title: Development of a real-time PCR assay for quantification of Citrobacter rodentium.
abstract: Molecular tools to quantify Citrobacter rodentium are not available. We developed a quantitative PCR assay targeting the espB gene. This assay is specific, has a linearity range of about 6.7×10(1) to 6.7×10(6)cells/PCR reaction (92% efficiency) and a detection limit of about 10(4)cells/g wet feces.
authors.affiliation: Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, ON, Canada.
authors.forename: Sofia
authors.initials: S
authors.lastname: Sagaidak
authors.affiliation: Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, ON, Canada.
authors.forename: Amel
authors.initials: A
authors.lastname: Taibi
authors.affiliation: Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, ON, Canada.
authors.forename: Bijun
authors.initials: B
authors.lastname: Wen
authors.affiliation: Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, ON, Canada; Centre for Child Nutrition and Health, Faculty of Medicine, University of Toronto, ON, Canada. Electronic address: elena.comelli@utoronto.ca.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Comelli
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000379:methods*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D044082:Citrobacter rodentium / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial; D005243:Feces / Q000382:microbiology*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial
keywords: Citrobacter rodentium; Mice; espB; qPCR