title: Recycling of lipid-extracted hydrolysate as nitrogen supplementation for production of thraustochytrid biomass.
abstract: Efficient resource usage is important for cost-effective microalgae production, where the incorporation of waste streams and recycled water into the process has great potential. This study builds upon emerging research on nutrient recycling in thraustochytrid production, where waste streams are recovered after lipid extraction and recycled into future cultures. This research investigates the nitrogen flux of recycled hydrolysate derived from enzymatic lipid extraction of thraustochytrid biomass. Results indicated the proteinaceous content of the recycled hydrolysate can offset the need to supply fresh nitrogen in a secondary culture, without detrimental impact upon the produced biomass. The treatment employing the recycled hydrolysate with no nitrogen addition accumulated 14.86 g L(-1) of biomass in 141 h with 43.3 % (w/w) lipid content compared to the control which had 9.26 g L(-1) and 46.9 % (w/w), respectively. This improved nutrient efficiency and wastewater recovery represents considerable potential for enhanced resource efficiency of commercial thraustochytrid production.
authors.affiliation: Department of Process Engineering and Applied Science, Faculty of Engineering, Dalhousie University, Halifax, NS, B3H 4R2, Canada. Joshua.Lowrey@Dal.ca.|Mara Renewables Corporation, 101 Research Drive, Dartmouth, NS, B2Y 4T6, Canada. Joshua.Lowrey@Dal.ca.
authors.forename: Joshua
authors.initials: J
authors.lastname: Lowrey
authors.affiliation: Department of Process Engineering and Applied Science, Faculty of Engineering, Dalhousie University, Halifax, NS, B3H 4R2, Canada.|Mara Renewables Corporation, 101 Research Drive, Dartmouth, NS, B2Y 4T6, Canada.
authors.forename: Roberto E
authors.initials: RE
authors.lastname: Armenta
authors.affiliation: Department of Process Engineering and Applied Science, Faculty of Engineering, Dalhousie University, Halifax, NS, B3H 4R2, Canada.
authors.forename: Marianne S
authors.initials: MS
authors.lastname: Brooks
mesh_terms: D018533:Biomass; D008055:Lipids / Q000302:isolation & purification; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D059027:Recycling / Q000379:methods; D058009:Stramenopiles / Q000254:growth & development / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry
chemical_list: D008055:Lipids; D009584:Nitrogen; D062065:Wastewater
keywords: Amino acids; Enzyme hydrolysate; Heterotrophic microalgae; Nitrogen recycling; Nutrient recycling; Thraustochytrids