title: In Vivo (19)F-Magnetic Resonance Imaging of Adoptively Transferred NK Cells.
abstract: In order to assess the biodistribution, homing, and persistence of adoptively transferred natural killer (NK) cell immunotherapies, there is a need for imaging methodology suitable for use in preclinical studies with relevance to clinical translation. Amongst the available approaches, (19)F-MRI is very appealing for in vivo imaging due to the absence of background signal, enabling clear detection of (19)F labeled cells in vivo. Here we describe a methodology for in vivo imaging of adoptively transferred NK cells labeled with (19)F nano-emulsion, using clinically translatable technology of (19)F/(1)H magnetic resonance imaging.
authors.affiliation: Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, 77054, USA.
authors.forename: Srinivas S
authors.initials: SS
authors.lastname: Somanchi
authors.affiliation: Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, 77054, USA.
authors.forename: Bridget A
authors.initials: BA
authors.lastname: Kennis
authors.affiliation: Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, 77054, USA.
authors.forename: Vidya
authors.initials: V
authors.lastname: Gopalakrishnan
authors.affiliation: Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, 77054, USA.
authors.forename: Dean A
authors.initials: DA
authors.lastname: Lee
authors.affiliation: Department of Imaging Physics, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX, 77054, USA. jbankson@mdanderson.org.
authors.forename: James A
authors.initials: JA
authors.lastname: Bankson
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000628:therapy*; D002452:Cell Count; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D066235:Fluorine-19 Magnetic Resonance Imaging / Q000379:methods*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000637:transplantation*; D051379:Mice; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
keywords: 19F; Adoptive immunotherapy; In vivo imaging; Magnetic resonance imaging; NK cells