title: Mode of action of leucocin K7 produced by Leuconostoc mesenteroides K7 against Listeria monocytogenes and its potential in milk preservation.
abstract: OBJECTIVE
  To investigate the mode of action of leucocin K7 against Listeria monocytogenes and to assess its inhibitory effect on Lis. monocytogenes in refrigerated milk.
  
  
  RESULTS
  A bacteriocin-producing strain, Leuconostoc mesenteroides K7, was isolated from a fermented pickle. The bacteriocin, leucocin K7, exhibited antagonistic activity against Lis. monocytogenes with an MIC of 28 µg/ml. It was sensitive to proteaseS and displayed good thermal stability and broad active pH range. Leucocin K7 had no effect on the efflux of ATP from Lis. monocytogenes but triggered the efflux of K(+) and the intracellular hydrolysis of ATP. It also dissipated the transmembrane electrical potential completely and transmembrane pH gradient partially. It 80 AU/ml inhibited the growth of Lis. monocytogenes by 2.3-3.9 log units in milk; when combined with glycine (5 mg/ml), it completely eliminated viable Lis. monocytogenes over 7 days
  
  
  CONCLUSIONS
  Leucocin K7 shows different mode of action from nisin and may have potential application in milk preservation.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu, Wuxi, 214122, China. shifeng@jiangnan.edu.cn.|Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. shifeng@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China. shifeng@jiangnan.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu, Wuxi, 214122, China.|Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu, Wuxi, 214122, China.|Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000378:metabolism* / Q000494:pharmacology; D005519:Food Preservation / Q000379:methods*; D000070007:Leuconostoc mesenteroides / Q000235:genetics / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000378:metabolism; D008892:Milk
chemical_list: D001430:Bacteriocins
keywords: Bacteriocin; Leucocin K7; Leuconostoc mesenteroides; Listeria monocytogenes; Milk preservation; Mode of action