title: Involvement of DkTGA1 Transcription Factor in Anaerobic Response Leading to Persimmon Fruit Postharvest De-Astringency.
abstract: Persimmon fruit are unique in accumulating proanthocyanidins (tannins) during development, which cause astringency in mature fruit. In 'Mopanshi' persimmon, astringency can be removed by treatment with 95% CO2, which increases the concentrations of ethanol and acetaldehyde by glycolysis, and precipitates the soluble tannin. A TGA transcription factor, DkTGA1, belonging to the bZIP super family, was isolated from an RNA-seq database and real-time quantitative PCR indicated that DkTGA1 was up-regulated by CO2 treatment, in concert with the removal of astringency from persimmon fruit. Dual-luciferase assay revealed that DkTGA1 had a small (less than 2-fold), but significant effect on the promoters of de-astringency-related genes DkADH1, DkPDC2 and DkPDC3, which encode enzymes catalyzing formation of acetaldehyde and ethanol. A combination of DkTGA1 and a second transcription factor, DkERF9, shown previously to be related to de-astringency, showed additive effects on the activation of the DkPDC2 promoter. Yeast one-hybrid assay showed that DkERF9, but not DkTGA1, could bind to the DkPDC2 promoter. Thus, although DkTGA1 expression is positively associated with persimmon fruit de-astringency, trans-activation analyses with DkPDC2 indicates it is likely to act by binding indirectly DkPDC2 promoter, might with helps of DkERF9.
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.|The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Qing-Gang
authors.initials: QG
authors.lastname: Zhu
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Wang
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Zi-Yuan
authors.initials: ZY
authors.lastname: Gong
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Department of Horticultural Sciences, College of Agriculture, Guangxi University, Nanning, PR China.
authors.forename: Ning-Jing
authors.initials: NJ
authors.lastname: Sun
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.|The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.|Plant & Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom.
authors.forename: Donald
authors.initials: D
authors.lastname: Grierson
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.|The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Xue-Ren
authors.initials: XR
authors.lastname: Yin
authors.affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, PR China.|The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Kun-Song
authors.initials: KS
authors.lastname: Chen
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D029788:Diospyros / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D044945:Proanthocyanidins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D044945:Proanthocyanidins; D014157:Transcription Factors; D000431:Ethanol; D000079:Acetaldehyde; D010100:Oxygen