title: The impact of langerin (CD207)+ dendritic cells and FOXP3+ Treg cells in the small bowel mucosa of children with celiac disease and atopic dermatitis in comparison to children with functional gastrointestinal disorders.
abstract: In the present study we aimed to evaluate the impact of langerin (CD207)+ dendritic cells (DCs) and FOXP3+ Treg cells in the intestinal mucosa of children with celiac disease (CD) and atopic dermatitis (AD) in comparison to children with functional gastrointestinal disorders (FGD). Seventy-five children (37 male, mean age 8.4 ± 4.8 years), who randomly underwent small bowel biopsy, were studied. The CD was diagnosed in 14 children, including five persons with concomitant AD (all positive for anti-tissue transglutaminase IgA antibodies and with small bowel atrophy). Normal small bowel mucosa was found in eight patients with AD and in 53 patients with FGD. The sera of all patients were tested for total and specific IgE antibodies to food allergen panels. Staining for CD11c+, langerin (CD207+) DCs, CD4+, and FOXP3+ Treg cells was performed on paraffin-embedded sections of bioptates using immunohistochemistry. The density of CD11c+ DCs, CD4+, and FOXP3+ Treg cells was higher in the CD patients compared to the AD and FGD patients (p = 0.02; p = 0.001). In AD, significantly higher density of CD11c+ DCs was detected in patients positive for specific IgE to food allergen panels (p = 0.02). The FGD patients with elevated total IgE had increased density of langerin (CD207)+ DCs compared to the patients with normal total IgE levels (p = 0.01). The increased density of FOXP3+ Treg cells, CD4+, cells and CD11c+ DCs was associated with CD but not with AD. The elevated level of total IgE or specific IgE to food allergens was associated with more pronounced expression of DCs, indicating a possible link between the presence of these cells in small bowel mucosa with elevated level of serum IgE.
authors.affiliation: Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Vorobjova
authors.affiliation: Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.|East-Tallinn Central Hospital, Tallinn, Estonia.
authors.forename: Krista
authors.initials: K
authors.lastname: Ress
authors.affiliation: Tallinn Children's Hospital, Tallinn, Estonia.
authors.forename: Katrin
authors.initials: K
authors.lastname: Luts
authors.affiliation: Department of Pediatrics, University of Tartu, Tartu, Estonia.|Children's Clinic of Tartu University Hospital, Tartu, Estonia.
authors.forename: Oivi
authors.initials: O
authors.lastname: Uibo
authors.affiliation: Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
authors.forename: Raivo
authors.initials: R
authors.lastname: Uibo
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D001706:Biopsy; D039521:CD11c Antigen / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D002446:Celiac Disease / Q000150:complications / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000473:pathology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis; D005767:Gastrointestinal Diseases / Q000473:pathology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000473:pathology*; D007421:Intestine, Small / Q000473:pathology; D037181:Lectins, C-Type / Q000032:analysis; D008297:Male; D037241:Mannose-Binding Lectins / Q000032:analysis; D011897:Random Allocation; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry / Q000276:immunology*
chemical_list: D000485:Allergens; D015703:Antigens, CD; D039521:CD11c Antigen; C121916:CD207 protein, human; D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D007073:Immunoglobulin E
keywords: Celiac disease; T reg cells; atopic dermatitis; dendritic cells; immunohistochemistry; small bowel mucosa