title: Diarylheptanoids from Alnus viridis ssp. viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa.
abstract: Diarylheptanoids from the barks ofAlnus viridisssp.viridis(green alder) andAlnus glutinosa(black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namelyPseudomonas aeruginosaPAO1 andChromobacterium violaceumCV026. Although all of the investigated compounds negatively influenced the motility ofP. aeruginosaPAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40-70 %. Three of the diarylheptanoids (3, 4, and5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein inC. violaceumCV026, with5being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was shown to significantly decrease the production of acyl homoserine lactones (AHL) inP. aeruginosaPAO1, more specifically, production of the long chain N-(3-oxododecanoyl)-l-HSL. On the other side, four diarylheptanoids (2-5) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of theP. aeruginosaquinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, theirin vitroantiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 µg/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Ilic-Tomic
authors.affiliation: Institute for Biological Research "Sinisa Stankovic", University of Belgrade, Belgrade, Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Sokovic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Vojnovic
authors.affiliation: Institute for Biological Research "Sinisa Stankovic", University of Belgrade, Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Ciric
authors.affiliation: Faculty of Biology, University of Belgrade, Belgrade, Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Veljic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Nikodinovic-Runic
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Novakovic
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism; D029661:Alnus / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002396:Catechols / Q000378:metabolism; D002861:Chromobacterium / Q000187:drug effects* / Q000378:metabolism; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D011710:Pyocyanine / Q000378:metabolism; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: D054742:Acyl-Butyrolactones; D000900:Anti-Bacterial Agents; D002396:Catechols; D036381:Diarylheptanoids; D007211:Indoles; D010936:Plant Extracts; C508777:hirsutenone; D011710:Pyocyanine; C063155:violacein