title: Microsatellite primers for a species of South African everlasting daisy (Helichrysum odoratissimum; Gnaphalieae, Asteraceae).
abstract: OBJECTIVE
  Microsatellites were developed for the widespread Helichrysum odoratissimum (Asteraceae) to estimate gene flow across diploid populations and to test if gene flow occurs among other closely related lineages within this genus.
  
  
  RESULTS
  Ten primer pairs were developed and tested using populations across South Africa; however, only seven primer pairs were polymorphic for the target species. The seven polymorphic primers amplified di- and trinucleotide repeats with up to 16 alleles per locus among 125 diploid individuals used for analyses.
  
  
  CONCLUSIONS
  These markers can be used to estimate gene flow among populations of known ploidy level of H. odoratissimum to test evolutionary hypotheses. Furthermore, these markers amplify successfully in other Helichrysum species, including the other three taxonomic Group 4 species, and therefore can be used to inform taxonomic work on these species.
authors.affiliation: School of Animal, Plant, and Environmental Sciences, University of the Witwatersrand, Private Bag 3, WITS 2050 Johannesburg, South Africa.
authors.forename: Kelsey L
authors.initials: KL
authors.lastname: Glennon
authors.affiliation: School of Animal, Plant, and Environmental Sciences, University of the Witwatersrand, Private Bag 3, WITS 2050 Johannesburg, South Africa.
authors.forename: Glynis V
authors.initials: GV
authors.lastname: Cron
keywords: Asteraceae; Helichrysum odoratissimum; gene flow; polyploidy; southern Africa; speciation