title: Identification of human RNA editing sites: A historical perspective.
abstract: A-to-I RNA editing is an essential gene regulatory mechanism. Once thought to be a rare phenomenon only occurring in a few transcripts, the emergence of high-throughput RNA sequencing has facilitated the identification of over 2 million RNA editing sites in the human transcriptome. In this review, we survey the current RNA-seq based methods as well as historical methods used to identify RNA editing sites.
authors.affiliation: Department of Genetics, Stanford University, Stanford, CA 94305, USA; Program in Neurogenetics, Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: GRamaswami@mednet.ucla.edu.
authors.forename: Gokul
authors.initials: G
authors.lastname: Ramaswami
authors.affiliation: Department of Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address: jin.billy.li@stanford.edu.
authors.forename: Jin Billy
authors.initials: JB
authors.lastname: Li
mesh_terms: D015894:Genome, Human / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D017393:RNA Editing / Q000235:genetics*; D017423:Sequence Analysis, RNA; D059467:Transcriptome / Q000235:genetics*
keywords: Post-transcriptional gene regulation; RNA editing; RNA sequencing