title: SH3-like motif-containing C-terminal domain of staphylococcal teichoic acid transporter suggests possible function.
abstract: The negatively charged bacterial polysaccharides-wall teichoic acids (WTAs) are synthesized intracellularly and exported by a two-component transporter, TagGH, comprising a transmembrane subunit TagG and an ATPase subunit TagH. We determined the crystal structure of the C-terminal domain of TagH (TagH-C) to investigate its function. The structure shows an N-terminal SH3-like subdomain wrapped by a C-terminal subdomain with an anti-parallel β-sheet and an outer shell of α-helices. A stretch of positively charged surface across the subdomain interface is flanked by two negatively charged regions, suggesting a potential binding site for negatively charged polymers, such as WTAs or acidic peptide chains. Proteins 2016; 84:1328-1332. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: Tzu-Ping
authors.initials: TP
authors.lastname: Ko
authors.affiliation: Department of Food and Nutrition, Providence University, Taichung City, Taiwan.|Department of Endocrinology and Metabolism, Kuang Tien General Hospital, Taiwan.
authors.forename: Shih-Ting
authors.initials: ST
authors.lastname: Tseng
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: Shu-Jung
authors.initials: SJ
authors.lastname: Lai
authors.affiliation: Department of Biotechnology, Hungkuang University, Taichung, Taiwan.
authors.forename: Sheng-Chia
authors.initials: SC
authors.lastname: Chen
authors.affiliation: Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu, 30076, Taiwan.
authors.forename: Hong-Hsiang
authors.initials: HH
authors.lastname: Guan
authors.affiliation: Department of Biotechnology, Hungkuang University, Taichung, Taiwan.|Taiwan Advance Biopharm (TABP) Inc, Xizhi City, New Taipei City, Taiwan.
authors.forename: Chia
authors.initials: C
authors.lastname: Shin Yang
authors.affiliation: Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu, 30076, Taiwan.|Institute of Biotechnology and Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan City, 701, Taiwan.|Department of Physics, National Tsing Hua University, Hsinchu, 30043, Taiwan.
authors.forename: Chun
authors.initials: C
authors.lastname: Jung Chen
authors.affiliation: Department of Biotechnology, Hungkuang University, Taichung, Taiwan.
authors.forename: Yeh
authors.initials: Y
authors.lastname: Chen
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport; D002473:Cell Wall / Q000737:chemistry; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000737:chemistry* / Q000201:enzymology; D055672:Static Electricity; D013682:Teichoic Acids / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D013682:Teichoic Acids; D006867:Hydrolases
keywords: ABC transporter; TagH; WTA; bacterial cell wall; crystal structure