title: Suicide inactivation of covalent peroxidase-mimicking DNAzyme with hydrogen peroxide and its protection by a reductant substrate.
abstract: Recently a covalent peroxidase-mimicking DNAzyme (cPMDNAzyme) with the improved catalytic activity was prepared. Here we demonstrate that hydrogen peroxide, the oxidant substrate of cPMDNAzyme is an inactivating agent of this catalyst. Presence of the reductant substrate, 2,2'-azino-bis(3-ethylbenthothiazoline-6-sulfonic acid (ABTS) prevents the inactivation of cPMDNAzyme. The experimental conditions (pH-optimum, concentrations of ABTS and H2O2) for the determination of cPMDNAzyme activity were optimized that allows a construction of the colorimetric cPMDNAzyme-based biosensors and assays with improved sensitivity.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Leninskie gory, Moscow 119991, Russia.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Gribas
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Leninskie gory, Moscow 119991, Russia.
authors.forename: Timofey S
authors.initials: TS
authors.lastname: Zatsepin
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Leninskie gory, Moscow 119991, Russia.
authors.forename: Sergey P
authors.initials: SP
authors.lastname: Korolev
authors.affiliation: Belozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, Leninskie gory, Moscow 119991, Russia.
authors.forename: Marina B
authors.initials: MB
authors.lastname: Gottikh
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Leninskie gory, Moscow 119991, Russia. Electronic address: sakharovivan@gmail.com.
authors.forename: Ivan Yu
authors.initials: IY
authors.lastname: Sakharov
mesh_terms: D052160:Benzothiazoles / Q000378:metabolism* / Q000494:pharmacology; D055162:Biocatalysis; D040761:Biomimetic Materials / Q000378:metabolism*; D021881:DNA, Catalytic / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D019163:Reducing Agents / Q000378:metabolism* / Q000494:pharmacology; D013451:Sulfonic Acids / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D052160:Benzothiazoles; D021881:DNA, Catalytic; D019163:Reducing Agents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: ABTS; DNAzyme; Hydrogen peroxide; Inactivation; Peroxidase