title: Impact of tightly focused femtosecond laser pulses on nucleolus-like bodies of mouse GV oocyte and the ability of mouse oocytes to mature.
abstract: Using femtosecond laser radiation, nucleolus-like bodies (NLBs) of mouse oocytes were locally dissected without damage to zona pellucida, cytoplasmic membrane, nuclear membrane, and nucleoplasm surrounding NLB. It was found that, after dissection of 2.7 × 10(-11) cm(3) of NLB material, which is approximately 5.2% of 10 μm NLB volume, the probability of germinal vesicle oocyte development to metaphase II stage of meiosis decreased 3-7 times compared to the non-treated oocytes. This result indicates that NLB material organization is significant for mouse oocyte maturation.
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Astafev
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Zalesskiy
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Zatsepina
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Kostrov
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Krivoharchenko
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Osychenko
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Serobyan
authors.affiliation: Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia. nadtochenko@gmail.com.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia. nadtochenko@gmail.com.|Moscow State University, Moscow, Russia. nadtochenko@gmail.com.
authors.forename: V A
authors.initials: VA
authors.lastname: Nadtochenko
mesh_terms: D000818:Animals; D004867:Equipment Design; D007834:Lasers; D008540:Meiosis / Q000502:physiology / Q000528:radiation effects; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D009866:Oogenesis / Q000502:physiology / Q000528:radiation effects*; D013997:Time Factors