title: The accelerating effect of chitosan-silica hybrid dressing materials on the early phase of wound healing.
abstract: Commercialized dressing materials with or without silver have played a passive role in early-phase wound healing, protecting the skin defects from infections, absorbing exudate, and preventing dehydration. Chitosan (CTS)-based sponges have been developed in pure or hybrid forms for accelerating wound healing, but their wound-healing capabilities have not been extensively compared with widely used commercial dressing materials, providing limited information in a practical aspect. In this study, we have developed CTS-silica (CTS-Si) hybrid sponges with water absorption, flexibility, and mechanical behavior similar to those of CTS sponges. In vitro and in vivo tests were performed to compare the CTS-Si sponges with three commercial dressing materials [gauze, polyurethane (PU), and silver-containing hydrofiber (HF-Ag)] in addition to CTS sponges. Both in vitro and in vivo tests showed that CTS-Si sponges promoted fibroblast proliferation, leading to accelerated collagen synthesis, whereas the CTS sponges did not exhibit significant differences in fibroblast proliferation and collagen synthesis from gauze, PU, and HF-Ag sponges. In case of CTS-Si, the inflammatory cells were actively recruited to the wound by the influence of the released silicon ions from CTS-Si sponges, which, in return, led to an enhanced secretion of growth factors, particularly TGF-β during the early stage. The higher level of TGF-β likely improved the proliferation of fibroblasts, and as a result, collagen synthesis by fibroblasts became remarkably productive, thereby increasing collagen density at the wound site. Therefore, the CTS-Si hybrid sponges have considerable potential as a wound-dressing material for accelerating wound healing. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1828-1839, 2017.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Seoul National University Boramae Hospital, Dongjak-Gu, Seoul, Republic of Korea.
authors.forename: Ji-Ung
authors.initials: JU
authors.lastname: Park
authors.affiliation: Liquid Processing & Casting Technology R&D Group, Korea Institute of Industrial Technology, Incheon, Republic of Korea.
authors.forename: Hyun-Do
authors.initials: HD
authors.lastname: Jung
authors.affiliation: Department of Materials Science and Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Eun-Ho
authors.initials: EH
authors.lastname: Song
authors.affiliation: Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Jongno-Gu, Seoul, Republic of Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Choi
authors.affiliation: Department of Materials Science and Engineering, Seoul National University, Seoul, Republic of Korea.|Biomedical Implant Convergence Research Center, Advanced Institutes of Convergence Technology, Suwon, Republic of Korea.
authors.forename: Hyoun-Ee
authors.initials: HE
authors.lastname: Kim
authors.affiliation: Biomedical Implant Convergence Research Center, Advanced Institutes of Convergence Technology, Suwon, Republic of Korea.
authors.forename: Juha
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Jongno-Gu, Seoul, Republic of Korea.
authors.forename: Sukwha
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D001458:Bandages; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D012822:Silicon Dioxide; D048271:Chitosan
keywords: chitosan-silica; collagen synthesis; hybrid sponges; wound healing; wound-dressing materials