title: In Vitro Intestinal Absorption and Metabolism of Magnoflorine and its Potential Interaction in Coptidis Rhizoma Decoction in Rat.
abstract: OBJECTIVE
  In our previous studies, it was found that there existed pharmacokinetic interactions between magnoflorine and the rest of the ingredients in Coptidis Rhizoma. In this study, the pharmacokinetic interaction mechanism of magnoflorine with the rest of the components in Coptidis Rhizoma was researched based on the intestinal absorption and metabolism characteristics.
  
  
  METHODS
  The absorption characteristics of magnoflorine in each rat intestinal segments were evaluated by non-everted intestinal sac model. To identify the metabolites of magnoflorine, the acceptor solutions of each intestinal segment at 120 min were analyzed by HPLC-LTQ-Orbitrap MS.
  
  
  RESULTS
  The accumulative absorption (Q), the absorption rate (J) and the apparent permeability coefficient (Papp) of magnoflorine were increased in duodenum, jejunum, ileum and colon of the Coptidis Rhizoma group as compared to the magnoflorine group, but there was no statistical difference between the two groups (P > 0.05). Four phase I metabolites of magnoflorine were identified in intestinal acceptor solutions of pure compound, while eight metabolites were detected in that of Coptidis Rhizoma decoction including six phase I metabolites and two phase II metabolic products.
  
  
  CONCLUSIONS
  It was shown that the rest of the ingredients in Coptidis Rhizoma accelerated the absorption of magnoflorine weakly and promoted the metabolism of magnoflorine in the gut. The effects of other processes in the pharmacokinetics should be further evaluated.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Baojuan
authors.initials: B
authors.lastname: Xue
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.|Jiamusi University, Jiamusi, 154007, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Su
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Miao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China. zhyj227@126.com.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Institutes for Food and Drug Control, No.2, Tiantan Xili, Beijing, 100050, China. masc@nifdc.org.cn.
authors.forename: Shuangcheng
authors.initials: S
authors.lastname: Ma
mesh_terms: D000818:Animals; D001060:Aporphines / Q000302:isolation & purification / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000089042:Coptis chinensis; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001060:Aporphines; D004365:Drugs, Chinese Herbal; C107393:Coptidis rhizoma extract; C001670:magnoflorine