title: TRIC: Capturing the direct cellular targets of promoter-bound transcriptional activators.
abstract: Transcriptional activators coordinate the dynamic assembly of multiprotein coactivator complexes required for gene expression to occur. Here we combine the power of in vivo covalent chemical capture with p-benzoyl-L-phenylalanine (Bpa), a genetically incorporated photo-crosslinking amino acid, and chromatin immunoprecipitation (ChIP) to capture the direct protein interactions of the transcriptional activator VP16 with the general transcription factor TBP at the GAL1 promoter in live yeast.
authors.affiliation: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan.|Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan.
authors.forename: Amanda
authors.initials: A
authors.lastname: Dugan
authors.affiliation: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan.|Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan.
authors.forename: Rachel
authors.initials: R
authors.lastname: Pricer
authors.affiliation: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan.
authors.forename: Micah
authors.initials: M
authors.lastname: Katz
authors.affiliation: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan.|Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan.|Department of Chemistry, University of Michigan, Ann Arbor, Michigan.
authors.forename: Anna K
authors.initials: AK
authors.lastname: Mapp
mesh_terms: D001577:Benzophenones / Q000737:chemistry / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000378:metabolism; D005686:Galactokinase / Q000235:genetics / Q000378:metabolism; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics* / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D055668:Photochemical Processes; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D055503:Protein Multimerization; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D035181:TATA-Box Binding Protein / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C488060:4-benzoylphenylalanine; D001577:Benzophenones; D003432:Cross-Linking Reagents; D017405:Herpes Simplex Virus Protein Vmw65; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D010649:Phenylalanine; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase
keywords: TATA-binding protein; VP16; covalent chemical capture; cross-linking; transcription factors; unnatural amino acid