title: PI3K/Akt Activated by GPR30 and Src Regulates 17β-Estradiol-Induced Cultured Immature Boar Sertoli Cells Proliferation.
abstract: Sertoli cell (SC) is a key element in the process of spermatogenesis. Accumulating research show that estrogen plays an important role in regulating boar SC proliferation. However, it is unclear whether phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B (PI3K/Akt) is involved in this process. In the present study, the role of PI3K/Akt on the 17β-estradiol-induced piglet SC proliferation was explored. In addition, we also explained the roles of G-protein-coupled estrogen receptor (GPR30) and Sarcoma protein (Src) in this process. Our study demonstrated that, 17β-estradiol induced activation of PI3K in a time-dependent manner. Both G-15 (an antagonist of GPR30, 0.1 μmol/L) and PP2 (an inhibitor of Src, 2.0 μmol/L) inhibited 17β-estradiol-induced activation of PI3K, reduced SC proliferation, and decreased messenger RNA (mRNA) and protein expression of S-phase kinase-associated protein 2 (Skp2). We also found that 17β-estradiol induced activation of Akt in a time-dependent manner. Both LY294002 (an inhibitor of PI3K) and 10-DEBC (an inhibitor of Akt) significantly reduced 17β-estradiol-induced SC proliferation and reduced mRNA and protein expression of Skp2. In addition, LY294002 inhibited 17β-estradiol-induced activation of Akt. The results indicated that 17β-estradiol regulates SC proliferation by activating PI3K/Akt. Both GPR30 and Src are involved in 17β-estradiol-induced phosphorylation of PI3K/Akt. Activation of PI3K/Akt enhances the expression of Skp2, which promotes SC proliferation.
authors.affiliation: 1 College of Animal Science and Technology, Southwest University, Chongqing, China.|2 Chongqing Key Laboratory of Forage & Herbivore, Chongqing, China.
authors.forename: Wei-Rong
authors.initials: WR
authors.lastname: Yang
authors.affiliation: 1 College of Animal Science and Technology, Southwest University, Chongqing, China.|2 Chongqing Key Laboratory of Forage & Herbivore, Chongqing, China.
authors.forename: Feng-Wei
authors.initials: FW
authors.lastname: Zhu
authors.affiliation: 1 College of Animal Science and Technology, Southwest University, Chongqing, China.|2 Chongqing Key Laboratory of Forage & Herbivore, Chongqing, China.
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: 1 College of Animal Science and Technology, Southwest University, Chongqing, China.|2 Chongqing Key Laboratory of Forage & Herbivore, Chongqing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 1 College of Animal Science and Technology, Southwest University, Chongqing, China.|2 Chongqing Key Laboratory of Forage & Herbivore, Chongqing, China.
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: 3 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.|4 Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.affiliation: 1 College of Animal Science and Technology, Southwest University, Chongqing, China.|2 Chongqing Key Laboratory of Forage & Herbivore, Chongqing, China.
authors.forename: Xian-Zhong
authors.initials: XZ
authors.lastname: Wang
keywords: 17β-estradiol; GPR30; PI3K/Akt; Sertoli cell; Src