title: Trace Level Determination of Mesityl Oxide and Diacetone Alcohol in Atazanavir Sulfate Drug Substance by a Gas Chromatography Method.
abstract: A capillary gas chromatography method with a short run time, using a flame ionization detector, has been developed for the quantitative determination of trace level analysis of mesityl oxide and diacetone alcohol in the atazanavir sulfate drug substance. The chromatographic method was achieved on a fused silica capillary column coated with 5% diphenyl and 95% dimethyl polysiloxane stationary phase (Rtx-5, 30 m x 0.53 mm x 5.0 µm). The run time was 20 min employing programmed temperature with a split mode (1:5) and was validated for specificity, sensitivity, precision, linearity, and accuracy. The detection and quantitation limits obtained for mesityl oxide and diacetone alcohol were 5 µg/g and 10 µg/g, respectively, for both of the analytes. The method was found to be linear in the range between 10 µg/g and 150 µg/g with a correlation coefficient greater than 0.999, and the average recoveries obtained in atazanavir sulfate were between 102.0% and 103.7%, respectively, for mesityl oxide and diacetone alcohol. The developed method was found to be robust and rugged. The detailed experimental results are discussed in this research paper.
authors.affiliation: Aurobindo Pharma Limited Research Centre-II, Survey No. 71&72, Indrakaran Village, Sangareddy Mandal, Medak-502329, Andhra Pradesh, India.
authors.forename: K V S N
authors.initials: KV
authors.lastname: Raju
authors.affiliation: Aurobindo Pharma Limited Research Centre-II, Survey No. 71&72, Indrakaran Village, Sangareddy Mandal, Medak-502329, Andhra Pradesh, India.
authors.forename: K S R
authors.initials: KS
authors.lastname: Pavan Kumar
authors.affiliation: Aurobindo Pharma Limited Research Centre-II, Survey No. 71&72, Indrakaran Village, Sangareddy Mandal, Medak-502329, Andhra Pradesh, India.
authors.forename: N
authors.initials: N
authors.lastname: Siva Krishna
authors.affiliation: Aurobindo Pharma Limited Research Centre-II, Survey No. 71&72, Indrakaran Village, Sangareddy Mandal, Medak-502329, Andhra Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Madhava Reddy
authors.affiliation: Aurobindo Pharma Limited Research Centre-II, Survey No. 71&72, Indrakaran Village, Sangareddy Mandal, Medak-502329, Andhra Pradesh, India.
authors.forename: N
authors.initials: N
authors.lastname: Sreenivas
authors.affiliation: Aurobindo Pharma Limited Research Centre-II, Survey No. 71&72, Indrakaran Village, Sangareddy Mandal, Medak-502329, Andhra Pradesh, India.
authors.forename: Hemant
authors.initials: H
authors.lastname: Kumar Sharma
authors.affiliation: Department of Engineering Chemistry, AU college of Engineering, Andhra University, Visakhapatnam-530003, Andhra Pradesh, India.
authors.forename: G
authors.initials: G
authors.lastname: Himabindu
authors.affiliation: Department of Engineering Chemistry, AU college of Engineering, Andhra University, Visakhapatnam-530003, Andhra Pradesh, India.
authors.forename: N
authors.initials: N
authors.lastname: Annapurna
keywords: Atazanavir sulfate; Development; Diacetone alcohol; GC-FID; Mesityl oxide; Validation