title: Drosophila Pkaap regulates Rab4/Rab11-dependent traffic and Rab11 exocytosis of innate immune cargo.
abstract: The secretion of immune-mediators is a critical step in the host innate immune response to pathogen invasion, and Rab GTPases have an important role in the regulation of this process. Rab4/Rab11 recycling endosomes are involved in the sorting of immune-mediators into specialist Rab11 vesicles that can traffic this cargo to the plasma membrane; however, how this sequential delivery process is regulated has yet to be fully defined. Here, we report that Drosophila Pkaap, an orthologue of the human dual-specific A-kinase-anchoring protein 2 or D-AKAP2 (also called AKAP10), appeared to have a nucleotide-dependent localisation to Rab4 and Rab11 endosomes. RNAi silencing of pkaap altered Rab4/Rab11 recycling endosome morphology, suggesting that Pkaap functions in cargo sorting and delivery in the secretory pathway. The depletion of pkaap also had a direct effect on Rab11 vesicle exocytosis and the secretion of the antimicrobial peptide Drosomycin at the plasma membrane. We propose that Pkaap has a dual role in antimicrobial peptide traffic and exocytosis, making it an essential component for the secretion of inflammatory mediators and the defence of the host against pathogens.
authors.affiliation: Sansom Institute for Health Research, University of South Australia, Adelaide, South Australia 5001, Australia.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Sorvina
authors.affiliation: Sansom Institute for Health Research, University of South Australia, Adelaide, South Australia 5001, Australia.
authors.forename: Tetyana
authors.initials: T
authors.lastname: Shandala
authors.affiliation: Sansom Institute for Health Research, University of South Australia, Adelaide, South Australia 5001, Australia Doug.Brooks@unisa.edu.au.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Brooks
keywords: Antimicrobial peptide Drosomycin; Drosophila; Endosomes; Innate immunity; Pkaap; Rab11; Rab4