title: [Effects of dexmedetomidine on microcirculatory perfusion in rabbits with renal ischemia/reperfusion injury: quantitative evaluation with contrast-enhanced ultrasound].
abstract: OBJECTIVE
  To investigate the effects of dexmedetomidine on renal microcirculatory perfusion in rabbits with renal ischemia/reperfusion (I/R) injury rabbit by quantitative analysis of contrast-enhanced ultrasound (CEUS).
  
  
  METHODS
  Twenty- four New Zealand rabbits were randomly divided into 3 groups (8 in each), including a control group, renal I/R injury group and dexmedetomidine group. In the latter two groups, the right kidney of the rabbits was resected and I/R injury was induced in the left kidney. In dexmedetomidine group, the rabbits received an intraperitoneal dose of 10 µg/kg dexmedetomidine 30 min before renal ischemia, and 24 h after reperfusion, the renal size and renal artery resistance (RI) were measured, and renal cortex perfusion was observed by CEUS. The time-to-peak intensity (TTP), peak signal intensity (PSI), gradient between start frame to peak frame (Grad) and area under the curve (AUC) were quantitatively analyzed using the time-intensity curves. Pathological changes of the kidney were also observed.
  
  
  RESULTS
  Compared with the control group, the rabbits in I/R and dexmedetomidine groups showed distinct changes of the renal size with obvious renal pathologies. RI, PPT and AUC all increased, and PSI and Grad decreased significantly in I/R and dexmedetomidine groups (P<0.05). Compared with I/R group, obvious improvement of the renal size and renal pathologies were observed in dexmedetomidine group, which showed significantly decreased RI, PPT and AUC and increased PSI and Grad (P<0.05).
  
  
  CONCLUSIONS
  CEUS combined with the time-intensity curve parameters allows quantitative and dynamic analysis of the protective effects of dexmedetomidine on microcirculatory perfusion in rabbits with renal I/R injury.
authors.affiliation: Department of Anesthesiology, Nanjing Hospital Affiliated to Nanjing Medical University/Nanjing First Hospital, Nanjing 210006, China.E-mail: siyanna@163.com.
authors.forename: Yan-Na
authors.initials: YN
authors.lastname: Si
authors.forename: Liu
authors.initials: L
authors.lastname: Han
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li-Hai
authors.initials: LH
authors.lastname: Chen
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Xu
authors.forename: Fan
authors.initials: F
authors.lastname: Sun
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Pan
authors.forename: Ling-Qing
authors.initials: LQ
authors.lastname: Zeng
authors.forename: Hong-Guang
authors.initials: HG
authors.lastname: Bao
mesh_terms: D000818:Animals; D020927:Dexmedetomidine / Q000494:pharmacology*; D004195:Disease Models, Animal; D007668:Kidney / Q000098:blood supply / Q000187:drug effects; D007674:Kidney Diseases / Q000188:drug therapy*; D008833:Microcirculation / Q000187:drug effects*; D011817:Rabbits; D012077:Renal Artery / Q000187:drug effects; D015427:Reperfusion Injury / Q000188:drug therapy*
chemical_list: D020927:Dexmedetomidine