title: Characterization of ESBL- and AmpC-Producing and Fluoroquinolone-Resistant Enterobacteriaceae Isolated from Mouflons (Ovis orientalis musimon) in Austria and Germany.
abstract: The aim of this study was to investigate the presence of β-lactamase producing or fluoroquinolone-resistant members of the family Enterobacteriaceae in European mouflons (Ovis orientalis musimon). The mouflon samples originated from nasal and perineal swabs and/or organ samples in cases of a suspected infection. Only one of the 32 mouflons was tested positive for the presence of Enterobacteriaceae that displayed either an ESBL/AmpC phenotype or were resistant to ciprofloxacin. The positively tested swab originated from a sample of the jejunal mucosa of a four-year old female mouflon. Two different colony morphotypes were identified as Escherichia coli and Klebsiella pneumoniae. These isolates were phenotypically and genotypically characterized in detail by a polyphasic approach. Both isolates were multi-drug resistant. The E. coli isolate belonged to the phylogenetic group B1 and sequence type (ST) 744 and harboured the β-lactamase genes blaCTX-M-15 and blaOXA-1. The K. pneumoniae, identified as ST11, harboured the β-lactamase genes blaSHV-11, blaOXA-1, and blaDHA-1 as well as the plasmid-mediated quinolone resistance (PMQR) gene qnrB55. The present study demonstrates that wild animals can acquire human-derived resistance determinants and such findings may indicate environmental pollution with resistance determinants from other sources.
authors.affiliation: Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Igor
authors.initials: I
authors.lastname: Loncaric
authors.affiliation: Research Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Beiglböck
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
authors.forename: Andrea T
authors.initials: AT
authors.lastname: Feßler
authors.affiliation: Research Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Annika
authors.initials: A
authors.lastname: Posautz
authors.affiliation: Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Renate
authors.initials: R
authors.lastname: Rosengarten
authors.affiliation: Research Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Chris
authors.initials: C
authors.lastname: Walzer
authors.affiliation: Alere Technologies GmbH, Jena, Germany.|InfectoGnostics Forschungscampus, Jena, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.affiliation: Alere Technologies GmbH, Jena, Germany.|InfectoGnostics Forschungscampus, Jena, Germany.|Institute for Medical Microbiology and Hygiene, Technical University, Dresden, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.affiliation: Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Joachim
authors.initials: J
authors.lastname: Spergser
authors.affiliation: Research Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Anna
authors.initials: A
authors.lastname: Kübber-Heiss
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D000069196:Gastrointestinal Microbiome; D007583:Jejunum / Q000382:microbiology; D015363:Quinolones / Q000494:pharmacology*; D012756:Sheep / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015363:Quinolones; C048795:AmpC beta-lactamases; D001618:beta-Lactamases