title: A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize.
abstract: Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants, and among these, the tripartite viruses Brome mosaic virus and Cucumber mosaic virus have been shown to induce VIGS in maize (Zea mays). We describe here a new DNA-based VIGS system derived from Foxtail mosaic virus (FoMV), a monopartite virus that is able to establish systemic infection and silencing of endogenous maize genes homologous to gene fragments inserted into the FoMV genome. To demonstrate VIGS applications of this FoMV vector system, four genes, phytoene desaturase (functions in carotenoid biosynthesis), lesion mimic22 (encodes a key enzyme of the porphyrin pathway), iojap (functions in plastid development), and brown midrib3 (caffeic acid O-methyltransferase), were silenced and characterized in the sweet corn line Golden × Bantam. Furthermore, we demonstrate that the FoMV infectious clone establishes systemic infection in maize inbred lines, sorghum (Sorghum bicolor), and green foxtail (Setaria viridis), indicating the potential wide applications of this viral vector system for functional genomics studies in maize and other monocots.
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa 50011 (Y.M., B.M.K., J.H.H., S.A.W.);Department of Agriculture, Alcorn State University, Lorman, Mississippi 39096 (C.Z.); andDuPont Pioneer, Johnston, Iowa 50131 (B.M.K.).
authors.forename: Yu
authors.initials: Y
authors.lastname: Mei
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa 50011 (Y.M., B.M.K., J.H.H., S.A.W.);Department of Agriculture, Alcorn State University, Lorman, Mississippi 39096 (C.Z.); andDuPont Pioneer, Johnston, Iowa 50131 (B.M.K.).
authors.forename: Chunquan
authors.identifier: 0000-0001-9887-9209
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa 50011 (Y.M., B.M.K., J.H.H., S.A.W.);Department of Agriculture, Alcorn State University, Lorman, Mississippi 39096 (C.Z.); andDuPont Pioneer, Johnston, Iowa 50131 (B.M.K.).
authors.forename: Bliss M
authors.initials: BM
authors.lastname: Kernodle
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa 50011 (Y.M., B.M.K., J.H.H., S.A.W.);Department of Agriculture, Alcorn State University, Lorman, Mississippi 39096 (C.Z.); andDuPont Pioneer, Johnston, Iowa 50131 (B.M.K.).
authors.forename: John H
authors.initials: JH
authors.lastname: Hill
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, Iowa 50011 (Y.M., B.M.K., J.H.H., S.A.W.);Department of Agriculture, Alcorn State University, Lorman, Mississippi 39096 (C.Z.); andDuPont Pioneer, Johnston, Iowa 50131 (B.M.K.) swhitham@iastate.edu.
authors.forename: Steven A
authors.identifier: 0000-0003-3542-3188
authors.initials: SA
authors.lastname: Whitham
mesh_terms: D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D010940:Plant Proteins / Q000235:genetics; D017863:Potexvirus / Q000235:genetics* / Q000502:physiology; D032983:Setaria Plant / Q000235:genetics* / Q000821:virology; D045868:Sorghum / Q000235:genetics* / Q000821:virology; D003313:Zea mays / Q000235:genetics* / Q000821:virology
chemical_list: D010940:Plant Proteins