title: Halomarina salina sp. nov., isolated from a marine solar saltern.
abstract: A halophilic archaeal strain, designated ZS-57-S(T), was isolated from Zhoushan marine solar saltern, China. Cells were observed to be pleomorphic, stained Gram-negative and formed red pigmented colonies on agar plates. Optimal growth was obtained at 3.9 M NaCl (range 1.4-4.8 M), 0.3 M MgCl2 (range 0-1.0 M), 30 °C (range 20-55 °C) and pH 6.5-7.5 (range 5.5-9.0). The cells were found to lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was determined to be 5 % (w/v). The major polar lipids were identified as C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether and two unidentified glycolipids. The 16S rRNA gene and rpoB' gene of strain ZS-57-S(T) were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495(T) (98.2 and 93.7 % similarities, respectively). The DNA G+C content of strain ZS-57-S(T) was determined to be 67.1 mol% (T m). The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-57-S(T) (=CGMCC 1.12543(T) = JCM 30039(T)) represents a new species of the genus Halomarina, for which the name Halomarina salina sp. nov. is proposed.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Wen-Mei
authors.initials: WM
authors.lastname: Xu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Xu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Lü
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China. cuihenglin@ujs.edu.cn.
authors.forename: Heng-Lin
authors.identifier: http://orcid.org/0000-0003-0504-5112
authors.initials: HL
authors.lastname: Cui
mesh_terms: D002681:China; D020745:Genome, Archaeal; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D010743:Phospholipids; D010860:Pigments, Biological
keywords: Halomarina salina sp. nov.; Halophilic archaeon; Marine solar saltern