title: Antioxidant and Anti-Inflammatory Effects of Coenzyme Q10 on L-Arginine-Induced Acute Pancreatitis in Rat.
abstract: This study was aimed at evaluating the protective effect of coenzyme Q10 on L-arginine-induced acute pancreatitis in rats regarding biomarkers and morphologic changes. Thirty-two male Sprague-Dawley rats were divided into 4 equal groups. Control group received intraperitoneal normal saline, while in sham and experimental groups 1 and 2 pancreatitis was induced with L-arginine. E1 and E2 groups were treated with a single dose of 100 and 200 mg/kg Q10, respectively. Serum lipase and amylase, along with pancreas IL-10, IL-1β, and TNF-α, were measured. For evaluation of oxidative stress, pancreatic superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and myeloperoxidase (MPO) were assessed. Histopathological examination for morphologic investigation was conducted. Serum amylase and lipase, as well as TNF-α and IL-1β cytokines, reverted with administration of Q10 in consistence with dosage. In contrast, Q10 assisted in boosting of IL-10 with higher dosage (200 mg/kg). A similar pattern for oxidative stress markers was noticed. Both MDA and MPO levels declined with increased dosage, contrary to elevation of SOD and GSH. Histopathology was in favor of protective effects of Q10. Our findings proved the amelioration of pancreatic injury by Q10, which suggest the anti-inflammatory and antioxidant property of Q10 and its potential therapeutic role.
authors.affiliation: Department of Surgery, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Seyed Abbas
authors.initials: SA
authors.lastname: Mirmalek
authors.affiliation: Students' Research Committee, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Ala
authors.initials: A
authors.lastname: Gholamrezaei Boushehrinejad
authors.affiliation: Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Yavari
authors.affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Kardeh
authors.affiliation: Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Yekta
authors.initials: Y
authors.lastname: Parsa
authors.affiliation: Students' Research Committee, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Salimi-Tabatabaee
authors.affiliation: Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Yadollah-Damavandi
authors.affiliation: Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Tina
authors.initials: T
authors.lastname: Parsa
authors.affiliation: Students' Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Shahverdi
authors.affiliation: Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
authors.forename: Ehsan
authors.identifier: 0000-0002-6249-6617
authors.initials: E
authors.lastname: Jangholi
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001120:Arginine; D016207:Cytokines / Q000097:blood; D008049:Lipase / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D010195:Pancreatitis / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D017207:Rats, Sprague-Dawley; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D014451:Ubiquinone; D001120:Arginine; D008049:Lipase; D000681:Amylases; C024989:coenzyme Q10