title: Photodynamic action of protoporphyrin IX derivatives on Trichophyton rubrum.
abstract: BACKGROUND
  Dermatophytes are filamentous keratinophilic fungi. Trichophyton rubrum is a prevalent infectious agent in tineas and other skin diseases. Drug therapy is considered to be limited in the treatment of such infections, mainly due to low accessibility of the drug to the tissue attacked and development of antifungal resistance in these microorganisms. In this context, Photodynamic Therapy is presented as an alternative.
  
  
  OBJECTIVE
  Evaluate, in vitro, the photodynamic activity of four derivatives of Protoporphyrin IX by irradiation with LED 400 nm in T. rubrum.
  
  
  METHODS
  Assays were subjected to irradiation by twelve cycles of ten minutes at five minute intervals.
  
  
  RESULTS
  Photodynamic action appeared as effective with total elimination of UFCs from the second irradiation cycle.
  
  
  CONCLUSIONS
  Studies show that the photodynamic activity on Trichophyton rubrum relates to a suitable embodiment of the photosensitizer, which can be maximized by functionalization of peripheral groups of the porphyrinic ring.
authors.affiliation: Fundação Educacional de Fernandópolis, Fernandópolis, SP, Brazil.
authors.forename: Rogério Rodrigo
authors.initials: RR
authors.lastname: Ramos
authors.affiliation: Universidade Camilo Castelo Branco, Fernandópolis, SP, Brazil.
authors.forename: Dora Inês
authors.initials: DI
authors.lastname: Kozusny-Andreani
authors.affiliation: Universidade Camilo Castelo Branco, Fernandópolis, SP, Brazil.
authors.forename: Adjaci Uchôa
authors.initials: AU
authors.lastname: Fernandes
authors.affiliation: Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Mauricio da Silva
authors.initials: Mda S
authors.lastname: Baptista
mesh_terms: D000704:Analysis of Variance; D000935:Antifungal Agents / Q000494:pharmacology; D003883:Arthrodermataceae / Q000187:drug effects; D015169:Colony Count, Microbial; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins; D012016:Reference Values; D015203:Reproducibility of Results; D013997:Time Factors; D014005:Tinea / Q000188:drug therapy; D014249:Trichophyton / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D017319:Photosensitizing Agents; D011524:Protoporphyrins; C028025:protoporphyrin IX