title: Graphene nanosheet mediated MALDI-MS (GN-MALDI-MS) for rapid, in situ detection of intact incipient biofilm on material surfaces.
abstract: Detection is the first step to efficient treatment, therefore early detection of biofilm gains paramount importance for the initiation of mitigation protocols. A systematic study was conducted to detect the biofilm formation (1h to 2month period) on aluminium, titanium surfaces and their corresponding oxide film surfaces. The limit of detection (LOD) in case of traditional MALDI-MS was limited to a 6h old biofilm. Whereas, in case of the Graphene nanosheet mediated MALDI-MS (GN-MALDI-MS) approach, early detection of the biofilm was demonstrated to be 1h on titanium surfaces and 3h for Al surfaces.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan.
authors.forename: Bo Sgum
authors.initials: BS
authors.lastname: Wu
authors.affiliation: Department of Molecular Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Judy
authors.initials: J
authors.lastname: Gopal
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan.
authors.forename: Pei-Yang
authors.initials: PY
authors.lastname: Hua
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institute of Medical Science and Technology, National Sun Yat-Sen University, 80424, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung 80424, Taiwan; Center for Nanosciences and Nanotechnology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan. Electronic address: hwu@faculty.nsysu.edu.tw.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D000535:Aluminum / Q000737:chemistry; D018441:Biofilms / Q000254:growth & development; D006108:Graphite / Q000737:chemistry*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus / Q000502:physiology; D014025:Titanium / Q000737:chemistry; D044149:Vibrio alginolyticus / Q000502:physiology
chemical_list: D006108:Graphite; D000535:Aluminum; D014025:Titanium
keywords: Aluminium; Biofilm; Detection; Graphene; Heat treatment; MALDI-MS; Titanium