title: Overexpression of X chromosome-linked inhibitor of apoptosis by inhibiting microRNA-24 protects periodontal ligament cells against hydrogen peroxide-induced cell apoptosis.
abstract: Hydrogen peroxide (H2O2), a common oral clinical drug for the tooth bleaching, induces severe cell apoptosis of periodontal ligament cells (PDLCs). The excessive cell apoptosis of PDLCs impairs periodontal tissue damage and repair. However, the underlying mechanism is incompletely understood. Here, we showed that microRNA-24 (miR-24) played an important role in regulating H2O2-induced cell apoptosis of PDLCs. We found that miR-24 expression was increased in PDLCs in response to H2O2 treatment. Down-regulation of miR-24 obviously rescued H2O2-induced cell apoptosis in PDLCs. By bioinformatic analysis, X chromosome-linked inhibitor of apoptosis (XIAP) was identified as a candidate target gene of miR-24, which was further verified by the dual-luciferase reporter assay. Furthermore, the protein expression level of phosphatase and tensin homolog deleted on chromosome ten was significantly decreased by miR-24 silencing, whereas the phosphorylation of Akt was remarkably increased by miR-24 silencing. In addition, the gene silencing of XIAP significantly reduced Akt activity and blocked the protective effect of the miR-24 inhibitor against H2O2-induced cell apoptosis. Overall, our findings suggest that miR-24 plays an important role in regulating the cell survival of PDLCs through targeting XIAP.
authors.affiliation: Yan'an University Affiliated Hospital Department of Prosthodontics Yan'an City China caihongliuya@163.com.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Shaanxi Provincial People&apos;s Hospital Department of Prosthodontics Xi'an China.
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Yan'an University Affiliated Hospital Department of Prosthodontics Yan'an City China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Yan'an University Affiliated Hospital Department of Prosthodontics Yan'an City China.
authors.forename: H
authors.initials: H
authors.lastname: Hu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D019610:Cytoprotection / Q000187:drug effects*; D055785:Gene Knockdown Techniques; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology*; D020011:Protective Agents / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D020011:Protective Agents; D051636:X-Linked Inhibitor of Apoptosis Protein; D006861:Hydrogen Peroxide; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase