title: Metarhizium anisopliae for controlling Rhipicephalus microplus ticks under field conditions.
abstract: Metarril SP Organic is a product based on the fungus Metarhizium anisopliae, which was developed for controlling agricultural pests. The present study evaluated the effect of Metarril SP Organic plus 10% mineral oil, for controlling Rhipicephalus microplus under field conditions. Three groups were formed: Control group, which received no treatment; Oil control group, which was bathed only with water, Tween 80 and mineral oil; and Metarril group, bathed in the oil-based formulation Metarril SP Organic. Two treatments per group were performed and to verify the effect of the treatments, all R. microplus ticks between 4.5 and 8.0mm in length on the left side of the cattle were counted on days +7, +14 and +21 after each treatment, and a sample of engorged females was collected for evaluation of biological parameters. The Metarril SP Organic oil formulation showed efficacy ranging from 8.53 to 90.53%. The average efficacy of the oil-based formulation of Metarril SP Organic was 75.09 and 46.59% compared with the groups Control and Oil control, respectively. There were no significant changes in biological parameters of engorged R. microplus females collected from animals. Although there was no significant difference in the amount of ticks between the Oil control and Metarril groups, it is believed that the association of mineral oil with Metarril SP Organic product is effective in R. microplus tick control in field. Thus, this association has potential to be used in strategic control programs of cattle tick.
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil.
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Camargo
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil.
authors.forename: Michel R S
authors.initials: MR
authors.lastname: Nogueira
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil.
authors.forename: Allan F
authors.initials: AF
authors.lastname: Marciano
authors.affiliation: Departamento de Parasitologia, Faculdade de Medicina Veterinária, Universidade de Cuiabá, Avenida Beira Rio, Jardim Europa, 3100, Cuiabá, Mato Grosso, CEP 78015-480, Brazil.
authors.forename: Wendell M S
authors.initials: WM
authors.lastname: Perinotto
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil.
authors.forename: Caio J B
authors.initials: CJ
authors.lastname: Coutinho-Rodrigues
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil.
authors.forename: Fábio B
authors.initials: FB
authors.lastname: Scott
authors.affiliation: Departamento de Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil.
authors.forename: Isabele C
authors.initials: IC
authors.lastname: Angelo
authors.affiliation: Embrapa Gado de Leite, Rua Eugênio do Nascimento, 610, Bairro Dom Bosco, Juiz de Fora, Minas Gerais, CEP 36038-330, Brazil.
authors.forename: Márcia C A
authors.initials: MC
authors.lastname: Prata
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil. Electronic address: vaniabit@ufrrj.br.
authors.forename: Vânia R E P
authors.initials: VR
authors.lastname: Bittencourt
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology* / Q000628:therapy; D052981:Metarhizium / Q000502:physiology*; D008899:Mineral Oil / Q000494:pharmacology; D010572:Pest Control, Biological; D048494:Rhipicephalus / Q000187:drug effects / Q000382:microbiology*; D013983:Tick Control / Q000379:methods*
chemical_list: D008899:Mineral Oil
keywords: Arthropod-pathogenic fungi; Biological control; Cattle tick; Oil-based formulations