title: Spread of Plasmids Carrying Multiple GES Variants.
abstract: Five GES-producing Enterobacteriaceae isolates that displayed an extended-spectrum β-lactamase (ESBL) phenotype harbored two GES variants: GES-7 ESBL and GES-6 carbapenemase. In all isolates, the two GES alleles were located on the same integron that was inserted into an 80-kb IncM1 self-conjugative plasmid. Whole-genome sequencing suggested in vivo horizontal gene transfer of the plasmid along with clonal diffusion of Enterobacter cloacae To our knowledge, this is the first description in Europe of clustered Enterobacteriaceae isolates carrying two GES β-lactamases, of which one has extended activity toward carbapenems.
authors.affiliation: Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicetre, France French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Cuzon
authors.affiliation: Belgian National Reference Centre for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.affiliation: Belgian National Reference Centre for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Bauraing
authors.affiliation: Belgian National Reference Centre for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
authors.forename: Te-Din
authors.initials: TD
authors.lastname: Huang
authors.affiliation: Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicetre, France French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France Evolution and Ecology of Resistance to Antibiotics (EERA) Unit, Institut Pasteur-APHP-Université Paris Sud, Le Kremlin-Bicetre, France.
authors.forename: Rémy A
authors.identifier: 0000-0002-2307-3232
authors.initials: RA
authors.lastname: Bonnin
authors.affiliation: Belgian National Reference Centre for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
authors.affiliation: Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicetre, France French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France Evolution and Ecology of Resistance to Antibiotics (EERA) Unit, Institut Pasteur-APHP-Université Paris Sud, Le Kremlin-Bicetre, France thierry.naas@bct.aphp.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D022761:Gene Transfer, Horizontal / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase