title: A novel systems pharmacology platform to dissect action mechanisms of traditional Chinese medicines for bovine viral diarrhea disease.
abstract: Due to the large direct and indirect productivity losses in the livestock industry caused by bovine viral diarrhea (BVD) and the lack of effective pharmacological therapies, developing an efficient treatment is extremely urgent. Traditional Chinese medicines (TCMs) that simultaneously address multiple targets have been proven to be effective therapies for BVD. However, the potential molecular action mechanisms of TCMs have not yet been systematically explored. In this work, take the example of a herbal remedy Huangqin Zhizi (HQZZ) for BVD treatment in China, a systems pharmacology approach combining with the pharmacokinetics and pharmacodynamics evaluation was developed to screen out the active ingredients, predict the targets and analyze the networks and pathways. Results show that 212 active compounds were identified. Utilizing these lead compounds as probes, we predicted 122 BVD related-targets. And in vitro experiments were conducted to evaluate the reliability of some vital active compounds and targets. Network and pathway analysis displayed that HQZZ was effective in the treatment of BVD by inhibiting inflammation, enhancing immune responses in hosts toward virus infection. In summary, the analysis of the complete profile of the pharmacological activities, as well as the elucidation of targets, networks and pathways can further elucidate the underlying anti-inflammatory, antiviral and immune regulation mechanisms of HQZZ against BVD.
authors.affiliation: Center of Bioinformatics, College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Center of Bioinformatics, College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China; College of Life Science, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Tianli
authors.initials: T
authors.lastname: Pei
authors.affiliation: Center of Bioinformatics, College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Center of Bioinformatics, College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Xuetong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Center of Bioinformatics, College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Yaofei
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Center of Bioinformatics, College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China; College of Life Science, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xue
authors.affiliation: Center of Bioinformatics, College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Ziyin
authors.initials: Z
authors.lastname: Wu
authors.affiliation: College of Life Science, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Jiexin
authors.initials: J
authors.lastname: Mu
authors.affiliation: School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Science, Northwest University, Xi'an, Shaanxi 710069, China. Electronic address: yh_wang@nwsuaf.edu.cn.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000930:Antidiarrheals / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000188:drug therapy / Q000378:metabolism*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008516:Medicine, Chinese Traditional / Q000379:methods*; D031364:Scutellaria baicalensis; D049490:Systems Biology / Q000379:methods*
chemical_list: D000930:Antidiarrheals; D004365:Drugs, Chinese Herbal
keywords: Baicalin (PubChem CID: 64982); Bovine viral diarrhea (BVD); Chrysin (PubChem CID: 5281607); Crocetin (PubChem CID: 5281232); Epicatechin (PubChem CID: 72276); Huangqin Zhizi formula; Isoimperatorin (PubChem CID: 68081); Magnoflorine (PubChem CID: 73337); Systems pharmacology; Therapeutic mechanisms; Traditional Chinese medicines