title: Urinary metabolomic profiling of asthmatics can be related to clinical characteristics.
abstract: Metabolomics has been increasingly explored to achieve an improved understanding of asthma. In the current observational and exploratory study, the first to have examined the relationship between oxidative stress extension, eosinophilic inflammation, and disease severity in asthmatic patients, metabolomics (using target aliphatic aldehydes and alkanes) was carried out using solid-phase microextraction (SPME) followed by a comprehensive two-dimensional gas chromatography coupled to mass spectrometry with a high-resolution time-of-flight analyzer (GC×GC-ToFMS). We were able to demonstrate that metabolomics can give valuable insights into asthma mechanisms once lipidic peroxidation assessed by urinary metabolomics is related to the clinical characteristics of nonobese asthmatics, such as disease severity, lung function, and eosinophilic inflammation. Nevertheless, considering our sample size, the obtained results require further validation using a much larger sample cohort.
authors.affiliation: Pneumology Unit, Hospitais da Universidade de Coimbra, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal.|Centre of Pneumology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
authors.forename: C C
authors.identifier: 0000-0003-0438-6126
authors.initials: CC
authors.lastname: Loureiro
authors.affiliation: QOPNA and Departamento de Química, Universidade de Aveiro, Aveiro, Portugal.
authors.forename: A S
authors.initials: AS
authors.lastname: Oliveira
authors.affiliation: QOPNA and Departamento de Química, Universidade de Aveiro, Aveiro, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.affiliation: CESAM and Departamento de Química, Universidade de Aveiro, Aveiro, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Rudnitskaya
authors.affiliation: Centre of Pneumology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.|Immunoallergology Unit, Hospitais da Universidade de Coimbra, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Todo-Bom
authors.affiliation: University Hospital Arnaud de Villeneuve, Montpellier, France.|CESP Centre for research in Epidemiology and Population Health, U1018, Respiratory and Environmental Epidemiology Team, Inserm, Villejuif, France.
authors.forename: J
authors.initials: J
authors.lastname: Bousquet
authors.affiliation: QOPNA and Departamento de Química, Universidade de Aveiro, Aveiro, Portugal.
authors.forename: S M
authors.initials: SM
authors.lastname: Rocha
mesh_terms: D000328:Adult; D001249:Asthma / Q000175:diagnosis* / Q000209:etiology / Q000652:urine*; D015415:Biomarkers; D004804:Eosinophils; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D015227:Lipid Peroxidation; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D010641:Phenotype; D012129:Respiratory Function Tests
chemical_list: D015415:Biomarkers; D007073:Immunoglobulin E
keywords: asthma; endotypes; metabolomics; oxidative stress