title: Truncations of xyloglucan xylosyltransferase 2 provide insights into the roles of the N- and C-terminus.
abstract: Xyloglucan is the most abundant hemicellulose in the primary cell wall of dicotyledonous plants. In Arabidopsis, three xyloglucan xylosyltransferases, XXT1, XXT2, and XXT5, participate in xylosylation of the xyloglucan backbone. Despite the importance of these enzymes, there is a lack of information on their structure and the critical residues required for substrate binding and transferase activity. In this study, the roles of different domains of XX2 in protein expression and catalytic activity were investigated by constructing a series of N- and C-terminal truncations. XXT2 with an N-terminal truncation of 31 amino acids after the predicted transmembrane domain showed the highest protein expression, but truncations of more than 31 residues decreased protein expression and catalytic activity. XXT2 constructs with C-terminal truncations showed increased protein expression but decreased activity, particularly for truncations of 44 or more amino acids. Site-directed mutagenesis was also used to investigate six positively charged residues near the C-terminus and found that four of the mutants showed decreased enzymatic activity. We conclude that the N- and C-termini of XXT2 have important roles in protein folding and enzymatic activity: the stem region (particularly the N-terminus of the catalytic domain) is critical for protein folding and the C-terminus is essential for enzymatic activity but not for protein folding.
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Alan T
authors.initials: AT
authors.lastname: Culbertson
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Adrienne L
authors.initials: AL
authors.lastname: Smith
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Cook
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA. Electronic address: zabotina@iastate.edu.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Zabotina
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D005936:Glucans / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D010430:Pentosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D014990:Xylans / Q000737:chemistry*
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D010430:Pentosyltransferases; C029368:xyloglucan xylosyltransferase
keywords: Arabidopsis thalania; Truncated proteins; Xyloglucan; Xyloglucan xylosyltransferase activity