title: Lactate administration increases mRNA expression of PGC-1α and UCP3 in mouse skeletal muscle.
abstract: To examine the potential role of lactate as a signalling molecule in skeletal muscle, we performed global gene expression analysis of the mouse gastrocnemius muscle, 3 h after lactate administration using the Affymetrix GeneChip system (Affymetrix, Santa Clara, Calif., USA). Among the top 15 genes with the largest fold change, increased expression of Ppargc1a, Pdk4, and Ucp3 was confirmed using real-time quantitative polymerase chain reaction. Our findings suggest that lactate serves as a signal for upregulating genes related to mitochondrial function.
authors.affiliation: a Department of Sports Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kitaoka
authors.affiliation: b Graduate School of Comprehensive Human Science, University of Tsukuba, Tsukuba, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Takeda
authors.affiliation: a Department of Sports Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tamura
authors.affiliation: a Department of Sports Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Hatta
mesh_terms: D000818:Animals; D019344:Lactic Acid / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000071247:Uncoupling Protein 3 / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C000631439:Pdk4 protein, mouse; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D012333:RNA, Messenger; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3; D019344:Lactic Acid; D017346:Protein Serine-Threonine Kinases
keywords: PDK4; PGC-1α; UCP3; lactate; muscle squelettique; skeletal muscle