title: Marinirhabdus gelatinilytica gen. nov., sp. nov., isolated from seawater.
abstract: A Gram-stain-negative, aerobic and slightly halophilic bacterium was isolated from the South China Sea, and was subjected to characterization using a polyphasic taxonomic approach. Cells of the isolate, designated NH83T, were non-motile and rod-shaped. On the basis of 16S rRNA gene sequence analysis, strain NH83Twas closely related to members of the genera Aureisphaera (with sequence similarity of 92.9 %), Jejudonia (92.8 %), Marixanthomonas (92.6 %), Altuibacter (92.6 %), Ulvibacter (91.5-91.9 %), Gilvibacter (91.8 %) and Aequorivita (89.6-91.2 %), all of which belong to the family Flavobacteriaceae. Phylogenetic analysis indicated that it represented an independent lineage and its closest relatives belonged to the genus Marixanthomonas. The sole respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, two aminolipids, one aminophospholipid and one unidentified lipid. The principal fatty acids were branched fatty acids, including iso-C15 : 0, iso-C17 : 0 3-OH, iso-C16 : 0, iso-C15 : 1 G and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). The genomic DNA G+C content was 41.0 mol%. Strain NH83T was positive for hydrolysis of aesculin, gelatin and Tween 60. Phylogenetic distinctiveness and chemotaxonomic differences, together with differential phenotypic properties, revealed that strain NH83T could be differentiated from closely related genera. Therefore, it is proposed that strain NH83T represents a novel species in a new genus, for which the name Marinirhabdus gelatinilytica gen. nov., sp. nov. (type strain NH83T=CGMCC 1.15462T=DSM 101478T) is proposed.
authors.affiliation: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Wu
authors.affiliation: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.|College of Life Science, Xinjiang Normal University, Urumqi 830054, PR China.
authors.forename: Maripat
authors.initials: M
authors.lastname: Xamxidin
authors.affiliation: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Fan-Xu
authors.initials: FX
authors.lastname: Meng
authors.affiliation: College of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, Lin'an 311300, PR China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Wang
authors.affiliation: College of Life Science, Xinjiang Normal University, Urumqi 830054, PR China.
authors.forename: Dilbar
authors.initials: D
authors.lastname: Tohty
authors.affiliation: Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6