title: The DEAD-box RNA helicase 51 controls non-small cell lung cancer proliferation by regulating cell cycle progression via multiple pathways.
abstract: The genetic regulation of cell cycle progression and cell proliferation plays a role in the growth of non-small cell lung cancer (NSCLC), one of the most common causes of cancer-related mortality. Although DEAD-box RNA helicases are known to play a role in cancer development, including lung cancer, the potential involvement of the novel family member DDX51 has not yet been investigated. In the current study we assessed the role of DDX51 in NSCLC using a siRNA-based approach. DDX51 siRNA-expressing cells exhibited a slower cell proliferation rate and underwent arrest in S-phase of the cell cycle compared with control cells. Microarray analyses revealed that DDX51siRNA expression resulted in the dysregulation of a number of cell signalling pathways. Moreover, injection of DDX51 siRNA into an animal model resulted in the formation of smaller tumours compared with the control group. We also assessed the expression of DDX51 in patients with NSCLC, and the data revealed that the expression was correlated with patient age but no other risk factors. Overall, our data suggest for the first time that DDX51 aids cell cancer proliferation by regulating multiple signalling pathways, and that this protein might be a therapeutic target for NSCLC.
authors.affiliation: Department of Respiration; Anhui Clinical and Preclinical Key Laboratory of Respiratory Diseases; First Affiliated Hospital; Bengbu Medical College; Bengbu 233000, Anhui China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gynecological Oncology, First Affiliated Hospital; Bengbu Medical College; Bengbu 233000, Anhui China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Respiration; Anhui Clinical and Preclinical Key Laboratory of Respiratory Diseases; First Affiliated Hospital; Bengbu Medical College; Bengbu 233000, Anhui China.
authors.forename: Chengling
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Respiration; Anhui Clinical and Preclinical Key Laboratory of Respiratory Diseases; First Affiliated Hospital; Bengbu Medical College; Bengbu 233000, Anhui China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 200080, China.
authors.forename: Huanbai
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Respiration; Anhui Clinical and Preclinical Key Laboratory of Respiratory Diseases; First Affiliated Hospital; Bengbu Medical College; Bengbu 233000, Anhui China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000401:mortality / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D016019:Survival Analysis; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
chemical_list: D034741:RNA, Small Interfering; C000626796:DDX51 protein, human; D053487:DEAD-box RNA Helicases