title: Evaluation of a two-stage antibacterial hydrogel dressing for healing in an infected diabetic wound.
abstract: Various types of wound dressings have been used to treat complex infections in diabetes mellitus. This study is the first to evaluate the healing effects using a two-stage dressing in infected diabetic wounds. A two-stage antibacterial hydrogel dressing (two-stage dressing) was established with two time phases, an antibacterial phase and a drug release phase. We established each phase by using a swelling and rate of drug release test. These results suggested that the antimicrobial phase is activated as soon as the two-stage dressing attaches to the skin. The drugs in the drug release layer of the dressing were released to a greater extent than expected 20-36 h after attachment to the skin, likely due to extensive water absorption. Histological analysis and measurement of vascular endothelial growth factor expression through in vivo testing suggested that the benefits of a two-stage dressing include rapid antibacterial properties, sustained drug release, and promotion of wound healing through cell proliferation as compared with the traditional composite antibacterial hydrogel dressing. Further in vivo tests confirmed that separation of the antibacterial and drug-releasing properties, along with biocompatibility and rapid wound closure rates made two-stage dressings suitable for healing of infected wounds. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1808-1817, 2017.
authors.affiliation: Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226019, China.|Nantong Tongda Chemicals Safety Evaluation Center Co. Ltd., Nantong, Jiangsu, 226019, China.
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.affiliation: School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China.|Institute of Analytical Chemistry for Life Science, Nantong University, Nantong, Jiangsu, 226019, China.
authors.forename: Dong-Lin
authors.initials: DL
authors.lastname: Xia
authors.affiliation: Nantong Tongda Chemicals Safety Evaluation Center Co. Ltd., Nantong, Jiangsu, 226019, China.|School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China.
authors.forename: Yan-Pei
authors.initials: YP
authors.lastname: Chen
authors.affiliation: Nantong Tongda Chemicals Safety Evaluation Center Co. Ltd., Nantong, Jiangsu, 226019, China.|School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China.|Institute of Analytical Chemistry for Life Science, Nantong University, Nantong, Jiangsu, 226019, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Nantong Tongda Chemicals Safety Evaluation Center Co. Ltd., Nantong, Jiangsu, 226019, China.|Institute of Analytical Chemistry for Life Science, Nantong University, Nantong, Jiangsu, 226019, China.
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Wang
authors.affiliation: School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Shen
authors.affiliation: Nantong Tongda Chemicals Safety Evaluation Center Co. Ltd., Nantong, Jiangsu, 226019, China.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Hu
authors.affiliation: Nantong Tongda Chemicals Safety Evaluation Center Co. Ltd., Nantong, Jiangsu, 226019, China.|School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China.|Institute of Analytical Chemistry for Life Science, Nantong University, Nantong, Jiangsu, 226019, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Gu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001458:Bandages; D048909:Diabetes Complications / Q000382:microbiology / Q000628:therapy*; D003921:Diabetes Mellitus, Experimental / Q000382:microbiology / Q000628:therapy*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D014946:Wound Infection / Q000628:therapy*
chemical_list: D000900:Anti-Bacterial Agents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: antibacterial; diabetes; drug release; nano-Ag; two-stage dressing