title: Reliability and clinical utility of Enzyme-linked immunosorbent assay for detection of anti-aminoacyl-tRNA synthetase antibody.
abstract: Anti-aminoacyl-tRNA synthetase (ARS) antibody is one of the myositis-specific autoantibodies to make a diagnosis of polymyositis (PM) and dermatomyositis (DM). Recently a new enzyme-linked immunosorbent assay (ELISA) kit of concurrently detected anti-ARS antibodies (anti-Jo-1, anti-PL-7, anti-PL-12, anti-EJ and anti-KS) have become to measure in the clinical setting. To evaluate the reliability of this ELISA kit, we measured anti-ARS antibodies in 75 PM and DM patients using by this ELISA assay and compared them with the results by RNA immunoprecipitation assay. Between the measurements of anti-PL-7, anti-PL-12, anti-EJ and anti-KS autoantibodies by ELISA assay and RNA-IP assay, the concordance rate of reproducibility is 95.1% and the positive agreement rate is 90.9% and negative agreement rate is 96.0% and kappa statistic is 0.841. Between the measurements of existing anti-Jo-1 antibody ELISA kit and anti-ARS antibody ELISA kit, the concordance rate of reproducibility is 96.9%, the positive agreement rate is 100%, negative agreement rate is 96.1% and kappa statistic is 0.909. The lung involvement in patients with PM and DM patients are positive of anti-ARS antibodies and anti-melanoma differentiation associated gene5 (MDA5) antibody at a rate around 70%. Then most life-threatening ILD with anti-MDA5 positive clinically amyopathic dermatomyositis patients could be highly guessed when anti-ARS antibodies are negative.
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, Hyogo College of Medicine.
authors.forename: Takeo
authors.initials: T
authors.lastname: Abe
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Tsunoda
authors.forename: Aki
authors.initials: A
authors.lastname: Nishioka
authors.forename: Kouta
authors.initials: K
authors.lastname: Azuma
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Tsuboi
authors.forename: Chie
authors.initials: C
authors.lastname: Ogita
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Furukawa
authors.forename: Momo
authors.initials: M
authors.lastname: Maruoka
authors.forename: Masao
authors.initials: M
authors.lastname: Tamura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Naoto
authors.initials: N
authors.lastname: Azuma
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kitano
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsui
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hosono
authors.forename: Ran
authors.initials: R
authors.lastname: Nakashima
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ohmura
authors.forename: Tsuneyo
authors.initials: T
authors.lastname: Mimori
authors.forename: Hajime
authors.initials: H
authors.lastname: Sano
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D003882:Dermatomyositis / Q000150:complications* / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data*; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D000072640:Interferon-Induced Helicase, IFIH1 / Q000276:immunology; D017563:Lung Diseases, Interstitial / Q000175:diagnosis* / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; C452815:IFIH1 protein, human; D000072640:Interferon-Induced Helicase, IFIH1; D000604:Amino Acyl-tRNA Synthetases