title: A comparative study of 5-Fluorouracil release from chitosan/silver and chitosan/silver/MWCNT nanocomposites and their cytotoxicity towards MCF-7.
abstract: 5-Fluorouracil encapsulated chitosan/silver and chitosan/silver/multiwalled carbon nanotubes were synthesized to comparatively study the release profile and cytotoxicity of the systems towards MCF-7 cell line. The triclinic structure of 5-Fluorouracil, face centered cubic structure of silver and the semi-crystalline nature of chitosan were elucidated using the XRD pattern. The XRD pattern of Chitosan/silver/multiwalled carbon nanotube consisted of (002) reflection of graphitic carbon from carbon nanotube. The evident splitting of NH2 and NH3(+) and a variation in the intensity of OH peaks in the FTIR pattern were indicative of the binding of moieties like silver, carbon nanotube and 5-Fluorouracil to chitosan. The encapsulation of 5-Fluorouracil was evident from elemental mapping and from the presence of reflections corresponding to 5-Fluorouracil in the SAED pattern. The release profile showed a prolonged release for 5-Fluorouracil encapsulated Chitosan/silver/multiwalled carbon nanotube and a better cytotoxicity with a IC50 of 50μg/ml was observed for the same.
authors.affiliation: Material Science Centre, Department of Nuclear Physics, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Nivethaa
authors.initials: N
authors.lastname: E A K
authors.affiliation: Material Science Centre, Department of Nuclear Physics, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Dhanavel
authors.initials: D
authors.lastname: S
authors.affiliation: Material Science Centre, Department of Nuclear Physics, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Rebekah
authors.initials: R
authors.lastname: A
authors.affiliation: Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Narayanan
authors.initials: N
authors.lastname: V
authors.affiliation: Material Science Centre, Department of Nuclear Physics, University of Madras, Guindy Campus, Chennai 600 025, India. Electronic address: stephen_arum@hotmail.com.
authors.forename: Stephen
authors.initials: S
authors.lastname: A
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D005472:Fluorouracil / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D037742:Nanotubes, Carbon; D012834:Silver; D048271:Chitosan; D005472:Fluorouracil
keywords: 5-Fluorouracil; Cytotoxicity; MCF-7; Nanocomposite; Release profile