title: Scrape Loading/Dye Transfer Assay.
abstract: The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.
authors.affiliation: RECETOX-Research Centre for Toxic Compounds in the Environment, Masaryk University, Kamenice 753/5, Brno, 62500, Czech Republic. babica@recetox.muni.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Babica
authors.affiliation: RECETOX-Research Centre for Toxic Compounds in the Environment, Masaryk University, Kamenice 753/5, Brno, 62500, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Sovadinová
authors.affiliation: Department of Pediatrics and Human Development, and the Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA.
authors.forename: Brad L
authors.initials: BL
authors.lastname: Upham
mesh_terms: D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D003911:Dextrans / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D017629:Gap Junctions / Q000378:metabolism* / Q000648:ultrastructure; D007546:Isoquinolines / Q000378:metabolism; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D012235:Rhodamines / Q000378:metabolism; D012708:Sertoli Cells
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; D007546:Isoquinolines; D012235:Rhodamines; C051311:rhodamine dextran; C017475:lucifer yellow
keywords: Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers