title: The peripheral olfactory code in Drosophila larvae contains temporal information and is robust over multiple timescales.
abstract: We studied the electrophysiological activity of two classes of Drosophila melanogaster larval olfactory sensory neurons (OSNs), Or24a and Or74a, in response to 1 s stimulation with butanol, octanol, 2-heptanone, and propyl acetate. Each odour/OSN combination produced unique responses in terms of spike count and temporal profile. We used a classifier algorithm to explore the information content of OSN activity, and showed that as well as spike count, the activity of these OSNs included temporal information that enabled the classifier to accurately identify odours. The responses of OSNs during continuous odour exposure (5 and 20 min) showed that both types of neuron continued to respond, with no complete adaptation, and with no change to their ability to encode temporal information. Finally, we exposed larvae to octanol for 3 days and found only minor quantitative changes in OSN response to odours, indicating that the larval peripheral code is robust when faced with long-term exposure to odours, such as would be found in a natural context.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.
authors.forename: Micheline
authors.initials: M
authors.lastname: Grillet
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.
authors.forename: Dario
authors.initials: D
authors.lastname: Campagner
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.
authors.forename: Rasmus
authors.initials: R
authors.lastname: Petersen
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: McCrohan
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK cobb@manchester.ac.uk.
authors.forename: Matthew
authors.identifier: 0000-0002-8258-4913
authors.initials: M
authors.lastname: Cobb
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000254:growth & development / Q000502:physiology*; D007814:Larva / Q000254:growth & development / Q000502:physiology; D055696:Olfactory Perception; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D013997:Time Factors
keywords: electrophysiology; olfaction; temporal coding