title: Electrochemical nanostructured ZnO biosensor for ultrasensitive detection of cardiac troponin-T.
abstract: Vertically oriented zinc oxide nanostructures based disposable diagnostic biosensor for detecting and quantifying levels of cardiac troponin-T from human serum has been developed.
  
  
  
  The biosensors were designed by integrating hydrothermally grown zinc oxide nanostructures on glass and printed circuit board platforms, resulting in the generation of high-density nanostructure arrays with nanotextured zinc oxide based electrodes. The size, density and surface terminations of the nanostructures were leveraged toward achieving surface confinement of the target cTnT molecules on to the nanostructures. A combination of AC and DC spectroscopy was used to characterize the biosensor response to cTnT.
  
  
  
  LOD of 0.1 pg/ml in human serum was achieved.
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors.forename: Nandhinee Radha
authors.initials: NR
authors.lastname: Shanmugam
authors.affiliation: Enlisense LLC, 1813 Audubon Pond Way, Allen, TX 75013, USA.
authors.forename: Sriram
authors.initials: S
authors.lastname: Muthukumar
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors.forename: Anjan Panneer
authors.initials: AP
authors.lastname: Selvam
authors.affiliation: Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
authors.forename: Shalini
authors.initials: S
authors.lastname: Prasad
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D020107:Troponin T / Q000097:blood*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D020107:Troponin T; D015034:Zinc Oxide
keywords: Mott–Schottky; cardiac troponin-T; electrochemical impedance spectroscopy; label-free biosensor; zinc oxide nano electrodes