title: Involvement of CmWRKY10 in Drought Tolerance of Chrysanthemum through the ABA-Signaling Pathway.
abstract: Drought is one of the important abiotic factors that adversely affects plant growth and production. The WRKY transcription factor plays a pivotal role in plant growth and development, as well as in the elevation of many abiotic stresses. Among three major groups of the WRKY family, the group IIe WRKY has been the least studied in floral crops. Here, we report functional aspects of group IIe WRKY member, i.e., CmWRKY10 in chrysanthemum involved in drought tolerance. The transactivation assay showed that CmWRKY10 had transcriptional activity in yeast cells and subcellular localization demonstrated that it was localized in nucleus. Our previous study showed that CmWRKY10 could be induced by drought in chrysanthemum. Moreover, the overexpression of CmWRKY10 in transgenic chrysanthemum plants improved tolerance to drought stress compared to wild-type (WT). High expression of DREB1A, DREB2A, CuZnSOD, NCED3A, and NCED3B transcripts in overexpressed plants provided strong evidence that drought tolerance mechanism was associated with abscisic acid (ABA) pathway. In addition, lower accumulation of reactive oxygen species (ROS) and higher enzymatic activity of peroxidase, superoxide dismutase and catalase in CmWRKY10 overexpressed lines than that of WT demonstrates its role in drought tolerance. Together, these findings reveal that CmWRKY10 works as a positive regulator in drought stress by regulating stress-related genes.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. abuzar_jaffar@yahoo.com.
authors.forename: Muhammad Abuzar
authors.initials: MA
authors.lastname: Jaffar
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. aiping_song@aliyun.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China. mlofaheem@gmail.com.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Faheem
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. jiangjiafu@njau.edu.cn.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2014204030@njau.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2013104100@njau.edu.cn.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Fan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chenfd@njau.edu.cn.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D031202:Chrysanthemum / Q000235:genetics / Q000378:metabolism*; D055864:Droughts; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D000040:Abscisic Acid; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: WRKY; abscisic acid; chrysanthemum; drought