title: Identification of Novel Changes in Human Skeletal Muscle Proteome After Roux-en-Y Gastric Bypass Surgery.
abstract: The mechanisms of metabolic improvements after Roux-en-Y gastric bypass (RYGB) surgery are not entirely clear. Therefore, the aim of our study was to investigate the role of obesity and RYGB on the human skeletal muscle proteome. Basal muscle biopsies were obtained from seven obese (BMI >40 kg/m(2)) female subjects (45.1 ± 3.6 years) pre- and 3 months post-RYGB, and euglycemic-hyperinsulinemic clamps were used to assess insulin sensitivity. Four age-matched (48.5 ± 4.7 years) lean (BMI <25 kg/m(2)) females served as control subjects. We performed quantitative mass spectrometry and microarray analyses on protein and RNA isolated from the muscle biopsies. Significant improvements in fasting plasma glucose (104.2 ± 7.8 vs. 86.7 ± 3.1 mg/dL) and BMI (42.1 ± 2.2 vs. 35.3 ± 1.8 kg/m(2)) were demonstrated in the pre- versus post-RYGB, both P < 0.05. Proteomic analysis identified 2,877 quantifiable proteins. Of these, 395 proteins were significantly altered in obesity before surgery, and 280 proteins differed significantly post-RYGB. Post-RYGB, 49 proteins were returned to normal levels after surgery. KEGG pathway analysis revealed a decreased abundance in ribosomal and oxidative phosphorylation proteins in obesity, and a normalization of ribosomal proteins post-RYGB. The transcriptomic data confirmed the normalization of the ribosomal proteins. Our results provide evidence that obesity and RYGB have a dynamic effect on the skeletal muscle proteome.
authors.affiliation: School of Life Sciences, Arizona State University, Tempe, AZ.
authors.forename: Latoya E
authors.initials: LE
authors.lastname: Campbell
authors.affiliation: Mayo Clinic, Scottsdale, AZ.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Langlais
authors.affiliation: School of Life Sciences, Arizona State University, Tempe, AZ.
authors.forename: Samantha E
authors.initials: SE
authors.lastname: Day
authors.affiliation: School for the Science of Health Care Delivery, Arizona State University, Phoenix, AZ.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Coletta
authors.affiliation: Mayo Clinic, Scottsdale, AZ.
authors.forename: Tonya R
authors.initials: TR
authors.lastname: Benjamin
authors.affiliation: Mayo Clinic, Scottsdale, AZ.
authors.forename: Elena Anna
authors.initials: EA
authors.lastname: De Filippis
authors.affiliation: Mayo Clinic Hospital, Phoenix, AZ.
authors.forename: James A
authors.initials: JA
authors.lastname: Madura
authors.affiliation: Mayo Clinic, Scottsdale, AZ School for the Science of Health Care Delivery, Arizona State University, Phoenix, AZ.
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Mandarino
authors.affiliation: Mayo Clinic, Scottsdale, AZ.
authors.forename: Lori R
authors.initials: LR
authors.lastname: Roust
authors.affiliation: Mayo Clinic, Scottsdale, AZ School for the Science of Health Care Delivery, Arizona State University, Phoenix, AZ Department of Basic Medical Sciences, University of Arizona College of Medicine-Phoenix, Phoenix, AZ dawn.coletta@asu.edu.
authors.forename: Dawn K
authors.initials: DK
authors.lastname: Coletta
mesh_terms: D001786:Blood Glucose / Q000378:metabolism; D005215:Fasting / Q000097:blood; D005260:Female; D015390:Gastric Bypass; D015309:Glucose Clamp Technique; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D046228:Microarray Analysis; D018482:Muscle, Skeletal / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D020543:Proteome