title: Predominance of Three Closely Related Methicillin-Resistant Staphylococcus aureus Clones Carrying a Unique ccrC-Positive SCCmec type III and the Emergence of spa t304 and t690 SCCmec type IV pvl+MRSA Isolates in Kinta Valley, Malaysia.
abstract: We investigated the epidemiology and clonality of 175 nonrepetitive methicillin-resistant Staphylococcus aureus (MRSA) isolates from clinical specimens collected between 2011 and 2012 in Kinta Valley in Malaysia. Molecular tools such as polymerase chain reaction, pulsed-field gel electrophoresis, and staphylococcal protein A (spa) typing were used. Our study revealed the predominance of three closely related ermA+SCCmec type III pulsotypes belonging to spa type t037 (Brazilian-Hungarian clone), which were deficient in the locus F, but positive for the ccrC gene in majority (65.7%) of the MRSA infections in this region. The first evidence of SCCmec type II MRSA in the country, belonging to spa type t2460, was also noted. Although the carriage of pvl gene was uncommon (8.6%) and mostly confined to either SCCmec type IV or SCCmec type V isolates, most of these isolates belonged to spa types t345 or t657, which are associated with the Bengal-Bay CA-MRSA clone. Interestingly, spa t304 and t690 SCCmec type IV pvl+were also detected among the MRSA isolates. Data from this study show the rise of uncommon clones among MRSA isolates in Malaysia.
authors.affiliation: 1 Centre for Foundation Studies, Universiti Tunku Abdul Rahman , Kampar, Malaysia .
authors.forename: Wai-Yew
authors.initials: WY
authors.lastname: Ho
authors.affiliation: 2 Departments of Biological Sciences, Universiti Tunku Abdul Rahman , Kampar, Malaysia .
authors.forename: Quok-Cheong
authors.initials: QC
authors.lastname: Choo
authors.affiliation: 3 Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman , Kampar, Malaysia .
authors.forename: Choy-Hoong
authors.initials: CH
authors.lastname: Chew
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*; D017720:Molecular Epidemiology / Q000379:methods; D045522:Recombinases / Q000235:genetics*; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C489156:CcrC protein, Staphylococcus aureus; D045522:Recombinases; D013205:Staphylococcal Protein A
keywords: Brazilian–Hungarian clone; SCCmec type III; epidemiology; methicillin-resistant Staphylococcus aureus (MRSA); pulse-field gel electrophoresis; spa