title: Beneficial role of ascorbic and folic acids antioxidants against thyroxin-induced testicular dysfunction in hyperthyroid rats.
abstract: Thyroid hormones play a fundamental role in the regulation of metabolism of almost all mammalian tissue including the reproductive system. Hyperthyroidism in early life may cause delayed sexual maturation, although physical development is normal and skeletal growth may be accelerated. Hyperthyroidism after puberty influences reproductive functions and increases testosterone level. The aim of this work is to study the effect of induced hyperthyroidism by L-thyroxine sodium administration on the testis of rats and to evaluate the ameliorating role of different antioxidants as ascorbic acid and folic acid on the hyperthyroid state via the assessment of different biochemical markers, histopathological and immunochemical sections. DNA analysis of the D1 deiodinase was performed to determine genetic mutation due to hyperthyroidism. The results showed partially disrupted in the measured biochemical parameters and spermatogenesis in hyperthyroid rats. Post-administration of both folic and ascorbic acids together in hyperthyroid rats showed the best ameliorating effects on the thyroid hormones, testosterone, testicular GGT and ALP, and all oxidative stress markers. There is no genetic mutations that occurred in D1 deiodinase due to hyperthyroidism. These findings were indicated by the proliferating cell nuclear antigen (PCNA) studies of testes.
authors.affiliation: Biochemistry Division, Department of Chemistry, Faculty of Science, Damanhour University, Damanhour, Egypt.|Biochemistry Unit, Chemistry Department, Damanhour, Egypt.|Department of Zoology, Department of Chemistry, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Doha M
authors.initials: DM
authors.lastname: Beltagy
authors.affiliation: Biochemistry Division, Department of Chemistry, Faculty of Science, Damanhour University, Damanhour, Egypt.|Biochemistry Unit, Chemistry Department, Damanhour, Egypt.|Department of Zoology, Department of Chemistry, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Tarek M
authors.initials: TM
authors.lastname: Mohamed
authors.affiliation: Biochemistry Division, Department of Chemistry, Faculty of Science, Damanhour University, Damanhour, Egypt.|Biochemistry Unit, Chemistry Department, Damanhour, Egypt.|Department of Zoology, Department of Chemistry, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: El Said
authors.affiliation: Biochemistry Division, Department of Chemistry, Faculty of Science, Damanhour University, Damanhour, Egypt. toussonehab@yahoo.com.|Biochemistry Unit, Chemistry Department, Damanhour, Egypt. toussonehab@yahoo.com.|Department of Zoology, Department of Chemistry, Faculty of Science, Tanta University, Tanta, Egypt. toussonehab@yahoo.com.
authors.forename: Ehab
authors.initials: E
authors.lastname: Tousson
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D005492:Folic Acid / Q000494:pharmacology*; D006980:Hyperthyroidism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000187:drug effects; D013733:Testicular Diseases / Q000139:chemically induced / Q000188:drug therapy*; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000378:metabolism; D013963:Thyroid Hormones / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D013963:Thyroid Hormones; D013739:Testosterone; D005492:Folic Acid; D001205:Ascorbic Acid; D013974:Thyroxine
keywords: Ascorbic acid; Folic acid; Hyperthyroidism; L-Thyroxine sodium; Testosterone