title: Identification of a New Genotype of African Swine Fever Virus in Domestic Pigs from Ethiopia.
abstract: African swine fever (ASF) is an important emerging transboundary animal disease (TAD), which currently has an impact on many countries in Africa, Eastern Europe, the Caucasus and the Russian Federation. The current situation in Europe shows the ability of the virus to rapidly spread, which stands to threaten the global swine industry. At present, there is no viable vaccine to minimize spread of the disease and stamping out is the main source of control. In February 2011, Ethiopia had reported its first suspected outbreaks of ASF. Genomic analyses of the collected ASF virus (ASFV) strains were undertaken using 23 tissue samples collected from domestic swine in Ethiopia from 2011 to 2014. The analysis of Ethiopian ASFVs partial p72 gene sequence showed the identification of a new genotype, genotype XXIII, that shares a common ancestor with genotypes IX and X, which comprise isolates circulating in Eastern African countries and the Republic of Congo. Analysis of the p54 gene also followed the p72 pattern and the deduced amino acid sequence of the central variable region (CVR) of the B602L gene showed novel tetramer repeats not previously characterized.
authors.affiliation: Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
authors.forename: J E
authors.initials: JE
authors.lastname: Achenbach
authors.affiliation: European Union Reference Laboratory for ASF: Centro de Investigación en Sanidad Animal, INIA, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Gallardo
authors.affiliation: OIE Reference Laboratory for ASF: VISAVET Health Surveillance Centre, Universidad Complutense Madrid, Madrid, Spain.|Animal Health Department, Universidad Complutense Madrid, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Nieto-Pelegrín
authors.affiliation: OIE Reference Laboratory for ASF: VISAVET Health Surveillance Centre, Universidad Complutense Madrid, Madrid, Spain.|Animal Health Department, Universidad Complutense Madrid, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Rivera-Arroyo
authors.affiliation: National Veterinary Institute, Debre Ziet, Ethiopia.
authors.forename: T
authors.initials: T
authors.lastname: Degefa-Negi
authors.affiliation: European Union Reference Laboratory for ASF: Centro de Investigación en Sanidad Animal, INIA, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Arias
authors.affiliation: National Veterinary Institute, Debre Ziet, Ethiopia.
authors.forename: S
authors.initials: S
authors.lastname: Jenberie
authors.affiliation: National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia.
authors.forename: D D
authors.initials: DD
authors.lastname: Mulisa
authors.affiliation: National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia.
authors.forename: D
authors.initials: D
authors.lastname: Gizaw
authors.affiliation: Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.|National Veterinary Institute, Debre Ziet, Ethiopia.
authors.forename: E
authors.initials: E
authors.lastname: Gelaye
authors.affiliation: Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.|National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia.
authors.forename: T R
authors.initials: TR
authors.lastname: Chibssa
authors.affiliation: National Veterinary Institute, Debre Ziet, Ethiopia.
authors.forename: A
authors.initials: A
authors.lastname: Belaye
authors.affiliation: Institute for Veterinary Disease Control, Austrian Agency for Health and Food Safety, Mödling, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Loitsch
authors.affiliation: National Animal Health Diagnostic and Investigation Center (NAHDIC), Sebeta, Ethiopia.
authors.forename: M
authors.initials: M
authors.lastname: Forsa
authors.affiliation: National Veterinary Institute, Debre Ziet, Ethiopia.
authors.forename: M
authors.initials: M
authors.lastname: Yami
authors.affiliation: Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Diallo
authors.affiliation: European Union Reference Laboratory for ASF: Centro de Investigación en Sanidad Animal, INIA, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Soler
authors.affiliation: Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
authors.forename: C E
authors.initials: CE
authors.lastname: Lamien
authors.affiliation: OIE Reference Laboratory for ASF: VISAVET Health Surveillance Centre, Universidad Complutense Madrid, Madrid, Spain.|Animal Health Department, Universidad Complutense Madrid, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
mesh_terms: D000357:African Swine Fever / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D000358:African Swine Fever Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D004196:Disease Outbreaks / Q000662:veterinary; D005002:Ethiopia / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine
keywords: 
  CVR
  ; African swine fever; Ethiopia; genotype; p72