title: The Inhibition of Mast Cell Activation of Radix Paeoniae alba Extraction Identified by TCRP Based and Conventional Cell Function Assay Systems.
abstract: Chinese herbs have long been used to treat allergic disease, but recently the development was greatly impeded by the lack of good methods to explore the mechanism of action. Here, we showed the effects of Chinese herb Radix Paeoniae alba were identified and characterized by a mast cell activation assay that involves electronic impedance readouts for dynamic monitoring of cellular responses to produce time-dependent cell responding profiles (TCRPs), and the anti-allergic activities were further confirmed with various conventional molecular and cell biology tools. We found Radix P. alba can dose-dependently inhibit TCPRs, and have anti-allergic function in vitro and in vivo. Radix P. alba suppressed mast cell degranulation not only inhibiting the translocation of granules to the plasma membrane, but also blocking membrane fusion and exocytosis; and that there may be other anti-allergic components in addition to paeoniflorin. Our results suggest that Radix P. alba regulated mast cell activation with multiple targets, and this approach is also suitable for discovering other mast cell degranulation-targeting Chinese herbs and their potential multi-target mechanisms.
authors.affiliation: Program in Molecular Cell Biology, Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou, China.|Central Laboratory, The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Fu
authors.affiliation: Program in Molecular Cell Biology, Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.affiliation: School of Pharmacy, Nanjing Chinese Medical University, Nanjing, China.
authors.forename: Xingde
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Experimental Animal Research Center, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Tu
authors.affiliation: Program in Molecular Cell Biology, Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Mingjiao
authors.initials: M
authors.lastname: Sun
authors.affiliation: Program in Molecular Cell Biology, Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Mou
authors.affiliation: Experimental Animal Research Center, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Qiyang
authors.initials: Q
authors.lastname: Shou
authors.affiliation: Program in Molecular Cell Biology, Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Yuehai
authors.initials: Y
authors.lastname: Ke
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism; D005089:Exocytosis; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008561:Membrane Fusion; D051379:Mice; D008813:Mice, Inbred ICR; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D018926:Anti-Allergic Agents; D010936:Plant Extracts