title: Association of EGF Receptor and NLRs signaling with Cardiac Inflammation and Fibrosis in Mice Exposed to Fine Particulate Matter.
abstract: ЄAmbient fine particulate matter (PM2.5 ) could induce cardiovascular diseases (CVD), but the mechanism remains unknown. To investigate the roles of epidermal growth factor receptor (EGFR) and NOD-like receptors (NLRs) in PM2.5 -induced cardiac injury, we set up a BALB/c mice model of PM2.5 -induced cardiac inflammation and fibrosis with intratracheal instillation of PM2.5 suspension (4.0 mg/kg b.w.) for 5 consecutive days (once per day). After exposure, we found that mRNA levels of CXCL1, interleukin (IL)-6, and IL-18 were elevated, but interestingly, mRNA level of NLRP12 was significant decreased in heart tissue from PM2.5 -induced mice compared with those of saline-treated mice using real-time PCR. Protein levels of phospho-EGFR (Tyr1068), phospho-Akt (Thr308), NLRP3, NF-κB-p52/p100, and NF-κB-p65 in heart tissue of PM2.5 -exposed mice were all significantly increased using immunohistochemistry or Western blotting. Therefore, PM2.5 exposure could induce cardiac inflammatory injury in mice, which may be involved with EGFR/Akt signaling, NLRP3, and NLRP12.
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou, 450001, Henan, People's Republic of China.
authors.forename: Yuefei
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Geriatrics, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, 450014, Henan, People's Republic of China.
authors.forename: Zhaoke
authors.initials: Z
authors.lastname: Wu
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou, 450001, Henan, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou, 450001, Henan, People's Republic of China.
authors.forename: Shuyin
authors.initials: S
authors.lastname: Duan
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou, 450001, Henan, People's Republic of China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Public Health, Xinxiang Medical University, Xinxiang, 453003, Henan, People's Republic of China. wdwu2013@126.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Public Health, Zhengzhou University, Zhengzhou, 450001, Henan, People's Republic of China. heartnessff@126.com.
authors.forename: Feifei
authors.initials: F
authors.lastname: Feng
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D054360:Chemokine CXCL1 / Q000235:genetics / Q000378:metabolism; D004719:Endomyocardial Fibrosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D052003:NF-kappa B p52 Subunit / Q000235:genetics / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics / Q000378:metabolism*; D010316:Particle Size; D052638:Particulate Matter / Q000009:adverse effects*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism
chemical_list: D054360:Chemokine CXCL1; C475895:Cxcl1 protein, mouse; D020382:Interleukin-18; D015850:Interleukin-6; D047908:Intracellular Signaling Peptides and Proteins; D052003:NF-kappa B p52 Subunit; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C553199:NLRP12 protein, mouse; C492716:Nlrp3 protein, mouse; D052638:Particulate Matter; D012333:RNA, Messenger; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; C577686:interleukin-6, mouse; C541485:EGFR protein, mouse; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt
keywords: Cardiac Inflammation and Fibrosis; Epidermal Growth Factor Receptor; NLRP12; NLRP3; PM2.5