title: Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells.
abstract: This study was designed to investigate the inhibitory effects of Angelica polysaccharide (AP) on activation of mast cells and its possible molecular mechanism. In our study, we determined the proinflammatory cytokines and allergic mediators in anti-DNP IgE stimulated RBL-2H3 cells and found that AP (50, 100, and 200 μg/mL) significantly decreased the release of histamine, β-hexosaminidase, leukotrienes C4 (LTC4), IL-1, IL-4, TNF-α, IL-6, and human monocyte chemotactic protein-1 (MCP-1/CCL2) (p < 0.05). In addition, Ca(2+) entry was inhibited by treatment with AP. AP also downregulated the protein expressions of p-Fyn, p-Akt, p-P38, IL-4, TNF-α, and NF-κB p65 in both Fyn gene upregulated and normal RBL-2H3 cells (p < 0.05). Collectively, our results showed that AP could inhibit the activation of mast cells via suppressing the releases of proinflammatory cytokines allergic mediators, Gab2/PI3-K/Akt and Fyn/Syk pathways.
authors.affiliation: Department of Dermatology, Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China.
authors.forename: Wei-An
authors.initials: WA
authors.lastname: Mao
authors.affiliation: Department of Graduate, Bengbu Medical College, Bengbu 233000, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Sun
authors.affiliation: Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Mao
authors.affiliation: Department of Dermatology, Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, Liverpool L3 3AF, UK.
authors.forename: Khalid
authors.initials: K
authors.lastname: Rahman
authors.affiliation: Central Laboratory, Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye