title: [The Utility of XE-2 100 Analyzer's NEUT-X and NEUT-Y Parameters for Detecting Neutrophil Dysplasia in Myelodysplastic Syndromes].
abstract: Morphological abnormalities of blood cells are the typical characteristics of myelodysplastic syndromes (MDS). Recently, the usefulness of multiparameter automatic hematology analyzer XE-2100 for detecting neutrophil dysplasia has been reported by using parameters of NEUT-X and NEUT-Y, reflecting neutrophil cytoplasmic granularity and the cellular content of nucleic acid and protein, respectively. We evaluated the utility of these parameters by analyzing the blood samples of fifty MDS patients consulting Kakogawa West Municipal Hospital between Jan, 2010 and Jun, 2014, as well as 100 persons undergoing medical examinations during the same period as controls. Neutrophil granulation level was classified as normal, hypo-granular, or agranular under microscopic observation, and degranulation index was calculated using the following formula. The relationship between NEUT-X, NEUT-Y values and degranulation index was studied as below. Degranulation index = agranular (%) x 2+ hypo-granular (%) x 1 + normal (%) x 0 Neut-X values of MDS patients were 1,350 (mean), 1,345 (median), and NEUT-Y values, 430 (mean) and 432 (median). The NEUT-X and NEUT-Y values of control patients were 1,350, 1,349, 446 and 445, respectively. Correlation efficiency between degranulation index and NEUT-X or NEUT-Y were r = 0.62 or 0.52, respectively. Relationship between NEUT-X and NEUT-Y for all patients was r = 0.90. All the 10 patients showing NEUT-X lower than 1,315 and NEUT-Y lower than 400 simultaneously were MDS. Hence, we conclude that NEUT-X and NEUT-Y information is useful for quantitative evaluation of neutrophil morphological abnormalities.
authors.forename: Setsuki
authors.initials: S
authors.lastname: Isono
authors.forename: Atsuo
authors.initials: A
authors.lastname: Okamur
authors.forename: Mika
authors.initials: M
authors.lastname: Iwamoto
authors.lastname: ImaiJunko IMAI
authors.forename: Keiko
authors.initials: K
authors.lastname: Nagata
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Akishino
authors.forename: Akiharu
authors.initials: A
authors.lastname: Okamura
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Mari
authors.initials: M
authors.lastname: Kono
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sugimoto
authors.forename: Katsuyasu
authors.initials: K
authors.lastname: Saigo
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D009504:Neutrophils / Q000473:pathology*; D055815:Young Adult