title: Elongator Protein 3 (Elp3) stabilizes Snail1 and regulates neural crest migration in Xenopus.
abstract: Elongator protein 3 (Elp3) is the enzymatic unit of the elongator protein complex, a histone acetyltransferase complex involved in transcriptional elongation. It has long been shown to play an important role in cell migration; however, the underlying mechanism is unknown. Here, we showed that Elp3 is expressed in pre-migratory and migrating neural crest cells in Xenopus embryos, and knockdown of Elp3 inhibited neural crest cell migration. Interestingly, Elp3 binds Snail1 through its zinc-finger domain and inhibits its ubiquitination by β-Trcp without interfering with the Snail1/Trcp interaction. We showed evidence that Elp3-mediated stabilization of Snail1 was likely involved in the activation of N-cadherin in neural crest cells to regulate their migratory ability. Our findings provide a new mechanism for the function of Elp3 in cell migration through stabilizing Snail1, a master regulator of cell motility.
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.|Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, 650203, China.
authors.forename: Xiangcai
authors.initials: X
authors.lastname: Yang
authors.affiliation: Center of Molecular Diagnostics, The Affiliated Hospital of KMUST, Medical School, Kunming University of Science and Technology, Kunming 650032, China.
authors.forename: Jiejing
authors.initials: J
authors.lastname: Li
authors.affiliation: China Tobacco Yunnan Industrial Co., Ltd., Kunming, 650024, China.
authors.forename: Wanli
authors.initials: W
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
authors.forename: Chaocui
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Mao
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement; D018507:Gene Expression Regulation, Developmental; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D009432:Neural Crest / Q000196:embryology*; D000071250:Snail Family Transcription Factors / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism; D044844:beta-Transducin Repeat-Containing Proteins / Q000378:metabolism
chemical_list: D015820:Cadherins; D006657:Histones; D000071250:Snail Family Transcription Factors; D029867:Xenopus Proteins; D044844:beta-Transducin Repeat-Containing Proteins; C000629272:Elp3 protein, Xenopus; D051548:Histone Acetyltransferases