title: Cell-Imprinted Substrates Modulate Differentiation, Redifferentiation, and Transdifferentiation.
abstract: Differentiation of stem cells into mature cells through the use of physical approaches is of great interest. Here, we prepared smart nanoenvironments by cell-imprinted substrates based on chondrocytes, tenocytes, and semifibroblasts as templates and demonstrated their potential for differentiation, redifferentiation, and transdifferentiation. Analysis of shape and upregulation/downregulation of specific genes of stem cells, which were seeded on these cell-imprinted substrates, confirmed that imprinted substrates have the capability to induce specific shapes and molecular characteristics of the cell types that were used as templates for cell-imprinting. Interestingly, immunofluorescent staining of a specific protein in chondrocytes (i.e., collagen type II) confirmed that adipose-derived stem cells, semifibroblasts, and tenocytes can acquire the chondrocyte phenotype after a 14 day culture on chondrocyte-imprinted substrates. In summary, we propose that common polystyrene tissue culture plates can be replaced by this imprinting technique as an effective and promising way to regulate any cell phenotype in vitro with significant potential applications in regenerative medicine and cell-based therapies.
authors.affiliation: National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran.
authors.forename: Shahin
authors.initials: S
authors.lastname: Bonakdar
authors.affiliation: Department of Nanotechnology & Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences , P.O. Box 14155-6451, Tehran, Iran.|Department of Anesthesiology, Brigham and Women's Hospital, Harvard Medical School , Boston, Massachusetts 02115, United States.
authors.forename: Morteza
authors.initials: M
authors.lastname: Mahmoudi
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR , Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Montazeri
authors.affiliation: Laboratory of Microsystems (LMIS4), École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Taghipoor
authors.affiliation: Laboratory of Microsystems (LMIS4), École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bertsch
authors.affiliation: National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, Department of Biomaterials Science and Technology, University of Twente , P.O. Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Sharifi
authors.affiliation: National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Majidi
authors.affiliation: Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Omid
authors.initials: O
authors.lastname: Mashinchian
authors.affiliation: National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hamrang Sekachaei
authors.affiliation: National Cell Bank, Pasteur Institute of Iran , P.O. Box 1316943551, Tehran, Iran.
authors.forename: Pegah
authors.initials: P
authors.lastname: Zolfaghari
authors.affiliation: Laboratory of Microsystems (LMIS4), École Polytechnique Fédérale de Lausanne , Station 17, CH-1015 Lausanne, Switzerland.
authors.forename: Philippe
authors.initials: P
authors.lastname: Renaud
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D054338:Cell Transdifferentiation / Q000235:genetics; D002478:Cells, Cultured; D024043:Collagen Type II; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*
chemical_list: D024043:Collagen Type II
keywords: ADSCs; cell imprinting; chondrogenic differentiation; redifferentiation; tenogenic differentiation; transdifferentiation