title: Analyzing Spindle Positioning Dynamics in Cultured Cells.
abstract: As spindle positioning during mitosis is a highly dynamic process, live cell imaging is a key technology when studying its underlying mechanisms. Recent advances in imaging tools and microscope systems have enabled us to simultaneously visualize several cellular components in living cells with high temporal and spatial resolution. By combining live cell imaging with functional assays such as RNAi-based depletions, drug inhibition, and micropatterned coverslips, novel and unexpected mechanisms of spindle positioning have been uncovered. In this chapter, I present methods for analyzing the dynamics of spindle positioning in cultured cells.
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Room 227 Building A, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan. kiyomitsu@bio.nagoya-u.ac.jp.|Precursory Research for Embryonic Science and Technology (PRESTO) Program, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan. kiyomitsu@bio.nagoya-u.ac.jp.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kiyomitsu
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002877:Chromosomes, Human; D015870:Gene Expression; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D008677:Metaphase / Q000235:genetics; D008856:Microscopy, Fluorescence; D008938:Mitosis; D034622:RNA Interference; D008941:Spindle Apparatus / Q000378:metabolism*
keywords: Cortical dynein; Live cell imaging; Membrane elongation; Spindle oscillation; Spindle positioning