title: [The effect of phospholipid transfer protein on cigarette smoke extract induced epithelial-mesenchymal transition of rat alveolar type Ⅱ cells].
abstract: OBJECTIVE
  To investigate the effect of phospholipid transfer protein(PLTP) on cigarette smoke extract(CSE) induced epithelial-mesenchymal transition(EMT) in rat alveolar Type Ⅱ cells (RLE-6TN).
  
  
  METHODS
  CSE of different concentrations (0%, 0.25%, 0.5% and 1%) was co-cultured for 2 or 3 days with RLE-6TN, either pre-treated or not pre-treated with siRNA-PLTP for 6 h. Expression levels of E-cadherin mRNA and Vimentin mRNA were examined by RT-PCR, while expression levels of PLTP, E-cadherin, N-cadherin and Vimentin were examined by Western blot.
  
  
  RESULTS
  Our results showed that the expression of E-cadherin mRNA decreased in CSE-treated groups: 1.01±0.05, 0.74±0.05, 0.65±0.03, 0.30±0.08 respectively at different concentrations of CSE (0 %, 0.25%, 0.5 %, and 1.0%); while the level of Vimentin mRNA increased significantly in 1% CSE treated cells(1.88±0.49), compared with control cells (1.01±0.20). Treatment with CSE at different concentrations (0%, 0.25%, 0.5% and 1%) showed that the protein levels of PLTP were 0.42±0.02, 0.89±0.25, 1.08±0.18, 1.61±0.06 respectively; those of E-cadherin were 1.61±0.04, 1.08±0.10, 0.62±0.08, 0.68±0.17, respectively; those of N-cadherin were 0.60±0.14, 0.57±0.26, 0.88±0.30, 1.94±0.54, respectively; and those of Vimentin were 0.61±0.05, 0.98±0.16, 1.07±0.14, 1.34±0.19, respectively; all P<0.05 when the 1% CSE group was compared with the control group. EMT induced by CSE was significantly inhibited by siRNA-PLTP.
  
  
  CONCLUSIONS
  PLTP may be involved in CSE induced EMT of rat alveolar cells.
authors.affiliation: Department of Respiratory Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: F P
authors.initials: FP
authors.lastname: Wu
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chen
authors.forename: X Y
authors.initials: XY
authors.lastname: Yu
authors.forename: K
authors.initials: K
authors.lastname: Liao
authors.forename: D D
authors.initials: DD
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Chen
mesh_terms: D056809:Alveolar Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002478:Cells, Cultured; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D049294:Phospholipid Transfer Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; D049294:Phospholipid Transfer Proteins; D034741:RNA, Small Interfering; D012906:Smoke; D014746:Vimentin