title: Fermentation scale up for α-arbutin production by Xanthomonas BT-112.
abstract: α-Arbutin is a glycosylated hydroquinone that has an inhibitory function against tyrosinase. The aim of the present study is to develop an efficient and inexpensive method for large-scale production of α-arbutin by using Xanthomonas BT-112 as biocatalyst. To accomplish this goal, various surfactants were tested to enhance the α-arbutin production, and the optimal operational conditions for 30L jar fermenter were scaled up for a production level of 3000L with using a constant volumetric oxygen transfer coefficient (KLa) and the volumetric aeration rate per volume unit (Q/V) as scale-up criteria. Under the optimized conditions, the α-arbutin produced in the presence of 0.4% (w/v) Tween-80 was 124.8% higher than that of the control, and the yield of α-arbutin in 3000L fermenter was 38.2g/L with a molar conversion ratio of 93.7% based on the amount of hydroquinone supplied. This result is comparable to the results from laboratory-scale fermenter. Hence, 100-fold scale-up was successfully achieved.
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wei
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Changxia
authors.initials: C
authors.lastname: Liu
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Ruican
authors.initials: R
authors.lastname: Liu
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China; Amoy-BUCT Industrial of Bio-technovation Institute, Amoy, 361022, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China; Amoy-BUCT Industrial of Bio-technovation Institute, Amoy, 361022, China. Electronic address: liucq@mail.buct.edu.cn.
authors.forename: Chunqiao
authors.initials: C
authors.lastname: Liu
mesh_terms: D001104:Arbutin / Q000032:analysis* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D011136:Polysorbates; D013501:Surface-Active Agents; D014974:Xanthomonas / Q000378:metabolism*
chemical_list: D011136:Polysorbates; D013501:Surface-Active Agents; D001104:Arbutin
keywords: Fermentation; K(L)a; Scale-up; Surfactant; Xanthomonas; α-Arbutin