title: Watch out for your TRP1 marker: the effect of TRP1 gene on the growth at high and low temperatures in budding yeast.
abstract: TRP1 is a frequently used auxotrophic marker for genetic modifications and selections in trp(-) budding yeast strains, including the commonly used wild-type strain W303a. However, we found that introduction of the TRP1 gene into a trp(-) strain significantly affected vegetative growth at low and high temperatures. Therefore, caution should be needed when working in a trp(-) background strain and using the TRP1 marker to study stress response phenotypes, particularly when analyzing temperature sensitivities.
authors.affiliation: Department of Biochemistry, Yonsei University, College of Life Science and Biotechnology, Seoul 03722, Korea.
authors.forename: Gang
authors.initials: G
authors.lastname: Leng
authors.affiliation: Department of Biochemistry, Yonsei University, College of Life Science and Biotechnology, Seoul 03722, Korea bc5012@yonsei.ac.kr.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Song
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers; D003001:Cloning, Molecular; D003080:Cold Temperature; D006358:Hot Temperature; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D004718:Saccharomycetales / Q000235:genetics* / Q000254:growth & development*; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D015415:Biomarkers; D029701:Saccharomyces cerevisiae Proteins; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae
keywords: TRP1 gene; W303a strain; auxotrophic marker; budding yeast; high temperature; low temperature