title: Salivary and Serum Antibody Response Against Neisseria meningitidis After Vaccination With Conjugate Polysaccharide Vaccines in Ethiopian Volunteers.
abstract: Meningococcal conjugate vaccines induce serum antibodies crucial for protection against invasive disease. Salivary antibodies are believed to be important for hindering meningococcal acquisition and/or clearance of established carriage. In this study, we measured salivary IgA and IgG antibodies induced by vaccination with a monovalent serogroup A conjugate vaccine or a tetravalent A, C, W and Y conjugate vaccine, in comparison with antibody levels in serum. Saliva and serum samples from Ethiopian volunteers (1-29 years) collected before and eight times on a weekly basis after receiving the serogroup A conjugate vaccine, the tetravalent serogroup A, C, W and Y conjugate vaccine, or no vaccine (control group), were analysed using a multiplex microsphere immunoassay for antibody detection. Serogroup-specific IgG antibody levels in saliva increased significantly after vaccination with both vaccines. The monovalent serogroup A vaccine also induced an increase in salivary IgA antibodies. A strong correlation between serogroup-specific IgG antibodies in saliva and serum, and a somewhat lower correlation for IgA, was observed for all serogroups. There was also a strong correlation between specific secretory IgA and IgA antibodies in saliva for all serogroups. Meningococcal conjugate vaccines are able to elicit salivary antibodies against serogroup A, C, W and Y correlating with antibody levels in serum. The strong correlation between saliva and serum antibody levels indicates that saliva may be used as a surrogate of systemic antibody responses.
authors.affiliation: Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway.|Department of Community Medicine, Section of International Community Health, University of Oslo, Oslo, Norway.
authors.forename: G K
authors.initials: GK
authors.lastname: Bårnes
authors.affiliation: Arba Minch College of Health Sciences, Arba Minch, Ethiopia.
authors.forename: B
authors.initials: B
authors.lastname: Workalemahu
authors.affiliation: Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway.
authors.forename: P A
authors.initials: PA
authors.lastname: Kristiansen
authors.affiliation: Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
authors.forename: D
authors.initials: D
authors.lastname: Beyene
authors.affiliation: College of Medicine and Health Sciences, Arba Minch University, Arba Minch, Ethiopia.
authors.forename: B
authors.initials: B
authors.lastname: Merdekios
authors.affiliation: Arba Minch General Hospital, Arba Minch, Ethiopia.
authors.forename: P
authors.initials: P
authors.lastname: Fissiha
authors.affiliation: Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
authors.forename: A
authors.initials: A
authors.lastname: Aseffa
authors.affiliation: Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway.|Department of Community Medicine, Section of International Community Health, University of Oslo, Oslo, Norway.
authors.forename: D A
authors.initials: DA
authors.lastname: Caugant
authors.affiliation: Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway.
authors.forename: L M
authors.initials: LM
authors.lastname: Naess
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000378:metabolism*; D000917:Antibody Formation; D001798:Blood Proteins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005002:Ethiopia; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007223:Infant; D008297:Male; D008585:Meningitis, Meningococcal / Q000276:immunology / Q000517:prevention & control*; D009345:Neisseria meningitidis / Q000276:immunology*; D012463:Saliva / Q000378:metabolism*; D065288:Serogroup; D014611:Vaccination; D018074:Vaccines, Conjugate / Q000276:immunology*; D014838:Volunteers; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D001798:Blood Proteins; D007070:Immunoglobulin A; D018074:Vaccines, Conjugate