title: Draft genome sequence of Bradyrhizobium sp. strain BR 3267, an elite strain recommended for cowpea inoculation in Brazil.
abstract: The strain BR 3267 is a nitrogen-fixing symbiotic bacteria isolated from soil of semi-arid area of Brazilian Northeast using cowpea as the trap plant. This strain is used as commercial inoculant for cowpea and presents high efficient in nitrogen fixation as consequence of its adaptation potential to semi-arid conditions. We report here the draft genome sequence of Bradyrhizobium sp. strain BR 3267, an elite bacterium used as inoculant for cowpea. Whole genome sequencing of BR 3267 using Illumina MiSeq sequencing technology has 55 scaffolds with a total genome size of 7,904,309bp and C+G 63%. Annotation was added by the RAST prokaryotic genome annotation service and has shown 7314 coding sequences and 52 RNA genes.
authors.affiliation: Embrapa Agrobiologia, Seropédica, Rio de Janeiro, RJ, Brazil.
authors.forename: Jean Luiz
authors.initials: JL
authors.lastname: Simões-Araújo
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Departamento de Solos, Seropédica, Rio de Janeiro, RJ, Brazil.
authors.forename: Jakson
authors.initials: J
authors.lastname: Leite
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Departamento de Ciências Ambientais, Seropédica, Rio de Janeiro, RJ, Brazil.
authors.forename: Samuel Ribeiro
authors.initials: SR
authors.lastname: Passos
authors.affiliation: Embrapa Agrobiologia, Seropédica, Rio de Janeiro, RJ, Brazil.
authors.forename: Gustavo Ribeiro
authors.initials: GR
authors.lastname: Xavier
authors.affiliation: Embrapa Agrobiologia, Seropédica, Rio de Janeiro, RJ, Brazil.
authors.forename: Norma Gouvêa
authors.initials: NG
authors.lastname: Rumjanek
authors.affiliation: Embrapa Agrobiologia, Seropédica, Rio de Janeiro, RJ, Brazil. Electronic address: jerri.zilli@embrapa.br.
authors.forename: Jerri Édson
authors.initials: JÉ
authors.lastname: Zilli
mesh_terms: D001482:Base Composition; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D016366:Open Reading Frames; D053204:Root Nodules, Plant / Q000382:microbiology; D013559:Symbiosis; D000070658:Vigna / Q000382:microbiology* / Q000502:physiology
keywords: Biological nitrogen fixation; Next generation sequencing; Nodulation; Semi-arid region