title: Shape-Encoded Chitosan-Polyacrylamide Hybrid Hydrogel Microparticles with Controlled Macroporous Structures via Replica Molding for Programmable Biomacromolecular Conjugation.
abstract: Polymeric hydrogel microparticle-based suspension arrays with shape-based encoding offer powerful alternatives to planar and bead-based arrays toward high throughput biosensing and medical diagnostics. We report a simple and robust micromolding technique for polyacrylamide- (PAAm-) based biopolymeric-synthetic hybrid microparticles with controlled 2D shapes containing a potent aminopolysaccharide chitosan as an efficient conjugation handle uniformly incorporated in PAAm matrix. A postfabrication conjugation approach utilizing amine-reactive chemistries on the chitosan shows stable incorporation and retained chemical reactivity of chitosan, readily tunable macroporous structures via simple addition of low content long-chain PEG porogens for improved conjugation capacity and kinetics, and one-pot biomacromolecular assembly via bioorthogonal click reactions with minimal nonspecific binding. We believe that the integrated fabrication-conjugation approach reported here could offer promising routes to programmable manufacture of hydrogel microparticle-based biomacromolecular conjugation and biofunctionalization platforms for a large range of applications.
authors.affiliation: Department of Chemical and Biological Engineering, Tufts University , Medford, Massachusetts 02155 United States.
authors.forename: Eunae
authors.initials: E
authors.lastname: Kang
authors.affiliation: Department of Chemical and Biological Engineering, Tufts University , Medford, Massachusetts 02155 United States.
authors.forename: Sukwon
authors.initials: S
authors.lastname: Jung
authors.affiliation: Department of Chemical and Biological Engineering, Tufts University , Medford, Massachusetts 02155 United States.
authors.forename: John H
authors.initials: JH
authors.lastname: Abel
authors.affiliation: Department of Chemical and Biological Engineering, Tufts University , Medford, Massachusetts 02155 United States.
authors.forename: Allison
authors.initials: A
authors.lastname: Pine
authors.affiliation: Department of Chemical and Biological Engineering, Tufts University , Medford, Massachusetts 02155 United States.
authors.forename: Hyunmin
authors.initials: H
authors.lastname: Yi