title: MiRNA expression profile of ionizing radiation-induced liver injury in mouse using deep sequencing.
abstract: In order to investigate the potential regulatory roles of microRNAs (miRNAs) in mouse response to ionizing radiation (IR), the small RNA libraries from liver tissues of mice with or without ionizing radiation (IR) were sequenced by high-throughput deep sequencing technology. A total of 270 miRNAs including 212 known and 58 potentially novel miRNAs were identified. Within these miRNAs, there were 48 miRNAs that were differentially expressed, including 27 known and 21 novel miRNAs. The results of quantitative RT-polymerase chain reaction (qRT-PCR) were in consistent with the sequencing analysis. Target gene prediction, function annotation, and pathway of the identified miRNAs were analyzed using RNAhybrid, miRanda software and Swiss-Prot, Gene Ontology (GO), Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes, and Genomes (KEGG) and non-redundant (NR) databases. These results should be useful to investigate the biological function of miRNAs under IR-induced liver injury.
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.|Department of People's Liberation Army, The Quartermaster Equipment Institute of General Logistics, Beijing, 100010, China.
authors.forename: Jike
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of People's Liberation Army, The Quartermaster Equipment Institute of General Logistics, Beijing, 100010, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of People's Liberation Army, The Quartermaster Equipment Institute of General Logistics, Beijing, 100010, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, China.
authors.forename: Naixun
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Food Science and Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, 150090, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D020869:Gene Expression Profiling; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D008099:Liver / Q000293:injuries / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D011833:Radiation Injuries, Experimental / Q000235:genetics* / Q000378:metabolism; D011839:Radiation, Ionizing
chemical_list: D035683:MicroRNAs
keywords: deep sequencing; irradiation; liver; miRNA; mouse