title: A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency.
abstract: Iron (Fe) deficiency affects plant growth and development, leading to reduction of crop yields and quality. Although the regulation of Fe uptake under Fe deficiency has been well studied in the past decade, the regulatory mechanism of Fe translocation inside the plants remains unknown. Here, we show that a WRKY transcription factor WRKY46 is involved in response to Fe deficiency. Lack of WRKY46 (wrky46-1 and wrky46-2 loss-of-function mutants) significantly affects Fe translocation from root to shoot and thus causes obvious chlorosis on the new leaves under Fe deficiency. Gene expression analysis reveals that expression of a nodulin-like gene (VACUOLAR IRON TRANSPORTER1-LIKE1 [VITL1]) is dramatically increased in wrky46-1 mutant. VITL1 expression is inhibited by Fe deficiency, while the expression of WRKY46 is induced in the root stele. Moreover, down-regulation of VITL1 expression can restore the chlorosis phenotype on wrky46-1 under Fe deficiency. Further yeast one-hybrid and chromatin immunoprecipitation experiments indicate that WRKY46 is capable of binding to the specific W-boxes present in the VITL1 promoter. In summary, our results demonstrate that WRKY46 plays an important role in the control of root-to-shoot Fe translocation under Fe deficiency condition via direct regulation of VITL1 transcript levels.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (J.Y.Y., C.X.L., L.S., J.Y. R., Z.J.D., S.J.Z.); and College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China (G.X.L.).
authors.forename: Jing Ying
authors.initials: JY
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (J.Y.Y., C.X.L., L.S., J.Y. R., Z.J.D., S.J.Z.); and College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China (G.X.L.).
authors.forename: Chun Xiao
authors.initials: CX
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (J.Y.Y., C.X.L., L.S., J.Y. R., Z.J.D., S.J.Z.); and College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China (G.X.L.).
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (J.Y.Y., C.X.L., L.S., J.Y. R., Z.J.D., S.J.Z.); and College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China (G.X.L.).
authors.forename: Jiang Yuan
authors.initials: JY
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (J.Y.Y., C.X.L., L.S., J.Y. R., Z.J.D., S.J.Z.); and College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China (G.X.L.).
authors.forename: Gui Xin
authors.initials: GX
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (J.Y.Y., C.X.L., L.S., J.Y. R., Z.J.D., S.J.Z.); and College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China (G.X.L.) zjding@zju.edu.cn.
authors.forename: Zhong Jie
authors.identifier: 0000-0003-1518-5143
authors.initials: ZJ
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (J.Y.Y., C.X.L., L.S., J.Y. R., Z.J.D., S.J.Z.); and College of Agronomy and Biotechnology, Zhejiang University, Hangzhou 310058, China (G.X.L.).
authors.forename: Shao Jian
authors.identifier: 0000-0002-3336-8165
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000378:metabolism*; D009154:Mutation; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D007501:Iron; D014157:Transcription Factors; C501411:FIT1 protein, Arabidopsis; C579612:WRKY46 protein, Arabidopsis