title: The oxidative modification of cellular fibrin-stabilizing factor.
abstract: For the first time, the induced oxidative modification of cellular fibrin-stabilizing factor (cFXIII) has been studied. According to the electrophoresis analysis, the conversion of oxidized cFXIII into FXIIIa resulted in producing the enzyme that significantly lost the initial enzymatic activity. At the same time, FXIIIa subjected to induced oxidation was completely devoid of enzymatic activity. The results of FTIR spectroscopy showed that the oxidation of cFXIII or FXIIIa was accompanied by profound changes both in chemical and spatial structures of the protein. The results of this study are in good agreement with our earlier assumption regarding the antioxidant role of the regulatory subunits B of plasma fibrin-stabilizing factor.
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia. markrosenfeld@rambler.ru.
authors.forename: M A
authors.initials: MA
authors.lastname: Rosenfeld
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Shchegolikhin
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: V B
authors.initials: VB
authors.lastname: Leonova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Kostanova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: M I
authors.initials: MI
authors.lastname: Biryukova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Bychkova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: M L
authors.initials: ML
authors.lastname: Konstantinova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Vasilyeva
mesh_terms: D002118:Calcium / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005176:Factor XIII / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D010126:Ozone / Q000737:chemistry; D010920:Placenta / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011247:Pregnancy; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002413:Cations, Divalent; D016877:Oxidants; D011108:Polymers; D010126:Ozone; D005176:Factor XIII; D002118:Calcium