title: Three important amino acids control the regioselectivity of flavonoid glucosidation in glycosyltransferase-1 from Bacillus cereus.
abstract: Glycosyltransferase-1 from Bacillus cereus (BcGT1) catalyzes a reaction that transfers a glucosyl moiety to flavonoids, such as quercetin, kaempferol, and myricetin. The enzymatic glucosidation shows a broad substrate specificity when the reaction is catalyzed by wild-type BcGT1. Preliminary assays demonstrated that the F240A mutant significantly improves the regioselectivity of enzymatic glucosidation toward quercetin. To unveil and further to control the catalytic function of BcGT1, mutation of F240 to other amino acids, such as C, E, G, R, Y, W, and K, was performed. Among these mutants, F240A, F240G, F240R, and F240K greatly altered the regioselectivity. The quercetin-3-O-glucoside, instead of quercetin-7-O-glucoside as for the wild-type enzyme, was obtained as the major product. Among these mutants, F240R showed nearly 100 % product specificity but only retained 25 % catalytic efficiency of wild-type enzyme. From an inspection of the protein structure, we found two other amino acids, F132 and F138, together with F240, are likely to form a hydrophobic binding region, which is sufficiently spacious to accommodate substrates with varied aromatic moieties. Through the replacement of a phenylalanine by a tyrosine residue in the substrate-binding region, the mutants may be able to fix the orientation of flavonoids, presumably through the formation of a hydrogen bond between substrates and mutants. Multiple mutants-F240R_F132Y, F240R_F138Y, and F240R_F132Y_F138Y-were thus constructed for further investigation. The multiple points of mutants not only maintained the high product specificity but also significantly improved the catalytic efficiency, relative to F240R. The same product specificity was obtained when kaempferol and myricetin were used as a substrate.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Science Building 2, 1001 Ta Hsueh Road, Hsinchu, 30010, Taiwan.
authors.forename: Hsi-Ho
authors.initials: HH
authors.lastname: Chiu
authors.affiliation: Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, 101 Hsin-Ann Road, Hsinchu, 30076, Taiwan.
authors.forename: Yin-Cheng
authors.initials: YC
authors.lastname: Hsieh
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Science Building 2, 1001 Ta Hsueh Road, Hsinchu, 30010, Taiwan.
authors.forename: Ya-Huei
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Degree Program of Applied Science and Technology, College of Science, National Chiao Tung University, Science Building 3, 1001 Ta Hsueh Road, Hsinchu, 30010, Taiwan.
authors.forename: Hsin-Ying
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Science Building 2, 1001 Ta Hsueh Road, Hsinchu, 30010, Taiwan.
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Lu
authors.affiliation: Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, 101 Hsin-Ann Road, Hsinchu, 30076, Taiwan. cjchen@nsrrc.org.tw.
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Science Building 2, 1001 Ta Hsueh Road, Hsinchu, 30010, Taiwan. ykl@faculty.nctu.edu.tw.
authors.forename: Yaw-Kuen
authors.initials: YK
authors.lastname: Li
mesh_terms: D019943:Amino Acid Substitution; D000596:Amino Acids / Q000235:genetics* / Q000378:metabolism*; D001409:Bacillus cereus / Q000201:enzymology*; D001665:Binding Sites; D005419:Flavonoids / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D005419:Flavonoids; D050505:Mutant Proteins; D016695:Glycosyltransferases
keywords: BcGT1; Flavonoid; Glucosidation; Glucosyltransferase; Regioselectivity