title: Human soluble delta-like 1 homolog exerts antitumor effects in vitro and in vivo.
abstract: Proteolysis of delta-like 1 homolog (DLK1), a cell-surface transmembrane protein, produces an active soluble form of DLK1 (sDLK1). Both membrane-bound DLK1 and sDLK1 modulate multiple developmental processes including adipogenesis, osteogenesis, chondrogenesis and myogenesis. However, cancer-related functions of DLK1 have not yet been established. We thus evaluated the roles of extracellular sDLK1, comprising six EGF-like domains and juxtamembrane regions, in human pancreatic cancer MIA PaCa-2 cells in vitro and in vivo. We observed that sDLK1 exerted antitumor effects not only in cancer cell migration and anchorage-independent cell growth but also in in vivo tumor growth.
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, 34141, Republic of Korea.
authors.forename: Donghee
authors.initials: D
authors.lastname: Lee
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, 34141, Republic of Korea.
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Yoon
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, 34141, Republic of Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, 34141, Republic of Korea.
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Jo
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, 34141, Republic of Korea.
authors.forename: Bum-Chan
authors.initials: BC
authors.lastname: Park
authors.affiliation: Department of Biological Sciences and Biotechnology, Hannam University, Daejeon, 34054, Republic of Korea.
authors.forename: In Seop
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences and Biotechnology, Hannam University, Daejeon, 34054, Republic of Korea.
authors.forename: Yunseon
authors.initials: Y
authors.lastname: Choi
authors.affiliation: ANRT, Inc., PAI CHAI University Industry-Academic Cooperation Foundation Building, Daejeon, 34015, Republic of Korea. Electronic address: limjc89@gmail.com.
authors.forename: Jung Chae
authors.initials: JC
authors.lastname: Lim
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, 34141, Republic of Korea. Electronic address: ywpark@kribb.re.kr.
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Park
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D002135:Calcium-Binding Proteins; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D010179:Pancreas / Q000378:metabolism* / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D000072417:Protein Domains; D059748:Proteolysis
chemical_list: D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins
keywords: Cancer; Delta-like 1 homolog; Extracellular soluble DLK1; Membrane protein; Proteolysis