title: Mass Spectrometric Collisional Activation and Product Ion Mobility of Human Serum Neutral Lipid Extracts.
abstract: A novel method for lipid analysis called CTS (collisional activation and traveling wave mass spectrometry), involving tandem mass spectrometry of all precursor ions with ion mobility determinations of all product ions, was applied to a sample of human serum. The resulting four-dimensional data set (precursor ion, product ion, ion mobility value, and intensity) was found to be useful for characterization of lipids as classes as well as for identification of specific species. Utilization of ion mobility measurements of the product ions is a novel approach for lipid analysis. The trends and patterns of product mobility values when visually displayed yield information on lipid classes and specific species independent of mass determination. Collection of a comprehensive set of data that incorporates all precursor-product relationships, combined with ion mobility measurements of all products, enables data analysis where different molecular properties can be juxtaposed and analyzed to assist with class and species identification. Overall, CTS is a powerful, specific, and comprehensive method for lipid analysis.
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 East 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Hankin
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 East 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Barkley
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 East 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Karin
authors.initials: K
authors.lastname: Zemski-Berry
authors.affiliation: Department of Electrical Engineering, University of Colorado Denver , North Classroom Building, Room 2615, 1200 Larimer Street, Denver, Colorado 80202, United States.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 East 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Murphy
mesh_terms: D005231:Fatty Acids, Unsaturated / Q000097:blood / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry; D008055:Lipids / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014280:Triglycerides / Q000097:blood
chemical_list: D005231:Fatty Acids, Unsaturated; D007477:Ions; D008055:Lipids; D014280:Triglycerides