title: Inactivation of Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7 on cantaloupes by octenidine dihydrochloride.
abstract: The efficacy of a new generation disinfectant, octenidine dihydrochloride (OH), as wash and coating treatments for reducing Listeria monocytogenes (LM), Salmonella spp. (SAL), and Escherichia coli O157:H7 (EC) on cantaloupe was investigated. Cantaloupe rind plugs inoculated separately with the three bacterial species (∼8 log CFU/cm(2)) were washed for 1, 3, 5 min at 25 °C in water, or chlorine (200 ppm), ethanol (1%), OH (0.01, 0.05, 0.1%) and surviving populations were measured after treatment. Additionally, inoculated cantaloupe rind plugs were coated with 2% chitosan or chitosan containing OH (0.01, 0.05, 0.1%) and sampled for surviving pathogens. Subsequently, the antimicrobial efficacy of OH wash and coating (0.1, 0.2%) on whole cantaloupes was determined. All OH wash reduced LM, SAL, and EC on cantaloupe rinds by > 5 log CFU/cm(2) by 2 min, and reduced populations to undetectable levels (below 2 log CFU/cm(2)) by 5 min (P < 0.05). Similarly, OH coating on cantaloupe rinds reduced the pathogens by 3-5 log /cm(2) (P < 0.05). Washing and coating whole cantaloupes with OH reduced the three pathogens by at least 5 log and 2 log CFU/cm(2), respectively (P < 0.05). Results suggest that OH could be used as antimicrobial wash and coating to reduce LM, SAL, and EC on cantaloupes.
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Abhinav
authors.initials: A
authors.lastname: Upadhyay
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Hsinbai
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Indu
authors.initials: I
authors.lastname: Upadhyaya
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Samantha
authors.initials: S
authors.lastname: Fancher
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Meera Surendran
authors.initials: MS
authors.lastname: Nair
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Leanne
authors.initials: L
authors.lastname: Jankelunas
authors.affiliation: Environmental Microbial & Food Safety Lab, USDA-ARS, 10300 Baltimore Avenue, Building 201 BARC-East, Room 101, Beltsville, MD 20705, USA.
authors.forename: Jitendra R
authors.initials: JR
authors.lastname: Patel
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA. Electronic address: kumar.venkitanarayanan@uconn.edu.
authors.forename: Kumar
authors.initials: K
authors.lastname: Venkitanarayanan
mesh_terms: D015169:Colony Count, Microbial; D028463:Cucumis melo / Q000382:microbiology*; D004202:Disinfectants / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D005516:Food Microbiology; D007097:Imines; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D011725:Pyridines / Q000494:pharmacology*; D012475:Salmonella / Q000187:drug effects* / Q000254:growth & development
chemical_list: D004202:Disinfectants; D007097:Imines; D011725:Pyridines; C034213:octenidine
keywords: Cantaloupes; Chitosan; Escherichia coli O157:H7; Listeria monocytogenes; Octenidine hydrochloride; Salmonella spp