title: PLGA-based microparticles loaded with bacterial-synthesized prodigiosin for anticancer drug release: Effects of particle size on drug release kinetics and cell viability.
abstract: This paper presents the synthesis and physicochemical characterization of biodegradable poly (d,l-lactide-co-glycolide) (PLGA)-based microparticles that are loaded with bacterial-synthesized prodigiosin drug obtained from Serratia marcescens subsp. Marcescens bacteria for controlled anticancer drug delivery. The micron-sized particles were loaded with anticancer drugs [prodigiosin (PG) and paclitaxel (PTX) control] using a single-emulsion solvent evaporation technique. The encapsulation was done in the presence of PLGA (as a polymer matrix) and poly-(vinyl alcohol) (PVA) (as an emulsifier). The effects of processing conditions (on the particle size and morphology) are investigated along with the drug release kinetics and drug-loaded microparticle degradation kinetics. The localization and apoptosis induction by prodigiosin in breast cancer cells is also elucidated along with the reduction in cell viability due to prodigiosin release. The implication of this study is for the potential application of prodigiosin PLGA-loaded microparticles for controlled delivery of cancer drug and treatment to prevent the regrowth or locoregional recurrence, following surgical resection of triple negative breast tumor.
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Obayemi
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Department of Materials Science and Engineering, Kwara State University, Malete, Ilorin, Kwara State, Nigeria.
authors.forename: Y
authors.initials: Y
authors.lastname: Danyuo
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria.
authors.forename: S
authors.initials: S
authors.lastname: Dozie-Nwachukwu
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria.
authors.forename: O S
authors.initials: OS
authors.lastname: Odusanya
authors.affiliation: Department of Mechanical and Aerospace Engineering, Princeton University, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, USA; Department of Chemistry, Bronx Community College, New York, NY, USA.
authors.forename: N
authors.initials: N
authors.lastname: Anuku
authors.affiliation: Department of Mechanical and Aerospace Engineering, Princeton University, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, USA.
authors.forename: K
authors.initials: K
authors.lastname: Malatesta
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, USA.
authors.forename: W
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Biomedical Engineering, Rutgers University, Piscataway, USA; Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Uhrich
authors.affiliation: Department of Materials Science and Engineering, African University of Science and Technology (AUST), Abuja, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, USA. Electronic address: soboyejo@princeton.edu.
authors.forename: W O
authors.initials: WO
authors.lastname: Soboyejo
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011353:Prodigiosin / Q000737:chemistry* / Q000494:pharmacology; D012706:Serratia marcescens / Q000737:chemistry / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011353:Prodigiosin; D017239:Paclitaxel
keywords: Degradation; Drug release kinetics; Encapsulation efficiency; Microparticles; Paclitaxel; Poly (d,l-lactide-co-glycolide); Prodigiosin