title: Structure characterization, chemical and enzymatic degradation, and chain conformation of an acidic polysaccharide from Lycium barbarum L.
abstract: An acidic polysaccharide, named as p-LBP, was isolated from Lycium barbarum L. by water extraction and purified by decoloration, ion exchange chromatography, dialysis and gel chromatography, successively. The primary structure analysis was determined by HPAEC-PAD, HPSEC, FT-IR, GC-MS, and NMR. The results showed p-LBP was a homogeneous heteropolysaccharide as a pectin molecule with an average molecular weight of 64kDa p-LBP was an approximately 87nm hollow sphere in 0.05mol/L sodium sulfate solution determined by HPSEC-MALLS, DLS and TEM. A discussion of degradation patterns gave the detailed structural information of p-LBP. Therefore, the results from degraded fragments elucidated that the backbone of p-LBP was formed by →4-α-GalpA-(1→, repeatedly. Partial region was connected by →4-α-GalpA-(1→ and →2-α-Rhap-(1→, alternatively. On the C-4 of partial →2-α-Rhap-(1→ residues existed branches forming by →4-β-Galp-(1→, →3-β-Galp-(1→ or →5-α-Araf-(1→, while on the C-6 of partial →3-β-Galp-(1→ residues existed secondary branches forming by terminal-α-Araf, terminal-β-Galp or →3-α-Araf-(1→.
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Yameng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Juping
authors.initials: J
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Critical Care Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: wbyao@cpu.edu.cn.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: xdgao@cpu.edu.cn.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Gao
mesh_terms: D032305:Lycium / Q000737:chemistry*; D008968:Molecular Conformation; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011134:Polysaccharides
keywords: Degradation pattern; Lycium barbarum L.; Polysaccharide; Structural characterization