title: The Classification of Sini Decoction Pattern in Traditional Chinese Medicine by Gene Expression Profiling.
abstract: We investigated the syndromes of the Sini decoction pattern (SDP), a common ZHENG in traditional Chinese medicine (TCM). The syndromes of SDP were correlated with various severe Yang deficiency related symptoms. To obtain a common profile for SDP, we distributed questionnaires to 300 senior clinical TCM practitioners. According to the survey, we concluded 2 sets of symptoms for SDP: (1) pulse feels deep or faint and (2) reversal cold of the extremities. Twenty-four individuals from Taipei City Hospital, Linsen Chinese Medicine Branch, Taiwan, were recruited. We extracted the total mRNA of peripheral blood mononuclear cells from the 24 individuals for microarray experiments. Twelve individuals (including 6 SDP patients and 6 non-SDP individuals) were used as the training set to identify biomarkers for distinguishing the SDP and non-SDP groups. The remaining 12 individuals were used as the test set. The test results indicated that the gene expression profiles of the identified biomarkers could effectively distinguish the 2 groups by adopting a hierarchical clustering algorithm. Our results suggest the feasibility of using the identified biomarkers in facilitating the diagnosis of TCM ZHENGs. Furthermore, the gene expression profiles of biomarker genes could provide a molecular explanation corresponding to the ZHENG of TCM.
authors.affiliation: Institute of Nano Engineering and Microsystems, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu 300, Taiwan; Classics Chinese Medicine Clinic, Taipei 104, Taiwan.
authors.forename: Hung-Tsu
authors.initials: HT
authors.lastname: Cheng
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Chaang-Ray
authors.initials: CR
authors.lastname: Chen
authors.affiliation: Department of Genome Medicine, College of Medicine and Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chia-Yang
authors.initials: CY
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Institute of Statistics, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Wun-Yi
authors.identifier: 0000-0002-3724-5410
authors.initials: WY
authors.lastname: Shu
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Ian C
authors.identifier: 0000-0003-2782-9997
authors.initials: IC
authors.lastname: Hsu