title: miR-30a can inhibit DNA replication by targeting RPA1 thus slowing cancer cell proliferation.
abstract: Cell proliferation was inhibited following forced over-expression of miR-30a in the ovary cancer cell line A2780DX5 and the gastric cancer cell line SGC7901R. Interestingly, miR-30a targets the DNA replication protein RPA1, hinders the replication of DNA and induces DNA fragmentation. Furthermore, ataxia telangiectasia mutated (ATM) and checkpoint kinase 2 (CHK2) were phosphorylated after DNA damage, which induced p53 expression, thus triggering the S-phase checkpoint, arresting cell cycle progression and ultimately initiating cancer cell apoptosis. Therefore, forced miR-30a over-expression in cancer cells can be a potential way to inhibit tumour development.
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China Life Sciences School of Nanjing University, Nanjing, JS 210093, China Biochemical Department of Purdue University, West Lafayette, IN 47906, U.S.A. sokuren@163.com.
authors.forename: Zhenyou
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Ni
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China Life Sciences School of Nanjing University, Nanjing, JS 210093, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Plant Protecting School of Nanjing Agricultural University, Nanjing, JS 210093, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Ma
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Shihan
authors.initials: S
authors.lastname: Qian
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Longhua
authors.initials: L
authors.lastname: Tang
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Tang
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Hailun
authors.initials: H
authors.lastname: Yao
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Xiongwen
authors.initials: X
authors.lastname: Lu
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Sun
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Qian
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Mao
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Xulin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Juping
authors.initials: J
authors.lastname: Zen
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Hanbing
authors.initials: H
authors.lastname: Wu
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Bao
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Shudan
authors.initials: S
authors.lastname: Lin
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Tumor Institute of Taizhou University, Taizhou, ZJ 318000, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zong
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology; D064447:Checkpoint Kinase 2 / Q000235:genetics / Q000378:metabolism; D020552:Comet Assay; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference / Q000502:physiology; D051716:Replication Protein A / Q000235:genetics / Q000378:metabolism*
chemical_list: C468783:H2AX protein, human; D006657:Histones; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C497167:RPA1 protein, human; D051716:Replication Protein A; D064447:Checkpoint Kinase 2; D064007:Ataxia Telangiectasia Mutated Proteins
keywords: DNA replication; RPA1; S-phase checkpoint; cell cycle arrest; miR-30a