title: Proteomic analysis of skeletal deformity in diploid and triploid rainbow trout (Oncorhynchus mykiss) larvae.
abstract: A proteomic screening approach was employed to achieve a better understanding of the changes that occur in protein expression patterns associated with skeletal deformities in both diploid and triploid rainbow trout larvae. Triploidy was induced through the application of heat shock of 28°C for 10min to eggs 10-min post fertilization in an aquarium equipped with a heater. Percentage of skeletal deformity in heat-shocked larvae (2.88±0.30, mean±S.E.) was significantly (P<0.05) greater than that of the diploids (0.55±0.24). At five days after hatching, proteins of normal and deformed specimens of deyolked larvae were subjected to proteomic analysis using two-dimensional electrophoresis and mass spectrometry. Among the identified protein spots from diploids, creatine kinase was found to be increased in larvae with skeletal deformities, while apolipoprotein A-I-2, apolipoprotein A-II and calmodulin were found to be decreased in deformed fish. Among the five protein spots that were identified in heat-shocked fish, apolipoprotein A-I-2, apolipoprotein A-II, parvalbumin, myosin light chain 1-1 and nucleoside diphosphate kinase were found to be decreased in deformed larvae. The identification of nine protein spots showing altered expression in deformed fish allows us to reach a preliminary view of the molecular mechanisms that are involved in the development of skeletal malformations in diploid and triploid fish.
authors.affiliation: Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran.
authors.forename: Samad Bahrami
authors.initials: SB
authors.lastname: Babaheydari
authors.affiliation: Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran. Electronic address: Keyvan56@yahoo.com.
authors.forename: Saeed
authors.initials: S
authors.lastname: Keyvanshokooh
authors.affiliation: Department of Natural Resources, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Salar
authors.initials: S
authors.lastname: Dorafshan
authors.affiliation: Department of Fisheries, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Kurdistan, Iran.
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Johari
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000002:abnormalities / Q000378:metabolism*; D004171:Diploidy; D015180:Electrophoresis, Gel, Two-Dimensional; D029941:Fish Proteins / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000002:abnormalities / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057885:Triploidy
chemical_list: D029941:Fish Proteins; D020543:Proteome
keywords: Diploid; Proteomics; Rainbow trout; Skeletal deformity; Triploid