title: Isolation and detection of microRNA from the egg of chickens.
abstract: BACKGROUND
  The egg is a vital part of the chicken developmental process and an important protein source for humans. Despite the chicken egg being a subject of intense research little attention has been given to the role of microRNAs within the egg.
  
  
  RESULTS
  We report a method for the reproducible and reliable isolation of miRNA from the albumen and yolk of chicken eggs. We also report the detection via real-time PCR of a number of miRNAs from both of these biological fluids.
  
  
  CONCLUSIONS
  These findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg. This method of detecting miRNAs in chicken eggs will allow researchers to investigate the presence of an additional level of epigenetic programming in chick development previously unknown and also how this impacts the nutritional value of eggs for human consumption.
authors.affiliation: School of Medicine, MMR, Bioinformatics Core Research Facility, Deakin University, Pigdons Road, Waurn Ponds, VIC, 3216, Australia.|CSIRO, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC, 3220, Australia.|Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia.
authors.forename: Ben
authors.initials: B
authors.lastname: Wade
authors.affiliation: School of Medicine, MMR, Bioinformatics Core Research Facility, Deakin University, Pigdons Road, Waurn Ponds, VIC, 3216, Australia.|CSIRO, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC, 3220, Australia.|Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Cummins
authors.affiliation: CSIRO, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC, 3220, Australia.|Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia.
authors.forename: Anthony
authors.initials: A
authors.lastname: Keyburn
authors.affiliation: School of Medicine, MMR, Bioinformatics Core Research Facility, Deakin University, Pigdons Road, Waurn Ponds, VIC, 3216, Australia. tamsyn.crowley@deakin.edu.au.|CSIRO, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC, 3220, Australia. tamsyn.crowley@deakin.edu.au.|Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia. tamsyn.crowley@deakin.edu.au.
authors.forename: Tamsyn M
authors.initials: TM
authors.lastname: Crowley
mesh_terms: D000818:Animals; D002645:Chickens; D004531:Eggs; D035683:MicroRNAs / Q000032:analysis / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs
keywords: Albumen; Chicken; Egg; Extraction; Yolk; microRNA