title: Local Immune Response in Helicobacter pylori Infection.
abstract: There have been few studies concerning the cytokine profiles in gastric mucosa of Helicobacter pylori-infected patients with normal mucosa, chronic gastritis, and gastric carcinoma (GAC).In the present study, we aimed to elucidate the genomic expression levels and immune pathological roles of cytokines-interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-4, IL-6, IL-10, transforming growth factor (TGF)-β, IL-17A, IL-32-in H pylori-infected patients with normal gastric mucosa (NGM; control), chronic active gastritis (CAG), and GAC. Genomic expression levels of these cytokines were assayed by real-time PCR analysis in gastric biopsy specimens obtained from 93 patients.We found that the genomic expression levels of IFN-γ, TNF-α, IL-6, IL-10, IL-17A mRNA were increased in the CAG group and those of TNF-α, IL-6, IL-10, IL-17A, TGF-β mRNA were increased in the GAC group with reference to H pylori-infected NGM group.This study is on the interest of cytokine profiles in gastric mucosa among individuals with normal, gastritis, or GAC. Our findings suggest that the immune response of gastric mucosa to infection of H pylori differs from patient to patient. For individual therapy, levels of genomic expression of IL-6 or other cytokines may be tracked in patients.
authors.affiliation: From the Department of Medical Oncology (DKS), Faculty of Medicine, Akdeniz University, Antalya; Faculty of Health Sciences (MS), Kahramanmaras Sutcu Imam University, Kahramanmaras; Department of Microbiology (SK); and Department of Gastroenterology (HA, IS), Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Kivrak Salim
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sahin
authors.forename: Sadi
authors.initials: S
authors.lastname: Köksoy
authors.forename: Haydar
authors.initials: H
authors.lastname: Adanir
authors.forename: Inci
authors.initials: I
authors.lastname: Süleymanlar
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000276:immunology*; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry* / Q000276:immunology; D005756:Gastritis / Q000276:immunology*; D015870:Gene Expression; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D055815:Young Adult
chemical_list: D007378:Interleukins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma