title: Effective method for the detection of piroxicam in human plasma using HPLC.
abstract: Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used by the general population to alleviate inflammation and pain after oral surgeries. Piroxicam is among the most commonly used NSAIDs and excels in controlling pain, swelling, trismus and other common symptoms of inflammation. This study aimed to evaluate different concentrations of piroxicam and its major metabolite, 5'-hydroxypiroxicam, in human plasma samples over time using high performance liquid chromatography (HPLC) after liquid-liquid extraction. Briefly, 10 volunteers participated in this study after approval by the Ethics Committee of Bauru School of Dentistry, Universidade de São Paulo - USP, Brazil. Volunteers received a single dose oral of piroxicam (20 mg) and had blood collected at various times following an established protocol. The methodology of liquid-liquid extraction was effective for determining concentrations of piroxicam in plasma using HPLC in 10 out of 10 volunteers while 5'-hydroxypiroxicam was only detected in 2 out of 10 volunteers.
authors.affiliation: Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
authors.forename: Adriana Maria
authors.initials: AM
authors.lastname: Calvo
authors.affiliation: Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
authors.forename: Mariel Tavares de Oliveira
authors.initials: MT
authors.lastname: Prado
authors.affiliation: Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
authors.forename: Thiago José
authors.initials: TJ
authors.lastname: Dionísio
authors.affiliation: Universidade de São Paulo - USP, School of Pharmaceutical Sciences of Ribeirão Preto, Laboratory Specialist, Department of Clinical, Toxicologic and Bromatologic Analyses, Ribeirão Preto, SP, Brazil.
authors.forename: Maria Paula
authors.initials: MP
authors.lastname: Marques
authors.affiliation: Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
authors.forename: Daniel Thomas
authors.initials: DT
authors.lastname: Brozoski
authors.affiliation: Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
authors.forename: Vera Lúcia
authors.initials: VL
authors.lastname: Lanchote
authors.affiliation: Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
authors.forename: Flávio Augusto Cardoso
authors.initials: FA
authors.lastname: Faria
authors.affiliation: Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
authors.forename: Carlos Ferreira
authors.initials: CF
authors.lastname: Santos
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods*; D009288:Naproxen / Q000097:blood / Q000493:pharmacokinetics; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D012016:Reference Values; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010894:Piroxicam; D009288:Naproxen; C051024:5'-hydroxypiroxicam