title: Molecular characterization and expression analysis of the β-actin gene from the ridgetail white prawn Exopalaemon carinicauda.
abstract: Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was 1335 bp long, comprising a 1131-bp ORF encoding 376 amino acids, a 65-bp 5'-UTR, and a 139-bp 3'-UTR with a poly(A) tail. The A + T content was approximately 79% in the 3'-UTR of the Ecβ-actin mRNA. The 3'-UTR contained two repeats of the AUUUA motif. The putative protein Ecβ-actin showed high identity (97-99%) with other actins from various species. Phylogenetic analysis revealed that Ecβ-actin belongs to Crustacea, although it formed a singleton sub-branch that was located a short distance from crabs and other shrimp species. Ecβ- actin expression was detected in the hepatopancreas, ovary, muscle, gill, stomach, and hemocytes, and was strongly expressed in the hemocytes and ovary of E. carinicauda. Ecβ-actin mRNA expression varied during ovarian development, with high levels observed at stages I and V. Therefore, caution should be taken when using the Ecβ-actin gene as an endogenous control gene.
authors.affiliation: Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, Xinxiang, Henan, China.|Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
authors.forename: J P
authors.initials: JP
authors.lastname: Liang
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Ge
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
authors.forename: J T
authors.initials: JT
authors.lastname: Li
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, Xinxiang, Henan, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Nie
mesh_terms: D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D043143:Hepatopancreas / Q000378:metabolism; D033541:Palaemonidae / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000199:Actins; D012333:RNA, Messenger