title: De novo assembly and annotation of the marine mysid (Neomysis awatschensis) transcriptome.
abstract: We sequenced the whole transcriptome of the marine mysid Neomysis awatschensis (Crustacea: Mysidacea) using Illumina RNA-seq. De novo assembly was performed with 14,018,702 raw reads using Trinity, resulting in 82,434 contigs. Transdecoder found 22,141 candidate coding contigs with homology to other species by BLAST analysis. Functional gene annotation was performed by Gene Ontology, InterProScan, and KEGG pathway analyses. We generated an expressed gene catalog for the mysid N. awatschensis to serve as a resource for marine environmental genomic and ecotoxicogenomic studies focused on uncovering the molecular mechanisms underlying the responses of N. awatschensis to environmental stressors and chemicals.
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Hui-Su
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Dae-Sik
authors.initials: DS
authors.lastname: Hwang
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Lee
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Jun Chul
authors.initials: JC
authors.lastname: Park
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address: jslee2@skku.edu.
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D003445:Crustacea / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D017423:Sequence Analysis, RNA; D059467:Transcriptome
keywords: Ecotoxicogenomics; Mysid; Neomysis awatschensis; RNA-seq; Transcriptome