title: Superparamagnetic iron oxide nanoparticle targeting of adipose tissue-derived stem cells in diabetes-associated erectile dysfunction.
abstract: Erectile dysfunction (ED) is a major complication of diabetes, and many diabetic men with ED are refractory to common ED therapies. Adipose tissue-derived stem cells (ADSCs) have been shown to improve erectile function in diabetic animal models. However, inadequate cell homing to damaged sites has limited their efficacy. Therefore, we explored the effect of ADSCs labeled with superparamagnetic iron oxide nanoparticles (SPIONs) on improving the erectile function of streptozotocin-induced diabetic rats with an external magnetic field. We found that SPIONs effectively incorporated into ADSCs and did not exert any negative effects on stem cell properties. Magnetic targeting of ADSCs contributed to long-term cell retention in the corpus cavernosum and improved the erectile function of diabetic rats compared with ADSC injection alone. In addition, the paracrine effect of ADSCs appeared to play the major role in functional and structural recovery. Accordingly, magnetic field-guided ADSC therapy is an effective approach for diabetes-associated ED therapy.
authors.affiliation: Department of Andrology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Lei-Lei
authors.initials: LL
authors.lastname: Zhu
authors.affiliation: Department of Andrology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Andrology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: He-Song
authors.initials: HS
authors.lastname: Jiang
authors.affiliation: Department of Andrology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Andrology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Andrology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Yu-Tian
authors.initials: YT
authors.lastname: Dai
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D048909:Diabetes Complications / Q000188:drug therapy*; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D016503:Drug Delivery Systems; D007172:Erectile Dysfunction / Q000188:drug therapy* / Q000209:etiology*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D008297:Male; D010410:Penile Erection; D010413:Penis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D058185:Magnetite Nanoparticles; D042461:Vascular Endothelial Growth Factor A