title: Noninvasive Imaging of Natural Killer Cell-Mediated Apoptosis in a Mouse Tumor Model.
abstract: Natural killer (NK) cells are cytotoxic lymphocytes that induce apoptosis in cancer cells infected with viruses and bacteria through a caspase-3-dependent pathway. Effective NK cell-based immunotherapy requires highly sensitive imaging tools for in vivo monitoring of the dynamic events involved in apoptosis. Here, we describe a noninvasive bioluminescence imaging approach to determine the antitumor effects of NK cell-based therapy by serial imaging of caspase-3-dependent apoptosis in a mouse model of human glioma.
authors.affiliation: Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South Korea.
authors.forename: Thoudam Debraj
authors.initials: TD
authors.lastname: Singh
authors.affiliation: Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South Korea. jaetae@knu.ac.kr.|Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, South Korea. jaetae@knu.ac.kr.
authors.forename: Jaetae
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu, South Korea. jeon9014@gmail.com.|Leading-edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu, South Korea. jeon9014@gmail.com.
authors.forename: Yong Hyun
authors.initials: YH
authors.lastname: Jeon
mesh_terms: D000818:Animals; D017209:Apoptosis; D015374:Biosensing Techniques / Q000379:methods*; D053148:Caspase 3 / Q000378:metabolism*; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D005910:Glioma / Q000378:metabolism / Q000628:therapy*; D006801:Humans; D016219:Immunotherapy, Adoptive; D007694:Killer Cells, Natural / Q000637:transplantation*; D008163:Luminescent Measurements; D051379:Mice; D009368:Neoplasm Transplantation
chemical_list: C505407:CASP3 protein, human; D053148:Caspase 3
keywords: Apoptosis; Caspase-3 biosensor; Immunotherapy; Natural killer cells; Renilla luciferase; Reporter gene