title: A Comparison of Conventional Collagen Sponge and Collagen-Gelatin Sponge in Wound Healing.
abstract: The objective of this study was to compare the effectiveness of the collagen-gelatin sponge (CGS) with that of the collagen sponge (CS) in dermis-like tissue regeneration. CGS, which achieves the sustained release of basic fibroblast growth factor (bFGF), is a promising material in wound healing. In the present study, we evaluated and compared CGSs and conventional CSs. We prepared 8 mm full-thickness skin defects on the backs of rats. Either CGSs or CSs were impregnated with normal saline solution (NSS) or 7 μg/cm(2) of bFGF solution and implanted into the defects. At 1 and 2 weeks after implantation, tissue specimens were obtained from the rats of each group (n = 3, total n = 24). The wound area, neoepithelial length, dermis-like tissue area, and the number and area of capillaries were evaluated at 1 and 2 weeks after implantation. There were no significant differences in the CGS without bFGF and CS groups. Significant improvements were observed in the neoepithelial length, the dermis-like tissue area, and the number of newly formed capillaries in the group of rats that received CGSs impregnated with bFGF. The effects on epithelialization, granulation, and vascularization of wound healing demonstrated that, as a scaffold, CGSs are equal or superior to conventional CSs.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-machi, Syougoin, Sakyou-ku, Kyoto City, Kyoto 606-8507, Japan.
authors.forename: Chizuru
authors.initials: C
authors.lastname: Jinno
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata City, Osaka 573-1010, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Morimoto
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-machi, Syougoin, Sakyou-ku, Kyoto City, Kyoto 606-8507, Japan.
authors.forename: Ran
authors.initials: R
authors.lastname: Ito
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-machi, Syougoin, Sakyou-ku, Kyoto City, Kyoto 606-8507, Japan.
authors.forename: Michiharu
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-machi, Syougoin, Sakyou-ku, Kyoto City, Kyoto 606-8507, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Ogino
authors.affiliation: GUNZE Ltd. QOL Research Center Laboratory, 1 Ishiburo, Inokura Shinmachi, Ayabe, Kyoto 623-8512, Japan.
authors.forename: Tsuguyoshi
authors.initials: T
authors.lastname: Taira
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-machi, Syougoin, Sakyou-ku, Kyoto City, Kyoto 606-8507, Japan.
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D003094:Collagen / Q000627:therapeutic use*; D004847:Epithelial Cells / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D005780:Gelatin / Q000627:therapeutic use; D048091:Guided Tissue Regeneration; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000254:growth & development / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016222:Fibroblast Growth Factor 2; D005780:Gelatin; D003094:Collagen