title: Storage of Euschistus heros Eggs (Fabricius) (Hemiptera: Pentatomidae) in Liquid Nitrogen for Parasitization by Telenomus podisi Ashmead (Hymenoptera: Platygastridae).
abstract: Records in the literature with regard to the influence of freezing of pentatomid eggs on parasitism by microhymenopterans are scarce. In this research, we compared the storage of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) eggs in liquid nitrogen for different periods with the objective of optimizing the multiplication of Telenomus podisi Ashmead (Hymenoptera: Platygastridae) in the laboratory. Fresh eggs of E. heros were exposed (S3, S6) or not (NS3, NS6) to UV light for 30 min and stored in 1.5-mL plastic vials in liquid nitrogen either for 3 (S3, NS3) or 6 months (S6, NS6), and egg suitability to parasitoid development was compared to control eggs exposed (SC) or not (NSC) to UV treatment. Global data analysis showed that E. heros eggs stored in liquid nitrogen with or without UV treatment, for 3 or 6 months, were suitable for T. podisi parasitization.
authors.affiliation: Depto de Ciências Biológicas, Univ do Estado de Mato Grosso, Jardim Aeroporto, MT 358, Km 07, CEP 78300-000, Tangará da Serra, MT, Brasil. favettibruna@yahoo.com.br.
authors.forename: B M
authors.initials: BM
authors.lastname: Favetti
authors.affiliation: Depto de Ciências Biológicas, Univ do Estado de Mato Grosso, Jardim Aeroporto, MT 358, Km 07, CEP 78300-000, Tangará da Serra, MT, Brasil.
authors.forename: A R
authors.initials: AR
authors.lastname: Butnariu
authors.affiliation: Depto de Ciências Biológicas, Univ do Estado de Mato Grosso, Jardim Aeroporto, MT 358, Km 07, CEP 78300-000, Tangará da Serra, MT, Brasil.
authors.forename: A K
authors.initials: AK
authors.lastname: Doetzer
mesh_terms: D000818:Animals; D015925:Cryopreservation; D006430:Hemiptera / Q000469:parasitology*; D020062:Heteroptera; D006790:Host-Parasite Interactions; D006927:Hymenoptera / Q000472:pathogenicity*; D009584:Nitrogen; D010063:Ovum; D014863:Wasps
chemical_list: D009584:Nitrogen
keywords: Biological control; egg preservation; freezing; parasitoid; stink bug