title: Local effect of bisphenol A on the estradiol synthesis of ovarian granulosa cells from PCOS.
abstract: Close relationship between polycystic ovary syndrome (PCOS) and bisphenol A (BPA) has drawn much attention in recent years, while the underlying mechanisms are poorly understood. In our study, we aim to detect BPA concentration in the follicular fluid and investigate its effect on estradiol synthesis in human granulosa cells from PCOS and non-PCOS patients. Follicular fluid and granulosa cells were collected from women who underwent controlled ovarian stimulation for in vitro fertilization or intracytoplasmic sperm injection. BPA concentration in the follicular fluid from PCOS patients (440.50 ± 63.70 pg/ml) was significantly higher than that from non-PCOS patients (338.00 ± 57.88 pg/ml). Expression of aromatase and estradiol synthesis in cultured granulosa cells was examined after treatment with BPA from 0.01 to 1 μM for 24 h. Expression of aromatase and estradiol synthesis was downregulated by BPA in a dose-dependent manner in PCOS, but no effect was observed in granulosa cells from non-PCOS patients. These findings provide evidence that increased BPA concentration in the follicular fluid of PCOS patients may play an important role in its pathogenesis by attenuating the expression of aromatase in granulosa cells.
authors.affiliation: a Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , P.R. China and.|b Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics , Shanghai , P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , P.R. China and.|b Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics , Shanghai , P.R. China.
authors.forename: Qinling
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: a Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , P.R. China and.|b Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics , Shanghai , P.R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Dang
authors.affiliation: a Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , P.R. China and.|b Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics , Shanghai , P.R. China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: He
authors.affiliation: a Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , P.R. China and.|b Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics , Shanghai , P.R. China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Li
authors.affiliation: a Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , P.R. China and.|b Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics , Shanghai , P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000328:Adult; D001559:Benzhydryl Compounds / Q000009:adverse effects* / Q000032:analysis; D004958:Estradiol / Q000096:biosynthesis*; D004968:Estrogens, Non-Steroidal / Q000009:adverse effects* / Q000032:analysis; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D006107:Granulosa Cells / Q000737:chemistry*; D006801:Humans; D010636:Phenols / Q000009:adverse effects* / Q000032:analysis; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004958:Estradiol; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Aromatase; PCOS; bisphenol A; follicular fluid; granulosa cells