title: Profiling circulating miRNAs in serum from pigs infected with the porcine whipworm, Trichuris suis.
abstract: microRNAs (miRNAs) are recently discovered as key regulators of gene translation and are becoming increasingly recognized for their involvement in various diseases. This study investigates the miRNA profile in pig serum during the course of an infection with the gastrointestinal parasite, Trichuris suis. Of this panel, the expression of selected miRNAs in serum from T. suis infected and uninfected pigs were determined by quantitative real time PCR using Exiqon Human Panel assays at 0, 2, 4, 6, 8 and 10 weeks post first infection (wpi). One miRNA, ssc-let-7d-3p, was significantly up-regulated in infected pigs 8 wpi. Interestingly, ssc-let-7d-3p shows high complementary to tsu-let-7a, which is the most highly transcribed miRNA in T. suis. The let-7 family miRNAs have been shown to post-transcriptionally regulate the translation of the helminth-controlling cytokine, IL-13, in a murine model for asthma and we hypothesize possible interactions between these host- and parasite-derived miRNAs and their immunomodulating roles.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Copenhagen, Denmark. Electronic address: eph@sund.ku.dk.
authors.forename: Eline Palm
authors.initials: EP
authors.lastname: Hansen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Copenhagen, Denmark.
authors.forename: Helene
authors.initials: H
authors.lastname: Kringel
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Copenhagen, Denmark.
authors.forename: Stig Milan
authors.initials: SM
authors.lastname: Thamsborg
authors.affiliation: Population Health and Immunity Division, The Walter and Eliza Hall Institute, Parkville, Victoria 3010, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Aaron
authors.initials: A
authors.lastname: Jex
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Copenhagen, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Nejsum
mesh_terms: D000818:Animals; D015415:Biomarkers; D005260:Female; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000469:parasitology*; D014257:Trichuriasis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D014258:Trichuris
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Biomarker; Pig; Trichuris suis; Whipworm; miRNA