title: Analysis of the Proteins Secreted from the Oryza meyeriana Suspension-Cultured Cells Induced by Xanthomonas oryzae pv. oryzae.
abstract: Oryza meyeriana, a wild species of rice from China, shows high resistance to Xanthomonas oryzae pv. oryzae (Xoo), the cause of rice bacterial blight, one of the most serious rice pathogens. To better understand the resistance mechanism, a proteomic study was conducted to identify changes in the proteins secreted in embryo cell suspension cultures in response to Xoo. After two-dimensional difference gel electrophoresis (2D-DIGE), 72 differentially expressed protein spots corresponding to 34 proteins were identified by Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry. Of the 34 proteins, 10 were up regulated and 24 down regulated. The secreted proteins identified were predicted to be involved in various biological processes, including signal transduction, defense, ROS and cell wall modification. 77% of the 34 proteins were predicted to have a signal peptide by Signal P. Quantitative Real-Time PCR showed that transcript levels of 14 secreted proteins were not well correlated with secreted protein levels. Peroxidase activity was up regulated in both O. meyriana and susceptible rice but was about three times higher in O. meyeriana. This suggests that peroxidases may play an important role in the early response to Xoo in O. meyeriana. These results not only provide a better understanding of the resistance mechanism of O. meyeriana, but have implications for studies of the interactions between other plants and their pathogens.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China.|College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China.|College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China.|College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China.
authors.forename: Chulang
authors.initials: C
authors.lastname: Yu
authors.affiliation: College of Plant Protection, Hunan Agricultural University, Changshao, 410128, China.
authors.forename: XianPing
authors.initials: X
authors.lastname: Fang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Deng
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China.
authors.forename: Chengqi
authors.initials: C
authors.lastname: Yan
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China.|College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
mesh_terms: D002473:Cell Wall; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D018384:Oxidative Stress; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics*; D020543:Proteome; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D057825:Two-Dimensional Difference Gel Electrophoresis; D014974:Xanthomonas
chemical_list: D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species