title: Fatty acid composition in serum correlates with that in the liver and non-alcoholic fatty liver disease activity scores in mice fed a high-fat diet.
abstract: In this study, we investigated the correlation between the serum fatty acid composition and hepatic steatosis, inflammation, hepatocellular ballooning scores, and liver fatty acids composition in mice fed a high-fat diet. Livers were collected for non-alcoholic fatty liver disease score analysis. Fatty acid compositions were analysed by gas chromatography. Correlations were determined by Pearson correlation coefficient. Exposed to a high-fat diet, mice developed fatty liver disease with varying severity without fibrosis. The serum fatty acid variation became more severe with prolonged exposure to a high-fat diet. This variation also correlated significantly with the variation in livers, with the types of fatty acids corresponding to liver steatosis, inflammation, and hepatocellular ballooning scores. Results of this study lead to the following hypothesis: the extent of serum fatty acid variation may be a preliminary biomarker of fatty liver disease caused by high-fat intake.
authors.affiliation: Faculty of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: wangxinghe1987@163.com.
authors.forename: Xing-He
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Faculty of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: ar1228@sina.com.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Li
authors.affiliation: Faculty of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: ishfaqmuhammad03@gmail.com.
authors.forename: Ishfaq
authors.initials: I
authors.lastname: Muhammad
authors.affiliation: Faculty of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: zhangxiuying@neau.edu.cn.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat; D044943:Fatty Acid Desaturases / Q000097:blood / Q000378:metabolism; D005227:Fatty Acids / Q000097:blood* / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008813:Mice, Inbred ICR; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood* / Q000378:metabolism* / Q000473:pathology; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D044943:Fatty Acid Desaturases
keywords: Fatty acids; Gas chromatography; High-fat diet; Non-alcoholic fatty liver disease (NAFLD)