title: C1q-binding anti-HLA antibody assay: A test dilemma.
abstract: Detection of donor-specific anti-HLA antibodies in patients with kidney graft, or awaiting kidney graft, acts as a predictor for antibody mediated rejection. Several methods are in practice for the detection of anti-HLA antibodies; including the latest introduction of C1q-binding anti-HLA antibody method. This method depends on detecting complement fixing anti-HLA antibodies on single antigen beads using C1q as the marker for the presence of those antibodies. Here we discuss recent data on this method and present a working hypothesis for explaining the inability of this method to detect low titer anti-HLA antibodies.
authors.affiliation: Department of Pathology and Laboratory Medicine, College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, National Guard Health Affairs, Riyadh, Saudi Arabia.
authors.forename: Ali H
authors.initials: AH
authors.lastname: Hajeer
mesh_terms: D000906:Antibodies / Q000097:blood* / Q000276:immunology*; D000911:Antibodies, Monoclonal; D015922:Complement C1q / Q000378:metabolism*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards*
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D015922:Complement C1q