title: Cytokine-induced killer cells interact with tumor lysate-pulsed dendritic cells via CCR5 signaling.
abstract: The antitumor activity of cytokine-induced killer (CIK) cells can be increased by co-culturing them with tumor lysate-pulsed dendritic cells (tDCs); this phenomenon has been studied mainly at the population level. Using time-lapse imaging, we examined how CIK cells gather information from tDCs at the single-cell level. tDCs highly expressed CCL5, which bound CCR5 expressed on CIK cells. tDCs strongly induced migration of Ccr5(+/+) CIK cells, but not that of Ccr5(-/-) CIK cells or Ccr5(+/+) CIK cells treated with the CCR5 antagonist Maraviroc. Individual tDCs contacted Ccr5(+/+) CIK cells more frequently and lengthily than with Ccr5(-/-) CIK cells. Consequently, tDCs increased the antitumor activity of Ccr5(+/+) CIK cells in vitro and in vivo, but did not increase that of Ccr5(-/-) CIK cells. Taken together, our data provide insight into the mechanism of CIK cell activation by tDCs at the single-cell level.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Hong Kyung
authors.initials: HK
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Yong Guk
authors.initials: YG
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Ji Sung
authors.initials: JS
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Eun Jae
authors.initials: EJ
authors.lastname: Park
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Boyeong
authors.initials: B
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Ki Hwan
authors.initials: KH
authors.lastname: Park
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungbuk 28116, Republic of Korea.
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Kang
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea. Electronic address: shan@chungbuk.ac.kr.
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
mesh_terms: D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000276:immunology / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D018920:Coculture Techniques; D055612:Cytokine-Induced Killer Cells / Q000276:immunology / Q000378:metabolism* / Q000637:transplantation; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000637:transplantation; D005838:Genotype; D008546:Melanoma, Experimental / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D034622:RNA Interference; D019713:Receptors, CCR5 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D059008:Time-Lapse Imaging; D014162:Transfection; D047368:Tumor Burden
chemical_list: C000592874:CCR5 protein, mouse; C503460:Ccl5 protein, mouse; D018946:Chemokine CCL5; D019713:Receptors, CCR5
keywords: Cell–cell interaction; Migration; Time-lapse imaging