title: Antiviral Activity of Oroxylin A against Coxsackievirus B3 Alleviates Virus-Induced Acute Pancreatic Damage in Mice.
abstract: The flavonoids mosloflavone, oroxylin A, and norwogonin, which were purified from Scutellaria baicalensis Georgi, significantly protected Vero cells against Coxsackievirus B3 (CVB3)-induced cell death. To investigate the in vivo antiviral activity of oroxylin A, we intraperitoneally inoculated CVB3 into 4-week-old BALB/c mice. Body weights and blood glucose levels of the mice were decreased after CVB3 infection, and these changes were attenuated by the administration of oroxylin A. Importantly, treatment of mice with oroxylin A reduced viral titers in the pancreas and decreased the serum levels of the inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α. Additionally, the administration of oroxylin A mitigated the histological pancreatic lesions and apoptotic cell death induced by CVB3 infection and increased the levels of phospho-eIF2α in infected pancreata. The results suggest that oroxylin A may represent a potent antiviral agent against CVB3 infection.
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Bo-Eun
authors.initials: BE
authors.lastname: Kwon
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Jae-Hyoung
authors.initials: JH
authors.lastname: Song
authors.affiliation: Agency for Korea National Food Cluster (AnFC), Iksan, Korea.
authors.forename: Hyuk-Hwan
authors.initials: HH
authors.lastname: Song
authors.affiliation: Department of Biomedical Laboratory Science, Yonsei University, Wonju, 220-710, Republic of Korea.
authors.forename: Ju Won
authors.initials: JW
authors.lastname: Kang
authors.affiliation: Department of Biomedical Laboratory Science, Yonsei University, Wonju, 220-710, Republic of Korea.
authors.forename: Sam Noh
authors.initials: SN
authors.lastname: Hwang
authors.affiliation: Department of Biomedical Laboratory Science, Yonsei University, Wonju, 220-710, Republic of Korea.
authors.forename: Ki-Jong
authors.initials: KJ
authors.lastname: Rhee
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Aeri
authors.initials: A
authors.lastname: Shim
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Inje University, Gimhae, 621-749, South Korea.
authors.forename: Yeon-Jeong
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Ajou University, Suwon, 443-749, South Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Jeon
authors.affiliation: College of Pharmacy, Ajou University, Suwon, 443-749, South Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Chang
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience & Biotechnology, Cheongju, South Korea.
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
authors.affiliation: Anticancer Agent Research Center, Korea Research Institute of Bioscience & Biotechnology, Cheongju, South Korea.
authors.forename: Sungchan
authors.initials: S
authors.lastname: Cho
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Ko
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis; D002470:Cell Survival; D002522:Chlorocebus aethiops; D003384:Coxsackievirus Infections / Q000188:drug therapy*; D016207:Cytokines / Q000378:metabolism; D004770:Enterovirus / Q000187:drug effects; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D005260:Female; D047309:Flavones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010182:Pancreatic Diseases / Q000188:drug therapy* / Q000821:virology*; D010766:Phosphorylation; D031363:Scutellaria; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D016207:Cytokines; D015852:Eukaryotic Initiation Factor-2; D047309:Flavones; D005419:Flavonoids; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C035064:5-hydroxy-7,8-dimethoxyflavone; C055505:norwogonin; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one