title: Chemometric analysis of attenuated total reflectance infrared spectra of Proteus mirabilis strains with defined structures of LPS.
abstract: Proteus spp. strains are some of the most important pathogens associated with complicated urinary tract infections and bacteremia affecting patients with immunodeficiency and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods have been developed for this pathogen. However, these methods are labor intensive and time consuming. We evaluated a new method of differentiation between strains. A collection of Proteus spp. strains was analyzed by attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectroscopy in the mid-infrared region. ATR FT-IR spectroscopy used in conjunction with a diamond ATR accessory directly produced the biochemical profile of the surface chemistry of bacteria. We conclude that a combination of ATR FT-IR spectroscopy and mathematical modeling provides a fast and reliable alternative for discrimination between Proteus isolates, contributing to epidemiological research.
authors.affiliation: Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland paulina.zarnowiec@gmail.com.
authors.forename: Paulina
authors.initials: P
authors.lastname: Zarnowiec
authors.affiliation: Computer Science and Communications Research Unit, University of Luxembourg, Luxembourg.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Mizera
authors.affiliation: Institute of Mathematics, Jan Kochanowski University, Kielce, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chrapek
authors.affiliation: Institute of Chemistry, Jan Kochanowski University, Kielce, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Urbaniak
authors.affiliation: Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland.
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Kaca
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000175:diagnosis*; D005240:Feasibility Studies; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D008962:Models, Theoretical; D011044:Poland / Q000453:epidemiology; D011512:Proteus Infections / Q000175:diagnosis* / Q000453:epidemiology; D011513:Proteus mirabilis / Q000145:classification / Q000378:metabolism*; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis*
chemical_list: D008070:Lipopolysaccharides
keywords: Differentiation index; Kohonen network; Proteus spp; hierarchical cluster analysis; infrared spectroscopy; lipopolysaccharide; principal component analysis; random forest