title: A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer.
abstract: To enhance the diagnosis of non-small cell lung cancer (NSCLC), we prepared a dual-modal probe Cy5.5-Tf-Gd-DTPA. Gd-DTPA and near-infrared (NIR) dyes were conjugated to holo-Transferrin (Tf) sequentially, the result of ICP-AES and UV showed 25 Gd ions and 1 Cy5.5 could be loaded per protein, respectively. The calculated longitudinal relaxivity R1 of Cy5.5-Tf-DTPA-Gd was 4.21 mM-1S-1 per Gd while that of Magnevist (Gd-DTPA) was only 4.02 mM-1S-1. Confocal laser scanning microscopy and immunohistochemical analyses revealed that the Cy5.5-Tf-DTPA-Gd was localized and accumulated in cytoplasmic vesicles; the cell toxicity assay showed no apparent toxicity. MR and NIR imaging of mice with subcutaneous H1299 xenografte tumors following intravenous injection of Cy5.5-Tf-DTPA-Gd revealed a strong positive contrast of the tumors, which caused a longer lasting enhancement of the MRI signal and fluorescence signal. Taken together, these studies indicate that Cy5.5-Tf-DTPA-Gd could be a good agent for MR/NIRF dual mode applications to detect both tumor in situ and its metastasis.
authors.affiliation: Department of Radiology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai, China.|Center for Biomedical Imaging, Fudan University, Shanghai, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.|Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai, China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Gu
authors.affiliation: School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Fengwen
authors.initials: F
authors.lastname: Cao
authors.affiliation: Department of Radiology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007477:Ions; D008175:Lung Neoplasms / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D019265:Spectroscopy, Near-Infrared; D014168:Transferrin / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007477:Ions; D014168:Transferrin; D005682:Gadolinium
keywords: MRI/NIRF; liver metastasis; molecular imaging; non-small cell lung cancer; transferrin