title: Rebalance between 7S and 11S globulins in soybean seeds of differing protein content and 11SA4.
abstract: Protein content and globulin subunit composition of soybean seeds affect the quality of soy foods. In this proteomic study, the protein profile of soybean seeds with high (∼45.5%) or low (∼38.6%) protein content and with or without the glycinin (11S) subunit 11SA4 was examined. 44 unique proteins and their homologues were identified and showed that both protein content and 11SA4 influenced the abundance of a number of proteins. The absence of 11SA4 exerted a greater impact than the protein content, and led to a decreased abundance of glycinin G2/A2B1 and G5/A5A4B3 subunits, which resulted in lower total 11S with a concomitant higher total β-conglycinin (7S). Low protein content was associated with higher glycinin G3/A1aB1b and lower glycinin G4/A5A4B3. Using the proteomic approach, it was demonstrated that 11SA4 deficiency induced compensatory accumulation of 7S globulins and led to a similar total abundance for 7S+11S irrespective of protein content or 11SA4.
authors.affiliation: CSIRO Agriculture, 306 Carmody Road, St Lucia, QLD 4067, Australia. Electronic address: Aijun.Yang@csiro.au.
authors.forename: A
authors.initials: A
authors.lastname: Yang
authors.affiliation: CSIRO Agriculture, 306 Carmody Road, St Lucia, QLD 4067, Australia; College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.affiliation: CSIRO Agriculture, 306 Carmody Road, St Lucia, QLD 4067, Australia; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: A
authors.initials: A
authors.lastname: Zheng
authors.affiliation: CSIRO Agriculture, 306 Carmody Road, St Lucia, QLD 4067, Australia.
authors.forename: A T
authors.initials: AT
authors.lastname: James
mesh_terms: D005916:Globulins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D045730:Soy Foods / Q000032:analysis; D030262:Soybean Proteins / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D005916:Globulins; D010940:Plant Proteins; D030262:Soybean Proteins; C011474:glycinin
keywords: 11SA4; Globulins subunits; Proteomics; Soybean protein content