title: Isolation, identification, and characterization of novel nanovesicles.
abstract: Extracellular microvesicles (EVs) have been recognized for many potential clinical applications including biomarkers for disease diagnosis. In this study, we identified a major population of EVs by simply screening fluid samples with a nanosizer. Unlike other EVs, this extracellular nanovesicle (named HG-NV, HG-NV stands for HomoGenous nanovesicle as well as for Huang-Ge- nanovesicle) can be detected with a nanosizer with minimal in vitro manipulation and are much more homogenous in size (8-12 nm) than other EVs. A simple filtration platform is capable of separating HG-NVs from peripheral blood or cell culture supernatants. In comparison with corresponding exosome profiles, HG-NVs released from both mouse and human breast tumor cells are enriched with RNAs. Tumor derived HG-NVs are more potent in promoting tumor progression than exosomes. In summary, we identified a major subset of EVs as a previously unrecognized nanovesicle. Tumor cell derived HG-NVs promote tumor progression. Molecules predominantly present in breast tumor HG-NVs have been identified and characterized. This discovery may have implications in advancing both microvesicle biology research and clinical management including potential used as a biomarker.
authors.affiliation: Louisville Veterans Administration Medical Center, Louisville, KY 40206, USA.|James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.
authors.forename: Huang-Ge
authors.initials: HG
authors.lastname: Zhang
authors.affiliation: James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.
authors.forename: Pengxiao
authors.initials: P
authors.lastname: Cao
authors.affiliation: James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Teng
authors.affiliation: Program in Biostatistics, Bioinformatics and Systems Biology, The University of Texas Graduate School of Biomedical Sciences at Houston, TX 77030, USA.|Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.affiliation: James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.|Department of Clinical Oncology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, 223300, China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Translational Genomics Research Institute, Phoenix, AZ 85004, USA.
authors.forename: Ashish S
authors.initials: AS
authors.lastname: Yeri
authors.affiliation: James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.
authors.forename: Abhilash
authors.initials: A
authors.lastname: Samykutty
authors.affiliation: James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.
authors.forename: Jingyao
authors.initials: J
authors.lastname: Mu
authors.affiliation: Department of Medicine, University of Louisville, KY 40202, USA.
authors.forename: Zhong-Bin
authors.initials: ZB
authors.lastname: Deng
authors.affiliation: James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Mass Spectrometry/Proteomics Shared Facility, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Mobley
authors.affiliation: Department of Medicine, University of Louisville, KY 40202, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: Translational Genomics Research Institute, Phoenix, AZ 85004, USA.
authors.forename: Kendall
authors.initials: K
authors.lastname: Van Keuren-Jensen
authors.affiliation: Department of Medicine, University of Louisville, KY 40202, USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Miller
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D001774:Blood Chemical Analysis / Q000379:methods; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002458:Cell Fractionation / Q000379:methods; D045744:Cell Line, Tumor; D055252:Cell-Derived Microparticles / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D055354:Exosomes / Q000235:genetics / Q000473:pathology; D000067128:Extracellular Vesicles / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000032:analysis; D009367:Neoplasm Staging / Q000379:methods; D011237:Predictive Value of Tests; D020543:Proteome / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome
keywords: HG-NV; exosomes; in vivo predominately population; isolation and identification extracellular microvesicles