title: Human induced pluripotent stem cell-derived fiber-shaped cardiac tissue on a chip.
abstract: We propose a method for the production of a fiber-shaped three-dimensional (3D) cellular construct of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) for the quantification of the contractile force. By culturing the cardiomyocytes in a patterned hydrogel structure with fixed edges, we succeeded in fabricating hiPS-CM fibers with aligned cardiomyocytes. The fiber generated contractile force along the fiber direction due to the hiPS-CM alignment, and we were able to measure its contractile force accurately. Furthermore, to demonstrate the drug reactivity of hiPS-CM fibers, the changes in the contractile frequency and force following treatment with isoproterenol and propranolol were observed. We believe that hiPS-CM fibers will be a useful tool for pharmacokinetic analyses during drug development.
authors.affiliation: Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. takeuchi@iis.u-tokyo.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: F
authors.initials: F
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007545:Isoproterenol / Q000494:pharmacology; D056656:Lab-On-A-Chip Devices; D009200:Myocardial Contraction / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D011433:Propranolol / Q000494:pharmacology; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011433:Propranolol; D007545:Isoproterenol