title: In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil.
abstract: Chemotherapy treatments induce a number of side effects, such as leukopenia neutropenia, peripheral erythropenia, and thrombocytopenia, affecting the quality of life for cancer patients. 5-Fluorouracil (5-FU) is wieldy used as myeloablative model in mice. The bovine dialyzable leukocyte extract (bDLE) or IMMUNEPOTENT CRP® (ICRP) is an immunomodulatory compound that has antioxidants and anti-inflammatory effects. In order to investigate the chemoprotection effect of ICRP on bone marrow cells in 5-FU treated mice, total bone marrow (BM) cell count, bone marrow colony forming units-granulocyte/macrophage (CFU-GM), cell cycle, immunophenotypification, ROS/superoxide and Nrf2 by flow cytometry, and histological and hematological analyses were performed. Our results demonstrated that ICRP increased BM cell count and CFU-GM number, arrested BM cells in G0/G1 phase, increased the percentage of leukocyte, granulocytic, and erythroid populations, reduced ROS/superoxide formation and Nrf2 activation, and also improved hematological levels and weight gain in 5-FU treated mice. These results suggest that ICRP has a chemoprotective effect against 5-FU in BM cells that can be used in cancer patients.
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Erika Evangelina
authors.initials: EE
authors.lastname: Coronado-Cerda
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Moisés Armides
authors.initials: MA
authors.lastname: Franco-Molina
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Mendoza-Gamboa
authors.affiliation: Departamento de Enfermedades Crónico-Degenerativas, Instituto Nacional de Enfermedades Respiratorias, Tlalpan 4502, Colonia Sección XVI, 14080 Ciudad de México, DF, Mexico.
authors.forename: Heriberto
authors.initials: H
authors.lastname: Prado-García
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Lydia Guadalupe
authors.initials: LG
authors.lastname: Rivera-Morales
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Pablo
authors.initials: P
authors.lastname: Zapata-Benavides
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Rodríguez-Salazar
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Diana
authors.initials: D
authors.lastname: Caballero-Hernandez
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Reyes Silvestre
authors.initials: RS
authors.lastname: Tamez-Guerra
authors.affiliation: Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), P.O. Box 46 "F", 66455 San Nicolás de los Garza, NL, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodríguez-Padilla
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001854:Bone Marrow Cells / Q000187:drug effects*; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D018890:Chemoprevention; D005472:Fluorouracil / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells; D007958:Leukocyte Count; D007962:Leukocytes / Q000737:chemistry*; D008297:Male; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000964:Antimetabolites, Antineoplastic; D001688:Biological Products; D051267:NF-E2-Related Factor 2; D020011:Protective Agents; D017382:Reactive Oxygen Species; D013481:Superoxides; D005472:Fluorouracil