title: Electrochemical deposition of mineralized BSA/collagen coating.
abstract: In this work, mineralized collagen coatings with different loading quantity of bovine serum albumin (BSA) were prepared via in situ electrochemical deposition on titanium substrate. The microstructure and BSA loading quantity of the coatings could be controlled by the electrochemical deposition parameters, such as deposition potential, BSA concentration and its adding sequence in the electrolyte. The BSA loading quantity in the coatings was obtained in the range of 0.0170-0.173mg/cm(2), enhancing the cell adhesion and proliferation of the coatings with the simultaneous release. The distinct release behaviors of BSA were attributed to their gradient distribution with different mineralization degrees, which could be adjusted by the deposition process. These results suggest that in situ electrochemical deposition is a promising way to incorporate functional molecules into the mineralized collagen coatings and the mineralized BSA/collagen coatings are highly promising for improving the rhBMP-2 loading capability (1.8-fold).
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China; The Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: wengwj@zju.edu.cn.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry*; D055664:Electrochemical Techniques; D004573:Electrolytes / Q000737:chemistry; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D014025:Titanium / Q000737:chemistry
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine; D003094:Collagen; D014025:Titanium
keywords: BSA; Cytocompatibility; Electrochemical deposition; Mineralized collagen coatings