title: Evaluation of 660 nm LED light irradiation on the strategies for treating experimental periodontal intrabony defects.
abstract: This study aims to investigate the therapeutic value of 660 nm light-emitting diode (LED) light irradiation on the strategies for treating experimental periodontal intrabony defects in vivo. Large-sized periodontal intrabony defects were created bilaterally on the mesial aspect of the maxillary second molars of 48 Sprague-Dawley rats, and the rats were equally divided into four treatment groups with primary wound intention (n = 6/treatment/time point), including open flap debridement alone (OD), barrier membrane alone (MB), xenograft alone (BG), and xenograft plus barrier membrane (MG). Each group received daily 0 or 10 J/cm(2) LED light irradiation. The animals were sacrificed after 1 or 4 weeks. The treatment outcome was evaluated by gross observation of wound dehiscence and healing, micro-CT imaging for osteogenesis, and histological assessments for inflammatory cell infiltration and periodontal reattachment. With LED light irradiation, the extent of wound dehiscence was reduced, wound closure was accelerated, epithelial downgrowth was prevented, inflammation was reduced, and periodontal reattachment was promoted in all treatment strategies. Significant reduction of inflammation with LED light irradiation was noted at 1 week in the groups BG and MG (p < 0.05). Osteogenesis was significantly promoted only in the group OD at both time points (p < 0.05). Our study showed that 660 nm LED light accelerates mucoperiosteal flap healing and periodontal reattachment. However, the enhancement of osteogenesis appeared to be limited while simultaneously treating with a barrier membrane or xenograft.
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Chih-Yun
authors.initials: CY
authors.lastname: Tao
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei, 100, Taiwan.
authors.forename: Ning
authors.initials: N
authors.lastname: Lee
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.|Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei, 100, Taiwan.
authors.forename: Hao-Chieh
authors.initials: HC
authors.lastname: Chang
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei, 100, Taiwan.
authors.forename: Connie
authors.initials: C
authors.lastname: Yang
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei, 100, Taiwan.
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Yu
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan. changpc@ntu.edu.tw.|Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei, 100, Taiwan. changpc@ntu.edu.tw.
authors.forename: Po-Chun
authors.initials: PC
authors.lastname: Chang
mesh_terms: D000818:Animals; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D010012:Osteogenesis / Q000528:radiation effects; D010510:Periodontal Diseases / Q000532:radiotherapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000528:radiation effects
keywords: Guided tissue regeneration; Low level laser therapy; Osteogenesis; Wound healing