title: Alterations in triglyceride rich lipoproteins are related to endothelial dysfunction in metabolic syndrome.
abstract: Our aim was to analyze the effect of circulating triglyceride rich lipoprotein (TRL) on endothelial function in metabolic syndrome (MetS).
  
  
  METHODS
  We studied 40 patients with MetS (ATPIII), divided into those presenting normal endothelial function (n=19) and those with endothelial dysfunction (n=21) by means of the evaluation of pulse wave velocity, before and after brachial artery ischemia. In fasting serum we measured lipid and lipoprotein profile, insulin and glucose (HOMA-IR). Moreover, isolated TRL (d<1006g/l) were chemically characterized. In parallel, using randomly selected TRL from MetS patients with endothelial dysfunction (n=6) and MetS patients with normal endothelial function (n=6), the ability of TRL to inhibit ACh-induced vasorelaxation (10(-9)-10(-5)mM) on aortic rings previously pre-contracted by noradrenaline (10(-8)mM) was evaluated.
  
  
  RESULTS
  Interestingly, TRL isolated from MetS patients presenting endothelial dysfunction showed triglyceride over-enrichment (59.1±4.8 vs. 54.1±4.7%; p=0.04), even after adjusting by potential confounders (p=0.05). In addition, while TRL resulting from both MetS groups significantly inhibited endothelium dependent vasorelaxation (p<0.001), TRL from MetS patients with endothelial dysfunction showed a strong tendency to a greater inhibition of vasorelaxation (p=0.06). Moreover, TRL-triglyceride (%) showed a strong tendency to correlate with the grade of vasorelaxation inhibition exerted by TRL (r=0.60; p=0.05).
  
  
  CONCLUSIONS
  These results, taken together, would allow inferring for the first time that the predominance of triglyceride over-enriched TRL in circulation in MetS would induce endothelial dysfunction, contributing to the inherent cardiovascular risk of MetS.
authors.affiliation: Laboratory of Lipids and Atherosclerosis, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Argentina. Electronic address: dmlucero@ffyb.uba.ar.
authors.forename: Diego
authors.initials: D
authors.lastname: Lucero
authors.affiliation: Laboratory of Lipids and Atherosclerosis, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Argentina.
authors.forename: Graciela I
authors.initials: GI
authors.lastname: López
authors.affiliation: Pharmacology Department, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
authors.forename: Susana
authors.initials: S
authors.lastname: Gorzalczany
authors.affiliation: Arterial Hypertension Laboratory, Cardiology Division, University Hospital of Buenos Aires, University of Buenos Aires, Argentina.
authors.forename: Mariano
authors.initials: M
authors.lastname: Duarte
authors.affiliation: Gastroenterology Division, University Hospital of Buenos Aires, University of Buenos Aires, Argentina.
authors.forename: Esteban
authors.initials: E
authors.lastname: González Ballerga
authors.affiliation: Gastroenterology Division, University Hospital of Buenos Aires, University of Buenos Aires, Argentina.
authors.forename: Juan
authors.initials: J
authors.lastname: Sordá
authors.affiliation: Laboratory of Lipids and Atherosclerosis, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Schreier
authors.affiliation: Laboratory of Lipids and Atherosclerosis, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Argentina.
authors.forename: Valeria
authors.initials: V
authors.lastname: Zago
mesh_terms: D015415:Biomarkers / Q000097:blood*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D024821:Metabolic Syndrome / Q000150:complications*; D008875:Middle Aged; D011379:Prognosis; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood*; D014652:Vascular Diseases / Q000097:blood / Q000175:diagnosis* / Q000209:etiology
chemical_list: D015415:Biomarkers; D008055:Lipids; D008074:Lipoproteins; D014280:Triglycerides; C030087:lipoprotein triglyceride
keywords: Endothelial dysfunction; Endothelium; Metabolic syndrome; Triglyceride rich lipoprotein