title: Complete genome sequence of Vibrio parahaemolyticus strain FORC_008, a foodborne pathogen from a flounder fish in South Korea.
abstract: Vibrio parahaemolyticus is a Gram-negative, motile, nonspore-forming pathogen that causes foodborne illness associated with the consumption of contaminated seafoods. Although many cases of foodborne outbreaks caused by V. parahaemolyticus have been reported, the genomes of only five strains have been completely sequenced and analyzed using bioinformatics. In order to characterize overall virulence factors and pathogenesis of V. parahaemolyticus associated with foodborne outbreak in South Korea, a new strain FORC_008 was isolated from flounder fish and its genome was completely sequenced. The genomic analysis revealed that the genome of FORC_008 consists of two circular DNA chromosomes of 3266 132 bp (chromosome I) and 1772 036 bp (chromosome II) with a GC content of 45.36% and 45.53%, respectively. The entire genome contains 4494 predicted open reading frames, 129 tRNAs and 31 rRNA genes. While the strain FORC_008 does not have genes encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), its genome encodes many other virulence factors including hemolysins, pathogenesis-associated secretion systems and iron acquisition systems, suggesting that it may be a potential pathogen. This report provides an extended understanding on V. parahaemolyticus in genomic level and would be helpful for rapid detection, epidemiological investigation and prevention of foodborne outbreak in South Korea.
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Suyeon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Han Young
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Jong Gyu
authors.initials: JG
authors.lastname: Lim
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Se Keun
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Ku
authors.affiliation: Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: You-Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
authors.affiliation: Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea juhlee@khu.ac.kr.
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D005399:Fishes / Q000821:virology*; D016679:Genome, Viral; D023281:Genomics / Q000379:methods; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014735:Vibrio Infections / Q000821:virology; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors
keywords: Vibrio parahaemolyticus; complete genome sequence; foodborne pathogen; foodborne pathogen omics research center; gastroenteritis; seafood