title: RNA- and protein-mediated control of Listeria monocytogenes virulence gene expression.
abstract: The model opportunistic pathogen Listeria monocytogenes has been the object of extensive research, aiming at understanding its ability to colonize diverse environmental niches and animal hosts. Bacterial transcriptomes in various conditions reflect this efficient adaptability. We review here our current knowledge of the mechanisms allowing L. monocytogenes to respond to environmental changes and trigger pathogenicity, with a special focus on RNA-mediated control of gene expression. We highlight how these studies have brought novel concepts in prokaryotic gene regulation, such as the 'excludon' where the 5'-UTR of a messenger also acts as an antisense regulator of an operon transcribed in opposite orientation, or the notion that riboswitches can regulate non-coding RNAs to integrate complex metabolic stimuli into regulatory networks. Overall, the Listeria model exemplifies that fine RNA tuners act together with master regulatory proteins to orchestrate appropriate transcriptional programmes.
authors.affiliation: a École Normale Supérieure , PSL Research University, CNRS, Inserm, Institut de Biologie de l'École Normale Supérieure (IBENS), Équipe Infection et Devenir de l'ARN , Paris , France.|b INRA, IBENS , Paris , France.
authors.forename: Alice
authors.initials: A
authors.lastname: Lebreton
authors.affiliation: c Institut Pasteur, Unité des Interactions Bactéries-Cellules , Paris , France.|d Inserm , Paris , France.|e INRA, USC2020 , Paris , France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Cossart
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D059647:Gene-Environment Interaction; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics* / Q000472:pathogenicity*; D051379:Mice; D010454:Peptide Termination Factors / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins / Q000378:metabolism; D058928:Riboswitch; D059467:Transcriptome / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D010454:Peptide Termination Factors; C545356:PrfA protein, Listeria monocytogenes; D012329:RNA, Bacterial; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; D058928:Riboswitch
keywords: CRISPR; excludon; non-coding RNA; riboswitch; thermosensor; transcriptome