title: TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways.
abstract: The migration of circulating mesenchymal stem cells (MSCs) to injured tissue is an important step in tissue regeneration and requires adhesion to the microvascular endothelium. The current study investigated the underlying mechanism of MSC adhesion to endothelial cells during inflammation. In in vitro MSC culture, tumor necrosis factor‑α (TNF‑α) increased the level of vascular cell adhesion molecule‑1 (VCAM‑1) expression in a dose‑dependent manner. The nuclear factor-κB (NF-κB), extracellular signal‑regulated kinase (ERK) and c‑Jun N‑terminal kinase (JNK) signaling pathway inhibitors, pyrrolidine dithiocarbamate (PDTC), U0126 and SP600125, respectively, suppressed VCAM‑1 expression induced by TNF‑α at the mRNA and protein levels (P<0.05). TNF‑α augmented the activation of NF‑κB, ERK and JNK, and promoted MSC adhesion to human umbilical vein endothelial cells; however, the inhibitors of NF‑κB, ERK and JNK did not affect this process in these cells. The results of the current study indicate that adhesion of circulating MSCs to the endothelium is regulated by TNF-α-induced VCAM-1 expression, which is potentially mediated by the NF‑κB, ERK and JNK signaling pathways.
authors.affiliation: First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Zi-Yuan
authors.initials: ZY
authors.lastname: Lu
authors.affiliation: First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Wan-Cheng
authors.initials: WC
authors.lastname: Chen
authors.affiliation: Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Li
authors.affiliation: Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Pang
authors.affiliation: Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Xiao
authors.affiliation: Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Hao-Wen
authors.initials: HW
authors.lastname: Xiao
authors.affiliation: First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000873:Anthracenes / Q000494:pharmacology; D015415:Biomarkers; D002070:Butadienes / Q000494:pharmacology; D002448:Cell Adhesion; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D016130:Immunophenotyping; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D015415:Biomarkers; D002070:Butadienes; D016328:NF-kappa B; D009570:Nitriles; D014409:Tumor Necrosis Factor-alpha; C113580:U 0126; D019010:Vascular Cell Adhesion Molecule-1; C432165:pyrazolanthrone; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases