title: Increased levels of circulating platelet derived microparticles in Crohn's disease patients.
abstract: OBJECTIVE
  Platelet activation is a consistent feature in inflammatory bowel disease. However, the role of circulating platelet derived microparticles (PDMPs) and the effects of disease activity and treatment on their levels has not been clarified yet in this disorder.
  
  
  METHODS
  Using flow cytometry, we measured platelet derived microparticles and platelet derived microparticles expressing Annexin V in platelet rich plasma from 47 Crohn's disease and 43 ulcerative colitis patients and 24 healthy controls.
  
  
  RESULTS
  Crohn's disease patients have greater PDMPs (0.31% ± 0.07% versus 0.14% ± 0.04%, p = 0.02) and PDMPs expressing Annexin V (27% ± 2.6% versus 14.6% ± 2.7%, p = 0.002) levels in comparison with healthy controls; however, both microparticles levels are not related with disease activity. Crohn's disease patients on 5-ASA therapy show lower levels of PDMPs in comparison with those on no 5-ASA (0.30% ± 0.07% versus 0.32% ± 0.09%, p = 0.048). Ulcerative colitis patients have similar PDMPs and PDMPs expressing Annexin V levels, compared to healthy controls (p = 0.06 and p = 0.2, respectively) and there is no correlation of both microparticles expression with disease activity. 5-ASA has no effect on both microparticles levels in ulcerative colitis patients. Anti-TNF-α treatment has no effect on study's microparticles expression in Crohn's and ulcerative colitis patients.
  
  
  CONCLUSIONS
  Circulating levels of platelet derived microparticles are increased only in Crohn's patients, but they do not correlate with disease activity. 5-ASA treatment is associated with lower levels of PDMPs only in Crohn's, while anti-TNF-α treatment does not influence expression of microparticles in inflammatory bowel disease patients.
authors.affiliation: a Hepatogastroenterology Unit, Second Department of Internal Medicine - Propaedeutic Research Institute and Diabetes Center , "Attikon" University General Hospital , Athens , Greece ;
authors.forename: Georgios
authors.initials: G
authors.lastname: Tziatzios
authors.affiliation: a Hepatogastroenterology Unit, Second Department of Internal Medicine - Propaedeutic Research Institute and Diabetes Center , "Attikon" University General Hospital , Athens , Greece ;
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Polymeros
authors.affiliation: b Laboratory of Cytopathology , Medical School, National and Kapodistrian University, "Attikon" University General Hospital , Athens , Greece.
authors.forename: Aris
authors.initials: A
authors.lastname: Spathis
authors.affiliation: a Hepatogastroenterology Unit, Second Department of Internal Medicine - Propaedeutic Research Institute and Diabetes Center , "Attikon" University General Hospital , Athens , Greece ;
authors.forename: Maria
authors.initials: M
authors.lastname: Triantafyllou
authors.affiliation: a Hepatogastroenterology Unit, Second Department of Internal Medicine - Propaedeutic Research Institute and Diabetes Center , "Attikon" University General Hospital , Athens , Greece ;
authors.forename: Paraskevas
authors.initials: P
authors.lastname: Gkolfakis
authors.affiliation: b Laboratory of Cytopathology , Medical School, National and Kapodistrian University, "Attikon" University General Hospital , Athens , Greece.
authors.forename: Petros
authors.initials: P
authors.lastname: Karakitsos
authors.affiliation: a Hepatogastroenterology Unit, Second Department of Internal Medicine - Propaedeutic Research Institute and Diabetes Center , "Attikon" University General Hospital , Athens , Greece ;
authors.forename: George
authors.initials: G
authors.lastname: Dimitriadis
authors.affiliation: a Hepatogastroenterology Unit, Second Department of Internal Medicine - Propaedeutic Research Institute and Diabetes Center , "Attikon" University General Hospital , Athens , Greece ;
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Triantafyllou
mesh_terms: D000328:Adult; D017304:Annexin A5 / Q000378:metabolism; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles; D003093:Colitis, Ulcerative / Q000097:blood; D003424:Crohn Disease / Q000097:blood* / Q000188:drug therapy*; D005260:Female; D005434:Flow Cytometry; D006115:Greece; D006801:Humans; D016014:Linear Models; D008297:Male; D019804:Mesalamine / Q000627:therapeutic use*; D008875:Middle Aged; D015539:Platelet Activation / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
chemical_list: D017304:Annexin A5; D000894:Anti-Inflammatory Agents, Non-Steroidal; D014409:Tumor Necrosis Factor-alpha; D019804:Mesalamine
keywords: 5-ASA; anti-TNF-α; inflammatory bowel disease; microparticles