title: Decreased proportions of CD4 + IL17+/CD4 + CD25 + CD127- and CD4 + IL17+/CD4 + CD25 + CD127 - FoxP3+ T cells in children with autoimmune thyroid diseases (.).
abstract: Until now, altered balance of Th1 and Th2 immune cells has been postulated to play an important role in the pathogenesis of autoimmune thyroid diseases (AITD). However, recent studies on thyroid diseases have suggested a new role for Th17 cells that have been classified as a new lineage, distinct from Th1, Th2 and Treg cells. Despite wide interest, the role of Th17 cells in the pathogenesis of inflammatory and autoimmune diseases is still debated. The aim of the study was to estimate the proportions of Th17/Treg T cells in peripheral blood from patients with Graves' disease (GD; n = 29, mean age 15.4 ± 5.1 years), Hashimoto's thyroiditis (HT; n = 39, mean age 15.2 ± 4.1 years) and in healthy controls (n = 49, mean age 14.8 ± 3 years). Polychromatic flow cytometry and several fluorochrome-conjugated monoclonal antibodies were applied to delineate Th17 and Treg cells. The analysis of Th17/Treg T cell proportions in peripheral blood from patients with Graves' disease revealed significantly lower ratios of CD4 + IL17+/CD4 + CD25 + CD127 - (p < 0.0021) and CD4 + IL17+/CD4 + CD25 + CD127 - FoxP3 + (p < 0.0031) than in the control group. In addition, in the case of HT, we observed a significant decrease in the ratios of CD4 + IL17+/CD4 + CD25 + CD127 - (p < 0.0001) and CD4 + IL17+/CD4 + CD25 + CD127 - FoxP3 + (p < 0.0001) T cells in comparison to healthy children. In patients with untreated GD, a statistically significant positive correlation was found between the proportions of CD4 + IL17+/CD4 + CD25 + CD127-, CD4 + IL17+/CD4 + CD25 + CD127 - FoxP3+ T cells and the TRAbs (R = 0.71, p < 0.029; R = 0.72, p < 0.026, respectively) and a positive correlation was noted between the percentage of CD4 + CD - IL - 17 + T cells and the level of TSAbs (R = 0.66, p < 0.037). We conclude that the changes in the proportion of Th17/Treg T cells in peripheral blood and their significant relationship with the level of anti-thyroid antibodies indicate an involvement of these cells in the pathogenesis of AITD.
authors.affiliation: a Department of Pediatric Endocrinology , Diabetology with Cardiology Division, Medical University of Białystok , Białystok , Poland .
authors.forename: Artur
authors.initials: A
authors.lastname: Bossowski
authors.affiliation: b Department of Regenerative Medicine and Immune Regulation , Medical University of Bialystok , Białystok , Poland .
authors.forename: Marcin
authors.initials: M
authors.lastname: Moniuszko
authors.affiliation: a Department of Pediatric Endocrinology , Diabetology with Cardiology Division, Medical University of Białystok , Białystok , Poland .
authors.forename: Ewelina
authors.initials: E
authors.lastname: Idźkowska
authors.affiliation: b Department of Regenerative Medicine and Immune Regulation , Medical University of Bialystok , Białystok , Poland .
authors.forename: Kamil
authors.initials: K
authors.lastname: Grubczak
authors.affiliation: b Department of Regenerative Medicine and Immune Regulation , Medical University of Bialystok , Białystok , Poland .
authors.forename: Paulina
authors.initials: P
authors.lastname: Singh
authors.affiliation: c Division of Cardiology , Internal Affairs Ministry Hospital in Białystok , Białystok , Poland , and.
authors.forename: Anna
authors.initials: A
authors.lastname: Bossowska
authors.affiliation: d Molecular Thyroid Research Laboratory, Johannes Gutenberg University (JGU) Medical Center , Mainz , Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Diana
authors.affiliation: d Molecular Thyroid Research Laboratory, Johannes Gutenberg University (JGU) Medical Center , Mainz , Germany.
authors.forename: George J
authors.initials: GJ
authors.lastname: Kahaly
mesh_terms: D000293:Adolescent; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D015415:Biomarkers; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006111:Graves Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D050031:Hashimoto Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D018655:Lymphocyte Count; D008297:Male; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism; D013959:Thyroid Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D015415:Biomarkers
keywords: Graves’ disease; Hashimoto’s thyroiditis; Th17; Tregs; anti-thyroid antibodies