title: Polarized trafficking of the sorting receptor SorLA in neurons and MDCK cells.
abstract: The sorting receptor SorLA is highly expressed in neurons and is also found in other polarized cells. The receptor has been reported to participate in the trafficking of several ligands, some of which are linked to human diseases, including the amyloid precursor protein, TrkB, and Lipoprotein Lipase (LpL). Despite this, only the trafficking in nonpolarized cells has been described so far. Due to the many differences between polarized and nonpolarized cells, we examined the localization and trafficking of SorLA in epithelial Madin-Darby canine kidney (MDCK) cells and rat hippocampal neurons. We show that SorLA is mainly found in sorting endosomes and on the basolateral surface of MDCK cells and in the somatodendritic domain of neurons. This polarized distribution of SorLA respectively depends on an acidic cluster and an extended version of this cluster and involves the cellular adaptor complex AP-1. Furthermore, we show that SorLA can mediate transcytosis across a tight cell layer.
authors.affiliation: Department of Biomedicine, The Lundbeck Foundation Initiative on Brain Barriers and Drug Delivery, Aarhus University, Denmark.|Department of Biomedicine, The MIND Centre, Aarhus University, Denmark.|Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Stine C
authors.initials: SC
authors.lastname: Klinger
authors.affiliation: Department of Biomedicine, The Lundbeck Foundation Initiative on Brain Barriers and Drug Delivery, Aarhus University, Denmark.|Department of Biomedicine, The MIND Centre, Aarhus University, Denmark.
authors.forename: Anne
authors.initials: A
authors.lastname: Højland
authors.affiliation: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Shweta
authors.initials: S
authors.lastname: Jain
authors.affiliation: Department of Biomedicine, The MIND Centre, Aarhus University, Denmark.|Department of Biomedicine, The Danish Diabetes Academy, Aarhus University, Denmark.
authors.forename: Mads
authors.initials: M
authors.lastname: Kjolby
authors.affiliation: Department of Biomedicine, The Lundbeck Foundation Initiative on Brain Barriers and Drug Delivery, Aarhus University, Denmark.|Department of Biomedicine, The MIND Centre, Aarhus University, Denmark.
authors.forename: Peder
authors.initials: P
authors.lastname: Madsen
authors.affiliation: Department of Biomedicine, The Lundbeck Foundation Initiative on Brain Barriers and Drug Delivery, Aarhus University, Denmark.|Department of Biomedicine, The MIND Centre, Aarhus University, Denmark.
authors.forename: Anna Dorst
authors.initials: AD
authors.lastname: Svendsen
authors.affiliation: Department of Medical Biosciences, Physiological Chemistry, Umeå University, Sweden.
authors.forename: Gunilla
authors.initials: G
authors.lastname: Olivecrona
authors.affiliation: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Juan S
authors.initials: JS
authors.lastname: Bonifacino
authors.affiliation: Department of Biomedicine, The Lundbeck Foundation Initiative on Brain Barriers and Drug Delivery, Aarhus University, Denmark.|Department of Biomedicine, The MIND Centre, Aarhus University, Denmark.
authors.forename: Morten S
authors.initials: MS
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology*; D004285:Dogs; D011992:Endosomes / Q000378:metabolism; D057809:HEK293 Cells; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000737:chemistry / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D057900:Transcytosis / Q000235:genetics / Q000502:physiology
chemical_list: D026502:LDL-Receptor Related Proteins
keywords: AP1; LR11; SorLA; adaptor protein; cell polarity; epithelial cell; intracellular trafficking; neurons; sorting