title: Short-term ubiquinol supplementation reduces oxidative stress associated with strenuous exercise in healthy adults: A randomized trial.
abstract: Studies about Coenzyme Q10(CoQ10) supplementation on strenuous exercise are scarce, especially those related with oxidative stress associated with physical activity and virtually nonexistent with the reduced form, Ubiquinol. The objective of this study was to determine, for the first time, whether a short-term supplementation with Ubiquinol can prevent oxidative stress associated to strenuous exercise. The participants (n = 100 healthy and well trained, but not on an elite level) were classified in two groups: Ubiquinol (experimental group), and placebo group (control). The protocol consisted of conducting two identical strenuous exercise tests with a rest period between tests of 24 h. Blood and urine samples were collected from the participants before supplementation (basal value) (T1), after supplementation (2 weeks) (T2), after first physical exercise test (T3), after 24 h of rest (T4), and after second physical exercise test (T5).The increase observed in the lactate, isoprostanes, DNA damage, and hydroperoxide levels reveals the severity of the oxidative damage induced by the exercise. There was a reduction in the isoprostanes, 8-OHdG, oxidized LDL, and hydroperoxydes in the supplemented Ubiquinol group, an increase in total antioxidant status, fat soluble antioxidant (both plasma and membrane), and CAT activity. Also, NO in the Ubiquinol-supplemented group was maintained within a narrow range. Oxidative stress induced by strenuous exercise is accumulative and increases transiently in subsequent sessions of physical activity. A short-term supplementation (2 weeks) with Ubiquinol (200 mg/day) before strenuous exercise, decreases oxidative stress and increases plasma NO, fact that could improve endothelial function, energetic substrate supply, and muscle recovery after strenuous exercise. © 2016 BioFactors, 42(6):612-622, 2016.
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix," University of Granada, Granada, Spain.|Human Nutrition Ph.D. Program, University of Granada, Granada, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Sarmiento
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix," University of Granada, Granada, Spain.|Department of Physiology, University of Granada, Granada, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Diaz-Castro
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix," University of Granada, Granada, Spain.|Department of Biochemistry and Molecular Biology II, University of Granada, Granada, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Pulido-Moran
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix," University of Granada, Granada, Spain.|Department of Physiology, University of Granada, Granada, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Moreno-Fernandez
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix," University of Granada, Granada, Spain.|Department of Physiology, University of Granada, Granada, Spain.
authors.forename: Naroa
authors.initials: N
authors.lastname: Kajarabille
authors.affiliation: Departament of Physical Education, University of Granada, Granada, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Chirosa
authors.affiliation: Faculty of Health Sciences, University of Granada, Granada, Spain.
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Guisado
authors.affiliation: Departament of Physical Education, University of Granada, Granada, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Javier Chirosa
authors.affiliation: Faculty of Health Sciences, University of Granada, Granada, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Guisado
authors.affiliation: Institute of Nutrition and Food Technology "José Mataix," University of Granada, Granada, Spain.|Department of Physiology, University of Granada, Granada, Spain.
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Ochoa
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage*; D019587:Dietary Supplements; D004334:Drug Administration Schedule; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D005082:Physical Exertion / Q000187:drug effects*; D055070:Resistance Training; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives*
chemical_list: D000975:Antioxidants; D014451:Ubiquinone; C003741:ubiquinol
keywords: Ubiquinol; high intensity (strenuous) exercise; oxidative damage