title: Bioaugmentation strategy employing a microbial consortium immobilized in chitosan beads for oil degradation in mesocosm scale.
abstract: A bacterial consortium composed by four metagenomic clones and Bacillus subtilis strain CBMAI 707, all derived from petroleum reservoirs, was entrapped in chitosan beads and evaluated regarding hydrocarbon degradation capability. Experiments were carried out in mesocosm scale (3000L) with seawater artificially polluted with crude oil. At different time intervals, mesocosms were sampled and subjected to GC-FID and microbiological analyses, as total and heterotrophic culturable bacterial abundance (DAPI and CFU count), biological oxygen demand (BOD) and taxonomic diversity (massive sequencing of 16S rRNA genes). The results obtained showed that degradation of n-alkane hydrocarbons was similar between both treatments. However, aromatic compound degradation was more efficient in bioaugmentation treatment, with biodegradation percentages reaching up to 99% in 30days. Community dynamics was different between treatments and the consortium used in the bioaugmentation treatment contributed to a significant increase in aromatic hydrocarbon degradation.
authors.affiliation: Division of Microbial Resources, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, CP 6171, CEP 13081-970 Campinas, SP, Brazil. Electronic address: brunamadell@gmail.com.
authors.forename: B M
authors.initials: BM
authors.lastname: Dellagnezze
authors.affiliation: Federal University of São Paulo (UNIFESP), Rua Prof. Artur Riedel, 275, CEP 09972-270, Jd. Eldorado, Diadema, SP, Brazil.
authors.forename: S P
authors.initials: SP
authors.lastname: Vasconcellos
authors.affiliation: Lembiotech (UFC), Federal University of Ceará, Av. Humberto Monte, 2977, Campus do Pici, Bloco 909, 60455-000, Fortaleza, CE, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Angelim
authors.affiliation: Lembiotech (UFC), Federal University of Ceará, Av. Humberto Monte, 2977, Campus do Pici, Bloco 909, 60455-000, Fortaleza, CE, Brazil.
authors.forename: V M M
authors.initials: VMM
authors.lastname: Melo
authors.affiliation: Institute for Coastal Marine Environment (IAMC), Consiglio Nazionale delle Ricerche (CNR) of Messina, Messina, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Santisi
authors.affiliation: Institute for Coastal Marine Environment (IAMC), Consiglio Nazionale delle Ricerche (CNR) of Messina, Messina, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Cappello
authors.affiliation: Division of Microbial Resources, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, CP 6171, CEP 13081-970 Campinas, SP, Brazil.
authors.forename: V M
authors.initials: VM
authors.lastname: Oliveira
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000378:metabolism; D048271:Chitosan; D006838:Hydrocarbons / Q000378:metabolism; D059013:Microbial Consortia; D010578:Petroleum / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D006838:Hydrocarbons; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D048271:Chitosan
keywords: Bioaugmentation; Consortium; Mesocosm; Metagenomic clones; Petroleum