title: Derivatization reaction-based surface-enhanced Raman scattering (SERS) for detection of trace acetone.
abstract: A facile method was developed for determination of trace volatile acetone by coupling a derivatization reaction to surface-enhanced Raman scattering (SERS). With iodide modified Ag nanoparticles (Ag IMNPs) as the SERS substrate, acetone without obvious Raman signal could be converted to SERS-sensitive species via a chemical derivatization reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH). In addition, acetone can be effectively separated from liquid phase with a purge-sampling device and then any serious interference from sample matrices can be significantly reduced. The optimal conditions for the derivatization reaction and the SERS analysis were investigated in detail, and the selectivity and reproducibility of this method were also evaluated. Under the optimal conditions, the limit of detection (LOD) for acetone was 5mgL(-1) or 0.09mM (3σ). The relative standard deviation (RSD) for 80mgL(-1) acetone (n=9) was 1.7%. This method was successfully used for the determination of acetone in artificial urine and human urine samples with spiked recoveries ranging from 92% to 110%. The present method is convenient, sensitive, selective, reliable and suitable for analysis of trace acetone, and it could have a promising clinical application in early diabetes diagnosis.
authors.affiliation: Key Laboratory of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Key Laboratory of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, Changwon National University, Changwan 641773, Republic of Korea.
authors.forename: Yong-Ill
authors.initials: YI
authors.lastname: Lee
authors.affiliation: Key Laboratory of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China; Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Xiandeng
authors.initials: X
authors.lastname: Hou
authors.affiliation: Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan 610064, China. Electronic address: wuli@scu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.affiliation: Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan 610064, China. Electronic address: tianfay@sohu.com.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000096:Acetone / Q000032:analysis* / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010659:Phenylhydrazines / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D010659:Phenylhydrazines; D000096:Acetone; C004787:2,4-dinitrophenylhydrazine; D012834:Silver
keywords: Acetone; Ag IMNPs; Derivatization reaction; Surface-enhanced Raman scattering; Urine