title: Paracellular epithelial sodium transport maximizes energy efficiency in the kidney.
abstract: Efficient oxygen utilization in the kidney may be supported by paracellular epithelial transport, a form of passive diffusion that is driven by preexisting transepithelial electrochemical gradients. Claudins are tight-junction transmembrane proteins that act as paracellular ion channels in epithelial cells. In the proximal tubule (PT) of the kidney, claudin-2 mediates paracellular sodium reabsorption. Here, we used murine models to investigate the role of claudin-2 in maintaining energy efficiency in the kidney. We found that claudin-2-null mice conserve sodium to the same extent as WT mice, even during profound dietary sodium depletion, as a result of the upregulation of transcellular Na-K-2Cl transport activity in the thick ascending limb of Henle. We hypothesized that shifting sodium transport to transcellular pathways would lead to increased whole-kidney oxygen consumption. Indeed, compared with control animals, oxygen consumption in the kidneys of claudin-2-null mice was markedly increased, resulting in medullary hypoxia. Furthermore, tubular injury in kidneys subjected to bilateral renal ischemia-reperfusion injury was more severe in the absence of claudin-2. Our results indicate that paracellular transport in the PT is required for efficient utilization of oxygen in the service of sodium transport. We speculate that paracellular permeability may have evolved as a general strategy in epithelial tissues to maximize energy efficiency.
authors.forename: Lei
authors.initials: L
authors.lastname: Pei
authors.forename: Glenn
authors.initials: G
authors.lastname: Solis
authors.forename: Mien T X
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Nikhil
authors.initials: N
authors.lastname: Kamat
authors.forename: Lynn
authors.initials: L
authors.lastname: Magenheimer
authors.forename: Min
authors.initials: M
authors.lastname: Zhuo
authors.forename: Jiahua
authors.initials: J
authors.lastname: Li
authors.forename: Joshua
authors.initials: J
authors.lastname: Curry
authors.forename: Alicia A
authors.initials: AA
authors.lastname: McDonough
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Fields
authors.forename: William J
authors.initials: WJ
authors.lastname: Welch
authors.forename: Alan S L
authors.initials: AS
authors.lastname: Yu
mesh_terms: D000818:Animals; D062446:Claudin-2 / Q000378:metabolism*; D004032:Diet; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017136:Ion Transport; D007668:Kidney / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008138:Loop of Henle / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000652:urine; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D010539:Permeability; D015427:Reperfusion Injury / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D062446:Claudin-2; D008315:Malondialdehyde; D012964:Sodium; D008274:Magnesium; D010100:Oxygen