title: Long noncoding NONRATT021972 siRNA normalized abnormal sympathetic activity mediated by the upregulation of P2X7 receptor in superior cervical ganglia after myocardial ischemia.
abstract: Previous studies showed that the upregulation of the P2X7 receptor in cervical sympathetic ganglia was involved in myocardial ischemic (MI) injury. The dysregulated expression of long noncoding RNAs (lncRNAs) participates in the onset and progression of many pathological conditions. The aim of this study was to investigate the effects of a small interfering RNA (siRNA) against the NONRATT021972 lncRNA on the abnormal changes of cardiac function mediated by the up-regulation of the P2X7 receptor in the superior cervical ganglia (SCG) after myocardial ischemia. When the MI rats were treated with NONRATT021972 siRNA, their increased systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), low-frequency (LF) power, and LF/HF ratio were reduced to normal levels. However, the decreased high-frequency (HF) power was increased. GAP43 and tyrosine hydroxylase (TH) are markers of nerve sprouting and sympathetic nerve fibers, respectively. We found that the TH/GAP43 value was significantly increased in the MI group. However, it was reduced after the MI rats were treated with NONRATT021972 siRNA. The serum norepinephrine (NE) and epinephrine (EPI) concentrations were decreased in the MI rats that were treated with NONRATT021972 siRNA. Meanwhile, the increased P2X7 mRNA and protein levels and the increased p-ERK1/2 expression in the SCG were also reduced. NONRATT021972 siRNA treatment inhibited the P2X7 agonist BzATP-activated currents in HEK293 cells transfected with pEGFP-P2X7. Our findings suggest that NONRATT021972 siRNA could decrease the upregulation of the P2X7 receptor and improve the abnormal changes in cardiac function after myocardial ischemia.
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Tu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Qiulan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China. li.guilin@163.com.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, 330006, People's Republic of China. liangsd@hotmail.com.|Institute of Life Science of Nanchang University, Nanchang, 330006, People's Republic of China. liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D057809:HEK293 Cells; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D018408:Patch-Clamp Techniques; D062085:RNA, Long Noncoding / Q000378:metabolism* / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D058486:Receptors, Purinergic P2X7 / Q000096:biosynthesis*; D017783:Superior Cervical Ganglion / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000503:physiopathology; D015854:Up-Regulation
chemical_list: D062085:RNA, Long Noncoding; D058486:Receptors, Purinergic P2X7; C000611529:long non-coding RNA NONRATT021972, rat
keywords: Long noncoding RNA; Myocardial ischemia; P2X7 receptor; Superior cervical ganglia