title: Inverse Expression Levels of EphrinA3 and EphrinA5 Contribute to Dopaminergic Differentiation of Human SH-SY5Y Cells.
abstract: Two key principles underlying successful cellular therapies for Parkinson's disease (PD) are appropriate differentiation of dopaminergic (DA) neurons from transplanted cells and precise axon growth. EphrinAs, a subclass of ephrins, act as axon guidance molecules and are highly expressed in DA brain regions. Existing evidences indicate that they act as either repulsion or attraction signals to guide axon growth. This study investigated whether ephrinAs are involved in DA neuron differentiation. Data from miRCURY™ LNA mRNAs/microRNAs microarrays and quantitative real-time polymerase chain reaction (qRT-PCR) showed upregulated ephrinA3 mRNA (EFNA3) and downregulated ephrinA5 mRNA (EFNA5) during DA neuron differentiation. In addition, hsa-miR-4271 was downregulated, which could influence EFNA3 translation. Furthermore, immunofluorescence (IF) and western blotting confirmed the mRNA results and showed increased ephrinA3 and decreased ephrinA5 protein levels in differentiating DA neurons. Taken together, our results indicate that inverse expression levels of ephrinA3 and ephrinA5, which are possibly influenced by microRNAs, contribute to DA neuron differentiation by guiding axon growth.
authors.affiliation: Department of Human Anatomy and Neurobiology, Xuzhou Medical College, Tongshan Road 209#, Xuzhou, 221009, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Experimental Teaching Center of Morphology, Xuzhou Medical College, Tongshan Road 209#, Xuzhou, 221009, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Human Anatomy and Neurobiology, Xuzhou Medical College, Tongshan Road 209#, Xuzhou, 221009, China.
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Tang
authors.affiliation: Experimental Teaching Center of Morphology, Xuzhou Medical College, Tongshan Road 209#, Xuzhou, 221009, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhou
authors.affiliation: Department of Human Anatomy and Neurobiology, Xuzhou Medical College, Tongshan Road 209#, Xuzhou, 221009, China. gds@xzmc.edu.cn.
authors.forename: Dian-Shuai
authors.initials: DS
authors.lastname: Gao
mesh_terms: D002454:Cell Differentiation; D045744:Cell Line, Tumor; D059290:Dopaminergic Neurons / Q000166:cytology / Q000378:metabolism*; D036384:Ephrin-A3 / Q000235:genetics / Q000378:metabolism*; D036386:Ephrin-A5 / Q000235:genetics / Q000378:metabolism*; D006801:Humans
chemical_list: D036384:Ephrin-A3; D036386:Ephrin-A5
keywords: Axon guidance; Cellular therapy; EphrinAs; Parkinson’s disease