title: N-acetylcysteine supplementation reduces oxidative stress for cytosine arabinoside in rat model.
abstract: Cytosine arabinoside (ARA-C) is a pyrimidine analog that may cause keratoconjunctivitis when used in high doses. The underlying mechanism may be the increased amounts of reactive oxygen radicals that may damage the DNA synthesis of corneal and conjunctival epithelial cells. Topical corticosteroids are one of the prophylactic treatments for keratoconjunctivitis induced by ARA-C. Forty Wistar-type albino rats were included in this study the rats were divided into four groups. The first group (Group 1) received only ARA-C, the second group (Group 2) received ARA-C and N-acetylcysteine (NAC), the third group (Group 3) received only NAC and the fourth group (Group 4) was the control group. The total oxidant status (TOS), the total antioxidant capacity and the oxidative stress index (OSI) measurements of the cornea and the conjunctiva were evaluated in these four groups. The mean TOS and OSI value was the highest in Group 1 and the lowest in Group 3. The differences in TOS and OSI values were statistically significant between Group 1 and Group 2. There are decreases in TOS and OSI values in rats which received ARA-C with NAC administration. NAC may have a protective effect on ARA-C-induced keratoconjunctivitis.
authors.affiliation: Department of Pediatric Hematology and Oncology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey. dryibalci@gmail.com.
authors.forename: Yasemin Isik
authors.initials: YI
authors.lastname: Balci
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
authors.forename: Semra
authors.initials: S
authors.lastname: Acer
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Yagci
authors.affiliation: Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
authors.forename: Vural
authors.initials: V
authors.lastname: Kucukatay
authors.affiliation: Department of Pediatric Hematology and Oncology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Sarbay
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
authors.forename: Kerem
authors.initials: K
authors.lastname: Bozkurt
authors.affiliation: Department of Pediatric Hematology and Oncology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey.
authors.forename: Aziz
authors.initials: A
authors.lastname: Polat
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003228:Conjunctiva / Q000187:drug effects*; D003315:Cornea / Q000187:drug effects*; D003561:Cytarabine / Q000494:pharmacology*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D007166:Immunosuppressive Agents; D003561:Cytarabine; D000111:Acetylcysteine
keywords: Cytosine arabinoside; Keratoconjunctivitis; N-acetylcysteine