title: Clinical effect of photodynamic therapy on primary carious dentin after partial caries removal.
abstract: This study was conducted to assess the clinical effect of photodynamic therapy (PDT) in the decontamination of the deep dentin of deciduous molars submitted to partial removal of carious tissue. After cavity preparation, dentin samples were taken from the pulp wall of nineteen deciduous molars before and after PDT application. Remaining dentin was treated with 0.01% methylene blue dye followed by irradiation with an InGaAlP diode laser (λ - 660 nm; 40 mW; 120 J/cm2; 120 s). Dentin samples were microbiologically assessed for the enumeration of total microorganisms, Lactobacillus spp. and mutans streptococci. There was no significant difference in the number of colony-forming units (CFU) for any of the microorganisms assessed (p > 0.05). Photodynamic therapy, using 0.01% methylene blue dye at a dosimetry of 120 J/cm2 would not be a viable clinical alternative to reduce bacterial contamination in deep dentin.
authors.affiliation: Universidade Federal do Maranhão - UFMA, School of Dentistry, Postgraduate Program in Dentistry, São Luís, MA, Brazil.
authors.forename: Pierre Adriano Moreno
authors.initials: PA
authors.lastname: Neves
authors.affiliation: Universidade Federal do Maranhão - UFMA, School of Dentistry, Postgraduate Program in Dentistry, São Luís, MA, Brazil.
authors.forename: Leonardo Abrantes
authors.initials: LA
authors.lastname: Lima
authors.affiliation: Universidade Federal do Maranhão - UFMA, School of Dentistry, São Luís, MA, Brazil.
authors.forename: Fernanda Cristina Nogueira
authors.initials: FC
authors.lastname: Rodrigues
authors.affiliation: Universidade Federal do Maranhão - UFMA, School of Dentistry, Department of Dentistry II, São Luís, MA, Brazil.
authors.forename: Tarcisio Jorge
authors.initials: TJ
authors.lastname: Leitão
authors.affiliation: Universidade Federal do Maranhão - UFMA, School of Dentistry, Department of Dentistry II, São Luís, MA, Brazil.
authors.forename: Cecília Cláudia Costa
authors.initials: CC
authors.lastname: Ribeiro
mesh_terms: D002648:Child; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000188:drug therapy*; D003804:Dentin / Q000187:drug effects* / Q000382:microbiology / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects / Q000528:radiation effects; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D008297:Male; D008751:Methylene Blue / Q000008:administration & dosage*; D010778:Photochemotherapy / Q000379:methods*; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors; D014094:Tooth, Deciduous / Q000382:microbiology; D016896:Treatment Outcome
chemical_list: D004791:Enzyme Inhibitors; D008751:Methylene Blue