title: Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction.
abstract: Intradermal adipose tissue plays an essential role for hair follicles (HFs) regeneration by regulating hair cycles. However, the effect of reconstruction of HFs and the involvement of adipose-related cells are poorly understood. We investigated assembly strategies for the interactions of dermal papilla (DP) cells with adipose-derived stem cells (ASCs) in promoting hair formation. DP cells lose DP traits during adherent culture, but preserved DP markers with a unified sphere diameter by seeding on chitosan-coated microenvironments. Next, ASCs isolated from rats were co-cultured with DP spheres by different assembling approaches to determine their interactions; a mixed sphere of ASCs with DP cells (MA-DPS), or a core-shell structure, outer ASCs shell and an inner DP core (CSA-DPS). CSA-DPS exhibited superior DP characteristics compared to MA-DPS. Conditional medium from ASCs, but not differentiated adipocytes, promoted DP markers and functional alkaline phosphatase activity from the DP cells. In vivo patch assay showed the core-shell assembling of CSA-DPS can reconstruct cellular arrangements and microenvironmental niches as dominated by PPARα signal in ASCs to induce the greater hair induction than MA-DPS or DP spheres alone. Therefore, the assembling of a core-shell sphere for DP with ASCs could reconstruct the HF cellular arrangement for hair formation. This paper set the groundwork for further evaluation of the input of other cell types.
authors.affiliation: Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Chin-Fu
authors.initials: CF
authors.lastname: Huang
authors.affiliation: Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Ya-Ju
authors.initials: YJ
authors.lastname: Chang
authors.affiliation: Division of Plastic Surgery, National Cheng Kung University Hospital, Tainan, 701, Taiwan.
authors.forename: Yuan-Yu
authors.initials: YY
authors.lastname: Hsueh
authors.affiliation: Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Huang
authors.affiliation: Division of Plastic Surgery, National Cheng Kung University Hospital, Tainan, 701, Taiwan.
authors.forename: Duo-Hsiang
authors.initials: DH
authors.lastname: Wang
authors.affiliation: Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Tzu-Chieh
authors.initials: TC
authors.lastname: Huang
authors.affiliation: Division of Plastic Surgery, National Cheng Kung University Hospital, Tainan, 701, Taiwan.|Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Wu
authors.affiliation: Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Fong-Chin
authors.initials: FC
authors.lastname: Su
authors.affiliation: International Research Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, 701, Taiwan.|Institute of Clinical Medicine, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Michael
authors.initials: M
authors.lastname: Hughes
authors.affiliation: International Research Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, 701, Taiwan.|Institute of Clinical Medicine, National Cheng Kung University, Tainan, 701, Taiwan.|Department of Pathology, University of Southern California, California 90033, USA.
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Chuong
authors.affiliation: Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan.|Institute of Basic Medical Science, National Cheng Kung University, Tainan, 701, Taiwan.|Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan, 701, Taiwan.|International Research Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, 701, Taiwan.
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Wu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000737:chemistry; D020405:Dermis / Q000166:cytology* / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D047493:PPAR alpha / Q000378:metabolism; D051381:Rats; D018874:Spheroids, Cellular / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D047493:PPAR alpha; D048271:Chitosan; D000469:Alkaline Phosphatase