title: Ginsenoside Rg1 and platelet-rich fibrin enhance human breast adipose-derived stem cell function for soft tissue regeneration.
abstract: Adipose-derived stem cells (ASCs) can be used to repair soft tissue defects, wounds, burns, and scars and to regenerate various damaged tissues. The cell differentiation capacity of ASCs is crucial for engineered adipose tissue regeneration in reconstructive and plastic surgery. We previously reported that ginsenoside Rg1 (G-Rg1 or Rg1) promotes proliferation and differentiation of ASCs in vitro and in vivio. Here we show that both G-Rg1 and platelet-rich fibrin (PRF) improve the proliferation, differentiation, and soft tissue regeneration capacity of human breast adipose-derived stem cells (HBASCs) on collagen type I sponge scaffolds in vitro and in vivo. Three months after transplantation, tissue wet weight, adipocyte number, intracellular lipid, microvessel density, and gene and protein expression of VEGF, HIF-1α, and PPARγ were higher in both G-Rg1- and PRF-treated HBASCs than in control grafts. More extensive new adipose tissue formation was evident after treatment with G-Rg1 or PRF. In summary, G-Rg1 and/or PRF co-administration improves the function of HBASCs for soft tissue regeneration engineering.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China.
authors.forename: Fang-Tian
authors.initials: FT
authors.lastname: Xu
authors.affiliation: Department of Hepatobiliary and Gland Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People's Hospital of Nanning, Nanning 530022, China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Liang
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People's Hospital of Nanning, Nanning 530022, China.
authors.forename: Hong-Mian
authors.initials: HM
authors.lastname: Li
authors.affiliation: Central Laboratory of Medical Science, The Fifth Affiliated Hospital of Guangxi Medical University and The First People's Hospital of Nanning, Nanning 530022, China.
authors.forename: Qi-Liu
authors.initials: QL
authors.lastname: Peng
authors.affiliation: Department of Hepatobiliary and Gland Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People's Hospital of Nanning, Nanning 530022, China.
authors.forename: Min-Hong
authors.initials: MH
authors.lastname: Huang
authors.affiliation: Department of Breast Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: De Quan
authors.initials: de Q
authors.lastname: Li
authors.affiliation: Central Laboratory of Medical Science, The Fifth Affiliated Hospital of Guangxi Medical University and The First People's Hospital of Nanning, Nanning 530022, China.
authors.forename: Yi-Dan
authors.initials: YD
authors.lastname: Liang
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University and The First People's Hospital of Nanning, Nanning 530022, China.
authors.forename: Gang-Yi
authors.initials: GY
authors.lastname: Chi
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: De Hui
authors.initials: de H
authors.lastname: Li
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Bing-Chao
authors.initials: BC
authors.lastname: Yu
authors.affiliation: Department of Burns and Plastic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Ji-Rong
authors.initials: JR
authors.lastname: Huang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001940:Breast; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D064593:Heterografts; D006801:Humans; D008819:Mice, Nude; D000073183:Platelet-Rich Fibrin; D012038:Regeneration / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D036145:Ginsenosides; C035054:ginsenoside Rg1
keywords: collagen type I sponge scaffolds; ginsenoside Rg1; human breast adipose-derived stem cells; platelet rich fibrin; soft tissue regeneration