title: Biomimetic proteolipid vesicles for targeting inflamed tissues.
abstract: A multitude of micro- and nanoparticles have been developed to improve the delivery of systemically administered pharmaceuticals, which are subject to a number of biological barriers that limit their optimal biodistribution. Bioinspired drug-delivery carriers formulated by bottom-up or top-down strategies have emerged as an alternative approach to evade the mononuclear phagocytic system and facilitate transport across the endothelial vessel wall. Here, we describe a method that leverages the advantages of bottom-up and top-down strategies to incorporate proteins derived from the leukocyte plasma membrane into lipid nanoparticles. The resulting proteolipid vesicles-which we refer to as leukosomes-retained the versatility and physicochemical properties typical of liposomal formulations, preferentially targeted inflamed vasculature, enabled the selective and effective delivery of dexamethasone to inflamed tissues, and reduced phlogosis in a localized model of inflammation.
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: R
authors.initials: R
authors.lastname: Molinaro
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.|CEINGE-Biotecnologie Avanzate s.c.a.r.l., Via G. Salvatore 486, 80145 Naples, Italy.|IRCCS SDN, Via Gianturco 113, 80143 Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Corbo
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: J O
authors.initials: JO
authors.lastname: Martinez
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.|Pain Therapy Service, Fondazione IRCCS Policlinico San Matteo, Pavia 27100, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Taraballi
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: M
authors.initials: M
authors.lastname: Evangelopoulos
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: S
authors.initials: S
authors.lastname: Minardi
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: I K
authors.identifier: 0000-0002-0020-6960
authors.initials: IK
authors.lastname: Yazdi
authors.affiliation: Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: P
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: E
authors.initials: E
authors.lastname: De Rosa
authors.affiliation: Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas 77555, USA.
authors.forename: M B
authors.initials: MB
authors.lastname: Sherman
authors.affiliation: Osteoncology and Rare Tumors Center, IRCCS Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST), 47014 Meldola, Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Vita
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: N E
authors.initials: NE
authors.lastname: Toledano Furman
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.|IRCCS SDN, Via Gianturco 113, 80143 Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Parodi
authors.affiliation: Department of Regenerative Medicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.|Department of Orthopedics, Houston Methodist Hospital, Houston, Texas 77030, USA.
authors.forename: E
authors.initials: E
authors.lastname: Tasciotti
mesh_terms: D040761:Biomimetic Materials / Q000378:metabolism*; D004337:Drug Carriers / Q000378:metabolism*; D007249:Inflammation / Q000473:pathology; D007962:Leukocytes / Q000166:cytology; D008565:Membrane Proteins / Q000378:metabolism; D011510:Proteolipids / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D008565:Membrane Proteins; D011510:Proteolipids