title: Dataset of gene cloning and gel filtration chromatography of R-est6.
abstract: The data presented in this article are connected to the research article entitled "Expression, purification and biochemical characterization of a family 6 carboxylesterase from Methylococcus capsulatus (bath)" (Soni et al., 2016 [1]). The family 6 carboxylesterases are the smallest and display broad substrate specificity. The 1 kb gene encoding, a family 6 carboxylesterase - R-est6, was amplified from the genome of M. capsulatus (bath strain), and showed in the agarose gel. The corresponding purified protein, after overexpression in Escherichia coli, was biochemically studied in the research article (Soni et al., 2016 [1]). R-est6 has hydrophobic patches on the surface so, it is expected to show oligimeric forms. Here, we have confirmed the presence of oligomers by gel filtration chromatography data and the proteins belonging to the different peaks are shown on a SDS-PAGE.
authors.affiliation: DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Surabhi
authors.initials: S
authors.lastname: Soni
authors.affiliation: DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Annamma A
authors.initials: AA
authors.lastname: Odaneth
authors.affiliation: DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (East), Mumbai 400019, Maharashtra, India; Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Lali
authors.affiliation: DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (East), Mumbai 400019, Maharashtra, India.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Chandrayan