title: Tepidibacillus infernus sp. nov., a moderately thermophilic, selenate- and arsenate-respiring hydrolytic bacterium isolated from a gold mine, and emended description of the genus Tepidibacillus.
abstract: A novel aerotolerant anaerobic, moderately thermophilic, organotrophic bacterium, strain MBL-TLPT, was isolated from a sample of microbial mat, developed under the flow of subsurface water in TauTona gold mine, South Africa. Cells of the new isolate were flagellated, spore-forming rods, 0.25-0.5 µm in width and 3-15 µm in length. Strain MBL-TLPT grew in the temperature range from 25 to 58 °C, pH range from 5.6 to 8.8 and at NaCl concentration from 0 to 85 g l-1. The isolate was able to ferment yeast extract and mono-, oligo- and polysaccharides, including starch and xanthan gum. The G+C content of the DNA was 35 mol%. Phylogenetic analysis of 16S rRNA gene sequences of strain MBL-TLPT and relatives showed its affiliation to the genus Tepidibacillus. Tepidibacillus fermentans STGHT was its closest relative (97.1 % identity of 16S rRNA gene sequences). Based on phylogenetic analysis and the physiological properties of the novel isolate, we propose a novel species, Tepidibacillus infernus sp. nov., with MBL-TLPT(=DSM 28123T=VKM В-2949T) as the type strain.
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Podosokorskaya
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Merkel
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Gavrilov
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Igor
authors.initials: I
authors.lastname: Fedoseev
authors.affiliation: TIA-UFS SAENSE Platform, University of the Free State, Bloemfontein, South Africa.
authors.forename: Esta van
authors.initials: EV
authors.lastname: Heerden
authors.affiliation: TIA-UFS SAENSE Platform, University of the Free State, Bloemfontein, South Africa.
authors.forename: Errol D
authors.initials: ED
authors.lastname: Cason
authors.affiliation: Gubkin Russian State, University of Oil and Gas, Moscow, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Novikov
authors.affiliation: Bioengineering Center, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Kolganova
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: Aleksei A
authors.initials: AA
authors.lastname: Korzhenkov
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
authors.affiliation: Winogradsky Institute of Microbiology, Research Center for Biotechnology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Kublanov
mesh_terms: D001149:Arsenates / Q000378:metabolism; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006046:Gold; D008906:Mining; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D064586:Selenic Acid / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013019:South Africa
chemical_list: D001149:Arsenates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D006046:Gold; D064586:Selenic Acid; C025657:arsenic acid