title: JAG1-Mediated Notch Signaling Regulates Secretory Cell Differentiation of the Human Airway Epithelium.
abstract: Basal cells (BC) are the stem/progenitor cells of the human airway epithelium capable of differentiating into secretory and ciliated cells. Notch signaling activation increases BC differentiation into secretory cells, but the role of individual Notch ligands in regulating this process in the human airway epithelium is largely unknown. The objective of this study was to define the role of the Notch ligand JAG1 in regulating human BC differentiation. JAG1 over-expression in BC increased secretory cell differentiation, with no effect on ciliated cell differentiation. Conversely, knockdown of JAG1 decreased expression of secretory cell genes. These data demonstrate JAG1-mediated Notch signaling regulates differentiation of BC into secretory cells.
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Gomi
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Staudt
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Salit
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kaner
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Jonna
authors.initials: J
authors.lastname: Heldrich
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Allison M
authors.initials: AM
authors.lastname: Rogalski
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Arbelaez
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA.
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Crystal
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Avenue, Box 164, New York, NY, 10065, USA. geneticmedicine@med.cornell.edu.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Walters
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D000072100:Jagged-1 Protein / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods; D015398:Signal Transduction / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: C000606273:JAG1 protein, human; D000072100:Jagged-1 Protein; D051880:Receptors, Notch
keywords: Basal stem/progenitor cells; Differentiation; Human airway epithelium; JAG1; Notch signaling