title: Asymmetric division of clonal muscle stem cells coordinates muscle regeneration in vivo.
abstract: Skeletal muscle is an example of a tissue that deploys a self-renewing stem cell, the satellite cell, to effect regeneration. Recent in vitro studies have highlighted a role for asymmetric divisions in renewing rare "immortal" stem cells and generating a clonal population of differentiation-competent myoblasts. However, this model currently lacks in vivo validation. We define a zebrafish muscle stem cell population analogous to the mammalian satellite cell and image the entire process of muscle regeneration from injury to fiber replacement in vivo. This analysis reveals complex interactions between satellite cells and both injured and uninjured fibers and provides in vivo evidence for the asymmetric division of satellite cells driving both self-renewal and regeneration via a clonally restricted progenitor pool.
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: David B
authors.initials: DB
authors.lastname: Gurevich
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Phong Dang
authors.initials: PD
authors.lastname: Nguyen
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Ashley L
authors.initials: AL
authors.lastname: Siegel
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Ophelia V
authors.initials: OV
authors.lastname: Ehrlich
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Carmen
authors.initials: C
authors.lastname: Sonntag
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Jennifer M N
authors.initials: JM
authors.lastname: Phan
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Silke
authors.initials: S
authors.lastname: Berger
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Dhanushika
authors.initials: D
authors.lastname: Ratnayake
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Lucy
authors.initials: L
authors.lastname: Hersey
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Joachim
authors.initials: J
authors.lastname: Berger
authors.affiliation: School of Biological Sciences, Building 18, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Heather
authors.initials: H
authors.lastname: Verkade
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Hall
authors.affiliation: Australian Regenerative Medicine Institute, Level 1, 15 Innovation Walk, Monash University, Wellington Road, Clayton, Victoria 3800, Australia. European Molecular Biology Laboratory Australia Melbourne Node, Level 1, Building 75, Monash University, Wellington Road, Clayton, Victoria 3800, Australia. peter.currie@monash.edu.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Currie
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002455:Cell Division / Q000235:genetics / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods*; D002999:Clone Cells; D024510:Muscle Development / Q000235:genetics / Q000502:physiology; D018482:Muscle, Skeletal / Q000196:embryology / Q000293:injuries / Q000502:physiology*; D009154:Mutation; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics; D018008:Myogenin / Q000235:genetics; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000502:physiology*; D019076:Transgenes; D015027:Zebrafish
chemical_list: D051787:Myogenic Regulatory Factor 5; D018008:Myogenin