title: The nitrogen responsive transcriptome in potato (Solanum tuberosum L.) reveals significant gene regulatory motifs.
abstract: Nitrogen (N) is the most important nutrient for the growth of potato (Solanum tuberosum L.). Foliar gene expression in potato plants with and without N supplementation at 180 kg N ha(-1) was compared at mid-season. Genes with consistent differences in foliar expression due to N supplementation over three cultivars and two developmental time points were examined. In total, thirty genes were found to be over-expressed and nine genes were found to be under-expressed with supplemented N. Functional relationships between over-expressed genes were found. The main metabolic pathway represented among differentially expressed genes was amino acid metabolism. The 1000 bp upstream flanking regions of the differentially expressed genes were analysed and nine overrepresented motifs were found using three motif discovery algorithms (Seeder, Weeder and MEME). These results point to coordinated gene regulation at the transcriptional level controlling steady state potato responses to N sufficiency.
authors.affiliation: Department of Plant Science, McGill University, 21111 Lakeshore Rd, Ste-Anne-de-Bellevue, QC, H9W5B8 Canada.
authors.forename: José Héctor
authors.initials: JH
authors.lastname: Gálvez
authors.affiliation: Agriculture and Agri-Food Canada, Fredericton Research and Development Centre, PO Box 20280, 850 Lincoln Rd., Fredericton, NB, E3B 4Z7 Canada.
authors.forename: Helen H
authors.initials: HH
authors.lastname: Tai
authors.affiliation: Agriculture and Agri-Food Canada, Fredericton Research and Development Centre, PO Box 20280, 850 Lincoln Rd., Fredericton, NB, E3B 4Z7 Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Lagüe
authors.affiliation: Agriculture and Agri-Food Canada, Fredericton Research and Development Centre, PO Box 20280, 850 Lincoln Rd., Fredericton, NB, E3B 4Z7 Canada.
authors.forename: Bernie J
authors.initials: BJ
authors.lastname: Zebarth
authors.affiliation: Department of Plant Science, McGill University, 21111 Lakeshore Rd, Ste-Anne-de-Bellevue, QC, H9W5B8 Canada.
authors.forename: Martina V
authors.initials: MV
authors.lastname: Strömvik
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D000596:Amino Acids; D009584:Nitrogen