title: OsGatB, the Subunit of tRNA-Dependent Amidotransferase, Is Required for Primary Root Development in Rice.
abstract: A short-root rice mutant was isolated from an ethyl methane sulfonate-mutagenized library. From map-based cloning strategy, a point mutation, resulting in an amino acid change from proline to leucine, was identified in the fourth exon of a glutamyl-tRNA (Gln) amidotransferase B subunit family protein (OsGatB, LOC_Os11g34210). This gene is an ortholog of Arabidopsis GatB and yeast PET112. GatB is a subunit of tRNA-dependent amidotransferase (AdT), an essential enzyme involved in Gln-tRNA(Gln) synthesis in mitochondria. Although previous studies have described that cessation in mitochondrial translation is lethal at very early developmental stages in plants, this point mutation resulted in a non-lethal phenotype of smaller root meristem and shorter root cell length. In the root, OsGatB was predominantly expressed in the root tip and played an important role in cell division and elongation there. OsGatB was localized in the mitochondria, and mitochondrial structure and function were all affected in Osgatb root tip cells.
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Linming
authors.initials: L
authors.lastname: Cheng
authors.affiliation: The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University Hangzhou, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: He
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Jingqin
authors.initials: J
authors.lastname: Shen
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University Hangzhou, China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University Hangzhou, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
keywords: OsGatB; Primary root; Rice; tRNA-dependent amidotransferase; tRNAGln