title: Novel degradation pathway and kinetic analysis for buprofezin removal by newly isolated Bacillus sp.
abstract: Given the intensive and widespread application of the pesticide, buprofezin, its environmental residues potentially pose a problem; yet little is known about buprofezin's kinetic and metabolic behaviors. In this study, a novel gram-positive strain, designated BF-5, isolated from aerobic activated sludge, was found to be capable of metabolizing buprofezin as its sole energy, carbon, and nitrogen source. Based on its physiological and biochemical characteristics, other aspects of its phenotype, and a phylogenetic analysis, strain BF-5 was identified as Bacillus sp. This study investigated the effect of culture conditions on bacterial growth and substrate degradation, such as pH, temperature, initial concentration, different nitrogen source, and additional nitrogen sources as co-substrates. The degradation rate parameters, qmax, Ks, Ki and Sm were determined to be 0.6918 h(-1), 105.4 mg L(-1), 210.5 mg L(-1), and 148.95 mg L(-1) respectively. The capture of unpublished potential metabolites by gas chromatography-mass spectrometry (GC-MS) analysis has led to the proposal of a novel degradation pathway. Taken together, our results clarify buprofezin's biodegradation pathway(s) and highlight the promising potential of strain BF-5 in bioremediation of buprofezin-contaminated environments.
authors.affiliation: College of Life Sciences, Huaibei Normal University, 235000, Huaibei, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Huaibei Normal University, 235000, Huaibei, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Huaibei Normal University, 235000, Huaibei, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Xiong
authors.affiliation: College of Life Sciences, Huaibei Normal University, 235000, Huaibei, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Huaibei Normal University, 235000, Huaibei, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Life Sciences, Huaibei Normal University, 235000, Huaibei, China. Electronic address: liuyuan0813@aliyun.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000378:metabolism; D007700:Kinetics; D053858:Metabolic Networks and Pathways; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology; D013696:Temperature; D013829:Thiadiazines / Q000378:metabolism*
chemical_list: D007306:Insecticides; D012722:Sewage; D013829:Thiadiazines; C083997:buprofezin; D002244:Carbon; D009584:Nitrogen
keywords: Bacillus sp. BF-5; Biodegradation pathway; Buprofezin; Environmental remediation; Kinetics