title: Magnesium inference screw supports early graft incorporation with inhibition of graft degradation in anterior cruciate ligament reconstruction.
abstract: Patients after anterior cruciate ligament (ACL) reconstruction surgery commonly encounters graft failure in the initial phase of rehabilitation. The inhibition of graft degradation is crucial for the successful reconstruction of the ACL. Here, we used biodegradable high-purity magnesium (HP Mg) screws in the rabbit model of ACL reconstruction with titanium (Ti) screws as a control and analyzed the graft degradation and screw corrosion using direct pull-out tests, microCT scanning, and histological and immunohistochemical staining. The most noteworthy finding was that tendon graft fixed by HP Mg screws exhibited biomechanical properties substantially superior to that by Ti screws and the relative area of collagen fiber at the tendon-bone interface was much larger in the Mg group, when severe graft degradation was identified in the histological analysis at 3 weeks. Semi-quantitative immunohistochemical results further elucidated that the MMP-13 expression significantly decreased surrounding HP Mg screws with relatively higher Collagen II expression. And HP Mg screws exhibited uniform corrosion behavior without displacement or loosening in the femoral tunnel. Therefore, our results demonstrated that Mg screw inhibited graft degradation and improved biomechanical properties of tendon graft during the early phase of graft healing and highlighted its potential in ACL reconstruction.
authors.affiliation: Department of Orthopaedic, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Orthopaedic, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Han
authors.affiliation: State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Changli
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.|Suzhou Origin Medical Technology Co. Ltd., Suzhou 215513, China.
authors.forename: Shaoxiang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.|Suzhou Origin Medical Technology Co. Ltd., Suzhou 215513, China.
authors.forename: Xiaonong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Chai
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D000070598:Anterior Cruciate Ligament Injuries / Q000378:metabolism / Q000601:surgery*; D059549:Anterior Cruciate Ligament Reconstruction / Q000295:instrumentation* / Q000379:methods; D001696:Biomechanical Phenomena; D001863:Bone Screws; D024043:Collagen Type II / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation; D006085:Graft Survival; D006801:Humans; D008274:Magnesium / Q000494:pharmacology*; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D011817:Rabbits; D014025:Titanium / Q000009:adverse effects
chemical_list: D024043:Collagen Type II; D014025:Titanium; D053509:Matrix Metalloproteinase 13; D008274:Magnesium