title: Human aqueous humor proteome in cataract, glaucoma, and pseudoexfoliation syndrome.
abstract: Twenty-nine human aqueous humor samples from patients with eye diseases such as cataract and glaucoma with and without pseudoexfoliation syndrome were characterized by LC-high resolution MS analysis. In total, 269 protein groups were identified with 1% false discovery rate including 32 groups that were not reported previously for this biological fluid. Since the samples were analyzed individually, but not pooled, 36 proteins were identified in all samples, comprising the constitutive proteome of the fluid. The most dominant molecular function of aqueous humor proteins as determined by GO analysis is endopeptidase inhibitor activity. Label-free protein quantification showed no significant difference between glaucoma and cataract aqueous humor proteomes. At the same time, we found decrease in the level of apolipoprotein D as a marker of the pseudoexfoliation syndrome. The data are available from ProteomeXchange repository (PXD002623).
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Kliuchnikova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.|Russian Medical Academy of Postgraduate Education, Moscow, Russia.
authors.forename: Nadezhda I
authors.initials: NI
authors.lastname: Samokhina
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Irina Y
authors.initials: IY
authors.lastname: Ilina
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.|Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Dmitry S
authors.initials: DS
authors.lastname: Karpov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.|Pirogov Russian National Research Medical University (RNRMU), Moscow, Russia.
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Pyatnitskiy
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Ksenia G
authors.initials: KG
authors.lastname: Kuznetsova
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Ilya Y
authors.initials: IY
authors.lastname: Toropygin
authors.affiliation: Russian Medical Academy of Postgraduate Education, Moscow, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Kochergin
authors.affiliation: Russian Medical Academy of Postgraduate Education, Moscow, Russia.
authors.forename: Igor B
authors.initials: IB
authors.lastname: Alekseev
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Victor G
authors.initials: VG
authors.lastname: Zgoda
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia.|Pirogov Russian National Research Medical University (RNRMU), Moscow, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Moshkovskii
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D053399:Apolipoproteins D / Q000032:analysis; D001082:Aqueous Humor / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D002386:Cataract / Q000175:diagnosis*; D002853:Chromatography, Liquid; D017889:Exfoliation Syndrome / Q000175:diagnosis*; D005901:Glaucoma / Q000175:diagnosis*; D006801:Humans; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D053399:Apolipoproteins D; D015415:Biomarkers; D020543:Proteome
keywords: Aqueous humor; Biomedicine; Cataract; Glaucoma; Pseudoexfoliation syndrome; Shotgun proteomics