title: Protection and immunological study on two tetraspanin-derived vaccine candidates against schistosomiasis japonicum.
abstract: Tetraspanins (TSPs) are proteins found on the surface of helminth parasites of the genus Schistosoma and are regarded as potentially protective antigens. The large extracellular loop of Schistosoma mansoni tetraspanin-2, Sm-TSP-2, when fused to a thioredoxin partner and formulated with Freund's adjuvants, has been shown to be an efficacious vaccine against murine schistosomiasis. It is well recognized that CD4(+) T-cell-dependent immunity might play an important role against schistosomes; however, the contribution of CD8(+) T cells against multicellular pathogen is still uncertain. The exogenous protein-pulsed dendritic cells (DCs) can easily activate CD4(+) T cells response, while CD8(+) T cells response was relatively difficult to be induced. In this study, we evaluated the immunogenicity of TSP2HD antigen (hydrophilic domain of the S. japonicum tetraspanin-2) and TAT (the protein transduction domain of HIV-1)-coupled TSP2HD protein. As TAT-fused protein could promote major histocompatibility complex class I-dependent antigen presentation in vitro, TAT-TSP2HD-pulsed DCs induced stronger proliferation of schistosome-specific CD8(+) T cells compared with DCs incubated with TSP2HD alone. Vaccination with TAT-TSP2HD-pulsed DCs in vivo could improve disease outcome in S. japonicum-infected mice and was slightly superior to vaccination with DCs treated with TSP2HD. In summary, these data showed that TAT fusion proteins could help activate CD8(+) cells and Th1 cells and provide part protection against schistosome.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.|Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.|Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.|Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: M
authors.initials: M
authors.lastname: Hou
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.|Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: B
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: X
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China. jiminjun@njmu.edu.cn.|Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China. jiminjun@njmu.edu.cn.
authors.forename: M
authors.initials: M
authors.lastname: Ji
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.|Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, China.
authors.forename: G
authors.initials: G
authors.lastname: Wu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D007155:Immunologic Factors / Q000276:immunology*; D051379:Mice; D012549:Schistosoma japonicum / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology; D012554:Schistosomiasis japonica / Q000469:parasitology / Q000517:prevention & control*; D059470:Tetraspanins / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D013879:Thioredoxins / Q000235:genetics / Q000276:immunology; D014612:Vaccines / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D007155:Immunologic Factors; C000589037:TSP-2 protein, Schistosoma mansoni; D059470:Tetraspanins; D014612:Vaccines; D013879:Thioredoxins
keywords: Schistosoma japonicum; dendritic cells; protein transduction domain; tetraspanin-2