title: Downregulation of cell division cycle 25 homolog C reduces the radiosensitivity and proliferation activity of esophageal squamous cell carcinoma.
abstract: Radiation therapy is one of the most important methods of contemporary cancer treatment. Cells in the G2 and M phases are more sensitive to radiation therapy, and cell division cycle 25 homolog C (CDC25C) is essential in shifting the cell cycle between these two phases. In this study, the knockdown of CDC25C in human esophageal squamous carcinoma EC9706 cells was mediated by transfecting shRNA against human CDC25C-subcloning into pGV248. The levels of CDC25C mRNA and protein expression were assessed by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting, respectively. Moreover, cell proliferation and radiosensitivity were measured. Stable CDC25C-knockdown EC9706 cell lines were successfully established. Furthermore, the proliferation of both control and CDC25C-shRNA-EC9706 cells was inhibited after the cells were treated with increasing X-ray doses, and the proliferation of the control cells was affected more significantly (p<0.05). Moreover, cell colony formation assays allowed us to reach the same conclusion. Taken together, our experiments demonstrated that the knockdown of CDC25C can reduce both the radiotherapy sensitivity and the proliferation activity of EC9706 cells. Thus, CDC25C might be a potential biomarker for radiotherapy treatment.
authors.affiliation: Department of Radiotherapy, Anhui Cancer Hospital, Hefei, Anhui 230031, China. Electronic address: 976920812@qq.com.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Instiute of Hematology, Soochow University, Suzhou 215123, Jiangsu, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Dou
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China.
authors.forename: Shimiao
authors.initials: S
authors.lastname: Duan
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China.
authors.forename: Quanjing
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China. Electronic address: yycyyc1988@163.com.
authors.forename: Duojie
authors.initials: D
authors.lastname: Li
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011836:Radiation Tolerance / Q000235:genetics*; D014162:Transfection; D014410:Tumor Stem Cell Assay; D020687:cdc25 Phosphatases / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases
keywords: CDC25C; Esophageal cancer; RNA interference; Radiosensitivity