title: Identification of mitochondrial DNA transfer in canine transmissible venereal tumours obtained from dogs in Mexico.
abstract: Canine transmissible venereal tumour (CTVT) has been transmitted by cell transplantation from dog to dog, for over 10 000 years. Although initial studies report a single genetic origin for CTVT, recent samples from around the world reveal high genetic diversity. An elevated number of polymorphisms have been determined in mitochondrial DNA (mtDNA) of CTVT. The recent discovery of mtDNA transference from the host into tumoural cells could be a novel source of genetic diversity in CTVT. The aim of this study was to determine the presence of host mtDNA in samples of CTVT in Mexican dogs. Genotyping of 49 samples of CTVT and 49 samples of blood cells pertaining to affected dogs was performed by direct sequencing from the mtDNA D-loop region. Exogenous mtDNA was observed in 6% of the analysed tumours. This is the first investigation reporting the prevalence of exogenous mtDNA in CTVT in the Mexican dog population.
authors.affiliation: a Universidad Autónoma del Estado de México, Centro Universitario Amecameca, Carretera Amecameca-Ayapango , Amecameca , Estado de México , México.
authors.forename: Linda
authors.initials: L
authors.lastname: Bautista-Gómez
authors.affiliation: b Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia , Universidad Autónoma del Estado de México, Carretera de Cuota Toluca-Atlacomulco , Toluca , Estado de México , México.
authors.forename: Simón
authors.initials: S
authors.lastname: Martínez-Castañeda
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004283:Dog Diseases / Q000235:genetics / Q000635:transmission*; D004285:Dogs; D005838:Genotype; D008800:Mexico; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014685:Venereal Tumors, Veterinary / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial
keywords: CTVT; Cancer; dog; mitochondrial transfer