title: Regulation of MicroRNAs, and the Correlations of MicroRNAs and Their Targeted Genes by Zinc Oxide Nanoparticles in Ovarian Granulosa Cells.
abstract: Zinc oxide (ZnO) nanoparticles (NPs) have been applied in numerous industrial products and personal care products like sunscreens and cosmetics. The released ZnO NPs from consumer and household products into the environment might pose potential health issues for animals and humans. In this study the expression of microRNAs and the correlations of microRNAs and their targeted genes in ZnO NPs treated chicken ovarian granulosa cells were investigated. ZnSO4 was used as the sole Zn2+ provider to differentiate the effects of NPs from Zn2+. It was found that ZnO-NP-5 μg/ml specifically regulated the expression of microRNAs involved in embryonic development although ZnO-NP-5 μg/ml and ZnSO4-10 μg/ml treatments produced the same intracellular Zn concentrations and resulted in similar cell growth inhibition. And ZnO-NP-5 μg/ml also specifically regulated the correlations of microRNAs and their targeted genes. This is the first investigation that intact NPs in ZnO-NP-5 μg/ml treatment specifically regulated the expression of microRNAs, and the correlations of microRNAs and their targeted genes compared to that by Zn2+. This expands our knowledge for biological effects of ZnO NPs and at the same time it raises the health concerns that ZnO NPs might adversely affect our biological systems, even the reproductive systems through regulation of specific signaling pathways.
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, P. R. China.|Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.
authors.forename: Ling-Jiang
authors.initials: LJ
authors.lastname: Min
authors.affiliation: College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Lian-Qin
authors.initials: LQ
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.|State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P. R. China.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Hong-Fu
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, P. R. China.|Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao, 266109, P. R. China.
authors.forename: Jing-Cai
authors.initials: JC
authors.lastname: Liu
authors.affiliation: College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
authors.forename: Zhi-Hui
authors.identifier: 0000-0001-7131-5219
authors.initials: ZH
authors.lastname: Hao
mesh_terms: D000818:Animals; D002470:Cell Survival; D002645:Chickens; D005260:Female; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009711:Nucleotides / Q000235:genetics; D010053:Ovary / Q000166:cytology* / Q000187:drug effects*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D015032:Zinc / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D009711:Nucleotides; D015032:Zinc; D015034:Zinc Oxide