title: Administration of adipose-derived stem cells enhances vascularity, induces collagen deposition, and dermal adipogenesis in burn wounds.
abstract: Current treatment options for severe burn wounds are often insufficient in reconstructing skin and soft tissue defects. Adipose-derived stem cells (ASCs), a readily available source of multipotent stem cells, represent a promising therapy for the treatment of full-thickness burn wounds. Full-thickness burn wounds were created on the paraspinal region of athymic mice. A one-time, sub-eschar injection of 6.8×10(6) ASCs in PBS or PBS alone was administered at 24-h postoperatively. Time to healing was quantified using Image J analysis. At days 4, 7, 14, and 21, mice were sacrificed and tissues were excised for molecular and histological analysis. ASCs were able to survive in burn wounds as determined by the presence of PKH labeling and human PPARγ expression within the wounds. CD-31 staining demonstrated increased vascularity in ASC-treated wounds at POD 4 (p<0.05). Molecular studies showed enhanced adipogenesis, as well as type III and type I collagen deposition in the ASC treated group (p<0.05). An increase in the mRNA expression ratio of type III to type I collagen was also observed following ASC treatment (p<0.05). By enhancing vascularity, collagen deposition, and adipogenesis, ASCs show promise as an adjunctive therapy for the current treatment of full thickness burn wounds.
authors.affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: blileyj@pitt.edu.
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Bliley
authors.affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: argentaae@upmc.edu.
authors.forename: Anne
authors.initials: A
authors.lastname: Argenta
authors.affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA. Electronic address: satishl@upmc.edu.
authors.forename: Latha
authors.initials: L
authors.lastname: Satish
authors.affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: mclaughlinmm@upmc.edu.
authors.forename: Meghan M
authors.initials: MM
authors.lastname: McLaughlin
authors.affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Aaron
authors.initials: A
authors.lastname: Dees
authors.affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Casey
authors.initials: C
authors.lastname: Tompkins-Rhoades
authors.affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA. Electronic address: marrak@upmc.edu.
authors.forename: Kacey G
authors.initials: KG
authors.lastname: Marra
authors.affiliation: Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA. Electronic address: rubipj@upmc.edu.
authors.forename: J Peter
authors.initials: JP
authors.lastname: Rubin
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002056:Burns / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D020405:Dermis / Q000378:metabolism / Q000473:pathology; D005260:Female; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D047495:PPAR gamma / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D047495:PPAR gamma
keywords: Adipocytes; Adipose-derived stem cell; Burn wounds; Collagen; Vascularization