title: Vascular Cell Induction Culture System Using Arabidopsis Leaves (VISUAL) Reveals the Sequential Differentiation of Sieve Element-Like Cells.
abstract: Cell differentiation is a complex process involving multiple steps, from initial cell fate specification to final differentiation. Procambial/cambial cells, which act as vascular stem cells, differentiate into both xylem and phloem cells during vascular development. Recent studies have identified regulatory cascades for xylem differentiation. However, the molecular mechanism underlying phloem differentiation is largely unexplored due to technical challenges. Here, we established an ectopic induction system for phloem differentiation named Vascular Cell Induction Culture System Using Arabidopsis Leaves (VISUAL). Our results verified similarities between VISUAL-induced Arabidopsis thaliana phloem cells and in vivo sieve elements. We performed network analysis using transcriptome data with VISUAL to dissect the processes underlying phloem differentiation, eventually identifying a factor involved in the regulation of the master transcription factor gene APL Thus, our culture system opens up new avenues not only for genetic studies of phloem differentiation, but also for future investigations of multidirectional differentiation from vascular stem cells.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan p@bs.s.u-tokyo.ac.jp fukuda@bs.s.u-tokyo.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kondo
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Alif Meem
authors.initials: AM
authors.lastname: Nurani
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Saito
authors.affiliation: RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama City, Kanagawa, Yokohama 230-0045, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ichihashi
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Saito
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yamazaki
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba, Ibaraki, 305-8566, Japan Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba, Ibaraki, 305-8566, Japan Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohme-Takagi
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan p@bs.s.u-tokyo.ac.jp fukuda@bs.s.u-tokyo.ac.jp.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D052585:Phloem / Q000166:cytology / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology* / Q000378:metabolism*; D052584:Xylem / Q000166:cytology / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins