title: Glass substrates crosslinked with tetracycline-imprinted polymeric silicate and CdTe quantum dots as fluorescent sensors.
abstract: A fluorescence-based sensor that combines the merits of quantum dots (QDs) and molecularly imprinted polymers (MIPs) was first fabricated on a glass substrate via a sol-gel route. Some of the key performance factors, including silane selection, substrate etching, the reaction times of glass silanization and sol-gel polymerization, and the times and methods used for template stripping and loading, were discussed and determined. After fabricating the sensor on either a 3-aminopropyltriethoxysilane (APS) or a 3-mercaptopropyltriethoxysilane (MPS) modified glass substrate, APS showed a much better performance than MPS as both the capping reagent of QDs and the functional monomer of tetracycline-templated MIPs. The APS-QDs on APS-modified glass had a higher imprinted factor (IF = 5.6), a lower LOD (2.1 μM, 3σ), and a more stable signal (2.8%, n = 10 at 70 μM) than those on the MPS-modified glass (IF = 5.2, LOD = 6.5 μM, stability = 6.2%). Furthermore, the recoveries of tetracycline (70 μM) from BSA (133 μg/mL) and FBS (0.66 ppt) by the APS-modified glass were 98% (RSD = 3.5%, n = 5) and 97% (RSD = 5.7%), respectively. For the MPS-modified glass, recoveries of 95% (RSD = 7.2%) and 89% (RSD = 8.7%) were observed at 67 μg/mL of BSA and 0.33 ppt of FBS, respectively.
authors.affiliation: Department of Occupational Safety and Health, Chung Shan Medical University, Taichung 402, Taiwan; Department of Occupational Medicine, Chung Shan Medical University Hospital, Taichung 402, Taiwan.
authors.forename: Mu-Rong
authors.initials: MR
authors.lastname: Chao
authors.affiliation: Department of Public Health, Chung Shan Medical University, Taichung 402, Taiwan; Department of Family and Community Medicine, Chung Shan Medical University Hospital, Taichung 402, Taiwan.
authors.forename: Chiung-Wen
authors.initials: CW
authors.lastname: Hu
authors.affiliation: School of Pharmacy, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan. Electronic address: cjl@mail.cmu.edu.tw.
authors.forename: Jian-Lian
authors.initials: JL
authors.lastname: Chen
keywords: Fluorescence; Molecularly imprinted polymer; Quantum dots; Sol–gel; Tetracycline