title: Integrating cell biology, image analysis, and computational mechanical modeling to analyze the contributions of cellulose and xyloglucan to stomatal function.
abstract: Cell walls are likely to be essential determinants of the amazing strength and flexibility of the guard cells that surround each stomatal pore in plants, but surprisingly little is known about cell wall composition, organization, and dynamics in guard cells. Recent analyses of cell wall organization and stomatal function in the guard cells of Arabidopsis thaliana mutants with defects in cellulose and xyloglucan have allowed for the development of new hypotheses about the relative contributions of these components to guard cell function. Advanced image analysis methods can allow for the automated detection of key structures, such as microtubules (MTs) and Cellulose Synthesis Complexes (CSCs), in guard cells, to help determine their contributions to stomatal function. A major challenge in the mechanical modeling of dynamic biological structures, such as guard cell walls, is to connect nanoscale features (e.g., wall polymers and their molecular interactions) with cell-scale mechanics; this challenge can be addressed by applying multiscale computational modeling that spans multiple spatial scales and physical attributes for cell walls.
authors.affiliation: a Department of Biology.|b Plant Biology Interdepartmental Graduate Program.
authors.forename: Yue
authors.initials: Y
authors.lastname: Rui
authors.affiliation: c Department of Agricultural and Biological Engineering.
authors.forename: Hojae
authors.initials: H
authors.lastname: Yi
authors.affiliation: d College of Information Sciences and Technology.
authors.forename: Baris
authors.initials: B
authors.lastname: Kandemir
authors.affiliation: d College of Information Sciences and Technology.
authors.forename: James Z
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: c Department of Agricultural and Biological Engineering.
authors.forename: Virendra M
authors.initials: VM
authors.lastname: Puri
authors.affiliation: a Department of Biology.|e Center for Lignocellulose Structure and Formation, The Pennsylvania State University , University Park , PA , USA.
authors.forename: Charles T
authors.initials: CT
authors.lastname: Anderson
mesh_terms: D001696:Biomechanical Phenomena; D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003198:Computer Simulation; D020342:Finite Element Analysis; D005936:Glucans / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008870:Microtubules / Q000378:metabolism; D054046:Plant Stomata / Q000166:cytology* / Q000502:physiology*; D014990:Xylans / Q000378:metabolism*
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose
keywords: Stomata; cellulose; guard cell wall; image analysis; mechanical modeling; xyloglucan