title: Cellular folding pathway of a metastable serpin.
abstract: Although proteins generally fold to their thermodynamically most stable state, some metastable proteins populate higher free energy states. Conformational changes from metastable higher free energy states to lower free energy states with greater stability can then generate the work required to perform physiologically important functions. However, how metastable proteins fold to these higher free energy states in the cell and avoid more stable but inactive conformations is poorly understood. The serpin family of metastable protease inhibitors uses large conformational changes that are downhill in free energy to inhibit target proteases by pulling apart the protease active site. The serpin antithrombin III (ATIII) targets thrombin and other proteases involved in blood coagulation, and ATIII misfolding can thus lead to thrombosis and other diseases. ATIII has three disulfide bonds, two near the N terminus and one near the C terminus. Our studies of ATIII in-cell folding reveal a surprising, biased order of disulfide bond formation, with early formation of the C-terminal disulfide, before formation of the N-terminal disulfides, critical for folding to the active, metastable state. Early folding of the predominantly β-sheet ATIII domain in this two-domain protein constrains the reactive center loop (RCL), which contains the protease-binding site, ensuring that the RCL remains accessible. N-linked glycans and carbohydrate-binding molecular chaperones contribute to the efficient folding and secretion of functional ATIII. The inability of a number of disease-associated ATIII variants to navigate the folding reaction helps to explain their disease phenotypes.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003;
authors.forename: Kshama
authors.initials: K
authors.lastname: Chandrasekhar
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003;
authors.forename: Haiping
authors.initials: H
authors.lastname: Ke
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003; Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003;
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003;
authors.forename: Theresa
authors.initials: T
authors.lastname: Goodwin
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003; Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003; Department of Chemistry, University of Massachusetts, Amherst, MA 01003.
authors.forename: Lila M
authors.initials: LM
authors.lastname: Gierasch
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003; Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003;
authors.forename: Anne
authors.initials: A
authors.lastname: Gershenson
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003; Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003; dhebert@biochem.umass.edu.
authors.forename: Daniel N
authors.initials: DN
authors.lastname: Hebert
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000737:chemistry* / Q000235:genetics; D016466:CHO Cells; D003412:Cricetulus; D004220:Disulfides / Q000737:chemistry; D006801:Humans; D009154:Mutation; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D004220:Disulfides; D000990:Antithrombin III
keywords: antithrombin; cellular protein folding; endoplasmic reticulum; serpins; thrombosis