title: PO-17 - Identification of IgG bound to plasminogen in oncologic diseases.
abstract: BACKGROUND
  The binding of plasminogen (Pg) to cell receptors and extracellular ligands facilitates its activation to plasmin, which stimulates the extracellular matrix degradation, neoangiogenesis and tumor invasion. Plasmin can also degrade IgG thereby exposing C-terminal lysine residues. Previously, we have found IgG specifically bounded to Pg in the plasma of patients with malignant tumors.
  
  
  OBJECTIVE
  To identify IgG degraded by plasmin in the plasma of cancer patients.
  
  
  METHODS
  Methods of ELISA were used for comparative research of levels of IgG bound to Pg in plasma of patients with the prostate cancer (PC, n=25) and lung cancer (LC, n=17). Plasma of healthy donors (n=29) was used as control. All patients signed informed consent for participation in this study. Affinity chromatography on Pg-sepharose was used for the quantification of IgG. Carboxypeptidase was used for remove of C-terminal lysine residues of the IgG. The program ATTESTAT was used for nonparametric analysis.
  
  
  RESULTS
  The frequency of occurence of elevated levels of IgG to Pg in plasma was detected in 68% of patients with PC, 59% of patients with LC and only 12% of healthy women and 10% of healthy men. The quantification of antibodies in plasma samples showed that the quantity of IgG to Pg in patients with PC was 27% from the total amount of IgG and in healthy men - 9%. Treatment of diluted plasma samples with carboxypeptidase B abolished the elevated levels of IgG to Pg, as well as the specific activity of the purified IgG to Pg-sepharose.
  
  
  CONCLUSIONS
  C-terminal lysine residues which are formed as a result of degradation of native IgG with plasmin can bind to lysine binding sites on the kringle domains of Pg. Increased levels of these degraded IgG can be marker at cancer.
authors.affiliation: Angiogen Co. Ltd.
authors.forename: V N
authors.initials: VN
authors.lastname: Iakovlev
authors.affiliation: Institute of Human Morphology, Russian Academy of Medical Sciences.
authors.forename: E I
authors.initials: EI
authors.lastname: Goufman
authors.affiliation: Institute of Human Morphology, Russian Academy of Medical Sciences.
authors.forename: N B
authors.initials: NB
authors.lastname: Tikhonova
authors.affiliation: Lomonosov Moscow State University, Chemistry Faculty.
authors.forename: R B
authors.initials: RB
authors.lastname: Aisina
authors.affiliation: Lomonosov Moscow State University, Chemistry Faculty.
authors.forename: L I
authors.initials: LI
authors.lastname: Mukhametova
authors.affiliation: Emanuel Institute of Biochemical Physics of Russian Academy of Sciences; Russia.
authors.forename: K B
authors.initials: KB
authors.lastname: Gershkovich