title: Isolation and Structure Elucidation, Molecular Docking Studies of Screlotiumol from Soil Borne Fungi Screlotium rolfsii and their Reversal of Multidrug Resistance in Mouse Lymphoma Cells.
abstract: A new compound namely (13-(3,3-dihydroxypropyl)-1,6-dihydroxy-3,4-dihydro-1H-isochromen-8(5H)-one (1) was isolated from an ethyl acetate extract of the borne fungi Screlotium rolfsii. Its chemical structure was elucidated by spectroscopic analysis. Screlotiumol 1 were evaluated for their effects on the reversion of multidrug resistant (MDR) mediated by P-glycoprotein (P-gp) of the soil borne fungi. The multidrug resistant P-glycoprotein is a target for chemotherapeutic drugs in cancer cells. In the present study rhodamine-123 exclusion screening test on human mdr1 gene transfected mouse gene transfected L5178 and L5178Y mouse T-cell lymphoma which showed excellent MDR reversing effect in a dose dependent manner against mouse T-lymphoma cell line. Moreover, molecular docking studies of compound-1 also showed better results as compared with the standard. Therefore the preliminary results obtained from this study suggest that screlotiumol 1 could be used as a potential agent for the treatment of cancer.
authors.affiliation: Center of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan E-mail : mashaljcs@yahoo.com.
authors.forename: Bashir
authors.initials: B
authors.lastname: Ahmad
authors.forename: Muhammad
authors.initials: M
authors.lastname: Rizwan
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.forename: Muslim
authors.initials: M
authors.lastname: Raza
authors.forename: Sadiq
authors.initials: S
authors.lastname: Azam
authors.forename: Shumaila
authors.initials: S
authors.lastname: Bashir
authors.forename: Joseph
authors.initials: J
authors.lastname: Molnar
authors.forename: Akos
authors.initials: A
authors.lastname: Csonka
authors.forename: Diana
authors.initials: D
authors.lastname: Szabo
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005658:Fungi / Q000737:chemistry*; D006801:Humans; D016399:Lymphoma, T-Cell / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D062105:Molecular Docking Simulation; D010936:Plant Extracts / Q000494:pharmacology*; D012988:Soil Microbiology; D014407:Tumor Cells, Cultured
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D000085:Acetates; D000970:Antineoplastic Agents; D001578:Benzopyrans; D010936:Plant Extracts; C000613753:screlotiumol; C007650:ethyl acetate