title: Neuroprotective Activity of (-)-Epigallocatechin Gallate against Lipopolysaccharide-Mediated Cytotoxicity.
abstract: Lipopolysaccharide- (LPS-) mediated systemic inflammation plays a critical role in neurodegenerative diseases. The present study was conducted to evaluate the protective effects of epigallocatechin gallate (EGCG), the major component in green tea, on LPS-mediated inflammation and neurotoxicity. LPS treatment of macrophages induced expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6). However, EGCG pretreatment of macrophages significantly inhibited LPS-mediated induction of these cytokines. In addition, EGCG significantly diminished LPS-induced inflammatory cytokines in the peripheral mononuclear blood cells (PBMCs). Supernatant from EGCG-pretreated and LPS-activated macrophage cultures was found to be less cytotoxic to neurons than that from non-EGCG-pretreated and LPS-activated macrophage cultures. Furthermore, EGCG treatment of neurons could inhibit LPS-induced production of reactive oxygen species (ROS). Thus EGCG represents a potent and useful neuroprotective agent for inflammation-mediated neurological disorders.
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, Wuhan 430071, China.
authors.forename: Jin-Biao
authors.initials: JB
authors.lastname: Liu
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, Wuhan 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: Yi-Zhong
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: Department of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, Wuhan 430071, China; Department of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USA; State Key Laboratory of Virology, Wuhan University, Wuhan 430071, China.
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Ho
authors.affiliation: Department of Pathology & Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: Jie-Liang
authors.initials: JL
authors.lastname: Li
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D007249:Inflammation / Q000517:prevention & control* / Q000628:therapy; D018836:Inflammation Mediators / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D009474:Neurons / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013662:Tea / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D003470:Culture Media; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D013662:Tea; D014409:Tumor Necrosis Factor-alpha; D002392:Catechin; C045651:epigallocatechin gallate