title: A new NOE-mediated MT signal at around -1.6ppm for detecting ischemic stroke in rat brain.
abstract: In the present work, we reported a new nuclear Overhauser enhancement (NOE)-mediated magnetization transfer (MT) signal at around -1.6ppm (NOE(-1.6)) in rat brain and investigated its application in the detection of acute ischemic stroke in rodent model. Using continuous wave (CW) MT sequence, the NOE(-1.6) is reliably detected in rat brain. The amplitude of this new NOE signal in rat brain was quantified using a 5-pool Lorentzian Z-spectral fitting method. Amplitudes of amide, amine, NOE at -3.5ppm (NOE(-3.5)), as well as NOE(-1.6) were mapped using this fitting method in rat brain. Several other conventional imaging parameters (R1, R2, apparent diffusion coefficient (ADC), and semi-solid pool size ratio (PSR)) were also measured. Our results show that NOE(-1.6), R1, R2, ADC, and APT signals from stroke lesion have significant changes at 0.5-1h after stroke. Compared with several other imaging parameters, NOE(-1.6) shows the strongest contrast differences between stroke and contralateral normal tissues and stays consistent over time until 2h after onset of stroke. Our results demonstrate that this new NOE(-1.6) signal in rat brain is a new potential contrast for assessment of acute stroke in vivo and might provide broad applications in the detection of other abnormal tissues.
authors.affiliation: Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, USA.
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neurological Surgery, Vanderbilt University, Nashville, TN, USA.
authors.forename: Aqeela
authors.initials: A
authors.lastname: Afzal
authors.affiliation: Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, USA; Deparment of Physics and Astronomy, Vanderbilt University, Nashville, TN, USA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
authors.forename: Junzhong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, USA; Deparment of Physics and Astronomy, Vanderbilt University, Nashville, TN, USA; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Gore
authors.affiliation: Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, USA; Deparment of Physics and Astronomy, Vanderbilt University, Nashville, TN, USA.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Gochberg
authors.affiliation: Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA; Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, USA. Electronic address: zhongliang.zu@vanderbilt.edu.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Zu
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000473:pathology; D004195:Disease Models, Animal; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051381:Rats; D012815:Signal Processing, Computer-Assisted; D020521:Stroke / Q000000981:diagnostic imaging* / Q000473:pathology
keywords: Chemical exchange saturation transfer (CEST); Ischemic stroke; Magnetization transfer (MT); Nuclear Overhauser enhancement (NOE)