title: Skeletal muscle fiber analysis by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometric imaging at high mass and high spatial resolution.
abstract: Skeletal muscles are composed of heterogeneous muscle fibers with various fiber types. These fibers can be classified into different classes based on their different characteristics. MALDI mass spectrometric imaging (MSI) has been applied to study and visualize different metabolomics profiles of different fiber types. Here, skeletal muscles were analyzed by atmospheric pressure scanning microprobe MALDI-MSI at high spatial and high mass resolution.
authors.affiliation: Department of Chemistry, University of Florida, Gainesville, FL, USA.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Tsai
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Giessen, Hessen, Germany.
authors.forename: Dhaka Ram
authors.initials: DR
authors.lastname: Bhandari
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Garrett
authors.affiliation: Department of Aging and Geriatric Research, Institute on Aging, College of Medicine, University of Florida, Gainesville, FL, USA.
authors.forename: Christy S
authors.initials: CS
authors.lastname: Carter
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Giessen, Hessen, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Spengler
authors.affiliation: Department of Chemistry, University of Florida, Gainesville, FL, USA.|Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Yost
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D055432:Metabolomics / Q000379:methods*; D020527:Microscopy, Scanning Probe / Q000379:methods; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: Atmospheric pressure MALDI mass spectrometric imaging; Biomedicine; High mass resolution MS; High spatial resolution MS; Metabolomics; Skeletal muscle fiber type