title: Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
abstract: The development of the patch clamp technique has enabled investigators to directly measure gap junction conductance between isolated pairs of small cells with resolution to the single channel level. The dual patch clamp recording technique requires specialized equipment and the acquired skill to reliably establish gigaohm seals and the whole cell recording configuration with high efficiency. This chapter describes the equipment needed and methods required to achieve accurate measurement of macroscopic and single gap junction channel conductances. Inherent limitations with the dual whole cell recording technique and methods to correct for series access resistance errors are defined as well as basic procedures to determine the essential electrical parameters necessary to evaluate the accuracy of gap junction conductance measurements using this approach.
authors.affiliation: Department of Pharmacology, SUNY Upstate Medical University, 3162 Weiskotten Hall, 766 Irving Ave., Syracuse, NY, 13210, USA. veenstrr@upstate.edu.|Department Cell and Developmental Biology, SUNY Upstate Medical University, 3162 Weiskotten Hall, 766 Irving Ave., Syracuse, NY, 13210, USA. veenstrr@upstate.edu.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Veenstra
mesh_terms: D000818:Animals; D017630:Connexins / Q000502:physiology*; D004553:Electric Conductivity; D017629:Gap Junctions / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods; D061251:Primary Cell Culture
chemical_list: D017630:Connexins
keywords: Channel conductance; Dual whole cell configuration; Gap junction conductance; Membrane potential; Patch clamp; Perforated patch; Series resistance; Transjunctional voltage