title: Mouse Xenograft Model for Intraperitoneal Administration of NK Cell Immunotherapy for Ovarian Cancer.
abstract: Natural killer (NK) cells are an attractive cell population for immunotherapy. Adoptive transfer of NK cells has been tested in multiple clinical trials including acute myeloid leukemia (AML) and ovarian cancer, although limitations do exist especially for treatment of solid tumors. In order to overcome these limitations, mouse xenograft models are needed for evaluation of various NK cell populations, as well as routes of NK cell administration. Here, we describe the methods used for the establishment of an intraperitoneal (ip) ovarian cancer mouse xenograft model with ip delivery of NK cells. This model has been successfully employed with multiple ovarian cell lines and could be applied to other tumor models where the tumor's primary location is in the peritoneal cavity. It is also compatible with multiple routes of NK cell administration. Bioluminescent imaging for monitoring tumor formation and response provides for easy visualization of NK cell tumor inhibition. This xenograft model is superior to other models because the tumor is implanted into the same physiological space where ovarian cancer is found, which allows for improved mimicking of actual disease.
authors.affiliation: Department of Medicine, University of Minnesota as institution, La Jolla, CA, 92093, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Hermanson
authors.affiliation: Department of Medicine, University of Minnesota as institution, La Jolla, CA, 92093, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Bendzick
authors.affiliation: Department of Medicine, University of California- San Diego, Sanford Consortium, La Jolla, CA, 92093, USA. dskaufman@ucsd.edu.
authors.forename: Dan S
authors.initials: DS
authors.lastname: Kaufman
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007167:Immunotherapy; D007274:Injections, Intraperitoneal; D007376:Interleukin-2 / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology / Q000637:transplantation*; D008163:Luminescent Measurements; D051379:Mice; D010051:Ovarian Neoplasms / Q000276:immunology / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D007376:Interleukin-2
keywords: Bioluminescent imaging; Immunotherapy; Mouse xenograft; Natural killer cells; Ovarian cancer