title: Histone Modification Is Involved in Okadaic Acid (OA) Induced DNA Damage Response and G2-M Transition Arrest in Maize.
abstract: Histone modifications are involved in regulation of chromatin structure. To investigate the relationship between chromatin modification and cell cycle regulation during plant cell proliferation, Okadaic acid (OA), a specific inhibitor of serine/threonine protein phosphatase, was applied in this study. The results showed that OA caused the cell cycle arrest at preprophase, leading to seedling growth inhibition. Western blotting assay revealed that the spatial distribution of phosphorylation of Ser10 histone H3 tails (H3S10ph) signals was altered under OA treatment. Reactive oxygen species (ROS) was found to be at higher levels and TdT-mediated dUTP nick end labeling (TUNEL) assay displayed DNA breaks happened at the chromatin after treatment with OA, companied with an increase in the acetylation of histone H4 at lysine 5 (H4K5ac) level. From these observations, we speculated that the alteration of the spatial distribution of H3S10ph and the level of H4K5ac was involved in the procedure that OA induced DNA breaks and G2-M arrested by the accumulation of ROS, and that the histone H3S10ph and H4K5ac might facilitate DNA repair by their association with the chromatin decondensation.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Haoli
authors.initials: H
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Industrial Crops Institute of Hubei Academy of Agricultural Sciences, Hongshan District, Wuhan City, Hubei Province, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Wu
authors.affiliation: Industrial Crops Institute of Hubei Academy of Agricultural Sciences, Hongshan District, Wuhan City, Hubei Province, China.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D015153:Blotting, Western; D002455:Cell Division; D002843:Chromatin / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair; D016195:G2 Phase; D006657:Histones / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D008239:Lysine / Q000737:chemistry; D008938:Mitosis; D019319:Okadaic Acid / Q000494:pharmacology*; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013890:Thiourea / Q000737:chemistry; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000975:Antioxidants; D002843:Chromatin; D006657:Histones; D017382:Reactive Oxygen Species; D019319:Okadaic Acid; D013890:Thiourea; D008239:Lysine