title: Binding of Daptomycin to Anionic Lipid Vesicles Is Reduced in the Presence of Lysyl-Phosphatidylglycerol.
abstract: The cytoplasmic membrane of Staphylococcus aureus contains ∼20 mol% of the net cationic lipid lysyl-phosphatidylglycerol (LPG). Elevated fractions of LPG are associated with increased resistance to cationic antibiotics, including the lipopeptide daptomycin (DAP). Although the surface charge of the bacterial cytoplasmic membrane is altered by LPG, surface binding of DAP was found to be only moderately affected in anionic vesicles containing 20 mol% LPG. These results suggest that charge repulsion cannot fully explain LPG-mediated resistance to cationic peptides.
authors.affiliation: University of North Carolina Wilmington, Department of Chemistry and Biochemistry, Wilmington, North Carolina, USA.
authors.forename: Tala O
authors.initials: TO
authors.lastname: Khatib
authors.affiliation: University of North Carolina Wilmington, Department of Chemistry and Biochemistry, Wilmington, North Carolina, USA.
authors.forename: Heather
authors.initials: H
authors.lastname: Stevenson
authors.affiliation: Divisions of Molecular Medicine, Los Angeles Biomedical Research Institute at Harbor-University of California at Los Angeles Medical Center, Torrance, California, USA Division of Adult Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-University of California at Los Angeles Medical Center, California, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Yeaman
authors.affiliation: Divisions of Molecular Medicine, Los Angeles Biomedical Research Institute at Harbor-University of California at Los Angeles Medical Center, Torrance, California, USA.
authors.forename: Arnold S
authors.initials: AS
authors.lastname: Bayer
authors.affiliation: University of North Carolina Wilmington, Department of Chemistry and Biochemistry, Wilmington, North Carolina, USA almeidaa@uncw.edu.
authors.forename: Antje
authors.initials: A
authors.lastname: Pokorny
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D017576:Daptomycin / Q000737:chemistry* / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010715:Phosphatidylglycerols / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D008055:Lipids; D010715:Phosphatidylglycerols; C002285:lysylphosphatidylglycerol; D008239:Lysine; D017576:Daptomycin