title: Trypanosoma teixeirae: A new species belonging to the T. cruzi clade causing trypanosomosis in an Australian little red flying fox (Pteropus scapulatus).
abstract: Little is known about the genetic diversity and pathogenicity of trypanosomes in Australian bats. Recently a novel trypanosome species was identified in an adult female little red flying fox (Pteropus scapulatus) with clinical and pathological evidence of trypanosomosis. The present study used morphology and molecular methods to demonstrate that this trypanosome is a distinct species and we propose the name Trypanosoma teixeirae sp. n. Morphological comparison showed that its circulating trypomastigotes were significantly different from those of Trypanosoma pteropi and Trypanosoma hipposideri, two species previously described from Australian bats. Genetic information was not available for T. pteropi and T. hipposideri but phylogenetic analyses at the 18S ribosomal RNA (rRNA) and glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) loci indicated that T. teixeirae sp. n. was genetically distinct and clustered with other bat-derived trypanosome species within the Trypanosoma cruzi clade.
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia; CAPES Foundation, Ministry of Education of Brazil, Brasília, DF, 70040-020, Brazil,. Electronic address: A.DuarteBarbosa@murdoch.edu.au.
authors.forename: Amanda D
authors.initials: AD
authors.lastname: Barbosa
authors.affiliation: Vepalabs, 36 Balaclava Street, Woolloongabba, Queensland 4102, Australia.
authors.forename: John T
authors.initials: JT
authors.lastname: Mackie
authors.affiliation: Australia Zoo Wildlife Hospital, Beerwah, Queensland, 4519, Australia.
authors.forename: Robyn
authors.initials: R
authors.lastname: Stenner
authors.affiliation: Australia Zoo Wildlife Hospital, Beerwah, Queensland, 4519, Australia.
authors.forename: Amber
authors.initials: A
authors.lastname: Gillett
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Irwin
authors.affiliation: School of Veterinary and Life Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia.
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D001315:Australia / Q000453:epidemiology; D002685:Chiroptera / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
keywords: 18S ribosomal RNA (rRNA); Glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH); Little red flying fox (Pteropus scapulatus); Morphology; PCR; Phylogeny; Trypanosoma teixeirae sp. n