title: Genomic insights into a new acidophilic, copper-resistant Desulfosporosinus isolate from the oxidized tailings area of an abandoned gold mine.
abstract: Microbial sulfate reduction in acid mine drainage is still considered to be confined to anoxic conditions, although several reports have shown that sulfate-reducing bacteria occur under microaerophilic or aerobic conditions. We have measured sulfate reduction rates of up to 60 nmol S cm(-3) day(-1) in oxidized layers of gold mine tailings in Kuzbass (SW Siberia). A novel, acidophilic, copper-tolerant Desulfosporosinus sp. I2 was isolated from the same sample and its genome was sequenced. The genomic analysis and physiological data indicate the involvement of transporters and additional mechanisms to tolerate metals, such as sequestration by polyphosphates. Desulfosporinus sp. I2 encodes systems for a metabolically versatile life style. The genome possessed a complete Embden-Meyerhof pathway for glycolysis and gluconeogenesis. Complete oxidation of organic substrates could be enabled by the complete TCA cycle. Genomic analysis found all major components of the electron transfer chain necessary for energy generation via oxidative phosphorylation. Autotrophic CO2 fixation could be performed through the Wood-Ljungdahl pathway. Multiple oxygen detoxification systems were identified in the genome. Taking into account the metabolic activity and genomic analysis, the traits of the novel isolate broaden our understanding of active sulfate reduction and associated metabolism beyond strictly anaerobic niches.
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Prosp. 60-let Oktiabria, Building 7-1, 119071 Moscow, Russia.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Mardanov
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Lenina prospect 36, 634050 Tomsk, Russia.
authors.forename: Inna A
authors.initials: IA
authors.lastname: Panova
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Prosp. 60-let Oktiabria, Building 7-1, 119071 Moscow, Russia.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Beletsky
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Lenina prospect 36, 634050 Tomsk, Russia.
authors.forename: Marat R
authors.initials: MR
authors.lastname: Avakyan
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Prosp. 60-let Oktiabria, Building 7-1, 119071 Moscow, Russia.
authors.forename: Vitaly V
authors.initials: VV
authors.lastname: Kadnikov
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Lenina prospect 36, 634050 Tomsk, Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Antsiferov
authors.affiliation: School of Engineering, Systems Power and Energy, Glasgow University, James Watt Building (South), Glasgow G12 8QQ and Holymoor Consultancy Ltd, 360 Ashgate Road, Chesterfield, Derbyshire S40 4BW, UK.
authors.forename: David
authors.initials: D
authors.lastname: Banks
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Lenina prospect 36, 634050 Tomsk, Russia.
authors.forename: Yulia A
authors.initials: YA
authors.lastname: Frank
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Pr. 60-letiya Oktyabrya 7/2, 119071 Moscow, Russia.
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Pimenov
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Prosp. 60-let Oktiabria, Building 7-1, 119071 Moscow, Russia.
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Ravin
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Tomsk State University, Lenina prospect 36, 634050 Tomsk, Russia olga.karnachuk@green.tsu.ru.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Karnachuk
mesh_terms: D000222:Adaptation, Physiological; D003300:Copper / Q000378:metabolism; D023281:Genomics; D006046:Gold; D008906:Mining; D010084:Oxidation-Reduction; D010459:Peptococcaceae / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D012800:Siberia; D013431:Sulfates / Q000378:metabolism
chemical_list: D013431:Sulfates; D006046:Gold; D003300:Copper
keywords: Desulfosporosinus; acidophilic sulfate-reducing bacteria; metal resistance; polyphosphates