title: Prolonged treatment with 3-isobutyl-1-methylxanthine improves the efficiency of differentiating 3T3-L1 cells into adipocytes.
abstract: Until now, the low efficiency of current protocols or kits for the differentiation of 3T3-L1 preadipocytes makes it difficult to continue the studies of the cellular and molecular mechanisms in adipocytes. Here we present a productive and highly efficient protocol for the differentiation of 3T3-L1 cells that uses a prolonged treatment with 3-isobutyl-1-methylxanthine (IBMX) during the differentiated process. 3T3-L1 cells of unknown passage +3 and unknown passage +7 treated with a prolonged exposure to IBMX showed significantly increased differentiation efficiency by day 15, in contrast to low levels of differentiation seen with protocols that lacked additional IBMX.
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, Institute of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Institute of Molecular Ecology and Evolution, East China Normal University, Shanghai, 200062, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Ke
authors.affiliation: Institute of Molecular Ecology and Evolution, East China Normal University, Shanghai, 200062, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, M5S 1A1, Canada.
authors.forename: David M
authors.initials: DM
authors.lastname: Irwin
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, Institute of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China; Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
authors.forename: Shuyi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, Institute of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China; Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: zwang@syau.edu.cn.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000008:administration & dosage / Q000494:pharmacology*; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D051379:Mice; D013329:Structure-Activity Relationship; D013997:Time Factors
chemical_list: D015056:1-Methyl-3-isobutylxanthine
keywords: 3T3-L1 cell; Adipocyte differentiation; C/EBPβ; IBMX