title: Fluorescent probes for nanoscopy: four categories and multiple possibilities.
abstract: Nanoscopy enables breaking down the light diffraction limit and reveals the nanostructures of objects being studied using light. In 2014, three scientists pioneered the development of nanoscopy and won the Nobel Prize in Chemistry. This recognized the achievement of the past twenty years in the field of nanoscopy. However, fluorescent probes used in the field of nanoscopy are still numbered. Here, we review the currently available four categories of probes and existing methods to improve the performance of probes.
authors.affiliation: Fujian Provincial Key Laboratory for Photonics Technology & Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China.|Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore, 138669, Singapore.
authors.forename: Ming
authors.initials: M
authors.lastname: Ni
authors.affiliation: Fujian Provincial Key Laboratory for Photonics Technology & Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China.|Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #10-01 CREATE Towe, Singapore, 138602, Singapore.
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.affiliation: Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #10-01 CREATE Towe, Singapore, 138602, Singapore.|Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
authors.affiliation: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore, 138669, Singapore.|Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #10-01 CREATE Towe, Singapore, 138602, Singapore.|Department of Physiology, Yong Loo Lin School of Medicine, MD9-04-11, 2 Medical Drive, Singapore, 117597, Singapore.|Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore, 117411, Singapore.
authors.forename: Hanry
authors.initials: H
authors.lastname: Yu
mesh_terms: D005456:Fluorescent Dyes; D008027:Light; D049329:Nanostructures; D036103:Nanotechnology
chemical_list: D005456:Fluorescent Dyes
keywords: Fluorescent Proteins (FPs); Nanoparticles (NPs); Organic small molecules (OSMs); Quantum Dots (QDs); fluorescent probes; nanoscopy