title: Bergamottin isolated from Citrus bergamia exerts in vitro and in vivo antitumor activity in lung adenocarcinoma through the induction of apoptosis, cell cycle arrest, mitochondrial membrane potential loss and inhibition of cell migration and invasion.
abstract: The objective of the present study was to investigate the in vitro and in vivo anticancer properties of bergamottin, a natural furanocoumarin, against human non-small cell lung carcinoma (NSCLC) A549 cells. We also studied its effect on cell proliferation, cell cycle arrest, cell invasion, cell migration as well as cell apoptosis. Antiproliferative activity of bergamottin was estimated by the MTT assay. Phase contrast and fluorescence microscopy as well as flow cytometry using Annexin V-FITC assay were used to study induction of apoptosis by bergamottin in these cells. The effects of bergamottin on cell cycle phase distribution as well as on mitochondrial membrane potential were also demonstrated using flow cytometry. In vitro wound healing assay was used to study the effect of bergamottin on cell migration. The effects of bergamottin on tumor progression were also observed using a nude mouse model. The mice were divided into 4 groups and treated with bergamottin injected intraperitoneally. Bergamottin induced dose-dependent as well as time-dependent cytotoxic effects as well as inhibition of colony formation in the A549 cancer cells. Bergamottin also suppressed cancer cell invasion as well as cancer cell migration. Phase contrast microscopy and fluorescence microscopy revealed that bergamottin induced cell shrinkage, chromatin condensation and the cells became rounded and detached from each other. Bergamottin also induced a potent cell cycle arrest at the G2/M phase of the cell cycle. Experiments in mice showed that 25, 50 and 100 mg/kg bergamottin injection reduced the tumor weight from 1.61 g in the phosphate-buffered saline (PBS)-treated group (control) to 1.21, 0.42 and 0.15 g in the bergamottin-treated groups, respectively. The results of the present study revealed that bergamottin was able to inhibit lung cancer cell growth both in a cell model and a xenograft mouse model by inducing apoptosis, mitochondrial membrane potential loss, G2/M cell cycle arrest as well as inhibiting cell migration and invasion.
authors.affiliation: Department of Internal Medicine, The Affiliated Tumor Hospital of Zhengzhou University/Tumor Hospital of Henan Province, Zhengzhou, Henan 450008, P.R. China.
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Wu
authors.affiliation: Department of Internal Medicine, The Affiliated Tumor Hospital of Zhengzhou University/Tumor Hospital of Henan Province, Zhengzhou, Henan 450008, P.R. China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Wu
authors.affiliation: Department of Internal Medicine, The Affiliated Tumor Hospital of Zhengzhou University/Tumor Hospital of Henan Province, Zhengzhou, Henan 450008, P.R. China.
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Zhao
authors.affiliation: Department of Internal Medicine, The Affiliated Tumor Hospital of Zhengzhou University/Tumor Hospital of Henan Province, Zhengzhou, Henan 450008, P.R. China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Department of Internal Medicine, The Affiliated Tumor Hospital of Zhengzhou University/Tumor Hospital of Henan Province, Zhengzhou, Henan 450008, P.R. China.
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Zou
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002957:Citrus / Q000737:chemistry; D005260:Female; D011564:Furocoumarins / Q000494:pharmacology*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D011564:Furocoumarins; C068337:bergamottin