title: Wet and Dry Forms of Bacterial Cellulose Synthetized by Different Strains of Gluconacetobacter xylinus as Carriers for Yeast Immobilization.
abstract: The present study aimed to explore and describe the properties of bacterial cellulose (BC) membranes obtained from three different strains of Gluconacetobacter xylinus for 72, 120, and 168 h, used as a carrier support for the immobilization of Saccharomyces cerevisiae. The experiments also included the analysis of glucose consumption and alcohol production during the fermentation process displayed by yeasts immobilized on the BC surface. The results of the present study demonstrate that the number of immobilized yeast cells is dependent on the type of cellulose-synthesizing strain, cellulose form, and duration of its synthesis. The BC in the form of wet membranes obtained after 3 days of synthesis displayed the most favorable properties as a carrier for yeast immobilization. The immobilization of yeast cells on BC, regardless of its form, increased the amount of the produced alcohol as compared to free cells. The yeast cells immobilized in BC were able to multiply on its surface during the fermentation process.
authors.affiliation: Department of Immunology, Microbiology and Physiological Chemistry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311, Szczecin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Żywicka
authors.affiliation: Department of Immunology, Microbiology and Physiological Chemistry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311, Szczecin, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Peitler
authors.affiliation: Institute of Chemical Engineering and Environmental Protection Processes, West Pomeranian University of Technology, Szczecin, Piastów 42, 71-065, Szczecin, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Rakoczy
authors.affiliation: Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wroclaw, Borowska 211a, 50-556, Wroclaw, Poland.
authors.forename: Adam Feliks
authors.initials: AF
authors.lastname: Junka
authors.affiliation: Department of Immunology, Microbiology and Physiological Chemistry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311, Szczecin, Poland. karol.fijalkowski@zut.edu.pl.
authors.forename: Karol
authors.initials: K
authors.lastname: Fijałkowski
mesh_terms: D002452:Cell Count; D018914:Cells, Immobilized / Q000166:cytology; D002482:Cellulose / Q000096:biosynthesis*; D003890:Desiccation; D005285:Fermentation; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008567:Membranes, Artificial; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D014867:Water / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D014867:Water; D002482:Cellulose; D005947:Glucose
keywords: Bacterial cellulose; Fermentation; Gluconacetobacter xylinus; Immobilization; Saccharomyces cerevisiae