title: Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells.
abstract: OBJECTIVE
  To explore the value of transplanting peripheral blood-derived mesenchymal stem cells from allogenic rabbits (rPBMSCs) to treat osteonecrosis of the femoral head (ONFH).
  
  
  METHODS
  rPBMSCs were separated/cultured from peripheral blood after granulocyte colony-stimulating factor mobilization. Afterwards, mobilized rPBMSCs from a second passage labeled with PKH26 were transplanted into rabbit ONFH models, which were established by liquid nitrogen freezing, to observe the effect of rPBMSCs on ONFH repair. Then, the mRNA expressions of BMP-2 and PPAR-γ in the femoral head were assessed by RT-PCR.
  
  
  RESULTS
  After mobilization, the cultured rPBMSCs expressed mesenchymal markers of CD90, CD44, CD29, and CD105, but failed to express CD45, CD14, and CD34. The colony forming efficiency of mobilized rPBMSCs ranged from 2.8 to 10.8 per million peripheral mononuclear cells. After local transplantation, survival of the engrafted cells reached at least 8 weeks. Therein, BMP-2 was up-regulated, while PPAR-γ mRNA was down-regulated. Additionally, bone density and bone trabeculae tended to increase gradually.
  
  
  CONCLUSIONS
  We confirmed that local transplantation of rPBMSCs benefits ONFH treatment and that the beneficial effects are related to the up-regulation of BMP-2 expression and the down-regulation of PPAR-γ expression.
authors.affiliation: Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Ning Ning
authors.initials: NN
authors.lastname: Tang
authors.affiliation: Department of Human Anatomy, Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Jia Chen
authors.initials: JC
authors.lastname: Peng
authors.affiliation: Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Fang
authors.affiliation: Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Li Mei
authors.initials: LM
authors.lastname: Yu
authors.affiliation: Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China.
authors.forename: Jin Wei
authors.initials: JW
authors.lastname: Liu
authors.affiliation: Key Laboratory of Cell Engineering of Guizhou Province, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou, China. oceanzt@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics; D064987:Cell- and Tissue-Based Therapy; D005271:Femur Head Necrosis / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D005786:Gene Expression Regulation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010020:Osteonecrosis / Q000473:pathology* / Q000628:therapy*; D047495:PPAR gamma / Q000235:genetics; D011817:Rabbits; D014184:Transplantation, Homologous
chemical_list: D055396:Bone Morphogenetic Protein 2; D047495:PPAR gamma
keywords: Osteonecrosis of the femoral head (ONFH); cell transplantation; disease model; mesenchymal stem cells (MSCs); peripheral blood; rabbit