title: Optimization of prehydrolysis time and substrate feeding to improve ethanol production by simultaneous saccharification and fermentation of furfural process residue.
abstract: Ethanol is a very important industrial chemical. In order to improve ethanol productivity using Saccharomyces cerevisiae in fermentation from furfural process residue, we developed a process of simultaneous saccharification and fermentation (SSF) of furfural process residue, optimizing prehydrolysis cellulase loading concentration, prehydrolysis time, and substrate feeding strategy. The ethanol concentration obtained from the optimized process was 19.3 g/L, corresponding 76.5% ethanol yield, achieved by running SSF for 48 h from 10% furfural process residue with prehydrolysis at 50°C for 4 h and cellulase loading of 15 FPU/g furfural process residue. For higher ethanol concentrations, fed-batch fermentation was performed. The optimized fed-batch process increased the ethanol concentration to 37.6 g/L, 74.5% yield, obtained from 10% furfural process residue with two additions of 5% substrate at 12 and 24 h.
authors.affiliation: Department of Chemistry and Chemical Engineering, Huaiyin Normal University, 111 Changjiang West Road, Huaian 223300, Jiangsu Province, China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Chemical Engineering, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, Jiangsu Province, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Engineering, Huaiyin Normal University, 111 Changjiang West Road, Huaian 223300, Jiangsu Province, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Chemical Engineering, Huaiyin Normal University, 111 Changjiang West Road, Huaian 223300, Jiangsu Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Chemical Engineering, Huaiyin Normal University, 111 Changjiang West Road, Huaian 223300, Jiangsu Province, China. Electronic address: Xiongpengha@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiong
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods / Q000592:standards*; D002138:Calibration; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005662:Furaldehyde / Q000378:metabolism*; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000431:Ethanol; D013395:Sucrose; D005662:Furaldehyde; D002480:Cellulase
keywords: Bioethanol; Cellulase; Cellulose; Ethanol; Furfural residue; Simultaneous saccharification and fermentation