title: Aluminum resistance in wheat involves maintenance of leaf Ca(2+) and Mg(2+) content, decreased lipid peroxidation and Al accumulation, and low photosystem II excitation pressure.
abstract: The phytotoxic aluminum species (Al(3+)) is considered as the primary factor limiting crop productivity in over 40 % of world's arable land that is acidic. We evaluated the responses of two wheat cultivars (Triticum aestivum L.) with differential Al resistance, cv. Yecora E (Al-resistant) and cv. Dio (Al-sensitive), exposed to 0, 37, 74 and 148 μM Al for 14 days in hydroponic culture at pH 4.5. With increasing Al concentration, leaf Ca(2+) and Mg(2+) content decreased, as well as the effective quantum yield of photosystem II (PSII) photochemistry (Φ PSII ), while a gradual increase in leaf membrane lipid peroxidation, Al accumulation, photoinhibition (estimated as F v /F m ), and PSII excitation pressure (1 - q p ) occurred. However, the Al-resistant cultivar with lower Al accumulation, retained larger concentrations of Ca(2+) and Mg(2+) in the leaves and kept a larger fraction of the PSII reaction centres (RCs) in an open configuration, i.e. a higher ratio of oxidized to reduced quinone A (QA), than plants of the Al-sensitive cultivar. Four times higher Al concentration in the nutrient solution was required for Al-resistant plants (148 μM Al) than for Al-sensitive (37 μM Al), in order to establish the same closed RCs. Yet, the decline in photosynthetic efficiency in the cultivar Dio was not only due to closure of PSII RCs but also to a decrease in the quantum yield of the open RCs. We suggest that Al(3+) toxicity may be mediated by nutrient deficiency and oxidative stress, and that Al-resistance of the wheat cultivar Yecora E, may be due at least partially, from the decreased Al accumulation that resulted to decreased reactive oxygen species (ROS) formation. However, under equal internal Al accumulation (exposure Al concentration: Dio 74 μM, Yecora E 148 μM) that resulted to the same oxidative stress, the reduced PSII excitation pressure and the better PSII functioning of the Al-resistant cultivar was probably due to the larger concentrations of Ca(2+) and Mg(2+) in the leaves. We propose that the different sensitivities of wheat cultivars to Al(3+) toxicity can be correlated to differences in the redox state of QA. Thus, chlorophyll fluorescence measurements can be a promising tool for rapid screening of Al resistance in wheat cultivars.
authors.affiliation: Department of Botany, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.|Department of Biology, University of Crete, Voutes University Campus, 70013, Heraklion, Crete, Greece.
authors.forename: Julietta
authors.initials: J
authors.lastname: Moustaka
authors.affiliation: Institute of Food Technology, Hellenic Agricultural Organization-Demeter, 1 S. Venizelou str., 14123, Lycovrissi, Greece.
authors.forename: Georgia
authors.initials: G
authors.lastname: Ouzounidou
authors.affiliation: Division of Botany, Department of Biology, Faculty of Science, Istanbul University, 34134, Istanbul, Turkey.
authors.forename: Gülriz
authors.initials: G
authors.lastname: Bayçu
authors.affiliation: Department of Botany, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece. moustak@bio.auth.gr.|Division of Botany, Department of Biology, Faculty of Science, Istanbul University, 34134, Istanbul, Turkey. moustak@bio.auth.gr.
authors.forename: Michael
authors.initials: M
authors.lastname: Moustakas
mesh_terms: D000535:Aluminum / Q000378:metabolism / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008274:Magnesium / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D014908:Triticum / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D000535:Aluminum; D008274:Magnesium; D002118:Calcium
keywords: Chlorophyll fluorescence; Nutrient uptake; Oxidative stress; Quantum yield; Redox state; Triticum aestivum