title: Cascade enzymatic catalysis in poly(acrylic acid) brushes-nanospherical silica for glucose detection.
abstract: The ultrasensitive monitoring of glucose with a fast and accurate method is significant in potential therapeutics and optimizes protein biosynthesis. Incorporation of enzyme into matrix is considered as promising candidates for constructing highly sensitive glucose-responsive systems. In this study, three-dimensional poly(acrylic acid) brushes-nanospherical silica (PAA-nano silica) with high amplification capability and stability were used to covalently immobilize bienzymes for cascade enzymatic catalysis. The major advantages of PAA-nano silica-bienzyme co-incorporation is that the enzymes are proximity distribution, and such close confinement both minimized the diffusion of intermediates among the enzymes in the consecutive reaction and improve the utilization efficiency of enzymes, thereby enhancing the overall reaction efficiency and specificity. Thus, this present bienzymatic biosensor shows robust signal amplification and ultrasensitivity of glucose-responsive properties with a detection limit of 0.04μM.
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Rongmei
authors.initials: R
authors.lastname: Kong
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xia
authors.affiliation: The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, PR China. Electronic address: fengliquhn@hotmail.com.
authors.forename: Fengli
authors.initials: F
authors.lastname: Qu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D055162:Biocatalysis; D015374:Biosensing Techniques; D004058:Diffusion; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; C006903:carbopol 940; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose
keywords: Bienzyme catalysis; Glucose detection; Glucose oxidase; Horseradish peroxidase; Poly(acrylic acid) brushes-nanospherical silica