title: Diagnosis of tuberculosis based on the detection of a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor by immuno-PCR.
abstract: Attempts were made to enhance the sensitivity of immuno-PCR assay based on the detection of cocktail of mycobacterial antigen 85B (Rv1886c), ESAT-6 (Rv3875) and cord factor (trehalose 6,6'-dimycolate) in pulmonary and extrapulmonary TB patients. Detection of Ag85B was found to be superior to the detection of cocktail in TB patients.
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India. Electronic address: pkmehta3@hotmail.com.
authors.forename: Promod K
authors.initials: PK
authors.lastname: Mehta
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
authors.forename: Netrapal
authors.initials: N
authors.lastname: Singh
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Dharra
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
authors.forename: Bhawna
authors.initials: B
authors.lastname: Dahiya
authors.affiliation: Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Statistics, MDU, Rohtak, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Sheoran
authors.affiliation: Department of TB and Respiratory Medicine, University of Health Sciences (UHS), Rohtak, India.
authors.forename: Krishna B
authors.initials: KB
authors.lastname: Gupta
authors.affiliation: Pulmonary and Critical Care Medicine, UHS, Rohtak, India.
authors.forename: Dhruva
authors.initials: D
authors.lastname: Chaudhary
authors.affiliation: Department of Medical Electronics, Ambedkar Institute of Technology, Shakarpur, Delhi 110092, India.
authors.forename: Neeru
authors.initials: N
authors.lastname: Mehta
authors.affiliation: Vallabhbhai Patel Chest Institute (VPCI), University of Delhi, Delhi 110007, India.
authors.forename: Mandira
authors.initials: M
authors.lastname: Varma-Basil
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D003311:Cord Factors / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D003311:Cord Factors; C525005:ESAT-6 antigen, Mycobacterium leprae; C071448:antigen 85B, Mycobacterium leprae
keywords: Antigen 85B; Cord factor; ESAT-6; Immuno-PCR; Tuberculosis