title: Periodic peristalsis increasing acetone-butanol-ethanol productivity during simultaneous saccharification and fermentation of steam-exploded corn straw.
abstract: The acetone-butanol-ethanol (ABE) fermentation of lignocellulose at high solids content has recently attracted extensive attention. However, the productivity of high solids ABE fermentation of lignocellulose is typically low in traditional processes due to the lack of efficient intensifying methods. In the present study, periodic peristalsis, a novel intensifying method, was applied to improve ABE production by the simultaneous saccharification and fermentation (SSF) of steam-exploded corn straw using Clostridium acetobutylicum ATCC824. The ABE concentration and the ABE productivity of SSF at a solids content of 17.5% (w/w) with periodic peristalsis were 17.1 g/L and 0.20 g/(L h), respectively, which were higher than those obtained under static conditions (15.2 g/L and 0.14 g/(L h)). The initial sugar conversion rate over the first 12 h with periodic peristalsis was 4.67 g/(L h) at 10 FPU/g cellulase dosage and 15% (w/w) solids content, an increase of 49.7% compared with the static conditions. With periodic peristalsis, the period of batch fermentation was shortened from 108 h to 84 h. The optimal operating regime was a low frequency (6 h-1) of periodic peristalsis in the acid-production phase (0-48 h) of SSF. Therefore, periodic peristalsis should be an effective intensifying method to increase the productivity of ABE fermentation at high solids content.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: hzchen@ipe.ac.cn.
authors.forename: Hongzhang
authors.initials: H
authors.lastname: Chen
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D000440:Butanols / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000378:metabolism; D046969:Clostridium acetobutylicum / Q000201:enzymology / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D009068:Movement; D013227:Steam; D013395:Sucrose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
chemical_list: D000440:Butanols; D002241:Carbohydrates; D013227:Steam; C036909:lignocellulose; D000096:Acetone; D000431:Ethanol; D013395:Sucrose; D020001:1-Butanol; D008031:Lignin; D002480:Cellulase
keywords: Acetone–butanol–ethanol fermentation; Corn straw; High solids content; Lignocellulose bioconversion; Periodic peristalsis; Simultaneous saccharification and fermentation; Steam explosion