title: Matrix-assisted laser desorption ionization-time of flight Mass spectrometry can detect Staphylococcus aureus clonal complex 398.
abstract: Within the last decade methicillin-resistant Staphylococcus aureus belonging to CC398 has become a worldwide threat associated with livestock. More recently, methicillin-susceptible S. aureus (MSSA) belonging to CC398 have been increasingly reported as a cause of invasive infections in patients without livestock contact. It appears therefore necessary to implement a convenient tool for the surveillance this emerging pathogen. We evaluated the MALDI-TOF MS as a tool for rapid detection of S. aureus CC398. We used 626 S. aureus isolates characterized by a CC398-specific PCR, to constitute independent training (300 isolates including 60 isolates CC398) and validation sets (326 isolates including 82 isolates CC398). Fifteen peak biomarkers of CC398 were identified from the mass spectra of the training set. Ninety four % (307 of 326) of strains of the validation set were well assigned with an overall sensitivity of 93% and a specificity of 95%. Six CC398 and 13 non-CC398 isolates were misclassified. With MALDI-TOF MS, clinical laboratories could rapidly detect S. aureus CC398 associated with a higher mortality in hospitalized patients.
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-Environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Filière microbiologie, Centre Hospitalier Régional Universitaire, Besançon, France. Electronic address: msauget@chu-besancon.fr.
authors.forename: Marlène
authors.initials: M
authors.lastname: Sauget
authors.affiliation: Service de Bactériologie et Hygiène, UMR1282 ISP, Centre Hospitalier Régional Universitaire, Tours, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: van der Mee-Marquet
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-Environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Bertrand
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-Environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Filière microbiologie, Centre Hospitalier Régional Universitaire, Besançon, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Hocquet
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D015415:Biomarkers / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000302:isolation & purification*
chemical_list: D015415:Biomarkers
keywords: Bruker; MALDI-TOF MS; Mass spectrometry; S. aureus; Typing