title: Standard finishing categories for high-throughput sequencing of viral genomes.
abstract: Viral genome sequencing has become the cornerstone of almost all aspects of virology. In particular, high-throughput, next-generation viral genome sequencing has become an integral part of molecular epidemiological investigations into outbreaks of viral disease, such as the recent outbreaks of Middle Eastern respiratory syndrome, Ebola virus disease and Zika virus infection. Multiple institutes have acquired the expertise and necessary infrastructure to perform such investigations, as evidenced by the accumulation of thousands of novel viral sequences over progressively shorter time periods. The authors recently proposed a nomenclature comprised of five high-throughput sequencing standard categories to describe the quality of determined viral genome sequences. These five categories (standard draft, high quality, coding complete, complete and finished) cover all levels of viral genome finishing and can be applied to sequences determined by any technology platform or assembly technique.
authors.forename: J T
authors.initials: JT
authors.lastname: Ladner
authors.forename: J H
authors.initials: JH
authors.lastname: Kuhn
authors.forename: G
authors.initials: G
authors.lastname: Palacios
mesh_terms: D000818:Animals; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing / Q000145:classification / Q000379:methods / Q000662:veterinary*; D017720:Molecular Epidemiology; D009626:Terminology as Topic; D014777:Virus Diseases / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D014780:Viruses / Q000235:genetics*
keywords: Epidemiologie moleculaire; Genomique; Gestion de donnees; Sequencage; Sequencage haut-debit; Typage genomique; Virologie