title: Laboratory course on Streptomyces genetics and secondary metabolism.
abstract: The "Streptomyces genetics and secondary metabolism" laboratory course gives an introduction to the versatile soil dwelling Gram-positive bacteria Streptomyces and their secondary metabolism. The course combines genetic modification of Streptomyces; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains. Thus, the course is a combination of microbiology, molecular biology, and chemistry. After the course the students should understand the relationship between genes, proteins, and the produced metabolites. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(5):492-499, 2016.
authors.affiliation: Department of Biochemistry, University of Turku, Turku, FIN-20014, Finland. vilja.siitonen@gmail.com.
authors.forename: Vilja
authors.initials: V
authors.lastname: Siitonen
authors.affiliation: Department of Biochemistry, University of Turku, Turku, FIN-20014, Finland.
authors.forename: Kaj
authors.initials: K
authors.lastname: Räty
authors.affiliation: Department of Biochemistry, University of Turku, Turku, FIN-20014, Finland.
authors.forename: Mikko
authors.initials: M
authors.lastname: Metsä-Ketelä
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D035843:Biomedical Research / Q000193:education*; D002621:Chemistry / Q000193:education*; D002855:Chromatography, Thin Layer; D003479:Curriculum; D004521:Educational Measurement; D005823:Genetics / Q000193:education*; D006801:Humans; D007753:Laboratories; D008829:Microbiology / Q000193:education*; D008954:Models, Biological; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D018794:Problem-Based Learning / Q000379:methods; D064210:Secondary Metabolism; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013334:Students / Q000523:psychology; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins
keywords: Streptomyces; nogalamycin; rhodomycin; secondary metabolites