title: Growth Hormone-Releasing Peptide 6 Enhances the Healing Process and Improves the Esthetic Outcome of the Wounds.
abstract: In addition to its cytoprotective effects, growth hormone-releasing peptide 6 (GHRP-6) proved to reduce liver fibrotic induration. CD36 as one of the GHRP-6 receptors appears abundantly represented in cutaneous wounds granulation tissue. The healing response in a scenario of CD36 agonistic stimulation had not been previously investigated. Excisional full-thickness wounds (6 mmØ) were created in the dorsum of Wistar rats and topically treated twice a day for 5 days. The universal model of rabbit's ears hypertrophic scars was implemented and the animals were treated daily for 30 days. Treatments for both species were based on a CMC jelly composition containing GHRP-6 400 μg/mL. Wounds response characterization included closure dynamic, RT-PCR transcriptional profile, histology, and histomorphometric procedures. The rats experiment indicated that GHRP-6 pharmacodynamics involves attenuation of immunoinflammatory mediators, their effector cells, and the reduction of the expression of fibrotic cytokines. Importantly, in the hypertrophic scars rabbit's model, GHRP-6 intervention dramatically reduced the onset of exuberant scars by activating PPARγ and reducing the expression of fibrogenic cytokines. GHRP-6 showed no effect on the reversion of consolidated lesions. This evidence supports the notion that CD36 is an active and pharmacologically approachable receptor to attenuate wound inflammation and accelerate its closure so as to improve wound esthetic.
authors.affiliation: Wound Healing and Cytoprotection Group, Biomedical Research Direction, Center for Genetic Engineering and Biotechnology, Avenue 31/158 and 190, P.O. Box 6162, Playa, 10600 Havana, Cuba.
authors.forename: Yssel
authors.initials: Y
authors.lastname: Mendoza Marí
authors.affiliation: Wound Healing and Cytoprotection Group, Biomedical Research Direction, Center for Genetic Engineering and Biotechnology, Avenue 31/158 and 190, P.O. Box 6162, Playa, 10600 Havana, Cuba.
authors.forename: Maday
authors.initials: M
authors.lastname: Fernández Mayola
authors.affiliation: Formulation Department, Technological Development Direction, Center for Genetic Engineering and Biotechnology, Avenue 31/158 and 190, P.O. Box 6162, Playa, 10600 Havana, Cuba.
authors.forename: Ana
authors.initials: A
authors.lastname: Aguilera Barreto
authors.affiliation: Wound Healing and Cytoprotection Group, Biomedical Research Direction, Center for Genetic Engineering and Biotechnology, Avenue 31/158 and 190, P.O. Box 6162, Playa, 10600 Havana, Cuba.
authors.forename: Ariana
authors.initials: A
authors.lastname: García Ojalvo
authors.affiliation: Formulation Department, Technological Development Direction, Center for Genetic Engineering and Biotechnology, Avenue 31/158 and 190, P.O. Box 6162, Playa, 10600 Havana, Cuba.
authors.forename: Yilian
authors.initials: Y
authors.lastname: Bermúdez Alvarez
authors.affiliation: Esthetic Surgery Department, "Joaquín Albarrán" Hospital, Avenue 26 and Boyeros, Plaza de la Revolución, 10600 Havana, Cuba.
authors.forename: Ana Janet
authors.initials: AJ
authors.lastname: Mir Benítez
authors.affiliation: Wound Healing and Cytoprotection Group, Biomedical Research Direction, Center for Genetic Engineering and Biotechnology, Avenue 31/158 and 190, P.O. Box 6162, Playa, 10600 Havana, Cuba.
authors.forename: Jorge
authors.initials: J
authors.lastname: Berlanga Acosta