title: Study on the interaction between three benzimidazole anthelmintics and eosin Y by high performance liquid chromatography associating with resonance light scattering and its application.
abstract: A novel, highly selective, and sensitive resonance light scattering (RLS) detection approach coupled with high performance liquid chromatography (HPLC) was researched and developed for the synchronous analysis of three kinds of benzimidazole anthelmintics, including mebendazole (MBZ), albendazole (ABZ), and fenbendazole (FBZ) for the first time. In the pH range of 3.5-3.7 Britton-Robinson buffer medium, three kinds of anthelmintics, which were separated by HPLC, reacted with eosin Y (EY) to form 1:1 ion-association complexes, resulting in significantly enhanced RLS signals and the maximum peak located at 335 nm. The enhanced RLS intensity was in proportion to the MBZ, ABZ, and FBZ concentration in the range 0.2-25, 0.2-23, and 0.15-20 μg/mL, respectively. The limit of detection was in the range of 0.064-0.16 μg/mL. In addition, human urine was determined to validate the proposed method by spiked samples and real urine samples. Satisfactory results were obtained by HPLC-RLS method. Graphical Abstract The diagram mechanism of generating resonance between emitted light and scattered light.
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, People's Republic of China.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Pan
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, People's Republic of China. hxpengjd@swu.edu.cn.
authors.forename: Jingdong
authors.initials: J
authors.lastname: Peng
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zang
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lei
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
mesh_terms: D000871:Anthelmintics / Q000032:analysis / Q000737:chemistry*; D001562:Benzimidazoles / Q000032:analysis / Q000737:chemistry*; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004801:Eosine Yellowish-(YS) / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D014556:Urine / Q000737:chemistry*
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D005456:Fluorescent Dyes; C031000:benzimidazole; D004801:Eosine Yellowish-(YS)
keywords: Benzimidazole anthelmintics; Eosin Y; High performance liquid chromatography; Resonance light scattering