title: Detoxification and function of immature tomato.
abstract: α-Tomatine and chlorophyll (a and b) decreased, and β-carotene and lycopene increased with ripening of tomatoes. α-Tomatine was localised in peel of immature green tomatoes. The dose-response curve of α-tomatine determined by WST-1 (water soluble tetrazolium) assay was the same as that by LDH (lactate dehydrogenase) assay, suggesting that the cytotoxicity of α-tomatine depends on the destruction of plasma membrane. Immature green tomatoes had little cytotoxic effect after one month-incubation with 25% ethanol or 4.5% acetate at 7°C, and α-tomatine was decomposed by crude enzymes extracted from immature green tomatoes. Immature green tomatoes incubated with 4.5% acetic acid inhibited the accumulation of lipid in adipocytes. From the above facts the detoxification and the anti-obesity effect of immature green tomatoes are expected to be controlled by the removal of peel, the enzymatic decomposition or the incubation with 4.5% acetate or 25% ethanol.
authors.affiliation: Department of Materials and Life Science, Shizuoka Institute of Science and Technology, 2200 Toyosawa, Fukuroi city, Shizuoka, Japan; Microbial Technology Laboratory, 9-50-514 Kaigan-dori, Tarumi-ku, Kobe city 655-0036, Hyogo, Japan. Electronic address: ss.yamashoji@hotmail.co.jp.
authors.forename: Shiro
authors.initials: S
authors.lastname: Yamashoji
authors.affiliation: Department of Materials and Life Science, Shizuoka Institute of Science and Technology, 2200 Toyosawa, Fukuroi city, Shizuoka, Japan; Quality Management Division, Bayer Yakuhin Ltd., 121-1 Toriino, Kouga-cho, Kouga city 520-3493, Shiga, Japan. Electronic address: moi22.ryu3ya@gmail.com.
authors.forename: Eri
authors.initials: E
authors.lastname: Onoda
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000032:analysis / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D000077194:Chlorophyll A; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D051381:Rats; D014053:Tomatine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D019207:beta Carotene / Q000032:analysis / Q000378:metabolism
chemical_list: C484751:alpha-tomatine; D019207:beta Carotene; D002734:Chlorophyll; D014053:Tomatine; D002338:Carotenoids; D000077276:Lycopene; D000077194:Chlorophyll A
keywords: Adipocyte; Immature tomato detoxification; LDH assay; WST-1 assay; α-Tomatine