title: [Comprehensive Identification of MicroRNAs Regulated by Long Non-coding RNA MALAT1].
abstract: BACKGROUND
  Long non-coding RNA (lncRNA) plays important regulatory roles in the development and invasion of various cancers. The aim of current study is to comprehensively identify microRNAs (miRNA) regulated by lncRNA MALAT1 via experimental and bioinformatics methods.
  
  
  METHODS
  Antisense oligonucleotides (ASO) specifically targeting MALAT1 were designed and synthesized. After knockdown of MALAT1 by ASO in A549 cells, miRNA expression changes were profiled by TqaMan Low Density Array (TLDA). Gene set enrichment analysis (GSEA) was used to search enriched miRNAs among differentially expressed genes after knockdown of MALAT1.
  
  
  RESULTS
  After efficient knockdown of MALAT1 by ASO, 153 miRNAs were differentially expressed, 131 up-regulated and 22 down-regulated. Among the 458 differentially expressed genes after MALAT1 silence, GSEA results revealed lots of enriched miRNAs. There were 28 overlapped miRNAs between TLDA and GSEA results, suggesting these 28 miRNAs are regulated by MALAT1.
  
  
  CONCLUSIONS
  This study comprehensively identified MALAT1 regulated miRNAs, providing resources for further research.
authors.affiliation: Department of Clinical Medicine, Xuzhou Medical University, Xuzhou 221004, China.
authors.forename: Haitian
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing 210009, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yin
authors.affiliation: Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing 210009, China.
authors.forename: Mantang
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing 210009, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
mesh_terms: D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*
chemical_list: C557041:MALAT1 long non-coding RNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding