title: Molecular Architecture of  Yeast Chromatin Assembly Factor 1.
abstract: Chromatin Assembly Complex 1 (CAF-1) is a major histone chaperone involved in deposition of histone H3 and H4 into nucleosome. CAF-1 is composed of three subunits; p150, p60 and p48 for human and Cac1, Cac2 and Cac3 for yeast. Despite of its central role in chromatin formation, structural features of the full CAF-1 in complex with histones and other chaperones have not been well characterized. Here, we dissect molecular architecture of yeast CAF-1 (yCAF-1) by cross-linking mass spectrometry (XL-MS) and negative stain single-particle electron microscopy (EM). Our work revealed that Cac1, the largest subunit of yCAF-1, might serve as a major histone binding platform linking Cac2 and Cac3. In addition, EM analysis showed that yCAF-1 adopts a bilobal shape and Cac1 connecting Cac2 and Cac3 to generate a platform for binding histones. This study provides the first structural glimpse of the full CAF-1 complex and a structural framework to understand histone chaperoning processes.
authors.affiliation: Department of Biological Sciences, KAIST Institute for the BioCentury, Cancer Metastasis Control Center, KAIST, 291 Daehakro Yuseong Daejeon, 34141, Korea.
authors.forename: Daegeun
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
authors.forename: Dheva
authors.initials: D
authors.lastname: Setiaputra
authors.affiliation: Department of Biological Sciences, KAIST Institute for the BioCentury, Cancer Metastasis Control Center, KAIST, 291 Daehakro Yuseong Daejeon, 34141, Korea.|Department of Biosciences and Nutrition, Karolinska Institute, and School of Technology and Health, KTH Royal Institute of Technology, Novum, SE-141 57, Sweden.
authors.forename: Taeyang
authors.initials: T
authors.lastname: Jung
authors.affiliation: Department of Biological Sciences, KAIST Institute for the BioCentury, Cancer Metastasis Control Center, KAIST, 291 Daehakro Yuseong Daejeon, 34141, Korea.
authors.forename: Jaehee
authors.initials: J
authors.lastname: Chung
authors.affiliation: Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Leitner
authors.affiliation: Department of Biological Sciences, KAIST Institute for the BioCentury, Cancer Metastasis Control Center, KAIST, 291 Daehakro Yuseong Daejeon, 34141, Korea.
authors.forename: Jungmin
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland.|Faculty of Science, University of Zurich, Zurich, Switzerland.
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institute, and School of Technology and Health, KTH Royal Institute of Technology, Novum, SE-141 57, Sweden.
authors.forename: Hans
authors.initials: H
authors.lastname: Hebert
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
authors.forename: Calvin K
authors.initials: CK
authors.lastname: Yip
authors.affiliation: Department of Biological Sciences, KAIST Institute for the BioCentury, Cancer Metastasis Control Center, KAIST, 291 Daehakro Yuseong Daejeon, 34141, Korea.
authors.forename: Ji-Joon
authors.initials: JJ
authors.lastname: Song
mesh_terms: D056504:Chromatin Assembly Factor-1 / Q000737:chemistry*; D046912:Multiprotein Complexes / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D056504:Chromatin Assembly Factor-1; D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins