title: [Structure and Activity of Fungal Lipases in Bile Salt Solutions].
abstract: The changes in structure and catalytic properties of fungal lipases (Candida rugosa, Rhizomucor miehei, Mucor javanicus) were investigated in micellar solutions of bile salts that differ in hydrophilic-lypophilic balance and reaction medium properties. The methods of circular dichroism and tryptophan fluorescence were applied to estimate the changes in peptide structure within complexes with bile salt micelles. Bile salts do not exert a significant influence on the structure of the enzymes under study: in Rh. miehei and M. javanicus lipases the alpha helix content slightly decreased, the influence of bile salts on the C. rugosa structure was not revealed. Despite negligible structural modifications in the enzymes, in bile salt solutions a considerable change in their catalytic properties was observed: an abrupt decrease in catalytic effectiveness. Substrate-bile salts micelles complex formation was demonstrated by the NMR self-diffusion method. The model of a regulation of fungal lipase activity was proposed.
authors.forename: L R
authors.initials: LR
authors.lastname: Bogdanova
authors.forename: D R
authors.initials: DR
authors.lastname: Bakirova
authors.forename: Yu A
authors.initials: YA
authors.lastname: Valiullina
authors.forename: B Z
authors.initials: BZ
authors.lastname: Idiyatullin
authors.forename: D A
authors.initials: DA
authors.lastname: Faizullin
authors.forename: O S
authors.initials: OS
authors.lastname: Zueva
authors.forename: Yu F
authors.initials: YF
authors.lastname: Zuev
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry*; D002175:Candida / Q000201:enzymology; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D009089:Mucor / Q000201:enzymology; D019906:Nuclear Magnetic Resonance, Biomolecular; D020103:Rhizomucor / Q000201:enzymology; D012996:Solutions / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D001647:Bile Acids and Salts; D012996:Solutions; D008049:Lipase