title: Expression and purification of an engineered human endothelin receptor B in a monomeric form.
abstract: In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.
authors.affiliation: Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia. mishinalexej@gmal.com.
authors.forename: A V
authors.initials: AV
authors.lastname: Mishin
authors.affiliation: Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
authors.forename: A P
authors.initials: AP
authors.lastname: Luginina
authors.affiliation: Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
authors.forename: A P
authors.initials: AP
authors.lastname: Potapenko
authors.affiliation: Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.|Institute of Complex Systems (ICS), ICS-6: Molecular Biophysics, Research Centre Juelich, Juelich, 52425, Germany.
authors.forename: V I
authors.initials: VI
authors.lastname: Borshchevskiy
authors.affiliation: Bridge Institute, Department of Chemistry, University of Southern California, Los Angeles, California, 90089, USA.
authors.forename: V
authors.initials: V
authors.lastname: Katritch
authors.affiliation: The European Molecular Biology Laboratory (EMBL), Grenoble Cedex 9, 38042, France.|CNRS, IBS, Grenoble, 38044, France.
authors.forename: E
authors.initials: E
authors.lastname: Edelweiss
authors.affiliation: Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.
authors.forename: I S
authors.initials: IS
authors.lastname: Okhrimenko
authors.affiliation: Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.|CNRS, IBS, Grenoble, 38044, France.|Universite Grenoble Alpes, IBS, Grenoble, 38044, France.|CEA, IBS, Grenoble, 38044, France.|Institute of Complex Systems (ICS), ICS-6: Molecular Biophysics, Research Centre Juelich, Juelich, 52425, Germany.
authors.forename: V I
authors.initials: VI
authors.lastname: Gordeliy
authors.affiliation: Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia.|Bridge Institute, Department of Chemistry, University of Southern California, Los Angeles, California, 90089, USA.
authors.forename: V G
authors.initials: VG
authors.lastname: Cherezov
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001713:Biphenyl Compounds / Q000737:chemistry; D015153:Blotting, Western; D004151:Dipeptides / Q000737:chemistry; D065128:Endothelin Receptor Antagonists / Q000737:chemistry; D016232:Endothelins / Q000737:chemistry; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D011489:Protein Denaturation; D055550:Protein Stability; D044023:Receptor, Endothelin B / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D061987:Sf9 Cells; D013696:Temperature
chemical_list: D001713:Biphenyl Compounds; D004151:Dipeptides; C000588596:EDNRB protein, human; D065128:Endothelin Receptor Antagonists; D016232:Endothelins; C575746:IFITM5 protein, human; C098287:IRL 2500; D008565:Membrane Proteins; D010446:Peptide Fragments; D044023:Receptor, Endothelin B; D011993:Recombinant Fusion Proteins; C074308:sovateltide