title: Discriminatory usefulness of pulsed-field gel electrophoresis and sequence-based typing in Legionella outbreaks.
abstract: To compare the discriminatory power of pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) in Legionella outbreaks for determining the infection source.
  
  
  
  Twenty-five investigations of Legionnaires' disease were analyzed by PFGE, SBT and Dresden monoclonal antibody.
  
  
  
  The results suggested that monoclonal antibody could reduce the number of Legionella isolates to be characterized by molecular methods. The epidemiological concordance PFGE-SBT was 100%, while the molecular concordance was 64%. Adjusted Wallace index (AW) showed that PFGE has better discriminatory power than SBT (AWSBT→PFGE = 0.767; AWPFGE→SBT = 1). The discrepancies appeared mostly in sequence type (ST) 1, a worldwide distributed ST for which PFGE discriminated different profiles.
  
  
  
  SBT discriminatory power was not sufficient verifying the infection source, especially in worldwide distributed STs, which were classified into different PFGE patterns.
authors.affiliation: Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916.|Universitat Autònoma de Barcelona, Cerdanyola, Spain E-08193.
authors.forename: Sara
authors.initials: S
authors.lastname: Quero
authors.affiliation: Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916.|Universitat Autònoma de Barcelona, Cerdanyola, Spain E-08193.|CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Marian
authors.initials: M
authors.lastname: García-Núñez
authors.affiliation: Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916.|Universitat Autònoma de Barcelona, Cerdanyola, Spain E-08193.|CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Noemí
authors.initials: N
authors.lastname: Párraga-Niño
authors.affiliation: Vigilancia Epidemiologica, Agencia de Salut Publica de Catalunya, Barcelona, Spain E-08005.
authors.forename: Irene
authors.initials: I
authors.lastname: Barrabeig
authors.affiliation: Universitat Autònoma de Barcelona, Cerdanyola, Spain E-08193.|CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.|Unitat de Malalties Infeccioses, Hospital Universitari Germans Trias i Pujol, Badalona, Spain E-08916.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Pedro-Botet
authors.affiliation: Laboratori de l'Agència de Salut Pública de Barcelona, Barcelona, Spain E-08001.
authors.forename: Mercè
authors.initials: M
authors.lastname: de Simon
authors.affiliation: Universitat Autònoma de Barcelona, Cerdanyola, Spain E-08193.|CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.|Unitat de Malalties Infeccioses, Hospital Universitari Germans Trias i Pujol, Badalona, Spain E-08916.
authors.forename: Nieves
authors.initials: N
authors.lastname: Sopena
authors.affiliation: Universitat Autònoma de Barcelona, Cerdanyola, Spain E-08193.|CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.|Unitat de Malalties Infeccioses, Hospital Universitari Germans Trias i Pujol, Badalona, Spain E-08916.
authors.forename: Miquel
authors.initials: M
authors.lastname: Sabrià
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D007875:Legionella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013030:Spain / Q000453:epidemiology
keywords: Legionella; PFGE; SBT; congruence; discriminatory power; molecular-epidemiology outbreaks; typing