title: Mesenchymal stem cells promote pancreatic adenocarcinoma cells invasion by transforming growth factor-β1 induced epithelial-mesenchymal transition.
abstract: Mesenchymal stem cells (MSCs) could be ideal delivery vehicles for antitumor biological agents in pancreatic adenocarcinoma (PA). While the role of MSCs in tumor growth is elusive. Inflammation is an important feature of PA. In this study, we reported that MSCs pre-stimulated with the combination of TNF-α and IFN-γ promote PA cells invasion. The invasion of PA cell lines were evaluate by wound healing assay and transwell assay in vitro and liver metastasis in nude mice. We observed MSCs pre-stimulated with the combination of TNF-α and IFN-γ promoted PA cells invasion in vitro and in vivo. Consistent with MSCs promoting PA cells invasion, PA cells were found undergo epithelial-mesenchymal transition (EMT). We demonstrated that MSCs pre-stimulated with both of TNF-α and IFN-γ provoked expression transforming growth factor-β1 (TGF-β1). MSCs promoting EMT-mediated PA cells invasion could be reversed by short interfering RNA of TGF-β1. Our results suggest that MSCs could promote PA cells invasion in inflammation microenvironment and should be cautious as delivery vehicles in molecular target therapy.
authors.affiliation: Nanjing Lishui People's Hospital, Nanjing 211200, P.R. China.
authors.forename: Hai-Sen
authors.initials: HS
authors.lastname: Zhou
authors.affiliation: Department of Rehabilitation Medicine, The 101st Hospital of Chinese PLA, Wuxi 214044, P.R. China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Su
authors.affiliation: Health Science Center, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Xing-Li
authors.initials: XL
authors.lastname: Fu
authors.affiliation: Department of General Surgery, The 101st Hospital of Chinese PLA, Wuxi 214044, P.R. China.
authors.forename: Guo-Zhong
authors.initials: GZ
authors.lastname: Wu
authors.affiliation: Department of General Surgery, The 101st Hospital of Chinese PLA, Wuxi 214044, P.R. China.
authors.forename: Kun-Lun
authors.initials: KL
authors.lastname: Luo
authors.affiliation: Department of General Surgery, The 101st Hospital of Chinese PLA, Wuxi 214044, P.R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Department of General Surgery, The 101st Hospital of Chinese PLA, Wuxi 214044, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of General Surgery, The 101st Hospital of Chinese PLA, Wuxi 214044, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Nanjing Lishui People's Hospital, Nanjing 211200, P.R. China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Hu
authors.affiliation: Nanjing Lishui People's Hospital, Nanjing 211200, P.R. China.
authors.forename: Liu-Sheng
authors.initials: LS
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Wuxi People's Hospital, Nanjing Medical University, Wuxi 214023, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
authors.affiliation: Department of General Surgery, Wuxi People's Hospital, Nanjing Medical University, Wuxi 214023, P.R. China.|Department of General Surgery, The 101st Hospital of Chinese PLA, Wuxi 214044, P.R. China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Tian
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism / Q000494:pharmacology*; D059016:Tumor Microenvironment / Q000187:drug effects
chemical_list: D053773:Transforming Growth Factor beta1
keywords: epithelial-mesenchymal transition; mesenchymal stem cells; pancreatic adenocarcinoma; transforming growth factor-β1