title: Resveratrol attenuated hydrogen peroxide-induced myocardial apoptosis by autophagic flux.
abstract: BACKGROUND
  Resveratrol is a Sirt-1-specific activator, which also exerts cardioprotective effects that regulate redox signalling during oxidative stress and autophagy during cardiovascular disease (CVD).
  
  
  OBJECTIVE
  This study investigated the protective effects of resveratrol against hydrogen peroxide-induced damage in cardiomyocytes.
  
  
  METHODS
  In this article, hydrogen peroxide-induced autophagy and apoptosis in H9c2 cardiomyoblasts were studied at an increasing concentration from 0 to 100 µM.
  
  
  RESULTS
  Resveratrol pretreatment with concentrations of 10, 20, and 50 µM inhibits autophagic apoptosis by increasing p-Akt and Bcl-2 protein levels in H9c2 cells. Interestingly, resveratrol treatment activates the Beclin-1, LC3, p62, and the lysosome-associated protein LAMP2a within 24 h of administration.
  
  
  CONCLUSIONS
  These results suggest that resveratrol-regulated autophagy may play a role in degrading damaged organelles in H9c2 cells rather than causing apoptosis, and this may be a possible mechanism by which resveratrol protects the heart during CVD.
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.|School of Chinese Medicine, China Medical University, Taichung, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan.
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Ting
authors.affiliation: Translation Research Core, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Hospitality Management, College of Agriculture, Tunghai University, Taichung, Taiwan.
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Department of Hospitality Management, College of Agriculture, Tunghai University, Taichung, Taiwan; 040770@thu.edu.tw.
authors.forename: Wan-Teng
authors.initials: WT
authors.lastname: Lin
keywords: apoptosis; autophagy; oxidative stress; resveratrol