title: A dual-channel detection of mercuric ions using a label free G-quadruplex-based DNAzyme molecule.
abstract: We have constructed a 'turn-off' and label free bio-sensor using a DNAzyme molecule. This facile bio-sensor is capable of selective detection of mercuric ions with a high sensitivity and satisfactory dynamic range. More importantly, it is able to generate both fluorescent and colourimetric signals for detection. This dual-channel bio-sensor is expected to afford high detection confidence and overcome false-positive readout especially when assaying complex biological samples.
authors.affiliation: Biomolecular Sciences Research Complex, EaStCHEM School of Chemistry, University of St Andrews, Fife KY16 9ST, UK. lm228@st.andrews.ac.uk and Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Guanrong
authors.initials: G
authors.lastname: Wu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Sun
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Lingpei
authors.initials: L
authors.lastname: Meng
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Yuyin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. malong@tust.edu.cn and Tianjin Key Laboratory of Industry Microbiology, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
authors.forename: Aipo
authors.initials: A
authors.lastname: Diao
mesh_terms: D015374:Biosensing Techniques; D021881:DNA, Catalytic / Q000737:chemistry*; D054856:G-Quadruplexes; D007477:Ions; D008628:Mercury / Q000032:analysis*
chemical_list: D021881:DNA, Catalytic; D007477:Ions; D008628:Mercury