title: Structure and antioxidative property of a polysaccharide from an ammonium oxalate extract of Phellinus linteus.
abstract: In this paper, the novel polysaccharide PL-A11 was purified from an ammonium oxalate extract of Phellinus linteus mycelia. Its physicochemical properties, structural characteristics, and antioxidant activities were investigated. Results showed that PL-A11 had a weight-average molecular weight (Mw) of 13.8kDa and was mainly composed of arabinose, xylose, mannose, and glucose in a molar ratio of 1.1:1.3:1.0:6.6. The backbone of PL-A11 was composed of (1→4)-α-d-glucopyranosyl, (1→2)-α-d-xylopyranosyl, and (1→3)-α-d-arabinofuranosyl residues, whereas the (1→6)-α-d-mannopyranosyl residues formed branches at the O-2 position with 1-linked-α-d-glucopyranosyl terminal residues. From the antioxidative activity tests in vivo, the administration of PL-A11 obviously enhanced the activity of antioxidant enzymes and significantly reduced the level of malondiadehyde (MDA) in the serum and liver of d-galactose-treated aging mice in a dose-dependent manner, as well as effectively stimulated the immune system of aging mice. These findings implied that PL-A11 could be developed as a potential antioxidant for applications in the functional food, pharmaceutical, cosmetic or nutraceutical industries.
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China. Electronic address: jkyan_27@163.com.
authors.forename: Jing-Kun
authors.initials: JK
authors.lastname: Yan
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Yao-Yao
authors.initials: YY
authors.lastname: Wang
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Zhen-Bin
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Hai-Le
authors.initials: HL
authors.lastname: Ma
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Pei
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Electronic address: jian-yong.wu@polyu.edu.hk.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Wu
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D019815:Oxalic Acid / Q000737:chemistry*; D000084682:Phellinus; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014781:Viscera / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009005:Monosaccharides; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D008315:Malondialdehyde; D019815:Oxalic Acid
keywords: Antioxidative activity; Phellinus linteus polysaccharide; Structural characterization