title: Pin p 1 is a major allergen in pine nut and the first food allergen described in the plant group of gymnosperms.
abstract: This study aimed to report the complete sequence of a 2S albumin purified from pine nut and to analyze its allergenic properties. Individual recognition of this protein by serum IgE from pine nut-allergic patients was assessed. IgE cross-linking capacity was analyzed in a basophil activation test. Inhibition of IgE-binding and stability to heating was also assessed. The complete nucleotide sequence was obtained and a phylogenetic study was carried out. 2S albumin from pine nut (registered as Pin p 1.0101) was recognized by IgE of 75% of sera. The allergen was heat-stable and had a robust capacity to inhibit IgE-binding to whole pine nut extract. The IgE cross-linking capacity of Pin p 1 on basophils was also demonstrated. Despite the low homology of Pin p 1 sequence with other allergenic 2S albumins from angiosperms, Pin p 1 contains the typical skeleton of 8 cysteine residues, important for its α-helixes enriched structure.
authors.affiliation: Department of Dermatology and Allergy, University of Bonn Medical Center, Sigmund-Freud-Str. 25, 53127 Bonn, Germany. Electronic address: Beatriz.Cabanillas@ukb.uni-bonn.de.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Cabanillas
authors.affiliation: Servicio de Alergia, Hospital Universitario 12 de Octubre, Instituto de Investigación Hospital 12 de Octubre (i+12), Avenida de Córdoba s/n, 28041 Madrid, Spain.
authors.forename: Jesus F
authors.initials: JF
authors.lastname: Crespo
authors.affiliation: U.S. Department of Agriculture, Agriculture Research Service, Southern Regional Research Center, 1100 Robert E. Lee Boulevard, New Orleans, LA, USA.
authors.forename: Soheila J
authors.initials: SJ
authors.lastname: Maleki
authors.affiliation: Servicio de Alergia, Hospital Universitario 12 de Octubre, Instituto de Investigación Hospital 12 de Octubre (i+12), Avenida de Córdoba s/n, 28041 Madrid, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Rodriguez
authors.affiliation: Department of Dermatology and Allergy, University of Bonn Medical Center, Sigmund-Freud-Str. 25, 53127 Bonn, Germany.
authors.forename: Natalija
authors.initials: N
authors.lastname: Novak
mesh_terms: D055516:2S Albumins, Plant / Q000235:genetics* / Q000378:metabolism; D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D000485:Allergens / Q000235:genetics* / Q000378:metabolism; D001491:Basophils; D032462:Cycadopsida; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D009754:Nuts / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D055516:2S Albumins, Plant; D000418:Albumins; D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E
keywords: Albumin; Basophil activation test; Pin p 1; Pine nut allergy; Tree nut allergy