title: Simple and sensitive microbial pathogen detection using a label-free DNA amplification assay.
abstract: By the combination of quaternized magnetic nanoparticles and a label-free exonuclease III-assisted DNA amplification assay, we report a simple and facile strategy for the convenient and highly sensitive detection of microbial pathogens, with a detection limit of down to 50 cells mL(-1).
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. xqu@ciac.ac.cn.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Chuanqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Zhengqing
authors.initials: Z
authors.lastname: Yan
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D015374:Biosensing Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification*; D005090:Exodeoxyribonucleases / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D013211:Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D058185:Magnetite Nanoparticles; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III