title: Brief Communication: SIR-2.1-dependent lifespan extension of Caenorhabditis elegans by oxyresveratrol and resveratrol.
abstract: Resveratrol (RES) has been studied for its effects on the lifespan extension of Caenorhabditis elegans, but controversy still remains on its mechanism related with SIR-2. In this study, longevity assay was performed to confirm SIR-2-dependent lifespan extension of C. elgeans with RES and oxyresveratrol (OXY), an isomer of hydroxylated RES using loss-of-function mutants of C. elegans including sir-2.1 mutant. The results showed that OXY and RES significantly (P < 0.05) extended the lifespan of C. elegans compared with the control. OXY and RES also significantly (P < 0.05) increased the mRNA expression levels of sir-2.1 and aak-2 in a dose-dependent manner and increased the protein expression levels of SIR-2.1. OXY and RES treatment extended the lifespan in daf-16 loss-of-function mutants, which suggested that lifespan extension was not occurring via the activation of DAF-16. However, OXY and RES failed to extend the lifespan in loss-of-function mutants of sir-2.1 and aak-2 Therefore, OXY and RES extend the lifespan of C. elegans by overexpression of SIR-2.1, which is related to lifespan extension through calorie restriction and the AMP-activated protein kinase (AMPK) pathway, although this process is independent of the FOXO/DAF-16 pathway.
authors.affiliation: Department of Public Health Science (Brain Korea 21 PLUS program), Graduate School, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Jiyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Public Health Science (Brain Korea 21 PLUS program), Graduate School, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Gayeung
authors.initials: G
authors.lastname: Kwon
authors.affiliation: Department of Public Health Science (Brain Korea 21 PLUS program), Graduate School, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, Shihung-si, Gyeonggi-do 429-793, Republic of Korea.
authors.forename: Jeong-Keun
authors.initials: JK
authors.lastname: Kim
authors.affiliation: Department of Public Health Science (Brain Korea 21 PLUS program), Graduate School, Korea University, Seoul 136-701, Republic of Korea School of Biosystem and Biomedical Science, College of Health Science, Korea University, Seoul 136-701, Republic of Korea Department of Laboratory Medicine, Korea University Guro Hospital, Seoul 152-703, Republic of Korea yhlim@korea.ac.kr.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lim
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D008136:Longevity / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D000077185:Resveratrol; D037761:Sirtuins / Q000187:drug effects / Q000502:physiology*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; C507014:SIR-2.1 protein, C elegans; D037761:Sirtuins; D000077185:Resveratrol
keywords: Caenorhabditis elegans; Oxyresveratrol; SIR-2.1; lifespan; resveratrol