title: Enhancing enzymolysis and fermentation efficiency of sugarcane bagasse by synergistic pretreatment of Fenton reaction and sodium hydroxide extraction.
abstract: A study on the synergistic pretreatment of sugarcane bagasse (SCB) using Fenton reaction and NaOH extraction was conducted. The optimized process conditions for Fenton pretreatment were 10% (w/w) of H2O2, 20mM of Fe(2+), pH 2.5, pretreatment time 6h, and pretreatment temperature 55°C. Sequential pretreatments were performed in combination with NaOH extraction (NaOH 1% (w/w), 80°C, 5% of solid loading, 1h). Among all the pretreatments, Fenton pretreatment followed by NaOH extraction had the highest efficiency of 64.7% and 108.3% for enzymolysis and simultaneous saccharification fermentation (SSF) with an ethanol concentration of 17.44g/L. The analyses by the scanning electron microscopy, X-ray diffraction and confocal laser scanning microscopy revealed that Fenton pretreatment disrupts the structure of SCB to facilitate the degradation of lignin by NaOH. The overall data suggest that this combinatorial strategy is a promising process for SCB pretreatment.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China; Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou 510006, People's Republic of China. Electronic address: mjzhu@scut.edu.cn.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Zhu
mesh_terms: D001709:Biotechnology; D002482:Cellulose / Q000737:chemistry*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007501:Iron / Q000737:chemistry; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D031786:Saccharum / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry*; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: C045076:Fenton's reagent; D000431:Ethanol; D012972:Sodium Hydroxide; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Enzymolysis; Fenton reaction; NaOH extraction; Simultaneous saccharification fermentation (SSF); Sugarcane bagasse (SCB); Synergistic pretreatment