title: The synthesis, regulation, and functions of sterols in Candida albicans: Well-known but still lots to learn.
abstract: Sterols are the basal components of the membranes of the fungal pathogen Candida albicans, and these membranes determine the susceptibility of C. albicans cells to a variety of stresses, such as ionic, osmotic and oxidative pressures, and treatment with antifungal drugs. The common antifungal azoles in clinical use are targeted to the biosynthesis of ergosterol. In the past years, the synthesis, storage and metabolism of ergosterol in Saccharomyces cerevisiae has been characterized in some detail; however, these processes has not been as well investigated in the human opportunistic pathogen C. albicans. In this review, we summarize the genes involved in ergosterol synthesis and regulation in C. albicans. As well, genes in S. cerevisiae implicated in ergosterol storage and conversions with other lipids are noted, as these provide us clues and directions for the study of the homologous genes in C. albicans. In this report we have particularly focused on the essential roles of ergosterol in the dynamic process of cell biology and its fundamental status in the biological membrane system that includes lipid rafts, lipid droplets, vacuoles and mitochondria. We believe that a thorough understanding of this classic and essential pathway will give us new ideas about drug resistance and morphological switching in C. albicans.
authors.affiliation: a Center for New Drug Research, College of Pharmacy, Second Military Medical University , Shanghai , P.R. China.
authors.forename: Quan-Zhen
authors.initials: QZ
authors.lastname: Lv
authors.affiliation: a Center for New Drug Research, College of Pharmacy, Second Military Medical University , Shanghai , P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yan
authors.affiliation: a Center for New Drug Research, College of Pharmacy, Second Military Medical University , Shanghai , P.R. China.
authors.forename: Yuan-Ying
authors.initials: YY
authors.lastname: Jiang
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001393:Azoles / Q000494:pharmacology; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D025141:Drug Resistance, Fungal; D004875:Ergosterol / Q000096:biosynthesis / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D066292:Lipid Droplets / Q000378:metabolism; D021962:Membrane Microdomains / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008928:Mitochondria / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D013261:Sterols; D004875:Ergosterol
keywords: Candida albicans; biosynthesis; function; regulation; sterols