title: Biomimetic 3D Clusters Using Human Adipose Derived Mesenchymal Stem Cells and Breast Cancer Cells: A Study on Migration and Invasion of Breast Cancer Cells.
abstract: Invasion and metastasis of cancer directly related to human death have been associated with interactions among many different types of cells and three-dimensional (3D) tissue matrices. Precise mechanisms related to cancer invasion and metastasis still remain unknown due to their complexities. Development of tumor microenvironment (TME)-mimicking system could play a key role in understanding cancer environments and in elucidating the relating phenomena and their driving forces. Here we report a facile and novel platform of 3D cancer cell-clusters using human adipose-derived mesenchymal stem cells (hASCs) and breast cancer cells (MDA-MB-231) within a collagen gel matrix to show cancer invasion in the cell and extracellular matrix (ECM). Both clusters A (hASC only) and AC (hASC and MDA-MB-231) exhibited different behaviors and expressions of migration and invasion, as observed by the relating markers such as fibronectin, α-SMA, and CXCR4. hASCs showed a protrusive migration from a cluster center, whereas MDA-MB-231 spread out radially followed by hASC migration. Finally, the effect of matrix was further discussed by varying collagen gel densities. The new biomimetic system of 3D cancer clusters developed here has the potential to be utilized for research on migration and invasion of cancer cells in extracellular matrices.
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology , Seoul 02792, Republic of Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Park
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology , Seoul 02792, Republic of Korea.|Department of Biomedical Engineering, University of Science and Technology , Daejeon 34113, Republic of Korea.
authors.forename: Boa
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Biopharmaceutical Sciences, University of Illinois , Chicago, Illinois 60612, United States.|Department of Integrated OMICS for Biomedical Science and Underwood International College, Yonsei University , Seoul 03706, Republic of Korea.
authors.forename: Seungpyo
authors.initials: S
authors.lastname: Hong
authors.affiliation: Center for Biomaterials, Korea Institute of Science and Technology , Seoul 02792, Republic of Korea.|Department of Biomedical Engineering, University of Science and Technology , Daejeon 34113, Republic of Korea.
authors.forename: Sang Heon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University , Seoul 08826, Republic of Korea.|Advanced Institutes of Convergence Technology , Gyeonggi-do 16229, Republic of Korea.
authors.forename: Kangwon
authors.initials: K
authors.lastname: Lee
mesh_terms: D032701:Biomimetics / Q000379:methods; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000473:pathology; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D059016:Tumor Microenvironment / Q000502:physiology
chemical_list: D005353:Fibronectins; D003094:Collagen
keywords: cell cluster; collagen gel; invasion; migration; tumor microenvironment