title: ARF1 activation dissociates ADRP from lipid droplets to promote HCV assembly.
abstract: Lipid droplets are the place for HCV assembly and ADRP is an abundant lipid droplets-associated protein. However, little is known about the mechanisms how ADRP is involved in HCV life cycle. Here we demonstrate that activation of ARF1 dissociates ADRP from lipid droplets. A constitute active form of ARF1 (ARF1Q71I) promotes HCV assembly. We found that ADRP plays a positive role in HCV replication and a negative role in HCV assembly. Overexpression of ADRP increases the size of lipid droplets, while silencing ADRP reduces the size of lipid droplets. These findings provide new insight into the role of lipid droplets proteins in life cycle of HCV.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100176, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100176, China.
authors.forename: Peiqi
authors.initials: P
authors.lastname: Yin
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100176, China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100176, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100176, China. Electronic address: armzhang@hotmail.com.
authors.forename: Leiliang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism*; D002460:Cell Line; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000378:metabolism* / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D066292:Lipid Droplets / Q000378:metabolism / Q000821:virology*; D000070780:Perilipin-2 / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D000070780:Perilipin-2; D020823:ADP-Ribosylation Factor 1
keywords: ADRP; ARF1; HCV; LDs