title: Investigation of the effect of α-melanocyte-stimulating hormone on proliferation and early stages of differentiation of human induced pluripotent stem cells.
abstract: We have studied the influence of α-melanocyte-stimulating hormone (α-MSH) on proliferation and early stages of differentiation of human induced pluripotent stem cells (iPSc). We have demonstrated that α-MSH receptor genes are expressed in undifferentiated iPSc. The expression levels of MCR1, MCR2, and MCR3 increased at the embryoid body (EB) formation stage. The formation of neural progenitors was accompanied by elevation of MCR2, MCR3, and MCR4 expression. α-MSH had no effect on EB generation and iPSc proliferation at concentrations ranging from 1 nM to 10 μM. At the same time, α-MSH increased the generation of neural rosettes in human iPSc cultures more than twice.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia. novek-img@mail.ru.
authors.forename: E V
authors.initials: EV
authors.lastname: Novosadova
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Manuilova
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Arsenyeva
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Andreeva
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: O S
authors.initials: OS
authors.lastname: Lebedeva
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Grivennikov
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: N F
authors.initials: NF
authors.lastname: Myasoedov
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D058953:Neural Stem Cells / Q000502:physiology; D009474:Neurons / Q000502:physiology; D011979:Receptors, Pituitary Hormone / Q000378:metabolism; D000521:alpha-MSH / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D011979:Receptors, Pituitary Hormone; D000521:alpha-MSH; C029446:MSH receptor