title: New insight on obesity and adipose-derived stem cells using comprehensive metabolomics.
abstract: Obesity affects the functional capability of adipose-derived stem cells (ASCs) and their effective use in regenerative medicine through mechanisms that are still poorly understood. In the present study we used a multiplatform [LC/MS, GC/MS and capillary electrophoresis/MS (CE/MS)], metabolomics, untargeted approach to investigate the metabolic alteration underlying the inequalities observed in obesity-derived ASCs. The metabolic fingerprint (metabolites within the cells) and footprint (metabolites secreted in the culture medium), from obesity- and non-obesity-derived ASCs of humans or mice, were characterized to provide valuable information. Metabolites associated with glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway and the polyol pathway were increased in the footprint of obesity-derived human ASCs, indicating alterations in carbohydrate metabolism, whereas, from the murine model, deep differences in lipid and amino acid catabolism were highlighted. Therefore, new insights on the ASCs' metabolome were provided that enhance our understanding of the processes underlying ASCs' stemness capacity and its relationship with obesity, in different cell models.
authors.affiliation: Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain.
authors.forename: Annalaura
authors.initials: A
authors.lastname: Mastrangelo
authors.affiliation: Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain.
authors.forename: María I
authors.initials: MI
authors.lastname: Panadero
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Pérez
authors.affiliation: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain School of Doctoral Studies & Research, Universidad Europea (UE), Madrid, Spain.
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: Gálvez
authors.affiliation: Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: García
authors.affiliation: Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain.
authors.forename: Coral
authors.initials: C
authors.lastname: Barbas
authors.affiliation: Centre for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668, Madrid, Spain ruperez@ceu.es.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Rupérez
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D002952:Citric Acid Cycle / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D006019:Glycolysis / Q000502:physiology; D006801:Humans; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D009765:Obesity / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
keywords: adipose-derived stem cells; endo-metabolome; exo-metabolome; metabolomics; obesity