title: HnRNP A1 is Involved in Deep Vein Thrombosis Patients with Behçet's Disease.
abstract: OBJECTIVE
  The aim of this study was to verify the hypothesis originated from bioinformatics and literature reviews that hnNRP A1 may be a new immune target of Behçet's disease (BD).
  
  
  METHODS
  First, bioinformatics was used to show the correlation between hnRNP A1 and A2/B1 in amino acid sequences and three dimensional structures. Second, hnRNP A1 was expressed, purified, and immunologically confirmed by systematic immunology methods including: Western blotting, immunoprecipitation and Dot-ELISA. Then, ELISA was used to screen the anti-hnRNP A1 autoantibodies in newly confirmed clinical samples and the clinical significance was compared between anti-hnRNP A1 antibody positive and negative groups. Finally, the endothelial cells antigen profile of one anti-hnRNP A1 antibody positive BD patient was detected using immunoprecipitation with liquid chromatography tandem mass spectrometry (LC-TMS).
  
  
  RESULTS
  In total 720 subjects enrolled and tested in this study. Our results demonstrated hnRNP A1 as a new immune target of BD. The reactivity of BD serum IgG antibodies against hnRNP A1 was significantly higher than healthy controls (P<0.0001), and deep vein thrombosis (DVT) showed a significant higher in the anti-hnRNP A1 antibodies positive group (P<0.05).
authors.affiliation: 112 Lab, School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: 112 Lab, School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Rheumatology Department of Shanxi DaYi Hospital, Shanxi Academy of Medical Sciences, Taiyuan 030032, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: 112 Lab, School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Chunhe
authors.initials: C
authors.lastname: Yang
authors.affiliation: ImmunoHunt Corporation, Beijing 100070, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Chen
authors.affiliation: Rheumatology Department of Shanxi DaYi Hospital, Shanxi Academy of Medical Sciences, Taiyuan 030032, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ma
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing 100853, China. Electronic address: tianyp61@gmail.com.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Tian
authors.affiliation: 112 Lab, School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China. Electronic address: hwdu93@126.com.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000276:immunology; D001528:Behcet Syndrome / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D005260:Female; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D020246:Venous Thrombosis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B
keywords: Autoantigen; Behçet's disease; Bioinformatics; HnRNP A1