title: Differential contribution of endothelium-derived relaxing factors to vascular reactivity in conduit and resistance arteries from normotensive and hypertensive rats.
abstract: The endothelium contributes to the maintenance of vasodilator tone by releasing nitric oxide (NO), prostacyclin (PGI2), and endothelium-derived hyperpolarizing factor (EDHF). In hypertension, endothelium-dependent relaxation is attenuated (a phenomenon referred to as endothelial dysfunction) and contributes to the increased peripheral resistance. However, which vasodilator among NO, PGI2, and EDHF is impaired in hypertension remains largely unknown. The present study was designed to study the exact contribution of NO, PGI2, and EDHF to vascular reactivity in conduit and resistance artery, under physiological and pathological conditions. The aorta and the second-order mesenteric artery from spontaneous hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were used to measure the vasorelaxation with myograph technology, in the presence or absence of different inhibitors. The results showed that the endothelium-dependent vasodilatation in the conduit artery was mediated mainly by NO, whereas the resistant artery by NO, PGI2, and EDHF together. In hypertension, both NO-mediated relaxation in the conduit artery and NO-, PGI2-, and EDHF-mediated dilation in the resistant artery were markedly impaired. Furthermore, the endothelium-dependent and the endothelium-independent vasorelaxation in conduit artery was attenuated more pronouncedly than that in the resistant artery from hypertensive rats, suggesting that the conduit artery is more vulnerable to hypertensive condition. In conclusion, vasodilators including NO, PGI2, and EDHF contribute distinctively to endothelium-dependent relaxation in conduit and resistance artery under physiological and pathological conditions.
authors.affiliation: a Experimental Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Jiaye
authors.initials: J
authors.lastname: Jiang
authors.affiliation: b Department of Cardiology, Putuo Hospital , Shanghai University of Traditional Chinese Medicine , Shanghai , China.|c Central Laboratory, Putuo Hospital , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Jian-Pu
authors.initials: JP
authors.lastname: Zheng
authors.affiliation: a Experimental Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Experimental Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Gan
authors.affiliation: a Experimental Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: a Experimental Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: a Experimental Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Li
authors.affiliation: b Department of Cardiology, Putuo Hospital , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: a Experimental Center for Teaching and Learning , Shanghai University of Traditional Chinese Medicine , Shanghai , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ke
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology; D045462:Endothelium-Dependent Relaxing Factors / Q000378:metabolism; D011464:Epoprostenol / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects; D008955:Models, Cardiovascular; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014655:Vascular Resistance / Q000502:physiology; D014664:Vasodilation / Q000502:physiology
chemical_list: D001685:Biological Factors; D045462:Endothelium-Dependent Relaxing Factors; C067410:endothelium-dependent hyperpolarization factor; D009569:Nitric Oxide; D011464:Epoprostenol
keywords: Acetylcholine; endothelium-derived hyperpolarizing factor; nitric oxide; prostacyclin; spontaneous hypertensive rats