title: Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.
abstract: Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin RNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction, and data analysis.
authors.affiliation: Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarkbeeklaan 103, 1090, Jette, Brussel, Belgium. michael.mc.maes@vub.ac.be.
authors.forename: Michaël
authors.initials: M
authors.lastname: Maes
authors.affiliation: Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarkbeeklaan 103, 1090, Jette, Brussel, Belgium.
authors.forename: Joost
authors.initials: J
authors.lastname: Willebrords
authors.affiliation: Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarkbeeklaan 103, 1090, Jette, Brussel, Belgium.
authors.forename: Sara
authors.initials: S
authors.lastname: Crespo Yanguas
authors.affiliation: Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Cogliati
authors.affiliation: Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarkbeeklaan 103, 1090, Jette, Brussel, Belgium.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Vinken
mesh_terms: D000818:Animals; D017630:Connexins / Q000378:metabolism*; D017408:Guidelines as Topic; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D012313:RNA / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017630:Connexins; D012313:RNA
keywords: Connexins; Minimum Information for publication of Quantitative real-time PCR Experiments; RNA extraction; Reverse transcription