title: Gentamicin Induced Nephrotoxicity: The Role of Sex Hormones in Gonadectomized Male and Female Rats.
abstract: Background. Gentamicin (GM) induced nephrotoxicity may be sex hormones related. The effects of sex hormones on GM induced nephrotoxicity in gonadectomized rats were investigated. Methods. Ovariectomized rats received 0.25, 0.5, or 1 mg/kg/week of estradiol (ES) alone or accompanied with 10 mg/kg/week of progesterone (Pro) for two weeks followed by GM (100 mg/kg/day) for 9 days. Castrated rats were also treated with 10, 50, or 100 mg/kg/week of testosterone (TS) for two weeks and then received GM. In addition, a single castrated group received 0.25 mg/kg/week of ES plus GM. Results. GM increased the serum levels of blood urea nitrogen (BUN) and creatinine (Cr) and kidney tissue damage score (KTDS) (P < 0.05). TS had no effect on the serum levels of BUN and Cr and KTDS, while low dose of ES intensified these parameters in male (P < 0.05). ES (0.5 mg/kg) without Pro ameliorated KTDS in female (P < 0.05) while ES (1 mg/kg) with or without Pro exacerbated the BUN values and Cr values, KTDS, and body weight loss (P < 0.05). Conclusion. ES (0.5 mg/kg) without Pro ameliorated kidney damage induced by GM in female while neither TS nor ES had beneficial effect on nephrotoxicity induced by GM in male, although ES aggravated it.
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Eshraghi-Jazi
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran; Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan 81745, Iran.
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Talebi
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran.
authors.forename: Fatemeh Sadat
authors.identifier: 0000-0002-3865-8348
authors.initials: FS
authors.lastname: Mirsaeedi
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran.
authors.forename: Sarina
authors.identifier: 0000-0002-8711-1453
authors.initials: S
authors.lastname: Ahmadian
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Moslemi
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran; Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745, Iran; Isfahan-MN Institute of Basic and Applied Sciences Research, Isfahan 81546, Iran.
authors.forename: Mehdi
authors.identifier: 0000-0002-9565-9187
authors.initials: M
authors.lastname: Nematbakhsh