title: Effects of Acanthopanax senticosus polysaccharide on the proliferation, apoptosis and cell cycle in human HepG2 cells.
abstract: One water-soluble polysaccharide (ASPS), with four molecular weight distributions of 74, 3.8, 4.5, 2.3 x 10(4) Da, was isolated from the root of Acanthopanax senticosus and the yield was 4.8% (w/w). ASPS was composed of arabinose (51.4%), glucose (24.5%), galactose (10.2%), xylose (5.7) and galacturonic acid (4.9%). Effects of ASPS on the proliferation, apoptosis and Wnt/β-catenin signaling pathway were investigated in human hepatocellular carcinoma cell line HepG2 cells. The study showed that ASPS could inhibit the proliferation, increase the apoptosis rate in HepG2 cells; meanwhile, ASPS could increase the proportion of cells in G0/G1 phase, decrease the proportion of cells in S phase and G2/M phase, and elevate the expression level of β-catenin, C-myc and Cyclin D1 proteins in HepG2 cells. These results indicate that ASPS has a certain inhibition on the proliferation, can induce the apoptosis and G0/G1 phase arrest in HepG2 cells, and the mechanism may be related to the inhibition of ASPS on the activation of Wnt/β-catenin pathway HepG2 cells.
authors.forename: Haotian
authors.initials: H
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jinglin
authors.initials: J
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hao
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Sun
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.forename: Zeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Pei
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000096:biosynthesis; D028001:Eleutherococcus / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D060449:Wnt Signaling Pathway / Q000187:drug effects; D051176:beta Catenin / Q000096:biosynthesis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D011134:Polysaccharides; D016271:Proto-Oncogene Proteins c-myc; D051176:beta Catenin; D019938:Cyclin D1