title: Oxidative stress associated with pathological changes in the pancreas of cattle naturally infected by Eurytrema coelomaticum.
abstract: Although Eurytrema coelomaticum is considered a parasite with low pathogenicity, it may be associated with mortality and loss of productive performance in animals due to chronic pancreatitis. The aim of this study was to evaluate the occurrence of oxidative stress caused by E. coelomaticum in naturally infected cattle, correlating the biochemical findings with the parasite load and histopathological changes. For this study, blood and pancreas samples from 51 cattle were collected, and levels of the thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPP) and ferric reducing ability of plasma (FRAP) were measured in the serum and pancreas, and superoxide dismutase (SOD) activity was measured in total blood. Parasite burden was determined opening the pancreatic ducts, and then fragments of pancreas were collected and fixed in 10% buffered formalin and routinely processed for histopathology. From the 51 collected pancreas, 33 (63.5%) were parasitized. The average parasite burden per pancreas was 532 (12-2,578). TBARS and FRAP showed higher levels in serum and pancreas of infected animals (p<0.05), with a positive correlation between the histopathological changes and the number of parasites. SOD level in blood was 42% higher in parasitized group compared with control group (p<0.05), as well as AOPP in serum. Based on these results, we concluded that in natural infection by E. coelomaticum in cattle, oxidative stress occurs, characterized by the occurrence of protein oxidation, lipid peroxidation and activation of antioxidant system.
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Rod. SC 283, km 08, CP 58, Concórdia, SC 89703-720, Brazil.
authors.forename: Claiton I
authors.initials: CI
authors.lastname: Schwertz
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Rod. SC 283, km 08, CP 58, Concórdia, SC 89703-720, Brazil.
authors.forename: Mateus E
authors.initials: ME
authors.lastname: Gabriel
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Rod. SC 283, km 08, CP 58, Concórdia, SC 89703-720, Brazil.
authors.forename: Luan C
authors.initials: LC
authors.lastname: Henker
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil.
authors.forename: Guilherme do
authors.initials: Gd
authors.lastname: Carmo
authors.affiliation: Clinical and Toxicological Analysis Department, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil.
authors.forename: Naiara Dos S
authors.initials: Ndos S
authors.lastname: Guarda
authors.affiliation: Clinical and Toxicological Analysis Department, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil.
authors.forename: Rafael N
authors.initials: RN
authors.lastname: Moresco
authors.affiliation: College of Veterinary Medicine, Universidade Federal do Rio Grande do Sul (UFGRS), Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Machado
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil.
authors.forename: Vera M
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil.
authors.forename: Maria Rosa C
authors.initials: MR
authors.lastname: Schetinger
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Rod. SC 283, km 08, CP 58, Concórdia, SC 89703-720, Brazil.
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Stedille
authors.affiliation: Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), 8 Ciszewkiego, 02-786 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Baska
authors.affiliation: College of Veterinary Medicine, Universidade do Estado de Santa Catarina (UDESC), Av. Luís de Camões 2090, Lages, SC 88520-000, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Mattei
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil; Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Sete de Setembro 77D, Chapecó, SC 89806-152, Brazil.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: da Silva
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Rod. SC 283, km 08, CP 58, Concórdia, SC 89703-720, Brazil. Electronic address: ricardo.mendes@ifc-concordia.edu.br.
authors.forename: Ricardo E
authors.initials: RE
authors.lastname: Mendes
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology* / Q000473:pathology; D018509:DNA, Helminth / Q000235:genetics; D018384:Oxidative Stress / Q000502:physiology*; D010179:Pancreas / Q000469:parasitology / Q000473:pathology*; D010182:Pancreatic Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D014200:Trematoda / Q000145:classification*; D014201:Trematode Infections / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth
keywords: AOPP; Eurytrematosis; FRAP; SOD; TBARS