title: Development and validation of an HILIC-MS/MS method by one-step precipitation for chloroquine in miniature pig plasma.
abstract: BACKGROUND
  Quantification of polar compounds such as chloroquine by revered-phase LC is a challenge because of poor retention and silanol interactions with stationary phase. Strong ion-pairing reagents added to mobile phases to improve reversed-phase retention and improve peak shape can be harmful for MS.
  
  
  RESULTS
  This new approach provides a rapid and sensitive method for the detection of chloroquine using hydrophilic interaction LC coupled to MS/MS (HILIC-MS/MS). Ammonium formate and formic acid were added to mobile phase to attain good peak shapes and the salified chloroquine as well retained in an HILIC column. Linearity, intra- and inter-day precision, accuracy, recovery, matrix effect and stability were evaluated during the validation process.
  
  
  CONCLUSIONS
  The validated method has been successfully used in a PK study in miniature pigs, and paves way for future development.
authors.affiliation: The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou 510370, China.
authors.forename: Zhan-Zhang
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou 510370, China.
authors.forename: Hao-Yang
authors.initials: HY
authors.lastname: Lu
authors.affiliation: The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou 510370, China.
authors.forename: De-Wei
authors.initials: DW
authors.lastname: Shang
authors.affiliation: The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou 510370, China.
authors.forename: Xiao-Jia
authors.initials: XJ
authors.lastname: Ni
authors.affiliation: The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou 510370, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou 510370, China.
authors.forename: Yu-Guan
authors.initials: YG
authors.lastname: Wen
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000097:blood*; D002738:Chloroquine / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D057230:Limit of Detection; D013552:Swine; D013556:Swine, Miniature / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000962:Antimalarials; D002738:Chloroquine
keywords: HILIC; LC–MS/MS; chloroquine