title: Species diversity of the genus Osmundea (Ceramiales, Rhodophyta) in the Macaronesian region.
abstract: Species diversity within the genus Osmundea in the Macaronesian region was explored by conducting a comprehensive sampling in the Azores, the Canary, and the Madeira archipelagos. Toward identification, all specimens were first observed alive to verify the absence of corps en cerise, a diagnostic character for the genus and morphometric data were measured (thallus length and width, first-order branches length and width, branchlets length and width, cortical cell length and width in surface view, cortical cell length and width in transverse section). Specimens were sequenced for COI-5P (39 specimens) and three species delimitation methods (Generalized Mixed Yule Coalescent, Automatic Barcode Gap Discovery method, and Poisson Tree Processes) were used to assess the threshold between infra- and interspecific relationships. Subsequently, one or several sequences of plastid-encoded large subunit of RuBisCO (21 specimens) per delimited species were generated to assess the phylogenetic relationships among Macaronesian Osmundea. Moreover, for each delineated species, vegetative and reproductive anatomy was thoroughly documented and, when possible, specimens were either assigned to existing taxa or described as novel species. This integrative approach has provided data for (i) the presence of O. oederi, O. pinnatifida, and O. truncata in Macaronesia; (ii) the proposal of two novel species, O. prudhommevanreinei sp. nov. and O. silvae sp. nov.; and (iii) evidence of an additional species referred as "Osmundea sp.1," which is a sister taxon of O. hybrida.
authors.affiliation: Botánica, Ecología y Fisiología Vegetal, Universidad de La Laguna, 38071 La Laguna, Santa Cruz de Tenerife, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Machín-Sánchez
authors.affiliation: Institut de Systématique, Évolution, Biodiversité, ISYEB - UMR 7205 - CNRS, MNHN, UPMC, EPHE, Muséum national d'Histoire naturelle, Sorbonne Universités, 57 rue Cuvier, CP 39 75005, Paris, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Rousseau
authors.affiliation: Institut de Systématique, Évolution, Biodiversité, ISYEB - UMR 7205 - CNRS, MNHN, UPMC, EPHE, Muséum national d'Histoire naturelle, Sorbonne Universités, 57 rue Cuvier, CP 39 75005, Paris, France.
authors.forename: Line
authors.initials: L
authors.lastname: Le Gall
authors.affiliation: Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090, São Paulo, Brazil.
authors.forename: Valéria
authors.initials: V
authors.lastname: Cassano
authors.affiliation: CE3C - Centre for Ecology, Evolution and Environmental Changes/Azorean Biodiversity Group and Universidade dos Açores - Departamento de Biologia, 9501-801, Ponta Delgada, Açores, Portugal.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Neto
authors.affiliation: Hidrobiología, Universidad Autónoma Metropolitana-Iztapalapa, A.P. 55-535, México, D.F. 09340, México.
authors.forename: Abel
authors.initials: A
authors.lastname: Sentíes
authors.affiliation: Núcleo de Pesquisa em Ficologia, Instituto de Botânica, Av. Miguel Estéfano, 3687, 04301-902, São Paulo, Brazil.
authors.forename: Mutue
authors.initials: M
authors.lastname: T Fujii
authors.affiliation: Botánica, Ecología y Fisiología Vegetal, Universidad de La Laguna, 38071 La Laguna, Santa Cruz de Tenerife, Spain.
authors.forename: María Candelaria
authors.initials: MC
authors.lastname: Gil-Rodríguez
mesh_terms: D020418:Algal Proteins / Q000235:genetics; D001266:Atlantic Islands; D001394:Azores; D044822:Biodiversity; D058893:DNA Barcoding, Taxonomic; D010802:Phylogeny; D011174:Portugal; D000461:Rhodophyta / Q000145:classification* / Q000166:cytology / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D020418:Algal Proteins
keywords: Laurencia complex; Osmundea; Rhodomelaceae; species delimitation methods; systematics; taxonomy