title: High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system.
abstract: Quantitative proteomic approaches using selected reaction monitoring (SRM) are currently limited by the difficulty in the preparation of reference standards. In this study, we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to develop a large-scale SRM assay for targeted proteomics.
authors.affiliation: Division of Proteomics Research, Proteo-Science Center, Ehime University, Ehime, Japan. takemori@m.ehime-u.ac.jp.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Takemori
authors.affiliation: Division of Proteomics Research, Proteo-Science Center, Ehime University, Ehime, Japan. takemori@m.ehime-u.ac.jp.
authors.forename: Ayako
authors.initials: A
authors.lastname: Takemori
authors.affiliation: Advanced Research Support Center, Ehime University, Ehime, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Hematology, Clinical Immunology and Infectious Diseases, Ehime University Graduate School of Medicine, Ehime, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ishizaki
authors.affiliation: Department of Hematology, Clinical Immunology and Infectious Diseases, Ehime University Graduate School of Medicine, Ehime, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: Department of Ophthalmology, Ehime University Graduate School of Medicine, Ehime, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shiraishi
authors.affiliation: Department of Ophthalmology, Ehime University Graduate School of Medicine, Ehime, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohashi
mesh_terms: D002474:Cell-Free System; D057166:High-Throughput Screening Assays; D007553:Isotope Labeling; D019151:Peptide Library; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000378:metabolism*
chemical_list: D019151:Peptide Library