title: A prospective randomized study comparing two commercially available types of human embryo culture media: G1-PLUS™/G2-PLUS™ sequential medium (Vitrolife) and the GL BLAST™ sole medium (Ingamed).
abstract: OBJECTIVE
  To check the efficacy of two types of commercially available embryo culture medium: G1-PLUS™/G2-PLUS™ sequential (Vitrolife, Gothenburg, Sweden) and GV BLAST™ sole (Ingamed, Maringá, Brazil) with regards to fertilization, cleavage, blastocyst and pregnancy rates.
  
  
  METHODS
  Prospective and randomized study conducted from March to July 2015, using the medical records of 60 patients submitted to Intracytoplasmic Sperm Injection techniques (ICSI). Data regarding the age of patients, together with fertilization, cleavage, blastocyst and pregnancy rates, were collected and compared in relation to the: G1-PLUS™/G2-PLUS™ sequential and GV BLAST™ sole mediums. The data were tabulated and compared using the Pearson's Chi-Square test (95% CI).
  
  
  RESULTS
  There was no significant difference when comparing patients divided into higher and lower fertility age. No significant statistical difference was noted between the fertilization rates (P=0.59), cleavage (P=0.91), evolution to blastocyst (P=0.33) and total pregnancy (P=0.83) when comparing the embryos cultured in the different media analysed.
  
  
  CONCLUSIONS
  We conclude that the G1-PLUS™/G2-PLUS™ sequential and GV BLAST™ sole mediums are equally effective with regards to fertilization, cleavage, blastocyst development and total pregnancy rates.
authors.affiliation: Universidade Positivo, Setor de Ciências Biológicas e da Saúde, Curitiba, Paraná, Brazil.
authors.forename: Ianae I
authors.initials: II
authors.lastname: Ceschin
authors.affiliation: Universidade Positivo, Setor de Ciências Biológicas e da Saúde, Curitiba, Paraná, Brazil.
authors.forename: Mariana H
authors.initials: MH
authors.lastname: Ribas
authors.affiliation: Feliccitá Instituto de Fertilidade, Curitiba, Paraná, Brazil.|Universidade de Brasília, Departamento de Genética e Morfologia, Brasília, Federal District, Brazil.
authors.forename: Alvaro P
authors.initials: AP
authors.lastname: Ceschin
authors.affiliation: Feliccitá Instituto de Fertilidade, Curitiba, Paraná, Brazil.
authors.forename: Lucileine
authors.initials: L
authors.lastname: Nishikawa
authors.affiliation: Feliccitá Instituto de Fertilidade, Curitiba, Paraná, Brazil.
authors.forename: Claudia C
authors.initials: CC
authors.lastname: Rocha
authors.affiliation: Universidade de Brasília, Departamento de Genética e Morfologia, Brasília, Federal District, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Pic-Taylor
authors.affiliation: Universidade Positivo, Setor de Ciências Biológicas e da Saúde, Curitiba, Paraná, Brazil.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Baroneza
mesh_terms: D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000187:drug effects* / Q000706:statistics & numerical data*
chemical_list: D003470:Culture Media
keywords: Embryonic culture medium; ICSI; In vitro fertilization; Pregnancy rate