title: Transcriptomic effects of the non-steroidal anti-inflammatory drug Ibuprofen in the marine bivalve Mytilus galloprovincialis Lam.
abstract: The transcriptomic effects of Ibuprofen (IBU) in the digestive gland tissue of Mytilus galloprovincialis Lam. specimens exposed at low environmental concentrations (250 ng L(-1)) are presented. Using a 1.7 K feature cDNA microarray along with linear models and empirical Bayes statistical methods 225 differentially expressed genes were identified in mussels treated with IBU across a 15-day period. Transcriptional dynamics were typical of an adaptive response with a peak of gene expression change at day-7 (177 features, representing about 11% of sequences available for analysis) and an almost full recovery at the end of the exposure period. Functional genomics by means of Gene Ontology term analysis unraveled typical mussel stress responses i.e. aminoglycan (chitin) metabolic processes but also more specific effects such as the regulation of NF-κB transcription factor activity.
authors.affiliation: CIMA, Faculty of Science and Technology, University of Algarve, 8005-139 Faro, Portugal; CESAM, Department of Biology, University of Aveiro, 3830-169 Aveiro, Portugal.
authors.forename: Vera L
authors.initials: VL
authors.lastname: Maria
authors.affiliation: CESAM, Department of Biology, University of Aveiro, 3830-169 Aveiro, Portugal.
authors.forename: Mónica J B
authors.initials: MJ
authors.lastname: Amorim
authors.affiliation: CIMA, Faculty of Science and Technology, University of Algarve, 8005-139 Faro, Portugal.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Bebianno
authors.affiliation: Department of Science and Technological Innovation (DISIT), Università del Piemonte Orientale Amedeo Avogadro, 15121 Alessandria, Italy. Electronic address: francesco.dondero@uniupo.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Dondero
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D001499:Bayes Theorem; D005786:Gene Expression Regulation / Q000187:drug effects*; D007052:Ibuprofen / Q000633:toxicity*; D049878:Mytilus / Q000502:physiology*; D016328:NF-kappa B / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016328:NF-kappa B; D014874:Water Pollutants, Chemical; D007052:Ibuprofen
keywords: Chitinase; GM2 ganglioside activator; Gene expression; Inflammation; Innate immunity; Mitochondrion; NF-κB