title: Guar gum succinate-sodium alginate beads as a pH-sensitive carrier for colon-specific drug delivery.
abstract: Guar gum succinate - sodium alginate (GGS-SA) beads cross-linked with barium ions were prepared and characterized as a pH sensitive carrier for colon-specific drug delivery. The structure of GGS-SA beads was confirmed by FT-IR spectroscopy. Scanning Electron Microscope (SEM) studies revealed that the drug loaded GGS-SA beads prepared using 2:2 (w/v) weight percent of GGS and SA had a diameter about 1.4mm and roughly spherical in shape. X-ray diffraction (XRD) studies showed that the peaks corresponding to GGS and SA at 13.5°, 17.5°, 20.2° and 13.5°, 22°, 24.1°, respectively were destroyed in GGS-SA beads which show that these beads are more amorphous in nature. Swelling studies demonstrated the pH-dependent swelling behavior of GGS-SA beads. The beads showed higher swelling degrees in pH 7.4 than that in pH 1.2 due to the existence of anionic groups in the polymer chains. The drug release study showed that the amount of model drug, ibuprofen, released from the GGS-SA beads was higher in pH 7.4 than that in pH 1.2 due to the pH-dependent swelling behavior of the beads. MTT assay revealed that GGS-SA beads at a concentration range of 0-30μg/ml had no cytotoxic effect on the cultured mouse mesenchymal stem cells (C3H10T1/2). These results suggest that GGS-SA beads can be used as effective colon-specific drug delivery system with pH-dependent drug release ability.
authors.affiliation: Department of Chemistry, Hindustan Institute of Technology and Science, Padur, Chennai, 603 103, India.
authors.forename: D Sathya
authors.initials: DS
authors.lastname: Seeli
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203, India.
authors.forename: S
authors.initials: S
authors.lastname: Dhivya
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203, India.
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
authors.affiliation: Department of Chemistry, Hindustan Institute of Technology and Science, Padur, Chennai, 603 103, India. Electronic address: mprabaharan@yahoo.com.
authors.forename: M
authors.initials: M
authors.lastname: Prabaharan
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D002460:Cell Line; D003106:Colon / Q000378:metabolism*; D004008:Diclofenac / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D065546:Drug Liberation; D005685:Galactans / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000737:chemistry; D008351:Mannans / Q000737:chemistry*; D051379:Mice; D008863:Microspheres; D009928:Organ Specificity; D053149:Plant Gums / Q000737:chemistry*; D013386:Succinates / Q000737:chemistry*
chemical_list: D000464:Alginates; D004337:Drug Carriers; D005685:Galactans; D006603:Hexuronic Acids; D008351:Mannans; D053149:Plant Gums; D013386:Succinates; C000614811:guar gum succinate; D004008:Diclofenac; D020723:Glucuronic Acid; D007052:Ibuprofen
keywords: Colon specific; Cytotoxicity; Drug delivery; Guar gum succinate; Sodium alginate