title: Temporal regulation of extracellular signal-regulated kinase 1/2 phosphorylation, heat shock protein 70 and activating transcription factor 3 during prostaglandin F-induced luteal regression in pseudopregnant rats following heat stress.
abstract: The aim of the present study was to investigate the effects of heat stress on heat shock protein (HSP) 70 expression and mitogen-activated protein kinase (MAPK) and protein kinase (PK) B signalling during prostaglandin F (PGF)-induced luteal regression. During pseudopregnancy, rats were exposed to heat stress (HS, 40°C, 2h) for 7 days and treated with PGF or physiological saline on Day 7; serum and ovaries were collected 0, 1, 2, 8 or 24h after PGF treatment. The early inhibitory effect of PGF on progesterone was reduced in HS rats. HSP70 expression in response to PGF was significantly enhanced in HS rats. PGF-induced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was significantly greater in the HS group; however, HS rats exhibited elevated basal levels of phosphorylation of p38 MAPK, but not ERK1/2. PGF treatment increased expression of activating transcription factor (ATF) 3 at 2h, which was inhibited by heat stress. Evaluating PKB signalling revealed that phosphorylation of p-Akt (Thr308and Ser473) was reduced at 8 and 24h after PGF treatment in both non-heat stress (NHS) and HS groups, but there were no significant differences between the HS and NHS groups at any of the time points. In conclusion, the present study provides further evidence that heat stress may enhance HSP70 and affect ERK1/2 and ATF3 expression, but not Akt activation, during PGF-induced luteal regression in pseudopregnant rats.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Wu-Jiao
authors.initials: WJ
authors.lastname: Bai
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Peng-Jing
authors.initials: PJ
authors.lastname: Jin
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Mei-Qian
authors.initials: MQ
authors.lastname: Kuang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Quan-Wei
authors.initials: QW
authors.lastname: Wei
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Fang-Xiong
authors.initials: FX
authors.lastname: Shi
authors.affiliation: VA Nebraska-Western Iowa Health Care System and Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: John S
authors.initials: JS
authors.lastname: Davis
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Da-Gan
authors.initials: DG
authors.lastname: Mao
mesh_terms: D051700:Activating Transcription Factor 3 / Q000378:metabolism*; D000818:Animals; D003008:Cloprostenol / Q000494:pharmacology; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018882:Heat Stress Disorders / Q000097:blood / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007700:Kinetics; D003341:Luteolysis / Q000097:blood / Q000187:drug effects / Q000378:metabolism*; D008186:Luteolytic Agents / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation / Q000187:drug effects; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000097:blood; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011555:Pseudopregnancy / Q000150:complications*; D017207:Rats, Sprague-Dawley; D013047:Specific Pathogen-Free Organisms; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D051700:Activating Transcription Factor 3; C497108:Atf3 protein, rat; D018840:HSP70 Heat-Shock Proteins; D008186:Luteolytic Agents; D003008:Cloprostenol; D011374:Progesterone; C494920:Akt1 protein, rat; D051057:Proto-Oncogene Proteins c-akt; C535149:Mapk1 protein, rat; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases