title: A rifampicin-resistant (rpoB) mutation in Pseudomonas protegens Pf-5 strain leads to improved antifungal activity and elevated production of secondary metabolites.
abstract: Ribosome engineering has proven to be a practical method for increasing antibiotic production, and is extensively applied to strain improvement in antibiotic production and activation of silent genes in several prokaryotes. In this study, ribosome engineering was used to improve production of bioactive secondary metabolites produced by Pseudomonas protegens Pf-5. Rifampicin-resistant mutants that bear the H531N in the β-subunit of RNA polymerase showed improved antifungal activity and morphological changes. The production of several secondary metabolites in R55 mutant was significantly improved using high-performance liquid chromatography (HPLC) analysis. Two antibiotics with antifungal activity, 2, 4-diacetylphloroglucinol (Phl) and pyoluteorin (Plt), which may contribute to the improved antifungal activity of the R55 mutant, were identified by mass spectrometer (MS) analysis.
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Xie
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Guoyong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Mo
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Ding
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China.
authors.forename: Liqiu
authors.initials: L
authors.lastname: Xia
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology-State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, China. Electronic address: hsbalp@163.com.
authors.forename: Shengbiao
authors.initials: S
authors.lastname: Hu
mesh_terms: D000898:Antibiosis; D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D000935:Antifungal Agents / Q000378:metabolism*; D001688:Biological Products / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D020125:Mutation, Missense; D011549:Pseudomonas / Q000187:drug effects / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology*; D064210:Secondary Metabolism
chemical_list: D000904:Antibiotics, Antitubercular; D000935:Antifungal Agents; D001688:Biological Products; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin
keywords: Antifungal activity; Pseudomonas protegens; Ribosome engineering; Rifampicin resistance; Secondary metabolite