title: Transcriptomic profile of tobacco in response to Alternaria longipes and Alternaria alternata infections.
abstract: Tobacco brown spot caused by Alternaria fungal species is one of the most damaging diseases, and results in significant yield losses. However, little is known about the systematic response of tobacco to this fungal infection. To fill this knowledge gap, de novo assemblies of tobacco leaf transcriptomes were obtained in cultivars V2 and NC89 after the inoculation of either Alternaria longipes (AL) or Alternaria alternata (AA) at three different time points. We studied the gene expression profile of each cultivar-pathogen combination, and identified eight differentially expressed genes shared among all combinations. Gene ontology enrichment analysis of the differentially expressed genes revealed key components during the fungal infection, which included regulation of gene expression (GO:0010468), regulation of RNA metabolic process (GO:0051252), tetrapyrrole binding (GO:0046906), and external encapsulating structure (GO:0030312). Further analyses of the continuously upregulated/downregulated genes and the resistance genes demonstrated that the gene expression profile upon fungal infection was contingent on the specific cultivar and pathogen. In conclusion, this study provides a solid foundation for the investigation of plant-pathogen interaction, and is of great importance for disease prevention and molecular breeding.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Shengchang
authors.initials: S
authors.lastname: Duan
authors.affiliation: Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Yunnan Agricultural University, Kunming, 650201, China.|Yunnan Research Institute for Local Plateau Agriculture and Industry, Kunming, 650201, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Wan
authors.affiliation: Public Technical Service Center, Kunming Institute of Zoology, Chinese Academy of Science, Kunming, 650223, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: He
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Ma
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Long
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Yangzi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000528:Alternaria / Q000502:physiology*; D015536:Down-Regulation / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D005810:Multigene Family; D009336:Necrosis; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D014026:Nicotiana / Q000235:genetics* / Q000382:microbiology*; D059467:Transcriptome; D015854:Up-Regulation / Q000235:genetics