title: A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films.
abstract: TfCut2 from Thermobifida fusca KW3 and the metagenome-derived LC-cutinase are bacterial polyester hydrolases capable of efficiently degrading polyethylene terephthalate (PET) films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate mono-(2-hydroxyethyl) terephthalate (MHET), a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 was employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis showed an increased amount of soluble products with a lower proportion of MHET in the presence of the immobilized TfCa. The results indicated a continuous hydrolysis of the inhibitory MHET by the immobilized TfCa and demonstrated its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films. The dual enzyme system with LC-cutinase produced a 2.4-fold higher amount of degradation products compared to TfCut2 after a reaction time of 24 h confirming the superior activity of his polyester hydrolase against PET films.
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Barth
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Annett
authors.initials: A
authors.lastname: Honak
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Oeser
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Ren
authors.initials: R
authors.lastname: Wei
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Matheus R
authors.initials: MR
authors.lastname: Belisário-Ferrari
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Then
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Schmidt
authors.affiliation: Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Leipzig, Germany. wolfgang.zimmermann@uni-leipzig.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zimmermann
mesh_terms: D055162:Biocatalysis; D043182:Carboxylesterase / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D011093:Polyethylene Terephthalates; D006867:Hydrolases; D043182:Carboxylesterase
keywords: Biocatalysis; Biodegradation; Bioprocess engineering; Polyester hydrolases; Synthetic polymers