title: Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model.
abstract: Neuregulin 1 (Nrg1) is involved in multiple biological processes in the nervous system. The present study investigated changes in Nrg1 signaling in the major brain regions of mice subjected to lipopolysaccharide (LPS)-induced neuroinflammation. At 24 h post‑intraperitoneal injection of LPS, mouse brain tissues, including tissues from the cortex, striatum, hippocampus and hypothalamus, were collected. Reverse transcription‑polymerase chain reaction was used to determine the expression of Nrg1 and its receptors, Neu and ErbB4, at the mRNA level. Western blotting was performed to determine the levels of these proteins and the protein levels of phosphorylated extracellular signal-regulated kinases (Erk)1/2 and Akt1. Immunohistochemical staining was utilized to detect the levels of pNeu and pErbB4 in these regions. LPS successfully induced sites of neuroinflammation in these regions, in which changes in Nrg1, Neu and ErbB4 at the mRNA and protein levels were identified compared with controls. LPS induced a reduction in pNeu and pErbB4 in the striatum and hypothalamus, although marginally increased pErbB4 levels were found in the hippocampus. LPS increased the overall phosphorylation of Src but this effect was reduced in the hypothalamus. Moreover, increased phosphorylation of Akt1 was found in the striatum and hippocampus. These data suggest diverse roles for Nrg1 signaling in these regions during the process of neuroinflammation.
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Zhai
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Shuang-Xi
authors.initials: SX
authors.lastname: Chen
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Hu
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Hui-Fan
authors.initials: HF
authors.lastname: Shen
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Pei-Zhi
authors.initials: PZ
authors.lastname: Huang
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Xu
authors.affiliation: Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Mei
authors.affiliation: Department of Neurology, The 2nd Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Ben-Ping
authors.initials: BP
authors.lastname: Zhang
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Wei-Jiang
authors.initials: WJ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D004660:Encephalitis / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000009:adverse effects*; D051379:Mice; D020890:Neuregulin-1 / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D066247:Receptor, ErbB-4 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D008070:Lipopolysaccharides; D020890:Neuregulin-1; D051057:Proto-Oncogene Proteins c-akt; D018719:Erb-b2 Receptor Tyrosine Kinases; D066247:Receptor, ErbB-4