title: Ultrasonic-assisted extraction, structure and antitumor activity of polysaccharide from Polygonum multiflorum.
abstract: Polygonum multiflorum is a popular Chinese herbal medicine with various pharmacological functions. In this study, the ultrasonic-assisted extraction condition, structural characterization and antitumor activity of a polysaccharide from roots of P. multiflorum were investigated. The ultrasonic-assisted extraction condition was optimized by single-factor experiments and response surface methodology. Results showed that the maximum extraction yield (5.49%) was obtained at ultrasonic power 158W, extraction temperature 62°C, extraction time 80min and ratio of water to material 20mL/g. The obtained crude polysaccharides were further purified to afford a neutral and an acidic fraction. The structure of the main neutral polysaccharide (named PPS with molecular weight of 3.26×10(5)Da) was characterized as a linear (1→6)-α-d-glucan by gas chromatography, Fourier transform-infrared spectroscopy, methylation analysis, 1D and 2D nuclear magnetic resonance. At the concentration of 400μg/mL, the inhibitory ratios of PPS on HepG-2 and BGC-823 cells were 53.35% and 38.58%, respectively. Results suggested this polysaccharide could be a potential natural antitumor agent.
authors.affiliation: Department of Blood Transfusion, Subei People's Hospital of Jiangsu Province, Yangzhou, 225001 Jiangsu, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Blood Transfusion, Subei People's Hospital of Jiangsu Province, Yangzhou, 225001 Jiangsu, China. Electronic address: xuexpyz@tom.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xue
authors.affiliation: College of Biological and Chemical Engineering, Yangzhou Vocational University, Yangzhou, 225009 Jiangsu, China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D009005:Monosaccharides / Q000032:analysis; D019664:Polygonaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013696:Temperature; D000069453:Ultrasonic Waves; D014867:Water / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D009005:Monosaccharides; D011134:Polysaccharides; D014867:Water
keywords: Polygonum multiflorum; Polysaccharides; Structural characterization; Ultrasound-assisted extraction