title: TheVibrio cholerae ToxRRegulon Encodes Host-Specific Chemotaxis Proteins that Function in Intestinal Colonization.
abstract: Virulence gene regulation inVibrio choleraeis under the control of theToxR-ToxT regulatory cascade. Chemotaxis and net motility have been shown to influence the infectivity ofVibrio cholerae. V. cholerae toxRmutants do not synthesize proteins required for chemotaxis towards mucus. The inability of thetoxRmutant strain to recognize and swim towards mucus is due to their failure to synthesize AcfB, a methyl-accepting chemotaxis protein. AcfB has previously been shown to be involved in intestinal colonization using the infant mouse model of cholera infection. Wild typeV. choleraerecognizes galactose-6-sulfate in the capillary tube assay whereasV. cholerae acfBmutants fail to migrate into the capillary tubes. Vibrio strains carrying a mutation intcpI, aToxRregulated gene found within the Vibrio Pathogenicity Island (VPI), which encodes a methyl accepting chemotaxis protein are fully chemotactic towards mucus and galactose-6-sulfate.
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, United States.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Selvaraj
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, United States.
authors.forename: Rohit
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, United States.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Peterson
keywords: Accessory colonization factor; Chemotaxis; ToxR Regulon; Vibrio cholerae