title: Pro-inflammatory Cytokines Are Involved in Fluoride-Induced Cytotoxic Potential in HeLa Cells.
abstract: This study was designed to investigate the pro-inflammatory cytokines and their involvement in the cytotoxic potential of fluoride (F) in HeLa cells. HeLa cells were cultured with varying F concentrations (1-50 mg/L) for 48 h, and treatment effects were analyzed. The viability of HeLa cells was determined with a colorimetric method. The concentrations of IL-1β, IL-2, IL-6, and TNF-a in culture supernatant were measured through enzyme linked immunosorbent assay (ELISA). The mRNA expression levels of IL-1β, IL-2, IL-6 and TNF-a were subjected to transcript analysis and quantified through reverse transcription real-time PCR. Results showed that 10, 20 and 50 mg/L F significantly decreased the viability of HeLa cells incubated for 24 and 48 h. With their cytotoxic effect, the concentrations of IL-1β, IL-2, IL-6, and TNF-a decreased significantly in response to F, especially at 20 and 50 mg/L for 48 h. The mRNA expression levels of IL-1β, IL-2, IL-6, and TNF-a were downregulated at 50 mg/L F for 48 h. Therefore, F inhibited HeLa cell growth; as such, F could be used to alleviate the inhibition of pro-inflammatory cytokine expression.
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang, 471003, People's Republic of China. sxwhw@126.com.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang, 471003, People's Republic of China.
authors.forename: Bian-Hua
authors.initials: BH
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang, 471003, People's Republic of China.
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Cao
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang, 471003, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang, 471003, People's Republic of China.
authors.forename: Wen-Peng
authors.initials: WP
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Henan, Luoyang, 471003, People's Republic of China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Tan
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D016207:Cytokines / Q000096:biosynthesis*; D003603:Cytotoxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans
chemical_list: D016207:Cytokines; D003603:Cytotoxins; D005459:Fluorides
keywords: Cytokines; Fluoride; HeLa cells; TNF-a