title: Winogradskyella sediminis sp. nov., isolated from marine sediment.
abstract: A Gram-stain-negative, rod-shaped, yellow-pigmented, gliding bacterial strain, designated S5-23-3T, was isolated from a sediment sample of the Yellow Sea in China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S5-23-3T was related to the genus Winogradskyella and had highest 16S rRNA gene sequence similarities with Winogradskyella arenosi JCM 15527T (97.6 %), Winogradskyella rapida CECT 7392T (97.4 %) and Winogradskyella undariae KCTC 32261T (97.2 %). The predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH. Strain S5-23-3T contained MK-6 as the predominant menaquinone. The polar lipid profile contained phosphatidylethanolamine, two aminolipids, one aminoglycolipid, one aminophospholipid, one unidentified phospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain S5-23-3T was 36.1 mol%. Combined data from phenotypic, chemotaxonomic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain S5-23-3T is a representative of a novel species of the genus Winogradskyella, for which the name Winogradskyellasediminis sp. nov. (type strain S5-23-3T=LMG 28075T=DSM 28134T) is proposed.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PR China.
authors.forename: De-Chao
authors.initials: DC
authors.lastname: Zhang
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PR China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PR China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Weber
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
authors.forename: Rosa
authors.initials: R
authors.lastname: Margesin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6