title: Capture of dengue viruses using antibody-integrated graphite-encapsulated magnetic beads produced using gas plasma technology.
abstract: Despite significant advances in medicine, global health is threatened by emerging infectious diseases caused by a number of viruses. Dengue virus (DENV) is a mosquito‑borne virus, which can be transmitted to humans via mosquito vectors. Previously, the Ministry of Health, Labour and Welfare in Japan reported the country's first domestically acquired case of dengue fever for almost 70 years. To address this issue, it is important to develop novel technologies for the sensitive detection of DENV. The present study reported on the development of plasma-functionalized, graphite-encapsulated magnetic nanoparticles (GrMNPs) conjugated with anti-DENV antibody for DENV capture. Radiofrequency wave‑excited inductively‑coupled Ar and ammonia gas plasmas were used to introduce amino groups onto the surface of the GrMNPs. The GrMNPs were then conjugated with an antibody against DENV, and the antibody‑integrated magnetic beads were assessed for their ability to capture DENV. Beads incubated in a cell culture medium of DENV‑infected mosquito cells were separated from the supernatant by applying a magnetic field and were then washed. The adsorption of DENV serotypes 1‑4 onto the beads was confirmed using reverse transcription‑polymerase chain reaction, which detected the presence of DENV genomic RNA on the GrMNPs. The methodology described in the present study, which employed the plasma-functionalization of GrMNPs to enable antibody‑integration, represents a significant improvement in the detection of DENV.
authors.affiliation: Laboratory of Biometabolic Chemistry, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903‑0215, Japan.
authors.forename: Akikazu
authors.initials: A
authors.lastname: Sakudo
authors.affiliation: Department of Nanovision Technology, Graduate School of Science and Technology, Shizuoka University, Naka‑Ku, Hamamatsu 432‑8561, Japan.
authors.forename: Anchu
authors.initials: A
authors.lastname: Viswan
authors.affiliation: Department of Electrical and Electronic Engineering, Graduate School of Engineering, Shizuoka University, Naka‑Ku, Hamamatsu 432‑8561, Japan.
authors.forename: Han
authors.initials: H
authors.lastname: Chou
authors.affiliation: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565‑0871, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Sasaki
authors.affiliation: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565‑0871, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
authors.affiliation: Department of Nanovision Technology, Graduate School of Science and Technology, Shizuoka University, Naka‑Ku, Hamamatsu 432‑8561, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nagatsu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006108:Graphite; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D053758:Nanoparticles; D058626:Plasma Gases
chemical_list: D000914:Antibodies, Viral; D058626:Plasma Gases; D006108:Graphite