title: Co-selection of antibiotic and metal(loid) resistance in gram-negative epiphytic bacteria from contaminated salt marshes.
abstract: The goal of this study was to investigate co-selection of antibiotic resistance in gram-negative epiphytic bacteria. Halimione portulacoides samples were collected from metal(loid)-contaminated and non-contaminated salt marshes. Bacterial isolates (n=137) affiliated with Vibrio, Pseudomonas, Shewanella, Comamonas, Aeromonas and with Enterobacteriaceae. Vibrio isolates were more frequent in control site while Pseudomonas was common in contaminated sites. Metal(loid) and antibiotic resistance phenotypes varied significantly according to site contamination, and multiresistance was more frequent in contaminated sites. However, differences among sites were not observed in terms of prevalence or diversity of acquired antibiotic resistance genes, integrons and plasmids. Gene merA, encoding mercury resistance, was only detected in isolates from contaminated sites, most of which were multiresistant to antibiotics. Results indicate that metal(loid) contamination selects for antibiotic resistance in plant surfaces. In salt marshes, antibiotic resistance may be subsequently transferred to other environmental compartments, such as estuarine water or animals, with potential human health risks.
authors.affiliation: Biology Department, CESAM and iBiMED, University of Aveiro, Aveiro, Portugal. Electronic address: ihenriques@ua.pt.
authors.forename: Isabel
authors.initials: I
authors.lastname: Henriques
authors.affiliation: Biology Department, CESAM and iBiMED, University of Aveiro, Aveiro, Portugal.
authors.forename: Marta
authors.initials: M
authors.lastname: Tacão
authors.affiliation: Biology Department, CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Laura
authors.initials: L
authors.lastname: Leite
authors.affiliation: Biology Department, CESAM and iBiMED, University of Aveiro, Aveiro, Portugal.
authors.forename: Cátia
authors.initials: C
authors.lastname: Fidalgo
authors.affiliation: Biology Department, CESAM and iBiMED, University of Aveiro, Aveiro, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Araújo
authors.affiliation: Biology Department, CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Oliveira
authors.affiliation: Biology Department, CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Artur
authors.initials: A
authors.lastname: Alves
mesh_terms: D000900:Anti-Bacterial Agents; D001419:Bacteria / Q000187:drug effects*; D024881:Drug Resistance, Bacterial; D032023:Integrons; D008670:Metals; D008826:Microbial Sensitivity Tests; D053833:Wetlands
chemical_list: D000900:Anti-Bacterial Agents; D008670:Metals
keywords: Antibiotic resistance genes; Halimione portulacoides; Mobile genetic elements; Plant surface; merA gene