title: Quantitative Proteome Profiling of Street Rabies Virus-Infected Mouse Hippocampal Synaptosomes.
abstract: It is well established now that neuronal dysfunction rather than structural damage may be responsible for the development of rabies. In order to explore the underlying mechanisms in rabies virus (RABV) and synaptic dysfunctions, a quantitative proteome profiling was carried out on synaptosome samples from mice hippocampus. Synaptosome samples from mice hippocampus were isolated and confirmed by Western blot and transmission electron microscopy. Synaptosome protein content changes were quantitatively detected by Nano-LC-MS/MS. Protein functions were classified by the Gene Ontology (GO) and KEGG pathway. PSICQUIC was used to create a network. MCODE algorithm was applied to obtain subnetworks. Of these protein changes, 45 were upregulated and 14 were downregulated following RABV infection relative to non-infected (mock) synaptosomes. 28 proteins were unique to mock treatment and 12 were unique to RABV treatment. Proteins related to metabolism and synaptic vesicle showed the most changes in expression levels. Furthermore, protein-protein interaction (PPI) networks revealed that several key biological processes related to synaptic functions potentially were modulated by RABV, including energy metabolism, cytoskeleton organization, and synaptic transmission. These data will be useful for better understanding of neuronal dysfunction of rabies and provide the foundation for future research.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, Jilin, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Sun
authors.affiliation: Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences CAAS, Changchun, 132109, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Shi
authors.affiliation: Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education, College of Computer Science and Technology, Jilin University, Changchun, 130012, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, Jilin, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Dong
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, Jilin, China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, Jilin, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, Jilin, China. duan_ming@jlu.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Duan
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology / Q000821:virology; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D020543:Proteome / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011818:Rabies / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology / Q000821:virology*; D011820:Rabies virus / Q000502:physiology*; D013312:Stress, Physiological; D013574:Synaptosomes / Q000737:chemistry / Q000378:metabolism* / Q000821:virology
chemical_list: D020543:Proteome