title: Coxiella burnetii Genotypes in Iberian Wildlife.
abstract: To investigate if Coxiella burnetii, the causative agent of Q fever, genotypes circulating in wildlife are associated with those infecting livestock and humans, multiple-locus variable number tandem-repeat analysis (MLVA-6-marker) was carried out over C. burnetii obtained from red deer (Cervus elaphus), Eurasian wild boar (Sus scrofa), European wild rabbit (Oryctolagus cuniculus), black rat (Rattus rattus), and wood mouse (Apodemus sylvaticus). MLVA typing was performed by using six variable loci in C. burnetii: Ms23, Ms24, Ms27, Ms28, Ms33, and Ms34. The C. burnetii cooperative database from MLVABank 5.0 was employed to compare genotypes found in this study with 344 isolates of diverse origin. Twenty-two genotypes from wildlife and two genotypes from domestic goats were identified. Some MLVA genotypes identified in wildlife or in farmed game clustered with genotypes of human Q fever clinical cases, supporting the idea that humans and wildlife share C. burnetii genotypes. The major part of genotypes identified in coexisting red deer and rabbits clustered according to their host of origin, suggesting host specificity for particular C. burnetii genotypes. These findings provide important insights to understand the epidemiology of C. burnetii at the wildlife-livestock-human interface.
authors.affiliation: Health & Biotechnology (SaBio) Group, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005, Ciudad Real, Spain. david.gonzalez@uclm.es.
authors.forename: David
authors.initials: D
authors.lastname: González-Barrio
authors.affiliation: Department of Medical Microbiology & Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
authors.forename: Ferry
authors.initials: F
authors.lastname: Hagen
authors.affiliation: Department of Medical Microbiology & Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
authors.forename: Jeroen J H C
authors.initials: JJ
authors.lastname: Tilburg
authors.affiliation: Health & Biotechnology (SaBio) Group, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005, Ciudad Real, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Ruiz-Fons
mesh_terms: D000818:Animals; D016997:Coxiella burnetii / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D003670:Deer / Q000382:microbiology*; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D058751:Livestock / Q000382:microbiology*; D051379:Mice; D058885:Multilocus Sequence Typing; D011778:Q Fever / Q000382:microbiology / Q000635:transmission* / Q000662:veterinary*; D011817:Rabbits; D051381:Rats; D013030:Spain; D034421:Sus scrofa / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: MLVA; Molecular ecology; Q fever; Zoonoses