title: Limited value of pro-inflammatory oxylipins and cytokines as circulating biomarkers in endometriosis - a targeted 'omics study.
abstract: Endometriosis is a common, complex gynecologic disorder characterized by the presence of endometrial-like tissues at extrauterine sites. Elevation in protein and lipid mediators of inflammation including oxylipins and cytokines within the peritoneum characterize the inflamed pelvic region and may contribute to the survival and growth of displaced endometrial tissues. The presence of a clinically silent but molecularly detectable systemic inflammation in endometriosis has been proposed. Thus, we examined serum oxylipin and immunomodulatory protein levels in 103 women undergoing laparoscopy to evaluate systematically any involvement in systemic pathophysiological inflammation in endometriosis. Oxylipin levels were similar between women with and without endometriosis. Stratification by menstrual phase or severity did not offer any difference. Women with ovarian endometriosis had significantly lower 12-HETE relative to peritoneal endometriosis (-50.7%). Serum oxylipin levels were not associated with pre-operative pain symptoms. Changes to immunomodulatory proteins were minimal, with IL-12(p70), IL-13 and VEGF significantly lower in mild endometriotic women compared to non-endometriotic women (-39%, -54% and -76% respectively). Verification using C-reactive protein as a non-specific marker of inflammation further showed similar levels between groups. The implications of our work suggest pro-inflammatory mediators in the classes studied may have potentially limited value as circulating biomarkers for endometriosis, suggesting of potentially tenuous systemic inflammation in endometriosis.
authors.affiliation: KK Research Centre, KK Women's and Children's Hospital, 100 Bukit Timah Road, 229899, Singapore.
authors.forename: Yie Hou
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602, Singapore.
authors.forename: Liang
authors.initials: L
authors.lastname: Cui
authors.affiliation: Saw Swee Hock School of Public Health, National University of Singapore, 12 Science Drive 2, #10-01, 117549, Singapore.
authors.forename: Jinling
authors.initials: J
authors.lastname: Fang
authors.affiliation: Division of Obstetrics &Gynaecology KK Women's and Children's Hospital, Singapore, 100 Bukit Timah Road, 229899, Singapore.
authors.forename: Bernard Su Min
authors.initials: BS
authors.lastname: Chern
authors.affiliation: Department of Reproductive Medicine, KK Women's and Children's Hospital, Singapore, 100 Bukit Timah Road, 229899, Singapore.
authors.forename: Heng Hao
authors.initials: HH
authors.lastname: Tan
authors.affiliation: KK Research Centre, KK Women's and Children's Hospital, 100 Bukit Timah Road, 229899, Singapore.|Department of Reproductive Medicine, KK Women's and Children's Hospital, Singapore, 100 Bukit Timah Road, 229899, Singapore.|Cancer and Stem Cell Biology, Duke-NUS Medical School, 8 College Road, 169857, Singapore.
authors.forename: Jerry K Y
authors.initials: JK
authors.lastname: Chan
mesh_terms: D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid; D016207:Cytokines / Q000097:blood*; D004715:Endometriosis / Q000175:diagnosis* / Q000473:pathology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000175:diagnosis / Q000473:pathology; D010535:Laparoscopy; D054883:Oxylipins / Q000097:blood*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D016207:Cytokines; D054883:Oxylipins