title: Complement components of nerve regeneration conditioned fluid influence the microenvironment of nerve regeneration.
abstract: Nerve regeneration conditioned fluid is secreted by nerve stumps inside a nerve regeneration chamber. A better understanding of the proteinogram of nerve regeneration conditioned fluid can provide evidence for studying the role of the microenvironment in peripheral nerve regeneration. In this study, we used cylindrical silicone tubes as the nerve regeneration chamber model for the repair of injured rat sciatic nerve. Isobaric tags for relative and absolute quantitation proteomics technology and western blot analysis confirmed that there were more than 10 complement components (complement factor I, C1q-A, C1q-B, C2, C3, C4, C5, C7, C8β and complement factor D) in the nerve regeneration conditioned fluid and each varied at different time points. These findings suggest that all these complement components have a functional role in nerve regeneration.
authors.affiliation: Plastic & Reconstructive Surgery of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Guang-Shuai
authors.initials: GS
authors.lastname: Li
authors.affiliation: Plastic & Reconstructive Surgery of the Ninth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Li
authors.affiliation: Otolaryngology-Head & Neck Surgery of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Ming-Min
authors.identifier: 0000-0001-5329-4709
authors.initials: MM
authors.lastname: Dong
authors.affiliation: Plastic & Reconstructive Surgery of the Ninth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zan
authors.affiliation: Plastic & Reconstructive Surgery of the Ninth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Ding
authors.affiliation: Plastic & Reconstructive Surgery of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Lin-Bo
authors.initials: LB
authors.lastname: Liu
keywords: complement; iTRAQ proteomics technology; nerve regeneration; nerve regeneration chamber model; nerve regeneration conditioned fluid; neural regeneration; peripheral nerve injury; sciatic nerve