title: Multivalent ligand mimetics of LecA from P. aeruginosa: synthesis and NMR studies.
abstract: Molecular recognition of glycans plays an important role in glycomic and glycobiology studies. For example, pathogens have a number of different types of lectin for targeting host sugars. In bacteria, lectins exist sometimes as domains of bacterial toxins and exploit adhesion to glycoconjugates as a means of entering host cells. Herein, we describe the synthesis of three glycodendrons with the aim to dissect the fine structural details involved in the multivalent carbohydrate-protein interactions. LecA, from the pathogen Pseudomonas aeruginosa, has been used to characterize galactose dendrons interaction using one of the most widespread NMR technique for the elucidation of receptor-ligand binding in solution, the saturation transfer difference (STD) NMR. Furthermore, the effective hydrodynamic radius of each dendrimer recognized by LecA was estimated from the diffusion coefficients determined by pulsed-field-gradient stimulated echo (PFG-STE) NMR experiments.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za della Scienza 2, 20126 Milano, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Bini
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, I-80126, Naples, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Marchetti
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za della Scienza 2, 20126 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Russo
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, I-80126, Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, I-80126, Naples, Italy.
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za della Scienza 2, 20126 Milano, Italy. Electronic address: laura.cipolla@unimib.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Cipolla
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D001665:Binding Sites; D002240:Carbohydrate Sequence; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D004058:Diffusion; D005690:Galactose / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018716:Molecular Mimicry; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D050091:Dendrimers; D006001:Glycoconjugates; C102929:LecA protein, bacteria; D037102:Lectins; D008024:Ligands; D005690:Galactose
keywords: Dendrons; Lectins; Ligand mimetics; P. aeruginosa; PFG-STE NMR experiments; STD NMR