title: Conjugated linoleic acid and nitrite attenuate mitochondrial dysfunction during myocardial ischemia.
abstract: Cardiovascular health is influenced by dietary composition and the western diet is composed of varying types/amounts of fat. Conjugated linoleic acid (cLA) is an abundant dietary unsaturated fatty acid associated with health benefits but its biological signaling is not well understood. Nitrite is enriched in vegetables within the diet and can impact signaling of unsaturated fatty acids; however, its role on cLA signaling is not well understood. Elucidating how nitrite may impact the biological signaling of cLA is important due to the dietary consumption of both cLA and nitrite in the western diet. Since co-administration of cLA and nitrite results in cardioprotection during myocardial infarction (MI), it was hypothesized that cLA and nitrite may affect cardiac mitochondrial respiratory function and complex activity in MI. C57BL/6J mice were treated with cLA and nitrite for either 10 or 13days, where MI was induced on day 3. Following treatment, respiration and complex activity were measured. Among the major findings of this study, cLA treatment (10days) decreases state 3 respiration in vivo. Following MI, nitrite alone and in combination with cLA attenuates increased state 3 respiration and decreases hydrogen peroxide levels. Further, nitrite and cLA co-treatment attenuates increased complex III activity after MI. These results suggest that cLA, nitrite and the combination significantly alter cardiac mitochondrial respiratory and electron transport chain activity in vivo and following MI. Overall, the daily consumption of cLA and nitrite in the diet can have diverse cardiovascular implications, some of which occur at the mitochondrial level.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY, 40202, USA.
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Van Hoose
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY, 40202, USA.
authors.forename: Natia Qipshidze
authors.initials: NQ
authors.lastname: Kelm
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY, 40202, USA.
authors.forename: Kellianne M
authors.initials: KM
authors.lastname: Piell
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY, 40202, USA. Electronic address: marcie.cole@louisville.edu.
authors.forename: Marsha P
authors.initials: MP
authors.lastname: Cole
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004452:Echocardiography; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000378:metabolism; D042963:Electron Transport Complex II / Q000378:metabolism; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D006321:Heart / Q000000981:diagnostic imaging; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D008810:Mice, Inbred C57BL; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism*; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000000981:diagnostic imaging / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D010086:Oxidative Phosphorylation Coupling Factors / Q000378:metabolism; D018384:Oxidative Stress; D012977:Sodium Nitrite / Q000627:therapeutic use*
chemical_list: D002316:Cardiotonic Agents; D044243:Linoleic Acids, Conjugated; D010086:Oxidative Phosphorylation Coupling Factors; C046938:9,11-linoleic acid; D006861:Hydrogen Peroxide; D042963:Electron Transport Complex II; D003576:Electron Transport Complex IV; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III; D012977:Sodium Nitrite
keywords: Cardiovascular health; Conjugated linoleic acid; Diet; Mitochondria; Myocardial infarction; Nitrite