title: Quantitative detection of pork in commercial meat products by TaqMan® real-time PCR assay targeting the mitochondrial D-loop region.
abstract: The TaqMan® real-time PCR assay using the mitochondrial D-loop region was developed for the quantitative detection of pork in processed meat products. The newly designed primers and probe specifically amplified pork without any cross-reactivity with non-target animal species. The limit of detection of the real-time PCR assay was 0.1pg of heat-treated pork meat and 0.1% (w/w) pork meat in beef and chicken meat mixtures. The quantitative real-time PCR assay was applied to analyze the pork meat content in 22 commercial processed meat products including jerkies, press hams, sausages, hamburger patties and steaks, grilled short rib patties, and nuggets. The developed real-time PCR method was able to detect pork meat in various types of processed meat products that declared the use of pork meat on their label. All processed meat products that declared no use of pork meat showed a negative result in the assay. The method developed in this study showed sensitivity and specificity in the quantification of pork meat in commercial processed meat products.
authors.affiliation: Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Miju
authors.initials: M
authors.lastname: Kim
authors.affiliation: Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Insuk
authors.initials: I
authors.lastname: Yoo
authors.affiliation: Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Shin-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Yeun
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea. Electronic address: hykim@khu.ac.kr.
authors.forename: Hae-Yeong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods; D002415:Cats; D002417:Cattle; D002645:Chickens; D017931:DNA Primers / Q000235:genetics*; D003670:Deer; D004285:Dogs; D004372:Ducks; D005777:Geese; D006041:Goats; D006736:Horses; D007614:Macropodidae; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D008928:Mitochondria / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D000069466:Red Meat / Q000032:analysis; D034561:Sheep, Domestic; D013045:Species Specificity; D020310:Struthioniformes; D013552:Swine; D014421:Turkey
chemical_list: D017931:DNA Primers
keywords: Meat products; Mitochondrial D-loop region; Pork; Quantification; TaqMan real-time PCR