title: MiR-31 Functions as a Tumor Suppressor in Lung Adenocarcinoma Mainly by Targeting HuR.
abstract: BACKGROUND
  Gene expression is widely regulated by miRNAs and RNA binding proteins. In this study, we mainly focused on miR-31 and a RNA binding protein, HuR (Hu antigen R).
  
  
  METHODS
  The levels of miR-31 and HuR in lung carcinoma cells and lung cancer tissues were explored using RT-qPCR and western blot, respectively. Luciferase reporter assay was used to determine the target gene of miR-31. Cell apoptosis and migration were studied using flow cytometry and the transwell invasion assay. The down-stream genes of HuR were explored with western blot assay.
  
  
  RESULTS
  miR-31 was decreased in lung carcinoma cells and lung cancer tissues, while the protein level of HuR was increased. HuR was the target gene of miR-31. Inhibition of miR-31 and overexpression of HuR resulted in the upregulation of cyclins A2, B1, D1 and VEGF (vascular endothelial growth factor). Furthermore, overexpression of miR-31 prompted lung cancer cell apoptosis and inhibited cell migration.
  
  
  CONCLUSIONS
  Reduction of miR-31 expression enhanced lung cancer proliferation and migration by repressing HuR expression.
authors.forename: Hai
authors.initials: H
authors.lastname: Xu
authors.forename: Jianqun
authors.initials: J
authors.lastname: Ma
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Changfa
authors.initials: C
authors.lastname: Qu
authors.forename: Fenglin
authors.initials: F
authors.lastname: Sun
authors.forename: Shidong
authors.initials: S
authors.lastname: Xu
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis; D049109:Cell Proliferation; D000067780:ELAV-Like Protein 1 / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness
chemical_list: D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; C534612:MIRN31 microRNA, human; D035683:MicroRNAs