title: HSI colour-coded analysis of scattered light of single plasmonic nanoparticles.
abstract: Single plasmonic nanoparticles (PNPs) analysis with dark-field microscopic imaging (iDFM) has attracted much attention in recent years. The ability for quantitative analysis of iDFM is critical, but cumbersome, for characterizing and analyzing the scattered light of single PNPs. Here, a simple automatic HSI colour coding method is established for coding dark-field microscopic (DFM) images of single PNPs with localized surface plasmon resonance (LSPR) scattered light, showing that hue value in the HSI system can realize accurate quantitative analysis of iDFM and providing a novel approach for quantitative chemical and biochemical imaging at the single nanoparticle level.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. chengzhi@swu.edu.cn and College of Computer and Information Science, Southwest University, Chongqing 400715, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. chengzhi@swu.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Lei
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. chengzhi@swu.edu.cn.
authors.forename: Lin Ling
authors.initials: LL
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. chengzhi@swu.edu.cn.
authors.forename: Peng Fei
authors.initials: PF
authors.lastname: Gao
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. chengzhi@swu.edu.cn and Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang