title: Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories.
abstract: Animal morphogenesis requires folds or clefts to separate populations of cells which are often associated with different cell affinities. In the Drosophila wing imaginal disc, the regional expression of the Iroquois complex (Iro-C) in the notum leads to the formation of the hinge/notum (H/N) fold that separates the wing hinge and notum territories. Although Decapentaplegic (Dpp) signaling has been revealed as essential for the hinge/notum subdivision through the restriction of Iro-C toward the notum region, the mechanism by which the H/N border develops into a fold is unknown. Here, we report that a Dpp target gene, optomotor-blind (omb), mediates the role of Dpp signaling in Iro-C inhibition. omb is complementarily expressed on the dorsal hinge side, abutting the Iro-C domain along the H/N border. Ectopic omb expression inhibits Iro-C in the notum territory, independent of known Iro-C regulators Msh and Stat92E. Uniform manipulation of either omb or Iro-C genes spanning the presumptive H/N border significantly suppresses H/N fold formation via inhibition of the apical microtubule enrichment. Ectopically sharp border or discontinuity in level of Iro-C or Omb is enough to generate ectopic fold formation. These results reveal that omb and Iro-C not only are complementarily expressed but also cooperate to promote H/N fold formation. Our data help to understand how Dpp signaling is interpreted region-specifically during tissue subdivision.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Suning
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China. Electronic address: shenjie@cau.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D004330:Drosophila; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D060227:Imaginal Discs / Q000196:embryology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D020825:T-Box Domain Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014921:Wings, Animal / Q000033:anatomy & histology / Q000196:embryology*
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D020825:T-Box Domain Proteins; C099258:caup protein, Drosophila; C064196:dpp protein, Drosophila; C072962:bi protein, Drosophila
keywords: Drosophila; Hinge notum fold; Iroquois complex; Wing disc; optomotor‐blind