title: Drosophila Ana1 is required for centrosome assembly and centriole elongation.
abstract: Centrioles organise centrosomes and cilia, and these organelles have an important role in many cell processes. In flies, the centriole protein Ana1 is required for the assembly of functional centrosomes and cilia. It has recently been shown that Cep135 (also known as Bld10) initially recruits Ana1 to newly formed centrioles, and that Ana1 then recruits Asl (known as Cep152 in mammals) to promote the conversion of these centrioles into centrosomes. Here, we show that ana1 mutants lack detectable centrosomes in vivo, that Ana1 is irreversibly incorporated into centrioles during their assembly and appears to play a more important role in maintaining Asl at centrioles than in initially recruiting Asl to centrioles. Unexpectedly, we also find that Ana1 promotes centriole elongation in a dose-dependent manner: centrioles are shorter when Ana1 dosage is reduced and are longer when Ana1 is overexpressed. This latter function of Ana1 appears to be distinct from its role in centrosome and cilium function, as a GFP-Ana1 fusion lacking the N-terminal 639 amino acids of the protein can support centrosome assembly and cilium function but cannot promote centriole over-elongation when overexpressed.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Saroj
authors.initials: S
authors.lastname: Saurya
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Hélio
authors.initials: H
authors.lastname: Roque
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Zsofia A
authors.initials: ZA
authors.lastname: Novak
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Wainman
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Mustafa G
authors.initials: MG
authors.lastname: Aydogan
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Adam
authors.initials: A
authors.lastname: Volanakis
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Boris
authors.initials: B
authors.lastname: Sieber
authors.affiliation: Micron Oxford Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: David Miguel Susano
authors.initials: DM
authors.lastname: Pinto
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK jordan.raff@path.ox.ac.uk.
authors.forename: Jordan W
authors.identifier: 0000-0002-4689-1297
authors.initials: JW
authors.lastname: Raff
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D002502:Centrioles / Q000235:genetics*; D018385:Centrosome / Q000378:metabolism; D002923:Cilia / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008938:Mitosis / Q000235:genetics; D050505:Mutant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: C539309:Asl protein, Drosophila; C542499:CEP135 protein, Drosophila; D029721:Drosophila Proteins; D006023:Glycoproteins; D050505:Mutant Proteins; C082255:ana protein, Drosophila
keywords: Ana1; Centriole; Centriole elongation; Centriole structure; Centrosome; Cep295