title: Nipah Virus C Protein Recruits Tsg101 to Promote the Efficient Release of Virus in an ESCRT-Dependent Pathway.
abstract: The budding of Nipah virus, a deadly member of the Henipavirus genus within the Paramyxoviridae, has been thought to be independent of the host ESCRT pathway, which is critical for the budding of many enveloped viruses. This conclusion was based on the budding properties of the virus matrix protein in the absence of other virus components. Here, we find that the virus C protein, which was previously investigated for its role in antagonism of innate immunity, recruits the ESCRT pathway to promote efficient virus release. Inhibition of ESCRT or depletion of the ESCRT factor Tsg101 abrogates the C enhancement of matrix budding and impairs live Nipah virus release. Further, despite the low sequence homology of the C proteins of known henipaviruses, they all enhance the budding of their cognate matrix proteins, suggesting a conserved and previously unknown function for the henipavirus C proteins.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at the University of California-Los Angeles, Los Angeles, California, United States of America.|Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
authors.forename: Arnold
authors.identifier: 0000-0001-8916-2230
authors.initials: A
authors.lastname: Park
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Yun
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
authors.forename: Frederic
authors.initials: F
authors.lastname: Vigant
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at the University of California-Los Angeles, Los Angeles, California, United States of America.
authors.forename: Olivier
authors.initials: O
authors.lastname: Pernet
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
authors.forename: Sohui T
authors.initials: ST
authors.lastname: Won
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America.
authors.forename: Brian E
authors.initials: BE
authors.lastname: Dawes
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at the University of California-Los Angeles, Los Angeles, California, United States of America.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bartkowski
authors.affiliation: Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America.|Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, United States of America.|Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, United States of America.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Freiberg
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at the University of California-Los Angeles, Los Angeles, California, United States of America.|Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
authors.forename: Benhur
authors.initials: B
authors.lastname: Lee
mesh_terms: D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D057809:HEK293 Cells; D045464:Henipavirus Infections / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D045405:Nipah Virus / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D057074:Virus Release / Q000502:physiology*; D000099177:Tsg101 Protein
chemical_list: D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D010750:Phosphoproteins; D014157:Transcription Factors; D014764:Viral Proteins; D000099177:Tsg101 Protein; C543302:C protein, Nipah virus