title: Regional Differences of Proteins Expressing in Adipose Depots Isolated from Cows, Steers and Bulls as Identified by a Proteomic Approach.
abstract: Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis. Two different adipose depots (i.e. intramuscular and omental) were collected from cows (n = 7), steers (n = 7), or bulls (n = 7). LC-MS/MS revealed a total of 55 and 35 proteins in IMAT and OMAT, respectively. Of the 55 proteins identified, 44, 40, and 42 proteins were confirmed to be differentially expressed in IMAT of cows, steers, and bulls, respectively. In OMAT of cows, steers, and bulls, 33, 33, and 22 were confirmed to be differentially expressed, respectively. Tropomyosin (TPM) 1, TPM 2, and TPM3 were subjected to verification by quantitative PCR and western blot analysis in IMAT and OMAT of Hanwoo cows, steers, and bulls as key factors closely associated with muscle development. Both mRNA levels and protein levels of TPM1, TPM2, and TPM3 in IMAT were lower in bulls compared to in cows or steers suggesting that they were positively correlated with marbling score and quality grade. Our results may aid the regulation of marbling development and improvement of meat quality grades in beef cattle.
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Jin Hyoung
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Animal Products Utilization Division, National Institute of Animal science, Jeonju 565-851, Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Ra Ham
authors.initials: RH
authors.lastname: Lee
authors.affiliation: Animal Products Utilization Division, National Institute of Animal science, Jeonju 565-851, Korea.
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Park
authors.affiliation: Aging Research Institute, Korea Research Institute of Bioscience & BioTechnology, Daejeon 34141, Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Pohang Center for Evaluation of Biomaterials, Pohang Technopark, Pohang 37668, Korea.
authors.forename: Seon-Min
authors.initials: SM
authors.lastname: Park
authors.affiliation: Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Mokpo 534-729, Korea.
authors.forename: Jae-Cheon
authors.initials: JC
authors.lastname: Shin
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Jeon
authors.affiliation: Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Mokpo 534-729, Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Shim
authors.affiliation: Department of Animal Science, College of Agricultural and Life Science, Chonbuk National University, Jeonju 54896, Korea.
authors.forename: Nag-Jin
authors.initials: NJ
authors.lastname: Choi
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea.
authors.forename: Kang Seok
authors.initials: KS
authors.lastname: Seo
authors.affiliation: Department of Pharmacy, Keimyung University, Daegu 704-701, Korea.
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Cho
authors.affiliation: Department of Biomedical Engineering, Konyang University, Daejeon 35365, Korea.
authors.forename: MinSeok S
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Applied Bioscience, CHA University, Seongnam 463-836, Korea.
authors.forename: Sungho
authors.initials: S
authors.lastname: Ko
authors.affiliation: Department of Prosthodontics, School of Dentistry and Institute of Oral Bio-Science and Research Institute of Clinical Medicine, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Seo
authors.affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Seung-Youp
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University, Jeonju 651-756, Korea.
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Chae
authors.affiliation: Animal Nutritional Physiology Team, National Institute of Animal Science, Jeonju 565-851, Korea.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Lee
keywords: Adipose Depots; Hanwoo; Liquid Chromatography-tandem Mass Spectrometry; Marbling; Meat Quality