title: Impact of insulin concentration and mode of FSH addition on the in vitro survival and development of isolated bovine preantral follicles.
abstract: The insulin and FSH are two important substances in the folliculogenesis process. Thus, the hypothesis of this experiment is that insulin concentration and the form of FSH addition affect the in vitro survival, growth, and estradiol production after culture of isolated bovine preantral follicles. The effects of insulin concentration (experiment 1) and the influence of both fixed and sequential concentrations of FSH (experiment 2) on the in vitro survival and development of bovine preantral follicles were investigated in this study by IVC for 18 days. In experiment 1, on Day 18 of culture, the addition of insulin at all concentrations promoted follicular survival rates significantly higher than that of the control, with the 10-ng/mL insulin treatment showing values significantly higher than the other treatments. The addition of 5- and 10-ng/mL insulin promoted higher follicular growth than the control and other treatments. In experiment 2, FSH 100 had a higher percentage of follicular viability compared with the control. FSH 100 produced follicle diameters significantly higher than those of the control and FSH seq.
  
  
  METHODS
  Estradiol levels in the presence of FSH (fixed concentration) were significantly higher than the other treatments. In conclusion, the association of insulin (10 ng/mL) and fixed concentration FSH (100 ng/mL) provides high rates of survival, growth, and estradiol production in bovine preantral follicles.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Rossetto
authors.affiliation: Biotechnology Nucleus of Sobral, Federal University of Ceara, Sobral, CE, Brazil.
authors.forename: M V A
authors.initials: MVA
authors.lastname: Saraiva
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: M P
authors.initials: MP
authors.lastname: Bernuci
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil.
authors.forename: G M
authors.initials: GM
authors.lastname: Silva
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil.
authors.forename: I R
authors.initials: IR
authors.lastname: Brito
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil.
authors.forename: A M C V
authors.initials: AMCV
authors.lastname: Alves
authors.affiliation: Postgraduate Biotechnology, Potiguar University/Laureate International Universities, Natal, RN, Brazil. Electronic address: dmmvet@hotmail.com.
authors.forename: D M
authors.initials: DM
authors.lastname: Magalhães-Padilha
authors.affiliation: Laboratory of Electron Microscopy, Department of Cell Biology, University of Brasilia, Brasilia, DF, Brazil.
authors.forename: S N
authors.initials: SN
authors.lastname: Báo
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil.
authors.forename: A P R
authors.initials: APR
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects*; D011374:Progesterone / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000662:veterinary*
chemical_list: D003470:Culture Media; D007328:Insulin; D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone
keywords: Bovine; FSH; Insulin; In vitro culture; Preantral follicle