title: Significant reduction in allergenicity of ovalbumin from chicken egg white following treatment with ascidian viscera N-acetylglucosaminidase.
abstract: Ovalbumin (OA) is the most abundant ingredient of chicken egg-white allergenic proteins. In the present study we investigated the possibility of reducing OA allergenicity by treatment with a natural protein exhibiting N-acetylglucosaminidase (NA) activity. Ascidian is cultivated as a food resource in northeast Asia. The ascidian viscera NA (AVNA) with almost no other exoglycosidases or proteolytic enzymes was isolated by applying size-exclusion chromatography to a protein precipitate of ascidian viscera. Intact OA was mixed with AVNA containing 0.2, 1.0, and 5.0 Units of NA. Anion-exchange chromatography was then used to isolate OA from AVNA-treated OA. The electrophoretic patterns and N-glycans of each isolated OA from AVNA-treated OA (iOA) were analyzed, and the terminal N-acetylglucosamines of iOA were selectively cleaved with no other degradation occurring. A competitive indirect enzyme-linked immunosorbent assay using rabbit anti-OA sera was performed to investigate the allergenicity of iOA, which was found to be significantly reduced depending on the increased NA activity compared to that of intact OA. These results indicate that OA allergenicity was reduced using a simple and mild treatment process with AVNA, and suggest that ascidian NA is an efficient natural protein for reducing the allergenicity of OA without requiring the use of harsh physical treatments or chemical conjugation.
authors.affiliation: Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Hye Seong
authors.initials: HS
authors.lastname: Hwang
authors.affiliation: Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Heajin
authors.initials: H
authors.lastname: Park
authors.affiliation: Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Jihye
authors.initials: J
authors.lastname: Kim
authors.affiliation: Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Jai Yeon
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea.
authors.forename: Young Kwang
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute, 516 Baekhyun-dong, Bundang-gu, Gyeonggi 463-746, South Korea.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Park
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute, 516 Baekhyun-dong, Bundang-gu, Gyeonggi 463-746, South Korea.
authors.forename: Hee-Don
authors.initials: HD
authors.lastname: Choi
authors.affiliation: Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, South Korea. Electronic address: hahyung@cau.ac.kr.
authors.forename: Ha Hyung
authors.initials: HH
authors.lastname: Kim
mesh_terms: D000118:Acetylglucosaminidase / Q000302:isolation & purification / Q000378:metabolism*; D000485:Allergens / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004529:Egg White / Q000032:analysis; D005512:Food Hypersensitivity / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D014561:Urochordata / Q000201:enzymology*; D014781:Viscera / Q000201:enzymology
chemical_list: D000485:Allergens; D010047:Ovalbumin; D000118:Acetylglucosaminidase
keywords: Allergenicity; Ascidian; N-acetylglucosamine; N-acetylglucosaminidase; Ovalbumin