title: An in vivo invertebrate evaluation system for identifying substances that suppress sucrose-induced postprandial hyperglycemia.
abstract: Sucrose is a major sweetener added to various foods and beverages. Excessive intake of sucrose leads to increases in blood glucose levels, which can result in the development and exacerbation of lifestyle-related diseases such as obesity and diabetes. In this study, we established an in vivo evaluation system using silkworms to explore substances that suppress the increase in blood glucose levels caused by dietary intake of sucrose. Silkworm hemolymph glucose levels rapidly increased after intake of a sucrose-containing diet. Addition of acarbose or voglibose, α-glycosidase inhibitors clinically used for diabetic patients, suppressed the dietary sucrose-induced increase in the silkworm hemolymph glucose levels. Screening performed using the sucrose-induced postprandial hyperglycemic silkworm model allowed us to identify some lactic acid bacteria that inhibit the increase in silkworm hemolymph glucose levels caused by dietary intake of sucrose. The inhibitory effects of the Lactococcus lactis #Ll-1 bacterial strain were significantly greater than those of different strains of lactic acid bacteria. No effect of the Lactococcus lactis #Ll-1 strain was observed in silkworms fed a glucose diet. These results suggest that the sucrose diet-induced postprandial hyperglycemic silkworm is a useful model for evaluating chemicals and lactic acid bacteria that suppress increases in blood glucose levels.
authors.affiliation: Teikyo University Institute of Medical Micology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.|Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 111-0033, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 111-0033, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishii
authors.affiliation: Teikyo University Institute of Medical Micology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.|Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 111-0033, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sekimizu
mesh_terms: D020909:Acarbose / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D012831:Bombyx / Q000378:metabolism* / Q000382:microbiology; D019422:Dietary Sucrose / Q000009:adverse effects*; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors / Q000008:administration & dosage* / Q000494:pharmacology; D006458:Hemolymph / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D006943:Hyperglycemia / Q000139:chemically induced* / Q000188:drug therapy*; D007294:Inositol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D056546:Lactobacillales / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D010802:Phylogeny; D019518:Postprandial Period
chemical_list: D019422:Dietary Sucrose; D065089:Glycoside Hydrolase Inhibitors; D007294:Inositol; D005947:Glucose; C102817:voglibose; D020909:Acarbose