title: A high affinity phage-displayed peptide as a recognition probe for the detection of Salmonella Typhimurium.
abstract: Salmonellosis is one of the most common and widely distributed foodborne diseases. A sensitive and robust detection method of Salmonella Typhimurium (S. Typhimurium) in food can critically prevent a disease outbreak. In this work, the use of phage displayed peptides was explored for the detection of S. Typhimurium. A phage-displayed random dodecapeptide library was subjected to biopanning against lipopolysaccharide (LPS) of S. Typhimurium. The peptide NFMESLPRLGMH (pep49) derived from biopanning displayed a high affinity (25.8nM) for the LPS of S. Typhimurium and low cross-reactivity with other strains of Salmonella and related Gram-negative bacteria. Molecular insights into the interaction of pep49 with the LPS of S. Typhimurium was gleaned using atomistic molecular dynamics simulations and docking. It was deduced that the specificity of pep49 with S. Typhimurium LPS originated from the interactions of pep49 with abequose that is found only in the O-antigen of S. Typhimurium. Further, pep49 was able to detect S. Typhimurium at a LOD of 10(3) CFU/mL using ELISA, and may be a potential cost efficient alternative to antibodies.
authors.affiliation: Nanobioscience group, Agharkar Research Institute, GG Agarkar Road, Pune 411 004, India.
authors.forename: Shailaja
authors.initials: S
authors.lastname: Agrawal
authors.affiliation: Nanobioscience group, Agharkar Research Institute, GG Agarkar Road, Pune 411 004, India.
authors.forename: Prabir Kumar
authors.initials: PK
authors.lastname: Kulabhusan
authors.affiliation: Bioinformatics Centre, Savitribai Phule Pune University, Pune 411007, India.
authors.forename: Manali
authors.initials: M
authors.lastname: Joshi
authors.affiliation: Nanobioscience group, Agharkar Research Institute, GG Agarkar Road, Pune 411 004, India.
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Bodas
authors.affiliation: Nanobioscience group, Agharkar Research Institute, GG Agarkar Road, Pune 411 004, India. Electronic address: kpaknikar@gmail.com.
authors.forename: Kishore M
authors.initials: KM
authors.lastname: Paknikar
mesh_terms: D057230:Limit of Detection; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D062105:Molecular Docking Simulation; D058889:Molecular Typing; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D019151:Peptide Library; D012486:Salmonella typhimurium / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D009842:Oligopeptides; D019151:Peptide Library
keywords: Docking; Lipopolysaccharide; Molecular dynamic studies; Phage display peptide; Salmonella Typhimurium