title: Reproducible pharmacogenomic profiling of cancer cell line panels.
abstract: The use of large-scale genomic and drug response screening of cancer cell lines depends crucially on the reproducibility of results. Here we consider two previously published screens, plus a later critique of these studies. Using independent data, we show that consistency is achievable, and provide a systematic description of the best laboratory and analysis practices for future studies.
authors.affiliation: Department of Bioinformatics and Computational Biology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Haverty
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Eva
authors.initials: E
authors.lastname: Lin
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Jenille
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Billy
authors.initials: B
authors.lastname: Lam
authors.affiliation: Department of Bioinformatics and Computational Biology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Lianoglou
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Neve
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Martin
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Jeff
authors.initials: J
authors.lastname: Settleman
authors.affiliation: Department of Discovery Oncology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Yauch
authors.affiliation: Department of Bioinformatics and Computational Biology, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Bourgon
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods* / Q000592:standards*; D005819:Genetic Markers / Q000235:genetics; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D005819:Genetic Markers