title: Diagnostic performances of antigen detection compared to conventional and nucleic acid detection of Entamoeba histolytica in a non-endemic setting.
abstract: This study evaluated the immunochromatographic (IC) assay "TECHLAB(®) E. HISTOLYTICA QUIK CHEK™" analysing 36 faecal samples and 7 cultured strains. This assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. The IC assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity with other parasites and failed to detect E. histolytica antigen in frozen faeces. In our laboratory located in a non-endemic setting this assay could not replace the methods currently used for the diagnosis of amoebiasis.
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Calderaro
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Piergianni
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Piccolo
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Sabina
authors.initials: S
authors.lastname: Rossi
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Montecchini
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Mirko
authors.initials: M
authors.lastname: Buttrini
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Arcangeletti
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Medici
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Chezzi
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Flora
authors.initials: F
authors.lastname: De Conto
mesh_terms: D000953:Antigens, Protozoan / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis* / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan
keywords: Antigen detection; Diagnosis; Entamoeba histolytica; Real-time PCR