title: Optimized combination of dilution and refined QuEChERS to overcome matrix effects of six types of tea for determination eight neonicotinoid insecticides by ultra performance liquid chromatography-electrospray tandem mass spectrometry.
abstract: Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is a primary tool for analysis of low volatility compounds in complex matrices. However, complex matrices, such as different types of tea, complicate analysis through ionization suppression or enhancement. In this study, sample preparation by a refined QuEChERS method combined with a dilution strategy removed almost all matrix effects caused by six types of tea. Tea samples were soaked with water and extracted with acetonitrile, cleaned up with a combination of PVPP (160mg) and GCB (20mg), and dried. Dried extracts were diluted with 20mL acetonitrile/water (15:85, v/v) before analysis by UPLC-MS/MS. The average recoveries of eight neonicotinoid insecticides (dinotefuran, nitenpyram, thiamethoxam, imidacloprid, clothianidin, imidaclothiz, acetamiprid, and thiacloprid) ranged from 66.3 to 108.0% from tea samples spiked at 0.01-0.5mgkg(-1). Relative standard deviations were below 16% for all recovery tests. The limit of quantification ranged from 0.01 to 0.05mgkg(-1).
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China.
authors.forename: Weiting
authors.initials: W
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China.
authors.forename: Xiaosan
authors.initials: X
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China.
authors.forename: Yizheng
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, PR China. Electronic address: hry@ahau.edu.cn.
authors.forename: Ruyan
authors.initials: R
authors.lastname: Hou
authors.affiliation: School of Resource & Environment of Anhui Agricultural University, Key Lab. of Agri-food Safety of Anhui Province, Hefei 230036, PR China. Electronic address: rimaohua@ahau.edu.cn.
authors.forename: Rimao
authors.initials: R
authors.lastname: Hua
mesh_terms: D002853:Chromatography, Liquid; D006146:Guanidines / Q000032:analysis; D007093:Imidazoles / Q000032:analysis; D007306:Insecticides / Q000032:analysis*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000032:analysis; D010078:Oxazines / Q000032:analysis; D011725:Pyridines / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods; D013662:Tea / Q000737:chemistry*; D000077922:Thiamethoxam; D013843:Thiazines / Q000032:analysis; D013844:Thiazoles / Q000032:analysis
chemical_list: D006146:Guanidines; D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D011725:Pyridines; D013662:Tea; D013843:Thiazines; D013844:Thiazoles; C465368:dinotefuran; C480342:clothianidin; C464843:nitenpyram; C082359:imidacloprid; C464485:acetamiprid; D000077922:Thiamethoxam; C417209:thiacloprid; C550087:imidaclothiz
keywords: Dilution; LC–ESI-MS/MS; Matrix effect; Neonicotinoids; Refined QuEChERS; Tea matrix