title: TP53 Staining in Tissue Samples of Chronic Lymphocytic Lymphoma Cases: An Immunohistochemical Survey of 51 Cases.
abstract: OBJECTIVE
  Chronic lymphocytic leukemia (CLL) is the most common lymphoproliferative disease in adults. The aim of this study is to find out if the extent of proliferation centers or the immunohistochemical expression of p53 is related to disease prognosis.
  
  
  METHODS
  In the scope of this study, 54 biopsy specimens from 51 patients (50 of lymph nodes; the others of spleen, tonsil, orbit, and liver) diagnosed with CLL at the Hacettepe University Department of Pathology in 2000-2013 were reevaluated. The clinical and demographic data of the patients were obtained from our patient database. Biopsy samples were assessed semi-quantitatively for the percentage of proliferation center/total biopsy area (PC/TBA) and an immunohistochemical study was performed on representative blocks of tissues for p53 expression.
  
  
  RESULTS
  When the patients were divided into two categories according to Rai stage as high and low (stages 0, 1, and 2 vs. stages 3 and 4), it was seen that patients with low Rai stage had a better prognosis than those with high stages (p=0.030). However, there was no statistically significant correlation between overall survival and PC/TBA ratio or p53 expression levels.
  
  
  CONCLUSIONS
  In our cohort, PC/TBA ratio and immunopositivity of p53 did not show correlations with overall survival.
authors.affiliation: Johns Hopkins University Faculty of Medicine, Baltimore, USA Phone: +1 410 502 7354 E-mail: dribrahimkulac@gmail.com.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Kulaç
authors.forename: Çetin
authors.initials: Ç
authors.lastname: Demir
authors.forename: Yahya
authors.initials: Y
authors.lastname: Büyükaşık
authors.forename: Tezer
authors.initials: T
authors.lastname: Kutluk
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Üner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53