title: Functional Reconstruction of NLRs in HEK293 Cells.
abstract: Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.
authors.affiliation: Institut de Génomique Fonctionnelle, Labex ICST, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Université Montpellier, Montpellier, France. Vincent.compan@igf.cnrs.fr.|Institut National de la Santé et de la Recherche Médicale Unité 1191, Montpellier, France. Vincent.compan@igf.cnrs.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Compan
authors.affiliation: Manchester Collaborative Centre of Inflammation Research, Faculty of Life Sciences, The University of Manchester, Manchester, M13 9NT, UK.
authors.forename: Gloria
authors.initials: G
authors.lastname: López-Castejón
mesh_terms: D020170:Caspase 1 / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000378:metabolism*; D009550:Nigericin / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D020170:Caspase 1; D009550:Nigericin
keywords: ASC; Active caspase-1; HEK293; Inflammasome; NLR