title: Effects of Certolizumab on Cerulein-Induced Acute Pancreatitis in Rats.
abstract: To evaluate the effects of certolizumab, a pegylated monoclonal antibody to tumor necrosis factor α (TNF-α), on experimentally induced acute pancreatitis.
  
  
  
  Healthy Wistar Albino male rats (n = 36) were randomly divided into 4 groups (9 rats in each group): group 1, control group; group 2, certolizumab group; group 3, cerulein group; and group 4, cerulein + certolizumab group. Acute edematous pancreatitis was induced via intraperitoneal injection of 80-μg/kg cerulein (20 μg/kg, 4 times at 1-hour intervals) in groups 3 and 4. Certolizumab (10 μg) was intraperitoneally administered in groups 2 and 4. Serum levels of amylase, lipase, TNF-α, and lactate dehydrogenase were evaluated. Histopathology and immunohistochemistry of the pancreatic tissue for assessing the activities of malondialdehyde, myeloperoxidase, TNF-α, and caspase-3 were also performed after 72 hours.
  
  
  
  Certolizumab treatment significantly decreased the serum levels of amylase, lipase, and lactate dehydrogenase. Histopathological edema, hemorrhage, parenchymal necrosis, and infiltration scores were also decreased, along with a decrease in malondialdehyde, myeloperoxidase, TNF-α, and caspase-3 activities.
  
  
  
  This study suggests that certolizumab is a beneficial treatment mode for reducing the severity of acute pancreatitis.
authors.affiliation: From the *Gastroenterology Clinic, Hakkari State Hospital, Hakkari, Turkey; †Department of General Surgery, Medical Faculty, and ‡Department of Rheumatology, Medical Faculty, Pamukkale University, Kinikli Campus, Denizli, Turkey; Departments of §Pathology, and ∥Internal Medicine, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Istiklal Yerleskesi, Burdur, Turkey; and ¶Department of Gastroenterology, Medical Faculty, Kinikli Campus, Pamukkale University, Denizli, Turkey.
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Kosekli
authors.forename: Ugur
authors.initials: U
authors.lastname: Sungurtekin
authors.forename: Veli
authors.initials: V
authors.lastname: Cobankara
authors.forename: Ozlem
authors.initials: O
authors.lastname: Ozmen
authors.forename: Sima
authors.initials: S
authors.lastname: Sahinduran
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yilmaz
mesh_terms: D000208:Acute Disease; D000681:Amylases; D000818:Animals; D053148:Caspase 3; D002108:Ceruletide; D008297:Male; D010195:Pancreatitis; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha
chemical_list: D014409:Tumor Necrosis Factor-alpha; D002108:Ceruletide; D000681:Amylases; D053148:Caspase 3