title: Acetylated starch nanocrystals: Preparation and antitumor drug delivery study.
abstract: In this study, we developed a new nanoparticulate system for acetylated starch nanocrystals (ASN) using broken rice. ASN with different degrees of substitution (DS) of 0.04, 0.08 and 0.14 were prepared using acetic anhydride as acetylating agent through reaction with starch nanocrystals (SN). The resulting ASN were investigated for the capability to load and release doxorubicin hydrochloride (DOX), and the antitumor activities of DOX-loaded SN and DOX-loaded ASN were evaluated as potential drug delivery systems for cancer therapy. Cellular uptake and cytotoxicity of nanocrystals and the DOX-loaded nanocrystals were investigated using fluorescence microscopy and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Compared with acetylated starches (AS) and native starches (NS), ASN with DS 0.14 loaded up to 6.07% of DOX with a higher loading efficiency of 91.1% and had steadier drug-release rates. Toxicity analysis using the rat hepatocytes model suggested that ASN was biocompatible and could be used for drug delivery. Furthermore, ASN were taken up by cancer cells in vitro and significantly enhanced the cytotoxicity of DOX against HeLa human cervical carcinoma cells. The IC50 value of DOX-loaded ASN-DS 0.14 was 3.8μg/mL for 24h of treatment, which was significantly lower than that of free DOX (21μg/mL). These results indicate that the prepared ASN using broken rice is a promising vehicle for the controlled delivery of DOX for cancer therapy.
authors.affiliation: National Engineering Laboratory for Rice and By-Products Further Processing, Central South University of Forestry & Technology, Changsha 410004, PR China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xiao
authors.affiliation: National Engineering Laboratory for Rice and By-Products Further Processing, Central South University of Forestry & Technology, Changsha 410004, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: National Engineering Laboratory for Rice and By-Products Further Processing, Central South University of Forestry & Technology, Changsha 410004, PR China. Electronic address: linedu2018@163.com.
authors.forename: Qinlu
authors.initials: Q
authors.lastname: Lin
authors.affiliation: National Engineering Laboratory for Rice and By-Products Further Processing, Central South University of Forestry & Technology, Changsha 410004, PR China. Electronic address: gaoliuedu@163.com.
authors.forename: Gao-Qiang
authors.initials: GQ
authors.lastname: Liu
authors.affiliation: National Engineering Laboratory for Rice and By-Products Further Processing, Central South University of Forestry & Technology, Changsha 410004, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Food Science and Technology, Hunan Biological Electromechanical Polytechnic, Changsha 410000, PR China.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Yu
authors.affiliation: National Engineering Laboratory for Rice and By-Products Further Processing, Central South University of Forestry & Technology, Changsha 410004, PR China.
authors.forename: Yuejiao
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000089:Acetic Anhydrides / Q000737:chemistry; D000107:Acetylation; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D006367:HeLa Cells; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy; D051381:Rats; D013213:Starch / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000089:Acetic Anhydrides; D000970:Antineoplastic Agents; C031800:acetic anhydride; D004317:Doxorubicin; D013213:Starch
keywords: Acetylated starch nanoparticles; Broken rice; Drug delivery