title: Modified Dietary Fiber from Cassava Pulp and Assessment of Mercury Bioaccessibility and Intestinal Uptake Using an In Vitro Digestion/Caco-2 Model System.
abstract: The ability of modified dietary fiber (MDF) generated from cassava pulp to modulate the bioaccessibility and intestinal absorption of heavy metals may be helpful to mitigate health risk associated with select foods including select fish high in methyl mercury. Using a coupled in vitro digestion/Caco-2 human intestinal cell model, the reduction of fish mercury bioaccessibility and intestinal uptake by MDF was investiaged. MDF was prepared from cassava pulp, a byproduct of tapioca production. The highest yield (79.68%) of MDF was obtained by enzymatic digestion with 0.1% α-amylase (w/v), 0.1% amyloglucosidase (v/v) and 1% neutrase (v/v). MDF and fish tissue were subjected to in vitro digestion and results suggest that MDF may reduce mercury bioaccessibility from fish to 34% to 85% compared to control in a dose-dependent manner. Additionally, accumulation of mercury from digesta containing fish and MDF was only modestly impacted by the presence of MDF. In conclusion, MDF prepared from cassava pulp may be useful as an ingredient to reduce mercury bioavailability from food such as fish specifically by inhibiting mercury transfer to the bioaccessibile fraction during digestion.
authors.affiliation: School of Food Technology, Inst. of Agricultural Technology, Suranare Univ. of Technology, Nakhon Ratchasima, Thailand.
authors.forename: Natta
authors.initials: N
authors.lastname: Kachenpukdee
authors.affiliation: Dept. of Nutrition Science, Purdue Univ, West Lafayette, Ind., U.S.A.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Santerre
authors.affiliation: Dept. of Food Science, Purdue Univ, West Lafayette, Ind., U.S.A.
authors.forename: Mario G
authors.initials: MG
authors.lastname: Ferruzzi
authors.affiliation: School of Food Technology, Inst. of Agricultural Technology, Suranare Univ. of Technology, Nakhon Ratchasima, Thailand.
authors.forename: Ratchadaporn
authors.initials: R
authors.lastname: Oonsivilai
mesh_terms: D000818:Animals; D001682:Biological Availability; D018938:Caco-2 Cells; D003106:Colon / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism / Q000494:pharmacology*; D004063:Digestion; D005399:Fishes; D005506:Food Contamination; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D002365:Manihot / Q000737:chemistry*; D008628:Mercury / Q000378:metabolism* / Q000493:pharmacokinetics; D008666:Metalloendopeptidases / Q000378:metabolism; D008767:Methylmercury Compounds / Q000378:metabolism / Q000493:pharmacokinetics; D035281:Plant Tubers / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D004043:Dietary Fiber; D008767:Methylmercury Compounds; C073972:dimethyl mercury; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase; D008666:Metalloendopeptidases; C044194:microbial metalloproteinases; D008628:Mercury
keywords: Caco-2 cell line; bioaccessibility; bioavailability; cassava pulp; dietary fiber; in vitro digestion; modified dietary fiber