title: Establishing Quantitative Standards for Residual Alkaline Phosphatase in Pasteurized Milk.
abstract: The alkaline phosphatase (ALP) assay is a rapid and convenient method for verifying milk pasteurization. Since colorimetric ALP assays rely on subjective visual assessments, their results are especially unreliable near the detection limits. In this study, we attempted to establish quantitative criteria for residual ALP in milk by using a more objective method based on spectrophotometric measurements. Raw milk was heat-treated for 0, 10, 20, 30, and 40 min and then subjected to ALP assays. The quantitative criteria for residual ALP in the milk was determined as 2 μg phenol/mL of milk, which is just above the ALP value of milk samples heat-treated for 30 min. These newly proposed methodology and criteria could facilitate the microbiological quality control of milk.
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Technology, Konkuk University, Seoul 05029, Korea.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Il-Byeong
authors.initials: IB
authors.lastname: Kang
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Dana
authors.initials: D
authors.lastname: Jeong
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Kwang-Young
authors.initials: KY
authors.lastname: Song
authors.affiliation: Department of Food & Nutrition, College of Human Ecology, Hanyang University, Seoul 04763, Korea.
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Kwang-Yup
authors.initials: KY
authors.lastname: Kim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
keywords: alkaline phosphatase; milk; pasteurization; quantitative criteria; spectrophotometer