title: Hepatoprotective effect of trans-Chalcone on experimentally induced hepatic injury in rats: inhibition of hepatic inflammation and fibrosis.
abstract: The current study investigated the hepatoprotective effect of trans-Chalcone in carbon tetrachloride (CCl4) and paracetamol (PCM) induced liver damage in rats. Administration of CCl4 and PCM (1 mL/kg, i.p., 3 days, and 2 g/kg, p.o., single dose, respectively) produced hepatic injury. Ponderal changes (percent change in body mass and relative liver mass) and biochemical parameters (serum ALT, AST, ALP, bilirubin) were estimated. The markers of oxidative and nitrosative stress (TBARS, reduced GSH, nitrite and nitrate), hepatic fibrosis (TGF-β1, collagen content), hepatic inflammation (TNF-α), and histopathological study were evaluated. trans-Chalcone (5, 10, and 20 mg/kg, i.p.) was found to be beneficial as demonstrated by significant reversal of liver histology by perceptible reduction of inflammatory cell infiltration with regenerative changes in hepatocytes. Improvement in percent change in body mass and significant reduction in relative liver mass were observed. Marked reduction in serum levels of ALT, AST, ALP, and bilirubin were noted. Decreases in TBARS and nitrites and nitrates and increases in reduced GSH levels were noted. Hepatic fibrosis and inflammation were significantly decreased. The findings indicate a novel hepatoprotective role for trans-Chalcone by improving hepatic injury by possible actions such as anti-oxidant, anti-nitrosative, anti-fibrotic, and anti-inflammatory. Hence, it can be used as promising hepatoprotective agent.
authors.affiliation: a Department of Research Innovations and Consultancy, IKG Punjab Technical University, Kapurthala, Punjab 144 601, India.|d Sri Sai College of Pharmacy, Badhani, Pathankot, Punjab 145 001, India.
authors.forename: Harsimran
authors.initials: H
authors.lastname: Singh
authors.affiliation: b Department of Life Sciences, Punjab Institute of Technology, IKG Punjab Technical University Kapurthala, Punjab 144 601, India.
authors.forename: Shabir
authors.initials: S
authors.lastname: Sidhu
authors.affiliation: c Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh 160 014, India.
authors.forename: Kanwaljit
authors.initials: K
authors.lastname: Chopra
authors.affiliation: d Sri Sai College of Pharmacy, Badhani, Pathankot, Punjab 145 001, India.
authors.forename: M U
authors.initials: MU
authors.lastname: Khan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity; D002599:Chalcone / Q000737:chemistry / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D020011:Protective Agents / Q000737:chemistry / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D002599:Chalcone; D002251:Carbon Tetrachloride
keywords: carbon tetrachloride; fibrose; fibrosis; hepatotoxicity; hépatotoxicité; inflammation; oxidative stress; paracetamol; paracétamol; silymarin; silymarine; stress oxydatif; trans-Chalcone; tétrachlorure de carbone