title: MMP3 -1171 5A/6A Promoter Genotype Influences Serum MMP3 Levels and Is Associated with Deep Venous Thrombosis.
abstract: BACKGROUND
  The aim of this study was to investigate the roles of MMP3 (matrix metalloproteinase-3) gene polymorphism and protein expression in deep venous thrombosis (DVT) among Chinese Han population.
  
  
  METHODS
  A total of 280 subjects were included in this study and categorized as case group (144 DVT patients) and control group (136 healthy individuals). Polymerase chain reaction-restriction fragment length polymorphism was used to detect MMP3 promoter -1171 5A>6A genotype and allele frequencies. MMP3 serum levels were measured by enzyme-linked immunosorbent assay. SPSS version 18.0 statistical software was used for data analysis.
  
  
  RESULTS
  There was significant difference in genotype frequencies of MMP3 gene -1171 5A>6A between the case group and the control group (all P < 0.05). Furthermore, the 6A allele on MMP3 -1171 5A>6A may be associated with increased risk of DVT (odds ratio 1.961, 95% confidence interval 1.309-2.939, P < 0.01). The MMP3 serum level in DVT patients was markedly higher than the control group (case group: 28.45 ± 10.97 vs.
  
  
  METHODS
  18.18 ± 9.03, P < 0.05). Serum MMP3 level in DVT patients carrying 5A/6A and 6A/6A genotypes was higher than the control group (P < 0.05). The bilateral calf circumference difference was significantly higher in DVT patients than the control group among all the genotypes at MMP3 gene -1171 5A>6A (all P < 0.05).
  
  
  CONCLUSIONS
  MMP3 gene -1171 5A>6A polymorphism and upregulated protein expression may be associated with DVT risk in Chinese Han population.
authors.affiliation: Department of Emergency Orthopedics, Linyi People's Hospital of Shandong Province, Linyi, Shandong, P.R. China.
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Li
authors.affiliation: Department of Spine Surgery, North Medical District, Linyi People's Hospital of Shandong Province, Linyi, Shandong, P.R. China.
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Wan
authors.affiliation: Department of Internal Medicine, Dai Gu Hospital of Mengyin County, Linyi, Shandong, P.R. China.
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Linyi People's Hospital of Shandong Province, Linyi, Shandong, P.R. China. Electronic address: lijingfang1021@163.com.
authors.forename: Jing-Fang
authors.initials: JF
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D015854:Up-Regulation; D020246:Venous Thrombosis / Q000097:blood / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000235:genetics*; D055815:Young Adult
chemical_list: C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3