title: Coevolution analysis of Hepatitis C virus genome to identify the structural and functional dependency network of viral proteins.
abstract: A novel computational approach of coevolution analysis allowed us to reconstruct the protein-protein interaction network of the Hepatitis C Virus (HCV) at the residue resolution. For the first time, coevolution analysis of an entire viral genome was realized, based on a limited set of protein sequences with high sequence identity within genotypes. The identified coevolving residues constitute highly relevant predictions of protein-protein interactions for further experimental identification of HCV protein complexes. The method can be used to analyse other viral genomes and to predict the associated protein interaction networks.
authors.affiliation: Sorbonne Universités, UPMC-Univ P6, CNRS, Laboratoire de Biologie Computationnelle et Quantitative - UMR 7238, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Champeimont
authors.affiliation: Sorbonne Universités, UPMC-Univ P6, CNRS, Laboratoire de Biologie Computationnelle et Quantitative - UMR 7238, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Laine
authors.affiliation: Sorbonne Universités, UPMC-Univ P6, CNRS, Laboratoire de Biologie Computationnelle et Quantitative - UMR 7238, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
authors.forename: Shuang-Wei
authors.initials: SW
authors.lastname: Hu
authors.affiliation: CNRS, UMR5086, Bases Moléculaires et Structurales des Systèmes Infectieux, Institut de Biologie et Chimie des Protéines, 7 Passage du Vercors, Cedex 07, F-69367 Lyon, France.|LABEX Ecofect, Université de Lyon, Lyon, France.
authors.forename: Francois
authors.initials: F
authors.lastname: Penin
authors.affiliation: Sorbonne Universités, UPMC-Univ P6, CNRS, Laboratoire de Biologie Computationnelle et Quantitative - UMR 7238, 15 rue de l'Ecole de Médecine, 75006 Paris, France.|Institut Universitaire de France, 75005, Paris, France.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Carbone
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D019295:Computational Biology / Q000379:methods*; D019143:Evolution, Molecular; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D060066:Protein Interaction Maps; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins