title: Transplantation of human adipose-derived mesenchymal stem cells on a bladder acellular matrix for bladder regeneration in a canine model.
abstract: Tissue engineering brings new hope for the reconstruction of injured bladders. The aim of the present study was to evaluate human adipose-derived mesenchymal stem cells (hADSCs) combined with a bladder acellular matrix (BAM) for bladder regeneration. A BAM or BAM loaded with hADSCs (BAM/hADSCs) was used to repair partial cystectomy of the bladder in a canine model. 6 months after implantation, calculi and urine leakage were not found in either the BAM or BAM/hADSCs group by cystography. And compared to the BAM group, a significant increase of maximum bladder volume and bladder compliance was observed in the BAM/hADSCs group by urodynamics evaluation. Moreover, histological analysis showed that the BAM/hADSCs group could more effectively promote the regeneration of bladder smooth muscle and vascularization than the BAM group. These results demonstrated that a BAM/hADSCs could be an effective approach to promote bladder reconstruction with potential clinical applications.
authors.affiliation: Clinical and Translational Research Center of Shanghai First Maternity and Infant Health Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100190, People's Republic of China. Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Road, Chongqing 400038, People's Republic of China.
authors.forename: Xianglin
authors.initials: X
authors.lastname: Hou
authors.forename: Chunying
authors.initials: C
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Weisheng
authors.initials: W
authors.lastname: Jia
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Gang
authors.initials: G
authors.lastname: Ye
authors.forename: Jiuhong
authors.initials: J
authors.lastname: Kang
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D003094:Collagen; D004285:Dogs; D005109:Extracellular Matrix; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010641:Phenotype; D019651:Plastic Surgery Procedures / Q000379:methods*; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D001743:Urinary Bladder / Q000601:surgery*
chemical_list: D003094:Collagen