title: Identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves.
abstract: From 6 samples of tropically grown fruits and leaves, 10 lactic acid bacteria belonging Leuconostoc, Weissella, and Lactobacillus species were isolated and identified by 16S rRNA gene sequencing and (GTG)5 fingerprinting. Acidification kinetics determined from BHI broth cultures showed genus-related patterns. In particular, Weissella cibaria appeared to act as a potent acidifier. Tolerance of isolates to acid, oxidative, or salt stress was highly variable and strain dependent. Isolate S14 (Leuconostoc pseudomesenteroides) growth was not affected by the presence of 0.05% H2O2, while Lactobacillus spp. isolates (S17 and S29) were the most tolerant to pH 4.5. The growth of 4 isolates, S5 (Leuconostoc mesenteroides), S14 and S10 (Leuconostoc pseudomesenteroides), and S27 (W. cibaria), was not affected by 5% NaCl. Nutritional beneficial properties were examined through measurement of antioxidant activities of short-term fermented pineapple juice, such as LDL oxidation and polyphenol content, and through exopolysaccharide formation from sucrose. Two isolates, S14 and S27, increased the antioxidant capacity of pineapple juice. The robust capacity of W. cibaria and of Leuconostoc pseudomesenteroides for vegetable lactic fermentation aimed to ameliorate food nutritional and functional quality was highlighted.
authors.affiliation: a Université de La Réunion, UMR QualiSud, ESIROI, 2 rue J. Wetzell, Parc Technologique Universitaire, Sainte Clotilde, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Fessard
authors.affiliation: b Université de La Réunion, UMR DéTROI - Inserm U1188 Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme CYROI, Saint Denis de La Réunion, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bourdon
authors.affiliation: c Université de La Réunion, EA LCSNSA, rue René Cassin, Saint Denis de La Réunion, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Payet
authors.affiliation: a Université de La Réunion, UMR QualiSud, ESIROI, 2 rue J. Wetzell, Parc Technologique Universitaire, Sainte Clotilde, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Remize
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001937:Brassica / Q000382:microbiology; D029441:Carica / Q000382:microbiology; D005285:Fermentation; D005524:Food Technology; D005638:Fruit / Q000382:microbiology*; D006861:Hydrogen Peroxide; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism; D007934:Leuconostoc / Q000378:metabolism; D018551:Solanum lycopersicum / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013312:Stress, Physiological; D014329:Tropical Climate
chemical_list: D000975:Antioxidants; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D006861:Hydrogen Peroxide
keywords: Leuconostoc spp.; Weissella spp.; adaptation au stress; antioxidant; antioxydant; diversity; diversité; stress adaptation