title: Enhancing immune responses of EV71 VP1 DNA vaccine by co-inoculating plasmid IL-12 or GM-CSF expressing vector in mice.
abstract: Enterovirus 71 (EV71) is a major causative viral agent for large outbreaks of hand, foot, and mouth disease in children and infants, yet there is no vaccine or effective antiviral treatment for severe EV71 infection. The immunogenicity of EV71 VP1 DNA vaccine and the immunoregulatory activity of interleukin-12 (IL-12) or granulocyte-monocyte colony stimulating factor (GM-CSF) were investigated. DNA vaccine plasmids, pcDNA-VP1, pcDNA-IL-12 and pcDNA-GM-CSF were constructed and inoculated into BALB/c mice with or without pcDNA-IL-12 or pcDNA-GM-CSF by intramuscular injection. Cellular and humoral immune responses were assessed by indirect ELISA, lymphocyte proliferation assays, cytokine release assay and FACS. The VP1 DNA vaccine had good immunogenicity and can induce speciﬁc humoral and cellular immunity in BALB/c mice, while IL-2 or GM-CSF plays an immunoadjuvant role and enhances speciﬁc immune responses. This study provides a frame of reference for the design of DNA vaccines against EV71.
authors.affiliation: Nanchang University Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of life science Nanchang China.
authors.forename: X
authors.initials: X
authors.lastname: Peng
authors.affiliation: Tongji University School of Medicine Department of Immunology and Pathogen Biology Shanghai China fxiaonan@tongji.edu.cn.
authors.forename: X
authors.initials: X
authors.lastname: Fang
authors.affiliation: Tongji University School of Medicine Department of Biochemistry Shanghai China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangxi Agricultural University College of Bioscience and Engineering Nanchang China.
authors.forename: L
authors.initials: L
authors.lastname: Kong
authors.affiliation: City of Hope Department of Molecular Medicine USA.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.affiliation: Nanchang University Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of life science Nanchang China.
authors.forename: X
authors.initials: X
authors.lastname: Ding
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D029821:Enterovirus A, Human / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D007109:Immunity; D018664:Interleukin-12 / Q000276:immunology*; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000008:administration & dosage*; D013154:Spleen / Q000166:cytology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines; D018664:Interleukin-12; D016178:Granulocyte-Macrophage Colony-Stimulating Factor