title: The essential oils component p-cymene induces proton leak through Fo-ATP synthase and uncoupling of mitochondrial respiration.
abstract: Essential oils can be used as antimicrobial, antioxidant, and anticarcinogenic agents or to preserve and give flavors to foods. The activity of phenolic-rich essential oils has been observed in fractions containing thymol and carvacrol which show synergistic effects with their precursor p-cymene. Their mode of action is related to several targets in the cell but specific mechanisms of activity and cytotoxic effects remain poorly characterized. Given the importance of mitochondria for cellular functions and their critical role in a vast number of diseases, this work evaluated the effects of p-cymene on mitochondrial functions. It was observed that p-cymene did not change the oxygen consumption by respiratory chain (state 2 respiration). However, p-cymene decreased the mitochondrial membrane potential (Δψ), depressed the rate of ADP phosphorylation (state 3), and stimulated the oxygen consumption after phosphorylation of ADP (state 4). The respiratory control ratio (state 3/state 4) was decreased as a consequence of the inhibition of state 3 and stimulation of state 4 respiration but the ADP/O index remained unaltered as well as the mitochondrial Ca(2+) fluxes. Moreover, p-cymene did not induce mitochondrial membrane disruption but depressed the Δψ, and the stimulatory effect observed on state 4, similar to the effect observed on state 2 respiration plus ATP, was inhibited by oligomycin. These effects suggest that p-cymene allows a proton leak through the Fo fraction of the phosphorylative system, changing the mitochondrial proton motive force and ATP synthesis capacity. Therefore, these data suggest mitochondria as a target for p-cymene toxicity action mechanisms.
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, Portugal; Laboratory of Biochemistry, Faculty of Pharmacy, University of Coimbra, Portugal.
authors.forename: José Ba
authors.initials: JB
authors.lastname: Custódio
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, Portugal; Laboratory of Biochemistry, Faculty of Pharmacy, University of Coimbra, Portugal.
authors.forename: Mariana V
authors.initials: MV
authors.lastname: Ribeiro
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, Portugal; Laboratory of Biochemistry, Faculty of Pharmacy, University of Coimbra, Portugal.
authors.forename: Filomena Sg
authors.initials: FS
authors.lastname: Silva
authors.affiliation: Center of Pharmaceutical Studies, University of Coimbra, Portugal; Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, Portugal.
authors.forename: Marisa
authors.initials: M
authors.lastname: Machado
authors.affiliation: Center of Pharmaceutical Studies, University of Coimbra, Portugal; Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, Portugal.
authors.forename: M Céu
authors.initials: MC
authors.lastname: Sousa
keywords: ATP synthase; antimicrobial; essential oils; food additive; mitochondrial respiration; p-cymene; proton leak; uncoupling