title: Effects of Size-Fractionated Particulate Matter on Cellular Oxidant Radical Generation in Human Bronchial Epithelial BEAS-2B Cells.
abstract: The aim of the present study was to investigate the effects of size-fractionated (i.e., <1; 1-2.5, and 2.5-10 µm in an aerodynamic diameter) ambient particulate matter (PM) on reactive oxygen species (ROS) activity and cell viability in human bronchial epithelial cells (BEAS-2B). The PM samples were collected from an urban site (uPM) in Beijing and a steel factory site (sPM) in Anshan, China, from March 2013 to December 2014. Metal elements, organic and elemental carbon, and water-soluble inorganic ions in the uPM and sPM were analyzed. The cell viability and ROS generation in PM-exposed BEAS-2B cells were measured by MTS and DCFH-DA. The results showed that both uPM and sPM caused a decrease in the cell viability and an increase in ROS generation. The level of ROS measured in sPM1.0 was approximately triple that in uPM1.0. The results of correlation analysis showed that the ROS activity and cytotoxicity were related to different PM composition. Moreover, deferoxamine (DFO) significantly prevented the increase of ROS generation and the decrease of cell viability. Taken together, our results suggest that the metals absorbed on PM induced oxidant radical generation in BEAS-2B cells that could lead to impairment of pulmonary function.
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. guanlongfei12@mails.ucas.ac.cn.
authors.forename: Longfei
authors.initials: L
authors.lastname: Guan
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ruiwei08@mails.ucas.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Rui
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. bairu10@mails.ucas.ac.cn.
authors.forename: Ru
authors.initials: R
authors.lastname: Bai
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. zhangw@ucas.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. zhangfang@ucas.ac.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. dingwj@ucas.ac.cn.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Ding
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D000068476:Beijing; D001980:Bronchi / Q000166:cytology; D002244:Carbon / Q000032:analysis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002681:China; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008670:Metals / Q000032:analysis / Q000633:toxicity*; D010316:Particle Size; D052638:Particulate Matter / Q000032:analysis / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013232:Steel
chemical_list: D000393:Air Pollutants; D008670:Metals; D052638:Particulate Matter; D017382:Reactive Oxygen Species; D013232:Steel; D002244:Carbon
keywords: chemical composition; correlation analysis; cytotoxicity; particulate matter; size-fraction