title: Cell-Cell Communication Between Fibroblast and 3T3-L1 Cells Under Co-culturing in Oxidative Stress Condition Induced by H2O2.
abstract: The present study was carried out to understand the interaction between fibroblast and 3T3-L1 preadipocyte cells under H2O2-induced oxidative stress condition. H2O2(40 μM) was added in co-culture and monoculture of fibroblast and 3T3-L1 cell. The cells in the lower well were harvested for analysis and the process was carried out for both cells. The cell growth, oxidative stress markers, and antioxidant enzymes were analyzed. Additionally, the mRNA expressions of caspase-3 and caspase-7 were selected for analysis of apoptotic pathways and TNF-α and NF-κB were analyzed for inflammatory pathways. The adipogenic marker such as adiponectin and PPAR-γ and collagen synthesis markers such as LOX and BMP-1 were analyzed in the co-culture of fibroblast and 3T3-L1 cells. Cell viability and antioxidant enzymes were significantly increased in the co-culture compared to the monoculture under stress condition. The apoptotic, inflammatory, adipogenic, and collagen-synthesized markers were significantly altered in H2O2-induced co-culture of fibroblast and 3T3-L1 cells when compared with the monoculture of H2O2-induced fibroblast and 3T3-L1 cells. In addition, the confocal microscopical investigation indicated that the co-culture of H2O2-induced 3T3-L1 and fibroblast cells increases collagen type I and type III expression. From our results, we suggested that co-culture of fat cell (3T3-L1) and fibroblast cells may influence/regulate each other and made the cells able to withstand against oxidative stress and aging. It is conceivable that the same mechanism might have been occurring from cell to cell while animals are stressed by various environmental conditions.
authors.affiliation: Department of Animal Science and BK21 PLUS Program, Chonbuk National University, Jeonju, 561-756, South Korea.
authors.forename: Sivakumar Allur
authors.initials: SA
authors.lastname: Subramaniyan
authors.affiliation: Animal Research Institute, National Institute of Animal Science, Daegangrungmyun Pyuygchanggun, Kwangwondo, Seoul, 25340, South Korea.
authors.forename: Sidong
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Animal Science and BK21 PLUS Program, Chonbuk National University, Jeonju, 561-756, South Korea. inho.hwang@jbnu.ac.kr.
authors.forename: Inho
authors.initials: I
authors.lastname: Hwang
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D002417:Cattle; D002450:Cell Communication / Q000187:drug effects*; D018920:Coculture Techniques / Q000379:methods*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007150:Immunohistochemistry; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D047495:PPAR gamma / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D024042:Collagen Type I; D024061:Collagen Type III; D016328:NF-kappa B; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha; D006861:Hydrogen Peroxide; D053148:Caspase 3; D053179:Caspase 7
keywords: Adipocytes; Apoptosis and inflammation; Co-culture; Fibroblast