title: Orexins and receptor OX2R in the gastroenteric apparatus of two teleostean species: Dicentrarchus labrax and Carassius auratus.
abstract: Orexin A and B peptides and the receptor OX2R were studied in sea bass and goldfish gastroenteric tract by immunoblotting combined with densitometric analysis using NIH Image J software and immunohistochemical techniques. These teleost species present a different gut organization and diverse feeding habits. Immunoblotting experiments showed one band of 16 kDa corresponding to prepro-orexin, and one band of 38 kDa corresponding to the OX2R receptor. Immunohistochemical localization of OXA and OXB was observed in the enteric nervous system throughout the gastroenteric tract of both species. OXA and OXB immunoreactive cells were found in the gastric and intestinal regions of sea bass, and were mainly found in the basal region of folds in intestinal bulb, and in the midgut and hindgut of goldfish. The distribution of OX2R was mainly detected in the mucosa of the gastroenteric tract of sea bass and goldfish. This distribution suggests an endocrine action of OXA and OXB in the gastrointestinal tract as well as involvement in the peripheral control of food intake and digestive processes in both species. This study might also serve to determine the productive factors in breeding and as a baseline for future experimental studies on the regulation of the gastroenteric functions in non-mammalian vertebrates. Anat Rec, 299:1121-1129, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Italy.
authors.forename: Livia
authors.initials: L
authors.lastname: D'Angelo
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: Castaldo
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: de Girolamo
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Lucini
authors.affiliation: Department of Sciences and Technologies, University of Sannio, Benevento, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Paolucci
authors.affiliation: Department of Advanced Biomedical Sciences, University of Naples Federico II, Italy.|Institute of Biostructures and Bioimages, National Research Council, Naples, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pelagalli
authors.affiliation: Department of Sciences and Technologies, University of Sannio, Benevento, Italy.
authors.forename: Ettore
authors.initials: E
authors.lastname: Varricchio
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Arcamone
mesh_terms: D000818:Animals; D001492:Bass / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D005247:Feeding Behavior; D029941:Fish Proteins / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism*; D007150:Immunohistochemistry; D064446:Orexin Receptors / Q000378:metabolism*; D000068797:Orexins / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D064446:Orexin Receptors; D000068797:Orexins
keywords: Orexins; gastroenteric apparatus; goldfish; sea bass