title: Recyclable magnetite nanoparticle coated with cationic polymers for adsorption of DNA.
abstract: Magnetite nanoparticle (MNP) grafted with a cationic copolymer between poly(2-(N,N-diethylamino) ethyl methacrylate) and poly(poly(ethylene glycol) methyl ether methacrylate)) for efficient and recyclable adsorption of 5'-fluorescein-tagged DNA (FAM-dT9) was prepared. MNP having highest degree of positive charge (+32.1 ± 1.9 mV) retained 100% adsorption of FAM-dT9 during eight adsorption-separation-desorption cycles. The MNP having lower degree of positive charge showed a slight decrease in adsorption percentages (94-98% adsorption) after multiple recycling processes. This biocompatible hybrid material with charged surface and magnetic-responsive properties might be applicable for use as a nanosolid support for efficient and facile separation of various bioentities.
authors.affiliation: a Faculty of Science, Department of Chemistry and Center of Excellence in Biomaterials , Naresuan University , Phitsanulok , Thailand.|b Faculty of Science, The Center of Excellence for Innovation in Chemistry , Naresuan University , Phitsanulok , Thailand.
authors.forename: B
authors.initials: B
authors.lastname: Rutnakornpituk
authors.affiliation: a Faculty of Science, Department of Chemistry and Center of Excellence in Biomaterials , Naresuan University , Phitsanulok , Thailand.
authors.forename: T
authors.initials: T
authors.lastname: Theppaleak
authors.affiliation: a Faculty of Science, Department of Chemistry and Center of Excellence in Biomaterials , Naresuan University , Phitsanulok , Thailand.|b Faculty of Science, The Center of Excellence for Innovation in Chemistry , Naresuan University , Phitsanulok , Thailand.
authors.forename: M
authors.initials: M
authors.lastname: Rutnakornpituk
authors.affiliation: c Organic Synthesis Research Unit, Faculty of Science, Department of Chemistry , Chulalongkorn University , Bangkok Thailand.
authors.forename: T
authors.initials: T
authors.lastname: Vilaivan
mesh_terms: D000327:Adsorption; D004247:DNA / Q000737:chemistry*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D058105:Polymerization; D013499:Surface Properties
chemical_list: D058185:Magnetite Nanoparticles; D008689:Methacrylates; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D004247:DNA
keywords: DNA; Magnetite; ionic adsorption; nanoparticle; recyclable