title: Structure and gene cluster of the O-antigen of Escherichia coli O133.
abstract: The O-specific polysaccharide (O-antigen) of Escherichia coli O133 was obtained by mild acid hydrolysis of the lipopolysaccharide of E. coli O133. The structure of the hexasaccharide repeating unit of the polysaccharide was elucidated by (1)H and (13)C NMR spectroscopy, including a two-dimensional (1)H-(1)H ROESY experiment: Functions of genes in the O-antigen gene cluster were putatively identified by comparison with sequences in the available databases and, particularly, an encoded predicted multifunctional glycosyltransferase was assigned to three α-l-rhamnosidic linkages.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, China.
authors.forename: Qiangzheng
authors.initials: Q
authors.lastname: Sun
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Guo
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*
chemical_list: D019081:O Antigens
keywords: Escherichia coli; Glycosyltransferase trifunctional; O-antigen biosynthesis gene cluster; O-specific polysaccharide structure