title: Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii colonization.
abstract: Real-time polymerase chain reaction (PCR)-based approaches have not been assessed in terms of their ability to detect patients colonized by Acinetobacter baumannii during active surveillance. This prospective, double-blind study demonstrated that a real-time PCR assay had high sensitivity (100%) and specificity (91.2%) compared with conventional culture for detecting A. baumannii in 397 active surveillance samples, and provided results within 3h. Receiver-operator curve analyses demonstrated that the technique has diagnostic accuracy of 97.7% (95% confidence interval 96.0-99.3%). This method could facilitate the rapid implementation of infection control measures for preventing the transmission of A. baumannii.
authors.affiliation: Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Blanco-Lobo
authors.affiliation: Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain; Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: V
authors.initials: V
authors.lastname: González-Galán
authors.affiliation: Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Quintanilla
authors.affiliation: Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Valencia
authors.affiliation: Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Cazalla
authors.affiliation: Clinical Unit of Emergency and Intensive Care Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Martín
authors.affiliation: Clinical Unit of Emergency and Intensive Care Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Alonso
authors.affiliation: Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain; Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Pérez-Romero
authors.affiliation: Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain; Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Cisneros
authors.affiliation: Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain; Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, Seville, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Aznar
authors.affiliation: Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain. Electronic address: mcconnell.mike75@gmail.com.
authors.forename: M J
authors.initials: MJ
authors.lastname: McConnell
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods; D002353:Carrier State / Q000175:diagnosis* / Q000382:microbiology; D004311:Double-Blind Method; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011446:Prospective Studies; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
keywords: Acinetobacter baumannii; Active surveillance; Colonization; Real-time PCR