title: Infused polymers for cell sheet release.
abstract: Tissue engineering using whole, intact cell sheets has shown promise in many cell-based therapies. However, current systems for the growth and release of these sheets can be expensive to purchase or difficult to fabricate, hindering their widespread use. Here, we describe a new approach to cell sheet release surfaces based on silicone oil-infused polydimethylsiloxane. By coating the surfaces with a layer of fibronectin (FN), we were able to grow mesenchymal stem cells to densities comparable to those of tissue culture polystyrene controls (TCPS). Simple introduction of oil underneath an edge of the sheet caused it to separate from the substrate. Characterization of sheets post-transfer showed that they retain their FN layer and morphology, remain highly viable, and are able to grow and proliferate normally after transfer. We expect that this method of cell sheet growth and detachment may be useful for low-cost, flexible, and customizable production of cellular layers for tissue engineering.
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.|John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Juthani
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.|John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, United States.|Department of Chemical and Biological Engineering, University of Maine, 5737 Jenness Hall, Orono, ME 04469, United States.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Howell
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Haylea
authors.initials: H
authors.lastname: Ledoux
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Irini
authors.initials: I
authors.lastname: Sotiri
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.|John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Susan
authors.initials: S
authors.lastname: Kelso
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.|John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Yevgen
authors.initials: Y
authors.lastname: Kovalenko
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.|John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Amanda
authors.initials: A
authors.lastname: Tajik
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Thy L
authors.initials: TL
authors.lastname: Vu
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.|John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Lin
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Amy
authors.initials: A
authors.lastname: Sutton
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 60 Oxford Street, Cambridge, Massachusetts 02138, United States.|John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, United States.|Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.|Kavli Institute for Bionano Science and Technology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, United States.
authors.forename: Joanna
authors.initials: J
authors.lastname: Aizenberg
mesh_terms: D000818:Animals; D002460:Cell Line; D005353:Fibronectins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D009821:Oils; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D005353:Fibronectins; D009821:Oils