title: PCBP2 Modulates Neural Apoptosis and Astrocyte Proliferation After Spinal Cord Injury.
abstract: PCBP2, a member of the poly(C)-binding protein (PCBP) family, plays a pivotal role in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs in target mRNAs. It is reported that several PCBP family members are involved in human malignancies. However, the distribution and function of PCBP2 in the central nervous system (CNS) remain unclear. In this study, we performed an acute spinal cord injury (SCI) model in adult rats and investigated the dynamic changes of PCBP2 expression in the spinal cord. Western blot and immunohistochemistry analysis revealed that PCBP2 presented in normal spinal cord. It gradually increased, reached a peak at 3 day, and then declined to basal levels at 14 days after SCI. We observed that the expression of PCBP2 was enhanced in the gray and white matter. Immunofluorescence indicated that PCBP2 was located in the neurons and astrocytes. Moreover, colocalization of PCBP2/active caspase-3 was detected in neurons, and colocalization of PCBP2/proliferating cell nuclear antigen was detected in astrocytes after SCI. These results indicated that PCBP2 might play an important role in neuronal apoptosis and astrocyte proliferation. In vitro, PCBP2-specific siRNA-transfected neuron showed significantly decrease of neuronal apoptosis and expression of cell cycle related proteins following glutamate stimulation. Meanwhile, PCBP2 knockdown also reduced primary astrocytes proliferation. All above indicated that PCBP2 might play a crucial role in cell proliferation and apoptosis. Collectively, our data suggested that PCBP2 might play important roles in CNS pathophysiology after SCI.
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qian
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Rehabilitation, Second Peoples Hospital of Nantong, Nantong, 226001, Jiangsu Province, China.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Rehabilitation, Second Peoples Hospital of Nantong, Nantong, 226001, Jiangsu Province, China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Rehabilitation, Second Peoples Hospital of Nantong, Nantong, 226001, Jiangsu Province, China.
authors.forename: Chengjin
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China. docwangyh@163.com.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001253:Astrocytes / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D008297:Male; D009474:Neurons / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D016601:RNA-Binding Proteins; C571107:poly(rC) binding protein 2, rat; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Apoptosis; Astrocyte; Neuron; PCBP2; Proliferation; Spinal cord injury