title: Inhibition of human norovirus by a viral polymerase inhibitor in the B cell culture system and in the mouse model.
abstract: The recently developed human norovirus (HuNoV) B cell culture and mouse models hold promise for drug discovery and development but their suitability for antiviral studies has not been assessed. We demonstrate the inhibitory effect of the nucleoside analogue 2'-C-methylcytidine (2CMC) on HuNoV replication in the human B cell BJAB cell line and in Balb/c Rag/gamma chain-deficient (Rag-γc(-/-)) mice. These data suggest the applicability of both models for future study and development of antiviral drugs for the treatment of HuNoV infections.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Abimbola O
authors.initials: AO
authors.lastname: Kolawole
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, B-3000, Leuven, Belgium.
authors.forename: Joana
authors.initials: J
authors.lastname: Rocha-Pereira
authors.affiliation: Department of Biomedical and Diagnostic Sciences, University of Detroit Mercy School of Dentistry, Detroit, MI, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Elftman
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, B-3000, Leuven, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Neyts
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA. Electronic address: cwobus@umich.edu.
authors.forename: Christiane E
authors.initials: CE
authors.lastname: Wobus
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000821:virology*; D017250:Caliciviridae Infections / Q000188:drug therapy / Q000821:virology*; D002460:Cell Line; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D029322:Norovirus / Q000187:drug effects* / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents
keywords: Antiviral; Enteric virus; Human norovirus; Polymerase inhibitor; Positive-sense RNA virus