title: Zirconium-metalloporphyrin frameworks as a three-in-one platform possessing oxygen nanocage, electron media, and bonding site for electrochemiluminescence protein kinase activity assay.
abstract: A Zr-based metal-organic framework with zinc tetrakis(carboxyphenyl)-porphyrin (ZnTCPP) groups (MOF-525-Zn) was utilized to develop a novel electrochemiluminescence (ECL) biosensor for highly sensitive protein kinase activity assay. In this work, in terms of ECL measurements and cyclic voltammetry, the cathodic ECL behaviors of MOF-525-Zn in aqueous media were thoroughly investigated for the first time. The photoelectric active groups ZnTCPP on the MOF-525-Zn frameworks could promote the generation of singlet oxygen ((1)O2) via a series of electrochemical and chemical reactions, resulting in a strong and stable red irradiation at 634 nm. Additionally, the surfactant tetraoctylammonium bromide (TOAB) further facilitated dissolved oxygen to interact with the active sites ZnTCPP of MOF-525-Zn. Furthermore, the inorganic Zr-O clusters of MOF-525-Zn were simultaneously served as the recognition sites of phosphate groups. And then, an ultrasensitive ECL sensor was proposed for protein kinase A (PKA) activity detection with a linear range from 0.01 to 20 U mL(-1) and a sensitive detection limit of 0.005 U mL(-1). This biosensor can also be applied for quantitative kinase inhibitor screening. Finally, it exhibits good performance with high stability and acceptable fabrication reproducibility, which provide a valuable strategy for clinic diagnostics and therapeutics.
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. danshan@njust.edu.cn danshan@yzu.edu.cn.
authors.forename: Guang-Yao
authors.initials: GY
authors.lastname: Zhang
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. danshan@njust.edu.cn danshan@yzu.edu.cn.
authors.forename: Chang
authors.initials: C
authors.lastname: Cai
authors.affiliation: Univ. Grenoble Alpes, Département de Chimie Moléculaire, UMR CNRS 5250, 570 rue de la Chimie, CS 40700, 38058 Grenoble cedex 9, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.affiliation: Institute of Optoelectronics & Nanomaterials, Jiangsu Key Laboratory of Advanced Micro & Nano Materials and Technology, College of Material Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Zeng
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. danshan@njust.edu.cn danshan@yzu.edu.cn.
authors.forename: Xue-Ji
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. danshan@njust.edu.cn danshan@yzu.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
mesh_terms: D015374:Biosensing Techniques; D004583:Electrons; D008665:Metalloporphyrins / Q000737:chemistry*; D010100:Oxygen; D011494:Protein Kinases / Q000378:metabolism*; D015203:Reproducibility of Results; D015040:Zirconium / Q000737:chemistry*
chemical_list: D008665:Metalloporphyrins; D015040:Zirconium; D011494:Protein Kinases; D010100:Oxygen