title: Pseudomonas aeruginosa mannose-sensitive hemagglutinin inhibits proliferation and invasion via the PTEN/AKT pathway in HeLa cells.
abstract: We investigated the effects of Pseudomonas aeruginosa mannose-sensitive hemagglutinin (PA-MSHA) on the proliferation and invasion of human cervical cancer cell lines, as well as the molecular pathways underlying these effects. MTT cell proliferation assays revealed a time- and concentration-dependent cytotoxic effect of PA-MSHA on HeLa cells but not H8 cells. Flow cytometry with propidium iodide and annexin-V-fluorescein isothiocyanate labeling (FITC) indicated that various concentrations of PA-MSHA could induce apoptosis and G2-M cell cycle arrest in HeLa cells. PA-MSHA also impaired the migration and invasion abilities of HeLa cells in Wound healing and Transwell invasion assays. Western blot results demonstrated that PA-MSHA reduced the expression of p-AKT, p-GSK3β, BCL-2, Vimentin and β-catenin, but increased the levels of PTEN, BAD, BAX and E-cadherin in HeLa cells. Importantly, PTEN siRNA induced the activity of p-AKT, while PA-MSHA partly inhibited this induction, indicating that PA-MSHA may reduce the cell proliferation and invasion potential by activating PTEN and thus inhibiting the AKT pathway in vitro. These data suggest the potential application of PA-MSHA to the treatment of human cervical cancer.
authors.affiliation: The Second People's Hospital of Hunan Province, Clinical Medical College of Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Tie-Qiu
authors.initials: TQ
authors.lastname: Yin
authors.affiliation: The Second People's Hospital of Hunan Province, Clinical Medical College of Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Ou-Yang
authors.affiliation: The Second People's Hospital of Hunan Province, Clinical Medical College of Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Fang-Yan
authors.initials: FY
authors.lastname: Jiao
authors.affiliation: The Second People's Hospital of Hunan Province, Clinical Medical College of Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Huang
authors.affiliation: The Second People's Hospital of Hunan Province, Clinical Medical College of Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Tang
authors.affiliation: The Second People's Hospital of Hunan Province, Clinical Medical College of Hunan University of Chinese Medicine, Changsha 410007, China.
authors.forename: Bi-Qiong
authors.initials: BQ
authors.lastname: Ren
mesh_terms: D015703:Antigens, CD; D017209:Apoptosis / Q000187:drug effects; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D036781:Fimbriae Proteins / Q000494:pharmacology*; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008358:Mannose; D009361:Neoplasm Invasiveness; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014746:Vimentin / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C494819:BAD protein, human; C494826:BAX protein, human; C000595707:BCL2 protein, human; C513384:CDH1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014746:Vimentin; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D051176:beta Catenin; C000603452:mannosesensitive haemagglutinin, Pseudomonas aeruginosa; D036781:Fimbriae Proteins; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D008358:Mannose
keywords: PA-MSHA; PTEN; cervical cancer; p-AKT; p-GSK3β