title: DNA barcoding reveals species composition of sharks and rays in the Indian commercial fishery.
abstract: DNA barcoding was successfully used for the accurate identification of chondrichthyans in the Indian commercial marine fishery. About 528 specimens of 111 chondrichthyan species and 34 families, collected from the Indian EEZ, were barcoded for a 655 bp region of the mitochondrial gene cytochrome c oxidase subunit 1 (COI). Generally, five specimens per species were barcoded, but numbers ranged from 2 to 13. The average Kimura 2 parameter (K2P) distance separating individuals within species was 0.32%, and the average distance separating species within genera was 6.73%. Ten species were suggested as putative new species requiring formal descriptions. Based on the morphology and molecular support, 11 elasmobranch species were confirmed first records for Indian waters. The present study confirms the ability of DNA barcoding for the accurate identification of sharks, rays, and their products from Indian waters.
authors.affiliation: a National Bureau of Fish Genetic Resources, Central Marine Fisheries Research Institute Campus Ernakulam North , Kochi , Kerala , India.
authors.forename: K K
authors.initials: KK
authors.lastname: Bineesh
authors.affiliation: b Central Marine Fisheries Research Institute , Ernakulam North , Kochi , Kerala , India.
authors.forename: A
authors.initials: A
authors.lastname: Gopalakrishnan
authors.affiliation: b Central Marine Fisheries Research Institute , Ernakulam North , Kochi , Kerala , India.
authors.forename: K V
authors.initials: KV
authors.lastname: Akhilesh
authors.affiliation: a National Bureau of Fish Genetic Resources, Central Marine Fisheries Research Institute Campus Ernakulam North , Kochi , Kerala , India.
authors.forename: K A
authors.initials: KA
authors.lastname: Sajeela
authors.affiliation: b Central Marine Fisheries Research Institute , Ernakulam North , Kochi , Kerala , India.
authors.forename: E M
authors.initials: EM
authors.lastname: Abdussamad
authors.affiliation: b Central Marine Fisheries Research Institute , Ernakulam North , Kochi , Kerala , India.
authors.forename: N G K
authors.initials: NGK
authors.lastname: Pillai
authors.affiliation: a National Bureau of Fish Genetic Resources, Central Marine Fisheries Research Institute Campus Ernakulam North , Kochi , Kerala , India.
authors.forename: V S
authors.initials: VS
authors.lastname: Basheer
authors.affiliation: c National Bureau of Fish Genetic Resources , Dilkusha , Lucknow , India.
authors.forename: J K
authors.initials: JK
authors.lastname: Jena
authors.affiliation: d CSIRO National Research Collections Australia , Hobart , Tasmania , Australia.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Ward
mesh_terms: D000818:Animals; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics; D005398:Fisheries; D007194:India; D010802:Phylogeny; D012754:Sharks / Q000145:classification* / Q000235:genetics; D012860:Skates, Fish / Q000145:classification* / Q000235:genetics; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D029941:Fish Proteins; D003576:Electron Transport Complex IV
keywords: Barcoding; COI; Indian Ocean; chondrichthyans; identification; taxonomy