title: Enrichment of Dehalococcoides mccartyi spp. from a municipal activated sludge during AQDS-mediated bioelectrochemical dechlorination of 1,2-dichloroethane to ethene.
abstract: The application of bioelectrochemical systems (BES) for the treatment of chloroethanes has been so far limited, in spite of the high frequency that these contaminants are detected at contaminated sites. This work studied the biodegradation of 1,2-dichloroethane (1,2-DCA) in a lab-scale BES, inoculated with a municipal activated sludge and operated under a range of conditions, spanning from oxidative to reductive, both in the presence and in the absence of the humic acid analogue anthraquinone-2,6-disulfonate (AQDS) as a redox mediator. The results showed stable dechlorination of 1,2-DCA to ethene (up to 65±5μmol/Ld), when the BES was operated at a set potential of -300mV vs. SHE, in the presence of AQDS. Sustained filled-and-draw operation resulted in the enrichment of Dehalococcoides mccartyi. The results of this work provide new insights into the applicability of BES for groundwater remediation and the potential interaction between biogeochemistry and 1,2-DCA in humics-rich contaminated aquifers.
authors.affiliation: Water Research Institute (IRSA), National Research Council (CNR), Via Salaria km. 29.300, 00015 Monterotondo (RM), Italy; CERENA, Department of Mining Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; REQUIMTE, Institute of Engineering of Porto, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Leitão
authors.affiliation: Water Research Institute (IRSA), National Research Council (CNR), Via Salaria km. 29.300, 00015 Monterotondo (RM), Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Rossetti
authors.affiliation: CERENA, Department of Mining Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Danko
authors.affiliation: REQUIMTE, Institute of Engineering of Porto, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal.
authors.forename: Henri
authors.initials: H
authors.lastname: Nouws
authors.affiliation: Water Research Institute (IRSA), National Research Council (CNR), Via Salaria km. 29.300, 00015 Monterotondo (RM), Italy. Electronic address: Aulenta@irsa.cnr.it.
authors.forename: Federico
authors.initials: F
authors.lastname: Aulenta
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000378:metabolism*; D005025:Ethylene Dichlorides / Q000737:chemistry / Q000378:metabolism*; D005030:Ethylenes / Q000737:chemistry / Q000378:metabolism*; D054879:Halogenation; D012722:Sewage / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D005025:Ethylene Dichlorides; D005030:Ethylenes; D012722:Sewage; C408124:anthraquinone-2,6-disulfonate; C024565:ethylene dichloride; C036216:ethylene
keywords: 1,2-Dichloroethane; Activated sludge; Anthraquinone-2,6-disulfonate (AQDS); Bioelectrochemical systems; Dehalococcoides mccartyi; Redox mediator