title: Establishment and evaluation of a mouse model of bronchial asthma with Yin deficiency syndrome.
abstract: OBJECTIVE
  To establish and evaluate a mouse model of bronchial asthma with Yin deficiency syndrome.
  
  
  METHODS
  The mouse model of bronchial asthma with Yin deficiency syndrome was established by the treatment with injecting ovalbumin (OVA) two times to sensitize, inhaling OVA 14 times to stimulate, and using thyroxin through lavage during late stimulation. This model was evaluated through body weight, asthmatic behaviors, respiratory function, autonomous activity, lung pathology, and pulmonary fluid clearance.
  
  
  RESULTS
  OVA combined with thyroxin was an appropriate method to induce the mouse model with increased food and water intake, autonomous activity, asthmatic behaviors score, and respiratory rate, decreased body weight, tidal volume, and wet/dry ratio of lung, and changed with pathology of lung tissue. The changes of the above mentioned parameters indicated that the model was the bronchial asthma with Yin deficiency syndrome.
  
  
  CONCLUSIONS
  The OVA combined with thyroxin is a good pattern to establish a mouse model of bronchial asthma with Yin deficiency syndrome successfully, which can highly simulate the clinical symptoms of this disease.
authors.forename: Zhi-wang
authors.initials: ZW
authors.lastname: Wang
authors.forename: Rong-ke
authors.initials: RK
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ren
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Liu
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Cheng
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Tuo
mesh_terms: D000818:Animals; D001249:Asthma / Q000503:physiopathology*; D001980:Bronchi / Q000503:physiopathology; D004195:Disease Models, Animal; D051379:Mice; D010047:Ovalbumin; D013974:Thyroxine; D016710:Yin Deficiency
chemical_list: D010047:Ovalbumin; D013974:Thyroxine