title: Molecular recognition between insulin and dextran encapsulated gold nanoparticles.
abstract: Insulin is a peptide hormone that can regulate the metabolism of carbohydrates and lipids. This hormone is closely related to glucose-uptake in cells and can control blood glucose levels. Dextran is a polysaccharide composed of glucose units. In this study, we discovered that dextran-encapsulated gold nanoparticles (AuNPs@Dextran) and nanoclusters (AuNCs@Dextran) can be used to recognize insulin. The dissociation constant of insulin toward AuNPs@Dextran was estimated to be ∼5.3 × 10-6M. The binding site on insulin toward the dextran on the nanoprobes was explored as well. It was found that the sequence of numbers 1-22 on the insulin B chain can interact with the dextran encapsulated nanoprobes. Additionally, we also demonstrated that the dextran-encapsulated nanoprobes could be used as concentration probes to selectively enrich trace amounts of insulin (∼1 pM) from serum samples. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 300, Taiwan.
authors.forename: Kai-Chieh
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 300, Taiwan.
authors.forename: Hsiang-Ling
authors.initials: HL
authors.lastname: Chiang
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 300, Taiwan.
authors.forename: Wei-Ru
authors.initials: WR
authors.lastname: Chiu
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 300, Taiwan. yuchie@mail.nctu.edu.tw.
authors.forename: Yu-Chie
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism*; D006046:Gold; D007328:Insulin / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D003911:Dextrans; D007328:Insulin; D006046:Gold
keywords: dextran; glucose; gold nanoclusters; gold nanoparticles; insulin