title: High-performance Liquid Chromatographic Ultraviolet Detection of Nilotinib in Human Plasma from Patients with Chronic Myelogenous Leukemia, and Comparison with Liquid Chromatography-Tandem Mass Spectrometry.
abstract: BACKGROUND
  A method for determining nilotinib concentration in human plasma is proposed using high-performance liquid chromatography and ultraviolet detection.
  
  
  METHODS
  Nilotinib and the internal standard dasatinib were separated using a mobile phase of 0.5% Na2PO4H2O (pH 2.5)-acetonitrile-methanol (55:25:20, v/v/v) on a Capcell Pak C18 MG II column (250 × 4.6 mm) at a flow rate of 1.0 ml/min, and ultraviolet measurement at 250 nm.
  
  
  RESULTS
  The calibration curve exhibited linearity over the nilotinib concentration range of 50-2,500 ng/ml at 250 nm, with relative standard deviations (n = 5) of 7.1%, 2.5%, and 2.9% for 250, 1,500, and 2,500 ng/ml, respectively. The detection limit for nilotinib was 5 ng/ml due to three blank determinations (ρ = 3).
  
  
  CONCLUSIONS
  This method was successfully applied to assaying nilotinib in human plasma samples from patients with chronic myelogenous leukemia. In addition, we compared the results with those measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) at BML, Inc. (a commercial laboratory). A strong correlation was observed between the nilotinib concentrations measured by our high-performance liquid chromatographic method and those obtained by LC/MS-MS (r2= 0.988, P < 0.01).
authors.affiliation: Department of Clinical Pharmacy, Oita University Hospital, Yufu City, Japan. ryosuke-n@oita-u.ac.jp.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakahara
authors.affiliation: Department of Clinical Pharmacy, Oita University Hospital, Yufu City, Japan.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Satho
authors.affiliation: Department of Clinical Pharmacy, Oita University Hospital, Yufu City, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Itoh
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood*; D057230:Limit of Detection; D011743:Pyrimidines / Q000032:analysis / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011743:Pyrimidines; C498826:nilotinib
keywords: assay; high-performance liquid chromatography; liquid chromatography tandem mass spectrometry; nilotinib