title: Altered Cyclooxygenase-2 Expression in Pulmonary Sarcoidosis is not Related to Clinical Classifications.
abstract: Elevated COX-2 activity is associated with the development of chronic lung diseases leading to bronchial obstruction, including sarcoidosis. The aim of the study was to examine expression pattern of COX-2 messenger RNA (mRNA). Expression was performed by q-PCR method in bronchoalveolar lavage (BAL) cells and peripheral blood (PB) lymphocytes in sarcoidosis patients (n = 61) and control group (n = 30). Analysis of COX-2 mRNA expression level in BAL fluid and PB revealed downregulation in sarcoidosis and control groups. In PB lymphocytes, the statistically significant difference between patients and controls was observed (P = 0.003, Mann-Whitney U test), with higher expression in patients. There were no statistically significant differences between patients without and with parenchymal involvement (stages I vs. II-IV), between patients with acute vs. insidious onset of disease and between patients with abnormal vs. normal spirometry (P > 0.05, Mann-Whitney U test). Results suggest that expression of COX-2 mRNA in patients with pulmonary sarcoidosis is not related to clinical classifications.
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Kiszałkiewicz
authors.affiliation: Department of Pneumonology and Allergy, 1st Chair of Internal Diseases, Medical University of Lodz, St. Kopcińskiego 22, Lodz, 90-153, Poland.
authors.forename: Wojciech J
authors.initials: WJ
authors.lastname: Piotrowski
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Pastuszak-Lewandoska
authors.affiliation: Department of Pneumonology and Allergy, 1st Chair of Internal Diseases, Medical University of Lodz, St. Kopcińskiego 22, Lodz, 90-153, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Górski
authors.affiliation: Department of General and Oncological Pulmonology, 1st Chair of Internal Diseases, Medical University of Lodz, St. Kopcińskiego 22, Lodz, 90-153, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Antczak
authors.affiliation: Department of Pneumonology and Allergy, 1st Chair of Internal Diseases, Medical University of Lodz, St. Kopcińskiego 22, Lodz, 90-153, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Górski
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Daria
authors.initials: D
authors.lastname: Domańska-Senderowska
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Migdalska-Sęk
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Karolina H
authors.initials: KH
authors.lastname: Czarnecka
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Nawrot
authors.affiliation: Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland. ewa.brzezianska@umed.lodz.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzeziańska-Lasota
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016022:Case-Control Studies; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis*; D017565:Sarcoidosis, Pulmonary / Q000201:enzymology*
chemical_list: D012333:RNA, Messenger; D051546:Cyclooxygenase 2
keywords: COX-2; biomarkers; expression; pulmonary sarcoidosis