title: Confocal Bioluminescence Imaging for Living Tissues with a Caged Substrate of Luciferin.
abstract: Fluorescence imaging can elucidate morphological organization and coordinal networks, but its background luminescence degrades the image contrast. Our confocal bioluminescence imaging system uses a luciferase caged substrate, with light passing through multipinhole arrays, causing bioluminescence at a focal plane. After a charge-coupled device camera captures luminescence, the imaging system acquires confocal images of multilayered cells with depth information, supporting quantitative analysis of spatial cellular localization in living tissues.
authors.affiliation: Department of Chemistry, School of Science, ‡Graduate School of Pharmaceutical Sciences, and §Graduate School of Medicine, The University of Tokyo , 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Department of Chemistry, School of Science, ‡Graduate School of Pharmaceutical Sciences, and §Graduate School of Medicine, The University of Tokyo , 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Kawamura
authors.affiliation: Department of Chemistry, School of Science, ‡Graduate School of Pharmaceutical Sciences, and §Graduate School of Medicine, The University of Tokyo , 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kojima
authors.affiliation: Department of Chemistry, School of Science, ‡Graduate School of Pharmaceutical Sciences, and §Graduate School of Medicine, The University of Tokyo , 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan.
authors.forename: Mako
authors.initials: M
authors.lastname: Kamiya
authors.affiliation: Department of Chemistry, School of Science, ‡Graduate School of Pharmaceutical Sciences, and §Graduate School of Medicine, The University of Tokyo , 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.affiliation: Department of Chemistry, School of Science, ‡Graduate School of Pharmaceutical Sciences, and §Graduate School of Medicine, The University of Tokyo , 7-3-1 Bunkyo-ku, Hongo, Tokyo 113-0033, Japan.
authors.forename: Takeaki
authors.initials: T
authors.lastname: Ozawa
mesh_terms: D000818:Animals; D002460:Cell Line; D049454:Firefly Luciferin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D041681:NIH 3T3 Cells; D036103:Nanotechnology
chemical_list: D049454:Firefly Luciferin; D008156:Luciferases