title: DNA-based identification of forensically important species of Sarcophagidae (Insecta: Diptera) from Rio de Janeiro, Brazil.
abstract: Sarcophagidae, or flesh flies, are of great importance in forensic entomology, but their effective application requires precise taxonomic identification, which relies almost exclusively on characteristics of the male genitalia. Given that female flies and larvae are most abundant in animal carcasses or on corpses, precise morphological identification can be difficult; therefore, DNA sequencing can be an additional tool for use in taxonomic identification. This paper analyzes part of the mitochondrial cytochrome c oxidase subunit I (COI) gene from three Sarcophagidae species of forensic importance in the City of Rio de Janeiro: Oxysarcodexia fluminensis, Peckia chrysostoma, and Peckia intermutans. COI fragments of 400 bp from 36 specimens of these three species were sequenced. No intraspecific differences were found among specimens of O. fluminensis, but P. chrysostoma and P. intermutans each had two haplotypes, ranging from 0 to 0.7%. The interspecific divergence was 8.5-11.6%, corroborating previously reported findings.
authors.affiliation: DIMAV, Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, RJ, Brasil.|Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: K S
authors.initials: KS
authors.lastname: Napoleão
authors.affiliation: Departamento de Entomologia, Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: C A
authors.initials: CA
authors.lastname: Mello-Patiu
authors.affiliation: Laboratório de Entomologia Forense, Instituto de Criminalística Carlos Éboli, Departamento Geral de Polícia Técnico Científica, Polícia Civil do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Oliveira-Costa
authors.affiliation: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: D M
authors.initials: DM
authors.lastname: Takiya
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Silva
authors.affiliation: DIMAV, Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, RJ, Brasil.|Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors.forename: R S
authors.initials: RS
authors.lastname: Moura-Neto
mesh_terms: D000818:Animals; D001938:Brazil; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D010802:Phylogeny; D058493:Sarcophagidae / Q000145:classification / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D003576:Electron Transport Complex IV