title: Characterization and Screening of Native Scenedesmus sp. Isolates Suitable for Biofuel Feedstock.
abstract: In current study isolates of two native microalgae species were screened on the basis of growth kinetics and lipid accumulation potential. On the basis of data obtained on growth parameters and lipid accumulation, it is concluded that Scenedesmus dimorphus has better potential as biofuel feedstock. Two of the isolates of Scenedesmus dimorphus performed better than other isolates with respect to important growth parameters with lipid content of ~30% of dry biomass. Scenedesmus dimorphus was found to be more suitable as biodiesel feedstock candidate on the basis of cumulative occurrence of five important biodiesel fatty acids, relative occurrence of SFA (53.04%), MUFA (23.81%) and PUFA (19.69%), and more importantly that of oleic acid in its total lipids. The morphological observations using light and Scanning Electron Microscope and molecular characterization using amplified 18S rRNA gene sequences of microalgae species under study were also performed. Amplified 18S rRNA gene fragments of the microalgae species were sequenced, annotated at the NCBI website and phylogenetic analysis was done. We have published eight 18S rRNA gene sequences of microalgae species in NCBI GenBank.
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, Himachal Pradesh, India.
authors.forename: Rakesh Singh
authors.initials: RS
authors.lastname: Gour
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, Himachal Pradesh, India.
authors.forename: Aseem
authors.initials: A
authors.lastname: Chawla
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, Himachal Pradesh, India.
authors.forename: Harvinder
authors.initials: H
authors.lastname: Singh
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, Himachal Pradesh, India.
authors.forename: Rajinder Singh
authors.initials: RS
authors.lastname: Chauhan
authors.affiliation: Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, Himachal Pradesh, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kant
mesh_terms: D056804:Biofuels; D018533:Biomass; D004247:DNA / Q000737:chemistry; D017931:DNA Primers; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D007194:India; D007700:Kinetics; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000737:chemistry; D044444:Scenedesmus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D056804:Biofuels; D017931:DNA Primers; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D012337:RNA, Ribosomal, 18S; D004247:DNA