title: Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit.
abstract: Induction of hyperbilirubinemia in experimental rabbits by phenylhydrazine was optimized in terms of dose, dose interval and number of doses using response surface methodology. Central Composite Design was employed using five levels for each of the three input variables. Degree of hyperbilirubinemia was measured in terms of bilirubin level in serum of animals. A dose dependent significant elevation (P<0.05) of total serum bilirubin level was observed which was optimized by using eight factorial, six axial and six central points as suggested by experimental design. Optimum levels of phenylhydrazine dose, total number of doses and a dose interval to achieve maximum elevation (4.06 mg/dl-1) of total serum bilirubin were found to be 11.56 mg/kg-1body weight, 8 and 24.65 h, respectively. The induction procedure was validated by performing five replicate experiments on a group of five animals which showed 3.56 ± 0.47 mg/kg-1body weight elevation in total serum bilirubin level.
authors.affiliation: Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Punjab, Pakistan.
authors.forename: Haq
authors.initials: H
authors.lastname: Nawaz
authors.forename: Muhammad Aslam
authors.initials: MA
authors.lastname: Shad
authors.forename: Mohammad Saeed
authors.initials: MS
authors.lastname: Iqbal
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D053878:Hemolytic Agents / Q000009:adverse effects*; D006801:Humans; D006932:Hyperbilirubinemia / Q000097:blood / Q000139:chemically induced*; D008297:Male; D010659:Phenylhydrazines / Q000009:adverse effects*; D011817:Rabbits; D011897:Random Allocation; D014580:Ursodeoxycholic Acid / Q000009:adverse effects
chemical_list: D053878:Hemolytic Agents; D010659:Phenylhydrazines; C030299:phenylhydrazine; D014580:Ursodeoxycholic Acid