title: Evaluation and validation of the use of a molecularly imprinted polymer coupled to LC-MS for benzylpenicillin determination in meat samples.
abstract: This article describes a full analytical method for the clean-up and detection of benzylpenicillin in chicken and beef meat samples using a previously developed molecularly imprinted polymer in a solid-phase extraction cleanup step followed by analysis using LC-MS/MS. The method was validated based on the criteria and the requirements of the European Commission Decision 2002/657/EC. This validation revealed method performance characteristics that meet all the criteria in the Decision with limits of detection for chicken and beef meat samples of 6.2 and 14.4μg/kg, respectively, 8 and 3.5 times lower than the MRL. This method holds strong potential when a specific cleanup of benzylpenicillin is required, because with slight modifications it is also applicable in a milk matrix [1].
authors.affiliation: Institute for Agricultural and Fisheries Research (ILVO) Technology and Food Science Unit, Brusselsesteenweg 370, Melle B-9090, Belgium. Electronic address: geert.vanroyen@ilvo.vlaanderen.be.
authors.forename: Geert
authors.initials: G
authors.lastname: Van Royen
authors.affiliation: Ghent University, Polymer Chemistry and Biomaterials Group, Department of Organic and Macromolecular Chemistry, Polymer Chemistry and Biomaterials Research Group (PBM), Krijgslaan 281 S4, Gent B-9000, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.affiliation: Institute for Agricultural and Fisheries Research (ILVO) Technology and Food Science Unit, Brusselsesteenweg 370, Melle B-9090, Belgium.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Van Weyenberg
authors.affiliation: Institute for Agricultural and Fisheries Research (ILVO) Technology and Food Science Unit, Brusselsesteenweg 370, Melle B-9090, Belgium.
authors.forename: Els
authors.initials: E
authors.lastname: Daeseleire
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat; D054802:Molecular Imprinting / Q000379:methods*; D010400:Penicillin G / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013552:Swine
chemical_list: D010400:Penicillin G
keywords: Benzylpenicillin; MISPE; Meat; Molecularly imprinted polymer; Validation