title: Evaluation of Molecular Methods for Identification of Salmonella Serovars.
abstract: Classification by serotyping is the essential first step in the characterization of Salmonella isolates and is important for surveillance, source tracking, and outbreak detection. To improve detection and reduce the burden of salmonellosis, several rapid and high-throughput molecular Salmonella serotyping methods have been developed.The aim of this study was to compare three commercial kits, Salm SeroGen (Salm Sero-Genotyping AS-1 kit), Check&Trace (Check-Points), and xMAP (xMAP Salmonella serotyping assay), to the Salmonella genoserotyping array (SGSA) developed by our laboratory. They were assessed using a panel of 321 isolates that represent commonly reported serovars from human and nonhuman sources globally. The four methods correctly identified 73.8% to 94.7% of the isolates tested. The methods correctly identified 85% and 98% of the clinically important Salmonella serovars Enteritidis and Typhimurium, respectively. The methods correctly identified 75% to 100% of the nontyphoidal, broad host range Salmonella serovars, including Heidelberg, Hadar, Infantis, Kentucky, Montevideo, Newport, and Virchow. The sensitivity and specificity of Salmonella serovars Typhimurium and Enteritidis ranged from 85% to 100% and 99% to 100%, respectively.It is anticipated that whole-genome sequencing will replace serotyping in public health laboratories in the future. However, at present, it is approximately three times more expensive than molecular methods. Until consistent standards and methodologies are deployed for whole-genome sequencing, data analysis and interlaboratory comparability remain a challenge. The use of molecular serotyping will provide a valuable high-throughput alternative to traditional serotyping. This comprehensive analysis provides a detailed comparison of commercial kits available for the molecular serotyping of Salmonella.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada catherine.yoshida@canada.ca.
authors.forename: Catherine
authors.initials: C
authors.lastname: Yoshida
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.
authors.forename: Simone
authors.initials: S
authors.lastname: Gurnik
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.
authors.forename: Aaminah
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.
authors.forename: Travis
authors.initials: T
authors.lastname: Blimkie
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Murphy
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Toronto, Ontario, Canada.
authors.forename: John H E
authors.initials: JH
authors.lastname: Nash
mesh_terms: D000818:Animals; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012475:Salmonella / Q000145:classification* / Q000235:genetics; D012480:Salmonella Infections / Q000175:diagnosis / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000382:microbiology*; D065288:Serogroup; D012703:Serotyping / Q000379:methods*