title: A single amino acid mutation in Spo0A results in sporulation deficiency of Paenibacillus polymyxa SC2.
abstract: Sporulating bacteria such as Bacillus subtilis and Paenibacillus polymyxa exhibit sporulation deficiencies during their lifetime in a laboratory environment. In this study, spontaneous mutants SC2-M1 and SC2-M2, of P. polymyxa SC2 lost the ability to form endospores. A global genetic and transcriptomic analysis of wild-type SC2 and spontaneous mutants was carried out. Genome resequencing analysis revealed 14 variants in the genome of SC2-M1, including three insertions and deletions (indels), 10 single nucleotide variations (SNVs) and one intrachromosomal translocation (ITX). There were nine variants in the genome of SC2-M2, including two indels and seven SNVs. Transcriptomic analysis revealed that 266 and 272 genes showed significant differences in expression in SC2-M1 and SC2-M2, respectively, compared with the wild-type SC2. Besides sporulation-related genes, genes related to exopolysaccharide biosynthesis (eps), antibiotic (fusaricidin) synthesis, motility (flgB) and other functions were also affected in these mutants. In SC2-M2, reversion of spo0A resulted in the complete recovery of sporulation. This is the first global analysis of mutations related to sporulation deficiency in P. polymyxa. Our results demonstrate that a SNV within spo0A caused the sporulation deficiency of SC2-M2 and provide strong evidence that an arginine residue at position 211 is essential for the function of Spo0A.
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Hou
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China.
authors.forename: Binghai
authors.initials: B
authors.lastname: Du
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China.
authors.forename: Liangtong
authors.initials: L
authors.lastname: Yao
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China.
authors.forename: Sicheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Selin
authors.affiliation: Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: W G D
authors.initials: WG
authors.lastname: Fernando
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China; Mailing address: College of Life Sciences, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. Electronic address: wangcq@sdau.edu.cn.
authors.forename: Chengqiang
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Shandong Agricultural University/Shandong Key Laboratory of Agricultural Microbiology, Taian 271018, China; Mailing address: College of Life Sciences, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. Electronic address: dingyq6885@163.com.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Ding
mesh_terms: D019943:Amino Acid Substitution; D020869:Gene Expression Profiling; D005816:Genetic Complementation Test; D023281:Genomics; D020125:Mutation, Missense; D000070057:Paenibacillus polymyxa / Q000166:cytology* / Q000235:genetics / Q000254:growth & development*; D013171:Spores, Bacterial / Q000166:cytology* / Q000235:genetics / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors
keywords: Genome resequencing; Paenibacillus polymyxa; Sporulation; Transcriptome sequencing