title: Myo-Inositol content determined by myo-inositol biosynthesis and oxidation in blueberry fruit.
abstract: Myo-inositol metabolism in plant edible organs has become the focus of many recent studies because of its benefits to human health and unique functions in plant development. In this study, myo-inositol contents were analyzed during the development of two blueberry cultivars, cv 'Berkeley' and cv 'Bluecrop'. Furthermore, two VcMIPS 1/2 (Vaccinium corymbosum MIPS) genes, one VcIMP (Vaccinium corymbosum IMP) gene and one VcMIOX (Vaccinium corymbosum MIOX) gene were isolated for the first time from blueberry. The expression patterns of VcMIPS2, VcIMP and VcMIOX genes showed a relationship with the change profiles of myo-inositol content during fruit ripening. The results were further confirmed by the analyses of the enzyme activity. Results indicated that both myo-inositol biosynthesis and oxidation played important roles in determining of myo-inositol levels during the development of blueberry. To our knowledge, this report is the first to discuss myo-inositol levels in fruits in terms of biosynthesis and catabolism.
authors.affiliation: College of Life Sciences, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: songfangyuan1989@qq.com.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Song
authors.affiliation: College of Agriculture, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: suhongyan66@126.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Su
authors.affiliation: College of Life Sciences, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: Aligen@126.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Yang
authors.affiliation: College of Agriculture, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: zhu-luying@sohu.com.
authors.forename: Luying
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Agriculture, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: chengjieshan@163.com.
authors.forename: Jieshan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: College of Life Sciences, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: wanglei9909@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Agriculture, Ludong University, Yantai, Shandong 264025, PR China. Electronic address: chengxianhao@sohu.com.
authors.forename: Xianhao
authors.initials: X
authors.lastname: Cheng
mesh_terms: D029798:Blueberry Plants / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D007294:Inositol / Q000138:chemical synthesis*
chemical_list: D007294:Inositol
keywords: Blueberry; Fruit ripening; Gene expression; Myo-Inositol