title: Coassembly of Tobacco Mosaic Virus Coat Proteins into Nanotubes with Uniform Length and Improved Physical Stability.
abstract: Using tobacco mosaic virus coat proteins (TMVcp) from both sources of the plant and bacterial expression systems as building blocks, we demonstrate here a coassembly strategy of TMV nanotubes in the presence of RNA. Specifically, plant-expressed cp (cpp) efficiently dominates the genomic RNA encapsidation to determine the length of assembled TMV nanotubes, whereas the incorporated Escherichia coli-expressed cp (cpec) improves the physical stability of TMV nanotubes by introducing disulfide bonds between the interfaces of subunits. We expect this coassembly strategy can be expanded to other virus nanomaterials to obtain desired properties based on rationally designed protein-RNA and protein-protein interfacial interactions.
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Molecular Biology and Plant Virology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart , Pfaffenwaldring 57, D-70569 Stuttgart, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Eiben
authors.affiliation: Key Laboratory for Nano-Bio Interface, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Qiangbin
authors.initials: Q
authors.lastname: Wang
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D043942:Nanotubes; D014027:Tobacco Mosaic Virus / Q000737:chemistry
chemical_list: D036022:Capsid Proteins
keywords: chimerical scaffold; coassembly; disulfide bond; length-controllability; physical stabilization; tobacco mosaic virus