title: Induction of Poly(ADP-ribose) Polymerase in Mouse Bone Marrow Stromal Cells Exposed to 900 MHz Radiofrequency Fields: Preliminary Observations.
abstract: Background. Several investigators have reported increased levels of poly(ADP-ribose) polymerase-1 (PARP-1), a nuclear enzyme which plays an important role in the repair of damaged DNA, in cells exposed to extremely low dose ionizing radiation which does not cause measurable DNA damage. Objective. To examine whether exposure of the cells to nonionizing radiofrequency fields (RF) is capable of increasing messenger RNA of PARP-1 and its protein levels in mouse bone marrow stromal cells (BMSCs). Methods. BMSCs were exposed to 900 MHz RF at 120 μW/cm(2) power intensity for 3 hours/day for 5 days. PARP-1 mRNA and its protein levels were examined at 0, 0.5, 1, 2, 4, 6, 8, and 10 hours after exposure using RT-PCR and Western blot analyses. Sham-exposed (SH) cells and those exposed to ionizing radiation were used as unexposed and positive control cells. Results. BMSCs exposed to RF showed significantly increased expression of PARP-1 mRNA and its protein levels after exposure to RF while such changes were not observed in SH-exposed cells. Conclusion. Nonionizing RF exposure is capable of inducing PARP-1.
authors.affiliation: School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Qina
authors.initials: Q
authors.lastname: He
authors.affiliation: School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zong
authors.affiliation: School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tong
authors.affiliation: School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D004790:Enzyme Induction; D059630:Mesenchymal Stem Cells / Q000201:enzymology* / Q000528:radiation effects*; D051379:Mice; D011065:Poly(ADP-ribose) Polymerases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011846:Radio Waves
chemical_list: D012333:RNA, Messenger; D011065:Poly(ADP-ribose) Polymerases