title: Graphene oxide decorated monolithic column as stationary phase for capillary electrochromatography.
abstract: In this work, GO bonded monolith (pAS-GO@PS-DVB) as the stationary phase for capillary electrochromatography was fabricated, which was achieved by a simple one-step in-situ copolymerization of styrene and vinylized GO in the presence of divinylbenzene as a cross-linker. GO functionalization was primarily completed using p-aminostyrene based on condensation reaction between amino and carboxyl groups. The characterization by infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction and scanning electron microscopy proved the covalent bonding of GO on the monolith. The average pore diameter via Barrett-Joyner-Halenda, specific surface area and pore volume via Brunauer-Emmett-Teller equation by nitrogen adsorption/desorption were determined to be 112.4nm, 485.8m(2)g(-1) and 1.4cm(3)g(-1), respectively. The pAS-GO@PS-DVB monolithic column gave effective separation for a wide range of aromatic compounds, which was based on hydrogen bonding and π-π interactions of GO with polar and/or non-polar organic compounds. The reproducibility in terms of the precisions of migration time, peak height and peak area was estimated below 6% using thiourea and other aromatic compounds. Furthermore, the differences of migration time, peak height and peak area between the first-week analysis and the forth-week analysis were less than 19%, indicating good stability of the proposed monolithic column in one month. The applicability of the pAS-GO@PS-DVB monolith was also demonstrated by baseline separation of three phenols and three anilines.
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036, China. Electronic address: ycwang@hznu.edu.cn.
authors.forename: Yuanchao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Heyong
authors.initials: H
authors.lastname: Cheng
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Yili
authors.initials: Y
authors.lastname: Shen
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000327:Adsorption; D000814:Aniline Compounds / Q000737:chemistry / Q000302:isolation & purification*; D053801:Capillary Electrochromatography / Q000379:methods*; D004573:Electrolytes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry* / Q000302:isolation & purification; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D056951:Photoelectron Spectroscopy; D011137:Polystyrenes / Q000737:chemistry; D016062:Porosity; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared; D013890:Thiourea / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000097:Acetonitriles; D000814:Aniline Compounds; D004573:Electrolytes; D010087:Oxides; D010636:Phenols; D011137:Polystyrenes; D006108:Graphite; D013890:Thiourea; C032159:acetonitrile
keywords: Capillary electrochromatography; Graphene oxide; Monolithic columns; Preparation performance