title: The catalytic activity of Ag2S-montmorillonites as peroxidase mimetic toward colorimetric detection of H2O2.
abstract: Nanocomposites based on silver sulfide (Ag2S) and Ca-montmorillonite (Ca(2+)-MMT) were synthesized by a simple hydrothermal method. The nanocomposites were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectra (FTIR). The as-prepared Ag2S-MMT nanocomposites were firstly demonstrated to possess intrinsic peroxidase-like activity and could rapidly catalytically oxidize the substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue product which can be seen by the naked eye in only one minute. The experimental results revealed that the Ag2S-MMT nanocomposites exhibit higher thermal durance. Based on the TMB-H2O2 catalyzed color reaction, the Ag2S-MMT nanocomposites were exploited as a new type of biosensor for detection and estimation of H2O2 through a simple, cheap and selective colorimetric method.
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China. Electronic address: qyliu@sdust.edu.cn.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Leyou
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Xinpei
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Xintian
authors.initials: X
authors.lastname: Lv
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Yanyuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Academy of Science & Technology, China University of Petroleum, Dongying 257061, PR China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Yin
mesh_terms: D000818:Animals; D001546:Bentonite / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques; D002384:Catalysis; D003124:Colorimetry; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D008892:Milk / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D018030:Silver Compounds / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D018030:Silver Compounds; D001546:Bentonite; C013251:silver sulfide; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: Ag(2)S; Ca-montmorillonite; Colorimetric; H(2)O(2); Peroxidase