title: Glutaredoxin 3 promotes nasopharyngeal carcinoma growth and metastasis via EGFR/Akt pathway and independent of ROS.
abstract: Glutaredoxin 3 (GLRX3) is antioxidant enzyme, maintaining a low level of ROS, thus contributing to the survival and metastasis of several types of cancer. However, the expression and functions of GLRX3 have not been addressed in nasopharyngeal carcinoma (NPC). In this study, we found that GLRX3 was overexpressed in NPC. Knockdown of GLRX3 in NPC cell lines inhibited proliferation in vitro, tumorignesis in vivo, and colony formation. In addition, GLRX3 knockdown decreased the migration and invasion capacity of NPC cells by reversing the epithelial-mesenchymal transition (EMT). Furthermore, stabilization of GLRX3 was positively related to with epidermal growth factor receptor (EGFR) expression and negatively with ROS generation. Phosphorylation of Akt, a key downstream effector, was induced by EGFR signaling but did not rely on increasing ROS level in NPC cells. GLRX3 might be an oncoprotein in NPC, playing important roles in increasing redox reaction and activating EGFR/ Akt signals, so it may be a therapeutic target for NPC.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: You
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Shixing
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine, Mie, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Murata
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Guangwu
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D002352:Carrier Proteins / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D017382:Reactive Oxygen Species; D015398:Signal Transduction / Q000502:physiology
chemical_list: D002352:Carrier Proteins; C404105:GLRX3 protein, human; D017382:Reactive Oxygen Species; C512478:EGFR protein, human; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; EGFR; glutaredoxin 3; nasopharyngeal carcinoma