title: A Single Amino-Acid Substitution in the Sodium Transporter HKT1 Associated with Plant Salt Tolerance.
abstract: A crucial prerequisite for plant growth and survival is the maintenance of potassium uptake, especially when high sodium surrounds the root zone. The Arabidopsis HIGH-AFFINITY K(+) TRANSPORTER1 (HKT1), and its homologs in other salt-sensitive dicots, contributes to salinity tolerance by removing Na(+) from the transpiration stream. However, TsHKT1;2, one of three HKT1 copies in Thellungiella salsuginea, a halophytic Arabidopsis relative, acts as a K(+) transporter in the presence of Na(+) in yeast (Saccharomyces cerevisiae). Amino-acid sequence comparisons indicated differences between TsHKT1;2 and most other published HKT1 sequences with respect to an Asp residue (D207) in the second pore-loop domain. Two additional T salsuginea and most other HKT1 sequences contain Asn (n) in this position. Wild-type TsHKT1;2 and altered AtHKT1 (AtHKT1(N-D)) complemented K(+)-uptake deficiency of yeast cells. Mutant hkt1-1 plants complemented with both AtHKT1(N) (-) (D) and TsHKT1;2 showed higher tolerance to salt stress than lines complemented by the wild-type AtHKT1 Electrophysiological analysis in Xenopus laevis oocytes confirmed the functional properties of these transporters and the differential selectivity for Na(+) and K(+) based on the n/d variance in the pore region. This change also dictated inward-rectification for Na(+) transport. Thus, the introduction of Asp, replacing Asn, in HKT1-type transporters established altered cation selectivity and uptake dynamics. We describe one way, based on a single change in a crucial protein that enabled some crucifer species to acquire improved salt tolerance, which over evolutionary time may have resulted in further changes that ultimately facilitated colonization of saline habitats.
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Akhtar
authors.initials: A
authors.lastname: Ali
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Natalia
authors.identifier: 0000-0002-1801-4131
authors.initials: N
authors.lastname: Raddatz
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Rashid
authors.initials: R
authors.lastname: Aman
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Songmi
authors.identifier: 0000-0002-9667-4055
authors.initials: S
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Hyeong Cheol
authors.initials: HC
authors.lastname: Park
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Masood
authors.initials: M
authors.lastname: Jan
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Dongwon
authors.initials: D
authors.lastname: Baek
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Irfan Ullah
authors.initials: IU
authors.lastname: Khan
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Dong-Ha
authors.initials: DH
authors.lastname: Oh
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Ray A
authors.initials: RA
authors.lastname: Bressan
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Keun Woo
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Albino
authors.initials: A
authors.lastname: Maggio
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Jose M
authors.identifier: 0000-0003-4510-8624
authors.initials: JM
authors.lastname: Pardo
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.).
authors.forename: Hans J
authors.identifier: 0000-0003-3749-4959
authors.initials: HJ
authors.lastname: Bohnert
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Republic of Korea (A.A., R.A., S.K., M.J., D.B., I.U.K., S.Y.L., K.W.L., H.J.B., D.-J.Y.); Plant Biophysics, Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M-40, km 37.7, E-28223 Pozuelo de Alarcón Madrid (N.R.);Division of Ecological Adaptation Research, National Institute of Ecology (NIE), Seocheon 325-813, Republic of Korea (H.C.P.); Department of Biology, Louisiana State University, Baton Rouge, Louisiana 70803 (D.-H.O.);Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-2010 (R.A.B.);Department of Agriculture, University of Naples Federico II, Via Universita` 100, Portici, I-80055, Italy (A.M.);Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Cientificas, 41092 Sevilla, Spain (J.M.P.); College of Science, King Abdulaziz University, Jeddah 21589, KSA (H.J.B.); and Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801 (H.J.B.) djyun@gnu.ac.kr.
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D017360:Arabidopsis / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019607:Brassicaceae / Q000235:genetics; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002412:Cations / Q000378:metabolism; D005260:Female; D008958:Models, Molecular; D009865:Oocytes; D030821:Plants, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000235:genetics; D055049:Salt Tolerance / Q000502:physiology*; D027981:Symporters / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; D002412:Cations; C490286:HKT1 protein, Arabidopsis; D027981:Symporters