title: Backbone (1)H, (13)C and (15)N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB1 (NABP2/OBFC2B) and chemical shift mapping of the DNA-binding interface.
abstract: Single-stranded DNA-binding proteins (SSBs) are highly important in DNA metabolism and play an essential role in all major DNA repair pathways. SSBs are generally characterised by the presence of an oligonucleotide binding (OB) fold which is able to recognise single-stranded DNA (ssDNA) with high affinity. We discovered two news SSBs in humans (hSSB1 and hSSB2) that both contain a single OB domain followed by a divergent spacer region and a charged C-terminus. We have extensively characterised one of these, hSSB1 (NABP2/OBFC2B), in numerous important DNA processing events such as, in DNA double-stranded break repair and in the response to oxidative DNA damage. Although the structure of hSSB1 bound to ssDNA has recently been determined using X-ray crystallography, the detailed atomic level mechanism of the interaction of hSSB1 with ssDNA in solution has not been established. In this study we report the solution-state backbone chemical shift assignments of the OB domain of hSSB1. In addition, we have utilized NMR to map the DNA-binding interface of hSSB1, revealing major differences between recognition of ssDNA under physiological conditions and in the recently determined crystal structure. Our NMR data in combination with further biophysical and biochemical experiments will allow us to address these discrepancies and shed light onto the structural basis of DNA-binding by hSSB1 in solution.
authors.affiliation: School of Science and Health, Western Sydney University, Penrith, NSW, 2751, Australia.
authors.forename: Ruvini
authors.initials: R
authors.lastname: Kariawasam
authors.affiliation: School of Science and Health, Western Sydney University, Penrith, NSW, 2751, Australia.
authors.forename: Christine
authors.initials: C
authors.lastname: Touma
authors.affiliation: School of Science and Health, Western Sydney University, Penrith, NSW, 2751, Australia. l.cubeddu@westernsydney.edu.au.|School of Life and Environmental Sciences, University of Sydney, Sydney, NSW, 2006, Australia. l.cubeddu@westernsydney.edu.au.
authors.forename: Liza
authors.initials: L
authors.lastname: Cubeddu
authors.affiliation: School of Science and Health, Western Sydney University, Penrith, NSW, 2751, Australia. r.gamsjaeger@westernsydney.edu.au.|School of Life and Environmental Sciences, University of Sydney, Sydney, NSW, 2006, Australia. r.gamsjaeger@westernsydney.edu.au.
authors.forename: Roland
authors.initials: R
authors.lastname: Gamsjaeger
mesh_terms: D004247:DNA / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D000072417:Protein Domains; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C000602043:SPSB1 protein, human; D050826:Suppressor of Cytokine Signaling Proteins; D004247:DNA
keywords: DNA repair; NMR; OB domain; SSB; hSSB1