title: [MiR-135b promotes proliferation of endometrial carcinoma cells by targeting FOXO1].
abstract: OBJECTIVE
  To explore the expression of miR-135b in endometrial carcinoma and the mechanism by which miR-135b promotes the proliferation of endometrial cancer cells.
  
  
  METHODS
  The expressions of miR-135b and FOXO1 were using RT-PCR detected in 22 fresh endometrial cancer tissues and paired adjacent tissues and also in endometrial cancer cell lines JEC, Ishikawa, HEC-1-B, and RL-952. The RL-952 and Ishikawa cell lines were transfected with miR-135b mimics or inhibitors, and the changes in their proliferative activity were detected with MTT assay; the expressions of FOXO1 mRNA and protein were detected by RT-PCR and Western blotting, respectively.
  
  
  RESULTS
  The expression of miRNA135b was significantly up-regulated and FOXO1 expression was down-regulated in endometrial carcinoma tissues as compared with the adjacent tissues (P<0.05). The mRNA expression of miR-135b was negatively correlated with the expression of FOXO1 in endometrial carcinoma. In RL-952 and Ishikawa cell lines, transfection with miR-135b mimics obviously promoted the cell proliferation (P<0.05). Up-regulation of miR-135b significantly decreased the expressions of FOXO1 protein and mRNA (P<0.05), and down- regulation of miR-135b increased FOXO1 expressions (P<0.05).
  
  
  CONCLUSIONS
  MiR-135b plays an important role in the occurrence and development of endometrial carcinoma partially by regulating its target gene FOXO1.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical Universityl,Guangzhou 510515, China.E-mail: 1019217556@qq.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yue
authors.forename: Jun-Jing
authors.initials: JJ
authors.lastname: Shen
authors.forename: Qi-Tao
authors.initials: QT
authors.lastname: Huang
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Qin
authors.forename: Xue-Nong
authors.initials: XN
authors.lastname: Li
authors.forename: Guo-Bing
authors.initials: GB
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D014162:Transfection; D015854:Up-Regulation
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C544965:MIRN135 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger