title: Clinical significance and diagnostic value of serum dickkopf-1 in patients with hepatocellular carcinoma.
abstract: BACKGROUND
  Hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide. It has been widely established that the early detection of HCC enables more treatment options and translates to improved survival.
  
  
  OBJECTIVE
  To assess the diagnostic accuracy of DKK1 as a serum protein marker for HCC by examining its diagnostic sensitivity and specificity in HCC.
  
  
  METHODS
  We analyzed data for 50 patients with hepatitis C virus (HCV) related HCC as the studied group. Twenty patients with chronic hepatitis C and 20 patients with HCV-related liver cirrhosis will serve as control group. DKK1 was measured in serum by ELISA. We used receiver operating characteristics (ROC) to calculate its diagnostic accuracy.
  
  
  RESULTS
  We assessed serum DKK1 in 90 participants: 50 with HCC (studied group), 20 with chronic HCV infection, and 20 with liver cirrhosis (as control group). Serum concentration of DKK1 was significantly higher in HCC group and values did not differ significantly between the two control groups. We performed multivariate regression analysis using AFP level, number of focal lesions, focal lesion size and Portal vein thrombosis as an independent variable. ROC curves showed the optimum diagnostic cut off was 1.5 ng/mL (sensitivity 67.5%, specificity 89.3%).
  
  
  CONCLUSIONS
  Serum DKK1 could potentially be used for early diagnosis of HCC and complement measurement of AFP in the diagnosis of HCC.
authors.affiliation: a Department of Tropical Medicine , Minya University , Minya , Egypt ;
authors.forename: Yasser M
authors.initials: YM
authors.lastname: Fouad
authors.affiliation: a Department of Tropical Medicine , Minya University , Minya , Egypt ;
authors.forename: Hala I
authors.initials: HI
authors.lastname: Mohamed
authors.affiliation: a Department of Tropical Medicine , Minya University , Minya , Egypt ;
authors.forename: Enas M
authors.initials: EM
authors.lastname: Kamal
authors.affiliation: b Department of Clinical Pathology , Minya University , Minya , Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Rasek
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D004534:Egypt; D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood / Q000473:pathology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood*; D016014:Linear Models; D008103:Liver Cirrhosis / Q000097:blood / Q000473:pathology; D008113:Liver Neoplasms / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011446:Prospective Studies; D012372:ROC Curve; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; C501126:DKK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D000509:alpha-Fetoproteins
keywords: AFP; DKK 1; HCC; hepatitis C