title: Optimization of the extraction of polysaccharides from tobacco waste and their biological activities.
abstract: A response surface methodology was used to optimize the parameters for extracting the polysaccharides from tobacco waste (TWPs) using hot water. The extraction process, carried out under the following optimized parameters: an extraction temperature of 90°C, a ratio of water to raw material of 54, and an extraction time of 115min, allowed an experimental yield of 28.32±1.78%. The chemical composition analysis showed that TWPs were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose with the following molecular ratio: 1.00:2.69:1.29:2.29:5.23:6.90:3.92. The molecular weights of its four major fractions were 0.558, 1.015, 16.286, and 151.194kDa. Bioactivity experiments showed that TWPs not only decreased the reactive oxygen species level in salt-stressed tomato seedlings, but also possessed significant antioxidant activities in vitro. Antioxidant activity in vivo further showed that TWPs could significantly increase the activities of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and decrease the level of malondialodehyde (MDA). In addition, according to the acute toxicity test, TWPs did not cause behavioral changes or any death of mice. This study provides an effective method to utilize tobacco waste resources.
authors.affiliation: Henan Agricultural University, National Tobacco Cultivation and Physiology and Biochemistry Research Centre, Key Laboratory for Tobacco Cultivation of Tobacco Industry, Zhengzhou 450002, China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Tobacco Industry Co., Ltd., Technology Research and Development Center in Gansu province, Lanzhou 730050, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Guangxi Zhuang Autonomous Region Provincial Branch of CNTC, Nanning 530022, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Wang
authors.affiliation: Nanyang Branch of Henan National Tobacco Corporation, Nanyang 473000, China.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Technology Center of Chinese Tobacco Industrial Company in Henan, Zhengzhou 450000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.affiliation: Henan Provincial Branch of CNTC, Zhengzhou 450052, China. Electronic address: macong9666@163.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Technology Center of Chinese Tobacco Industrial Company in Henan, Zhengzhou 450000, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000254:growth & development; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D036226:Seedlings / Q000254:growth & development; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Polysaccharide; Response surface methodology; Tobacco waste