title: Steroidal composition and cytotoxic activity from fruiting body of Cortinarius xiphidipus.
abstract: From the fruiting body of ectomycorrhizal fungi Cortinarius xiphidipus, sterols were identified from the crude extract and the cytotoxic effect of ergosta-4, 6, 8(14), 22-tetraen-3-one (ergone) was evaluated. Ten sterols including ergosta-3,5,7,9(11),22-pentaene, (22E)-ergosta-5,7,9(11),22-tetraen-3b-ol, (3β,22E)-ergosta-5,7,22-trien-3-ol, (22E)-ergosta-7,22-dien-3-ol, neoergosterol, (3β)-ergosta-5,8-dien-3-ol, (3β)-ergosta-7-en-3-ol, stigmasterol, stigmasterol 22,23-dihydro and (22E)-ergosta-4,6,8(14),22-tetraen-3-one were identified from the crude extract. The cytotoxic activity of the sterol fraction containing ergosta-4, 6, 8(14), 22-tetraen-3-one was assessed on four tumour cell lines (Neuro-2a, Saos-2, MCF7 and LNCaP-C42). The cytotoxic activity against the four tumour cell lines tested, being Neuro-2a and Saos-2 the most sensitive, with a half-maximal inhibitory concentration (IC50) of 20.8 ± 2.2 and 27.8 ± 1.0 μg/mL, respectively. This is the first report of this Antarctic fungi collected in the Magallanes and Chilean Antarctica Region. This work represents a potential source for the development of anticancer drugs.
authors.affiliation: a Laboratory of Natural Products Chemistry, Department of Botany , University of Concepción , Concepción , Chile.
authors.forename: Solange
authors.initials: S
authors.lastname: Torres
authors.affiliation: a Laboratory of Natural Products Chemistry, Department of Botany , University of Concepción , Concepción , Chile.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cajas
authors.affiliation: b Laboratory of Mycology and Mycorrhizal, Department of Botany , University of Concepción , Concepción , Chile.
authors.forename: Goetz
authors.initials: G
authors.lastname: Palfner
authors.affiliation: c Laboratory of Cellular Culturing and Marine Genomics, Marine Biotechnology Unit and COPAS Sur-Austral Program , University of Concepción , Concepción , Chile.
authors.forename: Allisson
authors.initials: A
authors.lastname: Astuya
authors.affiliation: c Laboratory of Cellular Culturing and Marine Genomics, Marine Biotechnology Unit and COPAS Sur-Austral Program , University of Concepción , Concepción , Chile.
authors.forename: Ambbar
authors.initials: A
authors.lastname: Aballay
authors.affiliation: a Laboratory of Natural Products Chemistry, Department of Botany , University of Concepción , Concepción , Chile.
authors.forename: Claudia
authors.initials: C
authors.lastname: Pérez
authors.affiliation: a Laboratory of Natural Products Chemistry, Department of Botany , University of Concepción , Concepción , Chile.
authors.forename: Víctor
authors.initials: V
authors.lastname: Hernández
authors.affiliation: a Laboratory of Natural Products Chemistry, Department of Botany , University of Concepción , Concepción , Chile.
authors.forename: José
authors.initials: J
authors.lastname: Becerra
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D055363:Cortinarius / Q000737:chemistry*; D004875:Ergosterol / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D013261:Sterols / Q000032:analysis*
chemical_list: D000970:Antineoplastic Agents; D013261:Sterols; C059438:ergosta-7,22-dien-3-ol; D004875:Ergosterol
keywords: Cortinarius xiphidipus; cytotoxicity; ergosterols; fruiting body; mycorrhizal fungus