title: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization.
abstract: Inducible dimerization is a general approach to experimentally manipulate protein-protein interactions with temporal control. This chapter describes the use of rapamycin-inducible dimerization to manipulate mitotic regulatory proteins, for example to control kinetochore localization. A significant feature of this method relative to previously described protocols is the depletion of endogenous FKBP12 protein, which markedly improves dimerization efficiency.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, PA, 19104, USA.
authors.forename: Edward R
authors.initials: ER
authors.lastname: Ballister
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, PA, 19104, USA. Lampson@sas.upenn.edu.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Lampson
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008938:Mitosis / Q000235:genetics; D011485:Protein Binding; D055503:Protein Multimerization / Q000187:drug effects*; D020123:Sirolimus / Q000494:pharmacology*
chemical_list: D018797:Cell Cycle Proteins; D020123:Sirolimus
keywords: Dimerization; Kinetochore; Rapamycin