title: First report of Metastrongylus pudendotectus by the genetic characterization of mitochondria genome of cox1 in pigs from Tibet, China.
abstract: Lungworms, a world wild distributed parasites cause serious respiratory diseases to the pigs. A high infection rate of Metastrongylus lungworms has been found in Tibetan pigs being slaughtered in different slaughter houses of Tibet autonomous region. The main aim of our study was to detect and confirm the lungworm parasite by the genetic characterization of mitochondrial cox1genome isolated from the lungs of Tibetan pig. The adult lungworms were collected from the lungs of slaughtered pigs and identification was done through morphological examinations. Total genomic DNA of the extracted worms was performed and a fragment (∼450bp) of the cox1 of mitochondrial (mt) gene was amplified. Amplicons were cloned into PGEM(®)-T Easy vector and the positive clones were sequenced from a commercial company. Sequence and phylogenetic analysis were performed by software of DNAMAN and MEGA respectively. The results revealed that the lungworms infecting the Tibetan pigs were Metastrongylus pudendotectus (M. pudendotectus). To our knowledge, this is the first report for the isolation and identification for the genetic characterization of mitochondria (mt) genome of cox1 of M. pudendotectus derived from Tibetan pigs in Tibet, China.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Houqiang
authors.initials: H
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Lan
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Han
authors.affiliation: University College of Veterinary & Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shahzad
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Laboratory of Detection and Monitoring of Highland Animal Disease, Tibet Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Qiu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Shucheng
authors.initials: S
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Wenteng
authors.initials: W
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Laboratory of Detection and Monitoring of Highland Animal Disease, Tibet Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, People's Republic of China. Electronic address: lijk210@sina.com.
authors.forename: Jiakui
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D008681:Metastrongyloidea / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D017206:Strongylida Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology*; D018609:Tibet / Q000453:epidemiology
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV
keywords: Cox-1; Genetic characterization; Lung worm; Metastrongylus pudendotectus; Tibetan pigs