title: Comparison of In-House Multiplex Real Time PCR, Diagcor GenoFlow HPV Array Test and INNO-LiPA HPV Genotyping Extra Assays with LCD- Array Kit for Human Papillomavirus Genotyping in Cervical Liquid Based Cytology Specimens and Genital Lesions in Tehran, Iran.
abstract: BACKGROUND
  Human papillomavirus is a major etiologic agent for some human common cancers. Cervical precancer and cancer is the most prevalent dysplasia by HPV genotypes. Various rapid and sensitive methods have been developed into readily HPV genotyping.
  
  
  METHODS
  In the present study, we compared the performance of Real Time PCR, GenoFlow HPV Array, and INNO-LiPA HPV Genotyping Extra Assays with LCD- Array.
  
  
  RESULTS
  From 108 cervical samples, HPV was detected in 33 women (30.55%). Among detected HPV genotypes, HPV 6 and 11 were dominant genotypes. Comparing these methods revealed that for Real Time PCR, Genoflow, and INNO-LiPA in comparison with LCD Array, sensitivity and specificity were 94.2%, 93%; 76.7%, 93%; 64%, 96.5%, respectively. Overall, accuracy and precision of these methods were more than 80% and 90%, respectively.
  
  
  CONCLUSIONS
  It seems that these methods are reliable and suitable for detection and genotyping of HPVs in cervical disorders and other dysplasia associated with human papillomaviruses.
authors.forename: Amir
authors.initials: A
authors.lastname: Sohrabi
authors.forename: Marjan
authors.initials: M
authors.lastname: Rahnamaye-Farzami
authors.forename: Siamak
authors.initials: S
authors.lastname: Mirab-Samiee
authors.forename: Saeed
authors.initials: S
authors.lastname: Mahdavi
authors.forename: Monireh
authors.initials: M
authors.lastname: Babaei
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic