title: Targeting of BUB1b Gene Expression in Sentinel Lymph Node Biopsies of Invasive Breast Cancer in Iranian Female Patients.
abstract: Detection of micrometastasis in sentinel lymph nodes (SLNs) is a very useful tool for appropriate assessment of the clinical stage of disease in breast cancer patients. Early identification of clinically relevant disease could lead to early treatment or staging approaches for breast cancer patient. Micrometastases in SLNs of women with invasive breast cancer are of great significance in this context. In this study we examined SLN biopsies considered to have small numbers of cancerous cells by real time RT-PCR. All of the samples underwent immunohistochemical staining for cytokeratin for confirmation of the presence or absence of micrometastases. BUB1b expression assay of selected patients with and without metastasis showed overexpression in the former, but not in normal breast and lymph node tissue. Our results may be taken into account in the discussion about the merits of routine use of molecular assessment in pathogenetic studies of SLNs.
authors.affiliation: Department of Medical Genetics, School of Medicine,Shahid Beheshti University of Medical Sciences, Tehran, Iran E-mail: movafagh.a@sbmu.ac.ir.
authors.forename: Neda
authors.initials: N
authors.lastname: Mansouri
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
authors.forename: Arezou
authors.initials: A
authors.lastname: Sayad
authors.forename: Atefeh
authors.initials: A
authors.lastname: Heidary Pour
authors.forename: Mohammad
authors.initials: M
authors.lastname: Taheri
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Soleimani
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Mirzaei
authors.forename: Shohreh
authors.initials: S
authors.lastname: Alizadeh Shargh
authors.forename: Eznollah
authors.initials: E
authors.lastname: Azargashb
authors.forename: Haleh
authors.initials: H
authors.lastname: Bazmi
authors.forename: Hossein
authors.initials: H
authors.lastname: Allah Moradi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Zandnia
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Nilofar
authors.initials: N
authors.lastname: Massoudi
authors.forename: S A
authors.initials: SA
authors.lastname: Mortazavi-Tabatabaei
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D018797:Cell Cycle Proteins / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D061206:Neoplasm Micrometastasis; D009367:Neoplasm Staging; D011379:Prognosis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071036:Sentinel Lymph Node / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D021701:Sentinel Lymph Node Biopsy
chemical_list: C584279:BUB1B protein, human; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases