title: [Effect of DAPK overexpression on biological behaviors and caspase-3 expression in HL-60 cells].
abstract: OBJECTIVE
  To explore the effect of DAPK overexpression on the biological behaviors and caspase-3 expression in HL-60 cells.
  
  
  METHODS
  The expression of DAPK mRNA was detected by RT-PCR leukemia cell lines K562, Molt4, U937, and HL-60 cells. HL-60 cells were transfected by a eukaryotic expression vector pReceiver-M29-DAPK via LipofectamineTM 2000, and the impact of DAPK overexpression on cell apoptosis, cell cycle, cell differentiation and caspase-3 expression were analyzed.
  
  
  RESULTS
  DAPK mRNA expression was positive in K562, Molt4 and U937 cells but negative in HL-60 cells. Significantly increased cell apoptosis was observed in pReceiver-M29-DAPK-transfected HL-60 cells by flow cytometry and Hoechst33342 staining. The cell cycle distribution and differentiation showed no significant changes after the transfection. The expression of caspase-3 was significantly increased in the cells after transfection.
  
  
  CONCLUSIONS
  DAPK gene overexpression promotes apoptosis of HL-60 cells without affecting the cell cycle and differentiation. Caspase-3 may be involved in the regulation of cell apoptosis.
authors.affiliation: Department of Hematology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.E-mail: zhaowh21@163.com.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhao
authors.forename: Fan-Yi
authors.initials: FY
authors.lastname: Meng
authors.forename: Yong-Rong
authors.initials: YR
authors.lastname: Lai
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Peng
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D002453:Cell Cycle; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D064550:Death-Associated Protein Kinases / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D020298:U937 Cells
chemical_list: D012333:RNA, Messenger; C579096:DAPK1 protein, human; D064550:Death-Associated Protein Kinases; C505407:CASP3 protein, human; D053148:Caspase 3