title: Increased ZAP70 Is Involved in Dry Skin Pruritus in Aged Mice.
abstract: Dry skin pruritus is common in the elderly. Recent reports show that T-cell signal path is involved in dry skin pruritus. Zeta-chain-associated protein kinase 70 (ZAP70), as a T-cell receptor, may induce interleukin 2 (IL-2) secretion and promote nerve growth factor (NGF) secretion in skin. This study aimed to detect the alteration of ZAP70 in a mice model with dry skin pruritus. The C57BL mice with 5 months and 22 months were used as experimental animal. Following a 5-day period of treatment of back with a mixture of acetone-diethyl-ether-water (AEW), mice exhibited a significant increase in spontaneous scratching behavior directed to the treated back compared to control animals in which back was similarly treated with water only (W). After AEW process, spontaneous scratching in 22-month AEW mice was increased compared to 5-month AEW mice. Western blot and real-time quantitative PCR data analysis showed that ZAP70 expression was significantly increased in 22-month AEW mice compared with 5-month AEW mice. ELISA data showed that secretions of IL-2 and NGF in 22-month AEW mice were higher than 5-month AEW mice. Our results indicate that increased ZAP70 is involved in dry skin in elderly pruritus. Increased secretion of IL-2 and NGF may induce dry skin itch.
authors.affiliation: Institute of Neuroscience, Department of Human Anatomy, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Min
authors.initials: M
authors.lastname: Gu
authors.affiliation: Institute of Neuroscience, Department of Human Anatomy, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tian
authors.affiliation: Institute of Neuroscience, Department of Human Anatomy, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Ru
authors.affiliation: Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lü
authors.affiliation: Institute of Neuroscience, Department of Human Anatomy, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Yu
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D007057:Ichthyosis / Q000378:metabolism / Q000473:pathology; D007376:Interleukin-2 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D020932:Nerve Growth Factor / Q000378:metabolism*; D011537:Pruritus / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D014018:Tissue Distribution; D015854:Up-Regulation; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000378:metabolism*
chemical_list: D007376:Interleukin-2; D020932:Nerve Growth Factor; D051746:ZAP-70 Protein-Tyrosine Kinase; C497262:Zap70 protein, mouse