title: Effects of maternal low-protein diet on parameters of locomotor activity in a rat model of cerebral palsy.
abstract: Children with cerebral palsy have feeding difficulties that can contribute to undernutrition. The aim of this study was to investigate the effect of early undernutrition on locomotor activity and the expression of the myofibrillar protein MuRF-1 in an experimental model of cerebral palsy (CP). In order to achieve this aim, pregnant rats were divided into two groups according to the diet provided: Normal Protein (NP, n=9) and Low Protein (LP, n=12) groups. After birth, the pups were divided into four groups: Normal Protein Sham (NPS, n=16), Normal Protein Cerebral Palsy (NPCP, n=21), Low Protein Sham (LPS, n=20) and Low Protein Cerebral Palsy (LPCP, n=18) groups. The experimental cerebral palsy protocol consisted of two episodes of anoxia at birth and during the first days of life. Each day, nitrogen flow was used (9l/min during 12min). After nitrogen exposure, sensorimotor restriction was performed 16h per day, from the 2nd to the 28th postnatal day (PND). Locomotor activity was evaluated at 8th, 14th, 17th, 21th and 28th PND. At PND 29, soleus muscles were collected to analyse myofibrillar protein MuRF-1. Our results show that CP animals decreased body weight (p<0.001), which were associated with alterations of various parameters of locomotor activity (p<0.05), compared to their control. Undernourished animals also showed a decrease (p<0.05) in body weight and locomotor activity parameters. Moreover, CP decreased MuRF-1 levels in nourished rats (p=0.015) but not in undernourished rats. In summary, perinatal undernutrition exacerbated the negative effects of cerebral palsy on locomotor activity and muscle atrophy, but it appears not be mediated by changes in MuRF-1 levels.
authors.affiliation: Department of Nutrition, Federal University of Piauí, 64607-670 Picos, Brazil. Electronic address: kassiaoliveira17@yahoo.com.br.
authors.forename: Kássia Oliveira Gomes da
authors.initials: KO
authors.lastname: Silva
authors.affiliation: Department of Physical Therapy, Federal University of Pernambuco, 50670-901 Recife, Brazil. Electronic address: bina_c13@hotmail.com.
authors.forename: Sabrina da Conceição
authors.initials: Sda C
authors.lastname: Pereira
authors.affiliation: Faculty of Applied Sciences, University of Campinas, 13084-970 Campinas, Brazil. Electronic address: mariportovedo@gmail.com.
authors.forename: Mariana
authors.initials: M
authors.lastname: Portovedo
authors.affiliation: Faculty of Applied Sciences, University of Campinas, 13084-970 Campinas, Brazil. Electronic address: marciane.milanski@fca.unicamp.br.
authors.forename: Marciane
authors.initials: M
authors.lastname: Milanski
authors.affiliation: Department of Anatomy, Federal University of Pernambuco, 50670-901 Recife, Brazil. Electronic address: galindo.ligia1@gmail.com.
authors.forename: Lígia Cristina Monteiro
authors.initials: LC
authors.lastname: Galindo
authors.affiliation: Neuro Centre Magendie, INSERM U862, Université Bordeaux 2, 33077 Bordeaux, France. Electronic address: fn.nomore@gmail.com.
authors.forename: Omar
authors.initials: O
authors.lastname: Guzmán-Quevedo
authors.affiliation: Department of Nutrition, Federal University of Pernambuco, 50670-901 Recife, Brazil. Electronic address: manhaesdecastroraul@gmail.com.
authors.forename: Raul
authors.initials: R
authors.lastname: Manhães-de-Castro
authors.affiliation: Department of Nursing, CAV, Federal University of Pernambuco, 55608-680 Vitória de Santo Antão, Brazil. Electronic address: aeltoscano@yahoo.com.br.
authors.forename: Ana Elisa
authors.initials: AE
authors.lastname: Toscano
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000502:physiology; D002547:Cerebral Palsy / Q000209:etiology* / Q000473:pathology; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D004195:Disease Models, Animal; D005260:Female; D008124:Locomotion / Q000502:physiology; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology* / Q000503:physiopathology*; D051381:Rats; D000070584:Tripartite Motif Proteins / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D000070584:Tripartite Motif Proteins; C499086:Trim63 protein, rat; D044767:Ubiquitin-Protein Ligases
keywords: Cerebral palsy; Experimental model; Locomotor activity; Rats; Undernutrition