title: Activities of proteasome and m-calpain are essential for Chikungunya virus replication.
abstract: Replication of many viruses is dependent on the ubiquitin proteasome system. The present study demonstrates that Chikungunya virus replication increases proteasome activity and induces unfolded protein response (UPR) in cultured cells. Further, it was seen that the virus replication was dependent on the activities of proteasomes and m-calpain. Proteasome inhibition induced accumulation of polyubiquitinated proteins and earlier visualization of UPR.
authors.affiliation: Nanobioscience Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411004, India. yakarpe@aripune.org.
authors.forename: Yogesh A
authors.initials: YA
authors.lastname: Karpe
authors.affiliation: Nanobioscience Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411004, India.
authors.forename: Kunal D
authors.initials: KD
authors.lastname: Pingale
authors.affiliation: Nanobioscience Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411004, India.
authors.forename: Gayatri D
authors.initials: GD
authors.lastname: Kanade
mesh_terms: D002154:Calpain / Q000378:metabolism*; D002460:Cell Line; D065632:Chikungunya Fever / Q000821:virology; D002646:Chikungunya virus / Q000235:genetics* / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics*
chemical_list: D025801:Ubiquitin; D002154:Calpain; C449000:m-calpain; D046988:Proteasome Endopeptidase Complex
keywords: Alphaviruses; Calpain; Chikungunya virus; Ubiquitin proteasome system (UPS); m-Calpain