title: Exogenous α-Synuclein Monomers Alter Dopamine Metabolism in Murine Brain.
abstract: Alpha-synuclein (ASN) is a small presynaptic protein which is the major component of Lewy bodies-the histological hallmark of Parkinson's disease. Among many functions, ASN plays an important role in regulation of dopaminergic system by controlling dopamine concentration at nerve terminals. An abnormal structure or excessive accumulation of ASN in the brain can induce neurotoxicity leading to the dopaminergic neurodegeneration. To date, several transgenic mouse lines overexpressing ASN have been generated and there are several studies using injections of ASN fibrils into the murine brain. However, still is little known about the effects of exogenously applied ASN monomers on dopaminergic neurotransmission. In this study we investigated the influence of cerebral injection of human ASN on dopaminergic system activity. We have demonstrated that a single injection of ASN monomers into the substantia nigra pars compacta or striatum is sufficient to affect dopaminergic neurotransmission in murine nigro-striatal system.
authors.affiliation: Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology CePT, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland.
authors.forename: Adriana
authors.initials: A
authors.lastname: Wawer
authors.affiliation: Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology CePT, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland. ilona.joniec@wum.edu.pl.
authors.forename: Ilona
authors.initials: I
authors.lastname: Joniec-Maciejak
authors.affiliation: Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology CePT, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sznejder-Pachołek
authors.affiliation: Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology CePT, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Schwenkgrub
authors.affiliation: Department of Neurosurgery, University of California at San Francisco, San Francisco, CA, USA.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ciesielska
authors.affiliation: Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology CePT, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland.|2nd Department of Neurology, Institute of Psychiatry and Neurology, Warsaw, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Mirowska-Guzel
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D006801:Humans; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013378:Substantia Nigra / Q000187:drug effects* / Q000378:metabolism*; D051844:alpha-Synuclein / Q000008:administration & dosage*
chemical_list: D051844:alpha-Synuclein; D004298:Dopamine
keywords: Alpha-synuclein; Dopamine; Dopaminergic system; Parkinson’s disease; Tyrosine hydroxylase