title: Enhanced cadmium efflux and root-to-shoot translocation are conserved in the hyperaccumulator Sedum alfredii (Crassulaceae family).
abstract: Investigation on the molecular mechanisms of cadmium hyperaccumulation has been mostly focused on members of the Brassicaceae family. Here, we show using hyperaccumulating (HP) and nonhyperaccumulating (NHP) populations of Sedum alfredii (Crassulaceae), that Cd hypertolerance correlates with higher Cd efflux rates and less cadmium accumulation in suspension cells and roots. The heavy metal ATPase HMA2, but not HMA4, was highly expressed in suspension cultures and roots from HP plants compared to NHP cells and plants. Reciprocal grafting also showed that Cd translocation is more efficient in HP plants. These results suggest that cadmium efflux is a conserved mechanism among natural cadmium hyperaccumulator species.
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, China.
authors.forename: Zhongchun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yu
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, China.
authors.forename: Hanying
authors.initials: H
authors.lastname: Du
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Ai
authors.affiliation: College of Plant Science and Technology, Huazhong Agriculture University, Wuhan, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Yao
authors.affiliation: Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.
authors.forename: David G
authors.initials: DG
authors.lastname: Mendoza-Cózatl
authors.affiliation: School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, China.
authors.forename: Baosheng
authors.initials: B
authors.lastname: Qiu
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology; D002104:Cadmium / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism*; D031963:Sedum / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D002104:Cadmium; D000251:Adenosine Triphosphatases
keywords: cadmium efflux; cadmium hyperaccumulation; cadmium hypertolerance