title: Regenerative effect of hOPG gene-modified autologous PDLs in combination with cell transplantation on periodontal defection in beagle dogs.
abstract: This study evaluated the ability of human osteoprotegerin gene-modified autologous periodontal ligament cells (PDLCs) in combination with cell transplantation to promote periodontal regeneration in beagle dogs. Adenovirus Ad5-hOPG-EGFP-transfected PDLCs and BME-10X collagen membranes were fabricated and used for periodontal repair. Buccal periodontal defects (mesiodistal width × depth: 5 × 5 mm) were created on the second, third, and fourth mandibular premolars in six normal beagle dogs, and the defects were histologically and histomorphometrically assessed for periodontal regeneration in the following four groups: (1) hOPG-PDLCs + BME-10X, (2) mock-PDLCs + BME-10X, (3) PDLCs + BME-10X, and (4) BME-10X. The radiographic and histological results suggested that hOPG-PDLCs significantly promoted periodontal defect repair. This study demonstrates the potential of hOPG-modified PDLCs for periodontal tissue regeneration.
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China.
authors.forename: Su
authors.initials: S
authors.lastname: Jiang
authors.affiliation: School of Stomatology Fujian Medical University, Fuzhou, 350002, Fujian, China.
authors.forename: Kunqi
authors.initials: K
authors.lastname: Tang
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China. fhyan2005@126.com.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Yan
keywords: Gene therapy; OPG; Periodontal tissue defects; Tissue regeneration