title: Effects of short-term hypoxia and seawater acidification on hemocyte responses of the mussel Mytilus coruscus.
abstract: Hypoxia often intensifies with rising dissolved CO2, but the concurrent effects of hypoxia and acidification on bivalves are largely unknown. In this study, immune responses of hemocytes in the mussel Mytilus coruscus were examined under six combinations of pH (7.3, 7.7 and 8.1) and dissolved oxygen (DO) concentrations (2mgL(-1), 6mgL(-1)) for 72h. Generally, total hemocyte account, phagocytosis, esterase and lysosomal content were reduced under low DO and pH conditions, whereas hemocyte mortality and reactive oxygen species production increased under low DO and pH. Both hypoxia and low pH have negative effects on mussels, but the effects of pH are not as strong as DO. Moreover, significant interactions between DO and pH occurred. However, acidification generally doesn't aggravate the effects induced by hypoxia. Acidification and hypoxia may increase disease risk and impact the aquaculture of this species.
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China. Electronic address: 416597727@qq.com.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Sui
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Yueyong
authors.initials: Y
authors.lastname: Shang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Xizhi
authors.initials: X
authors.lastname: Huang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: FangLi
authors.initials: F
authors.lastname: Wu
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Department of Integrative Ecophysiology, Alfred-Wegener-Institute Helmholtz Center for Polar- and Marine Research, 27570 Bremerhaven, Germany.
authors.forename: Menghong
authors.initials: M
authors.lastname: Hu
authors.affiliation: Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China; Department of Environmental Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Daohui
authors.initials: D
authors.lastname: Lin
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: wqlv@shou.edu.cn.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Lu
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Department of Integrative Ecophysiology, Alfred-Wegener-Institute Helmholtz Center for Polar- and Marine Research, 27570 Bremerhaven, Germany; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China. Electronic address: youjiwang2@gmail.com.
authors.forename: Youji
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D006434:Hemocytes / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia; D049878:Mytilus / Q000187:drug effects*; D012623:Seawater / Q000737:chemistry*
keywords: Acidification; Flow cytometry; Hemocyte; Hypoxia; Immune response; Mytilus coruscus