title: Valorization of bakery waste for biocolorant and enzyme production by Monascus purpureus.
abstract: A concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by Monasucus purpureus has been developed. The proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by Hong Kong or other countries. Bakery waste collected from bakery store was used in submerged and solid-state fermentation of Monascus purpureus to produce bio-colorant, which could potentially be applied in food and textile industries. The feasibility of utilizing bakery waste hydrolysate deriving from hydrolytic reactions by Aspergillus awamori and Aspergillus oryzae for fermentative pigment production was investigated. Preliminary data from bakery waste hydrolysate experiment presented that the highest pigment yield (about 24AU/g glucose) was obtained with bakery waste hydrolysate containing 5g/L initial glucose. Results from the solid state fermentation studies presented that the highest activity of glucoamylase and protease achieved was 8U/g and 117U/g respectively, at an initial moisture content of 55% and 65% respectively at 30°C incubation temperature. The outcome from this study demonstrated that Monascus purpureus constitutes a promising host for bio-colorant and enzyme production using recovered sugars and amino acids from bakery waste.
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, Hong Kong.
authors.forename: Md Ariful
authors.initials: MA
authors.lastname: Haque
authors.affiliation: Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Kachrimanidou
authors.affiliation: Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece.
authors.forename: Apostolis
authors.initials: A
authors.lastname: Koutinas
authors.affiliation: School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, Hong Kong. Electronic address: carollin@cityu.edu.hk.
authors.forename: Carol Sze Ki
authors.initials: CSK
authors.lastname: Lin
mesh_terms: D001709:Biotechnology; D003296:Cooking; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose; D006868:Hydrolysis; D007220:Industrial Waste; D032741:Monascus / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D010447:Peptide Hydrolases; D010860:Pigments, Biological / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D007220:Industrial Waste; D010860:Pigments, Biological; D005087:Glucan 1,4-alpha-Glucosidase; D010447:Peptide Hydrolases; D005947:Glucose
keywords: Bakery waste; Glucoamylase; Hydrolysate; Protease