title: Aurora-A regulates MCRS1 function during mitosis.
abstract: The mitotic spindle is made of microtubules (MTs) nucleated through different pathways involving the centrosomes, the chromosomes or the walls of pre-existing MTs. MCRS1 is a RanGTP target that specifically associates with the chromosome-driven MTs protecting them from MT depolymerases. MCRS1 is also needed for the control of kinetochore fiber (K-fiber) MT minus-ends dynamics in metaphase. Here, we investigated the regulation of MCRS1 activity in M-phase. We show that MCRS1 is phosphorylated by the Aurora-A kinase in mitosis on Ser35/36. Although this phosphorylation has no role on MCRS1 localization to chromosomal MTs and K-fiber minus-ends, we show that it regulates MCRS1 activity in mitosis. We conclude that Aurora-A activity is particularly important in the tuning of K-fiber minus-ends dynamics in mitosis.
authors.affiliation: a Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Spain.|b Universitat Pompeu Fabra (UPF) , Barcelona , Spain.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Meunier
authors.affiliation: a Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Spain.|b Universitat Pompeu Fabra (UPF) , Barcelona , Spain.
authors.forename: Krystal
authors.initials: K
authors.lastname: Timón
authors.affiliation: a Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Spain.|b Universitat Pompeu Fabra (UPF) , Barcelona , Spain.|c Insitució Catalana de Recerca i Estudis Avançats (ICREA) , Barcelona , Spain.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vernos
mesh_terms: D064096:Aurora Kinase A / Q000378:metabolism*; D002877:Chromosomes, Human / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: C113292:MCRS1 protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D010768:Phosphoserine; D064096:Aurora Kinase A
keywords: Aurora-A kinase; chromosomal microtubules; K-fiber; MCRS1; microtubule minus-end; mitosis; phosphorylation