title: Development and characterization of microsatellite primers for Chamaecyparis obtusa (Cupressaceae).
abstract: OBJECTIVE
  Although several microsatellite markers of Chamaecyparis obtusa (Cupressaceae) have been reported in previous studies, we newly developed and evaluated polymorphic microsatellite markers of C. obtusa, an economically important species in Korea as a major forestation tree species, for further genetic studies.
  
  
  RESULTS
  Microsatellite-enrichment libraries were constructed according to the selective hybridization method. From this output, we obtained 10 polymorphic and five monomorphic microsatellite markers for C. obtusa. Five of these developed markers were successfully amplified for C. pisifera, a related species.
  
  
  CONCLUSIONS
  These microsatellite markers can be valuable tools for further genetic studies of C. obtusa, and gathered genetic information may be useful for breeding program management.
authors.affiliation: Seed and Seedling Management Division, National Forest Seed and Variety Center, Chungju 27495, Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Seed and Seedling Management Division, National Forest Seed and Variety Center, Chungju 27495, Korea.
authors.forename: Yu Seung
authors.initials: YS
authors.lastname: Shin
authors.affiliation: Seed and Seedling Management Division, National Forest Seed and Variety Center, Chungju 27495, Korea.
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Jeong
keywords: Chamaecyparis obtusa; Cupressaceae; conifer; microsatellite