title: Changes in levels of oxidative stress markers and some neuronal enzyme activities in cerebrospinal fluid of multiple sclerosis patients.
abstract: The aim of the present study was to assess cerebrospinal fluid (CSF) levels of malondialdehyde (MDA), F2 isoprostanes (8-iso-PGF2α) and total antioxidant status (TAS) in relapsing-remitting (RR) and secondary progressive (SP) course of MS and neurological controls. These parameters were correlated with brain tissue damage parameters - neuron-specific enolase and 3´,5´-cAMP-phosphodiesterase (PDE) in CSF.
  
  
  
  CSF samples were obtained from MS patients divided into two groups according to the disease severity (EDSS) - RR and SP course of MS. Control group composed of neurological diagnoses without demyelination and neurodegeneration. 8-iso-PGF2α and NSE levels in the CSF samples were determined using specific immunochemistry assays. MDA levels in the CSF were measured by HPLC method after reaction with thiobarbituric acid in acidic conditions. TAS and total PDE activity of CSF was determined spectrophotometrically.
  
  
  
  There were significant differences in CSF MDA levels between MS group and controls and also between RR and SP disease course. By contrast, CSF levels of 8-iso-PGF2α in MS group and both forms of MS were comparable to control values. In addition, the results show increased CSF levels of PDE in MS group and no changes of NSE in CSF between MS and control group.
  
  
  
  These findings point to a possibility of using the parameters of different specificity to lipid peroxidation for monitoring different stages (acute/progressive) of MS. This study support the idea, that combination of CSF markers is important for monitoring overall brain tissue pathology in MS.
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Radka
authors.initials: R
authors.lastname: Bartova
authors.affiliation: 2nd Department of Neurology, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Darina
authors.initials: D
authors.lastname: Petrlenicova
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Oresanska
authors.affiliation: 2nd Department of Neurology, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Lubica
authors.initials: L
authors.lastname: Prochazkova
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Branislav
authors.initials: B
authors.lastname: Liska
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Turecky
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
authors.forename: Monika
authors.initials: M
authors.lastname: Durfinova
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000134:cerebrospinal fluid; D018450:Disease Progression; D028441:F2-Isoprostanes / Q000134:cerebrospinal fluid*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis; D020528:Multiple Sclerosis, Chronic Progressive / Q000134:cerebrospinal fluid / Q000378:metabolism*; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000134:cerebrospinal fluid / Q000378:metabolism*; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D008315:Malondialdehyde; D015105:3',5'-Cyclic-AMP Phosphodiesterases