title: On the electromigration of charged fluorophore-labeled oligosaccharides in polyethylene oxide solutions.
abstract: The separation mechanism of charged fluorophore (aminopyrenetrisulfonate)-labeled maltooligosaccharides with α1-4 linkages was studied in polyethylene oxide (PEO) solutions (MW 300 000 Da) with special interest to possible analyte and/or network deformations as well as potential solute-matrix interactions. The electrophoretic mobilities of the 8-aminopyrene-1,3,6-trisulfonate-labeled maltooligosaccharides were found proportional with their MW(-2/3) . The Arrhenius function was used to determine the activation energy needed by the labeled sugars to migrate through the separation media. With increasing solute size, the activation energy (Ea ) values decreased in polymer concentrations above the entanglement threshold of the PEO, while showed apparently independent function at the entanglement threshold. The observed phenomenon was considered as a result of solute-matrix interaction, which could be alleviated by the addition of an organic modifier to the BGE.
authors.affiliation: Horváth Csaba Laboratory of Bioseparation Sciences, Regional Centre for Molecular Medicine, University of Debrecen, Debrecen, Hungary.|MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary.
authors.forename: Gabor
authors.initials: G
authors.lastname: Jarvas
authors.affiliation: Horváth Csaba Laboratory of Bioseparation Sciences, Regional Centre for Molecular Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Marta
authors.initials: M
authors.lastname: Kerekgyarto
authors.affiliation: Horváth Csaba Laboratory of Bioseparation Sciences, Regional Centre for Molecular Medicine, University of Debrecen, Debrecen, Hungary. guttman@mik.uni-pannon.hu.|MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary. guttman@mik.uni-pannon.hu.
authors.forename: András
authors.initials: A
authors.lastname: Guttman
mesh_terms: D019075:Electrophoresis, Capillary; D005456:Fluorescent Dyes / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012996:Solutions
chemical_list: D005456:Fluorescent Dyes; D009844:Oligosaccharides; D012996:Solutions; D011092:Polyethylene Glycols
keywords: Capillary electrophoresis; Polyethylene oxide; Polymer network