title: Control of Listeria monocytogenes biofilms on industrial surfaces by the bacteriocin-producing Lactobacillus sakei CRL1862.
abstract: The effect of the bacteriocin-producing Lactobacillus sakei CRL1862 and its bacteriocin in the control of Listeria biofilm formation on industrial surfaces at 10°C was investigated. A screening among different Listeria species was performed allowing selecting L. monocytogenes FBUNT for its use as a biofilm producer on stainless steel (SS) and polytetrafluoroe-thylene (PTFE) surfaces. Three conditions were simulated to evaluate the ability of the bacteriocinogenic strain to displace, exclude and compete pathogen biofilm formation. Lactobacillus sakei CRL1862 effectively inhibited biofilm formation by L. monocytogenes FBUNT through the three assayed mechanisms, pathogen inhibition being more efficient on PTFE than on SS surface. Moreover, co-culture of L. monocytogenes FBUNT with the bacteriocin-producer displayed the highest efficacy reducing the pathogen by 5.54 ± 0.12 and 4.52 ± 0.01 on PTFE and SS, respectively. Industrially, the pre-treatment with L. sakei CRL1862 or its bacteriocin (exclusion) constitutes the most realistic way to prevent pathogen biofilm settlement. The use of bacteriocins and/or the bacteriocin-producer strain represents a safe and environmentally-friendly sanitation method to mitigate post-processing food contamination.
authors.affiliation: Laboratorio de Tecnología y Desarrollo. Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, T4000ILC, Tucumán, Argentina marianapi@outlook.com.ar.
authors.forename: Mariana
authors.initials: M
authors.lastname: Pérez-Ibarreche
authors.affiliation: Laboratorio de Tecnología y Desarrollo. Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, T4000ILC, Tucumán, Argentina.
authors.forename: Patricia
authors.initials: P
authors.lastname: Castellano
authors.affiliation: Biology of Infection Unit, French National Reference Center and World Health Organization Collaborating Centre on Listeria, Institut Pasteur, 75015 Paris, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Leclercq
authors.affiliation: Laboratorio de Tecnología y Desarrollo. Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, T4000ILC, Tucumán, Argentina.
authors.forename: Graciela
authors.initials: G
authors.lastname: Vignolo
mesh_terms: D000898:Antibiosis; D001430:Bacteriocins / Q000096:biosynthesis*; D018441:Biofilms / Q000254:growth & development*; D018920:Coculture Techniques; D005506:Food Contamination / Q000517:prevention & control; D000070256:Latilactobacillus sakei / Q000502:physiology*; D008089:Listeria monocytogenes / Q000472:pathogenicity / Q000502:physiology*; D011138:Polytetrafluoroethylene; D013193:Stainless Steel; D013499:Surface Properties
chemical_list: D001430:Bacteriocins; D013193:Stainless Steel; D011138:Polytetrafluoroethylene
keywords: Lactobacillus sakei CRL1862; Listeria monocytogenes; bacteriocin; biofilm; industrial surfaces