title: Production and Partial Characterization of an Alkaline Xylanase from a Novel Fungus Cladosporium oxysporum.
abstract: A new fungus Cladosporium oxysporum GQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence. C. oxysporum produced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg(2+) improved C. oxysporum xylanase production. Partially purified xylanase exhibited maximum activity at 50°C and pH 8.0, respectively, and showed the stable activity after 2-h treatment in pH 7.0-8.5 or below 55°C. Mg(2+) enhanced the xylanase activity by 2% while Cu(2+) had the highest inhibition ratio of 57.9%. Furthermore, C. oxysporum xylanase was resistant to most of tested neutral and alkaline proteases. Our findings indicated that Cladosporium oxysporum GQ-3 was a novel xylanase producer, which could be used in the textile processes or paper/feed industries.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Guan
authors.affiliation: Department of Chemical Engineering, Beijing Forestry University, Beijing 100083, China; Beijing Biomass Energy Technology Center, State Grid Energy Conservation Service Ltd., Beijing 100053, China.
authors.forename: Peng-Xiang
authors.initials: PX
authors.lastname: Zhao
authors.affiliation: Beijing Biomass Energy Technology Center, State Grid Energy Conservation Service Ltd., Beijing 100053, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Shu-Hao
authors.initials: SH
authors.lastname: Huo
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Feng-Jie
authors.initials: FJ
authors.lastname: Cui
authors.affiliation: Department of Chemical Engineering, Beijing Forestry University, Beijing 100083, China; Beijing Biomass Energy Technology Center, State Grid Energy Conservation Service Ltd., Beijing 100053, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Jiang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D002963:Cladosporium / Q000201:enzymology*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014908:Triticum / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D043364:Endo-1,4-beta Xylanases; D010450:Endopeptidases; C087822:alkaline protease