title: Measuring NLR Oligomerization III: Detection of NLRP3 Complex by Bioluminescence Resonance Energy Transfer.
abstract: Bioluminescent resonance energy transfer (BRET) is a natural phenomenon resulting from a non-radiative energy transfer between a bioluminescent donor (Renilla luciferase) and a fluorescent protein acceptor. BRET signal is dependent on the distance and the orientation between the donor and the acceptor and could be used to study protein-protein interactions and conformational changes within proteins in real time in living cells. This protocol describes the use of BRET technique to study NLRP3 oligomerization in living cells before and during NLRP3 inflammasome activation.
authors.affiliation: Inflammation and Experimental Surgery Unit,, Institute for Bio-Health Research of Murcia (IMIB-Arrixaca), Carretera Buenavista, s/n., El Palmar, Murcia, 30120, Spain. fatima.martin@ffis.es.
authors.forename: Fátima
authors.initials: F
authors.lastname: Martín-Sánchez
authors.affiliation: Institut de Génomique Fonctionnelle, Labex ICST, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Université Montpellier, Montpellier, France.|Institut National de la Santé et de la Recherche Médicale Unité 1191, Montpellier, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Compan
authors.affiliation: Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Carretera Buenavista, s/n., Murcia, 30120, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pelegrín
mesh_terms: D060807:Bioluminescence Resonance Energy Transfer Techniques / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D058847:Inflammasomes / Q000737:chemistry; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000737:chemistry*; D011485:Protein Binding; D055503:Protein Multimerization
chemical_list: D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human
keywords: BRET; Bioluminescence; NLRP3; Sensor