title: Evaluation of changes in serum chemistry in association with feed withdrawal or high dose oral gavage with dextran sodium sulfate- (DSS-) induced gut leakage in broiler chickens.
abstract: Dextran sodium sulfate ( DSS: ) has been shown to be effective at inducing enteric inflammation in broiler chickens, resulting in increased leakage of orally administered fluorescein isothiocyanate dextran to circulation. In a previous study, 2 doses of DSS (0.45 g/dose) administered as oral gavage resulted in increased mucosal permeability. The main objective of the present study was to compare serum turbidity in control and DSS treated birds plus with feed restriction ( FR: ), and evaluate the associated serum chemistry. Three independent experiments were conducted with different combinations of treatment groups. In Experiment 1, control full-fed ( CON: ) and DSS full-fed ( FFD: ) with n = 15 birds/group were evaluated, Experiment 2 had groups (n = 15/group) CON, FFD, feed restriction ( FRS: for 34 h), and DSS with feed restriction ( FRD: ), and Experiment 3 (n = 15/group) had CON, FFD, and FRS (29 h FRS). All DSS treated birds received one or 2 doses of DSS by oral gavage (0.45 g/dose/bird). Results showed that, compared to CON group, there was an increase (P < 0.05) in serum turbidity in FFD birds, even though the difference between FRS and FRD was not apparent (P > 0.05). Administration of DSS did not result in increase of serum enzymes such as alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase ( LDH: ), nonetheless, the FFD showed lower (P < 0.05) LDH level compared to CON in Experiment 2. Among the various serum chemistry parameters evaluated triglycerides had the highest positive correlation (r2= 0.85; P < 0.05) with serum turbidity. DSS administration resulted in decreased serum protein levels, especially albumin. These results suggest that oral gavage with DSS in broiler chicks could result in changes to serum chemistry parameters which could be developed as potential marker/s for gut leakage.
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: V A
authors.initials: VA
authors.lastname: Kuttappan
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: E A
authors.initials: EA
authors.lastname: Vicuña
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: O B
authors.initials: OB
authors.lastname: Faulkner
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: G R
authors.initials: GR
authors.lastname: Huff
authors.affiliation: Pat Walker Health Center, University of Arkansas, Fayetteville, AR.
authors.forename: K A
authors.initials: KA
authors.lastname: Freeman
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: J D
authors.initials: JD
authors.lastname: Latorre
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: A
authors.initials: A
authors.lastname: Menconi
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: G I
authors.initials: GI
authors.lastname: Tellez
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR.
authors.forename: B M
authors.initials: BM
authors.lastname: Hargis
authors.affiliation: Department of Animal Science, The Ohio State University, Columbus, OH bielke.1@osu.edu.
authors.forename: L R
authors.initials: LR
authors.lastname: Bielke
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000662:veterinary; D002645:Chickens; D016264:Dextran Sulfate / Q000008:administration & dosage / Q000009:adverse effects*; D004032:Diet / Q000662:veterinary; D007249:Inflammation / Q000139:chemically induced / Q000175:diagnosis / Q000662:veterinary*; D009391:Nephelometry and Turbidimetry / Q000662:veterinary; D010539:Permeability; D011201:Poultry Diseases / Q000139:chemically induced / Q000175:diagnosis*; D011897:Random Allocation
chemical_list: D015415:Biomarkers; D016264:Dextran Sulfate
keywords: Dextran Sodium Sulfate; enteric inflammation; mucosal permeability; serum chemistry; triglycerides