title: De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation.
abstract: Cold stimulation of Bailinggu's mycelia is the main factor that triggers primordia initiation for successful production of fruiting bodies under commercial cultivation. Yet, the molecular-level mechanisms involved in mycelia response to cold stimulation are still unclear. Here, we performed comparative transcriptomic analysis using RNA-Seq technology to better understand the gene expression regulation during different temporal stages of cold stimulation in Bailinggu. A total of 21,558 Bailinggu mycelia unigenes were de novo assembled and annotated from four libraries (control at 25 °C, plus cold stimulation treatments at -3 °C for a duration of 1-2 days, 5-6 days, and 9-10 days). GO and KEGG pathway analysis indicated that functional groups of differentially expressed unigenes associated with cell wall and membrane stabilization, calcium signaling and mitogen-activated protein kinases (MAPK) pathways, and soluble sugars and protein biosynthesis and metabolism pathways play a vital role in Bailinggu's response to cold stimulation. Six hundred and seven potential EST-based SSRs loci were identified in these unigenes, and 100 EST-SSR primers were randomly selected for validation. The overall polymorphism rate was 92% by using 10 wild strains of Bailinggu. Therefore, these results can serve as a valuable resource for a better understanding of the molecular mechanisms associated with Bailinggu's response to cold stimulation.
authors.affiliation: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. yongpingfu81@126.com.|Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99163, USA. yongpingfu81@126.com.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Fu
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. liangyuan@big.ac.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. daiyueting18@163.com.
authors.forename: Yue-Ting
authors.initials: YT
authors.lastname: Dai
authors.affiliation: China National GeneBank, Environmental Genomics, BGI, Shenzhen 518083, China. yangchentao@genomics.cn.
authors.forename: Chen-Tao
authors.initials: CT
authors.lastname: Yang
authors.affiliation: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. duanmingzheng@hotmail.com.
authors.forename: Ming-Zheng
authors.initials: MZ
authors.lastname: Duan
authors.affiliation: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. zzo1018@126.com.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. husn@big.ac.cn.
authors.forename: Song-Nian
authors.initials: SN
authors.lastname: Hu
authors.affiliation: Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99163, USA. zhiwu.zhang@wsu.edu.
authors.forename: Zhi-Wu
authors.initials: ZW
authors.lastname: Zhang
authors.affiliation: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. yuli966@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D020013:Calcium Signaling / Q000235:genetics; D003080:Cold Temperature; D058639:Cold-Shock Response / Q000235:genetics*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D020929:Mitogen-Activated Protein Kinase Kinases / Q000096:biosynthesis / Q000235:genetics; D025282:Mycelium / Q000235:genetics / Q000254:growth & development; D020076:Pleurotus / Q000235:genetics* / Q000254:growth & development; D014176:Protein Biosynthesis / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D020929:Mitogen-Activated Protein Kinase Kinases
keywords: Bailinggu; EST-SSR; cold stress; comparative transcriptomic analysis; qPCR-PCR