title: The apoptotic effect and the plausible mechanism of microwave radiation on rat myocardial cells.
abstract: Microwaves may exert adverse biological effects on the cardiovascular system at the integrated system and cellular levels. However, the mechanism underlying such effects remains poorly understood. Here, we report a previously uncharacterized mechanism through which microwaves damage myocardial cells. Rats were treated with 2450 MHz microwave radiation at 50, 100, 150, or 200 mW/cm(2) for 6 min. Microwave treatment significantly enhanced the levels of various enzymes in serum. In addition, it increased the malondialdehyde content while decreasing the levels of antioxidative stress enzymes, activities of enzyme complexes I-IV, and ATP in myocardial tissues. Notably, irradiated myocardial cells exhibited structural damage and underwent apoptosis. Furthermore, Western blot analysis revealed significant changes in expression levels of proteins involved in oxidative stress regulation and apoptotic signaling pathways, indicating that microwave irradiation could induce myocardial cell apoptosis by interfering with oxidative stress and cardiac energy metabolism. Our findings provide useful insights into the mechanism of microwave-induced damage to the cardiovascular system.
authors.affiliation: a Department of Biochemistry, Ji Lin Medical University, Ji Lin 132013, China.
authors.forename: Wenhe
authors.initials: W
authors.lastname: Zhu
authors.affiliation: b First Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: a Department of Biochemistry, Ji Lin Medical University, Ji Lin 132013, China.
authors.forename: Xianmin
authors.initials: X
authors.lastname: Feng
authors.affiliation: a Department of Biochemistry, Ji Lin Medical University, Ji Lin 132013, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Department of Biochemistry, Ji Lin Medical University, Ji Lin 132013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: a Department of Biochemistry, Ji Lin Medical University, Ji Lin 132013, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Xu
authors.affiliation: a Department of Biochemistry, Ji Lin Medical University, Ji Lin 132013, China.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Department of Biochemistry, Ji Lin Medical University, Ji Lin 132013, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Lv
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000528:radiation effects; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism / Q000528:radiation effects; D008872:Microwaves / Q000009:adverse effects*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects*; D018384:Oxidative Stress / Q000502:physiology / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde
keywords: cellule myocardique; energy metabolism; microwave radiation; myocardial cell; métabolisme énergétique; oxidative stress; rayonnement de micro-ondes; stress oxydatif