title: The mitochondrial genome of the egg-laying flatworm Aglaiogyrodactylus forficulatus (Platyhelminthes: Monogenoidea).
abstract: The rather species-poor oviparous gyrodactylids are restricted to South America. It was suggested that they have a basal position within the otherwise viviparous Gyrodactylidae. Accordingly, it was proposed that the species-rich viviparous gyrodactylids diversified and dispersed from there.
  
  
  
  The mitochondrial genome of Aglaiogyrodactylus forficulatus was bioinformatically assembled from next-generation illumina MiSeq sequencing reads, annotated, and compared to previously published mitochondrial genomes of other monogenoidean flatworm species.
  
  
  
  The mitochondrial genome of A. forficulatus consists of 14,371 bp with an average A + T content of 75.12 %. All expected 12 protein coding, 22 tRNA, and 2 rRNA genes were identified. Furthermore, there were two repetitive non-coding regions essentially consisting of 88 bp and 233 bp repeats, respectively. Maximum Likelihood analyses placed the mitochondrial genome of A. forficulatus in a well-supported clade together with the viviparous Gyrodactylidae species. The gene order differs in comparison to that of other monogenoidean species, with rearrangements mainly affecting tRNA genes. In comparison to Paragyrodactylus variegatus, four gene order rearrangements, i.e. three transpositions and one complex tandem-duplication-random-loss event, were detected.
  
  
  
  Mitochondrial genome sequence analyses support a basal position of the oviparous A. forficulatus within Gyrodactylidae, and a sister group relationship of the oviparous and viviparous forms.
authors.affiliation: Natural History Museum, University of Oslo, PO Box 1172, Blindern, 0318, Oslo, Norway. bachmann@nhm.uio.no.
authors.forename: Lutz
authors.initials: L
authors.lastname: Bachmann
authors.affiliation: Department of Tumor Biology, Institute for Cancer Research, Norwegian Radium, Hospital, Oslo University Hospital, PO Box 4950, Nydalen, 0424, Oslo, Norway.
authors.forename: Bastian
authors.initials: B
authors.lastname: Fromm
authors.affiliation: Laboratório de Ecologia Molecular e Parasitologia Evolutiva-LEMPE, Universidade, Federal do Paraná-UFPR, Curitiba, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Patella de Azambuja
authors.affiliation: Laboratório de Ecologia Molecular e Parasitologia Evolutiva-LEMPE, Universidade, Federal do Paraná-UFPR, Curitiba, Brazil.
authors.forename: Walter A
authors.initials: WA
authors.lastname: Boeger
mesh_terms: D000818:Animals; D002397:Catfishes / Q000469:parasitology; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D005260:Female; D005393:Fish Diseases / Q000469:parasitology; D023061:Gene Order; D054629:Genome, Mitochondrial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary; D008928:Mitochondria / Q000235:genetics; D010058:Oviposition; D010986:Platyhelminths / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
chemical_list: D004272:DNA, Mitochondrial
keywords: Brazil; Fish parasites; Gene order; Gyrodactylidae; Mitogenomics; Monogenoidea; Neodermata