title: Molecular detection and characterization of bovine viral diarrhea virus in Mongolian cattle and yaks.
abstract: Bovine viral diarrhea virus (BVDV) is classified into two species, namely, Bovine viral diarrhea virus 1 and Bovine viral diarrhea virus 2, and affects cattle worldwide, resulting in significant economic loss. The prevalence of BVDV-1 and BVDV-2 infections and its genotypes in Mongolian animals has not been studied. In this study, we surveyed BVDV infection in dairy cattle and yaks from Bornuur and Bulgan counties by RT-PCR, and the average infection rate in the sampling sites was 15.8 % and 20.0 %, respectively. In addition, molecular features of the 5'-UTR region of the BVDV genome in Mongolian cattle and yaks were identified as belonging to the subtypes BVDV-1a and BVDV-2a, respectively. Determining the prevalence, geographical distribution, and molecular diversity of BVDV-1 and BVDV-2 in various host species in Mongolia is important for further studies and process control programs.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
authors.forename: Nyamsuren
authors.initials: N
authors.lastname: Ochirkhuu
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: Laboratory of Virology, Institute of Veterinary Medicine, Mongolia University of Life Science, Khan-Uul district, Zaisan, 17042, Ulaanbaatar, Mongolia.
authors.forename: Raadan
authors.initials: R
authors.lastname: Odbileg
authors.affiliation: Laboratory of Virology, Institute of Veterinary Medicine, Mongolia University of Life Science, Khan-Uul district, Zaisan, 17042, Ulaanbaatar, Mongolia.
authors.forename: Battogtokh
authors.initials: B
authors.lastname: Odzaya
authors.affiliation: Laboratory of Virology, Institute of Veterinary Medicine, Mongolia University of Life Science, Khan-Uul district, Zaisan, 17042, Ulaanbaatar, Mongolia.
authors.forename: Shura
authors.initials: S
authors.lastname: Gansukh
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Murata
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan. okazu@vetmed.hokudai.ac.jp.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology*; D002417:Cattle / Q000821:virology; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D008297:Male; D008986:Mongolia; D010802:Phylogeny