title: [Molecular epidemiology of phlebovirus in four provinces in Morocco].
abstract: Sandflies are vectors of protozoa, viruses, and bacteria. To investigate the transmission of phleboviruses, a total of 8753 sandflies were collected in four foci of leishmania. A total of 16 distinct species were morphologically identified. Nested-PCR and cell culture screening for phleboviruses, using an assay targeting the polymerase gene, showed positive results for 19 pools of sandflies. Sequencing of the corresponding products confirmed the results and allowed identification of Toscana virus exclusively. Corresponding sandfly species originated from four different foci, and were different from those commonly reported in the literature. Sequence analysis shows that the Moroccan Toscana viruses belong to genotype B and appear close to the Toscana viruses isolated in France and Spain. This study reported the existence of the virus in the north, center and south of the country. The abundance and diversity of sandflies in Morocco, Mediterranean climate, would support the continuous circulation of Toscana virus in our country, posing a potential risk of emergence of these arboviruses.
authors.affiliation: Laboratoire de parasitologie et de maladies vectorielles, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360, Casablanca, Maroc. meryem.lemrani@pasteur.ma.
authors.forename: N
authors.initials: N
authors.lastname: Es-Sette
authors.affiliation: Laboratoire de parasitologie et de maladies vectorielles, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360, Casablanca, Maroc.
authors.forename: M
authors.initials: M
authors.lastname: Ajaoud
authors.affiliation: UMR EPV « Emergence des pathologies virales », Aix Marseille Université, IRD U190, INSERM U1207, IRBA, EFS, EHESP, Marseille, France.|France & Fondation Méditerranée Infection, APHM Assistance Publique - Hôpitaux de Marseille, Marseille, France.
authors.forename: R N
authors.initials: RN
authors.lastname: Charrel
authors.affiliation: Laboratoire de parasitologie et de maladies vectorielles, Institut Pasteur du Maroc, 1 Place Louis Pasteur, 20360, Casablanca, Maroc.
authors.forename: M
authors.initials: M
authors.lastname: Lemrani
mesh_terms: D000818:Animals; D002044:Bunyaviridae Infections / Q000453:epidemiology* / Q000821:virology*; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis; D004197:Disease Reservoirs / Q000706:statistics & numerical data / Q000821:virology; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000821:virology; D017720:Molecular Epidemiology; D009018:Morocco / Q000453:epidemiology; D010691:Phlebotomus / Q000821:virology*; D016856:Phlebovirus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D014709:Vero Cells
chemical_list: D004279:DNA, Viral
keywords: Agmeroul; Ait Makhlouf; Azilal; El Hanchane; Essaouira; Guimi; Louata; Maghreb; Meningitis; Morocco; Northern Africa; Ouarzazate; Phlebovirus; Sandflies; Sefrou; Toscana virus; Zaouit Sidi Othmane