title: Whither Ribosome Structure and Dynamics Research? (A Perspective).
abstract: As high-resolution cryogenic electron microscopy (cryo-EM) structures of ribosomes proliferate, at resolutions that allow atomic interactions to be visualized, this article attempts to give a perspective on the way research on ribosome structure and dynamics may be headed, and particularly the new opportunities we have gained through recent advances in cryo-EM. It is pointed out that single-molecule FRET and cryo-EM form natural complements in the characterization of ribosome dynamics and transitions among equilibrating states of in vitro translational systems.
authors.affiliation: Howard Hughes Medical Institute, Columbia University, 116th and Broadway, New York, NY 10027, USA; Department of Biochemistry and Molecular Biophysics, Columbia University, 650 W. 168th Street, New York, NY 10032, USA; Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA.
authors.forename: Joachim
authors.initials: J
authors.lastname: Frank
mesh_terms: D035843:Biomedical Research / Q000639:trends*; D020285:Cryoelectron Microscopy; D031541:Fluorescence Resonance Energy Transfer; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure*
keywords: cryo-EM; smFRET; time-resolved methods; translation