title: Association of long noncoding RNA and c-JUN expression in hepatocellular carcinoma.
abstract: BACKGROUND
  Long noncoding RNAs(lncRNAs) have emerged as key elements in modulating gene expression in different biological contexts.
  
  
  METHODS
  We used quantitative real-time PCR (Qpcr) to evaluate the expression of lncRNA-UCA1 and C-JUN in serum of 70 patients with hepatocellular carcinoma (HCC), 32 patients chronic hepatitis C (CHC) and 38 healthy subjects and their correlation with different clinicopathological factors.
  
  
  RESULTS
  The expression of lncRNA-UCA1 and C-JUN was positive in 91.4%HCC patients with strong discriminating power between HCC and healthy subjects and CHC patients as well. The median follow up period was 29 months. The survival analysis showed that both lncRNA-UCA1 and C-JUN were independent prognostic factors. Of note, we identified C-JUN expression changes consistent with the lncRNA-UCA1 target regulation.
  
  
  CONCLUSIONS
  This information sheds light on the possible role of lncRNA-UCA1 and C-JUN mRNA as promising diagnostic and prognostic markers as well as potential therapeutic targets in HCC.
authors.affiliation: a General and Plastic Surgery Department , Military Medical Academy , Cairo , Egypt.
authors.forename: Ahmed H F
authors.initials: AH
authors.lastname: El-Tawdi
authors.affiliation: b Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt.
authors.forename: Marwa
authors.initials: M
authors.lastname: Matboli
authors.affiliation: c Hepatology and Gastroenterology Unit, Internal Medicine Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt.
authors.forename: Sarah
authors.initials: S
authors.lastname: El-Nakeep
authors.affiliation: d Armed Forces College of Medicine , Cairo , Egypt.
authors.forename: Ahmed E M
authors.initials: AE
authors.lastname: Azazy
authors.affiliation: e Clinical Oncology Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt.
authors.forename: Omar
authors.initials: O
authors.lastname: Abdel-Rahman
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D016755:Proto-Oncogene Proteins c-jun / Q000097:blood / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; D016755:Proto-Oncogene Proteins c-jun; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C515159:UCA1 RNA, human
keywords: C-JUN; bioinformatics; biomarkers; hepatocellular carcinoma; lncRNA-UCA1; prognosis