title: Examination of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System of a Murine Model.
abstract: West Nile virus (WNV) is a neurotropic virus that causes inflammation and neuronal loss in the Central Nervous System leading to encephalitis and death. In this chapter, detailed methods to detect WNV in the murine brain tissue by quantitative real-time polymerase chain reaction and viral plaque assays are described. Determination of WNV neuropathogenesis by Hematoxylin and Eosin staining and immunohistochemical procedures are provided. In addition, TUNEL assays to determine neuronal loss during WNV neuropathogenesis are discussed in detail. Collectively, the methods mentioned in this chapter provide an overview to understand neuroinvasion and neuropathogenesis in a murine model of WNV infection.
authors.affiliation: Center for Molecular Medicine, Department of Biological Sciences, Old Dominion University, Norfolk, VA, 23529-0266, USA. hsultana@odu.edu.
authors.forename: Hameeda
authors.initials: H
authors.lastname: Sultana
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D002470:Cell Survival; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000378:metabolism; D014901:West Nile Fever / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D014902:West Nile virus / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: Blood–brain barrier; Neuroinvasion; Neuronal death; Neuropathogenesis; West Nile virus