title: Antiproliferative and Apoptotic Effect of Curcumin and TRAIL (TNF Related Apoptosis inducing Ligand) in Chronic Myeloid Leukaemic Cells.
abstract: BACKGROUND
  Curcumin, traditionally utilized as a flavouring zest as a part of Indian cooking, has been accounted to decrease the proliferation potential of most cancer cells. Apoptosis is a mechanism by which most anticancer therapies including chemotherapy, radiation and antihormonal therapy kill tumour/cancer cells. Novel agents that may sensitize drug-resistant tumour cells for induction of apoptosis by customary treatments could lead to the regression and improved prognosis of the refractory disease. Indeed, chemotherapeutic agents have been shown to sensitize cancer cells to killing by death ligands such as tumour necrosis factor-α.
  
  
  OBJECTIVE
  To investigate cytotoxicity and apoptotic effect of curcumin in chronic myeloid leukaemic cell line KCL-22.
  
  
  METHODS
  In present study, different doses of curcumin (10,25,50,75,100μM) and tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) (25,50 μM) alone and combine regimen were exposed to myeloid leukaemic cell KCL-22. The cell viability was monitored by MTT assay, apoptotic activity by binding of Annexin V-FITC using fluorescence microscopy and cell cycle check points by flow cytometry.
  
  
  RESULTS
  Cytotoxic assay revealed that curcumin and TRAIL induced both dose and time-dependent decrease in cell viability. Significant cell cytotoxicity was seen in combine regimen of both curcumin and TRAIL at 48 h of exposure. Cells treated with curcumin and TRAIL was arrested at the S phase, as revealed by flow cytometric analysis. Subtoxic concentrations of the curcumin-TRAIL combination induced strong apoptotic response in KCL-22 cells as demonstrated by the binding of Annexin V-FITC.
  
  
  CONCLUSIONS
  Our study conclude that curcumin inhibits the cancer cell growth by inducing apoptosis and enhance the therapeutic potential of TRAIL which recommends that both curcumin alone or in combination with TRAIL might be useful for leukaemic prevention and better therapeutic responses.
authors.affiliation: PhD Student, Department of Physiology, King George Medical University , Lucknow, India .
authors.forename: Bushra
authors.initials: B
authors.lastname: Iqbal
authors.affiliation: Associate Professor, Department of Physiology, King George Medical University , Lucknow, India .
authors.forename: Archna
authors.initials: A
authors.lastname: Ghildiyal
authors.affiliation: PhD Student, Department of Zoology, Lucknow University , India .
authors.lastname: Sahabjada
authors.affiliation: Professor, Department of Physiology, King George Medical University , Lucknow, India .
authors.forename: Shraddha
authors.initials: S
authors.lastname: Singh
authors.affiliation: Professor, Department of Zoology, Lucknow University , India .
authors.forename: Mohd
authors.initials: M
authors.lastname: Arshad
authors.affiliation: Professor and Head, Department of Biochemistry, King George Medical University , Lucknow, India .
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Mahdi
authors.affiliation: Professor and Head, Department of Physiology, King George Medical University , Lucknow, India .
authors.forename: Sunita
authors.initials: S
authors.lastname: Tiwari
keywords: Apoptosis; CML; Cell cycle