title: Simultaneous enantioselective quantification of fluoxetine and norfluoxetine in human milk by direct sample injection using 2-dimensional liquid chromatography-tandem mass spectrometry.
abstract: A two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2D LC-MS/MS) was employed for the simultaneously quantification of fluoxetine (FLX) and norfluoxetine (NFLX) enantiomers in human milk by direct injection of samples. A restricted access media of bovine serum albumin octadecyl column (RAM-BSAC18) was used in the first dimension for the milk proteins depletion, while an antibiotic-based chiral column was used in the second dimension. The results herein described show good selectivity, extraction efficiency, accuracy, and precision with limits of quantification in the order of 7.5ngmL(-1)for the FLX enantiomers and 10.0ngmL(-1) for NFLX enantiomers. Furthermore, it represents a practical tool in terms of sustainability for the sample preparation of such a difficult matrix.
authors.affiliation: SEPARARE-Núcleo de Pesquisa em Cromatografia, Departamento de Química, Universidade Federal de São Carlos, P.O. Box 676, São Carlos 13565-905, SP, Brazil.
authors.forename: Joel
authors.initials: J
authors.lastname: Alvim
authors.affiliation: SEPARARE-Núcleo de Pesquisa em Cromatografia, Departamento de Química, Universidade Federal de São Carlos, P.O. Box 676, São Carlos 13565-905, SP, Brazil.
authors.forename: Bianca Rebelo
authors.initials: BR
authors.lastname: Lopes
authors.affiliation: SEPARARE-Núcleo de Pesquisa em Cromatografia, Departamento de Química, Universidade Federal de São Carlos, P.O. Box 676, São Carlos 13565-905, SP, Brazil. Electronic address: quezia@pq.cnpq.br.
authors.forename: Quezia Bezerra
authors.initials: QB
authors.lastname: Cass
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008894:Milk Proteins / Q000302:isolation & purification; D008895:Milk, Human / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D008894:Milk Proteins; D005473:Fluoxetine; D012710:Serum Albumin, Bovine; C036139:norfluoxetine
keywords: Enantioselective quantification; Fluoxetine; Human milk; Restricted-access media; Tandem mass spectrometry; Two-dimensional liquid chromatography