title: Immunoselection techniques in hematopoietic stem cell transplantation.
abstract: Hematopoietic Stem Cells Transplantation (HSCT) is an effective treatment for hematological and non-hematological diseases. The main challenge in autologous HSCT is purging of malignant cells to prevent relapse. In allogeneic HSCT graft-versus-host disease (GvHD) and opportunistic infections are frequent complications. Two types of graft manipulation have been introduced: the first one in the autologous context aimed at separating malignant cells from hematopoietic stem cells (HSC), and the second one in allogeneic HSCT aimed at reducing the incidence of GvHD and at accelerating immune reconstitution. Here we describe the manipulations used for cell purging in autologous HSCT or for T Cell Depletion (TCD) and T cell selection in allogeneic HSCT. More complex manipulations, requiring a Good Manufacturing Practice (GMP) facility, are briefly mentioned.
authors.affiliation: Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy. Electronic address: giuseppina.lipira@opbg.net.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Li Pira
authors.affiliation: Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Biagini
authors.affiliation: Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cicchetti
authors.affiliation: Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Merli
authors.affiliation: Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Letizia Pomponia
authors.initials: LP
authors.lastname: Brescia
authors.affiliation: Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Giuseppe Maria
authors.initials: GM
authors.lastname: Milano
authors.affiliation: Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Montanari
mesh_terms: D064591:Allografts; D064592:Autografts; D006086:Graft vs Host Disease / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008212:Lymphocyte Depletion / Q000379:methods*
keywords: Alloreactive cells; Cell manipulation; Graft-versus-host disease; Hematopoietic stem cell; Opportunistic infections