title: Enrichment and identification of biosurfactant-producing oil field microbiota utilizing electron acceptors other than oxygen and nitrate.
abstract: Microorganisms indigenous to an oil reservoir were grown in media containing either sucrose or proteins in four steel vessels under anoxic conditions at 30°C and 8.3MPa for 30days, to enrich biosurfactant producers. Fermentation of substrate was possible in the protein-containing medium and either fermentation or respiration through reduction of sulfate occurred in the sucrose-containing medium. Growth of microorganisms led to 3.4-5.4-fold surface tension reduction indicating production of biosurfactants in amounts sufficient for enhancement of gas-driven oil recovery. Analysis of sequenced cpn60 amplicons showed that Pseudomonas sp. highly similar to biosurfactant producing P. fluorescens and to Pseudomonas sp. strain TKP predominated, and a bacterium highly similar to biosurfactant producing Bacillus mojavensis was present in vessels. Analysis of 16S rDNA amplicons allowed only genus-level identification of these bacteria. Thus, cpn60-amplicon analysis was a more relevant tool for identification of putative biosurfactant producers than 16S rDNA-amplicon analysis.
authors.affiliation: National Research Council Canada (NRC-CNRC), 110 Gymnasium Pl., Saskatoon, SK S7N 0W9, Canada. Electronic address: ymkryach@hotmail.ca.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Kryachko
authors.affiliation: Dycor Technologies Ltd., 1851-94St., Edmonton, AB T6N 1E6, Canada.
authors.forename: Diana
authors.initials: D
authors.lastname: Semler
authors.affiliation: Dycor Technologies Ltd., 1851-94St., Edmonton, AB T6N 1E6, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Vogrinetz
authors.affiliation: Dycor Technologies Ltd., 1851-94St., Edmonton, AB T6N 1E6, Canada.
authors.forename: Markus
authors.initials: M
authors.lastname: Lemke
authors.affiliation: Department of Computer Science, University of Saskatchewan, 176 Thorvaldson Bldg., 110 Science Pl., University of Saskatchewan, Saskatoon, SK S7N 5C9, Canada; Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Pl., Saskatoon, SK S7N 0X2, Canada.
authors.forename: Matthew G
authors.initials: MG
authors.lastname: Links
authors.affiliation: National Research Council Canada (NRC-CNRC), 110 Gymnasium Pl., Saskatoon, SK S7N 0W9, Canada.
authors.forename: E Luke
authors.initials: EL
authors.lastname: McCarthy
authors.affiliation: National Research Council Canada (NRC-CNRC), 110 Gymnasium Pl., Saskatoon, SK S7N 0W9, Canada.
authors.forename: Brenda
authors.initials: B
authors.lastname: Haug
authors.affiliation: National Research Council Canada (NRC-CNRC), 110 Gymnasium Pl., Saskatoon, SK S7N 0W9, Canada.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Hemmingsen
mesh_terms: D020640:Arcobacter / Q000235:genetics; D001407:Bacillus / Q000235:genetics; D019149:Bioreactors / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004583:Electrons; D005285:Fermentation; D059013:Microbial Consortia / Q000235:genetics* / Q000502:physiology*; D061329:Oil and Gas Fields / Q000382:microbiology*; D011549:Pseudomonas / Q000235:genetics; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D013501:Surface-Active Agents
keywords: Interfacial tension; Microbial community; Microbially enhanced oil recovery (MEOR); cpn60