title: Potato wound-healing tissues: A rich source of natural antioxidant molecules with potential for food preservation.
abstract: The need for safe, effective preservatives is a prominent issue in the food and drug industries, reflecting demand for natural alternatives to synthetic chemicals viewed as harmful to consumers and the environment. Thus, this study determined the identities and scavenging capacities of antioxidant metabolites produced as a response to potato tuber wounding, using activity-guided fractionation of polar extracts from a Yukon Gold cultivar that had previously exhibited exceptionally high radical-scavenging activity. Activity-guided fractionation using the ABTS(+) radical scavenging assay and LC-MS with TOF-MS for compositional analysis of the most potent antioxidant fractions yielded identification of nine constituents: coumaroylputrescine; feruloylquinic acid; isoferuloylputrescine; ferulic acid; 22,25-dimethoxy-3-[[2,3,4-tri-O-methyl-6-O-(2,3,4,6-tetra-O-methyl-β-d-glucopyranosyl)-β-d-glucopyranosyl]oxy]-(3β)-lanost-9(11)-en-24-one; 4-(2Z)-2-decen-1-yl-5-[1-(4-hydroxyphenyl)decyl]-1,2-benzenediol; 8-[(2E)-3,7-dimethyl-2,6-octadien-1-yl]-5-hydroxy-2,8-dimethyl-6-(3-methyl-2-buten-1-yl)-2H-1-benzopyran-4,7(3H,8H)-dione; 3-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]-20-[(6-O-β-d-xylopyranosyl-β-d-glucopyranosyl)oxy]-dammar-24-en-19-al; (3β)-28-oxo-28-(phenylmethoxy)oleanan-3-yl 2-O-β-d-galactopyranosyl-3-O-(phenylmethyl)-, butyl ester β-d-glucopyranosiduronic acid. A positive correlation was observed between the scavenging activities and the polarities of the active fractions. The antioxidant capacities of the fractions were also characterised by monitoring the activity throughout a 45-minute assay period.
authors.affiliation: Department of Chemistry and Biochemistry, The City College of New York, City University of New York Graduate Center Ph.D. Programs in Biochemistry and Chemistry, and CUNY Institute for Macromolecular Assemblies, New York, NY 10031, USA.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Dastmalchi
authors.affiliation: Department of Chemistry and Biochemistry, The City College of New York, City University of New York Graduate Center Ph.D. Programs in Biochemistry and Chemistry, and CUNY Institute for Macromolecular Assemblies, New York, NY 10031, USA.
authors.forename: Isabel
authors.initials: I
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biochemistry, The City College of New York, City University of New York Graduate Center Ph.D. Programs in Biochemistry and Chemistry, and CUNY Institute for Macromolecular Assemblies, New York, NY 10031, USA. Electronic address: rstark@ccny.cuny.edu.
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Stark
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives; D016166:Free Radical Scavengers; D035281:Plant Tubers / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry* / Q000502:physiology*
chemical_list: D000975:Antioxidants; D005520:Food Preservatives; D016166:Free Radical Scavengers
keywords: ABTS(+) scavenging activity; Antioxidants; LC–MS; Potato; Solanum tuberosum; TOF-MS; Wound periderm