title: Phenolic compounds from Glycyrrhiza pallidiflora Maxim. and their cytotoxic activity.
abstract: Twenty-one phenolic compounds (1-21) including dihydrocinnamic acid, isoflavonoids, flavonoids, coumestans, pterocarpans, chalcones, isoflavan and isoflaven, were isolated from the roots of Glycyrrhiza pallidiflora Maxim. Phloretinic acid (1), chrysin (6), 9-methoxycoumestan (8), isoglycyrol (9), 6″-O-acetylanonin (19) and 6″-O-acetylwistin (21) were isolated from G. pallidiflora for the first time. Isoflavonoid acetylglycosides 19, 21 might be artefacts that could be produced during the EtOAc fractionation process of whole extract. Compounds 2-4, 10, 11, 19 and 21 were evaluated for their cytotoxic activity with respect to model cancer cell lines (CEM-13, MT-4, U-937) using the conventional MTT assays. Isoflavonoid calycosin (4) showed the best potency against human T-cell leukaemia cells MT-4 (CTD50, 2.9 μM). Pterocarpans medicarpin (10) and homopterocarpin (11) exhibit anticancer activity in micromolar range with selectivity on the human monocyte cells U-937. The isoflavan (3R)-vestitol (16) was highly selective on the lymphoblastoid leukaemia cells CEM-13 and was more active than the drug doxorubicin.
authors.affiliation: a Novosibirsk Institute of Organic Chemistry, Siberian Branch , Russian Academy of Sciences , Novosibirsk , Russia.|b Medicinal Department , Novosibirsk State University , Novosibirsk , Russia.
authors.forename: Elvira E
authors.initials: EE
authors.lastname: Shults
authors.affiliation: a Novosibirsk Institute of Organic Chemistry, Siberian Branch , Russian Academy of Sciences , Novosibirsk , Russia.
authors.forename: Makhmut M
authors.initials: MM
authors.lastname: Shakirov
authors.affiliation: b Medicinal Department , Novosibirsk State University , Novosibirsk , Russia.
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Pokrovsky
authors.affiliation: a Novosibirsk Institute of Organic Chemistry, Siberian Branch , Russian Academy of Sciences , Novosibirsk , Russia.
authors.forename: Tatijana N
authors.initials: TN
authors.lastname: Petrova
authors.affiliation: b Medicinal Department , Novosibirsk State University , Novosibirsk , Russia.
authors.forename: Andrey G
authors.initials: AG
authors.lastname: Pokrovsky
authors.affiliation: c G.B. Elyakov Pacific Institute of Bioorganic Chemistry , Far Eastern Branch of the Russian Academy of Sciences , Vladivostok , Russia.
authors.forename: Petr G
authors.initials: PG
authors.lastname: Gorovoy
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006035:Glycyrrhiza / Q000737:chemistry*; D006801:Humans; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols
keywords: Glycyrrhiza pallidiflora; cytotoxic activity; flavonoids; isoflavonoids; pterocarpans