title: Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions.
abstract: Background Recently, several studies have shown that microRNAs are present in high-density lipoprotein, and high-density lipoprotein-microRNA may be a promising disease biomarker. We investigated the stability of high-density lipoprotein-microRNAs in different storage conditions as this is an important issue for its application to the field of clinical research. Methods microRNAs were extracted from the high-density lipoprotein fraction that was purified from the serum. miR-135 a and miR-223, which are known to be present in high-density lipoprotein, were quantified by quantitative real-time PCR. The influence of preanalytical parameters on the analysis of high-density lipoprotein-miRNAs was examined by the effect of RNase, storage conditions, and freezing and thawing. Results The concentrations of microRNA in high-density lipoprotein were not altered by RNase A treatment (0-100 U/mL). No significant change in these microRNAs was observed after storing serum at room temperature or 4℃ for 0-24 h, and there was a similar result in the cryopreservation for up to two weeks. Also, high-density lipoprotein-microRNAs were stable for, at least, up to five freeze-thaw cycles. Conclusions These results demonstrated that high-density lipoprotein-microRNAs are relatively resistant to various storage conditions. This study provides new and important information on the stability of high-density lipoprotein-microRNAs.
authors.affiliation: 1 Department of Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: 2 Department of Hygiene, Fujita Health University School of Medicine, Toyoake, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Yamada
authors.affiliation: 3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Taromaru
authors.affiliation: 3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Kondo
authors.affiliation: 3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Ayuri
authors.initials: A
authors.lastname: Nagura
authors.affiliation: 3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Mirai
authors.initials: M
authors.lastname: Yamazaki
authors.affiliation: 4 Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University Hospital, Toyoake, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ando
authors.affiliation: 5 Department of Biochemistry, Fujita Health University School of Medicine, Toyoake, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Munetsuna
authors.affiliation: 6 Department of Public Health, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: 3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ohashi
authors.affiliation: 3 Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Teradaira
mesh_terms: D015925:Cryopreservation; D064368:Healthy Volunteers; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000302:isolation & purification; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000302:isolation & purification; D011485:Protein Binding; D020871:RNA Stability; D060888:Real-Time Polymerase Chain Reaction; D012034:Refrigeration; D012259:Ribonuclease, Pancreatic / Q000737:chemistry
chemical_list: D008075:Lipoproteins, HDL; D035683:MicroRNAs; D012259:Ribonuclease, Pancreatic; C544965:MIRN135 microRNA, human; C525067:MIR223, human
keywords: Circulating miRNA; high-density lipoprotein; high-density lipoprotein-miRNA; high-density lipoprotein-miRNA stability