title: Identification and functional analysis of SVP ortholog in herbaceous perennial plant Gentiana triflora: Implication for its multifunctional roles.
abstract: Information concerning to regulation of vegetative phase and floral initiation in herbaceous perennial plants has been limited to a few plant species. To know and compare flowering regulation in a wider range of plant species, we identified and characterized SHORT VEGETATIVE PHASE (SVP)-like genes (GtSVP-L1 and GtSVP-L2) from herbaceous perennial gentian (Gentiana triflora). Apple latent spherical virus (ALSV)-mediated silencing of the GtSVP-L1 in G. triflora seedlings resulted in early flowering and shortened vegetative phase by about one-third period of time, without vernalization. This indicated that GtSVP-L1 acts as a negative regulator of flowering and vegetative phase. Seasonal change in the expression of GtSVP was monitored in the overwinter buds (OWBs) of G. triflora. It was found that the levels of GtSVP-L1 mRNA in OWBs increased concomitantly with induction and/or maintenance of dormancy, then decreased toward release from dormancy, while that of GtSVP-L2 mRNA remained low and unchanged. These results implied that, in herbaceous perennial plants, SVP ortholog might concern to activity-dormancy control, as well as negative regulation in flowering. Practically, these results can be applicable to non-time-consuming technologies for breeding.
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamagishi
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Kume
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020-8550, Japan; Hachimantai City Floricultural Research and Development Center, Hachimantai, Iwate 028-7592, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hikage
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Haniyeh
authors.initials: H
authors.lastname: Bidadi
authors.affiliation: Cryobiofrontier Research Center, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Wakameda
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020-8550, Japan; Cryobiofrontier Research Center, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Saitoh
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020-8550, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yoshikawa
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate 020-8550, Japan; Cryobiofrontier Research Center, Iwate University, Morioka, Iwate 020-8550, Japan. Electronic address: kentsu@iwate-u.ac.jp.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Tsutsumi
mesh_terms: D000595:Amino Acid Sequence; D035264:Flowers / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D020868:Gene Silencing; D017343:Genes, Plant / Q000502:physiology*; D030012:Gentiana / Q000235:genetics / Q000502:physiology*; D057445:Plant Dormancy / Q000235:genetics / Q000502:physiology; D012621:Seasons; D036226:Seedlings / Q000254:growth & development; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D014157:Transcription Factors
keywords: Apple latent spherical virus; Dormancy; FLOWERING LOCUS T; Floral initiation; Gentiana L.; SHORT VEGETATIVE PHASE