title: Developmental cigarette smoke exposure II: Kidney proteome profile alterations in 6 month old adult offspring.
abstract: Cigarette smoke exposure (CSE) during gestation and early development suppresses the growth trajectory in offspring. In prior studies utilizing a mouse model of 'active' developmental CSE (GD1-PD21), low birth weight induced by CSE persisted throughout the neonatal period and was present at the cessation of exposure at weaning with proportionally smaller kidney mass that was accompanied by impairment of carbohydrate metabolism. In the present study, littermates of those characterized in the prior study were maintained until 6 months of age at which time the impact of developmental CSE on the abundance of proteins associated with cellular metabolism in the kidney was examined. Kidney protein abundances were examined by 2D-SDS-PAGE based proteome profiling with statistical analysis by Partial Least Squares-Discriminant Analysis. Key findings of this study include a persistence of impact of developmental CSE past the original exposure period on the nucleic acid and carbohydrate metabolism networks and oxidant scavenging pathways.
authors.affiliation: Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, United States; Birth Defects Center, University of Louisville, Louisville, KY, United States. Electronic address: rachel.neal@louisville.edu.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Neal
authors.affiliation: Department of Molecular, Cellular, and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY, United States.
authors.forename: Rekha
authors.initials: R
authors.lastname: Jagadapillai
authors.affiliation: Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Molecular, Cellular, and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY, United States; Birth Defects Center, University of Louisville, Louisville, KY, United States.
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Webb
authors.affiliation: Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, United States.
authors.forename: Kendall
authors.initials: K
authors.lastname: Stocke
authors.affiliation: Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, United States.
authors.forename: Cailtin
authors.initials: C
authors.lastname: Gambrell
authors.affiliation: Department of Molecular, Cellular, and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY, United States; Birth Defects Center, University of Louisville, Louisville, KY, United States.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Greene
authors.affiliation: Department of Molecular, Cellular, and Craniofacial Biology, ULSD, University of Louisville, Louisville, KY, United States; Birth Defects Center, University of Louisville, Louisville, KY, United States.
authors.forename: M Michele
authors.initials: MM
authors.lastname: Pisano
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D018811:Maternal Exposure / Q000009:adverse effects; D008431:Maternal-Fetal Exchange; D008810:Mice, Inbred C57BL; D009696:Nucleic Acids / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced*; D020543:Proteome / Q000187:drug effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D009696:Nucleic Acids; D020543:Proteome; D014028:Tobacco Smoke Pollution
keywords: CSE; Developmental cigarette smoke exposure; Inhalation; Kidney; Mouse; Proteome; Tobacco