title: [A method for combining Fluoro-Jade B staining and immunofluorescent staining].
abstract: OBJECTIVE
  To explore a method for combining Fluoro-Jade B (FJB) staining with immunofluorescent staining in rats with focal cortical infarction.
  
  
  METHODS
  Permanent distal middle cerebral artery occlusion (dMCAO) was induced in rats by electrocoagulation. The rat models were randomized into two groups, and frozen sections of the brain tissues from each group were stained with FJB followed by immunofluorescent staining or in the reverse order.
  
  
  RESULTS
  FJB staining followed by immunofluorescence staining clearly visualized both FJB-positive and immunofluorescence-positive cells in the frozen sections, but the staining protocol in the reverse sequence failed to clearly show the immunofluorescence-positive cells.
  
  
  CONCLUSIONS
  FJB staining prior to immunofluorescence staining does not affect the staining effect of protein immunofluorescent staining and better visualizes the positive cells.
authors.affiliation: Institute of Neurosciences, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China.E-mail: zuo85109@163.com.
authors.forename: Xia-Lin
authors.initials: XL
authors.lastname: Zuo
authors.forename: Ji-Zi
authors.initials: JZ
authors.lastname: Jin
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Liu
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Sun
authors.forename: En
authors.initials: E
authors.lastname: Xu
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D005452:Fluoresceins / Q000737:chemistry*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D020244:Infarction, Middle Cerebral Artery; D051381:Rats; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C435731:fluoro jade