title: Simultaneous dual syringe electrospinning system using benign solvent to fabricate nanofibrous P(3HB-co-4HB)/collagen peptides construct as potential leave-on wound dressing.
abstract: The main focus of this study is the incorporation of collagen peptides to fabricate P(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] nano-fiber construct to further enhance surface wettability and support cell growth while harbouring desired properties for biodegradable wound dressing. Simultaneous electrospinning of nanofiber P(3HB-co-4HB)/collagen peptides construct was carried out using dual syringe system. The wettability of the constructs increased with the increase in 4HB molar fraction from 20mol% 4HB [53.2°], P(3HB-co-35mol%4HB)[48.9°], P(3HB-co-50mol%4HB)[44.5°] and P(3HB-co-82mol%4HB) [37.7°]. In vitro study carried out using mouse fibroblast cells (L929) grown on nanofiber P(3HB-co-4HB)/collagen peptides construct showed an increase in cell proliferation. In vivo study using animal model (Sprague Dawley rats) showed that nanofibrous P(3HB-co-4HB)/collagen peptides construct had a significant effect on wound contractions with the highest percentage of wound closure of 79%. Hence, P(3HB-co-4HB)/collagen peptides construct suitable for wound dressing have been developed using nano-fabrication technique.
authors.affiliation: Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, MOSTI, 11700 Penang, Malaysia; Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia.
authors.forename: S
authors.initials: S
authors.lastname: Vigneswari
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11700 Penang, Malaysia.
authors.forename: V
authors.initials: V
authors.lastname: Murugaiyah
authors.affiliation: Institute of Research in Molecular Medicine, Universiti Sains Malaysia, 11700 Penang, Malaysia.
authors.forename: G
authors.initials: G
authors.lastname: Kaur
authors.affiliation: School of Industrial Technology, Universiti Sains Malaysia, 11700 Penang, Malaysia.
authors.forename: H P S
authors.initials: HPS
authors.lastname: Abdul Khalil
authors.affiliation: Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, MOSTI, 11700 Penang, Malaysia; School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia; Centre of Chemical Biology, Universiti Sains Malaysia, 11900 Penang, Malaysia. Electronic address: amirul@usm.my.
authors.forename: A A
authors.initials: AA
authors.lastname: Amirul
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D006885:Hydroxybutyrates / Q000737:chemistry*; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared; D017155:Wettability; D014945:Wound Healing / Q000187:drug effects; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D003094:Collagen; D006885:Hydroxybutyrates; D011091:Polyesters; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates
keywords: Benign solvent; Collagen peptides; Electrospinning; P(3HB-co-4HB); Wound dressing