title: Up-regulation of Wip1 involves in neuroinflammation of retinal astrocytes after optic nerve crush via NF-κB signaling pathway.
abstract: OBJECTIVE
  To evaluate the expression and possible roles of Wip1 in retinal astrocytes after optic nerve crush (ONC).
  
  
  METHODS
  Expressions of Wip1, GFAP, and p-p65 in ONC model were analyzed by Western blot and immunofluorescence. The mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were analyzed by RT-PCR.
  
  
  RESULTS
  Wip1 was up-regulated at 14 days after ONC by Western blot and immunofluorescence. The changes of Wip1 were striking in astrocytes. Furthermore, the protein expression level of p-p65 was paralleled with Wip1 in a time-dependent manner by ONC. In addition, co-localization of Wip1 with Phospho-NF-κB-p65 (p-p65) was detected. Finally, the mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were significantly increased in retina after ONC.
  
  
  CONCLUSIONS
  These data were consistent with the hypothesis that Wip1 was implicated in neuroinflammation of retinal astrocytes after ONC via NF-κB signaling pathway.
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Zhong
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Lifei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China. eyezsm@163.com.
authors.forename: Siming
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China. eyeminli@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D016207:Cytokines / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D020221:Optic Nerve Injuries / Q000378:metabolism*; D000071636:Protein Phosphatase 2C / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D016207:Cytokines; C000614142:Gfap protein, rat; D005904:Glial Fibrillary Acidic Protein; D016328:NF-kappa B; D000071636:Protein Phosphatase 2C
keywords: Astrocytes; NF-κB; Neuroinflammation; Optic nerve crush; Wip1