title: Actions of activin A, connective tissue growth factor, hepatocyte growth factor and teratocarcinoma-derived growth factor 1 on the development of the bovine preimplantation embryo.
abstract: The reproductive tract secretes bioactive molecules collectively known as embryokines that can regulate embryonic growth and development. In the present study we tested four growth factors expressed in the endometrium for their ability to modify the development of the bovine embryo to the blastocyst stage and alter the expression of genes found to be upregulated (bone morphogenetic protein 15 (BMP15) and keratin 8, type II (KRT8)) or downregulated (NADH dehydrogenase 1 (ND1) and S100 calcium binding protein A10 (S100A10)) in embryos competent to develop to term. Zygotes were treated at Day 5 with 0.01, 0.1 or 1.0nM growth factor. The highest concentration of activin A increased the percentage of putative zygotes that developed to the blastocyst stage. Connective tissue growth factor (CTGF) increased the number of cells in the inner cell mass (ICM), decreased the trophectoderm:ICM ratio and increased blastocyst expression of KRT8 and ND1. The lowest concentration of hepatocyte growth factor (HGF) reduced the percentage of putative zygotes becoming blastocysts. Teratocarcinoma-derived growth factor 1 increased total cell number at 0.01nM and expression of S100A10 at 1.0nM, but otherwise had no effects. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development.
authors.affiliation: Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, FL32611-0910, USA.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Kannampuzha-Francis
authors.affiliation: Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, FL32611-0910, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Tribulo
authors.affiliation: Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, FL32611-0910, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hansen
mesh_terms: D028341:Activins / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology*; D002417:Cattle / Q000196:embryology* / Q000502:physiology*; D055513:Connective Tissue Growth Factor / Q000494:pharmacology / Q000502:physiology*; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006133:Growth Substances / Q000494:pharmacology / Q000502:physiology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology / Q000502:physiology*; D011247:Pregnancy
chemical_list: D006133:Growth Substances; C422403:INHBA protein, Bos taurus; D028341:Activins; D055513:Connective Tissue Growth Factor; D017228:Hepatocyte Growth Factor