title: Anti-tumor activity of heat-killed Lactobacillus plantarum BF-LP284 on Meth-A tumor cells in BALB/c mice.
abstract: Probiotics exert numerous effects on human well-being. Here, heat-killed Lactobacillus plantarum BF-LP284 (H-Lp) was isolated as a potent immuno-modulator among 15 strains of lactobacilli in terms of TNF-α induction ability in peritoneal macrophages. In vitro TNF-α and IFN-γ induction in Peyer's patch (PP) cells was higher when incubated with H-Lp than with live L. plantarum BF-LP284 (L-Lp). Suppression of syngeneic Meth-A tumors in a murine model by oral administration of H-Lp was also greater than that of L-Lp and of controls. H-Lp stimulated IFN-γ production in spleen cells, which displayed inhibited tumor growth in Winn assays when treated with H-Lp. Moreover, H-Lp increased the ratio of CD3(+ )cells among peripheral blood mononuclear cells in Meth-A tumor-bearing mice, suggesting an H-Lp-mediated anti-tumor mechanism whereby immune cells that are activated by H-Lp in PP and acquire anti-tumor activity in the spleen migrate to tumor sites through lymphocyte homing to inhibit tumor growth.
authors.affiliation: a Central Institute for Health Science , A. L. A. Corporation , Tokyo , Japan ;|b Laboratory of Food Hygiene, School of Food Science and Technology , Nippon Veterinary and Life Science University , Tokyo , Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Shin
authors.affiliation: a Central Institute for Health Science , A. L. A. Corporation , Tokyo , Japan ;
authors.forename: Yukie
authors.initials: Y
authors.lastname: Itoh
authors.affiliation: a Central Institute for Health Science , A. L. A. Corporation , Tokyo , Japan ;
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Kataoka
authors.affiliation: a Central Institute for Health Science , A. L. A. Corporation , Tokyo , Japan ;
authors.forename: Shiori
authors.initials: S
authors.lastname: Iino-Miura
authors.affiliation: a Central Institute for Health Science , A. L. A. Corporation , Tokyo , Japan ;
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Miura
authors.affiliation: a Central Institute for Health Science , A. L. A. Corporation , Tokyo , Japan ;
authors.forename: Takeo
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: b Laboratory of Food Hygiene, School of Food Science and Technology , Nippon Veterinary and Life Science University , Tokyo , Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Fujisawa
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006358:Hot Temperature; D007371:Interferon-gamma / Q000378:metabolism; D048191:Lactiplantibacillus plantarum; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000382:microbiology; D017737:Macrophages, Peritoneal / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009374:Neoplasms, Experimental / Q000628:therapy*; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D019936:Probiotics; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
keywords: Heat-killed; IFN-γ; Lactobacillus plantarum; TNF-α; probiotics; tumor