title: Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures.
abstract: Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on subsequent introduction of 0.2 mg/l TDZ into the Kn-supplemented media, giving rise to somatic embryos. The embryogenic potential of calli expressed in terms of embryo-forming capacity (EFC) increased from 8.15 EFC to 20.95 EFC after plasmolysis. The phytochemical analysis (HPLC) for the presence of scopoletin and scoparone has revealed a unique accumulation pattern, with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development. The embryogenic calli contained the highest amount of coumarins (99.20 ± 0.97 and 61.03 ± 0.47 μg/gFW, respectively) followed by regenerated plant (9.43 ± 0.20 and 36.36 ± 1.19 μg/gFW, respectively), obtained via somatic embryogenesis. Rapid multiplication of A. indicum equipped with two potent coumarins is important in order to meet the commercial demand for combat against dreadful diseases, thereby providing a new platform for plant-based drugs and their manufacture on a commercial scale.
authors.affiliation: Department of Genetics, Faculty of Science, Osmania University, Hyderabad, 500 007, India. raorskiran@rediffmail.com.
authors.forename: Kiranmayee
authors.initials: K
authors.lastname: Rao
authors.affiliation: Department of Genetics, Faculty of Science, Osmania University, Hyderabad, 500 007, India.
authors.forename: Bhuvaneswari
authors.initials: B
authors.lastname: Chodisetti
authors.affiliation: Centre for Biotechnology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India.
authors.forename: Suryakala
authors.initials: S
authors.lastname: Gandi
authors.affiliation: Centre for Biotechnology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Giri
authors.affiliation: Department of Genetics, Faculty of Science, Osmania University, Hyderabad, 500 007, India.
authors.forename: P B Kavi
authors.initials: PB
authors.lastname: Kishor
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis*; D003583:Cytokinins / Q000494:pharmacology; D019660:Malvaceae / Q000737:chemistry*; D058439:Plant Somatic Embryogenesis Techniques; D012038:Regeneration / Q000187:drug effects; D012603:Scopoletin / Q000032:analysis*; D012639:Seeds / Q000187:drug effects; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003374:Coumarins; D003583:Cytokinins; C018145:scoparone; D012603:Scopoletin
keywords: A. indicum; Coumarins; HPLC; Scoparone; Scopoletin; Somatic embryogenesis