title: Aliphatic glucosinolate synthesis and gene expression changes in gamma-irradiated cabbage.
abstract: Glucosinolates, found principally in the plant order Brassicales, are modulated by different post-harvest processing operations. Among these, ionizing radiation, a non-thermal process, has gained considerable interest for ensuring food security and safety. In gamma-irradiated cabbage, enhanced sinigrin, a major glucosinolate, has been reported. However, the molecular basis of such a radiation induced effect is not known. Herein, the effect of radiation processing on the expression of glucosinolate biosynthetic genes was investigated. RT-PCR based expression analysis of seven glucosinolate biosynthetic pathway genes (MYB28, CYP79F1, CYP83A1, SUR1, UGT74B1, SOT18 and TGG1) showed that CYP83A1, MYB28, UGT74B1, CYP79F1 and SUR1 were up-regulated in irradiated cabbage. The content of jasmonates, signalling molecules involved in glucosinolate induction was, however, unaffected in irradiated cabbage suggesting their non-involvement in glucosinolate induction during radiation processing. This is the first report on the effect of gamma irradiation on the expression of glucosinolate biosynthetic genes in vegetables.
authors.affiliation: Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Aparajita
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Archana N
authors.initials: AN
authors.lastname: Rai
authors.affiliation: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Suprasanna
authors.initials: S
authors.lastname: Penna
authors.affiliation: Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400085, India. Electronic address: prasadpsv@rediffmail.com.
authors.forename: Prasad S
authors.initials: PS
authors.lastname: Variyar
mesh_terms: D053898:Biosynthetic Pathways / Q000528:radiation effects*; D001937:Brassica / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D005961:Glucosinolates / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D005961:Glucosinolates; D010940:Plant Proteins
keywords: Brassica oleracea; Gene expression; Glucosinolates; Jasmonates