title: Engineering the Campylobacter jejuni N-glycan to create an effective chicken vaccine.
abstract: Campylobacter jejuni is a predominant cause of human gastroenteritis worldwide. Source-attribution studies indicate that chickens are the main reservoir for infection, thus elimination of C. jejuni from poultry would significantly reduce the burden of human disease. We constructed glycoconjugate vaccines combining the conserved C. jejuni N-glycan with a protein carrier, GlycoTag, or fused to the Escherichia coli lipopolysaccharide-core. Vaccination of chickens with the protein-based or E. coli-displayed glycoconjugate showed up to 10-log reduction in C. jejuni colonization and induced N-glycan-specific IgY responses. Moreover, the live E. coli vaccine was cleared prior to C. jejuni challenge and no selection for resistant campylobacter variants was observed. Analyses of the chicken gut communities revealed that the live vaccine did not alter the composition or complexity of the microbiome, thus representing an effective and low-cost strategy to reduce C. jejuni in chickens and its subsequent entry into the food chain.
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Canada.|Alberta Glycomics Centre, University of Alberta, Edmonton, Canada.
authors.forename: Harald
authors.initials: H
authors.lastname: Nothaft
authors.affiliation: Delta Genomics, Edmonton, Canada.
authors.forename: Brandi
authors.initials: B
authors.lastname: Davis
authors.affiliation: Delta Genomics, Edmonton, Canada.
authors.forename: Yee Ying
authors.initials: YY
authors.lastname: Lock
authors.affiliation: Department of Agricultural, Food &Nutritional Science, University of Alberta, Edmonton, Canada.
authors.forename: Maria Elisa
authors.initials: ME
authors.lastname: Perez-Munoz
authors.affiliation: Human Health Therapeutics, National Research Council, Ottawa, Canada.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Canada.|Department of Agricultural, Food &Nutritional Science, University of Alberta, Edmonton, Canada.
authors.forename: Jens
authors.initials: J
authors.lastname: Walter
authors.affiliation: Delta Genomics, Edmonton, Canada.
authors.forename: Colin
authors.initials: C
authors.lastname: Coros
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Canada.|Alberta Glycomics Centre, University of Alberta, Edmonton, Canada.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Szymanski
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D002169:Campylobacter Infections / Q000235:genetics / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D016123:Campylobacter jejuni / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D002645:Chickens / Q000276:immunology / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control
chemical_list: D001428:Bacterial Vaccines