title: A new fluorescence turn-on probe for biothiols based on photoinduced electron transfer and its application in living cells.
abstract: A new biothiol-selective fluorescent probe 1 based on photoinduced electron transfer (PET) mechanism was designed and synthesized. The UV-Vis absorption and fluorescent emission properties of probe 1 towards various analytes were studied in detail. The probe exhibited a large stokes shift (~200nm) after reacted with biothiols and could selectively detect cysteine (Cys) in dimethyl sulfoxide (DMSO)/H2O solution (9:1, v/v, 10mM phosphate buffer saline, pH3.5) over glutathione (GSH), homocysteine (Hcy) and other analytes with a detection limit of 0.117μM. In addition, probe 1 responded well to GSH, Hcy and Cys in the same above solution with pH5.5 and got the detection limits of 0.151μM, 0.128μM and 0.037μM, respectively. Probe 1 was of very low cytotoxicity and successfully applied for imaging of thiols in living cells.
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Jianjian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address: zhanghx@lzu.edu.cn.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D003545:Cysteine / Q000032:analysis*; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D006367:HeLa Cells; D006710:Homocysteine / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D061848:Optical Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D006710:Homocysteine; D005978:Glutathione; D003545:Cysteine
keywords: Biothiols; Fluorescent probe; Imaging; Photoinduced electron transfer