title: Localization and interactions between Arabidopsis auxin biosynthetic enzymes in the TAA/YUC-dependent pathway.
abstract: The growth regulator auxin is involved in all key developmental processes in plants. A complex network of a multiplicity of potential biosynthetic pathways as well as transport, signalling plus conjugation and deconjugation lead to a complex and multifaceted system system for auxin function. This raises the question how such a system can be effectively organized and controlled. Here we report that a subset of auxin biosynthetic enzymes in the TAA/YUC route of auxin biosynthesis is localized to the endoplasmic reticulum (ER). ER microsomal fractions also contain a significant percentage of auxin biosynthetic activity. This could point toward a model of auxin function using ER membrane location and subcellular compartmentation for supplementary layers of regulation. Additionally we show specific protein-protein interactions between some of the enzymes in the TAA/YUC route of auxin biosynthesis.
authors.affiliation: Plant Cell Biology, Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK vkriechbaumer@brookes.ac.uk.
authors.forename: Verena
authors.initials: V
authors.lastname: Kriechbaumer
authors.affiliation: Central Laser Facility, Science and Technology Facilities Council (STFC) Rutherford Appleton Laboratory, Research Complex at Harwell, Didcot OX11 0QX, UK.
authors.forename: Stanley W
authors.initials: SW
authors.lastname: Botchway
authors.affiliation: Plant Cell Biology, Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Hawes
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D010105:Oxygenases / Q000502:physiology*; D010937:Plant Growth Regulators / Q000096:biosynthesis* / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010105:Oxygenases; C422746:YUC protein, Arabidopsis
keywords: Arabidopsis; YUCCA.; auxin biosynthesis; compartmentation; endoplasmic reticulum; localization; metabolon; protein–protein interaction; thaliana