title: [Nanog promotes the invasion of breast cancer cells by increasing PKCε expression].
abstract: OBJECTIVE
  To study the relationship between Nanog-promoted metastasis of breast cancer and ezrin(T567) phosphorylation, and explore the possible mechanism by which Nanog regulates ezrin(T567) phosphorylation.
  
  
  METHODS
  A siRNA construct targeting Nanog was transfected in breast cancer cells to knock down Nanog expression, and the changes in the cell invasion was detected using Transwell assay. The expression levels of Nanog and PKC and the phosphorylation level of ezrin(T567) were detected using Western blotting and immunofluorescent staining; the protein interaction between PKCε and ezrin was assayed by co-immunoprecipitation and Western blotting.
  
  
  RESULTS
  Nanog knockdown significantly decreased the expression of PKCε protein, phosphorylation level of ezrin(T567) and the invasion ability of breast cancer cells. PKCε knockdown obviously decreased the phosphorylation level of ezrin(T567) in the cells, and PKCε and ezrin were co-immunoprecipitated.
  
  
  CONCLUSIONS
  Nanogcan can upregulate the expression of PKCε to promote the phosphorylation of ezrin(T567), which can be a new mechanism by which Nanog promotes tumor metastasis.
authors.affiliation: Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.E-mail: wf_shao @163.com.
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Shao
authors.forename: Fu-Xi
authors.initials: FX
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Tang
authors.forename: Xiao-Ran
authors.initials: XR
authors.lastname: Yu
authors.forename: Qiao-Sheng
authors.initials: QS
authors.lastname: Wen
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Yu
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Xiong
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D000071317:Nanog Homeobox Protein; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D051744:Protein Kinase C-epsilon / Q000378:metabolism*; D034741:RNA, Small Interfering; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; D051744:Protein Kinase C-epsilon; D034741:RNA, Small Interfering; D000099149:Ezrin; C486780:NANOG protein, human; C497255:PRKCE protein, human