title: Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare.
abstract: Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T=JCM 30622T).
authors.affiliation: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, and Liver Research Institute, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, and Liver Research Institute, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Byoung-Jun
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, and Liver Research Institute, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hong
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, and Liver Research Institute, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Yu-Seop
authors.initials: YS
authors.lastname: Won
authors.affiliation: School of Biological Sciences, Chung-Ang University, Seoul, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Republic of Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Republic of Korea.
authors.forename: Seon Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Republic of Korea.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Lim
authors.affiliation: Korean Institute of Tuberculosis, Chungbuk, Republic of Korea.
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Korean Institute of Tuberculosis, Chungbuk, Republic of Korea.
authors.forename: Chang Ki
authors.initials: CK
authors.lastname: Kim
authors.affiliation: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, and Liver Research Institute, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
authors.affiliation: Department of Biomedical Sciences, Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, and Liver Research Institute, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D009164:Mycobacterium Infections / Q000382:microbiology; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009171:Mycolic Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S