 78210, Mexico. Electronic address: rosales.s@fcq.uaslp.mx.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosales-Mendoza
mesh_terms: D000818:Animals; D002771:Cholera / Q000517:prevention & control; D002772:Cholera Toxin; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D005260:Female; D000071497:Immunogenicity, Vaccine; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D014588:Ustilago / Q000378:metabolism*; D064166:Vaccine Potency; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology
chemical_list: D022121:Cholera Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D002772:Cholera Toxin
keywords: Cholera; Cholera toxin; Cold chain; Low-cost vaccine; Neutralizing antibodies; Oral immunization