title: Comparison of amino and epoxy functionalized SBA-15 used for carbonic anhydrase immobilization.
abstract: Two functionalized SBA-15 [amine-functionalized SBA-15 (AFS) and epoxy-functionalized SBA-15 (GFS)] with different types of functional groups were synthesized by a hydrothermal process and post functionalized with 3-aminopropyltriethoxysilane (APTES) and 3-glycidyloxypropyltrimethoxysilane (GPTMS), respectively. They were used for the immobilization of carbonic anhydrase (CA). The physicochemical properties of the functionalized SBA-15 were characterized by X-ray powder diffraction (XRD), N2 adsorption-desorption, (13)C, (29)Si solid-state nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). Before and after CA was immobilized on AFS and GFS, the effects of temperature and pH value on the enzyme activity, storage stability, and reusability were investigated using para-nitrophenyl acetate (p-NPA) assay. CA/GFS showed a better performance with respect to storage stability and reusability than CA/AFS. Moreover, the amount of CaCO3 precipitated over CA/AFS was less than that precipitated over CA/GFS, which was almost equal to that precipitated over the free CA. The results indicate that the epoxy group is a more suitable functional group for covalent bonding with CA than the amino group, and GFS is a promising support for CA immobilization.
authors.affiliation: State Key Laboratory of Fine Chemistry, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Xiaoyao
authors.initials: X
authors.lastname: Fei
authors.affiliation: State Key Laboratory of Fine Chemistry, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Shaoyun
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Fine Chemistry, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Dai
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Fine Chemistry, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Fine Chemistry, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address: zalidy5518@vip.sina.com.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000378:metabolism; D011232:Chemical Precipitation; D004356:Drug Storage; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004852:Epoxy Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009596:Nitrophenols; D011437:Propylamines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: C000616917:3-glycidyloxypropyltrimethoxysilane; D000588:Amines; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D009596:Nitrophenols; D011437:Propylamines; C509969:SBA-15; D012821:Silanes; D012822:Silicon Dioxide; C008642:4-nitrophenyl acetate; D002256:Carbonic Anhydrases; C477625:amino-propyl-triethoxysilane
keywords: Carbonic anhydrase; Enzyme biocatalysis; Functionalized SBA-15; Immobilization; Immobilized enzymes