title: Organelle-Targeted H2S Probes Enable Visualization of the Subcellular Distribution of H2S Donors.
abstract: Hydrogen sulfide (H2S) is an essential biological signaling molecule in diverse biological regulatory pathways. To provide new chemical tools for H2S imaging, we report here a fluorescent H2S detection platform (HSN2-BG) that is compatible with subcellular localization SNAP-tag fusion protein methodologies and use appropriate fusion protein constructs to demonstrate mitochondrial and lysosomal localization. We also demonstrate the efficacy of this detection platform to image endogenous H2S in Chinese hamster ovary (CHO) cells and use the developed constructs to report on the subcellular H2S distributions provided by common H2S donor molecules AP39, ADT-OH, GYY4137, and diallyltrisulfide (DATS). The developed constructs provide a platform poised to provide new insights into the subcellular distribution of common H2S donors and a useful tool for investigating H2S biochemistry.
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute. University of Oregon , Eugene, Oregon 97403, United States.
authors.forename: Leticia A
authors.initials: LA
authors.lastname: Montoya
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute. University of Oregon , Eugene, Oregon 97403, United States.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Pluth
mesh_terms: D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D003412:Cricetulus; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D015335:Molecular Probes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D015388:Organelles / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D006862:Hydrogen Sulfide