title: Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation.
abstract: Dendrobium officinale is one of the most well-known traditional Chinese medicines, and polysaccharide is its main active ingredient. Many studies have investigated the synthesis and accumulation mechanisms of polysaccharide, but until recently, little was known about the molecular mechanism of how polysaccharide is synthesized because no related genes have been cloned. In this study, we cloned an alkaline/neutral invertase gene from D. officinale (DoNI) by the rapid amplification of cDNA ends (RACE) method. DoNI was 2231 bp long and contained an open reading frame that predicted a 62.8-kDa polypeptide with 554-amino acid residues. An alkaline/neutral invertase conserved domain was predicted from this deduced amino acid sequence, and DoNI had a similar deduced amino acid sequence to Setaria italica and Oryza brachyantha. We also found that DoNI expression in different tissues was closely related to DoNI activity, and more importantly, polysaccharide level. Our results indicate that DoNI is associated with polysaccharide accumulation in D. officinale.
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an, China.|Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Zhejiang Agricultural and Forestry University, Lin'an, China.
authors.forename: F
authors.initials: F
authors.lastname: Gao
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an, China.|Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Zhejiang Agricultural and Forestry University, Lin'an, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Cao
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an, China.|Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Zhejiang Agricultural and Forestry University, Lin'an, China.
authors.forename: J P
authors.initials: JP
authors.lastname: Si
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an, China.|Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Zhejiang Agricultural and Forestry University, Lin'an, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an, China.|Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Zhejiang Agricultural and Forestry University, Lin'an, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Duan
mesh_terms: D017124:Conserved Sequence; D031667:Dendrobium / Q000201:enzymology / Q000235:genetics*; D017343:Genes, Plant; D016366:Open Reading Frames; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism*; D000072417:Protein Domains; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011134:Polysaccharides; D043324:beta-Fructofuranosidase