title: Retroreflective-type Janus microspheres as a novel contrast agent for enhanced optical coherence tomography.
abstract: Optical coherence tomography (OCT) is a well-developed technology that utilizes near-infrared light to reconstruct three-dimensional images of biological tissues with micrometer resolution. Improvements of the imaging contrast of the OCT technique are able to further widen its extensive biomedical applications. In this study, Janus microspheres were developed and used as a positive contrast agent for enhanced OCT imaging. Phantom and ex vivo liver tissue experiments as well as in vivo animal tests were conducted, which validated that Janus microspheres, as a novel type of OCT tracer, were very effective in improving the OCT imaging contrast. Working principle and SEM image of Janus microsphere (top). Enhanced OCT imaging (bottom) of Janus microspheres in zebrafish stomach (blue dash line) and sinusoids (green arrows) of nude liver.
authors.affiliation: Bioimaging Core, Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center for Drug Non-clinical Evaluation and Research, Guangdong Biological Resources Institute, Guangdong Academy of Sciences, Guangzhou, 510900, China.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Wang
authors.affiliation: Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Bioimaging Core, Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry*; D005260:Female; D021621:Imaging, Three-Dimensional; D008099:Liver / Q000000981:diagnostic imaging; D008297:Male; D008819:Mice, Nude; D008863:Microspheres; D019047:Phantoms, Imaging; D041623:Tomography, Optical Coherence; D015027:Zebrafish
chemical_list: D003287:Contrast Media
keywords: Janus microsphere; contrast enhancement; optical coherence tomography; retroreflective-type