title: Phytochemical Composition and Antibacterial Activity of Hydroalcoholic Extracts of Pterospartum tridentatum and Mentha pulegium against Staphylococcus aureus Isolates.
abstract: Pterospartum tridentatum and Mentha pulegium are largely used in Portuguese folk medicine to treat several human disorders and inflammatory processes but without any consistent evidence for those beneficial pointed properties. Thus, the aim of the current work is to evaluate its benefits and phytochemicals related to those beneficial properties. A distinct polyphenol profile between P. tridentatum and M. pulegium was found. Taxifolin, myricetin, ginestin, ginestein, and ginestein derivatives, biochanin A-glucoside, and biochanin A were identified in P. tridentatum, whilst in M. pulegium the luteolin-7-rutinoside, diosmin, and apigenin and respective derivatives were most representative polyphenols. These variations had implications in the antiradical and antibacterial activity and the P. tridentatum exhibited the highest antibacterial activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus MSSA, which was mainly dose-dependent. This antibacterial activity seems to be related to high content of flavonols, flavones, and isoflavones, which can act synergistically with each other against this type of bacteria. Our results showed consistent evidence that Pterospartum tridentatum and Mentha pulegium are an important reservoir of phytochemicals with antiradical activity and antibacterial capacity and thus they might be used in a preventive way or in a combined pharmaceutical and antibiotic therapy against pathogenic bacteria.
authors.affiliation: Centre for the Research and Technology for Agro-Environment and Biological Sciences, CITAB, University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5001-801 Vila Real, Portugal.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Aires
authors.affiliation: University of Trás-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Marrinhas
authors.affiliation: University of Trás-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal.
authors.forename: Rosa
authors.initials: R
authors.lastname: Carvalho
authors.affiliation: Animal and Veterinary Research Centre, CECAV, University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5001-801 Vila Real, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Dias
authors.affiliation: Animal and Veterinary Research Centre, CECAV, University of Trás-os-Montes e Alto Douro, Quinta de Prados, 5001-801 Vila Real, Portugal.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Saavedra
mesh_terms: D047309:Flavones / Q000737:chemistry / Q000494:pharmacology; D044948:Flavonols / Q000737:chemistry / Q000494:pharmacology; D039761:Genista / Q000737:chemistry; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology; D031335:Mentha pulegium / Q000737:chemistry; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000472:pathogenicity; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D047309:Flavones; D044948:Flavonols; D007529:Isoflavones; D010936:Plant Extracts; D059808:Polyphenols; C065753:isoflavanone