title: Genetic and Hematologic Endpoints in Astyanax altiparanae (Characidae) After Exposure and Recovery to Water-Soluble Fraction of Gasoline (WSFG).
abstract: The sublethal effects of water-soluble fraction of gasoline (WSFG, 1.5 % v/v) were evaluated in the freshwater fish, Astynax altiparanae, after acute exposure (96 h) under a semi-static system. In addition, the recovery process was assessed in the fish following contaminant depuration. Recovery treatments were carried out with gradual depuration (GD), consisting of 7 days in the WSFG, followed by 8 days in clean water; and treatments with total depuration in clean water for 15 (DEP 15) and 30 days (DEP 30). The effects were evaluated through the piscine micronucleus test and by differential counting of organic defense cells. Acute exposure increased the frequency of neutrophils. In the GD treatment, the thrombocyte count and erythrocytic nuclear abnormalities (ENA) increased. In the DEP 15 treatment, there was a reduction of ENA; and following 30 days of depuration (i.e., DEP 30), the number of lymphocytes increased and the thrombocyte count remained high. These results indicate a long-term response to a condition of stress from WSFG.
authors.affiliation: Postgraduate Program in Ecology and Conservation, Federal University of Parana, Curitiba, PR, Brazil. gabrielibiologia@gmail.com.
authors.forename: Gabrieli L
authors.initials: GL
authors.lastname: Galvan
authors.affiliation: Department of Genetics, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Juliana R
authors.initials: JR
authors.lastname: Lirola
authors.affiliation: Department of Genetics, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Karoline
authors.initials: K
authors.lastname: Felisbino
authors.affiliation: Department of Genetics, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Taynah
authors.initials: T
authors.lastname: Vicari
authors.affiliation: Department of Chemical Engineering, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Carlos I
authors.initials: CI
authors.lastname: Yamamoto
authors.affiliation: Department of Genetics, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Marta M
authors.initials: MM
authors.lastname: Cestari
mesh_terms: D000818:Animals; D060126:Characidae / Q000378:metabolism*; D005618:Fresh Water; D005742:Gasoline / Q000633:toxicity*; D015162:Micronucleus Tests; D014867:Water / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D005742:Gasoline; D014874:Water Pollutants, Chemical; D014867:Water
keywords: Ecotoxicology; Fish; Hydrocarbons; Micronucleus test