title: Integration of a guided-mode resonance filter with microposts for in-cell protein detection.
abstract: We present an integrated microfluidic system consisting of a label-free biosensor of a guided-mode resonance filter (GMRF) and a microfluidic channel with a micropost filter. The GMRF was fabricated through replica molding using an ultraviolet-curable polymer and a plastic substrate. An array of microposts (a diameter and height of 26.5 and 56 μm, respectively, and a spacing between 7.5 and 9.5 μm), fabricated on a silicon substrate through photolithography, was used as the filter. A double-sided tape was used to laminate the GMRF and a microfluidic chip such that the integrated device provides two functions: filtration of the cell debris and quantification of the in-cell protein concentration. By measuring the changes in the resonant wavelength from the GMRF, the detection of β-actin in an unprocessed lysed cell sample was demonstrated; the cell debris was separated using the micropost filter to prevent false measurement.
authors.affiliation: Department of Mechanical Engineering, National Chiao Tung University, 1001 Ta Hsueh Rd., Hsinchu, Taiwan 30010. csh@nctu.edu.tw.
authors.forename: Yi-Kai
authors.initials: YK
authors.lastname: Tu
authors.affiliation: Department of Mechanical Engineering, National Chiao Tung University, 1001 Ta Hsueh Rd., Hsinchu, Taiwan 30010. csh@nctu.edu.tw.
authors.forename: Meng-Zhe
authors.initials: MZ
authors.lastname: Tsai
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, 1001 Ta Hsueh Rd., Hsinchu, Taiwan 30010.
authors.forename: I-Chin
authors.initials: IC
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, 1001 Ta Hsueh Rd., Hsinchu, Taiwan 30010.
authors.forename: Hsin-Yun
authors.initials: HY
authors.lastname: Hsu
authors.affiliation: Department of Mechanical Engineering, National Chiao Tung University, 1001 Ta Hsueh Rd., Hsinchu, Taiwan 30010. csh@nctu.edu.tw.
authors.forename: Cheng-Sheng
authors.initials: CS
authors.lastname: Huang
mesh_terms: D000199:Actins / Q000032:analysis; D015374:Biosensing Techniques; D004867:Equipment Design; D005374:Filtration; D057809:HEK293 Cells; D006801:Humans; D056656:Lab-On-A-Chip Devices; D044085:Microfluidics / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*; D012825:Silicon
chemical_list: D000199:Actins; D011506:Proteins; D012825:Silicon