title: TBBPA chronic exposure produces sex-specific neurobehavioral and social interaction changes in adult zebrafish.
abstract: The toxicity of tetrabromobisphenol A (TBBPA) has been extensively studied because of its high production volume. TBBPA is toxic to aquatic fish based on acute high concentration exposure tests, and few studies have assessed the behavioral effects of low concentration chronic TBBPA exposures in aquatic organisms. The present study defined the developmental and neurobehavioral effects associated with exposure of zebrafish to 0, 5 and 50nM TBBPA during 1-120days post-fertilization (dpf) following by detoxification for four months before the behaviors assessment. These low concentration TBBPA exposures were not associated with malformations and did not alter sex ratio, but resulted in reduced zebrafish body weight and length. Adult behavioral assays indicated that TBBPA exposed males had significantly higher average swim speeds and spent significantly more time in high speed darting mode and less time in medium cruising mode compared to control males. In an adult photomotor response assay, TBBPA exposure was associated with hyperactivity in male fish. Female zebrafish responses in these assays followed a similar trend, but the magnitude of TBBPA effects was generally smaller than in males. Social interaction evaluated using a mirror attack test showed that 50nM TBBPA exposed males had heightened aggression. Females exposed to 50nM TBBPA spent more time in the vicinity of the mirror, but did not show increased aggression toward the mirror compared to unexposed control fish. Overall, the hyperactivity and social behavior deficits ascribed here to chronic TBBPA exposure was most profound in males. Our findings indicate that TBBPA can cause developmental and neurobehavioral deficits, and may pose significant health risk to humans.
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, No. 130, Mei Long Road, Shanghai 200237, China; Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jiangfei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, The Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97333, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
authors.affiliation: Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory, The Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97333, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Simonich
authors.affiliation: Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Shangfei
authors.initials: S
authors.lastname: Nie
authors.affiliation: Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Lelin
authors.initials: L
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Chenglian
authors.initials: C
authors.lastname: Bai
authors.affiliation: Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Qiaoxiang
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: cjhuang5711@163.com.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Huang
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, No. 130, Mei Long Road, Shanghai 200237, China. Electronic address: kflin@ecust.edu.cn.
authors.forename: Kuangfei
authors.initials: K
authors.lastname: Lin
mesh_terms: D000374:Aggression / Q000187:drug effects; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D005260:Female; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D011075:Polybrominated Biphenyls / Q000633:toxicity*; D012919:Social Behavior; D015027:Zebrafish
chemical_list: D011075:Polybrominated Biphenyls; C020806:tetrabromobisphenol A
keywords: Adult behavior; Chronic exposure; Mirror attack; TBBPA; Zebrafish