title: Application of multiplex PCR for Rapid and sensitive detection of human papillomaviruses in cervical cancer.
abstract: OBJECTIVE
  Reffering to an increase in cervical cancer in the recent years, rapid, sensitive and economical detection of human papillomaviruses (HPVs) as causative agents of cervical cancer is important. The traditional methods for the detection of HPVs in cervical cancer, such as pap smear, suffer from limitation and PCR has a potential to overcome the limitaitons. The purpose of present research work was to identify the five important strains of HPV (16, 18, 31, 33 and 45) simultaneously by Multiplex PCR application.
  
  
  METHODS
  Study was done on 100 cervical lesions of women. DNA was extracted from specimens by a genomic DNA purification kit. A 5-plex PCR was developed for the simultaneous detection of major HPV. Five pair of new primers was designed for detection of HPV 16, 18, 31, 33 and 45 by Multiplex PCR.
  
  
  RESULTS
  Among the 100 evaluated samples, 82 were found positive to HPVs. In the meantime the highest rate of infection was for HPV 16. Also 30 of HPV positive samples had infections with two or more HPV types.
  
  
  CONCLUSIONS
  Multiplex PCR assay used in present study can provide a rapid, sensitive and economical method for detection of viral infections and is applicable to small volumes of vaginal samples.
authors.affiliation: Fateme Zandnia, Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
authors.forename: Fateme
authors.initials: F
authors.lastname: Zandnia
authors.affiliation: Abbas Doosti, Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Doosti
authors.affiliation: Abbas Mokhtari-Farsani, Young Researchers and Elite Club, Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Mokhtari-Farsani
authors.affiliation: Mohammad Taghi Kardi, Department of Biology, University of Isfahan, Isfahan, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Kardi
authors.affiliation: Abolfazl Movafagh, Department of Medical Genetics, Pediatric Neurology Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
keywords: Biomarker; Cervical cancer; Human papillomavirus; Multiplex PCR