title: Eu/Tb codoped spindle-shaped fluorinated hydroxyapatite nanoparticles for dual-color cell imaging.
abstract: Lanthanide doped fluorinated hydroxyapatite (FAp) nanoparticles are promising cell imaging nanomaterials but they are excited at wavelengths which do not match the light sources usually found in a commercial confocal laser scanning microscope (CLSM). In this work, we have successfully prepared spindle-shaped Eu/Tb codoped FAp nanoparticles by a hydrothermal method. Compared with single Eu doped FAp, Eu/Tb codoped FAp can be excited by a 488 nm laser, and exhibit both green and red light emission. By changing the amounts of Eu and Tb peaks, the emission in the green region (500-580 nm) can be decreased to the benefit of the emission in the red region (580-720 nm), thus reaching a balanced dual color emission. Using MC3T3-E1 cells co-cultured with Eu/Tb codoped FAp nanoparticles, it is observed that the nanoparticles are cytocompatible even at a concentration as high as 800 μg ml(-1). The Eu/Tb codoped FAp nanoparticles are located in the cytoplasm and can be monitored by dual color-green and red imaging with a single excitation light at 488 nm. At a concentration of 200 μg ml(-1), the cytoplasm is saturated in 8 hours, and Eu/Tb codoped FAp nanoparticles retain their fluorescence for at least 3 days. The cytocompatible Eu/Tb codoped FAp nanoparticles with unique dual color emission will be of great use for cell and tissue imaging.
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Baojin
authors.initials: B
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Jichuan
authors.initials: J
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Sang
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Haibing
authors.initials: H
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Huaidong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: NanoQAM, Department of Chemistry, Quebec Center for Functional Materials, UQAM Succ Centre Ville, CP8888, Montreal, Quebec H3C 3P8, Canada. claverie.jerome@uqam.ca.
authors.forename: Jerome
authors.initials: J
authors.lastname: Claverie
authors.affiliation: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China. hdjiang@sdu.edu.cn hongliu@sdu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D003116:Color; D017886:Durapatite / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D008027:Light; D051379:Mice; D053758:Nanoparticles; D061848:Optical Imaging
chemical_list: D005063:Europium; D017886:Durapatite