title: SPR imaging biosensor for determination of laminin-5 as a potential cancer marker in biological material.
abstract: A new method for the selective determination of laminin-5 concentration using a biosensor and surface plasmon resonance imaging (SPRI) technique is presented. A biosensor based on the specific interaction of laminin-5 with rabbit polyclonal antibody was constructed. The analytically useful dynamic response range of the biosensor is between 0.014 and 0.1 ng mL(-1). The detection limit is 4 pg mL(-1). The potential influence of interferences on the SPRI signal was investigated, and the high selectivity of the biosensor was confirmed. In order to demonstrate the potential application of the biosensor, laminin-5 concentration in blood plasma was determined. The results were compared with the laminin-5 concentration obtained by the commercial enzyme-linked immunosorbent assay (ELISA) kit. A comparison of results from healthy donors obtained by SPRI measurement and ELISA indicates that they are close and shows good agreement with the data reported in the literature. The plasma samples of bladder cancer patients gave higher concentration measured with specific biosensor than by ELISA assay. The study shows the clear difference in concentration of laminin-5 in healthy humans and patients with bladder cancer. Extensive clinical studies using the newly developed method can result in an increase in the use of laminin-5 as a potential cancer marker.
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sankiewicz
authors.affiliation: Department of Medical Biochemistry, Medical University of Bialystok, A. Mickiewicza 2C, 15-089, Bialystok, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Romanowicz
authors.affiliation: Department of Perinatology, Medical University of Bialystok, M. Sklodowskiej-Curie 24A, 15-276, Bialystok, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Laudanski
authors.affiliation: Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Waszyngtona 17, 15-269, Bialystok, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Zelazowska-Rutkowska
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Puzan
authors.affiliation: Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Waszyngtona 17, 15-269, Bialystok, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Cylwik
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland. ewka@uwb.edu.pl.
authors.forename: E
authors.initials: E
authors.lastname: Gorodkiewicz
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation*; D015815:Cell Adhesion Molecules / Q000097:blood*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D001749:Urinary Bladder Neoplasms / Q000097:blood*; D000097794:Kalinin
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000097794:Kalinin
keywords: Biosensors; Cancer marker; Laminin-5; Surface plasmon resonance imaging