title: Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation in Tetrahymena thermophila.
abstract: Although the role of cyclins in controlling nuclear division is well established, their function in ciliate meiosis remains unknown. In ciliates, the cyclin family has undergone massive expansion which suggests that diverse cell cycle systems exist, and this warrants further investigation. A screen for cyclins in the model ciliate Tetrahymena thermophila showed that there are 34 cyclins in this organism. Only 1 cyclin, Cyc17, contains the complete cyclin core and is specifically expressed during meiosis. Deletion of CYC17 led to meiotic arrest at the diakinesis-like metaphase I stage. Expression of genes involved in DNA metabolism and chromosome organization (chromatin remodeling and basic chromosomal structure) was repressed in cyc17 knockout matings. Further investigation suggested that Cyc17 is involved in regulating spindle pole attachment, and is thus essential for chromosome segregation at meiosis. These findings suggest a simple model in which chromosome segregation is influenced by Cyc17.
authors.affiliation: a Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan , People's Republic of China.|b University of Chinese Academy of Sciences , Beijing , People's Republic of China.
authors.forename: Guan-Xiong
authors.initials: GX
authors.lastname: Yan
authors.affiliation: c College of Life Sciences, Northwest Normal University , Lanzhou , People's Republic of China.
authors.forename: Huai
authors.initials: H
authors.lastname: Dang
authors.affiliation: a Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan , People's Republic of China.|d Department of Chromosome Biology and Max F. Perutz Laboratories , Center for Molecular Biology, University of Vienna , Vienna , Austria.
authors.forename: Miao
authors.initials: M
authors.lastname: Tian
authors.affiliation: a Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan , People's Republic of China.|b University of Chinese Academy of Sciences , Beijing , People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: d Department of Chromosome Biology and Max F. Perutz Laboratories , Center for Molecular Biology, University of Vienna , Vienna , Austria.
authors.forename: Anura
authors.initials: A
authors.lastname: Shodhan
authors.affiliation: c College of Life Sciences, Northwest Normal University , Lanzhou , People's Republic of China.
authors.forename: Ying-Zhi
authors.initials: YZ
authors.lastname: Ning
authors.affiliation: a Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan , People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.affiliation: a Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences , Wuhan , People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
mesh_terms: D000595:Amino Acid Sequence; D000705:Anaphase; D020090:Chromosome Segregation; D016213:Cyclins / Q000737:chemistry / Q000378:metabolism*; D008540:Meiosis; D008677:Metaphase; D009154:Mutation / Q000235:genetics; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D016808:Tetrahymena thermophila / Q000166:cytology* / Q000378:metabolism*
chemical_list: D016213:Cyclins; D015800:Protozoan Proteins
keywords: Tetrahymena thermophila; anaphase initiation; chromosome segregation; cyclin; meiosis