title: Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae).
abstract: OBJECTIVE
  We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador.
  
  
  RESULTS
  Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii.
  
  
  CONCLUSIONS
  We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.
authors.affiliation: Instituto Nacional de Investigaciones Agropecuarias (INIAP), Casilla Postal 17-01-340, Quito, Ecuador.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Morillo
authors.affiliation: Instituto Nacional de Investigaciones Agropecuarias (INIAP), Casilla Postal 17-01-340, Quito, Ecuador.
authors.forename: Johanna
authors.initials: J
authors.lastname: Buitron
authors.affiliation: Instituto Nacional de Investigaciones Agropecuarias (INIAP), Casilla Postal 17-01-340, Quito, Ecuador.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Limongi
authors.affiliation: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1334 AGAP, Montpellier, France.
authors.forename: Helene
authors.initials: H
authors.lastname: Vignes
authors.affiliation: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1334 AGAP, Montpellier, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Argout
keywords: Bignoniaceae; Handroanthus billbergii; microsatellites; next-generation sequencing (NGS)