title: Authentication of Gentiana straminea Maxim. and its substitutes based on chemical profiling of iridoids using liquid chromatography with mass spectrometry.
abstract: A liquid chromatography-electrospray ionization-ion-trap mass spectrometry (MSn) method was established and applied for authentication of Gentiana straminea from the four substitutes, G. tibetica, G. lhassica, G. waltonii and G. robusta, based on chemical profiling of the principal iridoid glucosides aided by a quadrupole time-of-flight mass spectrometry. The fragmentation pathways of the three representative iridoid glucosides, loganic acid, gentiopicroside and sweroside, were investigated by MSnanalysis in negative ion mode, which assisted the characterization of analogs detected in the chromatographic profiling of the tested Gentiana species. In total, 25 iridoids were identified or tentatively characterized from G. straminea and four substitutes, in which 7-O-(4''-O-glucosyl)coumaroyl-loganic acid and 7-O-coumaroyl-loganic acid are diagnostic in G. straminea and can serve as the proposed chemical markers to discriminate it from morphologically similar substitutes.
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Wu
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D030012:Gentiana / Q000737:chemistry*; D039823:Iridoids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D039823:Iridoids
keywords: Gentiana straminea; Gentianae Macrophyllae Radix; LC -MSn; fingerprint; iridoid glucosides