title: Preventive Effects of Spirogyra neglecta and a Polysaccharide Extract against Dextran Sodium Sulfate Induced Colitis in Mice.
abstract: Ulcerative colitis (UC) results from colonic epithelial barrier defects and impaired mucosal immune responses. In this study, we aimed to investigate the modifying effects of a Spirogyra neglecta extract (SNE), a polysaccharide extract (PE) and a chloroform fraction (CF) on dextran sodium sulfate (DSS)-induced colitis in mice and to determine the mechanisms. To induce colitis, ICR mice received 3% DSS in their drinking water for 7 days. Seven days preceding the DSS treatment, oral administration of SNE, PE and CF at doses of 50, 25 and 0.25 mg/kg body weight (low dose), 200, 100 and 1 mg/kg body weight (high dose) and vehicle was started and continued for 14 days. Histologic findings showed that DSS-induced damage of colonic epithelial structure and inflammation was attenuated in mice pre-treated with SNE, PE and CF. Furthermore, SNE and PE significantly protected colonic epithelial cells from DSS-induced cell cycle arrest, while SNE, PE and CF significantly diminished apoptosis. Proteome analysis demonstrated that SNE and PE might ameliorate DSS-induced colitis by inducing antioxidant enzymes, restoring impaired mitochondria function, and regulating inflammatory cytokines, proliferation and apoptosis. These results suggest that SNE and PE could prevent DSS-induced colitis in ICR mice by protection against and/or aiding recovery from damage to the colonic epithelium, reducing ROS and maintaining normal mitochondrial function and apoptosis.
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, Asahi-machi, Abeno-ku, Osaka, Japan E-mail : wani@ med.osaka-cu.ac.jp.
authors.forename: Sirinya
authors.initials: S
authors.lastname: Taya
authors.forename: Anna
authors.initials: A
authors.lastname: Kakehashi
authors.forename: Rawiwan
authors.initials: R
authors.lastname: Wongpoomchai
authors.forename: Min
authors.initials: M
authors.lastname: Gi
authors.forename: Naomi
authors.initials: N
authors.lastname: Ishii
authors.forename: Hideki
authors.initials: H
authors.lastname: Wanibuchi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002853:Chromatography, Liquid; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D016264:Dextran Sulfate / Q000633:toxicity*; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry*; D040901:Proteomics; D015398:Signal Transduction / Q000187:drug effects; D058131:Spirogyra / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D016264:Dextran Sulfate