title: Association of the Genetic Polymorphisms RRM1 -756T>C and -269C>A With Cervical Neoplasia.
abstract: Cervical neoplasia is one of the most prevalent malignant neoplasms worldwide. Ribonucleotide reductase 1 (RRM1) is thought to play an essential role in modulating the development and progression of cervical neoplasia. Two novel genetic polymorphisms, RRM1 -756T>C and -269 C>A, are significantly correlated with RRM1 expression. Some epidemiological studies have demonstrated that genetic variants play a crucial role in susceptibility to cervical cancer. The present study aimed to identify the genetic polymorphisms RRM1 -756T>C and -269 C>A in patients with cervical neoplasia and healthy controls. In total, 493 subjects, comprising 324 healthy controls and 169 patients with cervical neoplasia, were enrolled for this study. The allelic discrimination of the RRM1 -756T>C (rs11030918) and -269C>A (rs12806698) polymorphisms was assessed using the ABI StepOne™ real-time polymerase chain reaction system and analyzed using Software Design Specification (SDS), Version 3.0, software with TaqMan assays. The risk of cervical cancer was examined, revealing adjusted odds ratios and 95% confidence intervals of 1.25 [0.51, 3.08] and 1.09 [0.43, 2.78] for individuals with CC alleles of RRM1 -756T>C and for individuals with AA alleles of RRM1 -269C>A genetic polymorphisms, respectively, compared to individuals with wild-type RRM1 genetic polymorphisms. No significant genetic interaction effect was observed in susceptibility to cervical neoplasia, and no association was found between genetic polymorphisms and clinical statuses of invasive cervical cancer. The genetic polymorphisms RRM1 -756T>C and -269C>A may not be a factor for susceptibility to cervical neoplasia.
authors.affiliation: Department of Obstetrics and Gynecology, Taipei Medical University Hospital, Taipei, Taiwan School of Nursing, College of Nursing, Taipei Medical University, Taipei, Taiwan.
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Chang
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Po-Hui
authors.initials: PH
authors.lastname: Wang
authors.affiliation: School of Nursing, College of Nursing, Taipei Medical University, Taipei, Taiwan.
authors.forename: Hsiu-Ju
authors.initials: HJ
authors.lastname: Chang
authors.affiliation: Business Development Department, Taipei Medical University Hospital, Taipei, Taiwan Institute of Clinical Medical Sciences, Chang Gung University, Taipei, Taiwan.
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Liu
authors.affiliation: School of Nursing, College of Nursing, Taipei Medical University, Taipei, Taiwan Accelerated Bachelor of Science in Nursing, College of Nursing, Taipei Medical University, Taipei, Taiwan Department of Nursing, Taipei Medical University Hospital, Taipei, Taiwan tsaihsiuting@yahoo.com.tw hsiuting@tmu.edu.tw.
authors.forename: Hsiu-Ting
authors.initials: HT
authors.lastname: Tsai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012264:Ribonucleotide Reductases / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D025521:Tumor Suppressor Proteins; D012264:Ribonucleotide Reductases
keywords: RRM1 −269 C > A; RRM1 −756T>C; cervical neoplasia; ribonucleotide reductase 1 (RRM1); single nucleotide polymorphism (SNP)