title: The Use of Small-Angle Scattering for the Characterization of Multi Subunit Complexes.
abstract: As the continuing trend in structural biology is to probe ever more complex systems, new methodologies are being developed plus existing techniques are being expanded and adapted, to keep up with the demands of the research community. To investigate multi subunit complexes (protein-DNA, protein-RNA or protein-protein complexes) no one technique holds a monopoly, as each technique yields independent information inaccessible to the other methods, but can be used together in a complementary way. Additionally as large conformational changes are not unlikely, investigation of the dynamics of these systems under physiological conditions is needed to fully understand their function. Investigations under physiological conditions in solution are becoming more standardized and with more dedicated, automated beamlines available these experiments are easy to access by the general research community. As such the need for explanations of how to plan and undertake these experiments is needed. In this chapter we will cover the requirements of these experiments as well and how to plan undertake and analyze the results of such experiments.
authors.affiliation: European Molecular Biology Laboratory, Grenoble, 71 avenue des Martyrs, 38042, France. around@embl.fr.|Unit for Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, 71 avenue des Martyrs, 38042, Grenoble, France. around@embl.fr.|Joint Structural Biology Group, European Synchrotron Radiation Facility, Grenoble, 71 avenue des Martyrs, 38042, France. around@embl.fr.|Faculty of Natural Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK. around@embl.fr.
authors.forename: Adam
authors.initials: A
authors.lastname: Round
mesh_terms: D000818:Animals; D001665:Binding Sites; D019295:Computational Biology; D030562:Databases, Protein; D006801:Humans; D008958:Models, Molecular; D046912:Multiprotein Complexes; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053838:Scattering, Small Angle; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D011994:Recombinant Proteins
keywords: BioSAS; Complementary techniques; Contrast; Neutrons; SAS; SEC; Solution scattering; X-rays