title: Implementation of Salmonella serotype determination using pulsed-field gel electrophoresis in a state public health laboratory.
abstract: We examined the use of pulsed-field gel electrophoresis (PFGE) to predict serotype for Salmonella isolates. Between 2012 and 2014 we assessed 4481 isolates, resulting in >90% assigned serotypes. PFGE is efficient for determining serotype in the majority of cases and results in expedited serotype determination, as well as cost savings.
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Dianna J
authors.initials: DJ
authors.lastname: Bopp
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Baker
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Lisa
authors.initials: L
authors.lastname: Thompson
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Amy
authors.initials: A
authors.lastname: Saylors
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Root
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Leeanna
authors.initials: L
authors.lastname: Armstrong
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Kara
authors.initials: K
authors.lastname: Mitchell
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208.
authors.forename: Nellie B
authors.initials: NB
authors.lastname: Dumas
authors.affiliation: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave. Albany, NY 12208. Electronic address: kimberlee.musser@health.ny.gov.
authors.forename: Kimberlee Arruda
authors.initials: KA
authors.lastname: Musser
mesh_terms: D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000191:economics / Q000379:methods*; D006801:Humans; D012475:Salmonella / Q000145:classification* / Q000235:genetics*; D012703:Serotyping / Q000379:methods*
keywords: Cost savings; PFGE; Salmonella; Serotyping