title: The effects of MCA-MAO on cAMP pathway in rats with cerebral hemorrhage.
abstract: To explore the effects of MCA-MAO on the cAMP pathway in rats with cerebral hemorrhage.
  
  
  
  Forty SD male rats were randomly divided into four groups: the sham operation group (n=10), the model group (n=10), the negative control group (n=10) and the experimental group (n=10). To prepare rat models for cerebral hemorrhage, autogenous femoral arterial blood was injected into the caudate nucleus. In the case of rats in sham operation group, normal saline was injected into the caudate nucleus. Rats in the negative control group received a proper amount of saline via an injection into the abdominal cavity. Rats in the experimental group were injected with 500 μL/kg MCA-MAO into the abdominal cavity. Five rats from each group were executed after 1 to 3 days, the water contents of gray and white matters were detected using far infrared moisture analyzer, the MAO activity was measured by the histochemical method. The cAMP level was measured by radio-immunity method and the protein kinase A (PKA) level was measured by Western blot. cAMP response element binding (CREB) mRNA expression level was detected by RT-PCR.
  
  
  
  Water content, MAO activity, cAMP, PKA, and CREB mRNA expression levels in the model, and the negative control groups were significantly higher than those of the sham operation and the experimental groups, the differences were statistically significant (p<0.05).
  
  
  
  MAO may mediate the pathophysiological process of hemorrhage via cAMP signaling pathway.
authors.affiliation: Department of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. xia_jianhua1@163.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: X-F
authors.initials: XF
authors.lastname: Wang
authors.forename: Y-L
authors.initials: YL
authors.lastname: Niu
authors.forename: J-H
authors.initials: JH
authors.lastname: Xia
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives; D000818:Animals; D002543:Cerebral Hemorrhage; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D008297:Male; D008995:Monoamine Oxidase; D051381:Rats
chemical_list: D015124:8-Bromo Cyclic Adenosine Monophosphate; C044258:8-chloro-cyclic adenosine monophosphate; D008995:Monoamine Oxidase; D017868:Cyclic AMP-Dependent Protein Kinases