title: Effects of ozone (O3) therapy on cisplatin-induced ototoxicity in rats.
abstract: The aim of this study is to investigate the effect of rectal ozone and intratympanic ozone therapy on cisplatin-induced ototoxicity in rats. Eighteen female Wistar albino rats were included in our study. External auditory canal and tympanic membrane examinations were normal in all rats. The rats were randomly divided into three groups. Initially, all the rats were tested with distortion product otoacoustic emissions (DPOAE), and emissions were measured normally. All rats were injected with 5-mg/kg/day cisplatin for 3 days intraperitoneally. Ototoxicy had developed in all rats, as confirmed with DPOAE after 1 week. Rectal and intratympanic ozone therapy group was Group 1. No treatment was administered for the rats in Group 2 as the control group. The rats in Group 3 were treated with rectal ozone. All the rats were tested with DPOAE under general anesthesia, and all were sacrificed for pathological examination 1 week after ozone administration. Their cochleas were removed. The outer hair cell damage and stria vascularis damage were examined. In the statistical analysis conducted, a statistically significant difference between Group 1 and Group 2 was observed in all frequencies according to the DPOAE test. In addition, between Group 2 and Group 3, a statistically significant difference was observed in the DPOAE test. However, a statistically significant difference was not observed between Group 1 and Group 3 according to the DPOAE test. According to histopathological scoring, the outer hair cell damage score was statistically significantly high in Group 2 compared with Group 1. In addition, the outer hair cell damage score was also statistically significantly high in Group 2 compared with Group 3. Outer hair cell damage scores were low in Group 1 and Group 3, but there was no statistically significant difference between these groups. There was no statistically significant difference between the groups in terms of stria vascularis damage score examinations. Systemic ozone gas therapy is effective in the treatment of cell damage in cisplatin-induced ototoxicity. The intratympanic administration of ozone gas does not have any additional advantage over the rectal administration.
authors.affiliation: Department of Otorhinolaryngology, Bakırköy Training and Research Hospital, Istanbul, Turkey.
authors.forename: Hasan Emre
authors.initials: HE
authors.lastname: Koçak
authors.affiliation: Department of Otorhinolaryngology, Bağcılar Training and Research Hospital, Istanbul, Turkey.
authors.forename: Ümit
authors.initials: Ü
authors.lastname: Taşkın
authors.affiliation: Department of Otorhinolaryngology, Bağcılar Training and Research Hospital, Istanbul, Turkey.
authors.forename: Salih
authors.initials: S
authors.lastname: Aydın
authors.affiliation: Department of Otorhinolaryngology, Bağcılar Training and Research Hospital, Istanbul, Turkey.
authors.forename: Mehmet Faruk
authors.initials: MF
authors.lastname: Oktay
authors.affiliation: Department of Pathology, Bağcılar Training and Research Hospital, Istanbul, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Altınay
authors.affiliation: Bağcılar Training and Research Hospital Experimental Surgical Research and Skill Improvement Training Center, Istanbul, Turkey.
authors.forename: Duygu Sultan
authors.initials: DS
authors.lastname: Çelik
authors.affiliation: Hospitalist Hospital, Bağcılar, İstanbul, Turkey. kadiryucebas@gmail.com.
authors.forename: Kadir
authors.initials: K
authors.lastname: Yücebaş
authors.affiliation: Department of Otorhinolaryngology, Bağcılar Training and Research Hospital, Istanbul, Turkey.
authors.forename: Bengül
authors.initials: B
authors.lastname: Altaş
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects / Q000473:pathology; D005260:Female; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000473:pathology; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D011897:Random Allocation; D017208:Rats, Wistar; D013316:Stria Vascularis / Q000187:drug effects / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D010126:Ozone; D002945:Cisplatin
keywords: Cisplatin; Otoacoustic emission; Ototoxicy; Ozone