title: Identification and Expression Profile of Two Putative Odorant-Binding Proteins from the Neotropical Brown Stink Bug, Euschistus heros (Fabricius) (Hemiptera: Pentatomidae).
abstract: This work presents the first identification of putative odorant-binding proteins (OBPs) from a member of the Pentatomidae, i.e. the brown stink bug Euschistus heros (Fabricius), an important pest of soybean in Brazil. Antennae from both sexes of E. heros adults (12 days old and unmated) were used to construct a cDNA library, from which two transcripts encoding putative E. heros OBPs (EherOBPs) were identified. The expression levels of EherOBP1 and EherOBP2 were found to be higher in male antennae than in female and there was difference in expression in legs, wings, and abdomens of the two sexes. The histolocalization of EherOBP1 and EherOBP2 transcripts in antennae also showed a sexual dimorphism in the chemoreception system, with different expression sites in the antennal segments between males and females, occurring predominantly at the base of the sensillum. The implications of these findings for stink bug chemoreception are discussed.
authors.affiliation: Instituto de Ciências Biológicas, Univ de Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, Brasil. fariaslr@gmail.com.|Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil. fariaslr@gmail.com.
authors.forename: L R
authors.initials: LR
authors.lastname: Farias
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil.
authors.forename: D P
authors.initials: DP
authors.lastname: Paula
authors.affiliation: Dept of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, UK.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhou
authors.affiliation: Dept of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, UK.
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil.
authors.forename: G J
authors.initials: GJ
authors.lastname: Pappas
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil.
authors.forename: M C B
authors.initials: MC
authors.lastname: Moraes
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil.
authors.forename: R A
authors.initials: RA
authors.lastname: Laumann
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Borges
authors.affiliation: Dept of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Birkett
authors.affiliation: Dept of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Pickett
authors.affiliation: Dept of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, UK.
authors.forename: L M
authors.initials: LM
authors.lastname: Field
authors.affiliation: Instituto de Ciências Biológicas, Univ de Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, Brasil.
authors.forename: S N
authors.initials: SN
authors.lastname: Báo
mesh_terms: D000818:Animals; D001938:Brazil; D005260:Female; D020062:Heteroptera / Q000737:chemistry*; D008297:Male; D018035:Receptors, Odorant / Q000032:analysis* / Q000378:metabolism; D013025:Glycine max
chemical_list: D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Antennae; amphipathic proteins; chemoreception; olfaction; semiochemicals; sensillum