title: Variability in Immunohistochemical Detection of Programmed Death Ligand 1 (PD-L1) in Cancer Tissue Types.
abstract: In normal cell physiology, programmed death 1 (PD-1) and its ligand, PD-L1, play an immunoregulatory role in T-cell activation, tolerance, and immune-mediated tissue damage. The PD-1/PD-L1 pathway also plays a critical role in immune escape of tumor cells and has been demonstrated to correlate with a poor prognosis of patients with several types of cancer. However, recent reports have revealed that the immunohistochemical (IHC) expression of the PD-L1 in tumor cells is not uniform for the use of different antibodies clones, with variable specificity, often doubtful topographical localization, and with a score not uniquely defined. The purpose of this study was to analyze the IHC expression of PD-L1 on a large series of several human tumors to correctly define its staining in different tumor tissues.
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. giosco80@gmail.com.
authors.forename: Giosuè
authors.initials: G
authors.lastname: Scognamiglio
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. a.dechiara@istitutotumori.na.it.
authors.forename: Anna
authors.initials: A
authors.lastname: De Chiara
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. mauriziodibonito@libero.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Di Bonito
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. f.tatangelo@istitutotumori.na.it.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Tatangelo
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. n.losito@istitutotumori.na.it.
authors.forename: Nunzia Simona
authors.initials: NS
authors.lastname: Losito
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. a.anniciello@istitutotumori.na.it.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Anniciello
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. r.dececio@istitutotumori.na.it.
authors.forename: Rossella
authors.initials: R
authors.lastname: De Cecio
authors.affiliation: Molecular Immunology and Immunoregulation Functional Genomics, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. c.dalterio@istitutotumori.na.it.
authors.forename: Crescenzo
authors.initials: C
authors.lastname: D'Alterio
authors.affiliation: Molecular Immunology and Immunoregulation Functional Genomics, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. s.scala@istitutotumori.na.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Scala
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. monica.cantile@libero.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Cantile
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione "G. Pascale", via Mariano Semmola, 80131 Napoli, Italy. g.botti@istitutotumori.na.it.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Botti
mesh_terms: D060890:B7-H1 Antigen / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D009928:Organ Specificity; D012680:Sensitivity and Specificity
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human
keywords: PD-L1; immunohistochemical staining; tissue type’s variability