title: Endothelial microparticles induce vascular endothelial cell injury in children with Kawasaki disease.
abstract: To explore the role of microparticles produced by endothelial cells in the injury of vascular endothelial cells.
  
  
  
  We stimulated human umbilical vein endothelial cells (HUVEC) with TNF-α in vitro, analyzed the change of cellular morphology, and measured EMP level in the supernatant with a flow cytometer. Then, we evaluated the corresponding clinical indicators and the role of EMP in endothelial injury.
  
  
  
  The endothelial cellular morphology underwent significant changes, and a large number of microparticles were secreted. In turn, these microparticles blocked cell cycle and induced apoptosis.
  
  
  
  The microparticles produced by endothelial cells play an important role in the injury of vascular endothelial cells.
authors.affiliation: Department of Cardiology, Xuzhou Children's Hospital, Xuzhou, Jiangsu, P.R. China. tal0979@163.com.
authors.forename: J
authors.initials: J
authors.lastname: Tian
authors.forename: H-T
authors.initials: HT
authors.lastname: Lv
authors.forename: X-J
authors.initials: XJ
authors.lastname: An
authors.forename: N
authors.initials: N
authors.lastname: Ling
authors.forename: F
authors.initials: F
authors.lastname: Xu
mesh_terms: D055252:Cell-Derived Microparticles; D002478:Cells, Cultured; D002648:Child; D042783:Endothelial Cells; D004730:Endothelium, Vascular / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D009080:Mucocutaneous Lymph Node Syndrome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha