title: Reduced graphene oxide-nickel nanoparticles/biopolymer composite films for the sub-millimolar detection of glucose.
abstract: Hybrid conjugates of graphene with metallic/semiconducting nanostructures can improve the sensitivity of electrochemical sensors due to their combination of well-balanced electrical/electrocatalytic properties and superior surface-to-volume ratio. In this study, the synthesis and physical characterization of a hybrid conjugate of reduced graphene oxide and nickel nanoparticles (rGO-Ni NPs) is presented. The conjugate was further deposited onto a glassy carbon electrode as a nanocomposite film of chitosan and glucose oxidase. The electrochemical response and morphology of the films were investigated using SEM, CV, and EIS, and their applications as a glucose biosensor explored for the first time in proof-of-concept tests. The low operating potential along with the good linearity and sensitivity (up to 129 μA cm(-2) mM(-1)) found in the sub-millimolar range suggest potential applications in the self-management of hypoglycemia from blood samples or in the development of non-invasive assays for body fluids such as saliva, tears or breath.
authors.affiliation: Centre for Research in Chemistry of The University of Porto (CIQUP), Department of Chemistry and Biochemistry, Faculty of Sciences, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal. jpina@fc.up.pt and Centre for Mechanical Technology and Automation (TEMA), Department of Mechanical Engineering, University of Aveiro, 3810-193, Aveiro, Portugal and IFIMUP-IN and Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
authors.forename: Rahul
authors.initials: R
authors.lastname: Krishna
authors.affiliation: Centre for Research in Chemistry of The University of Porto (CIQUP), Department of Chemistry and Biochemistry, Faculty of Sciences, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal. jpina@fc.up.pt.
authors.forename: José M
authors.initials: JM
authors.lastname: Campiña
authors.affiliation: Centre for Research in Chemistry of The University of Porto (CIQUP), Department of Chemistry and Biochemistry, Faculty of Sciences, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal. jpina@fc.up.pt.
authors.forename: Paula M V
authors.initials: PM
authors.lastname: Fernandes
authors.affiliation: IFIMUP-IN and Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Ventura
authors.affiliation: Centre for Mechanical Technology and Automation (TEMA), Department of Mechanical Engineering, University of Aveiro, 3810-193, Aveiro, Portugal.
authors.forename: Elby
authors.initials: E
authors.lastname: Titus
authors.affiliation: Centre for Research in Chemistry of The University of Porto (CIQUP), Department of Chemistry and Biochemistry, Faculty of Sciences, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal. jpina@fc.up.pt.
authors.forename: António F
authors.initials: AF
authors.lastname: Silva
mesh_terms: D015374:Biosensing Techniques; D048271:Chitosan; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006108:Graphite; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites; D009532:Nickel; D010087:Oxides
chemical_list: D010087:Oxides; D006108:Graphite; D009532:Nickel; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose