title: [Nickel smelting dust exposures to NIH/3T3 cellular mitochondrial damage and L-ascorbic acid interference effect].
abstract: OBJECTIVE
  To study the protection of L-ascorbic acid on mouse embryonic fibroblasts (NIH/3T3) from carcinogenic effects caused by nickel smelting smoke subjects.
  
  
  METHODS
  The NIH/3T3 cells were divided into experimental and L-ascorbic acid in the intervention group. Plus exposure group concentration of nickel refining dusts were formulate 0.00, 25.00, 50.00, 100.00 μg/ml suspension, the intervention group on the basis of the added exposure group containing L-ascorbic acid (100 mmol/L) , contacted. Then, the cell viability was detected by MTT assay, we used Calcein-AM fluorescence probe to detect cell mitochondrial permeability transition pore (MPTP) changes, JC-1 staining to observe and detect the cell mitochondrial membrane potential (MMP) change, colorimetric quantitative to study the activity of mitochondrial respiratory chain complex Ⅰ,Ⅱ,Ⅲ,Ⅳ.
  
  
  RESULTS
  Upon 24 h incubation, both cell relative inhibition rate, openness of MPTP were increasing enhanced by different concentrations, on the other hand, MMP and the activity of mitochondrial respiratory chain complexⅠ, Ⅱ, Ⅳ were obviously decreased, the differences were statistically significant (P<0.05) .After L-ascorbic acid intervention treatment, the results of the intervention group were lower than that of the exposure group, and the difference was statistically significant (P<0.05) , indicating the protection of L-ascorbic acid on cell mitochondrial from the nickel exposure damage.
  
  
  CONCLUSIONS
  The damage effects of nickel on NIH/3T3 cell mitochondrial was significantly enhanced with the increasing concentration, and L-ascorbic acid has certain protection on cellular mitochondrial.
authors.affiliation: School of Public Heath, Harbin Medical University, Harbin 150086, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Pan
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.forename: S Y
authors.initials: SY
authors.lastname: Wang
authors.forename: W Q
authors.initials: WQ
authors.lastname: Tan
authors.forename: J S
authors.initials: JS
authors.lastname: Chen
authors.forename: Y H
authors.initials: YH
authors.lastname: Wu
mesh_terms: D000818:Animals; D001205:Ascorbic Acid; D002470:Cell Survival; D004391:Dust; D005452:Fluoresceins; D051379:Mice; D008928:Mitochondria; D041681:NIH 3T3 Cells; D009532:Nickel
chemical_list: D004391:Dust; D005452:Fluoresceins; C085925:calcein AM; D009532:Nickel; D001205:Ascorbic Acid