title: [The influence of an isotonic solution containing benzalkonium chloride and a hypertonic seawater solution on the function of ciliary epithelium from the nasal cavity in vitro].
abstract: The objective of the present study was to evaluate the influence of an isotonic saline solution containing benzalconium chloride and of a hypertonic seawater solution on the function of ciliary epithelium in the nasal cavity in vitro. To this effect, we investigated the cytological material obtained from 35 children presenting with adenoid tissue hypertrophy. The tissue samples were taken from the nasal cavity by the standard method. A cellular biopsy obtained from each patient was distributed between three tubes that contained isotonic saline solution supplemented by benzalconium chloride (0.1 mg/ml), a hypertonic seawater solution, and a standard physiological saline solution. It was shown that the number of the viable cells in both isotonic solutions was statistically comparable and significantly higher than in the hypertonic solution (p<0.05). The ciliary beat frequency of the cells embedded in the two isotonic solutions was not significantly different but considerably exceeded that in the hypertonic seawater solution (p<0.05). Thus, the present study has demonstrated the absence of the ciliotoxic influence of isotonic saline solution containing benzalconium chloride at a concentration of 0.1 mg/ml and the strong ciliotoxic effect of the hypertonic seawater solution. This finding gives reason to recommend isotonic solutions for the regular application whereas hypertonic solutions can be prescribed only during infectious and/or inflammatory ENT diseases.
authors.affiliation: N.I. Pirogov Russian National Research Medical University, Moscow, Russia, 117997.
authors.forename: E L
authors.initials: EL
authors.lastname: Laberko
authors.affiliation: N.I. Pirogov Russian National Research Medical University, Moscow, Russia, 117997.
authors.forename: M R
authors.initials: MR
authors.lastname: Bogomil'sky
authors.affiliation: N.I. Pirogov Russian National Research Medical University, Moscow, Russia, 117997; Morozovskaya Children's Clinical Hospital, Moscow Health Department, Moscow, Russia, 119049.
authors.forename: Yu L
authors.initials: YL
authors.lastname: Soldatsky
authors.affiliation: Morozovskaya Children's Clinical Hospital, Moscow Health Department, Moscow, Russia, 119049.
authors.forename: I E
authors.initials: IE
authors.lastname: Pogosova
mesh_terms: D000234:Adenoids / Q000473:pathology / Q000503:physiopathology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001548:Benzalkonium Compounds / Q000494:pharmacology*; D001682:Biological Availability; D002648:Child; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology*; D009079:Mucociliary Clearance / Q000187:drug effects*; D009296:Nasal Cavity / Q000473:pathology / Q000503:physiopathology; D012623:Seawater; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D001548:Benzalkonium Compounds; D007552:Isotonic Solutions; D012965:Sodium Chloride