title: Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp. cucumerinum.
abstract: Actinomycetes have received considerable attention as biocontrol agents against fungal plant pathogens and as plant growth promoters. In this study, a total of 320 actinomycetes were isolated from various habitats in China. Among which, 77 strains have been identified as antagonistic activities against Fusarium oxysporum f. sp. cucumerinum which usually caused fusarium wilt of cucumber. Of these, isolate actinomycete M527 not only displayed broad-spectrum antifungal activity but also showed the strongest antagonistic activity against the spore germination of F. oxysporum f. sp. cucumerinum. In pot experiments, the results indicated that isolate M527 could promote the shoot growth and prevent the development of the disease on cucumber caused by F. oxysporum f. sp. cucumerinum. The control efficacy against seedling fusarium wilt of cucumber after M527 fermentation broth root-irrigation was up to 72.1% as compared to control. Based on 16S rDNA sequence analysis, the isolate M527 was identified as Streptomyces rimosus.
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Lu
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, China.
authors.forename: Xianhao
authors.initials: X
authors.lastname: Xu
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yu
mesh_terms: D000935:Antifungal Agents; D061046:Biological Control Agents; D018553:Cucumis sativus / Q000382:microbiology*; D005670:Fusarium / Q000254:growth & development*; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D018520:Plant Shoots / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D036226:Seedlings / Q000382:microbiology; D013172:Spores, Fungal / Q000254:growth & development; D064411:Streptomyces rimosus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D061046:Biological Control Agents; D012336:RNA, Ribosomal, 16S
keywords: Antagonistic actinomycetes; Biocontrol; Fusarium oxysporum f. sp. Cucumerinum; Streptomyces rimosus