title: High-Sensitivity Cardiac Troponin T: a Preanalytical Evaluation.
abstract: BACKGROUND
  Little is known about the effects of preanalytical conditions on high-sensitivity cardiac troponin T (hs-cTnT) concentrations.
  
  
  METHODS
  Variations of hs-cTnT concentrations were evaluated under the following preanalytical conditions: 1) serum vs. lithium-heparin (Li-Hep) plasma, with or without separator gel; 2) centrifugation time (15-minutes vs. 10-minutes) and speed (1467 to 3756 g); 3) stability in Li-Hep plasma at room temperature and +4 degrees C for 4 days and at -80 degrees C for up to 12 months, for three concentrations; 4) four freeze-thaw cycles at -20 degrees C and -80 degrees C, for three concentrations.
  
  
  RESULTS
  No significant changes were found regarding the type of blood collection tube, the centrifugation, and storage conditions. Minor decreases were observed after four freeze-thaw cycles at -20 degrees C (< 6.5%) and -80 degrees C (< 3.4%).
  
  
  CONCLUSIONS
  High-sensitivity cardiac troponin T may be considered as not impacted by usual preanalytical conditions, thus strengthening its reliability in laboratory practice and clinical research.
authors.forename: Moïse
authors.initials: M
authors.lastname: Michel
authors.forename: Fouzi
authors.initials: F
authors.lastname: Mestari
authors.forename: Rana
authors.initials: R
authors.lastname: Alkouri
authors.lastname: Atlan Grégory
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dever
authors.forename: Catherine
authors.initials: C
authors.lastname: Devilliers
authors.forename: Françoise
authors.initials: F
authors.lastname: Imbert-Bismut
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnefont-Rousselot
authors.forename: Denis
authors.initials: D
authors.lastname: Monneret
mesh_terms: D001793:Blood Preservation; D006801:Humans; D012680:Sensitivity and Specificity; D013696:Temperature; D020107:Troponin T / Q000097:blood*
chemical_list: D020107:Troponin T