title: The developmental effects of pentachlorophenol on zebrafish embryos during segmentation: A systematic view.
abstract: Pentachlorophenol (PCP) is a typical toxicant and prevailing pollutant whose toxicity has been broadly investigated. However, previous studies did not specifically investigate the underlying mechanisms of its developmental toxicity. Here, we chose zebrafish embryos as the model, exposed them to 2 different concentrations of PCP, and sequenced their entire transcriptomes at 10 and 24 hours post-fertilization (hpf). The sequencing analysis revealed that high concentrations of PCP elicited systematic responses at both time points. By combining the enrichment terms with single genes, the results were further analyzed using three categories: metabolism, transporters, and organogenesis. Hyperactive glycolysis was the most outstanding feature of the transcriptome at 10 hpf. The entire system seemed to be hypoxic, although hypoxia-inducible factor-1α (HIF1α) may have been suppressed by the upregulation of prolyl hydroxylase domain enzymes (PHDs). At 24 hpf, PCP primarily affected somitogenesis and lens formation probably resulting from the disruption of embryonic body plan at earlier stages. The proposed underlying toxicological mechanism of PCP was based on the crosstalk between each clue. Our study attempted to describe the developmental toxicity of environmental pollutants from a systematic view. Meanwhile, some features of gene expression profiling could serve as markers of human health or ecological risk.
authors.affiliation: Post-doctoral Research Station of Civil Engineering, Tongji University, Shanghai 200092, China.|Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Center of Shanghai Polytechnic University, Shanghai 201209, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Zhifa
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Pan
authors.affiliation: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Daqiang
authors.initials: D
authors.lastname: Yin
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000187:drug effects; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D004785:Environmental Pollutants / Q000633:toxicity*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D023421:Models, Animal; D010416:Pentachlorophenol / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D004785:Environmental Pollutants; D029961:Zebrafish Proteins; D010416:Pentachlorophenol