title: [Expression of protein 4.1 family in melanoma cell lines and its effect on cell proliferation].
abstract: OBJECTIVE
  To detect the expression of protein 4.1 family members in mouse melanoma cell lines and evaluate their effect on cell proliferation.
  
  
  METHODS
  PCR and Western blot were used to detected to the expression of protein 4.1 family members (4.1R, 4.1B, 4.1G, and 4.1N) at the mRNA and protein levels in B16 and B16-F10 cell lines. The expression plasmid vector pEGFP-N1-EPB41L3 carrying 4.1B gene sequence amplified from genomic RNA of mouse embryo fibroblasts was constructed and transiently transfected into mouse melanoma cells. The change in cell proliferation was assessed using MTT assay.
  
  
  RESULTS
  The mRNA and protein expressions of all the protein 4.1 family members, with the exception of 4.1B, were detected in both B16 and B16-F10 cells. Transfection of cells with the eukaryotic expression vector pEGFP-N1-EPB41L3 markedly inhibited cell proliferation as compared with the non-transfected cells.
  
  
  CONCLUSIONS
  The eukaryotic expression vector carrying EPB41L3 sequence is capable of inhibiting the proliferation of mouse melanoma B16 and B16-F10 cells.
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou 450000, China.E-mail: menyingli@126.com.
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Men
authors.forename: Qiao-Zhen
authors.initials: QZ
authors.lastname: Kang
authors.forename: Cong
authors.initials: C
authors.lastname: Ding
authors.forename: Shi-Meng
authors.initials: SM
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wang
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Ji
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005822:Genetic Vectors; D008546:Melanoma, Experimental / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins; D009479:Neuropeptides / Q000378:metabolism*; D010957:Plasmids; D014162:Transfection
chemical_list: D003598:Cytoskeletal Proteins; C540651:Epb4.1l2 protein, mouse; C514956:Epb41 protein, mouse; C488402:Epb41l3 protein, mouse; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009479:Neuropeptides; C120557:erythrocyte membrane protein band 4.1-like 1