title: Knockdown of COUP-TFII inhibits cell proliferation and induces apoptosis through upregulating BRCA1 in renal cell carcinoma cells.
abstract: COUP-TFII belongs to the nuclear receptor family, which is highly expressed in many kinds of tumors. Previous studies have shown that COUP-TFII can promote tumor progression through regulating tumor angiogenesis and cell proliferation and migration of certain cancer cells. However, the function of COUP-TFII in renal cell carcinoma (RCC) is not clear. Here, we showed that clinical RCC tumor tissues showed much higher COUP-TFII expression level than adjacent normal tissues. When COUP-TFII was knocked down in RCC 769-P and 786-O cells by siRNA or shRNA-expressing lentivirus, the cell proliferation was markedly inhibited, and apoptosis increased. Moreover, the tumor growth of COUP-TFII knockdown 769-P and 786-O xenografts in nude mice was also obviously inhibited. Using qRT-PCR and Western blot, we showed that the expression of the tumor suppressor gene BRCA1 was upregulated in COUP-TFII knockdown cells. Simultaneously knockdown of BRCA1 and COUP-TFII partially rescued the inhibited cell proliferation and increased apoptosis in COUP-TFII single knockdown cells. These results indicate that COUP-TFII may play an oncogenic role in RCC, and COUP-TFII may promote tumor progression through inhibiting BRCA1.
authors.affiliation: Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.|Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Dian
authors.initials: D
authors.lastname: Jiao
authors.affiliation: Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi'an, China.
authors.forename: Shengjia
authors.initials: S
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi'an, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Xi
authors.affiliation: Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi'an, China.
authors.forename: An-Gang
authors.initials: AG
authors.lastname: Yang
authors.affiliation: Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huan
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, Xi'an, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Wen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D019313:BRCA1 Protein / Q000096:biosynthesis / Q000235:genetics*; D051837:COUP Transcription Factor II / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D055785:Gene Knockdown Techniques; D019398:Genes, BRCA1; D064593:Heterografts; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D015854:Up-Regulation
chemical_list: D019313:BRCA1 Protein; D051837:COUP Transcription Factor II; D034741:RNA, Small Interfering; C492913:BRCA1 protein, human; C095569:NR2F2 protein, human
keywords: BRCA1; COUP-TFII; apoptosis; cell proliferation; renal cell carcinoma