title: Effects of High-Temperature-Pressure Polymerized Resin-Infiltrated Ceramic Networks on Oral Stem Cells.
abstract: The development of CAD-CAM techniques called for new materials suited to this technique and offering a safe and sustainable clinical implementation. The infiltration of resin in a ceramic network under high pressure and high temperature defines a new class of hybrid materials, namely polymer infiltrated ceramics network (PICN), for this purpose which requires to be evaluated biologically. We used oral stem cells (gingival and pulpal) as an in vitro experimental model.
  
  
  
  Four biomaterials were grinded, immersed in a culture medium and deposed on stem cells from dental pulp (DPSC) and gingiva (GSC): Enamic (VITA®), Experimental Hybrid Material (EHM), EHM with initiator (EHMi) and polymerized Z100™ composite material (3M®). After 7 days of incubation; viability, apoptosis, proliferation, cytoskeleton, inflammatory response and morphology were evaluated in vitro.
  
  
  
  Proliferation was insignificantly delayed by all the tested materials. Significant cytotoxicity was observed in presence of resin based composites (MTT assay), however no detectable apoptosis and some dead cells were detected like in PICN materials. Cell morphology, major cytoskeleton and extracellular matrix components were not altered. An intimate contact appeared between the materials and cells.
  
  
  
  The three new tested biomaterials did not exhibit adverse effects on oral stem cells in our experimental conditions and may be an interesting alternative to ceramics or composite based CAD-CAM blocks.
authors.affiliation: Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Cordeliers Research Center, 15 rue de l'école de médecine, Paris, F-75006, France; Paris-Descartes; Pierre and Marie Curie; Paris-Diderot Universities, Paris, F-75006, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Tassin
authors.affiliation: Faculty of Dentistry, Paris Descartes University, Sorbonne Paris Cité, France.|Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Cordeliers Research Center, 15 rue de l'école de médecine, Paris, F-75006, France; Paris-Descartes; Pierre and Marie Curie; Paris-Diderot Universities, Paris, F-75006, France.|Bretonneau Hospital, HUPNVS, AP-HP, Paris, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Bonte
authors.affiliation: Faculty of Dentistry, Paris Descartes University, Sorbonne Paris Cité, France.|Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Cordeliers Research Center, 15 rue de l'école de médecine, Paris, F-75006, France; Paris-Descartes; Pierre and Marie Curie; Paris-Diderot Universities, Paris, F-75006, France.
authors.forename: Ludwig S
authors.initials: LS
authors.lastname: Loison-Robert
authors.affiliation: Faculty of Dentistry, Paris Descartes University, Sorbonne Paris Cité, France.|Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Cordeliers Research Center, 15 rue de l'école de médecine, Paris, F-75006, France; Paris-Descartes; Pierre and Marie Curie; Paris-Diderot Universities, Paris, F-75006, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Nassif
authors.affiliation: Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Cordeliers Research Center, 15 rue de l'école de médecine, Paris, F-75006, France; Paris-Descartes; Pierre and Marie Curie; Paris-Diderot Universities, Paris, F-75006, France.
authors.forename: Tsouria
authors.initials: T
authors.lastname: Berbar
authors.affiliation: URB2i, Faculty of Dentistry, Paris Descartes University, Sorbonne Paris Cité, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Le Goff
authors.affiliation: Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Cordeliers Research Center, 15 rue de l'école de médecine, Paris, F-75006, France; Paris-Descartes; Pierre and Marie Curie; Paris-Diderot Universities, Paris, F-75006, France.|Faculty of Dentistry, Paris Diderot University, Sorbonne Paris Cité, France.
authors.forename: Ariane
authors.initials: A
authors.lastname: Berdal
authors.affiliation: Faculty of Dentistry, Paris Descartes University, Sorbonne Paris Cité, France.|URB2i, Faculty of Dentistry, Paris Descartes University, Sorbonne Paris Cité, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Sadoun
authors.affiliation: Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Cordeliers Research Center, 15 rue de l'école de médecine, Paris, F-75006, France; Paris-Descartes; Pierre and Marie Curie; Paris-Diderot Universities, Paris, F-75006, France.|Faculty of Dentistry, Paris Diderot University, Sorbonne Paris Cité, France.
authors.forename: Benjamin P J
authors.initials: BP
authors.lastname: Fournier
mesh_terms: D017667:Adipocytes / Q000166:cytology; D017209:Apoptosis; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D002516:Ceramics; D003470:Culture Media; D003782:Dental Pulp / Q000378:metabolism*; D005434:Flow Cytometry; D005881:Gingiva / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D007249:Inflammation; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D010637:Phenolsulfonphthalein / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011312:Pressure; D012117:Resins, Synthetic / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D011108:Polymers; D012117:Resins, Synthetic; D010637:Phenolsulfonphthalein