title: Programming Surface Chemistry with Engineered Cells.
abstract: We have developed synthetic gene networks that enable engineered cells to selectively program surface chemistry. E. coli were engineered to upregulate biotin synthase, and therefore biotin synthesis, upon biochemical induction. Additionally, two different functionalized surfaces were developed that utilized binding between biotin and streptavidin to regulate enzyme assembly on programmable surfaces. When combined, the interactions between engineered cells and surfaces demonstrated that synthetic biology can be used to engineer cells that selectively control and modify molecular assembly by exploiting surface chemistry. Our system is highly modular and has the potential to influence fields ranging from tissue engineering to drug development and delivery.
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University , Blacksburg, Virginia 24061, United States.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University , Blacksburg, Virginia 24061, United States.
authors.forename: Keith C
authors.initials: KC
authors.lastname: Heyde
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University , Blacksburg, Virginia 24061, United States.
authors.forename: Felicia Y
authors.initials: FY
authors.lastname: Scott
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University , Blacksburg, Virginia 24061, United States.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Paek
authors.affiliation: Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University , Blacksburg, Virginia 24061, United States.
authors.forename: Warren C
authors.initials: WC
authors.lastname: Ruder
mesh_terms: D001710:Biotin / Q000378:metabolism; D060846:Cell Engineering / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D053263:Gene Regulatory Networks / Q000235:genetics; D019809:Streptavidin / Q000378:metabolism; D013466:Sulfurtransferases / Q000235:genetics; D058615:Synthetic Biology / Q000379:methods; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001710:Biotin; D019809:Streptavidin; D013466:Sulfurtransferases; C057985:biotin synthetase