title: Inflammatory monocyte/macrophage modulation by liposome-entrapped spironolactone ameliorates acute lung injury in mice.
abstract: To examine the therapeutic/preventive potential of liposome-encapsulated spironolactone (SP; Lipo-SP) for acute lung injury (ALI) and fibrosis.
  
  
  
  Lipo-SP was prepared by the film-ultrasonic method, and physicochemical and pharmacokinetic characterized for oral administration (10 and 20 mg/kg for SP-loaded liposome; 20 mg/kg for free SP) in a mouse model bleomycin-induced ALI.
  
  
  
  Lipo-SP enhanced bioavailability of SP with significant amelioration in lung pathology. Mechanistically, SP-mediated mineralocorticoid receptor antagonism contributes to inflammatory monocyte/macrophage modulation via an inhibitory effect on Ly6C(hi) monocytosis-directed M2 polarization of alveolar macrophages. Moreover, Lipo-SP at lower dose (10 mg/kg) exhibited more improvement in body weight gain.
  
  
  
  Our data highlight Lipo-SP as a promising approach with therapeutic/preventive potential for ALI and fibrosis.
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.|Department of Respiratory & Critical Care Medicine, Pingjin Hospital, Tianjin 300162, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Ji
authors.affiliation: Department of Respiratory & Critical Care Medicine, Pingjin Hospital, Tianjin 300162, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Ma
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pharmacognosy & Pharmaceutics, Logistics University of People's Armed Police Forces, Tianjin 300309, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.
authors.forename: Yi-Dan
authors.initials: YD
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Su
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.
authors.forename: Guo-An
authors.initials: GA
authors.lastname: Xiang
authors.affiliation: Department of Pharmacognosy & Pharmaceutics, Logistics University of People's Armed Police Forces, Tianjin 300309, China.
authors.forename: Mei-Ping
authors.initials: MP
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.
authors.forename: Zhi-Chun
authors.initials: ZC
authors.lastname: Lin
authors.affiliation: Department of Respiratory & Critical Care Medicine, Pingjin Hospital, Tianjin 300162, China.
authors.forename: Lu-Qing
authors.initials: LQ
authors.lastname: Wei
authors.affiliation: Division of Community Health & Humanities, Faculty of Medicine, Memorial University of Newfoundland, Canada.
authors.forename: Peizhong P
authors.initials: PP
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.|Department of Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
authors.forename: Zhuoli
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001761:Bleomycin; D016764:Cell Polarity; D006801:Humans; D008081:Liposomes; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D000451:Mineralocorticoid Receptor Antagonists / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010316:Particle Size; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D013148:Spironolactone / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D008081:Liposomes; D000451:Mineralocorticoid Receptor Antagonists; D001761:Bleomycin; D013148:Spironolactone
keywords: acute lung injury; liposome; pulmonary fibrosis; spironolactone