title: CRISPR-Mediated Base Editing without DNA Double-Strand Breaks.
abstract: Targeting point mutations using CRISPR/Cas9 so far has required efficient homologous recombination (HR) and donor oligonucleotides. In a recent Nature paper, Komor and colleagues (2016) describe a way to make specific base changes that does not depend on HR or donor DNA and does not involve making double-strand breaks.
authors.affiliation: Molecular Cell, 50 Hampshire Street, Cambridge, MA 02139, USA. Electronic address: bplosky@cell.com.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Plosky
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D053903:DNA Breaks, Double-Stranded; D000072669:Gene Editing / Q000379:methods*; D059765:Homologous Recombination; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D017354:Point Mutation