title: Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution.
abstract: This study reports a fast and quantitative determination method for phenylalanine (Phe) and tyrosine (Tyr) in human plasma using on-chip pressure-driven liquid chromatography. A pillar array column with low-dispersion turns and a gradient elution system was used. The separation of fluorescent derivatives of Phe, Tyr, and other hydrophobic amino acids was successfully performed within 140 s. Under the optimized conditions, Phe and Tyr in human plasma were quantified. The developed method is promising for rapid diagnosis in the clinical field.
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.|Key Laboratory of Tropic Biological Resources, Minister of Education, College of Marine Science, Hainan University, Haikou, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Song
authors.affiliation: Major in Nano-Science and Nano-Engineering, Waseda University, Tokyo, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Takatsuki
authors.affiliation: Major in Nano-Science and Nano-Engineering, Waseda University, Tokyo, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sekiguchi
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Funatsu
authors.affiliation: Major in Nano-Science and Nano-Engineering, Waseda University, Tokyo, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shoji
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan. makotot@mol.f.u-tokyo.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunoda
mesh_terms: D006801:Humans; D056656:Lab-On-A-Chip Devices; D010649:Phenylalanine / Q000097:blood*; D014443:Tyrosine / Q000097:blood*
chemical_list: D014443:Tyrosine; D010649:Phenylalanine
keywords: 4-Fluoro-7-nitro-2,1,3-benzoxadiazole; Amino acids; Fluorescence; Microfluidics; Quantitative determination