title: Down-regulation of ABCG2 and ABCB4 transporters in the placenta of rats exposed to cadmium.
abstract: As a maternal and developmental toxicant, cadmium (Cd) possesses weak penetrability through the placental barrier. However, the underlying mechanism remains unclear. To gain insight into the protein molecules associated with Cd toxicity in placenta and explore their roles in Cd transportation, a reproductive animal experiment was carried out using Sprague-Dawley rats. We performed proteomic analysis of the placenta by Difference Gel Electrophoresis (DIGE) combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Tandem Mass Spectroscopy (MALDI-TOF/TOF MS). The DIGE assay identified 15 protein spots that were differentially expressed with a greater than 1.5-fold change in placenta of Cd-treated rats compared to the control rats. Based on the expression patterns and biological functions of the proteins, we selected the ABCG2 and ABCB4 transporter proteins for further analysis. Western blot analysis showed that Cd exposure could down-regulate the expression of ABCG2 and ABCB4 in the placenta. There was a negative dose-response relationship between Cd exposure and the expression of ABCG2 or ABCB4 protein. These results indicated that down-regulation of ABCG2 and ABCB4 transporters may regulate Cd across through placenta and thus affect the in vivo toxic effect of Cd to fetus.
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou, China.|Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Pathophysiology, Southern Medical University, Guangzhou, China.
authors.forename: Shuiwang
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Shanyu
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Dong
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ding
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000096:biosynthesis*; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000096:biosynthesis*; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D015536:Down-Regulation; D005260:Female; D008297:Male; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D000097202:ATP-Binding Cassette Sub-Family B Member 4
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D002104:Cadmium; D000097202:ATP-Binding Cassette Sub-Family B Member 4; C489249:Abcg2 protein, rat
keywords: ABCB4; ABCG2; cadmium; placenta; proteomics