title: Dynamic high pressure microfluidization treatment of zein in aqueous ethanol solution.
abstract: Dynamic high pressure microfluidization (DHPM) treatment at the pressures of 25, 50, 75, 100, 125 and 150MPa was performed to explore its effects on the characteristics of zein in aqueous ethanol solution. The results showed that after DHPM treatment at 125MPa, the volume percentage of zein nanoparticles (d<100nm) was increased from 68.8% to 94.7%, and the fluorescence intensity approached to the maximum. DHPM treatment at the pressure of 25MPa led to the increase of α-helix and β-sheet of zein from 57.1% to 59.4% and from 16.8% to 17.9%, respectively. The partial denaturation of zein was induced after DHPM process at pressures from 50 to 150MPa. The morphology of zein was modified from nanosphere to the needle-like shapes after DHPM treatment at 75MPa, and the deduction was proposed that the morphological change at 75MPa was ascribed to the existence of the intermediate transition state.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, 100083, China.
authors.forename: Cuixia
authors.initials: C
authors.lastname: Sun
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, 100083, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dai
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, 100083, China.
authors.forename: Fuguo
authors.initials: F
authors.lastname: Liu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, 100083, China. Electronic address: gyxcau@126.com.
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000431:Ethanol / Q000737:chemistry*; D011312:Pressure; D015028:Zein / Q000737:chemistry*
chemical_list: D000431:Ethanol; D015028:Zein
keywords: DHPM treatment; Morphology; Structure; Thermal behaviors; Zein