title: Characterization of LysPBC4, a novel Bacillus cereus-specific endolysin of bacteriophage PBC4.
abstract: Bacillus cereus is a spore-forming, Gram-positive bacterium and is a major food-borne pathogen. A B. cereus-specific bacteriophage PBC4 was isolated from the soil of a stock farm, and its genome was analyzed. PBC4 belongs to the Siphoviridae family and has a genome consisting of 80 647-bp-long double-stranded DNA, including 123 genes and two tRNAs. LysPBC4, the endolysin of PBC4, has an enzymatically active domain (EAD) on its N-terminal region and a putative cell wall-binding domain (CBD) on its C-terminal region, respectively. Although the phage PBC4 showed a very limited host range, LysPBC4 could lyse all of the B. cereus strains tested. However, LysPBC4 did not kill other bacteria such as B. subtilis or Listeria, indicating that the endolysin has specific lytic activity against the B. cereus group species. Furthermore, LysPBC4_CBD fused with enhanced green fluorescent protein (EGFP) could decorate limited strains of B. cereus group, suggesting that the LysPBC4_CBD may be a promising material for specific detection of B. cereus.
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Na
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Minsuk
authors.initials: M
authors.lastname: Kong
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea sangryu@snu.ac.kr.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
mesh_terms: D017103:Bacillus Phages / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001409:Bacillus cereus / Q000302:isolation & purification / Q000502:physiology / Q000821:virology*; D001412:Bacillus subtilis / Q000502:physiology; D001433:Bacteriolysis; D010450:Endopeptidases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D005516:Food Microbiology; D016679:Genome, Viral; D049452:Green Fluorescent Proteins; D058507:Host Specificity; D008087:Listeria / Q000502:physiology; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000201:enzymology* / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D010450:Endopeptidases; C001143:endolysin
keywords: Bacillus cereus; bacteriophage; endolysin; foodborne disease