title: Generation and Purification of Tetraploid Cells.
abstract: Tetraploid cells are genetically unstable and have the capacity to promote the development and/or progression of human malignancies. It is now estimated that ~40 % of all solid tumors have passed through a tetraploid intermediate stage at some point during their development. Understanding the biological characteristics of tetraploid cells that impart oncogenic properties is therefore a highly relevant and fundamentally important aspect of cancer biology. Here, we describe strategies to efficiently generate and purify tetraploid cells for use in cell biological studies.
authors.affiliation: Departments of Pharmacology & Experimental Therapeutics and Medicine, Division of Hematology and Oncology, Boston University School of Medicine, Boston, MA, 02118, USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Shenk
authors.affiliation: Departments of Pharmacology & Experimental Therapeutics and Medicine, Division of Hematology and Oncology, Boston University School of Medicine, Boston, MA, 02118, USA. nganem@bu.edu.
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Ganem
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D002469:Cell Separation; D048749:Cytokinesis; D005434:Flow Cytometry; D006801:Humans; D008938:Mitosis; D057891:Tetraploidy
keywords: Centrosome; Cytokinesis; FUCCI; Hippo; Mitotic slippage; Polyploid