title: An electrochemical immunosensor for quantitative detection of ficolin-3.
abstract: Diabetes mellitus (DM) is one of the most common metabolic disorders in the world, of which more than 90% is type-2 diabetes mellitus (T2DM). There is a rather urgent need for reliable, sensitive and quick detection techniques in clinical application of T2DM. Ficolin-3 is a potential biomarker of T2DM, because serum ficolin-3 levels are associated with insulin resistance and predict the incidence of T2DM. Herein, a sandwich-type electrochemical immunosensor was developed for the detection of ficolin-3 in human serum. Cyclic voltammetry and the amperometric current versus time were used to characterize the performance of the immunosensor. Under optimal conditions, the detection limitation of ficolin-3 was 100 ng ml(-1) and the linear dynamic range was between 2 and 50 μg ml(-1). The method has ideal accuracy, excellent stability and selectivity and has wide application prospects in clinical research.
authors.affiliation: Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, People's Republic of China. University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: San
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zeng
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Chenguang
authors.initials: C
authors.lastname: Wang
authors.forename: Jiarui
authors.initials: J
authors.lastname: Wu
authors.forename: Xianqiang
authors.initials: X
authors.lastname: Mi
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006046:Gold; D006801:Humans; D007118:Immunoassay; D037102:Lectins / Q000097:blood*; D057230:Limit of Detection; D000097604:Ficolins
chemical_list: D006046:Gold; D037102:Lectins; D000097604:Ficolins