title: Mechanism of baicalin compatibility in chinese medicine formula Banxia Xiexin Decoction () by pharmacokinetics and indirect competitive enzyme-linked immunosorbent assays in mice.
abstract: OBJECTIVE
  To determine the effects of different formulations of Banxia Xiexin Decoction ( , BXD) on the pharmacokinetics of baicalin (BAL) in mice.
  
  
  METHODS
  Pungent, bitter, and sweet components of BXD (totaling 7 Chinese herbs) were formulated into the following groups: K (bitter herbs), XK (pungent and bitter herbs), KG (bitter and sweet herbs), and BXD (all 7 herbs) groups. These different formulations were administered intragastrically in mice, and blood was collected via the tail vein for continuous monitoring. BAL, which is a main active constituent in Scutellaria baicalensis Georgi., was detected in this study. Indirect competitive enzyme-linked immunosorbent assays (icELISAs) based on anti-BAL-monoclonal antibodies were employed to determine BAL concentrations in each group.
  
  
  RESULTS
  The concentrations of BAL in blood samples from mice in the K and XK groups were lower than those in other groups. In all groups, BAL concentrations peaked at around 1-1.5 h and again at 5-7 h. There were no significant differences in the timing of peak BAL concentrations between groups. However, the peak concentrations and area under curve (AUC)0-36 hin the KG and BXD groups were almost 3 times of those in the K and XK groups.
  
  
  CONCLUSIONS
  Differing compatibilities of BXD caused dissimilar pharmacokinetics of BAL. Moreover, we demonstrated a method for the continuous detection of blood concentrations of Chinese medicines in mice, and icELISA may be a feasible technique for the study of pharmcokinetic mechanisms of Chinese medicine.
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Qu
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Qu
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Liu
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Qin
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China. zhaoyandr@gmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Wang
keywords: Banxia Xiexin Decoction; Chinese medicine; baicalin; indirect competitive enzyme-linked immunosorbent assay; pharmacokinetics; prescription compatibility