title: DNA Methylation Analysis of Human Tissue-Specific Connexin Genes.
abstract: Connexins are the structural proteins of gap junctions and their functioning as tumor suppressors is well known. Epigenetic modifications, such as methylation of connexin genes, play important roles in regulating gene expression. Over the past decade, several methods have been applied to characterize DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in human gastric tissues using methylation-specific PCR, bisulfite-specific PCR sequencing as well as MassArray techniques.
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan, 410013, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan, 410013, China. xucanxia2000@163.com.
authors.forename: Canxia
authors.initials: C
authors.lastname: Xu
mesh_terms: D018031:Connexin 43 / Q000378:metabolism*; D017630:Connexins / Q000378:metabolism*; D018899:CpG Islands / Q000235:genetics; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D017629:Gap Junctions / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013447:Sulfites / Q000737:chemistry; D000097002:Gap Junction beta-1 Protein
chemical_list: D018031:Connexin 43; D017630:Connexins; D004247:DNA; D013447:Sulfites; D000097002:Gap Junction beta-1 Protein; C500216:GJA1 protein, human; C042345:hydrogen sulfite
keywords: Bisulfite-specific PCR sequencing; Chronic atrophic gastritis; Connexin; DNA methylation; Dysplasia; Gap junction; Gastric carcinoma; Intestinal metaplasia; MassArray; Methylation-specific PCR; Non-atrophic gastritis; Normal gastric mucosa