title: Inhibitory effect of endostatin gene therapy combined with phosphorus-32 colloid on tumour growth in Wistar rats.
abstract: Eighty healthy male Wistar rats, aged 5 weeks, weighing 100-120 g, were utilized for establishing tumour-bearing models by immediate Walker-256 cancerous ascites injection and randomly divided to four groups (n=20) treated with 0.2 ml solution containing saline, (32)P-colloid (0.3 mCi), endostatin gene (20 μg), endostatin gene combined with colloid (32)P. The effect of endostatin combined with a small dose of (32)P-colloidal on tumour growth in vivo was evaluated and the potential mechanism underlying the combined therapy was explored. We found that (32)P-colloid combined with endostatin exhibited higher inhibitory effect upon tumour growth compared with application of (32)P-colloid or endostatin alone, although three therapies all significantly inhibited tumour growth compared with saline control group. The higher inhibitory effect of (32)P-colloid combined with endostatin upon tumour growth might be attributed to a synergistic effect of inhibiting angiogenesis by endostatin and inducing apoptosis by (32)P-colloid, as demonstrated by microvessel density (MVD) and apoptotic index (AI) measurement. Combined therapy of (32)P-colloid and endostatin probably serves as a novel and efficacious therapy of tumour growth.
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Huiqi
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Gerontology, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of PET-CT, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Fu
authors.affiliation: Department of Radiology, The Fourth Hospital of Harbin Medical University, China Molecular Imaging Research Center of Harbin Medical University, China baozhong_shen@sina.com.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Shen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003102:Colloids / Q000008:administration & dosage; D043169:Endostatins / Q000008:administration & dosage* / Q000235:genetics; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D010761:Phosphorus Radioisotopes / Q000737:chemistry; D051381:Rats
chemical_list: D003102:Colloids; D043169:Endostatins; D010761:Phosphorus Radioisotopes; C000615311:Phosphorus-32
keywords: endostatin; gene therapy; radiotherapy