title: Role of alkaline phosphatase intestine-isomerase in acute mesenteric ischemia diagnosis.
abstract: BACKGROUND
  The aim of the present study was to investigate the diagnostic value of alkaline phosphatase (ALP) intestine-isomerase, plasma lactate dehydrogenase (LDH), and D-dimer levels in acute mesenteric ischemia.
  
  
  METHODS
  Thirty Wistar rats were divided into 5 groups of 6 rats each. In Group 1, blood samples were obtained to determine normal parameter levels. In the sham group, Group 2, blood samples were obtained following laparotomy. In Group 3, blood samples were obtained 2 hours after ligation. In Groups 4 and 5, blood samples were obtained at 4 and 6 hours after ligation, respectively. Ischemic damage was assessed using a pathological scoring system. Blood samples were analyzed for hourly changes in parameters.
  
  
  RESULTS
  No statistically significant difference in D-dimer levels was found between ischemia groups (p=0.337). A statistically significant difference in LDH levels was found between the control group, Group 1, and Group 4 (p=0.018). ALP intestine-isomerase enzyme levels were not statistically significant in other groups (p=0.077).
  
  
  CONCLUSIONS
  Findings indicate that plasma LDH levels higher than 1900 IU/L may be a useful marker in the early diagnosis of acute mesenteric obstruction. However, ALP intestine-isomerase enzyme and D-dimer plasma levels were not found to contribute to the diagnosis.
authors.affiliation: Department of General Surgery, Gülhane Military Medical Academy, Ankara-Turkey. lapsekiliemin@yahoo.com.
authors.forename: Emin
authors.initials: E
authors.lastname: Lapsekili
authors.affiliation: Department of General Surgery, Gülhane Military Medical Academy, Ankara-Turkey.
authors.forename: Öner
authors.initials: Ö
authors.lastname: Menteş
authors.affiliation: Department of General Surgery, Gülhane Military Medical Academy, Ankara-Turkey.
authors.forename: Müjdat
authors.initials: M
authors.lastname: Balkan
authors.affiliation: Department of Pathology, Gülhane Military Medical Academy, Ankara-Turkey.
authors.forename: Armağan
authors.initials: A
authors.lastname: Günal
authors.affiliation: Department of Biochemistry, Gülhane Military Medical Academy, Ankara-Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Yaman
authors.affiliation: Department of General Surgery, Gülhane Military Medical Academy, Ankara-Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Kozak
authors.affiliation: Department of General Surgery, Gülhane Military Medical Academy, Ankara-Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Peker
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D007527:Isoenzymes / Q000097:blood; D007535:Isomerases / Q000097:blood; D065666:Mesenteric Ischemia / Q000097:blood / Q000175:diagnosis*; D012372:ROC Curve; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D005338:Fibrin Fibrinogen Degradation Products; D007527:Isoenzymes; C036309:fibrin fragment D; C415670:Alpi protein, rat; D000469:Alkaline Phosphatase; D007535:Isomerases