title: Optimization of microwave-assisted extraction of polysaccharide from Psidium guajava L. fruits.
abstract: This study deals with the optimization of microwave assisted extraction of polysaccharide from Psidium guajava L. fruit using Response surface methodology. To evaluate the effect of three independent variables, Water to plant material ratio, microwave power used for extraction and Irradiation time, central composite design has been employed. The yield is considered as dependent variable. The design model estimated the optimum yield of 6.81677% at 200W microwave power level, 3:1 water to plant material ratio and 20min of irradiation time. Three factors three levels Central composite design coupled with RSM was used to model the extraction process. ANOVA was performed to find the significance of the model. The polysaccharide extracted using microwave assisted extraction process was analyzed using FTIR Spectroscopy.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395, Sarojini Naidu Road, Coimbatore, 641044, India. Electronic address: amutha.jr@gmail.com.
authors.forename: Michael Antony Samy
authors.initials: MA
authors.lastname: Amutha Gnana Arasi
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395, Sarojini Naidu Road, Coimbatore, 641044, India.
authors.forename: Manchineela
authors.initials: M
authors.lastname: Gopal Rao
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395, Sarojini Naidu Road, Coimbatore, 641044, India.
authors.forename: Janardanan
authors.initials: J
authors.lastname: Bagyalakshmi
mesh_terms: D005638:Fruit / Q000737:chemistry*; D008872:Microwaves; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D031647:Psidium / Q000737:chemistry*
chemical_list: D011134:Polysaccharides
keywords: Central composite design; Extraction; Microwave; Optimization; Response surface methodology