title: Clamping of RNA with PNA enables targeting of microRNA.
abstract: To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, 141 83 Huddinge, Stockholm, Sweden. Roger.Stromberg@ki.se.
authors.forename: Alice
authors.initials: A
authors.lastname: Ghidini
authors.forename: Helen
authors.initials: H
authors.lastname: Bergquist
authors.forename: Merita
authors.initials: M
authors.lastname: Murtola
authors.forename: Tanel
authors.initials: T
authors.lastname: Punga
authors.forename: Rula
authors.initials: R
authors.lastname: Zain
authors.forename: Roger
authors.initials: R
authors.lastname: Strömberg
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D020135:Peptide Nucleic Acids; D012313:RNA