title: Assessment of serum thiol/disulfide homeostasis in multiple myeloma patients by a new method.
abstract: OBJECTIVE
  The etiology of multiple myeloma (MM) is not exactly known. This study investigated the role of thiol/disulfide homeostasis in the etiopathogenesis of MM.
  
  
  METHODS
  Some 50 patients with MM (aged 39-84 years) and 50 sex-matched healthy volunteer controls (aged 50-91 years) participated in this study. Venous blood samples were collected, and levels of native thiols, total thiols, and disulfide were measured.
  
  
  RESULTS
  Native and total thiol levels in the control group were determined to be higher than in the study and patient groups (P<0.001). Disulfide levels were found to be higher in the control group than in the study group and higher in newly diagnosed patients than in outpatients who were undergoing treatment (P=0.002). The ratios of thiol levels were found to be similar in both the study and control groups (P>0.05).
  
  
  CONCLUSIONS
  The results of the study show that although there was a decrease in the levels of disulfide, native thiol, and total thiol, the balance of thiol/disulfide was maintained. This is the first study to research the homeostasis of dynamic thiol/disulfide from the perspective of the new method that was used. We hope that this study will encourage and facilitate further studies in this area.
authors.affiliation: a Department of Hematology , Turkiye Yuksek Ihtisas Training and Research Hospital , Ankara , Turkey.
authors.forename: Tekin
authors.initials: T
authors.lastname: Guney
authors.affiliation: b Department of Internal Medicine , Ataturk Training and Research Hospital , Ankara , Turkey.
authors.forename: İlyas Ferit
authors.initials: İF
authors.lastname: Kanat
authors.affiliation: c Department of Biostatistics and Medical Informatics , Ankara Yildirim Beyazit University.
authors.forename: Afra
authors.initials: A
authors.lastname: Alkan
authors.affiliation: d Department of Medical Biochemistry , Ataturk Training and Research Hospital , Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Alisik
authors.affiliation: e Department of Hematology , Ataturk Training and Research Hospital , Ankara , Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Akinci
authors.affiliation: f Department of Geriatrics , Yildirim Beyazıt University , Ankara , Turkey.
authors.forename: Kamile
authors.initials: K
authors.lastname: Silay
authors.affiliation: g Department of Medical Biochemistry , Yildirim Beyazıt University , Ankara , Turkey.
authors.forename: Salim
authors.initials: S
authors.lastname: Neselioglu
authors.affiliation: h Department of Hematology , Yildirim Beyazıt University , Ankara , Turkey.
authors.forename: Imdat
authors.initials: I
authors.lastname: Dilek
authors.affiliation: g Department of Medical Biochemistry , Yildirim Beyazıt University , Ankara , Turkey.
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D004220:Disulfides / Q000378:metabolism*; D005260:Female; D006706:Homeostasis / Q000235:genetics / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: Disulfide; Multiple myeloma; Oxidative stress; Thiol