title: Fluorescent carbon nanoparticles: A low-temperature trypsin-assisted preparation and Fe(3+) sensing.
abstract: In recent years, extensive researches are focused on the fluorescent carbon nanoparticles (CNPs) due to their excellent photochemical, biocompatible and water-soluble properties. However, these synthesis methods are generally suffered from tedious processes. In this paper, fluorescent carbon nanoparticles are synthesized by a facile, one-pot, low-temperature method with trypsin and dopamine as precursors. The synthesis process avoids any heating operation and organic solvent, which provides a "green" and effective preparation route. The obtained CNPs exhibit excellent water-solubility, salt-tolerance and photostability. Based on the synergistic action of the inner filter effect and static quenching mechanism, the CNPs are exploited as a "turn-off" fluorescence sensor for sensitive and selective detection of Fe(3+) ions. The probe shows a wide linear range from 0.1 to 500 μM, with a limit of detection of 30 nM. Furthermore, the as-fabricated fluorescent sensing system is successfully applied to the analysis of Fe(3+) in biological samples such as human urine and serum samples with satisfactory recoveries (92.8-113.3%).
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address: chenyl@lzu.edu.cn.
authors.forename: Yonglei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yangxia
authors.initials: Y
authors.lastname: Han
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D002244:Carbon / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D002244:Carbon; D014357:Trypsin
keywords: Carbon nanoparticles; Dopamine; Fluorescent sensor; Low-temperature synthesis; Trypsin