title: Snapshot on a Pilot Metagenomic Study for the Appraisal of Gut Microbial Diversity in Mice, Cat, and Man.
abstract: Gut microbiota plays a key role in the maintenance of homeostasis and host physiology, comprising development, metabolism, and immunity. Profiling the composition and the gastrointestinal microbiome with a reliable methodology is of substantial interest to yield new insights into the pathogenesis of many diseases as well as defining new prophylactic and therapeutic interventions. Here, we briefly present our methodology applied to fecal samples from mice and then further extended to the samples from a cat and a single human subject at 4 different time points as examples to illustrate the methodological strengths. Both interindividual and time-related variations are demonstrated and discussed.
authors.affiliation: Université Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019, UMR 8204, Centre d'Infection et d'Immunité de Lille, 59000 Lille, France.
authors.forename: Coline
authors.initials: C
authors.lastname: Plé
authors.affiliation: Genoscreen, Service of Research, Development and Innovation in Health and Environment, 1 rue du Pr Calmette, BP 245, 59019 Lille, France.
authors.forename: Louise-Eva
authors.initials: LE
authors.lastname: Vandenborght
authors.affiliation: Genoscreen, Service of Research, Development and Innovation in Health and Environment, 1 rue du Pr Calmette, BP 245, 59019 Lille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Adele-Dit-Renseville
authors.affiliation: Université Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019, UMR 8204, Centre d'Infection et d'Immunité de Lille, 59000 Lille, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Breton
authors.affiliation: Université Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019, UMR 8204, Centre d'Infection et d'Immunité de Lille, 59000 Lille, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Daniel
authors.affiliation: Genoscreen, Service of Research, Development and Innovation in Health and Environment, 1 rue du Pr Calmette, BP 245, 59019 Lille, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Ferreira
authors.affiliation: Université Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019, UMR 8204, Centre d'Infection et d'Immunité de Lille, 59000 Lille, France.
authors.forename: Foligné
authors.initials: F
authors.lastname: Benoît