title: First molecular evidence of Toxoplasma gondii in opossums (Didelphis virginiana) from Yucatan, Mexico.
abstract: Toxoplasma gondii is an obligate intracellular parasite recognized as a causal agent of toxoplasmosis; zoonotic disease endemic in many countries worldwide, including Mexico. Different species of animals participate in the wild cycle infection, including opossums of the species Didelphis virginiana. Thirteen D. virginiana were captured in Yucatan, Mexico. Detection of T. gondii was achieved by Polymerase Chain Reaction, which determined an infection of 76.9% (10/13) in brains. Positive amplicons were sequenced for analysis, this produced results similar to T. gondii with identity and coverage values of 98% and 96-100%, respectively. This study presents the first molecular evidence of the circulation of T. gondii in D. virginiana from Mexico.
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: M
authors.initials: M
authors.lastname: Torres-Castro
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: H
authors.initials: H
authors.lastname: Noh-Pech
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: R
authors.initials: R
authors.lastname: Puerto-Hernández
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: B
authors.initials: B
authors.lastname: Reyes-Hernández
authors.affiliation: PIMVS, Tumbel Kuxtal A.C., Tixkokob, México.
authors.forename: A
authors.initials: A
authors.lastname: Panti-May
authors.affiliation: Departamento de Zoología, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: S
authors.initials: S
authors.lastname: Hernández-Betancourt
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: A
authors.initials: A
authors.lastname: Yeh-Gorocica
authors.affiliation: Laboratorio de Genética, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: L
authors.initials: L
authors.lastname: González-Herrera
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: J
authors.initials: J
authors.lastname: Zavala-Castro
authors.affiliation: Laboratorio de Enfermedades Emergentes y Re-emergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, México.
authors.forename: F I
authors.initials: FI
authors.lastname: Puerto
keywords: Didelphis virginiana; Mexico; Toxoplasma gondii; Yucatan