title: Clinical and Immunoinflammatory Evaluation of One-Stage Full-Mouth Ultrasonic Debridement as a Therapeutic Approach for Smokers With Generalized Aggressive Periodontitis: A Short-Term Follow-Up Study.
abstract: This study aims to evaluate the effect of one-stage full-mouth ultrasonic debridement (OSFMUD) on clinical and immunoinflammatory parameters in smokers with generalized aggressive periodontitis (GAgP).
  
  
  
  Fourteen smoking and 14 non-smoking patients with GAgP were selected. After initial supragingival therapy, patients were treated by OSFMUD. Full-mouth parameters evaluated were: 1) plaque index (PI); 2) bleeding scores (BS); 3) probing depth (PD); and 4) clinical attachment level (CAL). Clinical evaluation was performed, and gingival crevicular fluid (GCF) was collected for selected sites (ss) at baseline and 1, 3, and 6 months. GCF was analyzed via enzyme-linked immunosorbent assay for: 1) receptor activator of nuclear factor-κ B ligand (RANKL); 2) osteoprotegerin (OPG); 3) interleukin (IL)-6; and 4) tumor necrosis factor (TNF)-α, whereas secreted osteoclastogenic factor of activated T-cells (SOFAT) was evaluated by Western blotting.
  
  
  
  Significant reduction (P <0.05) was observed between baseline and 6 months for: 1) PI; 2) BS; and 3) PD, with no difference between smoking and non-smoking patients (P >0.05). Regarding CAL, only non-smoking patients showed a significant decrease (P <0.05). Significant reduction (P <0.05) was observed in both groups for: 1) PIss; 2) PDss; 3) bleeding on probing; and 4) relative CAL. Smoking and non-smoking patients presented significantly decreased levels of IL-6 and TNF-α over time (P <0.05); however, no difference was observed between groups (P >0.05). RANKL was significantly different (P <0.05) only for non-smokers at 6 months, whereas OPG was not significant (P >0.05). SOFAT expression was significantly lower (P <0.05) after OSFMUD for non-smokers only.
  
  
  
  Considering the clinical and immunoinflammatory parameters evaluated in this short-term follow-up study, it can be concluded that OSFMUD can be used as an alternative treatment for smokers with GAgP.
authors.affiliation: Department of Molecular Biology, São Leopoldo Mandic Dental Institute and Research Center, Campinas, São Paulo, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: De Genaro Modanese
authors.affiliation: Department of Molecular Biology, São Leopoldo Mandic Dental Institute and Research Center, Campinas, São Paulo, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Tiosso-Tamburi
authors.affiliation: Department of Molecular Biology, São Leopoldo Mandic Dental Institute and Research Center, Campinas, São Paulo, Brazil.
authors.forename: Vivian Fernandes
authors.initials: VF
authors.lastname: Furletti de Goes
authors.affiliation: Department of Pharmaceutical Sciences, University of Sorocaba, Sorocaba, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: de Cássia Bergamaschi
authors.affiliation: Department of Molecular Biology, São Leopoldo Mandic Dental Institute and Research Center, Campinas, São Paulo, Brazil.
authors.forename: Elizabeth Ferreira
authors.initials: EF
authors.lastname: Martinez
authors.affiliation: Department of Molecular Biology, São Leopoldo Mandic Dental Institute and Research Center, Campinas, São Paulo, Brazil.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
authors.affiliation: Department of Molecular Biology, São Leopoldo Mandic Dental Institute and Research Center, Campinas, São Paulo, Brazil.
authors.forename: Daiane Cristina
authors.initials: DC
authors.lastname: Peruzzo
mesh_terms: D010520:Aggressive Periodontitis / Q000628:therapy*; D003646:Debridement; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid; D006801:Humans; D010512:Periodontal Index; D000074285:Smokers; D014465:Ultrasonics
keywords: Aggressive periodontitis; periodontal debridement; periodontitis; smoking