title: A novel ratiometric two-photon fluorescent probe for imaging of Pd(2+) ions in living cells and tissues.
abstract: Ratiometric two-photon fluorescent probes can not only eliminate interferences from environmental factors but also achieve deep-tissue imaging with improved spatial localization. To quantitatively track Pd(2+) in biosystems, herein, we reported a ratiometric two-photon fluorescent probe, termed as Np-Pd, which based on a D-π-A-structure two-photon fluorophore of the naphthalimide derivative and deprotection of aryl propargyl ethers by palladium species. The probe Np-Pd displayed a more than 25-fold enhancement towards palladium species with high sensitivity and selectivity. Additionally, the probe Np-Pd was further used for fluorescence imaging of Pd(2+) ions in living cells and tissues under two-photon excitation (820nm), which showed large tissue-imaging depth (19.6-184.6μm), and a high resolution for ratiometric imaging.
authors.affiliation: College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China; Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, PR China; Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China. Electronic address: zhouly0817@163.com.
authors.forename: Liyi
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China.
authors.forename: Shunqin
authors.initials: S
authors.lastname: Hu
authors.affiliation: College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China.
authors.forename: Haifei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, PR China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000032:analysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D053644:Naphthalimides / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010165:Palladium / Q000032:analysis*; D017785:Photons
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D053644:Naphthalimides; D010165:Palladium
keywords: Fluorescent imaging; Naphthalimide derivative; Palladium; Ratiometric fluorescent probe; Two-photon