title: Hepatotoxicity evaluation of dextran stabilized iron oxide nanoparticles in Wistar rats.
abstract: Cellular and organ responses to nanoparticles are relevant in the context of use of nanoparticles for biomedical applications. The purpose of the present study was to determine the potential of dextran stabilized iron oxide nanoparticles (DIONPs) to influence hepatic uptake and consequently induce hepatotoxic response in rats following intravenous administration. Inductively coupled plasma atomic emission spectroscopy analysis revealed that DIONPs are rapidly taken up into the liver, progressively broken down to iron constituents and exported into blood, with a part of it being retained in the liver. The potential of DIONPs to induce oxidative stress response was determined by evaluating the time-dependent redox defense status. Maximum alterations in antioxidant activities were observed to occur within a period of 7days. However, this effect was not followed by significant increase in lipid peroxidation or modulation of hepatic enzymes such as alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase and bilirubin levels. Overall, these data imply that the liver retains functional integrity with a dose of 10mg/kg DIONPs, although with brief activation of redox defenses.
authors.affiliation: Division of Toxicology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram 695 012, Kerala, India.
authors.forename: Sheeja Liza
authors.initials: SL
authors.lastname: Easo
authors.affiliation: Division of Toxicology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram 695 012, Kerala, India. Electronic address: mohanpv10@gmail.com.
authors.forename: P V
authors.initials: PV
authors.lastname: Mohanan
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003911:Dextrans / Q000009:adverse effects* / Q000737:chemistry; D005290:Ferric Compounds / Q000009:adverse effects* / Q000737:chemistry; D007501:Iron / Q000009:adverse effects* / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008297:Male; D053758:Nanoparticles / Q000009:adverse effects* / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D003911:Dextrans; D005290:Ferric Compounds; C000499:ferric oxide; D007501:Iron; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase
keywords: Antioxidant enzymes; Iron oxide nanoparticles; Lipid peroxidation; Liver; Oxidative stress