title: CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization.
abstract: The CRISPR system holds much promise for successful genome engineering, but therapeutic, industrial, and research applications will place high demand on improving the specificity and efficiency of this tool. CT-Finder (http://bioinfolab.miamioh.edu/ct-finder) is a web service to help users design guide RNAs (gRNAs) optimized for specificity. CT-Finder accommodates the original single-gRNA Cas9 system and two specificity-enhancing paired-gRNA systems: Cas9 D10A nickases (Cas9n) and dimeric RNA-guided FokI nucleases (RFNs). Optimal target candidates can be chosen based on the minimization of predicted off-target effects. Graphical visualization of on-target and off-target sites in the genome is provided for target validation. Major model organisms are covered by this web service.
authors.affiliation: Department of Biology, Miami University, Oxford, Ohio, 45056, USA.
authors.forename: Houxiang
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Biology, Miami University, Oxford, Ohio, 45056, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Misel
authors.affiliation: Department of Biology, Miami University, Oxford, Ohio, 45056, USA.
authors.forename: Mitchell
authors.initials: M
authors.lastname: Graham
authors.affiliation: Department of Biology, Miami University, Oxford, Ohio, 45056, USA.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Robinson
authors.affiliation: Department of Biology, Miami University, Oxford, Ohio, 45056, USA.|Department of Software Engineering, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Chun
authors.initials: C
authors.lastname: Liang
mesh_terms: D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D000072669:Gene Editing; D018390:Gene Targeting; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics; D012984:Software; D014584:User-Computer Interface; D064878:Web Browser
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems