title: Huang Qi Huai Granules Induce Apoptosis in Acute Lymphoblastic Leukemia Cells through the Akt/FoxO1 Pathway.
abstract: OBJECTIVE
  In recent years, a traditional Chinese medicine named Huang Qi Huai (HQH) has been frequently used in China for solid tumor therapy. However, the role of HQH on leukemia cells and its underlying mechanisms have not been elucidated. In this study, we investigated the effect of HQH on the proliferation and apoptosis of acute lymphoblastic leukemia (ALL) cell lines.
  
  
  METHODS
  Sup-B15 and Nalm-6 cells were treated with gradient doses of HQH for 24, 48 or 72 h. Cell viability was measured using a CCK8 assay and cell cycle distribution and apoptosis levels were analyzed using flow cytometry. Western blotting was used to assess the levels of proteins associated with the apoptotic pathway.
  
  
  RESULTS
  The results revealed that cell survival decreased significantly with increasing concentrations of HQH. HQH induced G2 cell-cycle arrest and cell apoptosis in a dose-dependent manner. HQH inhibited phosphorylated-Akt, phosphorylated- FoxO1 and Bcl2 expression and upregulated Bim, cleaved-caspase-3 and Bax expression in a dose-dependent manner, which suggests that HQH induces the apoptosis of ALL cells via the Akt/FoxO1 pathway.
  
  
  CONCLUSIONS
  HQH is a potential complementary agent for the treatment of acute lymphoblastic leukemia.
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.forename: Ming
authors.initials: M
authors.lastname: Lin
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Zhou
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Runming
authors.initials: R
authors.lastname: Jin
authors.forename: Fen
authors.initials: F
authors.lastname: Zhou
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D027885:Astragalus propinquus; D000072224:Bcl-2-Like Protein 11 / Q000378:metabolism; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D000071161:Forkhead Box Protein O1 / Q000378:metabolism*; D006801:Humans; D008516:Medicine, Chinese Traditional; D010766:Phosphorylation / Q000187:drug effects; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D000072224:Bcl-2-Like Protein 11; D004365:Drugs, Chinese Herbal; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C027492:Huang Qi; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3