title: Vascular control of the Drosophila haematopoietic microenvironment by Slit/Robo signalling.
abstract: Self-renewal and differentiation of mammalian haematopoietic stem cells (HSCs) are controlled by a specialized microenvironment called 'the niche'. In the bone marrow, HSCs receive signals from both the endosteal and vascular niches. The posterior signalling centre (PSC) of the larval Drosophila haematopoietic organ, the lymph gland, regulates blood cell differentiation under normal conditions and also plays a key role in controlling haematopoiesis under immune challenge. Here we report that the Drosophila vascular system also contributes to the lymph gland homoeostasis. Vascular cells produce Slit that activates Robo receptors in the PSC. Robo activation controls proliferation and clustering of PSC cells by regulating Myc, and small GTPase and DE-cadherin activity, respectively. These findings reveal that signals from the vascular system contribute to regulating the rate of blood cell differentiation via the regulation of PSC morphology.
authors.affiliation: Centre de Biologie du Développement, UMR 5547 CNRS/Université Toulouse III and Fédération de Recherche de Biologie de Toulouse, 118 route de Narbonne 31062 Toulouse cedex 9, France.
authors.forename: Ismaël
authors.initials: I
authors.lastname: Morin-Poulard
authors.affiliation: Centre de Biologie du Développement, UMR 5547 CNRS/Université Toulouse III and Fédération de Recherche de Biologie de Toulouse, 118 route de Narbonne 31062 Toulouse cedex 9, France.
authors.forename: Anurag
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Centre de Biologie du Développement, UMR 5547 CNRS/Université Toulouse III and Fédération de Recherche de Biologie de Toulouse, 118 route de Narbonne 31062 Toulouse cedex 9, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Louradour
authors.affiliation: Centre de Biologie du Développement, UMR 5547 CNRS/Université Toulouse III and Fédération de Recherche de Biologie de Toulouse, 118 route de Narbonne 31062 Toulouse cedex 9, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vanzo
authors.affiliation: Centre de Biologie du Développement, UMR 5547 CNRS/Université Toulouse III and Fédération de Recherche de Biologie de Toulouse, 118 route de Narbonne 31062 Toulouse cedex 9, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Vincent
authors.affiliation: Centre de Biologie du Développement, UMR 5547 CNRS/Université Toulouse III and Fédération de Recherche de Biologie de Toulouse, 118 route de Narbonne 31062 Toulouse cedex 9, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Crozatier
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002319:Cardiovascular System / Q000378:metabolism; D004330:Drosophila / Q000166:cytology / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism*; D006410:Hematopoiesis; D007814:Larva / Q000166:cytology / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D000097905:Roundabout Proteins
chemical_list: D015820:Cadherins; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D011509:Proteoglycans; D011971:Receptors, Immunologic; D020764:cdc42 GTP-Binding Protein; D000097905:Roundabout Proteins; C420997:dlp protein, Drosophila; C501889:shg protein, Drosophila; C066819:sli protein, Drosophila