title: Interaction of single and multi wall carbon nanotubes with the biological systems: tau protein and PC12 cells as targets.
abstract: Subtle changes in the structure of nanoparticles influence their surface tension and corresponding interaction with cells and proteins. Here, the interaction of the single wall carbon nanotube (SWCNT) and multiwall carbon nanotube (MWCNT) with different surface tension with tau protein was evaluated using a variety of techniques including far and near circular dichroism, fluorescence spectroscopy, dynamic light scattering, Zeta potential, and TEM evaluation. Also the cytotoxicity of SWCNT and MWCNT on the PC12 cell line as a model of nervous system cell line was investigated by the MTT, LDH, acridine orange/ethidium bromide staining, flow cytometry, caspase 3 activity, cell and membrane potential assays. It was observed that SWCNT induced more structural changes of tau protein relative to MWCNT/tau protein interaction. It was also revealed that SWCNT and MWCNT impaired the viability and complexity of PC12 cells in different modes of cytotoxicity. Analysis of cellular outcomes indicated that MWCNT in comparison with SWCNT resulted in induction of necrotic modes of cell death, whereas apoptotic modes of cell death were activated in SWCNT-incubated cells. Together these findings suggest that surface tension may be used to determine how nanoparticle structure affects neurotoxicity and protein conformational changes.
authors.affiliation: Department of nanotechnology, Faculty of Advance Science and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
authors.forename: Hojjat Alizadeh
authors.initials: HA
authors.lastname: Zeinabad
authors.affiliation: Department of nanotechnology, Faculty of Advance Science and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Zarrabian
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
authors.affiliation: Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Alizadeh
authors.affiliation: Department of nanotechnology, Faculty of Advance Science and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Falahati
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D000067493:Dynamic Light Scattering; D008564:Membrane Potentials / Q000187:drug effects; D008954:Models, Biological; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity*; D009420:Nervous System / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D011487:Protein Conformation / Q000187:drug effects; D051381:Rats; D013050:Spectrometry, Fluorescence; D013500:Surface Tension; D016875:tau Proteins / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D016875:tau Proteins; C505410:Casp3 protein, rat; D053148:Caspase 3