title: Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae).
abstract: Ionotropic receptors (IRs) mainly detect the acids and amines having great importance in many insect species, representing an ancient olfactory receptor family in insects. In the present work, we performed RNAseq of Microplitis mediator antennae and identified seventeen IRs. Full-length MmedIRs were cloned and sequenced. Phylogenetic analysis of the Hymenoptera IRs revealed that ten MmedIR genes encoded "antennal IRs" and seven encoded "divergent IRs". Among the IR25a orthologous groups, two genes, MmedIR25a.1 and MmedIR25a.2, were found in M. mediator. Gene structure analysis of MmedIR25a revealed a tandem duplication of IR25a in M. mediator. The tissue distribution and development specific expression of the MmedIR genes suggested that these genes showed a broad expression profile. Quantitative gene expression analysis showed that most of the genes are highly enriched in adult antennae, indicating the candidate chemosensory function of this family in parasitic wasps. Using immunocytochemistry, we confirmed that one co-receptor, MmedIR8a, was expressed in the olfactory sensory neurons. Our data will supply fundamental information for functional analysis of the IRs in parasitoid wasp chemoreception.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Shan-Ning
authors.initials: SN
authors.lastname: Wang
authors.affiliation: College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding, Hebei 071000, China.
authors.forename: Zi-Yun
authors.initials: ZY
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Department of Entomology, Faculty of Crop Protection, Sindh Agriculture University Tandojam, Pakistan.
authors.forename: Khalid Hussain
authors.initials: KH
authors.lastname: Dhiloo
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Shan
authors.affiliation: College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China.
authors.forename: Rui-Jun
authors.initials: RJ
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yjzhang@ippcaas.cn.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Guo
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009036:Moths / Q000469:parasitology; D018034:Olfactory Receptor Neurons / Q000378:metabolism; D016133:Polymerase Chain Reaction; D058468:Receptors, Ionotropic Glutamate / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D059467:Transcriptome; D014863:Wasps / Q000235:genetics* / Q000502:physiology
chemical_list: D019476:Insect Proteins; D058468:Receptors, Ionotropic Glutamate
keywords: Antennae; Expression profile; Ionotropic receptor; Microplitis mediator; Olfactory sensory neuron