title: Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses.
abstract: Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.
authors.forename: Andy
authors.initials: A
authors.lastname: Kilianski
authors.forename: Pierce A
authors.initials: PA
authors.lastname: Roth
authors.forename: Alvin T
authors.initials: AT
authors.lastname: Liem
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Hill
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Willis
authors.forename: Rebecca D
authors.initials: RD
authors.lastname: Rossmaier
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Marinich
authors.forename: Michele N
authors.initials: MN
authors.lastname: Maughan
authors.forename: Mark A
authors.initials: MA
authors.lastname: Karavis
authors.forename: Jens H
authors.initials: JH
authors.lastname: Kuhn
authors.forename: Anna N
authors.initials: AN
authors.lastname: Honko
authors.forename: C Nicole
authors.initials: CN
authors.lastname: Rosenzweig
mesh_terms: D018076:DNA, Complementary / Q000235:genetics*; D029043:Ebolavirus / Q000302:isolation & purification*; D004666:Encephalitis Virus, Venezuelan Equine / Q000302:isolation & purification*; D058608:Nanopores; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012313:RNA / Q000235:genetics*; D012328:RNA Viruses / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D012313:RNA
keywords: Ebola virus; RNA virus; Venezuelan equine encephalitis virus; Zika virus; fieldable platform; genomics; nanopore sequencing; sequencing; viruses