title: Comparing the energy landscapes for native folding and aggregation of PrP.
abstract: Protein sequences are evolved to encode generally one folded structure, out of a nearly infinite array of possible folds. Underlying this code is a funneled free energy landscape that guides folding to the native conformation. Protein misfolding and aggregation are also a manifestation of free-energy landscapes. The detailed mechanisms of these processes are poorly understood, but often involve rare, transient species and a variety of different pathways. The inherent complexity of misfolding has hampered efforts to measure aggregation pathways and the underlying energy landscape, especially using traditional methods where ensemble averaging obscures important rare and transient events. We recently studied the misfolding and aggregation of prion protein by examining 2 monomers tethered in close proximity as a dimer, showing how the steps leading to the formation of a stable aggregated state can be resolved in the single-molecule limit and the underlying energy landscape thereby reconstructed. This approach allows a more quantitative comparison of native folding versus misfolding, including fundamental differences in the dynamics for misfolding. By identifying key steps and interactions leading to misfolding, it should help to identify potential drug targets. Here we describe the importance of characterizing free-energy landscapes for aggregation and the challenges involved in doing so, and we discuss how single-molecule studies can help test proposed structural models for PrP aggregates.
authors.affiliation: a Department of Physics , University of Alberta , Edmonton , AB , Canada;
authors.forename: Derek R
authors.initials: DR
authors.lastname: Dee
authors.affiliation: a Department of Physics , University of Alberta , Edmonton , AB , Canada;|b National Institute for Nanotechnology, National Research Council , Edmonton , AB , Canada.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Woodside
mesh_terms: D000818:Animals; D006224:Cricetinae; D006801:Humans; D017096:Prion Diseases / Q000378:metabolism; D011328:Prions / Q000737:chemistry* / Q000378:metabolism; D066329:Protein Aggregates; D066263:Protein Aggregation, Pathological / Q000378:metabolism; D017510:Protein Folding; D055503:Protein Multimerization; D013816:Thermodynamics
chemical_list: D011328:Prions; D066329:Protein Aggregates
keywords: energy landscape; optical tweezers; prion protein; protein aggregation; single molecule