title: Fatal influenza A (H5N1) virus Infection in zoo-housed Tigers in Yunnan Province, China.
abstract: From 2014 to 2015, three cases of highly pathogenic avian influenza infection occurred in zoo-housed north-east China tigers (Panthera tigris ssp.altaica) and four tigers died of respiratory distress in succession in Yunnan Province, China. We isolated and characterized three highly pathogenic avian influenza A(H5N1) viruses from these tigers. Phylogenetic analysis indicated that A/tiger /Yunnan /tig1404 /2014(H5N1) belongs to the provisional subclade 2.3.4.4e which were novel reassortant influenza A (H5N1) viruses with six internal genes from avian influenza A (H5N2) viruses. The HA gene of the isolated A/tiger /Yunnan /tig1412 /2014(H5N1) virus belongs to the subclade 2.3.2.1b. The isolated A/tiger /Yunnan /tig1508/2015 (H5N1) virus was a novel reassortant influenza A (H5N1) virus with three internal genes (PB2, PB1 and M) from H9N2 virus and belongs to the subclade 2.3.2.1c.
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
authors.forename: Tingsong
authors.initials: T
authors.lastname: Hu
authors.affiliation: Centre for Animal Disease Control and Prevention, Yunnan Province, Kunming 650051, China.
authors.forename: Huanyun
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200030, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Centre for Animal Disease Control and Prevention, Yunnan Province, Kunming 650051, China.
authors.forename: Wendong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Centre for Animal Disease Control and Prevention, Yunnan Province, Kunming 650051, China.|Yunnan Agriculture University, Kunming 650223, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
authors.forename: Zhixiao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Cui
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Deng
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
authors.forename: Quanshui
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Centre for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000836:Animals, Zoo / Q000821:virology; D005260:Female; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D046010:Tigers