title: Multiplex PCR assay underreports true bloodstream infections with coagulase-negative staphylococci in hematological patients with febrile neutropenia.
abstract: SeptiFast multiplex PCR assay was evaluated for detecting true bloodstream infections (BSIs) with coagulase-negative staphylococci (CoNS) in neutropenic hematological patients. Sensitivity for samples representing true CoNS-BSIs was 23.3% with an integrated cutoff and increased to 83.3% if the cutoff was neglected. Hence, the cutoff may prohibit timely targeted antimicrobial therapy.
authors.affiliation: Institute of Medical Microbiology, University Hospital Münster, Domagkstr. 10, 48149 Münster, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Reers
authors.affiliation: Institute of Medical Microbiology, University Hospital Münster, Domagkstr. 10, 48149 Münster, Germany.
authors.forename: Evgeny A
authors.initials: EA
authors.lastname: Idelevich
authors.affiliation: Institute of Medical Microbiology, University Hospital Münster, Domagkstr. 10, 48149 Münster, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Pätkau
authors.affiliation: Institute of Biostatistics and Clinical Research, University of Münster, Schmeddingstraße 56, 48149 Münster, Germany.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Sauerland
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Tafelski
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Irit
authors.initials: I
authors.lastname: Nachtigall
authors.affiliation: Department of Medicine A, University Hospital Münster, Albert-Schweitzer-Campus 1/A1, 48149 Münster, Germany.
authors.forename: Wolfgang E
authors.initials: WE
authors.lastname: Berdel
authors.affiliation: Institute of Medical Microbiology, University Hospital Münster, Domagkstr. 10, 48149 Münster, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Peters
authors.affiliation: Department of Medicine A, University Hospital Münster, Albert-Schweitzer-Campus 1/A1, 48149 Münster, Germany.
authors.forename: Gerda
authors.initials: G
authors.lastname: Silling
authors.affiliation: Institute of Medical Microbiology, University Hospital Münster, Domagkstr. 10, 48149 Münster, Germany. Electronic address: kbecker@uni-muenster.de.
authors.forename: Karsten
authors.initials: K
authors.lastname: Becker
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods; D003030:Coagulase / Q000032:analysis*; D064147:Febrile Neutropenia / Q000175:diagnosis* / Q000382:microbiology; D019337:Hematologic Neoplasms / Q000150:complications*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D003030:Coagulase
keywords: Bloodstream infections; Coagulase-negative staphylococci; Cutoff; Febrile neutropenia; Multiplex PCR