title: Reductions in tonic GABAergic current in substantia gelatinosa neurons and GABAAreceptor δ subunit expression after chronic constriction injury of the sciatic nerve in mice.
abstract: Decreased Gamma-aminobutyric acid (GABA)-ergic phasic inhibitory transmission in the spinal cord is thought to be responsible for the development of neuropathic pain. However, the role of GABAergic tonic current in substantia gelatinosa (SG) neurons in neuropathic pain remains to be fully elucidated. In this study, we assessed GABAergic tonic currents of SG neurons in a sciatic nerve chronic constriction injury (CCI) mouse.
  
  
  
  Whole-cell patch clamp recordings form lumbar spinal cord slices was performed to evaluate GABAergic currents. We also investigated the expression changes of GABAAreceptor subunits which are considered to mediate tonic currents.
  
  
  
  The percentage of SG neurons receiving GABAergic tonic currents decreased in CCI mice compared with Naïve mice. No significant change was observed in the mean amplitude of GABAergic tonic currents. RT-PCR and Western blot revealed that the expression of GABAAreceptor δ subunits decreased following CCI.
  
  
  
  A reduction in the expression the δ subunit of the GABAAreceptor and diminished GABAergic tonic current in SG neurons were observed after CCI in mice. GABAergic tonic current plays a key role in neuropathic pain. The GABAAreceptor δ subunit may be a therapeutic target in neuropathic pain. WHAT DOES THIS STUDY ADD?: In spinal SG neurons, GABAergic inhibitory transmission operates through both phasic and tonic currents, but physiological role is largely unknown. In this study, we report dysregulation of GABAAreceptor δ subunit-mediated tonic current in SG neurons may result in spinal disinhibition resulting in neuropathic pain in CCI mice.
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Graduate School of Medicine, Osaka University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Iura
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Graduate School of Medicine, Osaka University, Japan. atakahashi@anes.med.osaka-u.ac.jp.
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Graduate School of Medicine, Osaka University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hakata
authors.affiliation: Toyonaka Municipal Hospital, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mashimo
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Graduate School of Medicine, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujino
mesh_terms: D000818:Animals; D003250:Constriction; D004195:Disease Models, Animal; D008159:Lumbar Vertebrae; D051379:Mice; D009437:Neuralgia / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control; D009474:Neurons / Q000378:metabolism; D018408:Patch-Clamp Techniques; D059348:Peripheral Nerve Injuries / Q000209:etiology / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000502:physiology*; D012584:Sciatic Nerve / Q000293:injuries*; D013376:Substantia Gelatinosa / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid