title: Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions.
abstract: Plant roots can regenerate after excision of their tip, including the stem cell niche. To determine which developmental program mediates such repair, we applied a combination of lineage tracing, single-cell RNA sequencing, and marker analysis to test different models of tissue reassembly. We show that multiple cell types can reconstitute stem cells, demonstrating the latent potential of untreated plant cells. The transcriptome of regenerating cells prior to stem cell activation resembles that of an embryonic root progenitor. Regeneration defects are more severe in embryonic than in adult root mutants. Furthermore, the signaling domains of the hormones auxin and cytokinin mirror their embryonic dynamics and manipulation of both hormones alters the position of new tissues and stem cell niche markers. Our findings suggest that plant root regeneration follows, on a larger scale, the developmental stages of embryonic patterning and is guided by spatial information provided by complementary hormone domains.
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.
authors.forename: Idan
authors.initials: I
authors.lastname: Efroni
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.
authors.forename: Alison
authors.initials: A
authors.lastname: Mello
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.
authors.forename: Tal
authors.initials: T
authors.lastname: Nawy
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.
authors.forename: Pui-Leng
authors.initials: PL
authors.lastname: Ip
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.
authors.forename: Ramin
authors.initials: R
authors.lastname: Rahni
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: DelRose
authors.affiliation: New York Genome Center, New York, NY 10013, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Powers
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA; New York Genome Center, New York, NY 10013, USA.
authors.forename: Rahul
authors.initials: R
authors.lastname: Satija
authors.affiliation: Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA. Electronic address: ken.birnbaum@nyu.edu.
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Birnbaum
mesh_terms: D003583:Cytokinins / Q000378:metabolism; D020869:Gene Expression Profiling; D007210:Indoleacetic Acids / Q000378:metabolism; D059828:Plant Cells; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000502:physiology*; D012639:Seeds; D059010:Single-Cell Analysis; D055153:Stem Cell Niche; D013234:Stem Cells / Q000166:cytology
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators