title: Controlled production of polysaccharides-exploiting nutrient supply for levan and heteropolysaccharide formation in Paenibacillus sp.
abstract: Bacterial exopolysaccharides (EPSs) are promising sustainable alternatives to synthetic polymers. Here we describe the production and characterization of different EPSs produced by the recently isolated Paenibacillus sp. 2H2. A final EPS titer of 4.54gL(-1) was recovered after a 17-h fermentation, corresponding to a volumetric productivity of 0.27gL(-1)h(-1). Remarkably, supplying the fermentation with specific carbon and nitrogen sources could be exploited for the production of different polymers. A pure heteropolysaccharide composed of glucose, mannose, galactose, and glucuronic acid (3.5:2:1:0.1) was obtained when using glucose/glycerol and peptone as substrates. A pure levan-type polymer or mixture of both polymers was observed with sucrose and NaNO3 or peptone. To our knowledge, this is the first report that nutrients, particularly nitrogen sources, can be used to fine-tune EPS production in Paenibacillaceae. Rheological characterization of the heteropolysaccharide revealed impressive thickening properties, suggesting its potential application in commodity materials.
authors.affiliation: Chair of Chemistry of Biogenic Resources, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany. Electronic address: m.ruetering@tum.de.
authors.forename: Marius
authors.initials: M
authors.lastname: Rütering
authors.affiliation: Chair of Chemistry of Biogenic Resources, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany. Electronic address: j.schmid@tum.de.
authors.forename: Jochen
authors.initials: J
authors.lastname: Schmid
authors.affiliation: Chair of Chemistry of Biogenic Resources, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany. Electronic address: broder.ruehmann@tum.de.
authors.forename: Broder
authors.initials: B
authors.lastname: Rühmann
authors.affiliation: Evonik Nutrition and Care GmbH, Goldschmidtstraße 100, 45127 Essen, Germany. Electronic address: martin.schilling@evonik.com.
authors.forename: Martin
authors.initials: M
authors.lastname: Schilling
authors.affiliation: Chair of Chemistry of Biogenic Resources, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany. Electronic address: sieber@tum.de.
authors.forename: Volker
authors.initials: V
authors.lastname: Sieber
mesh_terms: D005285:Fermentation; D005630:Fructans / Q000378:metabolism; D007218:Industrial Microbiology; D056507:Paenibacillus / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
chemical_list: D005630:Fructans; D011135:Polysaccharides, Bacterial; D013395:Sucrose
keywords: Carbon source; Exopolysaccharide; Levan; Nitrogen source; Paenibacillus; Rheology