title: Wenyingzhuangia fucanilytica sp. nov., a sulfated fucan utilizing bacterium isolated from shallow coastal seawater.
abstract: A Gram-staining-negative, rod-shaped, strictly aerobic, non-flagellated, non-gliding and yellow-pigmented bacterial strain, designated as strain CZ1127T, capable of utilizing sulfated fucan, was isolated from shallow coastal seawater of Jiaozhou Bay, Qingdao, PR China. Its taxonomic position was investigated by a polyphasic approach. CZ1127T grew at 15-37 °C (optimum, 25 °C), pH 5.5-9.0 (optimum, pH 6.5-7.5) and in the presence of 0-4 % (w/v) NaCl [optimum, 0-3 % (w/v)]. CZ1127T contained MK-6 as the sole menaquinone, and iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 1ω7c/C16 : 1ω6c and iso-C15 : 0 3-OH as its major fatty acids. The DNA G+C content of strain CZ1127T was 32.2 mol%. Phylogenetic analysis based on the 16S rRNA gene indicated that strain CZ1127T clustered with members of the genus Wenyingzhuangia and was closely related to Wenyingzhuangia marina CGMCC 1.12162T (97.4 % 16S rRNA gene sequence similarity) and Wenyingzhuangia gracilariae KCTC 42246T (96.1 %). The DNA-DNA relatedness between strain CZ1127T and W. marina CGMCC 1.12162T was 41.9±1.5 %. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, CZ1127T represents a novel species of the genus Wenyingzhuangia, for which the name Wenyingzhuangia fucanilytica sp. nov. is proposed. The type strain is CZ1127T (=CCTCC AB 2015089T=KCTC 42864T).
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Food Science, University of Massachusetts, 100 Holdsworth Way, Amherst, MA, 01003, USA.|College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, PR China.
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: Chang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, PR China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Dong
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, PR China.
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6; C007789:fucoidan