title: Co-cultivation of Aspergillus nidulans Recombinant Strains Produces an Enzymatic Cocktail as Alternative to Alkaline Sugarcane Bagasse Pretreatment.
abstract: Plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Replacing these methods with co-cultivated enzymatic cocktails is an alternative. Here we propose a pretreatment for sugarcane bagasse using a multi-enzymatic cocktail from the co-cultivation of four Aspergillus nidulans recombinant strains. The co-cultivation resulted in the simultaneous production of GH51 arabinofuranosidase (AbfA), GH11 endo-1,4-xylanase (XlnA), GH43 endo-1,5-arabinanase (AbnA) and GH12 xyloglucan specific endo-β-1,4-glucanase (XegA). This core set of recombinant enzymes was more efficient than the alternative alkaline method in maintaining the cellulose integrity and exposing this cellulose to the following saccharification process. Thermogravimetric and differential thermal analysis revealed residual byproducts on the alkali pretreated biomass, which were not found in the enzymatic pretreatment. Therefore, the enzymatic pretreatment was residue-free and seemed to be more efficient than the applied alkaline method, which makes it suitable for bioethanol production.
authors.affiliation: Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo São Paulo, Brazil.
authors.forename: Matheus S
authors.initials: MS
authors.lastname: Lima
authors.affiliation: Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas São Paulo, Brazil.
authors.forename: André R de L
authors.initials: AR
authors.lastname: Damasio
authors.affiliation: Department of Mechanical Engineering, University of São Paulo São Paulo, Brazil.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Crnkovic
authors.affiliation: Laboratory of Biopathology and Molecular Biology, Uberaba University Uberaba, Brazil.
authors.forename: Marcelo R
authors.initials: MR
authors.lastname: Pinto
authors.affiliation: Federal University of Campina Grande Cuite, Brazil.
authors.forename: Ana M
authors.initials: AM
authors.lastname: da Silva
authors.affiliation: Federal Institute of Education, Science and Technology of São Paulo São Paulo, Brazil.
authors.forename: Jean C R
authors.initials: JC
authors.lastname: da Silva
authors.affiliation: Department of Biotechnology, Engineering School of Lorena, University of São Paulo São Paulo, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Segato
authors.affiliation: Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São PauloSão Paulo, Brazil; Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São PauloSão Paulo, Brazil.
authors.forename: Rosymar C
authors.initials: RC
authors.lastname: de Lucas
authors.affiliation: Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo São Paulo, Brazil.
authors.forename: João A
authors.initials: JA
authors.lastname: Jorge
authors.affiliation: Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo São Paulo, Brazil.
authors.forename: Maria de L T de M
authors.initials: Mde L
authors.lastname: Polizeli
keywords: Aspergillus nidulans; NaOH pretreatment; co-cultivation; differential thermal analysis; enzymatic cocktail; enzymatic pretreatment; sugarcane bagasse; thermogravimetric analysis