title: Identification of CD14 transcript in blood polymorphonuclear neutrophil leukocytes and functional variation in Holsteins.
abstract: Polymorphonuclear neutrophil (PMN) leukocytes are primary phagocytic cells of the bovine mammary gland and a first line of defense against invading pathogens during bovine mastitis infection. Cluster of differentiation 14 (CD14) is mainly expressed in macrophages and neutrophils and acts as a co-receptor that binds bacterial lipopolysaccharide (LPS) and recruits PMNs to CD14-LPS complexes in mammary epithelial cells. In this study, we identified a novel splice variant in PMNs, named CD14-SV, characterized by a deleted region from c.143-579 nt compared to the CD14 reference mRNA sequence. Moreover, a single nucleotide polymorphism (c.523 A>G) in exon 2 of CD14 was identified and found to modify the secondary structure and hydrophilicity of the CD14 protein. Association analysis also showed that the milk somatic cell score, an indicator of mastitis, of cows with the GG genotype was lower than that of cows with the AA and AG genotypes. Our findings suggest that the expression of CD14 in bovine blood PMNs is regulated by alternative splicing, and that CD14-SV is a candidate functional marker that may influence mastitis-resistance in dairy cows.
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.|Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Huang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Wang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Ju
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: H S
authors.initials: HS
authors.lastname: Hao
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Zhong
authors.affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Zhu
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D018950:Lipopolysaccharide Receptors / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008414:Mastitis, Bovine / Q000097:blood / Q000235:genetics*; D009504:Neutrophils / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: D018950:Lipopolysaccharide Receptors; D020033:Protein Isoforms; D012333:RNA, Messenger