title: Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross-amplification in four other species.
abstract: OBJECTIVE
  Twenty-six microsatellite (simple sequence repeat [SSR]) markers were characterized in Psychotria homalosperma (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species.
  
  
  RESULTS
  Using next-generation sequencing, we developed 26 SSR markers for P. homalosperma with perfect motifs from di- to pentanucleotide repeats. Of these, the Chichijima and Hahajima island populations of P. homalosperma had mean allele numbers of 6.50 and 6.81, respectively. The mean expected heterozygosities were 0.578 and 0.606, respectively. In addition, 10 and eight of these markers were successfully amplified for P. boninensis and P. serpens, respectively, occurring in the same or adjacent areas.
  
  
  CONCLUSIONS
  The SSR markers developed in this study will be useful for future studies concerning the population genetics of P. homalosperma and will facilitate the development of a conservation strategy.
authors.affiliation: Department of Wildlife Biology, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sugai
authors.affiliation: Okinawa College, National Institute of Technology, 905 Henoko, Nago, Okinawa 905-2192, Japan; Department of Botany, University of Hawaii, 3190 Maile Way, Honolulu, Hawaii 96822 USA.
authors.forename: Kenta
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Makino Herbarium, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kato
authors.affiliation: Makino Herbarium, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugawara
keywords: Psychotria homalosperma; Rubiaceae; de novo sequencing; gene flow; heterostyly; microsatellites