title: Chiroplasmonic Assemblies of Gold Nanoparticles for Ultrasensitive Detection of 8-Hydroxy-2'-deoxyguanosine in Human Serum Sample.
abstract: Gold nanoparticles (AuNPs) have been extensively explored to be used in analytical methods such as electrochemical, colorimetric methods, and so on. However, only a few methods have been reported by using chirality of AuNPs although their chiral assembly has been studied extensively and circular dichroism (CD) spectroscopy is also a simple and sensitive analytical method. In this paper, sensitive CD spectroscopy method has been explored for detection of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a well-known biomarker for oxidative DNA damage, based on DNA-induced chiroplasmonic assemblies of AuNPs. First, 8-OHdG aptamer hybridized with its complementary sequence that modified with AuNPs based on precision matched bases. DNA-modified AuNPs were assembled into AuNPs dimers by 8-OHdG aptamer, which displayed strong chiroptical activity. Subsequently, in the presence of 8-OHdG, the high specific recognition and affinity constants of aptamer and 8-OHdG destroyed the hybrid of aptamer and its complementary sequence; as a result, AuNPs dimers were destroyed and showed low CD signal. The CD intensity was in log-linear correlation with the concentration of 8-OHdG ranging from 0.05 to 2 nM, with a correlation coefficient of 0.9951 and a detection limit of 33 pM (S/N = 3). The method has been successfully applied in a complex matrix such as human serum samples. The recoveries were from 92.5% to 107% and the relative standard derivations were in the range of 4.89% ∼ 7.27%, indicating that the method had good accuracy and high precision. Therefore, these results indicated that the proposed CD method was simple and reliable, which held great potential for clinical examinations.
authors.affiliation: Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing, 211189, China.
authors.forename: Yuanjian
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology, Henan University of Technology , Zhengzhou, 450001, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.affiliation: Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing, 211189, China.
authors.forename: Linqun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing, 211189, China.
authors.forename: Yuanjian
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing, 211189, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing, 211189, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Yin
authors.affiliation: Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing, 211189, China.
authors.forename: Yuepu
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing, 211189, China.
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D019281:Dimerization; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine