title: Biophysical studies on the interaction of platinum(II) complex containing antiviral drug ribavirin with human serum albumin.
abstract: This study describes HSA binding properties of a platinum(II) complex with an antiviral drug ligand; ribavirin. Spectroscopic analysis of the emission quenching at different temperatures and UV-vis spectra revealed that the quenching mechanism of HSA by Pt(II) complex is static quenching mechanism. The binding constants and the number of binding sites were determined by fluorescence quenching method. Pt(II) complex binding is characterized by one high affinity binding site. Through the site marker competitive experiment, site I was assigned to possess high affinity binding site for Pt(II) complex. The calculated thermodynamic parameters (ΔG, ΔH and ΔS) confirmed that the binding reaction is spontaneous, and hydrophobic forces played a major role in the reaction. Fluorescence quenching studies showed that the binding affinity of Pt(II) complex with HSA in the buffer solution at different pH values is: Kb (pH3.0)>Kb (pH9.0)>Kb (pH7.4). The CD spectrum shows the binding of Pt(II) complex leads to a change in the α-helical structure of HSA. CD spectroscopy studies further indicated the influence of pH on the complexation process and the alteration in the protein conformation upon binding.
authors.affiliation: Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran; Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address: NahidShahabadi@yahoo.com.
authors.forename: Nahid
authors.initials: N
authors.lastname: Shahabadi
authors.affiliation: Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran; Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Saba
authors.initials: S
authors.lastname: Hadidi
authors.affiliation: Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Zeinab Mirzaei
authors.initials: ZM
authors.lastname: Kalar
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D010984:Platinum / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012254:Ribavirin / Q000737:chemistry* / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D056831:Coordination Complexes; D012709:Serum Albumin; D012254:Ribavirin; D010984:Platinum
keywords: Different pH conditions; Human serum albumin; Multi-spectroscopic methods; Pt(II) complex; Ribavirin