title: Effect of Platelet Rich Plasma Combined with Chitosan Biodegradable Film on Full-Thickness Wound Healing in Rat Model.
abstract: OBJECTIVE
  To assess the effects of platelet rich plasma (PRP) with chitosan biodegradable film on full thickness wound healing in rat.
  
  
  METHODS
  This was an experimental study being performed in 2015 during a 4-month period. Twenty-four male white Wistar rats were divided into four groups of 12 rats each, randomly: Control group (SHAM) with creation of wounds and no treatment, PRP group with creation of wounds and application of one milliliter PRP, Chitosan group (CHIT) with dressing the wound with chitosan and CHIT/PRP group with application of one mL PRPand dressing the wound with chitosan. The wounds were created by cutting healthy skin.Wound size was measured on 6, 9, 12, 15, 18 and 21 post surgery and was compared between groups.
  
  
  RESULTS
  Reduction in wound area, hydroxyproline contents and biomechanical parametersindicated there was significant difference (p=0.001) between group CHIT/PRP and other groups. Biomechanical testing was performed on day 9 post surgery in incisional model. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was significant difference (p<0.001) between group CHIT/PRP and other groups.
  
  
  CONCLUSIONS
  PRP with chitosan have beneﬁcial effects on wounds repair and could be suggested for treating various types of wounds in animals and human being.
authors.affiliation: Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran  
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.affiliation: Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran  
authors.forename: Moein
authors.initials: M
authors.lastname: Mehrtash
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran  
authors.forename: Moeid
authors.initials: M
authors.lastname: Mehrtash
authors.affiliation: Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran  
authors.forename: Nava
authors.initials: N
authors.lastname: Hassani
authors.affiliation: Department of Mechanical Engineering of Biosystems, Faculty of Agriculture, Urmia University, Urmia, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Hassanpour
keywords: Chitosan; Full-thickness wound; Platelet rich plasma (PRP); Wound healing