title: Trabecular and Cortical Bone of Growing C3H Mice Is Highly Responsive to the Removal of Weightbearing.
abstract: Genetic make-up strongly influences the skeleton's susceptibility to the loss of weight bearing with some inbred mouse strains experiencing great amounts of bone loss while others lose bone at much smaller rates. At young adulthood, female inbred C3H/HeJ (C3H) mice are largely resistant to catabolic pressure induced by unloading. Here, we tested whether the depressed responsivity to unloading is inherent to the C3H genetic make-up or whether a younger age facilitates a robust skeletal response to unloading. Nine-week-old, skeletally immature, female C3H mice were subjected to 3wk of hindlimb unloading (HLU, n = 12) or served as normal baseline controls (BC, n = 10) or age-matched controls (AC, n = 12). In all mice, cortical and trabecular architecture of the femur, as well as levels of bone formation and resorption, were assessed with μCT, histomorphometry, and histology. Changes in bone marrow progenitor cell populations were determined with flow cytometry. Following 21d of unloading, HLU mice had 52% less trabecular bone in the distal femur than normal age-matched controls. Reflecting a loss of trabecular tissue compared to baseline controls, trabecular bone formation rates (BFR/BS) in HLU mice were 40% lower than in age-matched controls. Surfaces undergoing osteoclastic resorption were not significantly different between groups. In the mid-diaphysis, HLU inhibited cortical bone growth leading to 14% less bone area compared to age-matched controls. Compared to AC, BFR/BS of HLU mice were 53% lower at the endo-cortical surface and 49% lower at the periosteal surface of the mid-diaphysis. The enriched osteoprogenitor cell population (OPC) comprised 2% of the bone marrow stem cells in HLU mice, significantly different from 3% OPC in the AC group. These data show that bone tissue in actively growing C3H mice is lost rapidly, or fails to grow, during the removal of functional weight bearing-in contrast to the insignificant response previously demonstrated in female young adult C3H mice. Thus, the attributed low sensitivity of the C3H mouse strain to the loss of mechanical signals is not apparent at a young age and this trait therefore does not reflect a genetic regulation throughout the life span of this strain. These results highlight the significance of age in modulating the contribution of genetics in orchestrating bone's response to unloading and that the skeletal unresponsiveness of young adult C3H mice to the loss of weight bearing is not genetically hard-wired.
authors.affiliation: Department of Orthopedics, Tianjin Hospital, Tianjin, 300211, China.|Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, United States of America.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, United States of America.
authors.forename: Jeyantt Srinivas
authors.initials: JS
authors.lastname: Sankaran
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, United States of America.
authors.forename: Stefan
authors.initials: S
authors.lastname: Judex
mesh_terms: D000818:Animals; D001846:Bone Development; D000071556:Cancellous Bone / Q000000981:diagnostic imaging* / Q000503:physiopathology; D000071538:Cortical Bone / Q000000981:diagnostic imaging* / Q000503:physiopathology; D005260:Female; D019417:Hindlimb Suspension / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008809:Mice, Inbred C3H; D016474:Weight-Bearing; D055114:X-Ray Microtomography / Q000379:methods