title: Transcriptomic Analysis Reveals Adaptive Responses of an Enterobacteriaceae Strain LSJC7 to Arsenic Exposure.
abstract: Arsenic (As) resistance determinant ars operon is present in many bacteria and has been demonstrated to enhance As(V) resistance of bacteria. However, whole molecular mechanism adaptations of bacteria in response to As(V) stress remain largely unknown. In this study, transcriptional profiles of Enterobacteriaceae strain LSJC7 responding to As(V) stress were analyzed using RNA-seq and qRT-PCR. As expected, genes involved in As(V) uptake were down-regulated, those involved in As(V) reduction and As(III) eﬄux were up-regulated, which avoided cellular As accumulation. Reactive oxygen species and nitric oxide (NO) were induced, which caused cellular damages including DNA, protein, and Fe-S cluster damage in LSJC7. The expression of specific genes encoding transcriptional regulators, such as nsrR and soxRS were also induced. NsrR and SoxRS modulated many critical metabolic activities in As(V) stressed LSJC7 cells, including reactive species scavenging and repairing damaged DNA, proteins, and Fe-S clusters. Therefore, besides As uptake, reduction, and eﬄux; oxidative stress defense and damage repair were the main cellular adaptive responses of LSJC7 to As(V) stress.
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Yingjiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesBeijing, China; University of Chinese Academy of SciencesBeijing, China.
authors.forename: Songcan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Hao
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Su
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Ximei
authors.initials: X
authors.lastname: Xue
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of SciencesXiamen, China; State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesBeijing, China.
authors.forename: Yong-Guan
authors.initials: YG
authors.lastname: Zhu
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences Xiamen, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ye
keywords: Enterobacteriaceae; RNA-seq; arsenic; nitric oxide; reactive oxygen species; transcriptome