title: Silencing of CtBP1 suppresses the migration in human glioma cells.
abstract: Carboxyl-terminal binding protein 1 (CtBP1), up-regulated in various types of human cancers, has been functionally associated with proliferation, anti-apoptosis, and EMT in vitro studies. However, the functional significance of CtBP1 in the pathophysiology of glioma remains unknown. In the present study, we showed the expression of CtBP1 was markedly higher in glioma tissues compared with normal brain tissues by Western blot analysis. Immunohistochemical analysis revealed that CtBP1 mainly localized in the nucleus of glioma cells. Statistical analysis suggested the upregulation of CtBP1 was considerably correlated with the WHO grade (P < 0.05) and those patients with high CtBP1 levels exhibited shorter survival time (P < 0.01). Silencing CtBP1 by short hairpin RNAi caused an inhibition of cell migration. Moreover, knockdown of CtBP1 increases E-cadherin expression and decreases vimentin expression. These data uncovered that CtBP1 protein is a valuable marker of glioma pathogenic process and that CtBP1 can serve as a novel prognostic marker for glioma therapy.
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Chengjin
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Immunology, Medical School of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yifen
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Xuelei
authors.initials: X
authors.lastname: Tao
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Immunology, Medical School of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Immunology, Medical School of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Immunology, Medical School of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu Province, People's Republic of China. taotaont@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.affiliation: Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China. zhangxbnt@163.com.
authors.forename: Xiubing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D005910:Glioma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D004268:DNA-Binding Proteins; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein
keywords: CtBP1; E-cadherin; Glioma; Migration