title: Characterization, expression analysis and localization pattern of toll-like receptor 1 (tlr1) and toll-like receptor 2 (tlr2) genes in grass carp Ctenopharyngodon idella.
abstract: In this study, the toll-like receptor 1 (tlr1) and toll-like receptor 2 (tlr2) genes of grass carp Ctenopharyngodon idella were cloned and characterized. tlr1 and tlr2 were found to be highly expressed in immune system organs such as spleen, middle kidney and heart kidney. The expression level of tlr1 and tlr2 was found to be up-regulated at the later stage of viral challenge process. Moreover, subcellular localization indicated that Tlr1 and Tlr2 shared similar localization pattern and both of them may locate in the plasma membrane of transfected cells.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: L B
authors.initials: LB
authors.lastname: He
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: L F
authors.initials: LF
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.|Department of Zoology, College of Life Sciences, Kim Il Song University, Pyongyang, Democratic People's Republic of Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Li
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: R
authors.initials: R
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Liao
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Wang
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005393:Fish Diseases / Q000276:immunology / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D012087:Reoviridae; D012088:Reoviridae Infections / Q000276:immunology / Q000378:metabolism; D051194:Toll-Like Receptor 1 / Q000235:genetics / Q000378:metabolism*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2
keywords: grass carp Ctenopharyngodon idellus; grass carp reovirus; immune response; toll-like receptor 1; toll-like receptor 2