title: Fatty acids composition of Caenorhabditis elegans using accurate mass GCMS-QTOF.
abstract: The free living nematode Caenorhabditis elegans is a proven model organism for lipid metabolism research. Total lipids of C. elegans were extracted using chloroform and methanol in 2:1 ratio (v/v). Fatty acids composition of the extracted total lipids was converted to their corresponding fatty acids methyl esters (FAMEs) and analyzed by gas chromatography/accurate mass quadrupole time of flight mass spectrometry using both electron ionization and chemical ionization techniques. Twenty-eight fatty acids consisting of 12 to 22 carbon atoms were identified, 65% of them were unsaturated. Fatty acids containing 12 to17 carbons were mostly saturated with stearic acid (18:0) as the major constituent. Several branched-chain fatty acids were identified. Methyl-14-methylhexadecanoate (iso- 17:0) was the major identified branched fatty acid. This is the first report to detect the intact molecular parent ions of the identified fatty acids in C. elegans using chemical ionization compared to electron ionization which produced fragmentations of the FAMEs.
authors.affiliation: a Department of Environmental and Interdisciplinary Sciences , Texas Southern University , Houston , Texas , USA.
authors.forename: Parise
authors.initials: P
authors.lastname: Henry
authors.affiliation: a Department of Environmental and Interdisciplinary Sciences , Texas Southern University , Houston , Texas , USA.
authors.forename: Olufunmilayo
authors.initials: O
authors.lastname: Owopetu
authors.affiliation: a Department of Environmental and Interdisciplinary Sciences , Texas Southern University , Houston , Texas , USA.
authors.forename: Demilade
authors.initials: D
authors.lastname: Adisa
authors.affiliation: a Department of Environmental and Interdisciplinary Sciences , Texas Southern University , Houston , Texas , USA.
authors.forename: Thao
authors.initials: T
authors.lastname: Nguyen
authors.affiliation: b Department of Chemistry , Texas Southern University , Houston , Texas , USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Anthony
authors.affiliation: a Department of Environmental and Interdisciplinary Sciences , Texas Southern University , Houston , Texas , USA.
authors.forename: David
authors.initials: D
authors.lastname: Ijoni-Animadu
authors.affiliation: a Department of Environmental and Interdisciplinary Sciences , Texas Southern University , Houston , Texas , USA.
authors.forename: Sakha
authors.initials: S
authors.lastname: Jamadar
authors.affiliation: c Department of Biology , Texas Southern University , Houston , Texas , USA.
authors.forename: Fawzia
authors.initials: F
authors.lastname: Abdel-Rahman
authors.affiliation: b Department of Chemistry , Texas Southern University , Houston , Texas , USA.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Saleh
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D050356:Lipid Metabolism
chemical_list: D005227:Fatty Acids
keywords: Biomarkers; branched fatty acids; chemical ionization; electron ionization; mass spectrometry; nematodes; omega fatty acids