title: Impact of 1p/19q codeletion on the diagnosis and prognosis of different grades of meningioma.
abstract: BACKGROUND
  Meningiomas are one of the most common tumours to affect the central nervous system. Genetic mutations are important in meningeal tumourigenesis, progression and prognosis. In this study, we aimed to examine the effect of 1p/19q deletion on the diagnosis and prognosis of meningioma subtypes using the fluorescence in situ hybridization (FISH) method.
  
  
  METHODS
  Twenty-four patients with meningioma were retrospectively studied. Tumour samples were obtained from 10 typical, 11 atypical and three anaplastic malignant meningiomas. The most representative tumour sections were screened for 1p/19q deletion using the FISH method.
  
  
  RESULTS
  Of the 24 patients, eight were women (33.3%) and 16 (66.7%) were men. The mean age was 56.6 years. The higher-grade meningioma was usually seen in males and had a higher rate of deletion on 1p (p = 0.001). There was a statistically significant difference between the grades and the rate of deletion on 19q (p = 0.042) and between the grades and the rates of polysomy, monosomy and amplification on 19q (p = 0.002; p = 0.001; p = 0.002, respectively). There was no statistical difference between 1p/19q codeletion and the grades of meningioma (p > 0.05). We detected higher level of Ki-67 in the condition of codeletion but did not find a statistical difference (p = 0.0553).
  
  
  CONCLUSIONS
  Deletion on 1p, as well as deletion, polysomy, monosomy and amplification on 19q, are detected more frequently in high grade meningiomas. This amplification is most likely due to the amplification of oncogenes.
authors.affiliation: a Department of Neurosurgery , Istanbul Medeniyet University, Goztepe Education and Research Hospital , Istanbul , Turkey ;
authors.forename: Recep
authors.initials: R
authors.lastname: Basaran
authors.affiliation: b Department of Histology and Embryology , Istanbul Medeniyet University School of Medicine , Istanbul , Turkey ;
authors.forename: Serap
authors.initials: S
authors.lastname: Uslu
authors.affiliation: c Department of Pathology , Istanbul Medeniyet University, Goztepe Education and Research Hospital , Istanbul , Turkey ;
authors.forename: Berrin
authors.initials: B
authors.lastname: Gucluer
authors.affiliation: d Department of Neurosurgery , Memorial Hospital , Istanbul , Turkey ;
authors.forename: Mustafa
authors.initials: M
authors.lastname: Onoz
authors.affiliation: a Department of Neurosurgery , Istanbul Medeniyet University, Goztepe Education and Research Hospital , Istanbul , Turkey ;
authors.forename: Nejat
authors.initials: N
authors.lastname: Isik
authors.affiliation: e Department of Neurosurgery , Dr. Lutfi Kirdar Kartal Education and Research Hospital , Istanbul , Turkey ;
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tiryaki
authors.affiliation: f Department of Molecular Genetics, Acibadem University School of Medicine , Istanbul , Turkey ;
authors.forename: Cengiz
authors.initials: C
authors.lastname: Yakicier
authors.affiliation: g Department of Pathology, Acibadem University School of Medicine , Istanbul , Turkey.
authors.forename: Aydin
authors.initials: A
authors.lastname: Sav
authors.affiliation: d Department of Neurosurgery , Memorial Hospital , Istanbul , Turkey ;
authors.forename: Ilhan
authors.initials: I
authors.lastname: Elmaci
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008579:Meningioma / Q000175:diagnosis* / Q000235:genetics* / Q000628:therapy; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D019394:Ki-67 Antigen
keywords: 1p/19q; Codeletion; FISH; meningioma; oncogene; prognosis