title: Global transcriptomic profiling in barramundi (Lates calcarifer) from rivers impacted by differing agricultural land uses.
abstract: Most catchments discharging into the Great Barrier Reef lagoon have elevated loads of suspended sediment, nutrients, and pesticides, including photosystem II inhibiting herbicides, associated with upstream agricultural land use. To investigate potential impacts of declining water quality on fish physiology, RNA sequencing (RNASeq) was used to characterize and compare the hepatic transcriptomes of barramundi (Lates calcarifer) captured from 2 of these tropical river catchments in Queensland, Australia. The Daintree and Tully Rivers differ in upstream land uses, as well as sediment, nutrient, and pesticide loads, with the area of agricultural land use and contaminant loads lower in the Daintree. In fish collected from the Tully River, transcripts involved in fatty acid metabolism, amino acid metabolism, and citrate cycling were also more abundant, suggesting elevated circulating cortisol concentrations, whereas transcripts involved in immune responses were less abundant. Fish from the Tully also had an increased abundance of transcripts associated with xenobiotic metabolism. Previous laboratory-based studies observed similar patterns in fish and amphibians exposed to the agricultural herbicide atrazine. If these transcriptomic patterns are manifested at the whole organism level, the differences in water quality between the 2 rivers may alter fish growth and fitness. Environ Toxicol Chem 2017;36:103-112. © 2016 SETAC.
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Kirrawee, New South Wales, Australia.
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Hook
authors.affiliation: Australian Institute of Marine Science, Townsville, Queensland, Australia.
authors.forename: Frederieke J
authors.initials: FJ
authors.lastname: Kroon
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Dutton Park, Queensland, Australia.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Metcalfe
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), North Ryde, New South Wales, Australia.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Greenfield
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Acton, Australian Capital Territory, Australia.
authors.forename: Philippe
authors.initials: P
authors.lastname: Moncuquet
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Acton, Australian Capital Territory, Australia.
authors.forename: Annette
authors.initials: A
authors.lastname: McGrath
authors.affiliation: Queensland Department of Science, Information Technology, and Innovation, Brisbane, Queensland, Australia.
authors.forename: Rachael
authors.initials: R
authors.lastname: Smith
authors.affiliation: Queensland Department of Science, Information Technology, and Innovation, Brisbane, Queensland, Australia.
authors.forename: Michael St J
authors.initials: MS
authors.lastname: Warne
authors.affiliation: Queensland Department of Science, Information Technology, and Innovation, Brisbane, Queensland, Australia.
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Turner
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Smithfield, Queensland, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: McKeown
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Atherton, Queensland, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Westcott
mesh_terms: D000383:Agriculture; D000818:Animals; D004784:Environmental Monitoring / Q000379:methods*; D020869:Gene Expression Profiling; D008297:Male; D010473:Perciformes / Q000235:genetics*; D010575:Pesticides / Q000032:analysis / Q000633:toxicity*; D011793:Queensland; D045483:Rivers / Q000737:chemistry*; D059467:Transcriptome / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D010575:Pesticides; D014874:Water Pollutants, Chemical
keywords: Diuron; Gene expression; Imidacloprid; Pesticides; RNA-sequence