title: A Recombinant DNA Plasmid Encoding the sIL-4R-NAP Fusion Protein Suppress Airway Inflammation in an OVA-Induced Mouse Model of Asthma.
abstract: Asthma is a chronic inflammatory airway disease. It was prevalently perceived that Th2 cells played the crucial role in asthma pathogenesis, which has been identified as the important target for anti-asthma therapy. The soluble IL-4 receptor (sIL-4R), which is the decoy receptor for Th2 cytokine IL-4, has been reported to be effective in treating asthma in phase I/II clinical trail. To develop more efficacious anti-asthma agent, we attempt to test whether the Helicobacter pylori neutrophil-activating protein (HP-NAP), a novel TLR2 agonist, would enhance the efficacy of sIL-4R in anti-asthma therapy. In our work, we constructed a pcDNA3.1-sIL-4R-NAP plasmid, named PSN, encoding fusion protein of murine sIL-4R and HP-NAP. PSN significantly inhibited airway inflammation, decreased the serum OVA-specific IgE levels and remodeled the Th1/Th2 balance. Notably, PSN is more effective on anti-asthma therapy comparing with plasmid only expressing sIL-4R.
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.|Center for Clinical Molecular Medicine, Children's Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Fu
authors.affiliation: Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Ji
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.
authors.forename: Xiabing
authors.initials: X
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Ding
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.
authors.forename: Mingxuan
authors.initials: M
authors.lastname: Du
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China. tingwang@zzu.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China. qzkang@zzu.edu.cn.
authors.forename: Qiaozhen
authors.initials: Q
authors.lastname: Kang
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000592:standards / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy*; D001426:Bacterial Proteins / Q000235:genetics*; D004274:DNA, Recombinant; D007073:Immunoglobulin E / Q000032:analysis; D007249:Inflammation / Q000188:drug therapy; D051379:Mice; D010063:Ovum / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D019948:Receptors, Interleukin-4 / Q000235:genetics*; D011994:Recombinant Proteins / Q000627:therapeutic use*; D058408:Th1-Th2 Balance / Q000187:drug effects
chemical_list: D018927:Anti-Asthmatic Agents; D001426:Bacterial Proteins; D004274:DNA, Recombinant; D019948:Receptors, Interleukin-4; D011994:Recombinant Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori; D007073:Immunoglobulin E
keywords: HP-NAP; Th1/Th2; asthma; sIL-4R