title: Preparation, degradation and in vitro release of ciprofloxacin-eluting ureteral stents for potential antibacterial application.
abstract: Drug-eluting stents with biodegradable polymers as reservoirs have shown great potential in the application of interventional therapy due to their capability of local drug delivery. Herein, poly(l-lactide-co-ε-caprolactone) (PLCL) with three different compositions as carriers for ciprofloxacin lactate (CIP) was coated on ureteral stents by the dipping method. To simulate a body environment, degradation behavior of PLCL as both the bulk film and the stent coating was evaluated in artificial urine (AU, pH6.20) respectively at 37°C for 120days by tracing their weight/Mn loss, water absorption and surface morphologies. Furthermore, the release profile of the eluting drug CIP on each stent exhibited a three-stage pattern, which was greatly affected by the degradation behavior of PLCL except for the burst stage. Interestingly, the degradation results on both macroscopic and molecular level indicated that the release mechanism at stage I was mainly controlled by chain scission instead of the weight loss or morphological changes of the coatings. While for stage II, the release profile was dominated by erosion resulting from the hydrolysis reaction autocatalyzed by acidic degradation residues. In addition, ciprofloxacin-loaded coatings displayed a significant bacterial resistance against E. coli and S. aureus without obvious cytotoxicity to Human foreskin fibroblasts (HFFs). Our results suggested that PLCL copolymers with tunable degradation rate as carriers for ciprofloxacin lactate could be used as a promising long-term antibacterial coating for ureteral stents.
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials and Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Ma
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials and Science and Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: yxiao@ecust.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Collaborative Innovation Center for Petrochemical New Materials, Anqing, Anhui 246011, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials and Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lei
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials and Science and Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing, 130 Meilong Road, Shanghai 200237, China. Electronic address: mdlang@ecust.edu.cn.
authors.forename: Meidong
authors.initials: M
authors.lastname: Lang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology / Q000652:urine; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002939:Ciprofloxacin / Q000737:chemistry* / Q000494:pharmacology / Q000652:urine; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D054855:Drug-Eluting Stents; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D011091:Polyesters; D002939:Ciprofloxacin; C062968:lactide-caprolactone copolymer
keywords: Antibacterial; Ciprofloxacin lactate; Coating; Cytotoxicity; Degradation; Release mechanism; Ureteral stents