title: Evaluation of99mTc-resveratrol as a colon cancer targeting probe.
abstract: The study aimed to evaluate cancer-targeting potential of a newly synthesised radiopharmaceutical,99mTc-resveratrol in vivo, using colon cancer model. Colon cancer was induced in 20 male Sprague-Dawley rats by subcutaneous administration of 1,2-dimethylhydrazine (DMH), dissolved in 1 mM EDTA-normal saline, at a dose of 30 mg/kg body weight twice a week for first 4 weeks and once a week for next 12 weeks. A control group containing normal rats was used for result comparison. Colon cancer in DMH-treated group was confirmed by gross analysis of the colon, by histopathological analysis and molecular marker study in tumour tissue. At the end of the treatment period, the animals from the two groups were used for bio-distribution evaluation of99mTc-resveratrol at different time intervals. High uptake of99mTc-resveratrol was recorded in rat liver, spleen and kidneys, and the ratio of colon tumour uptake to normal colon uptake in DMH-treated rats increased significantly (P ≤ 0.01) with time, to reach a maximum value at 2 h but decreased thereafter. High uptake at the tumour site as compared to normal colon tissue was observed; however, the uptake by cancer cells at the target site was limited by high reticulo-endothelial uptake and rapid metabolism.
authors.affiliation: Centre for Nuclear Medicine, University Institute of Emerging Areas in Science and Technology (UIEAST), Panjab University, Chandigarh, India.
authors.forename: R
authors.initials: R
authors.lastname: Kamal
authors.affiliation: Department of Biophysics, Panjab University, Chandigarh, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Dhawan
authors.affiliation: Centre for Nuclear Medicine, University Institute of Emerging Areas in Science and Technology (UIEAST), Panjab University, Chandigarh, India.
authors.forename: V D
authors.initials: VD
authors.lastname: Chadha
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000000981:diagnostic imaging*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000000981:diagnostic imaging*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013154:Spleen / Q000378:metabolism; D013267:Stilbenes / Q000493:pharmacokinetics*; D013667:Technetium / Q000493:pharmacokinetics*
chemical_list: D002273:Carcinogens; D019275:Radiopharmaceuticals; D013267:Stilbenes; D013667:Technetium; D019813:1,2-Dimethylhydrazine; D000077185:Resveratrol
keywords: colon cancer; radiolabel; resveratrol