title: Single-cell analysis reveals new subset markers of murine peritoneal macrophages and highlights macrophage dynamics upon Staphylococcus aureus peritonitis.
abstract: Resident macrophages play a central role in maintaining tissue homeostasis and immune surveillance. Here, we used single cell-based qPCR coupled with flow cytometry analysis to further define the phenotypes of large and small resident peritoneal macrophages (LPMs and SPMs, respectively) in mice. We demonstrated that the expression of Cxcl13, IfngR1, Fizz-1 and Mrc-1 clearly distinguished between LPMs and SPMs subsets. Using these markers, the dynamics of peritoneal macrophages in a Staphylococcus aureus-induced peritonitis model were analyzed. We found that S. aureus infection triggers a massive macrophage disappearance reaction in both subsets. Thereafter, inflammatory monocytes rapidly infiltrated the cavity and differentiated to replenish the SPMs. Although phenotypically indistinguishable from resident SPMs by flow cytometry, newly recruited SPMs had a different pattern of gene expression dominated by M2 markers combined with M1 associated features (inos expression). Interestingly, S. aureus elicited SPMs showed a robust expression of Cxcl13, suggesting that these cells may endorse the role of depleted LPMs and contribute to restoring peritoneal homeostasis. These data provide information on both resident and recruited macrophages dynamics upon S. aureus infection and demonstrate that single-cell phenotyping is a promising and highly valuable approach to unraveling macrophage diversity and plasticity.
authors.affiliation: Université de Toulouse, INP, ENVT, UMR1225, IHAP, F-31076 Toulouse, France INRA, UMR1225, IHAP, F-31076 Toulouse, France.
authors.forename: Solène
authors.initials: S
authors.lastname: Accarias
authors.affiliation: INRA, UAR1209 Département de Génétique Animale, INRA, Castanet-Tolosan, France GeT-PlaGe, Genotoul, INRA Auzeville F31326, Castanet-Tolosan, France.
authors.forename: Clémence
authors.initials: C
authors.lastname: Genthon
authors.affiliation: INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, Castanet-Tolosan, France CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, Castanet-Tolosan, France.
authors.forename: David
authors.initials: D
authors.lastname: Rengel
authors.affiliation: Université de Toulouse, INP, ENVT, UMR1225, IHAP, F-31076 Toulouse, France INRA, UMR1225, IHAP, F-31076 Toulouse, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Boullier
authors.affiliation: Université de Toulouse, INP, ENVT, UMR1225, IHAP, F-31076 Toulouse, France INRA, UMR1225, IHAP, F-31076 Toulouse, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Foucras
authors.affiliation: Université de Toulouse, INP, ENVT, UMR1225, IHAP, F-31076 Toulouse, France INRA, UMR1225, IHAP, F-31076 Toulouse, France g.tabouret@envt.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tabouret
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D000066670:Cell Plasticity; D002478:Cells, Cultured; D054382:Chemokine CXCL13 / Q000235:genetics / Q000378:metabolism; D005260:Female; D006706:Homeostasis; D006801:Humans; D007157:Immunologic Surveillance; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic; D017471:Receptors, Interferon / Q000235:genetics / Q000378:metabolism; D059010:Single-Cell Analysis / Q000379:methods*; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D000096743:Interferon gamma Receptor
chemical_list: D015415:Biomarkers; D054382:Chemokine CXCL13; D036341:Intercellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor; C581013:MRC1 protein, mouse; C413576:Retnla protein, mouse
keywords: CXCL13; Peritoneal macrophages; Staphylococcus aureus; homeostasis; single-cell analysis