title: Functionalizing the glycocalyx of living cells with supramolecular guest ligands for cucurbit[8]uril-mediated assembly.
abstract: Multiple naphthol ligands were installed on the glycocalyx of white blood cells via metabolic labeling and subsequent strain promoted azide-alkyne cycloaddition. Only when cucurbit[8]uril was present to drive the formation of ternary complexes, cells specifically assembled on a methylviologen functionalized supported lipid bilayer through multivalent interactions.
authors.affiliation: Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, Department of Science and Technology, University of Twente, P. O. Box 217, 7500 AE, Enschede, The Netherlands. j.huskens@utwente.nl p.jonkheijm@utwente.nl.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Cavatorta
authors.forename: Mark L
authors.initials: ML
authors.lastname: Verheijden
authors.forename: Wies
authors.initials: W
authors.lastname: van Roosmalen
authors.forename: Jens
authors.initials: J
authors.lastname: Voskuhl
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Huskens
authors.forename: Pascal
authors.initials: P
authors.lastname: Jonkheijm
mesh_terms: D000480:Alkynes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D001386:Azides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D019276:Glycocalyx / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism*; D019169:Jurkat Cells; D007962:Leukocytes / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D009284:Naphthols / Q000737:chemistry / Q000378:metabolism*; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D000480:Alkynes; D001386:Azides; D001952:Bridged-Ring Compounds; D007093:Imidazoles; D008024:Ligands; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D009284:Naphthols; C507198:cucurbit(8)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils