title: Biological Characteristics of Experimental Genotype Mixtures of Cydia Pomonella Granulovirus (CpGV): Ability to Control Susceptible and Resistant Pest Populations.
abstract: The detection of resistance in codling moth (Cydia pomonella) populations against the Mexican isolate of its granulovirus (CpGV-M), raised questions on the sustainability of the use of this biological insecticide. In resistant host cells, CpGV-M is not able to complete its replication cycle because replication is blocked at an early step. Virus isolates able to overcome this resistance have been characterized-among them, the CpGV-R5 isolate. In mixed infections on resistant insects, both CpGV-M and CpGV-R5 viruses replicate, while CpGV-M alone does not induce mortality. Genetically heterogeneous virus populations, containing 50% of each CpGV-M and CpGV-R5 appear to control resistant host populations as well as CpGV-R5 alone at the same final concentration, even if the concentration of CpGV-R5 is only half in the former. The use of mixed genotype virus preparations instead of genotypically homogeneous populations may constitute a better approach than traditional methods for the development of baculovirus-based biological insecticides.
authors.affiliation: LGEI, Ecole des Mines d'Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6 Avenue de Clavières, 30319 Alès, France. graillot.benoit@gmail.com.|Natural Plant Protection, Arysta LifeScience Group, Avenue Léon Blum, 64000 Pau, France. graillot.benoit@gmail.com.
authors.forename: Benoit
authors.initials: B
authors.lastname: Graillot
authors.affiliation: LGEI, Ecole des Mines d'Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6 Avenue de Clavières, 30319 Alès, France. sandrine.bayle@mines-ales.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bayle
authors.affiliation: LGEI, Ecole des Mines d'Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6 Avenue de Clavières, 30319 Alès, France. christine.blachere-lopez@mines-ales.fr.|INRA, 6, Avenue de Clavières, 30319 Alès, France. christine.blachere-lopez@mines-ales.fr.
authors.forename: Christine
authors.initials: C
authors.lastname: Blachere-Lopez
authors.affiliation: Natural Plant Protection, Arysta LifeScience Group, Avenue Léon Blum, 64000 Pau, France. samantha.besse@arysta.com.
authors.forename: Samantha
authors.initials: S
authors.lastname: Besse
authors.affiliation: INRA, unité PSH, Agroparc, 84914 AVIGNON Cedex 9, France. myriam.siegwart@avignon.inra.fr.
authors.forename: Myriam
authors.initials: M
authors.lastname: Siegwart
authors.affiliation: LGEI, Ecole des Mines d'Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6 Avenue de Clavières, 30319 Alès, France. miguel.lopez-ferber@mines-ales.fr.
authors.forename: Miguel
authors.initials: M
authors.lastname: Lopez-Ferber
mesh_terms: D000818:Animals; D005838:Genotype; D025722:Granulovirus / Q000235:genetics* / Q000254:growth & development*; D007915:Lepidoptera / Q000821:virology*; D010572:Pest Control, Biological / Q000379:methods*; D016019:Survival Analysis; D019562:Viral Load
keywords: CpGV; Lepidoptera; biological control; genetic heterogeneity; resistance