title: The molecular and clinical features of dengue during outbreak in Jambi, Indonesia in 2015.
abstract: Dengue is hyperendemic in Indonesia. In 2015, reported cases of dengue fever doubled those of 2014 in the Jambi municipality of Sumatra. We examined viral aetiology and its relationship with disease outcome in Jambi. Dengue-suspected patients' sera were collected and NS1 detection and IgM/IgG serology were performed. Dengue virus (DENV) serotyping was performed using real-time RT-PCR. Envelope genes were sequenced to determine the genotypes of DENV. Clinical, haematologic, and demographic data were recorded. Of 210 dengue-suspected patients, 107 were confirmed. The disease manifested as Dengue Fever (62%), Dengue Haemorrhagic Fever (36%), and Dengue Shock Syndrome (2%). The serotypes of 94 DENV were determined. All DENV serotypes were detected with DENV-1 as the predominant serotype (66%). Genotypically, the DENV-1 viruses belong to Genotype I, DENV-2 was of Cosmopolitan genotype, DENV-3 as Genotype I, and DENV-4 belonged to Genotype II. Comparison with historical data revealed serotype predominance switched from DENV-3 to DENV-1, and the replacement of Genotype IV of DENV-1 with Genotype I. In summary, DENV-1 predominated during the 2015 dengue outbreak in Jambi. The full spectrum of dengue disease occurred and was characterized by a switch in predominant serotypes.
authors.affiliation: a Siloam Hospital , Jambi , Indonesia.|d Faculty of Medicine , Jambi University , Jambi , Indonesia.
authors.forename: Sotianingsih
authors.initials: S
authors.lastname: Haryanto
authors.affiliation: b Eijkman Institute for Molecular Biology , Jakarta , Indonesia.
authors.forename: Rahma F
authors.initials: RF
authors.lastname: Hayati
authors.affiliation: b Eijkman Institute for Molecular Biology , Jakarta , Indonesia.
authors.forename: Benediktus
authors.initials: B
authors.lastname: Yohan
authors.affiliation: a Siloam Hospital , Jambi , Indonesia.
authors.forename: Lanceria
authors.initials: L
authors.lastname: Sijabat
authors.affiliation: a Siloam Hospital , Jambi , Indonesia.
authors.forename: Ifo F
authors.initials: IF
authors.lastname: Sihite
authors.affiliation: c Health Polytechnic , Jambi Provincial Health Office , Jambi , Indonesia.
authors.forename: Sukmal
authors.initials: S
authors.lastname: Fahri
authors.affiliation: b Eijkman Institute for Molecular Biology , Jakarta , Indonesia.
authors.forename: Febrina
authors.initials: F
authors.lastname: Meutiawati
authors.affiliation: e Faculty of Medicine , Diponegoro University , Semarang , Indonesia.
authors.forename: Jonathan A N
authors.initials: JA
authors.lastname: Halim
authors.affiliation: e Faculty of Medicine , Diponegoro University , Semarang , Indonesia.
authors.forename: Stefanie N
authors.initials: SN
authors.lastname: Halim
authors.affiliation: b Eijkman Institute for Molecular Biology , Jakarta , Indonesia.
authors.forename: Amin
authors.initials: A
authors.lastname: Soebandrio
authors.affiliation: b Eijkman Institute for Molecular Biology , Jakarta , Indonesia.
authors.forename: R Tedjo
authors.initials: RT
authors.lastname: Sasmono
mesh_terms: D000595:Amino Acid Sequence; D003715:Dengue / Q000453:epidemiology* / Q000503:physiopathology / Q000821:virology*; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D004196:Disease Outbreaks; D019353:Endemic Diseases; D062665:Epidemiological Monitoring; D019143:Evolution, Molecular; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D012703:Serotyping
keywords: Dengue; Genotype; Indonesia; Jambi; Serotype