title: Methylation of miR-145a-5p promoter mediates adipocytes differentiation.
abstract: MicroRNAs (miRNAs, miR) play important roles in adipocyte development. Recent studies showed that the expression of several miRNAs is closely related with promoter methylation. However, it is not known whether miRNA mediates adipocytes differentiation by means of DNA methylation. Here, we showed that miR-145a-5p was poorly expressed in adipose tissue from mice fed a high fat diet (HFD). Overexpression or inhibition of miR-145a-5p was unfavorable or beneficial, respectively, for adipogenesis, and these effects were achieved by regulating adipocyte-specific genes involved in lipogenic transcription, fatty acid synthesis, and fatty acid transportation. Particularly, we first suggested that miR-145a-5p mimics or inhibitors promoted or repressed adipocytes proliferation by regulating p53 and p21, which act as cell cycle regulating factors. Surprisingly, the miR-145a-5p-repressed adipocyte differentiation was enhanced or rescued when cells treated with 5-Aza-dC were transfected with miR-145a-5p mimics or inhibitors, respectively. These data indicated that, as a new mean to positively regulate adipocyte proliferation, the process of miR-145a-5p-inhibited adipogenesis may be regulated by DNA methylation.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Du
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Cheng
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Linyuan
authors.initials: L
authors.lastname: Shen
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Tan
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Chendong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Animal Husbandry and Veterinary Medicine, Chengdu Agricultural College, Chengdu 611100, Sichuan, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: College of Life and Science, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: zhangsh1919@163.com.
authors.forename: Shunhua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: zhuli7508@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D019175:DNA Methylation; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: C543499:MIRN145a microRNA, mouse; D035683:MicroRNAs
keywords: DNA methylation; Differentiation; Proliferation; miR-145a-5p