title: A novel glutathione-S transferase immunosensor based on horseradish peroxidase and double-layer gold nanoparticles.
abstract: GSTs, a biotransformation enzyme group, can perform metabolism, drug transfer and detoxification functions. Rapid detection of the GSTs with more sensitive approaches is of great importance. In the current study, a novel double-layer gold nanoparticles-electrochemical immunosensor electrode (DGN-EIE) immobilized with Glutathione S-Transferase (GST) antibody derived from Balb/c mice was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, transmission electron microscope (TEM) was used to characterize the nanogold solution. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine the GST in PBS. The results showed that the response current had a good linear correlation with the GST concentration ranged from 0.1-10(4) pg/mL. The lowest detection limit was found at 0.03 pg/mL(S/N = 3). The linear equation was deduced as △I/% = 7.386lgC + 22.36 (R(2) = 0.998). Moreover, it was validated with high sensitivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the GST.
authors.affiliation: Key Laboratory of Industrial Fermention Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.|Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300314, China.
authors.forename: Dingqiang
authors.initials: D
authors.lastname: Lu
authors.affiliation: Key Laboratory of Industrial Fermention Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
authors.forename: Fuping
authors.initials: F
authors.lastname: Lu
authors.affiliation: Key Laboratory of Industrial Fermention Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. pgc@tjcu.edu.cn.|Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300314, China. pgc@tjcu.edu.cn.
authors.forename: Guangchang
authors.initials: G
authors.lastname: Pang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D005982:Glutathione Transferase / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010640:Phenothiazines / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D010640:Phenothiazines; D006046:Gold; D048271:Chitosan; D006735:Horseradish Peroxidase; D005982:Glutathione Transferase; C009469:thionine
keywords: Electrochemical immunosensor; GNPs; GST; The amperometric I-t curve method