title: Air-dried cells from the anhydrobiotic insect, Polypedilum vanderplanki, can survive long term preservation at room temperature and retain proliferation potential after rehydration.
abstract: Pv11, a cell line derived from the anhydrobiotic insect, Polypedilum vanderplanki, was preserved in a dry form (only 6% residual moisture) at room temperature for up to 251 days and restarted proliferating after rehydration. A previous study already reported survival of Pv11 cells after desiccation, but without subsequent proliferation. Here, the protocol was improved to increase survival and achieve proliferation of Pv11 cells after dry storage. The method basically included preincubation, desiccation and rehydration processes and each step was investigated. So far, preincubation in a 600 mM trehalose solution for 48 h before dehydration was the most favourable preconditioning to achieve successful dry preservation of Pv11 cells, allowing about 16% of survival after rehydration and subsequent cell proliferation. Although the simple air-dry method established for Pv11 cells here was not applicable for successful dry-preservation of other insect cell lines, Pv11 is the first dry-preservable animal cell line and will surely contribute not only to basic but also applied sciences.
authors.affiliation: Institute of Agrobiological Sciences, NARO, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634, Japan.
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Institute of Agrobiological Sciences, NARO, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Imanishi
authors.affiliation: Institute of Agrobiological Sciences, NARO, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634, Japan.
authors.forename: Gaku
authors.initials: G
authors.lastname: Akiduki
authors.affiliation: Institute of Agrobiological Sciences, NARO, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634, Japan.
authors.forename: Richard
authors.initials: R
authors.lastname: Cornette
authors.affiliation: Institute of Agrobiological Sciences, NARO, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634, Japan. Electronic address: oku@affrc.go.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okuda
mesh_terms: D000818:Animals; D002460:Cell Line; D002683:Chironomidae / Q000502:physiology*; D003890:Desiccation / Q000379:methods*; D007814:Larva / Q000378:metabolism; D011309:Preservation, Biological / Q000379:methods*; D013696:Temperature
keywords: Air-dry method; Anhydrobiosis; Animal cell; Desiccation tolerance; Dry-preservation; Insect cell line; Polypedilum vanderplanki; Preincubation; Trehalose