title: 15-Deoxy-Δ(12,14)-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells.
abstract: The cyclopentenone prostaglandin 15-deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR-γ) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ2 decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ2 downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ2 shows the potential to become a new therapeutic approach for thyroid tumors.
authors.affiliation: Laboratory of Nanobiotechnology, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil; Laboratory of Genetics, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil; Hammock Laboratory of Pesticide Biotechnology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Carlos Antônio
authors.initials: CA
authors.lastname: Trindade-da-Silva
authors.affiliation: Laboratory of Nanobiotechnology, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil; Laboratory of Cancer Molecular Genetics, University of Campinas, 13081-970 Campinas, SP, Brazil.
authors.forename: Carolina Fernandes
authors.initials: CF
authors.lastname: Reis
authors.affiliation: Laboratory of Nanobiotechnology, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil.
authors.forename: Lara
authors.initials: L
authors.lastname: Vecchi
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, 13045-755 Campinas, SP, Brazil.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, 13045-755 Campinas, SP, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Sperandio
authors.affiliation: Laboratory of Nanobiotechnology, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil.
authors.forename: Bruna França
authors.initials: BF
authors.lastname: Matias Colombo
authors.affiliation: Laboratory of Nanobiotechnology, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil.
authors.forename: Patrícia Terra
authors.initials: PT
authors.lastname: Alves
authors.affiliation: Laboratory of Cancer Molecular Genetics, University of Campinas, 13081-970 Campinas, SP, Brazil.
authors.forename: Laura Sterian
authors.initials: LS
authors.lastname: Ward
authors.affiliation: Laboratory of Genetics, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Ueira-Vieira
authors.affiliation: Laboratory of Nanobiotechnology, Federal University of Uberlândia, 38400902 Uberlândia, MG, Brazil; Department of Medical Microbiology and Immunology, University of California Davis, Davis, CA 95616, USA.
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Goulart