title: Reversal of Multidrug Resistance and Computational Studies of Pistagremic Acid Isolated from Pistacia integerrima.
abstract: Pistagremic acid (PA) is a bioactive triterpenoid isolated from various parts of Pistacia integerrima plants. The aim of this research was to investigate PA for reversion of multidrug resistant (MDR) mediated by P-glycoprotein using rhodamine-123 exclusion study on a multidrug resistant human ABCB1 (ATP-binding cassette, sub-family B, member 1) gene-transfected mouse T-lymphoma cell line in vitro. Results were similar to those with verapamil as a positive control. Docking studies of PA and standard Rhodamine123 were carried out against a P-gp crystal structure which showed satisfactory results. Actually, PA cannot bind exactly where co-crystallized ligand of P-gp is already present. However, the docking study predicted that if a compound gives a lesser score then it may have some potency. The docking scores of PA and Rhodamine were similar. Therefore, we can conclude that there are certain important chemical features of PA which are responsible for the inhibiting potency of P-gp.
authors.affiliation: Department of Geology, University of Swabi, Anbar-23561, Khyber Pakhtunkhwa, Pakistan E-mail : mashaljcs@yahoo.com.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.forename: Ghias
authors.initials: G
authors.lastname: Uddin
authors.forename: Muslim
authors.initials: M
authors.lastname: Raza
authors.forename: Aftab
authors.initials: A
authors.lastname: Ahmad
authors.forename: Noor
authors.initials: N
authors.lastname: Jehan
authors.forename: Bashir
authors.initials: B
authors.lastname: Ahmad
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nisar
authors.forename: Joseph
authors.initials: J
authors.lastname: Molnar
authors.forename: Akos
authors.initials: A
authors.lastname: Csonka
authors.forename: Diana
authors.initials: D
authors.lastname: Szabo
authors.forename: Ajmal
authors.initials: A
authors.lastname: Khan
authors.forename: Umar
authors.initials: U
authors.lastname: Farooq
authors.forename: Mah
authors.initials: M
authors.lastname: Noor
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics / Q000378:metabolism; D000818:Animals; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D016399:Lymphoma, T-Cell / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D015394:Molecular Structure; D027927:Pistacia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D010936:Plant Extracts; D014315:Triterpenes; C581170:pistagremic acid