title: BRAF Mutation (V600E) Prevalence in Mexican Patients Diagnosed with Melanoma.
abstract: BACKGROUND
  B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation.
  
  
  OBJECTIVE
  The goal of this research was to determine the prevalence of the BRAF V600E mutation in patients from Central Mexico diagnosed with primary melanoma.
  
  
  METHODS
  Skin biopsies from 47 patients with melanoma were obtained from the dermatology department of the Hospital General 'Dr. Manuel Gea González' in Mexico City. For BRAF mutation determination, after DNA isolation, the gene region where the mutation occurs was amplified by PCR. Subsequently, the presence or absence of the V600E mutation was detected by Sanger sequencing performed at the private molecular diagnostic laboratory Vitagénesis in Monterrey, Mexico.
  
  
  RESULTS
  Of the 47 patients sampled, 6.4% harbored the V600E mutation. No statistical significance was found between mutations and the type of tumor.
authors.affiliation: Servicio de Dermatología, Hospital General 'Dr. Manuel Gea González', S.A. de C.V., Mexico City, Monterrey, Mexico.
authors.forename: Priscilla Denise
authors.initials: PD
authors.lastname: Zepeda-Lopez
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, San Pedro Garza García, Monterrey, Mexico.
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: Salas-Alanis
authors.affiliation: Servicio de Dermatología, Hospital General 'Dr. Manuel Gea González', S.A. de C.V., Mexico City, Monterrey, Mexico.
authors.forename: Sonia
authors.initials: S
authors.lastname: Toussaint-Caire
authors.affiliation: Servicio de Dermatología, Hospital General 'Dr. Manuel Gea González', S.A. de C.V., Mexico City, Monterrey, Mexico.
authors.forename: Daniela
authors.initials: D
authors.lastname: Gutierrez-Mendoza
authors.affiliation: Servicio de Dermatología, Hospital General 'Dr. Manuel Gea González', S.A. de C.V., Mexico City, Monterrey, Mexico.
authors.forename: Elisa
authors.initials: E
authors.lastname: Vega-Memije
authors.affiliation: Departamento de Patología, Instituto Nacional de Cancerología, Mexico City, Monterrey, Mexico.
authors.forename: Saúl Lino
authors.initials: SL
authors.lastname: Silva
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, San Pedro Garza García, Monterrey, Mexico.
authors.forename: Oscar Raul
authors.initials: OR
authors.lastname: Fajardo-Ramírez
authors.affiliation: Vitagénesis, S.A. de C.V., Monterrey, Mexico.
authors.forename: Gregorio
authors.initials: G
authors.lastname: Alcazar
authors.affiliation: Departamento de Ciencias Básicas, Universidad de Monterrey, San Pedro Garza García, Monterrey, Mexico.
authors.forename: María Guadalupe
authors.initials: MG
authors.lastname: Moreno-Treviño
authors.affiliation: Vitagénesis, S.A. de C.V., Monterrey, Mexico.
authors.forename: Hugo Alberto Barrera
authors.initials: HA
authors.lastname: Saldaña
keywords: BRAF; Melanoma; Mexican patients; V600E mutation