title: Investigating the Swimming of Microbial Pathogens Using Digital Holography.
abstract: To understand much of the behaviour of microbial pathogens, it is necessary to image living cells, their interactions with each other and with host cells. Species such as Escherichia coli are difficult subjects to image: they are typically microscopic, colourless and transparent. Traditional cell visualisation techniques such as fluorescent tagging or phase-contrast microscopy give excellent information on cell behaviour in two dimensions, but no information about cells moving in three dimensions. We review the use of digital holographic microscopy for three-dimensional imaging at high speeds, and demonstrate its use for capturing the shape and swimming behaviour of three important model pathogens: E. coli, Plasmodium spp. and Leishmania spp.
authors.affiliation: Department of Physics, University of York, Heslington, York, YO10 5DD, England.
authors.forename: K L
authors.initials: KL
authors.lastname: Thornton
authors.affiliation: Department of Physics, University of York, Heslington, York, YO10 5DD, England.|Centre for Immunology and Infection, Department of Biology, University of York, Heslington, York, YO10 5DD, England.
authors.forename: R C
authors.initials: RC
authors.lastname: Findlay
authors.affiliation: Centre for Immunology and Infection, Department of Biology, University of York, Heslington, York, YO10 5DD, England.
authors.forename: P B
authors.initials: PB
authors.lastname: Walrad
authors.affiliation: Department of Physics, University of York, Heslington, York, YO10 5DD, England. laurence.wilson@york.ac.uk.
authors.forename: L G
authors.initials: LG
authors.lastname: Wilson
mesh_terms: D004926:Escherichia coli / Q000502:physiology*; D006696:Holography; D007091:Image Processing, Computer-Assisted; D007894:Leishmania mexicana / Q000502:physiology*; D008853:Microscopy; D009068:Movement; D061848:Optical Imaging / Q000379:methods*; D010962:Plasmodium berghei / Q000502:physiology*; D013997:Time Factors
keywords: Holography; Image analysis; Leishmania; Optical microscopy; Plasmodium