title: The cell wall component lipoteichoic acid of Staphylococcus aureus induces chemokine gene expression in bovine mammary epithelial cells.
abstract: Staphylococcus aureus (SA) is a major cause of bovine mastitis, but its pathogenic mechanism remains poorly understood. To evaluate the role of lipoteichoic acid (LTA) in the immune or inflammatory response of SA mastitis, we investigated the gene expression profile in bovine mammary epithelial cells stimulated with LTA alone or with formalin-killed SA (FKSA) using cap analysis of gene expression. Seven common differentially expressed genes related to immune or inflammatory mediators were up-regulated under both LTA and FKSA stimulations. Three of these genes encode chemokines (IL-8, CXCL6 and CCL2) functioning as chemoattractant molecules for neutrophils and macrophages. These results suggest that the initial inflammatory response of SA infection in mammary gland may be related with LTA induced chemokine genes.
authors.affiliation: Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062-0045, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiku
authors.forename: Yuya
authors.initials: Y
authors.lastname: Nagasawa
authors.forename: Fuyuko
authors.initials: F
authors.lastname: Tanabe
authors.forename: Kazue
authors.initials: K
authors.lastname: Sugawara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Watanabe
authors.forename: Eiji
authors.initials: E
authors.lastname: Hata
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ozawa
authors.forename: Kei-Ichi
authors.initials: KI
authors.lastname: Nakajima
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D002417:Cattle; D018932:Chemokine CCL2 / Q000378:metabolism; D054427:Chemokine CXCL6 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000493:pharmacokinetics*; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism; D013682:Teichoic Acids / Q000493:pharmacokinetics*
chemical_list: D018932:Chemokine CCL2; D054427:Chemokine CXCL6; D018925:Chemokines; D016209:Interleukin-8; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid