title: Calcium influx through TRP channels induced by short-lived reactive species in plasma-irradiated solution.
abstract: Non-equilibrium helium atmospheric-pressure plasma (He-APP), which allows for a strong non-equilibrium chemical reaction of O2 and N2 in ambient air, uniquely produces multiple extremely reactive products, such as reactive oxygen species (ROS), in plasma-irradiated solution. We herein show that relatively short-lived unclassified reactive species (i.e., deactivated within approximately 10 min) generated by the He-APP irradiation can trigger physiologically relevant Ca(2+) influx through ruthenium red- and SKF 96365-sensitive Ca(2+)-permeable channel(s), possibly transient receptor potential channel family member(s). Our results provide novel insight into understanding of the interactions between cells and plasmas and the mechanism by which cells detect plasma-induced chemically reactive species, in addition to facilitating development of plasma applications in medicine.
authors.affiliation: Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Sasaki
authors.affiliation: Graduate School of Biomedical Engineering, Tohoku University, 6-6-04 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kanzaki
authors.affiliation: Department of Electronic Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Kaneko
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001274:Atmospheric Pressure; D002118:Calcium / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006371:Helium / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007093:Imidazoles / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D058626:Plasma Gases / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012430:Ruthenium Red / Q000378:metabolism; D059010:Single-Cell Analysis; D012996:Solutions; D013997:Time Factors; D059008:Time-Lapse Imaging; D050051:Transient Receptor Potential Channels / Q000378:metabolism*
chemical_list: D007093:Imidazoles; D058626:Plasma Gases; D017382:Reactive Oxygen Species; D012996:Solutions; D050051:Transient Receptor Potential Channels; D012430:Ruthenium Red; D006371:Helium; D017665:Hydroxyl Radical; D008353:Mannitol; D006861:Hydrogen Peroxide; C063159:1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole; D005947:Glucose; D002118:Calcium