title: Molecular Characterization of Arabidopsis GAL4/UAS Enhancer Trap Lines Identifies Novel Cell-Type-Specific Promoters.
abstract: Cell-type-specific gene expression is essential to distinguish between the numerous cell types of multicellular organism. Therefore, cell-type-specific gene expression is tightly regulated and for most genes RNA transcription is the central point of control. Thus, transcriptional reporters are broadly used markers for cell identity. In Arabidopsis (Arabidopsis thaliana), a recognized standard for cell identities is a collection of GAL4/UAS enhancer trap lines. Yet, while greatly used, very few of them have been molecularly characterized. Here, we have selected a set of 21 frequently used GAL4/UAS enhancer trap lines for detailed characterization of expression pattern and genomic insertion position. We studied their embryonic and postembryonic expression domains and grouped them into three groups (early embryo development, late embryo development, and embryonic root apical meristem lines) based on their dominant expression. We show that some of the analyzed lines are expressed in a domain often broader than the one that is reported. Additionally, we present an overview of the location of the T-DNA inserts of all lines, with one exception. Finally, we demonstrate how the obtained information can be used for generating novel cell-type-specific marker lines and for genotyping enhancer trap lines. The knowledge could therefore support the extensive use of these valuable lines.
authors.affiliation: Laboratory of Biochemistry, Wageningen University, 6703HA Wageningen, The Netherlands.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Radoeva
authors.affiliation: Laboratory of Biochemistry, Wageningen University, 6703HA Wageningen, The Netherlands.
authors.forename: Colette A
authors.identifier: 0000-0002-6110-2209
authors.initials: CA
authors.lastname: Ten Hove
authors.affiliation: Laboratory of Biochemistry, Wageningen University, 6703HA Wageningen, The Netherlands.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Saiga
authors.affiliation: Laboratory of Biochemistry, Wageningen University, 6703HA Wageningen, The Netherlands dolf.weijers@wur.nl.
authors.forename: Dolf
authors.identifier: 0000-0003-4378-141X
authors.initials: D
authors.lastname: Weijers
mesh_terms: D017360:Arabidopsis / Q000196:embryology / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018519:Meristem / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D019076:Transgenes
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C036483:T-DNA; D014157:Transcription Factors; D049452:Green Fluorescent Proteins