title: In Vivo Bone-Targeting of Bis(phosphonate)-Conjugated Double Helical RNA Monitored by Positron Emission Tomography.
abstract: A bis(phosphonate) conjugate of 2'-O-methyl oligoribonucleotide (microRNA-21) was synthesized and used as a bone-targeting carrier in the systemic delivery of a (68)Ga-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA)-chelated 2'-O-methyl oligoribonucleotide (anti-microRNA-21). The whole-body biodistribution of the double helical RNA was monitored by positron emission tomography (PET), which verified the expected bis(phosphonate)-induced bone accumulation in healthy rats.
authors.affiliation: Department of Chemistry, University of Turku , FI-20014 Turku, Finland.
authors.forename: Satish
authors.initials: S
authors.lastname: Jadhav
authors.affiliation: Turku PET Centre, University of Turku , FI-20521 Turku, Finland.|Turku PET Centre, Turku University Hospital , FI-20521 Turku, Finland.
authors.forename: Meeri
authors.initials: M
authors.lastname: Käkelä
authors.affiliation: Department of Cell Biology and Anatomy, University of Turku , FI-20520 Turku, Finland.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Bourgery
authors.affiliation: Department of Chemistry, University of Turku , FI-20014 Turku, Finland.
authors.forename: Kiira
authors.initials: K
authors.lastname: Rimpilä
authors.affiliation: Turku PET Centre, University of Turku , FI-20521 Turku, Finland.|Turku PET Centre, Turku University Hospital , FI-20521 Turku, Finland.|Turku Center for Disease Modeling, University of Turku , FI-20520 Turku, Finland.
authors.forename: Heidi
authors.initials: H
authors.lastname: Liljenbäck
authors.affiliation: Turku PET Centre, University of Turku , FI-20521 Turku, Finland.|Turku PET Centre, Turku University Hospital , FI-20521 Turku, Finland.
authors.forename: Riikka
authors.initials: R
authors.lastname: Siitonen
authors.affiliation: Turku PET Centre, University of Turku , FI-20521 Turku, Finland.|Turku PET Centre, Turku University Hospital , FI-20521 Turku, Finland.|Department of Cell Biology and Anatomy, University of Turku , FI-20520 Turku, Finland.
authors.forename: Jussi
authors.initials: J
authors.lastname: Mäkilä
authors.affiliation: Department of Cell Biology and Anatomy, University of Turku , FI-20520 Turku, Finland.
authors.forename: Tiina
authors.initials: T
authors.lastname: Laitala-Leinonen
authors.affiliation: Department of Chemistry, University of Turku , FI-20014 Turku, Finland.
authors.forename: Päivi
authors.initials: P
authors.lastname: Poijärvi-Virta
authors.affiliation: Department of Chemistry, University of Turku , FI-20014 Turku, Finland.
authors.forename: Harri
authors.initials: H
authors.lastname: Lönnberg
authors.affiliation: Turku PET Centre, University of Turku , FI-20521 Turku, Finland.|Turku PET Centre, Turku University Hospital , FI-20521 Turku, Finland.|Turku Center for Disease Modeling, University of Turku , FI-20520 Turku, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Roivainen
authors.affiliation: Department of Chemistry, University of Turku , FI-20014 Turku, Finland.
authors.forename: Pasi
authors.initials: P
authors.lastname: Virta
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000737:chemistry; D005710:Gallium Radioisotopes / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000737:chemistry; D008297:Male; D049268:Positron-Emission Tomography / Q000379:methods*; D012313:RNA / Q000032:analysis*; D034741:RNA, Small Interfering / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002614:Chelating Agents; D005710:Gallium Radioisotopes; D006571:Heterocyclic Compounds; D034741:RNA, Small Interfering; C075712:1,4,7-triazacyclononane; D012313:RNA
keywords: 68Ga-labeled oligonucleotides; bis(phosphonate); bone targeting; in vivo PET imaging; siRNA