title: The effect of cannabinoids on dinitrofluorobenzene-induced experimental asthma in mice.
abstract: Cannabinoids have anti-inflammatory effects and can produce bronchodilation in the airways. We have investigated the effects of cannabinoids on tracheal hyperreactivity and airway inflammation in dinitrofluorobenzene (DNFB)-induced experimental non-atopic asthma in mice. 5-hydroxytryptamine (5-HT)-induced contraction response was enhanced while carbachol- and electrical field stimulation-induced contractions, and isoprenaline-induced relaxation responses were remained unchanged in DNFB group. The increased 5-HT-induced contractions were inhibited by incubation with either atropine or tetrodotoxin. DNFB application resulted in increased macrophage number in the bronchoalveolar lavage fluid (BALF). In vivo ACEA (CB1 agonist) treatment prevented the increase in 5-HT contractions, while JWH133 (CB2 agonist) had no effect. However, neither ACEA nor JWH133 prevented the increase in macrophage number in BALF. In vitro ACEA incubation also inhibited the increase in 5-HT contraction in DNFB group. These results show that cannabinoid CB1 receptor agonist can prevent tracheal hyperreactivity to 5-HT in DNFB-induced non-atopic asthma in mice.
authors.affiliation: Hacettepe University, Faculty of Pharmacy, Department of Pharmacology, Sihhiye, Ankara 06100, Turkey. Electronic address: turgutb@hacettepe.edu.tr.
authors.forename: Turgut Emrah
authors.initials: TE
authors.lastname: Bozkurt
authors.affiliation: Hacettepe University, Faculty of Pharmacy, Department of Pharmacology, Sihhiye, Ankara 06100, Turkey.
authors.forename: Yesim
authors.initials: Y
authors.lastname: Kaya
authors.affiliation: Hacettepe University, Faculty of Pharmacy, Department of Pharmacology, Sihhiye, Ankara 06100, Turkey.
authors.forename: Nezahat Tugba
authors.initials: NT
authors.lastname: Durlu-Kandilci
authors.affiliation: Hacettepe University, Faculty of Medicine, Department of Pathology, Sihhiye, Ankara 06100, Turkey.
authors.forename: Sevgen
authors.initials: S
authors.lastname: Onder
authors.affiliation: Hacettepe University, Faculty of Pharmacy, Department of Pharmacology, Sihhiye, Ankara 06100, Turkey.
authors.forename: Inci
authors.initials: I
authors.lastname: Sahin-Erdemli
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D001285:Atropine / Q000494:pharmacology; D063386:Cannabinoid Receptor Agonists / Q000494:pharmacology*; D002186:Cannabinoids / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D004139:Dinitrofluorobenzene; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D008264:Macrophages / Q000473:pathology / Q000502:physiology; D051379:Mice; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists* / Q000378:metabolism; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists* / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects* / Q000503:physiopathology
chemical_list: D018927:Anti-Asthmatic Agents; C578453:CNR1 protein, mouse; D063386:Cannabinoid Receptor Agonists; D002186:Cannabinoids; C571431:Cnr2 protein, mouse; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D012701:Serotonin; D013779:Tetrodotoxin; D001285:Atropine; D002217:Carbachol; D004139:Dinitrofluorobenzene; D000109:Acetylcholine; C432747:1,1-dimethylbutyl-1-deoxy-Delta(9)-THC
keywords: Airway hyperreactivity; Cannabinoids; DNFB; Mouse; Non-atopic asthma