title: In VivoPrecision of Digital Topological Skeletonization Based Individual Trabecula Segmentation (ITS) Analysis of Trabecular Microstructure at the Distal Radius and Tibia by HR-pQCT.
abstract: Trabecular plate and rod microstructure plays a dominant role in the apparent mechanical properties of trabecular bone. With high-resolution computed tomography (CT) images, digital topological analysis (DTA) including skeletonization and topological classification was applied to transform the trabecular three-dimensional (3D) network into surface and curve skeletons. Using the DTA-based topological analysis and a new reconstruction/recovery scheme, individual trabecula segmentation (ITS) was developed to segment individual trabecular plates and rods and quantify the trabecular plate- and rod-related morphological parameters. High-resolution peripheral quantitative computed tomography (HR-pQCT) is an emergingin vivoimaging technique to visualize 3D bone microstructure. Based on HR-pQCT images, ITS was applied to various HR-pQCT datasets to examine trabecular plate- and rod-related microstructure and has demonstrated great potential in cross-sectional and longitudinal clinical applications. However, the reproducibility of ITS has not been fully determined. The aim of the current study is to quantify the precision errors of ITS plate-rod microstructural parameters. In addition, we utilized three different frequently used contour techniques to separate trabecular and cortical bone and to evaluate their effect on ITS measurements. Overall, good reproducibility was found for the standard HR-pQCT parameters with precision errors for volumetric BMD and bone size between 0.2%-2.0%, and trabecular bone microstructure between 4.9%-6.7% at the radius and tibia. High reproducibility was also achieved for ITS measurements using all three different contour techniques. For example, using automatic contour technology, low precision errors were found for plate and rod trabecular number (pTb.N, rTb.N, 0.9% and 3.6%), plate and rod trabecular thickness (pTb.Th, rTb.Th, 0.6% and 1.7%), plate trabecular surface (pTb.S, 3.4%), rod trabecular length (rTb.ℓ, 0.8%), and plate-plate junction density (P-P Junc.D, 2.3%) at the tibia. The precision errors at the radius were similar to those at the tibia. In addition, precision errors were affected by the contour technique. At the tibia, precision error by the manual contour method was significantly different from automatic and standard contour methods for pTb.N, rTb.N and rTb.Th. Precision error using the manual contour method was also significantly different from the standard contour method for rod trabecular number (rTb.N), rod trabecular thickness (rTb.Th), rod-rod and plate-rod junction densities (R-R Junc.D and P-R Junc.D) at the tibia. At the radius, the precision error was similar between the three different contour methods. Image quality was also found to significantly affect the ITS reproducibility. We concluded that ITS parameters are highly reproducible, giving assurance that future cross-sectional and longitudinal clinical HR-pQCT studies are feasible in the context of limited sample sizes.
authors.affiliation: Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, U.S.A.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, U.S.A.; Department of Orthopedic Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, U.S.A.
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.affiliation: Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, U.S.A.
authors.forename: Y Eric
authors.initials: YE
authors.lastname: Yu
authors.affiliation: Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, U.S.A.; McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA, U.S.A.
authors.forename: Xiaowei Sherry
authors.initials: XS
authors.lastname: Liu
authors.affiliation: Division of Endocrinology, Department of Medicine, Columbia University, New York, New York, U.S.A.
authors.forename: Kyle K
authors.initials: KK
authors.lastname: Nishiyama
authors.affiliation: Division of Endocrinology, Department of Medicine, Columbia University, New York, New York, U.S.A.
authors.forename: Mishaela R
authors.initials: MR
authors.lastname: Rubin
authors.affiliation: Division of Endocrinology, Department of Medicine, Columbia University, New York, New York, U.S.A.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Shane
authors.affiliation: Division of Endocrinology, Department of Medicine, Columbia University, New York, New York, U.S.A.
authors.forename: John P
authors.initials: JP
authors.lastname: Bilezikian
authors.affiliation: Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, U.S.A.
authors.forename: X Edward
authors.initials: XE
authors.lastname: Guo
keywords: HR-pQCT; Individual trabecular segmentation (ITS); osteoporosis; precision error; trabecular microstructure