title: Efficient synthesis of longer Aβ peptides via removable backbone modification.
abstract: Longer amyloid-beta (Aβ) peptides (43 to 49 amino acids) play essential roles in the pathology of Alzheimer's disease (AD). The difficulty in the preparation of longer Aβ peptides is still an obstacle to elucidate their roles in AD. Herein we report a robust and efficient strategy for the chemical synthesis of longer Aβ peptides (Aβ48 and Aβ49). A key feature of this method is the installation of removable Arg4-tagged backbone modification groups into the hydrophobic region of Aβ. This modification can improve the handling properties of the purification, ligation and mass characterization of longer Aβ peptides. The practicability of the new method has been demonstrated by the successful synthesis of Aβ48 and Aβ49 peptides.
authors.affiliation: High Magnetic Field Laboratory, Chinese Academy of Sciences, School of Life Sciences, University of Science and Technology of China, Hefei 230026, China. jszheng@hmfl.cas.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Zuo
authors.forename: Shan
authors.initials: S
authors.lastname: Tang
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Si
authors.forename: Zhipeng A
authors.initials: ZA
authors.lastname: Wang
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Tian
authors.forename: Ji-Shen
authors.initials: JS
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic; D057927:Hydrophobic and Hydrophilic Interactions
chemical_list: D016229:Amyloid beta-Peptides; D001120:Arginine