title: Time Dependency of Chemodiversity and Biosynthetic Pathways: An LC-MS Metabolomic Study of Marine-Sourced Penicillium.
abstract: This work aimed at studying metabolome variations of marine fungal strains along their growth to highlight the importance of the parameter "time" for new natural products discovery. An untargeted time-scale metabolomic study has been performed on two different marine-derived Penicillium strains. They were cultivated for 18 days and their crude extracts were analyzed by HPLC-DAD-HRMS (High Performance Liquid Chromatography-Diode Array Detector-High Resolution Mass Spectrometry) each day. With the example of griseofulvin biosynthesis, a pathway shared by both strains, this work provides a new approach to study biosynthetic pathway regulations, which could be applied to other metabolites and more particularly new ones. Moreover, the results of this study emphasize the interest of such an approach for the discovery of new chemical entities. In particular, at every harvesting time, previously undetected features were observed in the LC-MS (Liquid Chromatography-Mass Spectrometry) data. Therefore, harvesting times for metabolite extraction should be performed at different time points to access the hidden metabolome.
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. catherine.roullier@univ-nantes.fr.|ThalassOMICS, Plateforme Corsaire, Biogenouest, Nantes 44035, France. catherine.roullier@univ-nantes.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Roullier
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. samuel.bertrand@univ-nantes.fr.|ThalassOMICS, Plateforme Corsaire, Biogenouest, Nantes 44035, France. samuel.bertrand@univ-nantes.fr.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bertrand
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. blanchet.elodie1@gmail.com.|Sorbonne Universités, UPMC Université Paris, USR 3579, LBBM, Observatoire Océanologique, Banyuls-sur-Mer 66650, France. blanchet.elodie1@gmail.com.
authors.forename: Elodie
authors.initials: E
authors.lastname: Blanchet
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. mathildepeigne@hotmail.fr.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Peigné
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. thibaut.robiou@univ-nantes.fr.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Robiou du Pont
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. yann.guitton@oniris-nantes.fr.|Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), LUNAM Université, Oniris, Nantes 44307, France. yann.guitton@oniris-nantes.fr.
authors.forename: Yann
authors.initials: Y
authors.lastname: Guitton
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. yves-francois.pouchus@univ-nantes.fr.|ThalassOMICS, Plateforme Corsaire, Biogenouest, Nantes 44035, France. yves-francois.pouchus@univ-nantes.fr.
authors.forename: Yves François
authors.initials: YF
authors.lastname: Pouchus
authors.affiliation: Faculty of Pharmacy, University of Nantes, EA 2160-Mer Molécules Santé, 9 rue Bias BP 53508, Nantes-cedex 1 44035, France. olivier.grovel@univ-nantes.fr.|ThalassOMICS, Plateforme Corsaire, Biogenouest, Nantes 44035, France. olivier.grovel@univ-nantes.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Grovel
mesh_terms: D059001:Aquatic Organisms / Q000378:metabolism*; D001688:Biological Products / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008386:Marine Biology / Q000379:methods; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics / Q000379:methods; D010407:Penicillium / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001688:Biological Products
keywords: LC-MS; biosynthesis; griseofulvin; kinetics; marine fungi; natural products; time-scale metabolomics; untargeted metabolomics