title: Evaluation of the Interaction between the Poincianella pyramidalis (Tul.) LP Queiroz Extract and Antimicrobials Using Biological and Analytical Models.
abstract: Poincianella pyramidalis (Tul.) LP Queiroz (Fabaceae) is an endemic tree of northeastern Brazil, occurring mainly in the Caatinga. Its medicinal use is widespread and is an important therapeutic option against diarrhea, dysentery, and respiratory and urinary infections, among other diseases. In this study we determined the chemical marker and evaluated the interaction between P. pyramidalis extract and a commercial antimicrobial through the use of biological and analytical models. To obtain the extract, an ethanol-water mixture (50:50 v/v) was used as solvent. It was nebulized in a spray dryer using colloidal silicon dioxide as a drying adjuvant. The extract (ENPp) was subjected to HPLC analysis to verify the presence of certain secondary metabolites. The Minimum Inhibitory Concentration (MIC) of the extract against Gram-negative bacteria was determined by broth microdilution and the MIC of synthetic antimicrobial drugs in the presence and absence of the extract. The antioxidant activity of ENPp was evaluated by the DPPH method. The compatibility between the antimicrobial and the extract was evaluated by thermal analysis (TG/DTA). The acute toxicity of the extract was evaluated in vivo in rodents. The results indicate significant additive action of the extract on synthetic antibiotics, considerable antioxidant activity and absence of toxicity. This extract shows high potential for the development of formulations for antimicrobial therapy when used with a vegetable-active ingredient.
authors.affiliation: Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.|Department of Natural Sciences, Universidade Federal do Piauí, Bom Jesus, Piauí, Brazil.
authors.forename: Thiago P
authors.initials: TP
authors.lastname: Chaves
authors.affiliation: Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.|School of Pharmaceutical Sciences, Universidade Estadual Paulista, Araraquara, São Paulo, Brazil.
authors.forename: Felipe Hugo A
authors.initials: FH
authors.lastname: Fernandes
authors.affiliation: Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
authors.forename: Cleildo P
authors.initials: CP
authors.lastname: Santana
authors.affiliation: Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
authors.forename: Jocimar S
authors.initials: JS
authors.lastname: Santos
authors.affiliation: Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
authors.forename: Francinalva D
authors.initials: FD
authors.lastname: Medeiros
authors.affiliation: Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
authors.forename: Délcio C
authors.initials: DC
authors.lastname: Felismino
authors.affiliation: Laboratory of Pharmacology, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
authors.forename: Vanda L
authors.initials: VL
authors.lastname: Santos
authors.affiliation: Laboratory of Research in Microbiology, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
authors.forename: Raïssa Mayer R
authors.initials: RM
authors.lastname: Catão
authors.affiliation: Laboratory of Microbiology and Molecular Biology, Universidade Regional do Cariri, Crato, CE, Brazil.
authors.forename: Henrique Douglas M
authors.initials: HD
authors.lastname: Coutinho
authors.affiliation: Laboratory of drug assay and development, Universidade Estadual da Paraíba, Campina Grande, Paraíba, Brazil.
authors.forename: Ana Cláudia D
authors.identifier: 0000-0002-8682-0178
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D029911:Caesalpinia / Q000737:chemistry*; D002845:Chromatography; D004347:Drug Interactions; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D064209:Phytochemicals / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013816:Thermodynamics; D013818:Thermogravimetry; D023382:Toxicity Tests, Acute
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D005419:Flavonoids; D064209:Phytochemicals; D010936:Plant Extracts; D059808:Polyphenols