title: Characterization of two novel sialyl N-acetyllactosaminyl nucleotides separated from ovine colostrum.
abstract: The milk/colostrum of some mammalian species is known to contain sugar nucleotides including uridine diphosphate (UDP) oligosaccharides in addition to lactose and milk oligosaccharides, but the detailed structures of these UDP oligosaccharides have not so far been clarified. In this study we isolated two UDP-sialyl N-acetyllactosamines from ovine colostrum and characterized them using (1)H-NMR and MALDI-TOFMS spectroscopies. Their structures were found to be Neu5Gc(α2-3)Gal(β1-4)GlcNAcα1-UDP and Neu5Gc(α2-6)Gal(β1-4)GlcNAcα1-UDP.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Sasaki
authors.affiliation: Department of Food Science, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hirayama
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuda
authors.affiliation: Graduate School of Agriculture, Tohoku University, Sendai, Miyagi, 981-8555, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan. urashima@obihiro.ac.jp.
authors.forename: Tadasu
authors.initials: T
authors.lastname: Urashima
authors.affiliation: Intensive Grazing Research Team, National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido, 062-8555, Japan.
authors.forename: Sadaki
authors.initials: S
authors.lastname: Asakuma
mesh_terms: D000818:Animals; D003126:Colostrum / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D012756:Sheep; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000032:analysis* / Q000378:metabolism
chemical_list: D014536:Uridine Diphosphate N-Acetylgalactosamine
keywords: Milk oligosaccharide; Milk sugar nucleotide; Ovine colostrum; UDP oligosaccharide; UDP sialyl N-acetyllactosamine