title: Circulating Cell-free miRNA Expression and its Association with Clinicopathologic Features in Inflammatory and Non- Inflammatory Breast Cancer.
abstract: Recent discovery showing the presence of microRNAs (miRNAs) in the circulation sparked interest in their use as potential biomarkers. Our previous studies showed the diagnostic potential of miR-451 as a serological marker for inflammatory breast cancer (IBC), miR-337- 5p and miR-30b for non-inflammatory breast cancer (non-IBC). The aim of this study is to investigate the prognostic values of circulating miRNAs by comparing the amounts of 12 circulating miRNAs in the serum of IBC and non-IBC from Tunisian breast cancer patients, and by determinating whether correlated pairs of miRNAs could provide useful information in the diagnosis of IBC and non-IBC patients. TaqMan qPCR was performed to detect circulating expression of miRNAs in serum of 20 IBC, 20 non-IBC and 20 healthy controls. Nonparametric rank Spearman rho correlation coefficient was used to examine the prognostic value of miRNAs and to assess the correlation profile between miRNAs expression. Further, a large number of miRNAs were highly correlated (rho>0.5) in both patients groups and controls. Also, the correlations profiles were different between IBC, non-IBC and healthy controls indicating important changes in molecular pathways in cancer cells. Our results showed that miR-335 was significantly overexpressed in premenopausal non-IBC patients; miR-24 was significantly overexpressed in non-IBC postmenopausal patients. Patients with previous parity had higher serum of miR-342-5p levels than those without. Furthermore, patients with HER2+ IBC present lower serum levels of miR-15a than patients with HER2- disease. Together, these results underline the potential of miRNAs to function as diagnostic and prognostic markers for IBC and non-IBC, with links to the menopausal state, Her2 status and parity.
authors.affiliation: Laboratory of Genetics, Faculty of Sciences, University of Tunis El Manar, Tunis, Tunisia E-mail : hamdi.khouloud@yahoo.fr.
authors.forename: K
authors.initials: K
authors.lastname: Hamdi
authors.forename: J
authors.initials: J
authors.lastname: Blancato
authors.forename: D
authors.initials: D
authors.lastname: Goerlitz
authors.forename: Md
authors.initials: M
authors.lastname: Islam
authors.forename: B
authors.initials: B
authors.lastname: Neili
authors.forename: A
authors.initials: A
authors.lastname: Abidi
authors.forename: A
authors.initials: A
authors.lastname: Gat
authors.forename: F Ben
authors.initials: FB
authors.lastname: Ayed
authors.forename: S
authors.initials: S
authors.lastname: Chivi
authors.forename: Ca
authors.initials: C
authors.lastname: Loffredo
authors.forename: I
authors.initials: I
authors.lastname: Jillson
authors.forename: A Benammar
authors.initials: AB
authors.lastname: Elgaaied
authors.forename: R
authors.initials: R
authors.lastname: Marrakchi
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D058922:Inflammatory Breast Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs