title: Biosensor based on electrospun blended chitosan-poly (vinyl alcohol) nanofibrous enzymatically sensitized membranes for pirimiphos-methyl detection in olive oil.
abstract: An ultra-sensitive electrochemical biosensor was successfully developed for rapid detection of pirimiphos-methyl in olive oil, based of genetically-engineered acetylcholinesterase (AChE) immobilization into electrospun chitosan/poly (vinyl alcohol) blend nanofibers. Due to their unique properties such as spatial structure, high porosity, and large surface area, the use of nanofibers allowed improving the biosensor response by two folds. The developed biosensor showed a good performance for detecting pirimiphos-methyl, with a limit of detection of 0.2nM, a concentration much lower than the maximum residue limit allowed set by international regulations (164nM). The biosensor was used for the detection of pirimiphos-methyl in olive oil samples after a simple liquid-liquid extraction, and the recovery rates were close to 100%.
authors.affiliation: Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt.
authors.forename: A Y
authors.initials: AY
authors.lastname: El-Moghazy
authors.affiliation: Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt.
authors.forename: E A
authors.initials: EA
authors.lastname: Soliman
authors.affiliation: Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria 832, Egypt.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Ibrahim
authors.affiliation: BAE: Biocapteurs-Analyses-Environnment, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors.forename: J-L
authors.initials: JL
authors.lastname: Marty
authors.affiliation: BAE: Biocapteurs-Analyses-Environnment, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors.forename: G
authors.initials: G
authors.lastname: Istamboulie
authors.affiliation: BAE: Biocapteurs-Analyses-Environnment, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors.forename: T
authors.initials: T
authors.lastname: Noguer
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D000122:Acetylthiocholine / Q000737:chemistry; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D004560:Electricity; D004593:Electrophorus; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry; D000069463:Olive Oil / Q000737:chemistry*; D009946:Organothiophosphorus Compounds / Q000032:analysis*; D011142:Polyvinyl Alcohol / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D000069463:Olive Oil; D009946:Organothiophosphorus Compounds; C014153:pirimiphos methyl; D000122:Acetylthiocholine; D011142:Polyvinyl Alcohol; D048271:Chitosan; D000110:Acetylcholinesterase
keywords: Biosensor; Electrospinning; Genetically-Modified Acetylcholinesterase; Nanofibers; Olive Oil; Organophosphorus Pesticides