title: Suppression of charcoal rot in soybean by moderately halotolerant Pseudomonas aeruginosa GS-33 under saline conditions.
abstract: Charcoal rot severely limits the soybean crop yield under saline conditions. The present studies focus on biocontrol and plant growth promoting potential of phenazine producing moderately halotolerant Pseudomonas aeruginosa (GS-33) in soybean under saline soil conditions. A marine isolate; GS-33 was identified as P. aeruginosa based on polyphasic characterization. This strain showed potent in vitro biocontrol activity against charcoal rot causing fungus Macrophomina phaseolina. It was capable of producing phenazine-1-carboxylic acid even at elevated salt concentrations. Moreover, GS-33 possessed other biocontrol traits like production of siderophores, HCN and protease under saline conditions. Multiple traits for plant growth promotion such as synthesis of IAA, NH3 , and solubilization of phosphate were also exhibited by GS-33. Plant growth promoting and biocontrol control potentials of GS-33 were evaluated by pot assay under saline soil conditions. Higher biomass and chlorophyll content were observed in GS-33 treated seedlings. A greater reduction in charcoal rot caused by fungal pathogens under both normal and saline soil conditions in GS-33 treated seedlings was observed. In a nut shell, phenazine producing halotolerant strain GS-33 could mitigate saline soil conditions (abiotic stress) and infestation of M. phaseolina (biotic stress) in soybean.
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Patil
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Jayasinh
authors.initials: J
authors.lastname: Paradeshi
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Bhushan
authors.initials: B
authors.lastname: Chaudhari
mesh_terms: D001203:Ascomycota / Q000254:growth & development* / Q000472:pathogenicity; D061046:Biological Control Agents; D018533:Biomass; D019807:Iodoacetic Acid / Q000378:metabolism; D010447:Peptide Hydrolases / Q000096:biosynthesis; D010619:Phenazines / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D055049:Salt Tolerance; D017262:Siderophores / Q000378:metabolism*; D013025:Glycine max / Q000382:microbiology*
chemical_list: D061046:Biological Control Agents; D010619:Phenazines; D017262:Siderophores; C037165:1-phenazinecarboxylic acid; D010447:Peptide Hydrolases; D019807:Iodoacetic Acid
keywords: Charcoal rot suppression; Macrophomina phaseolina; Phenazine; Pseudomonas aeruginosa; Saline soil