title: Physical characteristics, antimicrobial and odontogenesis potentials of calcium silicate cement containing hinokitiol.
abstract: Hinokitiol is a natural material and it has antibacterial and anti-inflammatory effects. The purpose of this study was to evaluate the material characterization, cell viability, antibacterial and anti-inflammatory abilities of the hinokitiol-modified calcium silicate (CS) cement as a root end filling material. The setting times, diametral tensile strength (DTS) values and XRD patterns of CS cements with 0-10mM hinokitiol were examined. Then, the antibacterial effect and the expression levels of cyclooxygenase 2 (COX-2) and interleukin-1 (IL-1) of the hinokitiol-modified CS cements were evaluated. Furthermore, the cytocompatibility, the expression levels of the markers of odontoblastic differentiation, mineralized nodule formation and calcium deposition of human dental pulp cells (hDPCs) cultured on hinokitiol-modified CS cements were determined. The hinokitiol-modified CS cements had better antibacterial and anti-inflammatory abilities and cytocompatibility than non-modified CS cements. Otherwise, the hinokitiol-modified CS cements had suitable setting times and better odontoblastic potential of hDPCs. Previous report pointed out that the root-end filling materials may induce inflammatory cytokines reaction. In our study, hinokitiol-modified CS cements not only inhibited the expression level of inflammatory cytokines, but also had better cytocompatibility, antimicrobial properties and active ability of odontoblastic differentiation of hDPCs. Therefore, the hinokitiol-modified CS cement may be a potential root end filling material for clinic.
authors.affiliation: Institute of Oral Science, Chung Shan Medical University, Taichung City, Taiwan.
authors.forename: Ming-Hsien
authors.initials: MH
authors.lastname: Huang
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung City, Taiwan.
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Shen
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung City, Taiwan.
authors.forename: Tuan-Ti
authors.initials: TT
authors.lastname: Hsu
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Tsui-Hsien
authors.initials: TH
authors.lastname: Huang
authors.affiliation: 3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung City, Taiwan. Electronic address: eviltacasi@gmail.com.
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000032:analysis / Q000378:metabolism; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013293:Enterococcus faecalis / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D039821:Monoterpenes / Q000737:chemistry* / Q000494:pharmacology; D009805:Odontogenesis / Q000187:drug effects*; D012823:Silicate Cement / Q000737:chemistry*; D014334:Tropolone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D003738:Dental Cements; D007375:Interleukin-1; D039821:Monoterpenes; D012823:Silicate Cement; D014334:Tropolone; D051546:Cyclooxygenase 2; C009479:beta-thujaplicin
keywords: Anti-bacterial; Anti-inflammatory; Hinokitiol; Human dental pulp cell; Odontogenic