title: [Genomic characteristics of 2 strains of influenza A(H9N2)virus isolated from human infection cases in Anhui province].
abstract: OBJECTIVE
  To understand genomic characteristics of 2 strains of influenza A(H9N2)virus isolated from human infection cases in Anhui province in 2015.
  
  
  METHODS
  Two human infection with H9N2 virus were confirmed by national influenza surveillance laboratory network in Anhui through viral isolation in April and September, 2015, respectively. The full genomic sequences of the two viral isolates were analyzed in this study by using molecular bioinformatics software Mega 6.0.
  
  
  RESULTS
  Human infection with H9N2 virus was first reported in Anhui province. The analysis of genomic sequence showed that the HA and NA genes of the two H9N2 isolates belonged to A/Chicken/Shanghai/F/98(H9N2)-like lineage, and shared high identity with H9N2 virus circulating in poultry in 2013. The PB2 and MP genes belonged to the A/quail/Hong Kong/G1/97-like lineage, and shared high homology with H7N9, H10N8 or H6N2 viruses. The amino acid sequence alignment results showed that several mutations for human infection tropism presented in the two virus strains, including Q226L, H183N and E190T in HA; S31N in M2; 63-65 deletion in NA. In addition, the H9N2 influenza virus strains possessed the PSRSSR\GL motif in HA. Meanwhile several human-like signatures, including PA-100A, PA-356R and PA-409N were also found in the two virus strains.
  
  
  CONCLUSIONS
  The H9N2 viruses isolated from human infection cases in Anhui province belonged to a reassortant virus originated from different lineage H9N2 avian influenza virus. The virus has possessed several human susceptibility locus.
authors.affiliation: School of Public Health, Anhui Medical University, Hefei 230601, China; Department of Microbiology Laboratory, Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Microbiology Laboratory, Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.
authors.forename: L P
authors.initials: LP
authors.lastname: Liu
authors.affiliation: Department of Acute Infectious Diseases, Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.
authors.forename: S
authors.initials: S
authors.lastname: Hou
authors.affiliation: Department of Acute Infectious Diseases, Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.
authors.forename: L
authors.initials: L
authors.lastname: Gong
authors.affiliation: Department of Acute Infectious Diseases, Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.
authors.forename: J B
authors.initials: JB
authors.lastname: Wu
authors.affiliation: Department of Microbiology Laboratory, Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.
authors.forename: W F
authors.initials: WF
authors.lastname: Hu
authors.affiliation: School of Public Health, Anhui Medical University, Hefei 230601, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002681:China / Q000453:epidemiology; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype; D053127:Influenza A Virus, H9N2 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D007251:Influenza, Human / Q000175:diagnosis / Q000208:ethnology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011200:Poultry; D011201:Poultry Diseases / Q000821:virology*; D016865:Reassortant Viruses; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins