title: Silicon nanowire based biosensing platform for electrochemical sensing of Mebendazole drug activity on breast cancer cells.
abstract: Electrochemical approaches have played crucial roles in bio sensing because of their Potential in achieving sensitive, specific and low-cost detection of biomolecules and other bio evidences. Engineering the electrochemical sensing interface with nanomaterials tends to new generations of label-free biosensors with improved performances in terms of sensitive area and response signals. Here we applied Silicon Nanowire (SiNW) array electrodes (in an integrated architecture of working, counter and reference electrodes) grown by low pressure chemical vapor deposition (LPCVD) system with VLS procedure to electrochemically diagnose the presence of breast cancer cells as well as their response to anticancer drugs. Mebendazole (MBZ), has been used as antitubulin drug. It perturbs the anodic/cathodic response of the cell covered biosensor by releasing Cytochrome C in cytoplasm. Reduction of cytochrome C would change the ionic state of the cells monitored by SiNW biosensor. By applying well direct bioelectrical contacts with cancer cells, SiNWs can detect minor signal transduction and bio recognition events, resulting in precise biosensing. Our device detected the trace of MBZ drugs (with the concentration of 2nM) on electrochemical activity MCF-7 cells. Also, experimented biological analysis such as confocal and Flowcytometry assays confirmed the electrochemical results.
authors.affiliation: Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran.
authors.forename: Hani
authors.initials: H
authors.lastname: Shashaani
authors.affiliation: Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Faramarzpour
authors.affiliation: Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hassanpour
authors.affiliation: Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Namdar
authors.affiliation: Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Alikhani
authors.affiliation: Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Nano Electronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Eng, University of Tehran, P.O. Box 14395/515, Tehran, Iran. Electronic address: m.abdolahad@ut.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdolahad
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D001940:Breast / Q000187:drug effects / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D045304:Cytochromes c / Q000032:analysis / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008463:Mebendazole / Q000494:pharmacology*; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D012825:Silicon / Q000737:chemistry*; D050257:Tubulin Modulators / Q000494:pharmacology*
chemical_list: D050257:Tubulin Modulators; D008463:Mebendazole; D045304:Cytochromes c; D012825:Silicon
keywords: Biosensor; Cancer cell; Cyclic voltammetry; Electrochemical response; Mebendazole drug; Silicon nanowire