title: Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections.
abstract: We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of atypical pathogensatypical pathogens or viruses between the bead-based suspension array and real-time PCR. This technology can play a significant role in screening patients with pneumonia.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China slyyywb@126.com.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Hong-Ru
authors.initials: HR
authors.lastname: Li
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Zhi-Dan
authors.initials: ZD
authors.lastname: Hua
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Lin
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Meng-Qing
authors.initials: MQ
authors.lastname: Lin
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Wen-Sen
authors.initials: WS
authors.lastname: Huang
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Huang
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Yu
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Neng-Luan
authors.initials: NL
authors.lastname: Xu
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Bao-Song
authors.initials: BS
authors.lastname: Xie
authors.affiliation: Department of Viral Diseases, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
authors.forename: Xiao-Na
authors.initials: XN
authors.lastname: Shen
authors.affiliation: Department of Viral Diseases, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Xie
authors.affiliation: Shanghai Tellgen Life Science Co. Ltd, Shanghai 201203,China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Viral Diseases, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Huang
authors.affiliation: Clinical Microbiology Laboratory, Fujian Provincial Hospital, Fuzhou 350001, China.
authors.forename: Yan-An
authors.initials: YA
authors.lastname: Wu
authors.affiliation: Clinical Microbiology Laboratory, Fujian Provincial Hospital, Fuzhou 350001, China.
authors.forename: Xin-Lan
authors.initials: XL
authors.lastname: Hu
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D003948:Diagnostic Techniques, Respiratory System; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral
keywords: Acute respiratory tract infections; bead-based suspension array; high-throughput screening; pathogen detection; unexplained pneumonia