title: Synergistic Effects of Electroacupuncture and Mesenchymal Stem Cells on Intestinal Ischemia/Reperfusion Injury in Rats.
abstract: Electroacupuncture (EA) and transplantation of bone marrow mesenchymal stem cells (MSCs) are both promising therapeutic applications for intestinal disorders. The current study examined their combined effect on rat intestinal ischemia/reperfusion (I/R) injury and the possible mechanism. Five groups were performed: con group (shame operation),I/R group (model group), MSC group (I/R + MSC), EA group (I/R + EA), and combined group (I/R + MSC + EA). Intestinal histological damage, crypt cell proliferation degree, mucosal cytokines expression, and levels of inflammation factors were studied for each group. Compared with the I/R group, crypt cell proliferation index and mucosal mRNA concentration of SDF-1, CXCR4, EGF, EGFR in MSC group and EA group were significantly increased, with mucosal NF-кBp65 and serum inflammation factor (TNF-α, IL-6) levels significantly decreased. Above all of these indicators except NF-кBp65 were improved more notably in combined group than the other two treatment groups. Chiu's score was only ameliorated remarkably in the combined group. The combined treatment of MSC transplantion and electroacupuncture could protect intestinal mucosal barrier from I/R injury.
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Han Zhong Road, Nanjing, 210029, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Acupuncture and Rehabilitation Department, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Han Zhong Road, Nanjing, 210029, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Han Zhong Road, Nanjing, 210029, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Han Zhong Road, Nanjing, 210029, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Han Zhong Road, Nanjing, 210029, China.
authors.forename: Mingqi
authors.initials: M
authors.lastname: Chen
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Han Zhong Road, Nanjing, 210029, China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Han Zhong Road, Nanjing, 210029, China. wangxing1964@163.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy / Q000379:methods; D015671:Electroacupuncture; D007249:Inflammation / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007422:Intestines / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D015427:Reperfusion Injury / Q000628:therapy*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D051996:Transcription Factor RelA
keywords: electroacupuncture; ischemia/reperfusion injury; mesenchymal stem cells; point ST 36 (Zusanli)