title: Urinary Proteomics Yield Pathological Insights for Ureteropelvic Junction Obstruction.
abstract: Prenatal hydronephrosis is a common condition that may spontaneously resolve after birth. However, this condition can result in renal damage and requires surgical correction in a number of cases. Preventing renal damage is paramount, but existing diagnostic technology is invasive, exposes infants to radiation, is costly, and is often indeterminate. A better understanding of the pathophysiology of renal obstruction as reflected in the urinary proteome may provide new insights into the disease that could potentially alter the clinical management of hydronephrosis. We performed a quantitative proteomics study of urine that was surgically obtained from eight clinically significant, unilaterally obstructed infants versus eight healthy controls, with the goal of identifying quantitatively varying proteins and the biological networks associated with them. Notably, urine was obtained from both the obstructed kidney and the bladder. Over 1100 proteins were identified, and a total of 76 quantitatively varying proteins were identified. Proteins involved in oxidative stress, inflammation, and renal disease pathways showed the most significant abundance differences. This study gives a deeper understanding of the critical proteomic changes associated with renal obstruction and represents the deepest proteomic profile of renal obstruction to date.
authors.affiliation: From the ‡Department of Urology and the Urological Diseases Research Center, §Proteomics Center at Children's Hospital Boston, Boston, MA.
authors.forename: John W
authors.initials: JW
authors.lastname: Froehlich
authors.affiliation: From the ‡Department of Urology and the Urological Diseases Research Center, §Proteomics Center at Children's Hospital Boston, Boston, MA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Kostel
authors.affiliation: From the ‡Department of Urology and the Urological Diseases Research Center, §Proteomics Center at Children's Hospital Boston, Boston, MA.
authors.forename: Patricia S
authors.initials: PS
authors.lastname: Cho
authors.affiliation: From the ‡Department of Urology and the Urological Diseases Research Center, §Proteomics Center at Children's Hospital Boston, Boston, MA.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Briscoe
authors.affiliation: §Proteomics Center at Children's Hospital Boston, Boston, MA ¶Department of Pathology, Children's Hospital Boston and Harvard Medical School, Boston, MA;
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.affiliation: From the ‡Department of Urology and the Urological Diseases Research Center, §Proteomics Center at Children's Hospital Boston, Boston, MA.
authors.forename: Ali R
authors.initials: AR
authors.lastname: Vaezzadeh
authors.affiliation: From the ‡Department of Urology and the Urological Diseases Research Center, §Proteomics Center at Children's Hospital Boston, Boston, MA richard.lee@childrens.harvard.edu.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007668:Kidney / Q000378:metabolism*; D008297:Male; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D014517:Ureteral Obstruction / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism*
chemical_list: D015415:Biomarkers