title: EPR spectroscopy solutions for assessment of decellularization of intrathoracic organs and tissues.
abstract: Using EPR spectroscopy it was established that the determination of the concentration of paramagnetic centers in lyophilized tissues allows indirect evaluation of the quality of decellularization of intrathoracic organs (diaphragm, heart, and lungs), since the content of paramagnetic particles in them can serve as a criterion of cell viability and points to the necessity to repeat decellularization. Experiments in rats showed that the EPR spectra of the native thoracic organs contained paramagnetic centers with g-factor values ranging from 2.007 to 2.011 at a concentration of 10(-8) to 6.62 × 10(-7) mol/g of lyophilized tissue, whereas in all decellularized tissues of the same organs paramagnetic particles were not detected.
authors.affiliation: Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia. g_lena82@list.ru.
authors.forename: E A
authors.initials: EA
authors.lastname: Gubareva
authors.affiliation: Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Kuevda
authors.affiliation: Kuban State University, ul. Stavropol'skaya 149, Krasnodar, 350040, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Dzhimak
authors.affiliation: Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Basov
authors.affiliation: Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Sotnichenko
authors.affiliation: Kuban State University, ul. Stavropol'skaya 149, Krasnodar, 350040, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Bolotin
authors.affiliation: Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Gilevich
authors.affiliation: Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
authors.forename: I S
authors.initials: IS
authors.lastname: Gumenyuk
authors.affiliation: Kuban State Medical University, Ministry of Healthcare of the Russian Federation, ul. Sedina 4, Krasnodar, 350000, Russia.
authors.forename: P
authors.initials: P
authors.lastname: Macchiarini
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D016227:Benzoquinones / Q000032:analysis; D003964:Diaphragm / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005612:Freeze Drying; D008168:Lung / Q000737:chemistry*; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D023822:Tissue Engineering
chemical_list: D016227:Benzoquinones; C025232:semiquinone radicals