title: miR-410-3p suppresses breast cancer progression by targeting Snail.
abstract: miR-410-3p acts as an oncogene or tumor-suppressor gene in various types of cancer. However, its role in breast cancer remains unknown. In the present study, expression of miR-410-3p in 30 breast cancer and paired adjacent normal tissues was detected by RT-qPCR. The expression of miR-410-3p was downregulated in 76.7% of the breast cancer samples. To further validate the expression of miR-410-3p in breast cancer, we analyzed miR-410-3p expression profiling data set from The Cancer Genome Atlas (TCGA) including 683 breast cancer and 87 normal breast tissues. We observed that the expression of miR-410-3p was downregulated in breast cancer tissues. Next, we investigated the influence of miR-410-3p on cell proliferation by transiently transfecting the miR-410-3p mimic or inhibitor, as well as their corresponding controls in the MDA-MB-231 and MCF7 cell lines. miR-410-3p overexpression reduced cell growth, colony formation and the number of EdU-positive cells in the MDA-MB-231 cells. In contrast, inhibition of miR-410-3p in the MCF7 cells resulted in a higher proliferation rate as assessed by MTT assay, plate colony formation and EdU assays. Furthermore, miR-410-3p inhibited epithelial-mesenchymal transition. In addition, Snail was found to be a direct target of miR-410-3p based on a luciferase assay. Overexpression of Snail was able to rescue the effect of miR-410-3p in breast cancer cells. Moreover, miR‑410-3p was inversely expressed with Snail in breast cancer samples. Our data provide new knowledge regarding the role of miR-410-3p in breast cancer progression.
authors.affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, P.R. China.
authors.forename: Wang-Zhao
authors.initials: WZ
authors.lastname: Song
authors.affiliation: Department of Surgery, Affiliated Hospital, Inner Mongolia Medical University, Inner Mongolia 010050, P.R. China.
authors.forename: Rui-Ming
authors.initials: RM
authors.lastname: Zhang
authors.affiliation: Department of Surgery, Affiliated Hospital, Inner Mongolia Medical University, Inner Mongolia 010050, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Surgery, Affiliated Hospital, Inner Mongolia Medical University, Inner Mongolia 010050, P.R. China.
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Bai
authors.affiliation: Department of Surgery, Affiliated Hospital, Inner Mongolia Medical University, Inner Mongolia 010050, P.R. China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Kang
authors.affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: The First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.
authors.forename: Xu-Chen
authors.initials: XC
authors.lastname: Cao
mesh_terms: D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D000071250:Snail Family Transcription Factors / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics
chemical_list: C568527:MIRN410 microRNA, human; D035683:MicroRNAs; D000071250:Snail Family Transcription Factors