title: Synthesis and application of thiol-functionalized magnetic nanoparticles for studying interactions of epirubicin hydrochloride with bovine serum albumin by fluorescence spectrometry.
abstract: A novel method was developed for studying the interaction between epirubicin hydrochloride (EPI) and bovine serum albumin (BSA) by fluorescence spectrometry. Fe3O4magnetic nanoparticles (MNPs) synthesized and functionalized with thiol group were employed for the immobilization and separation of target BSA in reaction solutions. The concentrations of the non-immobilized BSA and unbound EPI were obtained separately by fluorescence spectrometry. The binding constants (Ka) and number of binding sites (n) of EPI with BSA were calculated. In this study, the Kavalue was 5.05 × 105L mol-1, suggesting a strong binding of EPI to BSA, and the n value was 1.15. The effects of common metal ions on Kaof EPI with BSA were also investigated, and the results showed there was clearly bindings between the metal ions and BSA. The precise binding sites of EPI on BSA were determined as being in site I from the competitive displacement experiments. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
authors.forename: Huixia
authors.initials: H
authors.lastname: Xiong
authors.affiliation: School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
authors.forename: Quanping
authors.initials: Q
authors.lastname: Diao
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jin
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
authors.forename: Daqian
authors.initials: D
authors.lastname: Song
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000818:Animals; D002417:Cattle; D015251:Epirubicin / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D015251:Epirubicin
keywords: bovine serum albumin; epirubicin hydrochloride; fluorescence; thiol-functionalized magnetic nanoparticles