title: The Unicellular Ancestry of Groucho-Mediated Repression and the Origins of Metazoan Transcription Factors.
abstract: Groucho is a co-repressor that interacts with many transcription factors playing a crucial role in animal development. The evolutionary origins of Groucho are not clear. It is generally regarded as being a distinct animal-specific protein, although with similarities to the yeast Tup-like proteins. Here, it is shown that Groucho has true orthologs in unicellular relatives of animals. Based on their phylogenetic distribution, and an analysis of ligand-binding residues, these genes are unlikely to be orthologs of the fungal Tup-like genes. By identifying conserved candidate Groucho interaction motifs (GIMs) in nonmetazoan transcription factors, it is demonstrated that the details of molecular interactions between Groucho and transcription factors are likely to have been established prior to the origin of animals, but that the association of GIMs with many transcription factor types can be regarded as a metazoan innovation.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), 181 chemin du Lazaret, 06230 Villefranche-sur-mer, France copley@obs-vlfr.fr.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Copley
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D019295:Computational Biology; D008024:Ligands; D009687:Nuclear Proteins / Q000235:genetics*; D010802:Phylogeny; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D000071678:WD40 Repeats / Q000235:genetics
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D008024:Ligands; D009687:Nuclear Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C066502:TUP1 protein, S cerevisiae; C091023:gro protein, Drosophila
keywords: animal evolution; transcription factor evolution; transcriptional repression