title: Effect of CYP1A1 gene polymorphism and psychological distress on seminal analysis parameters.
abstract: BACKGROUND
  Psychological factor alters fertility hormones and contributes to male infertility. Anxiety and depression are common manifestations of psychological distress. Cytochrome P-4501A1 (CYP1A1) metabolizes xenobiotics and fertility hormones that influence male fertility. The effect of CYP1A1 polymorphism on male fertility has remained controversial. The present study was designed to assess the effect of psychological distress and CYP1A1 polymorphisms and their interactions on parameters of seminal analysis.
  
  
  METHODS
  Eighty male partners of infertile couples were evaluated for level of distress using Hospital anxiety and depression score (HADS) questionnaire. As per WHO guidelines (2010), sperm count, motility and morphology were assessed and subjects were classified as (a) subjects having normal sperm characteristics and (b) subjects having abnormal sperm characteristics. CYP1A1 polymorphisms were detected by ASO-PCR.
  
  
  RESULTS
  The significant odd's ratio indicates that psychological distress (OR:10.54; CI:3.72-29.84; P < 0.001), CYP1A1*4(OR:10.31; CI:3.01-35.24; P < 0.001) and CYP1A1*2C (OR:7.01; CI:1.78-27.56; P = 0.002) polymorphisms are risk factors for the development of abnormal sperm characteristics in male subjects. Data analysis with two way ANOVA shows that psychological distress, CYP1A1*4 and CYP1A1*2C polymorphisms significantly affect but do not interact among them to influence sperm parameters.
  
  
  CONCLUSIONS
  It is concluded that CYP1A1 gene polymorphisms and psychological distress act independently but do not interact with each other in pathogenesis of male infertility.
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
authors.forename: Aditi
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India. bckoner@hotmail.com.
authors.forename: Bidhan Chandra
authors.initials: BC
authors.lastname: Koner
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
authors.forename: Prakash Chandra
authors.initials: PC
authors.lastname: Ray
authors.affiliation: Department of Gynaecology and Obstetrics, IVF and Reproductive Biology Centre, Maulana Azad Medical College and Lok Nayak Hospital, New Delhi, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Prasad
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
authors.forename: Elvia
authors.initials: E
authors.lastname: Jamatia
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
authors.forename: Mirza
authors.initials: M
authors.lastname: Masroor
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Singh
mesh_terms: D000328:Adult; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007248:Infertility, Male / Q000209:etiology* / Q000235:genetics; D008297:Male; D011110:Polymorphism, Genetic; D011569:Psychiatric Status Rating Scales; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility / Q000235:genetics; D013094:Spermatozoa / Q000473:pathology; D013315:Stress, Psychological / Q000150:complications*; D055815:Young Adult
chemical_list: C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1
keywords: CYP1A1 polymorphism; Male infertility; Psychological distress; Sperm characteristics