title: Age, serum 25-hydroxyvitamin D and vitamin D receptor (VDR) expression and function in peripheral blood mononuclear cells.
abstract: The relationship between age, vitamin D status, expression and functionality of the vitamin D receptor (VDR), and key genes in the vitamin D pathway in immune cells is unclear. We enrolled adults 50 to 69 years old (20 subjects) and 70+ (20 subjects) and measured: 1) 25(OH)D levels by liquid chromatography/mass spectrometry; and 2) mRNA expression of VDR, 1α-OHase, 1,25D3-MARRS, TREM-1, cathelicidin, RIG-I, and interferon-β by qRT-PCR. Mean serum 25(OH)D was 30 ± 4 ng/mL and was not associated with age. Baseline expression of VDR, 1α-OHase, 1,25D3-MARRS, TREM-1, and RIG-I also did not differ by age; IFN-β expression, however, was higher in the 70+ year old group. 25(OH)D3- and 1,25(OH)2D3-induced VDR, TREM-1 and cathelicidin expression were similar between age groups, as was LPS-induced expression of VDR and of 1α-OHase. Ligand-induced 1,25D3-MARRS expression was higher in subjects ≥ 70 years. Serum 25(OH)D was inversely associated with LPS-stimulated VDR expression and with baseline or vitamin D-induced TREM-1 expression, adjusting for age, self-rated health, and functional status. In healthy adults ≥ 50 years, the expression and functionality of the VDR, 1α-OHase and key vitamin D pathway genes were not consistently associated with age.
authors.affiliation: Abbott Nutrition, Columbus, OH, USA.|Marshfield Clinic, Marshfield, WI, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Coleman
authors.affiliation: Battelle, Columbus, OH, USA.
authors.forename: Margarita
authors.initials: M
authors.lastname: Mishina
authors.affiliation: U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Thompson
authors.affiliation: Battelle, Columbus, OH, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Spencer
authors.affiliation: U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Reber
authors.affiliation: U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: William G
authors.initials: WG
authors.lastname: Davis
authors.affiliation: U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Po-Yung
authors.initials: PY
authors.lastname: Cheng
authors.affiliation: Marshfield Clinic, Marshfield, WI, USA.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Belongia
authors.affiliation: Vanderbilt University, Nashville, TN, USA.
authors.forename: H Keipp
authors.initials: HK
authors.lastname: Talbot
authors.affiliation: Marshfield Clinic, Marshfield, WI, USA.|University of Minnesota School of Public Health, Minneapolis, MN, USA.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Sundaram
authors.affiliation: Vanderbilt University, Nashville, TN, USA.
authors.forename: Marie R
authors.initials: MR
authors.lastname: Griffin
authors.affiliation: U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: David K
authors.initials: DK
authors.lastname: Shay
authors.affiliation: U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Suryaprakash
authors.initials: S
authors.lastname: Sambhara
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000378:metabolism*; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism; D002853:Chromatography, Liquid; D000071457:DEAD Box Protein 58 / Q000378:metabolism; D005260:Female; D006801:Humans; D016899:Interferon-beta / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000378:metabolism*; D011971:Receptors, Immunologic; D015398:Signal Transduction; D000073871:Triggering Receptor Expressed on Myeloid Cells-1 / Q000378:metabolism; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; C579457:VDR protein, human; D014807:Vitamin D; D016899:Interferon-beta; C104450:25-hydroxyvitamin D; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; C000600378:CYP27B1 protein, human; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human; D054804:Cathelicidins
keywords: 1α-hydroxylase; Gerotarget; PBMC; mRNA expression; vitamin D; vitamin D receptor