title: Pterostilbene Is a Potential Candidate for Control of Blackleg in Canola.
abstract: Two stilbenes, resveratrol and pterostilbene, exhibit antifungal activity against Leptosphaeria maculans, the fungal pathogen responsible for blackleg (stem canker) in canola (Brassica napus). In vitro studies on the effect of these stilbenes on L. maculans mycelial growth and conidia germination showed that pterostilbene is a potent fungicide and sporicide, but resveratrol only exerted minor inhibition on L. maculans. Cell viability of hyphae cultures was markedly reduced by pterostilbene and SYTOX green staining showed that cell membrane integrity was compromised. We demonstrate that pterostilbene exerts fungicidal activity across 10 different L. maculans isolates and the compound confers protection to the blackleg-susceptible canola cv. Westar seedlings. The potential of pterostilbene as a control agent against blackleg in canola is discussed.
authors.affiliation: Department of Economic Development, Jobs, Transport and Resources, Grains Innovation Park, 110 Natimuk Road, Horsham, VIC 3401, Australia.
authors.forename: Joshua C O
authors.identifier: http://orcid.org/0000-0002-3678-4789
authors.initials: JC
authors.lastname: Koh
authors.affiliation: Department of Economic Development, Jobs, Transport and Resources, Grains Innovation Park, 110 Natimuk Road, Horsham, VIC 3401, Australia.
authors.forename: Denise M
authors.initials: DM
authors.lastname: Barbulescu
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, VIC 3010, Australia.|Department of Economic Development, Jobs, Transport and Resources, AgriBio, Centre for AgriBioscience, 5 Ring Road, La Trobe University, Bundoora, VIC 3083, Australia.
authors.forename: Phil A
authors.initials: PA
authors.lastname: Salisbury
authors.affiliation: Department of Economic Development, Jobs, Transport and Resources, AgriBio, Centre for AgriBioscience, 5 Ring Road, La Trobe University, Bundoora, VIC 3083, Australia.
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Slater
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D029688:Brassica napus / Q000187:drug effects* / Q000382:microbiology; D002478:Cells, Cultured; D005659:Fungicides, Industrial / Q000494:pharmacology; D018525:Germination / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D018547:Plant Stems / Q000187:drug effects; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D005659:Fungicides, Industrial; D013267:Stilbenes; C107773:pterostilbene