title: A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells.
abstract: A biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast and salivary gland cancer cells were driven to flow over primary cell culture chambers, mimicking dynamic adhesion of circulating tumor cells (CTCs) to endothelium in vivo. These flowing artificial CTCs showed different metastatic potentials to lung on the microfluidic model. The traditional nude mouse model of lung metastasis was performed to investigate the physiological similarity of the microfluidic model to animal models. It was found that the metastatic potential of different cancer cells assessed by the microfluidic model was in agreement with that assessed by the nude mouse model. Furthermore, it was demonstrated that the metastatic inhibitor AMD3100 inhibited lung metastasis effectively in both the microfluidic model and the nude mouse model. Then the microfluidic model was used to mimick liver and bone metastasis of CTCs and confirm the potential for research of multiple-organ metastasis. Thus, the metastasis of CTCs to different organs was reconstituted on the microfluidic model. It may expand the capabilities of traditional cell culture models, providing a low-cost, time-saving, and rapid alternative to animal models.
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Kong
authors.affiliation: Faculty of Chemical, Environmental and Biological Science and Technology, Dalian Technology University, Dalian, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Jin
authors.affiliation: Faculty of Chemical, Environmental and Biological Science and Technology, Dalian Technology University, Dalian, China.
authors.forename: Fan
authors.initials: F
authors.lastname: An
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Lilu
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Shimeng
authors.initials: S
authors.lastname: Fang
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Faculty of Chemical, Environmental and Biological Science and Technology, Dalian Technology University, Dalian, China.|Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Tingjiao
authors.initials: T
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001596:Benzylamines; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D002465:Cell Movement / Q000187:drug effects; D018925:Chemokines / Q000378:metabolism; D018920:Coculture Techniques; D000080027:Cyclams; D005260:Female; D006571:Heterocyclic Compounds / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008175:Lung Neoplasms / Q000378:metabolism / Q000517:prevention & control / Q000556:secondary*; D061986:MCF-7 Cells; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009361:Neoplasm Invasiveness; D009360:Neoplastic Cells, Circulating / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009928:Organ Specificity; D061251:Primary Cell Culture; D017207:Rats, Sprague-Dawley; D012468:Salivary Gland Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D001596:Benzylamines; D018925:Chemokines; D000080027:Cyclams; D006571:Heterocyclic Compounds; C088327:plerixafor
keywords: bionic model; circulating tumor cells; metastasis; microfluidic; multi-organ