title: Thalassospira australica sp. nov. isolated from sea water.
abstract: Two Gram-negative, non-pigmented, motile bacteria were isolated from a sea water sample collected at St. Kilda Beach, Port Philip Bay, Victoria, Australia. The two strains were found to grow between 4 and 40 °C, pH 5-10 and tolerate up to 10 % NaCl. A phylogenetic study, based on a 16S rRNA gene sequence analysis indicated that strains NP 3b2(T) and H 94 belong to the genus Thalassospira. The sequence similarity of the 16S rRNA gene between the two new isolates is 99.8 % and between these strains and all validly named Thalassospira species was found to be in the range of 95-99.4 %. The DNA-DNA relatedness between the two strains was found to be 80.2 %, while relatedness with other validly named species of the genus Thalassospira was between 53 and 65 %. The average nucleotide identity (ANI) and the in silico genome-to-genome distance (GGD) between the two bacteria and T. profundimaris WP0211(T), T. xiamenensis M-5(T), 'T. permensis' NBRC 106175(T) and T. lucentensis QMT2(T) was 76-82 % and 21-25 %, respectively. The results of phylogenetic and genomic analysis, together with physiological and biochemical properties, indicated that the two strains represent a new species of the genus Thalassospira. Based on these data, a new species, Thalassospira australica, is proposed with strain NP 3b2(T) (=KMM 6365(T) = JCM 31222(T)) as the type strain.
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia. eivanova@swin.edu.au.
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.affiliation: Universidad Miguel Hernandez, Apartado 18, 03550, San Juan de Alicante, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: López-Pérez
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia.
authors.forename: Hayden K
authors.initials: HK
authors.lastname: Webb
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia.
authors.forename: Hooi Jun
authors.initials: HJ
authors.lastname: Ng
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia.
authors.forename: Thi Hoang Yen
authors.initials: TH
authors.lastname: Dang
authors.affiliation: A.V. Zhirmunsky Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, Pal'chevskogo Str., 17, Vladivostok, Russian Federation, 690041.|Far Eastern Federal University, Vladivostok, Russian Federation, 690950.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku, 159, Vladivostok, Russian Federation, 690022.|Far Eastern Federal University, 8 Suhanova St., Vladivostok, Russian Federation, 690950.
authors.forename: Valery V
authors.initials: VV
authors.lastname: Mikhailov
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia.
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Crawford
authors.affiliation: Universidad Miguel Hernandez, Apartado 18, 03550, San Juan de Alicante, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodriguez-Valera
mesh_terms: D001315:Australia; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Genomic taxonomy; Marine bacteria; New species; Phylogeny; Taxonomy; Thalassospira