title: Hippocampal distribution of IL-1β and IL-1RI following lithium-pilocarpine-induced status epilepticus in the developing rat.
abstract: The contribution of Interleukin-1β (IL-1β) to neuronal injury induced by status epilepticus (SE) in the immature brain remains unclear. The goal of this study was to determine the hippocampal expression of IL-1β and its type 1 receptor (IL-1RI) following SE induced by the lithium-pilocarpine model in fourteen-days-old rat pups; control animals were given an equal volume of saline instead of the convulsant. IL-1β and IL-1RI mRNA hippocampal levels were assessed by qRT-PCR 6 and 24 h after SE or control conditions. IL-1β and IL-1RI expression was detected in the dorsal hippocampus by immunohistochemical procedures; Fluoro-Jade B staining was carried out in parallel sections in order to detect neuronal cell death. IL-1β mRNA expression was increased 6 h following SE, but not at 24 h; however IL-1RI mRNA expression was unaffected when comparing with the control group. IL-1β and IL-1RI immunoreactivity was not detected in control animals. IL-1β and IL-1RI were expressed in the CA1 pyramidal layer, the dentate gyrus granular layer and the hilus 6 h after SE, whereas injured cells were detected 24 h following seizures. Early expression of IL-1β and IL-1RI in the hippocampus could be associated with SE-induced neuronal cell death mechanisms in the developing rat.
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Unidad de Ciencias de la Salud, Médicos y Odontólogos, s/n, Col. Unidad del Bosque, C.P. 91010, Xalapa, Veracruz, México, Universidad Veracruzana, Universidad Veracruzana, Centro de Investigaciones Cerebrales, Xalapa Veracruz , México.|Doutorado em Neuroetologia, Universidad Veracruzana, Av. Luis Castelazo, s/n, Col. Industrial Ánimas, C.P. 91190, Xalapa, Veracruz, México, Universidad Veracruzana, Universidad Veracruzana, Xalapa Veracruz , México.
authors.forename: Dulce-Mariely
authors.initials: DM
authors.lastname: Álvarez-Croda
authors.affiliation: Instituto de Investigaciones Biológicas, Universidad Veracruzana, Av. Luis Castelazom s/n, Col. Industrial Ánimas, C.P. 91190, Xalapa, Veracruz, México, Universidad Veracruzana, Universidad Veracruzana, Instituto de Investigaciones Biológicas, Xalapa Veracruz , México.
authors.forename: Juan
authors.initials: J
authors.lastname: Santiago-García
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Unidad de Ciencias de la Salud, Médicos y Odontólogos, s/n, Col. Unidad del Bosque, C.P. 91010, Xalapa, Veracruz, México, Universidad Veracruzana, Universidad Veracruzana, Centro de Investigaciones Cerebrales, Xalapa Veracruz , México.
authors.forename: Jesús S
authors.initials: JS
authors.lastname: Medel-Matus
authors.affiliation: Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas-IPN, Wilfrido Massieu, s/n, Unidad Profesional Adolfo López Mateos, Gustavo A. Madero, C.P. 07738, Mexico D.F., México, Escuela Nacional de Ciencias Biológicas, Departamento de Farmacia, Mexico D.F. , México.
authors.forename: Joel
authors.initials: J
authors.lastname: Martínez-Quiroz
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Unidad de Ciencias de la Salud, Médicos y Odontólogos, s/n, Col. Unidad del Bosque, C.P. 91010, Xalapa, Veracruz, México, Universidad Veracruzana, Universidad Veracruzana, Centro de Investigaciones Cerebrales, Xalapa Veracruz , México.
authors.forename: Angel A
authors.initials: AA
authors.lastname: Puig-Lagunes
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Unidad de Ciencias de la Salud, Médicos y Odontólogos, s/n, Col. Unidad del Bosque, C.P. 91010, Xalapa, Veracruz, México, Universidad Veracruzana, Universidad Veracruzana, Centro de Investigaciones Cerebrales, Xalapa Veracruz , México.
authors.forename: Luis
authors.initials: L
authors.lastname: Beltrán-Parrazal
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Unidad de Ciencias de la Salud, Médicos y Odontólogos, s/n, Col. Unidad del Bosque, C.P. 91010, Xalapa, Veracruz, México, Universidad Veracruzana, Universidad Veracruzana, Centro de Investigaciones Cerebrales, Xalapa Veracruz , México.
authors.forename: María-Leonor
authors.initials: ML
authors.lastname: López-Meraz
mesh_terms: D000818:Animals; D003292:Convulsants; D004195:Disease Models, Animal; D006624:Hippocampus / Q000378:metabolism*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008094:Lithium; D010862:Pilocarpine; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism*
chemical_list: D003292:Convulsants; D053583:Interleukin-1beta; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D010862:Pilocarpine; D008094:Lithium