title: Purification and characterization of chitinase showing antifungal and biodegradation properties obtained from Streptomyces anulatus CS242.
abstract: In an effort to identify a microbial enzyme that can be useful as a fungicide and biodegradation agent of chitinous wastes, a chitinase (Chi242) was purified from the culture supernatant of Streptomyces anulatus CS242 utilizing powder of shrimp shell wastes as a sole carbon source. It was purified employing ammonium sulfate precipitation and gel permeation chromatography techniques. The molecular weight of the purified chitinase was ~38 kDa by SDS-PAGE. The N-terminal amino acid sequence (A-P-G-A-P-G-T-G-A-L) showed close similarity to those of other Streptomyes chitinases. The purified enzyme displayed optimal activity at pH 6.0 and 50 °C respectively. It showed substantial thermal stability for 2 h at 30-60 °C, and exhibited broad pH stability in the range 5.0-13.0 for 48 h at 4 °C. Scanning electron microscopy confirmed the ability of this enzyme to adsorb onto solid shrimp bio-waste and to degrade chitin microfibers. Chi242 could proficiently convert colloidal chitin to N-acetyl glucosamine (GlcNAc) and N-acetyl chitobiose (GlcNAc)2 signifying that this enzyme is suitable for bioconversion of chitin waste. In addition, it exerted an effective antifungal activity towards fungal pathogen signifying its role as a biocontrol agent. Thus, a single microbial cell of Streptomyces anulatus CS242 justified its dual role.
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju, 501-759, Korea.|Gachon Institute of Pharmaceutical Science, Gachon University, Incheon, 406-840, Korea.
authors.forename: Poonam
authors.initials: P
authors.lastname: Mander
authors.affiliation: Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, 534-729, Korea.
authors.forename: Seung Sik
authors.initials: SS
authors.lastname: Cho
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.forename: Sandesh
authors.initials: S
authors.lastname: Panthi
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
authors.forename: Yoon Seok
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Gachon Institute of Pharmaceutical Science, Gachon University, Incheon, 406-840, Korea.
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju, 501-759, Korea. jcyu@chosun.ac.kr.
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects / Q000502:physiology; D001673:Biodegradation, Environmental; D002688:Chitinases / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity / Q000187:drug effects / Q000502:physiology
chemical_list: D000935:Antifungal Agents; D002688:Chitinases
keywords: Antifungal enzyme; Chitinase; Chitinous shrimp waste; Chitooligosaccharide; Streptomyces anulatus