title: Genetic variants in ATP6 and ND3 mitochondrial genes are not associated with aggressive prostate cancer in Mexican-Mestizo men with overweight or obesity.
abstract: Mitochondrial defects have been related to obesity and prostate cancer. We investigated if Mexican-Mestizo men presenting this type of cancer, exhibited somatic mutations of ATP6 and/or ND3.Body mass index (BMI) was determined; the degree of prostate cancer aggressiveness was demarcated by the Gleason score. DNA from tumor tissue and from blood leukocytes was amplified by the polymerase chain reaction and ATP6 and ND3 were sequenced. We included 77 men: 20 had normal BMI, 38 were overweight and 19 had obesity; ages ranged from 52 to 83. After sequencing ATP6 and ND3, from DNA obtained from leukocytes and tumor tissue, we did not find any somatic mutations. All changes observed, in both genes, were polymorphisms. In ATP6 we identified, in six patients, two non-synonymous nucleotide changes and in ND3 we observed that twelve patients presented non-synonymous polymorphisms. To our knowledge, this constitutes the first report where the complete sequences of the ATP6 and ND3 have been analyzed in Mexican-Mestizo men with prostate cancer and diverse BMI. Our results differ with those reported in Caucasian populations, possibly due to ethnic differences.
authors.affiliation: a Facultad de Medicina , Unidad de Investigación en Obesidad, Universidad Nacional Autónoma de México , México, D.F , México.|b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: Patricia
authors.initials: P
authors.lastname: Canto
authors.affiliation: a Facultad de Medicina , Unidad de Investigación en Obesidad, Universidad Nacional Autónoma de México , México, D.F , México.|b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: Jesús
authors.initials: J
authors.lastname: Benítez Granados
authors.affiliation: a Facultad de Medicina , Unidad de Investigación en Obesidad, Universidad Nacional Autónoma de México , México, D.F , México.|b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: Mónica Adriana
authors.initials: MA
authors.lastname: Martínez Ramírez
authors.affiliation: c Departamento de Patología , Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Reyes
authors.affiliation: d Departamento de Urología , Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México , and.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Feria-Bernal
authors.affiliation: b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-García
authors.affiliation: a Facultad de Medicina , Unidad de Investigación en Obesidad, Universidad Nacional Autónoma de México , México, D.F , México.|b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Tejeda
authors.affiliation: a Facultad de Medicina , Unidad de Investigación en Obesidad, Universidad Nacional Autónoma de México , México, D.F , México.|b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Zavala
authors.affiliation: a Facultad de Medicina , Unidad de Investigación en Obesidad, Universidad Nacional Autónoma de México , México, D.F , México.|b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: André
authors.initials: A
authors.lastname: Tapia
authors.affiliation: e Unidad de Medicina Física y Rehabilitación Centro, UMAE, Hospital de Traumatología y Ortopedia "Lomas Verdes", Instituto Mexicano del Seguro Social , México, D.F , México.
authors.forename: David
authors.initials: D
authors.lastname: Rojano-Mejía
authors.affiliation: a Facultad de Medicina , Unidad de Investigación en Obesidad, Universidad Nacional Autónoma de México , México, D.F , México.|b Clínica de Obesidad, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán" , México, D.F , México.
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Méndez
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D042967:Electron Transport Complex I / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics / Q000502:physiology*; D009362:Neoplasm Metastasis / Q000235:genetics; D009765:Obesity / Q000150:complications / Q000235:genetics*; D050177:Overweight / Q000150:complications / Q000235:genetics*; D011110:Polymorphism, Genetic; D011471:Prostatic Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology
chemical_list: C501005:MT-ATP6 protein, human; D025261:Mitochondrial Proton-Translocating ATPases; D042967:Electron Transport Complex I; C114961:MT-ND3 protein, human
keywords: ATP6; Aggressive prostate cancer; Mexican–Mestizo men; ND3; genetic variants