title: Raised HIF1α during normoxia in high altitude pulmonary edema susceptible non-mountaineers.
abstract: High altitude pulmonary edema (HAPE) susceptibility is associated with EGLN1 polymorphisms, we hypothesized that HAPE-susceptible (HAPE-S, had HAPE episode in past) subjects may exhibit abnormal HIF1α levels in normoxic conditions. We measured HIF1α levels in HAPE-S and HAPE resistant (HAPE-R, no HAPE episode) individuals with similar pulmonary functions. Hemodynamic responses were also measured before and after normobaric hypoxia (Fi02 = 0.12 for 30 min duration at sea level) in both groups. . HIF1α was higher in HAPE-S (320.3 ± 267.5 vs 58.75 ± 33.88 pg/ml, P < 0.05) than HAPE-R, at baseline, despite no significant difference in baseline oxygen saturations (97.7 ± 1.7% and 98.8 ± 0.7). As expected, HAPE-S showed an exaggerated increase in pulmonary artery pressure (27.9 ± 6 vs 19.3 ± 3.7 mm Hg, P < 0.05) and a fall in peripheral oxygen saturation (66.9 ± 11.7 vs 78.7 ± 3.8%, P < 0.05), when exposed to hypoxia. HIF1α levels at baseline could accurately classify members of the two groups (AUC = 0.87). In a subset of the groups where hemoglobin fractions were additionally measured to understand the cause of elevated hypoxic response at baseline, two of four HAPE-S subjects showed reduced HbA. In conclusion, HIF 1 α levels during normoxia may represent an important marker for determination of HAPE susceptibility.
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Soree
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Rajinder K
authors.initials: RK
authors.lastname: Gupta
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Krishan
authors.initials: K
authors.lastname: Singh
authors.affiliation: CSIR Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007, India.
authors.forename: Koundinya
authors.initials: K
authors.lastname: Desiraju
authors.affiliation: CSIR Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Agrawal
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Vats
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Bharadwaj
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: T P
authors.initials: TP
authors.lastname: Baburaj
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Chaudhary
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Singh
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Saroj
authors.initials: S
authors.lastname: Verma
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Amir Chand
authors.initials: AC
authors.lastname: Bajaj
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi 110054, India.
authors.forename: Shashi Bala
authors.initials: SB
authors.lastname: Singh
mesh_terms: D000328:Adult; D000532:Altitude Sickness / Q000378:metabolism* / Q000503:physiopathology*; D062186:Arterial Pressure; D015415:Biomarkers / Q000378:metabolism; D004198:Disease Susceptibility; D005260:Female; D006439:Hemodynamics; D006441:Hemoglobin A / Q000378:metabolism; D006801:Humans; D006976:Hypertension, Pulmonary / Q000378:metabolism* / Q000503:physiopathology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism; D012129:Respiratory Function Tests; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D006441:Hemoglobin A; D010100:Oxygen