title: A competitive luminol chemiluminescence immunosensor based on a microfluidic chip for the determination of ractopamine.
abstract: Herein, a competitive luminol chemiluminescence immunosensor based on a microfluidic chip was developed to detect ractopamine (RCT) both in phosphate buffer and swine urine samples. The immunosensor can provide a liner range of 0.5-40 ng/mL and a high sensitivity with a limit of detection of 0.97 ng/mL for RCT detection in swine urine. Good rates of recovery in negative swine urine samples were achieved over the RCT concentration ranging from 0.5 to 40 ng/mL. The proposed method offered a promising analytical scheme for the on-site determination of RCT.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
authors.forename: Sai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Laser Engineering, Beijing University of Technology, Beijing, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, P. R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, P. R. China.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000818:Animals; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000032:analysis / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010627:Phenethylamines / Q000652:urine*; D013552:Swine
chemical_list: D010627:Phenethylamines; C060897:ractopamine; D008165:Luminol
keywords: Immunosensor; Luminol chemiluminescence; Microfluidic chip; Ractopamine