title: Diversity and distribution of catechol 2, 3-dioxygenase genes in surface sediments of the Bohai Sea.
abstract: Catechol 2, 3-dioxygenase (C23O) is the key enzyme for aerobic aromatic degradation. Based on clone libraries and quantitative real-time polymerase chain reaction, we characterized diversity and distribution patterns of C23O genes in surface sediments of the Bohai Sea. The results showed that sediments of the Bohai Sea were dominated by genes related to C23O subfamily I.2.A. The samples from wastewater discharge area (DG) and aquaculture farm (KL) showed distinct composition of C23O genes when compared to the samples from Bohai Bay (BH), and total organic carbon was a crucial determinant accounted for the composition variation. C6BH12-38 and C2BH2-35 displayed the highest gene copies and highest ratios to the 16S rRNA genes in KL, and they might prefer biologically labile aromatic hydrocarbons via aquaculture inputs. Meanwhile, C7BH3-48 showed the highest gene copies and highest ratios to the 16S rRNA genes in DG, and this could be selective effect of organic loadings from wastewater discharge. An evident increase in C6BH12-38 and C7BH3-48 gene copies and reduction in diversity of C23O genes in DG and KL indicated composition perturbations of C23O genes and potential loss in functional redundancy. We suggest that ecological habitat and trophic specificity could shape the distribution of C23O genes in the Bohai Sea sediments.
authors.affiliation: The Key Laboratory of Marine Bioactive Substances, The First Institute of Oceanography, State Oceanic Administration, Marine Ecology Research Center, Qingdao 266061, China.
authors.forename: Peiqing
authors.initials: P
authors.lastname: He
authors.affiliation: Key laboratory of State Oceanic Administration for Marine Sedimentology & Environmental Geology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Key laboratory of State Oceanic Administration for Marine Sedimentology & Environmental Geology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China jihliu@fio.org.cn.
authors.forename: Jihua
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key laboratory of State Oceanic Administration for Marine Sedimentology & Environmental Geology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
authors.forename: Yazhi
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Key laboratory of State Oceanic Administration for Marine Sedimentology & Environmental Geology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
authors.forename: Xisheng
authors.initials: X
authors.lastname: Fang
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics*; D002396:Catechols / Q000378:metabolism; D002681:China; D015723:Gene Library; D014644:Genetic Variation; D019015:Geologic Sediments / Q000382:microbiology*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D009792:Oceans and Seas; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D050557:Catechol 2,3-Dioxygenase; D002396:Catechols; D006841:Hydrocarbons, Aromatic; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Catechol 2, 3-dioxygenase; aromatic hydrocarbons; diversity; the Bohai Sea