title: Selenium reduces enterohemorrhagic Escherichia coli O157:H7 verotoxin production and globotriaosylceramide receptor expression on host cells.
abstract: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined.
  
  
  
  The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR.
  
  
  
  Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Meera
authors.initials: M
authors.lastname: Surendran-Nair
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.|Department of Animal Science, University of Minnesota, St Paul, MN, USA.
authors.forename: Anup
authors.initials: A
authors.lastname: Kollanoor-Johny
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Ananda-Baskaran
authors.affiliation: Department of Molecular & Cell Biology, University of Connecticut, Storrs, CT, USA.
authors.forename: Carol
authors.initials: C
authors.lastname: Norris
authors.affiliation: Department of Nutritional Sciences, University of Connecticut, Storrs, CT, USA.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Kumar
authors.initials: K
authors.lastname: Venkitanarayanan
mesh_terms: D004927:Escherichia coli Infections / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012643:Selenium / Q000494:pharmacology*; D022603:Shiga Toxins / Q000378:metabolism*; D014281:Trihexosylceramides / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D022603:Shiga Toxins; D014281:Trihexosylceramides; C018549:globotriaosylceramide; D012643:Selenium
keywords: E. coli O157:H7; Gb3 receptor; selenium; verotoxin