title: One step N-glycosylation by filamentous fungi biofilm in bioreactor of a new phosphodiesterase-3 inhibitor tetrazole.
abstract: An efficient and rapid process for N-glycosylation of 5-(1-(3-fluorophenyl)-1H-pyrazol-4-yl)-2H-tetrazole-LQFM 021 (1), a new synthetic derivative of pyrazole with phosphodiesterase-3 (PDE-3) inhibitory action, vasorelaxant activity and low toxicity catalyzed by filamentous fungi biofilm in bioreactor was successfully developed. A maximum N-glycosyl yield of 68% was obtained with Cunninghamella echinulata ATCC 9244 biofilm in bioreactor with conditions of 25mgml(-1) of 1 in PDSM medium at 28°C for 96h. After extraction with ethyl acetate, the derivative was identified by Ultrahigh Resolution Mass Spectrometry and (1)H-(13)C HSQC/HMBC.
authors.affiliation: Laboratório de Bioconversão, Instituto de Química, Universidade Federal de Goiás, P.O. Box 131, Goiânia, GO, Brazil.
authors.forename: Paula L
authors.initials: PL
authors.lastname: de Melo Souza
authors.affiliation: Laboratório de Bioconversão, Instituto de Química, Universidade Federal de Goiás, P.O. Box 131, Goiânia, GO, Brazil.
authors.forename: Evilanna L
authors.initials: EL
authors.lastname: Arruda
authors.affiliation: Laboratório de Química Farmacêutica, Faculdade de Farmácia, Instituto de Química, Universidade Federal de Goiás, P.O. Box 131, Goiânia, GO, Brazil.
authors.forename: Francine
authors.initials: F
authors.lastname: Pazini
authors.affiliation: Laboratório de Química Farmacêutica, Faculdade de Farmácia, Instituto de Química, Universidade Federal de Goiás, P.O. Box 131, Goiânia, GO, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Menegatti
authors.affiliation: Laboratório de Cromatografia e Espectrometria de Massas, Instituto de Química, Universidade Federal de Goiás, P.O. Box 131, Goiânia, GO, Brazil.
authors.forename: Boniek G
authors.initials: BG
authors.lastname: Vaz
authors.affiliation: Laboratório de Ressonância Magnética Nuclear, Instituto de Química, Universidade Federal de Goiás, P.O. Box 131, Goiânia, GO, Brazil.
authors.forename: Luciano M
authors.initials: LM
authors.lastname: Lião
authors.affiliation: Laboratório de Bioconversão, Instituto de Química, Universidade Federal de Goiás, P.O. Box 131, Goiânia, GO, Brazil. Electronic address: valeriaoliveira@ufg.br.
authors.forename: Valéria
authors.initials: V
authors.lastname: de Oliveira
mesh_terms: D018441:Biofilms; D019149:Bioreactors; D020093:Cunninghamella / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006031:Glycosylation; D015394:Molecular Structure; D058987:Phosphodiesterase 3 Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013777:Tetrazoles / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D058987:Phosphodiesterase 3 Inhibitors; D013777:Tetrazoles; C045574:1H-tetrazole; D010727:Phosphoric Diester Hydrolases
keywords: Biofilm bioreactor; Cunninghamella; Glycosylation; LQFM 021; Phosphodiesterase-3 inhibitor