title: Use of Reporter Genes in the Generation of Vaccinia Virus-Derived Vectors.
abstract: Vaccinia virus (VACV) is one of the most extensively-studied viruses of the Poxviridae family. It is easy to genetically modify, so it has become a key tool for many applications. In this context, reporter genes facilitate the study of the role of foreign genes introduced into the genome of VACV. In this review, we describe the type of reporter genes that have been used to generate reporter-expressing VACV and the applications of the recombinant viruses obtained. Reporter-expressing VACV are currently employed in basic and immunology research, in the development of vaccines and cancer treatment.
authors.forename: Sally
authors.initials: S
authors.lastname: Al Ali
authors.affiliation: Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma, E-28029 Madrid, Spain. sara.baldanta@uam.es.
authors.forename: Sara
authors.initials: S
authors.lastname: Baldanta
authors.affiliation: Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma, E-28029 Madrid, Spain. mercedes.fernandeze@uam.es.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Fernández-Escobar
authors.affiliation: Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma, E-28029 Madrid, Spain. susana.guerra@uam.es.
authors.forename: Susana
authors.initials: S
authors.lastname: Guerra
mesh_terms: D017930:Genes, Reporter; D005822:Genetic Vectors; D008967:Molecular Biology / Q000379:methods*; D014616:Vaccinia virus / Q000235:genetics*; D014773:Virology / Q000379:methods*
keywords: reporter-expressing virus; vaccine; vaccinia virus