title: The Effect of Autologous Platelet Lysate Eye Drops: An In Vivo Confocal Microscopy Study.
abstract: Purpose. To determine the effectiveness of autologous platelet lysate (APL) eye drops in patients with primary Sjögren syndrome (SS) dry eye, refractory to standard therapy, in comparison with patients treated with artificial tears. We focused on the effect of APL on cornea morphology with the in vivo confocal microscopy (IVCM). Methods. Patients were assigned to two groups: group A used autologous platelet lysate QID, and group B used preservative-free artificial tears QID, for 90 days. Ophthalmological assessments included ocular surface disease index (OSDI), best corrected visual acuity (BCVA), Schirmer test, fluorescein score, and breakup time (BUT). A subgroup of patients in group A underwent IVCM: corneal basal epithelium, subbasal nerves, Langerhans cells, anterior stroma activated keratocytes, and reflectivity were evaluated. Results. 60 eyes of 30 patients were enrolled; in group A (n = 20 patients) mean OSDI, fluorescein score, and BUT showed significant improvement compared with group B (n = 10 patients). The IVCM showed a significant increase in basal epithelium cells density and subbasal nerve plexus density and number and a decrease in Langerhans cells density (p < 0.05). Conclusion. APL was found effective in the treatment of SS dry eye. IVCM seems to be a useful tool to visualize cornea morphologic modifications.
authors.affiliation: Department of Clinical Sciences, Ophthalmology Institute, University of Turin, 10122 Turin, Italy.
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Fea
authors.affiliation: Department of Clinical Sciences, Ophthalmology Institute, University of Turin, 10122 Turin, Italy.
authors.forename: Vittoria
authors.identifier: 0000-0002-3870-9838
authors.initials: V
authors.lastname: Aragno
authors.affiliation: Department of Clinical Sciences, Ophthalmology Institute, University of Turin, 10122 Turin, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Testa
authors.affiliation: Department of Clinical Sciences, Ophthalmology Institute, University of Turin, 10122 Turin, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Machetta
authors.affiliation: Rheumatology Department, AOU Città della Salute e della Scienza di Torino, 10134 Turin, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Parisi
authors.affiliation: Blood Bank, AOU Città della Salute e della Scienza di Torino, 10134 Turin, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: D'Antico
authors.affiliation: Department of Clinical Sciences, Ophthalmology Institute, University of Turin, 10122 Turin, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Spinetta
authors.affiliation: Rheumatology Department, AOU Città della Salute e della Scienza di Torino, 10134 Turin, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Fusaro
authors.affiliation: Department of Clinical Sciences, Ophthalmology Institute, University of Turin, 10122 Turin, Italy.
authors.forename: Federico M
authors.initials: FM
authors.lastname: Grignolo
mesh_terms: D000368:Aged; D001324:Autoantigens; D001792:Blood Platelets / Q000276:immunology / Q000502:physiology*; D016022:Case-Control Studies; D002458:Cell Fractionation; D003315:Cornea / Q000000981:diagnostic imaging / Q000473:pathology; D015352:Dry Eye Syndromes / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D005260:Female; D006801:Humans; D065346:Lubricant Eye Drops / Q000627:therapeutic use*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D009883:Ophthalmic Solutions; D011446:Prospective Studies; D012859:Sjogren's Syndrome / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*
chemical_list: D001324:Autoantigens; D065346:Lubricant Eye Drops; D009883:Ophthalmic Solutions