title: Antibacterial Activity of Silver Nanoparticle-Loaded Soft Contact Lens Materials: The Effect of Monomer Composition.
abstract: In the present work, the effect of monomer composition on silver nanoparticles' (SNPs) binding capacity of hydrogels was investigated and their antibacterial efficacy was evaluated.
  
  
  
  Three series of poly-hydroxyethyl methacrylate (HEMA) hydrogels were prepared using methacrylic acid (MAA), methacrylamide (MAAM), and 4-vinylpyridine (4VP) as co-monomers, and ethylene glycol dimethacrylate (EGDMA) as cross-linker. SNPs binding capacity of hydrogels was evaluated in different concentrations (2, 10, and 20 ppm). In vitro antibacterial activity of SNP-loaded hydrogels was studied against Pseudomonas aeruginosa (P. aeruginosa) isolated from patients' eyes. Then, inhibitory effect of hydrogels in biofilm formation was evaluated in the presence of Staphylococcus epidermidis (S. epidermidis) (DSMZ 3270).
  
  
  
  Our data indicated that poly(HEMA-co-MAA-co-EGDMA) had superior binding affinity for SNPs in comparison with other hydrogels. All SNP-loaded hydrogels demonstrated excellent antimicrobial effects at all times against P. aeruginosa and S. epidermidis after soaking in 10 and 20 ppm SNP suspensions. Scanning electron microscope (SEM) images revealed excellent inhibitory effect of SNPs against biofilm formation on the surface of the hydrogels.
  
  
  
  This study indicated the effect of monomer compositions in SNP loading capacity of poly(HEMA) hydrogels and antibacterial efficacy of SNP-loaded hydrogels against P. aeruginosa and S. epidermidis, but further in vivo evaluation is necessary.
authors.affiliation: a Student Research Committee (SRC ), Mashhad University of Medical Sciences , Mashhad , Iran.|b Targeted Drug Delivery Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Shayani Rad
authors.affiliation: c Department of Pharmaceutical Control , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Bahman
authors.initials: B
authors.lastname: Khameneh
authors.affiliation: d Biotechnology Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Sabeti
authors.affiliation: b Targeted Drug Delivery Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Mohajeri
authors.affiliation: d Biotechnology Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Bibi Sedigheh
authors.initials: BS
authors.lastname: Fazly Bazzaz
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D053758:Nanoparticles; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012834:Silver / Q000494:pharmacology*; D013212:Staphylococcus epidermidis / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: Antimicrobial effect; biofilm; hydrogels; poly-hydroxyethyl methacrylate; silver nanoparticles