title: A compact light-sheet microscope for the study of the mammalian central nervous system.
abstract: Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca(2+) signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
authors.affiliation: SUPA, School of Physics &Astronomy, University of St Andrews, St Andrews, KY16 9SS, United Kingdom.
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford, OX1 3QT, United Kingdom.
authors.forename: Peter
authors.initials: P
authors.lastname: Haslehurst
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford, OX1 3QT, United Kingdom.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Scott
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford, OX1 3QT, United Kingdom.
authors.forename: Nigel
authors.initials: N
authors.lastname: Emptage
authors.affiliation: SUPA, School of Physics &Astronomy, University of St Andrews, St Andrews, KY16 9SS, United Kingdom.
authors.forename: Kishan
authors.initials: K
authors.lastname: Dholakia
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000166:cytology / Q000502:physiology; D020013:Calcium Signaling; D002490:Central Nervous System / Q000033:anatomy & histology* / Q000166:cytology / Q000502:physiology; D004867:Equipment Design; D005456:Fluorescent Dyes; D007834:Lasers; D008297:Male; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D010782:Photolysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005456:Fluorescent Dyes