title: Isolation of Madre de Dios Virus (Orthobunyavirus; Bunyaviridae), an Oropouche Virus Species Reassortant, from a Monkey in Venezuela.
abstract: Oropouche virus (OROV), genus Orthobunyavirus, family Bunyaviridae, is an important cause of human illness in tropical South America. Herein, we report the isolation, complete genome sequence, genetic characterization, and phylogenetic analysis of an OROV species reassortant, Madre de Dios virus (MDDV), obtained from a sick monkey (Cebus olivaceus Schomburgk) collected in a forest near Atapirire, a small rural village located in Anzoategui State, Venezuela. MDDV is one of a growing number of naturally occurring OROV species reassortants isolated in South America and was known previously only from southern Peru.
authors.affiliation: Lab Biología de Vectores, Instituto de Zoología y Ecología Tropical, Universidad Central de Venezuela, Caracas, Venezuela. Universidad Internacional SEK, Quito, Ecuador.
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: Navarro
authors.affiliation: Lab Biología de Vectores, Instituto de Zoología y Ecología Tropical, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Dileyvic
authors.initials: D
authors.lastname: Giambalvo
authors.affiliation: Instituto Nacional de Higiene "Rafael Rangel" (INHRR), Ciudad Universitaria, Caracas, Venezuela.
authors.forename: Rosa
authors.initials: R
authors.lastname: Hernandez
authors.affiliation: Department of Microbiology and Immunology, Institute for Human Infections and Immunity, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Albert J
authors.initials: AJ
authors.lastname: Auguste
authors.affiliation: Department of Microbiology and Immunology, Institute for Human Infections and Immunity, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.affiliation: Department of Microbiology and Immunology, Institute for Human Infections and Immunity, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Weaver
authors.affiliation: Dirección General de Salud Ambiental, Ministerio del Poder Popular para la Salud, Caracas, Venezuela.
authors.forename: Humberto
authors.initials: H
authors.lastname: Montañez
authors.affiliation: Departamento de Biología, Facultad Experimental de Ciencias y Tecnología (FACYT), Universidad de Carabobo, Valencia, Venezuela.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Liria
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Lima
authors.affiliation: Departament of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: Jorge Fernando Soares
authors.initials: JF
authors.lastname: Travassos da Rosa
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: Sandro P
authors.initials: SP
authors.lastname: da Silva
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: Janaina M
authors.initials: JM
authors.lastname: Vasconcelos
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Oliveira
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
authors.forename: João L S G
authors.initials: JL
authors.lastname: Vianez
authors.affiliation: Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil. marcionunes@iec.pa.gov.br.
authors.forename: Marcio R T
authors.initials: MR
authors.lastname: Nunes
mesh_terms: D000818:Animals; D002044:Bunyaviridae Infections / Q000175:diagnosis / Q000821:virology; D002428:Cebus / Q000821:virology*; D002522:Chlorocebus aethiops; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D016851:Orthobunyavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014687:Venezuela; D014709:Vero Cells
chemical_list: D012367:RNA, Viral