title: Pretreatment of bamboo by ultra-high pressure explosion with a high-pressure homogenizer for enzymatic hydrolysis and ethanol fermentation.
abstract: Bamboo shoots, 2- and 5-year-old bamboo were treated by using a homogenizer in a constant suspended state, a process termed as ultra-high pressure explosion (UHPE). The bamboo powder was heated in 2% NaOH solution at 121°C, and then 100MPa UHPE-treated through a homogenizer. The results verified that UHPE changed the suspension solution of powder into a stick fluid. The contents of lignin were decreased significantly. The bamboo shoots and 2-year-old bamboo were completely hydrolyzed to glucose within 48h by enzymes loading of 15 FPU of cellulase and 30IU of β-glucosidase per gram glucan. Fermentation of enzymatic hydrolyzates with Saccharomyces cerevisiae resulted in about 89.7-95.1% of the theoretical ethanol yield after 24h. Therefore, NaOH+UHPE is argued to be a potential alternative technology for pretreatment of bamboo.
authors.affiliation: International Centre for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Zehui
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: International Centre for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Benhua
authors.initials: B
authors.lastname: Fei
authors.affiliation: International Centre for Bamboo and Rattan, Beijing 100102, China. Electronic address: lizq@icbr.ac.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
mesh_terms: D031723:Bambusa / Q000737:chemistry*; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D010316:Particle Size; D058105:Polymerization; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D012972:Sodium Hydroxide / Q000494:pharmacology; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D000431:Ethanol; D012972:Sodium Hydroxide; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose
keywords: Bamboo; Enzymatic hydrolysis; NaOH pretreatment; Ultra-high pressure explosion