title: The Role of ΔFosB on the Pro-survival Effect of PTHrP in Goat Mammary Epithelial Cells.
abstract: The mechanism of regulation mammary epithelial cell number in ruminant is not fully understood, but is thought to be dependent on the balance of cell proliferation and cell apoptosis. Parathyroid hormone-related protein (PTHrP) could express in mammary epithelial cells and breast cancer cells, and has been reported to regulate cell survival. Here, we showed that PTHrP induced cell proliferation and increased the expression of CyclinD1 and proliferating cell nuclear antigen (PCNA) in goat mammary epithelial cells (GMEC). PTHrP increased the mRNA levels of anti-apoptosis genes Bcl-2 and Bcl-xl, and protected GMEC from apoptosis. We also found ΔFosB, an alternative splicing of finkel-biskis-jinkins murine osteosarcoma B (fosB), inhibited GMEC apoptosis, and induced cell proliferation with increased Bcl-2/Bax and Bcl-xl/Bax ratios. Interestingly, ΔFosB could further promote the pro-survival effect of PTHrP, and the Bcl-2/Bax and Bcl-xl/Bax ratios showed higher levels. We conclude that the pro-survival role of PTHrP in GMEC may be regulated by ΔFosB.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Yao
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Li
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China. zhenghuiling@nwsuaf.edu.cn.
authors.forename: Huiling
authors.initials: H
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006041:Goats; D008321:Mammary Glands, Animal / Q000166:cytology*; D044162:Parathyroid Hormone-Related Protein / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*
chemical_list: D044162:Parathyroid Hormone-Related Protein; D016760:Proto-Oncogene Proteins c-fos
keywords: Apoptosis; Goat; PTHrP; Proliferation; ΔFosB