title: Preparation of complementary glycosylated hyperbranched polymer/poly(ethylene glycol) brushes and their selective interactions with hepatocytes.
abstract: Selective cell adhesion and migration, which mimics the natural biological events in vivo, is very important for the right repair of damaged tissues. In this study, glycosylated hyperbranched polymers (LA-HPMA) were synthesized, and were grafted on glass slide through dopamine deposition with different densities adjusted by co-grafting of poly(ethylene glycol) (PEG). The LA-HPMA and PEG molecular brushes were characterized by X-ray photoelectron spectroscopy (XPS), quartz crystal microbalance with dissipation (QCM-d) and ellipsometry. The adhesion of human hepatoma (HepG2) cells was promoted on the surface of a higher LA-HPMA density, and the migration rate was accelerated from 6.4μm/h on PEG surface to 12.7μm/h on 75% LA-HPMA surface. By contrast, the density and spreading area of mouse embryonic fibroblast (NIH3T3) cells were not significantly influenced by the LA-HPMA density, and the migration rate did not change significantly on all types of surfaces either. Therefore, the specific interactions of carbohydrate-protein can be used to modulate cell behaviors in vitro, for example the selective adhesion and migration of HepG2 cells.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Su
authors.initials: S
authors.lastname: Liang
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yu
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: cygao@mail.hz.zj.cn.
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000818:Animals; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D018920:Coculture Techniques; D004187:Disaccharides / Q000737:chemistry; D006031:Glycosylation / Q000187:drug effects; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D011092:Polyethylene Glycols / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D058525:Quartz Crystal Microbalance Techniques; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D004187:Disaccharides; D011092:Polyethylene Glycols; C005608:lactobionic acid
keywords: Carbohydrate; Cell adhesion; Cell migration; Hyperbranched polymers; Selectivity