title: 4-Vinylphenol production from glucose using recombinant Streptomyces mobaraense expressing a tyrosine ammonia lyase from Rhodobacter sphaeroides.
abstract: OBJECTIVE
  To find a novel host for the production of 4-vinylphenol (4VPh) by screening Streptomyces species.
  
  
  RESULTS
  The conversion of p-coumaric acid (pHCA) to 4VPh in Streptomyces mobaraense was evaluated using a medium containing pHCA. S. mobaraense readily assimilated pHCA after 24 h of cultivation to produce 4VPh. A phenolic acid decarboxylase, derived from S. mobaraense (SmPAD), was purified following heterologous expression in Escherichia coli. SmPAD was evaluated under various conditions, and the enzyme's kcat/Km value was 0.54 mM (-1) s(-1). Using intergenetic conjugation, a gene from Rhodobacter sphaeroides encoding a tyrosine ammonia lyase, which catalyzes the conversion of L-tyrosine to p-coumaric acid, was introduced into S. mobaraense. The resulting S. mobaraense transformant produced 273 mg 4VPh l(-1) from 10 g glucose l(-1).
  
  
  CONCLUSIONS
  A novel strain suitable for the production of 4VPh and potentially other aromatic compounds was isolated.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fujiwara
authors.affiliation: Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Noda
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kawai
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan. akondo@kobe-u.ac.jp.|Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000642:Ammonia-Lyases / Q000235:genetics / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011422:Propionates; D012242:Rhodobacter sphaeroides / Q000201:enzymology*; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D003373:Coumaric Acids; D010636:Phenols; D011422:Propionates; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; C495469:p-coumaric acid; D005947:Glucose; C030626:4-vinylphenol
keywords: 4-Vinylphenol; Intergenetic conjugation; Phenolic acids; Streptomyces mobaraense; p-Coumaric acid