title: Genome scan linkage analysis identifies quantitative trait loci affecting serum clinical-chemical traits in Korean native chicken.
abstract: Alterations in robustness- and health-related traits lead to physiological changes, such as changes in the serum clinical chemical parameters in individuals. Therefore, clinical-chemical traits can be used as biomarkers to examine the health status of chickens. The aim of the present study was to detect the quantitative trait loci (QTLs) influencing eight clinical-chemical traits (glucose, total protein, creatinine, high-density lipoprotein cholesterol, total cholesterol, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, and α-amylase) in an F1 nuclear families comprising 83 F0 founders and 585 F1 progeny of Korean native chickens. Genotypic data on 135 DNA markers representing 26 autosomes have been generated for this resource pedigree. The total length of the map was 2729.4 cM. We used a multipoint variance component linkage approach to identify QTLs for the traits. A significant QTL affecting serum α-amylase levels was identified on chicken chromosome (GGA) 7 [logarithm of odds (LOD) = 3.02, P value = 1.92 × 10(-4)]. Additionally, we detected several suggestive linkage signals for the levels of total cholesterol, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, and creatinine on GGA 4, 12, 13, and 15. In this study, serum α-amylase levels related significant QTL was mapped on GGA7 and cholesterol, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, and creatinine traits related suggestive QTLs were detected on GGA4, 12, 13 and 15, respectively. Further verification and fine mapping of these identified QTLs can provide valuable information for understanding the variations of clinical chemical trait in chickens.
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Korea.
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Korea.
authors.forename: Hee-Bok
authors.initials: HB
authors.lastname: Park
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Korea.
authors.forename: Shil
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Korea.|Department of Animal Science, Sebelas Maret University, Surakarta, 57126, Indonesia.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Cahyadi
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Korea.
authors.forename: Nuri
authors.initials: N
authors.lastname: Choi
authors.affiliation: Poultry Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 31000, Korea.
authors.forename: Kang-Nyeong
authors.initials: KN
authors.lastname: Heo
authors.affiliation: Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute for Agriculture and Life Science, Seoul National University, Seoul, 08826, Korea.
authors.forename: Cheorun
authors.initials: C
authors.lastname: Jo
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Korea. junheon@cnu.ac.kr.
authors.forename: Jun-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D030161:Avian Proteins / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose; D001798:Blood Proteins / Q000378:metabolism; D002645:Chickens / Q000097:blood / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D005260:Female; D005819:Genetic Markers; D016678:Genome; D008075:Lipoproteins, HDL / Q000097:blood; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D056910:Republic of Korea; D000516:alpha-Amylases / Q000097:blood
chemical_list: D030161:Avian Proteins; D015415:Biomarkers; D001786:Blood Glucose; D001798:Blood Proteins; D005819:Genetic Markers; D008075:Lipoproteins, HDL; D002784:Cholesterol; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000516:alpha-Amylases
keywords: Clinical–chemical trait; Korean native chicken; Microsatellite marker; Quantitative trait loci