title: Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens.
abstract: RotaTeq(®) and Rotarix™ are two common human rotavirus (RV) vaccines currently on the market worldwide. Recent studies indicate histo-blood group antigens (HBGAs) may be attachment factors for RVs. The P[8] VP8* proteins of RotaTeq and Rotarix were expressed and purified, and their binding specificities were evaluated. Saliva-based binding assays showed that the VP8* proteins bound to the saliva samples of secretors irrespective of ABO blood types. However, in the oligosaccharide binding assay, the VP8* proteins displayed no specific binding to the HBGAs tested, including Lewis b and H1. The structure of RotaTeq P[8] VP8* was solved at 1.9Å. Structural comparisons revealed that the putative receptor binding site was different to that of other genotypes and displayed a novel potential binding region. These findings indicate RotaTeq and Rotarix may have better efficiency in areas with a high percentage of secretors.
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Sun
authors.affiliation: Clinical Laboratory of Hunan Provincial People's Hospital, The first Affiliated Hospital of Hunan Normal University, Changsha 410005, China.
authors.forename: Nijun
authors.initials: N
authors.lastname: Guo
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Dandi
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Guangcheng
authors.initials: G
authors.lastname: Xie
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Pang
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory of Hunan Provincial People's Hospital, The first Affiliated Hospital of Hunan Normal University, Changsha 410005, China. Electronic address: youde120@aliyun.com.
authors.forename: Youde
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of the People's Republic of China, Beijing 102206, China; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. Electronic address: zhaojund@126.com.
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000595:Amino Acid Sequence; D001789:Blood Group Antigens / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D009844:Oligosaccharides / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins; D012401:Rotavirus / Q000276:immunology / Q000378:metabolism; D022243:Rotavirus Vaccines / Q000276:immunology; D012463:Saliva / Q000276:immunology / Q000378:metabolism; D013329:Structure-Activity Relationship; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D001789:Blood Group Antigens; D009844:Oligosaccharides; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D022243:Rotavirus Vaccines; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus
keywords: Crystal structure; Histo-blood group antigens; RotaTeq; Rotarix; VP8*