title: Delineating the antigenotoxic and anticytotoxic potentials of 4-methylimidazole against ethyl methanesulfonate toxicity in bone marrow cell of swiss albino mice.
abstract: 4-Methylimidazole (4-MEI) is mostly used in beverages and coloring food, dark beers and common brands of cola drinks, which may contain more than 100 μg of this compound per 12-ounce serving. This study was aimed to investigate the antigenotoxic and anticytotoxic effects of 4-MEI (100, 130 and 160 mg/kg) against ethyl methanesulfonate (240 mg/kg) using chromosome aberrations (CAs) and Mitotic index (MI) tests in bone marrow cells of Swiss Albino Mice at 12 h and 24 h treatment periods. So, the t-test was used for the statistical analysis.In this research, 4-MEI at all concentrations for 12 h treatment period reduced chromosomal aberrations and at 130 and 160 mg/kg concentrations for 24 h treatment period increased chromosomal aberrations induced by EMS (240 mg/kg), but th ese reductions and increases were not significant. Also, intraperitoneal injection of 4-MEI at doses of 100, 130 and 160 mg/kg combined with EMS (240 mg/kg) showed that the mitotic index was decreased at 100 and 130 mg/kg for 12h and 130 mg/kg for 24 h treatment periods, when compared to positive sample (EMS), but did not show any statistically difference from the EMS treated group. It can be concluded that 4-MEI might not be antigenotoxic and protective effects in bone marrow cells of Swiss Albino Mice, because 4-MEI could not reduce the chromosomal aberrations induced by EMS.
authors.forename: M
authors.initials: M
authors.lastname: Norizadeh Tazehkand
authors.forename: M
authors.initials: M
authors.lastname: Topaktas
authors.forename: M B
authors.initials: MB
authors.lastname: Yilmaz
authors.forename: O
authors.initials: O
authors.lastname: Hajipour
authors.forename: E
authors.initials: E
authors.lastname: Valipour
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002869:Chromosome Aberrations; D004249:DNA Damage / Q000187:drug effects*; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*
chemical_list: D007093:Imidazoles; D009153:Mutagens; D005020:Ethyl Methanesulfonate; C023439:4-methylimidazole
keywords: 4-Methylimidazole; anticytotoxicity; antigenotoxicity; chromosome aberration.; ethyl methansulfonate