title: Short- and Long-term Evaluation of Dentin-Resin Interfaces Formed by Etch-and-Rinse Adhesives on Plasma-treated Dentin.
abstract: OBJECTIVE
  To investigate the influence of atmospheric pressure plasma (APP) treatment on the microtensile dentin bond strength of two etch-and-rinse adhesive systems, after one week and one year of water storage, and additionally to observe the micromorphology of resin/dentin interfaces under scanning electronic microscopy (SEM).
  
  
  METHODS
  The occlusal enamel was removed from third human molars to expose a flat dentin surface. The teeth were then randomly divided into six groups (n = 7), according to two adhesives (Optibond FL and XP-Bond) and three APP treatments (untreated dentin [control], APP application before or after acid etching). After performing the composite resin buildup on bonded dentin, the teeth were sectioned perpendicularly to the bonded interface to obtain beam-shaped specimens (cross-sectional area of ~0.9 mm2). The specimens were tested in tension until failure after one week and one year of water storage (1.0 mm/min rate). Bond strength data were analyzed by three-way ANOVA and Tukey's post-hoc test (α = 0.05%). Bonded beam specimens from each tooth were also prepared for interfacial SEM investigation.
  
  
  RESULTS
  At one week, APP treatment applied after acid etching increased the dentin bond strength for XP Bond, while no effect was observed for Optibond FL. After one year, the bond strength of XP Bond decreased in groups where APP was applied after etching. The evaluation time did not influence the bond strength for Optibond FL.
  
  
  CONCLUSIONS
  One-year evaluation did not show any sign of degradation of interfacial structures in any group. Application of APP to etched dentin combined with a two-step etch-and-rinse adhesive significantly increased bond strength at one week, but the effect was not stable after one year and was adhesive dependent.
authors.forename: Ronaldo
authors.initials: R
authors.lastname: Hirata
authors.forename: Camila
authors.initials: C
authors.lastname: Sampaio
authors.forename: Lucas S
authors.initials: LS
authors.lastname: Machado
authors.forename: Paulo G
authors.initials: PG
authors.lastname: Coelho
authors.forename: Van P
authors.initials: VP
authors.lastname: Thompson
authors.forename: Simone
authors.initials: S
authors.lastname: Duarte
authors.forename: Ana Paula Almeida
authors.initials: AP
authors.lastname: Ayres
authors.forename: Marcelo
authors.initials: M
authors.lastname: Giannini
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D001128:Argon / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003764:Dental Materials / Q000737:chemistry; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D058626:Plasma Gases / Q000737:chemistry*; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C469607:OptiBond FL; D058626:Plasma Gases; D019279:Resin Cements; C532387:XP-Bond; D014867:Water; D001128:Argon