title: Regulatory Role of a Receptor-Like Kinase in Specifying Anther Cell Identity.
abstract: In flowering plants, sequential formation of anther cell types is a highly ordered process that is essential for successful meiosis and sexual reproduction. Differentiation of meristematic cells and cell-cell communication are proposed to coordinate anther development. Among the proposed mechanisms of cell fate specification are cell surface-localized Leu-rich repeat receptor-like kinases (LRR-RLKs) and their putative ligands. Here, we present the genetic and biochemical evidence that a rice (Oryza sativa) LRR-RLK, MSP1 (MULTIPLE SPOROCYTE1), interacts with its ligand OsTDL1A (TPD1-like 1A), specifying the cell identity of anther wall layers and microsporocytes. An in vitro assay indicates that the 21-amino acid peptide of OsTDL1A has a physical interaction with the LRR domain of MSP1. The ostdl1a msp1 double mutant showed the defect in lacking middle layers and tapetal cells and having an increased number of microsporocytes similar to the ostdl1a or msp1 single mutant, indicating the same pathway of OsTDL1A-MSP1 in regulating anther development. Genome-wide expression profiles showed the altered expression of genes encoding transcription factors, particularly basic helix-loop-helix and basic leucine zipper domain transcription factors in ostdl1a and msp1 Among these reduced expressed genes, one putatively encodes a TGA (TGACGTCA cis-element-binding protein) factor OsTGA10, and another one encodes a plant-specific CC-type glutaredoxin OsGrx_I1. OsTGA10 was shown to interact with OsGrx_I1, suggesting that OsTDL1A-MSP1 signaling specifies anther cell fate directly or indirectly affecting redox status. Collectively, these data point to a central role of the OsTDL1A-MSP1 signaling pathway in specifying somatic cell identity and suppressing overproliferation of archesporial cells in rice.
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (L.Y., X.Q., M.C., W.L., D.Z.); Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (Q.F., B.C.M.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (B.C.M.); Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaian 223300, China (W.L., D.Z.); and School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia (D.Z.).
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (L.Y., X.Q., M.C., W.L., D.Z.); Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (Q.F., B.C.M.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (B.C.M.); Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaian 223300, China (W.L., D.Z.); and School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia (D.Z.).
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (L.Y., X.Q., M.C., W.L., D.Z.); Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (Q.F., B.C.M.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (B.C.M.); Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaian 223300, China (W.L., D.Z.); and School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia (D.Z.).
authors.forename: Mingjiao
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (L.Y., X.Q., M.C., W.L., D.Z.); Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (Q.F., B.C.M.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (B.C.M.); Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaian 223300, China (W.L., D.Z.); and School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia (D.Z.).
authors.forename: Qili
authors.identifier: 0000-0002-0860-2175
authors.initials: Q
authors.lastname: Fei
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (L.Y., X.Q., M.C., W.L., D.Z.); Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (Q.F., B.C.M.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (B.C.M.); Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaian 223300, China (W.L., D.Z.); and School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia (D.Z.).
authors.forename: Blake C
authors.identifier: 0000-0003-3436-6097
authors.initials: BC
authors.lastname: Meyers
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (L.Y., X.Q., M.C., W.L., D.Z.); Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (Q.F., B.C.M.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (B.C.M.); Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaian 223300, China (W.L., D.Z.); and School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia (D.Z.).
authors.forename: Wanqi
authors.identifier: 0000-0002-9938-5793
authors.initials: W
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University and University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (L.Y., X.Q., M.C., W.L., D.Z.); Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711 (Q.F., B.C.M.); Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (B.C.M.); Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaian 223300, China (W.L., D.Z.); and School of Agriculture, Food, and Wine, University of Adelaide, South Australia 5064, Australia (D.Z.) zhangdb@sjtu.edu.cn.
authors.forename: Dabing
authors.identifier: 0000-0002-1764-2929
authors.initials: D
authors.lastname: Zhang
mesh_terms: D035264:Flowers / Q000166:cytology* / Q000201:enzymology; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000166:cytology* / Q000201:enzymology / Q000235:genetics; D056771:Ovule / Q000235:genetics / Q000254:growth & development; D010084:Oxidation-Reduction; D059828:Plant Cells / Q000201:enzymology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D011494:Protein Kinases