title: Proteomic analysis of halotolerant proteins under high and low salt stress in Dunaliella salina using two-dimensional differential in-gel electrophoresis.
abstract: Dunaliella salina, a single-celled marine alga with extreme salt tolerance, is an important model organism for studying fundamental extremophile survival mechanisms and their potential practical applications. In this study, two-dimensional differential in-gel electrophoresis (2D-DIGE) was used to investigate the expression of halotolerant proteins under high (3 M NaCl) and low (0.75 M NaCl) salt concentrations. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and bioinformatics were used to identify and characterize the differences among proteins. 2D-DIGE analysis revealed 141 protein spots that were significantly differentially expressed between the two salinities. Twenty-four differentially expressed protein spots were successfully identified by MALDI-TOF/TOF MS, including proteins in the following important categories: molecular chaperones, proteins involved in photosynthesis, proteins involved in respiration and proteins involved in amino acid synthesis. Expression levels of these proteins changed in response to the stress conditions, which suggests that they may be involved in the maintenance of intracellular osmotic pressure, cellular stress responses, physiological changes in metabolism, continuation of photosynthetic activity and other aspects of salt stress. The findings of this study enhance our understanding of the function and mechanisms of various proteins in salt stress.
authors.affiliation: Pharmacy College, Xinxiang Medical University, Xinxiang 453003, Henan, China.|Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Jia
authors.affiliation: Pharmacy College, Xinxiang Medical University, Xinxiang 453003, Henan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Pharmacy College, Xinxiang Medical University, Xinxiang 453003, Henan, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Gao
authors.affiliation: Pharmacy College, Xinxiang Medical University, Xinxiang 453003, Henan, China.
authors.forename: Xi-Cheng
authors.initials: XC
authors.lastname: Wang
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China.
authors.forename: Le-Le
authors.initials: LL
authors.lastname: Qiu
authors.affiliation: School of Basic Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China.
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: Wu