title: Streptomyces chitinivorans sp. nov., a chitinolytic strain isolated from estuarine lake sediment.
abstract: A novel actinobacterial strain RC1832T was isolated from the sediment of a fish dumping yard at Balugaon near Chilika Lake. The strain is halotolerant (15 % NaCl, w/v), alkali-tolerant (pH 7-10) and hydrolyzes chitin, starch, gelatin, cellulose, carboxymethyl cellulose, Tween 80, tributyrin, lecithin and casein. Apart from showing typical genus-specific morphological and chemotaxonomic features, the comparision and analysis of the near complete 16S rRNA gene sequence clearly revealed that the strain RC1832T represented a member of the genus Streptomyces. It exhibited the highest sequence similarities with the strains Streptomyces fenghuangensis GIMN4.003T (99.78 %), Streptomyces nanhaiensis DSM 41926T (99.07 %), Streptomyces radiopugnans R97T(98.71 %), Streptomyces atacamensis DSM 42065T (98.65 %) and Streptomyces barkulensis DSM 42082T (98.25 %). The DNA-DNA relatedness of strain RC 1832T with the closest phylogenetic neighbours S. fenghuangensis GIMN4.003T and S. nanhaiensis DSM 41926T were 20±2 % and 21±2 %, respectively. Thus, based on a range of phenotypic and genotypic properties, strain RC1832T was suggested to represent a novel species of the genus Streptomyces for which the name Streptomyces chitinivorans sp. nov. is proposed. The type strain is RC1832T (=JCM 30611=KCTC 29696).
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.|School of Law, KIIT University, Bhubaneswar, Odisha, 715024, India.
authors.forename: Lopamudra
authors.initials: L
authors.lastname: Ray
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors.forename: Samir Ranjan
authors.initials: SR
authors.lastname: Mishra
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors.forename: Ananta Narayan
authors.initials: AN
authors.lastname: Panda
authors.affiliation: Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769008, India.
authors.forename: Surajit
authors.initials: S
authors.lastname: Das
authors.affiliation: Wetland Research and Training Centre, Chilika Development Authority, Balugaon, Odisha, 751014, India.
authors.forename: Gurdeep
authors.initials: G
authors.lastname: Rastogi
authors.affiliation: School of Law, KIIT University, Bhubaneswar, Odisha, 715024, India.
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Pattanaik
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Adhya
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors.forename: Mrutyunjay
authors.initials: M
authors.lastname: Suar
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
authors.forename: Vishakha
authors.initials: V
authors.lastname: Raina
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D063366:Estuaries; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S