title: Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics - An In-vitro Study.
abstract: BACKGROUND
  Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms.
  
  
  OBJECTIVE
  Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics.
  
  
  METHODS
  Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 - ZnOE, Group 2 - ZnOE +TAM, Group 3 - Apexit Plus, Group 4 - Apexit Plus +TAM, Group 5 - AH Plus, Group 6 - AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis.
  
  
  RESULTS
  ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect.
  
  
  CONCLUSIONS
  When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly.
authors.affiliation: Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Nalgonda, Telangana, India .
authors.forename: Amulya
authors.initials: A
authors.lastname: Vanapatla
authors.affiliation: Professor and HOD, Department of Conservative dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Nalgonda, Telangana, India .
authors.forename: Harikumar
authors.initials: H
authors.lastname: Vemisetty
authors.affiliation: Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Nalgonda, Telangana, India .
authors.forename: Rajani
authors.initials: R
authors.lastname: Punna
authors.affiliation: Professor and HOD, Department of Conservative Dentistry and Endodontics, Mamata Dental College , Khammam, Telangana, India .
authors.forename: Chandrasekhar
authors.initials: C
authors.lastname: Veeramachineni
authors.affiliation: Professor, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Nalgonda, Telangana, India .
authors.forename: Ravichandra Polavarapu
authors.initials: RP
authors.lastname: Venkata
authors.affiliation: Reader, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Nalgonda, Telangana, India .
authors.forename: Jaya Nagendra Krishna
authors.initials: JN
authors.lastname: Muppala
authors.affiliation: Reader, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Nalgonda, Telangana, India .
authors.forename: Ramkiran
authors.initials: R
authors.lastname: Dandolu
keywords: AH plus sealer; Apexit Plus sealer; Triple antibiotic mixture; Zinc oxide eugenol sealer