title: Exogenous cofactors for the improvement of bioremoval and biotransformation of sulfamethoxazole by Alcaligenes faecalis.
abstract: Sulfamethoxazole (SMX), an extensively prescribed or administered antibiotic pharmaceutical product, is usually detected in aquatic environments, because of its incomplete metabolism and elimination. This study investigated the effects of exogenous cofactors on the bioremoval and biotransformation of SMX by Alcaligenes faecalis. High concentration (100mg·L(-1)) of exogenous vitamin C (VC), vitamin B6 (VB6) and oxidized glutathione (GSSG) enhanced SMX bioremoval, while the additions of vitamin B2 (VB2) and vitamin B12 (VB12) did not significantly alter the SMX removal efficiency. Globally, cellular growth of A. faecalis and SMX removal both initially increased and then gradually decreased, indicating that SMX bioremoval is likely dependent on the primary biomass activity of A. faecalis. The decreases in the SMX removal efficiency indicated that some metabolites of SMX might be transformed into parent compound at the last stage of incubation. Two transformation products of SMX, N-hydroxy sulfamethoxazole (HO-SMX) and N4-acetyl sulfamethoxazole (Ac-SMX), were identified by a high-performance liquid chromatograph coupled with mass spectrometer. High concentrations of VC, nicotinamide adenine dinucleotide hydrogen (NADH, 7.1mg·L(-1)), and nicotinamide adenine dinucleotide (NAD(+), 6.6mg·L(-1)), and low concentrations of reduced glutathione (GSH, 0.1 and 10mg·L(-1)) and VB2 (1mg·L(-1)) remarkably increased the formation of HO-SMX, while VB12 showed opposite effects on HO-SMX formation. In addition, low concentrations of GSH and NADH enhanced Ac-SMX formation by the addition of A. faecalis, whereas cofactors (VC, VB2, VB12, NAD(+), and GSSG) had no obvious impact on the formation of Ac-SMX compared with the controls. The levels of Ac-SMX were stable when biomass of A. faecalis gradually decreased, indicating the direct effect of biomass on the formation of Ac-SMX by A. faecalis. In sum, these results help us understand the roles played by exogenous cofactors in eliminating SMX by A. faecalis and provide potential strategies for improving SMX biodegradation.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, People's Republic of China.
authors.forename: Yi-Bi
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, People's Republic of China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Life Science, Tianjin Normal University, Tianjin 300072, People's Republic of China.
authors.forename: Qiu-Man
authors.initials: QM
authors.lastname: Xu
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, People's Republic of China. Electronic address: jscheng@tju.edu.cn.
authors.forename: Jing-Sheng
authors.initials: JS
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, People's Republic of China.
authors.forename: Yu-Lu
authors.initials: YL
authors.lastname: Luo
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, People's Republic of China.
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Yuan
mesh_terms: D042481:Alcaligenes faecalis / Q000254:growth & development / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D013420:Sulfamethoxazole / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D014874:Water Pollutants, Chemical; D013420:Sulfamethoxazole
keywords: Antibiotics; Biotransformation; Cofactor; Removal; Sulfamethoxazole