title: Localization and Expression of Nucleoside Transporters ENT1 and ENT2 in Polar Cells of Intestinal Epithelium.
abstract: Сасо-2 cell line is widely used in in vitro studies of the intestinal wall permeability for drugs, but transport activity of these cells has not been thoroughly studied. We analyzed localization and expression of nucleoside transporters of the ENT family transferring a number of anticancer and antiviral drugs. Immunocytochemical staining revealed apical localization of ENT1 and integral expression of ENT2 in Caco-2 cells. Based on these data and on the value of hypoxanthine uptake by these cells, we created a mathematical model allowing quantification of transporter expression on the apical and basolateral membranes of Caco-2 cells. The discrepancy between gene and protein expression of the transporter complicating prediction of patient's sensitivity to the drug upon individual therapy selection is discussed.
authors.affiliation: BioClinicum Research and Development Center, Moscow, Russia. n.seniavina@bioclinicum.com.
authors.forename: N V
authors.initials: NV
authors.lastname: Senyavina
authors.affiliation: BioClinicum Research and Development Center, Moscow, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Gerasimenko
authors.affiliation: National Medical Research Radiological Center, Moscow, Russia.
authors.forename: K A
authors.initials: KA
authors.lastname: Fomicheva
authors.affiliation: Moscow State University of Mechanical Engineering (MAMI), Moscow, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Tonevitskaya
authors.affiliation: BioClinicum Research and Development Center, Moscow, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Kaprin
mesh_terms: D000465:Algorithms; D018938:Caco-2 Cells; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D033721:Equilibrative Nucleoside Transporter 1 / Q000235:genetics / Q000378:metabolism*; D033722:Equilibrative-Nucleoside Transporter 2 / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008954:Models, Biological; D021381:Protein Transport
chemical_list: D033721:Equilibrative Nucleoside Transporter 1; D033722:Equilibrative-Nucleoside Transporter 2; C438649:SLC29A1 protein, human; C438654:SLC29A2 protein, human
keywords: Caco-2; mathematical model; nucleoside transporters; polarity