title: Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection.
abstract: Nervous necrosis virus (NNV) is the causative agent of viral encephalopathy and retinopathy (VER), a neurological disease responsible for high mortality of fish species worldwide. Taking advantage of our established Chinese perch brain (CPB) cell line derived from brain tissues of Mandarin fish (Siniperca chuatsi), the susceptibility of CPB cell to Red-Spotted Grouper nervous necrosis virus (RGNNV) was evaluated. The results showed that RGNNV replicated well in CPB cells, resulting in cellular apoptosis. Moreover, the susceptibility of Mandarin fish to RGNNV was also evaluated. Abnormal swimming was observed in RGNNV-infected Mandarin fish. In addition, the cellular vacuolation and viral particles were also observed in brain tissues of RGNNV-infected Mandarin fish by Hematoxylin-eosin staining or electronic microscopy. The established RGNNV susceptible brain cell line from freshwater fish will pave a new way for the study of the pathogenicity and replication of NNV in the future.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China. tujiagang@mail.hzau.edu.cn.
authors.forename: Jiagang
authors.initials: J
authors.lastname: Tu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China. chenwenjie53@163.com.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Chen
authors.affiliation: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, Guangdong, China. fuxiaozhe-1998@163.com.
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Fu
authors.affiliation: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, Guangdong, China. lin9902057@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, Guangdong, China. chang-ouqin@126.com.
authors.forename: Ouqin
authors.initials: O
authors.lastname: Chang
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China. zhaolijuan4234@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China. lanjiangfeng@mail.hzau.edu.cn.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Lan
authors.affiliation: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, Guangdong, China. liningq@126.com.
authors.forename: Ningqiu
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China. linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology*; D005393:Fish Diseases / Q000821:virology*; D030381:Nodaviridae; D010471:Perches / Q000821:virology*; D012327:RNA Virus Infections; D013045:Species Specificity
keywords: CPB; Mandarin fish; NNV; brain cell line; susceptibility