title: Mesoporous carbon-containing voltammetric biosensor for determination of tyramine in food products.
abstract: A voltammetric biosensor based on tyrosinase (TYR) was developed for determination of tyramine. Carbon material (multi-walled carbon nanotubes or mesoporous carbon CMK-3-type), polycationic polymer-i.e., poly(diallyldimethylammonium chloride) (PDDA), and Nafion were incorporated into titania dioxide sol (TiO2) to create an immobilization matrix. The features of the formed matrix were studied by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The analytical performance of the developed biosensor was evaluated with respect to linear range, sensitivity, limit of detection, long-term stability, repeatability, and reproducibility. The biosensor exhibited electrocatalytic activity toward tyramine oxidation within a linear range from 6 to 130 μM, high sensitivity of 486 μA mM(-1) cm(-2), and limit of detection of 1.5 μM. The apparent Michaelis-Menten constant was calculated to be 66.0 μM indicating a high biological affinity of the developed biosensor for tyramine. Furthermore, its usefulness in determination of tyramine in food product samples was also verified. Graphical abstract Different food samples were analyzed to determine tyramine using biosensor based on tyrosinase.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow, Poland. kochana@chemia.uj.edu.pl.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kochana
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Wapiennik
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Knihnicki
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Pollap
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow, Poland.
authors.forename: Paula
authors.initials: P
authors.lastname: Janus
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Oszajca
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kuśtrowski
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014439:Tyramine / Q000032:analysis* / Q000737:chemistry
chemical_list: D002244:Carbon; D014439:Tyramine
keywords: Biosensor; Food; Mesoporous carbon; Tyramine; Tyrosinase; Voltammetry