title: Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model.
abstract: Mesenchymal stem cells (MSCs) have a promising role as a therapeutic agent for neurodegenerative diseases such as Alzheimer's disease (AD). Prior studies suggested that intra-arterially administered MSCs are engrafted into the brain in stroke or traumatic brain injury (TBI) animal models. However, a controversial standpoint exists in terms of the integrity of the blood brain barrier (BBB) in transgenic AD mice. The primary goal of this study was to explore the feasibility of delivering human umbilical cord-blood derived mesenchymal stem cells (hUCB-MSCs) into the brains of non-transgenic WT (C3H/C57) and transgenic AD (APP/PS1) mice through the intra-arterial (IA) route. Through two experiments, mice were infused with hUCB-MSCs via the right internal carotid artery and were sacrificed at two different time points: 6 hours (experiment 1) or 5 minutes (experiment 2) after infusion. In both experiments, no cells were detected in the brain parenchyma while MSCs were detected in the cerebrovasculature in experiment 2. The results from this study highlight that intra-arterial delivery of MSCs is not the most favorable route to be implemented as a potential therapeutic approach for AD.
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Na Kyung
authors.initials: NK
authors.lastname: Lee
authors.affiliation: Laboratory Animal Research Center, Samsung Biomedical Research Institute, Gangnam-gu, Seoul, Republic of Korea.
authors.forename: Jehoon
authors.initials: J
authors.lastname: Yang
authors.affiliation: Samsung Biomedical Research Institute, Samsung Advanced Institute of Technology (SAIT), Samsung Electronics Co., Ltd., Seoul, 135-710, Republic of Korea.
authors.forename: Eun Hyuk
authors.initials: EH
authors.lastname: Chang
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Sang Eon
authors.initials: SE
authors.lastname: Park
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Jeongmin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., 463-400, Gyeonggi-do, Republic of Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., 463-400, Gyeonggi-do, Republic of Korea.
authors.forename: Wonil
authors.initials: W
authors.lastname: Oh
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Republic of Korea.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Chang
authors.affiliation: Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Duk L
authors.initials: DL
authors.lastname: Na
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology* / Q000628:therapy*; D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007269:Injections, Intra-Arterial; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D000072356:Parenchymal Tissue / Q000166:cytology*; D016896:Treatment Outcome