title: Fibroproliferative effect of microRNA-21 in hypertrophic scar derived fibroblasts.
abstract: Hypertrophic scar (HS) is a fibroproliferative disorder caused by abnormal wound healing, which is characterized by excessive deposition of extracellular matrix (ECM) secreted by fibroblasts. We previous have found that expression of microRNA-21(miR-21) was increased in tissues and fibroblasts of HS. However, the underlying molecular mechanism remains to be further elucidated. In this study, we identified the miR-21 was a marker for the phenotype of HS fibroblasts, as anti-miR-21 reduced expression of fibrosis markers such as Col1A1, Col3A1, Fn and α-SMA in fibroblasts and overexpression of miR-21 promoted fibroproliferative expression in fibroblasts. Furthermore, we also found that miR-21 promoted TGF-β1 induced fibroproliferative expression by repressing Smad7 expression in vitro. In addition, the miR-21 inhibitor inhibited the growth of hypertrophic scar tissue in vivo (nude mice experimental model). These results indicated that miR-21 was a critical regulator for HS formation and TGF- β1/miR-21/Smad7 pathway could be a useful therapeutic target for the treatment of HS.
authors.affiliation: Department of Plastic Surgery, 1st Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, Anhui 233000, PR China.
authors.forename: Guangzao
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, PR China.
authors.forename: Renpeng
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of Burn and Plastic Surgery, People's Hospital of Dancheng County, 42 Ren Min Road, Zhoukou City, Henan Province 477150, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Plastic Surgery, 1st Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, Anhui 233000, PR China.
authors.forename: Banghong
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China. Electronic address: wuqingkai@sjtu.edu.cn.
authors.forename: Qingkai
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, PR China. Electronic address: wangchen1075@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D017439:Cicatrix, Hypertrophic / Q000235:genetics* / Q000473:pathology*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051906:Smad7 Protein / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D051906:Smad7 Protein; D053773:Transforming Growth Factor beta1
keywords: Fibrosis; Hypertrophic scar; MiR-21; Smad7; TGF-β1