title: Interaction of some cardiovascular drugs with bovine serum albumin at physiological conditions using glassy carbon electrode: A new approach.
abstract: In this report, for the first time, the non-modified glassy carbon electrode was used for detection of cardiovascular drug interaction with bovine serum albumin (BSA). These interactions were tested at physiological conditions (T=37°C and pH=7.4 phosphate buffer solution) in different incubation times (0-4h) by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV). The applications of DPV for quantitative investigation of some cardiovascular drug interaction with BSA (as a model of serum albumin proteins) were discussed. The herein described approach is expected to promote the exploitation of electrochemically-based methods for the study of drug-serum albumin protein interaction which is necessary in biochemical and biosensing studies. This report may open a new window to application of electrochemical sensors towards interactions of cardiovascular drugs with BSA and human serum albumin (HAS) in the near future.
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Afsharan
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran; Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran. Electronic address: Mhmmd_hasanzadeh@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hasanzadeh
authors.affiliation: Department of Nanochemistry, Nano Technology Center and Faculty of Science, Urmia University, Urmia, Iran; Department of Chemistry, Faculty of Science, Urmia University, Urmia 57154, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Shadjou
authors.affiliation: Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Jouyban
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry; D002317:Cardiovascular Agents / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
chemical_list: D002317:Cardiovascular Agents; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D002244:Carbon
keywords: Binding; Bovine serum albumin; Cardiovascular drugs; Electrochemistry; Nano-science