title: Pseudoruegeria aestuarii sp. nov., of the family Rhodobacteraceae, isolated from a tidal flat.
abstract: A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain MME-001T, was isolated from the tidal flat of Muui-do in the Republic of Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain MME-001T belonged to the genus Pseudoruegeria in the family Rhodobacteraceae and that it shared the highest 16S rRNA gene sequence similarity with Pseudoruegeria sabulilitoris GJMS-35T (98.0 % similarity of the 16S rRNA gene). Growth of strain MME-001T occurred in the presence of 1.0-7.0 % (w/v) NaCl at 15-40 °C and pH 7.0-9.0, with optimal growth in the presence of 2.0-3.0 % (w/v) NaCl at 25-30 °C and pH 7.0. Ubiquinone-10 was the major respiratory quinone. Major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. The major cellular fatty acids were C18 : 1ω7c and C16 : 0. The genomic DNA G+C content was 62 mol%. DNA-DNA hybridization values between strain MME-001T and P. sabulilitoris KCTC 42111T, 'Pseudoruegeria limi' KCTC 32460, Pseudoruegeria lutimaris KCTC 22690T, Pseudoruegeria aquimaris KCTC 12737T and Pseudoruegeriahaliotis KACC 17214T was 36±5, 57±7, 34±4, 18±5 and 21±3 %, respectively. Based on the phenotypic and phylogenetic taxonomical properties, this strain MME-001T represents a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria aestuarii sp. nov. is proposed. The type strain is MME-001T (=KCCM 43133T=JCM 30751T).
authors.affiliation: Division of Bioengineering, Incheon National University, 22012 Incheon, Republic of Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Cha
authors.affiliation: Department of Life Sciences, Graduate School of Incheon National University, 22012 Incheon, Republic of Korea.
authors.forename: Inhye
authors.initials: I
authors.lastname: Park
authors.affiliation: Hygienic Safety and Analysis Center, World Institute of Kimchi, 61755 Gwangju, Republic of Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, Graduate School of Incheon National University, 22012 Incheon, Republic of Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Korean Culture Center of Microorganisms, 03641 Seoul, Republic of Korea.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Biological Disaster Analysis Group, Korea Basic Science Institute, 34133 Daejeon, Republic of Korea.
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, 61755 Gwangju, Republic of Korea.
authors.forename: Hak-Jong
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Research Group of Gut Microbiome, Korea Food Research Institute, 13539 Sungnam, Republic of Korea.
authors.forename: Young-Do
authors.initials: YD
authors.lastname: Nam
authors.affiliation: Korea Polar Research Institute, KIOST, 21990 Incheon, Republic of Korea.
authors.forename: Yoo Kyung
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, Graduate School of Incheon National University, 22012 Incheon, Republic of Korea.|Division of Bioengineering, Incheon National University, 22012 Incheon, Republic of Korea.
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2