title: ANTIOXIDANT PROPERTIES OF METHANOLIC EXTRACTS FROM THE SHOOTS AND ROOTS OF pRi-TRANSFORMED PLANTS OF REHMANNIA GLUTINOSA LIBOSCH.
abstract: The antioxidant activity of methanolic extracts derived from shoots (HR-shoots) and roots (HR-roots) of pRi-transformed Rehmannia glutinosa plants were determined. The activity was indicated by the ability of the plant extracts to inhibit superoxide anion (O2(-·)) generation and thiobarbituric acid reactive substances (TBARS) production in resting blood platelets and platelets activated by thrombin. The strongest activity was exhibited by the HR-shoot extract (50 μg/mL). The present study also examines the antioxidant properties of the plant extracts against human plasma lipid peroxidation induced by strong biological oxidants: hydrogen peroxide (H2O2) and H2O2/Fe. The study shows that extracts from transformed R. glutinosa plants may be a promising source of natural antioxidants, which would be valuable in various cardiovascular diseases. The extracts may also protect lipids against oxidative modifications.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Piatczak
authors.forename: Marta
authors.initials: M
authors.lastname: Dfbska
authors.forename: Bogdan
authors.initials: B
authors.lastname: Kontek
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
authors.forename: Halina
authors.initials: H
authors.lastname: Wysokinskai
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018506:Gene Expression Regulation, Plant; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D000432:Methanol / Q000737:chemistry*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D018520:Plant Shoots; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics*; D010946:Plants, Medicinal; D032267:Rehmannia / Q000737:chemistry / Q000235:genetics*; D012997:Solvents / Q000737:chemistry*; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016877:Oxidants; D010936:Plant Extracts; D012997:Solvents; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D006861:Hydrogen Peroxide; D013917:Thrombin; D000432:Methanol