title: Synthesis of tumor necrosis factor α for use as a mirror-image phage display target.
abstract: Tumor Necrosis Factor alpha (TNFα) is an inflammatory cytokine that plays a central role in the pathogenesis of chronic inflammatory disease. Here we describe the chemical synthesis of l-TNFα along with the mirror-image d-protein for use as a phage display target. The synthetic strategy utilized native chemical ligation and desulfurization to unite three peptide segments, followed by oxidative folding to assemble the 52 kDa homotrimeric protein. This synthesis represents the foundational step for discovering an inhibitory d-peptide with the potential to improve current anti-TNFα therapeutic strategies.
authors.affiliation: Department of Biochemistry, University of Utah, 15 N Medical Drive East, Rm 4100, Salt Lake City, Utah 84112-5650, USA. Kay@biochem.utah.edu.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Petersen
authors.forename: Michael T
authors.initials: MT
authors.lastname: Jacobsen
authors.forename: Michael S
authors.initials: MS
authors.lastname: Kay
mesh_terms: D008958:Models, Molecular; D019151:Peptide Library; D017433:Protein Structure, Secondary; D013237:Stereoisomerism; D014409:Tumor Necrosis Factor-alpha / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D019151:Peptide Library; D014409:Tumor Necrosis Factor-alpha