title: New monoclonal antibodies to the Ebola virus glycoprotein: Identification and analysis of the amino acid sequence of the variable domains.
abstract: We determined the nucleotide and amino acid sequences of variable domains of three new monoclonal antibodies to the glycoprotein of Ebola virus capsid. The framework and hypervariable regions of immunoglobulin heavy and light chains were identified. The primary structures were confirmed using massspectrometry analysis. Immunoglobulin database search showed the uniqueness of the sequences obtained.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia. panina@mx.ibch.ru.
authors.forename: A A
authors.initials: AA
authors.lastname: Panina
authors.affiliation: Faculty of Chemistry, Moscow State University, Moscow, 119991, Russia.
authors.forename: T K
authors.initials: TK
authors.lastname: Aliev
authors.affiliation: All-Russia Research Center of Molecular Diagnostics and Therapy, Simferopol'skii bul'var 8, Moscow, 117149, Russia.
authors.forename: O B
authors.initials: OB
authors.lastname: Shemchukova
authors.affiliation: All-Russia Research Center of Molecular Diagnostics and Therapy, Simferopol'skii bul'var 8, Moscow, 117149, Russia.
authors.forename: I G
authors.initials: IG
authors.lastname: Dement'yeva
authors.affiliation: All-Russia Research Center of Molecular Diagnostics and Therapy, Simferopol'skii bul'var 8, Moscow, 117149, Russia.
authors.forename: N E
authors.initials: NE
authors.lastname: Varlamov
authors.affiliation: All-Russia Research Center of Molecular Diagnostics and Therapy, Simferopol'skii bul'var 8, Moscow, 117149, Russia.
authors.forename: L P
authors.initials: LP
authors.lastname: Pozdnyakova
authors.affiliation: All-Russia Research Center of Molecular Diagnostics and Therapy, Simferopol'skii bul'var 8, Moscow, 117149, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Bokov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.|Faculty of Biology, Moscow State University, Moscow, 119991, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Dolgikh
authors.affiliation: All-Russia Research Center of Molecular Diagnostics and Therapy, Simferopol'skii bul'var 8, Moscow, 117149, Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Sveshnikov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.|Faculty of Biology, Moscow State University, Moscow, 119991, Russia.
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000235:genetics; D000914:Antibodies, Viral / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D029043:Ebolavirus / Q000276:immunology*; D004586:Electrophoresis; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D017931:DNA Primers; D007135:Immunoglobulin Variable Region; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus