title: Cellobiohydrolases Produce Different Oligosaccharides from Chitosan.
abstract: Chito-oligosaccharides (COSs) are bioactive molecules with interesting characteristics; however, their exploitation is still restricted due to limited amounts accessible with current production strategies. Here we present a strategy for the production of COSs based on hydrolysis of chitosan by using readily available glycosidases. Cellobiohydrolases (EC 3.2.1.91) were compared with chitosanases (EC 3.2.1.132) regarding their ability for COS production, and the resulting fractions were analyzed by MS and NMR. The oligosaccharides had a degree of polymerization between three and six units, and the degree of acetylation (DA) varied depending on the applied enzyme. Different cellobiohydrolases produced COSs with varying DA, and based on comprehensive NMR analysis the preferred cleavage sites of the respective enzymes that show chitosanase and chitinase activity were elucidated. The study reveals the high potential of readily available cellulolytic enzymes besides chitosanases for the production of COSs with distinct structure facilitating access to this bioactive compound class.
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna , Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Gregor
authors.initials: G
authors.lastname: Tegl
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna , Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Öhlknecht
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna , Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Robert
authors.initials: R
authors.lastname: Vielnascher
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna , Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rollett
authors.affiliation: Department of Chemistry, University of Natural Resources and Life Sciences Vienna , Muthgasse 18, 1190 Wien, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofinger-Horvath
authors.affiliation: Department of Chemistry, University of Natural Resources and Life Sciences Vienna , Muthgasse 18, 1190 Wien, Austria.
authors.forename: Paul
authors.initials: P
authors.lastname: Kosma
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna , Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.|ACIB - Austrian Centre of Industrial Biotechnology , Konrad Lorenz Straße 20, 3430 Tulln, Austria.
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D000107:Acetylation; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D058105:Polymerization; D013302:Streptomyces / Q000201:enzymology; D014242:Trichoderma / Q000201:enzymology
chemical_list: D009844:Oligosaccharides; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase; D043366:Cellulose 1,4-beta-Cellobiosidase