title: Reconstructing the Phylogeny of Capsosiphon fulvescens (Ulotrichales, Chlorophyta) from Korea Based on rbcL and 18S rDNA Sequences.
abstract: Capsosiphon fulvescens is a filamentous green algae in the class Ulvophyceae. It has been consumed as food with unique flavor and soft texture to treat stomach disorders and hangovers, and its economic value justifies studying its nutritional and potential therapeutic effects. In contrast to these applications, only a few taxonomic studies have been conducted on C. fulvescens. In particular, classification and phylogenetic relationships of the C. fulvescens below the order level are controversial. To determine its phylogenetic position in the class, we used rbcL and 18S rDNA sequences as molecular markers to construct phylogenetic trees. The amplified rbcL and 18S rDNA sequences from 4 C. fulvescens isolates (Jindo, Jangheung, Wando, and Koheung, Korea) were used for phylogenetic analysis by employing three different phylogenetic methods: neighbor joining (NJ), maximum parsimony (MP), and maximum likelihood (ML). The rbcL phylogenetic tree showed that all taxa in the order Ulvales were clustered as a monophyletic group and resolved the phylogenetic position of C. fulvescens in the order Ulotrichales. The significance of our study is that the 18S rDNA phylogenetic tree shows the detailed taxonomic position of C. fulvescens. In our result, C. fulvescens is inferred as a member of Ulotrichaceae, along with Urospora and Acrosiphonia.
authors.affiliation: Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626, Republic of Korea.
authors.forename: Sang-Mi
authors.initials: SM
authors.lastname: Sun
authors.affiliation: Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Gyeonggi 17058, Republic of Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Educational Scientific Center of Nanotechnology, Far Eastern Federal University, Vladivostok 690-950, Russia.
authors.forename: Kirill S
authors.identifier: 0000-0002-4873-2281
authors.initials: KS
authors.lastname: Golokhvast
authors.affiliation: Department of Biomedical and Electronic Engineering, Chonnam National University, Yeosu, Chonnam 59626, Republic of Korea.
authors.forename: Bao
authors.identifier: 0000-0001-6037-0546
authors.initials: B
authors.lastname: Le
authors.affiliation: Department of Biotechnology, Chonnam National University, Yeosu, Chonnam 59626, Republic of Korea.
authors.forename: Gyuhwa
authors.identifier: 0000-0001-8053-689X
authors.initials: G
authors.lastname: Chung
mesh_terms: D001483:Base Sequence; D000460:Chlorophyta / Q000235:genetics*; D048028:DNA, Algal / Q000235:genetics*; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D048028:DNA, Algal; D012337:RNA, Ribosomal, 18S; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase