title: Simvastatin downregulated C35 expression and inhibited the proliferation of colon cancer cells Lovo and HT29 in vitro.
abstract: The aim of this study was to investigate the antitumor effect of simvastatin in human colon cancer and the possible underlying mechanism. We found that simvastatin dose-dependently inhibited the proliferation of human colon cancer cells Lovo and HT29 using a MTT assay. Real-time PCR and Western blotting assays showed that simvastatin significantly suppressed C35 expression at both mRNA and protein levels. Since C35 is known to have a significant oncogenic role in cancer development via promoting cell proliferation and migration, results obtained in the current study imply that downregulation of C35 expression might be involved in the antitumor effect of simvastatin on colon cancer.
authors.affiliation: Shandong Academy of Medicine Sciences, Shandong Provincial Institute of Parasitic Diseases.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Xuan
authors.initials: X
authors.lastname: Dong
authors.forename: Qingkuan
authors.initials: Q
authors.lastname: Wei
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Chenchen
authors.initials: C
authors.lastname: Li
authors.forename: Conghu
authors.initials: C
authors.lastname: Qi
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D019821:Simvastatin / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; C517054:MIEN1 protein, human; D009363:Neoplasm Proteins; D019821:Simvastatin