title: Clinical evaluation of salivary carbohydrate antigen 125 and leptin in controls and parotid tumours.
abstract: OBJECTIVE
  We determined the correlation between saliva and serum for CA125 and leptin, and evaluated their clinical screening potential for parotid tumours.
  
  
  METHODS
  Serum, acid-stimulated bilateral parotid saliva and chewing-stimulated whole saliva were collected and measured the levels of CA125 and leptin with electrochemiluminescence immunoassay and enzyme-linked immunosorbent assay for healthy controls and patients with unilateral parotid tumour. Intra- and intergroup comparisons were made among them. Correlations and receiver operating curve analyses were also conducted.
  
  
  RESULTS
  There was no correlation between salivary and serum CA125 (r = -0.157-0.265, P > 0.05), while significant correlation was found for leptin (r = 0.219-0.761, P < 0.05). Leptin levels in tumour parotid saliva and CA125 levels in whole saliva were elevated significantly (P < 0.001) and showed screening potential for parotid tumours. Salivary and serum leptin levels were significantly higher in women than in men (P < 0.001).
  
  
  CONCLUSIONS
  Salivary CA125 might originate primarily from salivary gland and tumour rather than from blood, while salivary leptin might originate from both blood and salivary gland. Multiple sources might contribute to the significantly elevated CA125 in whole saliva. Whole saliva CA125 and parotid saliva leptin reflected the occurrence of parotid tumours, while serum CA125 and leptin did not. Salivary CA125 and leptin could not distinguish malignant parotid tumours. When detecting leptin level, the influence of subjects' sex must be considered.
authors.affiliation: Salivary Gland Disease Center of Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China.|Inpatient Department of Oral and Maxillofacial Surgery, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China.
authors.forename: K-Y
authors.initials: KY
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Liu
authors.affiliation: School of Biomedical Engineering, Capital Medical University, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Hua
authors.affiliation: Salivary Gland Disease Center of Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China.|Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China.
authors.forename: S-L
authors.initials: SL
authors.lastname: Wang
authors.affiliation: Salivary Gland Disease Center of Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China. lijun3021@aliyun.com.|Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China. lijun3021@aliyun.com.
authors.forename: J
authors.initials: J
authors.lastname: Li
mesh_terms: D018394:CA-125 Antigen / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D020738:Leptin / Q000032:analysis* / Q000097:blood; D008163:Luminescent Measurements; D008297:Male; D008565:Membrane Proteins / Q000032:analysis* / Q000097:blood; D010307:Parotid Neoplasms / Q000097:blood / Q000737:chemistry* / Q000175:diagnosis; D012463:Saliva / Q000737:chemistry*
chemical_list: D018394:CA-125 Antigen; D020738:Leptin; C451759:MUC16 protein, human; D008565:Membrane Proteins
keywords: carbohydrate antigen 125; leptin; parotid tumour; saliva; serum