title: Explorative study of apple juice fluorescence in relation to antioxidant properties.
abstract: Total fluorescence spectra (excitation-emission matrices, EEM) were recorded for a series of commercial apple juices, including clear and cloudy juices produced from concentrate, cloudy juices that were not from concentrate, and freshly squeezed juices. An exploratory study of the spectra with parallel factor analysis (PARAFAC) revealed three groups of fluorophores with different emission properties, and these properties were characterized by excitation/emission maxima at 270/315nm, (310, 370)/455nm, and 430/(550, 680)nm, respectively. A regression analysis of the total fluorescence spectra arranged into three-way arrays using N-way partial least squares regression methods (NPLS1 and NPLS2) and an analysis of the unfolded spectra by partial least squares methods (PLS1 and PLS2) revealed quantitative relations between the fluorescence and antioxidant properties of juices. The best models for the total phenolic contents and total antioxidant capacities were obtained by applying the NPLS1 method to the EEM. The model parameters were as follows: R(2)CV=0.802, RPD=2.3 for the total phenolic content and R(2)CV=0.808 and RPD=2.3 for the total antioxidant capacity. These results show the potential use of fluorescence spectroscopy for screening apple juices for their antioxidant properties.
authors.affiliation: Faculty of Commodity Science, Poznań University of Economics and Business, al. Niepodległości 10, 60-967 Poznań, Poland. Electronic address: katarzyna.wlodarska@ue.poznan.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Włodarska
authors.affiliation: Faculty of Commodity Science, Poznań University of Economics and Business, al. Niepodległości 10, 60-967 Poznań, Poland. Electronic address: kasia.lemanska@ue.poznan.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pawlak-Lemańska
authors.affiliation: Universidade do Algarve, FCT, DQB and CIQA, Campus de Gambelas, Faro 8005-139, Portugal. Electronic address: ikhmelin@gmail.com.
authors.forename: Igor
authors.initials: I
authors.lastname: Khmelinskii
authors.affiliation: Faculty of Commodity Science, Poznań University of Economics and Business, al. Niepodległości 10, 60-967 Poznań, Poland. Electronic address: ewa.sikorska@ue.poznan.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sikorska
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D005453:Fluorescence; D005638:Fruit / Q000737:chemistry*; D027845:Malus / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000975:Antioxidants; D010636:Phenols
keywords: Antioxidants; Apple juice; Fluorescence; Multivariate regression; Parallel factor analysis