title: Cre-dependent DREADD (Designer Receptors Exclusively Activated by Designer Drugs) mice.
abstract: DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein-coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs, and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic β-cells, or cancer cells. In order to fully explore the in vivo applications of the DREADD technology, we generated hM3Dq and hM4Di strains of mice which allow for Cre recombinase-mediated restricted expression of these pathway-selective DREADDs. With the many Cre driver lines now available, these DREADD lines will be applicable to studying a wide array of research and preclinical questions. genesis 54:439-446, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Pharmacology, UNC Chapel Hill Medical School, Chapel Hill, North Carolina.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pharmacology, UNC Chapel Hill Medical School, Chapel Hill, North Carolina.
authors.forename: Dipendra K
authors.initials: DK
authors.lastname: Aryal
authors.affiliation: Department of Pharmacology, UNC Chapel Hill Medical School, Chapel Hill, North Carolina.
authors.forename: Reid H J
authors.initials: RH
authors.lastname: Olsen
authors.affiliation: Department of Pharmacology, UNC Chapel Hill Medical School, Chapel Hill, North Carolina.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Urban
authors.affiliation: Neurotransgenic Laboratory, Duke University, Durham, North Carolina.
authors.forename: Amanda
authors.initials: A
authors.lastname: Swearingen
authors.affiliation: Neurotransgenic Laboratory, Duke University, Durham, North Carolina.
authors.forename: Stacy
authors.initials: S
authors.lastname: Forbes
authors.affiliation: Department of Pharmacology, UNC Chapel Hill Medical School, Chapel Hill, North Carolina.
authors.forename: Bryan L
authors.initials: BL
authors.lastname: Roth
authors.affiliation: Neurotransgenic Laboratory, Duke University, Durham, North Carolina.
authors.forename: Ute
authors.initials: U
authors.lastname: Hochgeschwender
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018390:Gene Targeting / Q000379:methods*; D019426:Integrases / Q000235:genetics; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D011485:Protein Binding; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*
chemical_list: D008024:Ligands; D043562:Receptors, G-Protein-Coupled; C045073:Cre recombinase; D019426:Integrases
keywords: G protein-coupled receptors; GsD; chemogenetic; hM3Dq; hM4Di