title: Characterization of RNA-binding properties of the archaeal Hfq-like protein from Methanococcus jannaschii.
abstract: The Sm and Sm-like proteins are widely distributed among bacteria, archaea and eukarya. They participate in many processes related to RNA-processing and regulation of gene expression. While the function of the bacterial Lsm protein Hfq and eukaryotic Sm/Lsm proteins is rather well studied, the role of Lsm proteins in Archaea is investigated poorly. In this work, the RNA-binding ability of an archaeal Hfq-like protein from Methanococcus jannaschii has been studied by X-ray crystallography, anisotropy fluorescence and surface plasmon resonance. It has been found that MjaHfq preserves the proximal RNA-binding site that usually recognizes uridine-rich sequences. Distal adenine-binding and lateral RNA-binding sites show considerable structural changes as compared to bacterial Hfq. MjaHfq did not bind mononucleotides at these sites and would not recognize single-stranded RNA as its bacterial homologues. Nevertheless, MjaHfq possesses affinity to poly(A) RNA that seems to bind at the unstructured positive-charged N-terminal tail of the protein.
authors.affiliation: a Institute of Protein Research , Russian Academy of Sciences , Pushchino , Moscow region , 142290 , Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Nikulin
authors.affiliation: a Institute of Protein Research , Russian Academy of Sciences , Pushchino , Moscow region , 142290 , Russia.
authors.forename: Alisa
authors.initials: A
authors.lastname: Mikhailina
authors.affiliation: a Institute of Protein Research , Russian Academy of Sciences , Pushchino , Moscow region , 142290 , Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Lekontseva
authors.affiliation: a Institute of Protein Research , Russian Academy of Sciences , Pushchino , Moscow region , 142290 , Russia.
authors.forename: Vitalii
authors.initials: V
authors.lastname: Balobanov
authors.affiliation: a Institute of Protein Research , Russian Academy of Sciences , Pushchino , Moscow region , 142290 , Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Nikonova
authors.affiliation: a Institute of Protein Research , Russian Academy of Sciences , Pushchino , Moscow region , 142290 , Russia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Tishchenko
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D035001:Host Factor 1 Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D063747:Methanocaldococcus / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D011061:Poly A / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D019642:RNA, Archaeal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D019843:Archaeal Proteins; D035001:Host Factor 1 Protein; D019642:RNA, Archaeal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D011061:Poly A
keywords: Hfq; Lsm proteins; RNA–protein interactions; archaea; crystal structure; translation regulation