title: Lipid accumulation from pinewood pyrolysates by Rhodosporidium diobovatum and Chlorella vulgaris for biodiesel production.
abstract: This study evaluated the suitability of pinewood pyrolysates as a carbon source for lipid production and cultivation of the oleaginous yeast Rhodosporidium diobovatum and the microalgae Chlorella vulgaris. Thermal decomposition of pinewood and fractional condensation were used to obtain an oil rich in levoglucosan which was upgraded to glucose by acid hydrolysis. Blending of pyrolytic sugars with pure glucose in both nitrogen rich and nitrogen limited conditions was studied for R. diobovatum, and under nitrogen limited conditions for C. vulgaris. Glucose consumption rate decreased with increasing proportions of pyrolytic sugars increasing cultivation time. While R. diobovatum was capable of growth in 100% (v/v) pyrolytic sugars, C. vulgaris growth declined rapidly in blends greater than 20% (v/v) until no growth was detected in blends >40%. Finally, the effects of pyrolysis sugars on lipid composition was evaluated and biodiesel fuel properties were estimated based on the lipid profiles.
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, Ontario N6A 3K7, Canada.
authors.forename: Luis
authors.initials: L
authors.lastname: Luque
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, Ontario N6A 3K7, Canada.
authors.forename: Valerie C A
authors.initials: VCA
authors.lastname: Orr
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, Ontario N6A 3K7, Canada.
authors.forename: Sean
authors.initials: S
authors.lastname: Chen
authors.affiliation: Sustainable Process Technology, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, Netherlands.
authors.forename: Roel
authors.initials: R
authors.lastname: Westerhof
authors.affiliation: Sustainable Process Technology, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, Netherlands.
authors.forename: Stijn
authors.initials: S
authors.lastname: Oudenhoven
authors.affiliation: Shell Global Solutions International, United States.
authors.forename: Guus van
authors.initials: GV
authors.lastname: Rossum
authors.affiliation: Sustainable Process Technology, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, Netherlands.
authors.forename: Sascha
authors.initials: S
authors.lastname: Kersten
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, Ontario N6A 3K7, Canada.
authors.forename: Franco
authors.initials: F
authors.lastname: Berruti
authors.affiliation: Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, Ontario N6A 3K7, Canada; RWTH Aachen, AVT - Biochemical Engineering, Worringerweg 1, 52074 Aachen, Germany. Electronic address: lrehmann@uwo.ca.
authors.forename: Lars
authors.initials: L
authors.lastname: Rehmann
mesh_terms: D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D056804:Biofuels; D048408:Chlorella vulgaris / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D028223:Pinus / Q000737:chemistry; D014934:Wood / Q000737:chemistry
chemical_list: D056804:Biofuels; D008055:Lipids; C014989:1,6-anhydro-beta-glucopyranose; D005947:Glucose; D009584:Nitrogen
keywords: Biodiesel; Chlorella vulgaris; Oleaginous microbes; Pyrolytic hydrolysates; Rhodosporidium