title: Recent advances in large-scale protein interactome mapping.
abstract: Protein-protein interactions (PPIs) underlie most, if not all, cellular functions. The comprehensive mapping of these complex networks of stable and transient associations thus remains a key goal, both for systems biology-based initiatives (where it can be combined with other 'omics' data to gain a better understanding of functional pathways and networks) and for focused biological studies. Despite the significant challenges of such an undertaking, major strides have been made over the past few years. They include improvements in the computation prediction of PPIs and the literature curation of low-throughput studies of specific protein complexes, but also an increase in the deposition of high-quality data from non-biased high-throughput experimental PPI mapping strategies into publicly available databases.
authors.affiliation: Department of Cellular and Molecular Medicine, Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Virja
authors.initials: V
authors.lastname: Mehta
authors.affiliation: Department of Cellular and Molecular Medicine, Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Trinkle-Mulcahy
keywords: AP-MS; APEX; BioID; Interactome; PCP; Proteomics; XL-MS; affinity purification-mass spectrometry; cross-linking MS analysis; protein correlation profiling