title: Apolipoproteins L control cell death triggered by TLR3/TRIF signaling in dendritic cells.
abstract: Apolipoproteins L (ApoLs) are Bcl-2-like proteins expressed under inflammatory conditions in myeloid and endothelial cells. We found that Toll-like receptor (TLR) stimuli, particularly the viral mimetic polyinosinic:polycytidylic acid (poly(I:C)), specifically induce ApoLs7/11 subfamilies in murine CD8α(+)  dendritic cells (DCs). This induction requires the TLR3/TRIF (where TRIF is TIR domain containing adapter-inducing interferon β) signaling pathway and is dependent on IFN-β in all ApoLs subfamilies except for ApoL7c. Poly(I:C) treatment of DCs is also associated with induction of both cell death and autophagy. ApoLs expression is related to promotion of DC death by poly(I:C), as ApoLs7/11 knockdown increases DC survival and ApoLs7 are associated with the anti-apoptotic protein Bcl-xL (where Bcl-xL is B-cell lymphoma extra large). Similarly, in human monocyte-derived DCs poly(I:C) induces both cell death and the expression of ApoLs, principally ApoL3. Finally, the BH3-like peptide of ApoLs appears to be involved in the DC death-promoting activity. We would like to propose that ApoLs are involved in cell death linked to activation of DCs by viral stimuli.
authors.affiliation: Laboratoire de Parasitologie Moléculaire, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Sophie
authors.initials: S
authors.lastname: Uzureau
authors.affiliation: Laboratoire d'Immunobiologie, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Coquerelle
authors.affiliation: Laboratoire de Parasitologie Moléculaire, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Corentin
authors.initials: C
authors.lastname: Vermeiren
authors.affiliation: Laboratoire de Parasitologie Moléculaire, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.|Laboratoire de Médecine Expérimentale, Hôpital Vésale, Université Libre de Bruxelles, Montigny-le-Tilleul, Belgium.
authors.forename: Pierrick
authors.initials: P
authors.lastname: Uzureau
authors.affiliation: Laboratoire d'Immunobiologie, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Annette
authors.initials: A
authors.lastname: Van Acker
authors.affiliation: de Duve Institute, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Pilotte
authors.affiliation: Laboratoire de Parasitologie Moléculaire, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Daniel
authors.initials: D
authors.lastname: Monteyne
authors.affiliation: Laboratoire d'Immunobiologie, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Valérie
authors.initials: V
authors.lastname: Acolty
authors.affiliation: Laboratoire de Parasitologie Moléculaire, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Benoit
authors.initials: B
authors.lastname: Vanhollebeke
authors.affiliation: de Duve Institute, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Benoît
authors.initials: B
authors.lastname: Van den Eynde
authors.affiliation: Laboratoire de Parasitologie Moléculaire, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Pérez-Morga
authors.affiliation: Laboratoire d'Immunobiologie, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Muriel
authors.initials: M
authors.lastname: Moser
authors.affiliation: Laboratoire de Parasitologie Moléculaire, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Etienne
authors.initials: E
authors.lastname: Pays
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001053:Apolipoproteins / Q000276:immunology*; D017209:Apoptosis; D016827:CD8 Antigens / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011070:Poly I-C / Q000494:pharmacology; D020033:Protein Isoforms / Q000276:immunology; D015398:Signal Transduction; D051196:Toll-Like Receptor 3 / Q000378:metabolism*; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D001053:Apolipoproteins; D016827:CD8 Antigens; D016899:Interferon-beta; D011070:Poly I-C; D020033:Protein Isoforms; D051196:Toll-Like Receptor 3; D051020:bcl-X Protein; C494812:Bcl2l1 protein, mouse; C476286:Ticam1 protein, mouse
keywords: ApoL1; Apolipoproteins L; Bcl-xL; Cell death; Dendritic cells ⋅ Poly(I :C); TLR3; TRIF