title: Entrapment of laurel lipase in chitosan hydrogel beads.
abstract: Laurel seed lipase was entrapped within chitosan beads with ionotropic gelatin method using tripolyphosphate (TPP) as multivalent covalent counter ion. Immobilization yield was 78%. First, optimum immobilization conditions were determined, and morphology of chitosan beads was characterized by scanning electron microscopy. Optimum pH and temperature were evaluated as 6.0 and 40 °C, respectively. The immobilized beads saved about 55% of its activities at 60° while saved about 32% at 70 °C for 30 min. Vmax/Kmvalues were determined as 31.75 and 2.87 using olive oil as substrate for immobilized beads and free enzyme, respectively. Immobilized beads showed the activities during 30 days at +4 °C.
authors.affiliation: a Department of Chemistry, Faculty of Science , Trakya University , Edirne , Turkey.
authors.forename: Hulya
authors.initials: H
authors.lastname: Yagar
authors.affiliation: a Department of Chemistry, Faculty of Science , Trakya University , Edirne , Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Balkan
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry*; D006868:Hydrolysis; D019770:Lauraceae / Q000201:enzymology*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008863:Microspheres; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D020100:Hydrogels; D048271:Chitosan; D008049:Lipase
keywords: Chitosan; immobilization; ionotropic gelatin method; laurel; lipase; tripolyphosphate