title: Implication of the estrogen receptors GPER, ESR1, ESR2 in post-testicular maturations of equine spermatozoa.
abstract: Estrogen receptors ESR1, ESR2 and GPER are present on mature ejaculated horse spermatozoa, suggesting these cells as putative targets for estrogens. Indeed, spermatozoa are exposed to high level of estrogens during the transit in the male and female genital tracts but their roles are not investigated. So, we evaluated in vitro the role of 17β-estradiol during post-testicular maturations: regulation of motility, capacitation and acrosome reaction. Moreover according to the pseudo-seasonal breeder status of the stallion, we analyzed the putative seasonal variations in the presence of ESRs in spermatozoa. We showed that ESRs are more present on stallion sperm during the breeding season. We showed that capacitation and acrosome reaction are independent of estradiol action in horse. Estradiol can weakly modulate the motility and this effect is strictly associated with GPER and not with ESR1 and ESR2. The subcellular localization of GPER in the neck on stallion sperm is coherent with this effect. It seems that estrogens are not major regulators of sperm maturations associated to mare genital tract, so they could act during the epididymal maturations.
authors.affiliation: Normandie Univ, France; UNICAEN, EA2608, OeReCa, F-14032 Caen, France; USC-INRA 1377, F-14032 Caen, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Gautier
authors.affiliation: Jumenterie du Pin, Institut Français du Cheval et de l'Equitation, 61310 Exmes, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Barrier-Battut
authors.affiliation: Normandie Univ, France; UNICAEN, EA2608, OeReCa, F-14032 Caen, France; USC-INRA 1377, F-14032 Caen, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Guénon
authors.affiliation: Normandie Univ, France; UNICAEN, CMABIO, F-14032 Caen, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Goux
authors.affiliation: Normandie Univ, France; UNICAEN, EA2608, OeReCa, F-14032 Caen, France; USC-INRA 1377, F-14032 Caen, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Delalande
authors.affiliation: Normandie Univ, France; UNICAEN, EA2608, OeReCa, F-14032 Caen, France; USC-INRA 1377, F-14032 Caen, France. Electronic address: helene.bouraima@unicaen.fr.
authors.forename: Hélène
authors.initials: H
authors.lastname: Bouraïma-Lelong
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000378:metabolism / Q000502:physiology*; D047629:Estrogen Receptor beta / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006736:Horses / Q000235:genetics / Q000502:physiology*; D008297:Male; D011960:Receptors, Estrogen / Q000378:metabolism / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism / Q000502:physiology*; D013075:Sperm Capacitation / Q000187:drug effects; D013079:Sperm Maturation / Q000187:drug effects / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects; D013083:Sperm Transport / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D004958:Estradiol
keywords: Capacitation; Estradiol; GPER; Sperm motility; Spermatozoa; Stallion