title: Circulation of clonal populations of fluoroquinolone-resistant CTX-M-15-producing Escherichia coli ST410 in humans and animals in Germany.
abstract: Multidrug-resistant Escherichia coli encoding CTX-M-type extended-spectrum β-lactamases (ESBLs) are isolated in increasing numbers from humans, companion animals and livestock, raising concern regarding the exchange and spread of isolates in these populations. In this study, whole-genome sequencing of CTX-M-15-producing E. coli isolates recently sampled from humans, companion animals, livestock and farm environments was performed. In total, 26 different sequence types (STs) were detected, of which ST410 was the most frequent and was the only ST present in all populations studied. Five clades (designated A-E) were detected within the ST410 isolates. In particular, isolates of clade B were present in all four populations and had core genomes that differed by less than 70 single nucleotide polymorphisms (SNPs). Isolates of clades B and C were also clonally marked, exhibiting identical chromosomal insertions of blaCTX-M-15 at distinct loci. These data provide strong evidence for the clonal dissemination of specific clades of CTX-M-15-producing E. coli ST410 in human and animal populations.
authors.affiliation: Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Giessen, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Falgenhauer
authors.affiliation: Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Giessen, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany.
authors.forename: Can
authors.initials: C
authors.lastname: Imirzalioglu
authors.affiliation: Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Giessen, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany.
authors.forename: Hiren
authors.initials: H
authors.lastname: Ghosh
authors.affiliation: Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Giessen, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany.
authors.forename: Konrad
authors.initials: K
authors.lastname: Gwozdzinski
authors.affiliation: Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Giessen, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Schmiedel
authors.affiliation: Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Giessen, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Gentil
authors.affiliation: Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Bauerfeind
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus Liebig University Giessen, Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: German Center for Infection Research (DZIF), Braunschweig, Germany; Institute for Medical Microbiology, Immunology, and Hygiene, University of Cologne, Cologne, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Seifert
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
authors.forename: Geovana Brenner
authors.initials: GB
authors.lastname: Michael
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.affiliation: Division of Nosocomial Pathogens and Antibiotic Resistances, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Pfeifer
authors.affiliation: Division of Nosocomial Pathogens and Antibiotic Resistances, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Werner
authors.affiliation: Division of Nosocomial Pathogens and Antibiotic Resistances, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Pietsch
authors.affiliation: Institute for Animal Hygiene and Environmental Health, Free University Berlin, Berlin, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Roesler
authors.affiliation: Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Berlin, Germany.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Guerra
authors.affiliation: Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Berlin, Germany.
authors.forename: Jennie
authors.initials: J
authors.lastname: Fischer
authors.affiliation: Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Berlin, Germany.
authors.forename: Hannah
authors.initials: H
authors.lastname: Sharp
authors.affiliation: Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Berlin, Germany.
authors.forename: Annemarie
authors.initials: A
authors.lastname: Käsbohrer
authors.affiliation: Institute of Bioinformatics and Systems Biology, Justus Liebig University Giessen, Giessen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.affiliation: Institute for Biometry, Epidemiology, and Information Processing, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Hille
authors.affiliation: Institute for Biometry, Epidemiology, and Information Processing, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Kreienbrock
authors.affiliation: Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner site Giessen-Marburg-Langen, Giessen, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany. Electronic address: Trinad.Chakraborty@mikrobio.med.uni-giessen.de.
authors.forename: Trinad
authors.initials: T
authors.lastname: Chakraborty
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004285:Dogs; D024901:Drug Resistance, Multiple, Bacterial; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary*; D024841:Fluoroquinolones / Q000494:pharmacology*; D014644:Genetic Variation; D016680:Genome, Bacterial; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D006801:Humans; D058751:Livestock; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases
keywords: Antimicrobial resistance; Epidemiology; Escherichia coli ST410