title: Diversity of culturable Gram-negative bacteria isolated from irrigation water of two rice crop regions in Southern Brazil.
abstract: In this study, we assessed the diversity of Gram-negative bacteria found in water used for irrigation of rice crops in two growing areas of southern Brazil. Samples were collected from the main irrigation channel and field drain area. Twenty-two bacterial species were found in Cachoeirinha and 28 in Camaquã. In both areas, the most frequent bacterial families were Enterobacteriaceae and Aeromonadaceae. Differences in microbial diversity were observed in both study areas. Thirty-five Gram-negative species were identified; however, only 15 were common in both locations. In addition, there were found pathogenic and drug-resistant species, such as Acinetobacter sp., Brucella spp., and Chryseobacterium meningosepticum. This study demonstrates the existence of a number of pathogenic species in aquatic ecosystems analyzed in three consecutive crop years, especially water used for rice production.
authors.affiliation: Microbiology and Toxicology Laboratory, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.
authors.forename: Maria Helena Lima Ribeiro
authors.initials: MH
authors.lastname: Reche
authors.affiliation: Microbiology and Toxicology Laboratory, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.
authors.forename: Catiusca
authors.initials: C
authors.lastname: Reali
authors.affiliation: Microbiology and Toxicology Laboratory, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.
authors.forename: Michele
authors.initials: M
authors.lastname: Pittol
authors.affiliation: Microbiology and Toxicology Laboratory, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.
authors.forename: Danilo
authors.initials: D
authors.lastname: de Athayde Saul
authors.affiliation: Microbiology and Toxicology Laboratory, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Molecular Biology Laboratory, PPG, Universidade do Vale do Rio dos Sinos (UNISINOS), Av. Unisinos, 950 - Bairro Cristo Rei - CEP, 93.022-750, São Leopoldo, Brazil.
authors.forename: Vera Regina Mussoi
authors.initials: VR
authors.lastname: Macedo
authors.affiliation: Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil. valiati@unisinos.br.|Molecular Biology Laboratory, PPG, Universidade do Vale do Rio dos Sinos (UNISINOS), Av. Unisinos, 950 - Bairro Cristo Rei - CEP, 93.022-750, São Leopoldo, Brazil. valiati@unisinos.br.
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Valiati
authors.affiliation: Microbiology and Toxicology Laboratory, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Molecular Biology Laboratory, PPG, Universidade do Vale do Rio dos Sinos (UNISINOS), Av. Unisinos, 950 - Bairro Cristo Rei - CEP, 93.022-750, São Leopoldo, Brazil.
authors.forename: Vilmar
authors.initials: V
authors.lastname: Machado
authors.affiliation: Microbiology and Toxicology Laboratory, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.|Postgraduate Program in Biology, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, Brazil.
authors.forename: Lidia Mariana
authors.initials: LM
authors.lastname: Fiuza
mesh_terms: D057914:Agricultural Irrigation; D001938:Brazil; D018556:Crops, Agricultural / Q000254:growth & development* / Q000382:microbiology; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004784:Environmental Monitoring / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D012275:Oryza / Q000254:growth & development* / Q000382:microbiology; D014871:Water Microbiology
keywords: 16S rRNA gene; Crop management; Enterobacteriaceae; Pathogens; Water