title: A novel method for determining the relation between nasal polyposis and oxidative stress: the thiol/disulphide homeostasis.
abstract: CONCLUSIONS
  It is thought that oxidative stress may be the major cause of the increase in the oxide thiol form in the study group. The relationship between oxidative stress status and dynamic thiol/disulphide in nasal polyposis now needs to be investigated.
  
  
  OBJECTIVE
  The purpose of this study was to evaluate the relation between nasal polyposis and thiol/disulphide homeostasis, used as a marker of oxidative stress, by measuring that exchange using a novel technique.
  
  
  METHODS
  The study group consisted of 40 patients (mean age = 46.75 ± 13.92 years) with bilateral nasal polyposis patients admitted to the hospital. The control group consisted of 31 (mean age = 43.20 ± 5.68 years) age, sex, and body mass index matched healthy subjects. Thiol/disulphide homeostasis concentrations were measured using a newly-developed method (Erel & Neselioglu).
  
  
  RESULTS
  Native thiol and total thiol levels were lower in the study group compared to the control group (native thiol = 415.8 ± 69.1 μmol/L vs 448.7 ± 37.5 μmol/L, p < 0.05; total thiol = 449.02 ± 72.0 μmol/L vs 477.28 ± 44.5 μmol/L, p < 0.05, respectively). Disulphide level and the disulphide/native thiol and disulphide/total thiol ratios were higher in the study group compared to the control group (disulphide = 16.58 ± 5.04 μmol/L vs 14.28 ± 5.3 μmol/L, p < 0.05; disulphide/native thiol ratio = 4.07 ± 1.52% vs 3.14 ± 1.04%, p < 0.05, disulphide/total thiol ratio = 3.73 ± 1.23% vs 2.94 ± 0.92%, p < 0.05, respectively).
authors.affiliation: a Department of Otorhinolaryngology , Erzurum Regional Training and Research Hospital , Erzurum , Turkey.
authors.forename: Eda
authors.initials: E
authors.lastname: Şimşek
authors.affiliation: b Faculty of Medicine, Department of Clinical Biochemistry , Yildirim Beyazit University , Ankara , Turkey.
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
authors.affiliation: b Faculty of Medicine, Department of Clinical Biochemistry , Yildirim Beyazit University , Ankara , Turkey.
authors.forename: Cemile Koca
authors.initials: CK
authors.lastname: Bicer
authors.affiliation: c Department of Endocrinology , Erzurum Regional Training and Research Hospital , Erzurum , Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Çarlıoğlu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004220:Disulfides / Q000097:blood*; D005260:Female; D006706:Homeostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000097:blood*; D018384:Oxidative Stress; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: Disulphide; nasal polyposis; oxidative stress; thiol