title: Influence of volatile organic compounds emitted by Pseudomonas and Serratia strains on Agrobacterium tumefaciens biofilms.
abstract: The ability to form biofilms plays an important role in bacteria-host interactions, including plant pathogenicity. In this work, we investigated the action of volatile organic compounds (VOCs) produced by rhizospheric strains of Pseudomonas chlororaphis 449, Pseudomonas fluorescens B-4117, Serratia plymuthica IC1270, as well as Serratia proteamaculans strain 94, isolated from spoiled meat, on biofilms formation by three strains of Agrobacterium tumefaciens which are causative agents of crown-gall disease in a wide range of plants. In dual culture assays, the pool of volatiles emitted by the tested Pseudomonas and Serratia strains suppressed the formation of biofilms of A. tumefaciens strains grown on polycarbonate membrane filters and killed Agrobacterium cells in mature biofilms. The individual VOCs produced by the tested Pseudomonas strains, that is, ketones (2-nonanone, 2-heptanone, 2-undecanone), and dimethyl disulfide (DMDS) produced by Serratia strains, were shown to kill A. tumefaciens cells in mature biofilms and suppress their formation. The data obtained in this study suggest an additional potential of some ketones and DMDS as protectors of plants against A. tumefaciens strains, whose virulence is associated with the formation of biofilms on the infected plants.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Plyuta
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Valentina
authors.initials: V
authors.lastname: Lipasova
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Popova
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.|M.V. Lomonosov Moscow State University, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Koksharova
authors.affiliation: D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kuznetsov
authors.affiliation: National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, Kecskemét, Hungary.
authors.forename: Erno
authors.initials: E
authors.lastname: Szegedi
authors.affiliation: Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
authors.forename: Leonid
authors.initials: L
authors.lastname: Chernin
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Inessa
authors.initials: I
authors.lastname: Khmel
mesh_terms: D016960:Agrobacterium tumefaciens / Q000187:drug effects* / Q000502:physiology*; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004220:Disulfides / Q000378:metabolism / Q000494:pharmacology; D007659:Ketones / Q000378:metabolism / Q000494:pharmacology; D008460:Meat / Q000382:microbiology; D050296:Microbial Viability / Q000187:drug effects; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D012705:Serratia / Q000302:isolation & purification / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D004220:Disulfides; D007659:Ketones; D055549:Volatile Organic Compounds; C021181:dimethyl disulfide
keywords: Agrobacterium tumefaciens; Biofilm; bacterial volatile organic compounds; dimethyl disulfide; ketones