title: Correlation of Deoxynivalenol Accumulation in Fusarium-Infected Winter and Spring Wheat Cultivars with Secondary Metabolites at Different Growth Stages.
abstract: Fusarium infection in wheat causes Fusarium head blight, resulting in yield losses and contamination of grains with trichothecenes. Some plant secondary metabolites inhibit accumulation of trichothecenes. Eighteen Fusarium infected wheat cultivars were harvested at five time points and analyzed for the trichothecene deoxynivalenol (DON) and 38 wheat secondary metabolites (benzoxazinoids, phenolic acids, carotenoids, and flavonoids). Multivariate analysis showed that harvest time strongly impacted the content of secondary metabolites, more distinctly for winter wheat than spring wheat. The benzoxazinoid 2-β-glucopyranoside-2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA-glc), α-tocopherol, and the flavonoids homoorientin and orientin were identified as potential inhibitors of DON accumulation. Several phenolic acids, lutein and β-carotene also affected DON accumulation, but the effect varied for the two wheat types. The results could form a basis for choosing wheat cultivars using metabolite profiling as a marker for selecting wheat cultivars with improved resistance against Fusarium head blight and accumulation of trichothecene toxins in wheat heads.
authors.affiliation: Department of Agroecology and ‡Department of Molecular Biology and Genetics, Aarhus University , Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Etzerodt
authors.affiliation: Department of Agroecology and ‡Department of Molecular Biology and Genetics, Aarhus University , Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Rene
authors.initials: R
authors.lastname: Gislum
authors.affiliation: Department of Agroecology and ‡Department of Molecular Biology and Genetics, Aarhus University , Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Bente B
authors.initials: BB
authors.lastname: Laursen
authors.affiliation: Department of Agroecology and ‡Department of Molecular Biology and Genetics, Aarhus University , Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Heinrichson
authors.affiliation: Department of Agroecology and ‡Department of Molecular Biology and Genetics, Aarhus University , Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Per L
authors.initials: PL
authors.lastname: Gregersen
authors.affiliation: Department of Agroecology and ‡Department of Molecular Biology and Genetics, Aarhus University , Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Lise N
authors.initials: LN
authors.lastname: Jørgensen
authors.affiliation: Department of Agroecology and ‡Department of Molecular Biology and Genetics, Aarhus University , Forsøgsvej 1, 4200 Slagelse, Denmark.
authors.forename: Inge S
authors.initials: IS
authors.lastname: Fomsgaard
mesh_terms: D005506:Food Contamination / Q000032:analysis; D005670:Fusarium / Q000378:metabolism*; D015394:Molecular Structure; D009183:Mycotoxins / Q000737:chemistry / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D012621:Seasons; D064210:Secondary Metabolism; D014255:Trichothecenes / Q000737:chemistry / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology*
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol
keywords: FHB resistance; benzoxazinoids; flavonoids; phenolic acids; principal component analysis; tocopherols; wheat secondary metabolites