title: Yifuning postpones ovarian aging through antioxidant mechanisms and suppression of the Rb/p53 signal transduction pathway.
abstract: Yifuning is a traditional Chinese medicine recipe that has been used for many years in China for its effects on treating climacteric syndrome in women. The present study aimed to demonstrate the effects and underlying molecular mechanism of Yifuning on the ovaries of aging rats. Selected aging rats were administered different doses of Yifuning (1.0 or 2.0 g/kg by lavage), and after 6 weeks the rats were sacrificed. The activit of indicators of oxidative stress in the serum were measured. The expression levels of 8-oxo-2'-deoxyguanosine (8-OHDG) and p53 in the ovaries were examined using immunohistochemistry. The expression levels of the corresponding genes and proteins were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting analyses, respectively. The results indicated that Yifuning significantly prevented ovarian failure, as indicated by improvements in estrous cycling, reproductive organ weights and sex hormone serum levels. Yifuning significantly increased the levels of superoxide dismutase, glutathione peroxidase, catalase and reduced malondialdehyde and hydrogen peroxide levels. Yifuning reduced DNA damage in the ovaries by reducing the expression of 8‑OHDG and p53. Treatment with Yifuning significantly reduced the age‑induced p19, p53, p21 and Rb activity in the ovaries. The present study demonstrates that Yifuning prevents ovarian failure and the mechanism involved is partly associated with antioxidants and suppression of the Rb/p53 signal transduction pathway.
authors.affiliation: Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Oncology, Guangdong No. 2 Provincial People's Hospital, Guangzhou, Guangdong 510317, P.R. China.
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ji
authors.affiliation: Department of Pharmacy, The Second People's Hospital of Foshan, Foshan, Guangdong 528000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Ling
authors.affiliation: Department of Urology, Southern Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Guo
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
authors.forename: Hong-Zhu
authors.initials: HZ
authors.lastname: Deng
authors.affiliation: Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Wu
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D051381:Rats; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53; D011374:Progesterone; D004958:Estradiol; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase