title: Antioxidant and Hepatoprotective Efficiency of Selenium Nanoparticles Against Acetaminophen-Induced Hepatic Damage.
abstract: Overdoses of acetaminophen (APAP), a famous and widely used drug, may have hepatotoxic effects. Nanoscience is a novel scientific discipline that provides specific tools for medical science problems including using nano trace elements in hepatic diseases. Our study aimed to assess the hepatoprotective role of selenium nanoparticles (Nano-Se) against APAP-induced hepatic injury. Twenty-four male rats were classified into three equal groups: a control group that received 0.9 % NaCl, an APAP-treated group (oral administration), and a group treated with Nano-Se (10-20 nm, intraperitoneal (i.p.) injection) and APAP (oral administration). APAP overdose induced significant elevations in liver function biomarkers, hepatic lipid peroxidation, hepatic catalase, and superoxide dismutase (SOD), decreased the reduced glutathione (GSH) content and glutathione reductase (GR) activity, and stimulated significant DNA damage in hepatocytes, compared to control rats. Nano-Se administration improved the hepatic antioxidant protection mechanism and decreased cellular sensitivity to DNA fragmentation. Nano-Se exhibits a protective effect against APAP-induced hepatotoxicity through improved liver function and oxidative stress mediated by catalase, SOD, and GSH and decreases hepatic DNA fragmentation, a hepatic biomarker of cell death. Nano-Se could be a novel hepatoprotective strategy to inhibit oxidative stress.
authors.affiliation: Department of Chemistry, College of Science, University of Dammam, PO Box 383, Dammam, 31113, Dammam, Saudi Arabia. kaothman@uod.edu.sa.|Biochemistry Department, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef City, Egypt. kaothman@uod.edu.sa.
authors.forename: Kamal Adel
authors.initials: KA
authors.lastname: Amin
authors.affiliation: Biochemistry Department, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef City, Egypt.
authors.forename: Khalid Shaban
authors.initials: KS
authors.lastname: Hashem
authors.affiliation: Department of Chemistry, College of Science, University of Dammam, PO Box 383, Dammam, 31113, Dammam, Saudi Arabia.
authors.forename: Fawziah Saleh
authors.initials: FS
authors.lastname: Alshehri
authors.affiliation: Biochemistry Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
authors.forename: Said T
authors.initials: ST
authors.lastname: Awad
authors.affiliation: Internal Medicine Department, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef City, Egypt.
authors.forename: Mohammed S
authors.initials: MS
authors.lastname: Hassan
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D000082:Acetaminophen; D012643:Selenium
keywords: APAP; Hepatotoxicity; Histopathology; Nano-Se; Oxidative stress