title: The Possible Roles of Biological Bone Constructed with Peripheral Blood Derived EPCs and BMSCs in Osteogenesis and Angiogenesis.
abstract: This study aimed to determine the possible potential of partially deproteinized biologic bone (PDPBB) seeded with bone marrow stromal cells (BMSCs) and endothelial progenitor cells (EPCs) in osteogenesis and angiogenesis. BMSCs and EPCs were isolated, identified, and cocultured in vitro, followed by seeding on the PDPBB. Expression of osteogenesis and vascularization markers was quantified by immunofluorescence (IF) staining, immunohistochemistry (IHC), and quantitive real-time polymerase chain reaction (qRT-PCR). Scanning electron microscope (SEM) was also employed to further evaluate the morphologic alterations of cocultured cells in the biologic bone. Results demonstrated that the coculture system combined with BMSCs and EPCs had significant advantages of (i) upregulating the mRNA expression of VEGF, Osteonectin, Osteopontin, and Collagen Type I and (ii) increasing ALP and OC staining compared to the BMSCs or EPCs only group. Moreover, IHC staining for CD105, CD34, and ZO-1 increased significantly in the implanted PDPBB seeded with coculture system, compared to that of BMSCs or EPCs only, respectively. Summarily, the present data provided evidence that PDPBB seeded with cocultured system possessed favorable cytocompatibility, provided suitable circumstances for different cell growth, and had the potential to provide reconstruction for cases with bone defection by promoting osteogenesis and angiogenesis.
authors.affiliation: Medical Imaging Department, The First Affiliated Hospital, Kunming Medical University, Kunming 650032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.affiliation: Plastic Surgery Department, The First Affiliated Hospital, Kunming Medical University, Kunming 650032, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Medical Imaging Department, The First Affiliated Hospital, Kunming Medical University, Kunming 650032, China.
authors.forename: Bo
authors.initials: B
authors.lastname: He
authors.affiliation: Medical Imaging Department, The First Affiliated Hospital, Kunming Medical University, Kunming 650032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Medical Imaging Department, The First Affiliated Hospital, Kunming Medical University, Kunming 650032, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Xie
authors.affiliation: Plastic Surgery Department, The First Affiliated Hospital, Kunming Medical University, Kunming 650032, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D016025:Bone Transplantation; D001842:Bone and Bones / Q000502:physiology* / Q000648:ultrastructure; D002469:Cell Separation; D048430:Cell Shape; D018920:Coculture Techniques; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D055806:Microvessels / Q000502:physiology; D018919:Neovascularization, Physiologic; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D015675:Osteocalcin; D000469:Alkaline Phosphatase