title: A novel nanocatalytic SERS detection of trace human chorionic gonadotropin using labeled-free Vitoria blue 4R as molecular probe.
abstract: In pH 7.4 Na2HPO4-NaH2PO4 buffer solution containing the peptide probes for human chorionic gonadotropin (hCG), silver nanoparticles (AgNPs) were aggregated to big AgNPs clusters that exhibited very weak catalytic effect on the gold nanoparticle reaction of H2O2-HAuCl4. When hCG was present in the peptide probe solution, the AgNPs did not aggregate and it had strong catalytic effect on the gold nanoparticle reaction with a strong resonance Rayleigh scattering (RRS) peak at 370nm and a strong surface enhanced Raman scattering (SERS) peak at 1615cm(-1) in the presence of molecular probe of Victoria blue 4R (VB4R). With the increase of the hCG concentration, the catalysis enhanced due to the nanocatalyst of AgNPs increasing, and the RRS intensity increased at 370nm. The increased RRS intensity was linear to the hCG concentration in 0.05-10ng/mL, with a linear regression equation of ΔI370nm=409.8C +294. And the SERS intensity at 1615cm(-1) increased linearly with the hCG concentration in the range of 0.05-20ng/mL, with a linear regression equation of ΔI1615cm-1=142C+134. Based on this, two new methods of nanocatalytic SERS and RRS were proposed for the determination of trace hCG.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Wen
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
authors.forename: Qingye
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China. Electronic address: ahliang2008@163.com.
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China. Electronic address: zljiang@mailbox.gxnu.edu.cn.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D006063:Chorionic Gonadotropin / Q000097:blood* / Q000652:urine*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015335:Molecular Probes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D006063:Chorionic Gonadotropin; D015335:Molecular Probes; D010455:Peptides; D012394:Rosaniline Dyes; C016183:Victoria blue 4R; D012834:Silver; D006861:Hydrogen Peroxide
keywords: Human chorionic gonadotropin; Nanocatalysis; Peptide probe; RRS; SERS