title: Characterization of bacterial communities associated with Brassica napus L. growing on a Zn-contaminated soil and their effects on root growth.
abstract: The interaction between plant growth-promoting bacteria (PGPB) and plants can enhance biomass production and metal tolerance of the host plants. This work aimed at isolating and characterizing the cultivable bacterial community associated with Brassica napus growing on a Zn-contaminated site, for selecting cultivable PGPB that might enhance biomass production and metal tolerance of energy crops. The effects of some of these bacterial strains on root growth of B. napus exposed to increasing Zn and Cd concentrations were assessed. A total of 426 morphologically different bacterial strains were isolated from the soil, the rhizosphere, and the roots and stems of B. napus. The diversity of the isolated bacterial populations was similar in rhizosphere and roots, but lower in soil and stem compartments. Burkoholderia, Alcaligenes, Agrococcus, Polaromonas, Stenotrophomonas, Serratia, Microbacterium, and Caulobacter were found as root endophytes exclusively. The inoculation of seeds with Pseudomonas sp. strains 228 and 256, and Serratia sp. strain 246 facilitated the root development of B. napus at 1,000 µM Zn. Arthrobacter sp. strain 222, Serratia sp. strain 246, and Pseudomonas sp. 228 and 262 increased the root length at 300 µM Cd.
authors.affiliation: a Departamento de Investigación Agroambiental , Alcalá de Henares , Madrid , Spain.|b Environmental Biology, Centre for Environmental Sciences, Hasselt University , Diepenbeek , Belgiu.
authors.forename: Blanca
authors.initials: B
authors.lastname: Montalbán
authors.affiliation: b Environmental Biology, Centre for Environmental Sciences, Hasselt University , Diepenbeek , Belgiu.
authors.forename: Sarah
authors.initials: S
authors.lastname: Croes
authors.affiliation: b Environmental Biology, Centre for Environmental Sciences, Hasselt University , Diepenbeek , Belgiu.
authors.forename: Nele
authors.initials: N
authors.lastname: Weyens
authors.affiliation: a Departamento de Investigación Agroambiental , Alcalá de Henares , Madrid , Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Lobo
authors.affiliation: a Departamento de Investigación Agroambiental , Alcalá de Henares , Madrid , Spain.
authors.forename: Araceli
authors.initials: A
authors.lastname: Pérez-Sanz
authors.affiliation: b Environmental Biology, Centre for Environmental Sciences, Hasselt University , Diepenbeek , Belgiu.
authors.forename: Jaco
authors.initials: J
authors.lastname: Vangronsveld
mesh_terms: D001530:Belgium; D018533:Biomass; D029688:Brassica napus / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D002104:Cadmium / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D064307:Microbiota / Q000187:drug effects / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity; D015032:Zinc / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D002104:Cadmium; D015032:Zinc
keywords: Endophytes; inoculation; phytoremediation; plant growth-promoting bacteria; plant-associated bacteria