title: Complete mitochondrial genome of Angiostrongylus malaysiensis lungworm and molecular phylogeny of Metastrongyloid nematodes.
abstract: Angiostrongylus malaysiensis is a nematode parasite of various rat species. When first documented in Malaysia, it was referred to as A. cantonensis. Unlike A. cantonensis, the complete mitochondrial genome of A. malaysiensis has not been documented. We report here its complete mitogenome, its differentiation from A. cantonensis, and the phylogenetic relationships with its congeners and other Metastrongyloid taxa. The whole mitogenome of A. malaysiensis had a total length of 13,516bp, comprising 36 genes (12 PCGs, 2 rRNA and 22 tRNA genes) and a control region. It is longer than that of A. cantonensis (13,509bp). Its control region had a long poly T-stretch of 12bp which was not present in A. cantonensis. A. malaysiensis and A. cantonensis had identical start codon for the 12 PCGs, but four PCGs (atp6, cob, nad2, nad6) had different stop codon. The cloverleaf structure for the 22 tRNAs was similar in A. malaysiensis and A. cantonensis except the TΨC-arm was absent in trnV for A. malaysiensis but present in A. cantonensis. The Angiostrongylus genus was monophyletic, with A. malaysiensis and A. cantonensis forming a distinct lineage from that of A. costaricensis and A. vasorum. The genetic distance between A. malaysiensis and A. cantonensis was p=11.9% based on 12 PCGs, p=9.5% based on 2 rRNA genes, and p=11.6% based on 14 mt-genes. The mitogenome will prove useful for studies on phylogenetics and systematics of Angiostrongylus lungworms and other Metastrongyloid nematodes.
authors.affiliation: Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia; Chancellery High Impact Research, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Hoi-Sen
authors.initials: HS
authors.lastname: Yong
authors.affiliation: Chancellery High Impact Research, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sze-Looi
authors.initials: SL
authors.lastname: Song
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Electronic address: praphathip.eam@mahidol.ac.th.
authors.forename: Praphathip
authors.initials: P
authors.lastname: Eamsobhana
authors.affiliation: Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Phaik-Eem
authors.initials: PE
authors.lastname: Lim
mesh_terms: D000801:Angiostrongylus / Q000145:classification* / Q000235:genetics*; D000818:Animals; D004285:Dogs; D054629:Genome, Mitochondrial; D008296:Malaysia; D008681:Metastrongyloidea / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*; D051381:Rats
chemical_list: D012335:RNA, Ribosomal
keywords: Angiostrongylus malaysiensis; Metastrongyloidea; Mitogenome phylogeny; Next generation sequencing