title: MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development.
abstract: MicroRNAs (miRNAs) are endogenous, noncoding small RNAs that function as critical regulators of gene expression. In plants, miRNAs have shown their potential as regulators of growth, development, signal transduction, and stress tolerance. Although the miRNA-mediated regulation of several processes is known, the involvement of miRNAs in regulating secondary plant product biosynthesis is poorly understood. In this study, we functionally characterized Arabidopsis (Arabidopsis thaliana) miR858a, which putatively targets R2R3-MYB transcription factors involved in flavonoid biosynthesis. Overexpression of miR858a in Arabidopsis led to the down-regulation of several MYB transcription factors regulating flavonoid biosynthesis. In contrast to the robust growth and early flowering of miR858OX plants, reduction of plant growth and delayed flowering were observed in Arabidopsis transgenic lines expressing an artificial miRNA target mimic (MIM858). Genome-wide expression analysis using transgenic lines suggested that miR858a targets a number of regulatory factors that modulate the expression of downstream genes involved in plant development and hormonal and stress responses. Furthermore, higher expression of MYBs in MIM858 lines leads to redirection of the metabolic flux towards the synthesis of flavonoids at the cost of lignin synthesis. Altogether, our study has established the potential role of light-regulated miR858a in flavonoid biosynthesis and plant growth and development.
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, India (D.S., M.T., A.P., C.B., A.S., P.K.T.); andAcademy of Scientific and Innovative Research, Anusandhan Bhawan, New Delhi 110 001, India (D.S., C.B., P.K.T.).
authors.forename: Deepika
authors.initials: D
authors.lastname: Sharma
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, India (D.S., M.T., A.P., C.B., A.S., P.K.T.); andAcademy of Scientific and Innovative Research, Anusandhan Bhawan, New Delhi 110 001, India (D.S., C.B., P.K.T.).
authors.forename: Manish
authors.initials: M
authors.lastname: Tiwari
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, India (D.S., M.T., A.P., C.B., A.S., P.K.T.); andAcademy of Scientific and Innovative Research, Anusandhan Bhawan, New Delhi 110 001, India (D.S., C.B., P.K.T.).
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Pandey
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, India (D.S., M.T., A.P., C.B., A.S., P.K.T.); andAcademy of Scientific and Innovative Research, Anusandhan Bhawan, New Delhi 110 001, India (D.S., C.B., P.K.T.).
authors.forename: Chitra
authors.initials: C
authors.lastname: Bhatia
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, India (D.S., M.T., A.P., C.B., A.S., P.K.T.); andAcademy of Scientific and Innovative Research, Anusandhan Bhawan, New Delhi 110 001, India (D.S., C.B., P.K.T.).
authors.forename: Ashish
authors.initials: A
authors.lastname: Sharma
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, India (D.S., M.T., A.P., C.B., A.S., P.K.T.); andAcademy of Scientific and Innovative Research, Anusandhan Bhawan, New Delhi 110 001, India (D.S., C.B., P.K.T.) prabodht@nbri.res.in.
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D015536:Down-Regulation; D005419:Flavonoids / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D055432:Metabolomics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C482738:BOTRYTIS SUSCEPTIBLE1 protein, Arabidopsis; D005419:Flavonoids; D035683:MicroRNAs; C000602913:Mirn858 microRNA, Arabidopsis; D014157:Transcription Factors