title: High Prevalence and Properties of Enterotoxin-Producing Staphylococcus aureus ST5 Strains of Food Sources in China.
abstract: Staphylococcus aureus with the ability of staphylococcal enterotoxins (SEs) production is one of the most common causes of bacterial foodborne outbreaks worldwide. In our study, 336 S. aureus isolates were recovered from 3476 food samples during 2010-2014. A total of 86 S. aureus isolates were proved to be enterotoxin-producing strains with PCR and enzyme-linked immunosorbent assay. In the 86 isolates, 20 STs were identified using multilocus sequence typing (MLST) and 20 isolates were typed as sequence type 5 (ST5), which was the most prevalent ST using MLST. There were six SE profiles and high carrier rates of sec (50%) and sed (75%) genes in the 20 S. aureus ST5 isolates. Additionally, 8 antibiotic resistance patterns were observed, and 10 multidrug-resistant isolates (50%) and 4 methicillin-resistant S. aureus isolates were identified. Our findings illustrate high prevalence of S. aureus ST5 isolates from food sources and diversity in SE profiles and antibiotic resistance patterns. These results indicate that great difference in the ability of obtaining SE production and antimicrobial resistance may exist between different genetic lineages of S. aureus strains.
authors.affiliation: 1 Ningbo Medical Treatment Center Lihuili Hospital , Ningbo, China .
authors.forename: Yanzi
authors.initials: Y
authors.lastname: Chang
authors.affiliation: 2 Department of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention , Ningbo, China .
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.affiliation: 3 Xiangshan Entry-Exit Inspection and Quarantine Bureau , Ningbo, China .
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: 2 Department of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention , Ningbo, China .
authors.forename: Shuo
authors.initials: S
authors.lastname: Ye
authors.affiliation: 2 Department of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention , Ningbo, China .
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: 2 Department of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention , Ningbo, China .
authors.forename: Xuanyi
authors.initials: X
authors.lastname: Shen
authors.affiliation: 2 Department of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention , Ningbo, China .
authors.forename: Danyang
authors.initials: D
authors.lastname: Zhang
authors.affiliation: 2 Department of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention , Ningbo, China .
authors.forename: Qifa
authors.initials: Q
authors.lastname: Song
mesh_terms: D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000737:chemistry*; D005516:Food Microbiology; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins; D037521:Virulence Factors