title: Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species.
abstract: Oxidative stress after birth led us to localize reactive oxygen and nitrogen species (RONS) production in the developing rat brain. Brains were assessed a day prenatally and on postnatal days 1, 2, 4, 8, 14, 30, and 60. Oxidation of dihydroethidium detected superoxide; 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate revealed hydrogen peroxide; immunohistochemical proof of nitrotyrosine and carboxyethyllysine detected peroxynitrite formation and lipid peroxidation, respectively. Blue autofluorescence detected protein oxidation. The foetuses showed moderate RONS production, which changed cyclically during further development. The periods and sites of peak production of individual RONS differed, suggesting independent generation. On day 1, neuronal/glial RONS production decreased indicating that increased oxygen concentration after birth did not cause oxidative stress. Dramatic changes in the amount and the sites of RONS production occurred on day 4. Nitrotyrosine detection reached its maximum. Day 14 represented other vast alterations in RONS generation. Superoxide production in arachnoidal membrane reached its peak. From this day on, the internal elastic laminae of blood vessels revealed the blue autofluorescence. The adult animals produced moderate levels of superoxide; all other markers reached their minimum. There was a strong correlation between detection of nitrotyrosine and carboxyethyllysine probably caused by lipid peroxidation initiated with RONS.
authors.affiliation: Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, Krč, 14220 Prague 4, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Wilhelm
authors.affiliation: Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, Krč, 14220 Prague 4, Czech Republic.
authors.forename: Richard
authors.initials: R
authors.lastname: Vytášek
authors.affiliation: Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, Krč, 14220 Prague 4, Czech Republic; Department of Histology and Embryology, 2nd Faculty of Medicine, Charles University in Prague, Plzeňská 311/221, Motol, 15000 Prague 5, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Uhlík
authors.affiliation: Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, Krč, 14220 Prague 4, Czech Republic; Department of Histology and Embryology, 2nd Faculty of Medicine, Charles University in Prague, Plzeňská 311/221, Motol, 15000 Prague 5, Czech Republic.
authors.forename: Luděk
authors.initials: L
authors.lastname: Vajner
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D006031:Glycosylation; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017208:Rats, Wistar; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: C000612723:N(6)-carboxyethyllysine; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013481:Superoxides; D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D006861:Hydrogen Peroxide; D008239:Lysine