title: A Turn on ESIPT Probe for Rapid and Ratiometric Fluorogenic Detection of Hg(2+) and its Application in Live-Cell Imaging.
abstract: A probe based on 2-(2'-hydroxyphenyl) benzothiazole (HBT) and thiophosphate has been synthesized and used for the ratiometric detection of Hg(2+). The probe was designed in such a way that the excited state intramolecular proton transfer (ESIPT) of the HBT moiety get blocked. The probe exhibited a strong fluorescence enhancement upon addition of Hg(2+) while showing almost no response to other cations in CH3CN/HEPES buffer solution. The probe exhibited fast selectivity towards Hg(2+) and could be completed in 1 min. Fluorescence imaging experiments of Hg(2+) in living TE-1 cells demonstrated its value of practical applications in biological systems.
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. ljha3100@163.com.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China. ljha3100@163.com.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China. ljha3100@163.com.
authors.forename: Jihong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yin
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
authors.forename: Hongqi
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. zhangjff@sina.com.|Henan Cereal Quality and Safety Testing Key Laboratory, Zhengzhou, 450002, China. zhangjff@sina.com.|Laboratory of Quality & Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture, Zhengzhou, 450002, China. zhangjff@sina.com.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008628:Mercury / Q000032:analysis* / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010710:Phosphates / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D010710:Phosphates; D008628:Mercury; C005465:benzothiazole; C035638:thiophosphoric acid
keywords: Deprotection; ESIPT; Fluorescence; Hg2+; Thiophosphate