title: NEDD8 ultimate buster-1 regulates the abundance of TRF1 at telomeres by promoting its proteasomal degradation.
abstract: The human telomeric protein TRF1 negatively regulates telomere length by inhibiting the access of telomerase to telomeres. Here, we describe a novel function of NEDD8 ultimate buster-1 (NUB1) for regulating the levels of TRF1 at telomeres. NUB1 is a NEDD8-interacting protein, which down-regulates the NEDD8 conjugation system. We showed that NUB1 physically interacts with TRF1 and promotes its degradation by the proteasome in the absence of NEDD8 conjugation. We also demonstrated that TRF1 is conjugated to NEDD8, and that neddylated TRF1 is targeted to the proteasome for degradation in a NUB1-dependent manner. These data suggest that NUB1 participates in telomere maintenance by regulating the levels of TRF1 at telomeres through both NEDD8-dependent and NEDD8-independent pathways.
authors.affiliation: Department of Integrated Omics for Biomedical Science, Yonsei University, Seoul, Korea.
authors.forename: Yu Young
authors.initials: YY
authors.lastname: Jeong
authors.affiliation: Department of Integrated Omics for Biomedical Science, Yonsei University, Seoul, Korea.
authors.forename: Joonyoung
authors.initials: J
authors.lastname: Her
authors.affiliation: Department of Integrated Omics for Biomedical Science, Yonsei University, Seoul, Korea.|Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
authors.forename: In Kwon
authors.initials: IK
authors.lastname: Chung
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D000075687:NEDD8 Protein; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D059748:Proteolysis; D016615:Telomere / Q000235:genetics / Q000378:metabolism*; D035321:Telomeric Repeat Binding Protein 1 / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000075687:NEDD8 Protein; C082860:NEDD8 protein, human; C430242:NUB1 protein, human; D035321:Telomeric Repeat Binding Protein 1; D014157:Transcription Factors; D014452:Ubiquitins; D046988:Proteasome Endopeptidase Complex
keywords: NEDD8 ultimate buster-1; neddylation; proteasome; protein degradation; telomere; telomeric repeat binding factor 1