title: RNAi as a management tool for the western corn rootworm, Diabrotica virgifera virgifera.
abstract: The western corn rootworm (WCR), Diabrotica virgifera virgifera, is the most important pest of corn in the US Corn Belt. Economic estimates indicate that costs of control and yield loss associated with WCR damage exceed $US 1 billion annually. Historically, corn rootworm management has been extremely difficult because of its ability to evolve resistance to both chemical insecticides and cultural control practices. Since 2003, the only novel commercialized developments in rootworm management have been transgenic plants expressing Bt insecticidal proteins. Four transgenic insecticidal proteins are currently registered for rootworm management, and field resistance to proteins from the Cry3 family highlights the importance of developing traits with new modes of action. One of the newest approaches for controlling rootworm pests involves RNA interference (RNAi). This review describes the current understanding of the RNAi mechanisms in WCR and the use of this technology for WCR management. Further, the review addresses ecological risk assessment of RNAi and insect resistance management of RNAi for corn rootworm. © 2016 Society of Chemical Industry.
authors.affiliation: Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Elane
authors.initials: E
authors.lastname: Fishilevich
authors.affiliation: Department of Entomology, University of Nebraska, Lincoln, NE, USA.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Vélez
authors.affiliation: Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Storer
authors.affiliation: Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Huarong
authors.initials: H
authors.lastname: Li
authors.affiliation: Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Bowling
authors.affiliation: Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Murugesan
authors.initials: M
authors.lastname: Rangasamy
authors.affiliation: Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Worden
authors.affiliation: Dow AgroSciences, Indianapolis, IN, USA.
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Narva
authors.affiliation: Entomology and Nematology Department, University of Florida, Gainesville, FL, USA.
authors.forename: Blair D
authors.initials: BD
authors.lastname: Siegfried
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000235:genetics / Q000254:growth & development; D007814:Larva / Q000235:genetics / Q000254:growth & development; D010572:Pest Control, Biological / Q000379:methods*; D034622:RNA Interference
keywords: Diabrotica; RNAi; RNAi risk assessment; insect resistance management; mode of action; western corn rootworm