title: Linking N2O emission from biochar-amended composting process to the abundance of denitrify (nirK and nosZ) bacteria community.
abstract: Manure composting has been recognized as an important anthropogenic source of nitrous oxide (N2O) contributing to global warming. However, biochar effect on N2O emissions from manure composting is rarely evaluated, especially by linking it to abundance of denitrifying bacteria community. Results of this study indicated that biochar amendment significantly reduced N2O emissions from manure composting, primarily due to suppression of the nirK gene abundance of denitrifying bacteria. Pearson's correlation analysis showed a significant positive correlation between nirK abundance and N2O fluxes, while a negative correlation between nosZ density and N2O fluxes. Simultaneously, a linear correlation between nirK gene abundance minus nosZ gene abundance with N2O fluxes was also observed. In addition, a statistical model for estimating N2O emissions based on the bacterial denitrifying functional genes was developed and verified to adequately fit the observed emissions. Our results highlighted that biochar amendment would be an alternative strategy for mitigating N2O emissions during manure composting, and the information of related functional bacterial communities could be helpful for understanding the mechanism of N2O emissions.
authors.affiliation: Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.|Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Song
authors.affiliation: Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yaguo
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Shuwei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China. jwzou21@njau.edu.cn.|Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, China. jwzou21@njau.edu.cn.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Zou
keywords: Biochar; Denitrifying genes abundance; Manure composting; Modeling; Nitrous oxide