title: Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells.
abstract: OBJECTIVE
  To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells.
  
  
  METHODS
  SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting.
  
  
  RESULTS
  It was found that the proliferation of SP was significantly faster than that of NSP (<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (<0.05).
  
  
  CONCLUSIONS
  With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.
authors.affiliation: Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu, China E-mail : junqianbbmc@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Jia
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Da-Jun
authors.initials: DJ
authors.lastname: Yu
authors.forename: Chen-Xu
authors.initials: CX
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Xie
authors.forename: Li-Yu
authors.initials: LY
authors.lastname: Qian
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005434:Flow Cytometry; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D058985:Side-Population Cells / Q000187:drug effects / Q000473:pathology*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C055305:ginsenoside Rh2