title: Characterization of hemolysins of Staphylococcus strains isolated from human and bovine, southern Iran.
abstract: The staphylococci are important pathogenic bacteria causing various infections in animals and human. Hemolysin is one of the virulence factors of coagulase-positive (CPS) and coagulase-negative staphylococci (CNS). The aims of the study were to characterize hemolysins of Staphylococcus spp. isolated from human and bovine origin, phenotypic- and genotypically. Characterization of hemolysin phenotypically based on hemolysis pattern of Staphylococcus spp. was done on the sheep, horse and rabbit blood agar plates. Genes encoding hemolysin were amplified with specific primers by using polymerase chain reaction (PCR) technique. Hemolytic activities phenotypically were determined in 60 and 90% of the total bovine and human isolates, respectively. All non hemolytic isolates were CNS (P≤0.05). In all isolates, hla and hld genes were determined by PCR amplification. None of the bovine and human isolates showed phenotypically and genotypically gamma hemolysin. The results from this study suggest that, in accordance with what is generally believed, some differences are apparent in hemolysin types among Staphylococcus strains of bovine and human origin. Furthermore, this study showed that CNS can be important as new pathogens.
authors.affiliation: DVM Student, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;
authors.forename: Z
authors.initials: Z
authors.lastname: Moraveji
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine and Institute of Biotechnology, Shiraz University, Shiraz, Iran;
authors.forename: M
authors.initials: M
authors.lastname: Tabatabaei
authors.affiliation: Ph.D. Student in Bacteriology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;
authors.forename: H
authors.initials: H
authors.lastname: Shirzad Aski
authors.affiliation: Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Khoshbakht
keywords: Hemolysin; Human and bovine isolates; PCR; Staphylococcus spp.