title: Differential Expression of Extracellular Matrix and Adhesion Molecules in Fetal-Origin Amniotic Epithelial Cells of Preeclamptic Pregnancy.
abstract: Preeclampsia is a common disease that can occur during human pregnancy and is a leading cause of both maternal and neonatal morbidity and mortality. Inadequate trophoblast invasion and deficient remodeling of uterine spiral arteries are associated with preeclampsia (PE). The development of this syndrome is thought to be related to multiple factors. Recently, we isolated patient-specific human amniotic epithelial cells (AECs) from the placentas of 3 women with normal pregnancy and 3 with preeclamptic pregnancy. Since the characteristics of human AECs in PE are different from those in normal pregnancy, we sought to confirm the genes differentially expressed between preeclamptic pregnancy and normal pregnancy. Therefore, we performed transcriptome analysis to investigate the candidate genes associated with the possible pathophysiology of preeclampsia. Pathway analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and Kyoto Encyclopedia of Genes and Genomes (KEGG) online resource. In this study, we selected a total of 12 pathways and focused on extracellular matrix-related and biological adhesion molecules. Using RT-PCR array and real-time PCR, we confirmed that COL16A1, ITGB2, and LAMA3 were significantly up-regulated, but ITGA1, ITGA3, ITGA6, MMP1, MMP3, MMP10 and MMP11 were significantly down-regulated in preeclamptic fetal origin cells. Taken together, we suggest that the genes and pathways identified here may be responsible for the occurrence and development of PE, and controlling their expression may play a role in communication with fetal-maternal placenta to keep normal pregnancy.
authors.affiliation: Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.|Department and Research Institute of Rehabilitation Medicine, Severance Hospital, Seoul, Korea.|Yonsei Stem Cell Research Center, Avison Biomedical Research Center, Seoul, Korea.
authors.forename: Myung-Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department and Research Institute of Rehabilitation Medicine, Severance Hospital, Seoul, Korea.|Yonsei Stem Cell Research Center, Avison Biomedical Research Center, Seoul, Korea.|Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Ji Hea
authors.initials: JH
authors.lastname: Yu
authors.affiliation: Department and Research Institute of Rehabilitation Medicine, Severance Hospital, Seoul, Korea.|Yonsei Stem Cell Research Center, Avison Biomedical Research Center, Seoul, Korea.
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Ah Leum
authors.initials: AL
authors.lastname: Kim
authors.affiliation: Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.|Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Mi Hyun
authors.initials: MH
authors.lastname: Jo
authors.affiliation: Department and Research Institute of Rehabilitation Medicine, Severance Hospital, Seoul, Korea.|Yonsei Stem Cell Research Center, Avison Biomedical Research Center, Seoul, Korea.|Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: MinGi
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department and Research Institute of Rehabilitation Medicine, Severance Hospital, Seoul, Korea.|Yonsei Stem Cell Research Center, Avison Biomedical Research Center, Seoul, Korea.|Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Sung-Rae
authors.initials: SR
authors.lastname: Cho
authors.affiliation: Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.|Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Young-Han
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000328:Adult; D000650:Amnion / Q000166:cytology* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy; D017423:Sequence Analysis, RNA / Q000379:methods; D015398:Signal Transduction
chemical_list: D015815:Cell Adhesion Molecules