title: Histone H2B ubiquitylation represses gametogenesis by opposing RSC-dependent chromatin remodeling at the ste11 master regulator locus.
abstract: In fission yeast, the ste11 gene encodes the master regulator initiating the switch from vegetative growth to gametogenesis. In a previous paper, we showed that the methylation of H3K4 and consequent promoter nucleosome deacetylation repress ste11 induction and cell differentiation (Materne et al., 2015) but the regulatory steps remain poorly understood. Here we report a genetic screen that highlighted H2B deubiquitylation and the RSC remodeling complex as activators of ste11 expression. Mechanistic analyses revealed more complex, opposite roles of H2Bubi at the promoter where it represses expression, and over the transcribed region where it sustains it. By promoting H3K4 methylation at the promoter, H2Bubi initiates the deacetylation process, which decreases chromatin remodeling by RSC. Upon induction, this process is reversed and efficient NDR (nucleosome depleted region) formation leads to high expression. Therefore, H2Bubi represses gametogenesis by opposing the recruitment of RSC at the promoter of the master regulator ste11 gene.
authors.affiliation: URPHYM-GEMO, Namur Research College, University of Namur, Namur, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Materne
authors.affiliation: Instituto de Biología Funcional y Genómica, Salamanca, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Vázquez
authors.affiliation: Instituto de Biología Funcional y Genómica, Salamanca, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Sánchez
authors.affiliation: URPHYM-GEMO, Namur Research College, University of Namur, Namur, Belgium.
authors.forename: Carlo
authors.initials: C
authors.lastname: Yague-Sanz
authors.affiliation: URPHYM-GEMO, Namur Research College, University of Namur, Namur, Belgium.
authors.forename: Jayamani
authors.initials: J
authors.lastname: Anandhakumar
authors.affiliation: URPHYM-GEMO, Namur Research College, University of Namur, Namur, Belgium.
authors.forename: Valerie
authors.initials: V
authors.lastname: Migeot
authors.affiliation: Instituto de Biología Funcional y Genómica, Salamanca, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Antequera
authors.affiliation: URPHYM-GEMO, Namur Research College, University of Namur, Namur, Belgium.
authors.forename: Damien
authors.identifier: 0000-0002-1029-5848
authors.initials: D
authors.lastname: Hermand
mesh_terms: D042002:Chromatin Assembly and Disassembly; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics*; D014157:Transcription Factors / Q000037:antagonists & inhibitors*; D054875:Ubiquitination
chemical_list: D006657:Histones; D014157:Transcription Factors; D020930:MAP Kinase Kinase Kinases
keywords: Chromatin; Fission yeast; S. pombe; Transcription; chromosomes; genes