title: Assessing Caspase-1 Activation.
abstract: The caspase-1 enzymatic activity plays a major role in the innate immune response as it regulates the maturation of two major proinflammatory cytokines, the interleukin-1beta (IL-1β) and IL-18. In this chapter, we describe the technique of Western blot to assess caspase-1 activation. This method provides multiple information within one experiment. It allows the detection of both unprocessed and processed caspase-1 and substrates.
authors.affiliation: INSERM U1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France.|CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France.|Université de Lyon, Lyon, France.|Centre Léon Bérard, Lyon, France.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Guey
authors.affiliation: INSERM U1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France. virginie.petrilli@lyon.unicancer.fr.|CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France. virginie.petrilli@lyon.unicancer.fr.|Université de Lyon, Lyon, France. virginie.petrilli@lyon.unicancer.fr.|Centre Léon Bérard, Lyon, France. virginie.petrilli@lyon.unicancer.fr.|Centre de Recherche en Cancérologie de Lyon, 28 rue Laennec, 69008, Lyon, France. virginie.petrilli@lyon.unicancer.fr.
authors.forename: Virginie
authors.initials: V
authors.lastname: Petrilli
mesh_terms: D000818:Animals; D015153:Blotting, Western; D020170:Caspase 1 / Q000378:metabolism*; D002478:Cells, Cultured; D007113:Immunity, Innate; D020382:Interleukin-18 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism; D051379:Mice
chemical_list: C587672:IL1B protein, mouse; D020382:Interleukin-18; D053583:Interleukin-1beta; D020170:Caspase 1
keywords: Caspase-1; Inflammasome; Interleukin-1beta (IL-1β); Western blot