title: The effect of a specific histidine-rich glycoprotein polymorphism on male infertility and semen parameters.
abstract: In women, there is evidence that a single nucleotide polymorphism (SNP) in the histidine-rich glycoprotein (HRG) named HRG C633T is relevant for a number of fertility outcomes including recurrent miscarriage, ovarian response and pregnancy outcome after IVF. This case-control study was designed to investigate whether the HRG C633T SNP is important for male infertility and pregnancy rate following IVF. Cases were 139 infertile couples and controls were 196 pregnant couples. The 335 couples all contributed with one blood sample per partner. Genomic DNA was extracted and genotyping was performed using a TaqMan® SNP Genotyping Assay. Information on pregnancy rate and semen parameters was derived from medical records. Infertile couples in which the male partner was a homozygous carrier of the HRG C633T SNP had significantly lower (P < 0.01) pregnancy rate following IVF in comparison with couples where the male partner was a heterozygous HRG C633T SNP carrier. Male homozygous HRG 633T SNP carriers had overall lower total sperm count, sperm concentration, motility score and yield after preparation. In conclusion, once infertility is established the HRG C633T SNP seems to be important for male infertility and pregnancy rate following IVF.
authors.affiliation: Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden. Electronic address: karin.lindgren@kbh.uu.se.
authors.forename: Karin E
authors.initials: KE
authors.lastname: Lindgren
authors.affiliation: Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden; Fertilitetscentrum, Storängsvägen 10, SE-11542 Stockholm, Sweden.
authors.forename: Sarah
authors.initials: S
authors.lastname: Nordqvist
authors.affiliation: Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Kårehed
authors.affiliation: Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden.
authors.forename: Inger
authors.initials: I
authors.lastname: Sundström-Poromaa
authors.affiliation: Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden.
authors.forename: Helena
authors.initials: H
authors.lastname: Åkerud
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics; D000328:Adult; D000483:Alleles; D005260:Female; D005307:Fertilization in Vitro; D005838:Genotype; D006720:Homozygote; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011506:Proteins / Q000235:genetics*; D012661:Semen / Q000502:physiology*; D013076:Sperm Count; D000099053:Histidine-Rich Glycoprotein
chemical_list: D011506:Proteins; D000099053:Histidine-Rich Glycoprotein
keywords: angiogenesis; histidine-rich glycoprotein; infertile couples; male infertility; semen parameters; single nucleotide polymorphism