title: Non toxic, antibacterial, biodegradable hydrogels with pH-stimuli sensitivity: Investigation of swelling parameters.
abstract: In this work, a series of pH-sensitive hydrogels were synthesized from Sodium alginate (SA), Ethylene glycol (EG) and Acrylic acid (AA). Biodegradability of hydrogel was tested against soil burial test for 35days and in vitro phosphate buffer solution test for 10days respectively. Degradation of the sample might be due to the breakdown of ester linkage and hydrophilic pendant functionality present in hydrogel. The progression of biodegradation was examined by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Detailed swelling parameters such as swelling equilibrium Seq (%) at various pH, biological fluids (distilled water (DW), physiological saline 0.89% NaCl (PS), iso-osmotic phosphate buffer at pH 7.4 (PB)) and equilibrium water content (EWC) have also been investigated, which revealed that dynamic compassion of hydrogels. The hydrogel has shown strong antibacterial activity against Escherichia coli (gram negative) and Staphylococcus aureus (gram positive) bacteria's. Cytotoxic assays, using MTT Assay in 3T3 fibroblast Cell line was performed. At 10μg/ml, cell viability was in the range of 92-94%. However, the cell viability (%) decreases with increasing concentration of sample. The synergistic effect of biodegradable hydrogels possessing excellent swelling properties, high water content, biocompatibility and wound healing tendency using in vivo test can be made as suitable candidate for biomedical applications. In vivo wound healing studies conducted on a Wister albino rat model of incision wound performed for 9days. The results revealed that more accelerated wound healing have been observed even in shorter duration. Thus, the synthesized hydrogel with great pH-responsiveness and excellent drug delivery may have a great opening for biomedical applications.
authors.affiliation: Department of Chemistry, Periyar University, Salem 636011, Tamilnadu, India; Department of Chemistry, C. Abdul Hakeem College of Engineering and Technology, Melvisharam 632509, Tamilnadu, India. Electronic address: srsudarsan29@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Sudarsan
authors.affiliation: Department of Chemistry, C. Abdul Hakeem College of Engineering and Technology, Melvisharam 632509, Tamilnadu, India.
authors.forename: D S
authors.initials: DS
authors.lastname: Franklin
authors.affiliation: Research and Development centre, Bharathiar University, Coimbatore 641046, Tamilnadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Sakthivel
authors.affiliation: PG & Research Department of Chemistry, Muthurangam Government Arts College (Autonomous), Vellore 632002, Tamilnadu, India. Electronic address: sai_gugan@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Guhanathan
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels
keywords: Biodegradation; Cytotoxicity; Sodium alginate; Swelling studies; pH-sensitive