title: High-resolution characterization of a PACAP-EGFP transgenic mouse model for mapping PACAP-expressing neurons.
abstract: Pituitary adenylate cyclase-activating polypeptide (PACAP, gene name Adcyap1) regulates a wide variety of neurological and physiological functions, including metabolism and cognition, and plays roles in of multiple forms of stress. Because of its preferential expression in nerve fibers, it has often been difficult to trace and identify the endogenous sources of the peptide in specific populations of neurons. Here, we introduce a transgenic mouse line that harbors in its genome a bacterial artificial chromosome containing an enhanced green fluorescent protein (EGFP) expression cassette inserted upstream of the PACAP ATG translation initiation codon. Analysis of expression in brain sections of these mice using a GFP antibody reveals EGFP expression in distinct neuronal perikarya and dendritic arbors in several major brain regions previously reported to express PACAP from using a variety of approaches, including radioimmunoassay, in situ hybridization, and immunohistochemistry with and without colchicine. EGFP expression in neuronal perikarya was modulated in a manner similar to PACAP gene expression in motor neurons after peripheral axotomy in the ipsilateral facial motor nucleus in the brainstem, providing an example in which the transgene undergoes proper regulation in vivo. These mice and the high-resolution map obtained are expected to be useful in understanding the anatomical patterns of PACAP expression and its plasticity in the mouse. J. Comp. Neurol. 524:3827-3848, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Psychiatry, University of California, Los Angeles, USA 90095.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Condro
authors.affiliation: Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, USA 90095.|Brain Research Institute, University of California, Los Angeles, USA 90095.
authors.forename: Anna
authors.initials: A
authors.lastname: Matynia
authors.affiliation: Institute of Neuro Imaging and Informatics, Keck School of Medicine, University of Southern California, Los Angeles, CA USA 90089.
authors.forename: Nicholas N
authors.initials: NN
authors.lastname: Foster
authors.affiliation: Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan 565-0871.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ago
authors.affiliation: Department of Psychiatry, University of California, Los Angeles, USA 90095.|Department of Psychology, University of California, Los Angeles, USA 90095.
authors.forename: Abha K
authors.initials: AK
authors.lastname: Rajbhandari
authors.affiliation: Department of Psychiatry, University of California, Los Angeles, USA 90095.
authors.forename: Christina
authors.initials: C
authors.lastname: Van
authors.affiliation: Department of Psychiatry, University of California, Los Angeles, USA 90095.
authors.forename: Bhavaani
authors.initials: B
authors.lastname: Jayaram
authors.affiliation: Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, USA 90095.|Brain Research Institute, University of California, Los Angeles, USA 90095.
authors.forename: Sachin
authors.initials: S
authors.lastname: Parikh
authors.affiliation: Department of Psychiatry, University of California, Los Angeles, USA 90095.
authors.forename: Anna L
authors.initials: AL
authors.lastname: Diep
authors.affiliation: Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, USA 90095.|Brain Research Institute, University of California, Los Angeles, USA 90095.
authors.forename: Eileen
authors.initials: E
authors.lastname: Nguyen
authors.affiliation: Department of Neurological Sciences, University of Vermont, USA 05405.
authors.forename: Victor
authors.initials: V
authors.lastname: May
authors.affiliation: Institute of Neuro Imaging and Informatics, Keck School of Medicine, University of Southern California, Los Angeles, CA USA 90089.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Dong
authors.affiliation: Department of Psychiatry, University of California, Los Angeles, USA 90095.
authors.forename: James A
authors.initials: JA
authors.lastname: Waschek
mesh_terms: D000818:Animals; D019771:Axotomy; D001921:Brain / Q000166:cytology / Q000378:metabolism; D020220:Facial Nerve Injuries / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008822:Mice, Transgenic; D023421:Models, Animal; D009474:Neurons / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism
chemical_list: D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Adcyap1; RRID:AB_10049744; RRID:AB_10058149; RRID:AB_10073917; RRID:AB_10563748; RRID:AB_10680176; RRID:AB_177621; RRID:AB_2336201; RRID:IMSR_MMRRC:012011; RRID:SciRes_000137; RRID:nlx_143660; central nervous system; facial nerve axotomy; neuropeptide; pituitary adenylyl cyclase-activating polypeptide; reporter mouse