title: Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients.
abstract: To examine evidence of positive antibodies against immunogenic proteins of Anaplasma phagocytophilum in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific Anaplasma antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (n = 47) and from blood donors (n = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (2·0%) showed positive IgM reactions and seven samples (2·4%) were IgG positive for A. phagocytophilum by immunoblot. Borderline reactivity was found in 30 samples (10·14%) for IgM and 36 samples (12·2%) for IgG. The difference between patients and blood donors was statistically significant for IgM (P = 0·006) and for IgG (P = 0·0007) antibodies. A statistically significant result was obtained for IgG (P = 0·02) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to A. phagocytophilum in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.
authors.affiliation: Department of Children's Infectious Diseases,Faculty of Medicine and University Hospital,Masaryk University,Brno,Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Krbková
authors.affiliation: Department of Children's Infectious Diseases,Faculty of Medicine and University Hospital,Masaryk University,Brno,Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Homola
authors.affiliation: Department of Children's Infectious Diseases,Faculty of Medicine and University Hospital,Masaryk University,Brno,Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Hlaváčová
authors.affiliation: Department of Children's Infectious Diseases,Faculty of Medicine and University Hospital,Masaryk University,Brno,Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Mikolášek
authors.affiliation: Department of Clinical Microbiology,University Hospital,Brno,Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Bednářová
authors.affiliation: Department of Clinical Biochemistry,University Hospital,Brno,Department of Laboratory Methods,Faculty of Medicine,Masaryk University,Brno,Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Čermáková
mesh_terms: D000293:Adolescent; D041081:Anaplasma phagocytophilum / Q000276:immunology*; D000712:Anaplasmosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D060085:Coinfection / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D018153:Czech Republic; D004675:Encephalitis, Tick-Borne / Q000276:immunology* / Q000382:microbiology; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000276:immunology / Q000382:microbiology*; D008297:Male; D008565:Membrane Proteins / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins
keywords: Anaplasma phagocytophilum; Lyme borreliosis; human granulocytic anaplasmosis; major surface proteins; outer membrane protein A; tick-borne encephalitis