title: Particulate systems based on pectin/chitosan association for the delivery of manuka honey components and platelet lysate in chronic skin ulcers.
abstract: The aim of the present work was the development of a powder formulation for the delivery of manuka honey (MH) bioactive components and platelet lysate (PL) in chronic skin ulcers. In particular pectin (PEC)/chitosan (CS) particles were prepared by ionotropic gelation in the presence of calcium chloride and subsequently characterized for particle size, hydration properties and mechanical resistance. Different experimental conditions (calcium chloride and CS concentrations; rest time in the cationic solution) were considered in order to obtain particles characterized by optimal size, hydration properties and mechanical resistance. Two different fractions of MH were examined: one (Fr1), rich in methylglyoxal and the other (Fr2), rich in polyphenols. Particles were loaded with Fr1, fraction able to enhance in vitro proliferation of human fibroblasts, and with PL. The presence of CS in Fr1-loaded particles produced an improvement in cell proliferation. Moreover, PL loading into particles did not affect the biological activity of the hemoderivative. In vivo efficacy of PL- and Fr1-loaded particles was evaluated on a rat wound model. Both treatments markedly increased wound healing to the same extent.
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Marika
authors.initials: M
authors.lastname: Tenci
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy. Electronic address: silvia.rossi@unipv.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bonferoni
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Boselli
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Di Lorenzo
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Daglia
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, Pavia, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Icaro Cornaglia
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, Pavia, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: Gioglio
authors.affiliation: Immunohaematology and Transfusion Service and Cell Therapy Unit of Fondazione IRCCS, S. Matteo, Pavia, Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Perotti
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects; D005782:Gels / Q000008:administration & dosage / Q000737:chemistry; D006722:Honey; D006801:Humans; D031649:Leptospermum / Q000737:chemistry*; D008297:Male; D010316:Particle Size; D010368:Pectins / Q000737:chemistry*; D028321:Plant Preparations / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012883:Skin Ulcer / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D005782:Gels; D028321:Plant Preparations; D010368:Pectins; D048271:Chitosan
keywords: Chitosan; Manuka honey; Pectin; Platelet lysate; Polymeric particles; Wound healing