title: A Novel Protein Elicitor (PeBA1) from Bacillus amyloliquefaciens NC6 Induces Systemic Resistance in Tobacco.
abstract: Here we reported a novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance (ISR) in tobacco. The purification was executed by ion-exchange chromatography, native-page extraction and HPLC, and the amino acid sequence was identified by mass spectrometry. This recombinant elicitor protein, expressed in Escherichia coli by an E1 expression vector, had good thermal stability, and the elicitor caused a clearly defined hypersensitive response (HR) necrosis in tobacco leaves. It could also trigger early defence events, including generation of reactive oxygen species (H2O2 and O2 (-)) and phenolic-compound accumulation. Quantitative real-time PCR (Q-RT-PCR) results indicated that several plant defence genes, including the salicylic acid (SA)-responsive PR1a, PR1b, PR5, and phenylalanine ammonia lyase (PAL), as well as the jasmonic acid (JA)-responsive PDF1.2 and CORONATINE INSENSITIVE 1 (COI1), were all up-regulated. Moreover, infiltration conferred systemic resistance against a broad spectrum of pathogens, including Tobacco mosaic virus (TMV) and the fungal pathogen Botrytis cinerea.
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Ningbo
authors.initials: N
authors.lastname: Wang
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Liu
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Guo
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Xiufen
authors.initials: X
authors.lastname: Yang
authors.affiliation: The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
mesh_terms: D000069977:Bacillus amyloliquefaciens / Q000276:immunology* / Q000378:metabolism* / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000276:immunology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007113:Immunity, Innate / Q000502:physiology; D054883:Oxylipins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014026:Nicotiana / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000276:immunology / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C011006:jasmonic acid; D006861:Hydrogen Peroxide
keywords: Hypersensitive response; ROS; induced systemic resistance; protein elicitor