title: An SNP site in pri-miR-124, a brain expressed miRNA gene, no contribution to mesial temporal lobe epilepsy in an Italian sample.
abstract: Mesial temporal lobe epilepsy (MTLE) is the most common type of refractory epilepsy and is usually associated with hippocampal sclerosis (Hs). The pathogenesis of MTLE involves many biological pathways, some of which seem to be regulated by microRNAs (miRNAs). Increasing evidence shows that single nucleotide polymorphisms (SNPs) or mutations in miRNAs sequence may affect the processing and function of miRNAs and participate in the occurrence of diseases. In this study, the effect of the SNP of one neuronal miRNA, miR-124, on susceptibility to MTLE was investigated using a case control study. To understand the role, a common C/G polymorphism designated rs531564 in the molecular mechanisms of MTLE, we sought to determine whether this genetic variant could influence susceptibility to disease in a cohort of 307 MTLE patients and 306 healthy controls, using TaqMan allelic discrimination assay, on an Applied Biosystems PCR platform. No statistically significant differences were found in the allele or genotype distributions of the miR-124 rs531564 polymorphism among MTLE patients and MTLE-free control subjects (p > 0.05). Our results demonstrate that this SNP has no major role in genetic susceptibility to MTLE, at least in the population studied here.
authors.affiliation: Institute of Molecular Bioimaging and Physiology (IBFM), National Research Council, Section of Germaneto, Catanzaro, 88100, Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Manna
authors.affiliation: Department of Medical Sciences, Institute of Neurology, University Magna Graecia, Catanzaro, 88100, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Labate
authors.affiliation: Department of Medical Sciences, Institute of Neurology, University Magna Graecia, Catanzaro, 88100, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Borzì
authors.affiliation: Department of Medical Sciences, Institute of Neurology, University Magna Graecia, Catanzaro, 88100, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Mumoli
authors.affiliation: Department of Medical Sciences, Institute of Neurology, University Magna Graecia, Catanzaro, 88100, Italy.
authors.forename: Salvatore Maria
authors.initials: SM
authors.lastname: Cavalli
authors.affiliation: Department of Medical Sciences, Institute of Neurology, University Magna Graecia, Catanzaro, 88100, Italy.
authors.forename: Miriam
authors.initials: M
authors.lastname: Sturniolo
authors.affiliation: Institute of Molecular Bioimaging and Physiology (IBFM), National Research Council, Section of Germaneto, Catanzaro, 88100, Italy.|Department of Medical Sciences, Institute of Neurology, University Magna Graecia, Catanzaro, 88100, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Quattrone
authors.affiliation: Institute of Molecular Bioimaging and Physiology (IBFM), National Research Council, Section of Germaneto, Catanzaro, 88100, Italy. a.gambardella@unicz.it.|Department of Medical Sciences, Institute of Neurology, University Magna Graecia, Catanzaro, 88100, Italy. a.gambardella@unicz.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gambardella
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D004833:Epilepsy, Temporal Lobe / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007558:Italy; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: C509207:MIRN124 microRNA, human; D035683:MicroRNAs
keywords: Association studies; Genetic susceptibility; Mesial temporal lobe epilepsy; Single nucleotide polymorphism; miR-124a