title: Immunohistochemical expression of budding uninhibited by benzimidazole related 1 in leukoplakia and oral squamous cell carcinoma.
abstract: BACKGROUND
  Budding uninhibited by benzimidazole related 1 (BUBR1) is an important protein in the mitotic spindle assembly checkpoint. Alterations in expression of BUBR1 have been reported in many premalignant and malignant lesions.
  
  
  OBJECTIVE
  To compare the expression of BUBR1 with respect to the normal mucosa and degree of dysplasia in oral leukoplakia (OL) and also with respect to different histopathological grades of oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  Neutral buffered formalin-fixed and paraffin-embedded biopsy specimens 30 each of normal, OL and OSCC tissue were included in this study. The expression of BUBR1 was detected using immunohistochemistry (IHC). The scores obtained were subjected to ANOVA test.
  
  
  RESULTS
  Significant correlation was found in immunostaining between normal, dysplasia and OSCC groups with a P value of 0.00001. The expression of BUBR1 was significant when compared with different degrees of dysplasia and in different histopathological grades of OSCC with a P value of 0.00001.
  
  
  CONCLUSIONS
  Higher IHC scores were obtained with increased histopathological grades of OL and OSCC suggesting its role as a prognostic indicator.
authors.affiliation: Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
authors.forename: Taneeru
authors.initials: T
authors.lastname: Sravya
authors.affiliation: Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
authors.forename: Yeluri
authors.initials: Y
authors.lastname: Sivaranjani
authors.affiliation: Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
authors.forename: Guttikonda Venkateswara
authors.initials: GV
authors.lastname: Rao
keywords: Budding uninhibited by benzimidazole related 1; oral leukoplakia; oral squamous cell carcinoma