title: Simultaneous determination of 14 antiviral drugs and relevant metabolites in chicken muscle by UPLC-MS/MS after QuEChERS preparation.
abstract: A fast method for the simultaneous determination of 14 antiviral drugs and relevant metabolites in chicken muscle by ultra-high liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed. The analytes included anti-influenza drugs (amantadine, rimantadine, oseltamivir, oseltamivir carboxylate, memantine, arbidol, and moroxydine), anti-herpes drugs (acyclovir, ganciclovir, famciclovir, penciclovir, ribavirin and its main metabolite TCONH2), and an immunomodulator (imiquimod). The samples were pretreated using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. The determination of the target compounds was conducted in less than 11.0min, and specificity was ensured by the use of multiple reaction monitoring (MRM) acquisition mode. Good linearities (R(2)>0.9928) were obtained in the range of 0.1-100μg/L, and the recovery rates were 56.2-113.4% with RSDs of 1.7-10.3% for intra-day precision and 2.4-8.8% for inter-day precision. The LODs and LOQs of all analytes were in the ranges of 0.02-1.0 and 0.05-2.5μg/kg, respectively. An analysis of real samples suggested that this method is simple, sensitive, reliable and practical for the detection of antiviral drugs in animal tissue.
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Pengqian
authors.initials: P
authors.lastname: Mu
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Xu
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Chai
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Shuming
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China. Electronic address: qianyongzhong@caas.cn.
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China. Electronic address: qiujing@caas.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000998:Antiviral Agents
keywords: Antiviral drugs; Chicken muscle; Metabolites; QuEChERS method; UPLC–MS/MS