title: Association of Interleukin-27 rs 153109 Single Nucleotide Polymorphism with Spontaneous Resolution of Hepatitis C Virus - Genotype 4a Infection in Egyptian Patients.
abstract: BACKGROUND
  HCV is a major global health problem. IL-27 is a member of the IL-6/IL-12 cytokine family with a broad range of anti-inflammatory properties. Recent studies highlighted the effect of a SNP in the IL-27 promoter region on modulating the progression of infectious diseases and individual responses to therapy.
  
  
  OBJECTIVE
  The present study investigated the potential role of (-964 A/G) SNP in the promoter region of IL-27p28 gene (alleles rs153109) on the outcome of HCV infection among genotype 4a infected patients.
  
  
  METHODS
  HCV genotyping confirmed that all of the HCV-infected patients had genotype 4a infection. Genomic DNA was extracted from 111 patients with chronic HCV infection, 42 spontaneous resolvers (SR) and 16 healthy controls. IL- 27p28.rs153109 genotyping was assessed using PCR-RFLP then confirmed by DNA sequencing.
  
  
  RESULTS
  The frequency of IL-27-p28.rs153109AA, AG, and GG genotypes among chronically infected subjects were 74.8 %, 25.2%, and 0% while among the SR, they were 57.1%, 35.7%, and 7.14%, respectively. Our data show the unique presence of G/G genotype in the SR group (3 patients; 7.14%). Moreover, the "G" allele frequencies among chronic and resolved subjects were 12.6% and 25.0%, respectively (p=0.0136). Importantly, subjects with the GG genotype were more likely to clear their HCV infection than those with the AA genotype (p=0.0118).
  
  
  CONCLUSIONS
  HCV genotype 4a subjects with the IL-27-p28.rs153109 A/G and G/G genotype were more likely to clear their HCV infection. Therefore, we propose IL- 27p28.rs153109SNPas a genetic biomarker for predicting HCV infection outcome.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Minia, Egypt E-mail : ahmed_wahid@ mu.edu.eg.
authors.forename: Mariam M
authors.initials: MM
authors.lastname: Fawzy
authors.forename: Ahmed
authors.initials: A
authors.lastname: Wahid
authors.forename: Maiiada H
authors.initials: MH
authors.lastname: Nazmy
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hashem
authors.forename: Imam
authors.initials: I
authors.lastname: Waked
authors.forename: Sayed F
authors.initials: SF
authors.lastname: Abdelwahab
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000627:therapeutic use; D004534:Egypt; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000188:drug therapy / Q000235:genetics* / Q000821:virology; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D000998:Antiviral Agents; D007378:Interleukins; C464565:MYDGF protein, human; C093542:NS4 protein, hepatitis C virus; D017361:Viral Nonstructural Proteins