title: Reproductive outcomes following preimplantation genetic diagnosis using fluorescence in situ hybridization for 52 translocation carrier couples with a history of recurrent pregnancy loss.
abstract: Forty-six reciprocal and six Robertsonian translocation carrier couples who experienced recurrent pregnancy loss underwent fluorescence in situ hybridization-based preimplantation genetic diagnosis (PGD) for the presence of the two translocated chromosomes. Out of 52 couples, 17 (33%) were undergoing infertility treatment. In total, 239 PGD cycles as oocyte retrieval (OR) were applied. The transferrable rate of negatively diagnosed embryos at the cleavage stage was 26.3%; 71 embryos were transferred as single blastocysts. The clinical pregnancy rate per transfer was 60.6%. We obtained 41 healthy live births with 3 incidences of miscarriage (7.0%). The average cumulative live birth rate was 76.9% during 4.6 OR cycles using a mild ovarian stimulation strategy. The outcomes were classified into four groups based on carrier gender and maternal age (young (<38 years) or advanced). PGD was performed for 52 couples of which the average number of OR cycles was 4.1, 2.1, 6.7 and 4.5 in young female and male carriers and female and male carriers of advanced age; the live birth rate for a primiparity was 77.8, 72.7, 66.7 and 50.0% in those groups. These results suggest that the final live birth rate might be influenced by maternal age regardless of the gender of the carrier.
authors.affiliation: Kato Ladies Clinic, Tokyo, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kato
authors.affiliation: Kato Ladies Clinic, Tokyo, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Aoyama
authors.affiliation: Kato Ladies Clinic, Tokyo, Japan.
authors.forename: Nami
authors.initials: N
authors.lastname: Kawasaki
authors.affiliation: Kato Ladies Clinic, Tokyo, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Kato Ladies Clinic, Tokyo, Japan.
authors.forename: Tang
authors.initials: T
authors.lastname: Xiaohui
authors.affiliation: Shinjuku ART Clinic, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Abe
authors.affiliation: Kato Ladies Clinic, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kuroda
mesh_terms: D000026:Abortion, Habitual / Q000175:diagnosis* / Q000235:genetics* / Q000628:therapy; D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D010062:Ovulation Induction / Q000379:methods; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods; D014178:Translocation, Genetic