title: Real-Time Bioluminescence Imaging of Nitroreductase in Mouse Model.
abstract: Nitroreductase (NTR) is an endogenous reductase overexpressed in hypoxic tumors; however, its precise detection in living cells and animals remains a considerable challenge. Herein, we developed three reaction-based probes and a related bioluminescence assay for the real-time NTR detection. The high sensitivity and selectivity of probe 3, combined with its remarkable potential of bioluminescence imaging, affords a valuable approach for in vivo imaging of NTR in a tumor model mouse.
authors.affiliation: Institute of Clinical Trials, West China Hospital, Sichuan University , Chengdu, Sichuan 610041, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Feng
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
authors.forename: Huateng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Laboratory of Anesthesiology and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu, Sichuan 610041, China.
authors.forename: Quankun
authors.initials: Q
authors.lastname: Deng
authors.affiliation: The Laboratory of Anesthesiology and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu, Sichuan 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: The Laboratory of Anesthesiology and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu, Sichuan 610041, China.
authors.forename: Linghui
authors.initials: L
authors.lastname: Yang
authors.affiliation: The Laboratory of Anesthesiology and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu, Sichuan 610041, China.
authors.forename: Guobo
authors.initials: G
authors.lastname: Li
authors.affiliation: The Laboratory of Anesthesiology and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu, Sichuan 610041, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
authors.forename: Lupei
authors.initials: L
authors.lastname: Du
authors.affiliation: The Laboratory of Anesthesiology and Critical Care Medicine, Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu, Sichuan 610041, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Ke
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
authors.forename: Minyong
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D004195:Disease Models, Animal; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D008163:Luminescent Measurements; D051379:Mice; D015394:Molecular Structure; D009601:Nitroreductases / Q000032:analysis* / Q000378:metabolism; D061848:Optical Imaging; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D009601:Nitroreductases