title: Reprint of: Effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte Fusarium solani.
abstract: Volumetric productivity of camptothecin from the suspension culture of the endophyte Fusarium solani was enhanced up to ∼152 fold (from 0.19μgl(-1)d(-1) to 28.9μgl(-1)d(-1)) under optimized fermentation conditions including initial pH (6.0), temperature (32°C) and agitation speed (80rpm) with (5% (v/v)) ethanol as medium component. Among various elicitors and precursors studied, tryptamine (0.5mM) as precursor and bovine serum albumin (BSA) (0.075mM) as an elicitor added on day 6 of the cultivation period resulted in maximum enhancement of camptothecin concentration (up to 4.5 and 3.4-fold, respectively). These leads provide immense scope for further enhancement in camptothecin productivity at bioreactor level. The cytotoxicity analysis of the crude camptothecin extract from the fungal biomass revealed its high effectiveness against colon and mammary gland cancer cell lines.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Aarthi
authors.initials: A
authors.lastname: Venugopalan
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Uma Rani
authors.initials: UR
authors.lastname: Potunuru
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Madhulika
authors.initials: M
authors.lastname: Dixit
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India. Electronic address: smita@iitm.ac.in.
authors.forename: Smita
authors.initials: S
authors.lastname: Srivastava
keywords: Camptothecin; Elicitors; Fermentation parameters; Fusarium solani; Precursors