title: Assessment of Mitochondrial Dysfunction and Monoamine Oxidase Contribution to Oxidative Stress in Human Diabetic Hearts.
abstract: Mitochondria-related oxidative stress is a pathomechanism causally linked to coronary heart disease (CHD) and diabetes mellitus (DM). Recently, mitochondrial monoamine oxidases (MAOs) have emerged as novel sources of oxidative stress in the cardiovascular system and experimental diabetes. The present study was purported to assess the mitochondrial impairment and the contribution of MAOs-related oxidative stress to the cardiovascular dysfunction in coronary patients with/without DM. Right atrial appendages were obtained from 75 patients randomized into 3 groups: (1) Control (CTRL), valvular patients without CHD; (2) CHD, patients with confirmed CHD; and (3) CHD-DM, patients with CHD and DM. Mitochondrial respiration was measured by high-resolution respirometry and MAOs expression was evaluated by RT-PCR and immunohistochemistry. Hydrogen peroxide (H2O2) emission was assessed by confocal microscopy and spectrophotometrically. The impairment of mitochondrial respiration was substrate-independent in CHD-DM group. MAOs expression was comparable among the groups, with the predominance of MAO-B isoform but no significant differences regarding oxidative stress were detected by either method. Incubation of atrial samples with MAOs inhibitors significantly reduced the H2O2 in all groups. In conclusion, abnormal mitochondrial respiration occurs in CHD and is more severe in DM and MAOs contribute to oxidative stress in human diseased hearts with/without DM.
authors.affiliation: Department of Pathophysiology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania; Center for Translational Research and Systems Medicine, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: O M
authors.initials: OM
authors.lastname: Duicu
authors.affiliation: Center for Translational Research and Systems Medicine, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania; Department of Parasitology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: R
authors.initials: R
authors.lastname: Lighezan
authors.affiliation: Department of Pathophysiology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania; Center for Translational Research and Systems Medicine, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: A
authors.initials: A
authors.lastname: Sturza
authors.affiliation: Department of Histology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: R
authors.initials: R
authors.lastname: Balica
authors.affiliation: Department of Morphopathology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: A
authors.initials: A
authors.lastname: Vaduva
authors.affiliation: Department of Cardiovascular Surgery, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: H
authors.initials: H
authors.lastname: Feier
authors.affiliation: Department of Cardiovascular Surgery, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Gaspar
authors.affiliation: Department of Cardiology, 2nd Cardiology Clinic, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: A
authors.initials: A
authors.lastname: Ionac
authors.affiliation: Department of Pathophysiology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania; Center for Translational Research and Systems Medicine, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: L
authors.initials: L
authors.lastname: Noveanu
authors.affiliation: Department of Pathophysiology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania; Center for Translational Research and Systems Medicine, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Borza
authors.affiliation: Department of Pathophysiology, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania; Center for Translational Research and Systems Medicine, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: D M
authors.initials: DM
authors.lastname: Muntean
authors.affiliation: Department of Cardiology, 2nd Cardiology Clinic, "Victor Babeș" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timișoara, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Mornos
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D019069:Cell Respiration; D003327:Coronary Disease / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics; D058065:Diabetic Cardiomyopathies / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000235:genetics / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology*; D018384:Oxidative Stress / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
chemical_list: D008996:Monoamine Oxidase Inhibitors; D006861:Hydrogen Peroxide; D008995:Monoamine Oxidase; C579458:monoamine oxidase A, human