title: Adenovirus infection among allogeneic stem cell transplant recipients.
abstract: The human adenovirus (HAdV) infection can cause severe disease in immunocompromised patients, such as those undergoing allogeneic hematopoietic stem cell transplant (ASCT). The main objective of this study was to prospectively monitor ASCT recipients for HAdV occurrence in a reference center in Brazil, and also to correlate viral positivity, viral load, molecular variant, clinical symptoms, and patients' prognosis. From October/2012 to October/2014, blood and feces of 21 ASCT recipients were screened for HAdV by Nested-PCR. Viral loads were determined by real-time PCR. In total, 57% of the patients had at least one positive sample (serum or feces) for HAdV. Patients presented significantly higher viral load in feces when compared to serum. Positive samples were characterized as HAdVs of species HAdV-C, -D, and -F. The main clinical symptom presented by infected patients was diarrhea, and Graft-versus-host disease (GVHD) was the main intercurrence. An association was observed between HAdV-positivity and diarrhea and also between HAdV-positivity and GVHD. Results from this study may contribute to a better understanding of the HAdV infection pattern in patients submitted to ASCT. Data therein highlight the importance of including HAdV testing during all routine laboratory exams performed on ASCT patients. J. Med. Virol. 89:298-303, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratory of Virology, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Hugo
authors.initials: H
authors.lastname: César Pereira Santos
authors.affiliation: Laboratory of Virology, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Tâmera
authors.initials: T
authors.lastname: Nunes Vieira Almeida
authors.affiliation: Laboratory of Virology, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Fabíola
authors.initials: F
authors.lastname: Souza Fiaccadori
authors.affiliation: Laboratory of Virology, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Divina
authors.initials: D
authors.lastname: das Dôres de Paula Cardoso
authors.affiliation: Araújo Jorge Hospital/Associação de Combate ao Câncer em Goiás, Goiânia, Goiás, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: de Moraes Arantes
authors.affiliation: Laboratory of Molecular Genetics and Cytogenetics, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Hugo
authors.initials: H
authors.lastname: Delleon da Silva
authors.affiliation: Laboratory of Immunoregulation, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Resende Alo Nagib
authors.affiliation: Laboratory of Virology, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Menira
authors.initials: M
authors.lastname: Souza
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D001769:Blood / Q000821:virology; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D005838:Genotype; D006086:Graft vs Host Disease / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D066027:Transplant Recipients; D014184:Transplantation, Homologous / Q000009:adverse effects*; D019562:Viral Load; D055815:Young Adult
keywords: adenovirus; blood; transplantation; viral excretion