title: Transplantation of human amniotic mesenchymal stem cells promotes neurological recovery in an intracerebral hemorrhage rat model.
abstract: Human amniotic membrane mesenchymal stem cells (hAMSCs) have recently been suggested as ideal candidate stem cells for cell-based therapy. Many studies have reported the therapeutic effects of hAMSCs in numerous disease models. However, no studies have used hAMSCs to treat intracerebral hemorrhage (ICH). In the present study, we examined the therapeutic potential of hAMSCs in a rat model of ICH, and characterized the possible mechanisms of action. Adult male Wistar rats were subjected to ICH by intrastriatal injection of VII collagenase, and then were intracerebrally administered hAMSCs, fibroblasts, or phosphate-buffered saline (PBS) at 24 h after ICH. Compared with the fibroblasts and the PBS control, hAMSCs treatment significantly promoted neurological recovery, and reduced the numbers of ED1(+) activated microglia, as well as myeloperoxidase (MPO(+)), and caspase-3(+) cells in the brain injury model. In addition, hAMSCs treatment significantly increased the expression of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) in the injured brain, and promoted neurogenesis and angiogenesis, compared with the fibroblasts and the PBS control. The transplanted hAMSCs survived for at least 27 days and were negative for β-tubulin III and glial fibrillary acidic protein (GFAP). Taken together, the results suggest that hAMSCs treatment significantly promotes neurological recovery in rats after ICH. The mechanism of action could be mediated by inhibition of inflammation and apoptosis, increasing neurotrophic factor expression, and promotion of neurogenesis and angiogenesis. Thus, hAMSCs are candidate stem cells for the treatment of ICH.
authors.affiliation: Affiliated Bayi Brain Hospital, Military General Hospital of Beijing PLA, Southern Medical University, Beijing, China; Neurosurgery Institute of Beijing Military Region, Beijing, China.
authors.forename: Honglong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Affiliated Bayi Brain Hospital, Military General Hospital of Beijing PLA, Southern Medical University, Beijing, China; Neurosurgery Institute of Beijing Military Region, Beijing, China.
authors.forename: Hongri
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shi Jiazhuang, China.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Affiliated Bayi Brain Hospital, Military General Hospital of Beijing PLA, Southern Medical University, Beijing, China; Neurosurgery Institute of Beijing Military Region, Beijing, China. Electronic address: jzxuruxiang@163.com.
authors.forename: Ruxiang
authors.initials: R
authors.lastname: Xu
mesh_terms: D000650:Amnion / Q000166:cytology*; D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology*; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000503:physiopathology* / Q000628:therapy*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D018919:Neovascularization, Physiologic; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D017208:Rats, Wistar; D020127:Recovery of Function; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D042461:Vascular Endothelial Growth Factor A
keywords: Human amniotic; Intracerebral hemorrhage; Mesenchymal stem cells; Neurological recovery; Transplantation