title: In Vivo Inhibition of Porphyromonas gingivalis Growth and Prevention of Periodontitis With Quorum-Sensing Inhibitors.
abstract: Autoinducer (AI)-2 has an important role in biofilm formation in the oral environment. Mature biofilms formed as a result of the cell-to-cell communication make it difficult to overcome periodontitis with the use of antibiotics. Previous in vitro studies suggest that quorum-sensing inhibitors (QSIs) interfere with AI-2. This study compares the QSI effects resulting from an oral inoculation of Porphyromonas gingivalis in an experimental animal model.
  
  
  
  Forty-five male mice were divided into three groups (n = 15 each): 1) infection; 2) QSI; and 3) control. Infection and QSI groups received oral inoculation of P. gingivalis, whereas treatment with QSIs (furane compound and d-ribose) was only performed in the QSIs group. The control group was a negative control not receiving manipulation. After 42 days, mice were sacrificed, and the distance from the alveolar bone crest (ABC) to the cemento-enamel junction (CEJ) was measured by microcomputed tomography. P. gingivalis DNA was quantified in the soft and hard tissues around the molar teeth by real-time polymerase chain reaction.
  
  
  
  Distance from ABC to CEJ was significantly increased in the P. gingivalis infection group compared with the control group (P = 0.02) and significantly decreased in the QSI group compared with the infection group (P = 0.02). The QSI group contained 31.64% of the bacterial DNA count of the infection group.
  
  
  
  Use of QSIs in the mice infection model showed a reduction of bone breakdown and a decrease in the number of bacteria in vivo, suggesting that QSIs can be a new approach to prevention and treatment of periodontitis.
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Young-Jae
authors.initials: YJ
authors.lastname: Cho
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hyun Young
authors.initials: HY
authors.lastname: Song
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Heithem
authors.initials: H
authors.lastname: Ben Amara
authors.affiliation: Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University.
authors.forename: Bong-Kyu
authors.initials: BK
authors.lastname: Choi
authors.affiliation: Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University.
authors.forename: Ryu
authors.initials: R
authors.lastname: Eunju
authors.affiliation: Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Young-Ah
authors.initials: YA
authors.lastname: Cho
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yangjo
authors.initials: Y
authors.lastname: Seol
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yongmoo
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Ku
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Rhyu
authors.affiliation: Department of Periodontology and Dental Research Institute, Translational Research Laboratory for Tissue Engineering, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Koo
mesh_terms: D016301:Alveolar Bone Loss; D000818:Animals; D008297:Male; D051379:Mice; D010518:Periodontitis / Q000517:prevention & control*; D016966:Porphyromonas gingivalis / Q000254:growth & development*; D053038:Quorum Sensing / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction; D055114:X-Ray Microtomography
keywords: Biofilms; Porphyromonas gingivalis; models, animal; quorum sensing; ribose