title: MicroRNA-126-3p suppresses cell proliferation by targeting PIK3R2 in Kaposi's sarcoma cells.
abstract: Kaposi's sarcoma is a highly vascular tumor of lymphatic endothelial origin. Many deregulated miRNAs, including miR-126-3p, have been identified in Kaposi's sarcoma tissues. miR-126-3p is the most highly endothelial-specific miRNA that regulates vascular integrity and angiogenesis. In this study, we aimed to determine the effect of miR-126-3p on Kaposi's sarcoma cells through transfection of a miRNA mimic and inhibitor. Moreover, we searched the target gene (PIK3R2) of miR-126-3p using bioinformatics software and further verified PIK3R2 using luciferase reporter assays, Real-time quantitative PCR (qRT-PCR) and western blot. The results demonstrated that miR-126-3p inhibited cell proliferation, arrested cell cycle progression, induced cell apoptosis, and inhibited cell invasion of SLK cells. The bioinformatics analysis and luciferase reporter assay revealed that PIK3R2 mRNA is a direct target of miR-126-3p. Moreover, the level of expression of the PIK3R2 gene was downregulated in SLK cells transfected with miR-126-3p siRNAs. Therefore, our data demonstrated that miR-126-3p is a tumor suppressor miRNA that acts by targeting PIK3R2 in Kaposi's sarcoma cells. These findings contribute to our understanding of the molecular mechanisms underlying Kaposi's sarcoma.
authors.affiliation: Department of Dermatology and Venereology, People's Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China.
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Wu
authors.affiliation: Clinical Medical Research Center, People's Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China.
authors.forename: Zong-Feng
authors.initials: ZF
authors.lastname: Zhao
authors.affiliation: Department of Dermatology and Venereology, People's Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Kang
authors.affiliation: Department of Dermatology and Venereology, People's Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: Department of Dermatology and Venereology, People's Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Dermatology and Venereology, People's Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China.
authors.forename: Xiong-Ming
authors.initials: XM
authors.lastname: Pu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases; C560566:phosphoinositol-3 kinase regulatory subunit 2, human
keywords: Kaposi’s sarcoma; PIK3R2; miR-126-3p; suppress; target