title: Syndecan-4, a PRRSV attachment factor, mediates PRRSV entry through its interaction with EGFR.
abstract: The causative agent of porcine reproductive and respiratory syndrome is the PRRS virus (PRRSV), an enveloped, single-stranded and positive-sense RNA virus. The host factors and mechanisms that are involved in PRRSV entry are still largely unknown. In our present studies, we found that syndecan-4, one of the heparan sulfate proteoglycans, plays a critical role in PRRSV entry, especially in PRRSV attachment. Moreover, EGFR interacts with syndecan-4 in MACR-145 cells and disruption of their interaction impaired PRRSV entry. Furthermore, EGFR inhibitor AG1478 or syndecan-4 derived peptide SSTN87-131 inhibited syndecan-4 endocytosis induced by PRRSV entry. Altogether, syndecan-4, a PRRSV attachment factor, mediated PRRSV entry by interacting with EGFR.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, China. Electronic address: xybwr@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ni
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, China.
authors.forename: Chang-Chao
authors.initials: CC
authors.lastname: Huan
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, China.
authors.forename: Jia-Qiang
authors.initials: JQ
authors.lastname: Wu
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Science, China.
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Wen
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China.
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Tong
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, China.
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China. Electronic address: xmao@njau.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mao
mesh_terms: D000818:Animals; D002460:Cell Line; D004705:Endocytosis; D066246:ErbB Receptors / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000503:physiopathology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D060066:Protein Interaction Maps; D013552:Swine / Q000378:metabolism / Q000821:virology*; D053671:Syndecan-4 / Q000378:metabolism*; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D053671:Syndecan-4; D066246:ErbB Receptors
keywords: Attachment; EGFR; Entry; Porcine reproductive and respiratory syndrome virus; Syndecan-4