title: A dual-color fluorescent biosensing platform based on WS2 nanosheet for detection of Hg(2+) and Ag(.).
abstract: In this work, an effective dual-color fluorescent biosensing platform based on WS2 nanosheets was developed for homogeneous detection of Hg(2+) and Ag(+). This sensing platform was constituted by exploiting the fluorescence quenching ability of WS2 nanosheets and the interactions between WS2 nanosheets and DNA molecules. In the absence of additional any masking agents, the biosensor could achieve detection of Hg(2+) and Ag(+) in the same solution by monitoring fluorescence intensity changes at 525nm and 583nm, respectively. Hg(2+) and Ag(+) were selectively detected in the concentration range from 6.0-650.0nM and 5.0-1000.0nM, respectively, with the detection limit of 3.3nM and 1.2nM, respectively. It was also demonstrated that the WS2 nanosheet-based sensing platform was suitable for the simultaneous detection of Hg(2+) and Ag(+) in drinking water, serum and cell lysate samples. Moreover, the possible mechanism of fluorescence quenching by WS2 nanosheets was revealed to be related to static quenching, dynamic quenching, and Fo¨rster resonant energy transfer (FRET). This work extended the application of WS2 nanosheets to environmental monitoring and medical diagnosis.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Sensor and Sensor Technology of Gansu Province, Lanzhou 730000, China; Institute of Sensor Technology, Gansu Academy of Sciences, Lanzhou 730000, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Zuo
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Huige
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry; D060766:Drinking Water / Q000032:analysis; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis* / Q000097:blood; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver / Q000032:analysis* / Q000097:blood; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013440:Sulfides / Q000737:chemistry; D017973:Tungsten Compounds / Q000737:chemistry*
chemical_list: D060766:Drinking Water; D005456:Fluorescent Dyes; D013440:Sulfides; D017973:Tungsten Compounds; D012834:Silver; D004247:DNA; D008628:Mercury
keywords: Ag(+); Dual-color fluorescent biosensor; Hg(2+); Homogeneous detection; WS(2) nanosheet