title: Enhancement of immunogenic response and protection in model rats by CSTM nanoparticles anticaries DNA vaccine.
abstract: To construct anticaries DNA vaccine and evaluate its ability to elicit mucosal and systemic immune responses in rats.
  
  
  
  wapA fragment was cloned into pVAX1 plasmid to generate pVAX1-wapA. The pVAX1-wapA/trimethyl chitosan nanoparticles were prepared by complex coacervation method.
  
  
  
  Significantly higher specific IgG antibody titers were observed in rats immunized with nanoparticles compared with rats immunized with naked pVAX1-wapA. Anti-WapA IgA and IgG antibody levels after intranasal immunization were significantly higher than those following intramuscular delivery of nanoparticles or naked pVAX1-wapA. Furthermore, fewer enamel, slight dentin and dentin moderate lesions were observed in rats immunized with nanoparticles.
  
  
  
  The results implicate WapA as an excellent candidate for anticaries vaccine development and nanoparticles as an effective delivery system.
authors.affiliation: Changhai Hospital, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Hongjiao
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Lu
authors.affiliation: School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Xiang
authors.affiliation: School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Yanqiang
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D048271:Chitosan / Q000737:chemistry*; D003731:Dental Caries / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D007114:Immunization; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D053758:Nanoparticles; D010957:Plasmids; D017207:Rats, Sprague-Dawley; D013291:Streptococcus; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D048271:Chitosan
keywords: DNA vaccine; chitosan nanoparticles; dental caries; intranasal immunization; wall-associated protein A