title: A β-Lactamase-Imprinted Responsive Hydrogel for the Treatment of Antibiotic-Resistant Bacteria.
abstract: Antibiotics play important roles in infection treatment and prevention. However, the effectiveness of antibiotics is now threatened by the prevalence of drug-resistant bacteria. Furthermore, antibiotic abuse and residues in the environment cause serious health issues. In this study, a stimuli-responsive imprinted hydrogel was fabricated by using β-lactamase produced by bacteria for deactivating antibiotics as the template molecule. The imprinted hydrogel could initially trap β-lactamase excreted by drug-resistant bacteria, thus making bacteria sensitive to antibiotics. After the bactericidal treatment, the "imprinted sites" on the hydrogel could be reversibly abolished with a temperature stimulus, which resulted in the reactivation of β-lactamase to degrade antibiotic residues. We also present an example of the use of this antibacterial design to treat wound infection.
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Dong
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. xqu@ciac.ac.cn.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000627:therapeutic use; D051379:Mice; D054802:Molecular Imprinting / Q000379:methods*; D014946:Wound Infection / Q000188:drug therapy; D001618:beta-Lactamases / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D001618:beta-Lactamases
keywords: antibiotic resistance; antibiotics; enzymes; molecular imprinting; responsive hydrogels