title: Immunohistochemical expression of p53 and its clinicopathological correlation with modified Anneroth's histological grading system.
abstract: OBJECTIVE
  Oral squamous cell carcinoma (OSCC) is an epithelial neoplasm generally beginning as focal overgrowth of altered stem cells near the basement membrane, moving upward and laterally, replacing the normal epithelium. Histopathological grading has been used for many decades in an attempt to predict the clinical behavior of oral squamous cell carcinoma. In the present study, Forty biopsies were studied for histological grading and p53 expression. The p53 expression was studied in relation to clinical parameters such as age, sex of patient and site of tumors. Relation between histological grade of malignancy and p53 protein expression was analysed. All cases were classified according to Anneroth's histological malignancy grading system (1987).
  
  
  METHODS
  40 cases of OSCC were assessed for clinical parameters, Anneroth's histological grading and immunohistochemically stained with p53 protien.
  
  
  METHODS
  The results obtained were analyzed using Spearman's Co-relation.
  
  
  RESULTS
  The positive expression of p53 was found in 62% of carcinomas studied. Positivity of p53 showed correlation with histological grade of malignancy and with individual parameters like degree of keratinization, nuclear polymorphism, number of mitoses and lymphoplasmacytic infiltration while showed a negative correlation with pattern of invasion.
  
  
  CONCLUSIONS
  Our study showed a significant correlation between parameters of tumor cell population, lymphoplasmacytic infiltration and p53 expression. A significant association between high grade of malignancy and p53 overexpression and insignificant correlation of p53 with age, sex of the patient and site of the tumor was found.
authors.affiliation: Department of Oral Pathology and Microbiology, College of Dental Science and Research Centre, Gujarat University, Ahmedabad, Gujarat, India.
authors.forename: Kajal V
authors.initials: KV
authors.lastname: Dave
authors.affiliation: Department of Oral Pathology and Microbiology, Ahmedabad Dental College and Hospital, Gujarat University, Santej, Gujarat, India.
authors.forename: Monali
authors.initials: M
authors.lastname: Chalishazar
authors.affiliation: Department of ENT, GCS Medical College, Ahmedabad, Gujarat, India.
authors.forename: Vishal R
authors.initials: VR
authors.lastname: Dave
authors.affiliation: Department of Oral Pathology and Microbiology, K M Shah Dental College, Vadodara, Gujarat, India.
authors.forename: Pritam
authors.initials: P
authors.lastname: Panja
authors.affiliation: Department of Oral Pathology and Microbiology, Buddha Institute of Dental Science, Patna, Bihar, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Oral Pathology and Microbiology, College of Dental Science and Research Centre, Gujarat University, Ahmedabad, Gujarat, India.
authors.forename: Tapan G
authors.initials: TG
authors.lastname: Modi
keywords: Malignancy grading system; oral cancer; p53