title: A multi-laboratory comparison of blood dendritic cell populations.
abstract: HLDA10 collated a panel of monoclonal antibodies (mAbs) that primarily recognised molecules on human myeloid cell and dendritic cell (DC) populations. As part of the studies, we validated a backbone of mAbs to delineate monocyte and DC populations from peripheral blood. The mAb backbone allowed identification of monocyte and DC subsets using fluorochromes that were compatible with most 'off the shelf' or routine flow cytometers. Three laboratories used this mAb backbone to assess the HLDA10 panel on blood monocytes and DCs. Each laboratory was provided with enough mAbs to perform five repeat experiments. The data were collated and analysed using Spanning-tree Progression Analysis of Density-normalised Events (SPADE). The data were interrogated for inter- and intra-laboratory variability. The results highlight the definition of DC populations using current readily available reagents. This collaborative process provides the broader scientific community with an invaluable data set that validates mAbs to leucocyte surface molecules.
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Phillip Dieter
authors.initials: PD
authors.lastname: Fromm
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute , Sydney, NSW, Australia.
authors.forename: Fiona
authors.initials: F
authors.lastname: Kupresanin
authors.affiliation: School of Biological Sciences, The University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Auckland, New Zealand.
authors.forename: Anna Elizabeth Stella
authors.initials: AE
authors.lastname: Brooks
authors.affiliation: School of Biological Sciences, The University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Auckland, New Zealand.
authors.forename: Peter Rodney
authors.initials: PR
authors.lastname: Dunbar
authors.affiliation: Human DC Lab, Institute of Cellular Medicine, Newcastle University , Newcastle upon Tyne, UK.
authors.forename: Muzifilla
authors.initials: M
authors.lastname: Haniffa
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Derek Nigel John
authors.initials: DN
authors.lastname: Hart
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Georgina Jane
authors.initials: GJ
authors.lastname: Clark