title: Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice.
abstract: Inflammatory cells, an integral component of tumor evolution, are present in Glioblastomas multiforme (GBM). To address the cellular basis and dynamics of the inflammatory microenvironment in GBM, we established an orthotopic syngenic model by grafting GL261-DsRed cells in immunocompetent transgenic LysM-EGFP//CD11c-EYFP reporter mice. We combined dynamic spectral two-photon imaging with multiparametric cytometry and multicolor immunostaining to characterize spatio-temporal distribution, morphology and activity of microglia and blood-derived infiltrating myeloid cells in live mice. Early stages of tumor development were dominated by microglial EYFP(+) cells invading the tumor, followed by massive recruitment of circulating LysM-EGFP(+) cells. Fluorescent invading cells were conventional XCR1(+) and monocyte-derived dendritic cells distributed in subpopulations of different maturation stages, located in different areas relative to the tumor core. The lethal stage of the disease was characterized by the progressive accumulation of EGFP(+)/EYFP(+) monocyte-derived dendritic cells. This local phenotypic regulation of monocyte subtypes marked a transition in the immune response.
authors.affiliation: Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France.|Services d'Anatomie Pathologique-Neuropathologique et de Pharmacie, Assistance Publique - Hopitaux de Marseille, Marseille, France.|Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France.|Centre de Recherche en Oncobiologie et Oncopharmacologie, INSERM UMR911 and Aix-Marseille Université, Marseille, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Ricard
authors.affiliation: Services d'Anatomie Pathologique-Neuropathologique et de Pharmacie, Assistance Publique - Hopitaux de Marseille, Marseille, France.|Centre de Recherche en Oncobiologie et Oncopharmacologie, INSERM UMR911 and Aix-Marseille Université, Marseille, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Tchoghandjian
authors.affiliation: Centre d'Immunophénomique, Aix-Marseille Université UM2, INSERM, US012, CNRS UMS3367, Marseille, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.affiliation: Centre d'Immunophénomique, Aix-Marseille Université UM2, INSERM, US012, CNRS UMS3367, Marseille, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Grenot
authors.affiliation: Services d'Anatomie Pathologique-Neuropathologique et de Pharmacie, Assistance Publique - Hopitaux de Marseille, Marseille, France.|Centre de Recherche en Oncobiologie et Oncopharmacologie, INSERM UMR911 and Aix-Marseille Université, Marseille, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Figarella-Branger
authors.affiliation: Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France.|Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Rougon
authors.affiliation: Centre d'Immunophénomique, Aix-Marseille Université UM2, INSERM, US012, CNRS UMS3367, Marseille, France.|Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université UM2, INSERM, U1104, CNRS UMR7280, Marseille, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Malissen
authors.affiliation: Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France.|Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Debarbieux
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005909:Glioblastoma / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D017628:Microglia / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D036641:Microscopy, Fluorescence, Multiphoton; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D064847:Multimodal Imaging / Q000379:methods*; D009368:Neoplasm Transplantation; D010641:Phenotype; D055815:Young Adult
chemical_list: D008164:Luminescent Proteins