title: Arabidopsis NATA1 Acetylates Putrescine and Decreases Defense-Related Hydrogen Peroxide Accumulation.
abstract: Biosynthesis of the polyamines putrescine, spermidine, and spermine is induced in response to pathogen infection of plants. Putrescine, which is produced from Arg, serves as a metabolic precursor for longer polyamines, including spermidine and spermine. Polyamine acetylation, which has important regulatory functions in mammalian cells, has been observed in several plant species. Here we show that Arabidopsis (Arabidopsis thaliana) N-ACETYLTRANSFERASE ACTIVITY1 (NATA1) catalyzes acetylation of putrescine to N-acetylputrescine and thereby competes with spermidine synthase for a common substrate. NATA1 expression is strongly induced by the plant defense signaling molecule jasmonic acid and coronatine, an effector molecule produced by DC3000, a Pseudomonas syringae strain that initiates a virulent infection in Arabidopsis ecotype Columbia-0. DC3000 growth is reduced in nata1 mutant Arabidopsis, suggesting a role for NATA1-mediated putrescine acetylation in suppressing antimicrobial defenses. During infection by P. syringae and other plant pathogens, polyamine oxidases use spermidine and spermine as substrates for the production of defense-related H2O2 Compared to wild-type Columbia-0 Arabidopsis, the response of nata1mutants to P. syringae infection includes reduced accumulation of acetylputrescine, greater abundance of nonacetylated polyamines, elevated H2O2 production by polyamine oxidases, and higher expression of genes related to pathogen defense. Together, these results are consistent with a model whereby P. syringae growth is improved in a targeted manner through coronatine-induced putrescine acetylation by NATA1.
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York 14853 (Y.-R.L., M.B., J.Y., A.S.P., G.J.); and Key Laboratory of Tropical Agro Environment, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642, PR China (J.Y.).
authors.forename: Yann-Ru
authors.identifier: 0000-0002-4716-4323
authors.initials: YR
authors.lastname: Lou
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York 14853 (Y.-R.L., M.B., J.Y., A.S.P., G.J.); and Key Laboratory of Tropical Agro Environment, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642, PR China (J.Y.).
authors.forename: Melike
authors.identifier: 0000-0002-0170-2800
authors.initials: M
authors.lastname: Bor
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York 14853 (Y.-R.L., M.B., J.Y., A.S.P., G.J.); and Key Laboratory of Tropical Agro Environment, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642, PR China (J.Y.).
authors.forename: Jian
authors.identifier: 0000-0001-8597-9841
authors.initials: J
authors.lastname: Yan
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York 14853 (Y.-R.L., M.B., J.Y., A.S.P., G.J.); and Key Laboratory of Tropical Agro Environment, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642, PR China (J.Y.).
authors.forename: Aileen S
authors.initials: AS
authors.lastname: Preuss
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York 14853 (Y.-R.L., M.B., J.Y., A.S.P., G.J.); and Key Laboratory of Tropical Agro Environment, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642, PR China (J.Y.) gj32@cornell.edu.
authors.forename: Georg
authors.identifier: 0000-0002-9675-934X
authors.initials: G
authors.lastname: Jander
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007192:Indenes / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D044224:Pseudomonas syringae / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D011700:Putrescine / Q000031:analogs & derivatives / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; D007192:Indenes; D010937:Plant Growth Regulators; D012333:RNA, Messenger; C026212:N-acetylputrescine; C058487:coronatine; D006861:Hydrogen Peroxide; D000123:Acetyltransferases; C000622659:NATA1 protein, Arabidopsis; D011700:Putrescine