title: New anti-inflammatory sterols from a gorgonian Pinnigorgia sp.
abstract: Chemical investigation on the EtOAc-soluble fraction from the MeOH/DCM extract of a gorgonian Pinnigorgia sp. afforded two new sterols, 11-acetoxy-24S-methyl-3β,5α,6α-trihydroxy-9,11-secocholest-7-en-9-one (1) and 5β,6β-epoxy-(22E,24R)-ergosta-8,22-diene-3β,7β-diol (2). The structures of sterols 1 and 2 were elucidated on the basis of spectroscopic analysis and by comparison of their spectroscopic data with those of related analogues. Both 1 and 2 were shown to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.
authors.forename: Yin-Di
authors.initials: YD
authors.lastname: Su
authors.affiliation: Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, Taiwan.
authors.forename: Ching-Hsiao
authors.initials: CH
authors.lastname: Cheng
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan; Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: yachwu@mail.cmu.edu.tw.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. Electronic address: pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D012632:Secosteroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C000621107:11-acetoxy-24-methyl-3,5,6-trihydroxy-9,11-secocholest-7-en-9-one; C000621108:5,6-epoxyergosta-8,22-diene-3,7-diol; D000893:Anti-Inflammatory Agents; D052246:Cyclooxygenase 2 Inhibitors; D008070:Lipopolysaccharides; D012632:Secosteroids; D013261:Sterols; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D004875:Ergosterol
keywords: Anti-inflammatory activity; COX-2; Gorgonian; Pinnigorgia; Sterol; iNOS