title: Comparative Mitochondrial Genome Analysis of Eligma narcissus and other Lepidopteran Insects Reveals Conserved Mitochondrial Genome Organization and Phylogenetic Relationships.
abstract: In this study, we sequenced the complete mitochondrial genome of Eligma narcissus and compared it with 18 other lepidopteran species. The mitochondrial genome (mitogenome) was a circular molecule of 15,376 bp containing 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and an adenine (A) + thymine (T) - rich region. The positive AT skew (0.007) indicated the occurrence of more As than Ts. The arrangement of 13 PCGs was similar to that of other sequenced lepidopterans. All PCGs were initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which was initiated by the CGA sequence, as observed in other lepidopterans. The results of the codon usage analysis indicated that Asn, Ile, Leu, Tyr and Phe were the five most frequent amino acids. All tRNA genes were shown to be folded into the expected typical cloverleaf structure observed for mitochondrial tRNA genes. Phylogenetic relationships were analyzed based on the nucleotide sequences of 13 PCGs from other insect mitogenomes, which confirmed that E. narcissus is a member of the Noctuidae superfamily.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
authors.forename: Li-Shang
authors.initials: LS
authors.lastname: Dai
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
authors.forename: Cong-Fen
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Liu
mesh_terms: D020856:AT Rich Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D017124:Conserved Sequence; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D055029:Inverted Repeat Sequences; D024101:Mitochondrial Proteins / Q000235:genetics; D058977:Molecular Sequence Annotation; D009036:Moths / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D000073336:Whole Genome Sequencing
chemical_list: D003062:Codon; D019476:Insect Proteins; D024101:Mitochondrial Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer