title: Noninvasive In Vivo Fluorescence Imaging of NK Cells in Preclinical Models of Adoptive Immunotherapy.
abstract: Preclinical animal models play a vital role in developing novel adoptive immunotherapies for cancer. In these in vivo models, it is essential to track the adoptively transferred cells to understand their tissue localization (biodistribution) in order to correlate with observed therapeutic outcomes as well as to develop novel approaches to promote homing to tumors or organs of interest. This chapter describes a simple and quick method for fluorescence labeling and in vivo imaging of adoptively transferred NK cells in small animal models.
authors.affiliation: Division of Pediatrics, The University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Blvd, Unit # 853, Houston, TX, 77054, USA. sssomanchi@mdanderson.org.
authors.forename: Srinivas S
authors.initials: SS
authors.lastname: Somanchi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D004195:Disease Models, Animal; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology / Q000637:transplantation*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D009928:Organ Specificity; D014018:Tissue Distribution
keywords: Adoptive immunotherapy; DiR; Fluorescence imaging; NK cells; Near infrared dye