title: Simultaneous anaerobic transformation of tetrachloroethene and carbon tetrachloride in a continuous flow column.
abstract: Tetrachloroethene (PCE) and carbon tetrachloride (CT) were simultaneously transformed in a packed column that was bioaugmented with the Evanite culture (EV). The data presented here have been obtained over a period of 1930days. Initially the column was continuously fed synthetic groundwater with PCE (0.1mM), sulfate (SO4(2-)) (0.2mM) and formate (2.1mM) or lactate (1.1mM), but not CT. In these early stages of the study the effluent H2 concentrations ranged from 7 to 19nM, and PCE was transformed to ethene (ETH) (81 to 85%) and vinyl chloride (VC) (11 to 17%), and SO4(2-) was completely reduced when using either lactate or formate as electron donors. SO4(2-) reduction occurred concurrently with cis-DCE and VC dehalogenation. Formate was a more effective substrate for promoting dehalogenation based on electron donor utilization efficiency. Simultaneous PCE and CT tests found CT (0.015mM) was completely transformed with 20% observed as chloroform (CF) and trace amounts of chloromethane (CM) and dichloromethane (DCM), but no methane (CH4) or carbon disulfide (CS2). PCE transformation to ETH improved with CT addition in response to increases in H2 concentrations to 160nM that resulted from acetate formation being inhibited by either CT or CF. Lactate fermentation was negatively impacted after CT transformation tests, with propionate accumulating, and H2 concentrations being reduced to below 1nM. Under these conditions both SO4(2-) reduction and dehalogenation were negatively impacted, with sulfate reduction not occurring and PCE being transformed to cis-dichloroethene (c-DCE) (52%) and VC (41%). Upon switching to formate, H2 concentrations increased to 40nM, and complete SO4(2-) reduction was achieved, while PCE was transformed to ETH (98%) and VC (1%), with no acetate detected. Throughout the study PCE dehalogenation to ethene was positively correlated with the effluent H2 concentrations.
authors.affiliation: School of Chemical, Biological and Environmental Engineering, 102 Gleeson Hall, Oregon State University, Corvallis, OR 97331, United States. Electronic address: mohammad.azizian@oregonstate.edu.
authors.forename: Mohammad F
authors.initials: MF
authors.lastname: Azizian
authors.affiliation: School of Chemical, Biological and Environmental Engineering, 102 Gleeson Hall, Oregon State University, Corvallis, OR 97331, United States.
authors.forename: Lewis
authors.initials: L
authors.lastname: Semprini
mesh_terms: D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D002251:Carbon Tetrachloride / Q000378:metabolism*; D002725:Chloroform / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D005561:Formates / Q000737:chemistry; D060587:Groundwater / Q000737:chemistry*; D054879:Halogenation; D008697:Methane / Q000378:metabolism; D059013:Microbial Consortia; D010084:Oxidation-Reduction; D013750:Tetrachloroethylene / Q000378:metabolism*; D014752:Vinyl Chloride / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D000085:Acetates; D005030:Ethylenes; D005561:Formates; D014874:Water Pollutants, Chemical; C030544:formic acid; D002725:Chloroform; C036216:ethylene; D002251:Carbon Tetrachloride; D008697:Methane; D013750:Tetrachloroethylene; D014752:Vinyl Chloride
keywords: Bioaugmentation; Carbon tetrachloride; Fermentation; Formate; Hydrogen; Lactate; Tetrachloroethene