title: Comparison of two fabricated aptasensors based on modified carbon paste/oleic acid and magnetic bar carbon paste/Fe3O4@oleic acid nanoparticle electrodes for tetracycline detection.
abstract: In this research, we have improved two aptasensors based on a modified carbon paste electrode (CPE) with oleic acid (OA), and a magnetic bar carbon paste electrode (MBCPE) with Fe3O4 magnetic nanoparticles and oleic acid (OA). After the immobilization process of anti-TET at the electrode surfaces, the aptasensors were named CPE/OA/anti-TET and MBCPE/Fe3O4NPs/OA/anti-TET respectively. In this paper, the detection of tetracycline is compared using CPE/OA/anti-TET and MBCPE/Fe3O4NPs/OA/anti-TET aptasensors. These modified electrodes were characterized by infrared spectroscopy (IR), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), UV-vis spectroscopy, and voltammetric methods. The linear range and the detection limit for TET with the CPE/OA/anti-TET aptasensor were found to be 1.0×10(-12)-1.0×10(-7)M and 3.0×10(-13)M respectively by EIS method. The linear range and the detection limit for TET with the CPE/OA/anti-TET aptasensor were found to be 1.0×10(-10)-1.0×10(-7)M with a limit of detection of 2.9×10(-11)M using differential pulse voltammetry (DPV) technique. The MBCPE/Fe3O4NPs/OA/anti-TET aptasensor was used for determination of TET, and a liner range of 1.0×10(-14)-1.0×10(-6)M with a detection limit of 3.8×10(-15)M was obtained by EIS method. Also, the linear range and detection limit of 1.0×10(-12)-1.0×10(-6)M and 3.1×10(-13)M respectively, were obtained for MBCPE/Fe3O4NPs/OA/anti-TET aptasensor using DPV. The proposed aptasensors were applied for determination of tetracycline in some real samples such as drug, milk, honey and blood serum samples.
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Islamic Republic Iran.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Jahanbani
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd, Islamic Republic Iran. Electronic address: abenvidi@yazd.ac.ir.
authors.forename: Ali
authors.initials: A
authors.lastname: Benvidi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D005504:Food Analysis / Q000379:methods; D006722:Honey / Q000032:analysis; D006801:Humans; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D019301:Oleic Acid / Q000737:chemistry*; D013752:Tetracycline / Q000032:analysis* / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D058185:Magnetite Nanoparticles; D019301:Oleic Acid; D002244:Carbon; D013752:Tetracycline
keywords: Aptamer; Electrochemical impedance spectroscopy; Fe(3)O(4) magnetic nanoparticles; Immobilization; Magnetic bar carbon paste electrode; Tetracycline