title: Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes.
abstract: We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We found that the precision of the two libraries in detecting essential genes was similar and that combining data from both screens improved performance. Notably, results from the two screens showed little correlation, which can be partially explained by the identification of distinct essential biological processes with each technology.
authors.affiliation: Department of Genetics, Stanford University, Stanford, California, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Morgens
authors.affiliation: Department of Genetics, Stanford University, Stanford, California, USA.|Department of Chemistry, Stanford University, Stanford, California, USA.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Deans
authors.affiliation: Department of Genetics, Stanford University, Stanford, California, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Genetics, Stanford University, Stanford, California, USA.|Stanford University Chemistry, Engineering, and Medicine for Human Health (ChEM-H), Stanford, California, USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Bassik
mesh_terms: D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D020043:Genes, Essential / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D034741:RNA, Small Interfering / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D064130:CRISPR-Associated Proteins; D034741:RNA, Small Interfering