title: The Autographa californica multiple nucleopolyhedrovirus ac110 gene encodes a new per os infectivity factor.
abstract: The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac110 gene is especially conserved in lepidopteran-specific baculoviruses and is uncharacterized. To investigate the role of ac110 in the baculovirus life cycle, an ac110-knockout (vAc110KO) and a repair (vAc110:HA) viruses were constructed in this study. Budded virion production and occlusion body formation were unaffected in vAc110KO-transfected or infected Sf9 cells. Intrahemocoelic injection of budded virions of vAc110KO killed Trichoplusia ni larvae as efficiently as the repair or the wild-type viruses. However, per os inoculation of occlusion bodies of vAc110KO failed to establish infection in T. ni larvae, while the repair virus was as efficient as the wild-type virus. Treatment with calcofluor white, a reagent that damages the peritrophic membrane, did not rescue the oral infectivity of vAc110KO. These results suggested that Ac110 is a new per os infectivity factor that may play a role after occlusion-derived virions pass through the peritrophic membrane during oral infection.
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China; School of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China.
authors.lastname: Jiantao Liu
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Leyuan
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Meijin
authors.initials: M
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: wuwenbi3@mail.sysu.edu.cn.
authors.forename: Wenbi
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: yangkai@mail.sysu.edu.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
mesh_terms: D000818:Animals; D055786:Gene Knockout Techniques; D005814:Genes, Viral; D005816:Genetic Complementation Test; D007814:Larva / Q000502:physiology / Q000821:virology; D007915:Lepidoptera; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology*; D061987:Sf9 Cells; D016019:Survival Analysis; D014774:Virulence
keywords: AcMNPV; Baculovirus; Peritrophic membrane; ac110; per os infectivity factor