title: A DPA-based highly selective and sensitive fluorescent probe for mercuric ions and its imaging in living cells.
abstract: Based on the 2,2-dipicolylamine (DPA) receptor and naphthalimide fluorophore, three fluorescent probes, RDPA, MDPA and VDPA have been developed for the recognition of mercuric ions. Among them, VDPA, bearing a diglycol group in the imine chain, exhibits good water solubility, and high selectivity towards mercuric ions in PBS water solution. With DPA as the receptor, VDPA showed very high affinity and sensitivity towards Hg(2+), and the binding constant and detection limit were calculated to be 2.8 × 10(10) M(-1) and 5.49 nM, respectively. The MTT assay and living cell imaging experiments suggested that the probe VDPA has potential application for detecting Hg(2+) in living cells.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. wpzhu@ecust.edu.cn.
authors.forename: Junhua
authors.initials: J
authors.lastname: Jia
authors.forename: Luling
authors.initials: L
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Ding
authors.forename: Chusen
authors.initials: C
authors.lastname: Huang
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
mesh_terms: D000588:Amines / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004785:Environmental Pollutants / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000032:analysis; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D053644:Naphthalimides / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010848:Picolinic Acids / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: C501847:2,2'-dipicolylamine; D000588:Amines; D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D007477:Ions; D053644:Naphthalimides; D010848:Picolinic Acids; D014867:Water; D008628:Mercury