title: Frequency of BKC-1-Producing Klebsiella Species Isolates.
abstract: BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. The principal objective of this study was to evaluate the frequency of blaBKC-1 by testing a collection of Klebsiella isolates. Only 2 of 635 Klebsiella isolates (0.3%) carried blaBKC-1 The two BKC-1-producing isolates belonged to clonal complex 442 and possessed identical pulsed-field gel electrophoresis patterns. The blaBKC-1 gene was inserted into a 10-kb plasmid that was identical to the previously reported plasmid, p60136. The BKC-producing K. pneumoniae isolates presented also possessed other mechanisms for beta-lactam resistance, such as genes encoding extended-spectrum beta-lactamases and mutations in the genes ompK35 and ompK36, encoding the major porins.
authors.affiliation: Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, Brazil willamesbrasileiro@hotmail.com.
authors.forename: Willames M B S
authors.initials: WM
authors.lastname: Martins
authors.affiliation: Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Adriana G
authors.initials: AG
authors.lastname: Nicoletti
authors.affiliation: School of Pharmacy, University of São Paulo, São Paulo, Brazil.
authors.forename: Silvia R
authors.initials: SR
authors.lastname: Santos
authors.affiliation: School of Pharmacy, University of São Paulo, São Paulo, Brazil Fleury Diagnostic Medicine, Microbiology Section, São Paulo, Brazil.
authors.forename: Jorge L M
authors.initials: JL
authors.lastname: Sampaio
authors.affiliation: Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Gales
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016521:Electrophoresis, Gel, Pulsed-Field; D007709:Klebsiella / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase