title: Extraction and Quantification of Tryptophan and Kynurenine from Cultured Cells and Media Using a High Performance Liquid Chromatography (HPLC) System Equipped with an Ultra-Sensitive Diode Array Detector.
abstract: Evidence of the involvement of tryptophan and its metabolite, kynurenine, in various biological processes including cancer is constantly expanding. Analysis of cell extracts and culture media can allow for quick snapshots of the metabolic fluctuations occurringin vitro. Here, we describe a method for metabolite extraction from mammalian cells and analysis of extracted metabolites and cell culture media by HPLC with detection using an ultra-sensitive diode array detector.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, University of California, Davis, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Kim
authors.affiliation: Lawrence Livermore National Laboratory, Livermore, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Stewart
authors.affiliation: Division of Nephrology, Department of Internal Medicine, University of California, Davis, USA; Cancer Center, University of California, Davis, USA; Medical Service, Sacramento VA Medical Center, Sacramento, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Weiss