title: Transcriptomic Analysis of Differentially Expressed Genes During Larval Development of Rapana venosa by Digital Gene Expression Profiling.
abstract: During the life cycle of shellfish, larval development, especially metamorphosis, has a vital influence on the dynamics, distribution, and recruitment of natural populations, as well as seed breeding. Rapana venosa, a carnivorous gastropod, is an important commercial shellfish in China, and is an ecological invader in the United States, Argentina, and France. However, information about the mechanism of its early development is still limited, because research in this area has long suffered from a lack of genomic resources. In this study, 15 digital gene expression (DGE) libraries from five developmental stages of R. venosa were constructed and sequenced on the IIIumina Hi-Sequation 2500 platform. Bioinformaticsanalysis identified numerous differentially and specifically expressed genes, which revealed that genes associated with growth, nervous system, digestive system, immune system, and apoptosis participate in important developmental processes. The functional analysis of differentially expressed genes was further implemented by gene ontology, and Kyoto encyclopedia of genes and genomes enrichment. DGE profiling provided a general picture of the transcriptomic activities during the early development of R. venosa, which may provide interesting hints for further study. Our data represent the first comparative transcriptomic information available for the early development of R. venosa, which is a prerequisite for a better understanding of the physiological traits controlling development.
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Song
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Zheng-Lin
authors.initials: ZL
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Sun
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China.
authors.forename: Dong-Xiu
authors.initials: DX
authors.lastname: Xue
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China tzhang@qdio.ac.cn haiyanwang@qdio.ac.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China tzhang@qdio.ac.cn haiyanwang@qdio.ac.cn.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D004064:Digestive System / Q000254:growth & development / Q000378:metabolism; D049851:Gastropoda / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D007107:Immune System / Q000254:growth & development / Q000378:metabolism; D007814:Larva / Q000235:genetics* / Q000254:growth & development; D058977:Molecular Sequence Annotation; D009420:Nervous System / Q000254:growth & development / Q000378:metabolism; D012758:Shellfish; D059467:Transcriptome
keywords: Rapana venosa; digital gene expression; gastropod; larva; transcriptome