title: Thermosensitive magnetic liposomes with doxorubicin cell-penetrating peptides conjugate for enhanced and targeted cancer therapy.
abstract: To specifically deliver cytotoxic drug to tumor cells and enhance cellular uptake is the key for effective cancer therapy. In this paper, we described a novel drug targeting system, which is designed to combine features of biological (cell-penetrating peptides, CPPs) and physical (magnetic) drug targeting for use in the magnetic hyperthermia-triggered release. A doxorubicin-CPPs conjugate (DOX-CPPs) was loaded into thermosensitive magnetic liposomes (TSMLs) (DOX-CPPs/TSMLs), and in vitro DOX-CPPs thermosensitive release activity, anti-proliferation effect, in vivo targeted delivery as well as in vivo antitumor activity were determined. The results demonstrated that the DOX-CPPs/TSMLs showed good physicochemical properties, effective anti-proliferation effect in MCF-7 cells in vitro. Additionally, in vivo study, DOX-CPPs/TSMLs under AC magnetic field displayed superior in vivo targeted delivery efficacy, antitumor efficacy in an MCF-7 xenograft murine model. In conclusion, the application of DOX-CPPs/TSMLs under AC magnetic field may provide a strategy for the selective and efficient delivery of drug.
authors.affiliation: a Department of Clinical Laboratory , Huangshi Love & Health Hospital of Hubei Province , Huangshi , P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Lin
authors.affiliation: b Department of Pharmacy , Wuhan General Hospital of Guangzhou Military Command , Wuhan , P.R. China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Xie
authors.affiliation: c Beijing Key Laboratory of Drug Delivery Technology and Novel Formulation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , P.R. China , and.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: b Department of Pharmacy , Wuhan General Hospital of Guangzhou Military Command , Wuhan , P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Fu
authors.affiliation: b Department of Pharmacy , Wuhan General Hospital of Guangzhou Military Command , Wuhan , P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: d Beijing Institute of Pharmacology and Toxicology , Beijing , P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a Department of Clinical Laboratory , Huangshi Love & Health Hospital of Hubei Province , Huangshi , P.R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Deng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000008:administration & dosage* / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude
chemical_list: D000970:Antineoplastic Agents; D057846:Cell-Penetrating Peptides; D008081:Liposomes; D004317:Doxorubicin
keywords: Cell-penetrating peptides; durg delivery; liposomes; magnetic guidance; thermosensitive release