title: Binding activities of non-β-glucan glycoclusters to dectin-1 and exploration of their binding site.
abstract: Dectin-1, which specifically recognizes β-(1,3)-glucans, plays an important role in innate immune responses. For the first time, in this study we found that a series of non-β-glucan glycoclusters can bind to dectin-1 by means of surface plasmon resonance (SPR) assay. Hexavalent lactoside Ju-6 showed the strongest affinity property (KD=1.6 µM). Interestingly, a continuous binding-dissociation experiment on SPR showed that Ju-6 and Laminarin binding to dectin-1 are independent of each other. Moreover, RT-PCR assay showed that Ju-6 cannot up-regulate cytokine gene expression or inhibit the promoting effect caused by Zymosan (a long-chain β-glucan). These results indicated that there might be a possible new carbohydrate binding site on dectin-1.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road, Haidian District, Beijing 100191, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road, Haidian District, Beijing 100191, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road, Haidian District, Beijing 100191, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road, Haidian District, Beijing 100191, China. Electronic address: zjli@bjmu.edu.cn.
authors.forename: Zhong-Jun
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road, Haidian District, Beijing 100191, China. Electronic address: qli@bjmu.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D001665:Binding Sites; D002240:Carbohydrate Sequence; D005786:Gene Expression Regulation; D005936:Glucans / Q000737:chemistry* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D037181:Lectins, C-Type / Q000737:chemistry* / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D061251:Primary Cell Culture; D011485:Protein Binding; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015054:Zymosan / Q000737:chemistry / Q000494:pharmacology; D000099181:Dectin-1; D000093242:Interleukin-6 Inhibitors
chemical_list: D005936:Glucans; D006027:Glycosides; D018664:Interleukin-12; D015850:Interleukin-6; D037181:Lectins, C-Type; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D015054:Zymosan; D000099181:Dectin-1; D000093242:Interleukin-6 Inhibitors; C508600:IL6 protein, human; C008245:lactosides; C008247:laminaran
keywords: Binding affinity; Binding site; Dectin-1; Non-β-glucan glycocluster