title: Development of polyamide-6,6/chitosan electrospun hybrid nanofibrous scaffolds for tissue engineering application.
abstract: The development of biofunctional and bioactive hybrid polymeric scaffolds seek to mitigate the current challenges in the emerging field of tissue engineering. In this paper, we report the fabrication of a biomimetic and biocompatible nanofibrous scaffolds of polyamide-6,6 (PA-6,6) blended with biopolymer chitosan via one step co-electrospinning technique. Different weight percentage of chitosan 10wt%, 15wt%, and 20wt% were blended with PA-6,6, respectively. The nanocomposite electrospun scaffolds mats enabled to provide the osteophilic environment for cells growth and biomineralization. The morphological and physiochemical properties of the resulted scaffolds were studied using field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), and Fourier transform-infrared (FT-IR) spectroscopy. The improvement in hydrophilicity and mechanical strength of the bio-nanocomposite mesh with 20wt% chitosan embedded, was the desired avenue for adhesion, proliferation and maturation of osteoblast cells as compared to other sample groups and pure PA-6,6 fibrous mat. The biomineralization of the nanocomposite electrospun mats also showed higher ability to nucleate bioactive calcium phosphate (Ca/P) nanoparticles comparing to pristine PA-6,6. Furthermore, the biomimetic nature of scaffolds exhibited the cells viability and regeneration of pre-osteoblast (MC3T3-E1) cells which were assessed via in vitro cell culture test. Collectively, the results suggested that the optimized 20wt% of chitosan supplemented hybrid electrospun fibrous scaffold has significant effect in biomedical field to create osteogenic capabilities for tissue engineering.
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Bishnu Kumar
authors.initials: BK
authors.lastname: Shrestha
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Engineering Materials and Mechanical Design, Faculty of Engineering, South Valley University, Qena 83523, Egypt.
authors.forename: Hamouda M
authors.initials: HM
authors.lastname: Mousa
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Arjun Prasad
authors.initials: AP
authors.lastname: Tiwari
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Ko
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: biochan@jbnu.ac.kr.
authors.forename: Chan Hee
authors.initials: CH
authors.lastname: Park
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: chskim@jbnu.ac.kr.
authors.forename: Cheol Sang
authors.initials: CS
authors.lastname: Kim
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009757:Nylons / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D009757:Nylons; D048271:Chitosan
keywords: Biocompatibility; Bone tissue engineering; Chitosan; Electrospinning; Nanofiber; Polyamide-6,6