title: Nasal Tumorigenesis in B6C3F1 Mice Following Intraperitoneal Diethylnitrosamine.
abstract: Diethylnitrosamine (DEN) is a chemical broadly used in animal models as a hepatocarcinogen, reported to also cause pulmonary neoplasms in mice. The original objective was to evaluate the impact of a Western diet with or without 10% broccoli on DEN-induced on liver cancer. We administered DEN (45 mg/kg) intraperitoneally to young adult male B6C3F1 mice by 6 weekly injections and evaluated liver cancer 6 months after the DEN treatments. Here, we report unexpected primary tumorigenesis in nasal epithelium, independent of dietary treatment. More than 50% of DEN-treated B6C3F1 mice developed nasal neoplasm-related lesions, not reported previously in the literature. Only one of these neoplasms was visible externally prior to postmortem examination. Intraperitoneal DEN treatment used as a model for liver cancer can have a carcinogenic effect on the nasal epithelium in B6C3F1 mice, which should be carefully monitored in future liver cancer studies.
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois, Urbana, Illinois, USA.
authors.forename: Yung-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of Pathobiology, University of Illinois, Urbana, Illinois, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Wallig
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois, Urbana, Illinois, USA ejeffery@illinois.edu.
authors.forename: Elizabeth H
authors.initials: EH
authors.lastname: Jeffery
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000139:chemically induced*; D002273:Carcinogens / Q000633:toxicity*; D004048:Diethylamines / Q000633:toxicity*; D008113:Liver Neoplasms / Q000139:chemically induced; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009669:Nose Neoplasms / Q000139:chemically induced*
chemical_list: D002273:Carcinogens; D004048:Diethylamines; C031406:N-nitrodiethylamine
keywords: B6C3F1; diethylnitrosamine; nasal neoplasm