title: Discovery of the New Plant Growth-Regulating Compound LYXLF2 Based on Manipulating the Halogenase in Amycolatopsis orientalis.
abstract: Analysis of the Amycolatopsis orientalis HCCB10007 genome revealed new gene clusters involved in natural product biosynthesis that were not associated with the production of known compounds. Halogenases are a type of tailoring enzymes that are usually found within these secondary gene clusters. In this study, we identified an indole-type halometabolite 6-chrolo-1H-indole-3-carboxamide, named LYXLF2, by whole genome mining and metabolic profiling of a flavin-dependent halogenase mutant. LYXLF2 is a new plant growth-regulating compound that promotes root elongation. The results of this study demonstrated that the special gene knock-out/comparative metabolic profiling approach provides a powerful tool for the discovery of novel natural products by genome mining.
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, 800 Dongchuan Rd, Shanghai, 200240, China.|Shanghai Vocational College of Agriculture and Forest, Shanghai, 201600, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, 800 Dongchuan Rd, Shanghai, 200240, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.affiliation: Shanghai Laiyi Center for Biopharmaceuticals R&D, Shanghai, 201203, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ge
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, 800 Dongchuan Rd, Shanghai, 200240, China. zhulicyh@163.com.|Shanghai Laiyi Center for Biopharmaceuticals R&D, Shanghai, 201203, China. zhulicyh@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Pharmacy, Shanghai Jiaotong University, 800 Dongchuan Rd, Shanghai, 200240, China. qianxp@sjtu.edu.cn.
authors.forename: XiuPing
authors.initials: X
authors.lastname: Qian
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects* / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D007093:Imidazoles / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000737:chemistry* / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D010944:Plants
chemical_list: D001426:Bacterial Proteins; D007093:Imidazoles; D010937:Plant Growth Regulators; D006867:Hydrolases; C046137:haloalkane dehalogenase