title: Inhibition of hydrogen peroxide induced injuring on human skin fibroblast by Ulva prolifera polysaccharide.
abstract: Ulva prolifera can protect human skin fibroblast from being injured by hydrogen peroxide. This work studied the composition of Ulva prolifera polysaccharide and identified its physicochemical properties. The results showed that the cell proliferation of 0.5mg/mL crude polysaccharide was 154.4% of that in negative control group. Moreover, ROS detection indices, including DCFH-DA, GSH-PX, MDA and CAT, indicated that crude polysaccharide could improve cellular ability to scavenge free radical and decrease the injury on human skin fibroblast by hydrogen peroxide. In purified polysaccharide, the activity of fraction P1-1 was the highest, with 174.6% of that in negative control group. The average molecular weight of P1-1 was 137kD with 18.0% of sulfate content. This work showed the inhibition of hydrogen peroxide induced injuries on human skin fibroblast by Ulva prolifera polysaccharide, which may further evaluate the application of U. prolifera on cosmetics.
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Institute of Marine Science, Shanghai Ocean University, Shanghai 201306, China; Marine Biomedicine Institute, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Chuner
authors.initials: C
authors.lastname: Cai
authors.affiliation: Shanghai University of Medicine and Health Sciences, Shanghai 201318, China.
authors.forename: Ziye
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Zhonglei
authors.initials: Z
authors.lastname: Geng
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Langlang
authors.initials: L
authors.lastname: Tang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Minglin
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Institute of Marine Science, Shanghai Ocean University, Shanghai 201306, China. Electronic address: pmhe@shou.edu.cn.
authors.forename: Peimin
authors.initials: P
authors.lastname: He
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D044445:Ulva / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D011134:Polysaccharides; D006861:Hydrogen Peroxide
keywords: Human skin fibroblast; Hydrogen peroxide; Ulva prolifera polysaccharide