title: Preliminary survey of local bacteriophages with lytic activity against multi-drug resistant bacteria.
abstract: Bacteriophages (phages) represent a potential alternative for combating multi-drug resistant bacteria. Because of their narrow host range and the ever emergence of novel pathogen variants the continued search for phages is a prerequisite for optimal treatment of bacterial infections. Here we performed an ad hoc survey in the surroundings of a University hospital for the presence of phages with therapeutic potential. To this end, 16 aquatic samples of different origins and locations were tested simultaneously for the presence of phages with lytic activity against five current, but distinct strains each from the ESKAPE-group (i.e., Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter cloacae). Phages could be isolated for 70% of strains, covering all bacterial species except S. aureus. Apart from samples from two lakes, freshwater samples were largely devoid of phages. By contrast, one liter of hospital effluent collected at a single time point already contained phages active against two-thirds of tested strains. In conclusion, phages with lytic activity against nosocomial pathogens are unevenly distributed across environments with the prime source being the immediate hospital vicinity.
authors.affiliation: Division of Virology, Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Latz
authors.affiliation: Division of Virology, Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: Adam
authors.initials: A
authors.lastname: Wahida
authors.affiliation: Division of Virology, Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: Assuda
authors.initials: A
authors.lastname: Arif
authors.affiliation: Department of Infection Control and Infectious Diseases, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: Helga
authors.initials: H
authors.lastname: Häfner
authors.affiliation: Electron Microscopy Facility, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Hoß
authors.affiliation: Division of Virology, Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Ritter
authors.affiliation: Division of Virology, Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany. hhorz@ukaachen.de.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Horz
mesh_terms: D040981:Acinetobacter baumannii / Q000821:virology*; D001435:Bacteriophages / Q000302:isolation & purification*; D024901:Drug Resistance, Multiple, Bacterial; D016972:Enterobacter cloacae / Q000821:virology*; D016984:Enterococcus faecium / Q000821:virology*; D058507:Host Specificity; D007711:Klebsiella pneumoniae / Q000821:virology*; D000071059:Phage Therapy / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D013211:Staphylococcus aureus / Q000821:virology*; D062065:Wastewater / Q000821:virology
chemical_list: D062065:Wastewater
keywords: Bacteriophages phage therapy; ESKAPE; Multi-drug resistance; Phage isolation