title: Pathological and ultrastructural observations and liver function analysis of Eimeria stiedai-infected rabbits.
abstract: To study the pathogenicity of Eimeria stiedai, sporulated oocysts were given orally to coccidian-free two-month-old New Zealand rabbits(1000±20g). After 30days, blood samples from the rabbit hearts were collected for routine blood tests, liver functions and four characteristics of blood coagulation. Additionally, specimens of the liver, bile duct and duodenum were collected to observe the changes in pathology and ultrastructure. E. stiedai severely restricted the growth and development of rabbits. Blood tests showed that glutamine transferase (GGT) and serum cholinesterase (ChE) were significantly different from the non-infected controls. Other extremely significant differences were observed in the biochemical indices of routine blood tests, liver function and four blood coagulation characteristics, indicating that the liver functions were significantly affected. Staining showed that, compared with the negative control group, the liver, bile duct and duodenum contained significant numbers of lesions, and organs and cell structures suffered severe damage in ultrastructure, which greatly affecting bodily functions. E. stiedai-infected rabbits model was successfully established, which might provide a theoretical basis for research on the pathogenesis of rabbit coccidia, and the diagnosis and prevention of coccidiosis in rabbits.
authors.affiliation: Laboratory Animal Center of Nantong University, No. 19 Qixiu Road, Chongchuan District, Nantong, Jiangsu 226001, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Jing
authors.affiliation: Laboratory Animal Center of Nantong University, No. 19 Qixiu Road, Chongchuan District, Nantong, Jiangsu 226001, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Laboratory Animal Center of Nantong University, No. 19 Qixiu Road, Chongchuan District, Nantong, Jiangsu 226001, PR China.
authors.forename: Shun-Xing
authors.initials: SX
authors.lastname: Zhu
authors.affiliation: Laboratory Animal Center of Nantong University, No. 19 Qixiu Road, Chongchuan District, Nantong, Jiangsu 226001, PR China.
authors.forename: Ying-Mei
authors.initials: YM
authors.lastname: Jiang
authors.affiliation: Laboratory Animal Center of Nantong University, No. 19 Qixiu Road, Chongchuan District, Nantong, Jiangsu 226001, PR China.
authors.forename: Liu-Cheng
authors.initials: LC
authors.lastname: Wu
authors.affiliation: Laboratory Animal Center of Nantong University, No. 19 Qixiu Road, Chongchuan District, Nantong, Jiangsu 226001, PR China. Electronic address: songhongyanv@163.com.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Song
authors.affiliation: Laboratory Animal Center of Nantong University, No. 19 Qixiu Road, Chongchuan District, Nantong, Jiangsu 226001, PR China. Electronic address: shaoyx1956@163.com.
authors.forename: Yi-Xiang
authors.initials: YX
authors.lastname: Shao
mesh_terms: D000818:Animals; D003048:Coccidiosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D004539:Eimeria / Q000145:classification*; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008111:Liver Function Tests / Q000662:veterinary*; D011817:Rabbits
keywords: Eimeria stiedai; Liver; Liver function; Pathology; Ultrastructure