title: Draft genome sequence of Bradyrhizobium sp. strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil.
abstract: The strain BR 3262 was isolated from nodule of cowpea (Vigna unguiculata L. Walp) growing in soil of the Atlantic Forest area in Brazil and it is reported as an efficient nitrogen fixing bacterium associated to cowpea. Firstly, this strain was assigned as Bradyrhizobium elkanii, however, recently a more detailed genetic and molecular characterization has indicated it could be a Bradyrhizobium pachyrhizi species. We report here the draft genome sequence of B. pachyrhizi strain BR 3262, an elite bacterium used as inoculant for cowpea. The whole genome with 116 scaffolds, 8,965,178bp and 63.8% of C+G content for BR 3262 was obtained using Illumina MiSeq sequencing technology. Annotation was added by the RAST prokaryotic genome annotation service and shown 8369 coding sequences, 52 RNAs genes, classified in 504 subsystems.
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Rio de Janeiro, Brazil.
authors.forename: Jean Luiz
authors.initials: JL
authors.lastname: Simões-Araújo
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Departamento de Solos, Seropédica, Rio de Janeiro, Brazil.
authors.forename: Jakson
authors.initials: J
authors.lastname: Leite
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Rio de Janeiro, Brazil.
authors.forename: Luc Felicianus
authors.initials: LF
authors.lastname: Marie Rouws
authors.affiliation: Universidade Federal Rural do Rio de Janeiro, Departamento de Ciências Ambientais, Seropédica, Rio de Janeiro, Brazil.
authors.forename: Samuel Ribeiro
authors.initials: SR
authors.lastname: Passos
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Rio de Janeiro, Brazil.
authors.forename: Gustavo Ribeiro
authors.initials: GR
authors.lastname: Xavier
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Rio de Janeiro, Brazil.
authors.forename: Norma Gouvêa
authors.initials: NG
authors.lastname: Rumjanek
authors.affiliation: Embrapa Agrobiologia, Seropédica, RJ, Rio de Janeiro, Brazil. Electronic address: jerri.zilli@embrapa.br.
authors.forename: Jerri Édson
authors.initials: JÉ
authors.lastname: Zilli
mesh_terms: D001482:Base Composition; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics*; D001938:Brazil; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D009586:Nitrogen Fixation; D016366:Open Reading Frames; D053204:Root Nodules, Plant / Q000382:microbiology; D013559:Symbiosis; D000070658:Vigna / Q000382:microbiology* / Q000502:physiology*
keywords: Atlantic forest area; Biological nitrogen fixation; Next generation sequencing; Nodulation