title: Collagen-Immobilized Lipases Show Good Activity and Reusability for Butyl Butyrate Synthesis.
abstract: Candida rugosa lipases were immobilized onto collagen fibers through glutaraldehyde cross-linking method. The immobilization process has been optimized. Under the optimal immobilization conditions, the activity of the collagen-immobilized lipase reached 340 U/g. The activity was recovered of 28.3 % by immobilization. The operational stability of the obtained collagen-immobilized lipase for hydrolysis of olive oil emulsion was determined. The collagen-immobilized lipase showed good tolerance to temperature and pH variations in comparison to free lipase. The collagen-immobilized lipase was also applied as biocatalyst for synthesis of butyl butyrate from butyric acid and 1-butanol in n-hexane. The conversion yield was 94 % at the optimal conditions. Of its initial activity, 64 % was retained after 5 cycles for synthesizing butyl butyrate in n-hexane.
authors.affiliation: The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, 610065, China.
authors.forename: Song
authors.initials: S
authors.lastname: Dewei
authors.affiliation: The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, 610065, China.|National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, 610065, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Min
authors.affiliation: The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, 610065, China. chenghaiming@scu.edu.cn.|National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, 610065, China. chenghaiming@scu.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Haiming
mesh_terms: D000818:Animals; D055162:Biocatalysis / Q000187:drug effects; D002087:Butyrates / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D002175:Candida / Q000201:enzymology; D002417:Cattle; D003094:Collagen / Q000494:pharmacology*; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification / Q000187:drug effects; D005976:Glutaral / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D016062:Porosity; D059027:Recycling; D013696:Temperature; D013997:Time Factors
chemical_list: D002087:Butyrates; D004800:Enzymes, Immobilized; C022793:n-butyl n-butyrate; D003094:Collagen; D008049:Lipase; D005976:Glutaral
keywords: Butyl butyrate; Collagen; Esterification; Glutaraldehyde; Immobilization; Lipase