title: Seminal plasma HSPA2 mRNA content is associated with semen quality.
abstract: OBJECTIVE
  Cell-free mRNAs (cfmRNAs) were quantitatively measured in human seminal plasma and its relationship with semen quality was investigated.
  
  
  METHODS
  Herein, a prospectively, controlled investigation was performed to study seminal plasma HSPA2 and uPA cfmRNA alterations between 21 asthenozoospermic patients and 16 normozoospermic individuals. Standard semen analysis was performed and seminal plasma cfmRNAs content was measured by real-time quantitative PCR. In addition, the regression analysis between seminal plasma cfmRNAs expression and semen parameters was performed.
  
  
  RESULTS
  Seminal plasma HSPA2, but not uPA cfmRNA indicated significant difference between normozoospermia and asthenozoospermia men (P = 0.02444 and 0.07811, respectively). Negative correlation between HSPA2 cfmRNA and sperm motility (R (2) = 0.213, P = 0.004) as well as sperm concentration (R (2) = 0.133, P = 0.026) were revealed. However, no correlation was found between seminal plasma uPA cfmRNA content and semen parameters.
  
  
  CONCLUSIONS
  Our data suggest that seminal plasma HSPA2 cfmRNA is different between asthenozoospermic and normozoospermic individuals and it might be an indicator for semen quality.
authors.affiliation: Reproductive Medicine Center, Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China, 310006.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Reproductive Medicine Center, Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China, 310006.
authors.forename: Lejun
authors.initials: L
authors.lastname: Li
authors.affiliation: Reproductive Medicine Center, Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China, 310006.
authors.forename: Fengbin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Reproductive Medicine Center, Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China, 310006. xuj@zju.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012661:Semen / Q000166:cytology; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics
chemical_list: D018840:HSP70 Heat-Shock Proteins; C091879:HSPA2 protein, human; D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator
keywords: Asthenozoospermia; Heat shock protein A2; Normozoospermia; Seminal plasma cell-free mRNA; Urokinase-type plasminogen activator