title: Association between BMI and gene expression of anti-Müllerian hormone and androgen receptor in human granulosa cells in women with and without polycystic ovary syndrome.
abstract: BACKGROUND
  Anti-Müllerian hormone (AMH) is one of the most reliable markers of ovarian reserve. There is evidence which suggests that BMI may be associated with gene expression of AMH, AMH type II receptor (AMHR-II) and androgen receptor (AR) in human granulosa cells (GC) in women with and without polycystic ovary syndrome (PCOS).
  
  
  OBJECTIVE
  To investigate the association between BMI and gene expression of AMH, AMHR-II and AR in human GC in women with and without PCOS.
  
  
  METHODS
  In a cross-sectional study, hormonal profiles were measured among 38 patients with PCOS and 38 subjects without PCOS aged 18-40. AMH, AMHR-II and AR mRNA levels were quantified in cumulus GC. Pearson correlation and multiple linear regressions were used to assess the relationships.
  
  
  RESULTS
  Quantitative RT-PCR demonstrated that AMH and AMHR-II expression were negatively correlated with BMI (r = -0·39, P < 0·001 for AMH and r = -0·49, P < 0·001 for AMHR-II), whereas AR expression was positively correlated with BMI (r = 0·46, P < 0·001).
  
  
  CONCLUSIONS
  There is a negative association between AMH, AMHR-II expression and BMI, and a positive association between AR expression and BMI in the GC of PCOS and non-PCOS women.
authors.affiliation: Women's Reproductive Health Research Center, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nouri
authors.affiliation: Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran. aghadavod_m@yahoo.com.
authors.forename: Esmat
authors.initials: E
authors.lastname: Aghadavod
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz, Iran.
authors.forename: Sajad
authors.initials: S
authors.lastname: Khani
authors.affiliation: Endocrinology and Metabolism Research Center, Department of Gynecology and Obstetrics, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
authors.forename: Mehri
authors.initials: M
authors.lastname: Jamilian
authors.affiliation: Department of Gynecology and Obstetrics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Mehrnush
authors.initials: M
authors.lastname: Amiri Siavashani
authors.affiliation: Department of Gynecology and Obstetrics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.|Department of Gynecology and Obstetrics, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Ahmadi
authors.affiliation: Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran. asemi_r@yahoo.com.
authors.forename: Zatollah
authors.initials: Z
authors.lastname: Asemi
mesh_terms: D000293:Adolescent; D000328:Adult; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000378:metabolism*; D015992:Body Mass Index; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: C410798:AR protein, human; D011944:Receptors, Androgen; D054304:Anti-Mullerian Hormone