title: Differential Cytokine Regulatory Effect of Three Lactobacillus Strains Isolated from Fermented Foods.
abstract: Lactic acid bacteria (LAB) isolated from fermented foods have potential as a treatment for immune-related disorders and the use of LAB has been increasing worldwide. In this study, the differential cytokine regulatory effect was examined with three isolates of lactobacilli strains; namely, Lactobacillus plantarum K55-5 isolated from dairy product, and L. sakei K101 and L. plantarum K8 previously isolated from kimchi (a Korean traditional fermented vegetable). Production of cytokines such as IL-10, IL-12, IFN-γ, and TNF-α was significantly increased in L. sakei K101- and L. plantarum K55-5-treated splenocytes as compared with controls. The oral administration of L. sakei K101 and L. plantarum K55-5 increased cytokine production in the immunosuppressed mouse splenocytes and blood. NK cell cytotoxic activity was also increased in L. sakei K101- and L. plantarum K55-5-fed mice. On the other hand, L. plantarum K8 did not affect cytokine induction in all the experiments performed in this study. The cytokine-inducing effect of L. plantarum K55-5 was significantly increased by lysates of heat-killed bacteria as compared with live, heat-killed, or supernatant of cell lysates. TNF-α production by lipoteichoic acids (LTAs) isolated from the three isolates of lactobacilli was compared, and it was found that K55-5 LTA had a highest cytokine-inducing ability, which was mediated by TLR2-mediated NF-κB and ERK activation. Taken together, our study suggests that L. plantarum K55-5 and L. sakei K101 can be used for the treatment of immunosuppressed disorders.
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Yoon-Doo
authors.initials: YD
authors.lastname: Lee
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.|Skin Biotechnology Center, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Hong
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Jeon
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Bong Jun
authors.initials: BJ
authors.lastname: Jung
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.|Skin Biotechnology Center, Kyung Hee University, Yongin 17104, Republic of Korea.|RNA Inc., College of Life Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
authors.affiliation: Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.|Skin Biotechnology Center, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Hangeun
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D043302:Cultured Milk Products / Q000382:microbiology*; D016207:Cytokines / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D005260:Female; D005516:Food Microbiology; D007107:Immune System / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D007778:Lactobacillus / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D056910:Republic of Korea; D015398:Signal Transduction
chemical_list: D016207:Cytokines
keywords: Lactobacillus plantarum; Lactobacillus sakei; cytokine; immunosuppression; inflammation; lipoteichoic acid