title: Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801.
abstract: In high-quality sake brewing, the cerulenin-resistant sake yeast K1801 with high ethyl caproate-producing ability has been used widely; however, K1801 has a defective spindle assembly checkpoint (SAC). To identify the mutation causing this defect, we first searched for sake yeasts with a SAC-defect like K1801 and found that K13 had such a defect. Then, we searched for a common SNP in only K1801 and K13 by examining 15 checkpoint-related genes in 23 sake yeasts, and found 1 mutation, R48P of Cdc55, the PP2A regulatory B subunit that is important for the SAC. Furthermore, we confirmed that the Cdc55-R48P mutation was responsible for the SAC-defect in K1801 by molecular genetic analyses. Morphological analysis indicated that this mutation caused a high cell morphological variation. But this mutation did not affect the excellent brewing properties of K1801. Thus, this mutation is a target for breeding of a new risk-free K1801 with normal checkpoint integrity.
authors.affiliation: a National Research Institute of Brewing , Higashi-Hiroshima , Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Goshima
authors.affiliation: b Research and Development Department , Asahi Sake Brewing Co. Ltd. , Nagaoka , Niigata , Japan.|c Department of Molecular Biotechnology , Graduate School of Advanced Sciences of Matter, Hiroshima University , Higashi-Hiroshima , Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakamura
authors.affiliation: c Department of Molecular Biotechnology , Graduate School of Advanced Sciences of Matter, Hiroshima University , Higashi-Hiroshima , Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kume
authors.affiliation: d Department of Integrated Biosciences, Graduate School of Frontier Sciences , The University of Tokyo , Kashiwa , Chiba , Japan.|e Department of Cell and Developmental Biology, Perelman School of Medicine , University of Pennsylvania , Philadelphia , PA , USA.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okada
authors.affiliation: b Research and Development Department , Asahi Sake Brewing Co. Ltd. , Nagaoka , Niigata , Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Ichikawa
authors.affiliation: b Research and Development Department , Asahi Sake Brewing Co. Ltd. , Nagaoka , Niigata , Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Tamura
authors.affiliation: f Brewing Society of Japan , Kita-ku , Tokyo , Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hasuda
authors.affiliation: f Brewing Society of Japan , Kita-ku , Tokyo , Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Inahashi
authors.affiliation: f Brewing Society of Japan , Kita-ku , Tokyo , Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Okazaki
authors.affiliation: a National Research Institute of Brewing , Higashi-Hiroshima , Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Akao
authors.affiliation: g Department of Biological Chemistry and Food Sciences , Iwate University , Morioka , Iwate , Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shimoi
authors.affiliation: c Department of Molecular Biotechnology , Graduate School of Advanced Sciences of Matter, Hiroshima University , Higashi-Hiroshima , Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Mizunuma
authors.affiliation: d Department of Integrated Biosciences, Graduate School of Frontier Sciences , The University of Tokyo , Kashiwa , Chiba , Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
authors.affiliation: b Research and Development Department , Asahi Sake Brewing Co. Ltd. , Nagaoka , Niigata , Japan.|c Department of Molecular Biotechnology , Graduate School of Advanced Sciences of Matter, Hiroshima University , Higashi-Hiroshima , Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Hirata
mesh_terms: D000434:Alcoholic Beverages; D000595:Amino Acid Sequence; D001483:Base Sequence; D002208:Caproates / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005524:Food Technology; D015870:Gene Expression; D006801:Humans; D007564:Japan; D059566:M Phase Cell Cycle Checkpoints; D009154:Mutation; D009812:Odorants; D012275:Oryza / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D054648:Protein Phosphatase 2 / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D012641:Selection, Genetic
chemical_list: C071246:CDC55 protein, S cerevisiae; D002208:Caproates; D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D054648:Protein Phosphatase 2; C079237:ethyl hexanoate
keywords: cerulenin-resistant; checkpoint; ethyl caproate; morphological variation; sake yeast