title: Nanoshell-Enhanced Raman Spectroscopy on a Microplate for Staphylococcal Enterotoxin B Sensing.
abstract: A sensitive surface-enhanced Raman scattering (SERS) immunosensor based on the Au nanoparticle (Au NP) shell structure was developed to detect staphylococcal enterotoxin B (SEB) on a microplate. Au NPs modified with 4-nitrothiophenol (4-NTP) and coated with Ag shell of controlled thickness at 6.6 nm exhibited excellent SERS intensity and were used as signal reporters in the detection of SEB. The engaged 4-NTP allowed the significant electromagnetic enhancement between Au NPs and the Ag shell and prevented the dissociation of the Raman reporter. More importantly, 4-NTP-differentiated SERS signals between the sample and microplate. The SERS-based immunosensor had a limit of detection of 1.3 pg/mL SEB. Analysis of SEB-spiked milk samples revealed that the developed method had high accuracy. Therefore, the SERS-encoded Au@Ag core-shell structure-based immunosensor is promising for the detection of biotoxins, pathogens, and environmental pollutants.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Cereals & Oils Nutrition Research Group, Academy of Science & Technology of State Administration of Grain , Beijing 100037, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhu
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D004768:Enterotoxins / Q000032:analysis / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles; D057145:Nanoshells / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; D006046:Gold
keywords: Au@Ag; SEB; SERS; antibody; core−shell