title: Superheated water extraction of glycyrrhizic acid from licorice root.
abstract: Superheated water extraction (SWE) has become an interesting green extraction method for different classes of compounds. In this study, SWE was used to extract glycyrrhizic acid (GA) from licorice root. Response surface methodology (RSM) was applied to evaluate and optimize the extraction conditions. The influence of operating conditions such as water temperature (100, 120 and 140°C) and solvent flow rates (1, 3 and 5mL/min) were investigated at 0.5mm mean particle size and 20bar pressure. Separation and identification of the glycyrrhizic acid, as the main component, was carried out by the RP-HPLC method. The best operating conditions for the SWE of licorice were determined to be 100°C temperature,15mL/min flow rate and 120min extraction time. The results showed that the amount of the obtained GA was relatively higher using SWE (54.760mg/g) than the Soxhlet method (28.760mg/g) and ultrasonic extraction (18.240mg/g).
authors.affiliation: Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Shabkhiz
authors.affiliation: Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran. Electronic address: eikani@irost.ir.
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Eikani
authors.affiliation: Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran.
authors.forename: Zeinolabedin
authors.initials: Z
authors.lastname: Bashiri Sadr
authors.affiliation: Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Golmohammad
mesh_terms: D000975:Antioxidants; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006035:Glycyrrhiza / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000737:chemistry*; D006358:Hot Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D014867:Water; D019695:Glycyrrhizic Acid
keywords: Glycyrrhizic acid; Licorice extract; RSM; Superheated water extraction