title: TMEPAI increases lysosome stability and promotes autophagy.
abstract: Autophagy is emerging as a critical response of normal and cancer cells to environmental changes and plays an important role in cell metabolism and maintenance of damaged organelles. Transmembrane prostate androgen-induced protein (TMEPAI) is a pro-tumorigenic factor with high expression in tumor cells. In this study, we showed that depletion of TMEPAI leads to lysosomal labilization and inhibits autophagy. Further study showed that the inhibition of autophagy induced by the depletion of TMEPAI is involved in regulation of Beclin-1. Depletion of TMEPAI increases the sensitivity of cancer cells to chemotherapeutic drugs. Our study reveals the role of TMEPAI in promoting lysosome stability and autophagy, which might be used as a target for cancer chemotherapeutic treatment.
authors.affiliation: School of Biotechnology, Tianjin University of Science and Technology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, China.
authors.forename: Shenheng
authors.initials: S
authors.lastname: Luo
authors.affiliation: School of Biotechnology, Tianjin University of Science and Technology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.affiliation: School of Biotechnology, Tianjin University of Science and Technology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lv
authors.affiliation: School of Biotechnology, Tianjin University of Science and Technology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jing
authors.affiliation: School of Biotechnology, Tianjin University of Science and Technology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, China.
authors.forename: Yuyin
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Biotechnology, Tianjin University of Science and Technology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Biotechnology, Tianjin University of Science and Technology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, China. Electronic address: diaoaipo@tust.edu.cn.
authors.forename: Aipo
authors.initials: A
authors.lastname: Diao
mesh_terms: D000072283:A549 Cells; D001343:Autophagy; D000071186:Beclin-1 / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008247:Lysosomes / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D061986:MCF-7 Cells; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D000071186:Beclin-1; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C412868:PMEPA1 protein, human
keywords: Autophagy; Beclin-1; Cancer cell; Lysosome; TMEPAI