title: The impact of the codelivery of drug-siRNA by trimethyl chitosan nanoparticles on the efficacy of chemotherapy for metastatic breast cancer cell line (MDA-MB-231).
abstract: High-mobility group protein two (HMGA2), a nonhistone nuclear-binding protein and its downregulators; vimentin, matrix metallopeptidase-9 (MMP-9), and E-cadherin are shown to contribute to tumor progression and metastasis. Thus, in this study, we checked simultaneous delivery of HMGA-2 siRNA and the anticancer drug doxorubicin to enhance the anticancer treatment effects. For this purpose, we used MTT assay and real-time polymerase chain reaction (RT-PCR). Our results showed that dual delivery of Dox and HMGA-2 siRNA by trimethyl chitosan (TMC) significantly inhibited breast cancer cells growth. Additionally, the delivery of siRNA significantly silenced HMGA-2, vimentin, and MMP9 mRNAs, but led to overexpression of E-cadherin mRNA.
authors.affiliation: a Immunology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Peyman
authors.initials: P
authors.lastname: Eivazy
authors.affiliation: d Department of Pharmaceutics, Faculty of Pharmacy, Novel Drug Delivery System Laboratory , Medical Sciences University , Tehran , Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Atyabi
authors.affiliation: e Department of Immunology, School of Public Health , Tehran University of Medical Sciences , Tabriz , Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Jadidi-Niaragh
authors.affiliation: b Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Leili
authors.initials: L
authors.lastname: Aghebati Maleki
authors.affiliation: f Department of Medical Parasitology and Mycology, School of Medicine , Alborz University of Medical Sciences , Karaj , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Miahipour
authors.affiliation: a Immunology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Jalal
authors.initials: J
authors.lastname: Abdolalizadeh
authors.affiliation: b Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.|c Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Yousefi
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D048271:Chitosan / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D020868:Gene Silencing; D025743:HMGA2 Protein / Q000172:deficiency / Q000235:genetics; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009362:Neoplasm Metastasis; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics*
chemical_list: D004337:Drug Carriers; D025743:HMGA2 Protein; D034741:RNA, Small Interfering; D004317:Doxorubicin; D048271:Chitosan
keywords: Breast cancer; HMGA2; nanoparticle; therapy