title: Involvement of TLR6 in the induction of COX-2, PGE2 and IL-10 in macrophages by lipids from virulent S2P and attenuated R1A Babesia bovis strains.
abstract: Toll like receptors (TLRs) are involved in the modulation of diverse host genes expression through a complex network of signalling events that allow for an appropriate response to a microbial pathogen. In the present work we used TLR6KO mice in order to study the role of TLR6 in the immune discrimination of lipids from two Babesia bovis strains, attenuated R1A (LA) and virulent S2P (LV), and the consequent macrophage activation. We demonstrated that TLR6 is required for lipid body induction in murine peritoneal macrophages by both LA and LV. Interestingly, as regards IL-10 and COX-2/PGE2 pathway induction by LA and LV, we observed differences in the biological effects produced by these lipid extracts. Our results indicate a role of TLR6 in the down-modulation of these immunoregulators only in the case of LA, whereas this receptor was not implicated in pro-inflammatory TNFα, IL-6 and KC release induced by LA. Remarkably, LV did not exert the down-modulatory effect observed for LA, supporting the notion that LA and LV possess different lipid composition that could correlate with the polar pathogenic effect of both B. bovis strains.
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, UBA, CONICET, Facultad de Medicina, Paraguay 2155 piso 13, C1121ABG Buenos Aires, Argentina. Electronic address: paradife@fmed.uba.ar.
authors.forename: G
authors.initials: G
authors.lastname: Gimenez
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, UBA, CONICET, Facultad de Medicina, Paraguay 2155 piso 13, C1121ABG Buenos Aires, Argentina.
authors.forename: M L
authors.initials: ML
authors.lastname: Belaunzarán
authors.affiliation: Laboratório de Imunologia e Inflamação, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Distrito Federal, Brazil.
authors.forename: K G
authors.initials: KG
authors.lastname: Magalhães
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, UBA, CONICET, Facultad de Medicina, Paraguay 2155 piso 13, C1121ABG Buenos Aires, Argentina.
authors.forename: C V
authors.initials: CV
authors.lastname: Poncini
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, UBA, CONICET, Facultad de Medicina, Paraguay 2155 piso 13, C1121ABG Buenos Aires, Argentina.
authors.forename: E M
authors.initials: EM
authors.lastname: Lammel
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, UBA, CONICET, Facultad de Medicina, Paraguay 2155 piso 13, C1121ABG Buenos Aires, Argentina.
authors.forename: S M
authors.initials: SM
authors.lastname: González Cappa
authors.affiliation: Laboratório de Imunofarmacologia, Instituto Oswaldo Cruz-Fundaçao Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: P T
authors.initials: PT
authors.lastname: Bozza
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica, UBA, CONICET, Facultad de Medicina, Paraguay 2155 piso 13, C1121ABG Buenos Aires, Argentina.
authors.forename: E L D
authors.initials: EL
authors.lastname: Isola
mesh_terms: D000818:Animals; D016793:Babesia bovis / Q000378:metabolism* / Q000472:pathogenicity; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D015232:Dinoprostone / Q000235:genetics / Q000378:metabolism*; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D066292:Lipid Droplets / Q000502:physiology; D050356:Lipid Metabolism / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D051216:Toll-Like Receptor 6 / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D051216:Toll-Like Receptor 6; D016753:Interleukin-10; D051546:Cyclooxygenase 2; D015232:Dinoprostone
keywords: Babesia bovis; Lipids; Macrophages; TLR6