title: Histone H3 Dynamics Reveal Domains with Distinct Proliferation Potential in the Arabidopsis Root.
abstract: A coordinated transition from cell proliferation to differentiation is crucial for organogenesis. We found that extensive chromatin reorganization, shown here for histone H3 proteins, characterizes cell population dynamics in the root developmental compartments. The canonical H3.1 protein, incorporated during S-phase, is maintained at high levels in cells dividing at a high rate but is massively evicted in cells undergoing their last cell cycle before exit to differentiation. A similar pattern was observed in the quadruple mutant for the H3.1-encoding genes HTR1, HTR2, HTR3, and HTR9 (htr1,2,3,9), in which H3.1 is expressed only from the HTR13 gene. H3 eviction is a fast process occurring within the G2 phase of the last cell cycle, which is longer than G2 in earlier cell cycles. This longer G2 likely contributes to lower the H3.1/H3.3 ratio in cells leaving the root meristem. The high H3.1/H3.3 ratio and H3.1 eviction process also occurs in endocycling cells before differentiation, revealing a common principle of H3 eviction in the proliferating and endocycling domains of the root apex. Mutants in the H3.1 chaperone CAF-1 (fas1-4) maintain a pattern similar to that of wild-type roots. Our studies reveal that H3 incorporation and eviction dynamics identify cells with different cell division potential during organ patterning.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Sofía
authors.identifier: 0000-0001-9409-8544
authors.initials: S
authors.lastname: Otero
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Bénédicte
authors.identifier: 0000-0001-7116-9821
authors.initials: B
authors.lastname: Desvoyes
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Ramón
authors.identifier: 0000-0003-2450-2999
authors.initials: R
authors.lastname: Peiró
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, 28049 Madrid, Spain cgutierrez@cbm.csic.es.
authors.forename: Crisanto
authors.identifier: 0000-0001-8905-8222
authors.initials: C
authors.lastname: Gutierrez
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D006657:Histones / Q000378:metabolism*; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018517:Plant Roots / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones