title: A new method for long-term storage of titred microbial standard solutions suitable for microbiologic quality control activities of pharmaceutical companies.
abstract: Commercially available lyophilized microbial standards are expensive and subject to reduction in cell viability due to freeze-drying stress. Here we introduce an inexpensive and straightforward method for in-house microbial standard preparation and cryoconservation that preserves constant cell titre and cell viability over 14 months.
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, centro di ricerca per l'Agrobiologia e la Pedologia CREA-ABP, Piazza M. D'Azeglio 30, 50121 Firenze, Italy.
authors.forename: Carolina
authors.initials: C
authors.lastname: Chiellini
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, centro di ricerca per l'Agrobiologia e la Pedologia CREA-ABP, Piazza M. D'Azeglio 30, 50121 Firenze, Italy. Electronic address: stefano.mocali@crea.gov.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mocali
authors.affiliation: Laboratory of Microbial and Molecular Evolution, Dept of Biology, University of Florence, Via Madonna del Piano 6, I-50019 Sesto F.no Florence, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Fani
authors.affiliation: Istituto De Angeli S.R.L. Pharma Division Località Prulli, n°103/C-50066-Reggello, FI, Italy.
authors.forename: Iolanda
authors.initials: I
authors.lastname: Ferro
authors.affiliation: ANGELINI S.p.A. Quality Control Via Vecchia del Pinocchio, 22-60131 Ancona, Italy.
authors.forename: Serenella
authors.initials: S
authors.lastname: Bruschi
authors.affiliation: Istituto De Angeli S.R.L. Pharma Division Località Prulli, n°103/C-50066-Reggello, FI, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pinzani
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D008828:Microbiological Techniques / Q000592:standards*; D011786:Quality Control; D012015:Reference Standards; D013678:Technology, Pharmaceutical / Q000379:methods*
keywords: Cryopreservation; Pharmacopoeia; Standard reference strains