title: Occurrence of bacteria producing broad-spectrum beta-lactamases and qnr genes in hospital and urban wastewater samples.
abstract: The aims were to investigate the level of antibiotic-resistant bacteria in hospital and urban wastewater and to determine the similarity of isolates obtained from wastewater and hospitalized patients. Wastewater samples were collected in September 2013 and 2014. After identification using MALDI-TOF MS, beta-lactamase production was determined by relevant phenotypic tests. Genes responsible for the production of single beta-lactamase groups and Qnr proteins were established. The epidemiological relationship of the isolates from wastewater and hospitalized patients was determined by PFGE. A total of 51 isolates of enterobacteria were obtained. Overall, 45.1% of them produced broad-spectrum beta-lactamases. Genes encoding TEM, SHV, CTX-M, CIT, DHA and EBC types of enzymes and Qnr proteins were detected. No broad-spectrum beta-lactamase production was confirmed in the urban wastewater treatment plant. The most important finding was the detection of two identical isolates of K. pneumoniae in 2013, one from a patient's urinary catheter and the other from a wastewater sample.
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic.|Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacký
authors.forename: Magdaléna
authors.initials: M
authors.lastname: Röderová
authors.affiliation: Department of Microbiology, University Hospital Olomouc, Czech Republic.
authors.forename: Miroslava Htoutou
authors.initials: MH
authors.lastname: Sedláková
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic.
authors.forename: Vendula
authors.initials: V
authors.lastname: Pudová
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Hricová
authors.affiliation: Department of Microbiology, University Hospital Olomouc, Czech Republic.
authors.forename: Romana
authors.initials: R
authors.lastname: Silová
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic.
authors.forename: Peter Eghonghon Odion
authors.initials: PE
authors.lastname: Imwensi
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic.|Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacký
authors.forename: Jan
authors.initials: J
authors.lastname: Bardoň
authors.affiliation: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic.|Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacký
authors.forename: Milan
authors.initials: M
authors.lastname: Kolář
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D002947:Cities; D024881:Drug Resistance, Bacterial / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006761:Hospitals; D062065:Wastewater / Q000382:microbiology*; D014871:Water Microbiology; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D062065:Wastewater; D001618:beta-Lactamases
keywords: Broad-spectrum beta-lactamases; Enterobacteria; Genetic analysis; Wastewater