title: Proteome analysis for the global proteins in the jejunum tissues of enterotoxigenic Escherichia coli -infected piglets.
abstract: Enterotoxigenic Escherichia coli (ETEC) is a common cause of diarrhea in humans and livestock. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) combined with multidimensional liquid chromatography (LC) and MS analysis was used for screening the differentially expressed proteins in piglet jejunum after ETEC infection. Totally 1,897 proteins were identified with quantitative information in piglet jejunum. We identified 92 differentially expressed proteins in ETEC-induced diarrhea, of which 30 were up regulated and 62 down regulated. Most of the differentially expressed proteins were involved in intestinal function of binding, metabolic process, catalytic activity and immune responses. The inhibition of intestinal immune responses in the jejunum in ETEC-induced diarrhea was also validated by immunobloting and RT-PCR. Our study is the first attempt to analyze the protein profile of ETEC-infected piglets by quantitative proteomics, and our findings could provide valuable information with respect to better understanding the host response to ETEC infection.
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.|University of the Chinese Academy of Sciences, Beijing 10008, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Ren
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.
authors.forename: Jielin
authors.initials: J
authors.lastname: Duan
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.affiliation: Chongqing Key Laboratory of Forage &Herbivorce, College of Animal Science and Technology, Southwest University, Chongqing 400716, China.
authors.forename: Nengzhang
authors.initials: N
authors.lastname: Li
authors.affiliation: Chongqing Key Laboratory of Forage &Herbivorce, College of Animal Science and Technology, Southwest University, Chongqing 400716, China.
authors.forename: Yuanyi
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.|Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients; Hunan Collaborative Innovation Center of Animal Production Safety, Changsha, Hunan, 410128, China.
authors.forename: Bie
authors.initials: B
authors.lastname: Tan
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003967:Diarrhea / Q000276:immunology / Q000382:microbiology; D060467:Disease Resistance; D054307:Enterotoxigenic Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D063990:Gene Ontology; D007583:Jejunum / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D034421:Sus scrofa / Q000276:immunology / Q000382:microbiology*
chemical_list: D020543:Proteome