title: Facile One-Step Strategy for Highly Boosted Microbial Extracellular Electron Transfer of the Genus Shewanella.
abstract: High performance of bacterial extracellular electron transfer (EET) is essentially important for its practical applications in versatile bioelectric fields. We developed a facile one-step approach to dramatically boost the bacterial EET activity 75-fold by exogenous addition of ethylenediamine tetraacetic acid disodium salt (EDTA-2Na, 1 mM) into the electrochemical cells, where the anodic process of microbial EET was monitored. We propose that EDTA-2Na enables both the alternation of the local environment around the c-type cytochromes located on the outer membrane channels (OMCs), which therefore changes the redox behavior of OMCs in mediating the EET process, and the formation of densely packed biofilm that can further facilitate the EET process. As a synergistic effect, the highly boosted bacterial EET activity was achieved. The method shows good generality for versatile bioelectrical bacteria. We envision that the method is also applicable for constructing various bioelectric devices.
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, and ‡International Research Institute for Multidisciplinary Science, Beihang University , Beijing 100191, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, and ‡International Research Institute for Multidisciplinary Science, Beihang University , Beijing 100191, People's Republic of China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Lv
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, and ‡International Research Institute for Multidisciplinary Science, Beihang University , Beijing 100191, People's Republic of China.
authors.forename: Qingan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, and ‡International Research Institute for Multidisciplinary Science, Beihang University , Beijing 100191, People's Republic of China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ding
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, and ‡International Research Institute for Multidisciplinary Science, Beihang University , Beijing 100191, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, and ‡International Research Institute for Multidisciplinary Science, Beihang University , Beijing 100191, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
mesh_terms: D001674:Bioelectric Energy Sources; D004566:Electrodes; D004579:Electron Transport; D004583:Electrons; D010084:Oxidation-Reduction; D020592:Shewanella
keywords: cytochromes; electrochemistry; electron transfer; local environment; microbes