title: In Vitro Antioxidant, Anti-Diabetes, Anti-Dementia, and Inflammation Inhibitory Effect of Trametes pubescens Fruiting Body Extracts.
abstract: Trametes pubescens, white rot fungus, has been used for folk medicine in Asian countries to treat ailments such as cancer and gastrointestinal diseases. This study was initiated to evaluate the in vitro antioxidant, anti-diabetes, anti-dementia, and anti-inflammatory activities of T. pubescens fruiting bodies. The 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activities of T. pubescens methanol (ME) and hot water (HWE) extracts (2.0 mg/mL) were comparable to butylated hydroxytoluene (BHT), the positive control. However, the chelating effects of ME and HWE were significantly higher than that of BHT. The HWE (6 mg/mL) also showed comparable reducing power to BHT. Eleven phenol compounds were detected by high performance liquid chromatography (HPLC) analysis. The α-amylase and α-glucosidase inhibitory activities of the ME and HWE of the mushroom were lower than Acarbose, the standard reference; however, the inhibitory effects of the mushroom extracts at 2.0 mg/mL were moderate. The acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory effects of ME and HWE were moderate and comparable with galanthamine, the standard drug to treat early stages of Alzheimer's disease (AD). The ME had a neuroprotective effect against glutamate-induced PC-12 cell cytotoxicity at the concentration range of 2-40 μg/mL. The mushroom extracts also showed inflammation inhibitory activities such as production of nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-induced murine macrophage-like cell lines (RAW 264.7) and significantly suppressed the carrageenan-induced rat paw-edema. Therefore, fruiting body extracts of T. pubescens demonstrated antioxidant related anti-diabetes, anti-dementia and anti-inflammatory activities.
authors.affiliation: Division of Life Sciences, Incheon National University, (Songdo-dong) 119 Academy-ro, Yeonsu-gu, Incheon 406-772, Korea. khim61@inu.ac.kr.
authors.forename: Kyung Hoan
authors.initials: KH
authors.lastname: Im
authors.affiliation: Division of Life Sciences, Incheon National University, (Songdo-dong) 119 Academy-ro, Yeonsu-gu, Incheon 406-772, Korea. tknguyen@inu.ac.kr.
authors.forename: Trung Kien
authors.initials: TK
authors.lastname: Nguyen
authors.affiliation: Division of Life Sciences, Incheon National University, (Songdo-dong) 119 Academy-ro, Yeonsu-gu, Incheon 406-772, Korea. jaehyukc@inu.ac.kr.
authors.forename: Jaehyuk
authors.initials: J
authors.lastname: Choi
authors.affiliation: Division of Life Sciences, Incheon National University, (Songdo-dong) 119 Academy-ro, Yeonsu-gu, Incheon 406-772, Korea. tslee@incheon.ac.kr.
authors.forename: Tae Soo
authors.initials: TS
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D002351:Carrageenan / Q000633:toxicity; D003704:Dementia / Q000188:drug therapy; D003920:Diabetes Mellitus / Q000188:drug therapy; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D016166:Free Radical Scavengers / Q000737:chemistry; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy; D010636:Phenols / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D055454:Trametes / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols; D002351:Carrageenan
keywords: Trametes pubescens; anti-dementia; anti-diabetes; anti-inflammation; antioxidant