title: Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial.
abstract: Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by (1)H - nuclear magnetic resonance spectroscopy and matrix - assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(β1-3)Gal(β1-4)Glc, Gal(β1-3)Gal(β1-3)Gal(β1-4)Glc, Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc, Gal(β1-3)Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc, Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-3)Gal(β1-4)Glc, Gal(β1-3)[Gal(β1-3)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc, Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc, Neu5Ac(α2-3) Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc, Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc with an α(2-3)Neu5Ac linked to β(1-4)Gal residue of either branch of Gal(β1-4)GlcNAc(β1-6) units, and Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc with a β(1-3) linked Gal and an α(2-3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(β1-3){Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)GlcNAc(β1-6)}Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc and Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-3){Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)GlcNAc(β1-6)}Gal(β1-4)Glc and their α(2-3) linked Neu5Ac derivatives.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Hokkaido, Obihiro, 080-8555, Japan. urashima@obihiro.ac.jp.
authors.forename: Tadasu
authors.initials: T
authors.lastname: Urashima
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Hokkaido, Obihiro, 080-8555, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Hokkaido, Obihiro, 080-8555, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hirayama
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Hokkaido, Obihiro, 080-8555, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuda
authors.affiliation: Department of Food Science, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Graduate School of Agriculture, Tohoku University, Sendai, 981-8555, Japan.
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
authors.affiliation: , 13 Pepper Street,, Magill, SA, 5072, Australia.
authors.forename: Keith
authors.initials: K
authors.lastname: Newgrain
authors.affiliation: , 20 Templeton St, Cook, ACT, 2614, Australia.
authors.forename: Jim
authors.initials: J
authors.lastname: Merchant
authors.affiliation: Institute for Applied Ecology, University of Canberra, ACT, Canberra, 2601, Australia.
authors.forename: Brian
authors.initials: B
authors.lastname: Green
authors.affiliation: School of Molecular Biosciences, The University of Sydney, NSW, Sydney, 2006, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Messer
mesh_terms: D000818:Animals; D005260:Female; D008394:Marsupialia / Q000378:metabolism*; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism
chemical_list: D009844:Oligosaccharides
keywords: Marsupials; Milk oligosaccharides; Tiger quoll