title: The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus.
abstract: The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.
authors.affiliation: Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: Jordi F
authors.initials: JF
authors.lastname: Pelkmans
authors.affiliation: Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: Aurin M
authors.initials: AM
authors.lastname: Vos
authors.affiliation: Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.|Plant Breeding, Wageningen University and Research Centre, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Karin
authors.initials: K
authors.lastname: Scholtmeijer
authors.affiliation: Plant Breeding, Wageningen University and Research Centre, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Ed
authors.initials: E
authors.lastname: Hendrix
authors.affiliation: Plant Breeding, Wageningen University and Research Centre, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Johan J P
authors.initials: JJ
authors.lastname: Baars
authors.affiliation: Delft Bioinformatics Lab, Delft University of Technology, Mekelweg 4, 2628 CD, Delft, The Netherlands.
authors.forename: Thies
authors.initials: T
authors.lastname: Gehrmann
authors.affiliation: Delft Bioinformatics Lab, Delft University of Technology, Mekelweg 4, 2628 CD, Delft, The Netherlands.
authors.forename: Marcel J T
authors.initials: MJ
authors.lastname: Reinders
authors.affiliation: Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Lugones
authors.affiliation: Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands. h.a.b.wosten@uu.nl.
authors.forename: Han A B
authors.initials: HA
authors.lastname: Wösten
mesh_terms: D000364:Agaricus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D000070580:CYS2-HIS2 Zinc Fingers / Q000235:genetics* / Q000502:physiology; D048690:Fruiting Bodies, Fungal / Q000235:genetics* / Q000502:physiology*; D005786:Gene Expression Regulation; D016681:Genome, Fungal / Q000235:genetics; D012567:Schizophyllum / Q000502:physiology
keywords: Agaricus bisporus; Basidiomycete; Cys2His2; Fungi; Mushroom; Transcription factor