title: Characterization of three positive regulators for tetramycin biosynthesis in Streptomyces ahygroscopicus.
abstract: Three putative regulatory genes, namely ttmRI, ttmRII and ttmRIII, which are present in the tetramycin (ttm) biosynthetic gene cluster, were found in Streptomyces ahygroscopicus Disruption of ttmRI, ttmRII or ttmRIII reduced tetramycin production, and their complementation restored production to varying degrees. Gene expression analysis of the wild-type (WT) and mutant strains through reverse transcriptase-polymerase chain reaction (RT-PCR) of the ttm gene cluster showed that the expression levels of most of the biosynthetic genes were reduced in ΔttmRI, ΔttmRII and ΔttmRIII Electrophoretic mobility shift assays demonstrated that TtmRI, TtmRII and TtmRIII bound the promoters of several genes in the ttm gene cluster. This study found that these three proteins are a group of positive regulators that activate the transcription of the ttm gene cluster in S. ahygroscopicus In addition, ΔttmRII had a reduced degree of grey pigmentation. Thus, TtmRII has a pleiotropic regulatory function in the tetramycin biosynthetic pathway and in development.
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cui
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Xianpu
authors.initials: X
authors.lastname: Ni
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Shoujia
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Zhenpeng
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Su
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China xiahz612@sina.com.
authors.forename: Huanzhang
authors.initials: H
authors.lastname: Xia
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D053898:Biosynthetic Pathways / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D018942:Macrolides / Q000378:metabolism*; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018942:Macrolides; C000593605:tetramycin
keywords: LAL family; regulator; regulatory network; streptomyces; tetramycin