title: Two Redundant Receptor-Like Cytoplasmic Kinases Function Downstream of Pattern Recognition Receptors to Regulate Activation of SA Biosynthesis.
abstract: Salicylic acid (SA) serves as a critical signaling molecule in plant defense. Two transcription factors, SARD1 and CBP60g, control SA biosynthesis through regulating pathogen-induced expression of Isochorismate Synthase1, which encodes a key enzyme for SA biosynthesis. Here, we report that Pattern-Triggered Immunity Compromised Receptor-like Cytoplasmic Kinase1 (PCRK1) and PCRK2 function as key regulators of SA biosynthesis. In the pcrk1 pcrk2 double mutant, pathogen-induced expression of SARD1, CBP60g, and ICS1 is greatly reduced. The pcrk1 pcrk2 double mutant, but neither of the single mutants, exhibits reduced accumulation of SA and enhanced disease susceptibility to bacterial pathogens. Both PCRK1 and PCRK2 interact with the pattern recognition receptor FLS2, and treatment with pathogen-associated molecular patterns leads to rapid phosphorylation of PCRK2. Our data suggest that PCRK1 and PCRK2 function downstream of pattern recognition receptor in a signal relay leading to the activation of SA biosynthesis.
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Tongjun
authors.identifier: 0000-0002-0263-3448
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Na
authors.initials: N
authors.lastname: Qu
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Junling
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Yu-Ti
authors.initials: YT
authors.lastname: Cheng
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.).
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 (Q.K., T.S., J.M., M.L., Y.-t.C., Q.Z., D.W., Z.Z., Y.Z.);National Institute of Biological Sciences, Beijing 102206, China (N.Q.); andCollege of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China (J.M.) yuelin.zhang@ubc.ca.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001665:Binding Sites; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D060467:Disease Resistance / Q000276:immunology; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D009154:Mutation / Q000235:genetics; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000378:metabolism; D010766:Phosphorylation; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D044224:Pseudomonas syringae / Q000502:physiology; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C000622658:PCRK2 protein, Arabidopsis; D000069452:Pathogen-Associated Molecular Pattern Molecules; D051192:Receptors, Pattern Recognition; C036483:T-DNA; D000255:Adenosine Triphosphate; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis; C000606408:PCRK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D020156:Salicylic Acid