title: Potential role of reactive oxygen species and antioxidant genes in the regulation of peach fruit development and ripening.
abstract: The roles of reactive oxygen species (ROS) as both toxic by-products and as signaling molecules have been reported in fruit development and ripening. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) play important roles in balancing the induction and removal of ROS in plants, and are respectively encoded by families of closely homologous genes. In the present study, we investigated the roles of ROS and the above-mentioned antioxidant genes during the development and ripening of peach fruit. The experimental results indicated that O2(-) and H2O2 acted as potential signaling molecules in the middle stage of fruit development, and only H2O2 might function as a main toxic molecule to stimulate lipid peroxidation and oxidative stress in the late stage of fruit ripening. PpaCu/Zn-SODs were the most abundant members in the PpaSOD gene family and they expressed steadily in peach fruit development and ripening. Low temperature (4 °C) postponed and suppressed the climacteric peaks of respiration and ethylene, significantly enhanced the activities of CAT and GPX, and up-regulated the expression of PpaCAT1 and PpaGPX6 in the late stage of fruit ripening. PpaCAT1 and PpaGPX6 were two key genes in alleviating oxidative stress in the late stage of fruit ripening.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huan
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: An
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Jiangsu, 210095, PR China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Jiangsu, 210095, PR China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Ma
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China. Electronic address: yuzhifang@njau.edu.cn.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D019069:Cell Respiration / Q000235:genetics; D005030:Ethylenes / Q000378:metabolism; D005638:Fruit / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D000068239:Prunus persica / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005030:Ethylenes; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides; C036216:ethylene; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Catalase; Gene expression; Glutathione peroxidase; Low temperature; Oxidative stress; Superoxide dismutase