title: Paenibacillusliaoningensis sp. nov., isolated from soil.
abstract: A novel bacterial strain, designated as LNUB461T, was isolated from soil sample taken from the countryside of Shenyang, Liaoning Province, China. The isolate was a Gram-stain-positive, aerobiotic, motile, endospore-forming and rod-shaped bacterium. The organism grew optimally at 30-33 °C, pH 6.5-7.0 and in the absence of NaCl. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence revealed high sequence similarity with Paenibacillus algorifonticola XJ259T (98.5 %), Paenibacillus xinjiangensis B538T (96.8 %), Paenibacillus glycanilyticus DS-1T (96.1 %) and Paenibacillus lupini RLAHU15T (96.1 %). The predominant cellular fatty acid and the only menaquinone were anteiso-C15:0 and MK-7, respectively. The main polar lipids of LNUB461T included phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and two unknown amino phospholipids (APL), and the cell-wall peptidoglycan was meso-diaminopimelic acid (A1γ). The DNA G+C content of LNUB461T was 49.1 mol%. The DNA-DNA hybridization value between LNUB461T and the most closely related species (P. algorifonticola) was 41.8 %. On the basis of these data, LNUB461T was classified as representing a novel species of the genus Paenibacillus, for which the name Paenibacillus liaoningensis sp. nov was proposed. The type strain is LNUB461T (=JCM 30712T=CGMCC 1.15101T).
authors.affiliation: School of Life Science, Liaoning University, Shenyang 110036, PR China.|Liaoning Engineering Laboratory for Molecular Simulation and Designing of Drug Molecules, Shenyang 110036, PR China.|Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning, Shenyang 110036, PR China.
authors.forename: Hai-Xin
authors.initials: HX
authors.lastname: Ai
authors.affiliation: School of Basic Medical Science, Shenyang Medical College, Shenyang 110034, PR China.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Che
authors.affiliation: School of Pharmacy, Liaoning University, Shenyang 110036, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Life Science, Liaoning University, Shenyang 110036, PR China.|Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning, Shenyang 110036, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Science, Liaoning University, Shenyang 110036, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.affiliation: School of Life Science, Liaoning University, Shenyang 110036, PR China.
authors.forename: Yan-Ni
authors.initials: YN
authors.lastname: Tan
authors.affiliation: School of Life Science, Liaoning University, Shenyang 110036, PR China.
authors.forename: Alan K
authors.initials: AK
authors.lastname: Chang
authors.affiliation: Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning, Shenyang 110036, PR China.|Liaoning Engineering Laboratory for Molecular Simulation and Designing of Drug Molecules, Shenyang 110036, PR China.|School of Life Science, Liaoning University, Shenyang 110036, PR China.
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Liu
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7