title: Effects of Pamidronate on Dental Enamel Formation Assessed by Light Microscopy, Energy-Dispersive X-Ray Analysis, Scanning Electron Microscopy, and Microhardness Testing.
abstract: The aim of the present work was to investigate birefringence and morphology of the secretory-stage enamel organic extracellular matrix (EOECM), and structural and mechanical properties of mature enamel of upper incisors from adult rats that had been treated with pamidronate disodium (0.5 mg/kg/week for 56 days), using transmitted polarizing and bright-field light microscopies (TPLM and BFLM), energy-dispersive X-ray (EDX) analysis, scanning electron microscopy (SEM) and microhardness testing. BFLM showed no morphological changes of the EOECM in pamidronate and control groups, but TPLM revealed a statistically significant reduction in optical retardation values of birefringence brightness of pamidronate-treated rats when compared with control animals (p0.05). The present study indicates that pamidronate can affect birefringence of the secretory-stage EOECM, which does not seem to be associated with significant changes in morphological and/or mechanical properties of mature enamel.
authors.affiliation: 1Multidisciplinary Institute of Health,Federal University of Bahia-UFBA,Rua Rio de Contas,58,Quadra 17,Lote 58,Candeias,Vitória da Conquista,BACEP 45.029-094,Brasil.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Soares
authors.affiliation: 2Faculty of Pharmacy,Federal University of Bahia-UFBA,Rua Barão de Jeremoabo,S/N,Campus Universitário de Ondina,Ondina,Salvador,BA CEP 40.170-115,Brasil.
authors.forename: Renan F
authors.initials: RF
authors.lastname: do Espírito Santo
authors.affiliation: 3Piracicaba Dental School,University of Campinas-UNICAMP,Av. Limeira,901,Areião,Piracicaba,SP CEP 13.414-903,Brasil.
authors.forename: Sérgio R P
authors.initials: SR
authors.lastname: Line
authors.affiliation: 4School of Veterinary Medicine and Zootechny,Federal University of Bahia-UFBA,Av. Ademar de Barros,500,Ondina,Salvador,BA CEP 40.170-110,Brasil.
authors.forename: Maria das G F
authors.initials: Md
authors.lastname: Pinto
authors.affiliation: 5Professor Edgard Santos University Hospital,Federal University of Bahia-UFBA,Rua Augusto Viana,S/N,Canela,Salvador,BA CEP 40.110-060,Brasil.
authors.forename: Pablo de M
authors.initials: Pde M
authors.lastname: Santos
authors.affiliation: 6Institute of Health Sciences,Federal University of Bahia-UFBA,Av. Reitor Miguel Calmon,S/N,Vale do Canela,Salvador,BA CEP 40.110-902,Brasil.
authors.forename: Maria Betania P
authors.initials: MB
authors.lastname: Toralles
authors.affiliation: 6Institute of Health Sciences,Federal University of Bahia-UFBA,Av. Reitor Miguel Calmon,S/N,Vale do Canela,Salvador,BA CEP 40.110-902,Brasil.
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: do Espírito Santo
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D003743:Dental Enamel / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure*; D004164:Diphosphonates / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D000077268:Pamidronate; D051381:Rats; D014965:X-Rays
chemical_list: D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D000077268:Pamidronate
keywords: bisphosphonates; enamel; light microscopy; microanalysis; scanning electron microscopy