title: miR-30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse.
abstract: OBJECTIVE
  Mechanisms that regulate proliferation of adult neural stem cells are largely unknown. Here, we have investigated the role of microR-30c (miR-30c) and its target, semaphoring 3A (sema3A), in regulating adult neurogenesis and mechanisms underlying this process.
  
  
  METHODS
  In situ hybridization, immunofluorescence and quantitative real-time PCR were used to assess complementary expression patterns of miR-30c and sema3A in mice. Effects of miR-30c in the subventricular zone (SVZ) were examined by stereotaxic injection of up- and down-regulating lentiviruses. 5'-bromo-2'-deoxyuridine labelling was performed to investigate effects of miR-30c and sema3A on adult neurogenesis. Real-time cell assays, morphological analysis and cell cycle measurements were used to reveal the mechanisms by which miR-30c and sema3A regulate adult neurogenesis.
  
  
  RESULTS
  Expression of miR-30c negatively correlated with that of sema3A in neurons, and levels of miR-30c and sema3A correlated positively with numbers of newborn cells in the SVZ and rostral migration stream. miR-30c and sema3A affected adult neurogenesis by regulating proliferation and differentiation, as well as cycles of stem cells in the SVZ.
  
  
  CONCLUSIONS
  miR-30c and sema3A regulate adult neurogenesis by controlling proliferation and differentiation of stem cells in the SVZ. This finding reveals a novel regulatory mechanism of adult neurogenesis.
authors.affiliation: Institute of Neuroscience and Anatomy, Zhejiang University, School of Medicine, Hangzhou, 310058, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sun
authors.affiliation: Institute of Neuroscience and Anatomy, Zhejiang University, School of Medicine, Hangzhou, 310058, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Neuroscience and Anatomy, Zhejiang University, School of Medicine, Hangzhou, 310058, China.
authors.forename: Shucai
authors.initials: S
authors.lastname: Ling
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020547:Lateral Ventricles / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000032:analysis / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis; D040121:Semaphorin-3A / Q000032:analysis / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; C534715:Mirn30d microRNA, mouse; D040121:Semaphorin-3A