title: Enhancing the Activity of Peptide-Based Artificial Hydrolase with Catalytic Ser/His/Asp Triad and Molecular Imprinting.
abstract: In this study, an artificial hydrolase was developed by combining the catalytic Ser/His/Asp triad with N-fluorenylmethoxycarbonyl diphenylalanine (Fmoc-FF), followed by coassembly of the peptides into nanofibers (CoA-HSD). The peptide-based nanofibers provide an ideal supramolecular framework to support the functional groups. Compared with the self-assembled catalytic nanofibers (SA-H), which contain only the catalytic histidine residue, the highest activity of CoA-HSD occurs when histidine, serine, and aspartate residues are at a ratio of 40:1:1. This indicates that the well-ordered nanofiber structure and the synergistic effects of serine and aspartate residues contribute to the enhancement in activity. Additionally, for the first time, molecular imprinting was applied to further enhance the activity of the peptide-based artificial enzyme (CoA-HSD). p-NPA was used as the molecular template to arrange the catalytic Ser/His/Asp triad residues in the proper orientation. As a result, the activity of imprinted coassembled CoA-HSD nanofibers is 7.86 times greater than that of nonimprinted CoA-HSD and 13.48 times that of SA-H.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, P. R. China.|Tianjin Key Laboratory of Membrane Science and Desalination Technology , Tianjin 300072, P. R. China.
authors.forename: Mengfan
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, P. R. China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, P. R. China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, P. R. China.|Tianjin Key Laboratory of Membrane Science and Desalination Technology , Tianjin 300072, P. R. China.|The Co-Innovation Centre of Chemistry and Chemical Engineering of Tianjin, Tianjin 300072, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, P. R. China.|Tianjin Key Laboratory of Membrane Science and Desalination Technology , Tianjin 300072, P. R. China.|The Co-Innovation Centre of Chemistry and Chemical Engineering of Tianjin, Tianjin 300072, P. R. China.
authors.forename: Rongxin
authors.initials: R
authors.lastname: Su
authors.affiliation: State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, P. R. China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000737:chemistry; D002384:Catalysis; D006639:Histidine / Q000737:chemistry; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D054802:Molecular Imprinting; D010455:Peptides / Q000737:chemistry*; D012694:Serine / Q000737:chemistry
chemical_list: D010455:Peptides; D001224:Aspartic Acid; D012694:Serine; D006639:Histidine; D006867:Hydrolases
keywords: artificial enzyme; catalytic triad; molecular imprinting; nanofiber; peptide assembly