title: Artificial Autopolyploidization Modifies the Tricarboxylic Acid Cycle and GABA Shunt in Arabidopsis thaliana Col-0.
abstract: Autopolyploidy is a process whereby the chromosome set is multiplied and it is a common phenomenon in angiosperms. Autopolyploidy is thought to be an important evolutionary force that has led to the formation of new plant species. Despite its relevance, the consequences of autopolyploidy in plant metabolism are poorly understood. This study compares the metabolic profiles of natural diploids and artificial autotetraploids of Arabidopsis thaliana Col-0. Different physiological parameters are compared between diploids and autotetraploids using nuclear magnetic resonance (NMR), elemental analysis (carbon:nitrogen balance) and quantitative real-time PCR (qRT-PCR). The main difference between diploid and autotetraploid A. thaliana Col-0 is observed in the concentration of metabolites related to the tricarboxylic acid cycle (TCA) and γ-amino butyric acid (GABA) shunt, as shown by multivariate statistical analysis of NMR spectra. qRT-PCR shows that genes related to the TCA and GABA shunt are also differentially expressed between diploids and autotetraploids following similar trends as their corresponding metabolites. Solid evidence is presented to demonstrate that autopolyploidy influences core plant metabolic processes.
authors.affiliation: Environmental Metabolic Analysis Research Team, CSRS, RIKEN. 1-7-22 Suehiro-cho, Tsurumi-ku, Kanagawa-ken, 230-0045, Japan.
authors.forename: Fredd
authors.initials: F
authors.lastname: Vergara
authors.affiliation: Environmental Metabolic Analysis Research Team, CSRS, RIKEN. 1-7-22 Suehiro-cho, Tsurumi-ku, Kanagawa-ken, 230-0045, Japan.|Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Kanagawa 230-0045, Japan.|Graduate School of Bioagricultural Sciences, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya, Aichi 464-0810, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kikuchi
authors.affiliation: Cell Function Research Team, CSRS, RIKEN. 1-7-22 Suehiro-cho, Tsurumi-ku, Kanagawa-ken, 230-0045, Japan.
authors.forename: Christian
authors.initials: C
authors.lastname: Breuer
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics*; D002952:Citric Acid Cycle; D004171:Diploidy; D018506:Gene Expression Regulation, Plant; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000378:metabolism; D011123:Polyploidy; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D005680:gamma-Aminobutyric Acid; D002244:Carbon; D009584:Nitrogen