title: Kir7.1 immunoreactivity in canine choroid plexus tumors.
abstract: Choroid plexus neoplasms are uncommon brain tumors in dogs. Choroid plexus carcinomas often spread diffusely throughout the ventricular system and subarachnoid space and, in aggressive forms, can mimic histologic patterns of other carcinomas, including being embedded in a desmoplastic reaction. Although choroid plexus tumors (CPTs) heterogeneously express pan-cytokeratin, little is known about other markers to identify choroid plexus and their associated tumors. Kir7.1, an inward-rectifier potassium channel, is reported to have high diagnostic utility in human neuropathology to distinguish CPTs from other primary brain tumors and cerebral metastases. To determine Kir7.1 expression in the dog brain, we analyzed the immunoreactivity of Kir7.1 in normal brain, gliomas, ependymomas, CPTs, meningiomas, and carcinomas. In normal brain tissue, the immunostaining was restricted to the choroid plexus where there was robust membrane immunoreactivity along the apical border of the cells with less intense cytoplasmic staining. Similar strong immunoreactivity was detected in 12 of 12 CPTs, whereas 5 of 5 gliomas, 4 of 5 ependymomas, 5 of 5 meningiomas, and 5 of 6 carcinomas had no immunoreactivity. One ependymoma and 1 nasal carcinoma with squamous metaplasia were up to 75% immunopositive, with moderate cytoplasmic and membranous immunoreactivity, but lacking the robust apical immunoreactivity pattern. Analysis for immunoreactivity in a tissue microarray failed to yield any other locations in which immunoreactivity was detected. These results, including the distinctive pattern of immunostaining in CPTs, suggest that Kir7.1 is an excellent marker for CPTs in the dog.
authors.affiliation: Section of Anatomic Pathology, Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Section of Anatomic Pathology, Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY.
authors.forename: Erica A
authors.initials: EA
authors.lastname: Sloma
authors.affiliation: Section of Anatomic Pathology, Department of Biomedical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY adm10@cornell.edu.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Miller
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D016545:Choroid Plexus Neoplasms / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D007150:Immunohistochemistry / Q000662:veterinary; D024661:Potassium Channels, Inwardly Rectifying / Q000276:immunology*
chemical_list: C489477:Kir7.1 channel; D024661:Potassium Channels, Inwardly Rectifying
keywords: Canine; Kir7.1; choroid plexus; immunohistochemistry