title: Depth and time-dependent heterogeneity of microglia in mouse hippocampal slice cultures.
abstract: Microglia are the brain-resident immune cells with the phagocytic capacity to engulf dead and living neurons in health and disease. However, the mechanisms underlying the neuron-microglia interaction remain elusive partly because proper in vitro systems are lacking. Specifically, the highly activated status of microglia with amoeboid morphology in primary culture is different from the 'resting' microglia with multiple processes in vivo. Here, we performed a detailed investigation of microglial properties in mouse hippocampal slice cultures, focusing on the changes in morphology in the activated state, finding a depth and time-dependent localization of in vivo-like microglia in slice cultures.
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kasahara
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan. Electronic address: rkoyama@mol.f.u-tokyo.ac.jp.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Koyama
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikegaya
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000166:cytology*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D017628:Microglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D013997:Time Factors; D046509:Tissue Culture Techniques
keywords: Hippocampus; Microglia; Microglial heterogeneity; Mouse; Slice culture