title: Production and immunogenicity of Actinobacillus pleuropneumoniae ApxIIA protein in transgenic rice callus.
abstract: Actinobacillus pleuropneumoniae is a major etiological agent that is responsible for swine pleuropneumonia, a highly contagious respiratory infection that causes severe economic losses in the swine production industry. ApxIIA is one of the virulence factors in A. pleuropneumoniae and has been considered as a candidate for developing a vaccine against the bacterial infection. A gene encoding an ApxIIA fragment (amino acids 439-801) was modified based on a plant-optimized codon and constructed into a plant expression vector under the control of a promoter and the 3' UTR of the rice amylase 3D gene. The plant expression vector was introduced into rice embryogenic callus (Oryza sativa L. cv. Dongjin) via particle bombardment-mediated transformation. The integration and transcription of the ApxIIA439-801gene were confirmed by using genomic DNA PCR amplification and Northern blot analysis, respectively. The synthesis of ApxIIA439-801antigen protein in transgenic rice callus was confirmed by western blot analysis. The concentration of antigen protein in lyophilized samples of transgenic rice callus was 250 μg/g. Immunizing mice with protein extracts from transgenic plants intranasally elicited secretory IgA. These results demonstrate the feasibility of using a transgenic plant to elicit immune responses against A. pleuropneumoniae.
authors.affiliation: Department of Molecular Biology, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Center for Jeongup Industry-Academy-Institute Cooperation, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors.forename: Tae-Geum
authors.initials: TG
authors.lastname: Kim
authors.affiliation: Department of Molecular Biology, Chonbuk National University, Jeonju, 561-756, Republic of Korea; Center for Jeongup Industry-Academy-Institute Cooperation, Chonbuk National University, Jeonju, 561-756, Republic of Korea; Department of Bioactive Material Sciences, Chonbuk National University, Jeonju, 561-756, Republic of Korea; Institute of Molecular Biology and Genetics, Chonbuk National University, Jeonju, 561-756, Republic of Korea. Electronic address: mskyang@jbnu.ac.kr.
authors.forename: Moon-Sik
authors.initials: MS
authors.lastname: Yang
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D005260:Female; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D059828:Plant Cells / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D007070:Immunoglobulin A
keywords: Actinobacillus pleuropneumoniae; ApxIIA; Codon optimization; Nasal route; Plant-based vaccine