title: Characterization of an ene-reductase from Meyerozyma guilliermondii for asymmetric bioreduction of α,β-unsaturated compounds.
abstract: OBJECTIVE
  To characterize a novel ene-reductase from Meyerozyma guilliermondii and achieve the ene-reductase-mediated reduction of activated C=C bonds.
  
  
  RESULTS
  The gene encoding an ene-reductase was cloned from M. guilliermondii. Sequence homology analysis showed that MgER shared the maximal amino acid sequence identity of 57 % with OYE2.6 from Scheffersomyces stipitis. MgER showed the highest specific activity at 30 °C and pH 7 (100 mM sodium phosphate buffer), and excellent stereoselectivities were achieved for the reduction of (R)-carvone and ketoisophorone. Under the reaction conditions (30 °C and pH 7.0), 150 mM (R)-carvone could be completely converted to (2R,5R)-dihydrocarvone within 22 h employing purified MgER as catalyst, resulting in a yield of 98.9 % and an optical purity of >99 % d.e.
  
  
  CONCLUSIONS
  MgER was characterized as a novel ene-reductase from yeast and showed great potential for the asymmetric reduction of activated C=C bonds of α,β-unsaturated compounds.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Baoqi
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Liandan
authors.initials: L
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China. jplin@ecust.edu.cn.
authors.forename: Jinping
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D000081005:Cyclohexane Monoterpenes; D005656:Fungal Proteins / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D004718:Saccharomycetales / Q000201:enzymology* / Q000378:metabolism
chemical_list: D000081005:Cyclohexane Monoterpenes; D005656:Fungal Proteins; D039821:Monoterpenes; D010088:Oxidoreductases; D009252:NADPH Dehydrogenase; C556859:dihydrocarvone
keywords: (R)-Carvone; Bioreduction; Dihydrocarvone; Ene-reductase; NADPH dehydrogenase; Old yellow enzyme; α,β-Unsaturated compounds