title: Comparative protein profiles of Butea superba tubers under seasonal changes.
abstract: Seasonal changes are major factors affecting environmental conditions which induce multiple stresses in plants, leading to changes in protein relative abundance in the complex cellular plant metabolic pathways. Proteomics was applied to study variations in proteome composition of Butea. superba tubers during winter, summer and rainy season throughout the year using two-dimensional polyacrylamide gel electrophoresis coupled with a nanoflow liquid chromatography coupled to electrospray ionization quadrupole-time-of-flight tandem mass spectrometry. A total of 191 protein spots were identified and also classified into 12 functional groups. The majority of these were mainly involved in carbohydrate and energy metabolism (30.37 %) and defense and stress (18.32 %). The results exhibited the highest numbers of identified proteins in winter-harvested samples. Forty-five differential proteins were found in different seasons, involving important metabolic pathways. Further analysis indicated that changes in the protein levels were due mainly to temperature stress during summer and to water stress during winter, which affected cellular structure, photosynthesis, signal transduction and homeostasis, amino-acid biosynthesis, protein destination and storage, protein biosynthesis and stimulated defense and stress mechanisms involving glycolytic enzymes and relative oxygen species catabolizing enzymes. The proteins with differential relative abundances might induce an altered physiological status within plant tubers for survival. The work provided new insights into the better understanding of the molecular basis of plant proteomes and stress tolerance mechanisms, especially during seasonal changes. The finding suggested proteins that might potentially be used as protein markers in differing seasons in other plants and aid in selecting B. superba tubers with the most suitable medicinal properties in the future.
authors.affiliation: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Chonchanok
authors.initials: C
authors.lastname: Leelahawong
authors.affiliation: Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, 10210, Thailand.
authors.forename: Chantragan
authors.initials: C
authors.lastname: Srisomsap
authors.affiliation: Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Wichai
authors.initials: W
authors.lastname: Cherdshewasart
authors.affiliation: Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, 10210, Thailand.
authors.forename: Daranee
authors.initials: D
authors.lastname: Chokchaichamnankit
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Nawaporn
authors.initials: N
authors.lastname: Vinayavekhin
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. polkit@gmail.com.
authors.forename: Polkit
authors.initials: P
authors.lastname: Sangvanich
mesh_terms: D031290:Butea / Q000378:metabolism*; D004734:Energy Metabolism; D018506:Gene Expression Regulation, Plant; D058977:Molecular Sequence Annotation; D010788:Photosynthesis; D010940:Plant Proteins / Q000378:metabolism*; D035281:Plant Tubers / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D012621:Seasons
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Butea superba; Mass spectrometry; Proteomics; Temperature stress; Two-dimensional gel electrophoresis; Water stress