title: Investigating the potential for cryopreservation of human granulocytes with concentrated glycerol.
abstract: The purpose of this study was to investigate the potential for cryopreservation of granulocytes using 30% glycerol. Recently reported permeability data was used to design two different methods for addition and removal of glycerol: a fast method that is predicted to keep cell volumes between 80% and 150% of the isotonic volume and a slow method that is predicted to keep cell volumes between 80% and 115% of the isotonic volume. The fast method resulted in cell recoveries of 31% ± 9% and 11% ± 3% before and after freezing, respectively, whereas the slow method resulted in even lower cell recoveries of 5% ± 2% and 4% ± 2%. The reduced cell recovery for the slow method is consistent with an increase in damage as a result of glycerol toxicity. Our results suggest that cryopreservation of granulocytes in concentrated glycerol is not feasible.
authors.affiliation: School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR 97331-2702, USA.
authors.forename: Alden C
authors.initials: AC
authors.lastname: Moss
authors.affiliation: School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR 97331-2702, USA. Electronic address: adam.higgins@oregonstate.edu.
authors.forename: Adam Z
authors.initials: AZ
authors.lastname: Higgins
mesh_terms: D048429:Cell Size; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D006098:Granulocytes; D006801:Humans
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol
keywords: Cryoprotectant; Osmotic damage; Polymorphonuclear cells; Toxicity; Transfusion