title: In vivo Real-Time Mass Spectrometry for Guided Surgery Application.
abstract: Here we describe a new instrument (SpiderMass) designed for in vivo and real-time analysis. In this instrument ion production is performed remotely from the MS instrument and the generated ions are transported in real-time to the MS analyzer. Ion production is promoted by Resonant Infrared Laser Ablation (RIR-LA) based on the highly effective excitation of O-H bonds in water molecules naturally present in most biological samples. The retrieved molecular patterns are specific to the cell phenotypes and benign versus cancer regions of patient biopsies can be easily differentiated. We also demonstrate by analysis of human skin that SpiderMass can be used under in vivo conditions with minimal damage and pain. Furthermore SpiderMass can also be used for real-time drug metabolism and pharmacokinetic (DMPK) analysis or food safety topics. SpiderMass is thus the first MS based system designed for in vivo real-time analysis under minimally invasive conditions.
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.|Univ. Lille, CNRS, UMR 8523 - PhLAM - Physique des Lasers Atomes et Molécules, F-59000 Lille, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Fatou
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Saudemont
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.|Department of Gynecology Oncology, Cancer Center Oscar Lambret, Lille, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Leblanc
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.|Département Universitaire de Gynécologie Obstétrique, Service de chirurgie gynécologique, Hôpital Jeanne de Flandre, CHRU de Lille, 59037 Lille Cedex, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Vinatier
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.|Département Universitaire de Gynécologie Obstétrique, Service de chirurgie gynécologique, Hôpital Jeanne de Flandre, CHRU de Lille, 59037 Lille Cedex, France.
authors.forename: Violette
authors.initials: V
authors.lastname: Mesdag
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.
authors.forename: Maxence
authors.initials: M
authors.lastname: Wisztorski
authors.affiliation: Univ. Lille, CNRS, UMR 8523 - PhLAM - Physique des Lasers Atomes et Molécules, F-59000 Lille, France.
authors.forename: Cristian
authors.initials: C
authors.lastname: Focsa
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Salzet
authors.affiliation: Univ. Lille, CNRS, UMR 8523 - PhLAM - Physique des Lasers Atomes et Molécules, F-59000 Lille, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Ziskind
authors.affiliation: Univ. Lille, INSERM, U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fournier
mesh_terms: D005260:Female; D006801:Humans; D053685:Laser Therapy / Q000295:instrumentation; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation*; D019060:Minimally Invasive Surgical Procedures / Q000295:instrumentation*; D012867:Skin / Q000473:pathology