title: Modulation of NADPH-oxidase gene expression in rolB-transformed calli of Arabidopsis thaliana and Rubia cordifolia.
abstract: Expression of rol genes from Agrobacterium rhizogenes induces reprogramming of transformed plant cells and provokes pleiotropic effects on primary and secondary metabolism. We have previously established that the rolB and rolC genes impair reactive oxygen species (ROS) generation in transformed cells of Rubia cordifolia and Arabidopsis thaliana. In the present investigation, we tested whether this effect is associated with changes in the expression levels of NADPH oxidases, which are considered to be the primary source of ROS during plant-microbe interactions. We identified two full-length NADPH oxidase genes from R. cordifolia and examined their expression in non-transformed and rolB-transformed calli. In addition, we examined the expression of their homologous genes from A. thaliana in non-transformed and rolB-expressing cells. The expression of Rboh isoforms was 3- to 7-fold higher in both R. cordifolia and A. thaliana rolB-transformed cells compared with non-transformed cells. Our results for the first time show that Agrobacterium rolB gene regulates particular NADPH oxidase isoforms.
authors.affiliation: Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia.
authors.forename: Galina
authors.initials: G
authors.lastname: Veremeichik
authors.affiliation: Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia; Far Eastern Federal University, Vladivostok, 690950, Russia.
authors.forename: Victor
authors.initials: V
authors.lastname: Bulgakov
authors.affiliation: Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia; Far Eastern Federal University, Vladivostok, 690950, Russia. Electronic address: yn80@mail.ru.
authors.forename: Yury
authors.initials: Y
authors.lastname: Shkryl
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D001426:Bacterial Proteins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D019255:NADPH Oxidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D045470:Rubia / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics; D014170:Transformation, Genetic; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; D019255:NADPH Oxidases; C092241:RolB protein, Agrobacterium rhizogenes; D001617:beta-Glucosidase
keywords: Agrobacterium rhizogenes; Arabidopsis thaliana; NADPH oxidases; Rubia cordifolia; rolB gene