title: Species limits, interspecific hybridization and phylogeny in the cryptic land snail complex Pyramidula: The power of RADseq data.
abstract: Restriction site-associated DNA sequencing (RADseq) was used to jointly assess phylogenetic relationships, interspecific hybridization and species delimitation in the cryptic, non-model land snail complex Pyramidula. A robust phylogeny was inferred using a matrix of concatenated sequences of almost 1,500,000bp long, containing >97,000 polymorphic sites. Maximum likelihood analyses fully resolved the phylogenetic relationships among species and drastically improved phylogenetic trees obtained from mtDNA and nDNA gene trees (COI, 16S rRNA, 5.8S rRNA, ITS2 and 28S rRNA sequence data). The best species delimitation scenario was selected on the basis of 875 unlinked single nucleotide polymorphisms, showing that nine Pyramidula species should be distinguished in Europe. Applying D-statistics provided no or weak evidence of interspecific hybridization among Pyramidula, except for some evidence of gene flow between two species.
authors.affiliation: Department of Zoology and Animal Cell Biology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Alava, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006 Vitoria-Gasteiz, Alava, Spain. Electronic address: oihana.razkin@ehu.es.
authors.forename: Oihana
authors.initials: O
authors.lastname: Razkin
authors.affiliation: Royal Belgian Institute of Natural Sciences (OD Taxonomy and Phylogeny & JEMU), Vautierstraat 29, B-1000 Brussels, Belgium. Electronic address: gontran.sonet@naturalsciences.be.
authors.forename: Gontran
authors.initials: G
authors.lastname: Sonet
authors.affiliation: Royal Belgian Institute of Natural Sciences (OD Taxonomy and Phylogeny & JEMU), Vautierstraat 29, B-1000 Brussels, Belgium. Electronic address: karin.breugelmans@naturalsciences.be.
authors.forename: Karin
authors.initials: K
authors.lastname: Breugelmans
authors.affiliation: Department of Zoology and Animal Cell Biology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Alava, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006 Vitoria-Gasteiz, Alava, Spain. Electronic address: mariajose.madeira@ehu.es.
authors.forename: María José
authors.initials: MJ
authors.lastname: Madeira
authors.affiliation: Department of Zoology and Animal Cell Biology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Alava, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006 Vitoria-Gasteiz, Alava, Spain. Electronic address: benjamin.gomez@ehu.es.
authors.forename: Benjamín Juan
authors.initials: BJ
authors.lastname: Gómez-Moliner
authors.affiliation: Royal Belgian Institute of Natural Sciences (OD Taxonomy and Phylogeny & JEMU), Vautierstraat 29, B-1000 Brussels, Belgium; Evolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. Electronic address: thierry.backeljau@naturalsciences.be.
authors.forename: Thierry
authors.initials: T
authors.lastname: Backeljau
mesh_terms: D000818:Animals; D001483:Base Sequence; D005843:Geography; D006824:Hybridization, Genetic; D016013:Likelihood Functions; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015183:Restriction Mapping / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012908:Snails / Q000145:classification* / Q000235:genetics*; D013045:Species Specificity
keywords: Interspecific hybridization; Phylogeny; Pyramidula; Restriction-site associated DNA sequencing; Species delimitation