title: Recent advances in marine N-cycle studies using15N labeling methods.
abstract: 15N enriched compounds such as ammonium and nitrate, as well as15-15N2gas are invaluable tools in marine N-cycle research.15N stable isotope approaches allow researchers to delve into the often complex world of N-transformations and trace microbially mediated processes such as nitrification, denitrification, anammox and N-fixation. While15N stable isotope approaches are well established, experimental approaches which take advantage of them are constantly evolving. Here we summarize recent advances in methodology, including in the direct application of15N stable isotopes themselves, improved experimental design and the use of15N stable isotopes in single cell studies. Furthermore, we discuss how these advances have led to new insights into marine N-cycling, particularly in the fields of nitrification and N-fixation.
authors.affiliation: Max Planck Institute for Marine Microbiology, Bremen, Germany. Electronic address: hmarchan@mpi-bremen.de.
authors.forename: Hannah K
authors.initials: HK
authors.lastname: Marchant
authors.affiliation: Max Planck Institute for Marine Microbiology, Bremen, Germany.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Mohr
authors.affiliation: Max Planck Institute for Marine Microbiology, Bremen, Germany.
authors.forename: Marcel Mm
authors.initials: MM
authors.lastname: Kuypers
mesh_terms: D007553:Isotope Labeling / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism*; D058458:Nitrogen Cycle / Q000502:physiology*; D009587:Nitrogen Isotopes / Q000032:analysis*; D012623:Seawater / Q000737:chemistry*
chemical_list: D009587:Nitrogen Isotopes; D009584:Nitrogen