title: Prediction and validation of cis-regulatory elements in 5' upstream regulatory regions of lectin receptor-like kinase gene family in rice.
abstract: Lectin receptor-like kinases (LecRLKs) play crucial roles in regulating plant growth and developmental processes in response to stress. In transcriptional gene regulation for normal cellular functions, cis-acting regulatory elements (CREs) direct the temporal and spatial gene expression with respect to environmental stimuli. A complete insightful of the transcriptional gene regulation system relies on effective functional analysis of CREs. Here, we analyzed the potential putative CREs present in the promoters of rice LecRLKs genes by using PlantCARE database. The CREs in LecRLKs promoters are associated with plant growth/development, light response, plant hormonal regulation processes, various stress responses, hormonal response like ABA, root-specific expression responsive, drought responsive, and cell and organ specific regulatory elements. The effect of methylation on these cis-regulatory elements was also analyzed. Real-time analysis of rice seedling under various stress conditions showed the expression levels of selected LecRLK genes superimposing the number of different CREs present in 5' upstream region. The overall results showed that the possible CREs function in the selective expression/regulation of LecRLKs gene family and during rice plant development under stress.
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, ArunaAsaf Ali Marg, New Delhi, 110067, India.
authors.forename: Nishat
authors.initials: N
authors.lastname: Passricha
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, ArunaAsaf Ali Marg, New Delhi, 110067, India.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Saifi
authors.affiliation: Zakir Husain Delhi College, University of Delhi, Jawahar Lal Nehru Marg, New Delhi, 110002, India.
authors.forename: Mohammad W
authors.initials: MW
authors.lastname: Ansari
authors.affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, ArunaAsaf Ali Marg, New Delhi, 110067, India. narendra@icgeb.res.in.|Amity Institute of Microbial Technology, Amity University, Noida, 201313, India. narendra@icgeb.res.in.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D001483:Base Sequence; D003198:Computer Simulation; D018899:CpG Islands / Q000235:genetics; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant; D008027:Light; D008954:Models, Biological; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D063245:Plant Development / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D011975:Receptors, Mitogen / Q000235:genetics* / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D011975:Receptors, Mitogen; D011494:Protein Kinases
keywords: Abiotic and biotic stress; LecRLKs; Promoter; Rice; cis-acting regulatory elements