title: Transcriptional regulation of topology modulators and transcription regulators of Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis (Mtb) is a formidable pathogen which has the ability to survive the hostile environment of the host by evading the host defense system. The re-configuration of its transcriptional and metabolic process allows the pathogen to confront the adverse environment within the host macrophages. The factors that assist the transcription and modulate the DNA topology would have to play a key role in the regulation of global gene expression of the organism. How transcription of these essential housekeeping genes alters in response to growth conditions and environmental stress has not been addressed together in a set of experimental conditions in Mtb. Now, we have mapped the transcription start sites (TSS) and promoters of several genes that play a central role in the regulation of DNA topology and transcription in Mtb. Using in vivo reporter assays, we validated the activity of the identified promoter elements in different growth conditions. The variation in transcript abundance of these essential genes was also analyzed in growth phase-dependent manner. These data provide the first glimpse into the specific adaptive changes in the expression of genes involved in transcription and DNA topology modulation in Mtb.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Bhavna
authors.initials: B
authors.lastname: Padmanabhan
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Adwait Anand
authors.initials: AA
authors.lastname: Godbole
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Tare
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Wareed
authors.initials: W
authors.lastname: Ahmed
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Kommireddy
authors.initials: K
authors.lastname: Vasu
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Arnab
authors.initials: A
authors.lastname: China
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Rupesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Mitra
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India; Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, 560064, India. Electronic address: vraj@mcbl.iisc.ernet.in.
authors.forename: Valakunja
authors.initials: V
authors.lastname: Nagaraja
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins
keywords: Mycobacterium; Promoter; Topology; Transcription