title: A Multivariate Approach to Evaluate Biomass Production, Biochemical Composition and Stress Compounds of Spirulina platensis Cultivated in Wastewater.
abstract: The study was performed to investigate the effects of using cow effluent for the cultivation of Spirulina platensis on its biomass production and cell physiology. S. platensis was cultivated in three different cow effluents (CE) used as cultivation medium during 15 days. CE was prepared using dry cow manures, and it was further modified with supplement of NaNO3(CEN) and NaNO3+ NaCl (CENS). High nitrate value stimulated chlorophyll-a and total protein content of the cyanobacterium and also biomass production in standards medium (SM) and CEN media. Total carbohydrate content of S. platensis grown in CE media was found to be higher (p < 0.05) than that of SM. Productions of biomass and biochemical compounds by the cyanobacterium grown on the CE and SM media were evaluated by using multivariate approach. Conductivity, oxidation reduction potential (ORP), salinity, pH, and TDS played important role (p < 0.01) in the biochemical composition. As an effective explanatory factor, ORP had a significant positive correlation with H2O2, whereas negatively correlated with chlorophyll-α, biomass production, filament length, and proline. Canonical correspondence analysis proposed that biochemical compounds of S. platensis were not only affected by salinity and nutrition of media but also by pH and ORP. The present study indicated that CEN as a low cost model medium had high potential for the production of biomass by S. platensis with high protein content.
authors.affiliation: Department of Biology, Faculty of Art and Science, Gaziantep University, 27310, Gaziantep, Turkey. celekli.a@gmail.com.
authors.forename: Abuzer
authors.initials: A
authors.lastname: Çelekli
authors.affiliation: Department of Biology, Faculty of Art and Science, Gaziantep University, 27310, Gaziantep, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Topyürek
authors.affiliation: Department of Natural Resources Management and Agricultural Engineering, Agricultural University of Athens, 11855, Athens, Greece.
authors.forename: Giorgos
authors.initials: G
authors.lastname: Markou
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Gaziantep University, 27310, Gaziantep, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Bozkurt
mesh_terms: D000818:Animals; D018533:Biomass; D002417:Cattle; D009010:Monte Carlo Method; D015999:Multivariate Analysis; D017550:Spectroscopy, Fourier Transform Infrared; D053545:Spirulina / Q000254:growth & development* / Q000378:metabolism*; D013312:Stress, Physiological; D062065:Wastewater
chemical_list: D062065:Wastewater
keywords: Biochemical compound; Biomass; Cow effluent; Spirulina platensis