title: White stripe leaf 12(WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativaL.).
abstract: Chloroplast is a crucial organelle for plant photosynthesis and maintaining normal life activities in higher plants. Although some genes related to chloroplast development and pigment synthesis have been identified or cloned in rice, little is known about the relationship between these genes and abiotic stress response. In this study, we identified a novel mutantwhite stripe leaf 12(wsl12) affecting pigment synthesis, chloroplast development and abiotic stress response in rice. The mutant phenotype was obvious at seeding and tillering stages and in response to the temperature change. Genetic analysis of reciprocal crosses betweenwsl12and wild-type plants showed thatwsl12was a recessive mutant in a single nuclear locus. Map-based cloning revealed that theWSL12locus encoded OsNDPK2, one of the three nucleoside diphosphate kinases (OsNDPKs).WSL12expressed in all tested tissues, while it highly expressed in leaves and young tissues. The WSL12 protein localized to the chloroplast. Thewsl12mutant showed higher superoxide anion level and enhanced sensitivity to abscisic acid (ABA) and salinity. The transcription pattern of many genes involved in chlorophyll biosynthesis, ABA synthesis, light signaling pathway, reactive oxygen species-scavenging pathway and the other twoOsNDPKswas altered in thewsl12mutant. These results indicate that the OsNDPK2 encoded byWSL12plays an important role in chloroplast development and chlorophyll biosynthesis by regulating the expression levels of related genes. In addition,WSL12also affects the response to abiotic stress, such as ABA and salinity in rice, and is beneficial to molecular breeding of stress tolerance.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.|College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058 China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.|Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Shenzhen, 518120 China.
authors.forename: Shikai
authors.initials: S
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.|Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Shenzhen, 518120 China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Tiyuchang Road 359, Hangzhou, 310006 China.
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
keywords: Abiotic stress; Chloroplast development; Map-based cloning; Rice; WSL12