title: Ketoprofen mesoporous silica nanoparticles SBA-15 hard gelatin capsules: preparation and in vitro/in vivo characterization.
abstract: SBA-15 is used to enhance the bioavailability of poorly soluble ketoprofen (KP) through stabilization of its amorphous state. Additionally, the current work provides a complete in vitro and in vivo study on preformulated KP-SBA-15 sample and formulated KP-SBA-15 in hard gelatin capsule. Loading of KP was done by a novel method called immersion-rotavapor method. KP was quantified by extraction and thermal gravimetric analysis (TGA). Characterization of the loaded SBA-15 sample was done by high resolution transmission electron microscopy (HRTEM), small angle X-ray diffraction (SAXRD), nitrogen adsorption/desorption isotherms, differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy and dissolution profiles. The loaded sample was formulated in hard gelatin capsule. The anti-inflammatory and analgesic studies were carried out on 24 adult male albino rats. TGA and extraction results showed 54.4 wt% of drug incorporated. Characterization of KP-SBA-15 sample confirmed the successful encapsulation of KP into the carrier pores in a molecular amorphous state. Additionally, loading of KP did not affect the mesoporous internal structure. During the first 5 min, the dissolution study showed very high release rates; nearly 50% of KP was released. These results were reflected on the in vivo study resulting in 82% inhibition in edema after 1 h and maximum analgesia after 30 min from the administration of the formulated sample. SBA-15 mesoporous silica nanoparticle proved to be a very promising drug delivery carrier that can be used as a facile way to enhance the bioavailability of poorly soluble drugs.
authors.affiliation: a Department of Pharmaceutics and Industrial Pharmacy , College of Pharmacy, Cairo University , Cairo , Egypt and.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Abd-Elrahman
authors.affiliation: a Department of Pharmaceutics and Industrial Pharmacy , College of Pharmacy, Cairo University , Cairo , Egypt and.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: El Nabarawi
authors.affiliation: b Department of Pharmaceutics and Industrial Pharmacy , College of Pharmacy, Misr University for Science and Technology , Sixth of October City, Giza , Egypt.
authors.forename: Doaa H
authors.initials: DH
authors.lastname: Hassan
authors.affiliation: b Department of Pharmaceutics and Industrial Pharmacy , College of Pharmacy, Misr University for Science and Technology , Sixth of October City, Giza , Egypt.
authors.forename: Amal A
authors.initials: AA
authors.lastname: Taha
mesh_terms: D000327:Adsorption; D000818:Animals; D001682:Biological Availability; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002214:Capsules / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005780:Gelatin / Q000737:chemistry*; D007660:Ketoprofen / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D002214:Capsules; D004337:Drug Carriers; C509969:SBA-15; D014867:Water; D012822:Silicon Dioxide; D005780:Gelatin; D007660:Ketoprofen
keywords: In vitro in vivo study; SBA-15; immersion-rotavapor loading method; loading method mesoporous silica nanoparticle