title: Preparation of Viscumin-Ferromagnetic Particles Conjugate and Study of Its Internalization by Human Glioblastoma A172 Cells.
abstract: Magnetite particles modified by polyethylene glycol with a molecular weight of 3 kDa and hydrodynamic diameter of ~60 nm were used. Plant lectin viscumin covalently immobilized on these nanoparticles retained its binding activity. Immunochemical characteristics of conjugated viscumin were evaluated using monoclonal antibodies. The resultant conjugate with a hydrodynamic diameter of 70 nm was used for studies of binding and internalization by target cells. Binding of viscumin and its conjugate was determined by receptors containing terminal galactose, while intracellular distribution varied. The model system presented in this study can be used for creation of drugs for target therapy.
authors.affiliation: BioClinicum Research and Technology Center, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Khutornenko
authors.affiliation: BioClinicum Research and Technology Center, Moscow, Russia.
authors.forename: V M
authors.initials: VM
authors.lastname: Gerasimov
authors.affiliation: BioClinicum Research and Technology Center, Moscow, Russia. d.sakharov@bioclinicum.com.
authors.forename: D A
authors.initials: DA
authors.lastname: Sakharov
mesh_terms: D045744:Cell Line, Tumor; D005909:Glioblastoma; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D058726:Nanoconjugates / Q000737:chemistry*; D010316:Particle Size; D054790:Ribosome Inactivating Proteins, Type 2 / Q000737:chemistry* / Q000378:metabolism; D014118:Toxins, Biological / Q000737:chemistry* / Q000378:metabolism
chemical_list: D058185:Magnetite Nanoparticles; D058726:Nanoconjugates; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488862:mistletoe lectin I
keywords: Internalization; intracellular transport; magnetite nanoparticles; target delivery; viscumin