title: Fabrication of poly(vinyl alcohol)-Carrageenan scaffolds for cryopreservation: Effect of composition on cell viability.
abstract: The present investigation reports the fabrication of three dimensional (3D), interconnected, highly porous, biodegradable scaffolds using freeze-gelation technique. The hydrogels prepared with different ratios (5:5, 6:4, 7:3, 8:2 and 9:1) of poly(vinyl alcohol) (PVA) and Carrageenan (Car) was lyophilized to obtain their respective scaffolds. The PVA-Car scaffolds were further characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The prepared scaffolds were found to be biodegradable and highly compatible with hemoglobin. Further, normal keratinocyte (HaCaT) and osteosarcoma (Saos-2) cells seeded on PVA-Car scaffolds were cryopreserved for 15days and their viability was checked at regular interval of 3days (0, 3, 6, 9, 12, 15 days) through MTT assay and fluorescence microscopy. Overall, the collective results indicate the scaffold constructs with 7:3 and 8:2 PVA-Car ratios possess ideal characteristics for tissue engineering applications and for long term cryopreservation of cells.
authors.affiliation: Department of Biotechnology, Thapar University, Patiala, Punjab, 147004, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Chopra
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Nayak
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Nanda
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India.
authors.forename: Sarbani
authors.initials: S
authors.lastname: Ashe
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India.
authors.forename: Pradipta Ranjan
authors.initials: PR
authors.lastname: Rauta
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India. Electronic address: nayakb@nitrkl.ac.in.
authors.forename: Bismita
authors.initials: B
authors.lastname: Nayak
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002351:Carrageenan / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D002351:Carrageenan; D011142:Polyvinyl Alcohol
keywords: Biocompatible; Biodegradable; Cell attachment; Cryopreservation; Scaffolds