title: Interleukin 10 and transforming growth factor beta 1 gene polymorphisms in juvenile idiopathic arthritis.
abstract: OBJECTIVE
  The aim of this study is to identify the associations between interleukin 10 (IL-10) and transforming growth factor beta 1 (TGF-β1) gene polymorphisms and individual susceptibility to juvenile idiopathic arthritis (JIA) in a group of Iranian patients.
  
  
  BACKGROUND
  Cytokine genes, including IL-10 and TGF-β1, are known to play important roles in the pathogenesis of JIA.
  
  
  METHODS
  Using polymerase chain reaction with sequence-specific primers method, the frequency of alleles, genotypes and haplotypes of IL-10 (positions -1082, -819, -592) and TGF-β1 (codon 10, codon 25) single-nucleotide polymorphisms (SNPs) were investigated in 55 patients with JIA as a case group and compared with 140 healthy unrelated controls.
  
  
  RESULTS
  The G allele was significantly less frequent at TGF-β1 codon 25 in patients with JIA than in the controls (p < 0.01). The frequency of CT genotype at TGF-β1 codon 10 was found to be higher in healthy individuals in comparison with that in patients group (p = 0.04). We observed no differences in the frequency of alleles, genotypes and haplotypes of IL-10 gene between the groups of patients and controls.
  
  
  CONCLUSIONS
  Considering the low frequency of existence of TGF-β1 G allele at codon 25 as well as TGF-β1 CT genotype at codon 10 in patients with JIA, it seems that these cytokine gene polymorphisms could play role as the protective factors against JIA.
authors.forename: S
authors.initials: S
authors.lastname: Harsini
authors.forename: V
authors.initials: V
authors.lastname: Ziaee
authors.forename: M
authors.initials: M
authors.lastname: Maddah
authors.forename: A
authors.initials: A
authors.lastname: Rezaei
authors.forename: M
authors.initials: M
authors.lastname: Sadr
authors.forename: S
authors.initials: S
authors.lastname: Zoghi
authors.forename: M H
authors.initials: MH
authors.lastname: Moradinejad
authors.forename: F
authors.initials: F
authors.lastname: Tahghighi
authors.forename: Y
authors.initials: Y
authors.lastname: Aghighi
authors.forename: N
authors.initials: N
authors.lastname: Rezaei
mesh_terms: D000483:Alleles; D001171:Arthritis, Juvenile / Q000235:genetics*; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007492:Iran; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D016207:Cytokines; C508609:IL10 protein, human; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10
keywords: children.; interleukin 10; juvenile idiopathic arthritis; single‑nucleotide polymorphism; transforming growth factor beta 1