title: Hyperbaric oxygen therapy stimulates colonic stem cells and induces mucosal healing in patients with refractory ulcerative colitis: a prospective case series.
abstract: BACKGROUND
  Hyperbaric oxygen (HBO) is used as part of treatment in a variety of clinical conditions. Its use in the treatment of ulcerative colitis has been reported in few clinical reports.
  
  
  OBJECTIVE
  We report the effect of HBO on refractory ulcerative colitis exploring one potential mechanism of action.
  
  
  METHODS
  A review of records of patients with refractory ulcerative colitis who received HBO was conducted. Clinical and histopathological scoring was utilised to evaluate the response to HBO therapy (HBOT).
  
  
  RESULTS
  All patients manifested clinical improvement by the 40th cycle of HBOT. The median number of stool frequency dropped from seven motions/day (range=3-20) to 1/day (range=0.5-3), which was significant (z=-4.6, p<0.001). None of the patients manifested persistent blood passage after HBOT (z=-3.2, p=0.002). The severity index significantly improved after HBOT (z=-4.97, p<0.001). Histologically, a significant reduction of the scores of activity was recorded accompanied by a significant increase in the proliferating cell nuclear antigen labelling index of the CD44 cells of the colonic mucosa (p=0.001).
  
  
  CONCLUSIONS
  HBOT is effective in the setting of refractory ulcerative colitis. The described protocol is necessary for successful treatment. HBOT stimulates colonic stem cells to promote healing.
authors.affiliation: Department of Surgery, El Kabbary General Hospital, Alexandria, Egypt; Department of Surgery, Faculty of Medicine, Alexandria Main University Hospital, Alexandria, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bekheit
authors.affiliation: Department of Pathology, Faculty of Medicine , Alexandria Main University Hospital , Alexandria , Egypt.
authors.forename: Nahed
authors.initials: N
authors.lastname: Baddour
authors.affiliation: Department of Surgery, Faculty of Medicine , Alexandria Main University Hospital , Alexandria , Egypt.
authors.forename: Khaled
authors.initials: K
authors.lastname: Katri
authors.affiliation: Department of Internal Medicine, Faculty of Medicine , Alexandria Main University Hospital , Alexandria , Egypt.
authors.forename: Yousry
authors.initials: Y
authors.lastname: Taher
authors.affiliation: Department of Hyperbaric Medicine , Naval Hospital , Alexandria , Egypt.
authors.forename: Khaled
authors.initials: K
authors.lastname: El Tobgy
authors.affiliation: Department of Surgery, Faculty of Medicine , Alexandria Main University Hospital , Alexandria , Egypt.
authors.forename: Essam
authors.initials: E
authors.lastname: Mousa
keywords: CHRONIC ULCERATIVE COLITIS; GASTROINTESTINAL BLEEDING; INFLAMMATORY BOWEL DISEASE; ULCERATIVE COLITIS