title: Urinary proteomic pattern in female stress urinary incontinence: a pilot study.
abstract: OBJECTIVE
  Previous studies aiming to identify specific pre-defined urine protein biomarkers for stress urinary incontinence (SUI) have not identified clinically important differences. The hypothesis of our study was that the global distribution of urinary proteins, the proteome, differs between women with and those without SUI.
  
  
  METHODS
  In this age-matched case-control study, we compared the urinary proteome of 20 women with SUI and 20 controls. Proteins were identified by applying high-performance liquid chromatography separation and tandem mass spectrometry detection. Data analysis was performed using Mascot 2.4.1 embedded in ProteinScape 3.1.
  
  
  RESULTS
  We identified 828 different proteins. The concentration of six of those showed a significant difference between urine samples of SUI patients and those of controls (q value < 0.25). Four proteins showed a higher abundance in SUI samples compared with controls: plasma serine protease inhibitor (logFC 1.11), leucine-rich alpha-2-glycoprotein (logFC 3.91), lysosomal alpha-glucosidase (logFC 1.24), and peptidyl-prolyl cis- trans isomerase A (logFC 1.96). We identified two proteins in lower abundance in SUI samples compared with controls: uromodulin (logFC -4.87) and TALPID3 (logFC -1.99).
  
  
  CONCLUSIONS
  Overexpression of plasma serine protease inhibitor, leucine-rich alpha-2-glycoprotein, lysosomal alpha-glucosidase, and peptidyl-prolyl cis- trans isomerase A, and lower expression of uromodulin and TALPID3, in urine may be associated with female SUI.
authors.affiliation: Clinical Division of General Gynecology and Gynecological Oncology, Department of Obstetrics and Gynecology, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
authors.forename: Marianne
authors.initials: M
authors.lastname: Koch
authors.affiliation: Core Facility Proteomics, Clinical Institute of Laboratory Medicine, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
authors.forename: Goran
authors.initials: G
authors.lastname: Mitulovic
authors.affiliation: Clinical Division of General Gynecology and Gynecological Oncology, Department of Obstetrics and Gynecology, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
authors.forename: Engelbert
authors.initials: E
authors.lastname: Hanzal
authors.affiliation: Clinical Division of General Gynecology and Gynecological Oncology, Department of Obstetrics and Gynecology, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Umek
authors.affiliation: Core Facility Proteomics, Clinical Institute of Laboratory Medicine, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
authors.forename: Sonja
authors.initials: S
authors.lastname: Seyfert
authors.affiliation: Science Consult Thomas Mohr KG, Guntramsdorf, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mohr
authors.affiliation: Clinical Division of General Gynecology and Gynecological Oncology, Department of Obstetrics and Gynecology, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Koelbl
authors.affiliation: Clinical Division of General Gynecology and Gynecological Oncology, Department of Obstetrics and Gynecology, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria. suiproteomics@gmail.com.
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Laterza
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D010865:Pilot Projects; D011507:Proteinuria / Q000378:metabolism*; D040901:Proteomics; D057057:Serine Proteases / Q000097:blood; D014550:Urinary Incontinence, Stress / Q000652:urine*
chemical_list: D015415:Biomarkers; D057057:Serine Proteases
keywords: Leucine- rich alpha-2-glycoprotein; Lysosomal alpha-glucosidase; Plasma serine protease inhibitor; Stress urinary incontinence; TALPID3; Urinary proteome; Uromodulin