title: Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes.
abstract: The protective effect of different polyphenols, catechin (Cat), quercetin (Qc) (flavonoids), gallic acid (GA), caffeic acid (CfA), chlorogenic acid (ChA) (phenolic acids), and capsaicin (Cap), against H2O2-induced oxidative stress was evaluated in rat enterocytes using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) Spectroscopy and Fourier Transform Infrared Microspectroscopy (FTIRM), and results were compared to standard lipid peroxidation techniques: conjugated dienes (CD) and Thiobarbituric Acid Reactive Substances (TBARS). Analysis of ATR-FTIR and FTIRM spectral data allowed the simultaneous evaluation of the effects of H2O2 and polyphenols on lipid and protein oxidation. All polyphenols showed a protective effect against H2O2-induced oxidative stress in enterocytes, when administered before or after H2O2. Cat and capsaicin showed the highest protective effect, while phenolic acids had weaker effects and Qc presented a mild prooxidative effect (IR spectral profile of biomolecules between control and H2O2-treated cells) according to FTIR analyses. These results demonstrated the viability to use infrared spectroscopy to evaluate the oxidant and antioxidant effect of molecules in cell systems assays.
authors.affiliation: Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, 32310 Ciudad Juárez, CHIH, Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Barraza-Garza
authors.affiliation: X-ray and Infrared Microspectroscopy Beamline ID21, European Synchrotron Radiation Facility, BP 220, 38043 Grenoble Cedex, France.
authors.forename: Hiram
authors.initials: H
authors.lastname: Castillo-Michel
authors.affiliation: Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, 32310 Ciudad Juárez, CHIH, Mexico.
authors.forename: Laura A
authors.initials: LA
authors.lastname: de la Rosa
authors.affiliation: Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, 32310 Ciudad Juárez, CHIH, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martinez-Martinez
authors.affiliation: Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, 32310 Ciudad Juárez, CHIH, Mexico.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Pérez-León
authors.affiliation: X-ray and Infrared Microspectroscopy Beamline ID21, European Synchrotron Radiation Facility, BP 220, 38043 Grenoble Cedex, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Cotte
authors.affiliation: Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, 32310 Ciudad Juárez, CHIH, Mexico.
authors.forename: Emilio
authors.initials: E
authors.lastname: Alvarez-Parrilla
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D020895:Enterocytes / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity; D007422:Intestines / Q000166:cytology; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D059808:Polyphenols; D006861:Hydrogen Peroxide