title: TRPV1 is crucial for proinflammatory STAT3 signaling and thermoregulation-associated pathways in the brain during inflammation.
abstract: Transient receptor potential vanilloid receptor 1 (TRPV1) is a non-selective cation channel that is stimulated by heat (>43 °C), mechanical/osmotic stimuli, and low pH. The importance of TRPV1 in inflammatory responses has been demonstrated, whereas its participation in brains remains unclear. In the present study, the intracerebroventricular (icv) administration of the TRPV1 agonist resiniferatoxin (RTX) induced the activation of signal transducer and activator of transcription 3 (STAT3) in circumventricular organs (CVOs) and thermoregulation-associated brain regions with a similar patttern to the peripheral and icv administration of lipopolysaccharide (LPS). With the peripheral and icv LPS stimuli, STAT3 activation was significantly lower in Trpv1(-/-) mice than in Trpv1(+/+) mice. The icv administration of RTX induced transient hypothermia, whereas that of the TRPV1 antagonist capsazepine enhanced the magnitude and period of LPS-induced hyperthermia. These results indicate that TRPV1 is important for activating proinflammatory STAT3 signaling and thermoregulation-associated brain pathways in the brain.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Yoshida
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Furube
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mannari
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, (National Institute for Physiological Sciences), National Institute of Natural Sciences, Okazaki, Aichi 444-8787, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Takayama
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, (National Institute for Physiological Sciences), National Institute of Natural Sciences, Okazaki, Aichi 444-8787, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kittaka
authors.affiliation: Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, (National Institute for Physiological Sciences), National Institute of Natural Sciences, Okazaki, Aichi 444-8787, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tominaga
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Miyata
mesh_terms: D000818:Animals; D001833:Body Temperature Regulation; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D002211:Capsaicin / Q000008:administration & dosage / Q000031:analogs & derivatives; D004224:Diterpenes / Q000008:administration & dosage; D008070:Lipopolysaccharides / Q000008:administration & dosage; D051379:Mice; D018345:Mice, Knockout; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D050916:TRPV Cation Channels / Q000172:deficiency / Q000378:metabolism*
chemical_list: D004224:Diterpenes; D008070:Lipopolysaccharides; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C024353:resiniferatoxin; C071423:capsazepine; D002211:Capsaicin