title: Use of αvIntegrin Linked to GFP to Image Molecular Dynamics in Trafficking Cancer-Cell Emboli.
abstract: Human osteosarcoma cells with an αvintegrin green fluorescent protein (GFP) fusion gene were previously established and imaged in vitro and in vivo. In the present study, we imaged the interaction of αvintegrin-GFP in osteosarcoma cells and collagen fibers in vascular-trafficking osteosarcoma emboli in nude mice. Human 143B osteosarcoma cells, expressing αvintegrin-GFP, were injected by a vascular route in an abdominal skin flap in nude mice. Osteosarcoma cells were fluorescently imaged in the epigastric cranialis vein in the abdominal skin flap by confocal microscopy. Collagen fibers were imaged in reflectance mode. At early stages of tumor embolus-formation, cancer cells adhered firmly to each other, diffusely expressing αvintegrin-GFP. Two weeks after injection, collagen fiber scaffolds were visualized at the margins of tumor emboli or within them. Four weeks after injection, cancer cells invading from emboli were strongly expressing αvintegrin-GFP, and were aligned along collagen fibers. The results suggest αvintegrin and collagen fiber scaffolds are important for tumor embolus formation, which are potential seeds of metastasis. J. Cell. Biochem. 118: 26-30, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111.|Department of Surgery, University of California, 200 West Arbor Drive, San Diego, California 92103.|Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0125, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Tome
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111.|Department of Surgery, University of California, 200 West Arbor Drive, San Diego, California 92103.
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.affiliation: Department of Physiology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan.
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Sugimoto
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111.|Department of Surgery, University of California, 200 West Arbor Drive, San Diego, California 92103.
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Mii
authors.affiliation: Department of Surgery, University of California, 200 West Arbor Drive, San Diego, California 92103.
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0125, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kanaya
authors.affiliation: AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111.|Department of Surgery, University of California, 200 West Arbor Drive, San Diego, California 92103.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D058948:Cell Tracking / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D008819:Mice, Nude; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D039482:Integrin alpha5; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
keywords: COLLAGEN FIBER SCAFFOLD; CONFOCAL MICROSCOPY; GREEN FLUORESCENT PROTEIN; OSTEOSARCOMA; REAL-TIME IMAGING; αv INTEGRIN