title: ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses.
abstract: Measurements of humoral immune responses to West Nile virus (WNV) infection in mouse or other animal models are valuable components of basic laboratory investigations to assess immunogenicity of candidate vaccines or to evaluate seroconversion following challenge with WNV. Here, we outline the steps for screening or titrating of total antibodies by indirect enzyme linked immunosorbent assay (ELISA) as well as assessment of neutralizing antibody titers by immunofocus detection.
authors.affiliation: Department of Microbiology and Immunology, Sealy Center for Vaccine Development, Institute for Human Infections and Immunity, University of Texas Medical Branch, MRB, Route 0609, 301 University Blvd., Galveston, TX, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Friedrich
authors.affiliation: Department of Microbiology and Immunology, Sealy Center for Vaccine Development, Institute for Human Infections and Immunity, University of Texas Medical Branch, Keiller Building, Route 0609, 301 University Blvd., Galveston, TX, USA. dwbeasle@utmb.edu.
authors.forename: David W C
authors.initials: DW
authors.lastname: Beasley
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009500:Neutralization Tests; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology; D014901:West Nile Fever / Q000276:immunology*; D014902:West Nile virus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins
keywords: Antibody titration; ELISA; FRNT; Humoral immunity; Immunofocus; Neutralization; West Nile virus