title: Spatial and Temporal Profiling of Griseofulvin Production in Xylaria cubensis Using Mass Spectrometry Mapping.
abstract: A large portion of natural products research revolves around the discovery of new, bioactive chemical entities; however, studies to probe the biological purpose of such secondary metabolites for the host organism are often limited. Mass spectrometry mapping of secondary metabolite biosynthesis in situ can be used to probe a series of ecological questions about fungi that may be lost through traditional natural products chemistry extraction protocols. A griseofulvin-producing fungal culture of the Xylariaceae family, isolated as an endophyte of the tree Asimina triloba, was analyzed through a series of spatial and temporal mapping experiments. This fungus produced unique fungal characteristics, such as guttates and stroma, both of which were explored spatially. The distribution of griseofulvin on this culture in isolation was compared to its dispersal when grown in co-culture with a competing Penicillium species via a droplet-based surface sampling system. The fungistatic properties of griseofulvin were visualized, including the consequences for biosynthesis of polyhydroxyanthraquinones in a rival culture.
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro NC, USA.
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Sica
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro NC, USA.
authors.forename: Evan R
authors.initials: ER
authors.lastname: Rees
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro NC, USA.
authors.forename: Edem
authors.initials: E
authors.lastname: Tchegnon
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro NC, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Bardsley
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro NC, USA.
authors.forename: Huzefa A
authors.initials: HA
authors.lastname: Raja
authors.affiliation: Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro NC, USA.
authors.forename: Nicholas H
authors.initials: NH
authors.lastname: Oberlies
keywords: co-culture; endophytes; fungal secondary metabolites; griseofulvin; mass spectrometry mapping; polyhydroxyanthraquinones