title: Glycosyltransferase Gene Expression Profiles Classify Cancer Types and Propose Prognostic Subtypes.
abstract: Aberrant glycosylation in tumours stem from altered glycosyltransferase (GT) gene expression but can the expression profiles of these signature genes be used to classify cancer types and lead to cancer subtype discovery? The differential structural changes to cellular glycan structures are predominantly regulated by the expression patterns of GT genes and are a hallmark of neoplastic cell metamorphoses. We found that the expression of 210 GT genes taken from 1893 cancer patient samples in The Cancer Genome Atlas (TCGA) microarray data are able to classify six cancers; breast, ovarian, glioblastoma, kidney, colon and lung. The GT gene expression profiles are used to develop cancer classifiers and propose subtypes. The subclassification of breast cancer solid tumour samples illustrates the discovery of subgroups from GT genes that match well against basal-like and HER2-enriched subtypes and correlates to clinical, mutation and survival data. This cancer type glycosyltransferase gene signature finding provides foundational evidence for the centrality of glycosylation in cancer.
authors.affiliation: Scientific Computing Research Unit, Faculty of Science, University of Cape Town, Rondebosch, 7701, South Africa.|Department of Chemistry, Faculty of Science, University of Cape Town, Rondebosch, 7701, South Africa.
authors.forename: Jahanshah
authors.initials: J
authors.lastname: Ashkani
authors.affiliation: Scientific Computing Research Unit, Faculty of Science, University of Cape Town, Rondebosch, 7701, South Africa.|Department of Chemistry, Faculty of Science, University of Cape Town, Rondebosch, 7701, South Africa.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Naidoo
mesh_terms: D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D016695:Glycosyltransferases / Q000235:genetics*; D006801:Humans; D008297:Male; D009154:Mutation; D009369:Neoplasms / Q000145:classification* / Q000175:diagnosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D025341:Principal Component Analysis; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D016695:Glycosyltransferases