title: Measuring IL-1β Processing by Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor.
abstract: IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA have been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and in living macrophages either using a plate reader or a microscope.
authors.affiliation: Institut de Génomique Fonctionnelle, Labex ICST, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Université Montpellier, Montpellier, France.|Institut National de la Santé et de la Recherche Médicale Unité 1191, Montpellier, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Compan
authors.affiliation: Molecular Inflammation Group, Murcia Biomedical Research Institute (IMIB-Arrixaca), Hospital Virgen de la Arrixaca, Carretera Buenavista, s/n, Murcia, 30120, Spain. pablo.pelegrin@ffis.es.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pelegrín
mesh_terms: D000818:Animals; D060807:Bioluminescence Resonance Energy Transfer Techniques / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051379:Mice
chemical_list: D058847:Inflammasomes; D053583:Interleukin-1beta
keywords: BRET; Bioluminescence; IL-1β; Macrophage; Sensor