title: RBQ3 participates in multiple myeloma cell proliferation, adhesion and chemoresistance.
abstract: Cell adhesion mediated drug resistance (CAM-DR) is a major factor that impedes the effect of chemotherapy in multiple myeloma (MM). RBQ3, which is a RB-binding protein, played a crucial role in cell cycle process. Here, we reported that RBQ3 expression was increased gradually during the proliferation process of myeloma cells. Knocking down of RBQ3 resulted in cell cycle arrest in G1 phase and increased myeloma cells adherent to fibronectin or bone marrow stromal cells (BMSCs). Furthermore, silencing of RBQ3 reduced sensitivity to chemotherapeutic drugs in myeloma cell lines adherent to BMSCs and reduced two apoptotic marker proteins cleaved caspase-3 and cleaved PARP expression. Besides, we also found that RBQ3 participated in MAPK/ERK signal transduction pathway. In summary, these results may shed new insights into the role of RBQ3 in the development of multiple myeloma.
authors.affiliation: Department of Hematology, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Hematology, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China; Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Ding
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
authors.forename: Qiru
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Oncology, Affiliated Cancer Hospital of Nantong University, Nantong 226361, Jiangsu Province, People's Republic of China. Electronic address: xuxiaohongnantong@163.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D020935:MAP Kinase Signaling System; D009101:Multiple Myeloma / Q000473:pathology*; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C470552:RBBP5 protein, human
keywords: CAM-DR; Multiple myeloma; RBQ3