title: Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells.
abstract: Betulinic acid (BA) is a pentacyclic triterpene that can be isolated from many medicinal plants around the world. The aim of this study was to evaluate the genotoxic potential of BA and its effect on the genotoxicity induced by different mutagens in V79 cells using the cytokinesis-block micronucleus assay. Different BA concentrations were combined with methyl methanesulfonate (MMS), doxorubicin (DXR), camptothecin (CPT), and etoposide (VP-16). The frequencies of micronuclei in cultures treated with different BA concentrations did not differ from those of the negative control. Treatment with BA and MMS resulted in lower micronucleus frequencies than those observed for cultures treated with MMS alone. On the other hand, a significant increase in micronucleus frequencies was observed in cultures treated with BA combined with DXR or VP-16 when compared to these mutagens alone. The results showed no effect of BA on CPT-induced genotoxicity. Therefore, BA was not genotoxic under the present experimental conditions and exerted a different influence on the genotoxicity induced by different mutagens. The modulatory effect of BA depends on the type of mutagen and concentrations used.
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Nathália Oliveira
authors.initials: NO
authors.lastname: Acésio
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Pollyanna Francielli
authors.initials: PF
authors.lastname: de Oliveira
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Daiane Fernanda Pereira
authors.initials: DF
authors.lastname: Mastrocola
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Ildercílio Mota de Souza
authors.initials: IM
authors.lastname: Lima
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Carla Carolina
authors.initials: CC
authors.lastname: Munari
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Vânia Luiza Ferreira Lucatti
authors.initials: VL
authors.lastname: Sato
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Andressa Aparecida Silva
authors.initials: AA
authors.lastname: Souza
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Lúzio Gabriel Bocalon
authors.initials: LG
authors.lastname: Flauzino
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Wilson Roberto
authors.initials: WR
authors.lastname: Cunha
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Denise Crispim
authors.initials: DC
authors.lastname: Tavares