title: Qualitative and quantitative analysis of the chemical constituents in Mahuang-Fuzi-Xixin decoction based on high performance liquid chromatography combined with time-of-flight mass spectrometry and triple quadrupole mass spectrometers.
abstract: High-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF/MS) and high-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QQQ/MS/MS) were utilized to clarify the chemical constituents of Mahuang-Fuzi-Xixin Decoction. There are 52 compounds, including alkaloids, amino acids and organic acids were identified or tentatively characterized by their characteristic high resolution mass data by HPLC-QQQ/MS/MS. In the subsequent quantitative analysis, 10 constituents, including methyl ephedrine, aconine, songrine, fuziline, neoline, talatisamine, chasmanine, benzoylmesaconine, benzoylaconine and benzoylhypaconine were simultaneously determined by HPLC-QQQ/MS/MS with multiple reaction monitoring mode. Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r > 0.9992). The relative standard deviations (RSD) of inter- and intra-day precisions were <3%. This method was also validated by repeatability, stability and recovery with RSD <3% respectively. A highly sensitive and efficient method was established for chemical constituents studying, including identification and quantification of Mahuang-Fuzi-Xixin decoction.
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Cao
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.|Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Haiqiang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Gong
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China. rosierong@163.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Rong
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D030023:Asarum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D029790:Ephedra sinica / Q000737:chemistry*; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D000596:Amino Acids; D004365:Drugs, Chinese Herbal
keywords: HPLC-QQQ/MS/MS; HPLC-TOF/MS; Mahuang-Fuzi-Xixin decoction; qualitative analysis; quantitative analysis