title: Anti-inflammatory activity of polysaccharide from Schizophyllum commune as affected by ultrasonication.
abstract: Ultrasound treatment was applied to modify the physicochemical properties of an exopolysaccharide from mycelial culture of Schizophyllum commune. Molecular weight (MW) degradation, viscosity and anti-inflammatory property of ultrasonic treated polysaccharide were optimized with response surface methodology. The best ultrasonic parameters were obtained with a three-variable-three-level Box-Behnken design. The optimized conditions for efficient anti-inflammatory activity are initial concentration at 0.4%, ultrasonic power at 600W, and duration of ultrasonic irradiation for 9min. Under these conditions, the nitric oxide inhibition rate was 95±0.03% which agreed closely with the predicted value (96%). Average MW of polysaccharide decreased after ultrasonic treatments. The viscosity of degraded polysaccharide dropped compared with native polysaccharide. The anti-inflammatory activity was improved by ultrasound treatment. The results suggested that ultrasound treatment is an effective approach to decrease the MW of polysaccharide with high anti-inflammatory activity. Ultrasonic treatment is a viable modification technology for high MW polymer materials.
authors.affiliation: Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066600, China; School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China; Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong 519085, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong 519085, China.
authors.forename: Huansong
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066600, China.
authors.forename: Yuedong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Zhaoxiang
authors.initials: Z
authors.lastname: Bian
authors.affiliation: Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong 519085, China. Electronic address: baojunxu@uic.edu.hk.
authors.forename: Baojun
authors.initials: B
authors.lastname: Xu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000378:metabolism; D058105:Polymerization; D000067996:RAW 264.7 Cells; D012567:Schizophyllum / Q000737:chemistry*; D013010:Sonication; D013696:Temperature; D014783:Viscosity
chemical_list: D000893:Anti-Inflammatory Agents; D062610:Fungal Polysaccharides; D009569:Nitric Oxide
keywords: Anti-inflammatory; Response surface methodology; Ultrasonic treatment