title: Banana Transcription Factor MaERF11 Recruits Histone Deacetylase MaHDA1 and Represses the Expression of MaACO1 and Expansins during Fruit Ripening.
abstract: Phytohormone ethylene controls diverse developmental and physiological processes such as fruit ripening via modulation of ethylene signaling pathway. Our previous study identified that ETHYLENE RESPONSE FACTOR11 (MaERF11), a transcription factor in the ethylene signaling pathway, negatively regulates the ripening of banana, but the mechanism for the MaERF11-mediated transcriptional regulation remains largely unknown. Here we showed that MaERF11 has intrinsic transcriptional repression activity in planta. Electrophoretic mobility shift assay and chromatin immunoprecipitation analyses demonstrated that MaERF11 binds to promoters of three ripening-related Expansin genes, MaEXP2, MaEXP7 and MaEXP8, as well as an ethylene biosynthetic gene MaACO1, via the GCC-box motif. Furthermore, expression patterns of MaACO1, MaEXP2, MaEXP7, and MaEXP8 genes are correlated with the changes of histone H3 and H4 acetylation level during fruit ripening. Moreover, we found that MaERF11 physically interacts with a histone deacetylase, MaHDA1, which has histone deacetylase activity, and the interaction significantly strengthens the MaERF11-mediated transcriptional repression of MaACO1 and Expansins Taken together, these findings suggest that MaERF11 may recruit MaHDA1 to its target genes and repress their expression via histone deacetylation.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Yan-Chao
authors.initials: YC
authors.lastname: Han
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Jian-Fei
authors.initials: JF
authors.lastname: Kuang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Jian-Ye
authors.identifier: 0000-0002-8975-6941
authors.initials: JY
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Xun-Cheng
authors.identifier: 0000-0002-4098-5284
authors.initials: XC
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Yun-Yi
authors.initials: YY
authors.lastname: Xiao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Jun-Ning
authors.initials: JN
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.).
authors.forename: Ke-Qiang
authors.identifier: 0000-0002-5791-3594
authors.initials: KQ
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China (Y.-C.H., J.-F.K., J.-Y.C., Y.-Y.X., C.-C.F., J.-N.W., W.-J.L.); Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China (X.-C.L.); and Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.-Q.W.) wjlu@scau.edu.cn.
authors.forename: Wang-Jin
authors.identifier: 0000-0002-2373-7422
authors.initials: WJ
authors.lastname: Lu
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000378:metabolism; D001483:Base Sequence; D005638:Fruit / Q000235:genetics* / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D028521:Musa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones; D010940:Plant Proteins; D014157:Transcription Factors; C095825:expansin protein, plant; D006655:Histone Deacetylases