title: [Cytokine gene polymorphisms in patients with age-related macular degeneration].
abstract: OBJECTIVE
  To establish possible association between age-related macular degeneration (AMD) and cytokine genotype polymorphisms; for that promoter regions of a number of cytokine genes were studied, namely, TNF-A863C, TNF-A308G, TNF-A238G, IL1β-C-31T, IL4-C590T, IL6-C174G, and IL10A-1082G.
  
  
  METHODS
  A total of 102 AMD and 100 non-AMD participants in the same age range were examined at the Novosibirsk branch of the Academician S.N. Fyodorov IRTC «Eye Microsurgery». In all cases restriction fragment length polymorphism analysis was performed.
  
  
  RESULTS
  The frequency of TNF-308 GA and IL10-1082 GG genotypes was higher in AMD patients than in the controls, while the TNF-308 АA minor genotype has proved protective against the disease. Moreover, AMD has been found to be positively related to 48 genotypic combinations and negatively - to 32 (with specificity as high as 98-99%). The odds ratio was also higher in combination analysis as compared to monogenotypes.
  
  
  CONCLUSIONS
  Complex genetic analysis of cytokines gives an idea of the balance of proinflammatory and anti-inflammatory cytokines in AMD and, therefore, plays an important role in studying the disease pathogenesis and exploring therapeutic options.
authors.affiliation: Research Institute of Clinical and Experimental Lymрhology, 2 Timakova St., Novosibirsk, Russian Federation, 630060.
authors.forename: A V
authors.initials: AV
authors.lastname: Shevchenko
authors.affiliation: Research Institute of Clinical and Experimental Lymрhology, 2 Timakova St., Novosibirsk, Russian Federation, 630060.
authors.forename: V F
authors.initials: VF
authors.lastname: Prokof'ev
authors.affiliation: Research Institute of Clinical and Experimental Lymрhology, 2 Timakova St., Novosibirsk, Russian Federation, 630060.
authors.forename: V I
authors.initials: VI
authors.lastname: Konenkov
authors.affiliation: Novosibirsk branch of the Academician S.N. Fyodorov IRTC 'Eye Microsurgery', Ministry of Health of the Russian Federation, 10 Kolkhidskaya St., Novosibirsk, Russian Federation, 630096.
authors.forename: V V
authors.initials: VV
authors.lastname: Chernykh
authors.affiliation: Novosibirsk branch of the Academician S.N. Fyodorov IRTC 'Eye Microsurgery', Ministry of Health of the Russian Federation, 10 Kolkhidskaya St., Novosibirsk, Russian Federation, 630096.
authors.forename: A V
authors.initials: AV
authors.lastname: Eremina
authors.affiliation: Novosibirsk branch of the Academician S.N. Fyodorov IRTC 'Eye Microsurgery', Ministry of Health of the Russian Federation, 10 Kolkhidskaya St., Novosibirsk, Russian Federation, 630096.
authors.forename: L V
authors.initials: LV
authors.lastname: Dudnikova
authors.affiliation: Novosibirsk branch of the Academician S.N. Fyodorov IRTC 'Eye Microsurgery', Ministry of Health of the Russian Federation, 10 Kolkhidskaya St., Novosibirsk, Russian Federation, 630096.
authors.forename: N Yu
authors.initials: NY
authors.lastname: Kashkina
authors.affiliation: Novosibirsk branch of the Academician S.N. Fyodorov IRTC 'Eye Microsurgery', Ministry of Health of the Russian Federation, 10 Kolkhidskaya St., Novosibirsk, Russian Federation, 630096.
authors.forename: A N
authors.initials: AN
authors.lastname: Trunov
mesh_terms: D000368:Aged; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008268:Macular Degeneration / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: C508609:IL10 protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10