title: Adipose Stromal Vascular Fraction Isolation: A Head-to-Head Comparison of 4 Cell Separation Systems #2.
abstract: BACKGROUND
  With stromal vascular fraction (SVF) cell and adipose-derived stem cell-based technologies translating into the clinical setting, numerous isolation systems have been developed for the point of care isolation of SVF cells from adipose tissue. A relative lack of performance data on these systems can make objective assessment difficult for prospective clinicians. This study compared the performance of 4 SVF cell isolation systems.
  
  
  METHODS
  Four isolation systems were compared: the MultiStation by PNC International, the LipoKit by MediKhan, the GID SVF-2 platform by GID Europe Ltd, and the StemSource 900/MB system by Cytori Therapeutics, Inc. Identical lipoaspirate samples for 5 separate donors were used. Stromal vascular fraction output was compared in terms of nucleated cell yield, viability, residual collagenase activity, sterility of the output, colony-forming unit-fibroblast frequency, frequency of CD31-/CD34+/CD45- cells, and operating statistics.
  
  
  RESULTS
  Mean process time ranged from 65.4 to 120.8 minutes. Mean nucleated cell yield per milliliter of tissue processed ranged from 1.01 × 10 cells/mL to 6.24 × 10 cells/mL. Mean cellular viability ranged from 50.3% to 84.02%. Residual collagenase activity was negligible across all systems. Observed colony-forming unit-fibroblast frequency ranged from 0.495% to 1.704%. No significant difference was observed in frequency of CD31-/CD34+/CD45- cells. Results of the anaerobic/aerobic cultures were mixed.
  
  
  CONCLUSIONS
  There was considerable variability between the outputs of each system. The system used by a clinician should be tailored to the individual needs of the practice. There is a range of cost options available. This study may help clinicians make more educated decisions when choosing an isolation system to meet their clinical needs.
authors.affiliation: From the *Cedars-Sinai Medical Center; †University Stem Cell Center; and ‡USC Keck School of Medicine, Los Angeles, CA.
authors.forename: Joel A
authors.initials: JA
authors.lastname: Aronowitz
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Lockhart
authors.forename: Cloe S
authors.initials: CS
authors.lastname: Hakakian
authors.forename: Zoe E
authors.initials: ZE
authors.lastname: Birnbaum
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D005260:Female; D006801:Humans; D015187:Lipectomy; D059630:Mesenchymal Stem Cells; D050151:Subcutaneous Fat / Q000166:cytology*