title: Analysis of the complementarity determining regions β-chain genomic rearrangement using high-throughput sequencing in periphery cytotoxic T lymphocytes of patients with chronic hepatitis B.
abstract: Cytotoxic T lymphocytes (CTLs) are important for the recognition of the hepatitis B virus (HBV), mediating immunoprotective mechanisms and determining the clinical outcome following HBV infection. CTLs recognize the invading virus via the T cell receptor (TCR). The aim of the current study was to investigate the variability of TCR in lymphocytes from patients with chronic hepatitis B and whether TCR genomic recombination is regulated by the current treatment strategies. Peripheral blood mononuclear cells (PBMCs) were isolated from patients with chronic hepatitis B and high‑throughput sequencing was performed to analyze the gene expression diversity of β chain complementarity determining region. High‑throughput sequencing produced ~380,000 reads. The sequences of V and J family mRNAs of the β chain V area were analyzed and databases were created for all 30 V family and J family genes. Using the Basic Local Alignment Search Tool, 15 genes were identified to be upregulated in the samples following treatment. Among them, the expression of T cell receptor β variable 28 (TRBV28)_T cell receptor β joining 1‑5 (TRBJ1.5) and TRBV6_TRBJ2.10 were significantly different in the treated samples compared with samples taken prior to treatment. Genomic recombination patterns of TRBV and TRBJ of the β chain V area were observed to be different in the samples following treatment. The data of the current study demonstrated that the genomic rearrangement of the V and J segments of TCR β chain V area may be associated with the chronic progression of HBV and impact on treatment efficacy.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Yinuo
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Shutang
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Neurology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Luo
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Physiology, University of Toronto, Toronto, ON M5S1A8, Canada.
authors.forename: Junhui
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Henan University, Kaifeng, Henan 475001, P.R. China.
authors.forename: Dazheng
authors.initials: D
authors.lastname: Han
authors.affiliation: Cancer Biotherapy Ward, Beijing You'an Hospital, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
mesh_terms: D022801:Complementarity Determining Regions / Q000235:genetics*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006515:Hepatitis B virus / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000276:immunology / Q000821:virology; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*
chemical_list: D022801:Complementarity Determining Regions