title: The mucosal expression signatures of g-type lysozyme in turbot (Scophthalmus maximus) following bacterial challenge.
abstract: The mucosal surfaces constitute the first line of host defense against infection, and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes, while in constantly contact with a wide range of pathogens. The lysozymes are considered as key components for innate immune response to pathogen infection with their strong antibacterial activities. But their activities in mucosal immune responses were always overlooked, especially for g-type lysozymes, whose expression patterns in mucosal tissues following bacterial challenge are still limited. Towards to this end, here, we characterized the g-type lysozymes, Lyg1 and Lyg2 in turbot, and determined their expression patterns in mucosal barriers following different bacterial infection. The phylogenetic analysis revealed the turbot g-type lysozyme genes showed the closest relationship to Cynoglossus semilaevis. The two lysozyme genes showed different expression patterns following challenge. Lyg2 was significantly up-regulated in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge, while Lyg1 showed a general trend of down-regulation. The significant mucosal expression signatures of g-type lysozyme genes indicated their key roles to prevent pathogen attachment and entry in the first line of host defense system. Further functional studies should be carried out to better characterize the availability of utilization of g-type lysozyme to increase the disease resistance in the mucosal surfaces and facilitate the disease resistant breeding selection.
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Yichao
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Dong
authors.affiliation: Ministry of Agriculture Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China.
authors.forename: Baofeng
authors.initials: B
authors.lastname: Su
authors.affiliation: Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: leoochao@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005393:Fish Diseases / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018928:Immunity, Mucosal / Q000235:genetics; D009113:Muramidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment; D013290:Streptococcal Infections / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D000070058:Streptococcus iniae / Q000502:physiology; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
chemical_list: D029941:Fish Proteins; D009113:Muramidase
keywords: G-type lysozyme; Mucosal immunity; Streptococcus iniae infection; Turbot; Vibrio anguillarum infection