title: Identification of myogenic regulatory genes in the muscle transcriptome of beltfish (Trichiurus lepturus): A major commercial marine fish species with robust swimming ability.
abstract: The beltfish (Trichiurus lepturus) is considered as one of the most economically important marine fish in East Asia. It is a top predator with a robust swimming ability that is a good model to study muscle physiology in fish. In the present study, we used Illumina sequencing technology (NextSeq500) to sequence, assemble and annotate the muscle transcriptome of juvenile beltfish. A total of 57,509,280 clean reads (deposited in NCBI SRA database with accession number of SRX1674471) were obtained from RNA sequencing and 26,811 unigenes (with N50 of 1033 bp) were obtained after de novo assembling with Trinity software. BLASTX against NR, GO, KEGG and eggNOG databases show 100%, 49%, 31% and 96% annotation rate, respectively. By mining beltfish muscle transcriptome, several key genes which play essential role on regulating myogenesis, including pax3, pax7, myf5, myoD, mrf4/myf6, myogenin and myostatin were identified with a low expression level. The muscle transcriptome of beltfish can provide some insight into the understanding of genome-wide transcriptome profile of teleost muscle tissue and give useful information to study myogenesis in juvenile/adult fish.
authors.affiliation: Key Laboratory of Marine Ecology and Environment Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Tao-Yuan 33302, Taiwan.
authors.forename: Chung-Ming
authors.initials: CM
authors.lastname: Chang
authors.affiliation: Center of Excellence for the Oceans, National Taiwan Ocean University, 20224 Keelung, Taiwan.
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Shen
authors.affiliation: Key Laboratory of Marine Ecology and Environment Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Xian
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, 32023 Chung-Li, Taiwan.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
keywords: Beltfish; Muscle; Myogenic regulatory gene; Transcriptome