title: LppM impact on the colonization of macrophages by Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis produces several bacterial effectors impacting the colonization of phagocytes. Here, we report that the putative lipoprotein LppM hinders phagocytosis by macrophages in a toll-like receptor 2-dependent manner. Moreover, recombinant LppM is able to functionally complement the phenotype of the mutant, when exogenously added during macrophage infection. LppM is also implicated in the phagosomal maturation, as a lppM deletion mutant is more easily addressed towards the acidified compartments of the macrophage than its isogenic parental strain. In addition, this mutant was affected in its ability to induce the secretion of pro-inflammatory chemokines, interferon-gamma-inducible protein-10, monocyte chemoattractant protein-1 and macrophage inflammatory protein-1α. Thus, our results describe a new mycobacterial protein involved in the early trafficking of the tubercle bacillus and its manipulation of the host immune response.
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Deboosère
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Iantomasi
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Christophe J
authors.initials: CJ
authors.lastname: Queval
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Ok-Ryul
authors.initials: OR
authors.lastname: Song
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Gaspard
authors.initials: G
authors.lastname: Deloison
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Jouny
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Debrie
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Mathias
authors.initials: M
authors.lastname: Chamaillard
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse CNRS, Toulouse, F-31077, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Nigou
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR 5048, Inserm U1054, Université de Montpellier, Montpellier, F-34090, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Cohen-Gonsaud
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Locht
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Priscille
authors.initials: P
authors.lastname: Brodin
authors.affiliation: Univ. Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, F-59000, France.|CNRS, UMR 8204, Lille, F-59000, France.|Inserm U1019, Lille, F-59000, France.|CHU Lille, Lille, F-59000, France.|Institut Pasteur de Lille, Lille, F-59000, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Veyron-Churlet
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D017353:Gene Deletion; D054884:Host-Pathogen Interactions; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology*; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000472:pathogenicity*; D010587:Phagocytosis; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; C000621620:LppM protein, Mycobacterium tuberculosis; D037521:Virulence Factors