title: Sequence Variations in the Non-Coding Sequence of CTX Phages in Vibrio cholerae.
abstract: This study focused on the variations in the non-coding sequences between ctxB and rstR of various CTX phages. The non-coding sequences of CTX-1 and CTX-cla are phage type-specific. The length of the non-coding region of CTX-1 and CTX-cla is 601 and 730 nucleotides, respectively. The non-coding sequence of CTX phage could be divided into three regions. There is a phage type-specific Variable region between two homologous Common regions (Common regions 1 and 2). The non-coding sequence of RS1 element is similar to CTX-1 except that Common region 1 is replaced by a short RS1-specific sequence. The non-coding sequences of CTX-2 and CTX-cla are homologous, indicating the non-coding sequence of CTX-2 is derived from CTX-cla. The non-coding region of CTX-O139 is similar to CTX-cla and CTX-2; however, it contains an extra phage type-specific sequence between Common region 2 and rstR. The variations in the non-coding sequences of CTX phages might be associated with the difference in the replication efficiency and the directionality in the integration into the V. cholerae chromosome.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Hanyang University, Ansan 15588, Republic of Korea.|Institute of Pharmacological Research, Hanyang University, Ansan 15588, Republic of Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, Hanyang University, Ansan 15588, Republic of Korea.|Institute of Pharmacological Research, Hanyang University, Ansan 15588, Republic of Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, College of Pharmacy, Hanyang University, Ansan 15588, Republic of Korea.|Institute of Pharmacological Research, Hanyang University, Ansan 15588, Republic of Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D002772:Cholera Toxin / Q000235:genetics*; D004279:DNA, Viral; D014644:Genetic Variation; D039002:Prophages / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014734:Vibrio cholerae / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D022661:RNA, Untranslated; D012097:Repressor Proteins; C107871:RstR protein, Vibrio cholerae; D014764:Viral Proteins; D002772:Cholera Toxin
keywords: CTX phage; V. cholerae; non-coding sequence