title: Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis.
abstract: The development of precision medicine, together with the multiplication of targeted therapies and associated molecular biomarkers, call for major progress in genetic analysis methods, allowing increased multiplexing and the implementation of more complex decision trees, without cost increase or loss of robustness. We present a platform combining droplet microfluidics and magnetic tweezers, performing RNA purification, reverse transcription and amplification in a fully automated and programmable way, in droplets of 250nL directly sampled from a microtiter-plate. This platform decreases sample consumption about 100 fold as compared to current robotized platforms and it reduces human manipulations and contamination risk. The platform's performance was first evaluated on cell lines, showing robust operation on RNA quantities corresponding to less than one cell, and then clinically validated with a cohort of 21 breast cancer samples, for the determination of their HER2 expression status, in a blind comparison with an established routine clinical analysis.
authors.affiliation: Institut Curie, PSL Research University, Laboratoire Physicochimie, CNRS/UMR 168, Institut Pierre-Gilles de Gennes, MMBM group, Paris, France.
authors.forename: Davide
authors.initials: D
authors.lastname: Ferraro
authors.affiliation: APHP Hôpital Saint-Louis, Molecular Oncology Unit, University Paris-Diderot, INSERM/CNRS, UMR944/7212, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Champ
authors.affiliation: Institut Curie, PSL Research University, Laboratoire Physicochimie, CNRS/UMR 168, Institut Pierre-Gilles de Gennes, MMBM group, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Teste
authors.affiliation: Institut Curie, PSL Research University, Laboratoire Physicochimie, CNRS/UMR 168, Institut Pierre-Gilles de Gennes, MMBM group, Paris, France.
authors.forename: Marco
authors.initials: M
authors.lastname: Serra
authors.affiliation: LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Malaquin
authors.affiliation: Institut Curie, PSL Research University, Laboratoire Physicochimie, CNRS/UMR 168, Institut Pierre-Gilles de Gennes, MMBM group, Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
authors.affiliation: APHP Hôpital Saint-Louis, Molecular Oncology Unit, University Paris-Diderot, INSERM/CNRS, UMR944/7212, Paris, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: de Cremoux
authors.affiliation: Institut Curie, PSL Research University, Laboratoire Physicochimie, CNRS/UMR 168, Institut Pierre-Gilles de Gennes, MMBM group, Paris, France.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Descroix
mesh_terms: D002138:Calibration; D045744:Cell Line, Tumor; D006801:Humans; D060328:Magnetic Phenomena; D044085:Microfluidics / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases