title: A High-resolution Typing Assay for Uropathogenic Escherichia coli Based on Fimbrial Diversity.
abstract: Urinary tract infections (UTIs) are one of the most common bacterial infections in humans, causing cystitis, pyelonephritis, and renal failure. Uropathogenic Escherichia coli (UPEC) is the leading cause of UTIs. Accurate and rapid discrimination of UPEC lineages is useful for epidemiological surveillance. Fimbriae are necessary for the adherence of UPEC strains to host uroepithelia, and seem to be abundant and diverse in UPEC strains. By analyzing all the possible fimbrial operons in UPEC strains, we found that closely related strains had similar types of chaperone-usher fimbriae, and the diversity of fimbrial genes was higher than that of multilocus sequence typing (MLST) genes. A typing assay based on the polymorphism of four gene sequences (three fimbrial genes and one housekeeping gene) and the diversity of fimbriae present was developed. By comparison with the MLST, whole-genome sequence (WGS) and fumC/fimH typing methods, this was shown to be accurate and have high resolution, and it was also relatively inexpensive and easy to perform. The assay can supply more discriminatory information for UPEC lineages, and have the potential to be applied in epidemiological surveillance of UPEC isolates.
authors.affiliation: Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical UniversityTianjin, China; Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd.Shanghai, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Department of General Microbiology, Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz Lodz, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Palusiak
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education - Tianjin Key Laboratory of Microbial Functional Genomics, TEDA Institute of Biological Sciences and Biotechnology, Nankai University Tianjin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical University Tianjin, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical University Tianjin, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical University Tianjin, China.
authors.forename: Huiting
authors.initials: H
authors.lastname: Wei
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University Chengdu, China.
authors.forename: Qingke
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Department of General Microbiology, Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz Lodz, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Rozalski
authors.affiliation: Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical University Tianjin, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical UniversityTianjin, China; State Key Laboratory of Medicinal Chemical Biology, NanKai UniversityTianjin, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
keywords: Uropathogenic Escherichia coli; epidemiological surveillance; fimbriae; lineages; typing