title: The human placental proteome is affected by maternal smoking.
abstract: Detrimental effects of maternal smoking on the term placental proteome and steroid-metabolizing activities, and maternal hormone levels, were studied by using seven non-smoker and seven smoker placentae. Smoking significantly affected 18% of protein spots. The functional networks affected were i) cell morphology, cellular assembly and organization, cellular compromise (15 hits) and ii) DNA replication, recombination, and repair, energy production, nucleic acid metabolism (6 hits). Smoking significantly up-regulated such proteins as, SERPINA1, EFHD1 and KRT8; and down-regulated SERPINB2, FGA and HBB. Although maternal plasma steroids were not significantly altered, the catalytic activity of CYP1A1 was increased whereas CYP19A1 activity was reduced by smoking. Furthermore, transcript expression of CYP1A1 and CYP4B1 were induced while HSD17B2, NFKB and TGFB1 were repressed by smoking. The observed smoking induced wide-spread changes on placental proteome and transcript levels may contribute to the lowered birth weights of the new-born child and placenta.
authors.affiliation: School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211, Kuopio, Finland.
authors.forename: Pasi
authors.initials: P
authors.lastname: Huuskonen
authors.affiliation: Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Amezaga
authors.affiliation: Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G61 1QH, UK.
authors.forename: Michelle
authors.initials: M
authors.lastname: Bellingham
authors.affiliation: Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
authors.forename: Lucy H
authors.initials: LH
authors.lastname: Jones
authors.affiliation: School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211, Kuopio, Finland.
authors.forename: Markus
authors.initials: M
authors.lastname: Storvik
authors.affiliation: School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211, Kuopio, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Häkkinen
authors.affiliation: Department of Obstetrics and Gynaecology, Kuopio University Hospital, FIN-70211 Kuopio, Finland.
authors.forename: Leea
authors.initials: L
authors.lastname: Keski-Nisula
authors.affiliation: Department of Obstetrics and Gynaecology, Kuopio University Hospital, FIN-70211 Kuopio, Finland.
authors.forename: Seppo
authors.initials: S
authors.lastname: Heinonen
authors.affiliation: Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G61 1QH, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: O'Shaughnessy
authors.affiliation: Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Fowler
authors.affiliation: School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211, Kuopio, Finland. Electronic address: markku.pasanen@uef.fi.
authors.forename: Markku
authors.initials: M
authors.lastname: Pasanen
mesh_terms: D000328:Adult; D001141:Aromatase / Q000378:metabolism; D001724:Birth Weight; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D005260:Female; D014453:Glucuronosyltransferase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D009035:Mothers; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D020543:Proteome; D012907:Smoking / Q000378:metabolism*; D013256:Steroids / Q000097:blood; D055815:Young Adult
chemical_list: D020543:Proteome; D013256:Steroids; D001141:Aromatase; D019363:Cytochrome P-450 CYP1A1; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase
keywords: Foetus; Maternal smoking; Metabolism; Placenta; Proteomics; Steroid hormones