title: MARIS plays important roles in Arabidopsis pollen tube and root hair growth.
abstract: In flowering plants, male gametes are delivered to female gametes for double fertilization through pollen tubes. Therefore, pollen tube growth is crucial for double fertilization. Despite its importance to sexual reproduction, genetic mechanisms of pollen tube growth remain poorly understood. In this study, we characterized the receptor-like cytoplasmic protein kinase (RLCK) gene, MARIS (MRI) that plays critical roles in pollen tube growth. MRI is preferentially expressed in pollen grains, pollen tubes and roots. Mutation in MRI by a Ds insertion led to a burst of pollen tubes after pollen germination. Pollen-rescue assay by pollen and pollen tube-specific expression of MRI in the mri-4 mutant showed that loss of MRI function also severely affected root hair elongation. MRI protein interacted with the protein kinase OXIDATIVE SIGNAL INDUCIBLE1 (OXI1) in the in vitro and in vivo assays, which functions in plant defence and root hair development, and was phosphorylated by OXI1 in vitro. Our results suggest that MRI plays important roles in pollen tube growth and may function in root hair elongation through interaction with OXI1.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Hong-Ze
authors.initials: HZ
authors.lastname: Liao
authors.affiliation: Department of Biological Science and Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Du
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: De
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. kouyang@cau.edu.cn.
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Zhang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D053205:Pollen Tube / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000604170:MARIS protein, Arabidopsis; D011494:Protein Kinases
keywords: Arabidopsis; pollen tube; protein kinase; receptor-like cytoplasmic protein kinases; root hair