title: Erythropoietin exerts direct immunomodulatory effects on the cytokine production by activated human T-lymphocytes.
abstract: The effect of erythropoietin-β (Epo-β) on the functional profile of activated human T-lymphocytes remains largely unknown, which hinders clinical application of Epo as an immunomodulatory agent. We studied the direct impact of Epo on the activation status of human T lymphocytes following activation by particles loaded with antibodies (Abs) against human CD2, CD3, and CD28. T cell activation was assessed by the surface expression of CD38 activation marker. Epo did not significantly affect activation status of both CD4(+) and CD4(-) T cells, as well as of naive (CD45RA(+)CD197(+)), central memory (CD45RA(-)CD197(+)), effector memory (CD45RA(-)CD197(-)), and terminally-differentiated (CD45RA(+)CD197(-)) T cells. However, Epo markedly augmented production of IL-2, IL-4 and IL10 by activated T cells with concomitant reduction in IFN-γ secretion. Taken together, our data showed that Epo could directly down-regulate pro-inflammatory T cell responses without affecting T cell activation status.
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: N M
authors.initials: NM
authors.lastname: Todosenko
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Shmarov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Malashchenko
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: M E
authors.initials: ME
authors.lastname: Meniailo
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: O B
authors.initials: OB
authors.lastname: Melashchenko
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: N D
authors.initials: ND
authors.lastname: Gazatova
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Goncharov
authors.affiliation: Immanuel Kant Baltic Federal University, Kaliningrad, Russia. Electronic address: seledtsov@rambler.ru.
authors.forename: V I
authors.initials: VI
authors.lastname: Seledtsov
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D007156:Immunologic Memory / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D007155:Immunologic Factors; D011994:Recombinant Proteins; C103998:epoetin beta; D004921:Erythropoietin; D051997:ADP-ribosyl Cyclase 1
keywords: Erythropoietin; Interferon-gamma; Interleukin; T cell; СD38