title: Integration host factor and LuxR synergistically bind DNA to coactivate quorum-sensing genes in Vibrio harveyi.
abstract: The cell-cell signaling process called quorum sensing allows bacteria to control behaviors in response to changes in population density. In Vibrio harveyi, the master quorum-sensing transcription factor LuxR is a member of the TetR family of transcription factors that both activates and represses genes to coordinate group behaviors, including bioluminescence. Here, we show that integration host factor (IHF) is a key coactivator of the luxCDABE bioluminescence genes that is required together with LuxR for precise timing and expression levels of bioluminescence during quorum sensing. IHF binds to multiple sites in the luxCDABE promoter and bends the DNA in vitro. IHF and LuxR synergistically bind luxCDABE promoter DNA at overlapping, essential binding sites that are required for maximal gene expression in vivo. RNA-seq analysis demonstrated that IHF regulates 300 genes in V. harveyi, and among these are a core set of 19 genes that are also directly bound and regulated by LuxR. We validated these global analyses by demonstrating that both IHF and LuxR are required for transcriptional activation of the osmotic stress response genes betIBA-proXWV. These data suggest that IHF plays an integral role in one mechanism of transcriptional activation by the LuxR-type family of quorum-sensing regulators in vibrios.
authors.affiliation: Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Chaparian
authors.affiliation: Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Olney
authors.affiliation: Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Hustmyer
authors.affiliation: Department of Biology, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Dean A
authors.initials: DA
authors.lastname: Rowe-Magnus
authors.affiliation: Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Julia C
authors.initials: JC
authors.lastname: van Kessel
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D034982:Integration Host Factors / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D053038:Quorum Sensing / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods; D015398:Signal Transduction / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D034982:Integration Host Factors; D008164:Luminescent Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C064778:LuxR autoinducer binding proteins