title: The inhibitors of Arp2/3 complex and WASP proteins modulate the effect of glutoxim on Na(+) transport in frog skin.
abstract: Using voltage-clamp technique, the involvement of WASP proteins and Arp2/3 complex in the effect of immunomodulator drug glutoxim on Na(+) transport in frog skin was investigated. It was shown for the first time that preincubation of the skin with the N-WASP inhibitor wiskostatin or the Arp2/3 complex inhibitor CK-0944666 significantly decreases the stimulatory effect of glutoxim on Na(+) transport. The data suggest the involvement of actin filament polymerization and branching in the glutoxim effect on Na(+) transport in frog skin.
authors.affiliation: St. Petersburg State University, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia. zk@bio.spbu.ru.
authors.forename: Z I
authors.initials: ZI
authors.lastname: Krutetskaya
authors.affiliation: St. Petersburg State University, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Melnitskaya
authors.affiliation: St. Petersburg State University, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Antonov
authors.affiliation: St. Petersburg State University, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Nozdrachev
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D003879:Dermatologic Agents / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D011412:Propanolamines / Q000494:pharmacology; D011896:Rana temporaria; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D051316:Wiskott-Aldrich Syndrome Protein / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D051376:Actin-Related Protein 2-3 Complex; C543733:CK-0944666; D002227:Carbazoles; D003879:Dermatologic Agents; D007211:Indoles; D009842:Oligopeptides; D011412:Propanolamines; D051316:Wiskott-Aldrich Syndrome Protein; C462699:glutoxim; C489942:wiskostatin; D012964:Sodium