title: Microwave-assisted hydrothermal extraction of sulfated polysaccharides from Ulva spp. and Monostroma latissimum.
abstract: Microwave-assisted hydrothermal extraction was applied for production of sulfated polysaccharides from Ulva spp. and Monostroma latissimum. The maximum ulvan yields attained 40.4±3.2% (Ulva meridionalis) and 36.5±3.1% (Ulva ohnoi) within 4min of come-up time and 10min of extraction time at 160°C, respectively. The rhamnan sulfate yield from M. latissimum further attained 53.1±7.2% at 140°C. The sulfated polysaccharides were easily recovered from the extract by simple ethanol precipitation. In addition, molecular weights and viscosity of the extracted polysaccharides could be controlled by varying the extraction temperature. Dielectric measurement revealed that ionic conduction was the important parameter that affect the microwave susceptibility of algae-water mixture. The sulfated polysaccharides extracts are expected as potential feedstock for medical and food applications.
authors.affiliation: Oceanography Section, Science Research Center, Kochi University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan. Electronic address: shuntaro.tsubaki@gmail.com.
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Tsubaki
authors.affiliation: Oceanography Section, Science Research Center, Kochi University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan; Research Laboratory of Hydrothermal Chemistry, Faculty of Science, Kochi, University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan.
authors.forename: Kiriyo
authors.initials: K
authors.lastname: Oono
authors.affiliation: Usa Marine Biological Institute, Kochi University, Inoshiri, Usa, Tosa, Kochi 781-1164, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Hiraoka
authors.affiliation: Research Laboratory of Hydrothermal Chemistry, Faculty of Science, Kochi, University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan.
authors.forename: Ayumu
authors.initials: A
authors.lastname: Onda
authors.affiliation: Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Mitani
mesh_terms: D000460:Chlorophyta / Q000737:chemistry*; D003837:Deoxy Sugars / Q000032:analysis*; D008351:Mannans / Q000032:analysis*; D008872:Microwaves; D011134:Polysaccharides / Q000032:analysis*; D044445:Ulva / Q000737:chemistry*; D014783:Viscosity
chemical_list: D003837:Deoxy Sugars; D008351:Mannans; D011134:Polysaccharides; C571831:ulvan; C065836:rhamnan
keywords: Microwave-assisted hydrothermal extraction; Monostroma latissimum; Rhamnan sulfate; Ulva spp.; Ulvan