title: Neuroinvasive influenza virus A(H5N8) in fattening ducks, Hungary, 2015.
abstract: Highly pathogenic avian influenza (HPAI) A virus H5N8 was detected in far east Asian countries during 2014 and emerged in late 2014 in European countries. Hungary reported a HPAI A(H5N8) outbreak during late winter of 2015 at a Pekin duck fattening facility. Epidemiologic monitoring was extended to holdings in neighboring areas and nearby habitats used by wild birds but failed to identify the source of infection. In addition to respiratory symptoms, the affected birds showed lethargy and neuronal signs, including torticollis. Consistent with this finding, influenza A virus antigen was detected in large quantity in the brain. Molecular analysis of the identified strain showed very close genetic relationship (and >99% nucleotide sequence identity) with co-circulating HPAI A(H5N8) strains. A number of unique or rarely detected amino acid changes was detected in the HA (T220I, R512G), the M2 (I39M), the NA (T211I), the NS1 (P85T), and the PB2 (I261V) proteins of the Hungarian strain. Further studies are needed to demonstrate whether any of these mutations can be linked to neuroinvasiveness and neurovirulence in ducks.
authors.affiliation: Institute for Veterinary Medical Research Centre for Agricultural Research, Hungarian Academy of Sciences, H-1143 Budapest, Hungária krt. 21, Hungary. Electronic address: bkrota@hotmail.com.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Andrea Tóthné
authors.initials: AT
authors.lastname: Bistyák
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Thuma
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Gyuris
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Ursu
authors.affiliation: Institute for Veterinary Medical Research Centre for Agricultural Research, Hungarian Academy of Sciences, H-1143 Budapest, Hungária krt. 21, Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Marton
authors.affiliation: Institute for Veterinary Medical Research Centre for Agricultural Research, Hungarian Academy of Sciences, H-1143 Budapest, Hungária krt. 21, Hungary.
authors.forename: Szilvia L
authors.initials: SL
authors.lastname: Farkas
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Eleonóra
authors.initials: E
authors.lastname: Hortobágyi
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Árpád
authors.initials: Á
authors.lastname: Bacsadi
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Dán
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology*; D004196:Disease Outbreaks; D004372:Ducks / Q000821:virology; D015870:Gene Expression; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006814:Hungary / Q000453:epidemiology; D000071225:Influenza A Virus, H5N8 Subtype / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000453:epidemiology* / Q000473:pathology / Q000635:transmission / Q000821:virology; D053609:Lethargy / Q000473:pathology / Q000821:virology; D009154:Mutation; D010802:Phylogeny; D014103:Torticollis / Q000473:pathology / Q000821:virology; D014763:Viral Matrix Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence; D000086082:Viroporin Proteins
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C085820:INS1 protein, influenza virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins; C502893:PB2 protein, influenza virus
keywords: Emergence; H5N8; Highly pathogenic influenza virus; Outbreak; Subtype