title: How Diet Intervention via Modulation of DNA Damage Response through MicroRNAs May Have an Effect on Cancer Prevention and Aging, an in Silico Study.
abstract: The DNA damage response (DDR) is a molecular mechanism that cells have evolved to sense DNA damage (DD) to promote DNA repair, or to lead to apoptosis, or cellular senescence if the damage is too extensive. Recent evidence indicates that microRNAs (miRs) play a critical role in the regulation of DDR. Dietary bioactive compounds through miRs may affect activity of numerous genes. Among the most studied bioactive compounds modulating expression of miRs are epi-gallocatechin-3-gallate, curcumin, resveratrol and n3-polyunsaturated fatty acids. To compare the impact of these dietary compounds on DD/DDR network modulation, we performed a literature search and an in silico analysis by the DIANA-mirPathv3 software. The in silico analysis allowed us to identify pathways shared by different miRs involved in DD/DDR vis-à-vis the specific compounds. The results demonstrate that certain miRs (e.g., -146, -21) play a central role in the interplay among DD/DDR and the bioactive compounds. Furthermore, some specific pathways, such as "fatty acids biosynthesis/metabolism", "extracellular matrix-receptor interaction" and "signaling regulating the pluripotency of stem cells", appear to be targeted by most miRs affected by the studied compounds. Since DD/DDR and these pathways are strongly related to aging and carcinogenesis, the present in silico results of our study suggest that monitoring the induction of specific miRs may provide the means to assess the antiaging and chemopreventive properties of particular dietary compounds.
authors.affiliation: Diagnostics and Metrology (FSN-TECFIS-DIM), ENEA, C.R. Frascati, Rome 00044, Italy. carotenuto@med.uniroma2.it.|Department Clinical Sciences and Translational Medicine, University ofRome Tor Vergata, Rome 00133, Italy. carotenuto@med.uniroma2.it.
authors.forename: Felicia
authors.initials: F
authors.lastname: Carotenuto
authors.affiliation: Department of Biomolecular Sciences, University of Urbino "Carlo Bo", Urbino 61029, Italy. maria.albertini@uniurb.it.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Albertini
authors.affiliation: Department of Biological Adaptation and Aging B2A, University Pierre et Marie Curie Paris 06, Paris 75252, France. dario.coletti@upmc.fr.
authors.forename: Dario
authors.initials: D
authors.lastname: Coletti
authors.affiliation: Diagnostics and Metrology (FSN-TECFIS-DIM), ENEA, C.R. Frascati, Rome 00044, Italy. vilmercati.ale@tiscali.it.|Fondazione San Raffaele, Ceglie Messapica, Brindisi 72013, Italy. vilmercati.ale@tiscali.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Vilmercati
authors.affiliation: Department of Chemistry, University of Rome "Sapienza", Rome, 00185, Italy. luigi.campanella@uniroma1.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Campanella
authors.affiliation: Department of Pathology, New York Medical College, Valhalla, NY 10595, USA. z_darzynkiewicz@nymc.edu.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Darzynkiewicz
authors.affiliation: Diagnostics and Metrology (FSN-TECFIS-DIM), ENEA, C.R. Frascati, Rome 00044, Italy. laura.teodori@enea.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Teodori
mesh_terms: D000375:Aging / Q000378:metabolism*; D003198:Computer Simulation; D004249:DNA Damage; D004032:Diet; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism / Q000517:prevention & control; D012334:RNA, Neoplasm / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D012334:RNA, Neoplasm
keywords: bioactive compounds; chemoprevention; curcumin; epi-gallocatechin-3gallate; food; n3-PUFA; resveratrol