title: Probing protein complexes inside living cells using a silicon nanowire-based pull-down assay.
abstract: Most proteins perform their functions as interacting complexes. Here we propose a novel method for capturing an intracellular protein and its interacting partner out of living cells by utilizing intracellular access of antibody modified vertical silicon nanowire arrays whose surface is covered with a polyethylene glycol layer to prevent strong cell adhesion. Such a feature facilitates the removal of cells by simple washing, enabling subsequent detection of a pulled-down protein and its interacting partner, and further assessment of a drug-induced change in the interacting complex. Our new SiNW-based tool is thus suitable for authentication of protein networks inside living cells.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. eunyang@kist.re.kr soyeonkim@kist.re.kr.
authors.forename: Sojoong
authors.initials: S
authors.lastname: Choi
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. eunyang@kist.re.kr soyeonkim@kist.re.kr.
authors.forename: Hyunju
authors.initials: H
authors.lastname: Kim
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. eunyang@kist.re.kr soyeonkim@kist.re.kr and Department of Biomedical Engineering, Korea University of Science and Technology (UST), KIST campus, Seoul 02792, Republic of Korea.
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. eunyang@kist.re.kr soyeonkim@kist.re.kr and Department of Biological Chemistry, Korea University of Science and Technology (UST), KIST campus, Seoul 02792, Republic of Korea.
authors.forename: Eun Gyeong
authors.initials: EG
authors.lastname: Yang
mesh_terms: D002448:Cell Adhesion; D006801:Humans; D020014:K562 Cells; D053770:Nanowires; D025941:Protein Interaction Mapping; D011506:Proteins / Q000032:analysis*; D012825:Silicon
chemical_list: D011506:Proteins; D012825:Silicon