title: Nanopore formation process in artificial cell membrane induced by plasma-generated reactive oxygen species.
abstract: We investigated morphological change of an artificial lipid bilayer membrane induced by oxygen radicals which were generated by non-equilibrium atmospheric pressure plasma. Neutral oxygen species, O((3)Pj) and O2((1)Δg), were irradiated of a supported lipid bilayer existing under a buffer solution at various conditions of dose time and distances, at which the dose amounts of the oxygen species were calculated quantitatively. Observation using an atomic force microscope and a fluorescence microscope revealed that dose of the neutral oxygen species generated nanopores with the diameter of 10-50 nm in a phospholipid bilayer, and finally destructed the bilayer structure. We found that protrusions appeared on the lipid bilayer surface prior to the formation of nanopores, and we attributed the protrusions to the precursor of the nanopores. We propose a mechanism of the pore formation induced by lipid oxidation on the basis of previous experimental and theoretical studies.
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan; Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan. Electronic address: tero@tut.jp.
authors.forename: Ryugo
authors.initials: R
authors.lastname: Tero
authors.affiliation: Department of Electrical and Electronic Information Engineering, Toyohashi, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Ryuma
authors.initials: R
authors.lastname: Yamashita
authors.affiliation: Plasma Medical Science Global Innovation Center, Nagoya University, Nagoya, Aichi 464-8601, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashizume
authors.affiliation: Department of Electrical and Electronic Information Engineering, Toyohashi, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Suda
authors.affiliation: Department of Electrical and Electronic Information Engineering, Toyohashi, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Takikawa
authors.affiliation: Plasma Medical Science Global Innovation Center, Nagoya University, Nagoya, Aichi 464-8601, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Hori
authors.affiliation: Department of Electrical and Electronic Engineering, Faculty of Science and Technology, Meijo University, Nagoya, Aichi 468-8502, Japan. Electronic address: ito@meijo-u.ac.jp.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ito
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D002021:Buffers; D002462:Cell Membrane / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D008823:Micelles; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods; D010100:Oxygen / Q000737:chemistry; D010743:Phospholipids / Q000378:metabolism; D058626:Plasma Gases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D000447:Aldehydes; D002021:Buffers; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D008823:Micelles; D010743:Phospholipids; D058626:Plasma Gases; D017382:Reactive Oxygen Species; D010100:Oxygen
keywords: Atmospheric pressure plasma; Atomic force microscope; Lipid bilayer; Lipid oxidation; Reactive oxygen species