title: Enterovirus 71 induces anti-viral stress granule-like structures in RD cells.
abstract: Stress granules (SGs) are dynamic cytoplasmic granules formed in response to a variety of stresses, including viral infection. Several viruses can modulate the formation of SG with different effects, but the relationship between SG formation and EV71 infection is poorly understood. In this study, we report that EV71 inhibits canonical SGs formation in infected cells and induces the formation of novel RNA granules that were distinguished from canonical SGs in composition and morphology, which we termed 'SG like structures'. Our results also demonstrated that EV71 triggered formation of SG-like structures is dependent on PKR and eIF2α phosphorylation and requires ongoing cellular mRNA synthesis. Finally, we found that SG-like structures are antiviral RNA granules that promote cellular apoptosis and suppress EV71 propagation. Taken together, our findings explain the formation mechanism of SG-like structures induced by EV71 and shed light on virus-host interaction and molecular mechanism underlying EV71 pathogenesis.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China.
authors.forename: Yuanmei
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. Electronic address: timjszzd@163.com.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D003594:Cytoplasmic Granules / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D029821:Enterovirus A, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014779:Virus Replication; D019892:eIF-2 Kinase / Q000378:metabolism
chemical_list: D015852:Eukaryotic Initiation Factor-2; D012333:RNA, Messenger; D019892:eIF-2 Kinase
keywords: EV71; SG-like structures; Stress granules