title: Porous Scaffold Seeding and Chondrogenic Differentiation of BMSC-seeded Scaffolds.
abstract: Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects (Borneset al., 2014). BMSCs can be seeded within porous biomaterial scaffolds that support three-dimensional cell organization, chondrogenic differentiation and extracellular matrix deposition for the creation of engineered cartilage. This protocol describes our defined methods for isolation and expansion of human and ovine BMSCs, seeding of BMSCs within porous scaffolds andin vitrochondrogenic differentiation (Adesidaet al., 2012; Borneset al., 2015).
authors.affiliation: Laboratory of Stem Cell Biology and Orthopaedic Tissue Engineering, Divisions of Orthopaedic Surgery and Surgical Research, Department of Surgery, University of Alberta, Edmonton, Canada.
authors.forename: Troy D
authors.initials: TD
authors.lastname: Bornes
authors.affiliation: Laboratory of Stem Cell Biology and Orthopaedic Tissue Engineering, Divisions of Orthopaedic Surgery and Surgical Research, Department of Surgery, University of Alberta, Edmonton, Canada.
authors.forename: Nadr M
authors.initials: NM
authors.lastname: Jomha
authors.affiliation: Laboratory of Stem Cell Biology and Orthopaedic Tissue Engineering, Divisions of Orthopaedic Surgery and Surgical Research, Department of Surgery, University of Alberta, Edmonton, Canada.
authors.forename: Aillette
authors.initials: A
authors.lastname: Mulet-Sierra
authors.affiliation: Laboratory of Stem Cell Biology and Orthopaedic Tissue Engineering, Divisions of Orthopaedic Surgery and Surgical Research, Department of Surgery, University of Alberta, Edmonton, Canada.
authors.forename: Adetola B
authors.initials: AB
authors.lastname: Adesida