title: Analysis of Methionine Oxidation in Myosin Isoforms in Porcine Skeletal Muscle by LC-MS/MS Analysis.
abstract: The purpose of this study was to analyze oxidized methionines in the myosin isoforms of porcine longissimus thoracis, psoas major, and semimembranosus muscles by liquid chromatography (LC) and mass spectrometry (MS). A total of 836 queries matched to four myosin isoforms (myosin-1, -2, -4, and -7) were analyzed and each myosin isoform was identified by its unique peptides (7.3-13.3). Forty-four peptides were observed from all three muscles. Seventeen peptides were unique to the myosin isoform and the others were common peptides expressed in two or more myosin isoforms. Five were identified as oxidized peptides with one or two methionine sulfoxides with 16 amu of mass modification. Methionines on residues 215 (215), 438 (438), 853 (851), 856 (854), 1071 (1069), and 1106 (1104) of myosin-1 (myosin-4) were oxidized by the addition of oxygen. Myosin-2 had two oxidized methionines on residues 215 and 438. No queries matched to myosin-7 were observed as oxidized peptides. LC-MS/MS allows analysis of the oxidation of specific amino acids on specific residue sites, as well as in specific proteins in the food system.
authors.affiliation: Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52852, Korea.
authors.forename: Jin-Yeon
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Division of Applied Life Science (BK21 plus), Gyeongsang National University, Jinju 52852, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Jung
authors.affiliation: Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52852, Korea.
authors.forename: Tae-Chul
authors.initials: TC
authors.lastname: Jeong
authors.affiliation: Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52852, Korea; Division of Applied Life Science (BK21 plus), Gyeongsang National University, Jinju 52852, Korea.
authors.forename: Han-Sul
authors.initials: HS
authors.lastname: Yang
authors.affiliation: Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52852, Korea; Department of Animal Sciences, University of Illinois at Urbana-Champaign, IL 61801, USA.
authors.forename: Gap-Don
authors.initials: GD
authors.lastname: Kim
keywords: LC-MS/MS; methionine; myosin; protein oxidation