title: Transcriptional repression of p27 is essential for murine embryonic development.
abstract: The Nczf gene has been identified as one of Ncx target genes and encodes a novel KRAB zinc-finger protein, which functions as a sequence specific transcriptional repressor. In order to elucidate Nczf functions, we generated Nczf knockout (Nczf-/-) mice. Nczf-/- mice died around embryonic day 8.5 (E8.5) with small body size and impairment of axial rotation. Histopathological analysis revealed that the cell number decreased and pyknotic cells were occasionally observed. We examined the expression of cell cycle related genes in Nczf-/- mice. p27 expression was increased in E8.0 Nczf-/- mice compared to that of wild type mice. Nczf knockdown by siRNA resulted in increased expression of p27 in mouse embryonic fibroblasts (MEFs). Furthermore, p27 promoter luciferase reporter gene analysis confirmed the regulation of p27 mRNA expression by Nczf. Nczf-/-; p27-/- double knockout mice survived until E11.5 and the defect of axial rotation was restored. These data suggest that p27 repression by Nczf is essential in the developing embryo.
authors.affiliation: Department of Biomedical Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Teratake
authors.affiliation: Department of Biomedical Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Chisa
authors.initials: C
authors.lastname: Kuga
authors.affiliation: Department of Biomedical Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: Developmental Genetics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Developmental Genetics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kitahashi
authors.affiliation: Biomedical Research Center, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Lisa
authors.initials: L
authors.lastname: Fujimura
authors.affiliation: Biomedical Research Center, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Haruko
authors.initials: H
authors.lastname: Watanabe-Takano
authors.affiliation: Department of Biomedical Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.|Biomedical Research Center, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Sakamoto
authors.affiliation: Developmental Genetics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Arima
authors.affiliation: Developmental Genetics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tokuhisa
authors.affiliation: Department of Biomedical Science, Graduate School of Medicine, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.|Biomedical Research Center, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba city, Chiba, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hatano
mesh_terms: D000818:Animals; D002452:Cell Count; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000002:abnormalities; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011247:Pregnancy; D034622:RNA Interference; D014158:Transcription, Genetic
chemical_list: D050760:Cyclin-Dependent Kinase Inhibitor p27