title: Binding Modes of Thioflavin T on the Surface of Amyloid Fibrils Studied by NMR.
abstract: The mechanism for the interaction of thioflavin T (ThT) with amyloid fibrils at the molecular level is not known. Here, we used (1) H NMR spectroscopy to determine the binding mode of ThT on the surface of fibrils from lysozyme and insulin. Relayed rotating-frame Overhauser enhancements in ThT were observed, indicating that the orientation of ThT is orthogonal to the fibril surface. Importantly, the assembly state of ThT on both surfaces is different. On the surface of insulin fibrils, ThT is oligomeric, as indicated by rapid (1) H spin-lattice relaxation rate in the rotating frame (R1ρ ), presumably due to intermolecular dipole-dipole interactions between ThT molecules. In contrast, ThT on the surface of lysozyme fibrils is a monomer, as indicated by slower (1) H R1ρ . These results shed new light into the mechanism for the enhancement of ThT fluorescence and may lead to more efficient detectors of amyloid assemblies, which have escaped detection by ThT in monomer form.
authors.affiliation: Carlson School of Chemistry and Biochemistry, Clark University, 950 Main St, Worcester, MA, 01610, USA.
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Ivancic
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, 19716, USA.
authors.forename: Oshini
authors.initials: O
authors.lastname: Ekanayake
authors.affiliation: Carlson School of Chemistry and Biochemistry, Clark University, 950 Main St, Worcester, MA, 01610, USA. nlazo@clarku.edu.
authors.forename: Noel D
authors.initials: ND
authors.lastname: Lazo
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D052160:Benzothiazoles; D001665:Binding Sites; D005456:Fluorescent Dyes / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D013499:Surface Properties; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D013844:Thiazoles; C009462:thioflavin T
keywords: ROESY; amyloid fibrils; fibrous proteins; fluorescent probes; thioflavin T