title: Identification of proliferative progenitors associated with prominent postnatal growth of the pons.
abstract: The pons controls crucial sensorimotor and autonomic functions. In humans, it grows sixfold postnatally and is a site of paediatric gliomas; however, the mechanisms of pontine growth remain poorly understood. We show that the murine pons quadruples in volume postnatally; growth is fastest during postnatal days 0-4 (P0-P4), preceding most myelination. We identify three postnatal proliferative compartments: ventricular, midline and parenchymal. We find no evidence of postnatal neurogenesis in the pons, but each progenitor compartment produces new astroglia and oligodendroglia; the latter expand 10- to 18-fold postnatally, and are derived mostly from the parenchyma. Nearly all parenchymal progenitors at P4 are Sox2(+)Olig2(+), but by P8 a Sox2(-) subpopulation emerges, suggesting a lineage progression from Sox2(+) 'early' to Sox2(-) 'late' oligodendrocyte progenitor. Fate mapping reveals that >90% of adult oligodendrocytes derive from P2-P3 Sox2(+) progenitors. These results demonstrate the importance of postnatal Sox2(+)Olig2(+) progenitors in pontine growth and oligodendrogenesis.
authors.affiliation: Eli &Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California-San Francisco, San Francisco, California 94143, USA.|Medical Scientist Training Program, University of California-San Francisco, San Francisco, California 94143, USA.|Neuroscience Graduate Program, University of California-San Francisco, San Francisco, California 94143, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Lindquist
authors.affiliation: Eli &Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California-San Francisco, San Francisco, California 94143, USA.|Department of Neurological Surgery, University of California-San Francisco, San Francisco, California 94143, USA.
authors.forename: Cristina D
authors.initials: CD
authors.lastname: Guinto
authors.affiliation: Eli &Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California-San Francisco, San Francisco, California 94143, USA.|Department of Neurological Surgery, University of California-San Francisco, San Francisco, California 94143, USA.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Rodas-Rodriguez
authors.affiliation: Eli &Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California-San Francisco, San Francisco, California 94143, USA.|Department of Neurological Surgery, University of California-San Francisco, San Francisco, California 94143, USA.
authors.forename: Luis C
authors.initials: LC
authors.lastname: Fuentealba
authors.affiliation: Eli &Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California-San Francisco, San Francisco, California 94143, USA.|Department of Neurological Surgery, University of California-San Francisco, San Francisco, California 94143, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Tate
authors.affiliation: Eli &Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California-San Francisco, San Francisco, California 94143, USA.|Department of Pediatrics, University of California-San Francisco, San Francisco, California 94143, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Rowitch
authors.affiliation: Eli &Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California-San Francisco, San Francisco, California 94143, USA.|Department of Neurological Surgery, University of California-San Francisco, San Francisco, California 94143, USA.
authors.forename: Arturo
authors.initials: A
authors.lastname: Alvarez-Buylla
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D049109:Cell Proliferation; D020546:Fourth Ventricle / Q000166:cytology; D051379:Mice; D055495:Neurogenesis; D000073637:Oligodendrocyte Precursor Cells / Q000502:physiology*; D000076104:Oligodendrocyte Transcription Factor 2 / Q000378:metabolism; D009836:Oligodendroglia / Q000502:physiology; D011149:Pons / Q000166:cytology / Q000254:growth & development*; D055748:SOXB1 Transcription Factors / Q000378:metabolism
chemical_list: C512773:Olig2 protein, mouse; D000076104:Oligodendrocyte Transcription Factor 2; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse