title: Anticancer activity of Cynodon dactylon and Oxalis corniculata on Hep2 cell line.
abstract: Bioactive chemicals isolated from plants have attracted considerable attention over the years and overwhelmingly increasing laboratory findings are emphasizing on tumor suppressing properties of these natural agents in genetically and chemically induced animal carcinogenesis models. We studied in vitro anticancer activity of organic extracts of Cynodon dactylon and Oxalis corniculata on Hep2 cell line and it was compared with normal human corneal epithelial cells (HCEC) by using MTT assay. Real Time PCR was conducted for p53 and PTEN genes in treated cancer cell line. DNA fragmentation assay was also carried out to note DNA damaging effects of the extracts. The minimally effective concentration of ethanolic extract of Cynodon dactylon and methanolic extract of Oxalis corniculata that was nontoxic to HCEC but toxic to Hep2 was recorded (IC50) at a concentration of 0.042mg/ml (49.48 % cell death) and 0.048mg/ml (47.93% cell death) respectively, which was comparable to the positive control. Our results indicated dose dependent increase in cell death. P53 and PTEN did not show significant increase in treated cell line. Moreover, DNA damaging effects were also not detected in treated cancer cell line. Anticancer activity of these plants on the cancer cell line showed the presence of anticancer components which should be characterized to be used as anticancer therapy.
authors.affiliation: Institute of Biomedical and Genetic Engineering Islamabad Pakistan.
authors.forename: H
authors.initials: H
authors.lastname: Salahuddin
authors.affiliation: Institute of Biomedical and Genetic Engineering Islamabad Pakistan.
authors.forename: Q
authors.initials: Q
authors.lastname: Mansoor
authors.affiliation: Quaid-e-Azam University Department of Plant Sciences Islamabad Pakistan.
authors.forename: R
authors.initials: R
authors.lastname: Batool
authors.affiliation: Institute of Biomedical and Genetic Engineering Islamabad Pakistan.
authors.forename: A A
authors.initials: AA
authors.lastname: Farooqi
authors.affiliation: Quaid-e-Azam University Department of Plant Sciences Islamabad Pakistan.
authors.forename: T
authors.initials: T
authors.lastname: Mahmood
authors.affiliation: Institute of Biomedical and Genetic Engineering Islamabad Pakistan m.ismail02@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Ismail
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D031744:Cynodon / Q000737:chemistry*; D004249:DNA Damage; D006801:Humans; D065825:Oxalidaceae / Q000737:chemistry*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53; D051059:PTEN Phosphohydrolase; D020169:Caspases