title: Assessment of biochemical and immunomodulatory activity of sulphated polysaccharides from Ulva intestinalis.
abstract: The biochemical characteristics and immunomodulatory activity of sulphated polysaccharides isolated from Ulva intestinalis and fractionated using a silica-silica column were investigated. The unfractionated (USP) and fractionated sulphated polysaccharides (FSP4, FSP30, and FSP32) consisted mostly of carbohydrates (4.84-26.55%) and sulphates (2.85-20.42%). Structural analyses showed that USP, FSP4, FSP30 and FSP32 had molecular weights of 300, 80, 110 and 140kDa, respectively. FSP30 exhibited the strongest DPPH radical scavenging activity. Moreover, FSP30 showed stronger immunomodulatory activities than UPS in term of stimulating the production of pro-inflammatory cytokines, including nitric oxide (NO), tumour necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), in macrophage J774A.1 cells. USP and FSP30 were not cytotoxic to mouse macrophage at the tested concentrations (6.25-50μg/mL). The results suggested that U. intestinalis polysaccharides could be explored as potential antioxidant and immunomodulatory agents to be used as complementary medicine or functional foods.
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Takham, Bangkhuntien, Bangkok 10150, Thailand.
authors.forename: Napassorn
authors.initials: N
authors.lastname: Peasura
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Takham, Bangkhuntien, Bangkok 10150, Thailand. Electronic address: nutta.lao@kmutt.ac.th.
authors.forename: Natta
authors.initials: N
authors.lastname: Laohakunjit
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Takham, Bangkhuntien, Bangkok 10150, Thailand.
authors.forename: Orapin
authors.initials: O
authors.lastname: Kerdchoechuen
authors.affiliation: Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Rd., Bang Mod, Thung Khru, Bangkok 10140, Thailand.
authors.forename: Punchira
authors.initials: P
authors.lastname: Vongsawasdi
authors.affiliation: Department of Cosmeceutics, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan, ROC.
authors.forename: Louis Kuoping
authors.initials: LK
authors.lastname: Chao
mesh_terms: D000818:Animals; D002460:Cell Line; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D015846:Monokines / Q000276:immunology* / Q000378:metabolism; D009569:Nitric Oxide / Q000276:immunology* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D044445:Ulva / Q000737:chemistry*
chemical_list: D007155:Immunologic Factors; D015846:Monokines; D011134:Polysaccharides; D009569:Nitric Oxide
keywords: Immunomodulatory activity; Silica-silica column; Sulphated polysaccharide; Ulva intestinalis