title: Characterization of the in vitro assembly of FtsZ in Arthrobacter strain A3 using light scattering.
abstract: The self-assembly of FtsZ, the bacterial homolog of tubulin, plays an essential role in cell division. Light scattering technique is applied to real-time monitor the in vitro assembly of FtsZ in Arthrobacter strain A3, a newly isolated psychrotrophic bacterium. The critical concentration needed for the assembly is estimated as 6.7μM. The polymerization of FtsZ in Arthrobacter strain A3 requires both GTP and divalent metal ions, while salt is an unfavorable condition for the assembly. The FtsZ polymerizes under a wide range of pHs, with the fastest rate around pH 6.0. The FtsZ from Arthrobacter strain A3 resembles Mycobacterium tuberculosis FtsZ in terms of the dependence on divalent metal ions and the slow polymerization rate, while it is different from M. tuberculosis FtsZ considering the sensitivity to salt and pH. The comparison of FtsZ from different organisms will greatly advance our understanding of the biological role of the key cell division protein.
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China; State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Shenglong
authors.initials: S
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Hou
authors.affiliation: School of Stomatology, Lanzhou University, Lanzhou 730000, China. Electronic address: linghuiyu2016@126.com.
authors.forename: Linghui
authors.initials: L
authors.lastname: Yu
authors.affiliation: Key Laboratory of Desert & Desertification, Cold & Arid Regions Environmental & Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China. Electronic address: lzuchen@163.com.
authors.forename: Ximing
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China. Electronic address: xiaojx@lzu.edu.cn.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Xiao
mesh_terms: D001173:Arthrobacter / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D008027:Light; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D012542:Scattering, Radiation; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria
keywords: Arthrobacter strain A3; Cell division; FtsZ; In vitro assembly; Light scattering; Polymerization