title: Denaturation and in Vitro Gastric Digestion of Heat-Treated Quinoa Protein Isolates Obtained at Various Extraction pH.
abstract: The aim of this study was to determine the influence of heat processing on denaturation and digestibility properties of protein isolates obtained from sweet quinoa (Chenopodium quinoaWilld) at various extraction pH values (8, 9, 10 and 11). Pretreatment of suspensions of protein isolates at 60, 90 and 120 °C for 30 min led to protein denaturation and aggregation, which was enhanced at higher treatment temperatures. The in vitro gastric digestibility measured during 6 h was lower for protein extracts pre-treated at 90 and 120 °C compared to 60 °C. The digestibility decreased with increasing extraction pH, which could be ascribed to protein aggregation. Protein digestibility of the quinoa protein isolates was higher compared to wholemeal quinoa flour. We conclude that an interactive effect of processing temperature and extraction pH on in vitro gastric digestibility of quinoa protein isolates obtained at various extraction pH is observed. This gives a first indication of how the nutritional value of quinoa protein could be influenced by heat processing, protein extraction conditions and other grain components.
authors.affiliation: Food and Biobased Research, Wageningen University and Research Centre, P.O. Boxs 17, 6700 AA Wageningen, Netherlands ; Food Quality and Design Group, Wageningen University and Research Centre, P.O. Boxs 17, 6700 AA Wageningen, Netherlands.
authors.forename: Geraldine Avila
authors.initials: GA
authors.lastname: Ruiz
authors.affiliation: Food Process Engineering, Wageningen University and Research Centre, P.O. Box 17, 6700 AA Wageningen, Netherlands.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Opazo-Navarrete
authors.affiliation: Food Quality and Design Group, Wageningen University and Research Centre, P.O. Boxs 17, 6700 AA Wageningen, Netherlands.
authors.forename: Marlon
authors.initials: M
authors.lastname: Meurs
authors.affiliation: Food and Biobased Research, Wageningen University and Research Centre, P.O. Boxs 17, 6700 AA Wageningen, Netherlands.
authors.forename: Marcel
authors.initials: M
authors.lastname: Minor
authors.affiliation: Food and Biobased Research, Wageningen University and Research Centre, P.O. Boxs 17, 6700 AA Wageningen, Netherlands.
authors.forename: Guido
authors.initials: G
authors.lastname: Sala
authors.affiliation: Food Quality and Design Group, Wageningen University and Research Centre, P.O. Boxs 17, 6700 AA Wageningen, Netherlands.
authors.forename: Martinus
authors.initials: M
authors.lastname: van Boekel
authors.affiliation: Division of Human Nutrition, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, Netherlands.
authors.forename: Markus
authors.initials: M
authors.lastname: Stieger
authors.affiliation: Food Process Engineering, Wageningen University and Research Centre, P.O. Box 17, 6700 AA Wageningen, Netherlands.
authors.forename: Anja E M
authors.initials: AE
authors.lastname: Janssen
keywords: Denaturation; Digestibility; Extraction pH; Heat processing; Protein; Quinoa