title: Neuroleptic drugs in the treatment of tuberculosis: Minimal inhibitory concentrations of different phenothiazines against Mycobacterium tuberculosis.
abstract: Due to an increase of drug resistant TB, alternative drugs that are not currently listed in the WHO guidelines on MDR TB treatment are currently being evaluated. Our group tested 100 susceptible, 20 MDR and 2 XDR Mtb strains against the phenothiazine derivatives thioridazine, trifluoperazine and triflupromazine. MIC testing was performed on Middlebrook 7H10 agar and was defined as the lowest drug concentration that inhibits ≥99% of the bacterial population. We confirm very good in vitro activity of phenothiazines against Mycobacterium tuberculosis. In >77% of all strains MICs of ≤10 μg/ml were found.
authors.affiliation: Department of Pneumology, Lungenklinik Heckeshorn, HELIOS Klinikum Emil von Behring, Walterhöfer Str 11, 14165 Berlin, Germany. Electronic address: silvan.vesenbeckh@helios-kliniken.de.
authors.forename: Silvan
authors.initials: S
authors.lastname: Vesenbeckh
authors.affiliation: Department of Pneumology, Lungenklinik Heckeshorn, HELIOS Klinikum Emil von Behring, Walterhöfer Str 11, 14165 Berlin, Germany. Electronic address: david.krieger@helios-kliniken.de.
authors.forename: David
authors.initials: D
authors.lastname: Krieger
authors.affiliation: Institute of Microbiology, HELIOS Klinikum Emil von Behring, Walterhöfer Str 11, 14165 Berlin, Germany. Electronic address: gudrun.bettermann@berlin.de.
authors.forename: Gudrun
authors.initials: G
authors.lastname: Bettermann
authors.affiliation: Department of Pneumology, Lungenklinik Heckeshorn, HELIOS Klinikum Emil von Behring, Walterhöfer Str 11, 14165 Berlin, Germany. Electronic address: nicolas.schoenfeld@helios-kliniken.de.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Schönfeld
authors.affiliation: Department of Pneumology, Lungenklinik Heckeshorn, HELIOS Klinikum Emil von Behring, Walterhöfer Str 11, 14165 Berlin, Germany; Deutsches Zentralkomitee zur Bekämpfung von Tuberkulose (DZK), Building Q, Walterhöfer Str 11, 14165 Berlin, Germany. Electronic address: torsten.bauer@helios-kliniken.de.
authors.forename: Torsten Thomas
authors.initials: TT
authors.lastname: Bauer
authors.affiliation: Institute of Microbiology, HELIOS Klinikum Emil von Behring, Walterhöfer Str 11, 14165 Berlin, Germany. Electronic address: holger.ruessmann@helios-kliniken.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Rüssmann
authors.affiliation: Institute of Microbiology, HELIOS Klinikum Emil von Behring, Walterhöfer Str 11, 14165 Berlin, Germany. Electronic address: herald.mauch@helios-kliniken.de.
authors.forename: Harald
authors.initials: H
authors.lastname: Mauch
mesh_terms: D014150:Antipsychotic Agents / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000494:pharmacology*; D058492:Drug Repositioning; D024901:Drug Resistance, Multiple, Bacterial; D054908:Extensively Drug-Resistant Tuberculosis / Q000188:drug therapy / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D010640:Phenothiazines / Q000494:pharmacology*; D010641:Phenotype; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D014150:Antipsychotic Agents; D000995:Antitubercular Agents; D010640:Phenothiazines
keywords: Antibiotic resistance; Drug development; Efflux pump; Guideline; Public health