title: Regulation of gill claudin paralogs by salinity, cortisol and prolactin in Mozambique tilapia (Oreochromis mossambicus).
abstract: In euryhaline teleosts, reorganization of gill tight junctions during salinity acclimation involves dynamic expression of specific claudin (Cldn) paralogs. We identified four transcripts encoding Cldn tight junction proteins in the tilapia gill transcriptome: cldn10c, cldn10e, cldn28a and cldn30. A tissue distribution experiment found cldn10c and cldn10e expression levels in the gill to be 100-fold higher than any other tissues examined. cldn28a and cldn30 levels in the gill were 10-fold greater than levels in other tissues. Expression of these genes in Mozambique tilapia was examined during acclimation to fresh water (FW), seawater (SW), and in response to hormone treatments. Transfer of tilapia from FW to SW elevated cldn10c and cldn10e, while cldn28a and cldn30 were stimulated following transfer from SW to FW. In hypophysectomized tilapia transferred to FW, pituitary extirpation induced reduced expression of cldn10c, cldn10e and cldn28a; these effects were mitigated equally by either prolactin or cortisol replacement. In vitro experiments with gill filaments showed that cortisol stimulated expression of all four cldns examined, suggesting a direct action of cortisol in situ. Our data indicate that elevated cldn10c and cldn10e expression is important during acclimation of tilapia to SW possibly by conferring ion specific paracellular permeability. On the other hand, expression of cldn28a and cldn30 appears to contribute to reorganization of branchial epithelium during FW acclimation. Hormone treatment experiments showed that particular FW- and SW-induced cldns are controlled by cortisol and prolactin.
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA; Hawai'i Institute of Marine Biology, University of Hawai'i, Kāne'ohe, HI, USA. Electronic address: tipsmark@uark.edu.
authors.forename: Christian K
authors.initials: CK
authors.lastname: Tipsmark
authors.affiliation: Hawai'i Institute of Marine Biology, University of Hawai'i, Kāne'ohe, HI, USA; Department of Biology, Skidmore College, Saratoga Springs, NY, USA.
authors.forename: Jason P
authors.initials: JP
authors.lastname: Breves
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
authors.forename: D Brett
authors.initials: DB
authors.lastname: Rabeneck
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
authors.forename: Rebecca T
authors.initials: RT
authors.lastname: Trubitt
authors.affiliation: Hawai'i Institute of Marine Biology, University of Hawai'i, Kāne'ohe, HI, USA; Sea Grant College Program, University of Hawai'i at Mānoa, HI, USA.
authors.forename: Darren T
authors.initials: DT
authors.lastname: Lerner
authors.affiliation: Hawai'i Institute of Marine Biology, University of Hawai'i, Kāne'ohe, HI, USA.
authors.forename: E Gordon
authors.initials: EG
authors.lastname: Grau
mesh_terms: D000818:Animals; D057167:Claudins / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics*; D005618:Fresh Water; D005786:Gene Expression Regulation / Q000187:drug effects; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D007016:Hypophysectomy; D066298:In Vitro Techniques; D011388:Prolactin / Q000494:pharmacology*; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054712:Salinity; D012623:Seawater; D017210:Tilapia / Q000235:genetics*; D059467:Transcriptome / Q000187:drug effects
chemical_list: D057167:Claudins; D029941:Fish Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011388:Prolactin; D006854:Hydrocortisone
keywords: Epithelia; Growth hormone; Hypophysectomy; Osmoregulation; Tight junctions