title: Isolation and identification of anti-proliferative peptides from Spirulina platensis using three-step hydrolysis.
abstract: BACKGROUND
  Spirulina platensis is an excellent source of proteins (>60%) that can be hydrolyzed into bioactive peptides.
  
  
  RESULTS
  In this study, whole proteins of Spirulina platensis were extracted and hydrolyzed using three gastrointestinal endopeptidases (pepsin, trypsin and chymotrypsin). Subsequently, gel filtration chromatography was employed to separate hydrolysates, and four fractions (Tr1-Tr4) were obtained. Among them, Tr2 showed the strongest anti-proliferation activities on three cancer cells (MCF-7, HepG-2 and SGC-7901), with IC50values of <31.25, 36.42 and 48.25 µg mL-1, respectively. Furthermore, a new peptide, HVLSRAPR, was identified from fraction Tr1. This peptide exhibited strong inhibition on HT-29 cancer cells with an IC50value of 99.88 µg mL-1.
  
  
  CONCLUSIONS
  Taken together, these peptides possessed anti-proliferation activities on cancer cells and low cytotoxicity on normal cells, suggesting that they might serve as a natural anticancer agent for nutraceutical and pharmaceutical industries. © 2016 Society of Chemical Industry.
authors.affiliation: College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D020418:Algal Proteins / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000595:Amino Acid Sequence; D016588:Anticarcinogenic Agents / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002681:China; D002918:Chymotrypsin / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D019587:Dietary Supplements / Q000009:adverse effects; D055808:Drug Discovery; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009842:Oligopeptides / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000737:chemistry; D053545:Spirulina / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D016588:Anticarcinogenic Agents; D001426:Bacterial Proteins; C000620291:His-Val-Leu-Ser-Arg-Ala-Pro-Arg; D009842:Oligopeptides; D010446:Peptide Fragments; D011492:Protein Hydrolysates; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A
keywords: Spirulina platensis; anti-proliferation; gastrointestinal endopeptidases; hydrolysis