title: Amelioration of Hyperglycaemia, Oxidative Stress and Dyslipidaemia in Alloxan-Induced Diabetic Wistar Rats Treated with Probiotic and Vitamin C.
abstract: Clinical and experimental evidence suggests that hyperglycaemia is responsible for the oxidative stress in diabetes mellitus. The study was designed to investigate the comparative effects of probiotic and vitamin C (Vit-C) treatments on hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic rats. Type 1 diabetes (T1DM) was induced in male Wistar rats by a single intraperitoneal (i.p.) injection of alloxan (150 mg/kg). Six groups of the animals received the following treatment regimens for four weeks: (1) Normal saline, per os; (2) alloxan (150 mg/kg, i.p.); (3) alloxan (150 mg/kg) + insulin (4 U/kg, subcutaneously); (4) alloxan (150 mg/kg) + probiotic (4.125 × 10⁶ CFU/100 mL per os); (5) alloxan (150 mg/kg) + Vit-C (100 mg/kg, i.m.); (6) alloxan (150 mg/kg) + probiotic (4.125 × 10⁶ CFU/100 mL per os) + Vit-C (100 mg/kg, intramuscularly). Probiotic + Vit-C decreased (p < 0.05) blood glucose concentration in diabetic treated group, when compared with the untreated diabetic group. Probiotic + Vit-C reduced malondialdehyde concentration, in the serum, brain and kidneys, respectively, but increased the activity of antioxidant enzymes. Probiotic and Vit-C may be more effective than Vit-C alone, in ameliorating hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic rats.
authors.affiliation: Department of Veterinary Physiology, Ahmadu Bello University, c/o P.O. Box 216 Samaru, 810006 Zaria, Nigeria. aluwong_tagang@yahoo.co.UK.
authors.forename: Tagang
authors.initials: T
authors.lastname: Aluwong
authors.affiliation: Department of Veterinary Physiology, Ahmadu Bello University, c/o P.O. Box 216 Samaru, 810006 Zaria, Nigeria. ayojo94@yahoo.com.
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Ayo
authors.affiliation: Department of Veterinary Physiology, Ahmadu Bello University, c/o P.O. Box 216 Samaru, 810006 Zaria, Nigeria. alkalivet@gmail.com.
authors.forename: Alkali
authors.initials: A
authors.lastname: Kpukple
authors.affiliation: Biochemistry Division, National Veterinary Research Institute, 930103 Vom, Nigeria. oladiposola@ymail.com.
authors.forename: Olusola Olalekan
authors.initials: OO
authors.lastname: Oladipo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D001786:Blood Glucose; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D050171:Dyslipidemias / Q000188:drug therapy*; D006943:Hyperglycemia / Q000188:drug therapy*; D007328:Insulin / Q000627:therapeutic use; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde; D018384:Oxidative Stress / Q000187:drug effects*; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D008315:Malondialdehyde; D001205:Ascorbic Acid
keywords: dyslipidaemia; hyperglycaemia; oxidative stress; type 1 diabetes; wistar rats