title: Crystal Structure of Hypusine-Containing Translation Factor eIF5A Bound to a Rotated Eukaryotic Ribosome.
abstract: Eukaryotic translation initiation factor eIF5A promotes protein synthesis by resolving polyproline-induced ribosomal stalling. Here, we report a 3.25-Å resolution crystal structure of eIF5A bound to the yeast 80S ribosome. The structure reveals a previously unseen conformation of an eIF5A-ribosome complex and highlights a possible functional link between conformational changes of the ribosome during protein synthesis and the eIF5A-ribosome association.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, 1 rue Laurent Fries, 67404 Illkirch-Graffenstaden, France.|Université de Strasbourg, 4 Rue Blaise Pascal, 67081 Strasbourg, France.
authors.forename: Sergey
authors.initials: S
authors.lastname: Melnikov
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, 1 rue Laurent Fries, 67404 Illkirch-Graffenstaden, France.|Université de Strasbourg, 4 Rue Blaise Pascal, 67081 Strasbourg, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Mailliot
authors.affiliation: Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Byung-Sik
authors.initials: BS
authors.lastname: Shin
authors.affiliation: Institute of Organic Chemistry, Leopold Franzens University, Innrain 80/82, A-6020, Innsbruck, Austria.
authors.forename: Lukas
authors.initials: L
authors.lastname: Rigger
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, 1 rue Laurent Fries, 67404 Illkirch-Graffenstaden, France.|Université de Strasbourg, 4 Rue Blaise Pascal, 67081 Strasbourg, France.
authors.forename: Gulnara
authors.initials: G
authors.lastname: Yusupova
authors.affiliation: Institute of Organic Chemistry, Leopold Franzens University, Innrain 80/82, A-6020, Innsbruck, Austria.
authors.forename: Ronald
authors.initials: R
authors.lastname: Micura
authors.affiliation: Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Dever
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964, 1 rue Laurent Fries, 67404 Illkirch-Graffenstaden, France.|Université de Strasbourg, 4 Rue Blaise Pascal, 67081 Strasbourg, France.
authors.forename: Marat
authors.initials: M
authors.lastname: Yusupov
mesh_terms: D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010448:Peptide Initiation Factors / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A
keywords: crystallography; eIF5A; hypusine; ribosome; structure