title: Brucellosis in Occupationally Exposed Groups.
abstract: BACKGROUND
  In India, high incidence of human brucellosis may be expected, as the conditions conducive for human brucellosis exist. Limited studies have been undertaken on human brucellosis especially in occupationally-exposed groups.
  
  
  OBJECTIVE
  To estimate prevalence of anti-brucellar antibodies, evaluate the clinical manifestations, risk factors and Knowledge, Attitude and Practices (KAP) levels about brucellosis among occupationally exposed groups.
  
  
  METHODS
  Blood samples were collected from 2337 occupationally exposed individuals. The serum samples were screened for the presence of anti-brucellar antibodies by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and 2-Mercaptoethanol test (2-ME). Clinical manifestations, risk factors and KAP levels were evaluated by personal interview using a structured questionnaire.
  
  
  RESULTS
  Seroprevalence of brucellosis by RBPT, SAT and 2-ME test was 9.46%, 4.45% and 3.64 % respectively. Clinical symptoms resembling brucellosis were seen in 91 subjects. The major risk factors were animal exposure in veterinarians and abattoirs, both animal exposure and raw milk ingestion in farmers and shepherds, exposure to raw milk and its ingestion in dairy workers and exposure to Brucella culture in laboratory workers. Except laboratory workers, few veterinarians and dairy workers none had heard about brucellosis. KAP levels regarding brucellosis were too poor in all the groups except laboratory workers.
  
  
  CONCLUSIONS
  Brucellosis most of the times was missed or misdiagnosed. Regular screenings for brucellosis and awareness programmes to increase KAP levels are necessary to control brucellosis in occupationally exposed groups.
authors.affiliation: Assistant Professor, Department of Microbiology, BLDEU's Shri B M Patil Medical College , Bijapur, Karnataka, India .
authors.forename: Smita S
authors.initials: SS
authors.lastname: Mangalgi
authors.affiliation: Professor, Department of Microbiology, BLDEU's Shri B M Patil Medical College , Bijapur, Karnataka, India .
authors.forename: Annapurna G
authors.initials: AG
authors.lastname: Sajjan
authors.affiliation: Professor, Department of Microbiology, Krishna Institute of Medical Sciences , Karad, Maharashtra, India .
authors.forename: Shivajirao T
authors.initials: ST
authors.lastname: Mohite
authors.affiliation: Assistant Professor, Department of Microbiology, BLDEU's Shri B M Patil Medical College , Bijapur, Karnataka, India .
authors.forename: Shivali
authors.initials: S
authors.lastname: Gajul
keywords: 2-ME; High-risk group; KAP; SAT; Zoonosis