title: Molecular epidemiology and antifungal susceptibility of Saprochaete capitata (Blastoschizomyces capitatus) isolates causing nosocomial infection in Kayseri/Turkey.
abstract: BACKGROUND
  Saprochaete capitata isolates have emerged as important nosocomial pathogens, among immunosuppressed or neutropenic patients, and a rare cause of nosocomial infection in the hematology-bone marrow unit (HBMU) and the intensive care unit (ICU). The purpose of this study was to molecular epidemiology and antifungal susceptibility of S. capitata (Blastoschizomyces capitatus) isolates causing nosocomial infection at Kayseri in Turkey.
  
  
  METHODS
  During a period from 2012 to 2015, a total of 20 S. capitata strains were obtained from patients hospitalized at Erciyes University Hospital. The identification of S. capitata was performed by phenotypic and biochemical methods; this was confirmed by molecular methods by DNA sequencing analysis. Genotyping of S.capitata isolates from different patients was determined to by the repetitive sequence PCR (repPCR) using the DiversiLab System (BioMerieux).
  
  
  RESULTS
  More than half of the patients with S. capitata infections were hospitalized in the hematology-oncology unit (60%). The patients mainly included those using intravascular devices (90%), and receiving parenteral antibiotics (85%); the mortality rate was 55%. The microbiological investigation failed to identify S. capitata in the hospital environment. All isolates were resistant to caspofungin (>32). However, the MIC90 values for voriconazole, amphotericin B, and fluconazole against all of the isolates were 0.125, 0.25, and 1μg/ml, respectively. The S. capitata strains belonged to five clones (A-E) which were determined by the use of rep-PCR and Clone C was found to be predominant.
  
  
  CONCLUSIONS
  S. capitata isolates are an important cause of nosocomial infection in the HBMU and ICUs.
authors.affiliation: a Department of Medical Microbiology , Faculty of Medicine, Erciyes University , Kayseri , Turkey ;
authors.forename: A Nedret
authors.initials: AN
authors.lastname: Koç
authors.affiliation: a Department of Medical Microbiology , Faculty of Medicine, Erciyes University , Kayseri , Turkey ;
authors.forename: M Altay
authors.initials: MA
authors.lastname: Atalay
authors.affiliation: a Department of Medical Microbiology , Faculty of Medicine, Erciyes University , Kayseri , Turkey ;
authors.forename: Demet
authors.initials: D
authors.lastname: Timur
authors.affiliation: a Department of Medical Microbiology , Faculty of Medicine, Erciyes University , Kayseri , Turkey ;
authors.forename: Gonca
authors.initials: G
authors.lastname: Demir
authors.affiliation: b Department of Hematology and Oncology , Faculty of Medicine, Erciyes University , Kayseri , Turkey.
authors.forename: Leylagül
authors.initials: L
authors.lastname: Kaynar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009181:Mycoses / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D004718:Saccharomycetales / Q000187:drug effects* / Q000235:genetics*; D014421:Turkey / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000935:Antifungal Agents
keywords: Antifungal susceptibility; DNA sequencing analysis; Saprochaete capitata; genotyping; nosocomial fungal infection; repetitive sequence PCR by a DiversiLab System