title: Enhanced enzymatic saccharification of sugarcane bagasse pretreated by combining O2 and NaOH.
abstract: Sugarcane bagasse pretreated by combining O2 and NaOH with different variables was conducted to improve its enzymatic digestibility and sugar recovery, and the results were compared with sole NaOH pretreatment. Lignin removal for O2-NaOH pretreatment was around 10% higher than that for sole NaOH pretreatment under the same conditions, and O2-NaOH pretreatment resulted in higher glucan recovery in the solid remain. Subsequently, O2-NaOH pretreated sugarcane bagasse presented more efficient enzymatic digestibility than sole NaOH pretreatment. Under the moderate pretreatment conditions of combining 1% NaOH and 0.5MPa O2 at 80°C for 120min, a high glucan conversion of 95% was achieved after 48h enzymatic hydrolysis. Coupled with the operations of pretreatment and enzymatic hydrolysis, an admirable total sugar recovery of 89% (glucose recovery of 93% and xylose recovery of 84%) was obtained. The susceptibility of the substrates to enzymatic digestibility was explained by their physical and chemical characteristics.
authors.affiliation: Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China.
authors.forename: Shuaizhu
authors.initials: S
authors.lastname: Bi
authors.affiliation: Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China. Electronic address: penglincai8@163.com.
authors.forename: Lincai
authors.initials: L
authors.lastname: Peng
authors.affiliation: Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China.
authors.forename: Keli
authors.initials: K
authors.lastname: Chen
authors.affiliation: Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China.
authors.forename: Zhengliang
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism; D012972:Sodium Hydroxide / Q000737:chemistry; D014994:Xylose
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D005936:Glucans; D012972:Sodium Hydroxide; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D014994:Xylose; D005947:Glucose; D010100:Oxygen
keywords: Delignification; Lignocellulosic biomass; O(2)–NaOH pretreatment; Sugar recovery