title: Roe Protein Hydrolysates of Giant Grouper (Epinephelus lanceolatus) Inhibit Cell Proliferation of Oral Cancer Cells Involving Apoptosis and Oxidative Stress.
abstract: Roe protein hydrolysates were reported to have antioxidant property but the anticancer effects were less addressed, especially for oral cancer. In this study, we firstly used the ultrafiltrated roe hydrolysates (URH) derived from giant grouper (Epinephelus lanceolatus) to evaluate the impact of URH on proliferation against oral cancer cells. We found that URH dose-responsively reduced cell viability of two oral cancer cells (Ca9-22 and CAL 27) in terms of ATP assay. Using flow cytometry, URH-induced apoptosis of Ca9-22 cells was validated by morphological features of apoptosis, sub-G1 accumulation, and annexin V staining in dose-responsive manners. URH also induced oxidative stress in Ca9-22 cells in terms of reactive oxygen species (ROS)/superoxide generations and mitochondrial depolarization. Taken together, these data suggest that URH is a potential natural product for antioral cancer therapy.
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 81157, Taiwan.
authors.forename: Jing-Iong
authors.initials: JI
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan; Department of Radiation Oncology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 80708, Taiwan.
authors.forename: Jen-Yang
authors.initials: JY
authors.lastname: Tang
authors.affiliation: Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 81157, Taiwan.
authors.forename: Ya-Sin
authors.initials: YS
authors.lastname: Liu
authors.affiliation: Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors.forename: Hui-Ru
authors.initials: HR
authors.lastname: Wang
authors.affiliation: School of Dentistry, Taipei Medical University, Taipei 11031, Taiwan; Division of Orthodontics, Wan-Fang Medical Center, Taipei Medical University, Taipei 11648, Taiwan.
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Dentistry, Taipei Medical University, Taipei 11031, Taiwan; Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Tainan 71004, Taiwan.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Yen
authors.affiliation: Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Center for Research Resources and Development of Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors.forename: Hsueh-Wei
authors.identifier: 0000-0003-0068-2366
authors.initials: HW
authors.lastname: Chang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D053078:Membrane Potential, Mitochondrial; D009062:Mouth Neoplasms / Q000473:pathology; D010063:Ovum / Q000737:chemistry*; D018384:Oxidative Stress; D010473:Perciformes; D011492:Protein Hydrolysates / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014462:Ultrafiltration
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D013481:Superoxides