title: Long non-coding RNA metastasis associated in lung adenocarcinoma transcript 1 (MALAT1) interacts with estrogen receptor and predicted poor survival in breast cancer.
abstract: Metastasis associated in lung adenocarcinoma transcript 1 (MALAT1), a lncRNA that was first recognized as a prognostic parameter for patient survival of stage I lung cancer, is up-regulated in multiple human malignancies, including breast cancer. However, the mechanism of its function remained elusive. In the current study, by examining MALAT1 expression on mRNA level, we demonstrated that compared with MCF10A, MALAT1 expression was up-regulated in the majority of breast cancer cell lines (9/12). In 26 pairs of estrogen receptor (ER)-positive breast cancer samples, MALAT1 expression was significantly up-regulated compared with adjacent normal tissues (P = 0.012). Furthermore, of 204 breast cancer patients, high MALAT1 expression was associated with positive ER (P = 0.023) and progesterone receptor (PR) (P = 0.024) status. Further analysis using TCGA database revealed that ER and its target genes PGR and CCND1, were overexpressed in MALAT1 altered group compared with unaltered group, both on the mRNA and protein level. Lastly, we verified MALAT1's prognostic value in breast cancer. At the cut-off value of 75%, MALAT1 was the only independent prognostic factor of recurrence-free survival (RFS) in ER-negative patients in a multivariate Cox regression model (hazard ratio [HR] = 2.83, 95% confidence interval [CI] 1.02-7.83). MALAT1 overexpression was also associated with poor RFS in tamoxifen treated ER-positive breast cancer patients, which might serve as a potential biomarker to predict endocrine treatment sensitivity.
authors.affiliation: Department of Breast Surgery, Fudan University Shanghai Cancer Center, China.|Department of Oncology, Shanghai Medical College, Fudan University, China.
authors.forename: Nai-Si
authors.initials: NS
authors.lastname: Huang
authors.affiliation: Department of Breast Surgery, Fudan University Shanghai Cancer Center, China.|Department of Oncology, Shanghai Medical College, Fudan University, China.
authors.forename: Ya-Yun
authors.initials: YY
authors.lastname: Chi
authors.affiliation: Department of Breast Surgery, Fudan University Shanghai Cancer Center, China.|Department of Oncology, Shanghai Medical College, Fudan University, China.
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Xue
authors.affiliation: Department of Breast Surgery, Fudan University Shanghai Cancer Center, China.|Department of Oncology, Shanghai Medical College, Fudan University, China.
authors.forename: Meng-Ying
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Department of Breast Surgery, Fudan University Shanghai Cancer Center, China.|Department of Oncology, Shanghai Medical College, Fudan University, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University, China.|Department of Clinical Statistics, Fudan University Shanghai Cancer Center, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Mo
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University, China.|Department of Pathology, Fudan University Shanghai Cancer Center, China.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Zhou
authors.affiliation: Department of Breast Surgery, Fudan University Shanghai Cancer Center, China.|Department of Oncology, Shanghai Medical College, Fudan University, China.|Collaborative Innovation Center of Cancer Medicine, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Wu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D011960:Receptors, Estrogen
keywords: MALAT1; breast cancer; estrogen receptor; long non-coding RNA