title: Evaluation and quantitative analysis of different growth periods of herb-arbor intercropping systems using HPLC and UV-vis methods coupled with chemometrics.
abstract: As a result of the pressure from population explosion, agricultural land resources require further protecting and rationally utilizing. Intercropping technique has been widely applied for agricultural production to save cultivated area, improve crop quality, and promote agriculture economy. In this study, we employed high-performance liquid chromatography (HPLC) and ultraviolet-visible spectroscopy (UV-vis) combined with chemometrics for determination and qualitative evaluation of several kinds of intercropping system with Gentiana rigescens Franch. ex Hemsl. (GR), which is used as an hepatic protector in local communities in China. Results revealed that GR in a Camellia sinensis intercropping system contained most gentiopicroside, sweroside, and total active constituents (six chemical indicators), whose content reached 91.09 ± 3.54, 1.03 ± 0.06, and 104.05 ± 6.48 mg g(-1), respectively. The two applied quantitative and qualitative methods reciprocally verified that GR with 2 years of growth period performed better in terms of quality than 1 year, collectively.
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.|College of Traditional Chinese Medicine, Yunnan College of Traditional Chinese Medicine, Kunming, 650500, China.
authors.forename: Bo-Wen
authors.initials: BW
authors.lastname: Chu
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.
authors.forename: Zhi-Tian
authors.initials: ZT
authors.lastname: Zuo
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China. boletus@126.com.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China. boletus@126.com.
authors.forename: Yuan-Zhong
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China. wanyili2012@126.com.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China. wanyili2012@126.com.|College of Traditional Chinese Medicine, Yunnan College of Traditional Chinese Medicine, Kunming, 650500, China. wanyili2012@126.com.
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Li
mesh_terms: D000383:Agriculture / Q000379:methods*; D028241:Camellia sinensis; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030012:Gentiana / Q000737:chemistry*; D057888:Iridoid Glucosides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal; D013662:Tea
chemical_list: D057888:Iridoid Glucosides; D010936:Plant Extracts; D013662:Tea; C012997:gentiopicroside; C049412:sweroside
keywords: Chemometrics; Gentiana rigescens; Intercropping; Quantitative analysis