title: A retrospective controlled study of thiol disulfide homeostasis as a novel marker in Crimean Congo hemorrhagic fever.
abstract: OBJECTIVE
  Crimean Congo hemorrhagic fever (CCHF) is the second most common hemorrhagic fever worldwide. This study aimed to evaluate the oxidant-antioxidant balance of patients with CCHF by detecting dynamic thiol disulfide homeostasis (TDH), which is a novel oxidative stress marker, and other molecules, including paraoxonase (PON), arylesterase (ARES), ceruloplasmin (CLP), myeloperoxidase (MPO), and catalase.
  
  
  METHODS
  This retrospective, cross-sectional, controlled study, which involved patients with CCHF and healthy volunteers, measured dynamic TDH using a novel automated method developed by Erel.
  
  
  RESULTS
  We recruited 69 adult patients with CCHF (31 females, 38 males, median age 46 years). The case fatality rate was 1.49% (1/69). Increased disulfide/native thiol and disulfide/total thiol ratios, decreased total antioxidant status (TAS), and increased total oxidant status (TOS) were found in patients with CCHF. TAS, PON, and ARES values were found to be positively correlated with both native and total thiol levels, whereas TOS and CLP were negatively correlated with both, at a significant level. MPO activity was similar in both groups.
  
  
  CONCLUSIONS
  This is the first study in the literature to evaluate dynamic TDH in CCHF. TDH shifts to the oxidative side in patients with CCHF, leading to an increase in TOS.
authors.affiliation: a Infectious Diseases and Clinical Microbiology Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Zeliha Kocak
authors.initials: ZK
authors.lastname: Tufan
authors.affiliation: a Infectious Diseases and Clinical Microbiology Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Imran
authors.initials: I
authors.lastname: Hasanoglu
authors.affiliation: b Infectious Diseases and Clinical Microbiology Department , Adiyaman University, Faculty of Medicine , Adiyaman , Turkey.
authors.forename: Servet
authors.initials: S
authors.lastname: Kolgelier
authors.affiliation: c Biochemistry Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Alisik
authors.affiliation: c Biochemistry Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Ergin
authors.affiliation: a Infectious Diseases and Clinical Microbiology Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Gul Ruhsar
authors.initials: GR
authors.lastname: Yilmaz
authors.affiliation: a Infectious Diseases and Clinical Microbiology Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Mehmet A
authors.initials: MA
authors.lastname: Tasyaran
authors.affiliation: c Biochemistry Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
authors.affiliation: a Infectious Diseases and Clinical Microbiology Department , Yildirim Beyazit University, Ankara Ataturk Training & Research Hospital , Ankara , Turkey.
authors.forename: Rahmet
authors.initials: R
authors.lastname: Guner
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D003430:Cross-Sectional Studies; D004220:Disulfides / Q000378:metabolism*; D005260:Female; D006479:Hemorrhagic Fever, Crimean / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism; D012189:Retrospective Studies; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004220:Disulfides; D013438:Sulfhydryl Compounds; D002374:Catalase; D009195:Peroxidase; D002570:Ceruloplasmin; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase
keywords: Arylesterase; Catalase; Ceruloplasmin; Oxidative stress; Paraoxonase; Total oxidant status