title: Regulatory parameters of the lung immune response during the early phase of experimental trichinellosis.
abstract: Parasitic infection caused by Trichinella spiralis provokes an early stimulation of the mucosal immune system which causes an allergic inflammatory response in the lungs. The present work was intended to characterize the kinetics of emergence of regulatory parameters in Wistar rat lungs during this early inflammatory response, between days 0 and 13p.i. The presence of regulatory cells such as regulatory T cells (Tregs) and alternatively activated macrophages (AAM) was analyzed in lung cell suspensions. Moreover, a regulatory cytokine (TGF-β) was studied in lung tissue extracts. Considering that newborn larvae (NBL) travel along the pulmonary microvasculature, the ability of this parasite stage to modulate the activation of lung macrophages was evaluated. For this purpose, lung macrophages from non-infected or infected rats (day 6p.i.) were cultured with live or dead NBL. Arginase activity (characteristic of AAM) and nitric oxide (NO produced by iNOS, characteristic of classical activated macrophages) were measured after 48h. Our results revealed a significant increase in the percentage of Tregs on days 6 and 13p.i., arginase activity on day 13p.i. and TGF-β levels on days 6 and 13p.i. Lung macrophages from non-infected rats cultured with live NBL showed a significant increase in arginase activity and NO levels. Live and dead NBL induced a significant increase in arginase activity in lung macrophages from infected rats. Only live NBL significantly increased NO levels in these macrophages. The present work demonstrates for the first time, the emergence of regulatory parameters in the early lung immune response during T. spiralis infection. The immumodulatory properties exerted by NBL during its passage through this organ could be the cause of such regulation. Moreover, we have shown the ability of NBL to activate macrophages from the lung parenchyma by the classical and alternative pathways.
authors.affiliation: Microbiology, Immunology and Biotechnology Department, School of Pharmacy and Biochemistry, University of Buenos Aires, IDEHU-CONICET, Buenos Aires, Argentina.
authors.forename: Guido H
authors.initials: GH
authors.lastname: Falduto
authors.affiliation: Microbiology, Immunology and Biotechnology Department, School of Pharmacy and Biochemistry, University of Buenos Aires, IDEHU-CONICET, Buenos Aires, Argentina.
authors.forename: Cecilia C
authors.initials: CC
authors.lastname: Vila
authors.affiliation: Microbiology, Immunology and Biotechnology Department, School of Pharmacy and Biochemistry, University of Buenos Aires, IDEHU-CONICET, Buenos Aires, Argentina.
authors.forename: María P
authors.initials: MP
authors.lastname: Saracino
authors.affiliation: Microbiology, Immunology and Biotechnology Department, School of Pharmacy and Biochemistry, University of Buenos Aires, IDEHU-CONICET, Buenos Aires, Argentina.
authors.forename: María V
authors.initials: MV
authors.lastname: Gentilini
authors.affiliation: Microbiology, Immunology and Biotechnology Department, School of Pharmacy and Biochemistry, University of Buenos Aires, IDEHU-CONICET, Buenos Aires, Argentina. Electronic address: sventuri@ffyb.uba.ar.
authors.forename: Stella M
authors.initials: SM
authors.lastname: Venturiello
mesh_terms: D000818:Animals; D005260:Female; D007249:Inflammation / Q000276:immunology / Q000469:parasitology; D007814:Larva; D008168:Lung / Q000276:immunology*; D008174:Lung Diseases, Parasitic / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D008262:Macrophage Activation; D008264:Macrophages; D051381:Rats; D017208:Rats, Wistar; D014235:Trichinellosis / Q000276:immunology* / Q000473:pathology
keywords: Alternatively activated macrophages; Lung; Newborn larvae; Regulatory T cells; TGF-β; Trichinella spiralis