title: A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes.
abstract: We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. Sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of E-4031, whereas others did not, which may be partly due to shallower maximum diastolic potentials. We described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations, which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically.
authors.affiliation: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan. Electronic address: fernando.lopezredondo@riken.jp.
authors.forename: Fernando
authors.initials: F
authors.lastname: López-Redondo
authors.affiliation: Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan. Electronic address: junkokuro.bip@mri.tmd.ac.jp.
authors.forename: Junko
authors.initials: J
authors.lastname: Kurokawa
authors.affiliation: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan.
authors.forename: Fumimasa
authors.initials: F
authors.lastname: Nomura
authors.affiliation: Department of Frontier Bioscience, Hosei University, Koganei, Tokyo, 184-8584, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan.
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Hamada
authors.affiliation: Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Furukawa
authors.affiliation: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yasuda
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D032383:Myocytes, Cardiac / Q000502:physiology*; D018408:Patch-Clamp Techniques; D039904:Pluripotent Stem Cells / Q000166:cytology*
keywords: Cardiomyocyte; Patch-clamp; iPS cells