title: Development of microsatellite markers for Sargentodoxa cuneata (Lardizabalaceae) using next-generation sequencing technology.
abstract: OBJECTIVE
  Microsatellite loci were developed for a woody deciduous liana, Sargentodoxa cuneata (Lardizabalaceae), to help infer the evolutionary histories of ancient monotypic genera in subtropical China.
  
  
  RESULTS
  Using next-generation sequencing (Illumina MiSeq) technology, 21 polymorphic primer sets were identified in three wild populations. The number of alleles per locus ranged from one to seven. The expected and observed heterozygosities varied from 0 to 0.788 and 0 to 0.917, respectively. Transferability analyses were performed in Stauntonia chinensis, Akebia trifoliata, and A. quinata. Eighteen (85.7%), 18 (85.7%), and 17 (81.0%) markers were successfully amplified, respectively.
  
  
  CONCLUSIONS
  The newly developed markers will facilitate further studies on genetic diversity and phylogeographic patterns throughout the distributional range of S. cuneata. This set of microsatellite primers represents the second report on molecular markers in Lardizabalaceae.
authors.affiliation: College of Forestry, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China; Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Zhi-Xia
authors.initials: ZX
authors.lastname: Sun
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China; Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China.
authors.forename: Lin-Jiang
authors.initials: LJ
authors.lastname: Ye
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Forestry, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China; Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Wan
authors.initials: W
authors.lastname: Hu
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Deng-Mei
authors.initials: DM
authors.lastname: Fan
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
keywords: Illumina MiSeq; Lardizabalaceae; Sargentodoxa cuneata; genetic diversity; microsatellite markers