title: Cytotoxic Steroidal Glycosides from the Whole Plant of Calamus acanthophyllus.
abstract: A new steroidal glycoside, callaphylloside (1), together with seven known glycosides (2-8), was isolated from the whole plant of Calamus acanthophyllus. The structure of the new compound was elucidated by spectral data analyses and chemical transformations. Compounds 5 and 8 exhibited strong cytotoxic activity against four cancer cell lines (0.7 ≤ IC50 ≤ 3.4 µM). Evaluation of the structure-activity relationship among steroidal glycosides revealed that the structure of spirostanol with an α-L-rhamnopyranosyl linked to C-2 of the inner glucopyranosyl residue both play a critical role in the effects of these compounds on the cancer cell lines.
authors.affiliation: Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Hunsa
authors.initials: H
authors.lastname: Prawat
authors.affiliation: Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Chulabhorn
authors.initials: C
authors.lastname: Mahidol
authors.affiliation: Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Kaweetripob
authors.affiliation: Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Pakamas
authors.initials: P
authors.lastname: Intachote
authors.affiliation: Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Pisutjaroenpong
authors.affiliation: Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Somsak
authors.initials: S
authors.lastname: Ruchirawat
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D031081:Calamus / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010840:Phytosterols / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D010840:Phytosterols; D013256:Steroids; C000614937:callaphylloside