title: Synthesis and Properties of Hemostatic and Bacteria-Responsive in Situ Hydrogels for Emergency Treatment in Critical Situations.
abstract: Immediate hemorrhage control and infection prevention are pivotal for saving lives in critical situations such as battlefields, natural disasters, traffic accidents, and so on. In situ hydrogels are promising candidates, but their mechanical strength is often not strong enough for use in critical situations. In this study, we constructed three hydrogels with different amounts of Schiff-base moieties from 4-arm-PEG-NH2, 4-arm-PEG-NHS, and 4-arm-PEG-CHO in which vancomycin was incorporated as an antimicrobial agent. The hydrogels possess porous structures, excellent mechanical strength, and high swelling ratio. The cytotoxicity studies indicated that the composite hydrogel systems possess good biocompatibility. The Schiff bases incorporated improve the adhesiveness and endow the hydrogels with bacteria-sensitivity. The in vivo hemostatic and antimicrobial experiments on rabbits and pigs demonstrated that the hydrogels are able to aid in rapid hemorrhage control and infection prevention. In summary, vancomycin-loaded hydrogels may be excellent candidates as hemostatic and antibacterial materials for first aid treatment of the wounded in critical situations.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics & Chemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Bu
authors.affiliation: Department of Orthopaedics, Chinese PLA General Hospital , Beijing 100853, China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Chinese PLA General Hospital , Beijing 100853, China.
authors.forename: Jianheng
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Chinese PLA General Hospital , Beijing 100853, China.
authors.forename: Lihai
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Chinese PLA General Hospital , Beijing 100853, China.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics & Chemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics & Chemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics & Chemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, Chinese PLA General Hospital , Beijing 100853, China.
authors.forename: Peifu
authors.initials: P
authors.lastname: Tang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics & Chemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
authors.forename: Decheng
authors.initials: D
authors.lastname: Wu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004638:Emergency Treatment; D006490:Hemostatics / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols; D011817:Rabbits; D012545:Schiff Bases / Q000737:chemistry; D013552:Swine; D014640:Vancomycin / Q000737:chemistry / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D006490:Hemostatics; D020100:Hydrogels; D012545:Schiff Bases; D011092:Polyethylene Glycols; D014640:Vancomycin
keywords: PEG; antibacteria; hemostasis; hydrogels; pH-responsive