title: Nanosecond ligand migration and functional protein relaxation in ba3 oxidoreductase: Structures of the B0, B1 and B2 intermediate states.
abstract: Nanosecond time-resolved step-scan FTIR spectroscopy (nTRS (2) -FTIR) has been applied to literally probe the active site of the carbon monoxide (CO)-bound thermophilic ba3 heme-copper oxidoreductase as it executes its function. The nTRS (2) - snapshots of the photolysed heme a3 Fe-CO/CuB species captured a "transition state" whose side chains prevent the photolysed CO to enter the docking cavity. There are three sets of ba3 photoproduct bands of docked CO with different orientation exhibiting different kinetics. The trajectories of the "docked" CO at 2122, 2129 and 2137cm(-1) is referred to in the literature as B2, B1 and B0 intermediate states, respectively. The present data provided direct evidence for the role of water in controlling ligand orientation in an intracavity protein environment.
authors.affiliation: Cyprus University of Technology, Department of Environmental Science and Technology, P.O. Box 50329, 3603 Lemesos, Cyprus.
authors.forename: Antonis
authors.initials: A
authors.lastname: Nicolaides
authors.affiliation: Chemical and Environmental Science Department, University of Limerick, Limerick, Ireland; Materials & Surface Science Institute, University of Limerick, Limerick, Ireland.
authors.forename: Tewfik
authors.initials: T
authors.lastname: Soulimane
authors.affiliation: Cyprus University of Technology, Department of Environmental Science and Technology, P.O. Box 50329, 3603 Lemesos, Cyprus. Electronic address: c.varotsis@cut.ac.cy.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Varotsis
mesh_terms: D002248:Carbon Monoxide / Q000737:chemistry; D003573:Cytochrome b Group / Q000737:chemistry*; D003576:Electron Transport Complex IV / Q000737:chemistry*; D008024:Ligands; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
chemical_list: D003573:Cytochrome b Group; D008024:Ligands; D002248:Carbon Monoxide; C058557:cytochrome ba3; D003576:Electron Transport Complex IV
keywords: Cytochrome c oxidase; Dynamics; Time-resolved step-scan FTIR