title: Gibberellin stimulates regrowth after defoliation of sheepgrass (Leymus chinensis) by regulating expression of fructan-related genes.
abstract: Gibberellins (GAs) affect forage growth and development; however, it is largely unknown how GAs regulate the metabolism of fructan (an important polysaccharide reserve in many cereals) and the regrowth of forage plants after defoliation. To explore the mechanism of the responses of defoliated sheepgrass [Leymus chinensis (Trin.) Tzvel] to GA, we sprayed defoliated sheepgrass with GA3 and/or paclobutrazol (PAC; an inhibitor of GA biosynthesis) and analyzed the growth characteristics, carbohydrate contents, and transcript levels of genes related to GA metabolism, GA signal transduction, and fructan metabolism. The results showed that spraying exogenous GA3 onto defoliated sheepgrass promoted leaf and internode elongation, while spraying with PAC inhibited leaf and internode elongation, compared with the control. Spraying GA3 onto defoliated sheepgrass also altered the fructan content by extending the period of fructan utilization. At the transcriptional level, exogenous GA3 increased the transcript levels of genes related to GA metabolism in the sheath. Taken together, our results suggest that exogenous GA3 stimulates the regrowth of defoliated sheepgrass regrowth by regulating GA and fructan-related genes, and by promoting endogenous GA synthesis, fructan metabolism, and signaling.
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Institute of Animal Sciences, the Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.
authors.forename: Linhui
authors.initials: L
authors.lastname: Shao
authors.affiliation: Molecular Genetics Laboratory, Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, NB, E3B 4Z7, Canada.
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, People's Republic of China. liugs@ibcas.ac.cn.
authors.forename: Gongshe
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, People's Republic of China. sychen@ibcas.ac.cn.
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Chen
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000235:genetics; D005630:Fructans / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology*; D006109:Poaceae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013395:Sucrose / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014230:Triazoles / Q000494:pharmacology
chemical_list: D005630:Fructans; D005875:Gibberellins; D012333:RNA, Messenger; D014230:Triazoles; D013395:Sucrose; C053370:paclobutrazol
keywords: Defoliation; Fructan; Gibberellin; Leymus chinensis; Regrowth