title: Development and characterization of 15 microsatellite markers for Cephalotaxus fortunei (Cephalotaxaceae).
abstract: OBJECTIVE
  To survey population variation and the adaptive evolution of Cephalotaxus fortunei (Cephalotaxaceae), an endemic and endangered conifer in China, microsatellite markers were developed and characterized for this species.
  
  
  RESULTS
  Based on the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 15 microsatellite markers were developed for C. fortunei, 13 of which were polymorphic within a sample of 75 individuals representing five natural populations. The number of alleles per locus ranged from one to seven. The expected and observed heterozygosities were 0.108-0.738 and 0.000-1.000, respectively. Ten polymorphic loci were also successfully amplified in C. oliveri.
  
  
  CONCLUSIONS
  These polymorphic loci provide a valuable tool for population genetic analysis of C. fortunei, which will contribute to its management and conservation.
authors.affiliation: Department of Life Sciences, Qiannan Normal College for Nationalities, Duyun 558000, People's Republic of China.
authors.forename: Chunbo
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Life Sciences, Qiannan Normal College for Nationalities, Duyun 558000, People's Republic of China.
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Life Sciences, Qiannan Normal College for Nationalities, Duyun 558000, People's Republic of China.
authors.forename: Xilian
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
keywords: Cephalotaxaceae; Cephalotaxus fortunei; FIASCO; cross-amplification; genetic analysis; microsatellite primers