title: Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.
abstract: The identification and characterization of new tomato (Solanum lycopersicum) mutants affected in fruit pigmentation and nutritional content can provide valuable insights into the underlying biology, as well as a source of new alleles for breeding programs. To date, all characterized pink-pigmented tomato fruit mutants appear to result from low SlMYB12 transcript levels in the fruit skin. Two new mutant lines displaying a pink fruit phenotype (pf1 and pf2) were characterized in this study. In the pf mutants, SlMYB12 transcripts accumulated to wild-type levels but exhibited the same truncation, which resulted in the absence of the essential MYB activation domain coding region. Allelism and complementation tests revealed that both pf mutants were allelic to the y locus and showed the same recessive null allele in homozygosis: Δy A set of molecular and metabolic effects, reminiscent of those observed in the Arabidopsis (Arabidopsis thaliana) myb11 myb12 myb111 triple mutant, were found in the tomato Δy mutants. To our knowledge, these have not been described previously, and our data support the idea of their being null mutants, in contrast to previously described transcriptional hypomorphic pink fruit lines. We detected a reduction in the expression of several flavonol glycosides and some associated glycosyl transferases. Transcriptome analysis further revealed that the effects of the pf mutations extended beyond the flavonoid pathway into the interface between primary and secondary metabolism. Finally, screening for Myb-binding sites in the candidate gene promoter sequences revealed that 141 of the 152 co-down-regulated genes may be direct targets of SlMYB12 regulation.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.).
authors.forename: Josefina-Patricia
authors.identifier: 0000-0002-1872-4061
authors.initials: JP
authors.lastname: Fernandez-Moreno
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.).
authors.forename: Oren
authors.identifier: 0000-0002-7096-8063
authors.initials: O
authors.lastname: Tzfadia
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.).
authors.forename: Javier
authors.initials: J
authors.lastname: Forment
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.).
authors.forename: Silvia
authors.initials: S
authors.lastname: Presa
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.).
authors.forename: Ilana
authors.initials: I
authors.lastname: Rogachev
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.).
authors.forename: Sagit
authors.identifier: 0000-0001-7102-3152
authors.initials: S
authors.lastname: Meir
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.).
authors.forename: Diego
authors.initials: D
authors.lastname: Orzaez
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.) asaph.aharoni@weizmann.ac.il agranell@ibmcp.upv.es.
authors.forename: Aspah
authors.initials: A
authors.lastname: Aharoni
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Universidad Politécnica de Valencia, CP 46022 Valencia, Spain (J.-P.F.-M., J.F., S.P., D.O., A.G.);VIB/Ghent University, Bioinformatics and Systems Biology, B-9052 Gent, Belgium (O.T.); andDepartment of Plant Sciences and the Environment, Weizmann Institute of Science, Rehovot 76100, Israel (I.R., S.M., A.A.) asaph.aharoni@weizmann.ac.il agranell@ibmcp.upv.es.
authors.forename: Antonio
authors.identifier: 0000-0003-4266-9581
authors.initials: A
authors.lastname: Granell
mesh_terms: D000483:Alleles; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000096:biosynthesis / Q000235:genetics; D044948:Flavonols / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods; D009154:Mutation; D010858:Pigmentation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D017423:Sequence Analysis, RNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D005419:Flavonoids; D044948:Flavonols; D010940:Plant Proteins; D014157:Transcription Factors