title: Performance Evaluation of the FREND Cardiac Triple Cartridge on the FREND System.
abstract: BACKGROUND
  We evaluated the performance of the FREND Cardiac Triple cartridge on the FREND system in the detection of cardiac markers-myoglobin, cardiac troponin I (cTnI), and creatine kinase-MB (CK-MB).
  
  
  METHODS
  Quantitative immunoassays were performed using the FREND system (NanoEnTek, Seoul, Korea) and its cartridge. The precision, detection limits, linearity, and correlation with the Siemens Dimension Vista 500 (Siemens Healthcare Diagnostics, Deerfield, IL, USA) were evaluated. The cutoff value for each marker was calculated in healthy individuals (men and women, n = 138 each).
  
  
  RESULTS
  The coefficients of variation for imprecision were less than 19.0% at low and high serum concentrations. The lower limits of quantification for myoglobin, cTnI, and CK-MB were 3.11, 0.073, and 0.70 ng/mL, respectively. Acceptable linearity was achieved for each marker (R2 < 0.99). The results from the FREND system were in good agreement with those from the Siemens Dimension Vista (correlation coefficients > 0.9). The cutoff values in male and female individuals (n = 138 each) were 104.3 and 98.9 ng/mL, respectively, for myoglobin, and 4.35 and 5.37 ng/mL, respectively, for CK-MB. The cutoff value for cTnI was 0.073 ng/mL.
  
  
  CONCLUSIONS
  The FREND Cardiac Triple cartridge exhibited good precision, clinically acceptable linearity, and reliable correlation with the Dimension Vista.
authors.forename: Kyunghoon
authors.initials: K
authors.lastname: Lee
authors.forename: Minje
authors.initials: M
authors.lastname: Han
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Song
authors.forename: Kyoung Un
authors.initials: KU
authors.lastname: Park
authors.forename: Woon Heung
authors.initials: WH
authors.lastname: Song
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
mesh_terms: D052279:Creatine Kinase, MB Form / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D009211:Myoglobin / Q000097:blood*; D019095:Point-of-Care Systems; D019210:Troponin I / Q000097:blood*
chemical_list: D009211:Myoglobin; D019210:Troponin I; D052279:Creatine Kinase, MB Form