title: Impact of thermal annealing on wettability and antifouling characteristics of alginate poly-l-lysine polyelectrolyte multilayer films.
abstract: Polyelectrolyte multilayers (PEMs) of poly-l-lysine (PLL) and alginic acid sodium salt (Alg) are fabricated applying the layer by layer technique and annealed at a constant temperature; 37, 50 and 80°C, for 72h. Atomic force microscopy reveals changes in the topography of the PEM, which is changing from a fibrillar to a smooth surface. Advancing contact angle in water varies from 36° before annealing to 93°, 77° and 95° after annealing at 37, 50 and 80°C, respectively. Surface energy changes after annealing were calculated from contact angle measurements performed with organic solvents. Quartz crystal microbalance with dissipation, contact angle and fluorescence spectroscopy measurements show a significant decrease in the adsorption of the bovine serum albumin protein to the PEMs after annealing. Changes in the physical properties of the PEMs are interpreted as a result of the reorganization of the polyelectrolytes in the PEMs from a layered structure into complexes where the interaction of polycations and polyanions is enhanced. This work proposes a simple method to endow bio-PEMs with antifouling characteristics and tune their wettability.
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Miramón 182C, 20009 San Sebastián, Guipúzcoa, Spain.
authors.forename: Eleftheria
authors.initials: E
authors.lastname: Diamanti
authors.affiliation: Instituto de Investigaciones Fisicoquimicas Teóricas y Aplicadas (INIFTA), (UNLP, CONICET), Sucursal 4, Casilla de Correo 16, 1900 La Plata, Argentina.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Muzzio
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Miramón 182C, 20009 San Sebastián, Guipúzcoa, Spain.
authors.forename: Danijela
authors.initials: D
authors.lastname: Gregurec
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Miramón 182C, 20009 San Sebastián, Guipúzcoa, Spain.
authors.forename: Joseba
authors.initials: J
authors.lastname: Irigoyen
authors.affiliation: Instituto de Investigaciones Fisicoquimicas Teóricas y Aplicadas (INIFTA), (UNLP, CONICET), Sucursal 4, Casilla de Correo 16, 1900 La Plata, Argentina.
authors.forename: Miguel
authors.initials: M
authors.lastname: Pasquale
authors.affiliation: Instituto de Investigaciones Fisicoquimicas Teóricas y Aplicadas (INIFTA), (UNLP, CONICET), Sucursal 4, Casilla de Correo 16, 1900 La Plata, Argentina.
authors.forename: Omar
authors.initials: O
authors.lastname: Azzaroni
authors.affiliation: Experimental Physics, Saarland University, 66123 Sarbruecken, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Brinkmann
authors.affiliation: Soft Matter Nanotechnology Group, CIC biomaGUNE, Paseo Miramón 182C, 20009 San Sebastián, Guipúzcoa, Spain. Electronic address: smoya@cicbiomagune.es.
authors.forename: Sergio Enrique
authors.initials: SE
authors.lastname: Moya
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D057219:Biofouling; D002417:Cattle; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D000071228:Polyelectrolytes / Q000494:pharmacology*; D011107:Polylysine / Q000494:pharmacology*; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry; D017155:Wettability
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D000071228:Polyelectrolytes; D014867:Water; D011107:Polylysine; D012710:Serum Albumin, Bovine; D020723:Glucuronic Acid
keywords: Antifouling; Biopolymers; Polyelectrolyte multilayers; Thermal annealing; Wettability