title: Equine schlafen 11 restricts the production of equine infectious anemia virus via a codon usage-dependent mechanism.
abstract: Human schlafen11 is a novel restriction factor for HIV-1 based on bias regarding relative synonymous codon usage (RSCU). Here, we report the cloning of equine schlafen11 (eSLFN11) and the characteristics of its role in restricting the production of equine infectious anemia virus (EIAV), a retrovirus similar to HIV-1. Overexpression of eSLFN11 inhibited EIAV replication, whereas knockdown of endogenous eSLFN11 by siRNA enhanced the release of EIAV from its principal target cell. Notably, although eSLFN11 significantly suppressed expression of viral Gag protein and EIAV release into the culture medium, the levels of intracellular viral early gene proteins Tat and Rev and viral genomic RNA were unaffected. Coincidently, similar altered patterns of codon usage bias were observed for both the early and late genes of EIAV. Therefore, our data suggest that eSLFN11 restricts EIAV production by impairing viral mRNA translation via a mechanism that is similar to that employed by hSLFN11 for HIV-1.
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China. Electronic address: sndhr@163.com.
authors.forename: Yue-Zhi
authors.initials: YZ
authors.lastname: Lin
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China. Electronic address: sunlk0916@126.com.
authors.forename: Liu-Ke
authors.initials: LK
authors.lastname: Sun
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China; College of Wildlife Resources, Northeast Forestry University, Harbin, China. Electronic address: zhudantong123@163.com.
authors.forename: Dan-Tong
authors.initials: DT
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China. Electronic address: huzher@126.com.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China. Electronic address: xuefengwang1982@126.com.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China. Electronic address: chengdu1981@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Du
authors.affiliation: Department of Geriatrics And Gerontology, First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: wang_yu_hong@163.com.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China. Electronic address: xjw@hvri.ac.cn.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China. Electronic address: jianhua_uc@126.com.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003062:Codon; D015967:Gene Expression Regulation, Viral; D055785:Gene Knockdown Techniques; D016679:Genome, Viral; D015497:HIV-1 / Q000502:physiology; D006736:Horses; D006801:Humans; D004860:Infectious Anemia Virus, Equine / Q000502:physiology*; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D012367:RNA, Viral; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D057074:Virus Release; D014779:Virus Replication
chemical_list: D003062:Codon; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D012367:RNA, Viral; D014764:Viral Proteins
keywords: Equine infectious anemia virus; Relative synonymous codon usage; SLFN11