title: Synthesis, structures, molecular docking, cytotoxicity and bioimaging studies of two novel Zn(II) complexes.
abstract: Two novel compounds [Zn2(Endc)2(bipy)2(H2O)3]·4(H2O)·2(O)(1), [Zn2(Endc)2(phen)2(H2O)]·(O)(2) (bipy = 2,2-bipyridine, phen = 1,10-phenanthroline, and Endc = endo-norbornene-cis-5,6-dicarboxylicacid) have been synthesized and characterized. In this paper abbreviations are FS-DNA (fish sperm DNA), HeLa (human cervix epithelia carcinoma cells), KB (human oral epithelial carcinoma cells), LO2 (human liver cell L-O2), EtBr (ethidium bromide), DMF (Dimethyl Formamide), MTT ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium]). The binding of complexes with Fish Sperm DNA were measured by electronic absorption spectra and fluorescence spectroscopy. The ability of these complexes to cleave the pBR322 plasmid DNA or the KB and HeLa DNA extracted in our laboratory was demonstrated by gel electrophoresis assay. The cytotoxic effects of these complexes were examined on two tumor cell lines, HeLa, KBr and one normal cell line LO-2. UV absorption and fluorescence spectra indicate the ability of the complexes bond to DNA with different binding affinity. Gel electrophoresis assay demonstrates which one complex more effective DNA-cleavage activity. The cytotoxic activity of the complexes was tested against two different cancer and one normal cell lines. The two complexes exhibited cytotoxic specificity and significant cancer cell inhibitory rate and lower cytotoxicity toward the normal cell lines. The unique interaction mode with DNA and cancer cells inhibition effect clearly revealed the relationship between the structure and the activity of the novel antitumor agent Zn(II) complexes.
authors.affiliation: The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, PR China. Electronic address: enjungao@163.com.
authors.forename: Enjun
authors.initials: E
authors.lastname: Gao
authors.affiliation: The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, PR China.
authors.forename: Shaozhong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, PR China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Ding
authors.affiliation: The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Qiu
authors.affiliation: The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, PR China. Electronic address: mczhu@syuct.edu.cn.
authors.forename: Mingchang
authors.initials: M
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002460:Cell Line; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D003603:Cytotoxins / Q000138:chemical synthesis / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects*; D006801:Humans; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship; D015032:Zinc / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D003603:Cytotoxins; D004247:DNA; D015032:Zinc
keywords: Apoptosis; Cytotoxicity; DNA binding; HeLa and KB cells