title: Induction of wheat straw delignification by Trametes species.
abstract: Wheat straw is the major crop residue in European countries which makes it the most promising material for bioconversion into biofuels. However, cellulose and hemicellulose are protected with lignin, so delignification is an inevitable phase in lignocellulose processing. The organisms predominantly responsible for its degradation are white-rot fungi and among them Trametes species represent promising degraders due to a well-developed ligninolytic enzyme system. Although numerous studies have confirmed that low molecular weight compounds can induce the production and activity of ligninolytic enzymes it is not clear how this reflects on the extent of delignification. The aim of the study was to assess the capacity of p-anisidine and veratryl alcohol to induce the production and activity of Mn-oxidizing peroxidases and laccases, and wheat straw delignification by six Trametes species. Significant inter- and intraspecific variations in activity and features of these enzymes were found, as well as differences in the potential of lignocellulose degradation in the presence or absence of inducers. Differences in the catalytic properties of synthesized enzyme isoforms strongly affected lignin degradation. Apart from enhanced lignin degradation, the addition of p-anisidine could significantly improve the selectivity of wheat straw ligninolysis, which was especially evident for T. hirsuta strains.
authors.affiliation: University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Knežević
authors.affiliation: University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Stajić
authors.affiliation: University of Belgrade, Institute for Biological Research "Siniša Stanković" Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Jovanović
authors.affiliation: University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
authors.forename: Višnja
authors.initials: V
authors.lastname: Kovačević
authors.affiliation: University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Ćilerdžić
authors.affiliation: University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Milovanović
authors.affiliation: University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Vukojević
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism; D001592:Benzyl Alcohols / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D042845:Laccase / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry*; D010544:Peroxidases / Q000378:metabolism*; D059748:Proteolysis; D055454:Trametes / Q000201:enzymology / Q000502:physiology*; D014908:Triticum / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D001592:Benzyl Alcohols; D005656:Fungal Proteins; C036909:lignocellulose; C013813:4-anisidine; D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; C042197:veratryl alcohol