title: MS5 Mediates Early Meiotic Progression and Its Natural Variants May Have Applications for Hybrid Production in Brassica napus.
abstract: During meiotic prophase I, chromatin undergoes dynamic changes to establish a structural basis for essential meiotic events. However, the mechanism that coordinates chromosome structure and meiotic progression remains poorly understood in plants. Here, we characterized a spontaneous sterile mutant MS5(b)MS5(b) in oilseed rape (Brassica napus) and found its meiotic chromosomes were arrested at leptotene. MS5 is preferentially expressed in reproductive organs and encodes a Brassica-specific protein carrying conserved coiled-coil and DUF626 domains with unknown function. MS5 is essential for pairing of homologs in meiosis, but not necessary for the initiation of DNA double-strand breaks. The distribution of the axis element-associated protein ASY1 occurs independently of MS5, but localization of the meiotic cohesion subunit SYN1 requires functional MS5. Furthermore, both the central element of the synaptonemal complex and the recombination element do not properly form in MS5(b)MS5(b) mutants. Our results demonstrate that MS5 participates in progression of meiosis during early prophase I and its allelic variants lead to differences in fertility, which may provide a promising strategy for pollination control for heterosis breeding.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xin
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China College of Life Science, South-central University for Nationalities, Wuhan 430074, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Faming
authors.initials: F
authors.lastname: Dong
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China dfhong@mail.hzau.edu.cn zkcheng@genetics.ac.cn.
authors.forename: Dengfeng
authors.identifier: 0000-0001-9460-219X
authors.initials: D
authors.lastname: Hong
authors.affiliation: State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China dfhong@mail.hzau.edu.cn zkcheng@genetics.ac.cn.
authors.forename: Zhukuan
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D029688:Brassica napus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D032461:Chromosomes, Plant / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011418:Prophase / Q000235:genetics; D013573:Synaptonemal Complex / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins