title: Increased Ocular Levels of MicroRNA-148a in Cases of Retinal Detachment Promote Epithelial-Mesenchymal Transition.
abstract: The purpose of this study was to determine microRNA expression in vitreous and subretinal fluid (SRF) samples from patients with retinal detachment (RD). The pathological importance of the identified microRNA transcript levels was analyzed in vitro.
  
  
  
  Vitreous fluid was collected from 10 patients with macular hole (MH), vitreomacular traction syndrome (VMTS), or foveoschisis and from 11 patients with RD. Subretinal fluid was collected from 7 patients with RD. Of these, blood serum was collected in 4 patients. MicroRNA microarray profiling was performed to identify microRNA transcripts that were present in vitreous fluid, and more redundantly detected in SRF, of patients with RD, but not detected in control eyes. Western blotting and scratch assays were performed in ARPE-19 cells and primary human RPE cell lines transfected with microRNA to elucidate the effect of identified microRNA transcripts on epithelial-mesenchymal transition (EMT).
  
  
  
  MicroRNA microarray profiling revealed that hsa-miR-148a-3p was the most redundantly detected transcript in SRF and vitreous fluid from patients with RD, but not those with the other diseases. Expression levels of hsa-miR-148a-3p were higher in SRF samples than in blood serum samples in 3 out of 4 patients. Following hsa-miR-148a-3p mimic transfection, ARPE-19 and human RPE cells demonstrated increased expression of α-smooth muscle actin by Western blotting and increased migration ability during scratch assays.
  
  
  
  The results of the present study indicate that hsa-miR-148a-3p was specifically detected in RD and promotes EMT in RPE.
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Takayama
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kaneko
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan 2Laboratory of Bell Research Center-Department of Obstetrics and Gynecology Collaborative Research, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Shiang-Jyi
authors.initials: SJ
authors.lastname: Hwang
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan 3Department of Ophthalmology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Fuxiang
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Higuchi
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Tsunekawa
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsuura
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Iwase
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Asami
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Ueno
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yasuda
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Norie
authors.initials: N
authors.lastname: Nonobe
authors.affiliation: Department of Ophthalmology Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Terasaki
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012313:RNA / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012163:Retinal Detachment / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism*; D055815:Young Adult
chemical_list: C550570:MIRN148 microRNA, human; D035683:MicroRNAs; D012313:RNA