title: In Vitro Cytokine Expression and In Vivo Healing and Inflammatory Response to a Collagen-Coated Synthetic Bone Filler.
abstract: The goal of the present work was to investigate the relationship between in vivo healing and inflammatory response and in vitro cytokine expression by macrophages of a synthetic bone filler (25% hydroxylapatite-75% β-tricalcium phosphate) bearing a surface nanolayer of collagen. A clinically accepted, state-of-the-art xenograft material was used as a "negative control," that is, as a material that provides the correct clinical response for the intended use. In vitro data show that both materials exert a very low stimulation of proinflammatory cytokines by macrophages, and this was confirmed by the very mild inflammatory response detected in in vivo tests of local response in a rabbit model. Also, in vitro findings suggest a different mechanism of healing for the test and the control material, with a higher regenerative activity for the synthetic, resorbable filler, as confirmed by in vivo observation and literature reports. Thus, the simple in vitro model adopted provides a reasonable forecast of in vivo results, suggesting that new product development can be guided by in vitro tuning of cell-materials interactions.
authors.affiliation: Nobil Bio Ricerche S.r.l., Via Valcastellana 26, 14037 Portacomaro, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bollati
authors.affiliation: Nobil Bio Ricerche S.r.l., Via Valcastellana 26, 14037 Portacomaro, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Morra
authors.affiliation: Nobil Bio Ricerche S.r.l., Via Valcastellana 26, 14037 Portacomaro, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Cassinelli
authors.affiliation: Department of Clinico-Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, University of Pavia, P.le Golgi 2, 27100 Pavia, Italy.
authors.forename: Saturnino Marco
authors.initials: SM
authors.lastname: Lupi
authors.affiliation: Department of Clinico-Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, University of Pavia, P.le Golgi 2, 27100 Pavia, Italy.
authors.forename: Ruggero
authors.initials: R
authors.lastname: Rodriguez Y Baena
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism*; D017886:Durapatite / Q000737:chemistry*; D005264:Femoral Fractures / Q000628:therapy; D020869:Gene Expression Profiling; D064593:Heterografts / Q000737:chemistry; D007249:Inflammation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008422:Materials Testing; D011817:Rabbits; D013499:Surface Properties; D016896:Treatment Outcome
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D016207:Cytokines; C485817:beta-tricalcium phosphate; D003094:Collagen; D017886:Durapatite