title: Polyaminoacid nanocapsules for drug delivery to the lymphatic system: Effect of the particle size.
abstract: Previous work by our group showed the possibility to reduce the toxicity of docetaxel upon its encapsulation in polyaminoacid nanocapsules with a size of 200nm. The objective of this study was to elucidate whether a reduction in the nanocapsules size might facilitate their access to the lymphatic system. To do so, we analyzed the effect of several formulation parameters on the characteristics of polyglutamic acid, PEGylated polyglutamic acid and polyasparagine nanocapsules. From these experiments, we could identify the best conditions to produce nanocapsules with a small size (close to 100nm) and adequate capacity to encapsulate and sustain the release of the antitumor drug docetaxel. Moreover, the results of the stability study made evident the critical role of the polyaminoacid shell on the colloidal stability of the nanocapsules in biologically relevant media. Finally, we studied the influence of the particle size (100nm vs. 200nm) on the biodistribution of PGA-PEG nanocapsules following subcutaneous injection. The results showed that the 100 nm-size nanocapsules accumulate faster in the lymph nodes, than those with a size of 200nm. In summary, these data suggest the potential of 100nm-size polyaminoacid nanocapsules as lymphatic drug delivery carriers.
authors.affiliation: Nanobiofar Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Abellan-Pose
authors.affiliation: Nanobiofar Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Teijeiro-Valiño
authors.affiliation: Cellular Neurobiology and Molecular Chemistry of the Central Nervous System Group, University of Castilla-La Mancha, 02071 Campus of Albacete, Albacete, Spain.
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Santander-Ortega
authors.affiliation: Cell Cycle and Oncology Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Erea
authors.initials: E
authors.lastname: Borrajo
authors.affiliation: Cell Cycle and Oncology Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Anxo
authors.initials: A
authors.lastname: Vidal
authors.affiliation: Nanobiofar Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: Garcia-Fuentes
authors.affiliation: Nanobiofar Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain. Electronic address: noemi.csaba@usc.es.
authors.forename: Noémi
authors.initials: N
authors.lastname: Csaba
authors.affiliation: Nanobiofar Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15706 Campus Vida, Santiago de Compostela, Spain. Electronic address: mariaj.alonso@usc.es.
authors.forename: María José
authors.initials: MJ
authors.lastname: Alonso
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D002626:Chemistry, Pharmaceutical / Q000379:methods; D000077143:Docetaxel; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D008208:Lymphatic System / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry*; D043823:Taxoids / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D053769:Nanocapsules; D010455:Peptides; D043823:Taxoids; D000077143:Docetaxel; D011099:Polyglutamic Acid; C045164:polyasparagine; D011092:Polyethylene Glycols
keywords: Lymphatic targeting; Particle size; Polyaminoacid nanocapsules