title: The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases.
abstract: Since the creation of the first transgenic rabbit thirty years ago, pronuclear microinjection remained the single applied method and resulted in numerous important rabbit models of human diseases, including cardiac deficiencies, albeit with low efficiency. For additive transgenesis a novel transposon mediated method, e.g., the Sleeping Beauty transgenesis, increased the efficiency, and its application to create cardiac disease models is expected in the near future. The targeted genome engineering nuclease family, e.g., the zink finger nuclease (ZFN), the transcription activator-like effector nuclease (TALEN) and the newest, clustered regularly interspaced short palindromic repeats (CRISPR) with the CRISPR associated effector protein (CAS), revolutionized the non-mouse transgenesis. The latest gene-targeting technology, the CRISPR/CAS system, was proven to be efficient in rabbit to create multi-gene knockout models. In the future, the number of tailor-made rabbit models produced with one of the above mentioned methods is expected to exponentially increase and to provide adequate models of heart diseases.
authors.affiliation: Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, H-2100 Gödöllő, Hungary. Electronic address: bosze@abc.hu.
authors.forename: Z
authors.initials: Z
authors.lastname: Bősze
authors.affiliation: Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, H-2100 Gödöllő, Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Major
authors.affiliation: Department of Pharmacology & Pharmacotherapy, University of Szeged, H-6720 Szeged, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Baczkó
authors.affiliation: Department of Cardiology and Angiology I, University Heart Center Freiburg, D-79106 Freiburg, Germany.
authors.forename: K E
authors.initials: KE
authors.lastname: Odening
authors.affiliation: Department of Pharmacology & Pharmacotherapy, University of Szeged, H-6720 Szeged, Hungary; Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, H-2100 Gödöllő, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Bodrogi
authors.affiliation: Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, H-2100 Gödöllő, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Hiripi
authors.affiliation: Department of Pharmacology & Pharmacotherapy, University of Szeged, H-6720 Szeged, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Varró
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements / Q000235:genetics; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D023281:Genomics; D006331:Heart Diseases; D006801:Humans; D011817:Rabbits
chemical_list: D004251:DNA Transposable Elements
keywords: Designer nucleases; Knock-in; Knockout; Sleeping beauty transposon; Transgenic rabbit