title: Acute and Chronic Electroconvulsive Seizures (ECS) Differentially Regulate the Expression of Epigenetic Machinery in the Adult Rat Hippocampus.
abstract: BACKGROUND
  Electroconvulsive seizure treatment is a fast-acting antidepressant therapy that evokes rapid transcriptional, neurogenic, and behavioral changes. Epigenetic mechanisms contribute to altered gene regulation, which underlies the neurogenic and behavioral effects of electroconvulsive seizure. We hypothesized that electroconvulsive seizure may modulate the expression of epigenetic machinery, thus establishing potential alterations in the epigenetic landscape.
  
  
  METHODS
  We examined the influence of acute and chronic electroconvulsive seizure on the gene expression of histone modifiers, namely histone acetyltransferases, histone deacetylases, histone methyltransferases, and histone (lysine) demethylases as well as DNA modifying enzymes, including DNA methyltransferases, DNA demethylases, and methyl-CpG-binding proteins in the hippocampi of adult male Wistar rats using quantitative real time-PCR analysis. Further, we examined the influence of acute and chronic electroconvulsive seizure on global and residue-specific histone acetylation and methylation levels within the hippocampus, a brain region implicated in the cellular and behavioral effects of electroconvulsive seizure.
  
  
  RESULTS
  Acute and chronic electroconvulsive seizure induced a primarily unique, and in certain cases bidirectional, regulation of histone and DNA modifiers, and methyl-CpG-binding proteins, with an overlapping pattern of gene regulation restricted to Sirt4, Mll3, Jmjd3, Gadd45b, Tet2, and Tet3. Global histone acetylation and methylation levels were predominantly unchanged, with the exception of a significant decline in H3K9 acetylation in the hippocampus following chronic electroconvulsive seizure.
  
  
  CONCLUSIONS
  Electroconvulsive seizure treatment evokes the transcriptional regulation of several histone and DNA modifiers, and methyl-CpG-binding proteins within the hippocampus, with a predominantly distinct pattern of regulation induced by acute and chronic electroconvulsive seizure.
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India (Dr Pusalkar, Ms Ghosh, Ms Jaggar, Ms Husain, and Dr Vaidya); Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, Pune, Maharashtra, India (Dr Galande).
authors.forename: Madhavi
authors.initials: M
authors.lastname: Pusalkar
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India (Dr Pusalkar, Ms Ghosh, Ms Jaggar, Ms Husain, and Dr Vaidya); Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, Pune, Maharashtra, India (Dr Galande).
authors.forename: Shreya
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India (Dr Pusalkar, Ms Ghosh, Ms Jaggar, Ms Husain, and Dr Vaidya); Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, Pune, Maharashtra, India (Dr Galande).
authors.forename: Minal
authors.initials: M
authors.lastname: Jaggar
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India (Dr Pusalkar, Ms Ghosh, Ms Jaggar, Ms Husain, and Dr Vaidya); Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, Pune, Maharashtra, India (Dr Galande).
authors.forename: Basma Fatima Anwar
authors.initials: BF
authors.lastname: Husain
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India (Dr Pusalkar, Ms Ghosh, Ms Jaggar, Ms Husain, and Dr Vaidya); Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, Pune, Maharashtra, India (Dr Galande).
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Galande
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India (Dr Pusalkar, Ms Ghosh, Ms Jaggar, Ms Husain, and Dr Vaidya); Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, Pune, Maharashtra, India (Dr Galande). vvaidya@tifr.res.in.
authors.forename: Vidita A
authors.initials: VA
authors.lastname: Vaidya
keywords: DNA demethylase; DNA methyltransferase; histone acetyltransferase; histone deacetylase; histone demethylase; histone methyltransferase; methyl-CpG-binding proteins