title: The Effect of G2 Adjuvant on Gene Expression and Delivery of NKG2D Receptor on NK Cells in Peripheral Blood.
abstract: BACKGROUND
  Natural killer (NK) cells are a subset of lymphocytes in humans that release cytokines such as tumor necrosis factor alpha and interferon gamma-γ during infection. NKG2D is one of the most important stimulating NK receptors binding MIC-A, MIC-B, and ULBPs, which leads to activation of NK cells against tumor cells. In this study, the authors evaluated the effect of G2 adjuvant on gene expression and delivery of NKG2D receptor on NK cells in peripheral blood.
  
  
  METHODS
  Peripheral blood mononuclear cells were isolated from venous blood obtained from healthy volunteers after adding G2 adjuvant within 12, 24, and 48 hours of incubation. Then, total RNA was extracted from the cells, cDNA synthesis was performed, and gene expression was evaluated by real-time PCR. In addition, NK cells were stained with the appropriate monoclonal antibodies, and the receptors expressed on cell surface were quantified.
  
  
  RESULTS
  G2 adjuvant leads to upregulation of gene expression and increases the expression of NKG2D receptor on the surface of NK cells after incubation.
  
  
  CONCLUSIONS
  The findings of this study demonstrated that G2 adjuvant can increase NK cell cytotoxicity. It may play an important role in killing tumor cells, preventing tumor growth and metastasis.
authors.affiliation: 1 Cancer, Petroleum and Environmental Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences , Ahvaz, Iran .|2 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran .
authors.forename: Ali
authors.initials: A
authors.lastname: Khodadadi
authors.affiliation: 1 Cancer, Petroleum and Environmental Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences , Ahvaz, Iran .|2 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran .
authors.forename: Zahra
authors.initials: Z
authors.lastname: Abdoli
authors.affiliation: 2 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran .
authors.forename: Mehri Ghafourian
authors.initials: MG
authors.lastname: Boroujerdnia
authors.affiliation: 3 Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran .
authors.forename: Mohammad-Ali
authors.initials: MA
authors.lastname: Assarehzadegan
authors.affiliation: 1 Cancer, Petroleum and Environmental Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences , Ahvaz, Iran .|2 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran .
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ghasemi
authors.affiliation: 4 School of Public Health, Tehran University of Medical Sciences, Tehran, Iran .|5 Dr. Mohaghegh's Foundation Research on Industrial Biotechnology, Tehran, Iran .
authors.forename: Saleh Mohaghegh
authors.initials: SM
authors.lastname: Hazrati
authors.affiliation: 1 Cancer, Petroleum and Environmental Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences , Ahvaz, Iran .|2 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran .
authors.forename: Nader Dashti
authors.initials: ND
authors.lastname: Gerdabi
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000276:Adjuvants, Immunologic; C527085:KLRK1 protein, human; D055655:NK Cell Lectin-Like Receptor Subfamily K
keywords: NKG2D; cancer; flow cytometry; natural killer cells; qRT-PCR