title: Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli.
abstract: Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase-acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20±0.02mol/mol) than glucose (0.48±0.03mol/mol). The concentration and molar yield of succinic acid were 22.40±0.12g/L and 1.13±0.02mol/mol total sugar respectively after 72h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57±0.17g/L) and galactose (18.03±0.10g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Ayobami Matthew
authors.initials: AM
authors.lastname: Olajuyin
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Maohua
authors.initials: M
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Yilan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Tingzhen
authors.initials: T
authors.lastname: Mu
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria.
authors.forename: Oluwatosin Adekunle
authors.initials: OA
authors.lastname: Adaramoye
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: jmxing@ipe.ac.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D060847:Metabolic Engineering / Q000379:methods; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism; D000461:Rhodophyta / Q000737:chemistry* / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D004798:Enzymes; D029968:Escherichia coli Proteins; D019802:Succinic Acid; D000123:Acetyltransferases; C022560:formate C-acetyltransferase; D010730:Phosphoenolpyruvate Carboxylase; D005947:Glucose; D005690:Galactose
keywords: Escherichia coli; Fermentation; Galactose; Palmaria palmata; Succinic acid