title: Comparative Profiling of microRNA Expression in Soybean Seeds from Genetically Modified Plants and their Near-Isogenic Parental Lines.
abstract: MicroRNAs (miRNAs) have been widely demonstrated to play fundamental roles in gene regulation in most eukaryotes. To date, there has been no study describing the miRNA composition in genetically modified organisms (GMOs). In this study, small RNAs from dry seeds of two GM soybean lines and their parental cultivars were investigated using deep sequencing technology and bioinformatic approaches. As a result, several differentially expressed gma-miRNAs were found between the GM and non-GM soybeans. Meanwhile, more differentially expressed gma-miRNAs were identified between distantly relatednon-GM soybeans, indicating that the miRNA components of soybean seeds varied among different soybean lines, including the GM and non-GM soybeans, and the extent of difference might be related to their genetic relationship. Additionally, fourteen novel gma-miRNA candidates were predicted in soybean seeds including a potential bidirectionally transcribed miRNA family with two genomic loci (gma-miR-N1). Our findings firstly provided useful data for miRNA composition in edible GM crops and also provided valuable information for soybean miRNA research.
authors.affiliation: College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.|Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
authors.forename: Qingkuo
authors.initials: Q
authors.lastname: Lan
authors.affiliation: Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.
authors.forename: Feiwu
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, China.
authors.forename: Qinying
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
mesh_terms: D002523:Edible Grain / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D012639:Seeds / Q000235:genetics; D013025:Glycine max / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant