title: FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats.
abstract: Sphingosine-1-phosphate and its structural analog FTY720 (fingolimod) are important in the inhibition of osteoclast differentiation and bone resorption, however, it remains unknown whether they enhance osteogenic differentiation of the bone marrow mesenchymal stem cells (BM‑MSCs). The present study investigated the effect of FTY720 on the osteogenic differentiation of BM‑MSCs from the femurs of the ovariectomized (OVX) rats. Three different concentrations (1, 10 and 100 nM) of FTY720 were demonstrated to markedly upregulate mRNA expression levels of Runt‑related transcription factor 2 (Runx2) and Sp7 transcription factor (Sp7) at 2 weeks, and alkaline phosphatase (ALP) at 3 weeks. The osteocalcin (OCN) expression was similar at weeks 2 and 3. The protein expression levels of Runx2, Sp7, OCN and ALP induced by three different concentrations of FTY720 were higher than those in the control groups at 3 weeks in the OVX and sham groups. The findings of the current study suggested a beneficial effect of FTY720 on bone formation in OVX rats, and provided a potential therapeutic method of FTY720 to prevent alveolar bone resorption in patients with post‑menopausal osteoporosis.
authors.affiliation: Department of Orthodontics, State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ling
authors.affiliation: Department of Biomedical Engineering, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Fei-Jiang
authors.initials: FJ
authors.lastname: Li
authors.affiliation: Department of Gastroenterology and Endocrinology, Shaanxi Provincial Armed Police Corps Hospital, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Er-Cui
authors.initials: EC
authors.lastname: Li
authors.affiliation: Department of General Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Qi-Ke
authors.initials: QK
authors.lastname: Huang
authors.affiliation: Out‑Patient Department, General Hospital of The Second Artillery, Beijing 100820, P.R. China.
authors.forename: Bao-Gang
authors.initials: BG
authors.lastname: Liu
authors.affiliation: Department of Orthodontics, State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Ophthalmology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Si-Wei
authors.initials: SW
authors.lastname: You
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects*; D000066673:Cell Self Renewal / Q000187:drug effects / Q000235:genetics; D005260:Female; D000068876:Fingolimod Hydrochloride / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D010052:Ovariectomy; D051381:Rats
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D000068876:Fingolimod Hydrochloride