title: Study on the influential factors of Cd(2+) on the earthworm Eisenia fetida in oxidative stress based on factor analysis approach.
abstract: When earthworms are exposed to pollutants, their antioxidant system will have responses immediately. Consequently earthworms are widely used to monitor various pollutants as a sensitive bio-indicator. However, there are a large number of indices associated with the oxidative stress response. Finding out the key monitoring indices in the stress process becomes a practical demand of the pollution monitoring and warning process. Factor analysis approach is a statistical method that uses a few factors to replace many original factors. This paper is aimed at analyzing and sorting factors related to Cd(2+) on the earthworm Eisenia fetida in oxidative stress. We studied two groups, the short-term test and the long-term test. The former test lasted for ten days, removing an earthworm every day for analysis; The latter test lasted for 30 days, taking out an earthworm every ten days. The Cd(2+) concentration was set at 0, 50, 100, 125, 250 and 500 mg kg(-1), post-clitellum segments of earthworms were chosen to determine SOD, POD, GPX, GST, CAT, VE, MDA and AChE. The results showed that in the short-term group, the main bioindicator associated with oxidative stress reaction was CAT at the exposure time of 1-3 days, at 4-5 days MDA, 6-7 days POD, and GST and GPX at 8th day, CAT at 9-10 days. While with the long-term test, the main bioindicator associated with oxidative stress reaction was GPX.
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: zhboshi@163.com.
authors.forename: Dongxing
authors.initials: D
authors.lastname: Zhou
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yucui
authors.initials: Y
authors.lastname: Ning
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Guangdong
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D005163:Factor Analysis, Statistical; D009835:Oligochaeta / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress; D012989:Soil Pollutants / Q000633:toxicity*
chemical_list: D012989:Soil Pollutants; D002104:Cadmium
keywords: Cd exposure; Earthworm; Factor analysis approach; Heavy metal; Oxidative stress reaction