title: A novel multi-epitope recombined protein for diagnosis of human brucellosis.
abstract: In epidemic regions of the world, brucellosis is a reemerging zoonosis with minimal mortality but is a serious public hygiene problem. Currently, there are various methods for brucellosis diagnosis, however few of them are available to be used to diagnose, especially for serious cross-reaction with other bacteria.
  
  
  
  To overcome this disadvantage, we explored a novel multi-epitope recombinant protein as human brucellosis diagnostic antigen. We established an indirect enzyme-linked immunosorbent assay (ELISA) based on this recombinant protein. 248 sera obtained from three different groups including patients with brucellosis (146 samples), non-brucellosis patients (82 samples), and healthy individuals (20 samples) were tested by indirect ELISA. To evaluate the assay, a receiver-operating characteristic (ROC) analysis and immunoblotting were carried out using these characterized serum samples.
  
  
  
  For this test, the area under the ROC curve was 0.9409 (95 % confidence interval, 0.9108 to 0.9709), and a sensitivity of 88.89 % and a specificity of 85.54 % was given with a cutoff value of 0.3865 from this ROC analysis. The Western blot results indicate that it is feasible to differentiate human brucellosis and non-brucellosis with the newly established method based on this recombinant protein.
  
  
  
  Our results obtained high diagnostic accuracy of the ELISA assay which encourage the use of this novel recombinant protein as diagnostic antigen to implement serological diagnosis of brucellosis.
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Dehui
authors.initials: D
authors.lastname: Yin
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.|Department of Infection Control, First Hospital of Jilin University, Changchun, China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ju
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Yushen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Hongqian
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China.|Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun, China.
authors.forename: Rizeng
authors.initials: R
authors.lastname: Meng
authors.affiliation: Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China. li_juan@jlu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China. xukun@jlu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
mesh_terms: D000942:Antigens, Bacterial / Q000097:blood* / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D002002:Brucella / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000097:blood / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D012372:ROC Curve; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial; D000939:Epitopes; D011994:Recombinant Proteins
keywords: Brucellosis; Diagnosis; Recombinant protein