title: Hsa-miRNA-31 regulates epithelial cell barrier function by inhibiting TNFSF15 expression.
abstract: Ulcerative colitis (UC) is characterized by epithelial barrier disruption and alterations in immune regulation but with the etiology unknown. MicroRNA-31 is the most consistent differentially expressed miRNA in ulcerative colitis tissue. The aim of this project is to study the important roles of miRNA-31 in regulation of intestinal epithelial barrier function. We found that expression of miRNA-31 is proportional to the proliferation of Caco2-BBE cells and overexpression of miRNA-31 can increase its trans-epithelial resistance (TER) by decreasing the transepithelial permeability. miRNA-31 can directly bind to the 3-UTR of TNFSF15, thereafter negatively regulating its expression in Caco2-BBE cells. BrdU and TUNEL analysis demonstrated that transfection of miRNA-31 stimulates proliferation or apoptosis-resistance. Taken together, these results revealed a novel mecha-nism underlying the regulation of epithelial barrier function by miRNA-31 during its regulation on proliferation of epithelial cells.
authors.affiliation: Shihezi University Shihezi China.
authors.forename: X
authors.initials: X
authors.lastname: Nan
authors.affiliation: Hangzhou Normal University School of Medicine Hangzhou China.
authors.forename: S
authors.initials: S
authors.lastname: Qin
authors.affiliation: Hangzhou Normal University School of Medicine Hangzhou China.
authors.forename: Z
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Hangzhou Normal University School of Medicine Hangzhou China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Hangzhou Normal University School of Medicine Hangzhou China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Hangzhou Normal University School of Medicine Hangzhou China.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.affiliation: Hangzhou Normal University School of Medicine Hangzhou China.
authors.forename: X
authors.initials: X
authors.lastname: Tan
authors.affiliation: Hangzhou Normal University School of Medicine Hangzhou China yutaoyan@yahoo.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Yan
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D049109:Cell Proliferation; D017097:Electric Impedance; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15 / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C534612:MIRN31 microRNA, human; D035683:MicroRNAs; C117026:TNFSF15 protein, human; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15