title: Purification and characterization of a high salt-tolerant alginate lyase from Cobetia sp. WG-007.
abstract: An alginate lyase producing bacterial strain, Cobetia sp. WG-007, was isolated and identified from rotting seaweed. The alginate lyase, Aly-W02, was purified by procedures of ultrafiltration, Q-Sepharose Fast Flow, Phenyl Sepharose 6 Fast Flow, and Superdex-G100 with specific activity of 21,285.5 U/mg. Aly-W02 had an apparent molecular mass of 35 kDa. It exhibited maximum activity at 45 °C in 50 mM sodium phosphate buffer (pH 8.5). This alginate lyase was stable in the pH range of 6.0-8.5. Among the tested metal ions, the addition of K+, Na+, and Mg2+ions can enhance the enzyme activities, while Ba2+, Ni+, Cu2+, Mn2+, Zn2+, Ag+, and ethylenediaminetetraacetic acid decreased the activities. It displayed high salt-tolerant ability; 0.8 M NaCl or 1.5 M KCl significantly enhanced the enzyme activity. Furthermore, Aly-W02 mainly released disaccharide, trisaccharide, and tetrasaccharid from alginate. It showed potential in producing low molecular weight alginate oligosaccharides.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Gong
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Xu-Mei
authors.initials: XM
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Geng
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Lu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Shi
mesh_terms: D044129:Halomonadaceae / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000494:pharmacology; D011133:Polysaccharide-Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D010710:Phosphates; D012965:Sodium Chloride; D011189:Potassium Chloride; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase; C018279:sodium phosphate
keywords: Cobetia sp; alginate lyase; characterization; purification; salt-tolerant