title: Downregulation of Runx2 by 1,25-Dihydroxyvitamin D₃ Induces the Transdifferentiation of Osteoblasts to Adipocytes.
abstract: 1,25-Dihydroxyvitamin D₃ (1,25(OH)₂D₃) indirectly stimulates bone formation, but little is known about its direct effect on bone formation. In this study, we observed that 1,25(OH)₂D₃ enhances adipocyte differentiation, but inhibits osteoblast differentiation during osteogenesis. The positive role of 1,25(OH)₂D₃ in adipocyte differentiation was confirmed when murine osteoblasts were cultured in adipogenic medium. Additionally, 1,25(OH)₂D₃ enhanced the expression of adipocyte marker genes, but inhibited the expression of osteoblast marker genes in osteoblasts. The inhibition of osteoblast differentiation and promotion of adipocyte differentiation mediated by 1,25(OH)₂D₃ were compensated by Runx2 overexpression. Our results suggest that 1,25(OH)₂D₃ induces the transdifferentiation of osteoblasts to adipocytes via Runx2 downregulation in osteoblasts.
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Korea. kjhpw@hanmail.net.
authors.forename: Jung Ha
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Korea. iamsemun@gmail.com.|Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Korea. iamsemun@gmail.com.
authors.forename: Semun
authors.initials: S
authors.lastname: Seong
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Korea. kabsun@hanmail.net.
authors.forename: Kabsun
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Korea. doll517@naver.com.
authors.forename: Inyoung
authors.initials: I
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Korea. bcfrank9@gmail.com.|Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Korea. bcfrank9@gmail.com.
authors.forename: Byung-Chul
authors.initials: BC
authors.lastname: Jeong
authors.affiliation: Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Korea. nacksung@jnu.ac.kr.|Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Korea. nacksung@jnu.ac.kr.
authors.forename: Nacksung
authors.initials: N
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D054338:Cell Transdifferentiation / Q000187:drug effects*; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis* / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; D002117:Calcitriol
keywords: 1,25(OH)2D3; adipocyte; osteoblast; transdifferentiation