title: Application of Nuclear Magnetic Resonance and Hybrid Methods to Structure Determination of Complex Systems.
abstract: The current main challenge of Structural Biology is to undertake the structure determination of increasingly complex systems in the attempt to better understand their biological function. As systems become more challenging, however, there is an increasing demand for the parallel use of more than one independent technique to allow pushing the frontiers of structure determination and, at the same time, obtaining independent structural validation. The combination of different Structural Biology methods has been named hybrid approaches. The aim of this review is to critically discuss the most recent examples and new developments that have allowed structure determination or experimentally-based modelling of various molecular complexes selecting them among those that combine the use of nuclear magnetic resonance and small angle scattering techniques. We provide a selective but focused account of some of the most exciting recent approaches and discuss their possible further developments.
authors.affiliation: School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK.
authors.forename: Filippo
authors.initials: F
authors.lastname: Prischi
authors.affiliation: Department of Clinical Neurosciences, King's College London, Denmark Hill Campus, London, UK. Annalisa.Pastore@crick.ac.uk.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pastore
mesh_terms: D000818:Animals; D001665:Binding Sites; D006801:Humans; D008958:Models, Molecular; D046912:Multiprotein Complexes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053838:Scattering, Small Angle; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D011994:Recombinant Proteins
keywords: Multidomain proteins; NMR; Protein-protein interactions; SAXS; Structural biology