title: Glutathione S-transferase A1 - a sensitive marker of alcoholic injury on primary hepatocytes.
abstract: The primary hepatocytes were extracted and purified from mice through improved Seglen two-step perfusion method. Ethanol-induced injury hepatocytes model in mice was used to investigate the importance of glutathione S-transferase A1 (GSTA1) in hepatocytes injury by comparison with other indicators, such as alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione and superoxide dismutase. The release of GSTA1 was demonstrated to be an earlier and more sensitive indicator of hepatocytes injury than other indicators. Significant increases in GSTA1 were detected at 2 h after ethanol exposure, while other indicators were undetected at this time. A markedly difference in other indicators were observed at 6 and 8 h. The release of GSTA1 was significantly increased at a concentration of 50 mmol/L ethanol, the lowest exposure concentration than that in other indicators. In contrast, other indicators release was not statistically significant until concentrations of 75 mmol/L and 100 mmol/L ethanol. These results suggest that GSTA1 can be detected at the early stage of low concentration ethanol exposure and that GSTA1 is more sensitive and reliable marker in ethanol-induced hepatic injury.
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: Y-C
authors.initials: YC
authors.lastname: Chang
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: F-P
authors.initials: FP
authors.lastname: Liu
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: M-M
authors.initials: MM
authors.lastname: Li
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.affiliation: 2 Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
authors.forename: C-W
authors.initials: CW
authors.lastname: Li
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: C-X
authors.initials: CX
authors.lastname: Shi
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: J-S
authors.initials: JS
authors.lastname: He
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: Y-X
authors.initials: YX
authors.lastname: Lin
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: C-W
authors.initials: CW
authors.lastname: Zhao
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Han
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: Y-L
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
authors.forename: D-N
authors.initials: DN
authors.lastname: Wang
authors.affiliation: 2 Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
authors.forename: J-L
authors.initials: JL
authors.lastname: Liu
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002478:Cells, Cultured; D000431:Ethanol / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D000431:Ethanol; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione
keywords: GSTA1; alcoholic injury; marker; primary hepatocytes