title: Anatomical and molecular consequences of Unilateral Naris Closure on two populations of olfactory sensory neurons expressing defined odorant receptors.
abstract: Mammalian olfactory sensory neurons (OSNs), the primary elements of the olfactory system, are located in the olfactory epithelium lining the nasal cavity. Exposed to the environment, their lifespan is short. Consequently, OSNs are regularly regenerated and several reports show that activity strongly modulates their development and regeneration: the peripheral olfactory system can adjust to the amount of stimulus through compensatory mechanisms. Unilateral naris occlusion (UNO) was frequently used to investigate this mechanism at the entire epithelium level. However, there is little data regarding the effects of UNO at the cellular level, especially on individual neuronal populations expressing a defined odorant receptor. Here, using UNO during the first three postnatal weeks, we analyzed the anatomical and molecular consequences of sensory deprivation in OSNs populations expressing the MOR23 and M71 receptors. The density of MOR23-expressing neurons is decreased in the closed side while UNO does not affect the density of M71-expressing neurons. Using Real Time qPCR on isolated neurons, we observed that UNO modulates the transcript levels for transduction pathway proteins (odorant receptors, CNGA2, PDE1c). The transcripts modulated by UNO will differ between populations depending on the receptor expressed. These results suggest that sensory deprivation will have different effects on different OSNs' populations. As a consequence, early experience will shape the functional properties of OSNs differently depending on the type of odorant receptor they express.
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Molinas
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.
authors.forename: Imad
authors.initials: I
authors.lastname: Aoudé
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Soubeyre
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.
authors.forename: Bassim
authors.initials: B
authors.lastname: Tazir
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Cadiou
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France. Electronic address: xavier.grosmaitre@u-bourgogne.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Grosmaitre
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D009296:Nasal Cavity; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D018035:Receptors, Odorant / Q000378:metabolism*; D012683:Sensory Deprivation / Q000502:physiology*
chemical_list: C502656:Olfr151 protein, mouse; C118713:Olfr16 protein, mouse; D018035:Receptors, Odorant
keywords: Anatomy; Expression levels; Main olfactory epithelium; Odorant receptor; Sensory deprivation; Transduction proteins