title: Evaluation of technological properties of Enterococcus faecium CECT 8849, a strain isolated from human milk, for the dairy industry.
abstract: In this work, a variety of biochemical properties of Enterococcus faecium CECT 8849, which had been isolated from breast milk, were analyzed. Its acidifying capacity and proteolytic activity were low but, in contrast, remarkable peptidase and esterase activities were observed. Ethanol and 3-hydroxy-2-butanone were the most abundant volatile compounds found in experimental model cheese manufactured with E. faecium CECT 8849. This strain inhibited the growth of several Listeria monocytogenes and Listeria innocua strains in vitro. Enterocin A and B structural genes were detected in E. faecium CECT 8849. Model fermented milk and cheeses were manufactured from milk inoculated or not with L. innocua CECT 8848 (2.5-3 log10 colony forming units mL(-1)) using E. faecium CECT 8849 or Lactococcus lactis ESI 153 as starter cultures. Although E. faecium CECT 8849 controlled Listeria growth in both dairy models, it led to lower reduction in Listeria counts when compared with L. lactis ESI 153.
authors.affiliation: Probisearch, 28760 Tres Cantos, Madrid, Spain.
authors.forename: Nivia
authors.initials: N
authors.lastname: Cárdenas
authors.affiliation: Probisearch, 28760 Tres Cantos, Madrid, Spain.|Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Arroyo
authors.affiliation: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Carretera de La Coruña, km. 7.5, 28040, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Calzada
authors.affiliation: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Carretera de La Coruña, km. 7.5, 28040, Madrid, Spain.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Peirotén
authors.affiliation: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Carretera de La Coruña, km. 7.5, 28040, Madrid, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Medina
authors.affiliation: Probisearch, 28760 Tres Cantos, Madrid, Spain.|Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain.
authors.forename: Juan Miguel
authors.initials: JM
authors.lastname: Rodríguez
authors.affiliation: Probisearch, 28760 Tres Cantos, Madrid, Spain. leonides@ucm.es.|Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain. leonides@ucm.es.
authors.forename: Leonides
authors.initials: L
authors.lastname: Fernández
mesh_terms: D000093:Acetoin / Q000378:metabolism; D001430:Bacteriocins / Q000235:genetics; D002611:Cheese / Q000382:microbiology*; D043302:Cultured Milk Products / Q000382:microbiology*; D003612:Dairying; D016984:Enterococcus faecium / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis; D005516:Food Microbiology; D006801:Humans; D013294:Lactococcus lactis / Q000378:metabolism; D008089:Listeria monocytogenes / Q000254:growth & development; D008895:Milk, Human / Q000382:microbiology; D010447:Peptide Hydrolases / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D001430:Bacteriocins; D055549:Volatile Organic Compounds; C107799:enterocin A; C107801:enterocin B; D000431:Ethanol; D000093:Acetoin; D004950:Esterases; D010447:Peptide Hydrolases
keywords: Dairy models; Enterococcus faecium; Human milk; Listeria; Technological properties