title: Paraburkholderia nodosa is the main N2-fixing species trapped by promiscuous common bean (Phaseolus vulgaris L.) in the Brazilian 'Cerradão'.
abstract: The bacterial genus Burkholderia comprises species occupying several habitats, including a group of symbionts of leguminous plants-also called beta-rhizobia-that has been recently ascribed to the new genus Paraburkholderia We used common bean (Phaseolus vulgaris L.) plants to trap rhizobia from an undisturbed soil of the Brazilian Cerrado under the vegetation type 'Cerradão'. Genetic characterization started with the analyses of 181 isolates by BOX-PCR, where the majority revealed unique profiles, indicating high inter- and intra-species diversity. Restriction fragment length polymorphism-PCR of the 16S rRNA of representative strains of the BOX-PCR groups indicated two main clusters, and gene-sequencing analysis identified the minority (27%) as Rhizobium and the majority (73%) as Paraburkholderia Phylogenetic analyses of the 16S rRNA and housekeeping (recA and gyrB) genes positioned all strains of the second cluster in the species P. nodosa, and the phylogeny of a symbiotic gene-nodC-was in agreement with the conserved genes. All isolates were stable vis-à-vis nodulating common bean, but, in general, with a low capacity for fixing N2, although some effective strains were identified. The predominance of P. nodosa might be associated with the edaphic properties of the Cerrado biome, and might represent an important role in terms of maintenance of the ecosystem, which is characterized by acid soils with high saturation of aluminum and low N2 content.
authors.affiliation: Embrapa Soja, Soil Biotechnology Laboratory, C.P. 231, 86001-970, Londrina, PR, Brazil Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, C.P. 10011, 86057-970, Londrina, PR, Brazil.
authors.forename: Rebeca F
authors.initials: RF
authors.lastname: Dall'Agnol
authors.affiliation: Embrapa Soja, Soil Biotechnology Laboratory, C.P. 231, 86001-970, Londrina, PR, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Plotegher
authors.affiliation: Embrapa Soja, Soil Biotechnology Laboratory, C.P. 231, 86001-970, Londrina, PR, Brazil Department of Microbiology, Universidade Federal do Paraná, C.P. 19031, 81531-990, Curitiba, PR, Brazil.
authors.forename: Renata C
authors.initials: RC
authors.lastname: Souza
authors.affiliation: Department of Soil Microbiology, Embrapa Cerrados, C.P. 08223, 73301-970, Planaltina, DF, Brazil.
authors.forename: Iêda C
authors.initials: IC
authors.lastname: Mendes
authors.affiliation: Department of Soil Microbiology, Embrapa Cerrados, C.P. 08223, 73301-970, Planaltina, DF, Brazil.
authors.forename: Fábio B
authors.initials: FB
authors.lastname: Dos Reis Junior
authors.affiliation: IRD, Cirad, University of Montpellier, Interactions Plantes Microorganismes Environnement (IPME), 34394 Montpellier, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Béna
authors.affiliation: IRD, Cirad, University of Montpellier, Interactions Plantes Microorganismes Environnement (IPME), 34394 Montpellier, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Moulin
authors.affiliation: Embrapa Soja, Soil Biotechnology Laboratory, C.P. 231, 86001-970, Londrina, PR, Brazil Department of Microbiology, Universidade Federal do Paraná, C.P. 19031, 81531-990, Curitiba, PR, Brazil mariangela.hungria@embrapa.br.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Hungria
mesh_terms: D020562:Betaproteobacteria / Q000502:physiology*; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D009586:Nitrogen Fixation / Q000502:physiology*; D027805:Phaseolus / Q000382:microbiology / Q000502:physiology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: Cerrado; beta-rhizobia; biological N2 fixation; phylogeny of prokaryotes