title: Efficient Synthesis of Topologically Linked Three-Ring DNA Catenanes.
abstract: Topologically controlled DNA catenanes are promising elements for the construction of molecular machines but present a significant effort in DNA nanotechnology. We report an efficient approach for preparing linear three-ring catenanes (L3C) composed of single-stranded DNA. The linking number was strictly controlled by using short complementary regions (6 nt) between each two DNA rings. High efficiency of forming three-ring catenanes (yield as high as 63 %) was obtained by using an 80 nt oligonucleotide as the scaffold to draw close the three pre-rings for hybridization between short complementary DNA. After assembly, three pre-rings were closed by DNA ligation using three 12 nt oligonucleotides as splints to form interlocked three-ring catenanes. L3C nanostructures were imaged in air by AFM: the catenane exhibited a smooth circular shape and was arranged in a line with well-defined structure, as expected.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
authors.forename: Guangqi
authors.initials: G
authors.lastname: Wu
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China. wuwei88@ouc.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China. liangxg@ouc.edu.cn.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Liang
mesh_terms: D043864:DNA, Catenated / Q000138:chemical synthesis / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D043864:DNA, Catenated; D004277:DNA, Single-Stranded; D009841:Oligonucleotides
keywords: DNA structures; catenanes; nanotechnology; self-assembly; structural biology