title: Identification and quantification of the phosphorylated ovalbumin by high resolution mass spectrometry under dry-heating treatment.
abstract: The specific phosphorylation sites and degree of phosphorylation (DP) at each site are directly related to protein's structure and functional properties. Thus, characterizing the introduced phosphate groups is of great importance. This study was to monitor the phosphorylation sites, DP and the number of phosphorylation sites in P-Oval achieved by dry heating in the presence of pyrophosphate for 1, 2 and 5days by using Fourier transform ion cyclotron mass spectrometry (FTICR MS). Two phosphorylation sites were found in natural ovalbumin, but the number of phosphorylation sites increased to 8, 8 and 10 after dry-heating phosphorylation for 1, 2 and 5days, respectively. In addition, dual-phosphorylated peptides were detected for samples without extensive heating. The phosphorylation sites were found to be mainly on Ser residues, which could be the preferred phosphorylation site for dry heating in the presence of pyrophosphate.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China. Electronic address: tuzc_mail@aliyun.com.
authors.forename: Zong-Cai
authors.initials: ZC
authors.lastname: Tu
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
authors.forename: Guang-Xian
authors.initials: GX
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D005583:Fourier Analysis; D006361:Heating; D013058:Mass Spectrometry / Q000379:methods; D010047:Ovalbumin / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology
chemical_list: D010446:Peptide Fragments; D010047:Ovalbumin
keywords: Dry-heating treatment; Mass spectrometry; Ovalbumin; Pepsin; Phosphorylation