title: Regulation of fibrillins and modulators of TGFβ in fetal bovine and human ovaries.
abstract: Fibrillins 1-3 are stromal extracellular matrix proteins that play important roles in regulating TGFβ activity, which stimulates fibroblasts to proliferate and synthesize collagen. In the developing ovary, the action of stroma is initially necessary for the formation of ovigerous cords and subsequently for the formation of follicles and the surface epithelium of the ovary. FBN3 is highly expressed only in early ovarian development and then it declines. In contrast, FBN1 and 2 are upregulated in later ovarian development. We examined the expression of FBN1-3 in bovine and human fetal ovaries. We used cell dispersion and monolayer culture, cell passaging and tissue culture. Cells were treated with growth factors, hormones or inhibitors to assess the regulation of expression of FBN1-3 When bovine fetal ovarian tissue was cultured, FBN3 expression declined significantly. Treatment with TGFβ-1 increased FBN1 and FBN2 expression in bovine fibroblasts, but did not affect FBN3 expression. Additionally, in cultures of human fetal ovarian fibroblasts (9-17weeks gestational age), the expression of FBN1 and FBN2 increased with passage, whereas FBN3 dramatically decreased. Treatment with activin A and a TGFβ family signaling inhibitor, SB431542, differentially regulated the expression of a range of modulators of TGFβ signaling and of other growth factors in cultured human fetal ovarian fibroblasts suggesting that TGFβ signaling is differentially involved in the regulation of ovarian fibroblasts. Additionally, since the changes in FBN1-3 expression that occur in vitro are those that occur with increasing gestational age in vivo, we suggest that the fetal ovarian fibroblasts mature in vitro.
authors.affiliation: Discipline of Obstetrics and GynaecologySchool of Medicine, Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Bastian
authors.affiliation: Medical Research Council Centre for Reproductive HealthUniversity of Edinburgh, Queen's Medical Research Institute, Edinburgh, UK.
authors.forename: Rosemary A
authors.initials: RA
authors.lastname: Bayne
authors.affiliation: Discipline of Obstetrics and GynaecologySchool of Medicine, Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Katja
authors.initials: K
authors.lastname: Hummitzsch
authors.affiliation: Discipline of Obstetrics and GynaecologySchool of Medicine, Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Hatzirodos
authors.affiliation: Discipline of Obstetrics and GynaecologySchool of Medicine, Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Wendy M
authors.initials: WM
authors.lastname: Bonner
authors.affiliation: Discipline of Obstetrics and GynaecologySchool of Medicine, Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Monica D
authors.initials: MD
authors.lastname: Hartanti
authors.affiliation: Discipline of Obstetrics and GynaecologySchool of Medicine, Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia School of Medical ScienceGriffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Helen F
authors.initials: HF
authors.lastname: Irving-Rodgers
authors.affiliation: Medical Research Council Centre for Reproductive HealthUniversity of Edinburgh, Queen's Medical Research Institute, Edinburgh, UK.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Anderson
authors.affiliation: Discipline of Obstetrics and GynaecologySchool of Medicine, Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia ray.rodgers@adelaide.edu.au.
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Rodgers
mesh_terms: D028341:Activins / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D000071838:Fibrillin-1 / Q000378:metabolism; D000071840:Fibrillin-2 / Q000378:metabolism; D000071837:Fibrillins / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D054392:Adipokines
chemical_list: D028341:Activins; D000071838:Fibrillin-1; D000071840:Fibrillin-2; D000071837:Fibrillins; D016212:Transforming Growth Factor beta; C000605722:FBN1 protein, human; D054392:Adipokines; C000605723:FBN2 protein, human; C493782:FBN3 protein, human; C422405:activin A