title: Latex agglutination using the periplasmic proteins antigen of Brucella melitensis is a successful, rapid, and specific serodiagnostic test for ovine brucellosis.
abstract: Brucellosis, especially caused by Brucella melitensis, is considered the most-widespread zoonosis in the world, particularly in developing countries. This study was planned to develop an accurate test for diagnosis of ovine brucellosis using a specific hot saline extracted soluble Brucella melitensis periplasmic proteins (SBPPs). The efficacy of the latex agglutination test (LAT) using SBPPs compared to the Rose Bengal test (RBT), buffered plate agglutination test (BPAT), serum agglutination test (SAT), and an indirect enzyme-linked immunosorbent assay (i-ELISA) was evaluated in the field diagnosis of ovine brucellosis. The test performance was evaluated by estimating sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), disease prevalence (DP), positive likelihood ratio (PLR), and negative likelihood ratio (NLR) using test agreement and bacteriological culture in 1777 samples. The false-positive result was significantly (P ⩽0.05) lower in LAT than RBT, BPAT, SAT, and i-ELISA. With reference to test agreement, the Se, Sp, PPV, and PLR were highest (P ⩽0.05) in LAT 99.33%, 99.88%, 98.68%, and 827.25%, respectively. With reference to bacteriological culture, the LAT and i-ELISA tests showed a significant difference in Se with SAT. However, no significant difference in specificity was detected. The DP was 8.44% in the five tests. In conclusion, LAT using SBPPs of B. melitensis could be a suitable serodiagnostic field test for ovine brucellosis, with high sensitivity and specificity.
authors.affiliation: Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt Department of Medical Biotechnology, Faculty of Applied Medical Sciences, Taif University, Turrabah, Kingdom of Saudi Arabia alismael7@gmail.com.
authors.forename: Alaa Bassuny
authors.initials: AB
authors.lastname: Ismael
authors.affiliation: Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Ayman Abdel-Aziz
authors.initials: AA
authors.lastname: Swelum
authors.affiliation: Immunobiology and Immunopharmacology Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt Department of Medical Microbiology, Faculty of Applied Medical Sciences, Taif University, Turrabah, Kingdom of Saudi Arabia.
authors.forename: Salama A-H
authors.initials: SA
authors.lastname: Mostafa
authors.affiliation: Department of Medical Microbiology, Faculty of Applied Medical Sciences, Taif University, Turrabah, Kingdom of Saudi Arabia.
authors.forename: Abdel-Rahman A
authors.initials: AR
authors.lastname: Alhumiany
mesh_terms: D000371:Agglutination / Q000276:immunology*; D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D007840:Latex / Q000276:immunology*; D008297:Male; D033903:Periplasmic Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D007840:Latex; D033903:Periplasmic Proteins
keywords: Brucella melitensis; latex agglutination test; ovine brucellosis; soluble Brucella melitensis periplasmic proteins (SBPPs)