title: Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories.
abstract: Sustainable production of oleochemicals requires establishment of cell factory platform strains. The yeast Saccharomyces cerevisiae is an attractive cell factory as new strains can be rapidly implemented into existing infrastructures such as bioethanol production plants. Here we show high-level production of free fatty acids (FFAs) in a yeast cell factory, and the production of alkanes and fatty alcohols from its descendants. The engineered strain produces up to 10.4 g l(-1) of FFAs, which is the highest reported titre to date. Furthermore, through screening of specific pathway enzymes, endogenous alcohol dehydrogenases and aldehyde reductases, we reconstruct efficient pathways for conversion of fatty acids to alkanes (0.8 mg l(-1)) and fatty alcohols (1.5 g l(-1)), to our knowledge the highest titres reported in S. cerevisiae. This should facilitate the construction of yeast cell factories for production of fatty acids derived products and even aldehyde-derived chemicals of high value.
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-41296, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg SE41296, Sweden.
authors.forename: Yongjin J
authors.initials: YJ
authors.lastname: Zhou
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-41296, Sweden.
authors.forename: Nicolaas A
authors.initials: NA
authors.lastname: Buijs
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-41296, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg SE41296, Sweden.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-41296, Sweden.
authors.forename: Jiufu
authors.initials: J
authors.lastname: Qin
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-41296, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg SE41296, Sweden.
authors.forename: Verena
authors.initials: V
authors.lastname: Siewers
authors.affiliation: Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-41296, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg SE41296, Sweden.|Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm DK2970, Denmark.|Science for Life Laboratory, Royal Institute of Technology, Stockholm SE-17121, Sweden.
authors.forename: Jens
authors.identifier: http://orcid.org/0000-0002-9955-6003
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D000473:Alkanes / Q000378:metabolism*; D056804:Biofuels; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis*; D005233:Fatty Alcohols / Q000378:metabolism*; D060847:Metabolic Engineering; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000473:Alkanes; D056804:Biofuels; D005230:Fatty Acids, Nonesterified; D005233:Fatty Alcohols