title: Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models.
abstract: Invasion of melanoma cells from the primary tumor involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumor and normal tissue and between the peri-tumor area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumor dense areas, and in increased glycogen content in the peri-tumor areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumor as well as detecting differences between the tumor and the normal tissue.
authors.affiliation: Department of Materials Science & Engineering, University of Sheffield , Sheffield , UK.
authors.forename: Ceyla
authors.initials: C
authors.lastname: Yorucu
authors.affiliation: Renishaw plc , Gloucestershire , UK.
authors.forename: Katherine
authors.initials: K
authors.lastname: Lau
authors.affiliation: Department of Materials Science & Engineering, University of Sheffield , Sheffield , UK.
authors.forename: Shweta
authors.initials: S
authors.lastname: Mittar
authors.affiliation: Department of Materials Science & Engineering, University of Sheffield , Sheffield , UK.
authors.forename: Nicola H
authors.initials: NH
authors.lastname: Green
authors.affiliation: Department of Materials Science & Engineering, University of Sheffield , Sheffield , UK.
authors.forename: Ahtasham
authors.initials: A
authors.lastname: Raza
authors.affiliation: Department of Materials Science & Engineering, University of Sheffield , Sheffield , UK.
authors.forename: Ihtesham Ur
authors.initials: IU
authors.lastname: Rehman
authors.affiliation: Department of Materials Science & Engineering, University of Sheffield , Sheffield , UK.
authors.forename: Sheila
authors.initials: S
authors.lastname: MacNeil
keywords: Raman spectroscopy; melanoma; metastases; tissue engineering