title: Conditional Knockout in Mice Reveals the Critical Roles of Ppp2ca in Epidermis Development.
abstract: The epidermis is an important tissue in Homo sapines and other animals, and an abnormal epidermis will cause many diseases. Phosphatase 2A (PP2A) is an important serine and threonine phosphatase. The α isoform of the PP2A catalytic subunit (Ppp2ca gene encoding PP2Acα) is critical for cell proliferation, growth, metabolism and tumorigenesis. However, to date, no study has revealed its roles in epidermis development. To specifically investigate the roles of PP2Acα in epidermis development, we first generated Ppp2ca(flox/flox) transgenic mice, and conditionally knocked out Ppp2ca in the epidermis driven by Krt14-Cre. Our study showed that Ppp2ca(flox/flox); Krt14-Cre mice had significant hair loss. In addition, histological analyses showed that the morphogenesis and hair regeneration cycle of hair follicles were disrupted in these mice. Moreover, Ppp2ca(flox/flox); Krt14-Cre mice had smaller size, melanin deposition and hyperproliferation at the base of the claws. Accordingly, our study demonstrates that PP2Acα plays important roles in both hair follicle and epidermis development. Additionally, the Ppp2ca(flox/flox) mice generated in this study can serve as a useful transgene model to study the roles of PP2Acα in other developmental processes and diseases.
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China. fangchaonjmu@hotmail.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Fang
authors.affiliation: Translational Medicine Center, Yancheng No. 1 People's Hospital, Yancheng 224000, China. leilipaper@outlook.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China. jianminlilab@njmu.edu.cn.|Model Animal Research Center of Nanjing Medical University, Nanjing 210029, China. jianminlilab@njmu.edu.cn.|Key Laboratory of National Reproductive Medicine Department of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing 210029, China. jianminlilab@njmu.edu.cn.|Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University Nanjing, Nanjing 210029, China. jianminlilab@njmu.edu.cn.|Department of cell biology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China. jianminlilab@njmu.edu.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
mesh_terms: D000505:Alopecia / Q000662:veterinary; D000818:Animals; D004817:Epidermis / Q000254:growth & development* / Q000378:metabolism; D018859:Hair Follicle / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D023421:Models, Animal; D009024:Morphogenesis; D054648:Protein Phosphatase 2 / Q000235:genetics* / Q000378:metabolism*
chemical_list: C578496:PPP2CA protein, mouse; D054648:Protein Phosphatase 2
keywords: Krt14-Cre; Ppp2ca; conditional knockout; hair loss