title: EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae.
abstract: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD).
  
  
  
  PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd.
  
  
  
  EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium.
  
  
  
  Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.
authors.affiliation: Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-22184 Lund, Sweden.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Shadnezhad
authors.affiliation: Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-22184 Lund, Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Naegeli
authors.affiliation: Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-22184 Lund, Sweden.|Genovis AB, Scheelevägen 2, SE-220 07 Lund, Sweden.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sjögren
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Barbara
authors.initials: B
authors.lastname: Adamczyk
authors.affiliation: Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-22184 Lund, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Leo
authors.affiliation: Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-22184 Lund, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Allhorn
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Niclas G
authors.initials: NG
authors.lastname: Karlsson
authors.affiliation: Department of Biomedicine, Faculty of Health Sciences, Aarhus University, Aarhus, Denmark.
authors.forename: Anders
authors.initials: A
authors.lastname: Jensen
authors.affiliation: Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-22184 Lund, Sweden.
authors.forename: Mattias
authors.initials: M
authors.lastname: Collin
mesh_terms: D000118:Acetylglucosaminidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D013291:Streptococcus / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D004187:Disaccharides; D007074:Immunoglobulin G; D011134:Polysaccharides; C032438:chitobiose; D000118:Acetylglucosaminidase
keywords: Endo-β-N-acetylglucosaminidase; IgG; Streptococcus dysgalactiae; asparagine linked; chitinases; glycosidase