title: Black cohosh inhibits 17β-estradiol-induced cell proliferation of endometrial adenocarcinoma cells.
abstract: This study was conducted to investigate the effect of black cohosh (BC) extract on the proliferation and apoptosis of Ishikawa cells. Ishikawa human endometrial adenocarcinoma cells were treated with or without BC (1, 5, 10 and 25 μM) and cell proliferation and cytotoxicity were measured by CCK-8 assays and flow cytometry analysis. Additionally, Ishikawa cells were treated with 17β-estradiol (E2), E2 + progesterone and E2 + BC (5 and 10 μM) and the effect of BC and progesterone on E2-induced cell proliferation was analyzed. BC decreased the proliferation of Ishikawa cells at a dose-dependent rate compared with the control group (p < 0.05). The proliferation of Ishikawa cells increased in the presence of E2, whereas the subsequent addition of progesterone or BC decreased proliferation to the level of the control group (p < 0.05). The inhibitory effect of BC on E2-induced cell proliferation was greater than the inhibitory effect of progesterone. In conclusion, BC induces apoptosis in Ishikawa cells and suppresses E2-induced cell proliferation in Ishikawa cells. BC could be considered a candidate co-treatment agent of estrogen-dependent tumors, especially those involving endometrial cells.
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: So Yun
authors.initials: SY
authors.lastname: Park
authors.affiliation: b Department of Physiology , Ewha Womans University, School of Medicine , Seoul , Republic of Korea.
authors.forename: Hee Ja
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Sa Ra
authors.initials: SR
authors.lastname: Lee
authors.affiliation: b Department of Physiology , Ewha Womans University, School of Medicine , Seoul , Republic of Korea.
authors.forename: Youn-Hee
authors.initials: YH
authors.lastname: Choi
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Kyungah
authors.initials: K
authors.lastname: Jeong
authors.affiliation: a Department of Obstetrics and Gynecology and.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Chung
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D031885:Cimicifuga; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D012739:Gonadal Steroid Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D012739:Gonadal Steroid Hormones; D010936:Plant Extracts; D011374:Progesterone; D004958:Estradiol
keywords: Endometrium; estrogen; menopause; uterus