title: Multilocus sequence typing of Campylobacter jejuni and Campylobacter coli isolates from poultry, cattle and humans in Nigeria.
abstract: OBJECTIVE
  To determine the genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from Nigeria and to identify the association between multilocus sequence types and hosts (poultry, cattle and humans).
  
  
  RESULTS
  Isolates were identified using multiplex PCR assays. Multilocus sequence typing (MLST) was used to determine the genetic diversity of 36 Camp. jejuni and 24 Camp. coli strains isolated from poultry, cattle and humans. Of the 36 Camp. jejuni genotyped, 21 sequence types (ST) were found, 9 (43%) were new while of the 24 Camp. coli isolates genotyped, 22 STs were identified with 14 (64%) being new. The most prevalent sequence type was ST1932 followed by ST1036 and ST607 while the prevalent clonal complexes were CC-828, CC-460 and CC-353.
  
  
  CONCLUSIONS
  Campylobacter isolates from Nigeria were found to be diverse with novel genotypes. There was overlap of CC-828, CC-460 and CC-353 between the poultry, cattle and human isolates. Genetic exchange was also detected in two of the Camp. coli isolates.
  
  
  CONCLUSIONS
  This study highlights the genetic diversity of Campylobacter strains in Nigeria, demonstrating that Camp. jejuni and Camp. coli isolates are diverse and have both local and global strains. The predominant sequence types and clonal complexes found in this study differ from other countries; this exemplifies that different predominant Campylobacter populations exist between countries.
authors.affiliation: Bacterial Research Division, National Veterinary Research Institute, Vom, Nigeria.
authors.forename: S
authors.initials: S
authors.lastname: Ngulukun
authors.affiliation: Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Nigeria.
authors.forename: S
authors.initials: S
authors.lastname: Oboegbulem
authors.affiliation: Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Germany.
authors.forename: G
authors.identifier: http://orcid.org/0000-0003-1931-2701
authors.initials: G
authors.lastname: Klein
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary*; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D058885:Multilocus Sequence Typing / Q000379:methods; D009549:Nigeria / Q000453:epidemiology; D011200:Poultry; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D015995:Prevalence
keywords: Campylobacter; Nigerian isolates; diversity; genotyping; molecular epidemiology; multilocus sequence typing