title: Biomechanical properties of osteoporotic rat femurs after different hormonal treatments: genistein, estradiol, and estradiol/progesterone.
abstract: BACKGROUND
  The purpose of the study is to compare the effects of genistein, estradiol, estradiol/progesterone combination on the bone mineral density and biomechanical properties of ovariectomized rats' bone.
  
  
  METHODS
  50 female adult Sprague-Dawley rats were divided into five groups. Bilaterally ovaeriectomy were performed in all groups except the sham-operated group. Groups were a sham-operated group and a control group (water was given), estradiol treated group (17-β estradiol 0.015 mg/kg per day), genistein treated group (genistein 10 mg/kg per day), and an estradiol/progesterone combination group (17-β estradiol 0.015 mg/kg plus drosperinone 0.028 mg/kg per day). The water or hormones were implemented in relevant groups for eight weeks by orogasthric catheter. The bone mineral density and biomechanical properties of the femur were analyzed.
  
  
  RESULTS
  Genistein, estradiol, and estradiol/progesterone groups increased bone mineral density significantly compared to the control group. In diaphysis and metaphysis bending test, all groups had higher peak load values than the control group. There were statistically significant differences between the estrogen/progesterone group and control group in diaphysis bending with regard to peak load. There were statistically significant differences between the estradiol and control groups in metaphysis bending with regard to peak load. In axial rotation test, all groups had higher peak torque values than the control groups.
  
  
  CONCLUSIONS
  Genistein, estradiol and estrogen/progesterone combination improved the biomechanical properties of the ovariectomized rat bone. Genistein which has less side effects may be considered as an alternative in the treatment of postmenopausal osteoporosis.
authors.affiliation: Department of Orthopaedics and Traumatology, Dicle University, 2100 Diyarbakır, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Azboy
authors.affiliation: Department of Mechanical Engineering, TOBB University of Economics and Technology, 06560 Ankara, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Özkaya
authors.affiliation: Department of Mechanical Engineering, TOBB University of Economics and Technology, 06560 Ankara, Turkey.
authors.forename: Teyfik
authors.initials: T
authors.lastname: Demir
authors.affiliation: Department of Orthopaedics, Göztepe Training and Research Hospital, 34888 İstanbul, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Demirtaş
authors.affiliation: Department of Orthopaedics and Traumatology, Yenimahalle Training and Research Hospital, 0637 Ankara, Turkey.
authors.forename: Arslan
authors.initials: A
authors.lastname: Kağan Arslan
authors.affiliation: Department of Orthopaedics and Traumatology, Dicle University, 2100 Diyarbakır, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Özkul
authors.affiliation: Department of Orthopaedics and Traumatology, Dicle University, 2100 Diyarbakır, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Akcan
authors.affiliation: Department of Orthopaedics and Traumatology, Yenimahalle Training and Research Hospital, 0637 Ankara, Turkey.
authors.forename: Tolga
authors.initials: T
authors.lastname: Tolunay