title: Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp. hordei.
abstract: Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a 'Lenetah' × 'Grannelose Zweizeilige' (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a '95SR316A' × GZ cross for their ability to predict seedling resistance. In 95SR316A × GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ.
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: K
authors.initials: K
authors.lastname: Esvelt Klos
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: T
authors.initials: T
authors.lastname: Gordon
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: P
authors.initials: P
authors.lastname: Bregitzer
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: P
authors.initials: P
authors.lastname: Hayes
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: X M
authors.initials: XM
authors.lastname: Chen
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: I A
authors.initials: IA
authors.lastname: Del Blanco
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: S
authors.initials: S
authors.lastname: Fisk
authors.affiliation: First, second, third, and eighth authors: Agricultural Research Service, United States Department of Agriculture (USDA), Aberdeen, ID 83210; fourth and seventh authors: Department of Crop and Soil Science, Oregon State University, Corvallis 97331; fifth author: USDA Agricultural Research Service, Pullman, WA 99164; and sixth author: Department of Plant Sciences, University of California, Davis 95616.
authors.forename: J M
authors.initials: JM
authors.lastname: Bonman
mesh_terms: D000483:Alleles; D001487:Basidiomycota / Q000502:physiology*; D060467:Disease Resistance / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D060005:Genotyping Techniques; D001467:Hordeum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D036226:Seedlings / Q000235:genetics / Q000276:immunology / Q000382:microbiology
chemical_list: D005819:Genetic Markers