title: Evaluation of Antimicrobial Photodynamic Therapy against Streptococcus mutans Biofilm in situ.
abstract: OBJECTIVE
  This study investigated the effect of antimicrobial photo-dynamic therapy (aPDT) over Streptococcus mutans biofilm.
  
  
  METHODS
  Eighteen (n = 18) patients were selected and one palatine device with dental blocks was used. The biofilm was treated by curcumin and Photogem® with a LED and the effect was analyzed by CFU/ml.
  
  
  RESULTS
  Although, statistical analysis showed significant reductions for aPDT mainly with Photogem® (p = 0.02), these were low.
  
  
  CONCLUSIONS
  The results suggest a low antimicrobial effect of aPDT over S. mutans biofilm. Some parameters used need to be improved.
  
  
  CONCLUSIONS
  This technique can be a promising in Dentistry.
authors.affiliation: University of São Paulo-USP, Physics Institute of São Carlos-IFSC, Optical Group, Biophotonics Laboratory; Federal University of São Carlos-UFSCar, Biotechnology Postgraduate Program, Sao Carlos, São Paulo, Brazil.
authors.forename: V H
authors.initials: VH
authors.lastname: Panhóca
authors.affiliation: University of São Paulo-USP, Physics Institute of São Carlos-IFSC, Optical Group, Biophotonics Laboratory, São Carlos Department of Restorative Dentistry, Univ Estadual Paulista-UNESP, Araraquara School of Dentistry, Araraquara São Paulo Brazil.
authors.forename: Fle
authors.initials: F
authors.lastname: Florez
authors.affiliation: Department of Restorative Dentistry, Univ Estadual Paulista-UNESP, Araraquara School of Dentistry, Araraquara, São Paulo Brazil.
authors.forename: N
authors.initials: N
authors.lastname: Batista de Faria
authors.affiliation: Professor, University of São Paulo-USP, Physics Institute of São Carlos-IFSC, Optical Group, Biophotonics Laboratory, São Carlos Department of Restorative Dentistry, Univ Estadual Paulista-UNESP, Araraquara School of Dentistry, Humaitá St 1680, Araraquara, São Paulo, Brazil ZipCode: 14.801-903 Phone: +55 (016) 3301-6393, e-mail address: alrastelli@foar.unesp.br.
authors.forename: Alessandra Nara de Souza
authors.initials: AN
authors.lastname: Rastelli
authors.affiliation: Department of Restorative Dentistry, Univ Estadual Paulista-UNESP, Araraquara School of Dentistry, Araraquara, São Paulo Brazil.
authors.forename: Jmg
authors.initials: J
authors.lastname: Tanomaru
authors.affiliation: University of São Paulo-USP, Physics Institute of São Carlos-IFSC, Optical Group, Biophotonics Laboratory, São Carlos, São Paulo, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Kurachi
authors.affiliation: University of São Paulo-USP, Physics Institute of São Carlos-IFSC, Optical Group, Biophotonics Laboratory, São Carlos, São Paulo, Brazil.
authors.forename: V S
authors.initials: VS
authors.lastname: Bagnato
mesh_terms: D000293:Adolescent; D018441:Biofilms / Q000187:drug effects* / Q000528:radiation effects*; D003474:Curcumin / Q000627:therapeutic use; D005260:Female; D006415:Hematoporphyrins / Q000627:therapeutic use; D006801:Humans; D008297:Male; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use; D013295:Streptococcus mutans / Q000302:isolation & purification
chemical_list: D006415:Hematoporphyrins; C000600680:Photogem; D017319:Photosensitizing Agents; D003474:Curcumin
keywords: Biofilm; Curcumin; Dental caries; Photodynamic therapy; Randomized clinical trial; Streptococcus mutans.