title: KHF16 is a Leading Structure from Cimicifuga foetida that Suppresses Breast Cancer Partially by Inhibiting the NF-κB Signaling Pathway.
abstract: Triterpenoids extracted from Cimicifuga foetida have been reported to inhibit cancer by inducing cell cycle arrest and apoptosis. In this study, KHF16 (24-acetylisodahurinol-3-O-β-D-xylopyranoside), a cycloartane triterpenoid isolated from the rhizomes of C. foetida, showed potent anti-cancer activity in multiple ERα/PR/HER2 triple-negative breast cancer (TNBC) cell lines. KHF16 significantly induces cell cycle G2/M phase arrest and apoptosis in both MDA-MB-468 and SW527 TNBC cell lines. KHF16 reduces the expression levels of XIAP, Mcl-1, Survivin and Cyclin B1/D1 proteins. Importantly, KHF16 inhibits TNFα-induced IKKα/β phosphorylation, IKBα phosphorylation, p65 nuclear translocation and NF-κB downstream target gene expression, including XIAP, Mcl-1 and Survivin, in TNBC cells. These results suggest that KHF16 may inhibit TNBC by blocking the NF-κB signaling pathway in part.
authors.affiliation: 1. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China;; 2. Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650204 Yunnan, China;
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Kong
authors.affiliation: 1. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China;; 2. Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650204 Yunnan, China;
authors.forename: Fubin
authors.initials: F
authors.lastname: Li
authors.affiliation: 1. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China;; 3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650204 Yunnan, China;
authors.forename: Yin
authors.initials: Y
authors.lastname: Nian
authors.affiliation: 1. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China;
authors.forename: Zhongmei
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: 4. Department of the second medical oncology, the 3rd affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650118, China.
authors.forename: Runxiang
authors.initials: R
authors.lastname: Yang
authors.affiliation: 3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650204 Yunnan, China;
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Qiu
authors.affiliation: 1. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China;
authors.forename: Ceshi
authors.initials: C
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D031885:Cimicifuga / Q000737:chemistry*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D064726:Triple Negative Breast Neoplasms / Q000188:drug therapy*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D064209:Phytochemicals; D010936:Plant Extracts; D014315:Triterpenes; C085352:cycloartane
keywords: Apoptosis; Cell cycle; Cycloartane triterpenoid; KHF16; NF-κB.; Triple negative breast cancer