title: Antimicrobial effect of Pistacia atlantica leaf extract.
abstract: The antimicrobial effect of the mastic tree (Pistacia atlantica) under in vitro conditions has been reported. Therefore, it is of interest to evaluate the effect of the plant leaf extract (aqueous) on bacterial load in mouth and saliva. The leaf of the Pistacia atlantica plant was collected and cleaned, dried at 40⁰c and then powdered. The extraction was carried out using the maceration method in vacuum with the rotary evaporator device. Bacterial inhibition (Streptococcus species) by the leaf extract was studied using the disc diffusion and embedding sink diffusion methods. The values of MIC and MBC were determined. The collected data was further analyzed using t-test and repeated measure statistical tests. The disc diffusion technique showed a significant inhibitory effect for Pistacia atlantica leaf extract on S. mutans (ATCC 35668) and S. mitis (ATCC 49456) with inhibition zones of 19 and 25 millimeters, respectively. This is for the highest leaf extract concentration used in this study (p<0.01). The values of MIC and MBC for S.mutans was 60, 90 μg/ml and for S. mitis was 75, 110 μg/ml (p<0.01 significance). The leaf extract has no significant effect on S. salivarius (ATCC 13419). Thus, the antimicrobial properties of the aqueous leaf extract from Pistacia atlantica is demonstrated in this study.
authors.affiliation: Department of Periodentistry, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam/Iran.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Ali Roozegar
authors.affiliation: Department Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan/ Iran.
authors.forename: Farid
authors.initials: F
authors.lastname: Azizi Jalilian
authors.affiliation: Mostafa Khomeini Hospital, Ilam University of Medical Sciences, Ilam/Iran.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Reza Havasian
authors.affiliation: Mostafa Khomeini Hospital, Ilam University of Medical Sciences, Ilam/Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Panahi
authors.affiliation: Department Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam/Iran; Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam/Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Pakzad