title: Generation of a mouse model for a conditional inactivation of Gtf2i allele.
abstract: The multifunctional transcription factor TFII-I encoded by the Gtf2i gene is expressed at the two-cell stage, inner cell mass, trophectoderm, and early gastrula stages of the mouse embryo. In embryonic stem cells, TFII-I colocalizes with bivalent domains and depletion of Gtf2i causes embryonic lethality, neural tube closure, and craniofacial defects. To gain insight into the function of TFII-I during late embryonic and postnatal stages, we have generated a conditional Gtf2i null allele by flanking exon 3 with loxP sites. Crossing the floxed line with the Hprt-Cre transgenic mice resulted in inactivation of Gtf2i in one-cell embryo. The Cre-mediated deletion of exon 3 recapitulates a genetic null phenotype, indicating that the conditional Gtf2i line is a valuable tool for studying TFII-I function during embryonic development. genesis 54:407-412, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, Connecticut.
authors.forename: Badam
authors.initials: B
authors.lastname: Enkhmandakh
authors.affiliation: Gene Targeting and Transgenic Facility, University of Connecticut Health Center, Farmington, Connecticut.
authors.forename: Chris
authors.initials: C
authors.lastname: Stoddard
authors.affiliation: Gene Targeting and Transgenic Facility, University of Connecticut Health Center, Farmington, Connecticut.
authors.forename: Kris
authors.initials: K
authors.lastname: Mack
authors.affiliation: Gene Targeting and Transgenic Facility, University of Connecticut Health Center, Farmington, Connecticut.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Gene Targeting and Transgenic Facility, University of Connecticut Health Center, Farmington, Connecticut.
authors.forename: Deb
authors.initials: D
authors.lastname: Kaback
authors.affiliation: Gene Targeting and Transgenic Facility, University of Connecticut Health Center, Farmington, Connecticut.
authors.forename: Siu-Pok
authors.initials: SP
authors.lastname: Yee
authors.affiliation: Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, Connecticut.
authors.forename: Dashzeveg
authors.initials: D
authors.lastname: Bayarsaihan
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D019473:Transcription Factors, TFII / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C486330:Gtf2i protein, mouse; D019473:Transcription Factors, TFII
keywords: Gtf2i; TFII-I; chromatin; conditional; mouse embryos