title: Polydatin promotes apoptosis through upregulation the ratio of Bax/Bcl-2 and inhibits proliferation by attenuating the β-catenin signaling in human osteosarcoma cells.
abstract: Osteosarcoma is the most prevalent primary malignant bone tumor mainly endangering young adults. In this study, we explore whether polydatin (PD), a glycoside form of resveratrol, is effective for osteosarcoma. Our results showed that PD dose-dependently inhibited proliferation and promoted apoptosis in 143B and MG63 osteosarcoma cells, examined by MTT assay and Annexin V-FITC apoptosis detection. Further, we found PD increased expression of Bax and attenuated expression of Bcl-2, and consequently augmented caspase-3 activity. Moreover, PD also dose-dependently inhibited β-catenin signaling pathway as indicated by decreased β-catenin expression and activity, while overexpression of β-catenin by adenoviruses system could abrogate the anti-tumor effect of PD. Our finding indicated that PD could inhibit the proliferation by inhibiting the β-catenin signaling and induce apoptosis via upregulation the ratio of Bax/Bcl-2 in human osteosarcoma cells.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University Chongqing 400016, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Xu
authors.affiliation: Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University Chongqing 400016, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Kuang
authors.affiliation: Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University Chongqing 400016, China.
authors.forename: Wengao
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University Chongqing 400016, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University Chongqing 400016, China.
authors.forename: Dianming
authors.initials: D
authors.lastname: Jiang
keywords: Osteosarcoma; apoptosis; polydatin (PD); proliferation; β-catenin