title: Bioaccumulation of PCB-153 and effects on molecular biomarkers acetylcholinesterase, glutathione-S-transferase and glutathione peroxidase in Mytilus galloprovincialis mussels.
abstract: In this study, PCB-153 bioaccumulation kinetics and concentration-response experiments were performed employing wild Mytilus galloprovincialis mussels. In addition, the activity of three enzymatic biomarkers: glutathione S-transferase (GST), glutathione peroxidase (GPx) and acetylcholinesterase (AChE), were measured in the mussel gills. The experimental data fitted well to an asymptotic accumulation model with a high bioconcentration factor (BCF) of 9324 L kg(-1) and a very limited depuration capacity, described by a low excretion rate coefficient (Kd = 0.083 d(-1)). This study reports by first time in mussels significant inhibition of GST activity and significant induction of GPx activity as a result of exposure to dissolved PCB-153. In contrast, AChE activity was unaffected at all concentrations and exposure times tested. The effects on both enzymes are time-dependent, which stresses the difficulties inherent to the use of these biomarkers in chemical pollution monitoring programs.
authors.affiliation: Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, Estrada Colexio Universitario s/n, 36310, Vigo, Galicia, Spain. Electronic address: leticia1582@uvigo.es.
authors.forename: Leticia
authors.initials: L
authors.lastname: Vidal-Liñán
authors.affiliation: Instituto Español de Oceanografía, Centro Oceanográfico de Vigo., Apdo.1552, 36280, Vigo, Galicia, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Bellas
authors.affiliation: Instituto Español de Oceanografía, Centro Oceanográfico de Vigo., Apdo.1552, 36280, Vigo, Galicia, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Soriano
authors.affiliation: Grupo Química Analítica Aplicada (QANAP), Instituto Universitario de Medio Ambiente (IUMA), Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Química Analítica, Facultade de Ciencias, Universidade da Coruña, Campus de A Coruña, 15071 A Coruña, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Concha-Graña
authors.affiliation: Grupo Química Analítica Aplicada (QANAP), Instituto Universitario de Medio Ambiente (IUMA), Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Química Analítica, Facultade de Ciencias, Universidade da Coruña, Campus de A Coruña, 15071 A Coruña, Spain.
authors.forename: Soledad
authors.initials: S
authors.lastname: Muniategui
authors.affiliation: ECIMAT, Universidade de Vigo, Illa de Toralla s.n., 36331, Vigo, Galicia, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Beiras
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D049878:Mytilus / Q000201:enzymology / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000493:pharmacokinetics*; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; C014024:2,4,5,2',4',5'-hexachlorobiphenyl
keywords: Acetylcholinesterase; Antioxidant enzymes; Bioaccumulation; Biomarkers; Mytilus galloprovincialis; PCB-153