title: Protective Effect of Cod (Gadus macrocephalus) Skin Collagen Peptides on Acetic Acid-Induced Gastric Ulcer in Rats.
abstract: This research was performed to explore the protective effect of cod skin collagen peptides (CCP) on gastric ulcer induced by acetic acid. The CCP were fractionated into low molecular CCP (LMCCP, Mw < 3 kDa) and high molecular CCP (HMCCP, Mw > 3 kDa). In HMCCP and LMCCP, glycine of accounted for about one-third of the total amino acids without cysteine and tryptophan, and hydrophobic amino acids accounted for about 50%. After 21 d CCP treatment (60 or 300 mg/kg, p.o./daily), the healing effects on acetic acid-induced gastric ulcers were evaluated by macroscopic measure, microscopic measure, and immune histochemistry. Moreover, the expression levels of the growth factors, such as vascular endothelial growth factor, epidermal growth factor, transforming growth factor β1 (TGFβ1), and the heat shock protein 70 (HSP70) was detected. The results showed that both LMCCP and HMCCP could significantly decrease the ulcer areas and promote the healing of the lesions. They also could improve the levels of hexosamine, glutathione, superoxide dismutase, and glutathione peroxidase, and reduce the content of malondialdehyde and inducible nitric oxide synthase. In addition, the expression level of TGFβ1 gene and HSP70 mRNA was significantly improved by the treatment. It suggested that CCP could be able to improve symptoms of gastric ulcer and probably be used in the treatment of gastric ulcer.
authors.affiliation: College of Food Science and Engineering, Ocean Univ. of China, No. 5, Yu Shan Road, Qingdao, Shandong Province, 266003, P.R. China.
authors.forename: Huina
authors.initials: H
authors.lastname: Niu
authors.affiliation: College of Food Science and Engineering, Ocean Univ. of China, No. 5, Yu Shan Road, Qingdao, Shandong Province, 266003, P.R. China.
authors.forename: Zhicong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean Univ. of China, No. 5, Yu Shan Road, Qingdao, Shandong Province, 266003, P.R. China.
authors.forename: Hu
authors.initials: H
authors.lastname: Hou
authors.affiliation: College of Food Science and Engineering, Ocean Univ. of China, No. 5, Yu Shan Road, Qingdao, Shandong Province, 266003, P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Ocean Univ. of China, No. 5, Yu Shan Road, Qingdao, Shandong Province, 266003, P.R. China.
authors.forename: Bafang
authors.initials: B
authors.lastname: Li
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D003094:Collagen / Q000494:pharmacology / Q000627:therapeutic use*; D048388:Gadiformes; D005753:Gastric Mucosa / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000737:chemistry*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D003094:Collagen; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D019342:Acetic Acid
keywords: acetic acid; collagen peptides; gastric ulcer; protective effect; radical scavenger