title: Regulation of viral gene expression by the herpes simplex virus 1UL24 protein (HSV-1UL24 inhibits accumulation of viral transcripts).
abstract: UL24 is conserved among all Herpesviridae. In herpes simplex virus 1 (HSV-1), UL24 mutations lead to reduced viral titers both in cell culture and in vivo, and reduced pathogenicity. The human cytomegalovirus ortholog of UL24 has a gene regulatory function; however, it is not known whether other UL24 orthologs also affect gene expression. We discovered that in co-transfection experiments, expression of UL24 correlated with a reduction in the expression of several viral proteins and transcripts. Substitution mutations targeting conserved residues in UL24 impaired this function. Reduced transcript levels did not appear attributable to changes in mRNA stability. The UL24 ortholog of Herpes B virus exhibited a similar activity. An HSV-1 mutant that does not express UL24 produced more viral R1 and R2 transcripts than the wild type or rescue virus relative to the amount of viral DNA. These results reveal a new role for HSV-1UL24 in regulating viral mRNA accumulation.
authors.affiliation: INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada H7V 1B7.
authors.forename: Carolina
authors.initials: C
authors.lastname: Sanabria-Solano
authors.affiliation: INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada H7V 1B7.
authors.forename: Carmen Elena
authors.initials: CE
authors.lastname: Gonzalez
authors.affiliation: INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada H7V 1B7.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Richerioux
authors.affiliation: INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada H7V 1B7.
authors.forename: Luc
authors.initials: L
authors.lastname: Bertrand
authors.affiliation: INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada H7V 1B7.
authors.forename: Slimane
authors.initials: S
authors.lastname: Dridi
authors.affiliation: Texas Biomedical Research Institute, 7620 NW Loop 410, San Antonio, TX 78227-5301, United States.
authors.forename: Anthony
authors.initials: A
authors.lastname: Griffiths
authors.affiliation: CRCHUM (Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Pavillon R, 900 Saint-Denis, Montréal, Canada H2X 0A9.
authors.forename: Yves
authors.initials: Y
authors.lastname: Langelier
authors.affiliation: INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada H7V 1B7. Electronic address: angela.pearson@iaf.inrs.ca.
authors.forename: Angela
authors.initials: A
authors.lastname: Pearson
mesh_terms: D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors / Q000235:genetics; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D009154:Mutation; D020871:RNA Stability; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: C086267:UL24 protein, Human herpesvirus 1; D014764:Viral Proteins
keywords: Herpes simplex virus 1; UL24; Viral gene regulation