title: Traditional and Modern Cell Culture in Virus Diagnosis.
abstract: Cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. With the recent advances in technology, cell culture is considered a gold standard for virus isolation. This paper reviews the evolution of cell culture methods and demonstrates why cell culture is a preferred method for identification of viruses. In addition, the advantages and disadvantages of both traditional and modern cell culture methods for diagnosis of each type of virus are discussed. Detection of viruses by the novel cell culture methods is considered more accurate and sensitive. However, there is a need to include some more accurate methods such as molecular methods in cell culture for precise identification of viruses.
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Hematian
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Nourkhoda
authors.initials: N
authors.lastname: Sadeghifard
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mohebi
authors.affiliation: Razi Herbal Medicines Research Center and Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Morovat
authors.initials: M
authors.lastname: Taherikalani
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Nasrolahi
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Mansour
authors.initials: M
authors.lastname: Amraei
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Sobhan
authors.initials: S
authors.lastname: Ghafourian
keywords: cell culture; gold standard; traditional culture; viruses