title: Role of SULT-mediated sulfation in the biotransformation of 2-butoxyethanol and sorbitan monolaurate: A study using zebrafish SULTs.
abstract: 2-Butoxyethanol and sorbitan monolaurate are major components of oil dispersants that are applied in large quantities to control oil spill in the aquatic environment. An important question is whether aquatic animals are equipped with mechanisms for the detoxification of these oil dispersant compounds. The current study aimed to examine whether zebrafish cytosolic sulfotransferases (SULTs) are capable of sulfating 2-butoxyethanol and sorbitan monolaurate. A systematic analysis of 18 zebrafish SULTs revealed that SULT3 ST1 showed the strongest sulfating activity toward 2-butoxyethanol, while SULT1 ST3 displayed the strongest sulfating activity toward sorbitan monolaurate. The pH-dependence of these two SULTs in mediating the sulfation of 2-butoxyethanol or sorbitan monolaurate was examined. Taken together, these results implied that SULT-mediated sulfation may function in the detoxification of these two oil dispersant compounds.
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH 43614, USA.
authors.forename: Yuecheng
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH 43614, USA.
authors.forename: Helen
authors.initials: H
authors.lastname: Seyoum
authors.affiliation: Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo Health Science Campus, Toledo, OH 43614, USA. Electronic address: ming.liu@utoledo.edu.
authors.forename: Ming-Cheh
authors.initials: MC
authors.lastname: Liu
mesh_terms: D000818:Animals; D001711:Biotransformation; D003600:Cytosol / Q000201:enzymology; D005026:Ethylene Glycols / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D005026:Ethylene Glycols; D006601:Hexoses; D013431:Sulfates; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C014822:sorbitan monolaurate; D015238:Sulfotransferases; C017096:n-butoxyethanol
keywords: 2-Butoxyethanol; Cytosolic sulfotransferase; SULT; Sorbitan monolaurate; Sulfation; Zebrafish