title: Evaluation of Leishmania (Leishmania) infantum excreted-secreted antigens for detection of canine leishmaniasis.
abstract: The efficacy of tests with L. (L.) infantum excreted-secreted antigens (ESA) to detect canine leishmaniasis (CanL) was evaluated using immunoblotting (ESA-blot), ELISA (ESA-ELISA) and ELISA with alkaline extract from promastigotes (PAE). Of one hundred fifty-five domestic dogs tested, 100 were suspected of CanL, 23 had other diseases and 32 were healthy. Sera from the dogs suspected of CanL were tested by immunohistochemistry (IHC), and 54% were confirmed to be infected by L. (L.) infantum (38 symptomatic and 16 asymptomatic). Of these, 100% were positive by ESA-blot, ESA-ELISA and PAE-ELISA. In the ESA-blot their sera recognized polypeptides in the 26.5-31.5kDa region. Of the 46% of dogs with negative IHC, 44-53% tested positive in all three tests irrespective of clinical status. The twenty-three dogs with other diseases were negative by ESA-blot, but sera from 9% and 26% of them reacted with ESA-ELISA and PAE-ELISA, respectively. The 32 healthy dogs were negative in all the tests. ESA-blot showed good correlation with IHC in the detection of CanL and a high specificity index.
authors.affiliation: Instituto de Medicina Tropical de São Paulo da Universidade de São Paulo (USP), SP, Brazil. Electronic address: vivi_vane@hotmail.com.
authors.forename: Viviana
authors.initials: V
authors.lastname: Pinedo-Cancino
authors.affiliation: Faculdade de Medicina da USP, Departamento de Patologia, SP, Brazil. Electronic address: mdlauren@usp.br.
authors.forename: Márcia Dalastra
authors.initials: MD
authors.lastname: Laurenti
authors.affiliation: Instituto de Medicina Tropical de São Paulo da Universidade de São Paulo (USP), SP, Brazil. Electronic address: nkesper@usp.br.
authors.forename: Norival
authors.initials: N
authors.lastname: Kesper
authors.affiliation: Instituto de Medicina Tropical de São Paulo da Universidade de São Paulo (USP), SP, Brazil; Faculdade de Medicina da USP, Departamento de Medicina Preventiva, SP, Brazil. Electronic address: eumezawa@usp.br.
authors.forename: Eufrosina Setsu
authors.initials: ES
authors.lastname: Umezawa
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015151:Immunoblotting; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000276:immunology* / Q000662:veterinary*; D008297:Male; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan
keywords: Canine leishmaniasis; ELISA; Excreted antigens; Immunoblotting; Leishmania infantum; Secreted antigens