title: Profiles of gonadotropin-inhibitory hormone and melatonin during the sex change and maturation of cinnamon clownfish, Amphiprion melanopus.
abstract: The present study aimed to determine the relationship between melatonin and gonadotropin-inhibitory hormone (GnIH) and their effect on reproduction in cinnamon clownfish, Amphiprion melanopus. Accordingly, we investigated the expression pattern of GnIH, GnIH receptor (GnIH-R), and melatonin receptor (MT-R1) mRNA and protein, as well as the plasma levels of melatonin, during sex change in cinnamon clownfish. We found that GnIH and MT-R1 mRNA and melatonin activity were higher in fish with mature brain than in fish with developing gonads, and using double immunofluorescence staining, we found that both GnIH and MT-R1 proteins were co-expressed in the hypothalamus of cinnamon clownfish. These findings support the hypothesis that melatonin plays an important role in the negative regulation of maturation and GnIH regulation during reproduction.
authors.affiliation: Division of Marine BioScience, Korea Maritime and Ocean University, Busan, 49112, Republic of Korea.
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive N.W. Calgary, Alberta, T3B 2V4, Canada.
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Habibi
authors.affiliation: Division of Marine BioScience, Korea Maritime and Ocean University, Busan, 49112, Republic of Korea. Electronic address: choic@kmou.ac.kr.
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
mesh_terms: D000818:Animals; D005260:Female; D029941:Fish Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007028:Hypothalamic Hormones / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007031:Hypothalamus / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000032:analysis / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D010473:Perciformes / Q000097:blood / Q000254:growth & development* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D044094:Receptors, Melatonin / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012726:Sexual Behavior, Animal; D046468:Sexual Development
chemical_list: D029941:Fish Proteins; D007028:Hypothalamic Hormones; D012333:RNA, Messenger; D044094:Receptors, Melatonin; D008550:Melatonin
keywords: Amphiprion melanopus; Brain; GnIH; Maturation; Melatonin; Sex change