title: Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments.
abstract: Chilean mussel populations have been thought to be panmictic with limited genetic structure. Genotyping-by-sequencing approaches have enabled investigation of genomewide variation that may better distinguish populations that have evolved in different environments. We investigated neutral and adaptive genetic variation inMytilusfrom six locations in southern Chile with 1240 SNPs obtained with RAD-seq. Differentiation among locations with 891 neutral SNPs was low (FST= 0.005). Higher differentiation was obtained with a panel of 58 putative outlier SNPs (FST= 0.114) indicating the potential for local adaptation. This panel identified clusters of genetically related individuals and demonstrated that much of the differentiation (~92%) could be attributed to the three major regions and environments: extreme conditions in Patagonia, inner bay influenced by aquaculture (Reloncaví), and outer bay (Chiloé Island). Patagonia samples were most distinct, but additional analysis carried out excluding this collection also revealed adaptive divergence between inner and outer bay samples. The four locations within Reloncaví area were most similar with all panels of markers, likely due to similar environments, high gene flow by aquaculture practices, and low geographical distance. Our results and the SNP markers developed will be a powerful tool supporting management and programs of this harvested species.
authors.affiliation: Departamento de Producción Animal Facultad de Ciencias Agronómicas Universidad de Chile Avda Santa Rosa 11315, La Pintana 88 20808 Santiago Chile.
authors.forename: Cristián
authors.initials: C
authors.lastname: Araneda
authors.affiliation: Departamento de Ciencia de los Alimentos y Tecnología Química Facultad de Ciencias Químicas y Farmacéuticas Universidad de Chile Sergio Livingstone 1007, Independencia 8380492 Santiago Chile.
authors.forename: María Angélica
authors.initials: MA
authors.lastname: Larraín
authors.affiliation: Columbia River Inter-Tribal Fish Commission 3059-F National Fish Hatchery Road Hagerman ID 83332.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hecht
authors.affiliation: Columbia River Inter-Tribal Fish Commission 3059-F National Fish Hatchery Road Hagerman ID 83332.
authors.forename: Shawn
authors.initials: S
authors.lastname: Narum
keywords: FST outlier; RAD‐seq; SNP discovery; assignment tests; population structure