title: Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea.
abstract: A novel allele-specific Rfo marker was developed and proved to be effective for MAS of Rfo gene in B. oleracea background and six Ogu-CMS fertility-restored interspecific hybrids were created for the first time. Ogura cytoplasmic male sterility (Ogu-CMS) has been extensively used for Brassica oleracea hybrid production. However, because of maternal inheritance, all the hybrids produced by CMS lines are male sterile and cannot be self-pollinated, which prohibits germplasm maintenance and innovation. This problem can be overcome by using the Ogu-CMS restorer line, but restorer material is absent in B. oleracea crops. Here, Rfo, a fertility-restored gene of Ogu-CMS, was transferred from rapeseed restorer lines into a Chinese kale Ogu-CMS line using interspecific hybridization combined with embryo rescue. Nine interspecific, triploid plant progenies were identified at morphological and ploidy level, with phenotypes intermediate between those of rapeseed and Chinese kale. Because the Rfo marker (Hu et al., Mol Breeding 22:663-674, 2008) cannot distinguish the Rfo and its homologies under a B. oleracea background, a novel allele-specific Rfo marker was developed based on the BLAST analysis of highly homologous Rfo sequences in B. oleracea. Screening using the novel Rfo marker found that six interspecific hybrids carrying Rfo were also fertile, although fertility varied during different flowering periods. Furthermore, BC1 offsprings with the Rfo gene were selected with the allele-specific Rfo marker and showed restored fertility. These results indicated that the novel allele-specific marker could be used for the MAS of Rfo gene in B. oleracea, and this study lays the foundation for the development of Ogu-CMS restorer material in cabbage and its related other subspecies.
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Yu
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Fang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Yang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Mu
authors.initials: M
authors.lastname: Zhuang
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Lv
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Zhan-Sheng
authors.initials: ZS
authors.lastname: Li
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Feng-Qing
authors.initials: FQ
authors.lastname: Han
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Liu
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, People's Republic of China. zhangyangyong@caas.cn.
authors.forename: Yang-Yong
authors.initials: YY
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001937:Brassica / Q000235:genetics* / Q000502:physiology; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D006824:Hybridization, Genetic; D051479:Plant Infertility / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers