title: Association of G894T eNOS, 4G/5G PAI and T1131C APOA5 polymorphisms with susceptibility to myocardial infarction in Morocco.
abstract: BACKGROUND
  Myocardial infarction (MI) is a common multifactorial disease. Numerous studies have found that genetic plays an essential role in MI occurrence. The main objective of our case-control study is to explore the association of G894T eNOS (rs1799983), 4G/5G PAI (rs1799889) and T1131C APOA5 (rs662799) polymorphisms with MI susceptibility in the Moroccan population.
  
  
  RESULTS
  118 MI patients were recruited vs 184 healthy controls. DNA samples were genotyped by PCR-RFLP method using MboI, BslI and MseI restriction enzymes respectively for the G894T eNOS, 4G/5G PAI and T1131C APOA5 polymorphisms. Our results show that the G894T eNOS was significantly associated with increased risk of MI under the three genetic transmission models (dominant: OR = 1.64, 95% CI = 1.05-2.58, P = 0.003; recessive: OR = 2.15, 95% CI = 0.74-6.16, P = 0.03; additive: OR = 1.54, 95% CI = 1.06-2.23, P = 0.001). The T1131C APOA5 polymorphism was associated to MI risk in recessive and additive models (OR = 1.53, 95% CI = 0.72-3.2, P = 0.04 and OR = 1.78, 95% CI = 1.26-2.51, P = 0.03 respectively). For the 4G/5G PAI variant, even the cases and controls groups were not in Hardy-Weinberg Equilibrium (HWE), the dominant and additive models show a statistically significant association with MI risk (OR = 7.96, 95%CI = 3.83-16.36, P = 0.01 and OR = 1.96, 95% CI = 1.4-2.72, P = 0.03 respectively).
  
  
  CONCLUSIONS
  Our results suggest that G894T eNOS and T1131C APOA5 polymorphisms may be considered as genetic markers of MI among the Moroccan population. Further studies including larger sample sizes and exploring more genetic associations are needed to confirm our results and to better understand the susceptibility to MI.
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca, Morocco.
authors.forename: Hind
authors.initials: H
authors.lastname: Hassani Idrissi
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca, Morocco.
authors.forename: Wiam
authors.initials: W
authors.lastname: Hmimech
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca, Morocco.
authors.forename: Brehima
authors.initials: B
authors.lastname: Diakite
authors.affiliation: Department of Cardiology, University Hospital Center Ibn Rochd, Casablanca, Morocco.
authors.forename: Farah
authors.initials: F
authors.lastname: Korchi
authors.affiliation: Department of Cardiology, University Hospital Center Ibn Rochd, Casablanca, Morocco.
authors.forename: Dalila
authors.initials: D
authors.lastname: Baghdadi
authors.affiliation: Department of Cardiology, University Hospital Center Ibn Rochd, Casablanca, Morocco.
authors.forename: Rachida
authors.initials: R
authors.lastname: Habbal
authors.affiliation: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca, Morocco.
authors.forename: Sellama
authors.initials: S
authors.lastname: Nadifi
keywords: 4G/5G PAI; G894T eNOS; Moroccan population; Myocardial infarction; Susceptibility; T1131C APOA5