title: The chemical toxicity of cesium in Indian mustard (Brassica juncea L.) seedlings.
abstract: To distinguish between the radiological and chemical effects of radiocesium, we study the chemical toxicity of cesium in the seedlings of Indian mustard (Brassica juncea L.). In this study, the experiment was designed in two factors and five levels random block design to investigate the interaction effects of Cs and K. Results showed that excessive Cs was one of the main factors influence the growth of Brassica juncea seedlings. And the toxicity of Cs in Brassica juncea is likely to be caused by Cs interacts with K-binding sites in essential K-dependent protein, either competes with K for essential biochemical functions, causing intracellular metabolic disturbance. To test the hypothesis that the toxicity of Cs might cause intracellular metabolic disturbance, next-generation sequencing (NGS)-based Illumina paired-end Solexa sequencing platform was employed to analysis the changes in gene expression, and understand the key genes in B. juncea seedlings responding to the toxicity of Cs. Based on the assembled de novo transcriptome, 2032 DEGs that play significant roles in the response to the toxicity of Cs were identified. Further analysis showed that excessive Cs is disturbance the auxin signal transduction pathway, and inhibited the indoleacetic acid-induced protein (AUX/IAA) genes expression eventually lead the seedlings growth and development be inhibited. The results suggest that disturbances to tryptophan metabolism might be linked to changes in growth.
authors.affiliation: Life Science College, Sichuan Normal University, Chengdu, Sichuan, 610101, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Lai
authors.affiliation: Life Science College, Sichuan Normal University, Chengdu, Sichuan, 610101, China. Electronic address: t89807596@163.com.
authors.forename: Zong-Ya
authors.initials: ZY
authors.lastname: Tao
authors.affiliation: Life Science College, Sichuan Normal University, Chengdu, Sichuan, 610101, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Life Science College, Sichuan Normal University, Chengdu, Sichuan, 610101, China.
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.affiliation: Life Science College, Sichuan Normal University, Chengdu, Sichuan, 610101, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Wu
authors.affiliation: Life Science College, Sichuan Normal University, Chengdu, Sichuan, 610101, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Life Science College, Sichuan Normal University, Chengdu, Sichuan, 610101, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.affiliation: Engineering Research Center of Biomass Materials (SWUST), Ministry of Education, Mianyang, 621010, China.
authors.forename: Xue-Gang
authors.initials: XG
authors.lastname: Luo
mesh_terms: D002586:Cesium / Q000493:pharmacokinetics / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism; D009149:Mustard Plant / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D007210:Indoleacetic Acids; D002586:Cesium; C030737:indoleacetic acid; D011188:Potassium
keywords: Cesium; Differentially expressed genes (DEGs); Indian mustard (Brassica juncea L.); Toxicity