title: Phrenic motor neuron TrkB expression is necessary for acute intermittent hypoxia-induced phrenic long-term facilitation.
abstract: Phrenic long-term facilitation (pLTF) is a form of hypoxia-induced spinal respiratory motor plasticity that requires new synthesis of brain derived neurotrophic factor (BDNF) and activation of its high-affinity receptor, tropomyosin receptor kinase B (TrkB). Since the cellular location of relevant TrkB receptors is not known, we utilized intrapleural siRNA injections to selectively knock down TrkB receptor protein within phrenic motor neurons. TrkB receptors within phrenic motor neurons are necessary for BDNF-dependent acute intermittent hypoxia-induced pLTF, demonstrating that phrenic motor neurons are a critical site of respiratory motor plasticity.
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, United States.
authors.forename: Erica A
authors.initials: EA
authors.lastname: Dale
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, United States; Department of Physical Therapy and McKnight Brain Institute, University of Florida, Gainesville, FL 32610, United States.
authors.forename: Daryl P
authors.initials: DP
authors.lastname: Fields
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Devinney
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, United States; Department of Physical Therapy and McKnight Brain Institute, University of Florida, Gainesville, FL 32610, United States. Electronic address: gsmitche@phhp.ufl.edu.
authors.forename: Gordon S
authors.initials: GS
authors.lastname: Mitchell
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology; D002772:Cholera Toxin / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D000860:Hypoxia; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism*; D010791:Phrenic Nerve / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D014628:Vagotomy
chemical_list: D000276:Adjuvants, Immunologic; D034741:RNA, Small Interfering; D002245:Carbon Dioxide; D002772:Cholera Toxin; D020813:Receptor, trkB
keywords: Intermittent hypoxia; Motor neuron; Phrenic; Plasticity; RNA interference; TrkB